LOCUS       DATALS010000001         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_315475, whole genome shotgun sequence.
ACCESSION   DATALS010000001 DATALS010000000
VERSION     DATALS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315475"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VHS06_00005"
     CDS             <1..576
                     /locus_tag="VHS06_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986549.1"
                     /translation="VKVHPDCHVVIEGSFYSVPYQHVGEELDAYIGERVVEIFRGVEL
                     LTTHTKARRRGEWHTRTEHYPAEKAAYLERTPARCLEIARGIGPKTLEVVETLLSDRP
                     LDRLRSVQSILRLAESVGRERLEAACARSLYYGDGRYRRIKEILNAALDREPLPPEPI
                     PFAPVQQRTESRQYAFARPVSEFFDGSEVVR"
     gene            573..1388
                     /gene="istB"
                     /locus_tag="VHS06_00010"
     CDS             573..1388
                     /gene="istB"
                     /locus_tag="VHS06_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX2986550.1"
                     /translation="MRAMMNASPVHPLLPKLKELKLGGMVDSLELRAAQAVQDGLSPG
                     EFLALLLDDELERRDQRRLGVRLREAGCQDGKTLARFDFAVVPSLNRTLVMELATCAF
                     VARHDNLLICGPTGVGKSHLANSLAFESVRRGYRAIIRSTSQLLAELHASRADGSYSR
                     KIARLDLVDLLVLDDFGLRPLSAQAVDDLYEIISRRYEKRSIAITSNRALEEWAELFG
                     NDLLASAALDRLTHHAQTLIIRGRSYRQLSRRKEVDNQETEIMMDDPAEGLQN"
BASE COUNT          350 a          499 c          567 g          277 t
ORIGIN      
        1 gtgaaggtcc atcccgactg ccacgtcgtg atcgaaggca gcttctactc cgtgccctac
       61 cagcacgtcg gggaggagtt ggacgcctac atcggcgagc gcgtggtgga gatcttccgc
      121 ggagtggagt tgctgaccac ccacaccaag gcgaggaggc ggggggagtg gcacaccagg
      181 accgagcact acccagccga gaaggccgcc tatctggaga ggacaccggc caggtgtctg
      241 gagatagcga ggggcatcgg cccgaagaca ctggaggtgg tcgagacgtt gctgtcggac
      301 cgaccgctgg accggctgcg gagcgtgcag tcgatccttc gcctggccga gagtgtgggg
      361 agagagcggc tagaggctgc ctgtgccagg tccctctact atggcgatgg gcgctaccgt
      421 cgcatcaaag agatcctgaa tgccgccctg gaccgcgagc cgctgcctcc tgagccgatc
      481 cccttcgccc ccgtgcagca gcgcacggaa agtcggcagt acgccttcgc aaggccggtg
      541 tccgagttct tcgacggcag cgaggtggtg agatgagggc aatgatgaac gcgagcccgg
      601 tgcatccgct gctgccgaag ctgaaggagc tgaagctggg agggatggtg gacagcctgg
      661 agctcagggc agcccaggcg gtgcaggacg gcctatcgcc gggggagttc ctggcgctcc
      721 tgctggacga cgaactggag agacgggacc agagacggct gggagtgagg ctcagggagg
      781 ccggatgtca ggatggaaag accctggccc gcttcgactt cgccgtcgtg cccagcctca
      841 accgcacgct tgtgatggag ttggccacct gcgccttcgt ggccaggcac gacaacctct
      901 tgatctgtgg tccgaccggc gtagggaaga gccacctggc caacagcctg gccttcgagt
      961 cggtgaggcg gggctacagg gcgatcatca gatcgacgag ccagttgttg gccgaactgc
     1021 acgcttcccg agccgacggc agctactccc gcaagatagc gaggctggac cttgtggatc
     1081 tgctggtgct ggacgacttc ggactgcggc cgctgtctgc ccaggccgtg gacgatctct
     1141 acgagatcat cagccgaagg tacgagaagc ggtcgatagc gatcacctcc aaccgagcac
     1201 tggaggagtg ggcggagctg ttcgggaacg atctgctggc gagcgccgcc ctggaccgac
     1261 tcacccatca cgcccagact ctgatcattc gaggacgcag ctaccgacaa ctgagccgga
     1321 gaaaggaggt ggacaaccag gaaaccgaga tcatgatgga cgacccggca gagggtctcc
     1381 agaactgatt ccagaaagac cacaaactcg attcttgacg gcctccaaac tcgatttcgt
     1441 ggtctactcc aaggcggcta tgcccggtct acatcgggcg gctattgaca gtctttcgcc
     1501 gaacgcgatt agcagagcca tcgagaacaa cgacgcgagc gcgaccaacc cccagccgga
     1561 tagccaccga gggacgagcc tcgatcgata catcacgtag tagtacatag ccgcaccccg
     1621 tcttgcggaa acacaatgaa gggctagccc tgggggcagt tggtttcagg cttgttgagg
     1681 cggtgttcta cat
//
LOCUS       DATALS010000002         7064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_225419, whole genome shotgun sequence.
ACCESSION   DATALS010000002 DATALS010000000
VERSION     DATALS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225419"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VHS06_00015"
     CDS             <1..503
                     /locus_tag="VHS06_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986551.1"
                     /translation="AEGRAVFGAAADNLIDEANAALKQGMEVLLVVQDGLGGRLATRS
                     TAQQLLHRAPCPLTVVRPERNKVRRRPPNPRKIAREQQPPARDRDIPVAPAVLEGEKD
                     FSMLSVADIARLGNVDEEAVSRCVREDPHFPRPVVHLSTGDLYDRYSIYKWAIEAGLK
                     GLEPAF"
     gene            668..1276
                     /locus_tag="VHS06_00020"
     CDS             668..1276
                     /locus_tag="VHS06_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX2986552.1"
                     /translation="MTLPAWSPIEELAQMRQEIHSAMRQAPGWPVLLPWADDDQYFIP
                     AVDVVSRGEDLIFRLELPGVDPTQDVNVSVEGRKLHVSGRRHVDAEQSETAYYFQGMS
                     YGRFDRTFTLQDDINEKAITAEYNNGVLEVKVAGGARRSTTPKGTSIPVKVPEEATPQ
                     EPAASESGSETVNETRSETGSETESEASPEAAPARVQIKTKA"
     gene            1457..2179
                     /locus_tag="VHS06_00025"
     CDS             1457..2179
                     /locus_tag="VHS06_00025"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX2986553.1"
                     /translation="MSPSTSNSSFWIDLLLIVPVLLAWWSQARVRHAFREADQIQNEE
                     HISGYETARYLLDESGLGTIPVVVGQGTLSGDSDAYDPVAKRVVISKQTASHSTDLSV
                     GVAGHEVGHATQDAEGFPLMRLRTSIANWLIRVSWVSSFAFVGGFLLGIVPLMWVTVG
                     IMGVQVLFALVTLPVEINASGRAIKLLERGRLIALSEKRSVQKVLRAAAFTYLASAAQ
                     TLAFFLFCVIVLAAAVGLQLPR"
     gene            2176..3249
                     /locus_tag="VHS06_00030"
     CDS             2176..3249
                     /locus_tag="VHS06_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2986554.1"
                     /translation="MSSHPLRRDLRRPVDSRPRPIGAGNWLRTHWLAILGWCISAAGL
                     WYVISRTDLSILKEDLADVSWGLIVAAVILQVVPKVLEAVRWQSLLRPFSARFWRLLR
                     ATYIGCLCSTILPLSAGELVRATLVTEDTGATLPEVLSTEAVERVVDTAAVVLVAWFA
                     LRGVVLPRNLLIGQIVLAVAAIVLVVAFVLIVIRRADLIARMTRWRASNRLSNSVRSA
                     GLEFVQTIGRVRSRAIQLTAVASLGTTAVNIATYWLLLRAYHVPLSGAQAAALFAIVA
                     IGTALPGAPGDVGSWQFFCAVGLELFGVSSGQATGFSLVGWAVFAIPPMLIGVVVLLA
                     SSAKWSQLVAIRPPAEARAAVEG"
     gene            3514..3876
                     /locus_tag="VHS06_00035"
     CDS             3514..3876
                     /locus_tag="VHS06_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX2986555.1"
                     /translation="MADAITGFAGSMNFVYINAAVFALWMLLLERAPWPTLTLAVSLE
                     AIFLSAFVMISQNRADAKRQVLADSQWEYVQTEERQNEELLRLSNEILELTRAIHVLT
                     TQTYGTAAPPIAGGSETP"
     gene            complement(4219..6039)
                     /locus_tag="VHS06_00040"
     CDS             complement(4219..6039)
                     /locus_tag="VHS06_00040"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX2986556.1"
                     /translation="MASEQRSIGGGRATKPWFWIAVALGALVAICLLAIVVDSAIYYG
                     KVHSGVRLSGVDVGGKTRDQATAALNALVQEAESRPITIAWGDKSWAVMPEDGATRMD
                     VEAAVNTAMQETRKNGFLTDILARWRLYFSARDFPLTGSVDKSKLAEFVAGVAKQVDI
                     EPVNAGLRIEKTSVKVVDSVEGKAVDQAALIERLSNLLATLQSTTVDLPVATKEPEVT
                     AEDNAEAQKQAETMISGPVRVSDGAKTWTLGAEDIASYMTFKSEIKNGVSTLVPYLDA
                     SGLQPLLDEIAPAVAKEPVDASFASDGGKAWVVPGKNGQRLDPDATAEAITAATLSPI
                     NRKVQIVLSTKEPALTTAEAEAMGLKEKLASYTTRYVGSPDRQANVRKATEYGTNVFL
                     APGEIYDTDKQFGPRTPARGYRLAPGITGPNTLEDVLGGGICQVSTTLFNAVFFAGLD
                     VVERWNHSIYIDHYPKGRDATVTGGGKNLRFKNDTGHYVWIRGTSDGITTTITIYGTN
                     DGRKVSFTTSDFYNVKSRTVETVADPSLRAGRTLVVDQGQTGRSVKVVRTVTRADGRG
                     IHKDSFVSTWLMYPKEIRVGTATTASSTSTTTPTSILPSS"
     gene            complement(6380..>7064)
                     /locus_tag="VHS06_00045"
     CDS             complement(6380..>7064)
                     /locus_tag="VHS06_00045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX2986557.1"
                     /translation="GPWTGNTHLAPATDALDTAKDDYNRLHDGVALLQEQERRQREEA
                     QKRAEAEAAAKAAAARAAASRAADARRPPAKAPAKPAAKRNAAVAGAKWVFPVQGPNS
                     FSHSFGAPRYAGGYHTHKGVDIMAPRGTPVVAVTAGVIRSTSPVERGLGGITIHLRGS
                     DGNVYYYAHLSSVKNGIAPGVRVKAGQLIGYVGNTGDARSTAPHLHFEIRPGGGAAID
                     PYPTLIKYR"
BASE COUNT         1230 a         2200 c         2224 g         1410 t
ORIGIN      
        1 aagccgaggg cagggcagtg tttggcgcgg cggccgacaa cctcatagat gaggcgaatg
       61 cggctctgaa gcagggtatg gaagtactcc tggttgtcca ggatgggctg ggcggccggt
      121 tggcaactcg ctccaccgct cagcagctct tgcaccgcgc cccctgtccc ctgaccgtag
      181 tccgccccga acggaacaag gtccgtcgcc ggccgcccaa cccacgcaag attgcccgcg
      241 agcagcagcc gccggcgcga gaccgggaca tccccgtcgc gccggccgta ctggaaggag
      301 agaaggactt cagcatgttg agcgtggccg acatcgccag gctcggtaac gtcgatgaag
      361 aggccgtcag ccggtgtgtc cgcgaagatc cgcacttccc gagaccggtg gtgcatctct
      421 ccacgggcga cctctacgat cggtactcaa tctacaagtg ggcgatcgag gccggactga
      481 agggacttga gcctgccttc tgacctgcgg gcgtgtggct ggccattgct ctcctcgcag
      541 agggggaagg ctactacctt ccccctctgc ttcttcattg gttcgttcat acgttccggt
      601 cgatgcccac tcatcccttc cggatcgata ctcgtctcgt ctaggaatca cgagaaggag
      661 gcgaaccatg acactaccag cttggagtcc catagaagaa ctggcccaga tgagacagga
      721 gattcactcg gccatgcggc aggccccggg ttggccggta ctcttgcctt gggcagacga
      781 tgaccaatac ttcatcccgg ccgtcgatgt cgtcagccgg ggtgaggact tgatcttcag
      841 gttggagctc cccggggtcg atcccactca ggatgtgaac gtctcggtcg aggggaggaa
      901 gctgcatgtt tccgggcggc gtcacgtgga cgccgagcag agcgaaacgg cctactactt
      961 ccagggcatg tcctacggca ggtttgaccg gaccttcact ttgcaggacg acatcaacga
     1021 gaaagcgatc actgccgagt acaacaatgg tgtcctcgaa gtcaaggtgg ctgggggagc
     1081 gaggcgatcg acgactccca agggcacgag cattccggtg aaagtgccgg aggaggccac
     1141 ccctcaggag ccggccgcaa gcgagtctgg gagcgagacc gtgaacgaga cccgcagtga
     1201 gaccgggagc gagacagaat ccgaagcgtc acccgaagcg gccccagcac gggtgcagat
     1261 caagacgaaa gcctgacgct cagaagggca gacaggcgat cggttgcggc ccccgaagtc
     1321 gagcggctcc gggggccgca ccatcttccc cctttgactg aggaatcgct cgattcggac
     1381 gatgcccgct cacaccgagg ggggcgagaa tcgaagccag gaagcagccg acgacatcga
     1441 ggctcttgga ggaaacatgt cacccagtac gtcgaactca tctttctgga tcgacctgct
     1501 gctcatcgtc cctgtgctcc tcgcctggtg gtcgcaggcc cgagtcaggc atgcctttcg
     1561 ggaagccgac cagatccaga atgaggagca cataagcgga tatgagacgg cccgctatct
     1621 cctcgacgaa tccggcctgg gcaccattcc ggtagtggta gggcagggca cattgtccgg
     1681 agacagtgat gcgtacgacc cggtcgcgaa gcgagtggtt atctccaaac agacggcgtc
     1741 gcatagcacc gatctcagcg tcggcgtcgc cggccacgag gtgggtcacg ccacccagga
     1801 cgccgagggg ttcccgttga tgcgtctgcg cacctcgatc gcgaactggc tcatacgagt
     1861 gtcgtgggtc agttcctttg ccttcgtcgg cggtttctta cttgggatcg tccccctcat
     1921 gtgggtgaca gtcgggatca tgggcgtgca agtgctattt gcgctggtca ccctgcccgt
     1981 ggagatcaat gccagcggga gggcgataaa gctgctcgaa cgcggccgtc tcatcgctct
     2041 ctcggagaag cgcagcgtgc agaaggtcct gcgcgcggcg gcctttacgt acctcgcctc
     2101 cgccgctcag actttggcgt tcttcctgtt ctgcgtaatc gttctggcgg ccgccgtagg
     2161 tctccagctg ccgcgatgag cagccacccc ctcagacggg acctccgccg cccggttgac
     2221 agccgtcccc gaccaatcgg cgcgggaaac tggcttagga cgcactggct ggccatcctc
     2281 ggctggtgca tctcggccgc cgggttgtgg tacgtcatct cgcggacaga cctgtccatc
     2341 ttgaaagaag atctagctga cgtcagctgg ggcctgattg tcgccgcggt cattctccaa
     2401 gtggtgccga aggttcttga agctgttcgc tggcagtcgc ttctccgccc gttctcggcg
     2461 cggttctggc gcctgcttcg agcaacctac atcgggtgtc tgtgcagcac catcctgcct
     2521 ctgtccgctg gagaactagt gcgagcgacg ctcgtcaccg aggatactgg tgcgaccctg
     2581 cctgaagtct tgtctaccga ggcagtagaa cgggtggtgg atacggcggc tgtcgtgctg
     2641 gtcgcgtggt ttgctctccg cggggtggtc ctgcctcgga acttgctcat cggtcaaatc
     2701 gtgcttgcgg tcgccgcgat agtgttggtc gtagctttcg tgctcattgt catccggcgc
     2761 gccgatctca tcgccagaat gacccggtgg cgtgcatcta atcgcttgtc caacagtgtg
     2821 aggtcggcgg gactcgaatt cgtccagacc atcggccgcg tacggtcgcg cgcaatccag
     2881 ctgaccgccg ttgcgtcgct tggcactacg gctgtcaata tcgcaaccta ctggctgttg
     2941 cttcgtgcgt accatgttcc tctgtcgggc gcccaggccg cagcactctt tgccatcgtc
     3001 gcgatcggta cggcgctccc cggagctccc ggcgacgtcg gctcatggca attcttctgc
     3061 gcagtgggcc tggagctgtt cggtgtctct tccggtcagg cgaccggctt cagcctcgta
     3121 gggtgggcgg tcttcgcgat cccgccgatg ctcatcggcg tcgtcgtcct ccttgcatcc
     3181 tcagccaagt ggtcgcaact ggtggccata cggccgccgg ccgaggctcg cgcagccgtt
     3241 gaaggttgag gcatgcgcgc tcgagacgga cgggacgctg gtaggccgat caggaccgat
     3301 ctttcaccct atccggtcga tgcccattca tactcgcggg tgcgaatctg gcggcatgag
     3361 aaccgtaact accgagcgat gccacgagcg acacagcgat gaactcttcc gggattagga
     3421 ggacacatga gcgcacgagc gattaggttg ccaccgaaga acccggccct gcgccaggac
     3481 gcaaaggagc gggcccagag cgtgcagaat cggatggccg atgccatcac cgggttcgcc
     3541 gggtcgatga acttcgtcta tatcaacgcc gcggtgttcg ccttgtggat gctgctgctc
     3601 gagcgagcgc cctggccgac cctgacgctg gccgtttctc tggaagcgat cttcctctcg
     3661 gccttcgtga tgatcagcca gaacagggcc gacgcgaagc ggcaggtgct ggccgacagt
     3721 cagtgggaat acgtccagac cgaagagcgg cagaacgaag agctcctgcg actgtcgaat
     3781 gagatcctag agctgaccag agcgatccac gtgctcacca cgcaaacgta tgggacggcg
     3841 gcgccgccga tcgctggcgg gtccgaaaca ccgtagggtt cgctgccgtt cagctgccgt
     3901 ccgactatgc acggaggccc gagaacgcgg cagattccga gtgagcacga gagggcagga
     3961 gcagccgccg atacgattac ctacggcgaa ctggggaccg cagcgacagg agcggagtcc
     4021 catggtcgtg acaaacgggt tctacgaagt gcaattccag aaaaggcagt gccccggcac
     4081 gaaatcgcgc cagggcactt ggggtgcaaa agaggcggag gagaagtgcc tcattagcta
     4141 gggaatagcc taacggagca ctgtaaagtc ctgtcgaaaa gcctgtaaaa ggttggtaaa
     4201 ggtcccagcc gccatcgatc acgacgaggg gaggatgctc gtcggagtgg tcgtggaagt
     4261 actgctggct gtggtcgctg ttcccacccg gatctccttg ggatacatca gccaggtgct
     4321 gacgaaggag tctttatgta tgccccttcc gtctgcgcgg gttaccgtcc gaacgacctt
     4381 gacggagcga cccgtctggc cctggtccac gaccagggtc cggcctgccc gtagcgacgg
     4441 atcggcaacc gtctcgaccg tgcgtgattt gacgttgtag aagtcgcttg tcgtgaagga
     4501 gaccttccgt ccgtcattcg tgccgtagat ggtgatcgtg gtcgtgatcc cgtcggaggt
     4561 ccctcggatc catacgtagt gccccgtgtc gttcttgaag cgaaggttct ttccgccacc
     4621 cgtcaccgtc gcatcgcggc ctttcggata gtggtcgatg tagatcgagt ggttccaccg
     4681 ctccaccacg tcgaggccgg caaagaagac agcattgaat agtgtcgtgg aaacctggca
     4741 gatgccgccg cccagcacgt cctccaaggt gttcgggccg gtgatgccag gcgccagccg
     4801 gtaaccgcga gccggggtcc tcggcccaaa ctgcttatcg gtatcgtaga tctcgcccgg
     4861 ggcaagaaac acgttggtgc cgtactcggt cgccttccgg acattcgcct gcctgtctgg
     4921 ggagcctaca tacctggtgg tatagctcgc gagcttttct ttgagtccca tcgcttcggc
     4981 ctctgcagtc gtcagggccg gctccttggt gctgaggact atctgcacct ttcggttgat
     5041 aggcgaaagt gtcgctgcgg ttatcgcctc agccgtcgca tcgggatcca gccgctgccc
     5101 gttcttgccc gggaccaccc aggccttgcc gccatcactt gcgaatgaag catcgacagg
     5161 ctctttggct acggccgggg cgatctcatc gaggaggggc tgcagtccgc tcgcgtccag
     5221 gtagggcacg agcgttgaca caccattctt tatctccgat ttgaaggtca tgtaagaggc
     5281 gatgtcctcg gcgccgagcg tccatgtctt ggcgccatcg cttactctga caggaccgct
     5341 gatcatggtc tcggcctgct tctgcgcttc ggcgttgtcc tccgcggtga cttcgggctc
     5401 cttcgtggcc accggcagat cgaccgtggt gctctgcagt gtcgccagaa gattgctcag
     5461 tcgctcgatg agggcagctt ggtcgacagc tttgccttcg acgctgtcca cgaccttcac
     5521 agacgtcttc tcgatccgga ggccagcgtt caccggctca atatccacct gcttggctac
     5581 gcccgcgacg aactccgcca gcttcgattt gtcgaccgaa ccggtcagcg ggaaatccct
     5641 cgcgctgaag tacagcctcc atcgagcgag gatgtcggtg aggaagccgt tcttgcgtgt
     5701 ctcctgcatc gcggtgttca ccgcagcctc tacatccatt ctggtggctc cgtcctccgg
     5761 catcacggcc cagctcttgt ctccccaggc gatagtgatc ggtctgctct ctgcctcttg
     5821 aaccaaggcg tttagagcgg cggttgcctg gtccctggtc ttgccaccta cgtcgacgcc
     5881 ggacaggcgt actccggaat gtaccttgcc gtagtagatg gccgagtcga ccacaatggc
     5941 gaggaggcat atggcgacga gggcgcccaa ggcgactgca atccaaaacc agggcttcgt
     6001 agcgcgcccg cccccgatgc tccgctgctc gctcgccaac gccgtactcc tagtcgatcg
     6061 gagattgtcc caacggtgat gctatccccg tgacagcgcg aggccctggt ccgttgggtc
     6121 gccgaccggg gccggggcgc gtgaggctcg tttgcggggt gcgtccacgc ctctggacgc
     6181 actctaagtg ccccctgcaa agatagtttg gaggtcctgt aaaagatcgg taaaagtggc
     6241 caggtacgag ggtccacgga ttggaggccc tggtccccgt ggaagtggac caaggcctcc
     6301 tcgagacaga tgaggctctc cttgcgatgc tgagcctctg agatggcccc agactacgaa
     6361 ccgggtatcc ccggcggagt catcggtatt tgatcagcgt cgggtacggg tcgatcgcgg
     6421 cgccgccacc cggtctgatc tcgaagtgca agtgaggcgc ggtacttctc gcgtcgccgg
     6481 tatttccgac gtacccgatg agctgtcctg ccttcaccct gacccccggg gctatcccgt
     6541 tcttgacgga gctcaggtgg gcatagtagt agacgttccc gtcgctgccg cggaggtgga
     6601 tcgtgatgcc acccaagcct cgttccaccg ggctcgtact gcggataact cccgcagtca
     6661 ccgccactac gggcgttccc cgaggtgcca ttatgtcgac gcccttgtgt gtgtggtatc
     6721 caccggcata cctgggggcg ccgaagctat gcgagaagct attcggcccc tggaccggga
     6781 acacccattt ggcccccgcc accgccgcgt ttctctttgc cgccggcttg gctggagctt
     6841 tcgcaggcgg acgcctggcg tcggctgccc tgctggccgc cgcccttgcg gccgctgcct
     6901 tcgctgccgc ctcggcttcg gcccgcttct gggcctcctc ccgctgtctc cgctcctgct
     6961 cctggagcag tgcgaccccg tcgtggagcc ggttgtagtc gtccttggcg gtatcgagcg
     7021 cgtcggtggc gggggccaaa tgggtgttcc cggtccaggg gccg
//
LOCUS       DATALS010000003         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_180356, whole genome shotgun sequence.
ACCESSION   DATALS010000003 DATALS010000000
VERSION     DATALS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180356"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VHS06_00050"
     CDS             <1..570
                     /locus_tag="VHS06_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2986558.1"
                     /translation="IPLATMVVSAVTLKEPLGVKSVGALAIGAVGVFVVVSQRGIGVA
                     MDGGFLFGSVCLVANAVCFAFYSVLIRGIRGRYSSLTVTAGMMIAGTLGLLLLSFFTE
                     DWRTITVLSALQWWAILYLAVVCSVLAYFFYNFALTRVEAGKAAVWLYLEPPVSVLLG
                     AAMLGEAIAVQTVVGGLLILASLLLTNRT"
     gene            complement(628..804)
                     /locus_tag="VHS06_00055"
     CDS             complement(628..804)
                     /locus_tag="VHS06_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986559.1"
                     /translation="MGWKDVNVVQYSTADSLMDADLMWRCRPHRIYAFALADLNELKA
                     ARRSAALSSSKVRM"
     gene            839..1975
                     /locus_tag="VHS06_00060"
     CDS             839..1975
                     /locus_tag="VHS06_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX2986560.1"
                     /translation="MSKLGTELTIKGMHLRNRLVLPPITGNYASTDGEVTPELIGFYR
                     QRARQVGLAIVEAAAVRGDGRITPRSLGIWHDGQLPGLRELAGAIKAEGAAAVIQLGH
                     AGAGSVPVDGGIKGASPSGVRTKEGNEATALSTEQIAEIRVDFVAAALRAKAAGFDGV
                     EIHGAHLYLLSQFMSPLTNQRTDGYGGDLRRRMALPLEVVRGIRGQAGDDFVILFRMN
                     TVEMIEGGMTVADAGNVAMLLEDAGVDVIHASLVAQGDWIEDDMGRFLTSSSRLAKER
                     PFGANLANVARVTAMVGVPVIAVGKCADSGVAEQALADGVSDMIAIGRQMIADPKAPE
                     KLIAGRDDEIVRCTECMGCFACIGKGRPVVCSVNKNPTGEPEYA"
BASE COUNT          359 a          619 c          698 g          400 t
ORIGIN      
        1 atccccctgg ccacgatggt ggtctcggcg gtcaccttga aggagccgtt aggggtcaag
       61 agtgtcggcg cacttgcgat tggggctgtg ggcgtgttcg tggtggtcag ccagcgaggg
      121 atcggcgttg ccatggatgg aggctttctc ttcggcagtg tctgtcttgt cgcgaacgca
      181 gtctgcttcg ccttctattc cgtcctcatc cgcggcatcc gaggccgcta ctcttcactc
      241 acggtgacag ccgggatgat gatcgcgggt acgctggggc tgctcctact ttccttcttc
      301 accgaagatt ggcggacgat caccgtcctt tctgccctcc aatggtgggc cattctctac
      361 ctggccgtgg tttgttcggt actggcgtac ttcttctaca acttcgctct cacgcgggtg
      421 gaagccggca aggccgcggt ctggctctac ctcgagccgc cggtgtccgt tctcctcggt
      481 gccgcgatgc tgggtgaagc catcgccgtc cagacagttg tcggcgggtt gctgatcctg
      541 gcgagcctcc tgctgaccaa ccgaacgtag cccgcttgca cctcttgaca tccccgcgtc
      601 acgatcgcct gcgtgggcct atctgttcta cattctgacc ttcgacgagg acaatgccgc
      661 cgatctccgt gcggccttca actcgttcag gtcggccaag gcaaacgcgt agatgcggtg
      721 aggtcggcac cgccacatga ggtccgcgtc catgagggaa tcggcggtgc tatactgaac
      781 gacattcacg tccttccaac ccattctcgc agcttctgta acaagaggag tggtgctgat
      841 gtcgaaactg ggcacagaac tgaccataaa gggcatgcac ttgcgcaaca ggctggtgct
      901 tcccccaatc acagggaact acgcctcgac cgacggagag gtgacacctg agttgatcgg
      961 gttctatcga cagagggctc gacaggtggg gttggccatc gtcgaggccg ccgccgttcg
     1021 gggcgatggt cggatcacac ccaggagcct cggcatctgg cacgatggcc agcttccggg
     1081 actgagagag ctggcggggg ccatcaaagc cgagggcgca gccgcggtta tccagttggg
     1141 gcatgccggg gccggatctg tgcctgtgga cggcggcata aagggagcat cgccgtccgg
     1201 ggttcgcacc aaggaaggca atgaggccac ggccctatct acagagcaga tagccgagat
     1261 cagggtggac ttcgtggcgg ctgcccttcg cgccaaggca gcggggttcg acggcgtcga
     1321 gatacacggg gctcacctct accttctgag ccagttcatg tcccccctca cgaaccagag
     1381 aaccgatggc tatggaggag acctgcgacg caggatggct ctgccgttgg aggtggtgcg
     1441 agggatccga gggcaggccg gcgacgactt cgtaatcctg ttccggatga acactgtaga
     1501 gatgatcgag ggcgggatga ccgtggcgga cgccggcaat gtcgcgatgc tgctggagga
     1561 tgccggggtg gacgttatcc acgcttcgct ggttgcccag ggtgattgga tagaggacga
     1621 tatgggacgc ttcctcacgt cctcgtctcg cctagcgaag gagcggccct ttggtgccaa
     1681 cctggcgaac gtcgcccggg ttacggcaat ggtcggcgtc ccggtgatcg ccgtcgggaa
     1741 gtgcgcggac agcggggtcg ccgagcaggc cctcgccgac ggcgtgtcgg acatgatcgc
     1801 catcgggcga cagatgatcg ccgacccgaa ggcaccggag aagctgatcg cggggcgcga
     1861 cgacgagatc gtccgctgca ccgaatgtat gggctgcttt gcctgcatcg gcaaggggcg
     1921 gccggtggtc tgctcggtga acaagaaccc gaccggtgag ccggagtacg cctgatcggc
     1981 actccagccc agcgcgccac tgggacatag ggcggggcgc tcggccccgc cgccatcgca
     2041 tggatatgac caccgggcgg cagggcacag gcgtgg
//
LOCUS       DATALS010000004         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_286,
            whole genome shotgun sequence.
ACCESSION   DATALS010000004 DATALS010000000
VERSION     DATALS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..585)
                     /locus_tag="VHS06_00065"
     CDS             complement(67..585)
                     /locus_tag="VHS06_00065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2986561.1"
                     /translation="MGTGDWVRIAPDVHLGRDVQVFGFVNLYGCEIGDEAKVGTFVEI
                     QRGAKIGRRVKVSSHTFICEGVTIEDEVFIGHGVMFTNDKYPRATTEDGALQTGSDWE
                     VIPTFVKRRASIGSNATILCGVTIGEGAIVGAGSVVTKDVPPYAVVAGNPARVRRKMI
                     DSRLRHERGSQP"
     gene            complement(510..1595)
                     /locus_tag="VHS06_00070"
     CDS             complement(510..1595)
                     /locus_tag="VHS06_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2986562.1"
                     /translation="MIKVGVVGCGYWGPKLARNFHEMPEAKLEWVCDFRKDRLDHMRH
                     LYPEARTTTSYEKVLASDVDAVVLATPVSTHCPLALLALGARKHVLVEKPLAASAREA
                     RSIAVAAHRLGLIAMVGHTFVYNPAVEAVKEIIDSGELGHIYYINGMRVNLGLFQPDI
                     NVLWDLAPHDISILMYILGSDPVAVSARGGVFVQKAKGVHDMAYVSARFPGNILADIR
                     VSWLDPVKYRLYTVVGSRKMLVYDDIAAVDKVVVYDKRVEFPPYSDTEQEFHASYFSG
                     EGVPHPVVWSEPLRRQCEHFVQCIEQKRVPRSSAEAGLKVVQVLEAADRSLHNGNSWE
                     DIEWGLGTGSGSRQMFTLDGTSRSLAS"
     gene            complement(1621..2298)
                     /locus_tag="VHS06_00075"
     CDS             complement(1621..2298)
                     /locus_tag="VHS06_00075"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX2986563.1"
                     /translation="MSSRGVVAPTILVDDEHRSYLRLKRAADVVLSAVALLVLSPVLL
                     ALAILIKLHSPGPVLFRQTRVGKDGKPFTFLKFRSMRPNADVSIHRDHVAALIAGHDQ
                     APTNGKSAKLERDPRITGIGRVLRKTSIDELPQLINVLRGEMSLVGPRPPIPYEVELY
                     EEWYKRRFEALPGITGWWQVMGRNRVSFDDAMKLDIYYVDHMSLWFDLRILVLTPFAV
                     LTGNGAG"
     gene            complement(2449..>3082)
                     /locus_tag="VHS06_00080"
     CDS             complement(2449..>3082)
                     /locus_tag="VHS06_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986564.1"
                     /translation="LLDELRERGFEVGVHGLKHDGKLFSSRATFEKRAQHINRYLDEW
                     GAVGFRSPMTHRNPEWMQMLDVEYDASFFDTDPFEPMQGGTMSIWPFMMGRFVELPYT
                     LVQDHTLMMTLGETTPRVWLEKVAFIRRHSGMALSLTHPDYLCEQSRLAVYQQFLRAV
                     RERGDYWHALPREVARWWQARAAAESVADLPGGNDRGDPVGRWRHRVATR"
BASE COUNT          605 a          981 c          857 g          639 t
ORIGIN      
        1 tatccagcac aaccaggggg ctcgacacgg actctgatcc tggcaggcgg tcgtaattct
       61 ctttggtcat ggttgtgatc ccctctcatg ccggagtcta ctatcgatca tctttctccg
      121 aaccctcgcg gggttcccag cgacaactgc atagggcgga acgtcctttg ttaccacact
      181 gccagcgccc acgatagccc cttcgccgat agtcaccccg cataggatgg tcgcgttgct
      241 gccgatggaa gcccgtcgct tcacgaatgt gggtattacc tcccagtcac tccccgtctg
      301 aagtgccccg tcttccgtgg tggcgcgcgg gtacttgtcg ttggtgaaca tcactccgtg
      361 gccgatgaag acctcatcct cgatagtcac gccttcacag atgaaggtgt ggctcgagac
      421 tttcacacga cggccgatct tcgcccctcg ctgtatctcc acgaatgtcc cgaccttggc
      481 ctcgtcgccg atctcgcagc cgtacaggtt tacgaagcca aagacctgga cgtcccgtcc
      541 aaggtgaaca tctggcgcga tcctgaccca gtccccagtc cccactcgat atcctcccag
      601 ctgtttccat tatggagcga gcgatcggct gcctccagca cctgaaccac cttgagtccc
      661 gcctcggcgc tgcttcgcgg cacccgtttc tgctcgatgc actgcacgaa atgctcgcac
      721 tgtcggcgaa ggggctccga ccacaccacc ggatgaggta ccccttcccc actgaagtag
      781 gaagcgtgaa actcttgctc ggtgtcggaa tagggcggaa actcgacccg cttgtcgtag
      841 accacgacct tgtcgaccgc ggcaatatcg tcgtagacga gcatcttcct gctgccgacc
      901 acggtgtaca gccggtactt caccggatcc agccaactca ctcgaatatc ggccaggata
      961 ttaccaggga atcgcgcgct cacgtaggcc atatcgtgaa cacccttggc cttctgcacg
     1021 aacacgccgc ctcgagcgct tactgcgacg ggatcgctgc ccaggatgta catgaggatg
     1081 gagatatcgt ggggggccaa atcccacaac acgttgatat ccggttggaa gagtcccaag
     1141 ttcactctca taccgttgat gtagtagatg tggcccagtt cgccgctatc gatgatctcc
     1201 ttcacggcct ccacggcagg gttgtagacg aaggtgtgac ctaccatggc gatcaatccc
     1261 aggcggtgag ccgcgacggc tatggatctg gcctctcgag ccgaggctgc gagtggcttt
     1321 tcaaccagca catgctttct tgcccccaga gcaagcagcg caagcggaca gtgagtggaa
     1381 accggggtgg ccaaaaccac tgcgtctacg tcggaggcca gcaccttctc gtagctggtg
     1441 gtggttcggg cttccggata gaggtgccgc atgtggtcca ggcgatcctt ccggaagtcg
     1501 cacacccatt ccagcttcgc ctcgggcatc tcgtggaagt tcctggccag cttgggaccc
     1561 cagtagccgc acccgactac tccgaccttg atcatggtat taccccctga ctctgccgga
     1621 ttaccctgca ccgttgcctg tgagtacagc gaaaggtgtc aggaccagga ttctgagatc
     1681 gaaccataga gacatatgat ctacgtagta gatgtccaac ttcattgcgt cgtcgaagct
     1741 caccctgttg cggcccatca cctgccacca tccggtgatg ccgggtagtg cctcgaagcg
     1801 gcgtttgtac cattcctcat acaattccac ctcgtacggt atcggaggtc tcgggccgac
     1861 gaggctcatc tcaccccgca atacgttgat cagttgagga agctcgtcta tgctggtctt
     1921 gcggaggact cgtccgatcc ctgtgatcct ggggtctcgc tccaacttcg cactcttgcc
     1981 gttggtcggc gcctgatcgt ggccggctat cagtgcagcc acatgatccc tgtggattga
     2041 gacgtcggcg ttggggcgca tggagcgaaa cttgagaaag gtgaagggtt ttccatcctt
     2101 accgactcgg gtctggcgaa agagcacggg accgggcgag tggagcttga ttaggatcgc
     2161 cagagccagc agaacgggcg acaggaccag cagggccacc gcgctcagca ccacgtccgc
     2221 ggctctcttt aggcgcagat agctgcggtg ttcatcatcc accaggatgg taggggcaac
     2281 aactcctcgg ctcgacatct cacattcctt tgaaatctca gacggggtcc gcaaagcgga
     2341 tcactacttc gtcagaatat gcctcaggag gcctgtcaac cttgccgcat cgtgccggcg
     2401 gcaccaaccg agtggatgat tctgtccgga cccctctgta taaccgcttc agcgggtagc
     2461 aactcgatgc cgccatcggc caaccggatc tccccgatcg ttcccccccg gcagatcggc
     2521 gaccgactcg gccgcggctc gggcttgcca ccagcgagcc acctctcgcg gcagggcgtg
     2581 ccagtaatcg cctcgctccc gcacagcacg caggaactgc tggtacacgg ccaggcggct
     2641 ctgctcgcac agatagtcgg gatgggtcag ggacagtgcc atcccactgt gtcgcctgat
     2701 gaacgccacc ttctcaagcc acaccctggg cgttgtctcc cccaaagtca tcatcagggt
     2761 gtggtcctgg acgagggtat agggcaactc gacgaagcgc cccatcatga atggccagat
     2821 gctcatcgtt cccccctgca tcggctcgaa ggggtcggtg tcgaagaacg aggcatcgta
     2881 ctcaacatcc agcatctgca tccactcagg gttacggtgg gtcatcggag accggaagcc
     2941 tacggcgccc cactcgtcga gatagcggtt gatgtgttgg gcacgctttt cgaaagtcgc
     3001 cctggaagag aacagcttgc cgtcgtgctt taggccgtgc acacccacct cgaagccgcg
     3061 ctcccgtagc tcgtcgagaa ga
//
LOCUS       DATALS010000005         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_135405, whole genome shotgun sequence.
ACCESSION   DATALS010000005 DATALS010000000
VERSION     DATALS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135405"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VHS06_00085"
     CDS             <1..995
                     /locus_tag="VHS06_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX2986565.1"
                     /translation="APQPTAAPAKATSAPAASSAAPTNAAAPAKVQHVKLSDQAAEDH
                     PEQKAFRDFAARVKELTNGAVIVDLFPNSVLGSPRGNIEQMQLGQLEFLKISTAALGG
                     FVPQYQVFDLPYVFQSREHLLSKTDGEVGQTLSKLLESKLSIKTLGYFDAGARSIFNS
                     KKPINTAADLKGMKIRVMESPLMIQTFNSMGALATPMAAGELYSALQQGVIDGAENST
                     VFYNSQKFNEVAKYYSYTEHFMTPDIPTVSLKWYNAQPKEIQQAIDQAGKELIQNERK
                     YWLEYEKTTADDLKAKGVQFNNADKASIAKMVEPIYKDFEGKIGKDLMDKARAK"
     gene            complement(1141..2709)
                     /locus_tag="VHS06_00090"
     CDS             complement(1141..2709)
                     /locus_tag="VHS06_00090"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX2986566.1"
                     /translation="MVKVVDYMEAAALVSDGSTLWIGGSGGGHSIPEKLIDGLEKRFL
                     ETGKPSRINIITMCGLGDFKETGVGKLAHKGLVNRLISGGFSNCPKMGTLADEGEIEG
                     YTFPMGVISQLSREMAAGRPGLITHVGLHSFVDPRLEGGRQGPRKGPDLVEVIDLAGK
                     EWLFYKPYDVDVTFIRGTTADDKGNISMEKEPIFGDNLSIAMAAHRRGGIVIAQVERL
                     TAAGTIPGKQVKVPCILVDYVVVDPDPWQTYVVKYDPAYAGVIRRPNTEIDPLPLDIR
                     KVIARRAALELRPRAVVNLGFGIANGIALVAAEEGIYEDLTLTVEQGLIGGIPAMGND
                     AGSAVNFEAMIDTPYQFDFYDGGGLDLAFLSFAEVDAKGNVNVSKFRDGVTGPGGFIN
                     IAQNAKKVFFLGTMTAGKSELKLTDRGVQVVQEGSYRKFVPEVRQVTWSGPLAVQSGQ
                     KARFITDRAVFDLTPDGMVLIEVAEGIDIRRDVLEQIPFPILIPREPAKMDPRLFAPE
                     PMSLRSDPAWKESR"
BASE COUNT          607 a         1015 c          826 g          566 t
ORIGIN      
        1 ctgcccctca gcccactgca gcacccgcca aggccacatc ggcacctgca gcttcgtccg
       61 cggcaccgac caatgccgcc gctcctgcaa aagtccagca cgttaagctg agcgaccagg
      121 cggccgagga tcatccggag cagaaggcgt tccgagactt cgccgcccgc gtcaaggagc
      181 tgaccaacgg cgcggtcata gtcgacctct tccccaactc cgtgctcggc agcccgcggg
      241 ggaacatcga gcagatgcag ctcggccagc tcgagttcct caagatctca acggcggccc
      301 tcggcggctt cgtccctcag taccaggtct tcgacctgcc ctacgtcttc cagagccgcg
      361 agcacctgct gtccaagacc gacggtgagg tcgggcagac cctgtccaag ctgctggaga
      421 gcaaactgag catcaaaacg ctgggctact tcgacgccgg cgcccgcagc atcttcaact
      481 ccaagaagcc catcaacact gccgcagacc tcaagggcat gaagatccgc gtcatggaga
      541 gccctctcat gatccagacg ttcaactcga tgggcgcttt ggccactcct atggcagccg
      601 gcgaactgta ctccgcgctg cagcagggcg tcatcgacgg ggccgagaac tccacggtct
      661 tctacaactc ccagaagttc aacgaggtcg cgaagtacta ctcctacacc gagcacttca
      721 tgacccctga catcccgact gtgagcctga agtggtacaa cgcgcagccc aaggagatcc
      781 agcaggcgat cgatcaggcg ggcaaggagc tgatccagaa cgagcgcaag tactggctcg
      841 agtacgagaa gaccacggcc gacgatctga aggccaaggg tgtgcagttc aacaacgccg
      901 acaaggcctc tatcgccaag atggtcgagc cgatctacaa ggacttcgaa ggcaagatcg
      961 gtaaggacct gatggacaag gccagggcca agtaacagca cgcagacata atagagagag
     1021 ggggttgcga gactcgcaac cccctctttg attcctggaa ggctactcag ccgtccagcc
     1081 ccccgtctac cagcgtcgcg atgccatctt caatcgtcta gttgacctgt tcccaggtca
     1141 ctatctcgac tccttccacg caggatcaga cctgagactc atcggctcgg gcgcgaacag
     1201 ccgcggatcc atcttcgcgg gctcgcgagg gatcaggatg gggaagggta tctgttcaag
     1261 gacatccctg cggatgtcga tcccctccgc gacctcgatg agcaccatac cgtctggggt
     1321 tagatcgaag accgctcgat cggtgatgaa gcgcgctttc tggcccgact gaacggcgag
     1381 gggccccgac caagttacct gccgaacctc ggggacgaac ttccggtaac tcccctcctg
     1441 aaccacctgc acgcccctgt ctgtgagctt gagctccgac ttccccgcgg tcatggtgcc
     1501 caggaagaac accttcttcg cgttctgggc gatgttgatg aagccaccgg gtcccgtgac
     1561 gccgtcccgg aacttgctga cgttcacgtt ccctttcgcg tccacctcgg cgaaggatag
     1621 gaaggccagg tccagccccc caccgtcgta gaagtcgaac tggtaggggg tgtcgatcat
     1681 cgcttcgaag ttcacggcgg aaccggcatc gttgcccatg gccgggatcc cgccgatcag
     1741 cccctgctcc accgtgaggg tcagatcctc gtagatcccc tcctcggccg ccacgagggc
     1801 gatgccgttg gctatgccga atcccaggtt taccaccgcc cgtggccgaa gctccagcgc
     1861 cgcgcgccgc gcgatcacct tccgtatatc cagcggcaag gggtcgatct ccgtattggg
     1921 cctgcggatt acgccggcat aggctggatc gtacttcacc acgtaggtct gccagggatc
     1981 ggggtctacc accacgtagt cgaccagaat gcagggcact ttcacctgct tgccgggaat
     2041 ggtgcccgcc gcggtcaatc gctccacctg agcgatcaca atgccgcccc tgcgatgagc
     2101 agccatggct atggagaggt tgtcgccaaa gatcggctcc ttctccatgc tgatgttgcc
     2161 cttgtcatcg gccgtggtgc cgcgaatgaa cgtcacgtcg acgtcgtacg gcttgtagaa
     2221 cagccactcc ttgccggcca gatcgatcac ctcgaccagg tccggacctt tcctggggcc
     2281 ctgccgccca ccctccagcc tggggtcaac gaagctgtga agaccgacgt gggtgatcag
     2341 ccccggtcgt cccgcggcca tctcccggga tagctgcgag atgacaccca tggggaatgt
     2401 gtaaccctcg atctcgccct cgtcggcgag agtgcccatc ttgggacagt tgctgaaccc
     2461 gcccgagatc agcctgttca cgagcccctt gtgggccaac ttgccgacac cggtctcctt
     2521 gaagtcgccc agaccgcaca tcgtgattat gttaatcctg ctgggtttgc cggtctcgag
     2581 gaaccgcttc tccaggccat cgatcaactt ctcaggaatg ctgtggcctc cgcccgaccc
     2641 accgatccag agggtgctgc catccgagac cagagccgcg gcctccatgt agtcaactac
     2701 cttcaccatc aatcggcatt gcctcctctc aacttgtggc aaagcgagga catctggcca
     2761 gtagcgttgg agtgccctta cgaaccccca acgctactgt tcactgcgcc ctggacccgc
     2821 atctgtgtaa tccccactca gcacccagaa cccagcatcc cacaccaact tgggcgctcc
     2881 cgatgcctct acttccagtc tgagctagtt acgccttgtc cctatactcc tggaggatct
     2941 tcctggctat gacagtctat tgaagctgtt gatggtcttc tccacgaagc ggtaggtcac
     3001 aggaaacctc cttc
//
LOCUS       DATALS010000006        12728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_180598, whole genome shotgun sequence.
ACCESSION   DATALS010000006 DATALS010000000
VERSION     DATALS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 12728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12728
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180598"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..818
                     /gene="tmk"
                     /locus_tag="VHS06_00095"
     CDS             204..818
                     /gene="tmk"
                     /locus_tag="VHS06_00095"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX2986567.1"
                     /translation="MITFEGLDGCGKSTQMELLASGLRERGYVVLVTREPGGTPVGES
                     IRQLLLDPRHQGMAAKAEALLYAAARANLVEQVIRPALEDGQVVLCDRYLDSSLAYQG
                     FGRGLGTEDIITLNVWGTGCLFPDLTLFLDLDDPTRVTRLGDVPDRLEAEDEAFHRRV
                     GEGFHELIKMYPHRIRRVDARGTEDEVQKRVRAVVEEEFELFTH"
     gene            821..1957
                     /locus_tag="VHS06_00100"
     CDS             821..1957
                     /locus_tag="VHS06_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEX2986568.1"
                     /translation="MRGVFADIVGQELAVSILTRALEQGPSHAYLFTGPAGVGKTETA
                     LAFAAGLACSEGGCGTCATCKRVQHGLHPDVEIVSAEGNVIRKEEIAEINLHAVYRPF
                     EARAKVYVFPEADSLNVEASNAFLKTLEEPPGHVFFILVTDHPERLLPTVVSRCQSVT
                     FTPVPAGAIVEDLETRLGLPAGEAQLVARVAGGNLRYARELATSNDVRRQRTLLLDAA
                     RDLPQGSLLDIEVALDDVLATVDGRSLEREAQLEAELKQALEWAGDARTRNWLQKRHE
                     EKLKRQQRRLTTQGLQAVTHIFAAWYRDLAAVAVGADQAVLNQDRLSELQEVARPERL
                     AQYTQAVATVRRANERLRYNVDARCAIGDMFRSIKEALTQWPML"
     gene            1942..3321
                     /gene="ricT"
                     /locus_tag="VHS06_00105"
     CDS             1942..3321
                     /gene="ricT"
                     /locus_tag="VHS06_00105"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX2986569.1"
                     /translation="MADVVGVVFQDGNKTYHFDSAGLELSRGDRVVVQTSKGTEIGQV
                     VEPPHFVDDSELPAPLKKVVRVATGKDLESQASGQCLRKEAMATCRELIAQHGLDMKL
                     ISADISFGGERITFNFYSDERVDFRALVGDLAKVLKARVELRQVGAREEARMVGGLGP
                     CGRQLCCCLFPGDEEPVSIRMAKEQNLPLNPVKISGQCGRLMCCLKYEQEQYVKFRKE
                     APPRGTRVSTPTADGVVTGYNVPRDAITVRLEDGTTADLRLSNCEPLAEGGLLFTPDV
                     EEPQPTYTAAEPDYGDRLNAKSGAQTATPEAGDRADSDAAAERGQAAEGAPSGGETGT
                     SKRRRRSRGRRGRGAQAESGQAAAGPAEAGEASTSKRASSGAAKRTGEQAPKEPSKQA
                     GGSRSGHAAAGGQGSGEARPGKDQEAAAEQSDNGERRPRRRRRRRPGSGQASGQAAGE
                     SGSGGGGES"
     gene            complement(3353..3814)
                     /locus_tag="VHS06_00110"
     CDS             complement(3353..3814)
                     /locus_tag="VHS06_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX2986570.1"
                     /translation="METAGLVQTLQTAIGPVVLVSGVGLLLLTMTNRLGRVVDRARLL
                     ARERRGLTGEARDKLEAQLVILGQRARLLRLAIALAAFCVLFAAVLVISLFITALAGA
                     SVPWLIAGLFILSMGSLIAAMVEFIRDINKALQATALEVGEEWEKRWGRPW"
     gene            3967..4039
                     /locus_tag="VHS06_00115"
     tRNA            3967..4039
                     /locus_tag="VHS06_00115"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4000..4002,aa:Thr,seq:cgt)
     gene            4339..5586
                     /locus_tag="VHS06_00120"
     CDS             4339..5586
                     /locus_tag="VHS06_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX2986571.1"
                     /translation="MRSARPAALALLPLLLALMLLTACAPDESTGTADTTTSSSATCT
                     STTFPPADTTSPSAGTETPAAMYRGNPERTGVYPGTGPLEPPELLWKFKTGDVVWSSP
                     VVSQGVVYVASDDDYLYAIDAASGRQKWKFRTGNWIRSSPAVSDGLVYVGSADTCLYA
                     VDAESGEEVGEFPTMAPMFSSPAIQDGTLYFGSDQLYALESGSGQEKWTFKSDEAVRC
                     SPAVSEGVVYVVAGSRVRALTAATGQEIWESENGVQGWGAPYGGELGYCSPAVADGEL
                     YLAGPDSRLFALDAATGRTLWTFSESFAPGSSAAVSAGTVYFCERHGRLFAVDARSGL
                     KKWVFQTGSKEWGGGSVCSSPAVCGGAVYVGGDDGYLYAVDAQTGKGLWKYQTGGMVR
                     SSPAVSDGVVYFGSADGYLYALK"
     gene            5790..6344
                     /locus_tag="VHS06_00125"
     CDS             5790..6344
                     /locus_tag="VHS06_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2986572.1"
                     /translation="MPGHDERANSDVFFKLFILVAQTRDALLKARERDYARCGINSDR
                     RAILWLIENNGGRATPVEIARDLFRELNSVSEMLKRMERDGLITRHKGTGRSKTEVRI
                     TPKGLDIFNQSYHNETTQKIISALTLEERDLLESLLGKLRSRTLEELGIAEWQIRFPL
                     EAEPAERDAVAVDSALSAGLSAEA"
     gene            7206..9839
                     /locus_tag="VHS06_00130"
     CDS             7206..9839
                     /locus_tag="VHS06_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2986573.1"
                     /translation="MAESEKRTTVDKSVIKALALGGTQGGGAPCVVDVKNGKAVRVRP
                     LHYDWKYDLDTINQWKLERNGKVLRPIIKSVPSPFSLAYKKRVYSPNRIKYPLKRVDW
                     DPSGERNTQTRGSAKYKRISWDEATDIIAAEIKRVHKEYGPYAVLTQTDGHSECKHLN
                     PPHGACTLLLSKLGGYTQQVRNADSWEGWYWGSKHVWGDGNVGMMSPAQNVAKDIIEN
                     CDMVICWGGDLETTPWGFGGQQGTFMSYYLTEAGIPQIFVCPDLNYACAIHADKWIPV
                     LPNTDAAVQLAVIYTWIKEGTYDKDYVATHTVGFDQFKAYVMGEEDGIEKSPEWAAPK
                     SGVPEWTIKALARQFAAKCTSIIHYFGGGMIRGPWSTEPARLESIMLGMQGLGGPGVH
                     QAQLTLTGMPRSDAPATTMRLNQEGSPPGPLPTQPRLPELHFMTVNCYAKQNIPKTMI
                     EEAILNPPLTYWGHGSIRVKVEDQFVKYTYPIPKEEGGSEIHMIWTDSPCRTTCWGDG
                     MRTVEAMRHPKIEFILAQHPWMENDCFFADIILPVNTTFEVEDLVPNTRPGIEMLSAG
                     LQHKAIEPIGESMSDYEAVLEVAKKLDMYDEVTEGKTMEEWKRHLFDDYFKLNKYTTW
                     EEFNEKEYMIFPAAEDWEEDPPGFRLFYEDPESYPLPTPSGKLEFYSQRLADNFPEDV
                     ERPPIPKWVEKGPANDERLTSPRARMFPLLAMSNHGRWRMHAQCDDITWDREVGTCKR
                     RGNDGYLYEPLWMHPQTAEERGIKDGDIVKAYNERGTVLFVAYVWERIIPGAVYADHG
                     SRVDWIIPGKLDRGGAINLIGSVLTSKHAGGQSTTGYLVEVAKVTGEEMDQWRRDYPE
                     AFERAYDPAAGLRFDAWVEGE"
     gene            9843..10652
                     /locus_tag="VHS06_00135"
     CDS             9843..10652
                     /locus_tag="VHS06_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2986574.1"
                     /translation="MKAFAVDVSRCNGCYCCQIACKDEHVANDWTPYAKPQPDTGQFW
                     CKLNEYVQGQVPKVKMHYVPVMCQHCDNAACIEACKVEGAVYKREDGLVVIDPEKCTG
                     CKNCADSCAYNAIYFNEDMNIAQKCTGCAHLLDDGWKEPRCVDACPTGALKFGEEAEM
                     AALAGQKTPLRPELDTQPRVYYYDIPKQFVGGTVYDPVQKEVVIGATVTINAAGKTMT
                     TTTDEFGDFWFHGLDAGSRSISIEAKGFKPKTIDAINTEESVSLGDIPLEA"
     gene            complement(10641..12611)
                     /locus_tag="VHS06_00140"
     CDS             complement(10641..12611)
                     /locus_tag="VHS06_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX2986575.1"
                     /translation="MFKACIDTGGTFTDCVVRDGEGKMREFKPPSTPPDFSTGVVNSL
                     REAAQGYGLSLEDFLKDTETIVHGTTAATNALVTKNGARTALITTEGFRDIIEMGRSL
                     KIDTHSMYDGYIPPYEPLVPRYLRFTVKEKTKSTGEIIKPVSEDELLEVIEKIKQEKI
                     EALAICFINCYVNPENELKAADFCAKHLPDVFVTASCKILPKMGEYERESTCVISAYL
                     GPVVNRYLSNLDEELKRQGFGGQLLIMQSNTYAQSVRAVTEKPAYLMGSGPAAAPAGA
                     AFLGTYTNEPNLITADVGGTTFDAGQVRTGNVSLVSGQWLGEDRLGLKVVDVTSLGAG
                     GGSIGWIDSLGLLKVGPQSAGADPGPACYGHGNMEPTITDACLLLGYMSDDYFWGGKL
                     PLHKDLAREAMRKVSEPLKLSVEEAAMAMIITCSSNMADGTTEITTRQGYDIRDYSLL
                     AIGGGGGWSGAYVANILGMKRVIVPRFSASFSAWSMFCLDLGRDYLRPYVSMVNFANL
                     DTINRLYGHMINEAKDEFKALHVGPADIIFEKSMDLCYKGQYHDLEVSLGQVDWITQE
                     TLDQIDKDFHQKHKELFTFNLPYVPIVMKNLRLIAKVQTQKPSLSKVPAGQAPWNGAA
                     VAQAPIGDPSAALKRERLAYWDGRFMNTPLGF"
BASE COUNT         2480 a         3992 c         3990 g         2266 t
ORIGIN      
        1 ccacgccggc cggcccggta aagaggtagg cgtgcgaagg cccctgctcg agcgcccgag
       61 taaggatcga gacagccaac tcctgccccc ccccagagga gcctcggtcg gataggggcg
      121 ggtgggacta tccgctgcct cccgaggacg ccagccttga cgactcggta ctggccgaat
      181 tggggagtgg gctggagggt ctgttgatca ccttcgaagg cctggacggc tgcggtaaat
      241 ccacccagat ggaactcctg gccagcggtt tgcgcgaacg gggctacgtg gtcctagtca
      301 cgcgggagcc gggcgggacc cccgtgggcg aatccatccg gcagctcttg ctcgaccctc
      361 gccaccaggg catggccgct aaggcggaag cacttctcta tgctgcggcg cgggccaacc
      421 tggttgaaca ggtcatccgg ccggcgctgg aggacggaca ggtggtcctg tgtgaccgct
      481 acctcgactc gtctcttgcc tatcaaggct ttggccgggg cctgggcacc gaagacatca
      541 tcaccctgaa tgtatggggc accggctgtc tcttcccgga cctcacgctg ttcctcgatc
      601 ttgacgaccc cacgcgtgtc acccgcttgg gagacgtgcc cgatcgcctg gaggccgaag
      661 acgaggcgtt tcaccgccgg gtaggggagg gctttcacga gctgatcaag atgtacccgc
      721 atcgcattcg gcgggtcgac gcccgcggca ccgaggacga ggtgcagaag cgggtgcggg
      781 ccgtggtgga ggaagagttc gaactcttca cgcactagcg atgaggggcg tcttcgccga
      841 cattgtgggg caggagttgg ctgtctcgat ccttactcgg gcgctcgagc aggggccttc
      901 gcacgcctac ctctttaccg ggccggccgg cgtgggcaag acggagacgg cgctggcctt
      961 cgcggcgggg ctcgcttgca gcgaaggcgg gtgcgggacg tgtgccacgt gtaaacgggt
     1021 gcagcacggc cttcatccgg atgtggagat cgtctcggcg gaaggcaacg tcatccgcaa
     1081 agaggaaatc gcggagatca acctgcatgc ggtctatcgc cccttcgagg ctcgcgccaa
     1141 agtgtacgtg tttccggagg ccgacagcct gaatgtcgag gcgtcgaacg cttttctgaa
     1201 gacgctcgag gaaccgcccg ggcacgtttt cttcatcctc gtgaccgacc atcccgagcg
     1261 cctgctgccg accgtggtat ctcggtgcca gtccgtcacc ttcacgccgg tccccgcagg
     1321 agcgatcgtg gaagatctgg agaccaggct gggactgccg gccggggagg cgcaactggt
     1381 cgcgcgggta gccgggggca acctgcgcta cgcgcgggaa ttagctacgt ccaacgacgt
     1441 ccggcggcag cgcacgttgc tcctagacgc ggcccgcgat ctaccccagg gcagcttgtt
     1501 ggatatagag gtagctctcg atgacgtcct agccacggtc gacggccgct cgcttgaaag
     1561 agaagcccaa ctggaggccg agctcaagca agcgctggaa tgggcgggag acgcgcggac
     1621 gcgcaactgg cttcagaagc gccacgagga aaagctgaag cgccagcagc ggcgtttgac
     1681 cacccagggg ctgcaggcag tcacacacat ctttgctgct tggtaccggg acctcgccgc
     1741 ggtggccgtg ggcgcggacc aggctgtgct caaccaggac cgcctgagcg agctgcagga
     1801 ggtcgctcgg ccggagcgcc tcgcccagta cacgcaggcg gtggccactg tacggcgggc
     1861 gaatgagagg ctccgctata atgtcgatgc ccgatgcgcc atcggggaca tgtttcgaag
     1921 tattaaggag gccctgactc agtggccgat gttgtagggg tcgtgttcca ggatgggaac
     1981 aagacctacc atttcgactc cgccgggttg gaactgtccc ggggagaccg cgtagtggta
     2041 cagacgagca aagggacgga gatcggccag gtggtcgaac ccccgcactt tgtcgacgat
     2101 tccgaattgc ccgctcctct caagaaggtg gtgcgggtgg ctaccggtaa ggatctcgag
     2161 tcgcaagcca gcggtcagtg cctacgtaag gaagctatgg ccacctgccg cgaactcatc
     2221 gcgcagcacg gcttggacat gaagctcatc agtgccgata tctccttcgg cggtgaacgc
     2281 atcacgttca acttctacag cgatgagcgc gtcgatttcc gcgcgcttgt cggcgacctg
     2341 gccaaggtgc tgaaggcacg ggtggaactg aggcaggtcg gggcgcgcga ggaagcgcgc
     2401 atggtgggag gcctcgggcc ctgtggccgg cagctttgct gctgcctctt cccgggggac
     2461 gaggaaccgg tctccatccg catggccaag gagcagaatc tgcccctcaa ccccgtcaag
     2521 atatccgggc aatgtggccg cctgatgtgc tgtctgaagt acgagcagga acagtacgtc
     2581 aagtttcgca aggaagctcc gccccggggg acacgcgtca gcacgcccac ggccgacggg
     2641 gtggtcacgg gctacaacgt ccctcgcgac gcgatcaccg tccgcctgga agacggtacg
     2701 accgccgacc ttcgtctcag caactgcgag ccgcttgcag agggcggcct tctgttcacg
     2761 cctgacgtgg aggaaccgca gccgacgtat acggccgctg aacccgacta cggggacagg
     2821 ctgaacgcca agagcggggc acagactgcg acgcccgaag ccggtgaccg tgctgacagc
     2881 gacgcggctg ctgagcgggg ccaagctgcc gagggggcgc cttctggcgg agagaccggc
     2941 acgtccaagc gccgccgccg cagccgcggc cgccgaggac gcggcgcgca ggccgagagc
     3001 ggtcaggcgg ccgccggtcc tgcggaagcg ggtgaggcct cgacctcgaa gcgagcctcg
     3061 tcgggggccg ctaagcgtac gggagaacaa gcacccaagg aaccgtccaa acaggcgggt
     3121 ggatcgcggt ctggccacgc ggcggccgga ggccaaggct cgggtgaagc acgacccggc
     3181 aaagatcaag aggcggctgc ggagcagtcc gataacggcg aacgccgtcc gcgccgccgt
     3241 cgtcggcggc gaccgggcag cggccaggct tctggtcagg ctgccggtga gagcggtagt
     3301 ggtggaggcg gcgagagcta agtggggcgg accgccgtgg cccgccgcat cctcaccacg
     3361 gcctacccca gcgcttctcc cactcttccc ccacctcgag ggcggtcgcc tgtaacgcct
     3421 tgttgatgtc gcggatgaac tccaccatcg cggcgatgag actccccatg ctcaggatga
     3481 agagccctgc gatcagccag ggcacgctgg cgccggccag cgccgtgatg aagagactga
     3541 tcacaaggac ggcggcgaag agcacgcaga aggcggcgag agcaatcgcc agccgaagaa
     3601 gtcgggcgcg ttgccccaag atgacgagtt gtgcctccaa cttgtctctc gcctccccgg
     3661 tcaacccccg tcgctcgcgc gccagcaagc gggcgcgatc gaccacgcgg cccagccggt
     3721 tagtcatggt gagcagcagt agcccgacac cggacacaag taccaccgga ccgatggcgg
     3781 tctgcagggt ctgcacaagt ccagctgttt ccatcccggg cctttcagcg ggaggcaccc
     3841 ggcgatgcct tcgaacagga tgttgctcgg gcggaggctt ctcaaacccc gagcccccga
     3901 gagtgtgcct ttcggtgctc cgtgcttgtc aggcagaggc tgagcttcta aactaacgct
     3961 tcgcgcgccg gcgtagctca gatggcagag cggctcactc gtaatgagca ggtccggggt
     4021 tcgattcccc gcgccggctt ccttcgcgcc ctccaccttc ccactttcgc agcactcatc
     4081 gtcgctcgcc tctcagcgct cgctgcactc cgatacccga tatccacttt acaaagagcg
     4141 ataagtggat atatacttta gacacagtga agctgtgcct gagcaacccc cccccgttca
     4201 ggctgaaggc agagtgaagc ggtctgggtt atccccccct cccaggccgc ttttgccttt
     4261 tcaggagtcg cttgcttagg agtcatctgc tctacactgg tgttgtaagg acagccggac
     4321 ctctctgagg aggtggccat gaggtccgct cgacctgccg cgcttgccct cctccccctt
     4381 ctgcttgccc tcatgctgct gaccgcgtgc gccccggatg agagcaccgg caccgctgac
     4441 acaaccacct cttcgtccgc aacctgcacc agcaccacct ttccccctgc cgacacgacc
     4501 agcccttccg ctgggacgga aacaccagcc gcgatgtacc gggggaatcc tgaacggact
     4561 ggggtttacc cgggtactgg cccgcttgag ccacccgagt tgctgtggaa gttcaagaca
     4621 ggcgacgtcg tctggtcgtc accggtggtc agccagggag tcgtctacgt cgccagtgac
     4681 gacgactatc tctacgcgat cgacgcggca agcggcaggc agaaatggaa gttccgaacg
     4741 ggtaactgga ttcgatcctc cccggcggta tcggacggct tggtttacgt cggaagtgcc
     4801 gatacctgcc tctatgcggt ggatgccgaa agcggtgaag aggtgggtga gtttccgacc
     4861 atggcgccca tgttctcgtc tccagcgata caggacggga ccctctattt cgggagcgac
     4921 cagctgtatg ccctcgagag tgggagcggg caggagaaat ggacgttcaa gagcgatgag
     4981 gcagtgcgtt gctctccggc cgtctcggaa ggcgtcgtgt atgttgtcgc cggctcccgg
     5041 gttcgcgcgt tgacggccgc caccggtcag gagatttggg agtccgaaaa cggcgtgcag
     5101 ggttggggtg ccccttatgg tggcgaactc ggctactgct ccccggccgt ggccgacggc
     5161 gagctctatc tggccggccc cgacagccgg ctcttcgcgc tcgacgccgc gaccggccgc
     5221 acgctgtgga ccttctcgga atccttcgcc ccggggtcct ccgcggcggt gtcggccggg
     5281 accgtctact tctgcgagcg gcatggtcgc ctctttgcgg ttgatgcccg gagtggcctg
     5341 aagaaatggg tcttccagac cggctccaag gaatggggcg gcggcagcgt ctgctcttcg
     5401 ccggccgtct gcgggggcgc ggtgtacgtg ggcggagatg acggctacct gtatgcagtg
     5461 gacgcccaga ccggcaaggg gctatggaaa tatcaaacgg ggggtatggt gcggtcctcc
     5521 ccggccgtct cagacggcgt ggtctacttc ggcagcgccg acggctatct ctacgccctc
     5581 aaataggacg atcctcgcgc cacttcagaa ttatgtgcgg atacgaacgt cgccgtgcta
     5641 tcttcatgag cacggagggg tcccgcaggt tcaagaccgg tgggggcggt tgggtccgtc
     5701 cggaaacgga gaggttgctg gggccgctct gtcgctgaat tcctcccctc gccggcgtga
     5761 agggtcatcg gctaaggacg ggggaacaga tgccgggtca cgatgaacgg gccaattccg
     5821 acgttttttt caagcttttt atcctcgttg cccaaaccag ggacgctctg ctgaaagcac
     5881 gcgagaggga ctacgcgcgt tgcggaatca acagcgatag gcgcgccatt ctgtggctca
     5941 tcgagaacaa cggcggacgc gcgacgcctg tcgagatcgc ccgcgacctc ttccgcgaac
     6001 tgaactcggt cagcgagatg ctgaagcgga tggaacgaga cggcctgata accaggcaca
     6061 agggtacggg acggtcgaag accgaggtac gcatcacccc caagggcttg gacatcttca
     6121 accagtccta tcacaacgag accacacaga agatcatctc cgcgctcacc ctggaagagc
     6181 gagacctgct cgaatcgttg ctcggaaaac tacgcagccg gaccttggag gaactcggca
     6241 tagccgaatg gcagatccgc tttccgctgg aggctgagcc tgccgagcgc gacgcagtgg
     6301 cggtcgattc tgccctgtca gccggtcttt ctgctgaggc atagaccccg aatgagagtg
     6361 ttcgttcgca aaaaagttgc atcctcgcgg gggtttttcc tcctctggcc tacttttccc
     6421 tattgacttt ggtccggccc atgcgatagg gtgaggtcct cattcgtata cgaatgtgag
     6481 cgtgtagcgc agatcgtcgt gttctagttg gggggacgcg cgaatgggca aggctttggc
     6541 gacctataag gttcgcggca cgaggccctt ccatgaggaa tccggaagga cgccgtcctc
     6601 gaatgcagac ccgctccggt ggtcgtctca gtctgcgccg tcactcctgc gtcttggttc
     6661 ggatcattcg cgatccactt tcccaagttt catcctcgag gactgtcagt ccctatccgc
     6721 gcctggtcgg cggccgacta tccgccgttg ccaggcctga cagccgtgag ttcatcaaca
     6781 tcccagacaa gcgtccaaac cgtggaggtg ataccgagct aactaaatcg caggagccga
     6841 acgcaatcgc ttagcaggat ttgtctcatt acgaccgggg ggaactgtag tgaagaagcg
     6901 aaaccaacta gccggggcac cggcaccgag gatgaggtcc ctctcacact cggcgcagat
     6961 catgcccagt tggccctccc ccgtcacctc catactcacc ccctccgccc atccccgtga
     7021 tgggcatccg gaggaagtgc gaccgcatct tggtcgctca catccagaca aaccgcgtcc
     7081 ccatagtaat caggggagtc tccagccgag gagtcggcgc agaagggggg caacaggtca
     7141 cgtactccca tagatactta gttcgcttat taccatttcg ctggtaaatg gcaaaggaga
     7201 ggaagatggc agagtccgaa aaacgtacga cagtagataa gtcagtcatc aaggcacttg
     7261 cccttggcgg cacgcagggc ggaggagcgc cgtgtgtggt cgacgtcaag aacggcaagg
     7321 cggtgcgggt caggccgctg cactacgact ggaaatacga tctcgacacg atcaaccagt
     7381 ggaagctgga gagaaacggg aaggtgctca ggccgatcat caaatcggtt ccctccccgt
     7441 tctccttggc ttacaagaag cgggtctatt ctcccaaccg catcaagtac cccctgaaaa
     7501 gggtcgactg ggatccgagc ggcgagcgga acacccagac ccgcggttcg gccaagtaca
     7561 agcggatctc ttgggacgag gcgaccgaca tcatcgcggc cgagatcaaa cgggtccata
     7621 aggaatacgg cccgtacgcc gtccttaccc agaccgacgg ccactcagaa tgcaagcacc
     7681 tcaacccgcc gcacggcgcc tgcaccctgc tcctgagcaa gctgggcggc tacacccagc
     7741 aggtccgcaa cgccgactcc tgggaaggtt ggtactgggg ttccaagcac gtctggggcg
     7801 acggtaacgt cggcatgatg agcccggccc agaacgtggc caaggatatc atcgagaact
     7861 gcgacatggt catctgctgg ggtggcgacc tcgagaccac gccctggggc ttcggtggcc
     7921 agcagggcac cttcatgagc tactacctga ccgaagccgg tatcccgcag atcttcgtct
     7981 gcccggacct caactacgcc tgcgccatcc acgccgataa gtggatcccg gtgctgccga
     8041 acaccgatgc ggctgtgcag ctggccgtca tctacacctg gatcaaagag ggcacctacg
     8101 acaaggacta cgtcgcgacc cacacggtcg gcttcgacca gttcaaagcg tacgtcatgg
     8161 gtgaggaaga cgggatcgag aagagtcccg agtgggcggc tcccaaatcg ggcgttcccg
     8221 aatggactat caaagcgctt gcccgccagt tcgcggcgaa gtgcacttcc atcatccact
     8281 acttcggcgg cggcatgatc cgcggcccgt ggtcgaccga gccggctcgt ctcgaatcga
     8341 tcatgttggg catgcaaggc ctgggaggcc ccggcgtcca tcaggcgcag ctcaccctga
     8401 cgggtatgcc gcgcagcgac gctccggcca ccaccatgcg cctcaaccag gaaggttcgc
     8461 ctccaggacc gctgcccact cagccgcgtc tgccggaact ccacttcatg acggtgaact
     8521 gctacgccaa gcagaacatc cccaaaacca tgatcgagga ggccatcctc aacccgccgc
     8581 tcacctactg gggccatggc tccatccggg tgaaggtgga ggaccagttc gtcaagtaca
     8641 cctacccgat ccccaaagaa gagggcggct cggagatcca catgatctgg accgactctc
     8701 cctgccgcac cacttgctgg ggcgacggca tgcgcacggt cgaggccatg cgccatccca
     8761 agatcgagtt catcctcgcg cagcatccct ggatggagaa cgactgcttc ttcgccgaca
     8821 tcatcctccc ggtcaacacc accttcgagg tggaagacct ggtcccgaac acccggccgg
     8881 gcatcgagat gctgagcgcc ggcctccagc acaaggccat cgagcccatc ggtgaatcca
     8941 tgagcgacta cgaggccgtc ctggaagtcg ccaagaagct cgacatgtac gacgaggtca
     9001 ccgaaggcaa gacgatggaa gagtggaagc gccatctctt cgatgactac ttcaagctga
     9061 acaagtacac cacctgggaa gagttcaacg agaaggaata catgatcttc cccgccgccg
     9121 aggactggga agaagatcct ccgggcttcc ggctcttcta tgaggacccc gagagctacc
     9181 cgctgccgac gccgtcgggc aagctggagt tctactccca gcgcctggcc gacaacttcc
     9241 cggaagacgt cgagcggccg ccgatcccca aatgggtcga aaagggcccg gccaacgacg
     9301 agcgtctcac cagcccccgg gcgcggatgt tcccgctgct tgccatgagc aaccacggtc
     9361 gctggcgtat gcacgcccag tgcgacgaca tcacctggga tcgcgaagtg ggcacgtgta
     9421 agcggcgcgg taacgacggc tacctctacg agccgctgtg gatgcatccg cagacggccg
     9481 aagagcgggg catcaaagac ggcgacatcg tcaaggccta caacgaacgc ggcacggtcc
     9541 tcttcgtggc ctacgtctgg gaacgcatca tcccgggcgc cgtctacgcg gaccacggct
     9601 cgcgggtcga ctggatcatc cccggcaagc tcgaccgcgg tggcgccatc aacctcatcg
     9661 gtagcgtcct tacctccaag cacgccggcg gccagtccac cactggctac ctggtcgagg
     9721 tcgccaaagt gaccggcgaa gagatggacc agtggaggag agattacccc gaggccttcg
     9781 agcgcgccta cgacccagca gccggcctac gcttcgacgc ttgggtggaa ggagagtaac
     9841 tgatgaaagc tttcgcggtc gacgtttcca ggtgtaacgg ctgctactgc tgccagatcg
     9901 cctgcaaaga cgagcacgtg gccaacgact ggaccccgta tgccaaacca cagccggata
     9961 ccggccagtt ctggtgcaag ctgaacgaat atgtccaggg ccaggtgccc aaggtcaaga
    10021 tgcactacgt gccggtcatg tgccagcact gcgacaacgc ggcctgcatc gaggcctgca
    10081 aggtggaagg cgccgtctac aaacgggaag acggcctggt ggtcatcgat cccgagaagt
    10141 gcacgggctg caagaactgc gccgactcct gcgcctacaa cgccatctac ttcaacgaag
    10201 atatgaacat cgcccagaag tgcaccggtt gtgcgcatct cctcgacgac ggctggaaag
    10261 agccgcgctg cgtggacgcc tgcccgaccg gcgcgctcaa gttcggcgaa gaggccgaga
    10321 tggccgccct cgcgggtcag aagactccgc tgcggcccga actcgacacc cagccgcggg
    10381 tctactacta cgacatcccg aagcagttcg tgggtggaac ggtgtacgac ccggtacaga
    10441 aggaagtcgt tatcggcgcc acggtcacta tcaacgcggc cggcaagacc atgacgacca
    10501 ccacggacga gttcggcgac ttctggttcc acggactgga cgccggctct cgttccatca
    10561 gcatcgaggc caaagggttc aagcccaaaa ccatagacgc catcaacact gaggagagcg
    10621 tcagtctggg tgacatacct ctagaagcct aacggcgtgt tcatgaagcg accatcccaa
    10681 taggccagcc gctctctctt gagagcagcc gagggatcac cgatcggggc ttgcgccacc
    10741 gcggcaccat tccacggcgc ctggccggcc ggtaccttgg aaagactcgg tttctgcgtc
    10801 tgcactttgg cgatgaggcg caggttcttc atcacgatag gcacgtaggg aagattgaac
    10861 gtgaacagct ctttgtgctt ctggtggaag tctttgtcga tctggtccaa ggtctcctgg
    10921 gtgatccagt cgacctggcc cagagagacc tcgaggtcgt gatactgacc tttgtagcaa
    10981 aggtccatcg acttctcgaa gatgatgtcg gcaggtccga cgtgaagcgc tttgaactcg
    11041 tccttggcct cgttgatcat gtggccgtac agccggttga tcgtgtccag gttggcgaaa
    11101 ttcaccatgc tcacgtatgg gcgcagatag tcgcggccga ggtcgagaca gaacatgctc
    11161 caggcactga aggaggccga gaaccgagga acgatcacgc gtttcatgcc gaggatgttg
    11221 gcgacatacg cgcccgacca tccaccgccg ccgccgatgg cgagcaggct gtagtcacgg
    11281 atgtcatagc cctggcgggt cgtgatctcc gttgtaccgt cggccatgtt ggagctgcag
    11341 gtgatgatca tggccatggc cgcctcttcc acgctcagct tcagaggttc ggagaccttc
    11401 cgcatagcct ctctggccag gtctttgtgc agcgggagtt ttcctcccca gaagtaatcg
    11461 tccgacatgt atccgagaag gagacaagcg tcggtgatcg tcggctccat gttgccgtgg
    11521 ccgtaacacg ccggaccggg atccgcgccg gcactctgcg gtcccacctt gagcagaccg
    11581 agtgagtcga tccaaccgat gctgccgccg ccggcgccca ggctggtgac gtcgaccact
    11641 ttgaggccca ggcgatcttc gccgagccac tggccggaga ccaggctcac gttgcccgtg
    11701 cgcacctggc cggcgtcgaa ggtcgtgcca ccgacgtccg ccgtgatcag gttcggttcg
    11761 ttggtgtacg tgcccaagaa ggcagcgccg gccggagcag ccgccgggcc cgagcccatg
    11821 aggtaggccg gtttctcggt gaccgctcgg actgactggg cgtaggtgtt ggactgcatg
    11881 atgagcaact ggccgccgaa gccctgccgc ttcaactcct catccagatt ggagaggtag
    11941 cggttgacca cgggtcccag gtaggcgctg atcacacagg tgctttcccg ctcatactcg
    12001 cccatcttgg gcagaatctt gcaggaggcg gtgacaaaga cgtccggaag gtgcttcgcg
    12061 cagaaatccg ccgccttcag ttcgttttcg ggattcacgt agcagttgat gaagcagatg
    12121 gctaaggcct cgatcttctc ctgctttatc ttctcgatga cctcgagcag ttcgtcctcg
    12181 ctcaccggct tgatgatttc gccggtggac ttagtttttt cctttacggt gaagcggaga
    12241 taccgcggta ccagtggttc gtaaggagga atgtatccgt catacatgga gtgggtatcg
    12301 atcttgagcg accgacccat ctcgatgatg tccctgaacc cttcggtggt aatgagggcg
    12361 gtgcgtgccc cattctttgt caccagagcg tttgtggccg ccgtagtgcc gtgcacgatg
    12421 gtctcggtgt ccttgaggaa atcttccagg ctcaggccat aaccctgagc ggcctccctc
    12481 aacgagttca ccacgccggt cgagaagtcc ggaggcgtgc ttggcggctt gaactccctc
    12541 atcttgcctt caccatcccg cacgacgcag tccgtgaagg tcccccccgt atcgatacat
    12601 gctttaaaca taagctcccc ttttgatcgt cccgcaacag tcagtaagac gagctactag
    12661 cctcggctac tggcgctcaa ctagttttcc cccccccgcc ccctttctac ggcttaggcc
    12721 ggccgggc
//
LOCUS       DATALS010000007         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_361002, whole genome shotgun sequence.
ACCESSION   DATALS010000007 DATALS010000000
VERSION     DATALS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361002"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VHS06_00145"
     CDS             <1..443
                     /locus_tag="VHS06_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986576.1"
                     /translation="LELGITRYFEVALFQGFSPVESVLAFELGLVTKKPVLVSAGMLG
                     LQNGAKAQPFLEGGYYRGKTILIAGVQGQNSAVYGIFGAAYQATQRLLLAADYISGSP
                     NFATLGVTYAITPNLSLNPAVYISNSTPHHAYGYAVLTWNVKAW"
     gene            complement(522..1202)
                     /locus_tag="VHS06_00150"
     CDS             complement(522..1202)
                     /locus_tag="VHS06_00150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX2986577.1"
                     /translation="MSISTDSAGPTCPPEPTRFLVFSASLRTGSYNTRLAQLAVRTIE
                     QRGATVDMASMAAFDCPSFNQDIEDTKGFPPGAEEFRSRIEANDALVIASPEYNGSMP
                     GLLKNAIDWVSRYDPQPFDQRHVLLMSASPSMAGGNRGLWSLRIPLEHLGARVFPDMF
                     SLATANQALNDEGQIINPVLSHRFAGNVEAFMNLVEAAKFYPRLKKAWIEFLGEQPNP
                     ALEHAGRA"
     gene            1393..1776
                     /locus_tag="VHS06_00155"
     CDS             1393..1776
                     /locus_tag="VHS06_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HEX2986578.1"
                     /translation="MEDNRMPTQGQLQADISEAIIKFEKEYMGRGPLETKTYIVEDLV
                     IARLRGVLTQAEHQLARTSESSRGRDLIKQMRVELIEKGRPLLEAVVESLTGRRVQSL
                     HTDISTSTGERLIVFTLDGPPIIDQ"
     gene            complement(1717..2364)
                     /locus_tag="VHS06_00160"
     CDS             complement(1717..2364)
                     /locus_tag="VHS06_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986579.1"
                     /translation="MIDNRLLTPLRVAAAIAILLCMIGSEGLAQRERGRFPGQAPSVG
                     ATDSTSSRPAPRERRPHGNVAPPDVPEATPPARKGPLDVNTLRYALIDARTRAVTFIG
                     YYDPAYATGPIPYADILKDTLDNPYPSFSIEPTQQQAQLERVDKMITADIARMSDPEY
                     CNQWGQKLVNLLLNDASLPADSERFYAWAFVNSIERSLVDDRRPVEREDYQSLTC"
BASE COUNT          496 a          729 c          761 g          493 t
ORIGIN      
        1 agctcgaact cgggatcacc cgatactttg aggtggcgct cttccagggc ttctcaccgg
       61 tggagtcggt gttagcattt gagctggggt tggttacgaa gaagccggtt ctggtctcgg
      121 ccggaatgct ggggctgcag aacggggcga aggctcagcc gttccttgag ggcggatact
      181 atcgtggaaa gaccatcctc atcgccggcg ttcagggtca aaactccgcg gtctacggta
      241 tcttcggagc ggcctaccag gccacccagc gcctcctgct ggccgccgac tacatcagcg
      301 gatcgcccaa cttcgccacg ctcggggtga cttatgccat cacccccaac ctctccctca
      361 accccgccgt ctacatctcc aacagcaccc ctcaccacgc ctacggctac gccgtattga
      421 cctggaacgt gaaggcctgg tagccacgtc caggatgctc ggttcccgtc tcgatgcgcc
      481 gcaccgagtc cacgatagac gccagccttc caaggccggc atcaggctcg ccccgcgtgc
      541 tccagtgccg ggttgggctg ttctccgagg aactcgatcc aagccttctt gaggcgtggg
      601 tagaactttg cggcctcgac gaggttcatg aaggcctcga cgttgcctgc aaacctatgg
      661 ctaagtaccg ggttgatgat ctggccttcg tcattcaagg cctggttggc ggtggcgagt
      721 gagaacatgt cggggaagac cctcgccccc aggtgctcaa ggggaatgcg cagcgaccac
      781 agtccgcggt ttcctccggc catagagggt gaggccgaca tcaagagcac gtgacgctga
      841 tcgaagggct gcggatcgta gcgggacacc cagtctatag cgttcttcag cagcccgggc
      901 atagagccgt tatactcggg cgaggcgatg accagagcat cattcgcctc gatccgactg
      961 cggaactcct ccgcacctgg aggaaacccc ttggtatcct cgatatcctg attgaaggac
     1021 gggcagtcga aggccgccat tgaggccatg tccacggtcg cgccgcgctg ctcgatcgtg
     1081 cgcaccgcca actgcgccag ccgcgtgttg taagagccgg tccgcagaga tgcggagaac
     1141 accaggaaac gcgttggttc gggcgggcac gtagggcccg cactatcggt cgatatggac
     1201 atggtttgct cctcctgtct cgatgtgagt attctgagat cagtcagagt aggttaccca
     1261 gtcttgcttc cgttgtctca ccgcacggtg ctagctgagt tcctgtgaac ccaacggttg
     1321 ggggtgtgca ggaagatgac cgtccggcgc agtagagctt aagtgactga gcgacagctc
     1381 aggggatatg cagtggagga caacaggatg ccgacgcagg gacagctcca ggccgacatc
     1441 agtgaagcta tcataaagtt cgaaaaggag tacatggggc gaggtccgct cgagaccaag
     1501 acgtacatag tggaggatct cgtgatcgcc aggctcagag gggttctcac gcaggcggag
     1561 caccaactgg cgaggacgag tgaatcatcg agggggcgcg accttatcaa gcagatgcgg
     1621 gtcgagctga tcgagaaggg acggccgctc ctcgaggccg ttgtggagtc cctcacaggt
     1681 cgcagggtgc agagcctgca caccgatatc agcacctcaa caggtgagcg actgatagtc
     1741 ttcacgctcg acgggccgcc gatcatcgac cagtgacctc tcgatactgt tgacaaacgc
     1801 ccacgcatag aaacgctcgc tatccgccgg cagcgatgca tcgttcagca gcagattcac
     1861 cagcttctgc ccccactgat tgcagtattc agggtcgctc attctggcta tatcagccgt
     1921 tatcatcttg tctacccgct cgagctgcgc ctgctgctgg gtcggctcga tcgagaatga
     1981 gggatagggg ttgtccaatg tgtccttcag aatatcggcg taagggatcg gtccggtagc
     2041 ataggccgga tcataatagc cgatgaaggt gacagcgcgg gtcctggcgt cgatcagcgc
     2101 gtatctgagc gtgttgacat cgagtggccc cttcctggcc ggaggagtcg cctcgggaac
     2161 gtcgggcggt gcgacgttcc cgtgtggacg ccgttccctc ggtgccggcc gagaactggt
     2221 tgagtccgtg gcaccgacac tgggagcctg tccagggaat ctacctctct ctcgctgtgc
     2281 cagaccttca ctgcctatca tgcacaggag gatggcgata gcagctgcga cgcgcagcgg
     2341 ggtcagaagc ctgttgtcaa tcacggttag ctacctctgt tggagttcgc ggtatcaaca
     2401 tgatccatta tggcctcgcg cgcaccaaaa gcgaggtagg caacccctat cgactgcccc
     2461 cttgatatgg agtacgaag
//
LOCUS       DATALS010000008         4172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_180945, whole genome shotgun sequence.
ACCESSION   DATALS010000008 DATALS010000000
VERSION     DATALS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180945"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VHS06_00165"
     CDS             complement(<1..619)
                     /locus_tag="VHS06_00165"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX2986580.1"
                     /translation="MSLLETEPKATTGAAAPRKRTLLRSHPKLLAILGVLLLGIALWS
                     AMSGSKTAPKTVAPPPTTLSAHGTVVPIAQATVGTIGGGVVRSLAVKVGQTVNDHQLI
                     AEVSSPTQTETLVAPWGGTVIGLAVNRGDTLLPGAAVATIADLSGYQVQTTDVDEYLI
                     GQIHPNQQVAMTVEALDGRRLDGAVDTVALAQQTSGAGVVNYPIVI"
     gene            complement(711..1382)
                     /locus_tag="VHS06_00170"
     CDS             complement(711..1382)
                     /locus_tag="VHS06_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004183009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2986581.1"
                     /translation="MLDLTAVSRSYRVGDSDVWALRDATMHVADGDLITIVGPSGSGK
                     STLLQIIGLLDRPTSGTVSLDGQDLADLSDEGRARLRLVTIGFVFQRFHLLHDMTALK
                     NVALPMEAAGMRTEKRYERAALLLDAVGLSDRLLFMPAQLSGGQRQRVAVARALANNP
                     RLILADEPTGELHSEDKATIIALFRQLNADGRTVVMVTHDPEVASVARRRIEIRDGFV
                     SEATV"
     gene            complement(1385..2617)
                     /locus_tag="VHS06_00175"
     CDS             complement(1385..2617)
                     /locus_tag="VHS06_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2986582.1"
                     /translation="MAGEELEVTRSSSTGANRWPPLLAFPFPVRSAVRRWRGMLGMVV
                     GVGFALGLVMAMMGMIGTGMGQVLGDYAQSGANLYLVVNGGKLVVLKGADNPGTIDHA
                     SAVLSKVRGIPGVQAAVGELAWSLKQEREGPQARNQPTQFVPAMAVDGDPTEISNHVV
                     MREGRWLRRGNEVVIGPSLASSKSLRVGDSLRMNGRQFDVVGIGKLRGFGPSGDSVAY
                     LDARVLRGQAATGDVVNFIAIQTTAPQAVRNAVADLNSIRAISPDELTTEIKGSKDYS
                     SAIATYWLIDLFIFFVAGMFVSNMLGRSVAERRLEFGTLRAIGLPGKSILLSVAAEAL
                     CIILASFVFGVVVSLLLGWATNHWLAPAFNYDRLFDADPATYLAILVLSLGVGLIAGF
                     FPARAATRVDPLEVLREV"
     gene            complement(2607..3308)
                     /locus_tag="VHS06_00180"
     CDS             complement(2607..3308)
                     /locus_tag="VHS06_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2986583.1"
                     /translation="MARVVKQHAVRQREILDTAQRLVYSKGYEQMTVQDILDTLQIAK
                     GTFFHYFNSKQALLEALVDRMMEEMDQVIAPVMHDPNLSAIERLQSFFATTARWKVTQ
                     KVFLLQLLHVWYADHNAILRQKVRAAAFKRFAPFINAIACQGVQEGIFTTPYPEQIGE
                     VVFVLIEGLGDSFVEPLLANGPRLEDLPRAESAVCAYTDALERVLGAPHGSVQLIDGD
                     TLQEWFDLREEGDGR"
     gene            complement(3581..>4172)
                     /locus_tag="VHS06_00185"
     CDS             complement(3581..>4172)
                     /locus_tag="VHS06_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986584.1"
                     /translation="HAQATDLTVTQTPVGGSGVTATTTLTAVGNQTTITVAARGLTPG
                     STTNSEVHFGTVANPGAIAFDLGNLVAGADGTATATTTVNASLASLQDGNHFLHIHVL
                     NAEGAVLANGDIPMAAAVAAATPSVVTATPAATAAAVATATPSATVVAVAASPAALPS
                     AGEDPGAGSQAGLPGIVAAAAVVALLAGVVLRRRTI"
BASE COUNT          716 a         1310 c         1370 g          776 t
ORIGIN      
        1 gaattacgat tgggtagttc acgaccccag ccccggaggt ctgctgggcc agagccacgg
       61 tgtcgacggc cccgtccagc cgccgtccgt ccagcgcttc tacagtcatg gccacctgct
      121 ggtttggatg gatctgtccg atcaggtact cgtccacgtc ggtggtctgc acctggtagc
      181 cgctgagatc ggcgatggtt gccaccgctg cgccgggcag cagcgtgtcg cctcgattga
      241 ccgccaggcc gatgaccgtc ccgccccagg gcgccaccag ggtctccgtt tgcgtcggcg
      301 agctgacttc cgcgatcagt tggtggtcgt tgacggtttg gccgaccttc accgccaggg
      361 atcgcaccac gccgccacct atggtgccga cggtcgcttg agcgatgggg acgactgtgc
      421 cgtgggcgga tagtgtcgta ggaggtggtg ccaccgtctt gggtgcggtc ttgctcccgc
      481 tcatggccga ccagagcgcg atccccagga ggagcacacc gaggatggcg agcagcttcg
      541 gatggctgcg cagcagggtg cgcttccgcg gagccgcggc tccagttgtc gctttcggct
      601 cggtctctag cagactcatg cgggggaacc tccttggaca atccgtcgtg gattgcaggt
      661 tgcctgcgcc gaagcgtcca ggccgagacc tgccggagca cagggagttg tcatacggtt
      721 gcctccgaga cgaagccgtc tcggatctcg atgcggcgtc gggccaccga ggctacctcc
      781 ggatcgtggg tgaccatcac taccgtgcgc ccgtcggcgt tcagttgcct gaacagggcg
      841 atgatggtgg ctttgtcctc gctgtgtagc tcgccagtgg gttcgtcggc caggatcagc
      901 cttgggttgt tggcgagcgc tcgggcgact gctacccgct gacgctggcc gccggagagt
      961 tgagcgggca tgaagagcag cctgtcggag aggccgaccg cgtccagcag cagcgccgcc
     1021 cgttcgtagc gcttctccgt tctcattccg gcggcctcca tgggcaatgc cacgtttttg
     1081 agcgcagtca tgtcgtgtaa gagatggaag cgctggaaga cgaaaccgat ggtgaccagg
     1141 cgtaacctgg cacgcccttc gtcgctcagg tcggccaggt cctgcccgtc caggctgacg
     1201 gtgccgctgg ttggccggtc cagcagaccg atgatctgca ggagggtgct cttgccggag
     1261 cccgagggtc ccacgatagt gatgagatcc ccatccgcga cgtgcatcgt cgcgtcgcga
     1321 agggcccaga cgtcggaatc cccaacccga tagctgcggg agacggcagt cagatccagc
     1381 atcgtcacac ctccctcagc acttcgaggg gatcgacgcg cgtggcggcg cgggcgggga
     1441 agaagcccgc gatgagtccc accccgaggg agaggacgag tatcgcgaga tatgttgcgg
     1501 ggtcggcatc gaacagacga tcgtagttga aggccggcgc cagccagtgg ttcgtggccc
     1561 agcccagcag cagcgacacg accacgccga agacgaagga ggccaggatg atgcagagtg
     1621 cctccgcggc gacgctgagc aggatgctct tgccgggcag cccgatggca cgcagcgtcc
     1681 cgaactctag ccggcgctcc gcgaccgacc gtccgagcat gttgctgacg aacattccgg
     1741 cgacgaagaa gatgaacagg tcgatcagcc agtaggtggc gatggccgac gagtagtctt
     1801 tggaaccctt gatctcggtg gtcaactcgt cgggactgat agcgcggatc gagttgaggt
     1861 cggcgacggc gttgcgcact gcctggggcg cggtggtctg gattgcgatg aagttcacga
     1921 cgtcgccggt agcggcttgc cctcgtagca cccgtgcatc caggtaggcg acggagtctc
     1981 ccgacgggcc gaaccccctc agcttcccga tcccgacgac gtcgaactgc cggccgttca
     2041 tcctcagcga gtccccgacg cgcaacgact tgctggaggc caggctcggt ccgatcacca
     2101 cctcgttgcc acggcgcagc cagcgtcctt cgcgcatcac gacgtggtta gagatctcgg
     2161 tcggatcgcc atcgacggcc atcgccggaa cgaactgggt gggctgattt cgcgcctgag
     2221 gcccttcccg ttcctgcttg agcgaccacg ccagttcccc gacggcagcc tggacgccgg
     2281 ggattccgcg taccttggag aggaccgcgc tggcgtggtc gatggtgccg gggttatccg
     2341 cgcccttcag cacgacgagt ttgccgccgt tgacgacaag gtagaggttt gccccggact
     2401 gcgcatagtc gccgagcacc tgccccatgc ccgtgcctat catgcccatc attgccatga
     2461 ccagccctag cgcgaagccg acgcccacca ccatgcccag catccctcgc cagcgcctaa
     2521 cagcgctgcg gaccgggaag ggaaaggcca gcagcggcgg ccagcggttg gctcccgtcg
     2581 agctcgaacg tgtcacctcc aactcctcac cggccatcgc cttcctcccg caagtcgaac
     2641 cactcttgca gcgtgtcgcc gtcgatgagt tgcacggaac catggggggc acccagaact
     2701 cgctccaggg cgtccgtgta ggcgcagacg gcactctcgg ctcgcggcag atcctcgagg
     2761 cgggggccgt tggccaggag cggctcgacg aagctatctc cgagtccttc gatcaagacg
     2821 aagacaactt ctccgatctg ttcgggataa ggtgtggtga agatgccttc ctgcaccccc
     2881 tgacaggcga ttgcattgat gaaaggcgcg aagcgcttga atgcggccgc acgtaccttc
     2941 tggcgaagga tggcattatg gtccgcgtac cagacgtgta gcagttgcag aaggaatacc
     3001 ttttgggtga ctttccagcg ggccgtggtc gcgaagaagc tctggagtct ctcgatggcg
     3061 gacaggttgg gatcgtgcat gacgggggcg atgacctggt ccatctcctc catcatccga
     3121 tcgaccaacg cttccagcag cgcctgcttg gaattgaagt aatggaagaa tgtccccttg
     3181 gcaatctgga gggtgtcaag gatgtcctgg acggtcatct gctcgtatcc cttggagtag
     3241 acgagccgct gggcagtgtc gaggatctcc ctttgtctga cggcgtgttg cttgactacc
     3301 ctggccatcc catcatccca cctatagacc gaccgtcggt ctattatcga gccgcagtat
     3361 gggccggagc gccttccttg tcaacagttc aggaggtctg gctcatcgag gacaacacat
     3421 cggcggcatt ggcggacgat gcgagcggag cggaacaggg tgctggaact caccttgccc
     3481 gctccgatgt ttactcccct gtggataggg agatcgccgc ctgcccgtcg gcctgtgtac
     3541 cggctccgcc ctctgcttcc ctaccctctc tgaacgcgag ttagatcgtc cggcggcgca
     3601 agactacccc ggcgagaagg gccactactg ccgccgccgc cactatgcca ggcaggcctg
     3661 cttgggatcc cgccccagga tcctcacctg cactggggag agccgcggga gaggccgcta
     3721 cggctaccac cgttgccgac ggggtcgccg tagctactgc ggccgctgtt gccgcagggg
     3781 ttgctgtgac cacagacggg gtcgccgccg ccactgctgc agccattggg atatcgccgt
     3841 tggcgaggac ggcaccttct gcgttgagca catggatatg caggaaatga ttgccatcct
     3901 gcaaggaggc gagggacgcg ttgacagttg tcgtcgcggt ggctgtgccg tcggcgccag
     3961 ccaccagatt cccgaggtca aacgcaatcg cgccggggtt agcgacagtt ccgaagtgaa
     4021 cctcgctgtt ggtcgtgctc cccggggtaa gcccccgagc tgcgacagtt atcgtggttt
     4081 ggttacccac agcggtaagg gtggtagtag cggttacgcc tgaaccgccg acgggggttt
     4141 gggttaccgt caaatcagtg gcctgggcgt gg
//
LOCUS       DATALS010000009         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_1148,
            whole genome shotgun sequence.
ACCESSION   DATALS010000009 DATALS010000000
VERSION     DATALS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2065)
                     /locus_tag="VHS06_00190"
     CDS             complement(<1..>2065)
                     /locus_tag="VHS06_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX2986585.1"
                     /translation="LDEYKKKTGAKSDAELPADALKEIVKEYKEIVKAGTGKDFPNDP
                     FDQLRLAIGAVFGSWNGKRAIDYRNYNKIPHNLGTAVNVQTMVFGNMGWDSGTGVAFT
                     RNPSTGEKVLYGEFLVNAQGEDVVAGIRTPEPIARLQDQLPTVAEQFKGIAQRLEGFY
                     RDVQDMEFTVEHGRLFMLQTRAGKRTAQAAVKIAVDMVKEGIITKEEAIMRIEPNHVV
                     QLLLPNFDPKAKAAAAKEGRLLSKGLNASPGAAVGVAVFDADRAEEIGNTGQRVVLVR
                     QETSPDDVHGLIAAQGVLTSRGGATSHAAVVARGMGKPCVSGAEGIVVDVDKRQFTAG
                     GKVVKEGEVISIDGATGEVFYGEIPVSAASFAELTDLDQILKWSDETRKLGVWANADY
                     PRDAIKAREYGAEGIGLCRTEHMFMETDRLPIVQRMILAAPEAARLSAEIQKAKNELA
                     TTPSDKKAAVEAHISDLEKEAAGPVGNYRGALAELLPIQKGDFVGIFRAMAGLPVIIR
                     LIDPPLHEFLPDYETLLVEVTELRLKGGNPQELAEKERLLQAVESMREANPMLGLRGC
                     RLGILYPEIVEMQVQAIIGAACDLTKEGVVVKPEIMIPLVGHINELHLTKELLEPVAD
                     KVMKEAGVKVNYLFGTMIEIPRAALTADEIAKDAQFFSFGTNDLTQTTFGYSRDDAEG
                     KFLLQY"
BASE COUNT          348 a          674 c          621 g          422 t
ORIGIN      
        1 cgtactgcag caggaacttg ccctcggcat cgtctcgcga atagccgaag gtggtctgcg
       61 tgaggtcgtt ggtcccgaag ctgaagaact gagcgtcctt cgcgatctcg tccgcggtca
      121 gagcagccct cgggatctcg atcatcgtcc cgaacaggta gttgaccttc acgcctgcct
      181 ctttcatgac cttatcggcc accggctcca acaactcctt ggtgaggtgc agctcgttga
      241 tgtggccgac cagcgggatc atgatctcgg gcttgaccac cacaccctcc ttcgtgaggt
      301 cgcaggcagc gccgatgatg gcctggacct gcatctcgac gatttcgggg tagaggatgc
      361 cgaggcggca accgcgaaga cccagcatgg ggttcgcctc acgcatggac tccacagcct
      421 gcagcagccg ctccttctcg gccagctcct gcgggttgcc gcccttgagc cgcagctcgg
      481 taacctcgac cagcagcgtc tcgtaatcag gcaggaactc gtgcaggggc gggtcgatca
      541 gccggatgat aaccggcaag ccggccatcg cccggaagat gccaacgaag tcgcccttct
      601 gaatgggcag cagctctgcc agagcacccc tgtagttgcc gaccgggccg gcagcttcct
      661 tctccaggtc ggagatgtga gcctcgacag cggccttctt atcggaagga gtagtcgcga
      721 gctcgttctt cgccttctgg atctcggcgc tcaagcgtgc agcttcgggt gcggccagga
      781 tcatcctctg cacgatggga agcctgtcgg tctccatgaa catgtgctcg gttcggcaca
      841 ggccgatgcc ctcggcacca tactcgcggg ccttgatggc atctcgcggg tagtcggcgt
      901 tcgcccagac gcccaacttt ctggtctcgt cagaccactt gaggatctga tcaagatccg
      961 tcaattcggc aaaggaggcg gcgctgaccg ggatctcgcc gtagaacacc tcaccggtgg
     1021 ctccgtcgat ggagatgact tcgccttcct tcaccacctt gccgcccgcg gtgaactggc
     1081 gcttgtccac gtccacaacg atgccttcgg cgcccgatac gcagggcttg cccatcccac
     1141 gagcgaccac tgccgcgtgg ctggtcgcgc cgccgcggga ggtcaggacg ccctgggcgg
     1201 cgatcagtcc gtgaacgtcg tcgggggagg tttcctggcg aaccagaacc acacgctgac
     1261 ccgtgttgcc gatctcctct gctctgtcgg cgtcgaagac ggcaactccc acggcggcac
     1321 cgggggaggc gttgagcccc ttgctcaata gccggccctc ttttgcagcg gcagccttcg
     1381 ccttcggatc gaagttgggc agcagaagct gcacaacgtg gttcggctcg atccgcatga
     1441 tggcctcttc cttggtgatg atcccctctt tgaccatgtc aacggcgatc ttcaccgcgg
     1501 cctgggcggt ccgcttgccg gccctggtct gcagcatgaa cagcctgcca tgctctaccg
     1561 tgaactccat atcctggacg tcgcgataga agccctccag ccgctgggcg atccccttga
     1621 actgctcggc aaccgtgggc aactggtcct gtagtcgagc gatcggctcg ggggtccgga
     1681 taccggccac cacgtcctcg ccctgggcat tgaccaggaa ctcgccgtaa agaaccttct
     1741 caccggtgga cgggttgcgg gtgaaggcaa cgccggtgcc cgagtcccac cccatgttgc
     1801 cgaacaccat cgtctgcacg ttgacggcag tgcccaggtt gtgggggatc ttgttgtagt
     1861 ttcggtagtc gatggcgcgc ttgccgttcc aggatccgaa cacggcgccg atggccaacc
     1921 gaagctgatc gaagggatcg ttggggaaat ccttgccggt accggccttt acgatctcct
     1981 tgtactcctt gacgatctcc ttcaacgcgt cggcgggcag ttcggcatca ctcttggcac
     2041 cggtcttctt cttgtactcg tccag
//
LOCUS       DATALS010000010         3502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_361556, whole genome shotgun sequence.
ACCESSION   DATALS010000010 DATALS010000000
VERSION     DATALS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361556"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..846)
                     /locus_tag="VHS06_00195"
     CDS             complement(64..846)
                     /locus_tag="VHS06_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2986586.1"
                     /translation="MGELIHCTVDRGIATLVLDNPPVNVVTLALTDDLERRLRDLAED
                     PAVRVLVVTGAGSKAFCAGSDIQEFVQYMAPGVVIDKKMAKEIRVYDALDSFPKPTIA
                     AINGFAMGGGAELALCCDIRVMDEGKKIGFPECKLGVFPGSGGTQRLPRIVGEGIAKE
                     LMYTGDPIGAQRAYEIGLINRIAPAGEALALAMELANTISQRAGIALECIKETVDYGL
                     AAGICEGQQRVLKLTDRVFRSEDIQEGVKAFMEKRPPQFKHR"
     gene            complement(869..1663)
                     /locus_tag="VHS06_00200"
     CDS             complement(869..1663)
                     /locus_tag="VHS06_00200"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2986587.1"
                     /translation="MSVQSAVLRQRRLELAMGEWENATVLVTGGNSGMGLAAAKLFAA
                     AGAKVAAGDLRTESVQAVGSDLLFPCILDVSDERSVQECVEWVERELGPLSVLVNCAG
                     TTVVADAPHTSVADLDRVLGVNLKGVFLTCKHVIPYMINRKGGAIVNVASHAGLVGMR
                     ERAAYCASKGGVVSLTKALALDHVLQGIRVNAVCPGTTDTPWMERVAQLAPDPDEARR
                     QFAARQPMGRMGRPEEIAAAVEFLASSRASFITGTTLLVDGGWTAE"
     gene            complement(1774..2928)
                     /locus_tag="VHS06_00205"
     CDS             complement(1774..2928)
                     /locus_tag="VHS06_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2986588.1"
                     /translation="MDFQPTETQKMIRDMARQFATEYVEPRAKEIDEKDEFFPDLIEK
                     AGELGLLGMELPEEDGGAACGTVAAVMSGEEIGRVSAAVCNVMGAIRLHLALLSKFGT
                     AAQKEKWLEALSSGRKIGGFAITEPGAGSDVASIKTRAVKRGDKWVINGQKIFITLAP
                     VSDMMIVLTSTDPEKGSRGETCFIVEKGTPGLTIGPKDPMMGQHGVPVSQVYFDDCEV
                     PAENLFGEEGKGFKVMMAGLDGTRLDIAALSLGLSQAAFEQAASYAATREQFGQPIGE
                     NQGIGFMLAEMATEIDAGRMLLYRAAWMRDAGLPFTKESSMAKFFCADNAMRHTTNAV
                     QIFGGYGYTKEYPVERYMRDTKVCLIYDGTSQIHRMIIARKILKEFADRA"
     gene            complement(3321..>3502)
                     /locus_tag="VHS06_00210"
     CDS             complement(3321..>3502)
                     /locus_tag="VHS06_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2986589.1"
                     /translation="TAGVHDKYSKLIGEGLTVQNRWGTPEDIGKVVAAMVRGDLAYST
                     GQVVRVDGGLQIGRL"
BASE COUNT          602 a         1283 c          884 g          733 t
ORIGIN      
        1 ggaacgttct cgtcgcgatg agaacgttcc gctgaaccct ccactccgtt tagtggacgt
       61 gccctacctg tgcttgaact gaggggggcg cttctccatg aaagctttga cgccttcctg
      121 gatgtcttcg cttcggaaca cccgatctgt aagcttcagc accctctgct gcccctcaca
      181 gatcccggcc gccaggccat agtcgaccgt ctccttgatg cactccagcg cgatcccagc
      241 tcgctggcta attgtattgg ccagctccat ggctagggcc agcgcctctc cggccggcgc
      301 aatccggttg atcaacccga tctcgtaggc cctttgggcg ccgatcggat cgccggtata
      361 catgagctcc ttcgctatac cctccccgac gatccggggc agccgctggg tcccgccgct
      421 gccggggaac acgcccagct tgcattcggg gaagcctatc ttcttgcctt cgtccatcac
      481 ccggatgtcg cagcagaggg ctagctcggc gcctccaccc atggcaaagc cgttgatcgc
      541 ggctatcgtc ggcttgggga agctgtccag ggcgtcgtat accctgatct ccttcgccat
      601 cttcttgtcg atgacgactc cgggtgccat atactgcacg aactcctgga tgtcggagcc
      661 agcgcagaac gccttgctcc cggcgccggt caccaccagc acccgcaccg ccggatcttc
      721 cgcgaggtct cgcaggcgtc gctccagatc gtcggtcaaa gccagcgtca ccacgttcac
      781 cggtggatta tccagaacca aggtcgcgat gccgcggtcc accgtgcaat gaatcagttc
      841 gcccatgact aaaccgctcc taatgttact actcagcagt ccatccgccg tcgaccagca
      901 acgtcgtgcc ggtgatgaaa gacgctcggc tcgaggccag gaactcaacg gcggcagcga
      961 tttcctcggg gcggcccatc cgccccatcg gctggcgcgc ggcaaactgc cgacgcgcct
     1021 cgtcggggtc gggcgcaagc tgcgcgaccc gctccatcca gggggtgtcg gtagtgccgg
     1081 gacaaacggc gttgaccctg atcccctgaa gcacatgatc cagcgccagt gccttggtga
     1141 gggacactac tccccccttc gaggcacagt aggccgccct ctccctcatt ccgaccaggc
     1201 cggcatggga cgccacgttg acgatagcgc cgcccttccg attgatcata tacgggataa
     1261 cgtgcttgca ggtcaggaac actcccttga gattgactcc cagcaccctg tccaggtctg
     1321 ccacgctggt atgcggcgcg tcggcgacta cagttgttcc cgcacagttc accagaacac
     1381 tcagcggtcc taactcccgc tcgacccact ccacgcactc ctgcacagag cgctcgtcgc
     1441 tcacgtcgag gatgcaggga aagagcaggt cggagccgac cgcctgaacg gactccgtgc
     1501 gcaggtcccc tgcggctact ttggccccgg ccgcggcgaa cagcttcgcc gccgccaagc
     1561 ccatgccaga attgcctccc gtcaccagga cggtagcatt ttcccattca cccattgcca
     1621 actccaggcg ccgttggcgg agcactgcgg attgaacgct caccagcact ccgcctcgcg
     1681 acgcatcgta acgatgtcgt tctcctaccc acgattgaac tgcgcgatgc tcagcaatga
     1741 atcacttcat tcatcgccca actgctcctg tggctatgcg cggtcggcga actccttgag
     1801 gatcttcctg gcgatgatca tccgatggat ctgcgaggtg ccgtcgtaga tcaggcacac
     1861 cttcgtgtcc ctcatgtacc tttccaccgg atactccttg gtgtatccgt atccgccgaa
     1921 gatctgcact gcgttcgtcg tgtgcctcat cgcattgtcc gcgcagaaga acttcgccat
     1981 cgacgactcc ttcgtgaacg gcaatcccgc gtccctcatc cacgccgcac gatatagcag
     2041 catccgccct gcgtctatct ccgtcgccat ctccgccagc atgaacccta ttccctggtt
     2101 ctcccctatc ggctgcccaa actgctcccg ggtggccgca tagctcgctg cctgctcaaa
     2161 cgccgcctgc gataagccca gcgacagcgc cgctatgtcc agccgagtcc cgtccagccc
     2221 ggccatcatc accttgaacc ccttaccttc ctctccaaac aggttctccg ccggaacctc
     2281 gcagtcgtca aagtacacct ggctcaccgg caccccgtgc tgccccatca tcggatcctt
     2341 cggccctatc gtcagcccag gtgtcccctt ctccacgatg aagcaggtct ccccgcggct
     2401 ccccttctca ggatcggtcg acgtgagcac tatcatcatg tccgataccg gcgccagcgt
     2461 tatgaagatc ttctgcccgt tgatgaccca cttgtcccct cgcttcaccg ccctcgtctt
     2521 gatgctcgct acgtccgacc ccgcaccagg ctccgttatg gcaaagccgc caatcttccg
     2581 cccgctgctc aacgcctcca gccacttctc cttctgcgcg gccgtcccga acttgctcag
     2641 tagcgcaagg tgcaacctga tcgctcccat cacgttgcac accgcggcac tcacccgtcc
     2701 gatctcctcg ccgctcatca ccgcggccac cgtcccacat gctgcgccgc cgtcctcctc
     2761 aggcagttcc atccccagca gccccagctc gccggccttc tcgattagat cggggaagaa
     2821 ctcgtccttc tcatctatct ccttcgctct cggctccaca tactcggtgg caaactgccg
     2881 cgccatgtcc cggatcatct tctgggtttc ggttggctgg aaatccatct cctctggatc
     2941 ctcctcctgg aaatcttctg ggtaggaaag gcagcggccg cgctgccctc tgattaagat
     3001 gcctgcaggg cctcttcagt taggcaaccg gttgccatta tacacacttc agcccccact
     3061 gtccgtcctt ttcccccggc ttgtcggcaa ggtggttctt gggaacggca acgcccctcc
     3121 tgcttcactt tcgcaggagg ggcgttgtcc ccaccaagcc tgtatgcagc gtccgcctag
     3181 tcctctgaca cagtagtccc gatttctagg aacccagcgt aacgcgacgc tcgactctta
     3241 gtgttgagat cggcgtgtca gagtactggg ccccgctctg ccggatagct acccgggcac
     3301 ggtctcgtca aaccgcggct ctacagcctc ccgatctgca gccctccgtc tactctgacc
     3361 acctgccccg ttgagtaggc cagatcgcct cggaccatcg cggcgaccac cttgccaatg
     3421 tcttcggggg taccccacct attctggacc gtgagcccct cgccgataag cttgctgtac
     3481 ttgtcatgta cccccgcggt ca
//
LOCUS       DATALS010000011         6191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_1244,
            whole genome shotgun sequence.
ACCESSION   DATALS010000011 DATALS010000000
VERSION     DATALS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1097
                     /locus_tag="VHS06_00215"
     CDS             342..1097
                     /locus_tag="VHS06_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2986590.1"
                     /translation="MFQKDTFKGKVAVVTGAAAGIGEAVARGLAAHGADVVLLDVRAE
                     DLERIAVELRELGTRSFAAPADVTSSAQVQTVVGQCIQNLGRVDILVNAAGGFKSRCP
                     VAEMPDDMWNSIVNLNLTGVFVATRAALPRMIEQRYGRIVSVSSDAALASRHISNAAY
                     AAAKAGIVAFSRHLALEVGQYNITVNATAPGVTLSSRIRNLYNDKQLAEFAAKNPLGR
                     VGELEDQVLPILFLASDAAKHITGEVLKVSGGY"
     gene            complement(1440..1754)
                     /locus_tag="VHS06_00220"
     CDS             complement(1440..1754)
                     /locus_tag="VHS06_00220"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEX2986591.1"
                     /translation="MRTATNAVPSATLICFIGLNLADIASTFIGLRMGLRESNALPSI
                     LYGLAGEFGIYWLKMLVLCLVALLVVRLSVYWPKVWRSLVVANVIIAAVVIGNIMMLV
                     TG"
     gene            2090..2515
                     /locus_tag="VHS06_00225"
     CDS             2090..2515
                     /locus_tag="VHS06_00225"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX2986592.1"
                     /translation="MRTAIAGLLALVTVGVATFSVVTPGAAQAVDLGEGSAETTLDTS
                     APEAVQSQEPVDLGTVQTTAYSLRGAMKSGEYTYSGAVAVDPEVIPLGSTIYIEDLGY
                     FVAEDTGSAVKGSHVDIWFPSYREAIQYGVQYRRAWLIG"
     gene            complement(2642..3646)
                     /locus_tag="VHS06_00230"
     CDS             complement(2642..3646)
                     /locus_tag="VHS06_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2986593.1"
                     /translation="MSSGNEPEVYDITIIGAGPVGLYGLYYTSMRGMRAKVVDSLPQV
                     GGQLMALYPEKYIYDVPGFRKVLAKDLIHNLEDQAFQHNPTVCVNERIATLTRGEDGI
                     ITLVGESGNVHLSRTVLIAAGVGAFVPRKLDVPKLDVLEGRGVHYFVKNMADFKDKRV
                     LIVGGGDSAVDWAMALEPIAKQIMLIHKFEQFQAHEKSVEDLRGTSTIIKVPYELKTI
                     HGEEHVEGVTIFQNKTGVEEEHDVDALFMNIGFVTNLGPIKSWGLEISKNSVLVTAQM
                     ATNIPGVYAAGDICTHPGHLKLISVGAGEAAVAVNNAKSFIDPTARVEPGHSSHKKQE
                     "
     gene            3872..5194
                     /locus_tag="VHS06_00235"
     CDS             3872..5194
                     /locus_tag="VHS06_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX2986594.1"
                     /translation="MTRLVPLLVAVPVFAGVVLASGVLGLGSPAGTADQAVPVAAQAA
                     QPSSTPVPAAAPTFVPAPAATPKPAASTQRASDGGKKVVVGYYVPYDTTSWTSLQNQA
                     DSLDYVDTQWVNVDACGNVGSRDDNTLIAYARDKGVKVLPSLLTGSDWLNHRLLTNPA
                     IADRYLSQIVSYVVEMGYPGFDLDMENIGDADRDAYSTFVAKLADALHQRGKILTLAI
                     PAKTTDVRTGWAGPYDYAALGKSADLILLMTDDYSWSTGPAGSIAPKDWVDKVAAYAV
                     SQMPLEKVLVGLAFYGYDWNKTQAGTKARGLQYAQSVAIAKQHGAQITTDPTTGSATF
                     SYTARAGEMVPNTSPPADSKHDISVRQLGQCAVQEPAAPSPKPTPTPVPVQERTVWLE
                     DAAAVASKLDVAVKRNVGGVGSWRLGQEDPAVWPLLARYRSGTATPGK"
     gene            5812..>6191
                     /locus_tag="VHS06_00240"
     CDS             5812..>6191
                     /locus_tag="VHS06_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986595.1"
                     /translation="MASTTPTGTMLPTETPTPTQTDTPTTTPEPTFTPWPSWTPWPTA
                     TQGVAPSNDDIDSALLVSYLPYTASIDTTGATVASDDPNMGSNTGVNSNTVWFRYVAL
                     APGSVQVSTVGSTYDTVLAVYTGS"
BASE COUNT         1243 a         1873 c         1825 g         1250 t
ORIGIN      
        1 ccatcgccgt agtcgggctc caggaggagc aggctgccgc agtccgggca ccgtctcttg
       61 aggccggacg gcgtgtttct tagggtgggg atctccgcaa tcaactccag gatactcctt
      121 cgcttcttaa accatgtttc caagcggcga aggatagcgg gtctcgcgga ttcatgcaag
      181 ccctaggtta acagagagtt attgtcccga tgcgaaccga tttggagtgt gacataatgg
      241 cccgcaaccg cgccgaggtc tatctccccg aagttctcac ttagcggtgg catgcctcca
      301 gtcccaaatc ggcgcggacc acaagtattg gaaggacgaa gatgttccag aaggatactt
      361 tcaagggaaa agtagcggtt gtaacaggcg ccgccgcggg cataggagag gcggtagcga
      421 ggggactcgc cgcacacggc gccgacgtgg tgctgctgga tgtgagggcg gaagacctgg
      481 agaggatagc cgtcgagctc cgtgaacttg gtaccaggtc atttgccgct ccggcagacg
      541 tcacctcttc agcgcaagtg cagacagttg tgggccagtg tatccagaat ctgggtagag
      601 tggacatcct ggtgaacgcc gccggcggct tcaagtcgcg gtgcccagtg gctgagatgc
      661 cggacgacat gtggaactcc attgtgaacc tgaatctgac cggcgtgttc gtggccacca
      721 gagcggcgct gccaaggatg atcgagcagc gctacggtcg catcgtctcc gtctcctccg
      781 acgccgcact ggcttcaagg cacatctcca atgcggctta cgccgctgcc aaagccggta
      841 ttgtggcgtt ctccaggcat cttgccctgg aggttgggca gtacaacatc acggtgaatg
      901 cgactgctcc aggcgtcacg ttgagcagcc gaatccggaa cttgtacaac gataagcagc
      961 tcgcggagtt cgccgcgaag aacccgctag ggagggtcgg ggaactagag gaccaggtgc
     1021 tccctatcct cttcctggcc tcagacgctg caaagcacat cactggcgag gtgctgaagg
     1081 tgtccggggg gtactagcct ggcctcaccg cggagcccgc gcagcaccac ctcgccacct
     1141 ggcagggcac agggccctgc actaccggaa ctcgagcggg agcggactaa gccttccaca
     1201 cttcgaacag cgggcgtagt gaaccctgtt gtcgatccgg acctccgtca cttcgaccgc
     1261 gagagcggag gtcccgacga ctgctggcat atgccagggc ccgcacgtcc cttcctcgat
     1321 ccgtatctgc gtccgtgtac ggcagtttac gcagtactcc atccagcggt gcttcttgcc
     1381 ctgagagtca atcttgtaag ctagtccatc gatctctatc cattcgttca tcgcgttatc
     1441 tagcctgtca ccaacatcat gatgttcccg attaccacgg ccgcgatgat gacgttggcc
     1501 actaccaggg atcgccagac ttttggccag tagactgata gcctgacgac gagaagagct
     1561 accaggcaca gcaccagcat cttcagccag taaatgccga actcccctgc caagccgtac
     1621 aagatggagg gaagggcatt gctttcccgc aggcccatcc ggaggcctat gaaggtagaa
     1681 gcgatgtccg ctaagttcag ccctatgaaa caaatcagtg tcgccgaagg cactgcattt
     1741 gttgctgtcc gcatcaccat ttcctcccag tgatcatcta gcttgacggt aggccagtag
     1801 ggcgtcacac gatcattttg gaggaggctc attacttgga catgtctaga gccgaccgga
     1861 agccgcatta tctgtaatct cgggagggcg atgtacttga ggagacccga agaaggggga
     1921 gctcaggcct tcagtggttg ccgcgatacg agtggggttg taagtagttt ggcttggagt
     1981 tattagttat atgggttagt attgtcgcgg gttcccaatc tccggaaagg aggcatcttt
     2041 acaaggtcgc agggcgcgac cacaatctcc caaccttagg aagaagagga tgcgaacagc
     2101 aatagcgggt ctgctggccc tggtcaccgt cggggtggcc acgttttcag ttgttacacc
     2161 aggagcagct caggcagtcg atctgggcga gggcagcgca gaaacaacac tcgataccag
     2221 tgccccggaa gcggtgcagt ctcaggagcc ggtagatctc ggcaccgtgc agacaacggc
     2281 atattcgctc aggggtgcga tgaagagcgg ggagtacacc tattcgggag cagtagcggt
     2341 agaccccgaa gtaattcccc ttgggagcac gatctatatc gaggacctgg gctactttgt
     2401 ggcggaggat accggtagcg cggtgaaagg gagccatgta gacatatggt tcccttctta
     2461 ccgggaggcg attcagtacg gagtccaata tagacgcgcc tggctgatcg ggtagaagcg
     2521 caccgtcgcc cgtcatcccg aagcgcagcg aaggatctcc tcgtcccatc gcggagattc
     2581 ctcgcttcgc tcggaatgac agttctagtt gcttcgttcg gaatgacggg tttaggcagt
     2641 cctattcctg tttcttgtgg ctcgagtggc cgggctccac acgggccgtg gggtcgatga
     2701 agctcttggc gttgttcacc gcgacagccg cttcgcccgc accgaccgat atcagcttga
     2761 ggtggcctgg atgagtgcaa atgtcgcccg cggcgtatac gcccgggata ttggttgcca
     2821 tctgggcggt aactagcacg ctgttcttcg atatctccag tccccagctc ttgatggggc
     2881 cgaggttggt aacgaagccg atgttcataa acagcgcgtc gacgtcgtgc tcctcctcta
     2941 ctccggtctt gttctggaag atagttaccc cttcgacgtg ctcctcaccg tgtatcgtct
     3001 tcaactcgta cgggaccttg atgatggtgg acgtgccgcg taggtcctcg acgctcttct
     3061 cgtgtgcctg gaactgctcg aacttgtgga tcagcatgat ctgtttggct attggctcga
     3121 gagccattgc ccagtccacc gcggagtccc cgcctcccac gatcagcaca cgcttgtcct
     3181 tgaaatcggc catgttcttc acgaagtagt ggacgccgcg gccctccagc acgtcgagct
     3241 tcggcacatc cagcttcctc ggaacaaaag ctcccactcc ggcggctatg aggacggtcc
     3301 ggctcaggtg aacgttgccg ctctcgccga cgagggtgat gatgccatct tctcctctag
     3361 tcagggtcgc tatcctctcg ttcacgcaca ctgtcgggtt gtgctggaat gcctggtcct
     3421 ccaggttgtg gatcagatct ttggccagca ccttacgaaa gccagggaca tcgtagatgt
     3481 acttctccgg gtagagcgcc atcaactggc cgccgacctg gggaagagag tccacgacct
     3541 tcgctctcat ccctcgcatc gacgtgtagt agagaccata gagaccgacc gggcccgccc
     3601 cgatgatcgt gatatcgtag acctcaggct cgtttcccga cgacattctc cacctcgctt
     3661 cgggttctcg cgccggcgca gtttgcggcg tcgtgtcgcc atcatagcac ttccgactgg
     3721 cgagttcttg cctcagaccc ttgtgataac gcgcacgata tggcagactt ctgttgtcgg
     3781 tgtaacccgt taaggtgttc cctgtgcctc catcttcttc ccgtgcctcc ttgtggcagg
     3841 cgcatgggcg ctcctcgcga aaggtgcttg catgacccgt ttagtgcctc tcctcgtagc
     3901 cgttcctgtg ttcgccggag tagtactcgc cagcggtgtg cttgggttgg gaagtcccgc
     3961 cggtaccgcc gaccaggctg taccagtagc cgctcaggca gcccagcctt cttcgactcc
     4021 ggtccccgcc gccgcgccta ccttcgtgcc ggcgcctgcc gcaactccta agcctgctgc
     4081 ttctactcaa cgggccagcg acggcggcaa gaaggtggtc gtggggtact acgtcccgta
     4141 cgatacgacg tcctggacat cccttcagaa ccaggcggac tcgctcgact acgttgacac
     4201 ccagtgggtg aacgtggatg catgcgggaa cgtgggcagc agagacgaca acaccctcat
     4261 cgcctatgcc agggacaagg gagtgaaggt gctgccctcg ctgctcaccg ggtccgactg
     4321 gctgaaccat cgcttgctca cgaaccccgc gatagcagat cgttaccttt cgcagatcgt
     4381 cagctacgtc gtggagatgg gctatccggg cttcgacctc gatatggaga atatcgggga
     4441 tgcggacaga gatgcctact cgacgttcgt cgcgaagcta gccgatgccc tgcaccagag
     4501 gggcaagatc ctgacgctgg caatccctgc caagaccacg gatgtgcgga ccggttgggc
     4561 gggcccctac gattacgcgg cgctcgggaa gtccgcggac ctcatcctct tgatgaccga
     4621 cgactatagc tggtcgactg gccccgcggg ctcgattgcg ccaaaggact gggttgacaa
     4681 ggtcgccgcg tacgccgtga gccagatgcc tctggagaaa gtgctggtcg ggctggcgtt
     4741 ctatgggtac gactggaaca agacccaggc cggcactaag gcgcgggggc tgcagtacgc
     4801 gcagtccgta gccatcgcga agcagcacgg ggctcaaatc acgaccgacc ccactacggg
     4861 ctccgcgacc ttcagctaca cagcaagggc tggcgagatg gtgccgaaca cgtccccacc
     4921 tgcggactct aaacatgaca taagtgtcag acagcttggt cagtgcgccg ttcaggagcc
     4981 cgctgcaccc tccccaaagc cgactcccac acctgtgccc gttcaggagc gaaccgtatg
     5041 gttggaagac gccgccgcgg tagccagcaa gcttgacgtc gccgtcaagc gaaatgtcgg
     5101 gggtgtcggt tcgtggcgcc tagggcaaga ggacccggca gtgtggccct tgctagcgag
     5161 gtatcgctca ggaaccgcga cgcctgggaa gtagacccct cgttctttcg acacatgtaa
     5221 taatggacat cttgccggcg ctctccgatt cgagagcgcc ggcattcttt ctcggaaccg
     5281 tcgaacaata tggaaaataa tctgttaggt gctaatccaa caagagcgtc atgacgattg
     5341 gcggtccggc gcatacaatc gttgatatct gggcacgcaa tattaagtgg gaatacactg
     5401 ggctgactgc cctgtgtgct ttttcgggca agagggggca gggccgtgag aaggaactct
     5461 cgtcttccac tgctggtggt agcggtattg acaactgcaa tggctgtgtt gtcgctgctc
     5521 cacatgccca tctcgggggc ggtactaagt actgcggctg ccgctgaaaa tagcgacgtg
     5581 atcgcagctc cgaaaccgtc gccgaccctg ccaccaaagc ccgtcgcttc agtagccaca
     5641 gcgacgccaa cccccacttc tgggaccacg agcacgagcg ttccgacagc gacgagcacg
     5701 ccgagccaaa cggcaatccc aactgccgcg aacacgagta cgtccacggc cactcccgcg
     5761 tccaccagca ccccgacgag ttccagcact ccaacggcca caagcaccct gatggctagc
     5821 accaccccta ccggaactat gctgccaacc gagactccga cgccaactca gaccgatacg
     5881 ccgactacca caccggagcc cacgttcacc ccctggccgt catggacccc gtggccgacc
     5941 gcaacgcagg gcgtggcgcc gtccaacgac gatatcgact ctgctttgct ggtctcctac
     6001 ctaccgtaca cggcgagcat tgacactacc ggcgcgacgg tggcttcgga cgatccgaac
     6061 atggggagca acaccggggt caactccaac actgtctggt tccggtacgt ggcgctcgca
     6121 ccgggcagcg tgcaggtgag cactgtcggg agcacctacg ataccgtact cgcggtctac
     6181 actgggtcca g
//
LOCUS       DATALS010000012        10081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_316047, whole genome shotgun sequence.
ACCESSION   DATALS010000012 DATALS010000000
VERSION     DATALS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10081
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316047"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..634
                     /locus_tag="VHS06_00245"
     CDS             47..634
                     /locus_tag="VHS06_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX2986596.1"
                     /translation="MKRLLLSLSLVVLVAVVFGVAVNAQSDAQPKSGRTGSEKVPAPV
                     VKASVTRIVDGESIEVDIPGRGGVKVRYLGISTPESSDPRRSDEYLVEVATTRNRGLV
                     DGKTVYLEKDATDADKEGRLLRYVYLADGTFVNQQLAKDGFAVAKVVPPDVKHRAALE
                     AAEKDARNHSRGMWGPVEAFGPMEILPVVNPTPVP"
     gene            complement(661..1035)
                     /locus_tag="VHS06_00250"
     CDS             complement(661..1035)
                     /locus_tag="VHS06_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986597.1"
                     /translation="MALFCFSAQILPGKTDQWKRMTEEMLGSRRQQYEECRGKAGITR
                     ELAWLQHTATGDVVSVFWEVADPQGAFEHLSRSQDPFDQWNRERISEILGWDWQRISK
                     EPIPELAFDWESIQAQVQEEAA"
     gene            1198..1956
                     /locus_tag="VHS06_00255"
     CDS             1198..1956
                     /locus_tag="VHS06_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2986598.1"
                     /translation="MRRRTGRFLTTAQVAAAVGVHPNTVRLYEAWGFLPPIPRGPNGY
                     RRFTEAHLDLMRLARTALQYPYPGGKEPVLGLLERAKAGDLEGALELGRGYLDQVRGE
                     RAKAEEAVVILEGWARRGMVSRAGRLLRIGEAASLLETTVDALRHWERNGLIEVPQDP
                     RNRYRGYGPLELDRLRVIRVLRSAGYSTMSILRMLRHLDEGSTDDLRHVLDTPHPDDD
                     VIYWTDRWLSSLAEQERRAMDVIALLHEMLEKRG"
     gene            2084..3322
                     /locus_tag="VHS06_00260"
     CDS             2084..3322
                     /locus_tag="VHS06_00260"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX2986599.1"
                     /translation="METIHETETMSHLRRLCEIGPRPIGSKANHAAAEYIRGVFRSAG
                     LEVVDQTFQCPDWETRESWAEIEGLRLRAEANVFSPSVDVTAPTVALGSIPELERSEL
                     QGRIGVLYGDLAARPLTPKGCTIYQHERDQRVNRLLEEKRPLALITVSPRIGSVERVI
                     EDWSLEIASATVPPEVGLSLLQRIGDPVRLRIVTESSLGYSCNVVADRPGVREGRIVI
                     CAHYDTKIETPGAGDDAAGIAAVLTLAHLLPARYPGVGLEFIAFSAEEYGASQDDTEF
                     YVRCRGDAMGGITAAINMDLVGQRLGTTAISMMASSETFRDSVDALLKERAGLIWTEP
                     WPESNHSTFALRGVPAVAINCKGITNLTHMPEDTMDWIGEKKLGEVVEIVSAIVGGLQ
                     DKSVDWTREARCDLDRTTAI"
     gene            3339..4004
                     /locus_tag="VHS06_00265"
     CDS             3339..4004
                     /locus_tag="VHS06_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX2986600.1"
                     /translation="MLPRVVLHNAVSIDGRIDGFTPDLGLYYGLIPRWAEDATLVGTE
                     TVLAADLGAPEEEETEDPPPVSAGDRRPLLVIPDSRGRVRAWDRLRKQPYWRDAVSLC
                     SRSTPLEHLAYLSERRVDHIVAGEDKVDLRAALEELNIRYGVKVVRADTGGMLNGALL
                     QAGLVDEVSLLIAPCLVGSSTPKSIFQLPDELKKGIEIPARLMHLERMAGDWVWLRYE
                     LAK"
     gene            complement(4032..5051)
                     /locus_tag="VHS06_00270"
     CDS             complement(4032..5051)
                     /locus_tag="VHS06_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifH"
                     /protein_id="HEX2986601.1"
                     /translation="METGWVSTLRGNPLPWLLEEGNPAVRQMALRFLLDRPEDDPEVI
                     RARGDALKSNPIAAILAAQDPRGYWVKPGPGYGPKYMGTVWQVIFLDQMGADSSDARI
                     RAACDYVLTVCPTSSGGFGCSASSEEKAPPPSAVIHCLHGNLLRALIGFGRQEDPRVQ
                     RAIDWQARAITGEGFDRFYKSSTSGPGFCCAANNGKPCAWGAVKAMLALARVPEERRS
                     TAVRRAIEQGSDFLLSCDPATAAYPTAHGEKPSRSWFKLGFPLGYVTDLLQILEALCE
                     HGMARDPRLQRAVQWLLEQQDSDGRWRNRYTYNGKTWVDLERLGEPSKWVTLRACRVI
                     KAVLA"
     gene            complement(5227..6231)
                     /gene="dusB"
                     /locus_tag="VHS06_00275"
     CDS             complement(5227..6231)
                     /gene="dusB"
                     /locus_tag="VHS06_00275"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX2986602.1"
                     /translation="MQVSDSRTLRIGSLHLQTPILLAPMAGYTDLPFRSIVRPLGGLG
                     LAFAEMMNPPSILFGKGKRRTELLATTPEDTPLGHQIYGTDADLMARAAQWLEEQGTD
                     LVDINMGCPQREITAGNAGAALLKQPKEAVRIAEQVVRAVSVPVTAKIRLGWDAESIV
                     AADLARQLEGVGIAAITVHGRTRQQGYGGRADLDEIRKVVEAVEGIPVIGNGDITSPL
                     AARRMLEETGCAGVMVARGATKDPWLIRNIWRELSRLSALPAPSQAEKVAILREQFER
                     SILHYGQLHAVAIFRRWIPPRASSLGLSREKMLQILKIRELPEMRAALEQLQNEAAAL
                     "
     gene            6452..7090
                     /locus_tag="VHS06_00280"
     CDS             6452..7090
                     /locus_tag="VHS06_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX2986603.1"
                     /translation="MSNQFELVVEERSVLGKQVKRLRRDGIVPANLYGHREPSLPLQV
                     PSAVLSRLFSQGGATTLVQLKIGAKPPVQALVKEVQHDPTTGSVKHVDFFRVAATEKF
                     RTHVPLHFINEAEAMKLGDVAVQRSLADVLVECLPADLPAAITVDLAKLGEIGAVIRV
                     GELDVPEGVSVLLDHEEVVAGVHRVAREAEPEVEAAAPAEGAPAPTEEGEGA"
     gene            7478..8185
                     /locus_tag="VHS06_00285"
     CDS             7478..8185
                     /locus_tag="VHS06_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX2986604.1"
                     /translation="MTHVVDDYDILRTEGLSRSVAGKTLVDGITVGMKQGDVLAVVGP
                     SGAGKSSFLRLLNRLDEPTEGTVYLDGRDYRTISPRELRRRVGMVMQSPFLFPGTVGD
                     NLVFGPRQWGESPTEETVEGWLARVGLAGYVGRNVATLSGGEAQRVSLARTLANSPEV
                     LLLDEPTSALDEAAKAGIEALIRDILADGRLTCVMVTHDMAQAERMANRVMVLEGGRV
                     ARVGPVEEVIRAHSSLH"
     gene            8163..8978
                     /gene="fetB"
                     /locus_tag="VHS06_00290"
     CDS             8163..8978
                     /gene="fetB"
                     /locus_tag="VHS06_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEX2986605.1"
                     /translation="MLTQVFTNPVSLGLAQAAVVSFLSIAVVLMASRRGIRVERETLV
                     ALARGLVQIVAVGSVLVVLLNGPIWTSIIVLCAMAVAAASTSARRAKKVRGAFWDSLW
                     GVGLGAGSVIAVMTLVGVIEPGVDSVVPVGSMIIANAMNSVAIAMDRFRSDVEAHTGQ
                     IEAGLALGADPKTVVAPYVQSSVQASMIPRIDNLRSLGIVWIPGLMAGMILSGSDPVY
                     ASIYQFVVLGMIFASAGLTSVISLMLFRRRAFSTAEQLVMRPSASVGGGSSSR"
     gene            9009..>10081
                     /locus_tag="VHS06_00295"
     CDS             9009..>10081
                     /locus_tag="VHS06_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2986606.1"
                     /translation="MRSYRVPMVPGPTRVPADVSAAYQAEYGSSDLEEEFFDLYGRVQ
                     EQLQQLLMTRSQMAIMTGEGMAALWGALKSTIRPGDRVLSVATGLFGYGIGEMARAVG
                     AHVEVVEFPYDAVADPMKVESAIVRFRPKMVTMVHCETPSGTLNPVGEVGRLVAKHGV
                     PLFYVDAVSSAVGAELRTDEWQIDLCLVGSQKCLSCPSDLAIVAVSPQAWTVIDEVDY
                     QGYDALLPFRKALENRYFPYTPHWHGIAALEIACRRVLAEGLDAVQARHQRAAEVCRQ
                     GLVDLGLTLYPQSMEFNSPTVTAARVPAGLGWAEFDRRLRRRGVGVGADYGPLEGRVF
                     RLGHMGTQADDGLVREALSAIGES"
BASE COUNT         1783 a         2899 c         3459 g         1940 t
ORIGIN      
        1 ctacctttag accattgatg gagcccatcg actcgggagg atcgccatga agcgactgct
       61 gctgagcctg tctctcgtcg ttctggtagc ggtggtattc ggtgttgccg tcaatgctca
      121 gtccgatgct cagccgaagt cgggtaggac tggatccgag aaggttcctg cgcccgtggt
      181 gaaggcgagt gtgacccgca tagtggatgg ggagtcgatc gaagtggata tccccggccg
      241 gggcggtgtt aaggttcgat atcttgggat cagcactcca gagtcgtccg atccgcgccg
      301 gtcggatgag tacctcgtcg aagtggccac gacccggaac aggggcctcg tcgacgggaa
      361 gacggtgtat ctcgaaaagg acgctaccga cgccgacaag gagggccgcc tactccgcta
      421 cgtttacctg gcggacggta ccttcgtgaa tcagcagttg gcgaaggacg gcttcgctgt
      481 agccaaggtt gttccacccg acgttaagca ccgcgcggcg cttgaggccg ccgagaagga
      541 tgcccgaaac cactcgcgtg ggatgtgggg gccggtggag gcgttcggcc ccatggaaat
      601 cctgcccgtg gtgaacccca ctcccgtgcc gtgagccttc tctgctgtcc gacggacctc
      661 tcaggccgcc tcttcctgga cctgggcctg aatactctcc cagtcaaagg ccagttctgg
      721 aatgggttcc ttggatatcc tttgccagtc ccagccgagg atttccgata ttctttcccg
      781 gttccactgg tcaaacgggt cctgggacct ggagagatgc tcgaacgccc cctgaggatc
      841 cgcaacctcc cagaataccg agaccacgtc tccggtggcg gtgtgttgaa gccaggcaag
      901 ttccctggtg atgccggcct ttccgcggca ctcctcgtac tgctgtctgc gagagcccag
      961 catctcctcg gtcatccgct tccactgatc ggtctttccg ggcaggatct gggcagagaa
     1021 gcaaaacagg gccattctcg ttgcagcctc cagatagagt ccagctctgt ctgagccgta
     1081 ccatcactca ttgccggtgt agtgccagta actcggtcgc aagttctgag cctttctcga
     1141 ctgtccgtgt gccggacaat ttgtgcgtgg ccggtgaaga tttggggcaa gggtagcatg
     1201 cgaagacgga cagggcgctt tctgaccaca gctcaagttg ccgcggcggt gggggtgcat
     1261 ccgaacaccg tgcggctgta tgaggcgtgg gggttcctgc ctccgatccc gcgcggtccc
     1321 aacggctacc gtcgcttcac cgaggcgcac cttgacctga tgcggctggc acgcaccgcc
     1381 ctccaatacc cctacccggg cggcaaggaa ccggtgctgg ggctgctgga aagggccaag
     1441 gccggtgacc tggagggggc gctcgaactg ggcaggggct acctggacca ggtgcgaggc
     1501 gagcgagcga aggccgagga ggccgtggtg atcctggagg gctgggctcg acgtggcatg
     1561 gtatcgcgtg cagggaggct gttgcgcatc ggggaggccg caagtctgtt ggagactaca
     1621 gtcgatgcgc tgcggcactg ggagcggaac ggcctcatcg aagtcccgca ggaccctcgc
     1681 aacagatacc ggggctacgg tccgttggag ttggaccgtc tgcgggtgat ccgtgtgctt
     1741 agaagcgccg gctacagcac gatgtctatc cttcggatgc tgcgccacct ggacgagggc
     1801 agcacagacg acctccgcca tgtccttgat acgccccatc ccgacgatga tgtcatctac
     1861 tggacggatc gctggcttag ctcgcttgcc gagcaggagc gacgggcgat ggacgttatc
     1921 gcgttgctgc acgagatgct cgagaagcgc gggtagcagc cttctgcgca cctcccagac
     1981 cgcccccacc acaaaccctc cattagcgca ccagggttgg agaatcgaat catgagtgat
     2041 ttggtgtgtg gccgggagca tccggccgaa gggagggacg ctgttggaga cgatccacga
     2101 gaccgagacg atgagccacc tgcggcgatt gtgcgagatc ggccccagac ccataggctc
     2161 gaaggcgaac cacgccgcgg cggagtacat ccggggtgtc tttcggtctg ccggactgga
     2221 ggtggtcgat cagaccttcc agtgtccgga ctgggagacg cgggagagtt gggcggagat
     2281 tgaaggactg aggcttcgag ccgaggctaa cgtcttctcc ccctccgtgg atgtcaccgc
     2341 accgacggtg gcgctcgggt ccattccaga gttggagcga tcggaactgc agggccggat
     2401 aggcgttctg tacggtgatc tggctgccag accgttgacg ccgaaggggt gcacgatcta
     2461 ccagcacgag cgcgaccagc gggtcaatcg gctgctggag gagaagaggc cgctggcgtt
     2521 gatcacggtc agtccgcgga taggctccgt cgagagggtg atcgaggact ggtcgctgga
     2581 gatagcctct gcgacggtgc ccccggaggt cggcctgtcg ctgctgcagc ggatcggcga
     2641 tccggtccgg ctgaggatcg tcaccgagag cagtctggga tactcctgca acgtcgtcgc
     2701 ggataggccg ggtgttcgag aggggcgtat cgtgatctgc gcccactacg acaccaagat
     2761 cgagacgccg ggcgccgggg acgacgcggc cgggatcgcc gcagtactga ctctcgcgca
     2821 tctgcttccg gctcgatacc ctggggtggg tctggagttc atcgccttca gcgccgagga
     2881 gtatggggcg agccaggacg acacggagtt ctacgtgcgc tgccgcggcg acgcgatggg
     2941 cgggatcacc gcggcaatca acatggacct cgtcggacaa aggctgggga ctaccgcgat
     3001 ctccatgatg gcgagctcag agacgttccg agatagcgtg gatgctctac tgaaggagcg
     3061 ggcgggcctg atctggacgg agccgtggcc ggagagcaac cactccacct tcgcgctgcg
     3121 gggcgtgccg gccgtcgcca tcaactgcaa ggggatcacc aacctgaccc acatgcctga
     3181 agataccatg gattggatcg gagagaagaa gctgggagag gtggtggaga tcgtctccgc
     3241 catcgtcggg ggccttcagg acaagtcggt cgactggacg agagaggctc ggtgcgacct
     3301 cgatcgaact accgcgatct agagggagga tgggtaggat gttgccaagg gttgtcttgc
     3361 acaatgccgt cagcatcgac ggtcgcatcg acggtttcac gccggatctc gggctttact
     3421 acggactgat cccgcgatgg gcagaggatg ccaccctggt cggaaccgag acggttctgg
     3481 ccgccgacct gggggcgccg gaagaggagg agactgagga tccgccgccc gtttcagcgg
     3541 gcgatcggcg gccgctgctc gtgatcccgg acagtcgggg ccgggtccgc gcctgggaca
     3601 ggttgcgaaa gcagccttac tggagggacg ccgtaagcct ctgctctcgg tccacaccgc
     3661 tcgagcatct agcttatctg tcggagcgga gggtggacca catcgtggca ggggaggaca
     3721 aggtcgactt gcgcgctgcg ctggaggagt tgaacatccg gtacggcgtg aaggtcgtca
     3781 gggccgacac gggcggcatg ttgaatggag cgctgctcca ggccgggctg gtggatgagg
     3841 tcagcctgct gatcgccccc tgtctggtcg ggagttcaac cccaaagtcc atattccagt
     3901 tgccggacga gttgaagaag ggcatagaga tcccagcgcg gctgatgcac ttggagcgga
     3961 tggccggcga ctgggtgtgg ctgcggtacg agttggcgaa gtagtgtgga aacccgcaag
     4021 tgctagactc gttaggccag caccgccttg atcacgcggc aggctctcag ggtaacccac
     4081 ttgctgggtt cgcccagccg ctcgaggtcc acccaggtct tgccgttgta tgtatacctg
     4141 ttcctccaac gcccgtcgga gtcctgctgt tccagcagcc actgaacggc gcgctggagc
     4201 cgggggtccc gagccatgcc gtgttcgcac agcgcttcga ggatctgcag aaggtccgtc
     4261 acgtagccca ggggaaagcc tagcttgaac caggaccggc tcggcttctc tccgtgggcc
     4321 gtcgggtagg ccgcagtcgc cgggtcgcag ctgaggagga agtcgctccc ctgctcgatc
     4381 gccctgcgga ccgcagtcga tcgtcgttcc tccgggacgc gggccagggc cagcatcgcc
     4441 ttgacggccc cccatgcgca cggcttcccg ttgttggctg cacagcagaa gccggggccg
     4501 ctagtcgacg acttgtagaa gcggtcgaag ccttcgccgg tgattgccct cgcctgccag
     4561 tcgatggctc gctgcactcg agggtcttcc tgccggccga agccgatcag cgcgcggagg
     4621 aggttgccgt ggagacaatg gatcacggca gacggcggcg gagccttctc ctcgctgctg
     4681 gccgaacagc cgaaccctcc gctgctggta gggcagacgg tcaggacgta gtcgcaggcc
     4741 gcccggattc gggcgtccga gctgtcggct cccatctgat ccaggaagat gacctgccag
     4801 acggttccca tgtacttagg gccatagccc ggcccgggct tgacccagta gccacgtgga
     4861 tcctgggcgg cgagaatcgc tgcgatgggg ttgctcttca gagcatcgcc gcgtgcacga
     4921 attacctcag ggtcgtcctc aggtcggtcc agtaggaatc gcagcgccat ctgtcgcacc
     4981 gcggggttgc cctcttccag cagccaggga aggggattgc ctctgagcgt gctcacccat
     5041 ccggtttcca tctttgtctc cgtcactcgt gccggccaac tctgtcagcc gcggctctca
     5101 tcggaccggt cgctgggaaa ggcgactctc ctgtgaagaa gtcaccgggg ggaagaatcc
     5161 gtgaggagca tgatactcct tggagtagat gatctgccac aaggagtatc gacagtcgcg
     5221 tcgcctctac agagcggcgg cctcgttctg gagttgttcc agggcagcgc gcatctcggg
     5281 caattcgcgg atcttcagga tctgcagcat cttctctcga ctgaggccca gcgaggatgc
     5341 gcgaggaggg atccagcgcc tgaagatggc aacggcgtga agctggccat agtgaaggat
     5401 ggatcgttcg aactgttccc gcaagatggc caccttctcg gcctgggagg gggctggcag
     5461 tgcagacagt ctggaaagct ccctccagat gttccggatc agccaggggt cttttgtggc
     5521 gccacgggcg accattacgc cggcgcagcc ggtttcctca agcatgcgac gggcagcaag
     5581 tggggatgtg atgtcgccgt ttcctatcac agggatcccc tctacggctt ccaccacctt
     5641 cctgatctcg tccaggtctg cccgaccccc gtagccctgc tgtcttgttc tgccgtgaac
     5701 cgtgatggct gcgatcccga cgccctccaa ttgccgggcg agatccgcgg cgactatcga
     5761 ctccgcatcc caaccgagcc gtatcttggc ggtgacgggt accgagacgg cacgcaccac
     5821 ctgctccgcg atcctgacgg cttccttcgg ctgcttcagt agcgcggcac cggcgttgcc
     5881 ggcggttatc tcccgctggg gacagcccat gttgatatcg acgaggtcgg tcccttgctc
     5941 ctccagccac tgagctgccc tcgccatcag gtcggcgtcc gtcccgtaga tctgatgccc
     6001 caggggggtg tcctcagggg tcgtggcgag cagttcggtg cggcgcttgc cttttccgaa
     6061 gagaatgctg ggcgggttca tcatttcggc gaaggcgaga cccaatccac ctagcgggcg
     6121 gacgatggac cggaagggca ggtctgtgta gccggccatg ggggccagaa ggatgggagt
     6181 ctggaggtga aggctgccaa tccgcagcgt ccggctatcg gagacttgca ttagtgatcg
     6241 cgtgtggagc tactgggcgt ccttgtgaag cggttacacc gcggctcagt gcacaacttt
     6301 tcggctggag ttgacattct gaacgggaag ggctatcgtc aaaaagaatg accctatagc
     6361 ctgccatcag tatcataatg tttattgaat ggtagcatag ccggcgcaat aatcgcaata
     6421 gtgctggctg ttggcgcttt ggaggttaga ggtgagcaac cagttcgagt tggtggtcga
     6481 ggagcgatcg gtcctcggca agcaggtcaa gaggctgcgc cgggacggga tcgttcccgc
     6541 caatctgtac gggcacaggg agccatcatt gccgctgcag gtgccaagcg cagtgctcag
     6601 ccggctattc agccagggtg gagccacgac gcttgtccag ttgaagatcg gggctaagcc
     6661 accggttcag gcgctcgtga aagaggtgca gcacgatcct acgaccggct cggtgaagca
     6721 cgtggatttc ttccgagtcg ctgccacgga gaagttcagg acgcacgtcc cgctgcactt
     6781 catcaatgag gcggaggcga tgaagctggg ggatgtcgcg gtgcaaaggt cgctcgcgga
     6841 cgttctggtg gaatgcctgc ctgccgattt gccggccgcg atcacggttg acctggcaaa
     6901 acttggtgag attggcgcag tcatccgagt gggcgaactg gatgtgcccg agggcgtctc
     6961 cgtgcttctc gatcacgaag aggtggtcgc gggggtgcac cgcgtggcga gagaagcaga
     7021 acccgaggtg gaggctgctg cgccggccga gggtgctccc gctccgactg aagagggaga
     7081 aggcgcatag acctggccga gaagtggccg agtcaactga agttgtgatc tgaacgccga
     7141 tatggagtgc ggtgcctttg catcgcactc catatgcagt taagaagctt gtgcttctag
     7201 tgctcgacca cagtgatcct gacggtgtca tcctgagcga cagcgaagga tctctccgtt
     7261 cacgaggaga tgcttcgctt cgctcagcat gacaatgcgc gtcacctgta ctgtgtcaga
     7321 gtactaagta cgtcgtagaa agaaacgggt ccagggcgta gcgcctggcg gggtttgggg
     7381 tgtccccaaa gttacagcct ctttctacta cctacttagg gtgcgggcga gtaagatgac
     7441 gccgctgtcc cggcaaaatc agtaaagggt agctctcttg actcatgtag tggacgatta
     7501 cgacatcctg cgcacagagg ggctcagccg ttctgtggcc ggaaagaccc tggttgacgg
     7561 gatcaccgtg ggcatgaagc agggagatgt cctggccgtg gtgggcccca gcggggccgg
     7621 gaagtcttcg ttcttgagat tgctgaaccg actggacgag cccaccgagg gtacggtgta
     7681 tctggatggc cgggactacc gcaccatctc ccctcgagag ttgcgccgga gggtgggaat
     7741 ggtgatgcag tcgcccttcc tctttcccgg cacggtggga gacaacctgg tgttcggtcc
     7801 cagacagtgg ggggaatcgc ccacagagga gacggtggaa gggtggttgg cgcgggtggg
     7861 ccttgccgga tacgtcggcc gcaacgttgc cacgctatcc ggcggggagg cgcagcgggt
     7921 atccctcgcc cgtaccctgg ccaactcacc cgaggtgctg cttctggacg agcccacctc
     7981 ggcgctggac gaggccgcca aggccgggat cgaggcgctg atccgagaca ttttggccga
     8041 cggacggctg acatgcgtga tggttactca cgacatggct caagccgagc gtatggcgaa
     8101 ccgggtcatg gtgctggagg gagggcgagt ggccagggtt gggccggtcg aggaggtgat
     8161 ccgtgctcac tcaagtcttc actaaccccg tgagtctcgg tcttgcacag gccgcagtgg
     8221 tcagcttctt gtccatcgcg gtggtgttga tggcaagccg gcgagggatt cgcgtcgagc
     8281 gcgagacgct agtcgcgttg gctcgaggcc ttgtgcagat cgttgcggtc ggatcggtgt
     8341 tggtggtgct gctgaatgga ccgatctgga cgagcatcat agtcctttgc gccatggctg
     8401 tggcagccgc ttctacctct gcacggcgag cgaagaaggt gcggggtgct ttctgggatt
     8461 ccctctgggg ggttgggttg ggcgccggct cggtgatcgc cgtgatgacg ctggtggggg
     8521 tgatcgagcc cggcgtagac tccgtggtcc cagtcggtag catgatcatc gccaatgcca
     8581 tgaactcggt tgccattgcc atggaccgct tccgctcgga cgtcgaggcc cacacgggcc
     8641 agatcgaggc gggacttgcc ctaggggccg atcccaaaac ggtggtggcg ccctacgttc
     8701 agtcgtctgt gcaggcgagc atgatcccac gaatcgacaa cttgcgctcg ttgggtatcg
     8761 tgtggatacc ggggctgatg gccggtatga tcctctccgg gagcgatccc gtctacgctt
     8821 cgatctacca gttcgtggtg ctgggcatga tcttcgcgtc ggctggactg acctccgtga
     8881 tcagtctgat gctctttcgg aggcgggcat tctctaccgc ggagcagctg gtgatgagac
     8941 ccagtgcaag cgtcggcggc ggttcgtcga gtcgttagcg aaacaccact tcttgtgagg
     9001 gagtacagat gcgcagctac cgcgtcccca tggttcctgg cccaacgagg gtgccggccg
     9061 acgtgtcggc cgcctatcag gccgaatacg gctcgagcga cctggaagag gagttcttcg
     9121 acctgtatgg ccgggtgcag gagcaattgc agcagctact gatgaccagg agccagatgg
     9181 ccatcatgac cggcgagggc atggctgcgc tctggggtgc cttgaagagc acgatccggc
     9241 cgggcgaccg ggtgctatct gtcgctaccg ggttgtttgg ctacggcata ggggagatgg
     9301 cccgggccgt gggcgctcac gtggaggtag tggagtttcc ctacgacgcg gtggcagatc
     9361 cgatgaaggt ggagtcggcc atcgtccgtt tccggccgaa gatggtgacg atggtccact
     9421 gtgaaacgcc gtcgggcacg ctgaatccag tcggggaggt cggccggctg gtggcgaagc
     9481 acggcgtgcc gctcttctac gtggatgcgg tgtccagtgc ggtcggcgcg gagttgcgca
     9541 ccgacgagtg gcagatcgat ctctgcctgg tggggagcca gaaatgcctc tcctgccctt
     9601 cggacctggc gatcgttgcc gtgagccccc aggcatggac cgtgatcgac gaggtcgact
     9661 accagggtta cgatgcgctg cttcccttca ggaaggcttt ggagaaccgc tacttcccat
     9721 acacgccgca ctggcatggt atagcggcgc tggagatcgc ctgccgccgg gtattggccg
     9781 aggggttgga cgcggtacag gctcggcacc aacgggcggc agaggtctgc cgccaggggc
     9841 tggtggatct gggattaacg ctgtacccgc agagcatgga gttcaactca cctacggtca
     9901 cggccgctcg ggttcccgcg ggactgggtt gggccgagtt cgaccgacgg cttcggcgtc
     9961 ggggcgtggg tgtcggggca gactacggcc cgctggaagg cagggtgttc cggctgggcc
    10021 acatgggcac gcaggccgat gacgggctgg tgcgggaagc gctgagcgcc atcggggagt
    10081 c
//
LOCUS       DATALS010000013        12223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_90894,
            whole genome shotgun sequence.
ACCESSION   DATALS010000013 DATALS010000000
VERSION     DATALS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 12223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12223
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90894"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(441..3311)
                     /locus_tag="VHS06_00300"
     CDS             complement(441..3311)
                     /locus_tag="VHS06_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HEX2986607.1"
                     /translation="MAVADRAKTQNGVSSVSRPLVFRFALNWEAIAYAVIALIAVATR
                     FWDLGSRAIHHDESLHALYSWYLYIGRGYVHDPMMHGPFQFFGNAFFYLLFGASDYTA
                     RMLAATSGVWLVLAPYFLRREMGRLAAIVASILFAFSPVFLYFSRFTREDIYMAAWEM
                     VMVVGLFGYLRTKAPRWLYTAVIGLGFAFATKESVYITGFIFVMAFVIEAGFALGRGR
                     SPRGWTLLASLSPRDWVVTVAIFFTIYVLLFTTFFTNPKGIISGSIGALQYWFAQHDV
                     QRGGQPAFYYLMLLPLYEFLPLSAAIAGLVVAAVQRTLSRQNFFVWFMAIWFLSALVI
                     YSYAGEKMPWIMTHITQPLIFLAAVSVAGLLRRLNRSLLRSGPALLALCLTGILAATL
                     LGGMVAAEPVTGTQLGLQAAQLQRLAMWLLVVVLLAGLVGIGVRYGSRLVLVPAGLGF
                     AVALFLLSVHTAWGVAYARGDVPQDMLVYVQTSPDVPRFVQEIDRISYQTGAGKDMKI
                     LLDGGYNDETGQHESIAWPFEWYLRDYKARTYYSKTLPTPADAPVILAMSSNEEPIRN
                     QLSNYVAVHGRLNWWYPEDYKTLTWEKVWQGLQDASVRAKLWRYFFYRETLNPLGARE
                     IDFFVRSDLARGIPLQMGAQPPAPSVPSAPAAQIGDGVAQAAPGGLTIYGKSATGTNL
                     LAEPRGIALATDGTLYVVDSFASKVTAFRPDGTIAYQWGKKGSGDGEFSEPWGIAVAP
                     DGDVYVADTWNHRVQHFDRTGKFISKWGGFVDAKGQLGVQPGSFWGPRDLVVTSAGNV
                     IVSDAGNKRVQVFDRDGKFITMFGGDGTEAGKLKEPVGIAADGAGNIYVADTWNRRIQ
                     KFDSQFRPVAQIPVSGWDSQSVVNKPYLAVSEDGHIFYTVPEKNQFVVVTPDGKQEAA
                     RGAQGSDPQSFNLPIGIAVSRQGEVFISDTANTRVVKYSSWR"
     gene            complement(3335..5026)
                     /locus_tag="VHS06_00305"
     CDS             complement(3335..5026)
                     /locus_tag="VHS06_00305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2986608.1"
                     /translation="MDGDIGVSRTDTGWYPTIETVLFGSAILAGILLRFLLLDARPLT
                     VDEGGLASQAFRVMLGQFGEAFEDGPLPTYGTATFLALFAAGDGAARLLSVMSGIVLT
                     SVPYLLRGSLGRGTAIAAAWGFAVSPLLLFASRTVGSGVLPAALAILLWWLLETSEDS
                     DYWRPFAIAGLLAALVSCGPTGLTCIVATGVAFLLSHPAPDILVRDIRYAAASTTGRQ
                     AVILFAVSSVALSTGLGANLRGIQYLVVDLWVGWLGTFNPLAPRGGLLLLLAMYELPV
                     LLFAIGRLLYALAHRDRVDMFLSLWAMLLLLAGMFNPAVGLPKIVVALVPIYLLAART
                     VSGTVHLARGAAIEWRWVGGFILVGVPIVVAMILLNRSSVLLEPIPQEYVIGEVSLVL
                     CAVIAAFTLLDGGGRKAVAWYVVAVAAFGLWIHTAAFLNYRTDTFQEEVVAGQQLSTQ
                     LRDAAVEGAYYSTRFNARLEVAPQLKTPLEWYLRNASNVDYAAGSTDGPSIVFADPGS
                     GIATAGSERAPGLYSPSFDPAGFTWQGAWGWTMARSGLVRPNPRDIILRIPLSVW"
     gene            complement(5143..7401)
                     /locus_tag="VHS06_00310"
     CDS             complement(5143..7401)
                     /locus_tag="VHS06_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX2986609.1"
                     /translation="MIWFLSWLLAVEAAGVAALPLSVRLLRALPDRGFFFSKPLGILL
                     IGYLCWLPSVLGMLRFQQSTVVAVLVIVAASSWMAWGREAFKQLSTARFSLVFSELTF
                     LLALGIAAAIRASFPDISGTEKPMDFAFLNAVARTETLPPEDPWLSGFGISYYYFGYY
                     LLGMLVKLSGIQASVGYNLSVALVFAMLVAGSYSIAFNLLSSLRPDWPLPSRLAGAVL
                     APVLVAVMGNLEIALEVLASKGYSNPSFWQWAGIKNLHPSPTPGGPFPTDFWWWWRAS
                     RVIPTTTPDGINEFPFFSFLLGDLHPHFTALPWVLLTVATSFAAMMKRVAGSMTDGWW
                     PTKVVSALILGFPLVGNSWDYPTYAGLYIAACLVPFIRRGWKRESLADGIKLALPVIA
                     ASIAMYSPFFLGFSSQTRGLGLSADRTPLVSMLIIFGALLSGIVVLLSWRGWTGKHDP
                     GIRTSPPRTLVVAGLLLLLAAPVGGSLALLAGVLLLGLSAVVRFYSDSEPPAPARGAQ
                     LFLLLLAALGLALIVIPEFVFLMDLFGTRMNTVFKFHYQAWLLLGIGSGVGVVWTVGT
                     TRRRVAAVAAITPFALMVGIGLLYPVGATASRTQDSSTQATLDGAAFFQVQRPDEYSA
                     IQWLARNASGRPVVMEATGGSYSSYARVSTFSGLPTVLGWASHEVQWGRNGAELQRRT
                     QDIDAVYSTASRDSIVAILRKYQVVYLFVGSLEVEKYGPALSERFSGLLEPAYRQGNV
                     VIYRVPPAGRSG"
     gene            complement(7415..12010)
                     /locus_tag="VHS06_00315"
     CDS             complement(7415..12010)
                     /locus_tag="VHS06_00315"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX2986610.1"
                     /translation="MSDSTLRQGRGPLAAVATLLCLTAILAVAAFFRLTGVNWDSFTH
                     LHPDERFITMVENSLDWPTSLGQYFDTARSPLNPYNRGYNSFVYGTFPLFLVKWLGIV
                     LNMSGYDEIHLVGRVVSALFDIGSVLMIFFIGRRLFGSRSGLLASLLSAGAVIQIQQS
                     HFFTFDTFVGFFLLVSFFFAVRIWESPHAYDYPLLGASLGFAMACKINSAVFAIVVAL
                     IAVKQLREVTLVFPNQRMADWMAVIGRFAGVALVALLVFRVVEPYAFAGPGFFNIGLS
                     TKFLNDIGFVQKLVSGEVDQPPSVQWANTPAYIFPLRNLVFWGMGLPLGVVGWVGLVM
                     GAFLLLFRRETKYLLLVAWVGFFFLYQGGQFAKTMRYFLPIVPLLSLLGAQMLVAGFD
                     RARERVWTSDRRATLARAATYALPAVTLAVAVCTLAYAYAFTQIYTRPVTRIEATEWI
                     FANIPQGTTIANEHWDDPLPLRWKGRDAGWYSGTMLELYNDDTPEKYNKLVQQLDTTE
                     YIFISSNRLYGSIPRMPLKYPMTTEYYRLLFSGQLGFRLEKTFTSHPTIFGIEINDDS
                     AEEIFSVYDHPKVLIFHKTADYSPAKTRQLLGAVPLDSVVQQKSVEAQYGGLLLSSAD
                     RDAAQAGGTWSEIYDVNSLANRVPVLAWLLLVEVLGLLALPITWLLFRRFPDRGYGFA
                     KAIGIAVTAYVAWIIPSFRLAAFGPMTALAGTALLAGIGAVALRGRAREFWGFARQGG
                     RLILAEELVFLAAFGLFLAIRMSNPDLWHPSFGGEKPMEFAYLNAIAKTTYFPPYDPW
                     FAGGYINYYYFGYVIVATLMRLTGIVPAVAFNLAIPTIYGLTAVGLFSFALNFAAVRQ
                     IGARLLSLGNVATGLAATVFVLVAGNVDGLIQRLESLWALGGSQFRSTIPGLEGVVKA
                     GYGLSAVLLGGKTLPPLDFWRSTRLIPGENPTPIMEFPWFTFLYGDLHAHMIAMPLTV
                     LALGLTLAIVWQAGSRRPADKENAERLAGLPQTKIVVAGMVGQRDGAVTGDSLAGRDL
                     PIAGGEEATIPEAPRGWSLAERGLSVLFAGFVVGMLQATNSWDFPTYLILISASFLVA
                     SYARDRAITIDGLIWAAGSAGFTYVVAMILFSPYTRNYELFYNGVDPAPAKTDFRYYL
                     VMFGLFLFCISTALIYAVVKDRWLRFQWSEAYERFTGLARYDRREELWRVLVRPSPWV
                     ELLPWMVAALMAVILIALVARQYLVALLIGLCLLALLSLFRRDASPEELLLSLFVGLG
                     TVLTLGVEFITIKGDIGRMNTVFKFYLQAWFLLGLASALLVAAMLRHWYRGGHLKRFG
                     WRFAWIAAGVLLVGSTMIYPWSATPSKVSLRFEQTPPSLDGMAYMQGAVWKDQNKDIV
                     LAHDYEAIRWLQDHVAGSPVVLEAQIPEYRWGSRVSIYTGLPTILGWTWHQRQQRGNY
                     GAMIDQRLIDIKTMYDGQDLAATSSLLRKYGVGLIYVGDLERAYYSAAGLAKFDKLVA
                     NGGLAVDYRADGVTIYEVKREDRAPLLISSATRPGAG"
     gene            complement(12003..>12223)
                     /locus_tag="VHS06_00320"
     CDS             complement(12003..>12223)
                     /locus_tag="VHS06_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986611.1"
                     /translation="RAFDLSHYLSAPSVIVRRLTGRWILFPNKPTAIRRSLFSRFYEQ
                     DPEGEGAYIFLVCRKSQPASVGVEEAGV"
BASE COUNT         2786 a         3685 c         3679 g         2073 t
ORIGIN      
        1 tctggggtcc acgtgatctg gtggtcacgt ccgcgggaaa tgtgatcgtg agcgacgccg
       61 ggaataagcg cgtccaggtc ttcgaccgcg acggcaaatt catcccatag gttttgcggg
      121 cacccgtacg gcgcctgaga agataggctc gcacgacctc ccccagcctt gccgggagca
      181 agttgttagc cgcgaacccc atcatcagga tgccgaacgc acctgaggcg gatactggtg
      241 cgaccgggcg gacgatgcgc tgccaacgga gactgcggat gacgtagccg caaagtgtcg
      301 cgagcgcggc cggtgccacc aaacgatagt cggctccggc gagggcagcc gcgatctcag
      361 aaccgtttac gttgcgagct atgacccaca ggaacactcc tgtgagtacg aatccgacta
      421 ctcgaccttt tcgaaacaac ctacctccag gatgagtact tcacgactct ggtattcgct
      481 gtgtcagaga tgaacacctc gccctgcctg gagacggcaa tcccgattgg taagttgaag
      541 gactgcgggt cggaaccctg ggcgccgcga gcagcctcct gcttgccatc tggcgtgaca
      601 actacaaact ggttcttctc gggaaccgtg tagaaaatat gcccatcttc gctaaccgcc
      661 agatagggct tgttgaccac ggactgacta tcccagccgg aaacaggaat ttgcgcgacg
      721 gggcggaact gggaatcgaa cttctggatg cggcggttcc aggtgtccgc gacgtagatg
      781 ttaccggcgc cgtctgccgc gatcccaacg ggttccttga gtttgccagc ctcggtgccg
      841 tccccgccaa acatggtgat gaatttgccg tcgcggtcga agacctggac gcgcttattc
      901 ccggcgtcgc tcacgatcac atttcccgcg gacgtgacca ccagatcacg tggaccccag
      961 aaactgccgg gctgcactcc caattggccc ttcgcgtcca cgaacccgcc ccacttggag
     1021 atgaatttcc cggtacgatc gaagtgctgc acccggtgat tccaggtatc ggcaacgtac
     1081 acatcaccgt ccggggccac cgcgataccc caaggctcgc tgaactcacc gtctccgcta
     1141 cccttctttc cccattgata ggctatggtc ccgtcgggcc ggaaggcggt tactttcgat
     1201 gcaaacgagt cgacgacata gagcgttccg tccgtggcga gggcaatgcc acgaggttca
     1261 gccagcaggt tcgttccagt tgcgctcttg ccgtagattg tgagtccgcc cggggctgcc
     1321 tgggccacgc cgtcaccgat ctgtgcagca ggagcgctgg gaacggatgg cgctggcggc
     1381 tgagcgccca tctgtaatgg tataccccgc gcgagatcag accgaacgaa gaaatctatc
     1441 tctcgagccc ccagaggatt cagcgtctcc cgatagaaga agtaccgcca cagcttcgcg
     1501 cgcacggaag cgtcctgtaa gccctgccag actttttccc aggtcagagt cttgtagtcc
     1561 tctggatacc accagttgag cctgccatgg acggcgacgt agttggaaag ctgattccgg
     1621 attggctcct cgttgctcga catggccaag atcaccggcg cgtccgctgg tgtaggaagg
     1681 gtctttgagt agtaagttcg agctttgtag tcgcgaaggt accactcgaa cggccacgcg
     1741 atgctctcgt gttggccggt ctcgtcgttg taaccgccat ccagcaggat cttcatgtcc
     1801 ttccccgcgc ccgtctgata ggagatccgg tcgatctctt gcacgaagcg aggaacgtcg
     1861 ggagaggtct ggacgtagac gagcatgtcc tggggaacgt cgccacgcgc gtaagctact
     1921 ccccatgcgg tgtggacaga caagaggaag agggccacgg cgaacccaag tccggccgga
     1981 acgagaacaa gacgagatcc gtagcggaca ccgatgccta cgagacccgc caacaagacg
     2041 acgacgagca accacatggc cagccgctgc agttgagcgg cctgtagtcc cagttgtgtt
     2101 ccagtgaccg gttccgcggc gaccatgcct ccgagcaacg tggccgcgag gatgcctgtc
     2161 aggcagagcg ccagcaaggc aggaccagat ctcaggagcg atctattcag ccgcctgagc
     2221 aatccggcta cagatacggc ggcgagaaag atgaggggct gcgtgatatg ggtcatgatc
     2281 cacggcatct tctcgccggc ataggagtag atgaccaggg ccgacaagaa ccagatcgcc
     2341 atgaaccaca cgaagaagtt ctgcctcgac agcgtgcgct gcacggccgc gacaacgagt
     2401 ccggcgatcg cggcgctgag agggaggaac tcgtacaagg gcagcagcat aaggtagtag
     2461 aaggccggct ggccgccgcg ctggacatcg tgctgggcga accagtattg cagagccccg
     2521 atgctaccgc tgattatccc cttgggattg gtgaagaaag tggtgaacag cagaacatag
     2581 atggtgaaga agatcgctac ggtcaccacc cagtcgcggg gcgaaagcga ggccaatagc
     2641 gtccatcctc gagggcttcg ccctcgcccg agggcgaagc ctgcctcgat gacgaaggcc
     2701 atcacgaaga tgaagccggt gatgtaaacg gactccttgg tcgcgaacgc aaaacccagt
     2761 ccgatgaccg ccgtatacag ccaccgcggg gctttggtcc gcaggtaccc gaacaacccc
     2821 acaaccatca ccatttccca ggccgccatg tagatgtcct cgcgggtaaa cctggaaaag
     2881 tagagaaaca cgggggagaa ggcaaacagg atgcttgcca cgattgccgc gaggcggccc
     2941 atttcccttc taaggaagta cggggccagg acaagccata caccggatgt ggccgccagc
     3001 atgcgtgctg tatagtcact ggctccgaac agcaggtaaa agaaggcgtt gccaaagaac
     3061 tggaaggggc cgtgcatcat cgggtcatgc acgtaaccac ggcctatgta caggtaccag
     3121 gagtagagag cgtggaggct ctcgtcatgg tgtatcgccc tggatcctaa gtcccagaac
     3181 cgagttgcca cggcgatcaa ggcaataacg gcgtatgcta tcgcctccca gttgagggca
     3241 aaccggaaga ccaacggtcg gcttacgctg ctgactccgt tctgagtctt tgcccggtca
     3301 gcgactgcca aagggtacga cctccttcta ttgcctacca aacactcaaa ggaattcgca
     3361 ggattatatc acgggggttg ggccgaacca ggccgcttcg agccatcgtc catccccagg
     3421 caccctgcca tgtgaacccg gcggggtcaa aggacgggga gtaaagtcct ggcgctcgct
     3481 ccgagcctgc ggtggcgata ccggaaccag ggtccgcgaa aacgatgctc gggccatcgg
     3541 tgctgccggc cgcatagtcc acgttggagg cattcctgag gtaccactca aggggagtct
     3601 tgagttgggg cgctacctcg aggcgagcgt tgaagcgggt agagtagtat gcgccctcga
     3661 cagccgcgtc tctcaattga gtggatagct gttggccagc gaccacctcc tcctgaaagg
     3721 tgtcggtcct gtagttcagg aaggcagccg tgtgaatcca gaggccaaaa gcagctacag
     3781 caacaacgta ccaggccacg gcctttctcc caccaccatc tagcaaggtg aaggcagcaa
     3841 tgacagcaca cagcacaagg gagacttcgc ctatcacgta ctcttgggga attggctcta
     3901 agaggactga cgagcgattg agtaggatca tcgcgactac gattggcact cccaccagga
     3961 taaagccgcc tacccaccgc cattcgatgg ccgcgcccct tgccaggtga accgtgccgg
     4021 acaccgttcg ggccgcaagc agatagattg gcacgagcgc gaccacgatc tttggaaggc
     4081 ccacggccgg gttgaacatg ccggcgagta gaaggagcat cgcccagagt gagaggaaca
     4141 tgtcaacacg atctcgatgg gcgagcgcat atagcagcct tccgatggca aataggagca
     4201 ccggcagttc gtacatcgcc agcagaagca ggaggcctcc tcgaggcgcg agggggttga
     4261 atgtcccgag ccatcctacc cagagatcaa ccacgaggta ctggattccc cgaaggttgg
     4321 cgccaagccc tgtgctcagc gcgacagaac tcacggcgaa caggatgacg gcctgccggc
     4381 cagtagtcga cgcggcagcg tagcgaatgt ctcttaccag gatatccgga gcgggatgtg
     4441 acagaaggaa tgcgacgccg gtggccacga tgcaggtaag cccggtcggc ccgcacgaca
     4501 ccaacgcggc gagtaacccg gcgatcgcga atggtcgcca gtagtcggaa tcctcgctcg
     4561 tctccagcag ccaccacagc agaatggcaa gcgccgctgg aagcacacca cttcccacgg
     4621 ttctcgaggc aaacaagagc aggggcgaga ctgcaaaccc ccacgctgcg gcgatcgcgg
     4681 tccctcttcc gaggcttccc cgaaggagat agggaactga tgtcaggact atgccactca
     4741 tcacagacag caaccgggcg gcgccgtctc ccgccgcgaa cagcgcgagg aaggtcgccg
     4801 ttccatatgt tggtagtggg ccgtcctcaa aggcctcacc gaactggcca agcatgactc
     4861 ggaacgcctg ggaggccagt cctccctcgt ccacagtgag cggccttgcg tccaacaaga
     4921 ggaatctgag gagaattccc gccagaatgg cgctcccgaa cagcacggtc tcaattgtcg
     4981 ggtaccatcc tgtgtccgtg cgcgaaaccc cgatgtcgcc gtccatttcc gccgggggga
     5041 gcgctggacg gggactcctc tcatatttcg atgtcggcga tgtcgtcttc cctggcattc
     5101 tcacctaacc cgtgggccag cagtcaagtc tcaatcttac gcctagcctg agcgccccgc
     5161 cgggggaacg cgatagatga ccacattccc ctgcctgtag gcaggctcca gcaagcccga
     5221 gaagcgctcg gacagtgcgg ggccgtactt ctctacctct agcgacccta cgaacagata
     5281 cactacctgg tacttgcgca ggatggcgac gatggagtcg cgacttgccg tcgagtagac
     5341 ggcgtctatg tcctgggtcc gacgctgcaa ctcggcccca ttgcggcccc actggacctc
     5401 gtggctcgcc caacccaaca cagtcggaag tccggagaaa gtcgagactc ttgcatagga
     5461 agagtaggaa ccccctgtgg cttccatgac cacgggacgg ccagacgcgt ttctagccag
     5521 ccattgaata gccgagtact catcggggcg ctgaacctgg aagaaagcgg cgccgtctag
     5581 cgtggcctga gtgctggagt cctgagtccg agaggctgtc gctcccacag gatacaatag
     5641 gcctattccc accatcaagg caaacggagt aatcgccgcg accgccgcca cacggcggcg
     5701 cgtagttccc accgtccaga caactcccac tccgctgccg attccaagca acagccatgc
     5761 ctggtaatgg aacttgaaga ccgtgttcat ccgggttccg aaaaggtcca tcaggaacac
     5821 gaattccggg atcactatca gggccagtcc cagggcggcc aacagcagaa ggaatagctg
     5881 cgcaccacga gcgggtgcgg gaggctcaga gtccgagtag aagcgtacga cggcggacag
     5941 gccgagcaga agcacgcccg cgagaagcgc aagcgatcct cctaccggcg cggcgagaag
     6001 cagcaagagc cccgcaacga ccagcgtcct ggggggagag gttctaatgc cggggtcgtg
     6061 cttccctgtc cagcctctcc aggagagcaa gacgactatt ccggacagta acgccccgaa
     6121 aatgatcaac atggacacga gcggagtccg gtccgccgac agaccaagcc ccctggtctg
     6181 agacgaaaag cccagaaaga atggagagta catggcgatg gaggccgcga tgaccgggag
     6241 cgcaagcttg attccatcgg cgagagactc tctcttccac ccacgacgaa tgaagggcac
     6301 cagacatgcg gcgatgtaga gccccgcgta tgtgggatag tcccaactgt taccaaccag
     6361 cgggaacccc aatatcagcg cagacaccac tttggtcggc caccatccgt ccgtcatcga
     6421 gcccgctact cgcttcatca tggccgcgaa cgaggtcgct accgtcaaca acacccatgg
     6481 aagggcagtg aagtgcgggt gcagatcacc gagcaggaag ctgaagaacg gaaactcgtt
     6541 gattccatcg ggagtcgtcg taggaatgac gcgcgacgcc cgccaccacc accagaaatc
     6601 ggtcgggaac ggcccccctg gagtcgggct cggatgcaag ttctttatgc cagcccactg
     6661 ccagaacgac gggttcgagt accctttgct cgccagaacc tcgagcgcga tttccagatt
     6721 ccccatcacc gctactaaca ccggcgcaag tactgcaccc gcgagcctgc ttgggagagg
     6781 ccaatcgggg cgaagtgacg agagtagatt gaaggcgatt gagtagctgc ctgccaccag
     6841 catcgcgaag accagcgcga ccgacagatt gtaacctacc gacgcctgga tgcctgaaag
     6901 cttgaccagc atgccgagca ggtagtaccc aaagtagtag tatgaaatgc caaacccgga
     6961 caaccacggg tcttcaggtg gaagagtttc ggtgcgcgcg accgcgttga ggaaggcgaa
     7021 gtccatcggc ttctcggtgc cagagatgtc tgggaacgac gcacggatgg cggcggcgat
     7081 tcccagggca agaaggaagg tcaattccga gaagacaagg gagaaccgcg ccgtggagag
     7141 ctgtttgaac gcttcccttc cccacgccat ccaggaggac gccgcgacaa tcaccaggac
     7201 cgcgaccacg gtagactgct ggaaccggag catgcccagg acgctgggga gccagcacag
     7261 gtatccgatc agcagaatgc cgaggggttt ggagaagaag aagcctctgt ctggcagcgc
     7321 tcgcaggagc cggacggaca gagggagagc cgctactcct gccgcttcaa cggccaggag
     7381 ccaggacaag aaccagatca ttatcgcgcc tgccctagcc ggcgcccggc cgggttgctg
     7441 aggagattag tagcggcgca cggtcctcgc gcttcacctc atagatggta accccgtccg
     7501 cgcgatagtc cactgccagg ccgccgttcg caacaagctt gtcgaacttc gcaagtccag
     7561 ctgccgagta gtatgcacgc tcgaggtcgc cgacgtagat caagccgacg ccatacttcc
     7621 ggagcagaga ggatgtggcg gcgaggtcct gtccatcata catcgtcttt atgtctatca
     7681 ggcgctggtc gatcatcgct ccgtagttgc cgcgctgctg gcgttggtgc catgtccagc
     7741 ccaatatggt tggcaagcca gtgtaaattg agactcgaga cccccacctg tactccggta
     7801 tttgggcctc aaggactacg ggagacccgg ccacgtgatc ctgcagccag cgaatcgcct
     7861 cgtaatcgtg ggccagtact atgtccttgt tctgatcctt ccacacggcg ccctgcatgt
     7921 aggccattcc gtccagagac ggaggagtct gctcgaaacg cagactcact ttggatgggg
     7981 tcgcactcca tggatagatc atcgtcgacc ccacgagaag aacgcccgcg gctatccagg
     8041 cgaacctcca tccaaaacgt ttgagatgac ccccgcggta ccagtgccgc agcatcgcgg
     8101 cgacaagcag agcggatgcc agaccaagaa ggaaccaggc ctgcaggtag aacttgaaca
     8161 ccgtgttcat acgtccgatg tcgcccttga tggtaatgaa ctcgaccccc aacgtgagca
     8221 cggtaccaag cccgacgaag aggctcagca ggagttcctc tggactggca tctcgacgga
     8281 acagggagag cagtgcgagc agacagaggc cgatcagtaa cgccaccagg tactgccggg
     8341 cgacgagcgc tatcaggatg acggccatga gcgcagctac catccacggc agaagttcca
     8401 cccagggact cggtcgtacc aggactctcc acaactcctc gcgccgatcg tagcgcgcca
     8461 atcctgtgaa ccgctcgtaa gcctcgctcc attggaaccg cagccaccgg tccttgacca
     8521 cagcgtatat gagggcggtg gagatgcaga agaggaaaag cccgaacatg acgaggtagt
     8581 agcgaaagtc cgtcttcgcc ggagcagggt cgactccgtt gtagaagagc tcatagttgc
     8641 gcgtgtacgg ggagaaaaga atcattgcga cgacgtacgt gaagccggca gatccggcag
     8701 cccagatgag gccatcgatc gtgatcgccc ggtcgcgggc atagctggcg accaggaagg
     8761 acgcagaaat cagtatgaga taggtaggga agtcccaact gttggtcgcc tgcagcatcc
     8821 cgaccacgaa ccccgcgaac aggaccgaga gccctcgctc cgcaagggac cacccgcggg
     8881 gcgcctcggg aatcgtcgcc tcctccccgc cagcgattgg caggtcccgt ccagcgagac
     8941 tatctccggt gacagcacca tcccgctgcc ctaccattcc ggccacgacg atctttgttt
     9001 ggggcaaccc tgccagtcgt tccgcattct ccttgtccgc agggcgcctg cttccggcct
     9061 gccacacgat ggcgagagtc agtccgagag ccagcacagt cagcggcatg gcgatcatgt
     9121 gggcgtgtag gtcgccgtac aggaaagtga accatgggaa ctccataatc ggagttgggt
     9181 tctcccccgg tatgagtctc gtcgaacgcc agaagtccag cggaggaaga gtcttgcccc
     9241 cgagcaggac cgcggacaac ccgtatcccg ccttcaccac accttccaac cccgggattg
     9301 tacttctgaa ctgggaaccg ccgagcgccc acagagactc gagccgctgg atcagaccgt
     9361 cgacgttgcc tgcaaccagg acaaatacgg tggcggcaag gccggtggca acattaccga
     9421 gagacagcag tctcgcacct atctgcctta cggcagcgaa gttgagcgcg aacgagaaga
     9481 ggcccaccgc ggtcagccca tagatcgtcg gaatggcaag gttgaaagct accgcgggga
     9541 caatccccgt gagacgcatc aaagtggcaa ctattacgta gccgaagtag tagtagttga
     9601 tgtaaccacc tgcaaaccag gggtcgtagg gcgggaagta ggtagtcttg gcgatggcat
     9661 tcaggtaggc aaactccatc ggcttctcgc cgccaaagct gggatgccag aggtcagggt
     9721 tgctcatccg gatggccaga aacagtccga atgccgcgag gaagaccaac tcctccgcga
     9781 gtatcagcct cccgccctgt cgggcgaaac cccagaactc tctcgcgcga cctcggagcg
     9841 cgaccgcgcc aattccagcg agcagcgccg tgccagcgag ggcagtcatc ggcccgaacg
     9901 cggcaaggcg aaagcttgga ataatccagg caacataggc tgtcaccgcg atgccgatcg
     9961 ccttcgcgaa accatagccc cggtcgggga accggcggaa cagcaaccat gtgatcggaa
    10021 gcgccagcag cccgaggacc tcgaccaata gaagccaggc aagaaccgga acacggttgg
    10081 ccaggctgtt cacgtcgtaa atctcggacc acgtgccccc tgcctgcgcc gcatctctgt
    10141 cggcgctaga cagcagcagc ccaccgtact gcgcctcgac tgacttctgc tgtacaacgg
    10201 agtccagcgg aaccgctccc aggagttgcc gcgtcttcgc tggcgagtag tcggccgtct
    10261 tgtggaaaat gagcaccttg gggtggtcgt agacgctgaa gatctcctcg gcgctatcgt
    10321 cgttgatctc gatcccgaag atcgttggat gcgaagtgaa ggtcttctcc aagcggaacc
    10381 caagctgtcc cgagaagagc agcctgtagt actcggtggt catcgggtac ttgagcggca
    10441 tgcgggggat cgagccgtaa agccggttgc tgcttatgaa gatatattcc gtcgtgtcca
    10501 actgctgcac cagcttgttg tacttctcgg gcgtgtcgtc gttgtacagt tctagcatag
    10561 tgccgctgta ccaaccggca tcgcgccctt tccagcgcag tggaagagga tcgtcccagt
    10621 gctcgttggc gatggtcgtg ccctgcggga tgttcgcgaa gatccactcg gtggcttcga
    10681 tccgagtaac cggcctcgtg tatatctggg taaaggcata tgcatacgcg agggtgcaga
    10741 cggcgaccgc gagagtgacc gcaggcagtg catatgtcgc cgcccgcgca agagtggcac
    10801 gccggtcaga ggtccacacg cgctccctgg ctctgtcgaa cccagcgacc agcatctgag
    10861 caccgaggag cgacagaagg gggacgatgg gcaggaaata gcgcatagtt ttcgcgaatt
    10921 gcccgccctg atataggaag aagaatccga cccaggcaac cagcagcagg tacttagttt
    10981 cgcgccggaa gagcagcagg aacgcaccca tgacgaggcc aacccagcca acaacaccga
    11041 ggggaagccc catgccccag aacacgaggt tgcgcaaggg gaatatgtag gcaggcgtgt
    11101 tggcccattg cacgctggga ggctggtcga cttctccact gaccaacttc tggacaaagc
    11161 cgatatcgtt caggaacttg gtcgagaggc caatgttaaa gaatcccggc cccgcgaaag
    11221 catagggttc gaccacgcgg aataccagta gagcaacgag tgctactccc gcgaaacgcc
    11281 cgatgaccgc catccagtcc gccatccgct ggttcgggaa taccagggtc acctcccgga
    11341 gttgcttcac ggctattaag gccacgacaa tcgcgaagac agcggagttg atcttgcacg
    11401 ccatcgcgaa gccaagagag gcgccgagca gcggatagtc gtatgcatgc ggcgactccc
    11461 agatcctgac ggcgaagaag aacgagacga gcaggaagaa cccgacgaag gtgtcaaaag
    11521 tgaagaagtg cgattgctgg atctggatga ctgccccagc ggataggagc gaggcgagca
    11581 atccggacct ggacccgaaa agccggcgcc cgatgaagaa gatcatcaga accgacccaa
    11641 tgtcgaacaa agcggagacg accctcccga cgaggtgtat ctcgtcgtag ccgctcatgt
    11701 tgagcacaat accgagccac ttgaccagga agagcgggaa cgtaccatac acgaagctgt
    11761 tgtagccgcg gttatagggg ttcagcggcg atcgcgcggt gtcaaagtat tggccgaggc
    11821 tagtgggcca gtcgagcgag ttctccacca tcgtgatgaa tcgctcgtcc gggtgcagat
    11881 gcgtgaagct atcccagttg acgcccgtca ggcggaagaa ggcagcgacc gcaagtatcg
    11941 ctgtaaggca gaggagcgtg gcaacggcag ccaggggtcc gcgaccttgg cggagcgtgg
    12001 aatcagacac ctgcctcctc cactccaacc gatgcgggct gagacttcct gcacaccagg
    12061 aagatgtatg ccccttctcc ttctggatcc tgctcgtaga agcgggagaa gaggctgcgc
    12121 cggatggcgg tcggcttgtt tggaaacagg atccaccggc cggtcaaccg tcttacgatc
    12181 acggaaggtg cgctcaggta gtggctcaga tcaaaggccc tgt
//
LOCUS       DATALS010000014         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_181470, whole genome shotgun sequence.
ACCESSION   DATALS010000014 DATALS010000000
VERSION     DATALS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181470"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VHS06_00325"
     CDS             <1..670
                     /locus_tag="VHS06_00325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX2986612.1"
                     /translation="LTQFLGCPDRGDHTELPSLEKDEDEVEGALGQISLEGQHMLVGI
                     HPGAKAATRSWSPERFGRVARRLHKEYGALITVTGTDRERTIVEAVTEACGPGAVNLA
                     GRLSVGGLAALIRRQALLITNDTGPAHLAYATQTRSVTIFGSAEPERWRGVDTTRNTA
                     LSVSVPCRPCEYSECPIGYLCLNMVTVDAVARAARFLLDDWVRSRLPAIWPNTDLSTD
                     AIAG"
     gene            786..1997
                     /gene="amrB"
                     /locus_tag="VHS06_00330"
     CDS             786..1997
                     /gene="amrB"
                     /locus_tag="VHS06_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX2986613.1"
                     /translation="MQPKLRSALEPQWIQHEGRDALLVRDKLGISDQAIVLPEPLAVL
                     VSLFDGQRDTAAISAAFQLRTGMRVSRAYVDALVQQLDDVLFLDSPRYEAAYRKVLDE
                     YRNQDSRKPSLAGAGYPAAVAQLDSLLLGYLDGAEGPSVDGRFIRGIISPHIDFARGG
                     RTYAKVWKRAEASLREAEIIIVFGTDHLGGPGEITLTRQSYATPYGVLPTAVEVVDAM
                     AQAIGHEEAFASELHHRSEHSIELAAVWLHHSIDSRRCDIVPVLCGSFQPFTDGDRDA
                     ARDERLNAALDALMAATRGRRTIAVAAADLAHVGPAFGDPVPLGPLEKATLASDDSQM
                     MERVLTGDAGAFLQRLQEEGDRRRICGLPPIYMMLRFLEDSRGEMIGYEQCPADANGG
                     SIVSITGALFY"
     gene            complement(2159..3250)
                     /locus_tag="VHS06_00335"
     CDS             complement(2159..3250)
                     /locus_tag="VHS06_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2986614.1"
                     /translation="MKAWRVYGLGDMRLDDIPCPDLRPGWVIVKTRVVQPSVTEAQRA
                     LGIPTGGSDYIRKIIAERAPVQLFGHEFCAEVVEVGEGVMGLQPGDRVAARSEVPCGQ
                     CELCRTGYGDRCRKGPIIGRQIPGCLAEFAAMPSEALVKLPDSVSDSEGAWIQPLSSA
                     VAAVASARLEMGDSVLVLGQGAMGICALQVARVAGAGLVIGADVRAQALEMARSLGAD
                     VTVNAGEDLADAEMLEATGGLGADVVIEAAGGNPGQGLAGDRALRRAFQLVKDGGRIV
                     QIAYLGAPIEVAVDSLIRARGLKYLSADVPSVRLLEYAVRLVASGRVQVKPLITHVLK
                     GLESVPAAFEITANKAKYGAAGPAQVHLC"
BASE COUNT          742 a         1174 c         1098 g          645 t
ORIGIN      
        1 gctgactcag ttcctaggat gtccggacag gggagaccac acggaactgc cgagtctaga
       61 gaaagacgag gatgaggtcg agggtgcgct cggacaaatt agcttggaag gccagcacat
      121 gcttgtgggc atccaccccg gggcaaaagc cgcgactcga agctggagtc cggaacggtt
      181 tgggcgggtg gcgaggcgcc tgcataagga gtacggagcc ctgatcacgg ttacagggac
      241 ggatagggaa aggacgatcg tcgaggcggt gactgaagcc tgtggccccg gtgccgtcaa
      301 cctggctggg aggctgtcgg ttggggggct ggccgccttg ataaggcggc aggcgctcct
      361 catcacgaac gacaccgggc ctgcacacct cgcctacgca acccaaacca ggagcgtcac
      421 aatattcgga tccgccgagc cggagagatg gcgaggcgtc gatacgacac ggaacaccgc
      481 gctctccgtg agcgttccct gcaggccgtg cgagtactcg gagtgtccga taggctacct
      541 gtgtctgaat atggtgacag tagatgccgt cgcccgagcc gcgaggtttc tactcgacga
      601 ttgggtccgc tccaggctcc cggcgatatg gcccaacacg gaccttagca ccgatgcaat
      661 tgccggatag gctgaccggc actagctcag aatacttgcg cccacggcgc gtcttcgagt
      721 agtatatcgg ttagcgcgct ggcaccatgg tccgccccgc gcagttcagc gaccggagag
      781 agccgatgca accgaaactg aggtccgccc tggaaccgca gtggattcag catgagggtc
      841 gggacgcact tctggtgcgc gataagctgg ggatatcgga ccaggcaatc gtcttgccgg
      901 aaccccttgc cgtcctcgta tcgctatttg acggccagcg agacaccgcg gccatcagcg
      961 cggcattcca gcttcgcacg ggcatgcggg tgagccgggc ctatgtggac gcgctcgtgc
     1021 agcaactcga cgatgtgctc tttctggata gccctcgcta tgaggcggcc tacaggaagg
     1081 tgcttgacga gtacaggaat caagactcaa gaaagccatc gcttgccgga gcagggtatc
     1141 ctgcagcagt ggcccagctc gattccctgc tgctcggcta cctggacgga gcggaaggcc
     1201 cgtctgtgga cggaagattc atccgcggta tcatcagccc ccatatagac ttcgcgcgtg
     1261 gcgggcggac gtacgccaaa gtgtggaaga gggcggaggc ctcgctgcga gaagctgaga
     1321 tcatcatcgt cttcgggacc gatcacctgg gggggccggg tgagatcacc ctgaccaggc
     1381 aaagctatgc caccccttac ggagtgctcc cgacggccgt ggaagtcgtc gacgccatgg
     1441 ctcaagcgat cggccacgaa gaggctttcg caagcgagct ccaccatcga agcgagcact
     1501 ctatcgagct ggcagcggtt tggctgcacc actcgattga cagccgcaga tgtgatatcg
     1561 tccccgtcct gtgtgggtcg tttcagcctt tcacggatgg tgacagagac gcggcacggg
     1621 acgaacgtct caacgccgct ctcgacgcct tgatggctgc tacgcgcggg aggcgaacta
     1681 tcgccgtggc tgccgccgac ctggcccacg ttggtccagc gttcggagac cctgttccgc
     1741 tcggtcccct ggagaaggcc actctggcga gcgacgactc gcagatgatg gagcgtgtgc
     1801 tgacgggaga cgctggggcc ttcctgcaaa ggctgcaaga ggagggcgac cggcgcagga
     1861 tatgcgggct gccgccaata tacatgatgc tcagattcct cgaggactcc aggggcgaga
     1921 tgatcggtta cgagcaatgc cccgcggatg ccaacggggg atcgatagtc tccataaccg
     1981 gcgccctgtt ctactagttc aacccgccga gaactgttgc cactggctaa gccctaccac
     2041 ggggcaagaa gaaagacgag ggctttcggg ccagacccac gcaaggagcc gacagagtag
     2101 ctcacgggct tcctctctaa ccttgaccag atcactcgca gtgcgggaga gcgccttcct
     2161 agcacaggtg aacttgtgca ggtcccgccg ctccatactt ggccttgttg gctgtgatct
     2221 cgaaggcagc cggtacgctt tccagaccct tgagaacgtg ggtgatcagt ggcttcacct
     2281 gcacacgacc ggaagcaaca agccttaccg cgtactcgag cagccggacg ctaggcacat
     2341 ctgccgagag gtatttgagt cctctcgctc tgatgagcga gtctaccgcc acctcgatgg
     2401 gtgcccccaa gtaagctatc tggacgatcc gccccccgtc cttcaccaac tggaaggccc
     2461 gacgcaacgc ccggtcgccc gcgagtccct gaccggggtt ccctccggcc gcttcaataa
     2521 cgacgtcggc acccagcccg ccagtcgcct caagcatctc cgcgtccgcc agatcctcgc
     2581 ctgcgttcac cgttacatcg gcccccagtg atctggccat ctcaagggcc tgcgcccgca
     2641 cgtcggcacc gataacaagg cccgcaccag ctacacgggc aacctgcagt gcacagatcc
     2701 ccatcgcccc ttgtcccagg accagaacgg agtcgcccat ctcgagccgc gcgctcgcta
     2761 ctgccgcaac tgcgctgctc aacggctgaa tccacgcccc ttcgctatca ctcactgagt
     2821 ctggaagctt gacgagcgcc tccgagggca tcgcggcaaa ctcggccagg caccccggga
     2881 tctgccttcc aatgatcggg cccttcctgc agcggtcgcc atatcctgtg cggcacagtt
     2941 cgcactggcc gcatggtacc tcggaccgcg ctgccacgcg atctcctggc tggaggccca
     3001 tcactccctc accgacctcc accacctccg cgcagaactc gtggccgaac aactgcaccg
     3061 gtgcccgctc tgctatgatc ttcctgatgt agtcagagcc tccagtggga atgccgaggg
     3121 cccgctgcgc ttcagtaacg ctcggctgca ctacacgagt cttgactata acccatcccg
     3181 gccttagatc cgggcagggg atatcgtcca gacgcatatc cccaaggccg tacacacgcc
     3241 aagccttcat gtaacctccc cctagcttcg aaatcctaca ctcagcgacc cggcccagag
     3301 tgacgcggcc gccttgacca ggagccagac tgcctgactc ctggcatgga tcctgggaac
     3361 catgccggcc cactatgccg tgacgggcta caaacgacgc cttgcaggct gcgaattagg
     3421 gttgatgcga agagaacaaa taactgttga cacacagccg aagttcgaca gggaacgtgc
     3481 caatcactac gaagcgtatc atagcatgcg tgctcagcag tgtcaagcat ccggcatgcg
     3541 cggcaagtgc cggcgacgaa gaacaactgg gagctgtccg ggtcatacgg gttccctctt
     3601 gctactacgc tcgcgtgaac tccgttgcgg acgaaggccc cgaagatacc gagctctac
//
LOCUS       DATALS010000015         3551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_136465, whole genome shotgun sequence.
ACCESSION   DATALS010000015 DATALS010000000
VERSION     DATALS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136465"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3420)
                     /locus_tag="VHS06_00340"
     CDS             complement(<1..3420)
                     /locus_tag="VHS06_00340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX2986615.1"
                     /translation="MSSDQASPSTGNQEFVHLHVHSEYSLLDGLGKVKNLVNRAKELG
                     MPALALTDHGSMYGAIEFYLAAKAAGIKPIVGVETYMARRRMTDKEAEDKSSSHLLLL
                     AKDLSGYQSLVQLVSAAHLEGFYYKPRIDRELLEKHHEGLIICSACASGEVARAILRD
                     DMDGAMKTAAYYRDLVGPENYYMELQDHGLVDQKKINVGVLELNRRMGIPVIATNDVH
                     YVLKEQAVAQEILLCIQTNSTLDDPNRMRLETQNFYLRTAEEMAELFREVPESITNTL
                     RVAEQCDLHLDSDRVILPDLAFIPKDMPPIEYLAKLARKGLEERYPNPSEEIRNRLEY
                     ELSVIEKTGFPKYILLVYDYVNWARKQGIFCQPRGSAAGSIVLYCLYIADVDPMEYVL
                     TFERFLNPDRASMPDIDMDFQDDRRQEVIDYVTRTYGADCVAQIVTFGRLAARAAIRD
                     VGRAMDIPLSEVDKVAKLVPTLPVGMTIDKAMEENPDLKAMYENEPHIKRLIDAAKSI
                     ESVARNTSTHAAGVVVSRDPLTQVVPIQKATKGEELVMCQYPAKMLEKMGLLKMDFLG
                     LNNLTIVANSLKLIKETCGEDLDLAKIPLDDAGAFKMLADGETTGIFQLEGTGMRRWV
                     KELKPSTVRHLMAIIALYRPGPMACIPTYIARKEGREKIEYFHPKLEPILRETYGVIV
                     YQDQVIKGVVAVAGFSMGQADILRGAMSKKIRDVMAKQKDLFLIGAQKNGITEAGANQ
                     IWEQIEPFAGYAFNKAHAACYALLAYQTAYLKTHYPVEYMTALCTSYVSDTDKIALTL
                     GECRRLGIKVLPPHINHSQLQFSVEPQSANGSTQKPVDCSTRRLAMRFGLIGVKNVGE
                     GAIISMIEEREKNGPYISLSDFCKRVDSKTINKRVIESLIKCGAMDEFGDRAVLLEGL
                     DQTLDAAQQEQKAKEIGQITMFDSMFGGAAAPTASAAPARSTNAVPTTTLKQLLLWEK
                     EILGLYISSHPFQHAAPFLQNRVTANSSQITEEMEGKKVVIAGVVSSVKVLTTKKGDR
                     MLAAQMEDLLGGLEVVVFPRTYERTKSIWEEDAVVVVEGKVDAKDERKKVLAEKAERF
                     VVPEGVEPPPMMEMGSEAMGSEDVATGLDSEPEPPAFDDEPDFGGWDDLDGE"
BASE COUNT          572 a         1080 c         1136 g          763 t
ORIGIN      
        1 ttcgccgtcg agatcgtccc aaccaccgaa gtcgggttcg tcgtcgaagg cggggggctc
       61 cggctcagaa tcgaggccgg tcgcgacatc ttccgacccc atcgcctcgg atcccatctc
      121 catcatgggg ggcggctcca ccccctccgg caccacgaac cgctccgcct tctcggcaag
      181 cacctttttc cgctcgtcct tggcgtccac cttcccctcg accaccacca ccgcatcctc
      241 ttcccagatg gacttggtgc gctcgtaggt tctggggaat accaccacct ccaacccgcc
      301 gagaaggtcc tccatctggg ccgccagcat tcggtcgccc ttcttggtag tgagtacctt
      361 gaccgaggag acaaccccgg ctatcaccac ctttttgcct tccatctcct cggtgatctg
      421 gctgctgttg gcggtgaccc gattctgcaa gaagggcgcg gcgtgctgga atgggtggct
      481 ggagatgtac agccccagga tctccttctc ccacaacagc agctgcttca gcgtggttgt
      541 ggggactgca tttgtgctgc gagccggggc tgccgaggcg gtgggggcag cggcgccgcc
      601 gaacatggag tcgaacatgg tgatctggcc gatctccttc gccttctgct cctgctgggc
      661 cgcgtccagc gtctggtcca gcccctccag cagtacggca cgatccccaa actcgtccat
      721 ggcgccgcac ttgatcagac tctcgatcac ccttttgttg atggttttcg agtctacccg
      781 cttgcagaag tcggagaggg agatgtatgg cccgttcttt tcgcgctcct cgatcatgct
      841 gataatggcg ccctctccga cgttcttcac gccaatcaac ccgaagcgca tggctagccg
      901 cctggtgctg cagtccaccg gcttctgcgt actcccgttc gccgactgcg gctccacgct
      961 gaattgcagt tggctgtgat tgatgtgcgg tggcagcacc ttgatcccca gccttcgaca
     1021 ctcccccagt gtcagggcga tcttgtcggt gtctgacacg taggaggtgc agagggccgt
     1081 catgtactcg acggggtagt gggtcttcag gtaggcggtc tggtaggcca gcagtgcgta
     1141 gcaggcggcg tgggccttgt tgaaggcgta tccggcaaag ggctctatct gctcccagat
     1201 ctggttggcc ccggcctcgg tgatcccgtt cttctgggcg ccgatcagga agaggtcctt
     1261 ctgcttcgcc atgacgtcgc ggatcttctt gctcatggcg ccgcgcagga tatcggcctg
     1321 gcccatggag aagccggcca ccgccaccac gcccttgatg acctggtcct ggtagacgat
     1381 caccccgtag gtctcccgga ggattggctc cagcttgggg tggaagtact cgatcttctc
     1441 tcggccctct ttgcgggcga tgtaggtggg gatgcaggcc atcgggccgg ggcggtagag
     1501 ggcgatgatg gccatcaggt ggcggacggt gctgggcttc agttccttca cccaccgacg
     1561 catccccgtg ccttccagct ggaatatgcc ggtggtctca ccgtcggcca gcatcttgaa
     1621 tgcccccgcg tcgtccagag ggatcttcgc caggtcgaga tcctcgccgc aggtttcctt
     1681 gatgagcttc agggagttgg ccacgatggt gaggttgttc aaccccagga agtccatctt
     1741 cagcaggccc atcttctcca gcatcttcgc ggggtactgg cacatcacca gttcctcacc
     1801 tttggtggcc ttctgtatcg gcaccacctg ggtgagggga tccctggaga cgacgacgcc
     1861 tgcggcgtgg gtcgaggtgt tgcgggccac gctctcgatg ctcttggccg cgtcgatcag
     1921 ccgcttgatg tggggctcgt tctcgtacat cgccttgagg tcggggttct cctccatcgc
     1981 cttgtcgatg gtcatgccca cggggagggt ggggaccagc ttggcgacct tgtcgacctc
     2041 ggagaggggg atgtccatgg cgcggccgac gtctcggatg gcggcgcgcg cggccagccg
     2101 gccgaaggtg acgatctggg cgacgcaatc tgccccgtag gtcctggtga cgtagtcgat
     2161 cacctcttgg cggcggtcgt cctggaagtc catgtcgata tcgggcatcg acgctcggtc
     2221 cgggttcagg aaccgctcga aggtgaggac gtactccatg ggatcgacgt cggcgatgta
     2281 gaggcagtag aggacgatgc tgccggccgc ggagcccctc ggctggcaga agatgccctg
     2341 tttgcgagcc cagtttacgt aatcgtagac cagcaggatg tacttgggga agccggtctt
     2401 ctcgatgacg gagagttcgt actccagcct gttgcggatt tcctcgctgg ggttggggta
     2461 gcgctcctcc aaccctttgc gggccagctt cgccaggtac tcgatggggg gcatgtcctt
     2521 ggggatgaag gccaggtccg gcaggatcac ccggtcggag tccaagtgga ggtcgcactg
     2581 ctccgccacc ctcagggtgt tggtgatcga ctccggcacc tcccggaaga gttcagccat
     2641 ctcctccgcg gtgcgcaggt agaagttctg ggtctccagc cgcatccggt tggggtcgtc
     2701 cagggtggag ttggtctgga tgcagagcag gatctcctgc gctacggcct gctctttcaa
     2761 cacgtagtgg acgtcgttgg tggcgatgac cgggatgccc atccgccgat tcaactccag
     2821 gacgcccacg ttgatcttct tctggtcgac caaaccgtga tcctggagct ccatgtagta
     2881 gttctcgggg cccacaaggt cgcggtagta ggcggcggtc ttcatggcgc cgtccatgtc
     2941 gtcgcggaga atggcgcggg caacctcgcc ggaggcacag gcggagcaga tgatcagtcc
     3001 ctcgtggtgt ttctcgagta gttctcggtc gatgcggggc ttgtaataaa agccttcgag
     3061 atgggcggcg ctcaccagtt ggacgaggga ctgatagccg ctcaaatcct tggcgagcag
     3121 cagcaggtgg ctggaggact tgtcctcggc ctccttatcg gtcatgcggc ggcgggccat
     3181 gtaggtctcg acgccgacta tcggcttgat gcctgcggcc ttggccgcga ggtagaactc
     3241 tatggccccg tacatggagc cgtgatcggt gagggcaagg gcgggcatgc ccagctcctt
     3301 ggcgcggttc accagattct tgaccttccc cagcccgtcc agcagagagt actcagagtg
     3361 gacgtgaagg tggacgaact cttggttgcc ggtgctgggg gatgcctgat cagaactcat
     3421 agacagcgaa cctcgtaaca gatgcggcgt cgcaggacaa agctggtcgg tcccagcccg
     3481 cgagggctga cagcggggct ttagctccgg gcgaaggttg ccatcagcta caacttgcta
     3541 atagcagaaa a
//
LOCUS       DATALS010000016         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_361740, whole genome shotgun sequence.
ACCESSION   DATALS010000016 DATALS010000000
VERSION     DATALS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361740"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VHS06_00345"
     CDS             complement(<1..1380)
                     /locus_tag="VHS06_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2986616.1"
                     /translation="MPPDVWSPWATLGATSPRLDGSPVPRESRAPLAFLPPWSRNADL
                     ERVLAVKRPWAIEDLEAEPLVEPLLPYARATGVRAVLGTALFRGEELVGLLLLHQCDR
                     TRRWTKRELDLIQAVAAQLVVAIENARLYEETDERLRSRVSELGSLVRLGTAVSQQLS
                     MDIVMGRAAEEGARALQADRCAVATMDSDRGVLVSRAVCDYCGEPTMGVGIEMTATQY
                     PGSYRAVASQHPVLMTPGDPSLSPLESRLLVRMGMSTAVVAPMAVGDRAIGTIFFGRR
                     PERPGFSDDDVALAGALAATVAVALQNATLFQEVQAQKSRTGALLASMSEGVCAVDVN
                     MRITDVNPWLEAMVGYRADQLVGSMCGEVMCHIDEEGNRLCSTSCPLRTALREGTGTD
                     PITVFIQTAWGELLPVLVSAAPIRDELMQVVGAVSVWRDVSREWQMDRLRSNIISVVS
                     HEFRTPLTSI"
BASE COUNT          299 a          527 c          419 g          264 t
ORIGIN      
        1 aatgctggta agcggggtcc gaaactcgtg ggatacgacc gagataatgt tggagcgcaa
       61 ccggtccatc tgccattcgc ggctcacatc cctccaaacc gaaaccgccc ccacgacttg
      121 catcaactcg tcccgaatcg gtgcagcact caccagcacc ggtagaagct cgccccacgc
      181 agtctggatg aagacggtga tggggtccgt gcccgttccc tcccgcaaag cggtacgcag
      241 cgggcaagag gtggaacaga ggcgattgcc ctcctcatcg atgtggcaca tcacctcccc
      301 acacatgctg cccaccagct gatccgcacg gtagccgacc atggcttcca gccaagggtt
      361 gacatcggtg attctcatgt tcacgtcgac ggcacacaca ccctcactca tggaggccag
      421 cagcgccccc gtccgggact tctgagcctg aacctcctga aaaagagtgg cgttctgtag
      481 agctacggcc accgtcgcgg caagagcgcc ggccagggcc acgtcgtcat cggagaagcc
      541 ggggcgctcc ggccgacggc cgaagaagat ggtcccaatg gcacggtccc ccacggccat
      601 tggggcaact actgccgtcg acattcccat ccgcaccagc agccgcgact ccagtgggga
      661 gagcgatgga tcgccgggcg tcatcaacac agggtgctgg ctcgccacag ctcggtagga
      721 tcctggatac tgggtggccg tcatctcgat gcccacaccc attgtcggct cgccacaata
      781 gtcgcagact gccctcgaca ccaggacccc cctatccgaa tccatggtgg ctaccgcgca
      841 acggtcggcc tgaagtgccc gcgccccctc ttcggcggca cgtcccatga ctatgtccat
      901 gcttagctgt tggctcaccg ccgtgccgag acgaaccagg ctacccaact cgctcacccg
      961 tgacctcagt cgctcgtccg tctcctcgta cagcctggca ttctcgattg ccactaccag
     1021 ttgagcggcc acggcctgga tcaggtccaa ctccctcttg gtccaacgtc gagtacgatc
     1081 acactggtgc agcagcaata gcccgaccag ctcttcgcca cggaacagcg ccgtgccgag
     1141 cactgccctg acacccgttg ctcgagcata gggaagcaac ggttcaacga gcggttcggc
     1201 ttccaagtcc tctattgccc agggacgctt caccgcgagc acacgttcga ggtcggcgtt
     1261 ccggctccag gggggcagaa acgctaaagg agctctcgac tccctcggca caggactccc
     1321 atctagcctc ggcgacgtcg ctccgagagt tgcccagggc gaccacacat cgggaggcat
     1381 ctatggcatc cctcagctcc ctgggttgcc gtcgataacg ctcagttgat gcaagaggcc
     1441 agggagagcg ctgcccacga agcgttgatc catcgcatcg cttcatccct cggttcttcg
     1501 ctggacctg
//
LOCUS       DATALS010000017         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_316182, whole genome shotgun sequence.
ACCESSION   DATALS010000017 DATALS010000000
VERSION     DATALS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316182"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1272)
                     /locus_tag="VHS06_00350"
     CDS             complement(136..1272)
                     /locus_tag="VHS06_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986617.1"
                     /translation="MPEMTRRERLLEATHGRPADKLPFFHYWRHSQIGWAERECRNRG
                     MGMNWDRPCFTSRLHDVRVVEERAVVDGQPVLRRTYHTPLGSVYEDEIREPGVGQWHA
                     NRSWKDVTPWLSHRMINGPADYPIVKFIVEHTEYLPDYFPIEQAMDWLGEEGLVIDHL
                     MHSPMQMLMIHWVGSDGGKFYFHLADDPDLVEDLYRATVKSRLPLYEIAAGSPAPITL
                     CGDNVDGFLVTPKLFEKYFMPVYAQQAEALHRRGKLMAVHMDGRLSNLRHLIARTPID
                     IVEALHPRPMGDLPVGEALSAWPGKAVWLGFPGGVYESGPQATREYAVDLLRELGSGE
                     RLAVCMSTENLVSNQNLLAVTDVLEKASLPLSPSKIEDIRRRLL"
     gene            complement(1345..1554)
                     /locus_tag="VHS06_00355"
     CDS             complement(1345..1554)
                     /locus_tag="VHS06_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986618.1"
                     /translation="MSPVPDKTLAELKGQEVTVSFSTPVHKLRSLTGPLQDFDPAFIT
                     IGGDGGRKIERQAIISVRPKQAGRR"
     gene            complement(1704..2279)
                     /locus_tag="VHS06_00360"
     CDS             complement(1704..2279)
                     /locus_tag="VHS06_00360"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEX2986619.1"
                     /translation="MPPANQPEYPFPTLPDYLAPGLGLVLVGINPSIYAVQQGHYFAR
                     PSNRFWPALSRSRLSQSIRLALGRDRLAPADDGALLQFGIGFTDIVKVPSNSIAHLRP
                     ADYREWAPRLLRRLETYAPRLACFHGITGYRAFVQYGLGEVQSDWSLGLQSRRLGPTR
                     VFVVPNPSGANAHFTLADQVAWYDSLATYLQ"
     gene            complement(2266..>2460)
                     /locus_tag="VHS06_00365"
     CDS             complement(2266..>2460)
                     /locus_tag="VHS06_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986620.1"
                     /translation="LKGISDIGPAACLHKPFELTTLVNLVDQHSDGAAFNGGGPGRPP
                     GRNSHDPLRDRFEGQSAAAS"
BASE COUNT          434 a          741 c          816 g          469 t
ORIGIN      
        1 ctggccaagg gccacctccg ggcgaggtga tatgccgggc cccgggaagg gctccggccg
       61 gccttcggtg cgagctcctg cccacccagg ccagattcga agcaattcta gccggggcgg
      121 gcagcactag tcaattcaga gcaggcggcg tctgatgtcc tctatcttgc tagggctcag
      181 cggcagggat gccttctcca agacgtccgt cacggccaga agattctggt tggataccag
      241 gttttcggta gacatacaga cggccagtcg ctctcccgat cccaactccc gcagcaggtc
      301 aactgcgtac tcccgggtag cttgcggccc cgactcgtag actcctccag ggaagccgag
      361 ccacacggcc ttgccgggcc aggcggagag agcctcgcct accgggaggt ctcccatggg
      421 cctgggatgc agcgcctcta cgatgtcgat cggagtgcgg gcgattaggt gcctcagatt
      481 ggacagccgg ccatccatat gcacagccat cagcttgccc cgccggtgta gggcctcggc
      541 ctgctgggcg tacactggca tgaaatactt ctcaaatagc tttggagtga ccaggaagcc
      601 atcgacgttg tcgccgcaga gggtgatggg agctggcgaa ccggcggcga tctcgtataa
      661 cgggagacgg ctcttcacag tagcccgata aaggtcctcc accaggtccg gatcatcggc
      721 cagatggaag tagaacttgc ctccgtcgct gcccacccaa tggatcatca gcatctgcat
      781 gggggagtgc atcaggtgat cgatgactag gccttcttcc cccagccagt ccatagcctg
      841 ctcgatgggg aagtagtcgg gcaggtactc ggtgtgctcc acgatgaact tgacgatcgg
      901 gtagtctgcg ggcccgttga tcatccggtg agatagccat ggggtgacat ccttccagct
      961 acggttggca tgccattggc ccacgcctgg ctcccggatc tcgtcctcgt agacgcttcc
     1021 caggggagtg tggtaggtgc gccgcaatac tggctgccca tccaccactg ctcgctcctc
     1081 caccacccgg acgtcgtgca gtctggaggt gaagcaaggc cggtcccagt tcatgcccat
     1141 accgcggttg cggcactcgc gctctgccca gcctatctga ctgtggcgcc agtaatggaa
     1201 gaacggcagc ttgtctgccg gccggccgtg agtggcctcc agaagtctct cccttctggt
     1261 catctcaggc atgatagtct ccctccgcaa ttacgcgcgc cggtctcgcg atgtgacagc
     1321 ctggcgttgg caagctcggg agtcttagcg gcggccagcc tgtttggggc ggacgctgat
     1381 tatcgcttgc cgttctatct tgcggccgcc atctccgccg atggtgataa aggcagggtc
     1441 aaagtcctgc agcgggccgg tcaaagaacg gagcttgtgg acgggggtgc tgaaggagac
     1501 ggtgacttcc tgccccttca gttccgccag agtcttatcg ggtactgggc tcactcgatc
     1561 gctcctctcg ccggtcccat ctgctgtggc tggcccggct ttgatcccga tgtgccgatg
     1621 gactgctccg gtattataca tcggttgggg agccagacag aaggaaggcg ctgcgtgtcc
     1681 ccggccttgg ccgaggcagc cagctactgc agataggtgg caaggctgtc gtaccaggcc
     1741 acctggtcgg cgagcgtgaa atgagcgttg gccccgctag gattgggtac cacgaacacc
     1801 cgagtggggc cgagccgccg gctctgcagg ccgaggctcc aatcgctctg aacctcgccc
     1861 agcccgtact ggacgaaagc ccggtagccg gtgatgccgt ggaagcacgc gaggcggggc
     1921 gcgtatgtct ccaagcgccg caggaggcgc ggtgcccact cccggtagtc ggctggtcgt
     1981 agatgggcga tgctgttgct gggaaccttc acgatgtcgg tgaagccgat accgaactgc
     2041 aacagggcgc cgtcatcggc cggtgccagg cgatcgcgtc ccagcgccaa gcggatggac
     2101 tggctgaggc gggagcggga aagagccggc cagaagcggt tgctggggcg ggcgaagtag
     2161 tgcccttgct gcacggcgta gatggatggg ttgatgccca caaggaccag acccaacccg
     2221 ggagcgaggt agtcgggcag agttgggaag gggtattcgg gctggttagc tggcggcagc
     2281 ggattgccct tcgaagcggt ccctgagagg gtcgtgtgaa ttccgtccag gcggcctccc
     2341 gggtccacca ccgttgaagg cagccccgtc ggagtgttga tcgaccaggt ttaccagggt
     2401 ggtgagctcg aagggcttgt gcaggcaggc cgccgggcct atgtctgata tgcccttcag
//
LOCUS       DATALS010000018         5447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_136612, whole genome shotgun sequence.
ACCESSION   DATALS010000018 DATALS010000000
VERSION     DATALS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136612"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /gene="priA"
                     /locus_tag="VHS06_00370"
     CDS             <1..1517
                     /gene="priA"
                     /locus_tag="VHS06_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX2986621.1"
                     /translation="QVYMRSIEALLRLGRRAIVLVSEIAQTPEALERYSEQFPGRVAL
                     LHSELPDAERWQLWRGIYDGRFDVVVGPRSALFSPVPDLGLIVLDEEHEPAYKQEDKA
                     PRYHARDVAIELGKRVGATVILGSATPDVGSFYLAERGEYRLAELPDRYSGPSAGGWS
                     TGKLPSVQVVDLREELKTGNTSLLSRPLQRGLERILSLGEQAILFLNRRGQSTCVLCR
                     DCGQVIKCRRCDVPMVYHRDPNELMCHSCNRRAPVPTRCPNCKGRRISFFGAGTERVE
                     EEVKRLFPAARVLRWDQDVTGRQGDHARLHREFREHEADVLVGTQMVAKALDFPLVTL
                     VGVVLADITLHLPDFRAAERTFQLLAQVSGRAGRGSREGEVILQTYSPDHYSVVAASR
                     HDYRAFYEREITFRRQNSYPPFRELARLTYTGTGETRARFEALEMRQHLRSKIEEMGI
                     ADIEILGPAPAFHARVRGKFRWQIVLAGQRLGRLLCEIDLPLGWGLDVDPMSLL"
     gene            complement(1559..2563)
                     /locus_tag="VHS06_00375"
     CDS             complement(1559..2563)
                     /locus_tag="VHS06_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX2986622.1"
                     /translation="MRPFEHLNVTSTDDLLTALGEDWQTRLIAGGTDLLPLMKRRIIA
                     PSRLVNVKTISELTRLDYSDEGGLVIGPLATLTEVERLPAVRQRFRILSEAISVAASP
                     QLRNMATMGGNLCQTSRCWYFRGQFLCWLKGGEKCYAADGENQLHALFGGSPCRSAYP
                     SDPPQALIALGAQMTLCNRQSSCRTMPVEQFFKLPEEGRRRETIIRPDEAITEIRVPV
                     PPDGSRGAYLKAMSRETWAFPLVSVAVQGTWDGTMPREINIVMGGVAPIPWRATQAED
                     LVRGKPLDGNLAREAGEAALADAKPLKRNGYKLELIRTLVQRGLLIASSQKGKYNPTA
                     "
     gene            complement(2560..4869)
                     /locus_tag="VHS06_00380"
     CDS             complement(2560..4869)
                     /locus_tag="VHS06_00380"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX2986623.1"
                     /translation="MGKVVKSEVEYEGNIGEHLTIIEGPEPEPWSQEAKLQTVGKSVD
                     RVDAYEKATGRAQYTSDIHPSGMVYGKILRSPYPHARIVSIDTSRAELLPGVRGVLSP
                     INAPSIRWKDGKLVLDTVLNFVGDEIAVVAADDEETAQDAVDLIRVQYEELPFVLDPE
                     QAMQPDAPRVHPSGNLADGKPRVYERGDMKAGLSQAEVTVEETFRTQVALHNSMETHG
                     TVANWVGEDLTVWDSTQGVFVARAEIAAILGLPLNRVRVISHYIGGGFGSKLEINKQT
                     ILAALLARQIQRPVRIVLSRHEENLITGHRHPTAQTLKLGARQDGSLTAIHLHCVVPV
                     GAYGYAASIAGPAMQLYDCPNVHTEVYAVYTNDGPARAFRAPGYTEGTFALESAMDML
                     ADRLGMDPLELRLKNYADKDPLTGHDYSAKHLREVYQLAAEKAGWAKRHPAGTTQAGQ
                     GAPFTTKKLGIGMATQIWGGGGGPPAYAIVKLNPDGTADVVTGSQDIGSGTATGLAQI
                     AAEALGFTIENVHVSEGDTRDAPYSPTSGGSQTISSTGPAVRAAAEDAREELLEVASQ
                     VMEISRDQLQVKDNRIYVAGSSENGRPVSEVLGSLGNAMIVGRGSRGPNPPGYAIRTF
                     GAQFAEVEVDEETGRIRLLQIVAVHDFGRVINPKLAENQIQGGITQGIGYSVMEERVI
                     DARNGRVLNPDLEEYLIPTSMDTSSIEVAVIGDPDQHSNNLGAKGIGEPPIIPTAPAI
                     ANAVFNATGTRLLNLPMSVRTLLKKRSTP"
     gene            complement(4872..5399)
                     /locus_tag="VHS06_00385"
     CDS             complement(4872..5399)
                     /locus_tag="VHS06_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2986624.1"
                     /translation="MTELAGKRPSWLVPRGPGKTTISLIVNGAEYNIAVQTRRTLLEV
                     LRSTLSLTGTKNGCGMGQCGACTVLLDGVPVYACLILAIECEGHRIETIEGLEKDGKL
                     HPIQEAFIEEDAFQCGYCTSGQIMAIKGLLDNRLEPTLDEVKRAVSGNLCRCGAYPNI
                     FRATLRAAERMGRRR"
BASE COUNT         1006 a         1563 c         1600 g         1278 t
ORIGIN      
        1 cgcaggtcta catgcgatcc atcgaggcac ttctgcggct gggtcgccgc gcgatcgtac
       61 tcgtgagcga gatcgcacag actcccgaag cgctcgagcg gtactccgag cagtttccgg
      121 gaagagtggc gcttcttcat agtgaattgc cggacgctga gcgatggcag ctatggcgag
      181 ggatctacga cggacgtttt gatgttgtag tggggcccag gtcggcgctg ttctctcctg
      241 tcccggatct cggactgata gtcctggacg aggagcatga gccggcttac aagcaggagg
      301 acaaggcgcc gcgctaccat gcccgtgatg ttgcgataga gcttggcaag agggtaggcg
      361 cgactgtgat cctgggaagt gcgacgccgg atgttggctc cttctacctt gctgagcgcg
      421 gcgaatatcg tctcgcggag ttgcctgatc gctattcggg acccagcgcg ggcggatggt
      481 cgactggaaa actcccatcc gtccaggttg tcgatctcag agaagagttg aagacgggca
      541 atactagtct attgagtcgc ccattgcagc gagggttgga aagaatcctt tctttgggtg
      601 agcaggcgat cctgtttctc aataggcgcg gacagtctac ctgcgtgcta tgtcgagact
      661 gcggccaggt gatcaaatgc cgacgttgcg acgttccgat ggtctaccac agagatccca
      721 acgaactgat gtgccatagc tgcaaccgcc gtgctccagt ccccacccgc tgtccgaact
      781 gcaaggggcg gcggattagc ttcttcggag ccggcacgga gcgggtggag gaagaggtca
      841 agaggttatt ccccgcagca agagtgctcc gatgggacca ggatgtaacc ggcagacagg
      901 gcgaccatgc gcgacttcac cgagagttcc gcgaacatga ggccgacgtc ctggtcggaa
      961 cacagatggt ggcaaaggcg ctagatttcc ctctggtcac cctcgtcggt gtcgtgttag
     1021 ccgacatcac cctgcacctc cccgattttc gggcggcaga gcgtacgttc cagttgctgg
     1081 cgcaagtgtc tggtcgagct ggaagaggtt cgcgtgaggg agaggtgatt cttcagacct
     1141 actcgccgga tcattacagt gtagtcgcgg ccagccgcca cgactaccga gccttctacg
     1201 agagggagat tacctttcgg cgccaaaact catatccacc cttcagagaa ttggcgaggc
     1261 taacctacac gggcactggc gagacgcggg ctcgattcga ggcactcgag atgcgccagc
     1321 atctccggtc caagattgag gagatgggga tagctgatat cgagatcctg ggcccagccc
     1381 ccgccttcca cgctcgagtg cggggcaagt tcaggtggca gatcgttctg gctgggcaga
     1441 gacttgggcg tctgctatgt gagatcgacc tgccgctcgg ttggggcctg gacgtggatc
     1501 cgatgagtct gctgtaggga tctagaccct tgccgagcat gcacggatga gagcagtatc
     1561 aggctgttgg gttgtatttg cccttttgcg atgaggcgat cagcaacccc ctctgcacga
     1621 gcgttcgaat caactccagt ttgtatccgt ttcgcttcag cggcttcgca tccgctaacg
     1681 ctgcctcgcc tgcctcacga gccaggttcc cgtcgagggg tttccctcga accagatctt
     1741 ctgcttgagt tgcgcgccag ggaatgggag caacgccccc cataacgatg ttaatctccc
     1801 taggcattgt cccgtcccag gtgccctgca ctgctacgct gactagaggg aaggcccacg
     1861 tctctcgaga catcgctttg aggtatgccc cgcggcttcc gtctggaggg acgggaacgc
     1921 gaatctcggt tatcgcctcg tctggccgga ttatggtctc tcgacggcgt ccctcctctg
     1981 gcagtttgaa gaactgctcc accggcatcg ttcggcagct actctgtcgg ttgcagagag
     2041 tcatctgggc accgagtgcg ataagcgcct gaggtgggtc agacggatag gctgatcggc
     2101 aggggctgcc gccgaagagg gcgtgaagtt ggttttcgcc atcggcggcg taacatttct
     2161 cccctccctt gagccagcac aggaactgac ctcggaagta ccagcagcgg ctggtctggc
     2221 acaggttgcc gcccatcgtg gccatgttgc gtagttgcgg tgaagctgcc accgaaatcg
     2281 cttccgacag tattcgaaaa cgttggcgaa ctgccggcag cctctctacc tcggtgagtg
     2341 ttgccagagg ccctatcact agtcccccct catcgctgta gtcgagacgc gtgagttcgg
     2401 agatggtctt gacattgacc aggcgcgagg gggcgatgat gcgccgcttc atgagtggta
     2461 gcaggtcggt accaccggca atgagtcttg tttgccagtc ttcgcccagg gcggtgagca
     2521 gatcgtcagt ggaggttaca ttcagatgtt cgaaaggtct catggggtgg acctcttttt
     2581 cagcaacgtg cgaaccgaca tgggcaggtt gagaagacgt gtgcctgtgg cgttgaagac
     2641 agcgtttgcg atagcaggcg cggttggtat gatagggggc tctcctatcc cttttgcacc
     2701 caggttgttg ctgtgttgat ccggatcacc gattacagcc acctcgatgg atgacgtgtc
     2761 catcgaggtg gggatcaagt attcctccag gtcagggttc aacactcgtc cgttgcgagc
     2821 gtctatcact ctctcttcca tcactgagta tccgatccct tgagtaatgc caccctgtat
     2881 ctggttctcc gccagtttcg ggttgatgac ccgaccaaag tcgtggaccg ccacgatttg
     2941 cagcaggcgg atgcggccag tctcttcatc gacttccacc tctgcgaact gggcaccaaa
     3001 agtacggatt gcgtatcccg gtgggtttgg accgcgggag ccgcgcccaa ctatcatggc
     3061 gttgcccaga gatcccagca cctcactgac cggtcttcca ttttcggatg atccagccac
     3121 atagatgcga ttgtccttaa cctgcaactg atcgcgagat atctccatta cctgggatgc
     3181 gacttccaga agttcctctc ttgcgtcctc agctgctgcc ctgaccgcgg ggcctgtcga
     3241 ggaaatagtc tgactgcctc cactcgttgg actgtaggga gcgtctcggg tatccccttc
     3301 agaaacgtgc acgttttcga tggtgaagcc cagcgcttct gcggctatct gtgccaatcc
     3361 cgtcgcggtg cctgatccga tgtcctggga tcctgtcacc acatcggcag tgccatcggg
     3421 attcaacttg acgattgcat atgctggagg tccaccacct cctccccaga tctgggtggc
     3481 catcccgatg ccgagtttct tggtggtgaa gggagcgccc tggccagcct gggtagtacc
     3541 ggcagggtga cgttttgccc aaccggcctt ttcggctgcc aactggtaga cctctcggag
     3601 gtgctttgca gagtagtcgt gcccagttag aggatccttg tcggcgtagt tcttgagccg
     3661 caactcgaga gggtccatgc caagacgatc cgcgagcata tccatagccg actcgagggc
     3721 gaaggttccc tcagtgtaac ctggtgctcg gaaggctctg gcaggaccat cgttggtgta
     3781 cacggcgtac acttcggtgt ggacgttggg gcagtcgtat agctgcattg ccggaccggc
     3841 aatagatgct gcgtaaccgt atgcgccaac cggaaccacg cagtgcagat ggattgcggt
     3901 caagctaccg tcttgccttg caccaagctt caaggtctgt gccgttgggt gtctgtgccc
     3961 agtgatcagg ttctcctcgt gtctactcaa cacgatcctg acgggacgct ggatctgccg
     4021 agcgagcagc gccgcaagta ttgtctgctt gttgatctcc agtttgcttc cgaagccacc
     4081 gcctatgtaa tggctgatga ctcttactcg attgagaggc agaccgagta ttgctgcgat
     4141 ttccgctctt gcgacgaaca ctccctgggt cgagtcccag actgtcagat cctcgccaac
     4201 ccaattcgct acggtgccgt gagtctccat ggagttgtgg agtgccacct gggtcctgaa
     4261 ggtttcttcg acggttactt ccgcttgcga cagccccgcc ttcatgtcgc ctcgttcgta
     4321 gaccctgggt tttccatccg ccaggttgcc tgatggatgg actcgtggcg cgtcgggttg
     4381 catcgcctgc tctggatcaa gaacgaacgg tagttcctcg tattgaactc ggatgaggtc
     4441 gacggcgtcc tgtgccgtct cttcgtcgtc ggctgccacg actgcaatct catcgccaac
     4501 gaagttgagt accgtgtcca ggaccagttt tccatctttc cacctgatac ttggtgcatt
     4561 gatgggactt agtacccccc ttacccccgg cagcaactca gctctggatg tgtcgatgct
     4621 tacgattcgg gcatgcggat agggactgcg gaggattttg ccgtacacca tgcccgatgg
     4681 gtgaatgtcg ctggtgtatt gcgcacgacc cgtggccttt tcataggcat cgactcgatc
     4741 taccgatttc ccgacagtct gtagtttcgc ctcttgtgac catggctctg gctcgggtcc
     4801 ttctatgatc gtcaggtgct ctccaatgtt tccctcgtac tcaacttcgc tcttgaccac
     4861 cttgcccatc gttacctcct ccgccccatt cgctcggcgg ctcggagtgt ggcgcggaag
     4921 atgtttgggt atgcaccaca gcgacacaaa ttgcccgaga cagcccgctt cacctcgtcc
     4981 agggttggct ccagtcggtt atccagcaat cctttgatgg ccatgatctg acctgatgtg
     5041 cagtagccgc actggaaggc atcctcttct ataaaagctt cctggatggg atggagcttg
     5101 ccatcctttt ccagcccctc gatggtttcg atcctgtgtc cttcgcactc gatggcgagg
     5161 atcaggcacg cgtacacggg cactccatcc aacaagactg tgcatgcccc gcactgtccc
     5221 atgccgcacc cattcttggt tccggtcaga ctgagcgtgc tgcgcaggac ctccaataag
     5281 gttctgcgtg tttgcacggc tatgttgtac tcggccccgt tcactatgag gctgatggtt
     5341 gttttgcctg gacctcttgg caccagccaa gaagggcgtt tgccggcgag ttcggtcatt
     5401 gaatgcctcc aatcaggaca gttacaggtc aacgcgtatc tgcttca
//
LOCUS       DATALS010000019         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_362182, whole genome shotgun sequence.
ACCESSION   DATALS010000019 DATALS010000000
VERSION     DATALS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362182"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VHS06_00390"
     CDS             complement(<1..744)
                     /locus_tag="VHS06_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HEX2986625.1"
                     /translation="MAHELDSDTRRGDSIGEYVLLVGQSPLAPAPPSSQSPGALTVEI
                     RLDPIDVIATSVNCRELTPFATTLLREILEGRRLEPALRECEGVLQRRYFGPNKAKLL
                     QAMKEVRSQFNSWSQSMRLLEPYSAGRDAPSDPFDAVRPPEGPHPSNGSHHVAPNDRD
                     KERVSVQFSVPDGSQQPILLDAPRSPLEATDPVAGAPMLAQGAPRTGSVHTNVHHNLA
                     TLLVTLLAHSRLLDGKGWPHSPAAPVPAET"
     gene            complement(1024..>1566)
                     /locus_tag="VHS06_00395"
     CDS             complement(1024..>1566)
                     /locus_tag="VHS06_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986626.1"
                     /translation="RPQPTPIHTNCRAASSGDRRLIVTKPTDTSIRTMGSALGYAGDQ
                     IRGHPLITGSVVLAMAGAFIGSRIAQARAMRRQKSPLQRAQDLMGPIGFMALAAIARQ
                     RMRARGKGMAASALGISGPIMESLPNLAIQRRRRVGAPNAIRQAGYALSLIPVTIAFL
                     RNPLVRDMGFRMMSRRIGRR"
BASE COUNT          261 a          456 c          539 g          310 t
ORIGIN      
        1 tgtttccgct gggacgggtg cagcagggga gtgcggccat ccctttccat ccaatagcct
       61 ggagtgggcc agcagtgtga ccagcagcgt tgcgagattg tggtggacgt tggtgtgaac
      121 cgatcctgtc ctgggcgcgc cttgggcgag catcggagca cccgcgacgg gatcggttgc
      181 ctccaggggc gatcgtggtg catcgagaag gatgggctgc tgagatccat cgggcacact
      241 gaactggacg gacacacgct ccttatcgcg gtcgttgggg gcgacgtggt gcgacccatt
      301 ggaggggtga ggcccttcgg gcggacgcac agcgtcgaaa ggatcggagg gtgcgtccct
      361 gcctgcactg tatggttcca gaagcctcat gctttgggac caggagttga actggctgcg
      421 aacctccttc atcgcctgga ggagctttgc cttgttggga ccgaagtagc gccgttgcag
      481 aacaccctcg cactctctca gcgcaggctc gagtcggcgc ccctccagga tctcccgcag
      541 tagggttgtg gcgaacgggg tcagctctcg acagttgacg ctggtggcga tgacgtcgat
      601 ggggtccagg cggatctcga ccgtcagagc cccgggggat tgagaggatg gaggagctgg
      661 cgctaagggc gattgcccga ccagcagaac gtattcgccg atggagtcac ccctccgagt
      721 gtctgagtcc agctcgtgtg ccaacagctt cgcctttctc gagcccgggg acacgctccc
      781 cggtggacct tgccggaaac cggcaatgca agaccgtgtg aagacgcgtg cgcaacggtg
      841 cgaggctcat gccaagggta ctgatggggt ggcaggacac cacggactga agtgtctaga
      901 ttatagcagc tatgtggttg atgggacagc ttggtttgtg accagcgact cggggaaacc
      961 gctgaggcgg gtctgggggg cgacccgccc ctgttggccg tgcggtgcca ccgccgtggc
     1021 catctaccgg cggcctatcc gcctcgacat catcctgaag cccatatctc gaaccagcgg
     1081 gtttcgcagg aacgctatag tcaccggaat caggctgagg gcgtaaccgg cctgcctgat
     1141 ggcgttaggt gccccgactc gacgccggcg ctggattgcc aggttgggca ggctctccat
     1201 gatgggtccg cttatcccca gggcggaagc ggccatccct ttccccctgg cgcgcatcct
     1261 ttgtctggcg attgccgcca gggccatgaa cccgatgggg cccatgaggt cctgggctcg
     1321 ctggagcgga gatttctggc gtcgcatagc ccttgcctgg gctatgcgag atcctatgaa
     1381 tgcgccggcc atggcgagaa cgacggaacc ggtgatcagc ggatgacctc tgatctggtc
     1441 cccggcgtag ccgagcgcgc ttcccatcgt cctgatgctg gtatccgtcg gtttggtcac
     1501 tatcagccgc ctgtcgccgg aactggctgc ccgacagttc gtgtggatcg gggttggctg
     1561 cggtct
//
LOCUS       DATALS010000020         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_1952,
            whole genome shotgun sequence.
ACCESSION   DATALS010000020 DATALS010000000
VERSION     DATALS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..210
                     /locus_tag="VHS06_00400"
     CDS             55..210
                     /locus_tag="VHS06_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2986627.1"
                     /translation="MSFQIKPGQLAALVGPSGAGKTTITYLLPRTPNRARLTWPSPPG
                     PSPSRQY"
     gene            complement(164..379)
                     /locus_tag="VHS06_00405"
     CDS             complement(164..379)
                     /locus_tag="VHS06_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986628.1"
                     /translation="MYRLAYRMADLGYVVGPMLLGWLAAVSGNETSLLVTAAMFLVSR
                     MLFAIFAPESSMVNIAAKVKVPAGMAK"
     gene            complement(372..>1755)
                     /locus_tag="VHS06_00410"
     CDS             complement(372..>1755)
                     /locus_tag="VHS06_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2986629.1"
                     /translation="RTVTRDGMNMNAGMNAMMNETLNVSGALLVKIFGRERDENRRFQ
                     ERAAGVRDVGIRQAVIGRWFFMGLSLISSVGTALVFWWGGMLVLHGEFTVGTIVAFGA
                     YLTQLYGPLTSLTNARVEFATSMVSFERVFEVLDLPQEIFDRPGAVTLDRVAGDVRFE
                     NVSFSYRDGDGVPGVFGLSEVQRWHWGGDAVPVTKSPRHKGSSDRANGDSHGGNGRDG
                     VEEATSGSLRNWSLRDVSFQIKPGQLAALVGPSGAGKTTITYLLPRLYDPTGGRILID
                     GVDIRDITLESLSSQIGMVTQESYLFHDTIRANLLYARPNATQEQLEDACRAANIHDF
                     IVGLSDGYDTVVGERGYRLSGGEKQRIAIARVILKDPRILVLDEATSHLDSHSEALIQ
                     AALERVMVSRTSLVIAHRLSTILAADLILVIDEGRLVEQGTHHELLARGGLYADLYET
                     QFRERAEESV"
BASE COUNT          352 a          610 c          472 g          321 t
ORIGIN      
        1 gacggcgtag aggaggctac ttccggctcg cttcgaaact ggtcgctgcg agacgtgagc
       61 ttccagatca agcctgggca gctagccgcg cttgtgggcc ccagcggggc cggaaagacg
      121 accatcacat atctattacc ccgaacaccc aatagggcaa gacttacttg gccatccccg
      181 ccgggacctt caccttcgcg gcaatattga ccatggagct ctccggcgcg aatatcgcga
      241 agagcattct ggacaccaga aacatggcgg cggttaccag caacgacgtc tcgtttcccg
      301 agacggccgc caaccaccct aacaacatcg gccccaccac gtaccccagg tcggccatcc
      361 tgtatgcaag tctatacact ctcctccgcc ctctcccgaa actgcgtttc gtacagatcg
      421 gcatacagcc cgcctcgggc cagcaactcg tgatgagtcc cctgttccac caggcgaccc
      481 tcgtcgatga ccaggatcag gtccgccgcc agtatggtgc tgagccggtg ggcgatgacc
      541 agactggtgc ggctaaccat caccctctcc agggcagcct ggatcagcgc ctcagagtgc
      601 gagtccaggt gggaggtggc ttcgtccagg actagtatcc ttggatcctt caggatcacc
      661 ctggcgatgg cgatcctctg cttctcgccg cccgaaagtc ggtagccgcg ctcgccgacc
      721 accgtgtcgt acccatcgga caatccgacg atgaagtcgt ggatgttggc ggcccgacag
      781 gcatcctcca actgctcctg cgtggcgtta gggcgtgcat acagcagatt ggcccgaatg
      841 gtatcgtgga agagatagct ctcctgcgtg accatgccga tctgcgagga aagcgactcc
      901 agagttatgt cacgaatatc cacaccgtcg atcagtatcc gcccgccggt gggatcgtac
      961 aaccgaggca atagatatgt gatggtcgtc tttccggccc cgctggggcc cacaagcgcg
     1021 gctagctgcc caggcttgat ctggaagctc acgtctcgca gcgaccagtt tcgaagcgag
     1081 ccggaagtag cctcctctac gccgtctcga ccattccccc catgagaatc gccgttcgcc
     1141 cgatcactac tccctttatg tcgcggactc ttcgtgaccg gcacagcgtc gcctccccaa
     1201 tgccaccgct gcacctccga taggccgaac acgcctggaa ctccgtcccc atctctgtaa
     1261 ctgaaggaga cattctcgaa gcgaacgtca cccgccacgc gatcgagggt caccgcgccc
     1321 ggtcgatcga agatttcctg gggcagatcc agcacctcga agaccctctc gaagctgacc
     1381 atggaggtgg cgaactccac ccgcgcgttg gtcagcgacg tcagcgggcc atacagctgg
     1441 gtcaggtagg ctccgaaggc cacgatggtt cccacggtga actcgccgtg cagcaccagc
     1501 atgccccccc accagaacac cagcgccgtc ccaacagagc taatcaggct cagccccatg
     1561 aagaaccacc ggcctatcac ggcctggcgg atacccacat cccgcacgcc cgccgcccgc
     1621 tcctggaacc gccgattctc gtctcgctcc cggccgaaga tcttcaccag cagcgcaccg
     1681 ctgacgttca gtgtctcgtt catcatcgcg ttcatgccgg cgttcatgtt catgccatcg
     1741 cgagtcacgg tgcgc
//
LOCUS       DATALS010000021         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_362358, whole genome shotgun sequence.
ACCESSION   DATALS010000021 DATALS010000000
VERSION     DATALS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362358"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VHS06_00415"
     CDS             complement(<1..544)
                     /locus_tag="VHS06_00415"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX2986630.1"
                     /translation="MFIRSRTGDLVSGVIALLLGVFYLYEGTHYRLWTAGGNPGGGFL
                     PTVLGGSMVLLSVVLITKTLRAQPSEGGTVEVSFNKPLLVLGALAFYVVLLPFLGYLL
                     ASTVVTAFLLFTFDDGDSRRRVVKSVVVSVAVVAIFYVVFVQVEHPQQQGDDAAHQVT
                     SSAPDKHELPPIPIRMAASAR"
     gene            complement(599..1750)
                     /locus_tag="VHS06_00420"
     CDS             complement(599..1750)
                     /locus_tag="VHS06_00420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX2986631.1"
                     /translation="MATEIRMPRLSSSMLEGRIVRWYKRVGDRVNQGEALLEIESDKA
                     VSDVEASASGVLLQIAAGDDSVVDVDGLLGAIGEEGEAGVPATAATQAAAAQTVGAAP
                     GVQPSLQRAGKVKASPVAKRLAAKFGVSLDDIVGSGRDGLIVEADVLAARDAAPSSMG
                     EGEDEVGDVVSLTGIQAAMLRHMVEVGRNVVQATTFAEVRMLRAMEARRQAKISITTL
                     VAWAAVRAIQDFPVINSCLQEGNRLLLRKQVNLSVAVASPKGLVVPVIRGAHRLGIQE
                     LSNQVESLAERARVGQLAPSDFEGGSFTLTNSGSLGALLSAPVVNYPEVAILAAGKVA
                     DWPIAVDGQVVVQPLMYLSLSYDHRVIDGAAAIGFVQKVKRLLEEQPLG"
     gene            complement(1800..2405)
                     /locus_tag="VHS06_00425"
     CDS             complement(1800..2405)
                     /locus_tag="VHS06_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2986632.1"
                     /translation="MPCSANVFAADNAFRTIKIVMPSTPADAKGLLKSAVRDPDPVLV
                     FEHKLLYGVRGEVPDGTEQLVPIGKAATARPGSDLTIVAVGMMVRKALSAANTLAEQG
                     IEAEVLDLRSVRPMDTEAIVESVSRTGRLVIVHEAPVTFGIGAEIAAVVAKEAFGYLD
                     APIERVGAPDTPVPFSASMEDFYLPNEQKIVEAALRTTAAA"
     gene            2353..2473
                     /locus_tag="VHS06_00430"
                     /pseudo
     CDS             2353..2473
                     /locus_tag="VHS06_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514246.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
BASE COUNT          504 a          862 c          668 g          439 t
ORIGIN      
        1 gtctcgcgga cgctgccatt cgtattggga ttggaggtag ctcgtgttta tcaggagccg
       61 aactggtgac ctggtgagcg gcgtcatcgc cctgttgctg ggggtgttct acctgcacga
      121 acaccacata gaagatggcc acgacagcca cgctcacgac caccgacttc accacccttc
      181 tcctcgaatc cccatcatcg aaagtgaaga ggaggaatgc agtcaccact gtgctcgcga
      241 gcaggtaacc caagaacgga agtagcacca cgtagaaagc tagcgcccca aggaccagga
      301 gcggcttatt gaaagagacc tcaacagtcc cgccctcgga tggctgcgcg cgcagcgtct
      361 tggttatgag gactacggag agcaggacca tggagccccc aaggacagtg ggcagaaaac
      421 cccctcccgg attaccgcca gcggtccaga gccggtaatg ggtgccttcg tacaggtaga
      481 acacccccag caacagggcg atgacgccgc tcaccaggtc accagttcgg ctcctgataa
      541 acacgagcta cctccaatcc caatacgaat ggcagcgtcc gcgagaccct cgcctcggct
      601 atccaagcgg ctgctcttcc agcagccgct tcaccttctg gacaaaaccg atcgccgccg
      661 ctccatctat caccctgtgg tcgtaggaga gggaaagata catgaggggc tgcaccacca
      721 cctgcccgtc cacggcaatc ggccaatcag ccaccttgcc cgcagccagg atcgccacct
      781 ccggatagtt caccacgggg gcagagagca gcgcccccag cgagccggaa ttcgtaagcg
      841 tgaagctgcc accctcgaaa tcgctggggg caagctgccc gactctcgcc ctctccgcca
      901 gcgactccac ctgattcgac aactcctgga tgcccaggcg gtgggctccc cgaatcaccg
      961 gcactaccaa ccccttggga gacgccacgg ccacgctgag gttgacctgc ttcctgagca
     1021 gcaaccggtt gccctcttgc agacaggagt tgatgactgg gaagtcctgt atcgcacgga
     1081 ccgcggccca ggccacgagc gtcgttatgg agatcttcgc ctgccgccgc gcctccatgg
     1141 cccgcagcat gcgcacctcg gcaaacgtcg tcgcctgcac cacatttcgc ccgacctcca
     1201 ccatgtggcg tagcatcgca gcttggatcc cagtaaggga gactacatcc cccacctcat
     1261 cctcgccctc acccatcgaa gatggggctg cgtcccgagc cgccaacaca tccgcctcga
     1321 cgatcaagcc gtcgcggcca ctacctacta tgtcatccaa cgacacgcca aacttggccg
     1381 caagccgctt cgccacaggg gatgccttga ccttgcctgc cctctgaagg ctaggctgca
     1441 caccaggggc ggctcccacc gtttgcgctg ccgctgcctg agtagccgca gtagccggca
     1501 cccccgcctc gccctcctca ccaatggctc ccagcaaccc atccacatcg accacggagt
     1561 catcgccggc agctatctgg aggagtactc cactggcaga cgcctcaaca tcggagactg
     1621 ccttgtcaga ctcgatctcg agcagcgcct ccccctgatt gactcgatcg cctaccctct
     1681 tgtaccaacg aacgatgcgg ccctccaaca tgctggagct gagccgcggc attcggattt
     1741 ctgtcgccat gatccctcct ggagcggtgt gggcggagga cccgcccata tgccgatcgt
     1801 tatgctgcag cagtcgtccg caaagctgcc tcgactatct tctgctcgtt cggcaggtag
     1861 aagtcctcca tcgatgcact gaacggcacc ggggtatccg gagcgcctac ccgctcgatc
     1921 ggggcatcca gatatccaaa ggcttctttg gcaaccaccg ctgcgatctc tgcacctatt
     1981 ccaaaggtga ctggggcctc atggacgatt acgagccgtc cggttctgga gaccgattcc
     2041 acgatggcct ccgtatccat cggacgcaca ctcctcaggt ccagcacctc agcctcaatg
     2101 ccctgctccg ccaacgtatt cgccgcggac aacgccttcc gcaccatcat ccccacagcc
     2161 acgatcgtga gatctgaacc gggtcgcgct gtagctgcct tcccgatggg caccagctgc
     2221 tccgttccat ccggcacctc accacgcacc ccatatagca gcttgtgctc aaagaccagc
     2281 accggatcag ggtcccgcac ggccgacttc agcaatccct tggcatcggc cggcgtggag
     2341 ggcatcacga tcttgatggt gcggaaggcg ttgtccgcgg cgaatacgtt ggcggagcag
     2401 ggcattgagg ctgaggtgct ggacctgagg agtgtgcgtc cgatggatac ggaggccatc
     2461 gtggaatcgg tct
//
LOCUS       DATALS010000022        11614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_137116, whole genome shotgun sequence.
ACCESSION   DATALS010000022 DATALS010000000
VERSION     DATALS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137116"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..149)
                     /locus_tag="VHS06_00435"
                     /pseudo
     CDS             complement(1..149)
                     /locus_tag="VHS06_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252465.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
     gene            413..1423
                     /gene="trxB"
                     /locus_tag="VHS06_00440"
     CDS             413..1423
                     /gene="trxB"
                     /locus_tag="VHS06_00440"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018930967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX2986633.1"
                     /translation="MMKLLPPSCYNGLNLPHIAAQEELEMEQYEVVIVGGGPAGLTAG
                     LYTSRAKQRSLLIEGKWPGGQLRNTELIEDYPGFESIKGAELADRMENQVRKLGLEIL
                     LDNVICIRPEGNWKRVQTENGKEFLAKAVIVTSGGEPRRLGVPGEEELAGRGVSYCAI
                     CDGAFFEDMDVAVVGGGDSAVEEAVFLTRFASKVYLMHRRSEFRAQSVAVERATSNSK
                     IEIMLDTTVESINGADEVESLTITRRGESQELKVSGVFIFVGFMPNTELFCGHVEHDP
                     RGYIVTNDSMETSVRGIYAAGDVRAQLARQITTAVGDGTTAAIAAGQFIENFEDIETI
                     DV"
     gene            complement(1541..2221)
                     /locus_tag="VHS06_00445"
     CDS             complement(1541..2221)
                     /locus_tag="VHS06_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2986634.1"
                     /translation="MSLISKEDREFLVDHFAKELTAPVKIVYFTQPSSNLLLPGGATS
                     GCEYCEDTQGLLEEVASLSDKITLEVHDFATDKALAEQYGVNKVPATVILGAARGKIR
                     FFGIPSGYEFGSLIEGIVDISRGTTDLSDATKKLLDSVTEDLHIQVFVTPTCPYCPSA
                     ARMAQKMAIENEHVIADTVEATEFPQMSRQYKVQGVPKIVVNEDTQFVGALPEPRYLG
                     EVMKAVKA"
     gene            complement(2264..2479)
                     /locus_tag="VHS06_00450"
     CDS             complement(2264..2479)
                     /locus_tag="VHS06_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009767579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX2986635.1"
                     /translation="MDCNVGGIDRLARIVLGIGIVAVGVATNSWLGIVGLALAGTGVG
                     SRCLLYRPLGLSTCGSKGGSEKVGAQR"
     gene            complement(2570..3646)
                     /gene="selD"
                     /locus_tag="VHS06_00455"
     CDS             complement(2570..3646)
                     /gene="selD"
                     /locus_tag="VHS06_00455"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(3569..3571),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX2986636.1"
                     /translation="MRSRDRENRTRAATVKLTSFSHGTGUACRIGQEDLAQVLRHLPK
                     YDDPRLLAGRDTLDGGAALQIRDDLVLVQSAAFITPVVDDPYSFGAIAVADAMSDLYA
                     KGARPILGLNLVGFPSHTLPISTLGEILKGGAGKATEAGAMVMGGHSLDDPEPKYGMA
                     VTGLADPRSLVSDAGGRDGDLLVLTKPLGSGIAATAIDRRLASQGLVDRAVALMSELN
                     RAASEAMLEVGVDGCTDVSGFGLLGHLHEMAKASGLSASVELGKVPVLEGVWDLVRQE
                     VVPEGTHTNHRYLQGKVDWGDATRDEQLVLSDSQVSGGLLIACPPERVDLLLARLAAM
                     GVSAALIGRLADGGAGKITVTRGQ"
     gene            3691..4614
                     /locus_tag="VHS06_00460"
     CDS             3691..4614
                     /locus_tag="VHS06_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX2986637.1"
                     /translation="MDKAYSESVIALNLYHLRVFLAVARRLSFSRAAEELFISQPAVS
                     RHVHDLEKEIDATLFGQTGNRVYLTEAGRLVYGYAQRLFDVEAEMMRALRELEEMDRG
                     TLRLGASDTVGTYLLPPVIAAFQQRYPRVEVSLSIDHDGEIERMLLENDLDLGFAVSA
                     EPPGLQARPYAGDRLVLIVPPGHRLAEFDVVGGKELEEETLLLREAGSSSRMLLEAEL
                     ARAGITPRRTIELRGCEATKRGVIAGMGVAVVSGHSIEEELRHGSLTAVDLEGLKLER
                     EISIISRKEARPSVSALTFTAMALKASTPTR"
     gene            complement(4679..5578)
                     /locus_tag="VHS06_00465"
     CDS             complement(4679..5578)
                     /locus_tag="VHS06_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX2986638.1"
                     /translation="MNLEQLRSFRAVAQEGGFSRAADALFLTQSTVSMQVATLERELG
                     VKLFHRMGRRVVLSHAGESMLGYAGRILSQVDESRKTMASFRGLAAGELLVGASLTIG
                     SYLLPELFGRFHKLHEGVRLLVDIAPTPRQVEQIVSGKMDLGLVEGPVGSPELVVEPF
                     FIDQLALIVPPEHRWVGRSSVAVEELQEEPFLEREPTSGTREIVHGRLAEYGIELKPV
                     LELGSPEALKRAVRAGLGVAIVSRATLELELRTGLLVSLHVSGVELTRPFYTVVHKDR
                     YVSPPLAAFLELLHATYIVERSQ"
     gene            5765..6610
                     /locus_tag="VHS06_00470"
     CDS             5765..6610
                     /locus_tag="VHS06_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2986639.1"
                     /translation="MDPTQLFAALFASSVAAGVLGSLLGLGGGIIVIPILTLLLGVDI
                     RYAVGASIVSVIATSNGATASHLRDGIANLRVGMLLETATATGAMIGALATAMIGGRP
                     LYLIFAVVLGVSSVAMFWRRNADDCPNPVSHPWAERLRLGSSFPDRSTGRTIRYGVAG
                     VPVAMGLMLMAGVVSGLLGVGSGTLKVPAMDLAMRLPVKVSTATSNFMIGVTAAAGAA
                     VHFTRGDVQPLLAAPVALGVLVGAVVGARTLGRLHSSSVRTLFVLVLVVVSVQMAARG
                     LGFQI"
     gene            6623..7093
                     /locus_tag="VHS06_00475"
     CDS             6623..7093
                     /locus_tag="VHS06_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEX2986640.1"
                     /translation="MMAYAKGRDSDNRATAEARLVPTEVTIHGDSTAPSSIAKNGRDK
                     RLEGVVGPVLRIGSLISMALILTGLALTVLGWPVTRLIASPAPTLGSVFQGPGGTFPT
                     TLINLGILVLIATPVARVASTVVFFLARGELSYLAITAYVLMVLLAGFLLGATG"
     gene            complement(7090..8358)
                     /locus_tag="VHS06_00480"
     CDS             complement(7090..8358)
                     /locus_tag="VHS06_00480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2986641.1"
                     /translation="MTQFASIAVQAAASGISFVGRQSRDWRVTVARTSLDRLVYQMVF
                     PYLSIYIVALGATATQLGGINSAGMVAAAALGPSTGWLIDRIGAKRVYLAGIVMLAFS
                     YLTYSLAQNWQMALVAMVAYWLGYSTSVLGCATVCGNCLATRDRATGMMICETVGAGL
                     LGMAGPLIGAWLVAGSGGVAVDGIRPLFVVSTIVTLGSFVLVQTQLSDRRWVNVRGAP
                     GRMFKDLHQVLKDGRHLKRWLVITSVGQLPLAMVFPFAQVYAHSTKGADAYVLGVMVT
                     GCAVTSIVLAIPLGRLADRWGRKAILLAVTPLFWVSNLVLVWAPDSAFLIVAGILQGF
                     YYISGPIGAAMERELVTADQMGRWLGITRFFRMLLSALMAGVAGIMWDRVGPQYVFLG
                     FVAVDLVVRLPLLLTMPETLQSKLVVEARP"
     gene            complement(8427..9062)
                     /locus_tag="VHS06_00485"
     CDS             complement(8427..9062)
                     /locus_tag="VHS06_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HEX2986642.1"
                     /translation="MAECAECKVYVCRIGHAEVAPVNCPMRDGDVTNTCESLSDTTLR
                     RMAHESALVESEGYLRWTRVEETMEFARRMGYEKLGIAFCVGLREEAKVLSRVLKANG
                     FKVASVVCKTGAIPKEELDIADCQKVHPGEFEAMCNPIGQARVLNREKTDFNIVFGLC
                     VGHDSLFIMHSKAPVTCLVAKDRVLAHNPIGALYGSEGYFKKALYETHKPA"
     gene            complement(9163..9384)
                     /locus_tag="VHS06_00490"
     CDS             complement(9163..9384)
                     /locus_tag="VHS06_00490"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX2986643.1"
                     /translation="MVGIAWPVYCPDTGHHHEIFGPSRGEELARAAGAPLLARLPLDP
                     AVAPLCDAGRLEEYASESIDSLAAAFWGC"
     gene            complement(9372..10163)
                     /locus_tag="VHS06_00495"
     CDS             complement(9372..10163)
                     /locus_tag="VHS06_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEX2986644.1"
                     /translation="MTLQSLRDAAAAIESILRLKSFPLAVKMLRSEDEIPEGAKRPVR
                     DLGRHLSTCQGFALSRRDGLTVAQTKEDMWCFEPVLGYGLAKPPKLFLEGQNQIPKAA
                     RTLEVGRTWVEALPRLALGEYIGVVSAPAKEATFEPDLVIVYCDPSQLTQLLMVKDWM
                     DGVDVASKLSGHAACIYAVVPTLQTGEWSVASPCRGDRARALASDDEIAVSIPAAQLP
                     ETRDGLIHFGEYESGLPLALSMLHEYPLAPGYVKIGRMIGMDWLE"
     gene            complement(10254..11174)
                     /locus_tag="VHS06_00500"
     CDS             complement(10254..11174)
                     /locus_tag="VHS06_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HEX2986645.1"
                     /translation="MRSLLAVRCSGAGGKITVSRPRKPRCVGLLPDVRYFKPQGTPMR
                     EPKEVQLSLDELEALRLKDLEELDQEESARRMGVSRPTFQRVLDQAHFKVAEALVDGK
                     ALRIEGGDYQVVPSRFHCPGCGHGWEQVMSSRASVTCPHCHKAAANESESGRVDPLYA
                     MASTSRAQIREALAKGDRMKIAAVSDDGKTICAHFGRAKQYVVLTVEGGRVVEREVRD
                     RSSQLRIVHPQGDGELHQHIHGHGHDHNAMAAAIKDCTIVLAGGMGRPAYASLKANGL
                     EPVITDIVDIDEAVQAFISGAMENHTERLH"
     gene            complement(11308..>11614)
                     /locus_tag="VHS06_00505"
     CDS             complement(11308..>11614)
                     /locus_tag="VHS06_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986646.1"
                     /translation="LVVFFLPYGVARATGHWSTSGKFDGSGTPITATGRDTAEIKGWM
                     TFGEISRGYNVSLEEIADNFKLPREKVTADQVMNKVESEDFSVSKLREWLDSRKTKK"
BASE COUNT         2291 a         3863 c         3345 g         2115 t
ORIGIN      
        1 accttctccg gccttctgat ggcattgagc ccccagaagg tgaagaacat ggtgaccggc
       61 gtatccatgg cggcggctcc gttggcgatg atgaaggccg ccaaaacctt gtcgagatcg
      121 cccgagaaga cgatcatcga cagaccatct tcagtgggag tctcctcctc cacggcggga
      181 gcctccgtgt cggaaacacc atccacaacc atcttccacc tccccacaag aaaggactcg
      241 gcgttgagca aaccgatccc cacccactgc aacatggggg ggctcgacca gccatcagcc
      301 aagccctcga tggcatcacg ataccatacc caggtattgt attgcaatag tgaaattcgg
      361 agcgaactgt tccggacgcg gaaaggggcg gcggggcaag gaggttgggt cgatgatgaa
      421 attgcttcct ccttcttgct acaatgggtt gaacttgccg cacatcgcag cacaagagga
      481 gctggagatg gagcagtacg aggtcgtcat cgttgggggc gggccggcag ggctaaccgc
      541 cgggctgtac acctcccgcg cgaaacagag gtcgctactg atcgaaggaa agtggcccgg
      601 aggccagctg cgcaacaccg agctgatcga ggactacccc gggttcgaaa gcatcaaagg
      661 cgccgaactg gccgacagga tggagaacca ggtccgcaag ctagggctgg aaatcctgct
      721 ggacaacgtg atctgcattc gccccgaggg caactggaag agggtccaga cggagaacgg
      781 caaggagttt ctggccaagg ccgtgatagt gaccagcggc ggcgagccca gacggttagg
      841 tgtgccgggc gaggaggagt tggccggcag gggcgtatcc tactgcgcca tctgcgacgg
      901 ggctttcttc gaggacatgg atgtggcggt ggtcggcggc ggggactcgg ccgtcgagga
      961 ggcggtgttc ctcacccgct tcgccagcaa agtgtacctg atgcatcggc gcagcgagtt
     1021 cagggctcag agcgtcgccg tcgagagggc aacgtccaac tcgaagatcg agatcatgct
     1081 ggataccacg gtggaatcga tcaacggagc cgacgaggtg gagtccctca ccatcactcg
     1141 tcgaggcgaa agccaagagc tgaaggtatc gggcgtgttc atcttcgtgg gcttcatgcc
     1201 caataccgaa ctgttctgtg gccacgtcga gcacgacccg cgcggctaca tagtcaccaa
     1261 cgactccatg gagacctccg tcagggggat ctacgctgcc ggtgacgttc gtgcccaact
     1321 tgcgcggcag atcaccacgg ccgtcggcga cggcaccacg gcggccatcg cggccgggca
     1381 gtttattgag aatttcgagg acatcgaaac catcgacgtc tagccaggga atcgtagggc
     1441 ggggtcttgt gccccgcccc ccacaccatg aataacgacc gccacggtga atccagccca
     1501 cgctccaggc ggggcacaag accccgctct acgtcgtagt ctaggccttg acggccttca
     1561 tcacctcgcc gaggtaacgc ggctccggca gcgcacccac aaactgagtg tcctcgttca
     1621 ccacgatctt gggaacgccc tgcaccttgt actgccggga catctgcgga aactccgtgg
     1681 cctccaccgt gtcggctatg acgtgctcgt tctctatggc catcttctgt gccattcgcg
     1741 cggcgctcgg gcagtaagga caggtgggag tcacgaacac ctggatgtgc agatcctccg
     1801 tcaccgagtc caacagcttc ttcgtggcgt cgctgaggtc cgtcgtgccc ctggagatgt
     1861 ccacgatccc ctcgatcagc gacccgaact cgtaccccga agggatgccg aagaagcgga
     1921 tcttgcccct ggccgcccca aggatgaccg tggcagggac cttgttcaca ccgtactgct
     1981 cggcaagggc cttatcggtc gcgaagtcgt gcacctcgag ggtgatcttg tccgacagcg
     2041 aggctacctc ctccagcagc ccctgagtgt cctcgcaata ctcgcatccg gaagttgccc
     2101 ctccgggaag cagcaggttc gatgacggct gcgtgaaata gactatcttc acaggagccg
     2161 tgagctcctt ggcaaaatgg tcgacgagga actcacgatc ctctttggaa atcagcgaca
     2221 tatctcttcg cctctcctta cttctctcgt tgccggcgcg gggttaccgc tgcgccccga
     2281 ccttctcgct cccgcccttt gacccacagg tggaaagccc aaggggcctg tacagcagac
     2341 acctgctgcc cacacccgtc ccggccaggg ccagtccgac gattcccaac caggagttcg
     2401 tggcaacccc aaccgctacg atcccgatgc ccaggacaat cctcgccaac ctatcaatcc
     2461 caccgacgtt gcagtccatc tcagtcaatc cccctgtact acatgcgagg ccgggcaacc
     2521 ggccaccgct caaccttcca atcagaccca atatggggca tgaagccatc tactgccccc
     2581 tcgtaacggt gatcttgccg gcgcccccgt cagccagccg tccgatcagc gccgccgaca
     2641 cacccatcgc agccaaccga gccaggagca gatccactcg ctccggcggg caggctatca
     2701 gcagtccgcc cgaaacctgc gagtcactca gcaccaactg ctcgtcccgg gtcgcatccc
     2761 cccaatcgac ctttccctgc aggtatcgat gattggtgtg ggtgccttcc ggaaccacct
     2821 cctggcgaac caggtcccac accccctcca gcaccggcac cttcccaagc tctacggatg
     2881 cggagagacc tgaggctttc gccatttcgt gcaggtggcc cagcagaccg aaacccgaca
     2941 cgtcggtgca accgtccacc ccgacctcca acatggcctc ggaggccgcg cggttcaact
     3001 ccgacatcaa cgccaccgcc cgatccacca gcccctgcga ggccaaccgt cgatctatgg
     3061 ccgtggccgc aattccactg cccaacggct tggtcagcac caggagatcc ccgtccctgc
     3121 ccccggcgtc gcttaccagg ctgcgaggat ccgccagccc ggtgaccgcc atcccgtact
     3181 tgggctccgg gtcgtccagg gaatgcccgc ccatcaccat agctcccgcc tcggtcgcct
     3241 tgcctgcccc gcccttcagg atctctccaa gggtcgatat cggcagcgtg tgggaaggga
     3301 accccaccag gttcagaccc aggatcggcc tggccccctt tgcgtacaga tcggacatag
     3361 cgtccgccac ggcgattgcc ccgaagctgt agggatcgtc caccaccggg gtgatgaacg
     3421 ccgcgctctg gaccagcacc aggtcgtccc tgatctgaag cgcggccccg ccgtccagcg
     3481 tgtcgcggcc tgcaagcagc cttggatcgt cgtacttggg taggtggcgc agcacctgcg
     3541 ccaggtcttc ctgacctatc ctgcaagctc aaccggtgcc gtgggaaaag gatgtcaact
     3601 tcactgttgc cgccctcgtt cggttttctc tatctcggct tctcatggcg caaggctatc
     3661 actacgaatg ctataatgcc aatacaaaga ttggataaag cctattcgga aagtgttatc
     3721 gccttgaacc tgtatcacct gcgagtcttc ctcgccgttg ccaggcggct ctccttctcc
     3781 agggcggccg aggagctgtt catcagccag ccggccgtct cccggcacgt ccacgatctg
     3841 gagaaggaga tcgacgccac cctcttcggc cagacgggga acagggtcta cctcaccgag
     3901 gcgggcaggc tggtgtacgg ctacgcccag aggctgttcg acgtcgaggc ggagatgatg
     3961 agggcgctgc gcgagttgga ggagatggat cgcggcactc tccggctggg cgcctcagat
     4021 accgtaggca cctatctgct gccccccgtg attgcggctt ttcagcagcg ctaccccaga
     4081 gtcgaggtga gtctctccat cgaccacgat ggggagatag aacggatgct gctggagaat
     4141 gacctggacc tgggcttcgc tgtgagtgcg gaaccgccgg gtctccaggc gcggccatac
     4201 gccggcgata ggctggtgct gatcgtgccg cccggccacc gccttgcaga attcgacgta
     4261 gtcggaggca aggaactgga ggaggagact ctgctccttc gagaggccgg atcgagcagc
     4321 aggatgctcc tggaggctga gctggcaaga gccgggatca cgcccaggcg aaccatcgag
     4381 ctgaggggat gcgaggcaac gaaacggggc gtgatcgcgg ggatgggtgt ggcggtggtc
     4441 tccggccaca gcatcgagga ggagcttcgg cacggctcct tgacagccgt cgacctggaa
     4501 ggattgaagc tcgaacgaga gatcagcatc atctcccgca aggaggcccg gccctcggta
     4561 tccgcgctca ccttcaccgc catggcgctg aaggcttcca cgcccactag gtagccatca
     4621 agatccaacc gctacggcca tcgccccgca cagggacttt caccgacggt cggtcgactc
     4681 actgcgaccg ttccacgatg taagtggcgt gcagcagttc gaggaacgcc gccagggggg
     4741 gcgagacata ccggtctttg tggactacgg tgtagaacgg cctggtcaac tccacccctg
     4801 agacgtggag cgaaaccagc agcccggtcc tcaactccag ttccagcgtc gccctggaga
     4861 caatcgcgac tccaagaccg gcccgaaccg cccgcttcag cgcctccgga ctccccagtt
     4921 ccaacaccgg tttcagctcg atcccatact cggccagcct gccgtgaact atctctcgcg
     4981 ttccggaggt gggttccctt tccagaaagg gctcttcctg caactcctcg acagctaccg
     5041 acgacctccc cacccaccga tgctcgggcg ggactatcaa cgcaagctga tcgatgaaga
     5101 acggctcgac caccaattct ggggagccga ccggcccctc caccaggccg agatccatct
     5161 tccccgagac gatctgctcc acctgcctcg gcgtcggggc tatgtcgaca agaaggcgaa
     5221 cgccctcgtg caacttgtgg aaccgcccga acagctcggg cagcaggtag cttccgatgg
     5281 taaggcttgc ccccaccagg agttcgccgg cagccaatcc tctgaaggag gccatggtct
     5341 tgcgcgactc atcgacctgg gagaggatgc gaccagcgta gcccagcatc gactcccctg
     5401 catgggagag gacaaccctc ctccccatcc gatggaacag cttcaccccc aactccctct
     5461 ccagcgtcgc cacctgcata ctcacggtag actgggtcag gaagagggca tccgcggcac
     5521 ggctgaagcc tccctcctgc gccacggcac ggaacgacct caactgctcc agattcatcc
     5581 catctaccca tcgacagatt tgatactaat cagcagttat atctgcttga tcgatcatac
     5641 accctggagt accatcgcgg ctagggggta gattcgggag gcaaccgggt acgaagtcgg
     5701 catcaaccgg ctgcccgtcc aggcccgccg cccaacggta gtctcctaga gaggatcgtc
     5761 tggaatggac ccaacacagc tattcgcggc actcttcgca tcctccgtag cggcgggcgt
     5821 actggggtcg ctgctcgggc tggggggcgg gatcatcgtc atcccgatcc tcacgctcct
     5881 tctcggcgta gacatccgct acgccgtcgg ggcaagcata gtgtccgtca tcgcaacgtc
     5941 caatggggcg accgcttcgc acctcaggga tgggatagcc aacttgagag taggaatgct
     6001 gctcgagact gccacagcca ccggagcgat gatcggagcg ctggcgaccg cgatgatagg
     6061 cggacgcccc ttatacctca tcttcgcggt cgtgctgggg gtatccagcg tcgccatgtt
     6121 ctggaggcga aatgccgacg actgtcccaa tccggtcagt cacccctggg ccgaaaggct
     6181 cagactgggg agcagctttc cggaccgatc aaccggccgc accatacggt atggggttgc
     6241 aggggttcct gttgccatgg ggctgatgct gatggccggc gtggtctcgg ggctgctggg
     6301 agtggggagc gggaccctca aagtaccggc gatggacctg gccatgcggc tgccggtgaa
     6361 agtgtccacc gccaccagca atttcatgat cggggtcacg gccgcggccg gcgccgcggt
     6421 gcatttcact cgcggcgacg tccaacccct gctggccgcc cccgtggcgc ttggggtgct
     6481 ggtcggcgcc gtcgttggcg cacgcaccct gggcaggctt cacagcagtt cggtgcggac
     6541 cctgttcgtc ctcgttctgg tagtagtgtc ggtccagatg gcggcccgag gactgggatt
     6601 ccagatctga gaaagaggta tcatgatggc ctacgcgaag ggtcgcgact cggacaacag
     6661 agcgactgca gaagcgcgac tggttcccac cgaagtgacc atacatggag acagcacggc
     6721 cccatcgtcg atagcgaaaa acgggaggga caagcggctc gagggagtag tcgggccggt
     6781 gctccgaatc ggctccctca tcagcatggc gctgatcctc acgggattgg cgctcaccgt
     6841 actcggatgg ccggtcacaa gactgattgc gtctccagca cctaccctgg gaagcgtgtt
     6901 ccaaggacct gggggcacct tccccacaac cctgatcaac ctcgggatac tggtgctgat
     6961 cgccactccg gtcgcccgtg tggcctcgac tgtcgtcttc ttcctggcca ggggggaact
     7021 gtcctacctg gctatcaccg cctacgtgtt gatggtgctg ctggccggtt ttctgcttgg
     7081 cgcaaccggc taggggcgcg cttccaccac caacttgctc tgcaaggtct cgggcatcgt
     7141 caggagcaat ggaagacgca caaccaggtc gacggccaca aaccccaaga acacatattg
     7201 cgggccgact ctgtcccaca tgatgcccgc cacacctgcc atcaacgcac tgagaagcat
     7261 ccggaagaag cgagtgatcc ccagccagcg ccccatctga tcggctgtca ccaactccct
     7321 ctccatggcc gcgccgatgg gccccgatat gtagtagaag ccctggagga tgccggctac
     7381 gatcaggaac gccgaatcgg gagcccacac gaggacgagg ttcgagaccc aaaacagagg
     7441 cgttactgcg agcaggattg ccttcctgcc ccatcgatcg gcaagccggc ccaggggaat
     7501 ggcgagaacg atggatgtga cggcgcatcc ggtgaccatc acacccagca catacgcatc
     7561 agcacctttc gttgaatgcg cataaacctg agcgaagggg aagaccattg ccaacggtag
     7621 ttggccgacg gaggtgatta ccagccatcg cttcaggtgc cggccgtcct tcagcacctg
     7681 atgcagatcc ttgaacatac ggccgggtgc gccgcggaca ttcacccacc ggcggtcaga
     7741 tagctgggtc tgcacgagaa cgaagcttcc cagggtcacg atggtgctca cgacgaatag
     7801 agggcgaatg ccgtccaccg ctaccccgcc ggagccggct accagccagg ccccgatcag
     7861 gggaccggcc atcccgagca atccagcccc gactgtctcg cagatcatca tcccagtggc
     7921 gcggtcgcgg gtcgccagac agttcccgca aacggtggca cagccgagga cgctggtcga
     7981 gtatcccagc cagtatgcta ccatcgcaac cagagccatc tgccagttct gggctaggga
     8041 gtaggtcagg taggaaaagg ccagcatgac gatgccggcc aggtagaccc tcttcgcacc
     8101 gatcctatct atcaaccagc cggtggaagg gcccagggcg gcagccgcca ccattcccgc
     8161 gctgtttatg ccccccagtt gggtcgcggt ggctcccagg gcaacgatgt agatggacag
     8221 gtaggggaag accatctggt acacgagcct gtccagcgac gtccgggcga ccgtaacccg
     8281 ccagtcgcgc gactgcctgc ctacgaacga gatcccgctc gctgccgcct gaacagcgat
     8341 ggaggcgaat tgtgtcacta gaccgctccc ggtgggcaat atcacgtggg gaaacctgcg
     8401 ctcgtcactc aactgccctg gatgggtcaa gccggcttgt gggtctcgta cagggctttc
     8461 ttgaaatagc cctcgctgcc gtacagggca ccgattggat tatgagcgag caccctatct
     8521 ttggcaacaa gacacgtcac tggggccttg gaatgcatga tgaacaggga atcgtggccc
     8581 acgcataggc cgaatacgat gttgaaatcc gtcttctccc tgttgagcac cctggcctgg
     8641 ccgatgggat tgcacatcgc ctcgaactct ccggggtgaa ccttctgaca gtcggcgatg
     8701 tccaactcct ccttggggat cgcgccggtc ttgcacacca ccgaggcaac cttgaagccg
     8761 ttcgccttca gcacgcgcga caatacctta gcctcttccc gcaatcccac gcagaaggcg
     8821 attccaagct tctcgtaccc catccggcga gcgaactcca tggtctcctc gacccttgtc
     8881 cagcgcaggt agccctcgga ctccaccaac gccgactcgt gcgccatccg ccgcagggtt
     8941 gtgtctgaaa gagactcgca ggtgttggtc acatccccat cgcgcatggg gcaattcacc
     9001 ggcgccacct cagcgtggcc tattcggcat acgtatacct tgcactcagc acactcagcc
     9061 aaccgtaacg ggcccccttt ccatcttctg gctgctcaaa tcgagcagcc gggtaccaag
     9121 tgaagactca tccttgggtc gatccagccg ttgtaccctc agtcagcacc cccaaaaggc
     9181 tgccgccaga gaatctatac tctcggatgc atactcttcc aaacggcctg catcgcacaa
     9241 cggggcaaca gccggatcca gcggcagtct ggccagcagc ggcgccccgg ctgcccgagc
     9301 cagttcctcc ccacggctgg ggccgaagat ctcgtggtgg tggccggtgt cgggacagta
     9361 gacgggccag gctattccaa ccagtccatc cctatcattc gcccaatctt gacgtagccg
     9421 ggcgccaacg ggtactcgtg gagcatcgaa agagccagag gaagccccga ctcatactcc
     9481 ccgaaatgga tcagcccatc ccttgtctcc ggaagttgag cggcagggat cgagacggcg
     9541 atctcatcgt cgctggcaag cgctctcgcc ctatcgccgc gacatggaga ggccaccgac
     9601 cactcaccgg tctgaagggt tggcacgacc gcgtagatgc aggccgcgtg gccggagagc
     9661 ttgctggcaa cgtccacacc atccatccag tccttcacca tcagtagctg cgtcaactgg
     9721 gatggatcgc agtacactat caccagatcc ggctcgaaag tcgcctcctt cgccggagcg
     9781 gagacaaccc cgatatactc gccgagcgca agccggggca acgcctccac ccaggttcgc
     9841 cccacctcca gcgtccgagc ggccttcgga atctggtttt gcccctccag gaacagcttc
     9901 ggcggcttgg ccagtccgta ccccagtacc ggctcgaagc accacatgtc ctccttcgtc
     9961 tgagcgacag tcagcccatc tcggcgggac agggcaaaac cctggcacgt ggagaggtgg
    10021 cggcccaggt ccctgaccgg ccgcttcgcc ccttctggga tctcatcctc gctcctcagc
    10081 atcttcaccg ccaggggaaa gctcttcagg cgaagtatcg attcgatcgc cgccgcggca
    10141 tccctcagtg actgcagagt catcagcctt cctcccaacc ctatctctcg cgcggtctca
    10201 tggcagggca cccttccacc cgtctgtgat gaacagaggg aagaccgccc gaactagtgc
    10261 aaccgttccg tgtgattttc catggctccg ctgatgaacg cttggaccgc ctcgtcgatg
    10321 tcgacgatgt cggtgatgac cggctccaac ccgttggctt tgaggctggc gtaggccggc
    10381 ctgcccatgc cccctgccag cactatagtg caatccttga tggcagccgc catagcgttg
    10441 tggtcgtgac catggccgtg gatgtgctgg tgaagctcac catcaccctg cgggtggact
    10501 attcgaagct gagaagacct gtccctgact tcccgctcga ctactctgcc accctccacg
    10561 gtcaggacca cgtactgctt ggcccgtcca aagtgagcgc agatggtctt gccatcatca
    10621 gatactgcgg cgatcttcat tctgtctcct ttagccaatg cctctctgat ctgtgccctg
    10681 ctggtggatg ccatagcata cagggggtcc acccgccccg actccgactc attagccgcg
    10741 gccttgtggc aatgggggca agtgacggat gcccgcgacg acatcacctg ctcccaaccg
    10801 tggccacaac ccgggcagtg aaacctgctg ggaacgacct ggtaatcccc gccctcgatg
    10861 cgcaacgcct tgccatccac cagagcctcg gccaccttga agtgagcctg atccagcacc
    10921 ctctggaagg tcgggcgaga cacgcccatc ctgcgggcgg actcctcctg gtccaactcc
    10981 tccaggtcct tcagccgcag ggcttccaat tcatccaggg agagttgaac ctccttaggc
    11041 tcccgcatcg gggtgccctg gggcttgaag tatcgaacgt cggggagcaa gcccacacat
    11101 cttggtttcc tgggtctgct cactgtgatt ttccctccag cgccactgca acgaacagcc
    11161 aacaggctgc gcatgttgag agccgaagcc gcgccaaagc gggcattcgg ccctcaactt
    11221 attggtcata tgctcattag gatcttacgg caggatcgag ccgccgtcaa cggtccatcg
    11281 acacatcccc ctacgcccgc tctcgtctca cttcttcgtc ttcctggaat ccagccactc
    11341 ccgcagcttg ctcacggaga agtcctcgct ctccaccttg ttcatgacct ggtcggcagt
    11401 caccttctcc ctgggcagct tgaagttgtc ggcaatctcc tccaggctca cgttgtaccc
    11461 cctggagatc tcgccgaagg tcatccatcc ctttatctcc gctgtatccc ggccggtggc
    11521 cgtgatcggc gtacccgatc cgtcgaactt gccgctagtc gaccagtgtc cggtcgcccg
    11581 agccactcca tatggaagaa agaacaccac cagg
//
LOCUS       DATALS010000023         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_137211, whole genome shotgun sequence.
ACCESSION   DATALS010000023 DATALS010000000
VERSION     DATALS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137211"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VHS06_00510"
     CDS             <1..1033
                     /locus_tag="VHS06_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2986647.1"
                     /translation="LEAARVAANLGLSASLCAAAAQAGEPLVVEDTTRYARGSRSVLR
                     TENMRCMVRVPFKSSSGRLLGTMCLGTSASRSFSKAEVDLLVSVGNQVAAAVQNARLY
                     EEIRRKGRMRGELLEKVIAAQEAERKRIARELHDQTSQALAALAVAVETAAAQAEAGE
                     NVSGSLAKMKVLAVDTLEEVHRLIFDLRPTLLDDLGLIAALRWYVETRLGEVGIKVRL
                     EVGGEERRLAPQIETALYRVVQEAVNNVVNHSGAQNCTITLVLRADSLSLTMVDDGWG
                     FDMAELARSTDPKRGLGLMGMRERVELLGGTFAVYSELGNGTRITVEVPLREGEDQVD
                     GEDPSTAGG"
     gene            999..1652
                     /locus_tag="VHS06_00515"
     CDS             999..1652
                     /locus_tag="VHS06_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2986648.1"
                     /translation="MTERIRVLLVDDHAILRDGIKALIGLSRDVEVVGEADDGQAAID
                     RVAELLPDVVLMDIAMPRMDGLESTRRIKERHRNVRVLILTQHENREYVFPILKAGAD
                     GYVLKKAAGTELLSAIRAVHSGGTFLYPSVARAVVEDYLCPDGSVADRRHSKLSDREI
                     EVLKLVAEGRSNQEIADLLCLSVKTVTGHRTNIMEKLDLHSRTELVKYAIRTGLIQV"
BASE COUNT          423 a          586 c          724 g          376 t
ORIGIN      
        1 gctggaggct gctcgcgtgg ccgccaatct cggcttgagc gcgagcctgt gcgcggcggc
       61 cgcacaggcc ggcgaaccgc tggtggtaga ggacaccacg cggtacgctc gagggtcgcg
      121 ctcggtgctc cgtaccgaga acatgcgatg catggtgcgg gttcccttca agtcctcctc
      181 cggccgcctg ctgggcacca tgtgcctcgg caccagcgcg tctcgcagct tctccaaagc
      241 cgaggtagat ttgctggtct cggtcggcaa ccaggtcgcc gcggcggtgc agaacgcccg
      301 actgtacgag gagatccggc ggaaggggcg gatgaggggc gagttgctgg agaaggtgat
      361 tgcggcacag gaagctgagc gcaagcgcat agcccgggag ttgcacgacc aaaccagcca
      421 ggcgctcgcg gcgctggccg tggcggtcga gacggcggca gcgcaggcgg aggccggaga
      481 gaacgtgagc ggcagcctgg cgaaaatgaa ggtgctggcg gtggataccc tcgaagaagt
      541 acaccggctg atcttcgacc ttcggccaac gctgctggat gatctgggac tgatcgcggc
      601 gcttcgatgg tatgtagaga cccggctggg cgaagtcggc ataaaggtgc gtttggaggt
      661 ggggggcgag gagcggaggc tggctcctca gatcgagacg gcgctgtacc gcgtcgtgca
      721 ggaggccgtc aacaacgtcg tgaaccactc gggtgcgcag aactgcacga taacactggt
      781 cttgagggca gattccctat ccttgaccat ggtggacgat ggctgggggt tcgatatggc
      841 cgagcttgcc cgtagcaccg accccaagcg gggactgggg ctcatgggga tgagggagcg
      901 ggtggagctg ttgggtggca ccttcgccgt ctactctgaa ctgggaaacg ggacccgaat
      961 aacggtggag gtacccctgc gagagggaga agaccaggtt gacggagagg atccgagtac
     1021 tgctggtgga tgaccatgca attctgcggg acggcatcaa ggcgctgatt gggctctcca
     1081 gggacgtcga ggtcgtcggg gaggcagatg acgggcaggc tgctatcgat cgagttgccg
     1141 aactccttcc cgacgtagtg ttgatggaca tcgccatgcc ccgtatggac ggactggagt
     1201 cgacccggcg catcaaggag cgccaccgga acgtccgtgt gctgatcctg actcagcacg
     1261 aaaaccgaga gtacgtgttt ccaatcctga aggccggtgc cgacggatac gtcctgaaga
     1321 aggccgcggg gaccgagcta ctctcggcca ttcgagcggt tcacagcggt ggcaccttcc
     1381 tttatccgtc ggtggcgagg gcagtagtgg aagactacct ttgcccggat gggagcgtgg
     1441 cagaccgccg ccactccaag ctctccgatc gagaaatcga agtcctcaag ctggtcgccg
     1501 aggggcggag caaccaggag attgcggacc tcctctgcct gagcgtcaag accgtaacgg
     1561 ggcatcgcac caacataatg gagaagctgg acctccattc ccgcaccgag ttggtgaagt
     1621 atgcgatccg aacgggactc atccaggtgt gatctccaac acctgcgaaa tcgacatcga
     1681 ccgggatagg gcgttttccc tacccgttaa cagggcgttc acaggatgcc tcgtccctcc
     1741 ctactcccct agactccaga caggtggcac ctcgtctttc tgcctgcatg cctatggcgc
     1801 cttcaccagc gtcgtggtgg tcaacttcat cgtgggtctc atcatggcga tcttggtggc
     1861 tttccctgga tgcagtcaac gtctccctta ggggagagtg gataccaaca agggaaagtc
     1921 gcttagttct tcggcgggga gattgcgaag ccgaaggact gacgcgatcg ctgggaccat
     1981 tgggcgtacc aacggtggta acgattagga ggtagcttca tggcagaaga gtccaagaca
     2041 ccgatgctgg accaactcga agggggatcc tggccgagct tcgccaaaga gatcaagcgg
     2101 cgggcgaag
//
LOCUS       DATALS010000024         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_316434, whole genome shotgun sequence.
ACCESSION   DATALS010000024 DATALS010000000
VERSION     DATALS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316434"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VHS06_00520"
     CDS             <1..874
                     /locus_tag="VHS06_00520"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX2986649.1"
                     /translation="VDWATMTSMAMVVLATVVALRLLRMAVRGIARGVLQRDRELERE
                     VAQKAKTLSQVAETTGRIVIFTLAGLTILTLSGRDITPLLASAGIAGVAVGFGAQNLI
                     KDWLSGFFILLENQYSVDDVIKVGEYSGTVERLDLRRTVLRNLEGSVIVIPNGEVRTV
                     TNLTKEWSRVVMDVGIPYEEDEDRVIEGLRRVGADLAEDVEIGGMILEPPEVLGIELL
                     GQYQVTIRMLVKTLPTKQWLVARALRRRVKRMFTQEGFQFPYPHQIAINQFPPGGAMD
                     GTLATVVRQKERSE"
     gene            complement(853..1737)
                     /locus_tag="VHS06_00525"
     CDS             complement(853..1737)
                     /locus_tag="VHS06_00525"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX2986650.1"
                     /translation="MGQENREFIDGGWGMRSLLIVNADDFGISEGVNRGIAEGHQKGI
                     LTSTTLMANMPAFDHARQVSAENPGLAVGVHLTLTGGRPVLPPQKVPHLIDVNGRFLR
                     ADRFLIRVSTGGIPAGEIEAELCAQVERTLGGGFPLDHLDSHHHIHVHPAMQAIAIRI
                     ALRFGIRGIRCPVEISGKEMSAKPSVAKALVLGTLGNMLRLRARRVGLASPDHFRGVI
                     LGMAFSTGPLHSMLRRLPQGITELMCHPGYPDEGLRQQTVYAEGRERELEALLDPAGR
                     RILQQKGIALGDYSDLSF"
     gene            1820..>2388
                     /locus_tag="VHS06_00530"
     CDS             1820..>2388
                     /locus_tag="VHS06_00530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2986651.1"
                     /translation="MSKFDKRNVISQALCLFDDQPLGIRAFVRTRNMLCPMRNVEAAV
                     PQQGRILELGCGHGLFSAVMAMSAPERSILGVDPSSVKIDVASRMGQRLPNAEFFQGT
                     IDDVHEDGLAGIAIIDVLYLLPVEEKLRILRRCHELLAPNGRLVLKTNDTHPVWKYRW
                     AWFEEWLGYAIAVDRECRRLRHQRRGESG"
BASE COUNT          442 a          716 c          758 g          472 t
ORIGIN      
        1 agtcgattgg gcaaccatga cctcaatggc aatggtggtg ctggccaccg ttgtggcgtt
       61 gcggctgctg agaatggccg ttcgtggaat agctcgtggc gtcttgcagc gagatcgcga
      121 gttggagcgg gaggtagccc agaaggcaaa gacgctctct caggttgcgg agaccaccgg
      181 caggatcgtg atcttcaccc tggccggtct cacgatcctg accttgagcg gccgggatat
      241 aacacccctc ctggcgagcg ccggtattgc cggtgtcgcc gtcggcttcg gtgcacaaaa
      301 cctgatcaag gactggttga gcggcttctt tatcctgctg gaaaaccagt actcggtcga
      361 tgacgtgatc aaggttggcg agtactcggg cacggtggag cgactggatc tgcgcagaac
      421 cgtgttgcgc aacctggaag ggtcggtgat cgtcattccc aacggcgagg tgcggaccgt
      481 caccaacctg acaaaggaat ggtccagggt ggtcatggac gtgggcattc cctacgagga
      541 ggacgaggat cgtgtgatag aggggttgcg ccgcgtaggg gctgatctgg ccgaggatgt
      601 ggagatcggc ggaatgatcc tggagccgcc cgaagtgctc gggatcgagc tgttagggca
      661 ataccaggtg actatccgga tgttggtcaa gactctgccc accaagcagt ggctggtggc
      721 tcgtgcgctg cgaaggcggg tcaagaggat gttcacccag gaggggttcc agttccccta
      781 cccccaccag attgcgatca accagttccc gcctggaggg gcaatggacg gcacactggc
      841 aacggtggtt cgtcagaagg agagatccga gtagtctccc agcgctatcc ccttctgctg
      901 caggatccgc cggccggccg ggtccagcag cgcctccagc tccctctccc ttccttcggc
      961 atagacggtc tgctgcctga gcccttcatc ggggtagccg gggtggcaca tcagttcggt
     1021 gatcccctgg gggagtctgc gcagcatgga atgcagcggg cctgtcgaga aggccattcc
     1081 caggatcacg ccgcggaaat ggtccggcga tgccagcccc accctgcggg cgcgaagtcg
     1141 cagcatgttc cctagggtgc ccaacactaa cgcctttgcc acggagggtt tcgcactcat
     1201 ctccttgccg ctgatctcca cgggacagcg aataccccgg atgccgaaac gaagggcgat
     1261 cctgatggca attgcctgca tggcggggtg gacgtgaatg tggtgatggg aatccaggtg
     1321 gtccagcggg aaccctcccc ccagcgttcg ctctacctgc gcgcaaagct ccgcctctat
     1381 ctccccggct gggatcccgc cggtgctcac ccggatcagg aaacgatctg cccggagaaa
     1441 tcgtccattg acgtcgatca gatgggggac cttctgtggt ggcagtaccg gcctgccgcc
     1501 tgtaagggtc agatggacac ctacggccag gcccgggttc tcggcactca cctgcctggc
     1561 atggtcgaag gcgggcatgt tggccatgag ggttgtactg gtgagtatgc ccttctggtg
     1621 gccctccgcg atcccccgat tcaccccttc gctgatgccg aagtcgtcgg cattcacgat
     1681 caacagcgat cgcatacccc agcccccatc gatgaactcc cgattctctt ggcccatccc
     1741 cgacccctat cccccatccc ccgccgcatt gtatactcac gccacgctat gcagctacag
     1801 caagacgagg aagtccaagt tgtccaagtt cgacaagcgt aacgtcatct cccaggccct
     1861 ttgcctgttc gacgatcagc ctctcggcat tcgagcattt gtcaggacgc gcaatatgct
     1921 gtgcccgatg cgcaacgtgg aagcagccgt gccgcagcag ggacgcatcc tggagttggg
     1981 ctgcgggcac ggtttgttct ctgcggtaat ggccatgtcg gcgcccgagc gctccatcct
     2041 gggtgtcgac ccctccagcg tgaagatcga cgtcgcgtct cgcatggggc agcgtctccc
     2101 taacgcggag ttcttccagg gaaccatcga cgacgttcat gaagacggcc tggccgggat
     2161 cgccatcatc gacgtcctct atctgctccc tgtcgaggag aagctgcgga tcctccggcg
     2221 ttgccacgaa ctgctggccc cgaatgggcg gctggtcttg aagacgaacg acactcaccc
     2281 agtgtggaag tatcggtggg cgtggttcga ggagtggctg gggtatgcga tcgctgttga
     2341 tcgtgaatgc cgacgacttc ggcatcagcg aaggggtgaa tcggggga
//
LOCUS       DATALS010000025         5086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_137399, whole genome shotgun sequence.
ACCESSION   DATALS010000025 DATALS010000000
VERSION     DATALS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137399"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..910)
                     /locus_tag="VHS06_00535"
     CDS             complement(224..910)
                     /locus_tag="VHS06_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2986652.1"
                     /translation="MSPMKALLVDDHALFLEGLQNLLLARGLDVVGTANDGLEALARA
                     RELHPDVVLMDIQMPRCNGLEATRLIKAEMPDVKIVMLTMSAEDEDLFQAIKAGASGY
                     LLKNLDAGDFFDLLSGLEHGEAPLSKGLAARILDEFARQAGKSPSPSRGLGDEPLDEL
                     TPRQMEVLTLVAQGMTYKEIGATLCLTERTVKYHMGQILEKLHVKKRAQAIAYARNSH
                     MISGNRSASE"
     gene            complement(907..2616)
                     /locus_tag="VHS06_00540"
     CDS             complement(907..2616)
                     /locus_tag="VHS06_00540"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEX2986653.1"
                     /translation="MNWQAALYVLALLSAAATTDLLALYAWRRRTVPCATPFFLTMLL
                     VTLCCLASALETIVEGLPSKLFWANVQYFSYAFLPVTMIMGALEYTGRDHLLTRRRLV
                     LLCVIPAITLTLLWTNDWHHLVRAAPFIESNGYISVVGKSFGPWYLVHAIFCYGLFGV
                     TMLILADAAFHAPPHHRGQPIGLLAGSIPPVFASLSYSLGTGPARFDQTQVAFAVMGL
                     ILAWSIFRHRTFNLVPLAHHKIVEGMNDGVVVLDAAGQVVEMNPAASRILGLSLKECL
                     GRPGKEVLDSWPVLVKLSGLAVDARVGMELATDDSVSHFDVQVSMSLDRNRRIIGKVI
                     ALRDLTEQQKSQQRLVEQQKSLAVFEERDRLARELHDSLGQVLGYVNTQAQAVRTLLV
                     QGDTARADSHLARLVAVTQDAHADVREFILNMKSPARLAGGLVPALESSVRRFQQLYG
                     ITAQLTLPERLEDDLISPEAEVQLLRIVQEALANVRKHARARRALVTLSVQADHLLTV
                     VEDDGCGFDPARLPDHLDHSFGMRIMNERASEVGGSLSVQGIPGMGTKVIVQIPFRKE
                     TSE"
     gene            complement(2767..4356)
                     /locus_tag="VHS06_00545"
     CDS             complement(2767..4356)
                     /locus_tag="VHS06_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX2986654.1"
                     /translation="MAGRVVLDADGHEPSRLTPAFATKLWASLHPLVQWEKHFWLTTA
                     MVVVGTFLHYFCPIPVGSANGPLQFSSQSMHRILFMVPVAYAGFIFGPRGGFLTLFVV
                     SLVMVPKAFFMSADLSHDVVETLCTVLVSFLIVLWFNGQQREKSRRFEILSRLELARQ
                     ELAFQVDVIKKNEKRLAVLHEEAVASEQKYRDLFENATVAILVQDLQGTITAANNACT
                     PLTGYLPGVLIGTSVRRLFPSSAYSVLSSVQQAMLNGDDSVQLYDQPYDLQMVRRDGS
                     ESILSVTTRLVGENGTPRGFQHIAIDVTERRRMRDVMNYYVRQVLTAQEEERNRIARE
                     LHDDTAQSLFLLLQKIEGMTYGAQKKLPNPARRELEEVRSVALQTLGNLRRLTRDLRP
                     QILDDLGLVAALEWLAEDMERQSGIRTKVEITGPQRPLAPEAQLLLFRITQEALSNVR
                     RHSDASTVAAVLEFVDGKVRVSVEDDGHGFTVPSSLSDLAGKGKLGILGMSERARLLG
                     GEITVESEPGRGTRVTAELVA"
     gene            complement(4505..>5086)
                     /locus_tag="VHS06_00550"
     CDS             complement(4505..>5086)
                     /locus_tag="VHS06_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2986655.1"
                     /translation="GDLEVVGEAGDGEEAVRLAEELSPDVALMDIVMPKLSGIEATRR
                     IKSTRPGTAVLILTAFEDDRYILGLLEAGAAGYLLKSSRAGDLVGAIRAVAAGESVLL
                     PAVTARLLARAASSGEGFAWSRNGEQLTERELEILRLAARGMGNKEIAKELALSVPTV
                     KAHLVNIFNKMGVGSRTEAVLQAVRKGWVGLEP"
BASE COUNT          990 a         1652 c         1547 g          897 t
ORIGIN      
        1 atgtccggca gctatacgaa ctactagagg acaaacggag acgccgaccc actcgaggta
       61 gtcgtaatat tgtggatgca ccgaagatag tcagcaacgg ccaatagtat aggaaaaggt
      121 acaggaaaag tacagctttc ctcatgataa cattggccga gacttctcaa gggccacacg
      181 gagaggcgcc tttggccccg ccaaacaggt gccggttcca ccactactcc gaggcggatc
      241 tgttgcccga gatcatgtga ctgttccggg cataggcgat cgcctgtgcc cgcttcttga
      301 cgtgcagctt ctctagaatt tgacccatgt ggtacttgac ggtgcgctct gtcagacaga
      361 gggtggcgcc aatctccttg taggtcatcc cctgggcgac gagggtcaac acctccatct
      421 gccgcggcgt cagttcgtcc agcggctcat cccctaagcc gcgggaaggc gagggggact
      481 tacccgcctg cctggcgaac tcgtcgagga tgcgggccgc caatcctttg gaaagcggcg
      541 cttccccatg ctccagcccc gaaagcaggt cgaagaagtc gccggcgtcc agattcttca
      601 gcaggtagcc cgaagcgccg gccttgatgg cctggaagag gtcctcgtcc tccgcggaca
      661 tggtgagcat cacgattttg acgtcgggca tttccgcctt gatcagccgg gtggcctcca
      721 ggccgttgca gcgcggcatc tggatgtcca tcagtacgac gtccgggtgc agttccctgg
      781 cgcgagccag ggcctccaat ccatcgttgg ccgtaccgac tacgtccagg ccacgcgcca
      841 gaagcaaatt ctgcaacccc tcgagaaaga gggcgtgatc gtcgaccagc agcgccttca
      901 tggggctcat tctgatgtct cctttctgaa agggatctgc acgatgacct tcgtgcccat
      961 gccggggatt ccttgaaccg agaggctccc gcccacctcc gacgcccgct cgttcatgat
     1021 gcgcatgccg aagctgtgat cgaggtggtc gggcagtcgg gccggatcga agccgcatcc
     1081 atcgtcctcg accacagtca gcaggtggtc cgcctgaacc gagagagtga caagcgctcg
     1141 gcgtgcacgg gcgtgctttc tgacgttcgc cagcgcctcc tggacaatcc tcagcaactg
     1201 gacttcagcc tcggggctga tgaggtcatc ctccagcctt tcaggcaggg tgagctgcgc
     1261 agtaatgccg tatagctgct gaaaacgtcg aacggacgac tccagggccg ggacgagccc
     1321 gccggcaagg cgagccgggg acttcatgtt caggatgaac tccctcacgt cggcgtgggc
     1381 atcctgcgtc accgccacca gcctcgccag atggctgtcc gccctggctg tgtccccctg
     1441 gacgagcagc gtacggaccg cctgcgcctg ggtgtttacg taccccaaca cctggcccag
     1501 gctgtcgtga agctcccggg caagccgatc gcgctcctcg aaaacggcca gcgacttctg
     1561 ctgttccacc agcctctgct gggacttctg ctgctccgtc aggtcccgaa gcgcgataac
     1621 ctttccgatg attcggcggt ttcgatccaa agacatcgag acctgcacgt cgaagtgact
     1681 cacggagtca tcggtggcta gctccatccc cacacgggcg tccaccgcca gcccgctcag
     1741 cttgaccagg accggccaac tgtccagcac ctctttgccg ggtctgccca ggcactcctt
     1801 gagggagagt ccgaggatcc gcgaggcggc cggattcatc tccaccacct ggccggccgc
     1861 gtccagcacc acaaccccgt cgttcatgcc ctccacgatc ttgtggtggg caagcgggac
     1921 gaggttgaag gttcggtgcc gaaagatgct ccaagccagg atcaatccca tcactgcaaa
     1981 ggctacctgc gtctggtcga agcgcgcagg gccggttcca aggctgtagc tcaagctggc
     2041 gaagaccggc ggaatgctgc cggccagcag gccgatgggc tggccgcgat ggtggggggg
     2101 cgcatggaac gcggcatcgg ccaggatcag catcgttacc ccgaagagac cgtagcagaa
     2161 gatggcgtgc accaggtacc agggtccaaa gctcttaccc accacggaga tgtatccgtt
     2221 ggactcgatg aagggggcgg cgcgcaccag gtgatgccaa tcgttggtcc acagcagcgt
     2281 caaggtgatc gccggaatga cgcacagaag gacgagccgc cggcgggtca acaggtggtc
     2341 gcgcccggtg tactccagcg ctcccatgat catcgtgacc ggaaggaagg cataggagaa
     2401 gtactgaaca ttcgcccaga acagcttgga ggggagtccc tccacgatcg tctccagggc
     2461 agaggccagg cagcacaggg tgaccagcag catggtgagg aagaaggggg tggcacacgg
     2521 cacagtgcgg cgacgccacg cgtacaacgc caggagatcc gtcgtggcag ccgcggaaag
     2581 cagcgccagg acgtagaggg cagcctgcca gttcatgctt tccaaccgaa tcaataaagg
     2641 ggcggcacgg aggccggccg aagctgccgg cccatccctg ggatcgccac cgtgagacag
     2701 gggtggggca caccgtcatg gggtggagcg aaaccgagga ttaagggaat ggtacaccaa
     2761 caaaactcaa gctaccagtt ctgccgtcac tctcgtgcct cttccgggct ccgattccac
     2821 cgtgatctcc ccgcccaaca gccgggcccg ctccgacatt cccaggatcc ccagcttccc
     2881 cttaccggcc agatcgctca ggctgctggg cacggtgaat ccgtggccgt catcctccac
     2941 cgaaaccctg accttgccgt ccacgaactc gagcaccgcc gccaccgtcg aagcgtcgga
     3001 gtgccggcga acattgctca gggcctcctg ggttatgcgg aacagcagca gctgcgcctc
     3061 cggcgccagc ggccgctggg gccccgtgat ctcgaccttc gtccggatgc cgctctgccg
     3121 ctccatatcc tccgcgagcc attccagtgc ggcgacgagc ccaaggtcgt ccaggatctg
     3181 ggggcgcagg tcgcgcgtga gccggcgcaa gttgcccagg gtttggaggg ccaccgaccg
     3241 cacctcctcc aactccctgc gggccgggtt cggaagcttc ttctgggcgc cgtaggtcat
     3301 cccctcgatc ttctgaagca gcaggaagag ggactgggcg gtgtcgtcgt gaagctccct
     3361 ggcgatccga ttccgctcct cctcctgggc ggtgagcacc tggcgcacat agtagttcat
     3421 cacatcgcgc atccggcgcc gctccgtcac gtcgatggcg atgtgctgaa aacctcgggg
     3481 cgtgccattc tcgcccacca gacgggtggt caccgacagg atcgactcgg agccatctcg
     3541 ccgcaccatc tggagatcgt acggctgatc gtacagttgt acggaatcgt ccccattcag
     3601 catcgcctgc tggacggagc tcagcacgga gtaggcactg gacgggaaca gccgccgcac
     3661 gctcgtgccg atcagcacgc cgggcaggta gccggtcaag ggcgtgcagg cgttgttggc
     3721 cgccgtgatg gtcccctgca ggtcctggac gaggatggca accgtcgcgt tctcgaagag
     3781 atcccggtac ttctgctccg aggccactgc ttcctcgtgc agcacggcca gccgcttctc
     3841 gttcttcttg ataacgtcga cctggaaggc caactcctgc ctggccaact ccagcctgct
     3901 gaggatctcg aaccggcgcg acttctcccg ttgctggccg ttgaaccaca ggacgatgag
     3961 gaacgagacg aggactgtgc agagtgtctc cacgacgtcg tgagagagat ccgcggacat
     4021 gaagaacgcc ttggggacca taaccagact gacgacgaac agagtgagga aaccgccgcg
     4081 agggccgaag atgaaccccg cgtaggccac cggaaccatg aataggatgc gatgcatact
     4141 ctggctgctg aactgcagag ggccgttggc actaccgacc ggaatggggc agaagtagtg
     4201 aaggaatgta ccaaccacaa ccatcgcggt ggtgagccag aagtgcttct cccactgtac
     4261 caggggatga agcgatgccc agagcttcgt cgcgaatgca ggggtcaacc tgctgggttc
     4321 gtggccgtcg gcgtcgagga caaccctacc tgccatggag ccctccgctg atgcagcaga
     4381 ttcacccgct ccactcttca gggcaggcag ccagtatcaa ttctcggacg gggagtgact
     4441 aggtcagagg gggggcggct gttccagcgg gggctccgct cctcagagcc ccaatacggt
     4501 gggtctacgg ctctagcccc acccatcctt tgcgaacagc ttgcagcacc gcctcagttc
     4561 gcgaaccaac tcccatcttg ttgaatatgt tgaccaggtg agccttgacc gtgggcacgc
     4621 tcagagctag ctccttcgct atctccttgt ttcccatgcc cctggcagcc aaccggagaa
     4681 tctccaactc ccgctcggtc agctgctctc cgtttcgtga ccacgcaaaa ccctcgccgg
     4741 aactcgccgc cctggccaga aggcgagccg tcaccgcggg caacagcact gattctccgg
     4801 cggctacggc gcggatggcg ccaaccagat cgccggccct ggagctcttc aggaggtagc
     4861 cggccgcgcc ggcctcgagc aagccgagga tgtaccgatc gtcctcgaag gcggtgagga
     4921 tcaacaccgc tgtgcctggc cgggtgctct tgatccgccg agtggcttca atgccactga
     4981 gcttgggcat cactatgtcc atcagagcga catccggtga aagctcctcg gccagcctga
     5041 cagcttcctc cccgtcgccg gcttccccca cgacctccag atcccc
//
LOCUS       DATALS010000026         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_137404, whole genome shotgun sequence.
ACCESSION   DATALS010000026 DATALS010000000
VERSION     DATALS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137404"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="VHS06_00555"
     CDS             complement(<1..1787)
                     /locus_tag="VHS06_00555"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX2986656.1"
                     /translation="MPESNAIKRQVMLIVVVALLFGAVELASSSVGFYTDWLWFSSVG
                     FQGIFLTMLEAKVLAFVLGAVAFMVPALISIMLARTIMAKHHTVSIREEQGVAYIVQL
                     GQDVPRRVVYGVAGLVAFGLSVMMGLSASGQWERFLKFANARGFGFADPLFSQDVGFY
                     FFTLPFFRFLQGWLFGSILLILLLTVVVYLVSIYEGTIGLDPGVFLANRAAKLHVMVL
                     AALAALVMAASYRIQLYDLVYSARSVSFGAGFTDASARAMALWALLGIMVIIAALFVV
                     SGFRHGFALPLWGVGLWVAAAILLGNVYPIVVQKLQVEPSELEKEKPYIESSIKMTRQ
                     AFGLDSIQEQDFKAEDGVSLDEIRANPDTVSNIRLWDHRPLKDTYNQIQAIRLYYDFN
                     DVDVDRYMLDGKYRQVMLSARELSPDKLASQAQTWLTRRLQFTHGYGMAMSPVNEIVG
                     EGLPNLLVKDLPPQGDVKIDKPQIYYGEVTSGYVIVKTTAQEFDYAKGDENVYGVYDG
                     DGGVRLDSFFKRIAYALYFRDGNILFTNYLTPDSRVLYVRDIQERVQKIAPLPDAGPG
                     SLSGGSRRPALLDPGCLHLRQRIPLLRPLS"
     gene            complement(2167..>3684)
                     /locus_tag="VHS06_00560"
     CDS             complement(2167..>3684)
                     /locus_tag="VHS06_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX2986657.1"
                     /translation="DPRTVNPLDLDTILASVQKTHRAIVIDEGYESYGVTAEIASRIA
                     DRAFDYLDAPVKRYGAMDIPVPFSPPAGVLHHSHVGASCGAGPRGDGEVTMAFEVVMP
                     QLGLTMTEGTVVQWIKKVGDQVKRGDALFEVETDKVTQEVPSVDEGLLSRIVVGEGKP
                     VPIGTVIAYLTAPGEAVPDAAAPAAAAAPAAAATAVAAPVEAPAAAAAVVVRASPAAK
                     KLAAKLGVDINQVQGSGPGGRIVEADIQKAAAAAATPVAVAPAATPTPISQPSTPVAG
                     ERIELKGARKVAAERMAMSFSTVPHFYLTVEVEAEALKAFREQIKGAVAKRFGVTPTI
                     TDLLIKALGMALTEQPDVNASYANGAIVRNPKVNLGVAASVDAGLVVPVIPGVEGLNL
                     GGIAQKRKALVERARVNQLALTDLEGGTFTLSNLGMFGIDQFQAIVNPPQAAILAVGR
                     IKERVGLVDGQVKARPTMFMTLSVDHRVLDGAAGAKFLARVVELLENPVELMLGE"
BASE COUNT          713 a         1217 c         1053 g          701 t
ORIGIN      
        1 gatagggggc ggagtagggg tattcgttgc cgtaggtgta ggcatcctgg atccagtaaa
       61 gctggccgtc ggctgccacc agatagggat cctggtccag catcaggaag gggggcaatc
      121 ttctgcaccc gctcctggat atctcgcacg tagagcaccc ggctatcggg ggtcaggtag
      181 ttggtgaaca ggatgttccc atcgcggaaa tagagggcgt aggcgattcg cttgaagaag
      241 gaatccagcc gaacccctcc gtcgccgtcg tagacgccgt agacgttctc gtcccccttg
      301 gcgtaatcga actcctgcgc cgtcgtcttg acgatcacgt agcctgaagt cacctcgccg
      361 tagtagatct gcggcttgtc tatcttcacg tcgccctggg gtggcaggtc cttcaccaac
      421 aggttgggaa gcccctcgcc cacgatctcg tttaccggac tcatggccat gccgtaaccg
      481 tgggtaaact ggagtctccg ggtgagccag gtctgcgcct gggaggccag cttgtcaggc
      541 gaaagctctc tggcggagag catcacctgc ctgtacttgc cgtccagcat gtagcgatcg
      601 acgtccacgt cgttgaagtc gtagtagagc ctgatcgcct ggatctgatt gtaggtatcc
      661 ttcagcggcc tgtgatccca cagccgaata ttgctcaccg tatcggggtt cgcgcggatc
      721 tcgtccaggc tgacgccgtc ctccgccttg aagtcctgct cctggatcga atccaacccg
      781 aatgcctgtc gggtcatctt gatgctggac tcgatgtagg gcttctcctt ctccagttcg
      841 ctgggctcca cctgaagctt ctgcaccacg atgggataga cgttgcccag caggatggcg
      901 gcggcaaccc acagccccac gccccatagt ggaagggcaa agccgtggcg gaagccgctg
      961 accacgaata acgccgcgat tatcaccatg atccccagca gtgcccatag ggccatggct
     1021 cgcgcgcttg catcggtgaa gccggcgccg aaggacacgc tccgggcgga gtagaccaga
     1081 tcgtaaagct ggattcggta ggaggcggcc atcaccagcg cagccaacgc cgccagcacc
     1141 atcacgtgga gtttggcagc ccggttggcg aggaataccc ctgggtccag gccgatggtg
     1201 ccttcgtaaa tgctcaccag gtacacgacg acggtaagca gcaggatcaa caggatcgag
     1261 ccaaatagcc acccttgcag gaaacggaag aagggcagag tgaagaagta gaagcccaca
     1321 tcctgactga atagcgggtc ggcgaaaccg aagccgcggg cattggcaaa cttcaggaaa
     1381 cgctcccact ggccggaagc cgagaggccc atcatcactg agaggccgaa ggcgacgagg
     1441 ccggccacgc catacaccac ccgccgaggg acatcctggc caagctggac gatgtaagcg
     1501 accccctgct cctcccggat ggagacggtg tggtgcttcg ccatgatcgt cctggccagc
     1561 atgatgctga tcagagccgg caccatgaat gccacggccc ccagcacaaa ggcgagcacc
     1621 ttggcctcca gcatggtgag gaagataccc tggaacccca cactgctgaa ccacaaccag
     1681 tccgtgtaga agccgaccga actggaagcg agctcgaccg ccccgaagag cagagcgacg
     1741 acgacgatga gcatgacctg ccgcttgatg gcgttacttt caggcaactg gagcctcctt
     1801 ctatgctatg aggagctggg atgggtgcgg catcgtaatc cgataattgc gggcagagca
     1861 taacataatc agggacgggg gtcacttacg ctggcggcac ccaggacagg aacatgaggg
     1921 cgttgacgca gtggagatga tacgtactgc cgtgttgacc gaagcgaagc atctcctcgt
     1981 gaagggaggg atccctcagt gccgctcagg atgacagctg tacagggcac gacactcgta
     2041 aggttcaaac aatcggacaa gcacattaat aaaggaaggg caccaggttg ttcttgccaa
     2101 cctggtgccc ttcgagactt agcgctattc gatattcagg gtctaccggc atcctgtctc
     2161 cgccggctac tccccgagca tcagctccac ggggttctcc agcaactcca ccacacgtgc
     2221 caggaacttc gcccccgcgg ctccgtccag cactcggtgg tccaccgaca gcgtcatgaa
     2281 catcgtcggc cgggccttca cctgaccgtc caccagccct actcgctcct tgatccgccc
     2341 tactgccaat atcgctgcct gcggcgggtt cactatcgcc tggaactggt cgatcccgaa
     2401 catacccagg ttcgatagcg tgaaggtccc tccttccaga tcggtcagcg ccagctggtt
     2461 caccctagcc ctctctacca gcgccttcct cttctgcgct atccctccca ggttcagccc
     2521 ctctactcct gggatcaccg gcaccaccag ccccgcatct acagacgccg ctacccccag
     2581 gttcaccttc gggttgcgta cgatcgctcc gttcgcatag gacgcgttca cgtccggctg
     2641 ctccgtcagc gccataccca gcgccttgat cagcaggtca gtgatcgtcg gtgtcacccc
     2701 aaaccgcttc gccaccgccc ccttgatctg ctccctgaac gccttcagcg cttctgcctc
     2761 cacctccacc gtcaggtaga agtgcggcac cgtcgagaat gacatcgcca tcctctccgc
     2821 cgccaccttc cgcgctccct tcagctcgat cctctcccct gccacggggg ttgagggttg
     2881 ggagatgggg gttggggttg cggcaggtgc caccgccacc ggagtcgccg ctgccgccgc
     2941 tgccttctgg atatcagcct cgactatccg tcctccgggg ccgctccctt gcacctggtt
     3001 gatatccact ccaagcttcg ccgccagctt cttggccgca ggcgatgccc ttaccaccac
     3061 cgctgctgcc gccgcaggcg cttccaccgg tgccgccact gcagtcgctg ctgccgctgg
     3121 tgctgccgct gctgcaggcg cagcagcatc ggggactgcc tcgccgggag ccgtcaggta
     3181 ggcgatcact gtgccgatgg ggacgggctt cccctcaccc accactatcc tgctcagcag
     3241 cccctcgtct accgatggga cctcctgcgt taccttgtcg gtctcaacct cgaagagcgc
     3301 atcgcccctc ttgacctggt cgccgacctt ctttatccac tggaccaccg tcccctcggt
     3361 catcgtcaga ccgagttgcg gcatcactac ctcgaaggcc atggttacct ccccatcacc
     3421 tcgcgggcca gcgccgcaag atgctccgac gtgggaatgg tgaagaactc cagcgggggg
     3481 gctaaacggg acagggatat ccatcgcgcc gtaccgcttt accggcgcat ccaggtagtc
     3541 gaacgcgcgg tcggcaatcc gcgaggcaat ctcggcagtg acgccgtagg actcgtatcc
     3601 ttcgtcgatc actatggccc gatgggtctt ctgcacagag gcgaggatcg tgtccagatc
     3661 cagcgggttc accgtcctgg ggtc
//
LOCUS       DATALS010000027         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_137731, whole genome shotgun sequence.
ACCESSION   DATALS010000027 DATALS010000000
VERSION     DATALS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137731"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VHS06_00565"
     CDS             <1..1017
                     /locus_tag="VHS06_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX2986658.1"
                     /translation="AFVAVTVAISFATLVVGELAPKNLAIMRAEPIALTLSGPVNFLS
                     RVAAPLVWVLTSTTGLVMKLVGGPDRVRISPITEEEIMAMVASGEEAGVVEPTERKLI
                     DEVFEFGDTITEEVMVPRVDVRALSRDTTVAEARKAIVETGHTRLPVYEGSLDNIIGV
                     IHAKDVLQELPPDAPDEQVNRAVTSIMRRAYHVPGSKRVAELLPELQRQQLHLAVVVD
                     EYGGTAGIVTLDNLLEEIVGPIRDEYDAREEPDIQMIGEEQAVVSGGADLGDVGSALE
                     VDLETEGVDTIGGLVYARLGRIPVEGDSVELPDALVEVISMRGRRVWKARVTRRELPP
                     EEEE"
     gene            complement(1028..2029)
                     /locus_tag="VHS06_00570"
     CDS             complement(1028..2029)
                     /locus_tag="VHS06_00570"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEX2986659.1"
                     /translation="MSFRESATASLIGEKALALLESPAFWGRVNAVVTNAVYLQGMGG
                     ELLWIGPTGHVAHRRCLQVPLGMRQVPGGTVFRNQGRRLLLGEGLSIDWAGAQLWRPE
                     RIRPDDAIPLPVLAANLVSILESVIPQCCSDGLAQAMPFLRALLPDTSRPSGNGTNGH
                     TASCSTTLVPRESSETNPLIPGKALGAVSAVADACIHHDGGAMVQAGRGLVGLGPGLT
                     PSGDDYLGAVLFALSQMDLAYPGLCRWRLTPSAELVDMARSQTNWISHTLMSDMAEGH
                     AAEPLHRFVLRVLRDPEPGGALDELPGLLEIGHSSGWDMLTGALTGMLASIDPGLGL"
     gene            complement(2284..2808)
                     /locus_tag="VHS06_00575"
     CDS             complement(2284..2808)
                     /locus_tag="VHS06_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986660.1"
                     /translation="MTQRGKGQQGMSAASQGGGGSHQTPHHPSGAQPHGKGRQPSTTH
                     ARMSDEQFLHKFGGQLSKTTQNSIWIRSPQEHEERPGESLGTRSHEVIQQWAEERGGQ
                     PATVPGTEHEGRPGVLRFNFPGYGGQGLQPISWEEWFRTFDERDLVFLFQEHKSNGDM
                     SNFFRLDSPHREEA"
     gene            complement(2878..>3303)
                     /locus_tag="VHS06_00580"
     CDS             complement(2878..>3303)
                     /locus_tag="VHS06_00580"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEX2986661.1"
                     /translation="VPYEVRSFEHDGFTPAKEAAERLTMSPDTLFKTLIVRGERNGVV
                     LALVPGDQTLSLRKLAQQMGDKRAEMVDPSEITRLTGFVKGGVSPLGSRRAYPVFMDQ
                     TALKHPKIAVSAGVRGLMLLLSPSDLIAVVKGTTADILE"
BASE COUNT          591 a          987 c         1097 g          628 t
ORIGIN      
        1 gccttcgtgg cggttacggt ggccatctcc tttgccaccc tggttgtggg agagttggcg
       61 cccaagaacc tggccataat gcgtgcggag ccaatagcgt tgacactatc cggccccgtg
      121 aatttcctct ccagggtagc agccccgctg gtatgggttc tcacatctac cacggggctg
      181 gtgatgaagc tggtaggcgg accggaccga gtgcggatct ctcccattac ggaggaagag
      241 atcatggcga tggtagcgag tggcgaggag gcgggagtag tcgagcccac cgagcggaag
      301 ttgatcgacg aggtattcga gtttggcgat accatcactg aggaggtgat ggttcccagg
      361 gtagatgttc gcgcgctttc gagggatacg acggtggccg aggcgcgcaa agctatagtg
      421 gaaacgggcc acactcggct gccggtctac gagggcagtc tggataacat catcggggta
      481 atccacgcca aggacgtgct gcaggagttg ccccccgacg cccccgacga acaggtgaac
      541 cgtgcggtca cttcaatcat gcggagggca tatcacgtcc ccggctcgaa gcgggtggcg
      601 gagttgcttc cggagttgca gcggcagcaa ctgcacctcg cggtggtggt ggacgagtat
      661 ggaggcactg cggggatcgt gactctggac aacctgcttg aggagatagt cggcccaatc
      721 agggacgagt acgatgcacg cgaggagccg gatatccaga tgatcggcga ggagcaggcg
      781 gtggtgagcg gcggtgcgga cctgggtgac gtgggcagcg cactggaggt ggatctggag
      841 accgaaggcg tcgacaccat aggcggattg gtgtacgccc ggctggggcg aatccctgtg
      901 gagggggact cggtggaatt gccggatgcc ctggtggagg tgatctccat gcggggccgc
      961 cgcgtgtgga aggccagggt gactcgccga gagctgcccc cagaggagga agaatagccc
     1021 gagccgctta cagtcccaga cccgggtcga tcgatgccag catgcctgtc agcgcgcctg
     1081 tgagcatatc ccagccggag gagtggccga tctccagcaa gccggggagt tcgtccagtg
     1141 ctccaccagg ttcagggtcc ctcagcaccc ggaggacgaa gcgatggagc ggctcggcgg
     1201 catgaccctc cgccatatca ctcatgaggg tgtggctgat ccagttggtc tgagaccgtg
     1261 ccatgtctac cagttccgcg gacggggtca gccgccacct gcacaggccg gggtacgcta
     1321 gatccatctg agagagtgcg aagaggacgg ctcccagata gtcgtcgcct gatggcgtga
     1381 gtccgggccc aaggcctacc agtcccctac ccgcctgaac catggcgccc ccgtcatggt
     1441 gtatgcaggc atccgcgacc gccgacactg cccccagcgc ctttcccggg atgagtggat
     1501 tcgtctccga ggattcccgc ggcaccagtg tggttgagca cgaggcggta tgtccgtttg
     1561 tgccgttgcc ggatgggcgg ctggtgtctg ggagcagtgc gcgcaggaac ggcatcgcct
     1621 gcgccagccc atcgctacag cactgaggga tcaccgactc caggatggat accaggttcg
     1681 ctgccaggac aggaagggga attgcatcgt cggggcggat ccgctctggt cgccacaact
     1741 gggcaccggc ccagtcgata gagaggccct ctccaaggag cagccgccgt ccctggttcc
     1801 tgaacaccgt tccaccggga acctggcgca tccccagagg cacctgaagg catcgacgat
     1861 gtgccacgtg gcctgtcggt cctatccaga gcaactctcc acccataccc tggaggtaga
     1921 cggcgttggt caccaccgcg ttcaccctgc cccagaaggc agggctctcc agcagcgcta
     1981 gagccttctc tccgattaga cttgcagtgg ccgactccct gaagctcatg ggagcgagga
     2041 tagggtggtg acggttgtgg tgtcaaggat gcagcgaatt cgatgggcag gggcgacgca
     2101 agcgtcgccc ctgcccatcg ggatcactct ggtttggata tgtagggtgg caccccctgc
     2161 atatcgaggt tctggtaggg cgacccgccc tactaggtca agggcacgtg ccctgcgatt
     2221 cacgcgaacc atgatgctgc gtaatgggtg cgtgctgctc cacacgctac tgcaaccgtc
     2281 cggctaggct tcctctctgt gaggactatc caaccggaag aagttgctca tgtccccgtt
     2341 gctcttgtgt tcctggaaga ggaagaccag gtcccgctcg tcgaaggtgc ggaaccattc
     2401 ctcccaactg atgggctgga gcccctgtcc cccgtagccc gggaagttga agcgcagcac
     2461 gccgggtctt ccctcatgct cggtgcccgg cacagttgcg ggttgcccac ccctctcctc
     2521 tgcccactgc tggatcacct cgtggctccg agtgcctagc gactctccgg ggcgctcttc
     2581 gtgctcctgt ggcgagcgga tccagatgga gttctgcgtc gtcttggaga gttgcccgcc
     2641 gaacttatgc aggaactgct cgtcactcat gcgagcatgc gtggtggagg gctgcctacc
     2701 cttcccgtgg ggttgtgccc ccgatggatg gtggggagtc tgatggctgc cgccgccacc
     2761 ttgtgatgcc gcggacatgc cctgctgtcc cttgcctctt tgcgtcatcc gttcacctcg
     2821 ctgtcgattg aagtcggagg cgcgggagac gcagaggacg tgccagaggc gcgggagcta
     2881 ctccagaata tccgcggtgg ttccttttac tacggcgatc agatcggagg gagacagcag
     2941 cagcattagt cctcgaaccc cggcgctgac tgctatcttg gggtgtttga gcgcggtctg
     3001 atccatgaat accgggtagg cgcgtctact gccaaggggg gagaccccac ccttcacgaa
     3061 gcccgtgaga cgcgtgatct cggacgggtc caccatctct gccctcttgt cacccatctg
     3121 ctgtgccagc tttcgcagac tcagcgtctg gtcgcccggt accagcgcca ggacaactcc
     3181 atttcgctcg ccccgaacga tcagtgtctt gaacaaggtg tcgggggaca tggtgagccg
     3241 ctcggccgcc tccttggcgg gggtgaagcc gtcgtgctcg aaggatcgga cctcgtacgg
     3301 gac
//
LOCUS       DATALS010000028         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_271718, whole genome shotgun sequence.
ACCESSION   DATALS010000028 DATALS010000000
VERSION     DATALS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271718"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VHS06_00585"
     CDS             <1..190
                     /locus_tag="VHS06_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986662.1"
                     /translation="LPRLSRNLLLEILVRHAQKGDRILVEDGRLVGLLSRSNVIGYLQ
                     VREELGISGRDRGGKRAA"
     gene            215..889
                     /locus_tag="VHS06_00590"
     CDS             215..889
                     /locus_tag="VHS06_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX2986663.1"
                     /translation="MPLFEDRSDAGRRLAAELEEYRGKDALVLALPRGGVEVGYEIAT
                     ALSLPLDVFVAKKLGAPGEPELAIGAVAENGGEWVDKDSVALLGVTDEYLKQEIATQT
                     REMERRVRVYRGGTPLPALAGKTVLLVDDGIATGYTILAALSGLRAANPGNLVAAVPM
                     AAEESLWRVSRESDRVVCLVTPEPFYAVGYHYAEFGQLTDEDVVRYLERARQELSTSR
                     GQTRGS"
     gene            913..1131
                     /locus_tag="VHS06_00595"
     CDS             913..1131
                     /locus_tag="VHS06_00595"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEX2986664.1"
                     /translation="MAEERIDRSDVQPGAIVDYIEGVEYPCSKADIINQAVMRGAPQM
                     VIDALQRLPDEQYYDLAYLMDRFSRVSP"
     gene            complement(1188..2384)
                     /locus_tag="VHS06_00600"
     CDS             complement(1188..2384)
                     /locus_tag="VHS06_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2986665.1"
                     /translation="MPRQWIQTLIGLLVLVPILAARLMRTPAPVVRVENAHGVTYRQL
                     RHLVVGTVALVVGVIILAWVAIRTVEVLTLLAISVVLAVALRPTADRLNGLRIPFTMR
                     RMPRALAISLIYLFLAAVAAGIGFLAIPPLIVETEKLITSFPAYIAQLSDYLEFLRRY
                     PFVPDLGALESQLVNQLVGNLAQAITILLFAVNVLSGMLSIGVVLLLTFFLIYDAELI
                     YEHLISLVPITRRETARHMTSRMGYKIEGWLKGTITLAVVLGVSTGVAMWLLGMPYPI
                     LLGLAAGIFELVPMIGPYLGAAPAVIAALFIGPTWRLIAVILFFIAIQQLENNVLAPT
                     IMGRQVDLPPLLVILALLIGAAIRGIVGALLAVPVMAVLQVLWLDLVVPEIRQRVDRV
                     DQGPAP"
BASE COUNT          542 a          690 c          880 g          474 t
ORIGIN      
        1 tctcccgcgt ctgagtcgca atctcctgct tgagatactc gtccgtcacg cccagaaggg
       61 cgaccgaatc cttgtggaag atggacggct ggttggactg ctcagccgga gcaacgtaat
      121 tggctacctc caggtgaggg aggaactggg catctctgga cgggatcgag gaggcaagag
      181 ggcagcgtga cggggtgcag ggaaggaggg gaacatgcct ctctttgaag accggagcga
      241 tgcgggaaga aggcttgctg ccgaactgga ggagtatcgc ggcaaagatg ctctggtgct
      301 tgcgttgccg cgagggggcg ttgaggtcgg ctacgaaatc gccaccgccc tatcgctgcc
      361 gctggacgta ttcgttgcaa agaaactggg ggctccgggc gagcctgagt tggctatagg
      421 ggcggttgcc gaaaacggcg gagagtgggt cgacaaggac tcggtcgccc ttctgggcgt
      481 gacggacgag tatctcaagc aggagattgc gactcagacg cgggagatgg agcgacgggt
      541 tcgtgtctac cggggaggaa cccctcttcc cgcgttggcc ggcaagaccg tgttgctcgt
      601 cgacgacggg attgccacag gttacacaat actggccgct ctgagtggac tgagggcggc
      661 caatcccggg aatctcgtgg ccgctgtgcc gatggctgca gaggagtccc tctggagggt
      721 gtcccgggag tcagaccgcg tagtgtgcct ggttacgccg gagccgttct acgccgtggg
      781 ctaccattat gcagagttcg gccagctcac agacgaagat gtggttcgct acttggagcg
      841 ggcgcgacag gagctttcga cgagtagagg ccagacacgg ggcagttgag aatagcggag
      901 ggagactgat agatggcgga ggagaggata gacaggagcg acgtccagcc tggggcgatt
      961 gtggactaca tcgagggggt cgagtatccc tgctccaagg cagatatcat caatcaggct
     1021 gtcatgcgcg gagcgccgca gatggtgatc gatgcccttc agcgactgcc ggatgagcag
     1081 tactatgatc tcgcgtactt gatggaccgg ttcagccggg tgagtccgtg acggtagttg
     1141 tacttcgtta ggacgtggaa aatgaggggc cggctaaatc gctcgtgtta gggagccggc
     1201 ccctgatcca ctcggtctac ccgctgcctg atttccggaa ccaccagatc caaccatagt
     1261 acctgcagaa cggccatcac cgggacggcc aacagtgcgc cgacgattcc tctgatagct
     1321 gcgccgatca gcagcgcgag gatgacgaga agcggaggca ggtccacctg ccttcccatg
     1381 atagttggtg ccaggacgtt gttctccaac tgctgaatgg ctatgaagaa gagaatgacg
     1441 gcgatcagtc tccaggtggg cccgatgaac agggcggcga tcactgccgg tgcggcgccc
     1501 aggtatgggc cgatcatcgg gaccagttcg aagattcccg cggccagtcc gagcaggatt
     1561 gggtagggca ttcccaaaag ccacatcgcc actcctgtgg acacacccag caccacggcc
     1621 aatgtgatgg tccccttcag ccatccttcg atcttgtagc ccatcctcga cgtcatgtga
     1681 cgggcggttt cgcgcctcgt gatgggcacc agggagatga ggtgctcgta tatcagttcg
     1741 gcgtcgtaga tcaggaagaa ggtgagcagc aggacgacgc cgattgagag cattccgctc
     1801 agaacgttca cggcaaagag gagtatggtg attgcctggg cgaggttgcc taccagttgg
     1861 ttcaccagtt gcgactccag cgcaccgagg tcgggcacga aggggtagcg ccggagaaat
     1921 tccaggtagt cactgagttg ggcgatgtag gcagggaagc tggtgatcag tttctcggtt
     1981 tccactatga gaggtgggat cgcgagaaac ccaatgccgg cggcgaccgc tgccaggaag
     2041 agatagatga gcgagatcgc cagtgccctt ggcatccttc tcatggtgaa cggtattcgg
     2101 agcccgttca aacggtccgc ggtggggcgt agagcaacgg cgaggaccac ggagattgcc
     2161 agcagggtga ggacctcaac cgtccggatg gccacccagg cgagaatgat taccccgact
     2221 acgagggcaa cggtgccgac gaccaggtgc cttagctgtc gataggtcac gccatgggcg
     2281 ttctcgaccc tgacgacggg ggcaggcgtg cgcatcaatc tggctgccag gatgggcacc
     2341 aataccagga gaccgatcag tgtctgaatc cattgtctag gcatgcctgc agcccaccat
     2401 cctcgtgatg tcgggcacaa tcccgacgtt ctgcggacac cgccggtggc gcggaacgag
     2461 agcaaacgca attgggggac cagccccttc agaggctggg ggatggggag aggcgcagcg
     2521 tgacgatgct gttgctgccc gcatggaact gcaacctgtt cgaatccagc agcggcaact
     2581 cctggc
//
LOCUS       DATALS010000029         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_183086, whole genome shotgun sequence.
ACCESSION   DATALS010000029 DATALS010000000
VERSION     DATALS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183086"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VHS06_00605"
     CDS             <1..272
                     /locus_tag="VHS06_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEX2986666.1"
                     /translation="FNRRDFHLAELGGSDAHHLAQIGSGFTIFKGRTCIDFERCLSHR
                     KTKARFGESGSVTVSEHARQVFKSWVEKPTRGLRASLASYYHPST"
     gene            567..>1940
                     /locus_tag="VHS06_00610"
     CDS             567..>1940
                     /locus_tag="VHS06_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2986667.1"
                     /translation="MSGSASGVRRRPSTGWGWPILRDAPLGVVLRQHQGESMEPSRQS
                     HVRLLGLGTLALSVPLLIVVLTTRGVVKTIRKLTASTRAIAQRKFDEPSTIQGSDEIA
                     ESGRTLDLMRRRLQRSMADIRGCNARLERCVQERTSELERSRAELEQSRDYLQTLIDR
                     LSILNSIGVTVNRSLDLGTTLSGALTDTLKLMGMKAGAVYLVDEQSGELELAAHHGLS
                     LEAARVAANLGLSASLCAAAAQAGEPLVVEDTTRYARGSRSVLCTENMRCMVRVPFKS
                     SSGHLLGTMCLGTSVSRSFSKAEVDLLVSVGNQVAAAVQNARLYEEIRRKGRMRGELL
                     EKVIAAQEAERKRIARELHDQTSQALAALAVAVETAAAQAEAGENVSGSLAKMKLLAV
                     DTLEEVHRLIFDLRPTLLDDLGLISALRWYVETRLGEVGIKVRLEVGGEERRLAPQIE
                     TALYRVVQ"
BASE COUNT          350 a          572 c          658 g          360 t
ORIGIN      
        1 tgttcaaccg ccgcgacttc catctggcgg agttgggcgg cagcgatgct caccaccttg
       61 cacagatcgg ctcgggcttc accatcttca aggggcgcac ctgcattgac ttcgagcgct
      121 gcctgagcca tcgcaagacg aaggcgcgat tcggcgagtc gggctcggtc acggtgagcg
      181 aacacgcccg gcaggtgttc aagagttggg tggagaagcc cacccggggt ctgcgcgcct
      241 ccctggccag ctactaccac ccctcgacgt agagtcgtca ttccgttcaa cgcccaaccg
      301 cagggactac ccgatggttt tgatgggctg gccatcttct agtagggcgg gtcgcccctg
      361 acccgcccgt ctcttgccgt ggcccgggcg ggtcagggac gacccgccct actattcacc
      421 ctacccgcgg tgccccagtc aggggcatga caactcctag aatatgcgta cctaccgcac
      481 gtaagctcca attgaagcaa cagtatagtt gagccatcga agttggtgta gaatcggccc
      541 cgaacccttt tgcggggagc ggtgcgatga gcggctcggc gtcaggcgtg aggcgacgac
      601 cttcgacggg atggggctgg ccgatcctca gggacgcgcc gctgggggtt gttctccggc
      661 agcatcaggg ggagtcgatg gagccttccc ggcagtctca tgtgcggcta ctgggtttgg
      721 ggacgttggc gctctcggtt cctctgttga tagtggtttt gaccacccgt ggtgtggtga
      781 aaacgattcg gaagctcaca gcctccaccc gtgccattgc ccagagaaag ttcgatgagc
      841 cttcgacgat ccaggggagc gatgagatcg ccgagtcagg gcgcacgctc gacctcatgc
      901 gccgcagact gcaacgctcc atggcggata tcaggggctg caacgctcgg ctggagcgct
      961 gcgtccagga acgcacctcc gagttggagc gctccagggc cgagctggaa cagtccaggg
     1021 attacctgca gacgttgatc gaccgtctct cgatactcaa ctccatcggc gttactgtga
     1081 accggtcact ggatctcggc acgaccctca gcggtgccct gaccgatacc ttgaagctta
     1141 tggggatgaa ggccggggcc gtctacctcg tggacgagca gtcgggcgaa ctggagcttg
     1201 cagctcatca tgggctttcg ctggaggcgg ctcgcgtggc agccaatctc ggcttgagcg
     1261 caagcttgtg cgccgcggcc gcgcaagccg gcgagccgct ggtggtggag gataccactc
     1321 gatacgctcg aggctcgcgc tcggtgctgt gcaccgagaa catgcgatgc atggtgcggg
     1381 tcccattcaa gtcctcctcc ggccacctgc tgggcaccat gtgccttggt accagcgtgt
     1441 ctcgaagctt ctctaaagcc gaggtagatc tgctggtctc ggtcggcaac caggtcgccg
     1501 cggcggtgca gaacgcccga ctgtacgagg agatccggcg gaagggacgg atgaggggcg
     1561 agttgctgga gaaggtgatc gcggcccagg aagctgagcg caagcgcata gcccgggagt
     1621 tgcacgacca gaccagccag gcgctggcgg ccctggctgt ggcggtcgag acggcggctg
     1681 cacaggcgga ggccggagag aacgtgagcg gcagcctggc gaagatgaag ttgttggcgg
     1741 tggataccct cgaagaagta caccggctga tcttcgacct tcggccaacg ctgctggatg
     1801 atcttggact gatttccgcg cttcgatggt acgtggagac ccgactggga gaagtcggca
     1861 tcaaggtgcg tttggaggtg ggaggcgagg agcggaggct ggctcctcag atcgagacgg
     1921 cgctgtaccg cgtcgtgcag
//
LOCUS       DATALS010000030         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_2954,
            whole genome shotgun sequence.
ACCESSION   DATALS010000030 DATALS010000000
VERSION     DATALS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2954"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="VHS06_00615"
     CDS             <1..1498
                     /locus_tag="VHS06_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2986668.1"
                     /translation="WTTNDRERVLELTAQRGLDEADVARIRRVGYDSSLLAGRVVRER
                     RIQLIPRLDNVPPEDAVLRFWKPSGYQCLVAVPLNARGHLVGVMTYLARPPCRFREDG
                     LAAVQTIADIMGSGLENARLHTELEREIAERRLVEASSRESDELFRNFFEEAPLGTGI
                     IATDLHFVRANPALCRMLDYAESELVGRSFLDLTHPEDAGRDRELSARLFRGEIPSYG
                     VEKRLLKKSGEPLWVSVTAAAIHDSHGKPLYGLGMVEDISDRKAVEREREELLASREA
                     YVHTISHDLRSPLTVIQGQAQMLRRLLDMDDQEAASRRSVEAIFTGARRMNTMIQDLV
                     DSARMESGQLRLSREALDLEAFVCELLERFGEVMECSRVRVKAPERLPRVAADPDRLE
                     RIVVNLLSNALKYSPQGSSVTVQIRQRGGEVVISVIDYGRGIPPEEIASLFQRFRRVG
                     GERKEGLGLGLYITKGLVEAHGGRIWVQSEVGKGSTFSFTLPVVTDGR"
     gene            complement(1541..2344)
                     /locus_tag="VHS06_00620"
     CDS             complement(1541..2344)
                     /locus_tag="VHS06_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX2986669.1"
                     /translation="MGKDGVVLGLVGSPNRDGRTNRMVRAALEGAAAAGARTELVQMA
                     DHRVEACKDCQPWVCNVNLKCSYEDPAFEFLSEKIRSCDALVLGTPIYWWDTSGLVSH
                     LVLKMFRCFARNGPMNGLPALGIGVAGGTGNGLVTGLRPIYHFFQTLQMRAIEPLPVT
                     RFNIDAAEQHAREQGALLAKRAREPQPFEGVEERLLWYDALPYLGLSRMGERRFLAAL
                     AVAAAGDHARPEVARGLATADALAASGNTLGAMQEITRVYNAAVKAFES"
     gene            complement(2573..>3042)
                     /locus_tag="VHS06_00625"
     CDS             complement(2573..>3042)
                     /locus_tag="VHS06_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HEX2986670.1"
                     /translation="NYEQQPDVVETLKQMTAGRGPDACIDAVGMEADSPGPDNLFDRA
                     QQAMRTQMERPHVVREAAMACRKGGVLSIMGVYMGFADKIPLGAMMNKGLTIRTAQQH
                     GHRYLGRMLEHVQRGEVDPARVVTHRLPLEEAERGYRLFKNKEEGCIRVILQP"
BASE COUNT          493 a          951 c         1042 g          556 t
ORIGIN      
        1 ctggaccacg aatgaccgag agcgagtgtt ggagttgacg gcccagcgag gactggacga
       61 ggccgacgtt gcccgcattc gtcgtgtggg ctacgactcc tcccttctgg ccggaagagt
      121 ggtgcgcgag cggcggatac agctgatccc ccggctcgac aatgtccccc cggaggatgc
      181 ggtcctccgt ttctggaagc cttcggggta ccagtgcctc gtagcagtcc ccctcaatgc
      241 ccgcggacac ctggtgggcg tgatgaccta tcttgcccgg ccgccatgcc gcttccggga
      301 ggatgggctg gcggccgttc agaccatcgc agacatcatg ggctccggat tggagaacgc
      361 ccgactgcat accgagttgg agcgggagat cgcggagcgg cggctggtgg aggcgtcgtc
      421 gcgggagagc gatgagctct tccgcaactt cttcgaggag gcgccgctcg gcacggggat
      481 cattgcgacc gacctccact tcgtcagggc caatccggcg ctgtgccgga tgctggacta
      541 cgccgagtcc gagcttgtgg ggcgtagctt cctggacctg acccaccccg aggatgccgg
      601 cagggaccgt gagttgtctg cgcgcctgtt ccgcggtgag atcccgagct atggggtaga
      661 gaagcgcctc ctcaagaaga gcggcgagcc gctgtgggtc agcgtgacgg ccgcggccat
      721 ccacgatagc cacggcaagc ctctctatgg cctgggaatg gtggaggata tcagcgatcg
      781 caaggcggtg gagcgggaga gggaggagtt gctggcgagc cgggaggcat acgtccacac
      841 catctcgcac gacctgcgtt cgcctttgac tgtcatccag gggcaggcgc agatgctccg
      901 gaggctgctg gatatggacg accaggaggc cgccagccgg cgaagcgtgg aggccatctt
      961 tacgggtgcg cggcggatga acacgatgat ccaggacctg gtggactccg cgcgcatgga
     1021 gtcgggccag ctgaggctca gtcgggaggc cctcgatctc gaggctttcg tgtgcgagtt
     1081 gctggagcgg ttcggcgagg tcatggagtg ctcgcgggtg cgtgtgaagg cgccggagcg
     1141 acttccccgt gtggccgccg accccgaccg gctggagagg atcgtggtga acctgttgag
     1201 caacgcgttg aagtactctc cacagggatc ttcggtcacc gttcagataa ggcaacgagg
     1261 gggcgaggtg gtcatctccg tgatcgatta tggcagaggg atcccccccg aggagatcgc
     1321 ctcgctcttc cagcgcttcc gaagggtagg tggcgagcgc aaggagggac tggggctggg
     1381 actgtacatc acgaaggggt tggtggaggc acacggggga cggatctggg tgcagagcga
     1441 ggtcgggaag ggcagcacgt tcagtttcac gttgcccgta gtcacagacg ggcgttgagt
     1501 gctgaacgtt gggtgctgag tgctgccctc acccctggcg ctagctctcg aacgccttca
     1561 ctgccgcatt gtagacgcgg gtgatctcct gcattgctcc caaagtgttg ccgctggctg
     1621 ccagagcgtc ggcggttgcc aggccccgag ctacttccgg gcgggcgtgg tcgccggccg
     1681 ccgccacggc gagggctgcc aggaatcggc gctcccccat gcgactcagc ccgaggtagg
     1741 gcagcgcgtc gtaccagagc agccgctcct ccacgccctc gaagggctgg ggctcccgcg
     1801 cgcgctttgc cagcagggca ccttgctccc ttgcgtgctg ctctgcggcg tcgatgttga
     1861 atcgggtcac cgggagcggt tcgatggcgc gcatctgcag tgtctggaag aagtggtaga
     1921 tggggcgaag accggtcacc agcccgtttc ccgtgccccc cgcgacgccg atgccgagcg
     1981 caggcagacc gttcataggg ccgttcctgg cgaagcagcg gaacatcttc aacaccaggt
     2041 ggctgaccag cccgctggtg tcccaccagt agatgggggt gcccagcacc agggcgtcgc
     2101 aagagcggat cttctcgcta aggaattcga aggcgggatc ctcgtagctg cacttgagat
     2161 tgacgttgca cacccagggc tggcagtcct tgcatgcctc caccctgtgg tcggccattt
     2221 ggaccagctc ggtcctcgct cctgccgccg ccgccccctc cagagccgcc cttaccatgc
     2281 ggtttgtcct gccatcgcgg ttggggcttc ccaccagacc caagacgacg ccgtctttac
     2341 ccatttcctc tcacctccga gccgcttctc caacgattgt cgcaagtgca gccggcgagg
     2401 cccatccatc gccggcaagc cgcctcaggc atcataggaa gaccggtgcg gggagtcaac
     2461 gagcgacgat ctcgaccggt agggcagttc gcgggtgccc gtggccgcga ccaggtccgg
     2521 gatgtgggca gagggacctc caccgcgttg atatgaggtt acggctaaag ggttacggtt
     2581 gaaggatcac ccggatgcag ccctcctcct tgttcttgaa cagcctgtat ccacgctccg
     2641 cctcctcgag cggcaggcga tgagtcacca ccctggccgg atcgacttcg cctcgctgga
     2701 cgtgctccag catgcggccg aggtagcggt ggccgtgctg ctgggccgtg cggatggtga
     2761 gccccttgtt catcatagcg cccagcggga tcttgtctgc gaaccccatg tacaccccca
     2821 tgatggagag cacgccccct ttgcggcagg ccatggcggc ctcccggacg acgtgggggc
     2881 gttccatctg ggtgcgcatc gcctgctgcg ccctatcgaa gaggttgtcg ggccccgggc
     2941 tgtctgcctc catccccacc gcgtcgatgc aggcgtccgg acctcggccc gcggtcatct
     3001 gtttcagcgt ctccacgacg tcgggttgct gctcgtagtt ga
//
LOCUS       DATALS010000031         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_363154, whole genome shotgun sequence.
ACCESSION   DATALS010000031 DATALS010000000
VERSION     DATALS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363154"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="VHS06_00630"
     CDS             <1..1879
                     /locus_tag="VHS06_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX2986671.1"
                     /translation="AYGYTGKILRLNLTTGTISVDEHDDLWYRRYLGGAGIAAYYLLN
                     ETKPGIDPLGPENKLIFAIGPLTGLALSGASRICVGAKSPMTGGIGKSEAGGFFPAEL
                     KHAGYDAIVVEGKSEKPVYLWIKDGEVEIRDAGHLWGRGVMETEDAIKEETGEKQARV
                     MSIGPGGEKQVRYACIIADLKDAAGRGGMGAVMGSKNLKAIAVRGHKMPETKDPETIK
                     ELAKMMAKDAPVKAKGFHDWGTGPGMPGWNLVGNLCVRNFQDAYMENLEPITAQAIKS
                     TIRIGMEACYACAVRCKKVVKVDEPWVADPRYGGPEYETIGAFGTSTGVDNVKAIARA
                     HHLCQHYTIDTISTGVTIAWAMECYERGIFTKADTDGLDLHFGNAEAMLKLIEMIGER
                     RGFGNLLAEGTRVASRKVGKGSEELAMNVKGVEIAMHDPRYKQGLSIGYSVANIGGDH
                     CGGLHDSNYEVDGPALVNDGHPLGFYEPMPVNELSTRKAFTFFQRHRWTVQQDSAVVC
                     IFVPYTYEEHVKLINAATGWNASLEELLLTGQRAITMGRIYNLREGLTAEDDRLPKRF
                     FSPPLKGELAKNGIAVDPEKMAECISKYYYFMGWDPKTGVPTQVTLEKLGLDWAANQL
                     A"
     gene            2069..>2697
                     /locus_tag="VHS06_00635"
     CDS             2069..>2697
                     /locus_tag="VHS06_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2986672.1"
                     /translation="MQSPLLSTKLYVPPAQPEVVLRPRLIEQLKEGLARRLTLISAPA
                     GFGKTTLLSQWLAQCGQAIAYVALDEGDNDLARFLSYVIVALGKADPSFGAAAKSALP
                     ILSHQPVEIVLTALINDAAEAGRDSVLVLDDYHAVESPAVHKAVAFLLDHLPRRMHLV
                     IASRADPPLPLARLRAQGQLAELRAADLGFTRDETAEFLRRMPDLEVSA"
BASE COUNT          541 a          893 c          830 g          433 t
ORIGIN      
        1 ggcgtacggt tacaccggca agatactccg tctgaacctc accaccggta cgatctcggt
       61 ggacgaacac gacgatctct ggtaccggcg ttacctcggc ggtgccggca tcgccgccta
      121 ctatctgctg aacgagacca agccggggat tgatcccctg gggccagaga acaagctgat
      181 cttcgccatc ggtcccctca ccggcctcgc cctctccggc gcgtcccgca tctgcgtcgg
      241 ggcaaagagc ccgatgaccg gcggcatcgg caagagcgag gcgggaggct tcttcccagc
      301 tgagttgaag cacgccggct acgacgccat cgtcgtcgag ggcaagtccg agaagccggt
      361 ctacctttgg atcaaggacg gcgaggtcga gatccgagat gccggccacc tctggggccg
      421 gggcgtcatg gagaccgaag acgccatcaa agaagagacc ggagagaagc aggcccgcgt
      481 catgtccatc gggccgggcg gcgagaaaca ggtccgctac gcctgcatca tcgccgacct
      541 gaaggacgcg gccggccgag gcggcatggg cgccgtgatg ggctcgaaga atctgaaggc
      601 catcgccgtc aggggccata agatgcccga gacgaaggac ccggaaacca tcaaggaact
      661 ggccaagatg atggccaagg atgcccccgt caaggcgaag ggcttccacg attggggcac
      721 cggccctggc atgcccggct ggaacctcgt tggcaacctc tgcgtccgca acttccagga
      781 cgcctacatg gagaacctgg agccgatcac agcccaggcc atcaagtcca cgattcgcat
      841 cggcatggag gcatgctacg cctgcgccgt ccgctgcaag aaggtggtga aggtcgacga
      901 gccgtgggtc gccgatccga ggtacggcgg gccggagtat gaaaccatcg gggccttcgg
      961 caccagcacc ggcgtcgaca acgtgaaggc gatcgccagg gctcaccatt tgtgccagca
     1021 ctacaccatc gacaccatct ccaccggcgt gaccatcgcc tgggcaatgg agtgctacga
     1081 gcgggggatc ttcaccaagg ccgacaccga tgggctcgac ctgcacttcg gcaacgccga
     1141 ggcgatgtta aagctgatcg agatgatcgg cgagcggaga ggcttcggga acctgctggc
     1201 cgagggaacc agggtggcgt ctcggaaggt cggtaagggc tccgaggaac tggcgatgaa
     1261 cgtcaagggc gtcgaaatcg ccatgcacga tccgcgctac aagcagggcc tctccatcgg
     1321 ctactccgtg gcgaacatcg gcggtgatca ctgcggtggc ctccacgaca gcaactacga
     1381 ggtcgacggc ccggcgctgg tgaacgacgg ccaccccctc ggcttctacg agccgatgcc
     1441 ggtgaacgag ctctccaccc gaaaggcatt caccttcttc cagcgccacc gatggacggt
     1501 ccagcaagac agcgctgtag tgtgcatctt cgtcccctac acctacgagg agcacgtcaa
     1561 gctgatcaac gccgccaccg gctggaacgc cagcctggag gagttgttgc tgacaggcca
     1621 gcgggcgatc accatgggcc gaatctacaa cttgcgcgag gggctcaccg ccgaggacga
     1681 ccggctgccc aagcgcttct tctcgccgcc cttgaagggc gagctggcca agaacgggat
     1741 cgccgtcgac ccggagaaga tggccgagtg cataagcaag tactactact tcatgggctg
     1801 ggacccgaag accggcgtcc caacacaggt cacgctggag aagctgggcc tggattgggc
     1861 ggccaaccaa ttggcgtagc cataacaatg tgattggggg cccgcatcga tcgatgcggg
     1921 ccctttccca tgtccatatc ctgtctatcc gtctcgtcct gtgcatcctg cccgaatcgg
     1981 tcccctcacg tagccgcgca ccaccaaaag gccggactca agaagaacgg gtggtataat
     2041 ctgcccggag gtagattaga gcaccaggat gcagagccca ctcctctcga ccaagctgta
     2101 cgtgcccccg gcacagcccg aggttgtgct gcgccctcgt ctgatcgagc aactgaagga
     2161 agggcttgcc cgaagactca cactcatctc cgccccagcc ggcttcggca agaccaccct
     2221 gctgagtcag tggcttgccc agtgtgggca ggcaatcgcc tacgtggcgc tcgacgaggg
     2281 ggacaacgac ctcgcccgct tcctttccta cgtcatcgtc gccctcggca aagccgaccc
     2341 tagcttcggt gcggctgcga agagcgcgct cccaatcctc tcgcaccagc cggtcgagat
     2401 cgtcctcacc gcccttatca acgacgcggc tgaagcaggt cgggactccg tcctcgttct
     2461 cgacgactat cacgccgtcg aatccccggc ggttcacaag gcagtcgcct tcctgttgga
     2521 tcaccttccc cggcggatgc acctggtgat cgccagccgt gccgatcctc ctctgcccct
     2581 cgcccgcctg cgcgcccagg ggcaactcgc cgagttgcgc gccgccgacc ttggattcac
     2641 ccgcgatgag accgccgagt tcctgagacg gatgccggat ctcgaggtat cggccga
//
LOCUS       DATALS010000032         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_46582,
            whole genome shotgun sequence.
ACCESSION   DATALS010000032 DATALS010000000
VERSION     DATALS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46582"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VHS06_00640"
     CDS             <1..262
                     /locus_tag="VHS06_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986673.1"
                     /translation="FTAPPMLNAFRNHQPYVYWWVSEGVAKSPMPAWHTRMSETERWQ
                     VITYAWSLGEQGTAGASSPGGRGASGPDGHPGTTRVRDDTGF"
     gene            311..694
                     /locus_tag="VHS06_00645"
     CDS             311..694
                     /locus_tag="VHS06_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986674.1"
                     /translation="MSPEQRKTLLDDRENYPMRVRFLGKVGIMTQEELAFLRENEIDF
                     DVDDRGAAAVLGPDAVRAAQPQAARVAREGTTRGAGVVHGAPPGQQESLVRREARWIG
                     SGCLLLFLRVAVSIASAILRRLTPR"
     gene            1238..1960
                     /locus_tag="VHS06_00650"
     CDS             1238..1960
                     /locus_tag="VHS06_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2986675.1"
                     /translation="MHTPVATIMVADHDAQDRCRLRGCLERADYRVRDVSDGGSLLLA
                     LEREMPHLVVLDLQLPSVGGMELCRRIKRHGDVPLVIVTALEDEEVKLRTLDLYAEDY
                     IQKPARDAEVVARVRRVLQRTLLSCLPPSSTVYVDDSLSLDFFRREARTPGGVSRLTP
                     LESRLLQLLVRNSGQLLPNQLILERLWGDQNSCAGSLWEYVRRVRHKIGDDANRPRYL
                     LNEPGLGYRFSPVTRQAMGGTR"
     gene            1960..>2241
                     /locus_tag="VHS06_00655"
     CDS             1960..>2241
                     /locus_tag="VHS06_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2986676.1"
                     /translation="MGPRDGTERGPLYHNIMVAVDNSPCSGFAVDRAVELAAGTKARL
                     TGYHVYAANLHDTRFRQLEPGLPESYRPEAMLNRLRSIHNDLIGHGLGVI"
BASE COUNT          409 a          667 c          728 g          437 t
ORIGIN      
        1 tttcaccgcc cctcccatgt tgaacgcgtt ccgcaatcac cagccatacg tctactggtg
       61 ggtaagcgag ggggtggcca agtcgcccat gcctgcgtgg cataccagaa tgagcgagac
      121 cgaacgctgg caggtgatca cgtatgcctg gtcgctgggg gagcagggca cggccggtgc
      181 ctcgtctcca ggaggccggg gcgcctctgg ccccgacggc catccaggca ccactcgggt
      241 cagggacgac acgggtttct aagagacggg accaccgtga cggtcgatag ttgccatgtt
      301 gtactcgtgc atgagcccgg aacaacggaa gacgcttctg gatgatcgcg aaaactaccc
      361 catgcgggtg cgctttctgg ggaaggttgg gatcatgacc caggaggagt tggctttcct
      421 ccgggagaac gagatcgact tcgacgtcga cgaccgtggc gcggccgctg tgctggggcc
      481 cgacgcggtc agggcggccc aaccgcaggc cgccagggta gctcgtgagg gaacgactcg
      541 gggagccggc gttgttcatg gtgcgccgcc tgggcagcag gagtcgctgg tccggcgtga
      601 ggcgcgttgg attggctcag ggtgtctact tctctttcta agagttgccg tttcgatcgc
      661 ctctgccatt ctgaggcgtc ttacccccag gtgaagtgcg cttgtatgct tctgacatag
      721 tactcctgat cctagaggtc agatccatcg tgccagagaa gtagtcctcc gccacgctgg
      781 atctgacctc caggagtcgg gggcgtccct gaccccgacg gtcccctaga ccaccgcccg
      841 ggtcagggac gacccgggct cctagaaatc gggagtcccc tagaccaccg cgtgggtcag
      901 ggacgcggcg tcgcggtcac gccgggttcc tggtgtcggg atcgtcgtgg tcgaacggta
      961 atgctctgcc acagtagaag tgacacgtac tgtcatgctg agcgaagcga agcatctcct
     1021 cgtgaacgga gagatccttc gctttcgctc aggatgacat ccgtcacgat catagcggtc
     1081 gagtagcagt ggcacctcta cctccgtccc tcgttgccgg cacccatctc ccatgcccgc
     1141 tggcacagga attgccgctt cattgaagca gaagctggct aactcgggtc gtacggaaga
     1201 tacaatcctc agtccttgtt atccctggag accattgatg catacaccgg tagccaccat
     1261 catggtggcg gaccatgacg cacaggatcg ctgccggttg agaggctgcc tggagcgcgc
     1321 ggactaccgg gtgagggatg tatccgacgg cggaagcctt ctcctggcgc tagagaggga
     1381 gatgccccac ctggtggtgt tggatctaca gttgccgtca gtcggtggca tggaactgtg
     1441 ccggcgtatc aagcggcatg gcgatgtgcc gctggtgata gtcacggcgt tggaggatga
     1501 agaggtcaag ctgcggacgc tagatctcta tgccgaggac tatatccaga agcctgcacg
     1561 ggatgccgag gtcgtggcga gggtccgccg cgtattgcag aggaccctgt tgtcgtgcct
     1621 gccacccagt tccaccgtct atgtcgacga ttcgctcagc ctggacttct tccgcaggga
     1681 ggctcgcaca cccggtgggg tgtccagatt gaccccgctg gagtctcggt tgctgcagct
     1741 gttggtgcgc aactcggggc agttgctccc gaaccagttg atactggagc gactctgggg
     1801 cgaccagaat tcctgcgcgg ggagcctctg ggagtatgtc cggcgagtcc gccacaagat
     1861 aggcgacgat gccaacaggc ctcgatacct cctcaatgag ccggggcttg gctatcgctt
     1921 ctctccggta acacgccagg ccatgggggg caccaggtga tggggccccg tgatggcaca
     1981 gaacgcggcc ctctctatca caacataatg gtggcggtgg acaattcacc ctgctcggga
     2041 ttcgccgtag acagggcggt tgagttggcg gccggcacga aggctcggct caccggctac
     2101 cacgtctacg cggccaacct tcacgacact cgcttcagac agcttgagcc gggactgccc
     2161 gagtcctacc ggccggaggc gatgctgaac aggcttcgct ctattcacaa cgacctgatc
     2221 gggcacgggc tcggcgtgat c
//
LOCUS       DATALS010000033         5128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_138006, whole genome shotgun sequence.
ACCESSION   DATALS010000033 DATALS010000000
VERSION     DATALS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138006"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VHS06_00660"
     CDS             complement(<1..247)
                     /locus_tag="VHS06_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986677.1"
                     /translation="MLRPAILFLDTNILLDCPRLEDYRAAGRALTLVVIPEVMRELHG
                     LSRAPERGRTSSALQAFSMLANSALQVDVEGCVMLGDP"
     gene            complement(344..502)
                     /locus_tag="VHS06_00665"
     CDS             complement(344..502)
                     /locus_tag="VHS06_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986678.1"
                     /translation="MRRRSLTFLGDALGWEPLWSYLLALSLLLLPIEVGLRRIRTLPF
                     TRHPNDDS"
     gene            762..1157
                     /locus_tag="VHS06_00670"
     CDS             762..1157
                     /locus_tag="VHS06_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2986679.1"
                     /translation="MVQRPVLIASAARDPFVSELVKILQSEGWEVLALHTVGAVQKAV
                     SCRQPALVVLDTMLPGGDALSLCRQWKSDPATAEIPVFFFSVLMARDRCVEAGADGFM
                     LKPTEQEQLLEQLRQLLNSRVTKLHRRAK"
     gene            1231..2709
                     /locus_tag="VHS06_00675"
     CDS             1231..2709
                     /locus_tag="VHS06_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2986680.1"
                     /translation="MPRNRRTIEKPSAMAGTDGSNSHWIEDSLSHVRELSNHVAELST
                     LMGISTLLSADAPLLQGLEAVCRLSAEICQAQVCFIHLADGEDDLVCVARHTPTDAHE
                     YTWEGIARIYGRKATQKGEMVSCANLLLRQEGQGDTPHMGGICAVPLKGKSRPVGAIG
                     VGFTGTHRFSAREKDILNAVAAQVAVAVERSWLFDQLQEQLARARSLRQVATQIASNL
                     DLDLVLDAIVSHASRLLAAEYSAIFLNDHAEKATDSEGLGGEREQMHPSSVHLEDSPL
                     GRAVRQARETAQPAIVQSLEPYPSHKPPTEMKPNEYRVVLAVPLLSHDEVLGALALCY
                     LERRRFDSSDIALVEDFAGQAALAIRNARLYQEAMEDRLSLESAIDQINTHGISLMDD
                     NLNLKFANPAAFWLLGVNPRRSEMSLDQWKALCKKGLAEEADLDQVVEQIRANPEQTV
                     TAKLSVRGSSDTPKTLQLLSLPFRQADGTIRGRVNLIESTEC"
     gene            complement(2758..3453)
                     /locus_tag="VHS06_00680"
     CDS             complement(2758..3453)
                     /locus_tag="VHS06_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2986681.1"
                     /translation="MDVIWGGLTQAFWLLVSGDPEVVGVTLLSLQVSGTATLLSLLVG
                     IPLGTALALNQFPGRRLVVSLVNTGMGVPPVVVGLVVTIMLWRNGPFGEFRLLYTPAA
                     MVIAQTVIAAPMVTGITVAAMGSLNPKLRLQILALGATRWQMLWLLMREARLPLLAAV
                     MAGFGAVISEVGASMMVGGNVLGQTRVLTTATVMESSKGNFDVAIALSVILMLMVYGV
                     NVLLTYAQQRRPA"
     gene            3651..4997
                     /gene="folP"
                     /locus_tag="VHS06_00685"
     CDS             3651..4997
                     /gene="folP"
                     /locus_tag="VHS06_00685"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX2986682.1"
                     /translation="MITARVLSLPTESDVREEASLLGVPFASLEPLVGTLGLQAVVLQ
                     GIPTQTAAFLKAMVPSHLGLIVASQAGNPHPSPLPEGERVELSHLPVGEGSGARSILP
                     LSTQHSALSTSTLLIAAPPPLLRDMASRLEAAGDDTERAAAAALRTALDRYLGRQLGT
                     TLCRNLTLEWGSRTYVMGIINVTQDSFSGDGLGPNVEAAVEQGRRFVEEGADILDVGG
                     ESTRPGFQQVPVEQEIARVVPVVRRLAQEFAIPISVDSYKLPVMQAALDAGANMVNDI
                     WGLRTEGLADLTAAYGVPIVLMHNRRGEITTSLIGGHFRGVQYHDLMGEIVQGLQGSI
                     DLALQASIKWENIIVDPGLGFGKTPEQNLVVMRRLRELCSLGRPILMGTSRKSVIGKV
                     LGLPPEERVEGTAATVAVSIANGADIVRVHDVKAMSRVARMTDAITRVPSTTHELP"
BASE COUNT         1010 a         1792 c         1492 g          834 t
ORIGIN      
        1 agggatcgcc caacatgaca catccctcga catcgacctg tagtgcactg ttcgccagca
       61 tggagaatgc ctggagggcg gagctggtcc gcccgcgctc gggtgcccgc gagaggccgt
      121 gcagctcgcg cataacctcg gggatcacca ccagggtgag tgcgcggccc gccgcccggt
      181 agtcctcaag ccgagggcaa tccagcagga tgttggtgtc caggaagagg attgccggcc
      241 gcagcatatg acttctccgt gcagagcggg tgttacgatt gcatcaggag tatccactat
      301 tatggtgtgg ccgtccagag gggagcggga tgggtggggt gggttaggag tcgtcgttgg
      361 ggtggcgggt gaagggtagg gtgcggatgc ggcggagccc gacctcgatc ggcagcagca
      421 gcagggatag ggcgagcagg taggaccaga gcggctccca acccaatgca tcgccaagga
      481 acgttaaaga tcttcttcgc accttagcat cctgacggca ataccaccta gaagcttcta
      541 ctactagcta ttcatgctag ctgctcatct aacatccctg gttattgatt taacccattg
      601 ccatatccga ctctgtattc cctttgccaa aggattgctg tatactctcg cgttgtccgg
      661 aacctgacta ctcgtagcgg cccattggag agccatcggt ctgcggctgc cacgaggccc
      721 ttcagatccg gagacagcgt gcagttgata gaggagagcg catggtccag cgcccagtgc
      781 tcatcgcatc cgcggcaagg gacccttttg tatcggaact ggtgaagatc ctccagtccg
      841 aagggtggga ggtactggca ctccacaccg ttggtgcggt ccagaaggcg gtaagttgca
      901 ggcaaccggc cctggtagtc ctggacacca tgctccctgg cggcgatgcc ctctccctct
      961 gccgacaatg gaagagcgac cccgcgaccg ccgagattcc ggtcttcttc ttcagtgtgc
     1021 tgatggctcg cgatcgatgc gtggaggccg gagccgacgg ctttatgctg aaacccaccg
     1081 agcaagagca actcctggag cagcttcgac agctgctcaa cagccgagtg acgaagctcc
     1141 atcgccgcgc aaagtagcgt ggccggatcc ccaaagccct gcacacggcc gcagagccac
     1201 cacaacacta caccgttcat ggaggccccg ttgcccagaa accgccgcac tatcgagaag
     1261 ccctcggcga tggccggcac ggatggttcc aacagccact ggatcgagga tagcctcagc
     1321 catgtaagag aactctcgaa ccacgttgcc gaactctcca ccctgatggg gatatccacc
     1381 ctcctctccg ccgacgcgcc cctgctccag ggcttggagg ctgtttgccg tctcagcgcc
     1441 gagatctgcc aggcccaggt ttgcttcatt cacctggccg acggcgaaga cgatctggtg
     1501 tgcgtggccc gccacacccc gaccgacgcc cacgaataca cttgggaagg gatagccagg
     1561 atctacggcc gcaaggcaac ccagaaggga gagatggtct cgtgcgccaa cctgctgctt
     1621 cgacaggagg ggcaggggga taccccgcat atgggcggca tctgtgcggt gccgctaaag
     1681 gggaaatcgc gtccggtagg tgcgattggc gtgggcttca cgggcacaca ccgcttctct
     1741 gctcgggaga aggatatcct caacgccgtg gcagcccagg tggcggtggc ggtggagcga
     1801 agctggctct tcgaccagct ccaggagcaa ctggcgcggg cgcgcagcct tcggcaggtc
     1861 gccacccaaa tcgcctccaa cctggatctc gacctcgtgc tggacgccat agtcagccat
     1921 gcatcccgac tgctggcggc cgagtacagc gccatcttcc tgaacgacca tgctgagaag
     1981 gccaccgatt ccgagggact tggcggtgag agggagcaga tgcatcccag cagcgtccac
     2041 ctcgaggaca gccccctcgg cagagcagtc aggcaggcaa gggaaaccgc ccaacccgcc
     2101 atcgtgcaga gcctggagcc ctacccatcc cacaagcccc ccacggagat gaaacccaac
     2161 gagtaccgag tggtgctggc ggtccccctg ctctctcatg atgaagtgct aggggccttg
     2221 gccctctgtt acctggagag acgccgcttc gacagctcag acatcgccct ggtcgaggat
     2281 ttcgcgggac aggcagcact ggccatccgg aacgcccgac tgtaccagga ggcgatggag
     2341 gacagactat ccctggagtc ggccatcgac cagatcaaca cccatggcat ctccttgatg
     2401 gacgacaatc tgaacctcaa gttcgccaac ccagcagcct tctggcttct gggagtcaac
     2461 cctcggagat cggagatgtc actggatcag tggaaagccc tctgcaagaa gggtctcgcg
     2521 gaggaggccg atctggacca ggttgtcgag cagatccggg ccaaccccga gcagacggta
     2581 accgcgaagc tctccgtccg gggctcatcc gacactccca aaaccctgca gctgctcagc
     2641 cttcccttcc gccaggccga cggcaccatc cgaggccgcg tcaacctcat cgaaagcact
     2701 gagtgctgag tcggctccct ctccctctgg gagagggtcg gggtgagggc cgtcccccta
     2761 cgccggcctg cgctgctgcg cataagtaag cagcacattg acaccgtaaa ccatcagcat
     2821 caatatgacg ctcagcgcga tcgccacgtc gaagttgccc ttggacgact ccatcaccgt
     2881 cgccgtggtc agcaccctcg tctgccccag cacgttacca cccaccatca tggacgcgcc
     2941 aacctcggag atcaccgccc cgaaccccgc catgaccgcg gccagcagcg gcaacctcgc
     3001 ctcccgcatc agcagccaca gcatctgcca ccgcgtggcc cccagcgcca ggatctgcag
     3061 ccgcagcttc gggttgaggg aacccatcgc cgccacggtg ataccggtga ccatcggggc
     3121 ggcaatcacc gtctgagcga tcaccatggc agcgggcgtg tacagcaggc ggaactcccc
     3181 aaaagggccg ttccgccaaa gcatgatcgt caccaccaga cccaccacca ccggcggcac
     3241 ccccatgccg gtgttgacca ggctgaccac caaccgccta cccggaaact ggttgagggc
     3301 gagggccgtg ccgaggggaa tacccacgag caggctgagc agcgtcgccg tgcccgagac
     3361 ctgcagcgac agcaacgtca cccccaccac ctcgggatcc cccgaaacca gcaaccagaa
     3421 cgcctgagtc aaccctcccc agatcacgtc catccaaaag actccatcat cggattgccg
     3481 cgcacttcct ccaacagccc gcgtcacgcc cagatcatac catccccagc agcagccctg
     3541 cccccaccca atgaggatcg actcttcatg tcaccacaat cggctctaac gcccgttcct
     3601 gtatactgtc tatagaacca tccgaatcac ctttggggag acccaccttc atgatcaccg
     3661 cacgcgtcct atcgctgccc accgagtccg acgttcgcga ggaggccagc ctgctcggcg
     3721 tgcccttcgc cagcctcgaa ccactcgttg gcaccctggg gctccaggca gtcgtgctgc
     3781 aggggatccc cacgcagacc gccgcattcc tgaaggcgat ggtaccctca cacctgggtc
     3841 tgatcgttgc ttcccaggca ggaaaccctc accccagccc tctccctgag ggagagaggg
     3901 tagagctctc ccatctccct gtgggggagg ggtcgggggc gcgttcaatc ctcccactca
     3961 gcactcagca ctcagcactc agcacttcca ctctcctcat cgccgcccct ccgcccctgc
     4021 tgcgcgacat ggcctcccgc ctggaagccg ccggcgacga cacggagcgc gccgccgccg
     4081 cggcgctccg taccgccctg gaccgctacc tcggccgcca actcggcacc accctatgcc
     4141 gcaacctcac cctggagtgg ggcagccgca cctacgtcat ggggatcatc aacgtcaccc
     4201 aggactcctt ctccggcgac ggcctcggcc ccaacgtcga ggctgcggtg gagcagggcc
     4261 ggcgcttcgt tgaggagggc gcggacatcc tcgacgtagg gggcgagtcc acccgccccg
     4321 gcttccagca ggtaccggtc gagcaggaga tcgctcgggt cgtccccgtc gtccgccgcc
     4381 ttgcgcagga gttcgccatc ccgatctccg tcgactccta caagctgccg gtgatgcagg
     4441 cggccctgga cgcgggcgcc aacatggtca acgacatctg gggcctgcgc accgagggac
     4501 tcgccgacct caccgccgcc tacggcgtgc cgatcgtcct gatgcacaac cggcgcggcg
     4561 agatcaccac aagcctcatc ggcggccact ttcgaggggt gcagtaccac gacctcatgg
     4621 gtgagatcgt ccaggggctg caagggagca tcgacctggc gctccaggcc agcatcaagt
     4681 gggagaacat catcgtcgac cccggcctcg gcttcggcaa gacccccgag cagaacctgg
     4741 tcgtgatgcg gcggctgcgc gagctttgct ccctcggccg gccgatcctg atgggcacct
     4801 cccgcaagtc ggtcataggc aaggtgctgg gcctccctcc cgaagaacgg gtggagggca
     4861 cggccgccac cgtcgccgtc agcatcgcca acggtgccga catcgtccgc gtccacgacg
     4921 tcaaggccat gtctcgggtc gcccgcatga ccgacgccat cacccgagta ccaagcacaa
     4981 cccatgaact accctgagtc actcgcctac atcgacagcc tgaccgacta cgagaagagg
     5041 accggctacg cctactctgc cgcgaccttc gacctgaggc gcccgcgcgc actgctcgcc
     5101 ctcctgggcg acccgcacct ccgcttcc
//
LOCUS       DATALS010000034         3832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_46677,
            whole genome shotgun sequence.
ACCESSION   DATALS010000034 DATALS010000000
VERSION     DATALS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46677"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VHS06_00690"
     CDS             complement(<1..229)
                     /locus_tag="VHS06_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986683.1"
                     /translation="MPRRRPRGGKRRQGIRGQGTGDRRSSIRRPSIPGPHSPTPIPNP
                     SAPTSHTELPPHAFVNASEEEFAHILDFYRVK"
     gene            complement(272..1477)
                     /locus_tag="VHS06_00695"
     CDS             complement(272..1477)
                     /locus_tag="VHS06_00695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2986684.1"
                     /translation="MAGTSQVVLSPSRGRSWFRRDVAVSLLLGVMIAQAVLLGVNMAD
                     QFSHPFGPVDWWTYFSSVSMLQAGHGQSLYDVPSLSAFQHSLGRETAVVYPYHPTFLL
                     IAYPFVLLGPSLSYSGWVLFNLALMAGCLRVLAGALVPRADWRLFFLLVAASLPVYIA
                     LSTGQSSFVVCLGLTLLTIGMLRDSNLAIASGLVILLIKPQYIPVWLILILWRRKFRA
                     IAWAATGGLVVLAASYVMVGARGLADYVRLLVSGPLSGAYSLSHTLNGLAHDVAPAGV
                     AIYIYLLLSAVALGVYILLLKRAGHDLEPRITLSFTILVSMLVANYLVIYDLTIWAIP
                     LALLWPAAASTRGKVVLSLAMIAPWVTQMAAQYGVGFVWPTVGLEILVLVLLYRRHRR
                     DGPVYALVS"
     gene            complement(1544..2722)
                     /locus_tag="VHS06_00700"
     CDS             complement(1544..2722)
                     /locus_tag="VHS06_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX2986685.1"
                     /translation="MSSADLKDLASREIDQLAQDLRSISLEIHANPELGYEERLASSL
                     LSNRLEEAGFAVERGIAGMDTAFTATVQGRGSGPKVAIMAEYDALPGLGHACGHNIIG
                     TSAVGAVLGLRAVMDSIDGTVVVMGTPAEEMIGGKIRMVDAGLFRDIDAAIMFHPRNA
                     TQLGRLFTATTNVEMIFHGKSTHAAASPDQGINALDACIATFNGTNALKKHLADGVRL
                     HGVIFEGGTAPNIVPDRARAVFSVRARTRVYLDKVLEKVRACAEAGAMMAGATVEVNV
                     KTICPEVNTNRALAEAFGRNAIALGHDPLPIDMNSGGGSTDMGAVSQVVPAIHPHFKV
                     TSANAELHSKEFQVAAGSEEAQDAMIQGAKVLAMTAIDIFTDSDLLRRAKEEFAQSVR
                     "
     gene            2901..3074
                     /locus_tag="VHS06_00705"
     CDS             2901..3074
                     /locus_tag="VHS06_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986686.1"
                     /translation="MTAQIKCARCKEPIQGEPVVKAGKIYCSEACAFEAIKRPRPSTC
                     GRDIPMVSDQPKV"
     gene            3272..>3832
                     /locus_tag="VHS06_00710"
     CDS             3272..>3832
                     /locus_tag="VHS06_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX2986687.1"
                     /translation="MPWSLSRPSRTPPARQEESDEELEERVRRGLFFSRLEDLLAWGR
                     KNSIWPFNFGLSCCYVEAATSLTPKWDLARFGAEVLRSSPREADLMIVAGTVFIKMAP
                     VVQRLYEQMMEPRWVISMGSCANSGGMFDVYSVVQGVDKFLPVDVYVQGCPPRPDTLI
                     EGIMLLRDQVGKEKRPLSWTIGPQGVI"
BASE COUNT          743 a         1191 c         1202 g          696 t
ORIGIN      
        1 acttcacgcg gtagaagtcc aggatgtggg cgaactcctc ctcgctcgcg ttgacgaagg
       61 cgtgaggggg aagctcagta tgggatgtcg gggccgaggg attggggatg ggtgtcggac
      121 tgtgaggccc gggtatggag gggcgccgaa tcgaggacct cctgtcccct gttccctgcc
      181 cccttattcc ctggcggcgt ttgccaccgc gtggcctgcg cctcggcatt acctcgcgac
      241 cccatctccc atcacagcat ctgttcttcc ctcatgatac cagtgcatac accggtccgt
      301 ctctccgatg ccgtctatac agcagaacga gcacgagtat ctcgaggccg actgtcggcc
      361 agacgaagcc gactccatac tgggcagcca tctgcgtgac ccagggggca atcatggcga
      421 gcgagaggac gactttgccc ctggtgcttg cggctgctgg ccacagcagc gcgagcggta
      481 tggcccagat ggtcaggtcg tagatcacca ggtagttcgc gaccagcatg gagaccagaa
      541 tcgtgaacga aagcgttatc cgaggctcga ggtcgtgacc cgcccgcttg agcagaagga
      601 tgtagacccc gagggcgact gcactcagca gaaggtagat gtaaatcgcg acgcccgcgg
      661 gcgccacgtc gtgggcaagc ccgttcaagg tgtggctcag tgagtaggcg cccgagagag
      721 gccccgagac cagcagtctt acgtagtcgg ccagacccct cgcacccacc atgacataag
      781 atgcagccag caccacgagc ccaccggtgg cggcccaggc gattgccctg aacttccggc
      841 gccagaggat caggatcaac cagaccggga tgtactgggg cttgatgagc aagatcacga
      901 gacccgaagc aatggcaaga ttgctatctc gcagcatccc gatcgtgagc agggtgagtc
      961 caagacagac cacgaagctc gattgcccgg tcgagagagc gatatacacc ggcagcgaag
     1021 cggccacaag aaggaagaac agcctccagt ccgccctggg caccagtgcg cctgccagca
     1081 ctcgaaggca accggccatc aatgccaggt tgaagagtac ccagcccgag taagacagac
     1141 tgggcccgag cagcacgaag gggtaggcga taagcaggaa ggtggggtgg tatgggtata
     1201 ccacagccgt ctccctgccg agggagtgct ggaaggcgct gaggctcggg acgtcgtaca
     1261 gtgattggcc gtgcccggcc tggagcatcg acaccgagga gaagtaggtc caccagtcga
     1321 cggggccgaa aggatggctg aactggtcgg ccatatttac cccgagcaag acggcctgag
     1381 cgatcatcac cccgagcagc agggacaccg cgacgtcgcg gcgaaaccac gagcggccgc
     1441 ggctggggga aaggacgacc tgcgaggtcc cggccaaagc gcatacctca ggtcaatctg
     1501 ttctggctgc cggattgtcc cacccccccg gccactcatg caactaccgc accgattgag
     1561 cgaactcctc ctttgctcgc cggaggaggt cggagtcggt gaagatatcg atggccgtca
     1621 tcgccaggac cttcgcgccc tggatcatcg cgtcctgggc ctcctcggat cccgccgcaa
     1681 cctggaactc cttgctgtgc agttccgcgt tcgcgctcgt gaccttgaag tgcgggtgaa
     1741 tcgccggcac cacctggctc accgctccca tgtccgtgga acctccgccg ctgttcatgt
     1801 cgattggcag agggtcgtgg ccgagcgcga tcgcgttccg gccgaaggcc tcggcgagcg
     1861 cacggttggt gttcacctcc gggcagatcg tcttcacgtt cacctcgacc gtagccccag
     1921 ccatcatcgc tcccgcctcg gcgcaggcgc gcaccttctc cagcaccttg tccaggtaga
     1981 cgcgagtccg tgccctgacg ctgaagactg ccctcgccct atcggggacg atgttcggcg
     2041 ctgtgccgcc ctcgaagatc acgccgtgca ggcgcacccc gtcggccagg tgcttcttca
     2101 gagcgttggt gccgttgaag gtggcgatgc aggcgtcgag cgcgttgatc ccctggtccg
     2161 gcgaagcggc cgcgtgggtg gacttgccgt ggaagatcat ctcgacgttg gtcgtcgcgg
     2221 tgaacagcct tcccagttgg gtggcgttac ggggatggaa catgatcgcg gcgtcgatgt
     2281 cccggaacag cccggcatcg accatcctga tcttcccgcc gatcatctcc tctgccggag
     2341 tccccatcac cacgaccgtg ccgtcgatgg agtccatcac cgcccgcagt cccagcacgg
     2401 ctccgacggc ggaggtgcct atgatgttgt ggccgcaggc gtggccgagg cctggaaggg
     2461 catcatactc cgccatgatc gccactttcg gtccgctgcc ccttccttgc acggtggcag
     2521 tgaaggccgt gtccatgccc gctattcccc gttccacggc aaacccggct tcctccaggc
     2581 gattggacag caaagaggag gcgaggcgct cctcgtaccc gagctcgggg ttggcgtgaa
     2641 tctcgaggct tatggagcgc aaatcctgcg ctagctggtc aatctctcgc gatgccaggt
     2701 ccttcagatc cgctgaactc atctcgccac ctccctgttg ttggcctggc gggacttctc
     2761 tcagcacttc cgtcccgcgg atggggaacc ttaacctcat tactagatac gagcaagctc
     2821 gataagtaga atagcctagc agtagaggct ttgaccaggc taacgtaccc tgcggcataa
     2881 agttctcgtg gaggtgagac gtgactgctc agatcaagtg cgcccgctgc aaagagccga
     2941 tacagggaga gccggtagtg aaggctggga agatttactg ctcagaagca tgcgccttcg
     3001 aggccatcaa gcggccgcgc ccatccacct gcgggcgcga tatccctatg gtgagcgacc
     3061 agcccaaggt ctagggaatt caatacctcc gttacaagaa accgtggcct gtaaagtcag
     3121 cgcctccccc tgccctcctc gcggcatcca tccgtacacc tgtagctcca tatccgtgcg
     3181 ataaggcacg tatcttgcgc gcccagaacc tgctccagaa cacacaccgg cgagacatcg
     3241 tcgagagcca ctgactggaa aggatcacct tatgccctgg tctctcagta gacccagcag
     3301 gactcctccc gcgaggcagg aggaaagtga cgaggaactg gaagagcggg ttcgccgagg
     3361 tctcttcttc tcccggttgg aggatcttct tgcgtggggc aggaagaact cgatctggcc
     3421 gttcaacttt gggctgtcct gctgctacgt ggaggctgcg acgagcctga cccccaagtg
     3481 ggaccttgcg aggtttgggg ccgaggtctt gaggtcctcg cccagggagg ccgatctcat
     3541 gatcgtggcg gggacggtgt tcatcaagat ggcccccgta gtgcagaggc tctacgagca
     3601 gatgatggag ccgcgctggg tgatcagcat ggggtcctgc gccaactctg gggggatgtt
     3661 cgacgtctac agcgtggtgc agggggtgga caagttcctt cccgtggacg tctacgtcca
     3721 gggctgcccc ccgaggccgg ataccttgat cgaggggatc atgctcttac gggaccaggt
     3781 cggcaaagag aagcgccctt tgagttggac gatcggccct cagggcgtga tc
//
LOCUS       DATALS010000035         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_407153, whole genome shotgun sequence.
ACCESSION   DATALS010000035 DATALS010000000
VERSION     DATALS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407153"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1471)
                     /locus_tag="VHS06_00715"
     CDS             complement(20..1471)
                     /locus_tag="VHS06_00715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2986688.1"
                     /translation="MAFTPNLDAAFVTYQKDRISHWDGVARQLDSWSGWGGEYHRRLT
                     EIYRFLVAPGQKVLEIGCARGDLLAALKPSVGVGIDFSREMIERAAKRHPQLRFIQAD
                     AHDLTCLDESFDVIILSDLVNDLWDVQTVLEQVSRLATPQTRVILNSYSRLWELPLAM
                     AEKLGLGRPTLYQNWLTVEDMANLLHLADLEVIQDRAEIMWPLPLPILGPLANRYLVR
                     LWPFKILGLTNFIVARPIAKPLSEGEDPTVSVVVPARNEAGNIAQIIARTPQMGRSTE
                     LIFVEGHSTDNTYDAIEQELAKHPELDSRLMRQRGKGKGDAVRLGYAEAKGDILMILD
                     ADMTVPPEDLPRFYEVLRSGKGEFANGVRLVYPMEKQAMRFANLLGNKFFSLAFSWLL
                     GQSVKDTLCGTKVLWKRDYERIAANRSYFGDFDPFGDFDLLFGAAKLNLKIVDLPIRY
                     RERKYGTTNIQRWKHGLLLLRMVLFAARRIKFV"
     gene            complement(1471..2481)
                     /locus_tag="VHS06_00720"
     CDS             complement(1471..2481)
                     /locus_tag="VHS06_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX2986689.1"
                     /translation="MGTSWKDSRAFWKDKRAIVTGGGGFLGSFVTSKLTERGTAQVIV
                     PRSREYDLRDLDAIRRLLADTSIPGESDSGRQPVDVIIHLAAVVGGIGANRERPAEFF
                     YDNLMMGAQLLHEAYRAGVKKFVAVGTVCAYPKFTPVPFREEDLWNGYPEETNAPYGL
                     AKKMLLVQSEAYRQQYGYNSIFLMPVNLYGPGDNSDPRSSHVIPALIKKCVDAVESSA
                     SEIVVWGDGSPTREFLYVEDAAEGIILAAERYDRSDPVNLGSAFEISIRDLAQIIAEE
                     TGFTGKIVWDTSKPNGQPRRKLDTSRAEEGFGFRARIDFRTGLRQTIAWYQGQRRENR
                     VP"
BASE COUNT          524 a          815 c          811 g          617 t
ORIGIN      
        1 gtggggagcc cgggcggtgc tacacgaact tgatgcgcct tgccgcaaag agcaccatcc
       61 tcagcagcag aagaccgtgc ttccatcgct ggatgtttgt cgttccgtac tttcgctctc
      121 tgtagcgtat gggcaggtcg acgatcttga ggttcagctt ggccgcgccg aacagtagat
      181 cgaagtcgcc gaaggggtcg aagtccccga agtatgagcg gttcgccgcg atcctctcgt
      241 aatctctttt ccacagcacc ttggtgccgc acagggtatc cttgacggat tgtcccagca
      301 accaggagaa ggcgagactg aagaacttgt tgcccagaag gttggcaaag cgcatggcct
      361 gcttctccat cggatagacc agcctcacac cgttggcgaa ctcacccttg cctgaccgta
      421 gaacctcgta gaagcgaggc aggtcctccg gcggaacggt catgtcggca tccaggatca
      481 tcagtatgtc gccctttgct tcggcgtatc ccagccgtac agcgtctcct ttccccttgc
      541 ctctctgccg catgagccgg ctatccaatt cagggtgctt ggccagctcc tgctcgatcg
      601 cgtcataagt gttgtccgtg gagtgcccct ccacgaagat cagttcagtg cttcttccca
      661 tctgcggtgt gcgggcgatg atctgcgcta tgttgccggc ttcattgcgt gcagggacga
      721 ccaccgagac ggtggggtcc tctccttccg acaacggttt ggcgatgggg cgtgccacga
      781 tgaagttggt gagcccaagg atcttgaatg gccagagtcg caccaggtat cggttggcga
      841 ggggacccag gatcggcagc ggcagcggcc acatgatctc cgccctgtcc tgaatcactt
      901 cgaggtcggc cagatgcagc agattggcca tgtcttccac tgttagccag ttctggtaga
      961 gcgtcgggcg gcccagcccc agtttctcag ccattgcaag gggaagttcc cacagccggc
     1021 tgtaggagtt gaggatgacc ctggtctggg gtgtggccag cctggacacc tgttccagaa
     1081 ccgtctggac atcccacaga tcgttgacca gatcggagag gattatgacg tcgaagctct
     1141 cgtccaagca ggtgaggtcg tgggcatcgg cctggatgaa gcgcagttgg ggatgccgtt
     1201 tggccgcacg ctcgatcatt tcgcgagaga agtcgatgcc gactcccacg gagggcttca
     1261 gtgctgccag caggtcacct ctggcacagc ctatctcaag caccttctgc cccggtgcta
     1321 cgaggaagcg atagatctcg gtgagccttc tatggtattc gccaccccac ccactccaac
     1381 tatccagttg ccgggcaacc ccatcccagt ggctgatccg atccttctga taggtgacga
     1441 aagcggcgtc gagattgggg gtgaaagcca tcaaggcacc ctgttctcgc ggcgttgccc
     1501 ttggtaccag gcgatggtct gccggagccc tgttcggaag tctatccgag ctcggaagcc
     1561 gaaaccctct tcagccctgc tggtgtccag tttgcgcctg ggctgtccgt tgggctttga
     1621 tgtgtcccac acaatcttac cggtgaaacc ggtctcttcc gcgattatct gtgccagatc
     1681 gcgaatggat atctcgaagg cggagcccag gtttacggga tcgcttcgat cgtacctctc
     1741 tgcggccagg atgatccctt cggcagcatc ttcgacgtag agaaactccc gcgttgggct
     1801 tccgtcaccc cagacgacga tctcagaagc gctggattcc accgcatcga cgcacttctt
     1861 gataagcgcg ggtatcacgt gggaggagcg tggatcggag ttgtcgcccg gaccgtacag
     1921 attgacgggc atcaggaaaa tggagttgta gccgtactgt tggcgatagg cctcagattg
     1981 caccagaagc atcttcttgg ccaggccata gggagcattg gtttcttcgg ggtacccgtt
     2041 ccagagatct tcctccctga atggaaccgg agtgaacttg ggataggcac acaccgtccc
     2101 taccgccacg aatttcttca cgccggcacg atacgcctcg tggagcagtt gcgcccccat
     2161 catcaggttg tcatagaaga actcggccgg gcgttctcgg tttgccccga tgccaccgac
     2221 caccgcggcc aggtgaatga tcacatccac cggctggcgt ccactgtccg actcccccgg
     2281 aatcgacgta tcggcaagga gacggcggat ggcgtcgagg tccctgaggt cgtactcacg
     2341 gctccgcggt acgatcacct gggccgtgcc tcgctctgtc agtttcgaag tgacgaagga
     2401 gccgaggaaa cctccgccac ctgttacgat cgcccttttg tctttccaga atgctctgct
     2461 atctttccag gaagtgccca tagaaatccg ctcctggtat ctatatcact ttgagtttag
     2521 gcgcgtttcg cagcggctct gctcgcgtat cggttgcgag tcagagccgg gatgcaggtc
     2581 gtgtctgttt acaatttcgt gagaacgtga ggtgttttca caagtagact gtcgacggtg
     2641 gcggaggttt cctcggctcc ttcgtcactt cgaaactgac agagcgaggc tcggcccagg
     2701 tgatcgtacc gcggagcagg gagtacgatc taagggacat tgacgccatc cgccgtctcc
     2761 ttgccta
//
LOCUS       DATALS010000036         4319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_407209, whole genome shotgun sequence.
ACCESSION   DATALS010000036 DATALS010000000
VERSION     DATALS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407209"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VHS06_00725"
     CDS             <1..976
                     /locus_tag="VHS06_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2986690.1"
                     /translation="SRDPDRPLEELRGSIPRSLDAIIEALSTRSFEAVDRYLADISQL
                     RWQLGFDIGEVIEGLLLLREAVLPHLRTGLHPGSDEAYQTVAAFDECLRYTLGRFGHL
                     YAQAIRKHLEEQQRHTAIMEERQRLARALHDSVTQSLYSMTLYTEAAASMLAAGNHGK
                     VAEHLQTLQETAQDTLSEMRLLIFELHPPILEKEGLVAALQARLDGVEARGGVQTGLL
                     VEGTGVLSITLEEELYRIAQEALNNVLNHARARRVTVRLRLSEEETLLEITDDGIGFD
                     QATVQDRRGFGLSSMDERVRMLGGALQIESRLGEGTRVTARIPGLRRG"
     gene            994..1662
                     /locus_tag="VHS06_00730"
     CDS             994..1662
                     /locus_tag="VHS06_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2986691.1"
                     /translation="MSSHPIRVLVVDDHDIVRKGIQALLEAKEGIEVVAEAANGLEAI
                     AQTEIVRPDVVLMDLVMPKMDGIEATRRIAALHPDAHILVLTSFSTDDKVFPAIKAGA
                     QGYLLKDSGPDDLVQAIRQVYRGEPSLHPTIARKMIHELAHPLDRPPTPDPLTAREVE
                     VLRLVGQGLLNHEIADRLVITEATVRSHVSTILSKLQLASRTQAALYALREGLATLDE
                     GLPD"
     gene            1855..2160
                     /locus_tag="VHS06_00735"
     CDS             1855..2160
                     /locus_tag="VHS06_00735"
                     /inference="COORDINATES: protein motif:HMM:NF025807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986692.1"
                     /translation="MTVDVLLSGRLKIDGYGEGHPTNPDGSFRLSMEEGCTVQKVVHD
                     MGVPPERVAMTMVNGRRTGNGRNLMSGDRVILIPADVACLWRALGRQNMGMESVFDF"
     gene            complement(2157..2399)
                     /locus_tag="VHS06_00740"
     CDS             complement(2157..2399)
                     /locus_tag="VHS06_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX2986693.1"
                     /translation="MIDVRVRVYATLRQYLPTLSVGQSAELSLPEGATVADTLDHLGI
                     PRVEVRNSFVNGLQRELDWPLHDRDELALFPPIGGG"
     gene            complement(2537..>4319)
                     /locus_tag="VHS06_00745"
     CDS             complement(2537..>4319)
                     /locus_tag="VHS06_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2986694.1"
                     /translation="IEMRKSTEGKVEMAKILRVDMTAGTATYEEVPERYQAAGGRWLT
                     SSMVLEEVDPTCHPLGPNNKLVFAPGILAGTSAATSSRLSVGGKSPLTGGIKESNAGT
                     KFAPMQAKLGVKAIVVEGQPKDGKWYSLLVNRDGASLVPADDLVGKGAYETTDTLIAR
                     YGKVGVCCIGPAREMRMANAGICFNDMKDRASRYSGRGGLGAVMGSKGLKAIVVDDAG
                     APGVPIANRELFEQGRKKMVDALMAHDITKPKGGLNSYGTAVLVNIMNEAGGLPTRNF
                     SNGRFDGAARVAGEAIFEGNKQRLGKELYNHACSPGCIIQCSNTWHAADGSEIVSCIE
                     YETDWAFGPNCGIDDLDVIGKLTWQCNDIGLDTIEAGGTVAVAMEAGVLPFGDGQGAI
                     KLLEEVRQATPLGRVIGNGAVATGKMFGVTRVPAVKGQNMPAYEPRAVKGIGVTYATS
                     TMGADHTAGYTIAPEILGVSGKVDPFGVDKGELSRNMQSSTAFIDSSGHCLFIAFAIL
                     DIPSGFEGMVEECAGVVGADWTGADILAIGEDVLKKERAFNEAAGITSAHDRLPEFMR
                     YEPLPPHNVVWDVSDEELDSVHGKLKG"
BASE COUNT          816 a         1340 c         1339 g          824 t
ORIGIN      
        1 ctcacgcgat ccagatcgtc ccctggagga gttgagaggc tcgataccgc ggagcctaga
       61 cgccatcatc gaggccctct ccaccagatc cttcgaggcc gtggaccgct accttgccga
      121 catcagccag cttagatggc agcttggctt cgacataggc gaagtgatcg agggcttgct
      181 gctgcttaga gaggcggtcc tgccccattt gcggactggc ctccaccctg gctcggatga
      241 agcctaccag acagttgctg ccttcgacga gtgtcttcgc tacacattag gccgtttcgg
      301 ccacctctat gcgcaggcaa tccggaagca tctcgaggag caacagagac acacagccat
      361 catggaggag cgtcagaggc tggcgcgggc actccatgac tcagtaacac aatccctcta
      421 cagtatgacc ctctacaccg aggccgcggc gagcatgctc gctgccggga accatgggaa
      481 ggtggccgag cacctgcaaa cgctccagga gacggctcag gataccctgt ccgaaatgcg
      541 gctactcatc tttgagctgc accccccgat tctggagaag gaggggctgg tggcagcact
      601 gcaggctcga ctggatggtg tggaggcgcg aggaggggtg cagaccgggc tactggtcga
      661 agggacaggg gtgctgtcga taactctgga ggaggagctg tatcgcattg cccaggaggc
      721 cctgaacaac gtcctgaacc atgccagggc tcgaagggtc acggtgcggt tgcggctgtc
      781 cgaggaggag acgctgctgg agataacgga cgacggaata gggttcgacc aagcaacggt
      841 gcaagataga aggggattcg ggctatcgag catggatgag cgggtgagaa tgcttggggg
      901 agccctccaa atagagagtc ggttgggcga ggggacgcgg gtgaccgcca ggataccagg
      961 tttgcgccga ggttgatggg acgagaggtg ggaatgagtt cgcatcccat acgggtcctc
     1021 gtagtcgacg accacgatat cgtccgcaaa gggattcagg cccttctgga ggcaaaggag
     1081 ggcatagagg tggtggcaga ggcagcgaac gggctggagg cgattgccca gacggagatc
     1141 gtgcgccctg atgtggtgct gatggacctg gtgatgccga agatggatgg gatcgaggcg
     1201 accaggcgaa tagcagcact ccatcccgat gcccacatcc tggtgttgac cagcttctcc
     1261 accgacgaca aggtctttcc agccatcaag gctggggcgc agggctatct gctgaaggac
     1321 tcgggtccgg acgatctcgt gcaggcgatt cggcaggtct accgagggga gccgtcgctt
     1381 cacccaacca tagcccgcaa gatgattcac gagcttgccc accctctcga tcgccccccc
     1441 acacccgacc ccctcacggc acgggaggtg gaggtgctga ggttggtggg gcaggggctg
     1501 ctgaaccatg agatcgctga tcggctggtc atcaccgagg ccaccgtccg cagccatgtc
     1561 agcaccatcc tcagcaagct gcaacttgcc agccgaacgc aggctgccct ctacgcgctg
     1621 cgtgagggtt tggctaccct cgacgagggc cttcccgact agcgcattgc ctagtcggct
     1681 ccggttccca catgacatct tattgtggat tcccattcca ctcccagcca atggctccac
     1741 acgccccacg tgatacggtg catccggtga aggctaccta gcagatcgaa actcggatca
     1801 gaacaacttt cgctctcgct ctagacatgg agttgggagg aggtagcaac cgtgatgacc
     1861 gtagacgtcc tcctgtctgg gaggctgaag atcgatgggt acggcgaagg acatcccacc
     1921 aaccccgacg gctccttccg gctaagcatg gaagagggct gcacggttca gaaggtggtc
     1981 cacgacatgg gggtaccgcc tgaacgggtt gcgatgacca tggtcaacgg ccgcaggact
     2041 gggaatggaa gaaacctgat gagtggggac cgggtgattc tcatccccgc ggatgtggcc
     2101 tgcttgtggc gggctttggg caggcagaac atgggcatgg aaagcgtctt cgacttctag
     2161 ccaccgccga tggggggaaa gagggcaagc tcgtccctgt cgtggagagg ccaatcgagc
     2221 tcgcgctgca ggccattgac gaagctgttc ctgacctcaa cccgaggtat gccgagatgg
     2281 tccagggtat cggcaaccgt ggccccctcc ggcagagaga gttcggccga ttgcccaaca
     2341 gagagggtgg gcaggtactg gcgcagggtt gcgtagaccc gcaccctaac atcgatcata
     2401 tcgggtcacc atatcgctcg cattcctgct cccaccccgc cattcccgcg cagacgagaa
     2461 gccagggtat tccccagccc cgcgatcctg gattcccgct tgcggcggga atgacggttt
     2521 agtggtgatt ccagcgctac cctttcagct tgccgtggac cgaatccaac tcctcgtcgc
     2581 tcacatccca gacgacgttg tgcgggggca gcggctcgta cctcatgaac tcgggtagcc
     2641 gatcatgggc actggttatg cccgcagcct cgttgaatgc ccgctccttc ttgaggacat
     2701 cctcgccgat ggccaggata tcggcaccgg tccagtctgc gcccaccacg cccgcgcact
     2761 cctctaccat cccctcgaaa ccgctgggga tatccagaat ggcgaaggcg atgaacaggc
     2821 agtggccgct gctgtcgatg aaggcggtgg atgactgcat gttgcgcgag agctcccctt
     2881 tgtccacacc gaatgggtcc accttcccac tgactcccag gatctcaggg gcgatggtgt
     2941 atcccgcggt atggtccgcc cccatggtac ttgtcgcata ggtgacgccg atgcccttga
     3001 cggcccttgg ctcgtaggcc ggcatgttct ggcccttcac ggccggaacc ctggtgaccc
     3061 caaacatctt gccggttgcg actgcgccgt tcccgatgac gcggccaagg ggagtagctt
     3121 gccgcacctc ctccagcagc ttgatcgccc cctgtccatc tccaaatggg agtacgcctg
     3181 cctccatggc cacggccacc gtgcctccgg cctcgatggt atcgaggcca atatcgttgc
     3241 actgccaggt cagcttgccg atgacgtcca ggtcgtcgat tccgcagttg gggccgaagg
     3301 cccagtcggt ctcgtactcg atgcaggaga cgatctcgct gccatcagcg gcgtgccacg
     3361 tgttggagca ctggatgata cagccggggc tgcaggcatg gttgtacagc tctttgccca
     3421 gccgctgctt gttgccctcg aagatcgcct cgcccgccac cctggccgcc ccatcgaagc
     3481 ggccgttgga gaagttgcgg gtgggaaggc ctccggcctc gttcatgatg ttgaccaaga
     3541 cggctgtgcc gtagctgttc agaccaccct tcggcttcgt gatgtcgtgg gccatgaggg
     3601 cgtcgaccat cttcttccgc ccctgctcga acagctccct gttggcgatg ggcacgcccg
     3661 gcgccccggc gtcgtccacg actatcgcct tcagcccttt ggagcccatc acggcgccca
     3721 gccccccgcg gccggagtag cgactggcgc ggtccttcat gtcgttgaag caaatgccgg
     3781 cgttggccat cctcatctca cgggcggggc cgatgcagca cacacccacc ttgccgtacc
     3841 tggcaatcag ggtgtcggtg gtctcatagg cacccttgcc gaccaggtcg tcggccggta
     3901 ccagactggc accatccctg ttcaccagca gggagtacca cttgccatcc ttgggctggc
     3961 cctccaccac gatggccttc actcccaact ttgcctgcat gggtgcgaac ttggtaccgg
     4021 cgttggactc cttgatgccg cccgtcaggg gagattttcc gcccacggag aggcgagagc
     4081 tggtggcagc gctcgtccca gccaggatgc cgggtgcaaa aaccaacttg ttgttgggac
     4141 ccaggggatg gcaggtaggg tccacctcct ccagaaccat ggatgaggtg agccagcggc
     4201 ctcccgctgc ctggtacctc tcgggcacct cctcgtatgt ggctgtgccc gcggtcatgt
     4261 ctacacgaag gatcttggcc atctctactt tcccctcagt cgactttctc atctcgatc
//
LOCUS       DATALS010000037         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_183715, whole genome shotgun sequence.
ACCESSION   DATALS010000037 DATALS010000000
VERSION     DATALS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183715"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VHS06_00750"
     CDS             complement(<1..1260)
                     /locus_tag="VHS06_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2986695.1"
                     /translation="MAETKRLKYFANGEWKESRSGKYMPVTDSSTGSLIAETPACTAG
                     EVSEAVEAAAAAFPAWADKPIAVRTQLLFHFKSLIEQHIEELTFITAQELGKNLDESR
                     GEYVKIIEACETAVAAPMLMQGRSLMNVSTGHDTVMYREPLGVFAGIAPFNFPAMIPF
                     GWFIPLAITTGNTYVLKAASMVPLSSIRLVELLQDAGLPKGVVNMVTCSRNEADILLT
                     HPAVKGVTFVGSTSVGKQVYSKAAAHGKRVQAQTEAKNHGIVLRDASLERAARGIINS
                     TFGCAGMRCMALPVICVENAVADQFIDYLVKFARERVVGCAYDPKTELGPVVSAEHKK
                     FVTDWIAKGVQEGAELLLDGRNVVVPGFEGGFFIGPTIFDRVTEEMSCGREEVFGPVT
                     FIKRIDDFEEGLAIANGNPFANGSCIFT"
     gene            complement(1489..>1723)
                     /locus_tag="VHS06_00755"
     CDS             complement(1489..>1723)
                     /locus_tag="VHS06_00755"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX2986696.1"
                     /translation="GVDTFGMTTMEAARASVTAVRELSHDIGIPETFKDYGVDYSHLD
                     EMVEDTFKSGNIPLNPVKPTRDAIVKIYRQAIG"
BASE COUNT          294 a          529 c          550 g          350 t
ORIGIN      
        1 cgtgaagatg caggagccgt tggcgaacgg attgccgttg gcgatggcca gtccctcctc
       61 gaagtcgtcg atccgcttga tgaaggtgac cggcccgaag acttcctcgc gtccgcaact
      121 catctcctca gtcaccctgt cgaatatggt cggcccgata aagaagcctc cctcgaagcc
      181 cggcacgacg acgttgcggc cgtctagcag cagctcggcc ccctcctgga cgcccttggc
      241 gatccagtcg gtgacgaact tcttgtgctc ggcggagacc accgggccca gttcggtctt
      301 tggatcgtag gcacagccga cgacccgctc cctggcgaac ttgacgaggt agtcgatgaa
      361 ctggtcggcg acggcgttct ccacgcagat cacgggcagg gccatgcagc gcatgcctgc
      421 gcagccgaag gtggagttga tgatccctct cgcggccctc tcgagcgacg cgtccctcag
      481 gacgatgccg tggttcttcg cttcggtctg cgcctggacc cgcttgccgt gggctgcagc
      541 cttcgagtag acctgtttgc cgacggaggt ggagccgacg aaggtgactc ccttcaccgc
      601 cgggtgggtc aggagtatgt cggcctcgtt gcgggagcag gtgaccatgt tgactacgcc
      661 cttgggcagc ccggcatcct ggagaagctc tacgagccgg atgctgctca gggggaccat
      721 cgaagcggcc ttcagcacgt aagtgttgcc ggtagtgatg gccaggggaa tgaaccagcc
      781 gaaggggatc atggctggga agttgaaggg ggcgatccca gcgaagacgc ccagcggctc
      841 gcggtacatg acggtgtcgt ggcccgtgga gacgttcatc aaggatcggc cttgcatcag
      901 catcggggcc gcgacggccg tctcgcacgc ctcgatgatc ttgacgtact cgccgcggga
      961 ctcatccagg ttcttgccca actcctgggc tgttatgaag gtcagttcct cgatgtgctg
     1021 ctcgatgagg ctcttgaagt ggaacagcag ctgggttcgg acggcgatgg gcttgtctgc
     1081 ccaggcaggg aatgccgcgg cggccgcctc caccgcctcg ctcacctccc ctgcggtaca
     1141 tgccggtgtc tcggcgatca gggatccggt ggatgagtcg gtgaccggca tgtactttcc
     1201 gctcctggac tccttccact ctccgttcgc aaagtacttg agcctctttg tctctgccat
     1261 ctttcgctca acccctctcg tgcagtgtca ggccatcctc atcaccaaca accagttatt
     1321 cgccacgtgt caggaaacga gcgcttggca gcgttgtgag gggtgatgcc ggccccatcg
     1381 ccgcccaatg gagcaactgc aaagatgatg ccgagggagg aggtgagata agaaaagggg
     1441 cgccggatcg gcgccccctc ctgactactg atgactgata gctgttggct atccgatggc
     1501 ctggcggtag atcttgacga tcgcgtcgcg cgtcggcttg accgggttga gcgggatgtt
     1561 gccgctcttg aaggtgtctt ccaccatctc gtccaggtgg ctgtagtcca caccgtagtc
     1621 cttgaaggtc tccgggatcc cgatgtcgtg cgacagctcg cgaaccgccg ttacggaggc
     1681 gcgagcggct tccatcgtcg tcatgccgaa ggtgtcgacg ccc
//
LOCUS       DATALS010000038         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_228659, whole genome shotgun sequence.
ACCESSION   DATALS010000038 DATALS010000000
VERSION     DATALS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228659"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2535
                     /locus_tag="VHS06_00760"
     CDS             <1..2535
                     /locus_tag="VHS06_00760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2986697.1"
                     /translation="VTFSLNNYAMSESLDNDTATNRATTFLTALISLPNLHRLQGQPT
                     MSASSRARDLLLSLIAVPGLAVVTLLLLSAQALARIVAPRPAKTGTDAPSAETLSVVV
                     AAGDDGQGMERTLRSVAVAAAAEGWPDHEVIVVGPAKSIDSLKNRETLPDLRLLRSES
                     KELLQNWAAGIEASIGSLVILLVEGAELDEVSLDSLTSEFADGRTFAYAPVWELAGEK
                     AGNVQTGSFRGEVRNGELHFWNASLPEAGRHPSPVLWSSGTGCVLRRDRFLELGGFDP
                     QFGSPDTSFTDLCYRAWKREWPVVQGPDIRVTIHDRQAQLHQQGSPDLNQRDHLIFAW
                     KDLSSPGMLCLTQLALTLRLLSDGVRGQLNLRPIRLALSELPCVLIHRLHRPASPMLS
                     DIAVFDRSSRRSVLQLPVPLDAETGKAIPHNEGSTSALSAILKTDSSPLRMLLVCPQL
                     PYPPSHGAAVRMWNVLKVLAERNRVDVLSFYETHLSKDEVDASVREMERYCGEVRVVP
                     RRPDYRPSLLDRTVHVEQFDCPAMREALREMVERNGYDVIQVDKTEMAQYALPRPAPV
                     QLLIEHIIFYHAYRRQFLTRDRVSLHRLIEYFKLRRFELRACRDFDGIVTMSQVDADF
                     LRSRLPDHRCIVDSPNGVDLSHYRYIHEPAKNHDLLFVGNYDHSPNVDGIRFFLKEIF
                     PVIKAEVPDVGLWIVGPGRYDALPEVAAEPQAVTTGFVDDTWPYMARCSVLVAPILAG
                     SGTRLKILEAMASGIPVVTTAIGIEGIRAKNGVHAMIADDGHGFAQSVVTLLHSREMR
                     ETLRSSARQLMEGRYDWRSIGLRLEDSCRQLMRDRGSSPNRSSDIR"
     gene            2809..3324
                     /locus_tag="VHS06_00765"
                     /pseudo
     CDS             2809..3324
                     /locus_tag="VHS06_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
BASE COUNT          736 a         1012 c         1001 g          656 t
ORIGIN      
        1 gtgacatttt cgctgaacaa ttacgccatg tcagaatcgc tggacaacga cacagccact
       61 aatcgagcta ccactttcct cactgctctg atatcattgc cgaaccttca tcgactgcag
      121 gggcagccca cgatgtcagc ctcctcccgc gcacgagatc tgttgctttc gctcatcgca
      181 gtccccggcc ttgccgtggt gactctcctc ctgctgtccg cgcaagcatt agccaggata
      241 gtcgcgccgc gtcctgccaa gaccggtacg gacgccccaa gcgccgagac attgagtgtg
      301 gtcgtagcgg ctggggatga tgggcagggg atggagcgaa ccctgcgatc ggtagccgta
      361 gccgcggccg ctgaaggctg gccggaccac gaggtgatcg tggtcggccc tgcgaagagc
      421 atcgacagtc tcaagaatcg cgagaccctt cccgatctga ggctgctacg aagcgagagt
      481 aaggaactgc ttcagaattg ggcggcagga atcgaagcat cgatcggcag tctcgtcata
      541 cttctagtcg agggagccga actggatgaa gtgtcgctgg actccctcac ttcagagttc
      601 gcggatggac ggacattcgc ttatgccccg gtttgggaac tggcaggcga aaaggcaggc
      661 aacgtccaga caggtagctt ccgcggagag gtgcgcaatg gcgaactgca tttctggaac
      721 gccagtctac ctgaggctgg ccgccacccc tctccggtcc tatggagttc cgggacaggc
      781 tgcgtgctac gcagggaccg cttcctggaa ctcggaggct tcgacccgca gttcggcagc
      841 cccgacacct cgttcaccga cctgtgctac cgcgcctgga agcgggagtg gcccgtcgtc
      901 caggggccag atatcagggt cacgattcac gacaggcagg ctcaactcca ccaacagggc
      961 tcgcccgacc tcaatcagag ggatcacctc atcttcgcct ggaaggatct ctcatctcca
     1021 ggcatgcttt gcctgaccca gcttgcgctt acactgaggc tcctctcaga cggtgtacgg
     1081 gggcaactga atctacgccc catacgattg gccttgagcg aacttccctg cgtcctgatc
     1141 caccggctgc accgtccggc atcaccgatg ctctcggata tagcggtctt cgaccgctca
     1201 tcgcggaggt cggtactcca gttaccggtc cctctcgacg cggagactgg gaaagcaatc
     1261 ccccataacg agggttcgac ctcggcgctg tctgcaatac tcaagaccga ctcctcgccc
     1321 ctgaggatgc tgctggtgtg tcctcagctc ccctatccgc cgagccacgg tgcggctgtc
     1381 cgaatgtgga acgtgctgaa ggtgttggcc gagcgaaatc gagtagacgt tctatccttc
     1441 tacgagactc acctatcgaa ggacgaggtc gatgcctccg ttcgggaaat ggagcgttac
     1501 tgcggagagg tccgagtcgt gccgcgcagg ccggactatc ggccgagctt gctggatcgt
     1561 accgttcatg tagagcagtt cgactgcccg gcgatgcggg aagcgctgcg cgaaatggtg
     1621 gagcgcaacg ggtacgacgt catccaggta gacaagaccg aaatggcgca gtatgcgctc
     1681 ccgcggccgg ccccggtcca actactgatc gaacacataa tcttctatca cgcctaccgg
     1741 cgccagtttc taacacgcga cagggtctcg ctgcatcgcc taatagagta cttcaagctt
     1801 cgtcgtttcg agctaagggc gtgtcgcgac ttcgacggca tagtcactat gagccaggtg
     1861 gatgccgatt tcctccgatc ccgccttccc gaccaccggt gcatcgtcga cagtcccaac
     1921 ggcgtggacc tatcccacta cagatacatt cacgagccag caaagaacca cgatctactg
     1981 tttgtcggca actacgacca ctcgccaaac gtggatggca ttcgcttctt tctgaaagag
     2041 atcttccctg tcatcaaggc cgaagtgccc gacgtcggac tctggatcgt tggaccagga
     2101 aggtacgacg cgctgccgga agtagccgcc gagcctcagg cagtgaccac cggcttcgtc
     2161 gacgacacct ggccatacat ggcgcggtgc tcggtcctcg tggcgccgat cctcgcggga
     2221 tccgggacac ggttgaagat cctagaggcg atggcatcgg gcataccggt ggtcaccacg
     2281 gcaataggga tcgaggggat ccgggccaag aacggcgtcc acgccatgat cgccgatgac
     2341 gggcacggct tcgctcaaag cgtggtgact ctgcttcata gccgagagat gcgagagacg
     2401 ttgagatcga gcgcgagaca actgatggag ggtcgatacg attggcgctc cataggactg
     2461 cgactagagg acagctgcag acaactaatg agggatcgag gttccagtcc aaacagatcg
     2521 tccgatattc gatgagtccg acggcctcag ggcgcaacga cgaactggac aacaggggcg
     2581 tcaacggcta tcttaccggc atcgatggaa gagcgtagcc ggcccaacct agagacatgc
     2641 acaggctgcc accgtcgaca gtctacttgt gaaaacacct cacgttctca cgaaattgta
     2701 aacagacacg acctgcattc cggctctgtc tcgcaaccga tacgcgagca gagccgctgc
     2761 gaaacgcacc taaactcaaa gtgatataga taccaggagc ggatttctat gggcacttcc
     2821 tggaaagata gcagagcatt ctggaaagac aaaagagcaa tcgtaacagg tggcggaggt
     2881 ttcctcggct ccttcgtcac ttcgaaactg acagagcgag gctcggccca ggtgatcgta
     2941 ccgcggagca gggagtacga tcttagggac ctcgacgcca tccgccgtct ccttgccgat
     3001 acgtcgattc caggggagtc gggcagcgga cgccagccgg tggatgtgat cattcacctg
     3061 gccgcagtgg tcggtggcat cggggcgaat cgagagcacc ctgctgagtt cttctatgac
     3121 aacctgatga tgggggcaca actgctccac gaggcgtatc gtgccggcgt gaagaaattc
     3181 gtggccgtag ggacggtgtg tgcctatccc aagttcactc cggttccctt cagggaggaa
     3241 gatctctgga acgggtaccc cgaggaaacc aatgccccct atggactggc aaagaagatg
     3301 cttctggtgc aatctgaagc ccatcccagt ggctgatccg atccttctga taggtgacga
     3361 aagcggcgtc gagattgggg gtgaaagcca tcaaggcacc ctgtt
//
LOCUS       DATALS010000039         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_363582, whole genome shotgun sequence.
ACCESSION   DATALS010000039 DATALS010000000
VERSION     DATALS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363582"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="VHS06_00770"
     CDS             complement(<1..1403)
                     /locus_tag="VHS06_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX2986698.1"
                     /translation="MDNFSNLMMGFSVALTWQNLAAAFLGAFIGTIVGSMPGVGASAG
                     IAMLVPVSFNYDATTALILMCAVYYGCMYGGTITSVLVNLPGESASVMTCLDGYEMAK
                     KGQAGKALAVAQIGSFIWGTLGVLAIMLIGPPLAEFALSFGPPEYFALMFLGLTCLSA
                     MGEGSTVKALLMVALGLFFACMGTDPLAGAARFTFGNFMFLNGIDFIPAAVGLFAVGE
                     LLIEAETVVAMSFGKYSLREMILTKKDWKASAGAWTRGGTIGFLVGALPGAGATISSF
                     IAYFVERKISKHPETFGTGEIQGVAAPEAANNAATAGAILHLLVLGVPGSGATAVMLG
                     ALMIHGLRPGPLLFTQNPEFVWGLIASMYIGNIVLLIMNMPLVPVFVAILRLPYSVLV
                     PIIITVSMIGVYSVNNSMFDVVAMFVFGILGYGFKKFQFPVAPLVLSLVLGEMLERSL
                     RQALAMSHNDPSIFVTRP"
     gene            complement(1428..1889)
                     /locus_tag="VHS06_00775"
     CDS             complement(1428..1889)
                     /locus_tag="VHS06_00775"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX2986699.1"
                     /translation="MRKGDLVISAFLFLMGWWVLWQATMLPHLDLAGPGPEFVPNLVG
                     GMLVVLAGIMFVNTWRGSAVTPEGFIPDRAGIIRVVTMVVGLFFYVTLLEVVGYLVLT
                     FIYTFYTITAMSKYRWYTRAIVAAVITVTFYQGFVGFLEVPLPRGSIFGGS"
     gene            complement(1955..>2975)
                     /locus_tag="VHS06_00780"
     CDS             complement(1955..>2975)
                     /locus_tag="VHS06_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX2986700.1"
                     /translation="APKAAEPTKPAAAAPTTAPAAAAPTAAPKAAEPAKAAAPAWTPE
                     KNVSFIVHAAAGGGSDIQARTMEKVISDEKLYPRNVAVENKVGGSGAVAFAYVASKKG
                     DPYTWLTATTSFFTTPARGQVPLDPVKDFTMLARLSDDFFFVFVKADSPFKTIQDVID
                     AAKKSPDTVKWGGTNVGSDDHIFYSKFAKATGTKFNFIPSPGGGDVVTAVLGGHVDVA
                     SGNPGEVMAQVQAGKLRALASSGDKPLPNYQQIPTLKSLNIDVLYQQTRMIAAPAGLP
                     AGVREYYAGMFKKMVASKGWKDYIQKNDQNENWMEGDTLDKYVKDINESIKALIVELG
                     LVEKK"
BASE COUNT          579 a          941 c          944 g          511 t
ORIGIN      
        1 ggcctggtga cgaagatgga ggggtcgttg tgggacatcg ccagcgcctg gcgcagcgat
       61 cgctccaaca tctcgcccag taccagcgac agcaccaatg gcgccaccgg gaactggaac
      121 ttcttgaagc catagccgag gatcccgaag acgaacatcg caaccacgtc gaacatgctg
      181 ttgttgacgc tgtagacgcc gatcatggag acggtgatga tgatcggcac gagcaccgag
      241 tagggaagcc gcaaaatggc aacgaagacg ggcaccagcg gcatgttcat gatcagcaac
      301 acgatgttgc cgatgtacat gctggcgatc aacccccaga cgaattccgg attctgggtg
      361 aacagcagcg gtccagggcg aagcccgtgg atcatcaacg cgcccagcat gacagcggtt
      421 gcgccggagc cgggcacgcc cagcaccagc aagtggagaa ttgccccggc cgtcgccgcg
      481 ttgttggcag cctcgggggc cgccaccccc tggatctcgc ccgtgccgaa ggtctcgggg
      541 tgcttggaga tctttcgctc gacgaagtag gcgatgaagg aggagatggt ggccccagcg
      601 ccgggcagcg caccgaccag gaagccgatg gtcccgcccc tggtccacgc gcccgccgaa
      661 gccttccagt ccttcttcgt caggatcatc tcccgtagtg agtacttccc gaagctcatc
      721 gccaccacgg tctcggcctc gatcagcaac tcgcccacag cgaacagccc caccgccgcc
      781 gggatgaagt cgatgccgtt caggaacatg aagttgccga aggtgaagcg ggccgccccc
      841 gccagcgggt cggtccccat gcaggcgaag aagaggccga gcgccaccat taggagcgcc
      901 ttcaccgtcg agccctcgcc catggcggag aggcaggtaa gccccaggaa catcagcgcg
      961 aagtactccg gcgggccgaa ggagagggcg aactccgcca gcgggggccc gatcagcatg
     1021 atggccagca cgccgagagt accccatatg aaggagccga tttgggccac cgcgagtgcc
     1081 ttgccggcct ggcccttctt ggccatctca tacccatcca gacaggtcat gaccgacgcc
     1141 gactcgcccg gaaggttgac cagcacggag gtgatcgtgc cgccgtacat gcagccatag
     1201 tagacggcgc acatcaggat gagcgccgtc gtggcgtcgt agttgaagct cactggcacc
     1261 agcatggcga tgccggcact ggcaccgaca cccggcatag accccacgat ggtgccgatg
     1321 aaggctccca ggaaggcggc cgccaggttc tgccaggtga gggccacaga gaagcccatc
     1381 atcaggttgg agaagttgtc caaggatcta ttgctccctt cccgaggcta tgagccgccg
     1441 aagatactac ccctgggcag cggcacttcc agaaagccga caaagccctg gtagaatgtg
     1501 acggtgatga ccgcggccac gatggcccgc gtgtaccacc ggtatttcga catggccgta
     1561 atcgtgtaga aggtgtagat gaaggtgagg accagatagc ctacaacttc caacagcgta
     1621 acgtagaaga agagccccac caccatagtt acgacgcgga tgattccggc cctatcgggg
     1681 atgaaccctt caggggtgac ggccgagccg cgccaagtat tgacgaacat gattccagcc
     1741 agcactacca gcatgccccc caccaggttc ggcacgaact ctggcccggg gccggcgagg
     1801 tccaggtgag gtagcattgt ggcctgccac aacacccacc accccatcag aaacaggaac
     1861 gccgagatca ccaggtcgcc ttttcgcatt gagcgtccct cctagaatag actcgggggg
     1921 ggggcgcaag tcgccccccg agccacccgg ttccctactt cttctcgacc agccccagtt
     1981 cgacgatgag agccttgatg ctctcgttga tgtccttgac gtacttgtcg agggtgtcgc
     2041 cctccatcca gttctcgttc tggtcgttct tctggatgta gtccttccag cccttgctgg
     2101 caaccatctt cttgaacatg ccggcgtagt actcgcggac accggcgggc aggccggccg
     2161 gggcggcgat cattcgggtc tgctgataca ggacgtcgat gttcaggctc tttagggtcg
     2221 ggatctgctg gtagttgggc agcggcttgt cgccggagga ggccagggcg cgcagcttgc
     2281 ccgcctgcac ctgggccatc acctcgccgg ggttgccgct ggccacatcc acgtggccgc
     2341 ccaggaccgc ggtcaccacg tcccctccac ctgggctggg gatgaagttg aacttcgtcc
     2401 cggtcgcctt ggcgaacttg ctgtagaaga tgtggtcgtc ggatccgacg ttggtcccgc
     2461 cccatttaac cgtgtcgggg ctcttcttgg ccgcgtcgat gacgtcctgg atcgtcttga
     2521 agggcgagtc ggccttcacg aagacgaaga agaagtcatc ggagagccga gccagcatcg
     2581 tgaagtcctt caccggatcc agcggaacct ggccgcgagc cggcgtggtg aagaaggagg
     2641 tggtggccgt caaccaggtg taggggtcgc ccttcttgct tgccacgtag gcgaaggcca
     2701 cggcgccact accacccacc ttgttctcca cggcgacgtt gcgggggtac agcttctcgt
     2761 cgctgatcac cttctccatg gtgcgggcct gaatgtcgct accgccgccg gcggcagcgt
     2821 gcacgatgaa gctcacgttc ttttcggggg tccaggccgg cgccgcggcc ttggccggct
     2881 cggcagcctt gggcgctgca gtcggtgcag cagcagcggg ggcagtagtg ggggcggcag
     2941 cagccggctt ggtcggctcg gcagctttgg gagcc
//
LOCUS       DATALS010000040         4809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_183776, whole genome shotgun sequence.
ACCESSION   DATALS010000040 DATALS010000000
VERSION     DATALS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183776"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            599..922
                     /locus_tag="VHS06_00785"
     CDS             599..922
                     /locus_tag="VHS06_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010481247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2958 domain-containing protein"
                     /protein_id="HEX2986701.1"
                     /translation="MRLLTKEIMRKLPALYATEDVPDGHRMALVKFFNPTSGGTWYVF
                     EGVAQEDGDWLLFCYVVDLGFDELGYVSLRELEGVRLPSGLGIERDLHFRPVSLAALL
                     AGQRP"
     gene            1257..1595
                     /locus_tag="VHS06_00790"
     CDS             1257..1595
                     /locus_tag="VHS06_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986702.1"
                     /translation="MEHGAVLSGANDKKHMMMAHIKAVADSIVSDQRGDTVFERYFRQ
                     VRHAHRYSMRNLMLIDWQAPDSRFVASLSAFEEIAKEQAVPAVDLGGRLRRWNSRKRP
                     DLHMPRMQRR"
     gene            1747..2184
                     /locus_tag="VHS06_00795"
     CDS             1747..2184
                     /locus_tag="VHS06_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986703.1"
                     /translation="MELQAIHEQYSPRIQALLEGLASALRHAGYTVAGPDDSSGYEDF
                     VWRMLVTTGERQEPQDDDIDVHFWICDSESWAGDNGGISVLLDLVTFGGRLVGQFCPM
                     NYTPKVWVPREDPDAIEERFVSMEHLDIDSTVALVQEYLPLAA"
     gene            2806..2973
                     /locus_tag="VHS06_00800"
     CDS             2806..2973
                     /locus_tag="VHS06_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986704.1"
                     /translation="MDWLREAVERANELRPTKLAVRAKHCLLRLGTRGQFDGAIYELD
                     MITATDPELSE"
     gene            complement(3119..4180)
                     /locus_tag="VHS06_00805"
     CDS             complement(3119..4180)
                     /locus_tag="VHS06_00805"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2986705.1"
                     /translation="MTDCADFACLLKEFQGIPRSPRSAPTFMEISGYPHLENVCSNIL
                     RFFLDADECHGMGDLLLRALFLGLGRDVEGVGFRDVTVERECSTDRGDRIDLVVETDR
                     YLIGIENKLFHGIANDLDHYAGHLRRRAADIGGKEVILILLTLLPVDETVCGPFQRIT
                     YERWFAQVDDILGDHAQGCDQRYLLYLLDFVHTVRGLVKRPEMNPELLVFLADNWETI
                     RDLNNQVRTLRDELKRQQEDVAALVQLDESSGIKPWTWGGDTDWPWPSFGYDIPFLGD
                     GKIVVDAFLTSCWVIEIHTRQKSPEEFRRWLAERGIHTQREQDGRLICAEFSYGTPLI
                     KVAECLSGLLVTLSSEDGV"
     gene            complement(4290..>4809)
                     /locus_tag="VHS06_00810"
     CDS             complement(4290..>4809)
                     /locus_tag="VHS06_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986706.1"
                     /translation="HDVAYEVRSDSERIPGGNLRDYLLTAMYRSVAPDALDKTRFGAA
                     DIRHFLRHGMQWNRFACCAFDCGPILVGKARHIAYDELAAARRTFFDGCNVNGATLQA
                     KRTLVRAALEPLVDFLALYYEEPFSLVQRFRGFREHRDALRTMIHDTQRVLNDLIERE
                     QLDLPVLTYLPE"
BASE COUNT          996 a         1370 c         1485 g          958 t
ORIGIN      
        1 gttatgggag tacgcgcatg gaaggtgacg aatatggagc cgaagcagat ggactggtta
       61 cgagaggcgg tagagcgggc gaacgaactc aggccgacga agctcgccgt ccgcgccaag
      121 cactgtctcc tccggcttgc ccctcctaaa ataacaaacg agtgtgatca acacagtgtt
      181 tcacctgata ccacaagttc atcaccaggg ctgagaaggc caaccggatc ctctccacgc
      241 agcccatccc acatcggtgg tgaaataccc acgttttcta ctggagatcc ctcgcaggaa
      301 ctctactggt aggccaaccg ttaccgaagc gcctttctgt tcgggcacgt ggtgagtgtt
      361 gggcgtagat aacggcaatt ctgggggtgc cgcacggact gcccccagat ttcccgacag
      421 gatagcacct gggacgatga cgaggacgat gcgggatcgg cggttacctt tactgcgtat
      481 cctgcgggca ggcgacgacg cttccccctg gcgtggcggt cgagggggag cggcttcagc
      541 aagcgcggtg aaacaaccct gcggtgccac tcgtttattg acacggagga tgatgaacat
      601 gcgactgctc accaaggaga tcatgcggaa gctcccggcc ctctatgcca ccgaggatgt
      661 gcccgatggc cacaggatgg ccttggtgaa gttcttcaac ccgaccagcg ggggtacctg
      721 gtacgtcttc gagggcgtgg cacaggaaga tggcgactgg ctgctcttct gttacgttgt
      781 ggatctgggc tttgacgaac tgggctacgt gagcctgcgc gaattggagg gcgtgcgttt
      841 gccctcggga ctgggtatcg agcgcgacct acacttcagg ccggtctccc tcgcggcgct
      901 actggccgga cagcgtccgt agggggcatc gctggggccg agggtgtttc acctcgaccc
      961 cgcaattgtt catccgtatg gaaggcaatg gtaggggtca tctacggagg gcatatgaca
     1021 cagaacaagc tcctgttcga gcgtgccggg gagaatagcg gcacggcccg gacgccggtc
     1081 ggtgtttggc gtgggcgcag gccgaggagc aggtcgtggt tgctgcccga tactgcaggc
     1141 gctgctggga gttccggctc ccggcagcgc atttccgcgt tgatgcaaca tgctaacacc
     1201 ttggtgaaac acatattccc tgccactcgt ttatgtatct ggaaggagaa agacgcatgg
     1261 aacacggagc ggttctcagt ggtgctaacg acaagaagca tatgatgatg gcccacatca
     1321 aggcggtcgc cgactccatc gtgagcgacc aacgcggcga tacagtgttt gagcggtatt
     1381 tccgtcaggt ccggcacgcc caccgctact cgatgcgcaa tctcatgctc atcgactggc
     1441 aggctccgga ctcccggttc gtcgcgtcgc tttctgcgtt cgaggagatc gcaaaggagc
     1501 aggccgttcc tgctgtcgat cttggtggtc ggttacggcg ctggaactcg aggaaaaggc
     1561 ctgatcttca catgcctcgc atgcagcgaa gatgatggcg agcccgatat tttccacatt
     1621 gtcgcgggcc atacctggga agatgtggaa ttagtgctcg gtatcgaaat tatctgaatg
     1681 ttggatgtta cggatgagac aacttcacat agcgcctact cgctaatatg tttaaaggga
     1741 gatacgatgg agcttcaagc aatccacgag cagtattcgc cgcgcatcca ggcactactc
     1801 gaaggtctag catcagcact acggcatgca ggatacacgg tggctgggcc ggacgactca
     1861 tccggctacg aggatttcgt gtggagaatg ctggtgacga ccggcgagcg gcaggaaccc
     1921 caggatgatg acatcgacgt gcatttctgg atctgtgata gcgaaagctg ggcgggcgac
     1981 aatggcggca tcagtgtcct gctcgatctg gtgacgttcg gcggcaggct ggtggggcag
     2041 ttctgcccga tgaactacac gcccaaggta tgggtacccc gcgaggaccc agacgccatc
     2101 gaggagcgat tcgtctcgat ggaacacctt gacatagata gtactgtggc gctggttcag
     2161 gagtacctgc cgctcgcggc gtaggcccag ccgcatgacc caaacgggtg ccgtcgatgg
     2221 gtccggtgtc ccgattgaga ggaatgtgac aacatgatga gcgaaggcga gagggatcgg
     2281 ctgctgcgtg acgtacacgc aagcgcgaga gtgcacttcg gtacctgtgc tctctatacc
     2341 agctagtccg gcgtggtcat cgagaaggta ggattcggtg acgttctcct cgtctacgcg
     2401 atgatcgcgt gtcgatacga ggacacatgt gccctcgtgc ccgtcgcacg ccagtacgtg
     2461 ttcgggtgac tgcagccaca gggggtgccc aatgatgttg tggagttcgt gccaggcata
     2521 gtacgagaga aggagcgtta ccgtttcgtt gatacgaatg aggccagcgg gtcctttagg
     2581 aagggcagga ttggctgtct gcagcagttc ggcttcagga tcaggtccga aggggtgggc
     2641 ttcttcgagt ggcgggggtg acagggcaag gtgaagccag acccgttggg catcggccag
     2701 gcacggctcg tgtagtgatg atgaggtgaa acagaccagg cggcgatctc ttttatggtt
     2761 atgggagtac gcgcatggaa ggtgacgaat atggagccga agcagatgga ctggttacga
     2821 gaggcggtag agcgggcgaa cgaactcagg ccgacgaagc tcgccgtccg cgccaagcac
     2881 tgtctcctcc ggctaggcac gcgggggcag ttcgatgggg cgatctacga attggacatg
     2941 atcaccgcta ccgatccgga attgagcgag tgactcgatg acaacgggca tccaggcagg
     3001 gcctaccacc cacaacctgt ggactccgcc caagcgctct gtcgctcggt tgtgcgatgc
     3061 gctcgccggg tggaagatgg gcggtacgga aaccgcctgg atccgtccgg cggcgcactc
     3121 agacaccgtc ttctgaggag agtgtcacca acaagccgct caggcactcc gccaccttga
     3181 tgagaggcgt gccgtacgag aactccgcgc agatgaggcg accgtcctgc tcccgctgcg
     3241 tatggatgcc acgttctgcc agccaccgtc tgaattcttc cgggctcttt tggcgtgtgt
     3301 ggatctcgat gacccaacat gacgtcagga acgcatcgac gacgatcttc ccatctccga
     3361 ggaatgggat gtcgtagccg aagctgggcc agggccagtc tgtatcaccg ccccacgtcc
     3421 acggtttaat accgctgctc tcatccagtt gcaccagcgc tgcgacatcc tcttgctgcc
     3481 tcttcaactc atcacgcagt gttctcacct ggttgttcag gtcgcggatg gtctcccagt
     3541 tatcggccag gaatacgaga agctcagggt tcatctccgg tctcttcacc aatcctctca
     3601 cagtgtgcac gaaatcaaga aggtagagaa ggtaacgctg atcacagccc tgggcgtggt
     3661 ccccgaggat gtcatccacc tgcgcaaacc agcgttcgta ggtgatacgc tggaaaggcc
     3721 cacacaccgt ctcgtcaacc ggcagcaagg tcaaaaggat aagaatcacc tctttgccac
     3781 cgatgtccgc cgctctccgt ctgaggtgcc cggcgtagtg gtcgaggtca ttggcgatgc
     3841 cgtggaacag cttgttctca atgccaatga ggtacctgtc cgtctcgacc accaggtcga
     3901 ttctgtcgcc tcggtcggtg gaacactccc tctctaccgt cacatcccgg aagcccacgc
     3961 cctcgacatc ccggccgagg ccgaggaaga gcgccctcag cagaaggtct cccatcccgt
     4021 ggcactcatc cgcatccagg aagaagcgca ggatgttgct gcacacgttc tccagatgcg
     4081 gatagcccga gatctccatg aaggtggggg cgctccttgg cgagcgaggg atcccctgga
     4141 attcctttag taggcatgcg aagtcagcgc aatctgtcat gttcgagatc cttgtaccag
     4201 ccgcgacggg tgataatggc aatggccccg cgcgtctgca acggcatcgg cttctacagc
     4261 agcttccctg cctcatagcc acaccacacc tattcaggca ggtacgtcag cactggcaga
     4321 tcgagctgct cgcgctcaat gaggtcgttg agcacccgtt gggtgtcgtg tatcatggtc
     4381 cgaagcgcgt ctcgatgttc ccggaaccct cggaagcgct ggaccagcga gaacggctcc
     4441 tcgtaataca gcgcaaggaa gtctaccagc ggctcaagcg ccgctctcac aagcgtacgc
     4501 ttcgcctgca acgtcgcacc gttcacattg caaccatcga agaaagtccg ccgtgcagcg
     4561 gcaagctcgt cgtaggcgat atgcctcgct ttccccacca gtatcggccc gcaatcgaaa
     4621 gcgcaacagg cgaaacggtt ccactgcatg ccgtgccgca ggaagtgccg gatgtcggct
     4681 gcaccgaagc gcgttttatc cagggcatcg ggagccacgc tgcggtacat cgccgtgagc
     4741 aggtagtcgc gaagattgcc gccaggaatc cgctcactgt cggaacgcac ctcgtaggcc
     4801 acgtcgtgg
//
LOCUS       DATALS010000041         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_138509, whole genome shotgun sequence.
ACCESSION   DATALS010000041 DATALS010000000
VERSION     DATALS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138509"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1188)
                     /gene="ispH"
                     /locus_tag="VHS06_00815"
     CDS             complement(301..1188)
                     /gene="ispH"
                     /locus_tag="VHS06_00815"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX2986707.1"
                     /translation="MEIVRAKHHAGFCGGVKRAWRLATSEAETATDPVYISGKLIHNT
                     PAMKELEEKGIHVLELSNAEGVQAGTTFILRAHGEGPAVYARARELGLRLIDGTCPTV
                     RAVQRLARSLEEQGFQVVLFGHREHPEAKATIAHTQRGLIIQSAEEAAQLGRYAKIAS
                     IAQTTSSSREYAEVCRILKDKCDEFQDHGHICNFTQKAQEEAAELASRVDTMVVVGGR
                     DSSNTRRLVEVCAERCATYQVETAEELQPDWFQGVARVGVAAGASTRDGDIEAVISRL
                     GDLGAGAGAGGQGSGAGER"
     gene            1273..2466
                     /gene="dxs"
                     /locus_tag="VHS06_00820"
     CDS             1273..2466
                     /gene="dxs"
                     /locus_tag="VHS06_00820"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX2986708.1"
                     /translation="MPDYPILSRIDNPRQLAALNSQQLRQLAEEIRQFLLEEVPITGG
                     HLSSNLGVVELTIALHRVFESPRDKIVWDVGHQGYVHKLLTGRRDSFRTQRQFGGLSG
                     FLVREESVHDHFGAGHAGTSISAALGMAVARDLKHEDHHVVAVIGDGSLTCGMALEAM
                     NHAGHLGSRLIVVLNDNEMSIAHNVGAISRMLNEVRVHPRYHKAKEEMDHVLQRLPGG
                     GSATDAGRKVLHGLKALVLPNILWEELGFTFVGAVDGHNIAELELALQRAKSYTKPAL
                     IHVMTQKGHGYGPAEEDSTRWHGVAPNGAKKRNAPTYTEVFGKTLLREMHDNSRVVAI
                     TAAMPDGTGLAPAAKEMPSRVFDVGISEQHAVTFAAGLACQGLVPVVAIYSTFLQRSS
                     GFTPW"
BASE COUNT          422 a          810 c          769 g          487 t
ORIGIN      
        1 ccccctgccc ccttctccct tgcccaggga gaagggggag gagatagtcc ttctggggac
       61 accccagacc ccgaccgaag gggcctgcgg ccccctctgc actcccccgc tgaaggtgag
      121 aagaccgccc gccctggatc cgctcggagg gggctgcagc cccctctgca ctccccaact
      181 ggaggtgaaa agaccccgcc ctggatccgc tcggaggggg ctgcggcccc ctctgcactc
      241 cccaactgga ggtgaaaaga cgccgcgctg gacccgctcg gaggggcatg cggataccta
      301 ttaccgctcc ccggcccctg acccctgacc cccggcccct gcgccggcac ctaagtcgcc
      361 gaggcgactg atgacggcct cgatgtcgcc atcgcgcgtg gaggcgcccg cggcaactcc
      421 gacccttgcc acaccctgga accagtcggg ctgaagctct tcggccgttt ccacctggta
      481 ggtggcgcag cgctcggcgc acacttccac cagtctccgg gtgttggagc tgtcccgtcc
      541 accgactaca accatggtgt ccaccctgct ggccaactcc gctgcctctt cctgtgcctt
      601 ctgcgtgaag ttgcagatgt ggccgtggtc ctggaactcg tcgcacttat ccttgaggat
      661 gcgacacact tctgcgtact cgcgggagga ggaggtggtc tgggcgatgg aggcgatctt
      721 ggcatagcgt cccagttgag ccgcctcctc ggccgattga attatcagcc cgcgttgagt
      781 gtgggctatg gttgctttgg cttcgggatg ctccctgtgg ccgaatagca ccacctggaa
      841 cccctgctct tcaaggctgc gcgccagtcg ctgcactgct cggacggtcg gacatgttcc
      901 gtctatcagc ctgaggccca gctctcgggc acgggcatag actgccggac cctctccgtg
      961 tgccctgaga atgaaggtgg taccggcctg taccccctcg gcattcgaca gctccagaac
     1021 gtggatccct ttctcctcca gttccttcat ggcgggagtg ttgtggatca gcttgcccga
     1081 tatgtacacg gggtccgttg cggtctcggc ctcgctggtt gcgagccgcc atgcccgttt
     1141 cacgcccccg cagaagccgg cgtgatgctt ggctcttacg atctccacct tgccctccgt
     1201 tctcgccaat gtagattcta tcgcccgttt ctgttagcat caacctgttt tcctgttggg
     1261 aggctgtcga ttttgcctga ctacccgata ctgagccgta tagataatcc gcgacaactg
     1321 gctgcactta actcgcagca actgaggcag ctggccgagg agataaggca gtttttgctg
     1381 gaagaagtcc cgataaccgg cgggcaccta tcctccaacc tgggggttgt ggagcttacc
     1441 atcgccctgc atcgagtctt cgagagcccc agagacaaga ttgtgtggga cgtggggcac
     1501 cagggctacg tccacaagct gctcaccgga aggcgggata gcttccgaac ccagaggcag
     1561 ttcggcgggt tgtcgggctt tctagtgcgg gaggagagcg ttcacgacca tttcggcgcc
     1621 ggccatgccg gcacatccat ttcggcagca ctcgggatgg ccgtggcgcg cgacctgaag
     1681 catgaggacc accacgtcgt ggcggtgatc ggcgacggct ccctcacctg cggtatggcg
     1741 ctggaggcga tgaaccatgc cggccacctg ggtagccggc tgatcgtggt actgaacgac
     1801 aacgagatgt ccatcgccca caacgtgggc gcgatttctc gtatgttgaa cgaggttcgg
     1861 gttcatccgc gctaccacaa ggcgaaggag gagatggacc acgtcctcca acggctgccc
     1921 ggtggcggct ccgctaccga tgccggccgc aaggtgctgc acggcctgaa ggcgctggtg
     1981 ctcccgaata tactttggga ggagctgggc ttcaccttcg tgggggccgt ggatggccac
     2041 aacatcgcgg agttggaact ggcgttgcag cgcgccaagt cgtacaccaa gcctgcgctg
     2101 atccacgtga tgacccagaa ggggcacggt tacggccctg ccgaagagga ttccaccagg
     2161 tggcatggtg tggcgcccaa cggagccaag aagcggaacg ccccgaccta caccgaggtc
     2221 ttcggcaaga cgctactgcg ggagatgcac gacaactccc gggtggtggc gatcacggcg
     2281 gcgatgcctg atggtacggg cctggctccc gctgccaagg agatgccttc ccgggtgttc
     2341 gacgttggca tttccgagca gcatgcagtt accttcgctg caggcctcgc ctgccagggg
     2401 ctcgtgccgg ttgtggctat ctactcgacc ttcctgcagc gctcctcggg gttcaccccg
     2461 tggtagccga agaataccat cccctgca
//
LOCUS       DATALS010000042         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_407286, whole genome shotgun sequence.
ACCESSION   DATALS010000042 DATALS010000000
VERSION     DATALS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407286"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2004)
                     /locus_tag="VHS06_00825"
     CDS             complement(<1..>2004)
                     /locus_tag="VHS06_00825"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX2986709.1"
                     /translation="IDPANDYAIGVRPLLEDRAVVCEQRTWHGPYSREQGRQLNAAEE
                     RKIPYEDIYPTQWPAVSSSRDRLVEEMGVGEADRAVQAALNRKQPQVRFERMAFEDVI
                     DLLRDTTGLDIQVDWKQLQQAGIDPKAPVQARMRDVKLATLLDEVLRQVSTNPANKLV
                     YTVDEDGITVSTVAGARSPKPSPFVTRANEELWIIGRVSSGEPRQSDDTPGSGQLAAK
                     QTKDGKEQLIPVPLKHTDVKASIAAYISSTTVRQQFHNPYAQNIEAVYVFPLPENAAI
                     NEFVMTVGQRKIRGIIREREEAEQLYQEARSQGHVASLLTQERPNVFTQSVANIEPGK
                     QIDIEITYYHTLAYTDGWYEWAFPMVVGPRYNPANSTQGVGAAPRGKPGITGQATEVQ
                     YLKPAERSGHDIGLSIELNCPAGIEEIRCTSHRINVDHSAPGRATITIASSDTLPNKD
                     FVLRYRVAGGQIKSGMLVHRDQRGSYFNLMVYPPMELRQQDRTPLEMVFLLDVSGSMS
                     GDPINQSKAAMRYALSHMSPGDTFQVITFSGSNRTLFDRAQPVTREATDRVMHFVEDS
                     SAGGGTEMMPAITAALSAAQDPQHKRVVVFLTDGFVGNEDDILRAVHDRLGSARIFSF
                     GVGSSPNRYLMDGLARIGHGTVAYLGLRDSGEDVMRLYFDRVA"
BASE COUNT          316 a          547 c          719 g          422 t
ORIGIN      
        1 caggcaacgc ggtcgaagta cagacgcatg acatcttcgc cgctgtcgcg cagacccagg
       61 taggcaactg tgccatgccc gatgcgggcc aggccgtcca tcaggtagcg gttgggcgaa
      121 gagcctacgc cgaaggagaa gatgcgggcc gagccgaggc gatcgtgaac ggcgcggagg
      181 atgtcgtctt cgttgccgac gaagccgtcg gtgaggaaga cgacgacgcg tttgtgctgc
      241 gggtcttgcg cagccgagag agcagccgtg atggcgggca tcatctctgt gccgccgccg
      301 gcgctgctgt cttcgacgaa atgcatgacg cggtcggtgg cttccctggt gacgggctgg
      361 gcgcgatcga agagggtacg gttgctgccg gagaatgtga tcacctggaa ggtatcaccg
      421 ggactcatgt gggagagggc gtagcgcatg gcggccttgc tctggttgat cggatcgccg
      481 ctcatggagc cggagacatc gaggaggaag accatctcca gtggggtgcg gtcctgctgg
      541 cggagttcca tcggtgggta gaccatcagg ttgaagtagg agcctctctg gtcacggtgc
      601 acgagcatac cggacttgat ctggccgccg gcgacgcggt agcgaaggac gaagtccttg
      661 ttgggcagcg tgtcgctgga tgcgatggtg atggttgccc ggccgggtgc ggaatggtcg
      721 acgttgatcc tgtgcgaggt gcagcggatc tcctcgatgc cggcggggca gttgagttcg
      781 atggagaggc cgatgtcgtg gccggagcgc tcggcgggct tgagatactg gacctcggtt
      841 gcctggccgg tgatgccggg tttgccgcgt ggggcggcac cgacgccctg agtggagttg
      901 gcggggttgt agcgtgggcc gacgaccatc gggaaggccc actcgtacca gccgtcggta
      961 taggcgaggg tgtggtagta ggtgatctcg atgtcgatct gtttgccggg ttcgatgttg
     1021 gcgaccgact gggtgaagac gttgggacgt tcctgggtga gcaggctggc gacgtggccc
     1081 tggctgcggg cctcctggta gagctgctcg gcttcttcgc gttcgcggat aatgccgcgg
     1141 atctttcgct ggccgacggt catgacgaac tcgttgatgg ccgcgttctc aggcagtggg
     1201 aagacgtaga cggcttcgat gttctgggcg taaggattgt ggaactgctg gcggacggtg
     1261 gtgctagaga tgtaggcggc gatgctggct ttgacgtccg tgtgcttgag ggggacgggg
     1321 atgagttgct ctttgccgtc ctttgtctgt ttggcagcaa gctggccgga accgggggta
     1381 tcgtccgact gccgtggctc gccagagctg actcggccga tgatccagag ttcctcgttg
     1441 gcacgggtga cgaatggcga tggcttaggg cttctggcac cggctacagt ggagaccgtg
     1501 ataccgtcct cgtctacggt atagaccagc ttgtttgccg gattggtgct gacctgacgg
     1561 aggacctcgt ccagcagggt cgcgagcttc acatcacgca tgcgagcctg taccggcgcc
     1621 ttggggtcga tgccagcctg ctggagctgt ttccagtcca cctggatgtc caggccggtc
     1681 gtatcacgca acagatcgat gacgtcctcg aacgccatac gctcgaaccg cacctgcggc
     1741 tgcttccggt tcagtgccgc ctgtaccgcc cgatccgctt cacctacacc catctcttcc
     1801 accagccggt cgcgactgct gctgacggct ggccactgtg ttgggtagat gtcctcgtag
     1861 ggaatcttcc tctcttctgc agcgttgagt tgtctgccct gttcgcgact atacggccca
     1921 tgccacgtcc gctgttcaca aacgaccgcc cggtcctcca gcagcggccg gacgcctatg
     1981 gcgtagtcgt tggcggggtc gatc
//
LOCUS       DATALS010000043         5675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_228780, whole genome shotgun sequence.
ACCESSION   DATALS010000043 DATALS010000000
VERSION     DATALS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228780"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VHS06_00830"
     CDS             <1..796
                     /locus_tag="VHS06_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986710.1"
                     /translation="PAPVVRKLVVQSGFGDWGSGHQIHFTDLLVSHYANPHGLDASGH
                     YSTARDLATLGAYAMQNPEFAKIVGTAKISFSWPGHDVKREMANHNKLLGVVPYVNGI
                     KTGYTGKAGYCLVASGSQNGVNLVSVIMGEETNPQCSADTKALLDYGFSLYKKQTLVP
                     SGAVLAEIPIPYRIGESLKLVAEKPLIRTLHLSEVVSKNIEYPELAPVPIEQGQVLGR
                     LVCTVGNKEIGEVNLVAAAPITKPTLNDKLSYYWSRFSGWLQSLVS"
     gene            800..1747
                     /locus_tag="VHS06_00835"
     CDS             800..1747
                     /locus_tag="VHS06_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEX2986711.1"
                     /translation="MILTVTLNPALDRTLAVPNFDIGFRHRATETVTLPGGKGINVAR
                     VVKGLGQPVIATGFVGGRTGERVLSDLEREDVLCDFVRIGGETRTSTAVLDPTAGTTT
                     EVNEYGPQIQKHELELLFEKLDYLSRAASIVVLAGSIPRKVDTNIYAQLIGKVKRPDL
                     TIIVDTYGEPLRHAVKSGPDLIFPNQFEAETLIGHEFGTEEEFISATAGLRKMGACSA
                     VLHGRDLCVAQVAKNGQVTTLVTRPPAVDVVSTVGSGDSLVGGYAAYMVEGHPPVECL
                     RFGMGCAVANTLSYGAGVFSAEEARRFAEIIEVKESMLA"
     gene            1744..3291
                     /gene="guaA"
                     /locus_tag="VHS06_00840"
     CDS             1744..3291
                     /gene="guaA"
                     /locus_tag="VHS06_00840"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX2986712.1"
                     /translation="MKGRYEELHRVLVLDFGAQYAQLIARRVRECRVYSELIPYNTPV
                     EEIRALKPAGLILSGGPRSVNEPGAPKVDPAILDLGVPILGICYGLQLLVEMGGGKVG
                     KAELSEYGGTSFFVVDETEMFKGLPAEQTCWMSHGDSVLEVPAGYRVTARSQHLPVAA
                     CEDAERELYGVQFHPEVRHTRFGLEILKNFLYRVCNLAPTWTPVSIIEDQMERIREQV
                     GNNKVICGLSGGVDSAVAALLVYRAVGDQLTCIFVDHGLMRKGEAEEVENAFRNHFHV
                     PLVHVKAQRRFLEKLRGVTEPEAKRKIIGNEFIRTFEEEAAKIGDCPFLVQGTLYPDV
                     IESGTSTAAVIKSHHNVGGLPEDMAFQLVEPLRMLFKDEVRAVGEELGLPETMVWRQP
                     FPGPGLAIRIIGDVTEERLHLLREADAILLEEIRRAGLYREIWQCFAVLPAIRSVGVM
                     GDERTYAYPIIIRAVTSDDAMTADWARLPYDVLERISTRIINEVKGINRVALDISSKP
                     PSTIEWE"
     gene            3429..3884
                     /gene="rsfS"
                     /locus_tag="VHS06_00845"
     CDS             3429..3884
                     /gene="rsfS"
                     /locus_tag="VHS06_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX2986713.1"
                     /translation="MIEPAAGAPSEPRERIRQPLTEEQHEEIRRRARKAAEIAADLKA
                     EDIRILDMRSLVTYTDYLVICTGRSTRQTRRISEEIGFRLKKEFGLLPLGVEGDAAGE
                     WILMDYLDFIVHVFTPETREFYRLDVMWKQAPTEVLAPGSETGAEATEG"
     gene            4073..4145
                     /locus_tag="VHS06_00850"
     tRNA            4073..4145
                     /locus_tag="VHS06_00850"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4106..4108,aa:Ala,seq:ggc)
     gene            complement(4298..4777)
                     /locus_tag="VHS06_00855"
     CDS             complement(4298..4777)
                     /locus_tag="VHS06_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986714.1"
                     /translation="MMEKGVDPGRRTETFVEIGLELTTERWREVPFFLRAGKALSRRR
                     KGVVIHFRPGGGQVANELWIGIDGPRDIVLRLAGRAAGRPVPIDLAGEPPRPLLPAYA
                     RVLLDVLSGGNVLSVRADEAEEAWRVVTPVLEAWHEDRVPLQEYPAGSDGPPRESLQ"
     gene            4824..>5675
                     /locus_tag="VHS06_00860"
     CDS             4824..>5675
                     /locus_tag="VHS06_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX2986715.1"
                     /translation="MSEKLGEHATDITPAARRKLLDSLHYRSVDAGDPTMVASLFTGS
                     TDPVVAYLALPPNLFPPTVRAIDRVGLPAGSRVVLEKPFGDDLDGAVSLNRLLAEFAA
                     HMGDTGERAVYRVDHVLGMATMQNLRGLRQTPVLDAVWNRSQVQEVEILWEETLALEG
                     RAGYYDSAGALKDVLQNHMLQILCLVAMEPPGDPIPVDLPDRRLEVLRSARLLGSRRA
                     RYAAGRLADSGEASGTSVPGYAEEKGVDPGRRTETFVEIGLELTTERWREVPFFLRAG
                     KALSRRRK"
BASE COUNT         1068 a         1741 c         1884 g          982 t
ORIGIN      
        1 accggctcca gtagtacgaa agcttgtcgt tcagagtggg tttggtgatt ggggcagcgg
       61 ccaccagatt cacttcaccg atctccttgt ttcccactac gccaacccgc atggtctgga
      121 tgccagcggg cactactcca ccgctcggga cctcgccact ctcggcgcgt atgccatgca
      181 gaacccggaa ttcgccaaga tcgtgggcac ggccaagatc agcttcagct ggcccggtca
      241 tgacgtgaaa cgggagatgg cgaaccataa caagctgttg ggagtggtcc cgtacgtcaa
      301 cggtatcaag accggctaca cgggcaaagc cggctactgc ctggttgcgt ccggttctca
      361 gaacggcgtc aacctggtca gcgtcatcat gggggaggag accaatcctc aatgcagcgc
      421 ggacacaaaa gcattgctgg actacgggtt ctcgctctac aagaaacaaa cgctggttcc
      481 gagcggcgct gtgctggccg agataccgat cccgtacagg atcggggaaa gcctgaaact
      541 cgttgctgag aaacctctca tccgcaccct ccatctttcc gaggttgtct cgaagaacat
      601 cgagtatcct gagttggcgc ctgtgcctat cgagcagggg caggtgctgg gcaggctcgt
      661 ttgcacggtg ggaaacaagg agatcggtga agtgaatctg gtggccgctg ccccaatcac
      721 caaacccact ctgaacgaca agctttcgta ctactggagc cggttcagcg gctggctgca
      781 gagtcttgtc tcgtagccga tgatcctcac ggttactctc aacccggcgc tcgaccgcac
      841 gctcgccgta cccaacttcg acatcggctt tcgccacagg gcaaccgaga cggtgacgct
      901 gccgggtggg aagggcatca acgtggcccg cgtggtgaag ggcctgggac agccggtgat
      961 agcgaccggt ttcgtgggag gccgaacggg cgagcgcgtc ctttccgacc tggagcgcga
     1021 agacgtgctg tgcgatttcg tgaggatcgg aggggaaacg aggacttcga cggcagtcct
     1081 ggacccaacg gccgggacta ccactgaggt caacgagtac ggtccccaga tacagaagca
     1141 tgaactcgag ttgttgttcg agaaactgga ctatttgagc agggcggcca gcatcgtggt
     1201 gttggccggg agcatcccgc gcaaagtgga caccaacatc tacgcgcagc tgatcggcaa
     1261 ggtgaagcgc ccggacttga ccatcatcgt ggatacatac ggagagccgc tccggcacgc
     1321 cgtcaagagc gggccggatc tcatcttccc caaccagttc gaggcggaga cgctgatagg
     1381 ccacgagttc ggaaccgaag aggagttcat atcggcaacg gccggtctgc ggaagatggg
     1441 cgcctgctcg gcggtattgc acgggcggga tctctgtgtt gcccaagtgg caaagaacgg
     1501 ccaagtcacc accctcgtca cccgtccccc ggcggttgat gtggtgtcta cggtcggttc
     1561 gggcgattca ctggtcggag gctacgccgc ctacatggtt gaaggtcacc cgcccgtcga
     1621 atgccttcgt ttcgggatgg gttgcgccgt ggcaaacacg ctgagctacg gagccggtgt
     1681 cttctcggcg gaggaggcgc gccgcttcgc ggagatcatt gaagtgaagg agtcaatgct
     1741 ggcatgaaag gccgctacga ggagttacac cgggtgctcg tgctggactt cggggcgcag
     1801 tacgcgcagt tgatcgcgcg gcgggtgcgg gaatgcaggg tctactcgga actcattccc
     1861 tacaacacgc cggtcgagga gatccgagcc ctgaaaccgg ccggcctcat cctgtccggc
     1921 ggtcccagga gcgtgaacga gccgggcgcc cccaaagtgg acccagccat cctggatctg
     1981 ggcgttccca tcctcggtat ctgctatgga ctgcagttgc tggtggagat gggcggcggg
     2041 aaggtcggaa aggccgagct gtcggagtac ggaggaacct ccttcttcgt cgtggacgag
     2101 accgagatgt tcaaggggct tccggccgag cagacgtgct ggatgagcca cggggactcg
     2161 gtgctcgagg tgccggccgg ctaccgggtg acggcgcgta gccagcatct cccggtggcc
     2221 gcgtgcgaag acgcggagcg cgagctgtat ggcgttcagt tccacccgga ggtgcggcac
     2281 acgcgcttcg ggctggagat cctcaagaac ttcctctatc gcgtgtgcaa tctggctccc
     2341 acctggaccc ctgtctccat catcgaggat cagatggaga ggatcaggga gcaggtgggc
     2401 aacaacaagg tcatctgtgg gctgtccggc ggcgttgact cggccgtggc cgcccttctg
     2461 gtctaccgcg cggtcggcga tcagctgacc tgcatcttcg tggaccatgg gttgatgcgc
     2521 aaaggtgagg ccgaagaggt cgagaacgcc ttccgcaacc acttccatgt gccgctggtt
     2581 cacgtcaagg ctcaacggcg cttcctcgag aagctgcggg gtgtgacgga gccggaagct
     2641 aagcggaaga tcatcggcaa cgagttcatc cggacgttcg aggaagaggc ggctaagatc
     2701 ggtgactgcc ccttcctggt ccaaggcacg ctctacccgg atgtcatcga gtcgggaaca
     2761 agtaccgccg ccgtgatcaa atcgcatcac aacgtgggcg ggttacctga ggacatggcc
     2821 ttccagcttg tcgagccgct gcggatgctg ttcaaggacg aagtccgagc cgtgggagag
     2881 gaactcggtt tgccggagac aatggtctgg cggcagccct tccccggacc gggactggcc
     2941 atccgtatca tcggcgacgt gacggaggag cgcctccacc tgcttcggga ggcggacgcc
     3001 atccttctgg aggagatccg ccgcgccggg ctctaccggg agatctggca gtgcttcgcg
     3061 gtgctgcccg ccatccgcag cgtcggcgtg atgggcgacg agcgcaccta tgcctacccc
     3121 atcatcatcc gggccgtcac ctctgacgac gcaatgacgg cggactgggc gcgcctcccg
     3181 tatgacgttt tggagcgcat ctccacgcgc atcatcaatg aggtgaaggg gatcaatcgg
     3241 gtggcgctgg acatctccag taagccgcca tccacgatcg aatgggagta atgcccaatg
     3301 ggagcaatgc tcgaacggga gctggacccg aatgggagca agattcgcgg tacagaggac
     3361 gatcagggca acccggtcag ggagccggag gtgcggaagc ggacgaaccg gatccggcga
     3421 ggtggcagat gattgagccg gcggccggag ctccgagtga accgcgcgag cggatccggc
     3481 agccgctgac cgaggagcag cacgaggaga tccgccggcg cgcgcggaaa gcggctgaga
     3541 tagcagcgga tctcaaggcg gaagacatcc gcatcctgga catgcgctcc ctggtcacgt
     3601 acacggatta cctcgtcata tgcacggggc gaagcaccag acagacgagg cgcatctccg
     3661 aggagatagg gtttcgtctg aagaaggagt tcgggctcct gccgctgggc gtcgagggcg
     3721 acgccgccgg cgaatggatc ctcatggact accttgactt catcgttcac gtcttcacgc
     3781 ctgaaacgcg cgagttctac cggctcgacg tgatgtggaa gcaggccccc accgaagtgt
     3841 tggcgcccgg gagcgaaacg ggcgcggagg ctacggaggg ataggcccgt cagcggagcg
     3901 cggcaccgca ccgcgctctc agctttgccc gggctcgctt gccaaggacg tctcgccgcc
     3961 ggcgaggccg gagtagcggc ggacccgagg cgcggctgct ggaatgggcg ctgggccccg
     4021 ggtttgcttg cagccggcgt gccccctggg tataatccat ccccggcctc gtgggggtat
     4081 agctcagctg ggagagcgcg acgctggcag cgttgaggcc aggggttcga gtccccttac
     4141 ctccacctca cagaagcggt aatcgaagac ccggtctcaa cagccgggtc ttcggcgttt
     4201 ccggggctgt ctcaacagcg cctcacccga ctcgccgcac cccggacccc agcccttgtt
     4261 gcaccctttt ggacgccatc cgacccgtct tggcccttca ttgcaagctt tcacgcggcg
     4321 gcccgtcgga gcctgccggg tactcttgaa gtgggacgcg gtcctcatgc cacgcctcga
     4381 ggaccggtgt caccaccctc catgcctcct cggcctcgtc tgcgcggacc gaaaggacat
     4441 tgccgccgct gaggacatcg aggagcaccc gggcataggc gggcaggagc ggccgcggag
     4501 gttcgccggc gagatcgatg ggcacgggcc gcccagccgc acggccggcg aggcgcaaca
     4561 cgatgtcgcg gggaccgtcg atcccgatcc agagttcgtt cgcgacttgg ccgccgcccg
     4621 ggcggaaatg gatgacgact cctttgcgtc tccggctcaa ggctttgcct gcacgcagga
     4681 agaacggcac ttcgcgccag cgctcggtcg taagctcgag gccgatttcg acgaaggtct
     4741 cggtgcggcg tcccgggtcc actcccttct ccatcatggg cgcctctcgt caagggctgg
     4801 atgaaagtgc gtttcgggac agcgtgagcg aaaagctcgg cgaacatgcc accgacatca
     4861 cgcccgcagc acgccggaag ctgctggact cgcttcacta tcggagcgtc gacgccggcg
     4921 accccaccat ggttgcctcg ctgttcaccg gctccaccga ccccgtggtg gcgtatctcg
     4981 cgctaccacc gaacctcttc ccccctaccg tccgcgcgat cgatagggtc ggcctgccgg
     5041 ccggtagccg tgtcgtgctg gagaagccct tcggggacga tctggacggc gccgtatcgc
     5101 tcaatcggct tctggcagag ttcgctgccc acatgggcga cacgggcgag cgagccgtgt
     5161 atcgagtgga ccatgttctc ggcatggcaa ccatgcagaa cctgcggggg ctgcggcaga
     5221 ctcctgtcct cgacgccgtc tggaacagga gtcaggtgca ggaggtggag atcctctggg
     5281 aagagacgct ggcgcttgaa ggccgagccg gctattacga cagcgccggg gcactcaaag
     5341 atgtgctaca gaaccacatg cttcagatcc tgtgtctggt ggcgatggag ccccccggag
     5401 acccgatccc cgtcgacctc cccgaccgca gactggaggt cctgcgttct gcgaggctac
     5461 tcgggtcccg gcgcgcccgc tacgcggcgg gcaggctggc cgactccggg gaagcgagtg
     5521 gaacctccgt accggggtac gccgaagaga agggagtgga cccgggacgc cgcaccgaga
     5581 ccttcgtcga aatcggcctc gagcttacga ccgagcgctg gcgcgaagtg ccgttcttcc
     5641 tgcgtgcagg caaagccttg agccggagac gcaaa
//
LOCUS       DATALS010000044         3822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_407327, whole genome shotgun sequence.
ACCESSION   DATALS010000044 DATALS010000000
VERSION     DATALS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407327"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VHS06_00865"
     CDS             <1..298
                     /locus_tag="VHS06_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986716.1"
                     /translation="IEKIAAVSEETSASAEEVSASAEEMSAQVEEMVAQAQELAQMAE
                     ELQAAVAQFKLGDGAARGETVARRRKDDWTGHQPRREAERQPQLQSVSRSQARR"
     gene            303..773
                     /locus_tag="VHS06_00870"
     CDS             303..773
                     /locus_tag="VHS06_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX2986717.1"
                     /translation="MSDDKAYGEEQLVVFELGEEAYGVDIGRVQEIIRMTAITKVPQA
                     PEHVEGVINLRGKVIPVIDLKRRFGLPEGSGAKAGRVVVIDVAGHTVGMVVDGVSEVL
                     RLHMADVEPPSPVVTSIEATYLRGIAKLDGRLILLLDMDKLLTWEERQQLNEAA"
     gene            complement(757..1008)
                     /locus_tag="VHS06_00875"
     CDS             complement(757..1008)
                     /locus_tag="VHS06_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986718.1"
                     /translation="MAQITEDALDIDSNVGVGIPAWSAGNERPPITLTPLARLVVVIG
                     RVAKRERWLELGNGKGIGRRSYLPIPLLVVAWTCLRPPR"
     gene            complement(1067..1585)
                     /locus_tag="VHS06_00880"
     CDS             complement(1067..1585)
                     /locus_tag="VHS06_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986719.1"
                     /translation="MNHDDLRLRIGRALSDLGVSGHRDTTDPLRRPRWRASFSSHGVV
                     EGETRYLQLFVPVDDDILQLNCTVEASVLDAMPQEQLQGSLEEFGVQCGHGTRQWESL
                     LADLGVDPGDTDGWSATIALHTGIYHRMVLTRVTMPLEHLSDRSIELATRIGYRLSGD
                     ARARMATRCEAS"
     gene            complement(1626..1919)
                     /locus_tag="VHS06_00885"
     CDS             complement(1626..1919)
                     /locus_tag="VHS06_00885"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HEX2986720.1"
                     /translation="MKNPEPRTQNSAPTSRPSLRAVRQLQKELATVEREIISLEQRVA
                     ELEKVMADSPELLVDQEKSAPLVAEYTDLTTRLPELNQRWEEIGLKLEDAQPA"
     gene            complement(1948..3633)
                     /locus_tag="VHS06_00890"
     CDS             complement(1948..3633)
                     /locus_tag="VHS06_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX2986721.1"
                     /translation="MLQASKITKHFGGDTILDGIDLELNPGERVALVGPNGAGKSTLL
                     RILLGELEPDAGKVHLLSGATVAYLPQDAGVVPGRTLHDEMLALFADVIELDDRQREL
                     EAEMASLPPGSPRLMDLVNTQAELHSEFERRGGYTLEAEIGRVLYGLGFSMSDYDKRV
                     ENFSGGWQMRIALARLLLQRPDLLLLDEPTNHLDLRATEWLEGFLRQYRGAVMVVSHD
                     RYFLDLVATRTLELHRCHLIVYPGNYSFYVKEKARRQKEQEAAFKRQQDYLQRQQAFI
                     ARFGADKRRSSQTQSREKLLEKLERIEAPSGVQKAIKFRFPPSTPSGRKVFELRGTGK
                     AYDDRWVFQGSDLLVEKGDRVALVGPNGAGKSTLLRLLAAVEKPDEGTVSVGANVLRA
                     YYAQDQSESLDVTNTVLQELYTAAPKDWTLEDVRSMLGRFLFSGDDVYKSISVLSGGE
                     RSRLALAKMLLRASNVLLMDEPTNHLDIAARETLEDALTEYHGTLILASHDRYLIDKL
                     ANKVVEIDGGVVTLYPGNYTAYRTAKAQEAETAGRGDAETRRNGESPLPPGRG"
BASE COUNT          674 a         1155 c         1246 g          747 t
ORIGIN      
        1 catcgagaag atcgcggcgg ttagcgagga aacgagtgcc tccgctgagg aggtgagtgc
       61 ctcggctgag gaaatgagtg cccaggtgga ggagatggtg gcgcaggccc aggagttggc
      121 acagatggcc gaggagctac aggcagcagt tgcccagttc aagttggggg acggggccgc
      181 caggggcgag acggtcgcgc gccgccgcaa ggacgactgg acagggcacc aaccccggcg
      241 agaagcggag cggcagcccc aacttcaatc ggtctcgcgg tcgcaggcaa ggaggtaagg
      301 cgatgtcgga tgacaaggct tatggcgaag aacagttggt cgtcttcgag ctgggcgagg
      361 aagcctatgg ggtggacatc ggtagggtgc aggagatcat caggatgacc gccatcacga
      421 aagtccccca ggcacctgag cacgtagagg gcgtgataaa cctccggggt aaggtgatcc
      481 ctgtgatcga ccttaagcga cgctttggtt tgcccgaggg aagcggggcg aaggccggcc
      541 gggtcgtggt gatcgatgta gccggccaca ccgtcggcat ggttgtggac ggtgtgtcgg
      601 aagtactcag gttacacatg gccgacgtgg agccgccctc accggtggtt acatctatcg
      661 aggcgactta tttgcgaggg atcgccaagc tggacgggcg gctgatcctc ttgctggaca
      721 tggacaaact gcttacctgg gaagaaaggc agcaactcaa cgaggcggcc tgagacaggt
      781 ccaggcgact acaagcaagg ggattggaag gtagctgcgc cttccaatcc ccttgccgtt
      841 gcccagttcg agccatcgct ccctcttggc gacacgacct atgacgacaa cgagccgcgc
      901 caggggtgtg agggtgatgg gcgggcgctc gttaccggca gaccaggcgg gtatccccac
      961 accgacatta gagtcgatgt ccagcgcatc ttccgtgatc tgcgccattg atccaagaag
     1021 agcttcaacg gccagtgcaa ggccatcttc cactcgtagg ccgttttcag cttgcctcgc
     1081 agcgcgtggc catccttgct cgagcgtccc ccgatagacg gtatccgatc cgtgtggcaa
     1141 gctcgatcga gcggtcggag agatgctcaa gcggcatggt cacgcgggtc agcaccatcc
     1201 ggtggtagat ccctgtgtga agggctatcg tcgcactcca cccatcggtg tcgccggggt
     1261 caacgcccag gtcggcgagc agagactccc actgccgcgt cccgtggccg cactggacgc
     1321 cgaactcttc gagggagccc tgaagctgtt cctgcggcat cgcgtcgagc acagacgcct
     1381 ccacggtgca gttcagttgg aggatatcgt cgtcgacggg gacgaacagc tgaagatagc
     1441 gggtttcccc ctctaccaca ccgtgagacg agaaggacgc tctccatctg ggccttcgta
     1501 gagggtcggt ggtgtccctg tggccggaga cgcccaagtc tgacagcgcc cggcctattc
     1561 tgagacgaag atcatcgtgg ttcactgatc gttgttctcc gtgtaaagcg ggcaaacgcc
     1621 cgtcactatg cgggctgagc gtcttccaac ttcagcccaa tctcctccca acgctggttc
     1681 agctcgggga ggcgtgtcgt gaggtcggtg tactcggcga ccagcggtgc cgatttctcc
     1741 tggtccacca gtagttccgg cgagtcagcc atcaccttct ccagttcggc cacacgctgc
     1801 tccagggaaa tgatctcccg ctccaccgtt gccagctctt tctgcagttg gcgcactgcg
     1861 cggagcgacg gtcgggaggt gggtgctgag ttctgggttc tgggttctgg gttcttcacg
     1921 gaagtgctgg gtgctgagcg ctgggtgcta ccctctccca ggggggagag gggattcccc
     1981 gtttctccgc gtctccgcgt ctccgcgtcc cgccgtctcg gcctcctggg ctttggctgt
     2041 tcggtaggcg gtgtagttgc ccgggtagag ggtcaccacg ccgccgtcga tctccaccac
     2101 cttgttcgcc agcttgtcga ttagatagcg gtcgtgggag gcgaggatca gcgtcccgtg
     2161 gtactccgtg agggcatcct ccagtgtctc cctggcggcg atgtccaggt ggttggtggg
     2221 ctcgtccatg agcaggacgt tggacgccct cagcagcatt ttcgcgaggg cgagcctgct
     2281 tcgctcgccg ccgctcagca cggaaatgga cttgtacacg tcgtcgccgc tgaacaggaa
     2341 ccggcccagc atggagcgca catcctccag cgtccagtct ttgggcgcag cggtgtacag
     2401 ttcctgcagc acggtgttgg tcacgtccag gctttcggac tggtcctgcg cgtagtaggc
     2461 cctcaacacg ttggctccca ccgagacggt gccctcatcc ggcttctcca cggcggccag
     2521 tagccgcagg agggtggact tgccggcgcc gttgggccca accagcgcca ctcgatcgcc
     2581 cttttccacc agaaggtcgc ttccctgaaa cacccatcgg tcgtcgtatg ctttaccggt
     2641 gccgcgcagc tcgaagacct tccggccgct gggcgtggaa ggtggaaagc ggaacttgat
     2701 cgccttctgt accccggagg gagcctctat ccgctccagt ttctccagta gcttctcccg
     2761 actttgggtc tggctggaac gccgcttatc ggcgccgaag cgggcgatga aagcctgctg
     2821 ccgctgaagg tagtcctgct gccgcttgaa ggcggcctcc tgttccttct gccgtcgggc
     2881 cttctccttg acgtagaagg agtagttgcc cgggtagacg atcagatggc agcggtgcag
     2941 ttccagcgtg cgcgtggcca cgaggtccag gaagtagcgg tcgtgggaga ccaccatcac
     3001 ggccccgcgg tactggcgta agaaaccttc cagccactcc gtggcgcgca ggtccaggtg
     3061 gtttgtgggc tcgtccagaa ggagaaggtc ggggcgctgc agcaacagcc gggcgagggc
     3121 gatgcgcatc tgccacccgc cgctgaagtt ctccacgcgc ttgtcataat ctgacatgga
     3181 gaagccgagg ccgtagagga cccttccgat ctccgcttcc agggtgtacc ccccgcgccg
     3241 ctcgaactcg gaatgcagct cggcctgggt gttcaccaga tccatcagcc tgggcgaccc
     3301 cggcgggagg ctggccatct cggcctccaa ctcccgctgc ctgtcgtcga gctcgatcac
     3361 gtcggcgaag agcgccagca tctcatcgtg aagggtgcgg cccgggacta caccggcatc
     3421 ttgcgggaga taggcaacag tagcccccga taggagatgc accttgcctg cgtcgggctc
     3481 cagttctccc aggaggatgc gaagaagggt cgacttgccg gcgccgttgg ggcctaccag
     3541 agcgactcgc tcgccggggt tcagctcgag atcgatccca tccaggatcg tgtctccgcc
     3601 gaagtgtttg gtgatcttac tcgcttgcaa catacagtca tattgtacca gtaagctacc
     3661 gggttttgcc cgtctcgaag tagtcgtagg ctgtcttcgc cacctgggcg atggactgag
     3721 ccgccaggtt gtagtcggcc agatccgtgt tgagcaccgt cactacgaac tggctcctgg
     3781 gcgcgtaact gatcccggct tcgttgcgaa cgccaggcag tt
//
LOCUS       DATALS010000045         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_183909, whole genome shotgun sequence.
ACCESSION   DATALS010000045 DATALS010000000
VERSION     DATALS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183909"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VHS06_00895"
     CDS             complement(<1..765)
                     /locus_tag="VHS06_00895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2986722.1"
                     /translation="MKGTPPPMATPPEVTSGQPKASLPESDSRPHGFVPHTDATRPHV
                     DENQLLTLLVVGMFLVVTTINMMAPLLVDFAADFHTTVGAMGLLAAACGVPWAILAPF
                     MGALSDRYGRRPVFMTGVFVLGLSTVASAFAWDYSSLLVIRIIGGIGGASTGPNIMSS
                     AADYFPASRRGRALGLVVAAVSMATVIGVPAVAEMAALFGWRWAFAGQGVLLLLLGEG
                     IWRLFPRWKAYTRTADSLSGLSQVLAERSTRMLLVSN"
     gene            986..>1673
                     /locus_tag="VHS06_00900"
     CDS             986..>1673
                     /locus_tag="VHS06_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX2986723.1"
                     /translation="MNVEVVFLPPIATELPSKLCVVIDVLRATSTLVSIFEAGAERVV
                     LAESIPAALAYADGSKERPLVCGESGGLAPDGFDLGNSPREFRPEAVKGKELCFCTSN
                     GTRAMRRVSSSPVVFAGCLLNGTSLARRAVREASARELDIAVVCSGDILGSKFAIDDA
                     FTAGYLVKLMQREAAGPTVADAEADRRFVHARAIREDEAVDLELEESATAAIRLYESY
                     LLEAGASDLDA"
BASE COUNT          298 a          488 c          586 g          301 t
ORIGIN      
        1 gttggacacc agtagcattc gagtggatct ctccgccagc acctgcgaaa gaccggatag
       61 agagtcggcg gtcctggtgt atgccttcca tcgggggaag agccgccaga ttccctcacc
      121 cagcagtaga aggagcacac cctgaccggc gaaggcccaa cgccatccaa agagcgcggc
      181 catctctgct accgcgggca cgccgattac cgtggccatg gagactgcgg ccaccaccaa
      241 tcccagggca cgcccacggc ggctggcagg gaagtagtcg gcggcactgg acatgatgtt
      301 cggaccggtg gaggcgccgc cgatcccgcc gatgatacga atcaccagca gcgacgagta
      361 gtcccaggcg aaggcagagg ccactgtgct gagcccgagt acgaagacgc cggtcatgaa
      421 gactgggcgg cgcccgtagc ggtcggacag cgcacccatg aagggagcga ggatagccca
      481 cggcactccg catgctgcgg ctaacaaccc catcgccccg acggtagtgt ggaaatcggc
      541 tgcgaagtcc acgagcagtg gggccatcat gttgatggtc gttaccacca ggaacatacc
      601 gacgaccagg agtgtcagca gttggttctc gtcgacgtgc ggtcgggtgg cgtcggtgtg
      661 gggcacgaaa ccgtgcggcc ttgagtcgct ttctgggaga cttgccttgg gctggcctga
      721 ggttacctcg ggtggggttg ccatgggagg cggtgtgcct ttcatgggga cctggctcgc
      781 tcgacacttt cagcgcggcg aggcccggca catccagttc cggcggtatg caggggacag
      841 ccgtgcccca ggtctctctc aattgatggt gcctgacgat tgcggcagta tagcacggct
      901 gaggtggcgg cgcccgccgt ggtagaatcg atggttggcg gtcctgtttg atgtgatgat
      961 agccgtctaa ggcggaggtg gttggatgaa cgtcgaagtt gtctttcttc cccccattgc
     1021 cacggagttg ccctccaagt tgtgtgtagt catcgacgtg ctgagggcta cgtccaccct
     1081 ggtttccata ttcgaagccg gggccgagcg ggtcgttctg gccgagagca tccctgccgc
     1141 gctggcttac gccgacggca gcaaggagcg gccgctggtg tgtggagagt cgggcggact
     1201 ggcaccggat ggcttcgatc tcgggaactc cccgagggag tttcggccgg aggcggtgaa
     1261 ggggaaggaa ctgtgcttct gcacatccaa cggaaccagg gcgatgcggc gggtatcgtc
     1321 ctctccggtg gtgttcgccg ggtgcctgct caacggcacc tcgctggcca ggagggcagt
     1381 gcgtgaggcg agcgcgcgtg aactcgatat cgccgtcgtc tgttcgggcg acatcctggg
     1441 cagcaagttt gccatagacg acgccttcac ggcgggctac ctcgtaaagc tgatgcagcg
     1501 tgaggctgct ggaccgaccg tcgccgatgc cgaggccgat agacgcttcg tgcacgcgcg
     1561 ggccattcgg gaggatgagg cggtcgacct ggagcttgag gagtcggcca cggcggccat
     1621 ccgtctttac gagagctacc tgctggaggc cggggcctcg gacctggacg ccc
//
LOCUS       DATALS010000046         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_92240,
            whole genome shotgun sequence.
ACCESSION   DATALS010000046 DATALS010000000
VERSION     DATALS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92240"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VHS06_00905"
     CDS             <1..334
                     /locus_tag="VHS06_00905"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX2986724.1"
                     /translation="ILNIILLIMGCFLESLSTMMLLVPVLMPLIKTVGIDPVHFGVVL
                     TLNLMIGLITPPVGMSMFIGCAIAKIKISDFAKEIPIMIAALILVLLMITYIPELVLF
                     LPNLIMGR"
     gene            357..1076
                     /locus_tag="VHS06_00910"
     CDS             357..1076
                     /locus_tag="VHS06_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2986725.1"
                     /translation="MLIDEQACSGCGECVPYCTVAAISLGDDGVSHIDLDVCVECNAC
                     LRAEVCPSDAIYQQPMDELRSLRGQFSDPLGVHPKTGVPGRGTEEIKTNDVTDRVKPG
                     FAGIAVEVGRPSVGTRLAEVEKIAMALSKIGVQHEPANPVTFLMTDKQTGKMRDEVLG
                     ENVLSAIIEFIVPESKVVDVFSVLKEVEPRVDTVFSVDLAVRYGADGTVPIKKVVEDA
                     GFKLRPNGKTNIGMVTHLKGR"
     gene            1089..1718
                     /locus_tag="VHS06_00915"
     CDS             1089..1718
                     /locus_tag="VHS06_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986726.1"
                     /translation="MTHTLHRRGRVEDLAKDYVFTTMASKGINVPGTAEEMKKFYEVV
                     LKHNPTFVGDAAKGNQLTLGIRPMVEGTEDRTIGHAVFRDADTVAAVIADLIEVGFNR
                     SVVVSGLFENVKECCQKAGIGTPHTIEYSLETWGKTEKMAPMDELEVMTMCGHAQVPG
                     ALVRHVAQQVKAGKLTAEEAGKRLGRQCACGVFNVARASELVAAMAARM"
     gene            complement(1967..>2461)
                     /locus_tag="VHS06_00920"
     CDS             complement(1967..>2461)
                     /locus_tag="VHS06_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2986727.1"
                     /translation="AGMPMVAPSEELELSAWQRTLDTNLTGCFLCAQAAGRVMLRQGS
                     GSIINMASLTSYLGFPGRLAYSTTKSALLGLTRTLSSEWAPKGIRVNAIAPGWILTDL
                     LQSVIDKGALDPQKLITRTPIGRIGTPEDVVGPAIFLASDESAFVTGQVLPVDGGWLS
                     YAFM"
BASE COUNT          516 a          696 c          810 g          439 t
ORIGIN      
        1 gatcctcaac atcatcctgc tgatcatggg ctgtttcctg gagagtctat caaccatgat
       61 gctgctggtg ccggtgctga tgccgctgat caagaccgta ggcatcgatc cggtccactt
      121 cggcgtagtg ctcaccctga acctgatgat cggcctcatc acaccccccg tcgggatgtc
      181 gatgttcatc ggctgcgcca tcgccaagat caagatctcc gacttcgcca aggagatccc
      241 gatcatgatc gccgcgctca tcctcgtact gctgatgatc acctacatcc cagagctcgt
      301 gctgttcctg cccaatctca tcatgggcag gtgacgaatc tacaaggaga agtggaatgc
      361 tgatcgacga acaggcgtgc agtggatgcg gagagtgtgt gccctattgc accgtggccg
      421 ccatctcgct gggtgatgat ggggtttccc acatcgacct cgatgtgtgc gtcgagtgca
      481 atgcctgcct tcgggcagag gtgtgccctt cagatgccat ctatcagcag ccgatggacg
      541 agctgcgctc cctgcgaggt cagttcagcg acccgttggg cgtacatccc aagacgggcg
      601 tgcccgggcg gggaaccgag gagatcaaga ccaacgacgt gaccgaccgc gtcaagccgg
      661 gctttgccgg catagcggtg gaggtgggcc ggcccagcgt tgggactcga ctggccgagg
      721 tcgagaagat cgccatggcg ctctccaaga taggtgtgca gcacgagcct gccaacccgg
      781 tgaccttcct gatgacggac aaacagacgg gcaagatgcg agatgaggta ctgggtgaga
      841 acgtgctctc ggcgatcatc gagttcatcg tgcccgagtc caaggtggta gatgtgttca
      901 gcgtcctgaa ggaggtagag ccgagagtcg acacagtctt cagcgtggac ctggctgtga
      961 ggtatggtgc tgacgggact gtgccgatca agaaggtggt ggaggacgca ggcttcaagc
     1021 tcaggccgaa cgggaagacc aacataggca tggtcaccca tctgaagggt cggtgaggag
     1081 agcataagat gacgcacaca cttcatcgta ggggcagggt agaggatcta gccaaggatt
     1141 acgtcttcac gaccatggca tccaagggga tcaacgtccc cgggacagcc gaggagatga
     1201 agaagttcta cgaggtggtg ctgaagcaca acccgacctt cgttggcgac gcggccaaag
     1261 gcaaccagct gaccttaggg atccgaccga tggtggaggg gactgaggat cggaccatcg
     1321 ggcacgcggt gttccgggat gcggacacgg tggcggccgt catagcggat ctgatcgagg
     1381 tggggttcaa ccggtccgtg gtagtcagcg gcctcttcga gaacgtgaag gagtgctgcc
     1441 agaaggccgg aatcggcact ccacacacca tcgagtactc gttggagacc tggggtaaga
     1501 cagagaagat ggctcccatg gacgagctgg aagtaatgac gatgtgcggg cacgcgcagg
     1561 tacccggggc actggtccga cacgtggcac agcaggtgaa agcgggcaag ctgacggccg
     1621 aggaggccgg caagagactg ggcaggcagt gcgcctgcgg cgtattcaac gtagccaggg
     1681 cttccgagct ggtagccgcc atggccgcga gaatgtgaga agaaagcagg tccaggggcg
     1741 cagcccctgg cgagggttgg ggtgtcccca aaatcacact tactactagc tgctaggatc
     1801 gacggggcgg gtcgagactc gacccgcccc tcttgattct ggcaggatcg atccgtaggg
     1861 cggggtcttg tgccccgcca aatgcatggg cggattatcg cccggtggtc gtagccacgg
     1921 tgtggtaggc ggggcacaag accccgccct acaacacgat gctaggctac atgaaggcgt
     1981 aggagagcca gccaccatcc accggcagca cctgcccagt aacgaaggcc gactcgtctg
     2041 aggccaggaa gatggcaggc cccacgacat cctccggcgt tccgatccga ccgatggggg
     2101 tccgtgtgat cagcttctgc gggtccagcg cccccttgtc gatgacgctc tgcagcaggt
     2161 ccgtcaggat ccatcctggg gcaatcgcgt tgacccgtat ccccttgggt gcccactcgg
     2221 aggagagggt tcgagtgagt cccagcagag cggacttcgt cgtgctgtag gccagccggc
     2281 cggggaagcc gagataggag gtgagagaag ccatattgat tatgctgccg gagccctggc
     2341 gaagcatcac tcgtcccgcc gcctgggcac acaggaaaca gcccgtcaga ttcgtgtcca
     2401 gcgttcgctg ccaggccgac agctccagtt cctcactggg ggcaaccatc ggcatccctg
     2461 c
//
LOCUS       DATALS010000047         6029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_364091, whole genome shotgun sequence.
ACCESSION   DATALS010000047 DATALS010000000
VERSION     DATALS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364091"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VHS06_00925"
     CDS             <1..603
                     /locus_tag="VHS06_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986728.1"
                     /translation="LYPCAREAEDLYHFEDDCGHDPHMWLCERHHWQYTQPGGPKREL
                     IQLDWQSLGYPEMLDSADGKEPLEDPNGIDGVVDWERMMPDGAISRFVLSHDTGRMWE
                     IRYDPSGRAIEARMVAEGSDVWTAVDWQAFGIDAVEAEEAPDELHGYVSFERLDGDGG
                     VVRHIYSRRSNRLWQLHLNRDGAVDSVLPAPDDLMAEDIC"
     gene            complement(600..887)
                     /locus_tag="VHS06_00930"
     CDS             complement(600..887)
                     /locus_tag="VHS06_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986729.1"
                     /translation="MVKQRCEALDHRARQCGKPGARLVRNLGWLCKDHDPDTLAPPLG
                     QLCAAEGCGAPAENYDDSFGLWYCPQHTVEQWRGPCTALVQTLYPCAAAKP"
     gene            1238..2881
                     /gene="treS"
                     /locus_tag="VHS06_00935"
     CDS             1238..2881
                     /gene="treS"
                     /locus_tag="VHS06_00935"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX2986730.1"
                     /translation="MQSDRLWYKDAVFYEVPVHAFHDGNGDGVGDFRGLKKKLGYLRD
                     LGVDCLWLLPFYPSPMRDGGYDVSDFFQIHPDYGTLEDFRDFIKAAHQHGIRVIGDLV
                     LNHTSDQHPWFQESRRSPDSPKRDWYVWSDDPSKYKEARIIFCDLEPSNWTWDPDAKA
                     YYWHRFFSHQPDLNYDNPEVREAMKDVVRYWLGMGLDGFRCDAVSYLFEREGTSCESL
                     PETHAYLRELREMVDREFPGCIMLAEANQQPSALCEYFGDCNEFHMAFHFPLMPRVFL
                     AMARESCEPIVNILGETPEIPDGAQWGIFLRNHDELTLEMVSEDEREHLYREYVLDPR
                     MRLNEGIRRRLAPLLSNDRRQLELVHAMLFSMQGSPILYYGDEIGMGDNIYLGDRNGV
                     RTPMQWSARQNAGFSSADPESYYLPLVDDPCYCCQEVNVEAQQSNPHSLLSWMRRMIG
                     IRRQLPAMGRGSMEILPTSNSKLLAYLRQYRDQTVLAVNNLSNHPQQCELDLRRFQGY
                     LPVDVLQKGSLLTIGNAPTSLALGPLQYCWFVLEPQSAA"
     gene            2994..5978
                     /gene="treY"
                     /locus_tag="VHS06_00940"
     CDS             2994..5978
                     /gene="treY"
                     /locus_tag="VHS06_00940"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX2986731.1"
                     /translation="MSRLLAGRRPTSSYRWQFNSAFTFRDAARLVPYLHDLGVTDCYA
                     SPYLQARPGSPHCYDVCDPGTINPELGAAEEFEAFVSALKERDMGLLLDVVPNHMSSW
                     ATGNRLWRDVLENGPSSPYARFFDVDWHPLRSDTLLEDRVLLPILGDSYGTVLERQEL
                     QLFFEDGAFQLRYYENLLPLDPSSYIEILRLCEERLSDSMDVGEDNLRELQSIITAIS
                     HLPSRTERDPERVQERQREKEVVKRRLNALYEASAQVGAAVNEAVGLFNGISGDPSSF
                     DRLDALLEQQPYRLAHWQVAAEEINYRRFFDVSDLVALRQEDPEVFDETHRLILKLLG
                     EGTVTGLRIDHVDGLWDPAAYLSRLQRSYLEELGSVRATEEDSGRRLYLVVEKILRPG
                     EKLPPDWPVDGTTGYDFANAVNGIFVDNSNARSFDVLYRRFIGFDMDFQEQLIAAKKR
                     VMLTSLASEVNMLAYQLRRIAARNRNYRDLTLNGLTFGLRETIACLPVYRTYISRGDR
                     ELRERDRTYLEGALAEARRRNPSAPAAVFDFVAEILLLRWPAGVKDEDRGQLWDFVRD
                     FQQVTGPTMAKAMEDTVFYVYNRLISLNEVGGDPGRFGISVDDFHQQNALRLQEWPYS
                     LTSTTTHDTKRSEGVRARINLLSEMPREWKAMLGRWSKSNGRRKRTVSGRPVPDRNEE
                     YYLYQTLLGSWPVGGMDGEQYRSYQERVEAHMIKAIREAKVNTSWVNPNSQYEDAVRH
                     FVGSILNQQRANPFLKSFQPFQLSIARYGMVNSLSQTLLKIASPGVPDLYQGTELWDL
                     YLVDPDNRRPVEYGPRLEMLERIQEGRETGERLSDLAAELLAHWEDGGVKLHLIHRAL
                     SYRRRHPDLFTRGDYVPLAATGRHRDRVCAFARRDEDATVVVVVPRLVWGLMQGKADK
                     PGVDGGGQWLPLGEGIWEDTRLLLPSDWAAGGFRNLLTGEELETVETRGRERSCPDCG
                     HTGRQAMVSRSPKVRPLRVRSR"
BASE COUNT         1153 a         1798 c         1902 g         1176 t
ORIGIN      
        1 ctctacccct gtgccaggga agccgaagac ctctaccact tcgaggatga ttgtggccac
       61 gacccgcaca tgtggctatg cgagcgccac cactggcagt acacgcaacc gggcggcccc
      121 aaacgggagt tgatccagct agattggcaa tcgctcggat acccggagat gctggattcc
      181 gcggacggaa aggagccgtt ggaggatccc aacggcatag acggcgtcgt tgactgggag
      241 cgaatgatgc ccgacggcgc tatcagccgt ttcgtcctca gccacgacac agggcgaatg
      301 tgggaaatcc ggtacgatcc atccggtcgc gcaatcgaag caaggatggt ggccgagggc
      361 tcggacgtat ggaccgcggt ggattggcag gcgttcggga tcgacgcggt cgaggccgag
      421 gaggcgccgg acgagctgca cgggtacgtg agcttcgagc ggctcgacgg ggacggaggt
      481 gtcgtccggc acatatacag tcgacgatcg aacaggctct ggcaactcca cctgaacagg
      541 gacggagctg tggactcggt gcttccggcg ccggacgacc tgatggctga agacatctgc
      601 tagggcttgg ccgcggcaca ggggtagagt gtctgaacca gtgctgtaca gggacctctc
      661 cattgctcca ccgtgtgctg tgggcagtac cacaggccga agctgtcgtc gtagttctct
      721 gccggggcac cgcagccttc ggccgcgcag agttggccca ggggaggggc cagcgtgtct
      781 gggtcgtggt ctttgcacag ccagcccagg tttcggacta gccttgctcc cggttttccg
      841 cactgtctgg ccctatggtc taacgcttcg cagcgctgct tcaccattga actgttctct
      901 ccgtctttct agcacttagg caagttcgat ggacagtccg gcgggatccg cgatctccac
      961 tcctgggcgg tgctcttgaa ccggcaaccg gcgcccacta ccttccatcc cacattatac
     1021 cctgcttaac ggacgtgtcc ggtgggccaa gcaggaggcc cccttggctt tttactattg
     1081 attgactatt tagcctgtat atgtcacaat ttgtgtacat ttgcggccga aacgttgggt
     1141 ctggtgcatt gcccaggccc accatttggc tcatttccgc ctccagatct tcagcctgac
     1201 cttggggacg ccaagagggg caacgatgcg caacatgatc cagtctgacc ggctctggta
     1261 taaggatgcg gtgttttacg aagtgcccgt gcacgccttt catgacggca acggggacgg
     1321 cgtgggggat ttccgaggcc tgaaaaagaa gttaggctac ctgcgcgacc tgggggtgga
     1381 ctgcctctgg ctgctgccct tctatccgtc tcccatgcgc gacggtggct acgacgtctc
     1441 agatttcttc cagatccatc ccgactacgg caccctggag gatttccgcg acttcatcaa
     1501 ggcggctcac cagcacggga tcagggtgat cggggacctg gtgctgaacc acacctcgga
     1561 tcagcacccc tggttccagg agtcacgccg ttctcccgat tcccccaagc gtgactggta
     1621 cgtgtggagc gacgacccct ccaagtacaa ggaggcacgg atcatctttt gtgacctcga
     1681 gccctcgaac tggacctggg accccgacgc aaaggcctac tactggcatc gtttcttcag
     1741 ccaccagccg gatctgaatt acgacaatcc cgaagtgcgc gaggcgatga aggacgttgt
     1801 ccgctactgg ctggggatgg ggctggacgg cttccgctgc gacgcggtgt cctatctctt
     1861 cgagagggaa ggtaccagct gtgagagttt gccggagacc cacgcctacc tgcgtgagct
     1921 tcgggagatg gtcgacaggg agttcccggg ctgcatcatg ctcgccgagg cgaaccagca
     1981 gccttcggct ctgtgcgagt acttcgggga ctgcaacgag tttcacatgg cgttccactt
     2041 tccgctcatg cccagggtct tcctcgccat ggcgcgggag agctgtgagc cgatagtgaa
     2101 catcctgggg gagacgccag agatccctga tggggcccag tggggcatct ttctgcgaaa
     2161 ccacgatgag ctcacgctgg agatggtgag cgaggacgag cgggagcatc tctatcgaga
     2221 gtacgtcctg gatccaagga tgcggttgaa cgagggcatt cgacgtcggc tggctccgct
     2281 gctcagcaac gatcgccgcc agcttgaact ggtgcacgca atgctcttct ccatgcaggg
     2341 cagcccgatc ctctattatg gggacgagat cggcatggga gacaatatct accttgggga
     2401 taggaacggg gtgcgcactc ccatgcagtg gagcgcccgg caaaacgccg gcttctcatc
     2461 cgcagaccca gagtcttact acctgcccct ggtggacgat ccctgctact gctgccagga
     2521 ggtgaacgtg gaggctcagc agtctaaccc ccactcgctt ctttcgtgga tgcgacggat
     2581 gatcgggatc aggcggcagc ttcccgcgat ggggcgaggt tccatggaga tccttcccac
     2641 cagcaactcg aagttgctgg cctacctgcg ccagtatcga gatcagaccg tcttggcggt
     2701 caacaatctg tccaaccacc ctcagcagtg cgagttggac ttgcggcgct tccagggcta
     2761 cctgccggta gacgtacttc agaagggcag cctgctaacc atcggcaatg cccccaccag
     2821 cctggcgctt ggcccgctcc agtactgctg gtttgtatta gaacctcaat ccgccgcatg
     2881 atgaaaggca agaagcctgc cgcacgggca ggcttcttgc ttgtcaccgc cccagggggc
     2941 ggaccatgca agacgattcc ggcgctgtga gacgactgcc tggcgagcat gacatgtctc
     3001 gtctgcttgc cggccggagg cccacttcca gctatcggtg gcagttcaac agtgcgttca
     3061 cctttcggga tgccgcgcgt ctggtcccct accttcacga tctcggcgta acagactgct
     3121 acgcctcgcc ctatctccag gcacgcccgg gaagtcccca ctgctacgac gtgtgtgatc
     3181 ccggcaccat caatccggaa ctgggggcgg cggaggagtt cgaggccttc gtctcggctc
     3241 tcaaggagcg ggacatgggg ctgctgctgg atgtggttcc caaccacatg agcagttggg
     3301 ctaccggcaa cagactctgg cgcgacgtcc tcgagaacgg acccagttcc ccgtacgccc
     3361 gctttttcga cgtcgattgg cacccccttc ggagcgacac gctgctggag gaccgtgtct
     3421 tgctgccgat cctgggcgac tcctacggaa cggtgctgga gaggcaggaa ctgcaactct
     3481 tcttcgaaga cggtgcgttc cagcttcgct actacgagaa cctcctcccc ctggacccca
     3541 gcagctacat cgagatcctc cgcctctgcg aggagcgatt gtccgactcg atggatgtcg
     3601 gcgaagacaa tcttcgggaa ctgcagagca tcatcaccgc catttcgcac cttccgagcc
     3661 gcacagagcg agatcccgag cgtgttcagg agcgccagcg ggagaaggag gttgtcaagc
     3721 gcaggctgaa cgcgctctac gaggcgtctg ctcaggtagg ggctgccgtg aatgaggcgg
     3781 tgggcctttt caacggcatt tcgggagatc cttccagctt cgaccggctg gatgcgttgc
     3841 tggaacagca gccctatcgg ctcgctcact ggcaggttgc cgccgaggag atcaactacc
     3901 gccgtttctt cgacgtgagc gacctggtcg ccctacgcca ggaggacccc gaggtgttcg
     3961 acgaaacaca caggttgatc ctcaagctgt tgggtgaggg aaccgtgacc gggcttcgca
     4021 tcgaccacgt agatgggctg tgggaccctg cggcctacct gtcgcgcctc cagcgctcct
     4081 acctcgagga gttgggaagt gtgcgggcca ctgaggaaga ctccggtcgc cgcctctacc
     4141 tggtggtgga gaagatcctc cgaccggggg agaagcttcc gcccgattgg cctgtggacg
     4201 gaactacggg ctacgacttc gccaatgcgg tcaacggcat cttcgtcgac aactccaacg
     4261 ccaggtcgtt cgacgtcctg taccgccgct tcatcggttt cgacatggat ttccaggagc
     4321 agttgatcgc ggctaagaag agggtgatgt tgacctcgct ggccagcgaa gtgaacatgc
     4381 tggcttacca gttgcggcgg atcgcagccc gaaaccggaa ctaccgtgat ctgaccctca
     4441 acggcctcac cttcggactt cgggagacca tcgcctgcct acctgtgtac cgcacctaca
     4501 tctcccgcgg cgaccgtgag ttgcgagagc gcgacaggac ctacctggag ggggctctgg
     4561 cggaggccag gaggcgcaat ccgagcgccc ctgctgcagt gttcgacttc gtcgcggaga
     4621 tactcctgct gcgctggccg gcgggagtaa aggacgagga tcgcggacag ttgtgggatt
     4681 tcgtgaggga cttccagcag gtcaccggcc cgaccatggc caaggcgatg gaggacaccg
     4741 tcttctacgt ctacaaccgc ctcatctctc taaacgaggt gggtggggac ccgggaaggt
     4801 ttgggatctc ggtcgatgac tttcaccaac agaacgccct gagactgcag gaatggccat
     4861 actcgctcac ctccaccact acccacgaca ccaaacggag tgaaggggtg cgagcccgga
     4921 tcaacctcct ctctgagatg cccagggagt ggaaggcaat gctggggcgg tggagcaagt
     4981 ccaatgggcg gcgaaagagg actgtttccg gccggccggt gccggatcga aacgaggaat
     5041 actacctcta ccagacgctg ctgggtagct ggccggttgg aggtatggat ggggaacagt
     5101 accgcagcta tcaggaacgg gtggaggctc acatgatcaa ggcgatcagg gaggctaagg
     5161 tgaacacgag ttgggtcaat cccaactctc agtacgagga tgccgtccgg cacttcgtcg
     5221 gttccatctt gaaccagcag agggccaacc cgtttctcaa gagcttccag ccgtttcaac
     5281 tgtcgatcgc gcgttacggc atggtcaact ccctgtccca aacccttctg aagatcgctt
     5341 cccccggggt gcccgacctt taccagggga cggagctatg ggatctgtat ctggtggacc
     5401 ccgacaaccg gcgcccggtg gagtatggcc cgcggctcga aatgctggag cggatacagg
     5461 aggggcgtga aacgggtgag aggctctccg acctggccgc ggagttgctg gcacactggg
     5521 aggatggtgg tgtgaagctg cacctcatcc acagagccct gagctaccgg cgccgccatc
     5581 cggatctgtt tacccgtggt gactacgtgc ccctggcggc cacaggtcga catcgcgatc
     5641 gagtctgtgc attcgcccgg cgagatgagg atgcgaccgt cgttgtagtc gttccgaggc
     5701 tcgtgtgggg actgatgcag ggaaaggcgg acaagccggg cgtggatggt gggggacagt
     5761 ggctgccact tggggagggg atctgggaag atacccggct cctgctgccc tccgactggg
     5821 cggccggcgg tttcaggaac ctcctcacag gagaggagct tgagaccgta gagacccggg
     5881 gccgagaacg ttcctgtccg gattgcggcc acacaggtag gcaggcgatg gtctcccgaa
     5941 gtccgaaggt gaggccgttg agggtcagat cacggtagtt ccggtttcgg gctgcgatcc
     6001 gccgcaactg gtaagccagc atgttcact
//
LOCUS       DATALS010000048         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_229418, whole genome shotgun sequence.
ACCESSION   DATALS010000048 DATALS010000000
VERSION     DATALS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229418"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..890
                     /locus_tag="VHS06_00945"
     CDS             129..890
                     /locus_tag="VHS06_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX2986732.1"
                     /translation="MDNALALLVSVLGIVVIDLVLSGDNAVVIGMAARRLSPHQRRRA
                     IVLGGAAAIVLRITFAAIATLLLRVPLLQAAGGGLLLWIAWRLLREEQEEHELHESRS
                     MLGALRTIVLADVVMSLDNILAVAGASHGSLELLAFGLVLSMPLLLFGSSMVARIMNR
                     MPWLALVGAAILTWTAGGMIAEDEFLLSYLPYDHAIELLLPGLFTIGLVGPSFLQLPS
                     VARVLDPLLERMLRVAPLAPVVATMRRVGGKRIER"
     gene            1007..2650
                     /locus_tag="VHS06_00950"
     CDS             1007..2650
                     /locus_tag="VHS06_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX2986733.1"
                     /translation="MNGSAAIANILKREGVECLFCFPVNGVIDAAAEQGIRPIMARHE
                     RTVVGMADGYSRVTNGRKIGVCTTQYGPGAENAFGGVAQAFADNTPILFLPGGVYQNK
                     LGSEPGFDTLLSYQKVTKWIARVNQQERIPELFRRAFTYLRNGRRGPVALELPMDLLA
                     GEFGDAEPEYTPVDGCRTMADPARVAAAVKLILAAKRPLLHAGLGVLYAEATPELVEL
                     AELLQAPVMTTLPGKSGFPEDHPLSIGSGGLTGPGAVAHYLAESDLVFGIGSSLTTIF
                     VAAPIPAGKTIVQLTVDDRDLSKDYPAGCVLMGDAKLVLQQMAEEVKRQLGPDGKRGD
                     DRMAKEIASVKARWLDEWMPRLTSNETPLTPYRVIWELQQTLDLRNSVITHDAGTPRD
                     QISPFWPALEPNSFMGYGKSTHLGYSLPLALGAKVAKPEKTVVNFMGDAAFGMSGMEI
                     ANAARNRIGILTILLNNSCLGGYDKHIPNASRIYGTRFIPGVEYARAAEAMGAYAERV
                     EKPDEIAPAIRRALKVTSDGRPALLEMITKEENQLSKYW"
BASE COUNT          488 a          895 c          923 g          477 t
ORIGIN      
        1 agcgctccgg ccgatggagg acgatcccag tcgttccagg gccagacctt ccacgacgct
       61 tgtgcgtgga gggcctggct tcttttttgc gaccctctcg cacggaacga tgaaggagag
      121 tgacacaatt ggacaatgcc cttgccttac tggtcagcgt tctagggatc gtagtcatcg
      181 acctcgtgct cagcggggac aatgccgtgg tgatcggcat ggccgccagg cggctatcgc
      241 cccaccaacg ccgccgagcc atcgtcttag gcggtgccgc cgccatcgtc ctcaggataa
      301 ccttcgctgc tatcgctacc cttctgctgc gcgtccccct tctgcaggct gcaggcggtg
      361 ggctgctcct ctggatcgcc tggaggttgc ttcgggagga gcaggaggag catgagttgc
      421 acgagagcag atccatgctg ggggcgctgc gcaccatcgt gctggccgac gtggtcatga
      481 gcctggacaa catcctggcg gtggccggcg cgtcccacgg gagcctggaa ctgctcgcct
      541 ttggcctcgt cctcagcatg ccgctgctgc tgttcggcag cagcatggtt gcccgaatca
      601 tgaaccggat gccctggctg gcgttggtgg gcgccgccat tctcacctgg acggcaggcg
      661 ggatgatcgc cgaggacgag ttcctcctct cctacctgcc ctacgaccac gccatcgagc
      721 tgttgctgcc cggcctcttt accattgggc tggttgggcc atctttcctt cagttgcctt
      781 cggtggcgcg ggtgctcgac ccgctccttg agcggatgct cagggtggct cccctggccc
      841 ccgtggtcgc caccatgcgc cgggtcggcg gcaagcggat cgagaggtaa ctccaagcca
      901 aatgtcgaga ccgtttctcc caattgcttg caacagcatg tatattggtg atgatcgaat
      961 ccgccgggtt tcggcgaact gctgaagctg gaagggagaa acggtcttga acggatcagc
     1021 cgccatcgcc aatatcctga agcgcgaggg ggtggaatgc ctattctgct tcccggtcaa
     1081 cggtgtcatc gacgccgccg cggagcaggg gatccgcccc atcatggccc ggcacgagcg
     1141 cacggtcgtc gggatggccg acggctacag ccgcgtcacc aacggccgca agatcggcgt
     1201 atgcacgacg cagtacggtc cgggcgcgga gaacgccttc ggtggggtcg cccaggcgtt
     1261 tgccgacaac accccgatcc tcttcctgcc gggcggcgtc tatcagaaca agctgggctc
     1321 ggagcccggc ttcgacaccc tgctcagcta ccagaaggtc actaagtgga tcgccagggt
     1381 aaaccagcag gagcggatcc ccgagctgtt ccgccgcgct ttcacctacc ttcgcaacgg
     1441 ccggcggggg ccggtggcgc tggagttgcc tatggacctg ctggccggcg agttcgggga
     1501 tgcggagccg gagtacacgc ccgtggacgg ctgccgcacg atggccgacc ctgcgcgggt
     1561 cgccgccgcc gtcaagctga tccttgccgc caaacgtccg ctgcttcacg ctgggctggg
     1621 cgtcctatac gccgaggcga cgcccgagct ggtggagctg gctgagctgc tccaggcgcc
     1681 ggtgatgacc accctacccg gcaagagcgg cttcccggag gaccaccccc tctccatcgg
     1741 cagtggcggt cttaccggtc cgggggccgt ggcccactac ctggcggaat cggacctggt
     1801 cttcggcatc ggctccagtc tgaccactat cttcgtcgcc gccccgatcc cggccggcaa
     1861 gaccatcgtg cagctgaccg tggacgacag ggacctgagc aaggattacc cggccggctg
     1921 cgtcctgatg ggcgacgcca agctggtgct ccagcagatg gcggaggagg tgaagcggca
     1981 gcttgggccg gacggcaagc ggggcgacga ccgcatggcg aaggagatcg cctcggtcaa
     2041 ggccaggtgg ctggacgaat ggatgccaag gctcacctcc aacgaaaccc cgctcacccc
     2101 ctatcgggtg atctgggagt tgcagcaaac cctggacctg cgcaacagcg tcatcaccca
     2161 cgacgcgggg accccgcggg atcagatatc tcccttctgg ccggcgcttg agcccaactc
     2221 cttcatgggc tacggcaagt cgacccatct gggctacagc ctgccgctgg cgctcggggc
     2281 gaaggtggcg aagccggaga agacggtggt caacttcatg ggcgacgcgg cgttcgggat
     2341 gagcggcatg gagatcgcca acgcagcgcg aaaccggatc ggtatcctca ccatcctgct
     2401 gaacaactcc tgcctgggcg gctacgacaa acacatcccc aacgccagcc ggatctacgg
     2461 gactcgcttc atccctgggg tcgagtacgc tcgggcggca gaggcgatgg gtgcctacgc
     2521 cgagagggtg gagaagccgg acgagatcgc tcccgccatc cggcgtgcgc tgaaggttac
     2581 ctcggatggc cgcccggccc tgctagagat gatcaccaag gaggagaacc agctctccaa
     2641 gtactggtag gagacgcggg gacacggaga cgcggggagg aaggccctgg gatcgcgggc
     2701 cgaagctccg cgaagagcga acgaacagga tggacaggat aggcaggatg ggattcgatg
     2761 agaagccgcc cgccgtcttg agg
//
LOCUS       DATALS010000049         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_364343, whole genome shotgun sequence.
ACCESSION   DATALS010000049 DATALS010000000
VERSION     DATALS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364343"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VHS06_00955"
     CDS             <1..706
                     /locus_tag="VHS06_00955"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2986734.1"
                     /translation="RYNRKLLIIAGDVLIASFTLVLAITFLAGYRELWLLFLISGIRS
                     AGAGIQTPAVSAFIPQIVPPERLMRVSGLNGSIQAILMIVAPAASGALLAFSAIEAVL
                     FVDVATAAAAVLIMVTLKAPPHPKEGLPTSTGYLDALVGGLQYVRQTPWVRTLLIYYA
                     LFMFLVTPAAFLTPLLIARTFGGEVWQLTANEMLFGGGAVLGGAIIAAWGGFKNRMHS
                     VALASVTFGVLTAALG"
     gene            complement(711..989)
                     /locus_tag="VHS06_00960"
                     /pseudo
     CDS             complement(711..989)
                     /locus_tag="VHS06_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1029..1658)
                     /locus_tag="VHS06_00965"
     CDS             complement(1029..1658)
                     /locus_tag="VHS06_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986735.1"
                     /translation="MKRLLLVATMLMVALLSAGCKMSINMETTVLPSTEMDQSVIITY
                     EGLPEEAMQNVKSEETALAENGWTQEVTTEGDTVVMRATRHLQYGDDLFPPTEGGADS
                     PKPRYDLQVVDTPEYREYSLSILMQSDESAASPTSAETAVDPEMAEFGAEIAEMMTDM
                     MEFSWTINMPGEVVDTNADVRTENGGTWVFGQDQAKDRELKMVSRERKQ"
BASE COUNT          307 a          591 c          526 g          415 t
ORIGIN      
        1 caggtataac cgcaagctgc tcatcatcgc cggcgacgtt ctaatagcat ccttcaccct
       61 tgtcctcgcc atcacctttc tcgctggata cagggagctc tggctgctgt tcttgatctc
      121 cggcattcga tctgccggcg cggggataca gactccagcg gtcagtgcct tcattccgca
      181 gatcgtgccg ccggaaaggc tgatgcgggt gagcgggctg aatgggagca ttcaggcgat
      241 actgatgatc gtggcgccgg ccgccagcgg cgcactgctc gcattttcgg ccatcgaggc
      301 cgtgctcttc gtcgacgtcg ccaccgccgc ggcggccgtt ctcataatgg tcaccctcaa
      361 ggcaccgcct cacccgaagg agggccttcc caccagtacc ggctatctgg acgctctggt
      421 tggcggcctg cagtacgtcc ggcagacccc gtgggtcagg acattgctca tttactacgc
      481 cctgttcatg ttcctggtca ccccggctgc cttcctcacc cccctgctga tcgcccgcac
      541 gttcggaggg gaggtgtggc agctcaccgc caacgagatg ctgttcggcg gcggagccgt
      601 cctgggaggg gcgatcattg ccgcctgggg cggcttcaag aaccgcatgc acagtgtggc
      661 gctggcaagc gtcacgtttg gagtcctgac ggccgcactg gggtgaagga tgctattaga
      721 acgtcgccgg cgatgatgag cagcttgcgg ttatacctgt ccgcccagac cccggcgatc
      781 ggcgatatgg cgatctgcgg caggaacccg cagaccgtgg aaacggtcat catggcgccg
      841 gactgcgtac tcagggttat gtaccagatg atcgcgtact gcaccagcga cgagccgaac
      901 agcgacaggg tctggctggc gataaacagc gtcgttctct ttgcccagcc cgcgaactga
      961 tccgtgcggc catcggtata gttggtcatc gactccccta gatcaagctg tctacccact
     1021 cggcctcgct actgcttgcg ctcgcggctg accatcttca gctctcgatc cttcgcctgg
     1081 tcctgtccaa agacccaggt gcccccgttc tctgtgcgga cgtcggcgtt tgtatccacc
     1141 acttcacccg gcatgttgat cgtccacgag aactccatca tgtcggtcat catttcggct
     1201 atctcggcgc cgaattcggc catctctgga tcgactgcag tctcggcgga agtgggtgat
     1261 gcggccgatt cgtcggactg catcaggatg ctcaggctgt actctcgata ttcaggggta
     1321 tccaccacct gtagatcgta cctgggcttg ggcgagtccg cgccgccctc agtcggtgga
     1381 aacaggtcgt caccatactg cagatgcctg gtcgctcgca tgacgaccgt gtctccctcg
     1441 gtcgtcacct cctgggtcca cccgttctct gccagcgcgg tttcctcgga cttcacattc
     1501 tgcatcgctt cttcggggag cccctcgtac gtgatgatca cactctgatc catctccgtg
     1561 gagggtagca ccgtggtttc catgttgatg gacattttgc atcccgcgga gagcaaggcc
     1621 accattagca tcgtcgccac cagtaatagc cttttcacct ctacctcctc tgcaacctgc
     1681 cggttctcag ggtgcttgag gcgccgtcca cgctacggtc gtggtagagc ctcgtgtccg
     1741 ttcactccgg caggagccac tttgctgttc attattagac caggttggcg gctcggaggt
     1801 caaaaggcat tggccttaga cgctgtgacc atcaccttc
//
LOCUS       DATALS010000050         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_364504, whole genome shotgun sequence.
ACCESSION   DATALS010000050 DATALS010000000
VERSION     DATALS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364504"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..904)
                     /gene="cax"
                     /locus_tag="VHS06_00970"
     CDS             complement(59..904)
                     /gene="cax"
                     /locus_tag="VHS06_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX2986736.1"
                     /translation="MARNRRDMPPSVVQGRVGMVAELVGFLGESWINWLLVLVPVSWA
                     VELLDLPRLWVFLFSALAIIPLAHLIGHGTEQVAGWIGHGPGGLLNAAMGNATELIIS
                     LFALKAGLITVVKAAISGSLIGNTLLVLGMSMLVGGWGRDKQTFNRTRAGATASMLFL
                     AVVALVMPAVFDLEVFGTLRHPAASVDSLSLLVCVVLILTYMASLVFSLKTHREVIPT
                     VHTECDQPRLSLVNSVTLLLAATVLVAVESELLVGGISDATAALGMTEFFVGIVVVAV
                     VGNAE"
     gene            1019..1564
                     /locus_tag="VHS06_00975"
     CDS             1019..1564
                     /locus_tag="VHS06_00975"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012597863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX2986737.1"
                     /translation="MREITINGKEVRLVQGDITTCSVDAIGNAANSGLRGGGGVDGAI
                     HRAGGPTIMEECRKIGGCPTGEARITTAGRLPARYVIHAVGPIYRDGKHGEPELLASA
                     YRWSIKLAAEKGLESIALPSISTGVYGYPLSEAAPIALRTVVEECRKPSSLRSITFVL
                     FNRETMEAYEATLEQIARELA"
     gene            1699..>2771
                     /locus_tag="VHS06_00980"
     CDS             1699..>2771
                     /locus_tag="VHS06_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986738.1"
                     /translation="MTLFTDQESTSTRTIWIDSLRQQLTRGVTTRVSGVPFLARRARL
                     ETSQNAGSYVKCELGDQTGWIDAIWWDAGKMDRADLQSILVTAAWIVDGVASLNRFGN
                     RETPQIKIERVRIDGTDPLDIPGLVRRSVLSDEELRQWLSDTIATIRNRPLRSLLEDN
                     LGEGSCWRREYLRVPAGLHYHHAYLGGLADHVREMAEVWQSSVSTFPRVDRDLTLGGI
                     LLHDIGKLDTFSSGAMPQIAESGRYMDHITAGIGRLTPGHGREARLPAPVARPPAAHP
                     GLPPRRKGARIPRRTCNSGGHRGSPSGSDEQPAEPSGRVDPPIRDRPIRLVHRCIPLA
                     EDQRGPRHHYPRRFPATRRAGLNL"
BASE COUNT          578 a          885 c          837 g          471 t
ORIGIN      
        1 caggtagtga tatccccctg aacgagtctg acctctttgc cgttgatggt gatctctctc
       61 attcggcgtt cccaacaact gccaccacga cgatgccaac gaagaactca gtcatgccca
      121 gcgcagcagt ggcatcggag atgccgccaa ccaacagttc ggattccacg gccaccaata
      181 cggtggccgc caacaacagc gtcaccgagt tgaccaggct cagcctcggc tggtcgcact
      241 cggtgtggac ggttgggatc acctccctat gcgtcttcag cgagaagacg aggctggcca
      301 tgtaggtgag gataagcacg acacagacca gaaggctgag agagtccacc gaggctgcgg
      361 ggtgcctgag ggtgccgaac acctccaggt cgaacaccgc aggcatgacc agggccacga
      421 cggcgagaaa tagcatggag gccgtcgctc ccgccctggt acggttgaag gtctgcttgt
      481 ccctgcccca cccgccgacc agcatgctca ttcccagcac cagcagcgtg ttgccgatga
      541 ggctgccgct gatcgctgct ttgaccacag taatgaggcc ggccttcaga gcgaagaggc
      601 tgataatcaa ctcggtggcg ttccccatag cggcgtttag gagacccccc gggccatggc
      661 cgatccaacc ggcaacctgc tccgtcccat ggccgatcag gtgcgccagt gggatgatcg
      721 ccagtgcaga gaacagaaag acccacagac gagggaggtc cagcaactca accgcccaac
      781 tcacaggaac cagcaccagc agccagttga tccagctctc accgaggaag ccgaccaact
      841 ccgccaccat ccccacgcgg ccttgcacca cactgggtgg catgtcccgc ctgttgcgtg
      901 ccatagcgcg tctggtgcgc aagccccatg ccggaagctt gaaccaacat gctctcaggg
      961 gtaatctcta ttcgagtgag aggtcatagt cgcgaaacga gaagggtgga aacgccggat
     1021 gagagagatc accatcaacg gcaaagaggt cagactcgtt cagggggata tcactacctg
     1081 cagtgtggac gccatcggca acgccgccaa ctcggggctg cgtggtggcg gcggggtaga
     1141 cggcgctatt cacagggcag gtgggccgac gatcatggag gagtgcagga agatcggcgg
     1201 ctgccccacc ggtgaggcaa ggattaccac ggcgggaagg ttgcccgcca ggtacgtgat
     1261 ccacgcggtc ggcccgatct accgggacgg caagcacgga gagccggaac tgctggcatc
     1321 ggcctatcgc tggtcgatta agctggccgc cgagaagggg ctggaaagca tcgcccttcc
     1381 ctccatcagc accggggtat acggctaccc gttgagcgag gcggcaccaa tagcgctccg
     1441 cacggtggtg gaggaatgcc gaaagccgag ttcgctgcgc tccatcacgt tcgtcctgtt
     1501 caaccgggag accatggagg catacgaggc cacgctcgag cagattgctc gtgagcttgc
     1561 ctgatccgtt gactgcacac ccataggcct gccatactca gccaggcggt agtagttgct
     1621 cgccggcaat tgactgccgc tgggcgccgc ttccccttag tgccgttcca cgcccgattc
     1681 tgcaccgaga ggcaaaccat gacccttttc accgaccagg aatccaccag cacccgcacc
     1741 atatggatcg actcgctcag gcagcagctg acgcgtggag tcaccaccag ggtgtcgggg
     1801 gtcccattcc tggcacgaag ggcgcggttg gagaccagtc agaatgccgg aagttacgtc
     1861 aagtgcgagc tgggggatca gaccggctgg attgacgcca tctggtggga cgccggcaag
     1921 atggatcgtg ctgatctgca gtcgattctg gtaacggcag cgtggatcgt ggatggtgtc
     1981 gcatccctga atcgcttcgg caaccgcgag accccacaga tcaaaatcga acgggttcgc
     2041 atcgacggca cagatcccct cgatataccg gggcttgttc gacggagcgt cctgtccgat
     2101 gaggaactgc gacagtggct gtccgacacc atcgccacca tccgaaaccg tccgctccgc
     2161 tcgctgctgg aagacaacct gggagagggc agttgctggc ggcgcgagta ccttcgcgtt
     2221 cccgcggggc tccactacca ccacgcctat ctaggggggc tggccgacca cgtgcgcgag
     2281 atggcggagg tgtggcagtc ctcggtatcg acctttcccc gagtcgatag ggatctgact
     2341 ctgggaggca tcctcctcca tgacatcggc aagctggaca ccttctcctc cggcgccatg
     2401 cctcagatcg ccgagagtgg tcgttacatg gatcacataa ccgcgggcat tgggagactg
     2461 acccctggcc atggacgcga ggcacgactt cccgcccctg ttgcgcgacc acctgctgca
     2521 catcctggcc tcccaccacg gcgaaaagga gcacggatcc cccgtcgtac ctgcaactcg
     2581 ggaggccatc gtggttcacc atctggatcg gatgagcagc ctgctgagcc atctggacga
     2641 gtggacccgc caatccggga tcgaccaatt cggctggtcc accgatgcat ccccctggct
     2701 gaagaccagc gtggccctcg ccaccactat cccagacgat ttcctgccac ccgacgagca
     2761 ggcctgaacc t
//
LOCUS       DATALS010000051         7483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_4668,
            whole genome shotgun sequence.
ACCESSION   DATALS010000051 DATALS010000000
VERSION     DATALS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4668"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /gene="hemB"
                     /locus_tag="VHS06_00985"
     CDS             <1..621
                     /gene="hemB"
                     /locus_tag="VHS06_00985"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX2986739.1"
                     /translation="CLCEYTSHGHCGVVERGQVLNDPTLELLSRTALSHVEAGADMVA
                     PSDMMDGRVGAIRRTLDAAGHESVPIMAYSAKYASAFYGPFREAADSAPQFGDRRGYQ
                     MSPTNAREALREVELDIQEGADIVMVKPALAYLDVIARVRDAVKLPVAAYNVSGEYSM
                     VKAAARLGWLEEKRAAMETLTAIKRAGADIIITYFAKDLVRWLQEG"
     gene            890..1123
                     /locus_tag="VHS06_00990"
     CDS             890..1123
                     /locus_tag="VHS06_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2986740.1"
                     /translation="MIETGFPSNASARPTNWVEINLKWCKSCSICVAFCPTKALSADD
                     VGTPHLANPEKCTACGLCEMMCPDFAIEVIKKR"
     gene            1120..2274
                     /locus_tag="VHS06_00995"
     CDS             1120..2274
                     /locus_tag="VHS06_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX2986741.1"
                     /translation="MTEERPKPILLQGNEAIAEGALAAGMRFFGGYPISPSSEVAETL
                     ARRLPQVGGTFIQMEDEIASISSIIGASIAGVKSMTATSGPGYSLMQEGLGFAAITET
                     PCVIVNVQRMGPSTGVATAPAQADVMQARYGTHGPHPMIALSPSTVKECFDVTVKAFN
                     LSEKYRTPVVILADAVVGHLREVVTLPRPDEIEVVNRMRPDGPPGTIQPFAVADGDGV
                     PTVPDFGTGYRFHVTGLFHDETGFPTTNPVKVQATTERLVNKVEAHTDELTEVEYLYM
                     DDAEVVVVSFGCSGRSSRAAVKAARARGIKAGLVRLITIWPFPEKLIQAIVEKTPKIV
                     VAEMNLGEVAGEIRRVTRNEVQVTQVNRHDGMLLTTNQVLSGIEGVMQHA"
     gene            2267..3097
                     /locus_tag="VHS06_01000"
     CDS             2267..3097
                     /locus_tag="VHS06_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2986742.1"
                     /translation="MPNALVSKYLRVNRLPQMWCPGCGIGEIVNGVIRAIDKVGLDQD
                     KTAIVSGIGCSSRATGYMNFDTLHTTHGRALAFATGLKLAKPELNVVVIMGDGDCSAI
                     GGNHFIHASRRNIDVTAIVVNNQIYGMTGGQYSPTTPMDFRATTAPYGDAEPGFDLCA
                     LAQAAGATFVGRAAPARMPDLINTIAKALQHKGFSFVETVSQCPVYFGRMNRLGGPVQ
                     MLMKQKEITVPVRVAQQNPEEAKGKLVTGVLYEGERPAYETLYQQVRLKASQKAGEHV
                     "
     gene            3090..3653
                     /locus_tag="VHS06_01005"
     CDS             3090..3653
                     /locus_tag="VHS06_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX2986743.1"
                     /translation="MSKTVEVSLGGSGGQGLVLMGLILAEAAGIYEGHKVVSSQLYGP
                     ESRGGASRSDVIISDEEIDYPEVENPDILFVFNQAAADKYISHLKQGGTVIYDSTFVT
                     KLPEAKGKVFHLPITQLAREKVGRDIVANIVAIGAVVTITGIVSRDSLIQAVMDRVPK
                     GTEEVNRKALQVGFDAAEALMSQANPA"
     gene            3990..5120
                     /gene="sucC"
                     /locus_tag="VHS06_01010"
     CDS             3990..5120
                     /gene="sucC"
                     /locus_tag="VHS06_01010"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX2986744.1"
                     /translation="MKLYEFQAKRVFAQGGIPVPQGEVVQDPEDAARVAERLGRVVVK
                     AQVHVGGRGKAGGIKLANTPEEARQYAAGMLGKPLKGFTVNYALVEEAQNIAAEYYLG
                     ITVDRNAEKAIVMLSSMGGVDIEEVAATQPAKIARLHVDPSWGVWDFQLRDLVARADL
                     DPKVSRDIVSVIRKLYNVFVSSDASLAEINPLMVTADGKVVAADGKFDVDDNALFRQK
                     ELLKYREVSEADPIEAEAARLGVAYVRLDGNIGVIGNGAGLVMTTLDILSRNGGKAAN
                     FCDLGGGAKAETVRQDLGLVLMNPNVKGVLFNIFGGIVRCDEVAKGIIEATRTMEINV
                     PIVIRMSGTKAAEGRELLKGTNLIPAATAQEAAQKIIELVKG"
     gene            5213..6085
                     /gene="sucD"
                     /locus_tag="VHS06_01015"
     CDS             5213..6085
                     /gene="sucD"
                     /locus_tag="VHS06_01015"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX2986745.1"
                     /translation="MSILINKDTRVVVHGITGREGAFHSQKMIEYGTQVVAGVTPGKG
                     GAVQDGVPVFESAAEAVKATGANTSIIFVPAPFAADSAVVAAEAGVKLVVVITDGIPT
                     LDMIRVVNYIRPKGVRLIGPNCPGIISPGEAKVGIMPAEVYREGPIGVISRSGTLTYE
                     TCDHITNMGMGVSTCIGIGGDPIIGTRFLDALKLFREDDETRVVVLIGEIGGSDEEMA
                     AAYIRESRYPKPVVAFISGRSAPPGKRMGHAGAIISGGTGTPQAKVKAFEEAGVPVGN
                     TLEEVVQLAKERLG"
     gene            complement(6082..>7483)
                     /locus_tag="VHS06_01020"
     CDS             complement(6082..>7483)
                     /locus_tag="VHS06_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986746.1"
                     /translation="PTVSARPFPPDEDSAAPSASTFTQPTDGEEDIPLRSRYEAIRDC
                     TLRRYKQEEKHNPIQQSPLGQILARTPLWESRLALANWPLMDEAEDLDLGNFSFPISP
                     YTPVADDGVQRFLLLSNSSIIANFRQWDYLERAHRRMFDRAVWAMDSGRTGVATVEFD
                     RGLTDQKVDYMVRMLAGYGVKTIIWGNEPNSPYAPWRDNLPDLFAIFSAAANARRQHA
                     LSDLELAMPGLAYFGQGEYLQKMVRTFKELQKKSGAPSWAGEIPADRIVDHYYGPVDD
                     FLPRIKMMRDIMSKEGVGKLKYDLMEVGNPTMDSSQQPASDEQMAECHIPQVAGLAVG
                     SGLVDRFCYYSLLGSNWEDSITVVNDGRILRRPPYQAFVNMARLLARLDRAILTDAND
                     MVRLEGARSDGIDFTVLWSKVTDRELQVAVTKGKRVFTALGQEVKGERPDQVVLAPKQ
                     HPLLAGPARILIAGGS"
BASE COUNT         1436 a         2104 c         2538 g         1405 t
ORIGIN      
        1 tgcctctgcg agtacaccag ccacggccac tgcggcgtgg tggaacgggg acaggtgttg
       61 aacgatccca ccctggagtt gctgtcccga actgccctct cccacgtgga ggcgggcgcc
      121 gacatggttg ccccctcgga catgatggac ggtcgcgtcg gtgcgatccg ccgtaccctg
      181 gacgccgccg ggcacgagtc ggtgccgatc atggcctaca gcgccaagta tgcctctgct
      241 ttctacgggc cgttccgtga ggccgccgat tccgcgccgc agtttggcga tcggcggggc
      301 tatcagatgt ctccgaccaa tgcgcgtgaa gcgctgcggg aggtggagct cgacatccag
      361 gagggggccg acatcgtgat ggtgaagcct gcgctggcct acctggacgt gatcgcccgg
      421 gtgcgggatg cggtgaagct gccggtggct gcctacaacg tgagcggtga gtattcgatg
      481 gtgaaggccg cggcgaggct ggggtggctg gaggagaagc gagccgcgat ggagaccctc
      541 acggccatca agcgggcagg tgcggacata atcatcacct atttcgccaa ggatctggtt
      601 cgctggctcc aggaggggtg acgcgaagac acggggacgc ggagagtgct ggagcgaggt
      661 ggcaggggcc gataccacaa ctgcacagtg gctggggtgt tgggtggatc tctgtcggca
      721 gtctgttgat ggtcggaatc gagaatggat ttgagccagc gtgtactggg tgatgggcat
      781 tgcatcttcg tgacagagtg agtgaagctg cttgaccccc tcaagggccg atgctagcat
      841 ttctttagag gtgcactcac cccggtagga acctaagaaa ggatcgccga tgatcgaaac
      901 aggttttccc tccaacgcct ctgcgaggcc gacgaattgg gtggagatta acctcaaatg
      961 gtgtaaatcc tgctcgatat gcgtcgcctt ctgccccacg aaagcgttat ccgccgatga
     1021 tgtcggaacg ccccatcttg ctaatccgga gaagtgcacc gcctgcgggc tgtgcgagat
     1081 gatgtgtccg gactttgcca tcgaggtgat caagaagcga tgaccgaaga aagaccgaag
     1141 cctatcctgc tacaaggcaa tgaggccata gccgagggag cgctcgcagc gggcatgcgg
     1201 ttcttcggcg gctaccccat cagcccgtcc tcggaggttg cggagacgct cgcgcgtcga
     1261 ctcccacagg tgggcgggac attcatccag atggaggatg agatcgcaag catatcctcc
     1321 atcatagggg cctccatcgc cggcgtgaaa tccatgacgg cgaccagcgg tcccggttac
     1381 tccctgatgc aggaggggct cgggtttgct gctatcaccg aaaccccctg cgtgatcgtc
     1441 aacgtgcagc gaatgggccc cagcaccggg gttgccaccg cgcctgccca ggcggacgtg
     1501 atgcaggcca ggtacggcac ccacggacct cacccgatga ttgccctctc gccgtcgacg
     1561 gtaaaagagt gctttgatgt gactgtcaag gcgttcaatc tctctgagaa ataccgcacc
     1621 cctgtggtga tcctcgccga tgctgttgtg ggtcacttga gggaggtggt cacactcccc
     1681 aggccggacg agatcgaagt ggtgaatagg atgcggcccg acggccctcc cggcaccatc
     1741 cagccttttg ccgtggccga tggtgatggc gttccaacgg tacccgattt cgggaccggc
     1801 tatcggttcc acgttaccgg gctgttccac gatgagacgg gtttccccac caccaatccg
     1861 gtaaaggttc aggccacaac ggagcggctc gtgaacaagg tggaagccca cacggacgag
     1921 ttgaccgaag tcgagtacct gtatatggac gatgccgagg tggttgtggt ttccttcggc
     1981 tgctccgggc gctcatcccg tgccgcggtc aaggcggctc gggccagggg gatcaaggcc
     2041 ggcctggtgc ggctcatcac gatctggcca ttccctgaga agctcatcca ggcgattgtc
     2101 gagaagacgc cgaagatcgt cgtggcagag atgaacctgg gagaggtggc cggcgagatc
     2161 aggcgtgtca cccgcaacga ggtgcaagtg acgcaggtca accggcacga tgggatgctg
     2221 ctcactacca atcaggtact cagcgggatc gagggggtga tgcagcatgc ctaacgcatt
     2281 ggtgagcaag tatctacgcg ttaaccgact tccccagatg tggtgccccg gttgtggtat
     2341 cggcgagatt gtcaacggcg tcatccgggc catcgacaag gtaggcctgg atcaggacaa
     2401 gaccgcaata gtatcaggca tcggctgctc cagccgcgcc accggataca tgaacttcga
     2461 tacccttcac acgacccacg gtcgagcgct ggccttcgcc actggactga agctggcgaa
     2521 gccggagttg aacgtggtcg tgatcatggg cgatggcgat tgctctgcga tcggcggcaa
     2581 ccatttcatc cacgcttcgc gccgcaacat agacgtcact gcgatagtgg tcaataatca
     2641 gatttacggt atgacgggcg gccagtactc ccctacaacg cctatggact tcagggcgac
     2701 gacggcgccc tatggggacg ctgagccggg gtttgacctg tgtgcgctgg cgcaggctgc
     2761 cggcgcgacc ttcgtcggca gggcggctcc ggcgaggatg cccgatctga tcaacaccat
     2821 cgcaaaggcg ttgcagcaca agggcttctc cttcgtggag acagtctcgc agtgccccgt
     2881 gtacttcggc cgaatgaacc ggctgggcgg gccggtgcag atgctgatga agcagaaaga
     2941 gatcacggtg ccggtgcggg tggcacagca gaaccctgaa gaggccaagg gcaagttggt
     3001 taccggggtc ctatacgaag gggagaggcc ggcctacgag actctatacc agcaggtgcg
     3061 gctgaaggcc tcgcagaagg caggtgagca tgtctaagac agtagaagta tccctgggtg
     3121 gctccggcgg tcaggggctg gtactgatgg gactgatcct ggccgaggcc gcgggaatat
     3181 acgaaggcca caaggtggtc tccagccaac tctacggccc cgagtcgcgc ggtggggcat
     3241 cccgctcgga cgtgatcatc agtgacgagg agatcgatta ccccgaggtg gagaaccccg
     3301 acatcctgtt tgtcttcaac caggcggccg cggacaagta catctctcac ctcaagcagg
     3361 gtgggacggt gatatacgac tccacctttg tgaccaagct gccggaggcc aagggtaagg
     3421 tcttccatct gccgattacg cagcttgccc gagagaaggt gggtagggac atcgtcgcca
     3481 atatagtggc gatcggagcg gtcgttacca tcacaggcat cgtgtcccga gactccttga
     3541 tccaagcagt gatggacagg gttccgaagg gcactgagga ggtcaatcgg aaggcgcttc
     3601 aggtcggctt cgatgccgcc gaggcgttga tgagccaggc aaacccggct taactatata
     3661 ggcatagcat ctaaaggcac cagcggtttc caggaacgcc cccatttccg gtgcgggtgc
     3721 gatctatgca gatgaagcag gtagaggttt ctgcatagat cgcgcccgcg gcggctagtc
     3781 tcgagaggca ttctagcgag cctgtctggt gcctttctcg ttatgacggg gatgcatcgt
     3841 ggtttgtcgg gccgtgatca atgggtggtg ggggtggacc tgtgtgtctg cccaggccga
     3901 ggggggacac accggtccgc ccctaccgat gtttcgggta ttgggcgcgt tattctgttg
     3961 ccaaaccgtc agtctggggg agaacagaat tgaagctgta cgagtttcag gctaagcggg
     4021 tgtttgccca gggtgggatc cccgtgcctc agggcgaggt ggtgcaggat cccgaggatg
     4081 ccgcccgggt cgccgagagg ctgggtcggg tggtggtgaa ggcgcaggtg catgtgggcg
     4141 gccggggcaa ggccggcggc atcaagctgg ccaacacccc cgaggaggcg aggcagtacg
     4201 ccgccgggat gctggggaag ccgctgaagg gcttcacggt gaactatgcc ctggtagagg
     4261 aggcgcagaa catcgccgcg gagtactacc tggggatcac ggtggaccgg aacgccgaga
     4321 aggcgatcgt gatgctcagc agcatgggcg gcgtggacat cgaggaggtc gccgccaccc
     4381 aacccgccaa gattgccagg ctgcacgtcg acccttcctg gggggtgtgg gatttccagc
     4441 tgcgcgacct ggtggctcgg gccgacctgg atccgaaggt ctcccgcgac atcgtctcgg
     4501 tgatccgcaa actgtacaac gtcttcgtct cgtcggatgc ctctctggcg gagatcaatc
     4561 cgctcatggt gacggccgac ggtaaggtgg tggcggccga cggcaagttc gacgtggacg
     4621 acaacgccct cttccgtcag aaggaattgc tgaagtaccg ggaggtctca gaggccgatc
     4681 ccatcgaagc cgaggccgca aggctgggag tggcctacgt gaggttggat gggaacattg
     4741 gggtgatcgg aaacggcgcc ggtctggtga tgaccacgtt ggacatcctg agccgcaacg
     4801 gcggaaaagc cgccaacttt tgcgacctgg gcggcggcgc caaggcggaa acggtgcgtc
     4861 aggacctggg gctggtgctg atgaacccca acgtcaaagg ggtgctgttc aacatttttg
     4921 ggggcattgt ccggtgcgac gaggtggcca aggggatcat cgaggcgacg cgtaccatgg
     4981 agatcaatgt gcccatcgtg atacggatgt ccggaaccaa ggcagccgag gggcgtgagc
     5041 tgctgaaggg caccaacctg atccctgcag ccaccgcgca ggaggctgcc cagaagatca
     5101 tcgagctggt gaagggctga atgacggtag atcgttctgg gttctgagtt ctgagtgaag
     5161 agaacccaga actcagaacc cagaaccgag taccgaccga cggaggatag tgatgtcaat
     5221 actgattaac aaggacactc gcgtcgtggt gcacggcata acgggacgtg agggcgcctt
     5281 ccacagccag aagatgatcg agtatggcac ccaggtggtt gcaggggtga cccccggcaa
     5341 aggtggtgcg gtgcaggacg gcgtgcctgt gttcgagagc gcggccgagg cagtgaaggc
     5401 caccggtgcc aacacgtcga tcatcttcgt ccctgccccg tttgcggcgg actctgccgt
     5461 ggtagcggca gaggctggcg tgaagctggt ggtggttatt accgacggca tcccgacgct
     5521 ggacatgatc cgtgtcgtca actacatcag gccgaagggg gttcggctga tcggccccaa
     5581 ctgccccggc atcatcagcc cgggtgaggc gaaggtgggc atcatgccgg ccgaggtgta
     5641 ccgggaagga ccgatcggcg tgatctcccg cagcggcacg ctgacctatg agacttgcga
     5701 ccacataacc aatatgggca tgggagtctc gacctgcatc gggatcggcg gcgacccgat
     5761 catcggcacc cgcttccttg atgcactgaa gctgttccga gaggacgacg aaaccagggt
     5821 cgtggtgctg atcggggaga ttgggggctc ggacgaggag atggccgccg cctacatcag
     5881 ggagagccgc tacccgaaac cggttgtggc cttcatctcg gggaggtcgg cgcctccggg
     5941 caagcggatg ggccatgcgg gggccatcat ttccggcggg acagggaccc cgcaggccaa
     6001 ggtgaaggct ttcgaggagg cgggggtgcc ggtgggcaac acgctggaag aggtggtgca
     6061 gctggcgaag gagcgactcg gctaggaacc gccggcgatc aatatcctgg cgggcccggc
     6121 caggagggga tgctgcttcg gtgccagcac cacctggtcg ggcctttctc ccttcacctc
     6181 ctggccgagc gcggtgaaga ccctctttcc cttggtgacc gctacctgga gttcccgatc
     6241 ggttaccttg gaccagagta ccgtgaagtc gatcccgtcg ctcctcgccc cctccagcct
     6301 caccatgtcg ttcgcatcgg tcaggatggc cctatccagc ctggccagga gcctggccat
     6361 gttgacgaat gcctggtagg gggggcgcct caggattcgg ccgtcgttca caacggtaat
     6421 ggagtcctcc cagttgcttc ccagcagcga gtagtaacag aagcggtcga ccagccctga
     6481 gccgactgcc aggccggcga cctgaggaat gtggcattcg gccatctgct cgtcggaagc
     6541 cggttgctgg ctgctatcca tggtggggtt gccgacctcc atcaggtcgt acttcagttt
     6601 gcccacccct tcttttgaca taatgtcgcg catcatcttg atgcggggca agaagtcatc
     6661 taccgggccg taatagtgat cgacgatccg gtccgcgggg atctcacctg cccagctcgg
     6721 ggcaccgctc ttcttctgca gctccttgaa ggtgcggacc attttctgga ggtattcgcc
     6781 ttgcccgaag taggcaaggc ccggcatggc cagctccagg tcggagaggg cgtgctgcct
     6841 tctcgcgttg gcggccgcgg agaagatagc gaacaagtct ggaaggttat cccgccaggg
     6901 tgcgtagggg ctgttgggct cgttacccca tatgatggtc ttgacgccgt atccggccag
     6961 catccgaacc atgtaatcca ccttttggtc ggtcaggcca cggtcgaact ccacggtcgc
     7021 gacccccgtg cggccgctgt ccatcgccca cacggctcgg tcgaacatcc tgcggtgtgc
     7081 acgttcgagg tagtcccact gccggaagtt ggcgatgatg ctgctgttgg acagcaggag
     7141 aaagcgttgc acgccgtcat cggcgaccgg cgtgtagggt gagatgggga aggagaagtt
     7201 ccccaggtcc aggtcctcgg cttcatccat gaggggccag ttggcgaggg ccaggcggga
     7261 ctcccagagc ggggttctgg ccaggatctg tcccaggggt gattgctgta tgggattgtg
     7321 tttctcttcc tgcttgtacc ggcgtagggt acagtccctg atggcctcgt acctggatcg
     7381 aagaggaata tcctcctctc cgtcggtcgg ctgggtgaag gtggatgcgc ttggggccgc
     7441 gctgtcttcg tccggcggga aggggcgcgc ggagaccgtg ggg
//
LOCUS       DATALS010000052         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_47395,
            whole genome shotgun sequence.
ACCESSION   DATALS010000052 DATALS010000000
VERSION     DATALS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47395"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /gene="queG"
                     /locus_tag="VHS06_01025"
     CDS             <1..854
                     /gene="queG"
                     /locus_tag="VHS06_01025"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX2986747.1"
                     /translation="PRGRIARYALGDDYHDVLLSRVRQLLDAVSSSLGTRPAGRFFVD
                     SSPLSERAVAARAGLGWFGKNSCLLTSRGSWMLLAEIVTDLELQPDEPVRRDCGRCRI
                     CLDRCPTGALVGPYRLDARRCISYLTIEHRGAIPADLRPLIGDRIFGCDVCQEVCPHN
                     RLARPCDEQAFAPRAGVGASPALLPLMSMDGARFKAMFRGSPVLRTKRRGLLRNVAVA
                     LGNSGDPAAVPALSAGLGDPEPLVRGHAAWALGRIGGIQARSALERALAAEADDEVRR
                     EIIAALA"
     gene            909..2273
                     /locus_tag="VHS06_01030"
     CDS             909..2273
                     /locus_tag="VHS06_01030"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX2986748.1"
                     /translation="MANLEEFISENEVRYLDELKQFLRIPSISALPEHKQDVERCARF
                     AADHMLSIGMQRAEALPTAGYPVVYGEWMGAPGRPTVLVYGHYDVQPADPVELWASPP
                     FDPQVRGNELFARGSADDKGQVYMHWAAVDAHMKSNGRLPVNIKFLIEGEEEVGSDNL
                     DTFIQDHRDLLKADLVVVSDTGWFDKGVPSITYGLRGITYVQVDLKTADTDLHSGSYG
                     GAVANPIQVLAEMIAGLKDSKGRITIPGFYDRVRPLSPEERRQFESLPFDAEGFRKSI
                     GAKELWGEEGYTVLERIWARPALDINGIWGGFTGEGSKTVLPARASAKISCRLVPNQD
                     PQEIARLLEARLRELCPPTVEMEFRTLNTGRGAITPIDHPAVQATARAMERAYGKKPA
                     FIRSGGSIPVVASFAEFLGIPSVLMGIGLPDERSHAPNEHLDLGNFFGGIRAAAFLWE
                     ELSK"
     gene            2332..>2741
                     /locus_tag="VHS06_01035"
     CDS             2332..>2741
                     /locus_tag="VHS06_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2986749.1"
                     /translation="MKSSRRGELEAYAMIALAATMWGGLGVIAKWLYADGMDPWALIF
                     FRTSMGALAFGGVLLIGDREWWRVRREDLPFLALYGLISTSAFYALYLFTISLTSVAA
                     AAVLLYTAPSMAVVMAHFAFGESITRPKIAALLLS"
BASE COUNT          443 a          820 c          935 g          543 t
ORIGIN      
        1 ggccgagggg gcgaatcgcc cgctatgctc tgggtgacga ttaccacgac gtcctccttt
       61 ccagggtgcg gcagcttctg gatgcggtct cctcctccct cggcacgcgc cctgccggac
      121 ggttcttcgt cgacagttcg ccgctctcgg agcgggccgt ggcggcacga gccggattgg
      181 gctggttcgg caagaacagt tgccttctta ccagcagagg ctcctggatg ctgctggccg
      241 agatcgtaac cgacctggaa ttgcagcccg acgagccggt ccgccgcgac tgcggtcgct
      301 gccggatttg tctggatcgt tgccccaccg gagcgctggt ggggccgtat cgcctggacg
      361 cccggcgctg tatctcatat ctgactatcg agcaccgagg ggctatccct gccgacctgc
      421 gccctctcat cggggaccgg atcttcggct gcgacgtctg ccaggaggtc tgtccacata
      481 atcggctggc gcggccatgc gatgagcagg cctttgctcc gcgagcgggg gtcggggcct
      541 cgccggcgct gctgccactc atgtcgatgg atggggcccg gttcaaggcg atgtttcggg
      601 gtagtccggt gcttcgtacc aagcggcggg ggttgctgcg aaacgtcgcg gtggcgctgg
      661 gtaactccgg cgatccggcg gcggtccctg ccctttcggc cgggcttggt gaccctgagc
      721 cgttggtccg cggccatgcg gcgtgggctc tgggtagaat cggcggcata caggcgagat
      781 cggcactgga acgagccctg gcggcggaag ccgatgatga ggtgcggcgg gagattattg
      841 cggcgctggc gtgagcctgt tccccgcgtt gtccgtccgt attgctagtg tggaaggagt
      901 gtcctgcgat ggcaaatctt gaggaattca tctcggagaa cgaggtccgg tacctggacg
      961 agttgaagca gttccttcgc attcccagca tcagcgccct tccggagcac aagcaggacg
     1021 ttgaacggtg tgcccgcttt gccgcggacc acatgctctc cattggaatg cagcgggcgg
     1081 aggcgttgcc cactgcgggg tatccggtgg tctatgggga gtggatggga gcgccgggcc
     1141 gccccacggt cctggtgtac ggccactacg acgtgcaacc cgccgatcct gtggagttgt
     1201 gggccagccc gcccttcgat ccccaggttc gcggcaacga actgttcgcc agggggtcgg
     1261 ccgacgacaa ggggcaggtc tacatgcact gggcggccgt ggatgctcac atgaagtcga
     1321 acggcagact gccggtgaac ataaagttcc tgatcgaggg cgaggaggag gtcggcagcg
     1381 acaatcttga taccttcatt caggatcacc gcgacctgct caaggctgac ctggtcgtgg
     1441 tgtccgacac tggctggttc gataaagggg ttccctcaat cacctacgga ctcaggggca
     1501 taacctacgt acaggtggat ctgaagacgg ccgacacgga tcttcactcc ggttcctatg
     1561 gcggcgccgt ggccaaccca atccaggtgc tggcggagat gattgccggg ctgaaggaca
     1621 gcaagggacg tatcaccatc cccggcttct acgatcgggt tcgccccctg tctccagaag
     1681 agaggcgcca gttcgagtcg ctacccttcg atgccgaggg cttccgcaag agcattgggg
     1741 cgaaggagct ctggggcgag gagggctaca ccgttctgga gcgaatttgg gcgcgtcccg
     1801 cgctggacat caacggcatc tggggaggct tcaccggcga ggggagcaag actgttttgc
     1861 ctgccagggc gtctgcgaag atcagttgcc ggctagttcc gaaccaggac ccgcaggaga
     1921 tagcacggct cctggaagcg cggcttcgag aactgtgccc accgacggtg gagatggaat
     1981 ttcgcaccct gaacaccggc aggggagcca tcacgccgat cgatcacccc gccgttcagg
     2041 cgacggcccg ggctatggag cgggcgtacg ggaagaagcc ggccttcatc cgttcgggtg
     2101 gctccatacc ggtcgttgcg tcctttgctg agttcctggg catcccttcg gtgctgatgg
     2161 ggatcggcct tccggacgag cgcagccacg cgcccaacga gcatctcgac ctcggcaact
     2221 tctttggtgg gatccgcgcc gcggccttcc tctgggagga gttgagcaag tgagcaagcg
     2281 agtcgtctcg attctgttgg cggagttgat tgccagggag gcaccggcct gttgaaatca
     2341 tccagacggg gcgagttgga ggcatacgcg atgatcgccc ttgccgccac gatgtggggc
     2401 ggcctgggcg tgatcgccaa gtggctgtat gccgacggga tggatccttg ggcgctgatc
     2461 ttctttcgca catccatggg agcgctggcc ttcggcggcg tgcttctcat cggagacaga
     2521 gagtggtgga gggtgcgtcg ggaggacctg cccttcctgg cgctgtatgg gttgatcagc
     2581 acgtcggcct tctacgccct ctacctcttc accatcagcc tgacgagcgt ggccgctgcc
     2641 gccgtcttgc tctacactgc accttccatg gcggtggtca tggcgcactt tgccttcggc
     2701 gagtcgatca ccaggccgaa gattgcggct ctgctgctct c
//
LOCUS       DATALS010000053         6889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_229831, whole genome shotgun sequence.
ACCESSION   DATALS010000053 DATALS010000000
VERSION     DATALS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229831"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VHS06_01040"
     CDS             complement(<1..835)
                     /locus_tag="VHS06_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX2986750.1"
                     /translation="MATHSMTPGKEAWSRSLSRHMAGTWLKLALTVLATLAVLLLPPQ
                     PGLSPEGQRVLSIVAAAVVLWATEVIPVAVSSLLVVVLLMVMGTVKSPSQALVGFSSP
                     IVFFLVGSLAMGAAIMKSGLAERLAGYLLRRSGGDPKWLAAQLLLSMPFIAFVMPSAI
                     NRNSMLIPAYERVFQTLSVGKRDPLSKVIMLTLGLLNPFASSAFMTGGLAPMTTSTLL
                     GGFTWFRWFGLMAVPYYTLLALGGLMVYLIYRPPTSTGPSPEVASTPHKPLTRDEKVT
                     LA"
     gene            1194..1577
                     /locus_tag="VHS06_01045"
     CDS             1194..1577
                     /locus_tag="VHS06_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2986751.1"
                     /translation="MSERGFRVEEDEQNYRELADRLSSLPGLVETLPDMERRVARMVA
                     EGQRPYEIAMAEGISEQAVWEMVRSLANVTSGRATTRGYETSGLGADTDAGVTGGYGD
                     TGFGSIGTEGGEPVTEEPEEGSEQE"
     gene            complement(1663..2688)
                     /locus_tag="VHS06_01050"
     CDS             complement(1663..2688)
                     /locus_tag="VHS06_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2986752.1"
                     /translation="MRIGILGPISWRIPPRHYGGWELVTHNLVEGLVRRGHDVTLFAT
                     EDSSTSAKLASICPKPLSEDSTLSARLYESLHTALTFEHAGEFDLVHNHLGCYPVCYS
                     RVIPVPLVTTLHGSAAEGHSRVIYNRYRDSNYVSITDAERILAPELNYIATVYNGIDV
                     AAFDFNPTPGDYLLVLGRMSPDKGIHSAIAVAQRLGMRLILAGIVPPENQEYFDTQVK
                     PQLQEGFIDYVGPADHKQKNRLLREAYAFLHLIGYHEAFGLTMVESMACGTPVIAVPM
                     GSVPEIVIHGETGFFANNVEEVCRLLPRIAELDRMRCRQLAEERFNVDQMVDGYVKVY
                     RRVLGAE"
     gene            2850..3488
                     /locus_tag="VHS06_01055"
     CDS             2850..3488
                     /locus_tag="VHS06_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2986753.1"
                     /translation="MDVAAHTSEKDGQKTQEEVQELVRRACGGDAEAFGVLYDTYLDS
                     VYRYVFYKVGDATLAEDLTAQLFMKAWEAMPRYQLREIPFSHWLMRLARNAVIDYYRT
                     SRVQGELDEGLVSREPDPLGEYLQGERVRGLQRALQRLPEEHRTVIVLRFIEEMDYAQ
                     VAEIVGKSQGALRVIQHRALQTLRRILQREGEEGVSDDRGGPGRMSREGGAR"
     gene            complement(4659..5747)
                     /locus_tag="VHS06_01060"
     CDS             complement(4659..5747)
                     /locus_tag="VHS06_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX2986754.1"
                     /translation="MGKKSFASLALATTVGLAVLATACGGGSAPAPTQAPSKPAATSA
                     PAATAPASATKPAGTSAPAASGAPALTKEYKLSMATGGTAGTYYPYGGAIAGLWSKNL
                     KGVNVTAETTGAAVENQRLLGNKQVELALTQNDVADYAMNGTEMFKEKITNFRAVAAL
                     YPEVLQWVVTPNIKTISDLKGKKFVVGAAASGTEANSRQVLETLGMSYKDLGKAAFLS
                     YAEAASAMKDRQMDGFAVTGGVPTSAITDVTTAQEISLLPISGDDAKKVMDKYKFFVP
                     VKVPASAYKGLTGEVQTIAVQSMLVARDDLDADLVYWLTKTLIEKQADLAQSHAKGKD
                     LSKESAVKGLTVPLQGTLCFPLVCEFFH"
BASE COUNT         1516 a         1935 c         2088 g         1350 t
ORIGIN      
        1 cggccagcgt aaccttctca tccctcgtga gcggcttgtg cggggttgag gctacttcgg
       61 gggatggtcc ggtcgaggtt ggtgggcggt agatcaggta gaccatcaga ccaccaagcg
      121 ccagcagcgt gtaatagggc acggccatca gtccgaacca ccggaaccag gtaaaccctc
      181 ccagcaaggt agaggtggtc atcggtgcca aacctcccgt catgaatgcc gaggaggcaa
      241 atgggttgag caatcccagt gtgagcatga tcaccttgga tagggggtct cgcttgccga
      301 ccgagagcgt ctggaacact ctctcgtagg ccggaatcaa catggagttg cggttgatcg
      361 ccgagggcat tacgaaagcg atgaagggca tagacaagag caactgtgct gcgagccatt
      421 tgggatcccc tccggatctt ctcagcaggt agcccgctaa cctctcggcc aagccggact
      481 tcatgatcgc ggcgcccatc gctaacgatc caaccaggaa gaagacaata ggagaggaga
      541 atccaaccag cgcctgcgaa gggcttttga ccgtgcccat caccattagg agcaccacaa
      601 ccagcaaaga gcttaccgca actgggatga cctctgtagc ccagaggacg acggcggcgg
      661 cgacgatgga cagcacgcgc tgcccctcgg gggagagtcc gggctggggt ggcagcagga
      721 gaactgcgag cgtggcgagc accgtgagtg ctagtttgag ccaggtgccg gccatgtgtc
      781 tggagaggct acgcgaccag gcttctttgc ctggcgtcat cgaatgtgtt gccaagactg
      841 tacctccgga aaaccggaca gtgatcagca tccgctgaca ggctctcggc gttcataaca
      901 cgccatacga ttgtgcagct ttacccagga ttgcatccac ggtagcacag cagcacaacg
      961 cgcgacaatg ttgaaggtag tatggcatgc atcctgcctt agcagcggtg gcgttccgtc
     1021 cgcaatgggg ttaccgtcct gtgggggagg tggtgcgggc gtatgcaatc tttaatcacc
     1081 tcgatagggt cgcttggttg ggttgcctcc actgagcggc ccatcttgtt gactccgata
     1141 gctgtgcagc gagtgaccga caccagggaa gcctgtggca gggaagagga gacatgagcg
     1201 aacgtggttt cagggtagag gaagacgagc agaactacag ggaactggcg gaccgtctga
     1261 gcagcctgcc tggcctggtg gagacgctgc cggatatgga gcgacgcgtt gcccggatgg
     1321 tggccgaggg ccagaggccc tacgagatcg cgatggccga agggatcagc gagcaggccg
     1381 tctgggagat ggtcagatcg ctggcgaacg tcaccagtgg tcgtgccacc acgcgaggat
     1441 acgagacatc aggccttggg gccgataccg acgccggtgt gactggaggc tatggggata
     1501 ccggtttcgg atccattggc accgaggggg gcgagccggt caccgaggag cctgaagagg
     1561 gttccgagca ggaataacgc ctcatcctga ccctctccca tgggagaggt aagtcttcta
     1621 caccctctcc ctctgggaga gggtcggggt gatggaaaca gcttactcgg cccctaacac
     1681 tctccggtaa actttcacgt acccatccac catctgatcc acgttgaacc gctcttccgc
     1741 caactgtctg caacgcatcc tatccaactc ggcgatcctt gggagtagac ggcagacttc
     1801 ttcgacgttg ttggcgaaga atcccgtttc tccgtggatc acgatctccg gcaccgaacc
     1861 cattgggacg gcgatgacgg gtgtcccgca agccatggat tccaccatcg taagaccgaa
     1921 tgcctcgtga tagccgatca gatggagaaa tgcgtatgcc tcacgtagca ggcggttctt
     1981 ctgtttgtga tcggcgggac ccacgtaatc gatgaagccc tcctgtagct gcggtttcac
     2041 ctgagtatcg aagtactcct ggttctcggg cggcacgatg cctgccagga tgaggcgcat
     2101 ccccaaacgc tgggcgacgg cgatggcgga gtgtatcccc ttgtctggcg acatcctccc
     2161 cagcaccagc aggtaatccc ccggcgtagg attgaaatcg aaggcggcga cgtcgatgcc
     2221 gttgtagacg gtggcgatgt agttcaactc tggcgccagt atgcgctcag catcggtgat
     2281 ggagacgtag ttggaatcac gataccggtt gtagatgact cgactgtgcc cctcggcggc
     2341 cgagccatgc agcgtagtga ccagcggcac tggaatcacc ctgctatagc aaactggata
     2401 gcagcccagg tggttgtgga ctagatcgaa ctcgccggca tgctcgaaag tgagggcggt
     2461 atggagcgac tcgtacaacc ttgcggagag ggtagaatcc tcagagaggg gcttaggaca
     2521 gatgcttgcc aacttggcgc tggtcgagga atcctcggtt gcgaagagag tgacatcatg
     2581 ccctcgcctc accaacccct ccaccaggtt gtgggtcaca agttcccacc ctccgtagtg
     2641 tcttggggga atgcgccagg agatggggcc aaggatgcct atccgcatcg acgacctcct
     2701 cgatgcgcat ccactgcaag gttcaagcca gacatctgga tagtactatt tgcgcataga
     2761 cgtatcgctt cacctcaaca tagactgaca cattaactat ggaggcacct tctttttcct
     2821 tgtaacacct ctctcctcag gacgttctta tggatgtagc ggcccacaca tccgagaaag
     2881 acgggcagaa gactcaagaa gaagtacagg aattggtacg gcgggcttgt ggtggggacg
     2941 ccgaagcctt tggcgtgctg tacgacacct acctggactc tgtctaccgg tacgtctttt
     3001 acaaggtggg cgatgcgacc ctggctgaag atctcacggc tcagctcttc atgaaggcat
     3061 gggaggcgat gcctcgttat cagttgaggg agataccctt ctcccactgg ctgatgaggc
     3121 tcgcaagaaa tgcggtgatt gactactaca ggacttcgag agtccaggga gagctggacg
     3181 agggactggt atcgcgtgaa cccgatcccc tgggcgaata ccttcagggc gagcgggtgc
     3241 gggggctgca gagagccctg cagcggctcc cagaggagca tcgcacggtc atcgtgttac
     3301 ggttcatcga ggagatggac tacgctcagg tggccgagat tgtgggcaag agccaggggg
     3361 cgctgagggt tatccagcat cgggccctgc agacgctaag acggattttg cagcgagagg
     3421 gcgaagaggg tgtctcggac gatagaggcg gtcctggccg gatgtctcga gaaggtggag
     3481 cgcgatgagt gctcgatcga agaggctctg gctgggtatc cagagttcag ggaggagttg
     3541 gagcctctcc tgcgacttgc catcgagcta cgctccttgc ccaaggttac cgcaccggct
     3601 agcctgagga gctacaaccg tcctgtcttc caatctcatg ccaacgtcat ctctctgcca
     3661 tcccaccgat ggtggcatcc gctagccaga cccagttcct ctttggcgcg cattgctgca
     3721 gggatcgttg tctgcttcct gctgctcggt agcagtgttg tggcctcggc cagtagcctc
     3781 cccgaggagc ctctgtatcc ggtgaagctg gctgttgaag gggttcagct tgccctggcg
     3841 ccagacgacc agtcgcgttc catattggag atggagtttg cctccaggcg gttgcaggag
     3901 gtcgagagcg ccgcgagcca gggaagagcc gacgcgatcc agaaagggct cgagctgtac
     3961 caggagaagt tggagggcgt gctgggttcg gtgcaaatgc tctctgagga ggagtcacag
     4021 cagctccaga catcgttggg gcggcagcag gagacattgg ccagggttta cagacaggtt
     4081 cctgagaatg ctcaacccgc gatcttgaag gcgatgcagg catccaagcg gggacaggcc
     4141 gagactgaaa agccaggggc ggagcccgct ggtaagccca cagggacgat cactcctggt
     4201 tcggccgata aatcaaccac aacgcaggat cgctccaacc cggctgctgc cccgtcaggg
     4261 gaacaggagc ccaggggcaa gacggagcct gccgccgtac ccactcccaa agaccaaccc
     4321 ggcaaggctc gagatggcag aagcagcgag gggagcgacg aaaacagcca aaaggctgag
     4381 agacccgggc aaaatggaca gcagagagga cgcacgagtt ccgtctcccc atcggtgtcg
     4441 atagacgatg tctccacggt tgctttagaa acagctacag ctgttccttc atcacagcaa
     4501 atcgaagtaa cgcctgtcgc ggctggagat caatcgcacg acctcagcag taatcgggag
     4561 tccaagcaga gggatgttcc agtagccact cccgagacac cggttcttgc ctccgtggcc
     4621 gtagtgccca cgatgccact acttcccacc ctgcgttctc aatggaagaa ctcacagaca
     4681 agtgggaagc agagtgtccc ctgcagcgga acagtaaggc ccttgaccgc agactccttg
     4741 gagaggtcct tgcctttggc atgtgactga gccaggtcag cctgcttctc gatcagggtc
     4801 ttggtcagcc agtagaccag gtcggcatcg agatcatcac gcgcgaccag catcgactgg
     4861 acagcgatgg tctgaacctc gccggtaagg cccttgtaag cgctggcagg aaccttgacc
     4921 ggcacgaaga acttgtactt atccatcacc ttcttggcat cgtcgccact gattggcagc
     4981 agcgagattt cctgagcagt ggtgacatca gtgatggccg aggtaggaac accaccggtg
     5041 acggcaaatc catccatctg ccggtccttc atagcagagg cggcttcagc gtaggatagg
     5101 aatgccgcct tccccagatc cttgtagctc atgccaaggg tctcgaggac ctgcctggag
     5161 ttcgcctctg tgcccgaggc ggcagcgccg accacgaact tcttgccctt caggtcagag
     5221 atggtcttga tgttgggggt aacgacccac tgcagcactt caggatagag ggcggcgacg
     5281 gcgcggaagt tggtgatctt ctccttgaac atctccgtgc cgttcatcgc atagtcggcc
     5341 acatcgttct gggtcagcgc cagctcaacc tgcttgttgc ccagcaaccg ctggttctct
     5401 accgcagccc cggtggtctc ggcggtcacg ttcacgccct tcaggttctt ggaccagagt
     5461 ccagcgatgg cgccgccgta gggatagtag gttccagccg ttccgccggt ggccatggag
     5521 agcttgtact ccttagttag agcaggggca ccagaagcgg caggagcgct agtgccggcc
     5581 ggcttggttg cagaagcagg agccgttgcc gcgggtgcgg aggtagccgc tggcttagat
     5641 ggggcctgag tcggggcagg cgcagaaccg ccgccgcaag ccgtcgccag cacagcaaga
     5701 cccaccgtcg tggcgagagc gagagacgcg aaagacttct ttcccaacac ggttttaatt
     5761 tcctcctaat ctaccaatac tgggggccaa ttggtggcat cacacgggaa acccgccatc
     5821 gccgcgcccg atcgcaagtc ccctttcaag caactcccag agacgcggcg tttcgtcctc
     5881 tggcagtacg ctggacaccc aaaagtttgg atttgttgaa ggcattgcca atcttcaaca
     5941 gagtttggat ggaagcggag gagacaccaa caccgggagc tgtcgctgca aaagccacct
     6001 ttagcattgc cgacacgaag cgaaacggag ctggcatcct tcgtatcacg actccttctc
     6061 tttcatccag aatacaaaat gcagccgccc aggtcgcaac catcaactta gctgaagctg
     6121 ctacaccacc tccttccagc cacaatcttt gccggaatcg gtatacggcg cgatacggat
     6181 agctatcctt gccgcgcctc gcagctaaca cacgtcaaag agactgttgt tatgccaggt
     6241 tacccatgtg gaaatccaac aacattcggc ctctccgtcg acctgtcaca ggaatcacag
     6301 accgatgatt tgttggtcag tcaggtcaca atcgaccact ggggtttcca gaatcgccat
     6361 ggatccagga ctaccgagcc aaaccattgt tgaacgatcg acacaaccag gacggaatcg
     6421 ctcggccact atcggggaat acactgctca cggggaaatg cagcagtgta agcaaccatg
     6481 aaccatatgt acagataact gcttgcttta cctggctgtc aagtttagcg atggcctact
     6541 cgcttgtcaa taaagcgatc caccattata ggagccgcga tggagacgca agcaggtgtg
     6601 caagaagcgc cattgaagga acaccaggca gcgattcctg catacagacg atcttatctc
     6661 tgagagtaaa gtgaatgcca gtgaggaaga gttgtttatc tccgccgacg tcctacttgc
     6721 ggttgttgtc cgaccggccc tgctctggcc gaccagatga tttcgctgcg gggacactct
     6781 gcttcccact tgtctgtgag ttcttccatt gagaacgcag ggtgggaagt agtggcatcg
     6841 tgggcactac ggccacggag gcaagaaccg gtgtctcggg agtggctac
//
LOCUS       DATALS010000054         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_185042, whole genome shotgun sequence.
ACCESSION   DATALS010000054 DATALS010000000
VERSION     DATALS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185042"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1039
                     /locus_tag="VHS06_01065"
     CDS             179..1039
                     /locus_tag="VHS06_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986755.1"
                     /translation="MVRQRCEALDHRARQCGKPGARLVRNQGWLCKDHDPDRLNPPLG
                     QLCAAEGCGAPAENYDDSFGLWYCPQHTVEQWRGPCTALVQTLYPCAREAEDLYHFED
                     DCGHDPHMWLCERHHWQYTQPGGPKRELVLLDWQSLGYPEMQDSSDGKEPLEDPDGID
                     GVIDWERRESDDDLIRFVLSHDTGRMWEIRYAPSGRATGARIVTEGSDTWTPVDWQSF
                     GIDAIEADEDAEGLRGYVSFERLDGDGGVVLHIYSQRSNRLWQLHLNSDGVVDSVLRA
                     PDDLAAEDIC"
     gene            complement(1156..1878)
                     /locus_tag="VHS06_01070"
     CDS             complement(1156..1878)
                     /locus_tag="VHS06_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX2986756.1"
                     /translation="MKVLAINGSPHCEKGNTALILGPFLEGMRQAGAEVDLAYSWKMK
                     VNPCGSDWSCLARTAGRCIHDDDMRDLYPKLREADILVIAAPVYASGMPSSLKSVVDR
                     MVPLSQPFIELNDGHCFHPLVEGAKGGKVVLVANCGFWELDNFDPMVAQVKGMSRSMG
                     REYAGALLRPHGSSFAMMLRMGAPVGGVLDAAREAGRQLVETGDMSAELQLAVSRPVI
                     PLEEFVRGNNDALRQMLVASTR"
BASE COUNT          412 a          659 c          627 g          368 t
ORIGIN      
        1 ggtgttttac gaagtgcccg tgcacgcctt tcatgacggc aacggggacg gcgtgggatg
       61 gaaggtgggg ggcgccggtt gccggttcga gagcaccgcc caggagtgga gatcgcggat
      121 cccaccggac tgtccatcga acttacctaa gtggtagaag gacggagaga acagttcaat
      181 ggtgagacaa cgctgcgaag cgttagatca tagagcccga cagtgcggaa aaccgggggc
      241 aaggctggtc cgaaaccagg gctggctgtg caaagatcac gacccggaca ggctgaaccc
      301 tcccctgggc caactctgcg cggccgaagg ctgcggtgcc ccggcagaga actacgacga
      361 cagcttcggc ctgtggtact gcccacagca cacggtggag cagtggagag gtccttgtac
      421 ggcgttggtc cagacactct acccctgtgc cagggaagcc gaagacctct accacttcga
      481 ggatgattgc gggcacgacc cgcacatgtg gctatgcgag cgccaccact ggcagtacac
      541 gcaaccgggc ggccccaaac gggagttggt cctgctggat tggcagtcgc tcgggtaccc
      601 ggagatgcag gattcctctg acggaaagga accgttggag gatcccgacg gcatagacgg
      661 tgttatcgat tgggagcgaa gggagtcgga cgacgatctc atccgtttcg tgctcagcca
      721 cgataccggg cgaatgtggg agatccgata cgccccatcc ggccgcgcga ccggagcgag
      781 aatcgtcacc gagggatcag acacatggac accggtggac tggcagtcat ttgggattga
      841 tgcgatcgag gctgacgaag atgcggaagg tcttcgaggg tatgtgagct tcgagcggct
      901 cgacggagac gggggtgtcg tcctgcacat atacagtcaa cggtccaaca ggctctggca
      961 acttcacctg aacagcgatg gagttgtgga ctcggtgctt cgggcgccgg acgacctggc
     1021 cgccgaggac atctgctagg ctccctcgcg aggcatgcgg caaaccgcag tgtggtgggg
     1081 tgctttggag tgcggcgtca agccgccgca ctccaaatgg ctccatctcc cgggctccac
     1141 gtcttcgtgt ctccactagc gagtgctcgc caccagcatc tgtcggagcg cgtcgttgtt
     1201 tccccgaacg aactcctcta gcgggataac cggacggctc acggcgagct gaagctccgc
     1261 ggacatatcc ccggtttcga cgagttgccg gccagcctcc ctcgccgcgt ccagaacgcc
     1321 acccaccggc gcccccatcc gcagcatcat cgcgaaactg gaaccatggg gccgcaacag
     1381 tgcccccgcg tactccctgc ccatgctcct gctcatcccc ttcacctgcg ccaccatcgg
     1441 atcaaagttg tccaattccc agaatccgca gttcgccacc aggactacct tgccaccctt
     1501 ggctccttcg acaaggggat ggaagcagtg tccgtcgttc agctctatga agggctggct
     1561 caacggaacc atccgatcca cgacgctctt tagtgaggaa ggcatcccgc tcgcgtacac
     1621 cggagcagca attaccagga tgtccgcctc gcgcagcttc gggtagaggt ctctcatgtc
     1681 gtcgtcgtga atgcaccggc ccgccgtcct ggccagacag ctccaatccg atccgcaagg
     1741 gttgaccttc atcttccacg aataggccag atcgacctcc gcgccggctt ggcgcatacc
     1801 ctccaggaat gggccgagga tcagggcagt gttccccttc tcgcagtgtg ggctaccgtt
     1861 aatcgccaga accttcatca cttcgtcctc cctcatggag cctaatcgcc gttctccgat
     1921 ctcaggactc ttacccccaa cagcggcagg ccaaggtcgc gcaccgcgat gagcagaccg
     1981 tagagtgcgg cctggtccgc gatcggcccc gctatgagcg tctccccgcc ggggccgcac
     2041 tcaaccgaca tcccctggaa ccagtc
//
LOCUS       DATALS010000055         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_5100,
            whole genome shotgun sequence.
ACCESSION   DATALS010000055 DATALS010000000
VERSION     DATALS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5100"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="VHS06_01075"
     CDS             complement(<1..1718)
                     /locus_tag="VHS06_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2986757.1"
                     /translation="MLITSVIPSLTIGDAIDSLEESLFVGRKREIAAFRRWLDSDSEP
                     EVLLSVTGRGGIGKSTLLKAFARIARQSGHTVIHLDSRDFVHTKDGLARALGDGCDGD
                     IASYLNSRQPLVLLDTFDELGDLVRYFRDGLLPRLRTGVKVVVAGRHIVRAGRGDHWG
                     GNPLVRSLELGVFDGKECREYLVRRGLTDPALMARVQRVTGGNPLAVSLAADMVLQLG
                     LREFTAAPEWHLVVRSLVDRLLNDVTDPSLRRLLEAASVMRQFDEAALAAVVGDEDIG
                     PAFARLCRMSVVRPMEHGLVLHDDVRRAIADDLRWRRSDRYDGMKLRAVAYYKERLNS
                     ATPTEREWLLAELLFLWEDALTHALVYNDPEPGMVWIDDARPEDYPDITRIWNWWRPN
                     VLDRAVGFEGELRGDSEFLEAMIRYPGCRVRVARDRDGTVCGFSTAIPVCQQSIPILR
                     IHSAFSPMIETYWSEEELAALPVSYEQSRIWFFVHLGYSETAVPEATQAALMRDHVGM
                     YAQGDVYIIVAPHPDIKTLMQAWGYERVPGAKNWNWGPHQAVEGFVLDLRSIGVERWI
                     ESILSG"
BASE COUNT          488 a          766 c          627 g          443 t
ORIGIN      
        1 tcgccgctca ggatggactc gatccatcgc tccacgccga tactcctcaa gtccagaacg
       61 aagccctcaa cggcttggtg gggtccccag ttccagttct tggcgccggg cacgcgctcg
      121 tacccccagg cctgcatgag cgtcttgata tcgggatgcg gggcgacgat tatgtagacg
      181 tccccctggg catacatgcc aacgtgatcg cgcatcagag cggcctgagt cgcctccggc
      241 accgccgtct cactgtagcc gaggtgaacg aagaaccata tcctgctctg ttcgtaactg
      301 actggcaggg cggccaactc ctcctcgctc cagtatgtct cgatcatcgg agagaatgcc
      361 gaatggatcc gcaatatcgg tatggactgc tggcatacgg gaatcgcggt gctaaaccca
      421 cacaccgtcc catcgcggtc ccttgcgacc cgcaccctgc accctggata ccggatcatc
      481 gcctcaagaa actcactgtc accgcgcaat tccccttcga agccgaccgc gcgatccaaa
      541 acgttgggtc tccaccagtt ccatatgcgg gtgatatcgg ggtagtcctc cggccgtgcg
      601 tcatcgatcc acaccatccc cggctcaggg tcgttgtaga ctaacgcatg ggtgagggcg
      661 tcctcccaga ggaacaacag ctctgccagc aaccattccc gctctgtcgg ggtggccgag
      721 ttcaagcgtt ccttgtagta ggccaccgcc cgcaacttca tgccgtcgta ccgatccgac
      781 cgtcgccagc gcaagtcatc cgcgattgcc ctccgcacgt cgtcgtggag aacgagaccg
      841 tgctccatcg ggcgaacgac ggacatccgg cacaagcgag cgaacgccgg accgatatct
      901 tcgtcaccta ccacggctgc caacgcggct tcatcgaact ggcgcataac ggaggccgcc
      961 tccagcagcc tcctgagcga tggatctgtc acatcattca gcaaccggtc aaccaacgac
     1021 cgcacaacta gatgccattc gggggccgct gtaaactccc gaagccccaa ctgcagcacc
     1081 atgtctgcag ccagggaaac cgccagtgga tttccccccg tcacccgctg caccctcgcc
     1141 ataagggcgg gatccgtcag gcctcgccgc acgaggtact cccggcactc cttgccatcg
     1201 aacacaccca gctcaagtga tcgcaccaga ggattgcccc cccaatggtc gccgcggccc
     1261 gctctcacaa tgtgccggcc tgcaacaacc acctttacgc cggttcgcaa cctcggcagc
     1321 aatccatctc ggaagtagcg gaccagatcg cccaactcgt cgaaggtgtc cagcaagact
     1381 agaggctgtc tgctgttgag ataggaggcg atgtccccgt cacaaccatc tcccaaagcc
     1441 cttgccagtc catccttggt gtgaacgaag tcgcgactat cgagatggat gactgtgtgc
     1501 ccggattgtc gagcaatccg ggcaaaagcc ttcaacagag tgctcttgcc gatccctccc
     1561 cgaccggtga cgctcaataa cacttccggc tcggaatccg agtccagcca tcgccggaaa
     1621 gcagcgatct ccctctttcg gccaacaaat agggactcct cgaggctatc gatagcgtcg
     1681 cctatggtga gcgagggtat tacactcgtg atgagcaagg gtcttacact ccaatgggca
     1741 taggtggcaa ctcctgccgg gatctcttcg gcaggtagaa catcgatgga gtggattaaa
     1801 aacctggaga caccatatcc agtagtttgc agttgttcca ggggatcgtc atccgagaga
     1861 atacgcatct tgaaagtgac agtcaaccac gcacgcacgc cgcgatgggt gagactactc
     1921 agagacggta ccccgactca gcccaaggtg gttccttagc atgcttcttg caaggaatct
     1981 ggaagaagga ttccacggtg cgtttccatg cacggcgatg ggcgaactgc cgccccatcg
     2041 ccttctgccc tgccgtagac agctcacgct gccaatatct cgcgggctcc gatgaaggcc
     2101 cgctttcacc acagacgtgg ggacatcgta gccctttccg gttgaggaag aagaggtttg
     2161 gcaacgaaca acggctgtgt aaccccttca gcagctaccc gatcctccgc actactccgt
     2221 cgctggctgc ccctggtgct gctagttcgc ccggggctgt tggctgccgg ggggcccttg
     2281 gggttcagag ccgattgggt gttgtcacaa ccggcggcca acag
//
LOCUS       DATALS010000056         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_139449, whole genome shotgun sequence.
ACCESSION   DATALS010000056 DATALS010000000
VERSION     DATALS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139449"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..990)
                     /gene="cbiB"
                     /locus_tag="VHS06_01080"
     CDS             complement(64..990)
                     /gene="cbiB"
                     /locus_tag="VHS06_01080"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEX2986758.1"
                     /translation="MRRGLVIVLALGIDLLFGELPNRWHPVAAMGRLVGKLEARAPGK
                     GALRQFIYGGMVESICLTAAIVPSRIVQRLLPARSLVGALAEAVALKPTFALRDLFLA
                     TDRVRRPLEQGDLEGARQAVGQLVSRDVSALDESEVAAAAVETLAENASDSVVAPLLL
                     YAAFGLPGAYGYRMANTLDAMIGYRGRYEYTGKVAARLDDLLNLLPSRLTALATVIAA
                     RPVGTSPADAWRGAVEGSHRTASPNAGWPMGAAAGALGLRLEKAGHYVLNPVGKRPTS
                     SDVPVAQRLVGAGLAVAIVGTILVASRSAHHR"
     gene            complement(1009..1785)
                     /gene="cobS"
                     /locus_tag="VHS06_01085"
     CDS             complement(1009..1785)
                     /gene="cobS"
                     /locus_tag="VHS06_01085"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX2986759.1"
                     /translation="MLNSMVERIGRMAGAPLVAIGFLTVLPLPLPTALAMGPMGWPVA
                     AFPLAGAGIGALLALLNLALGPWVPGGLLVALLLAALMAITGALHLDGLMDTFDGLFG
                     GKAPEGRVAIMRDSRVGSFGVAAAITMLLVEYSALQSLPLQQRTGPLIAALCLSRWAM
                     AVTIWLFPPASNRGLAAGLKPAIRWWHVATATLLAVAITAWSLGVGSLAVLVAPVATL
                     LLLGWLALRRIGGITGDICGAVGELSEVSTLVVGTILAGL"
BASE COUNT          334 a          650 c          590 g          298 t
ORIGIN      
        1 ggtgagtctc ccctgacctg cccgggccag gggaggagcc gggcaggtca ggggagactc
       61 accctaccta tggtgcgcgg agcgacttgc caccagtatt gttcccacta tcgctacggc
      121 gagccctgct ccaacgaggc gctgagcgac aggaacgtcg ctgctcgtcg gacgcttacc
      181 cacaggattc aagacgtaat gtccagcctt ctccagccgc agccccagcg caccagccgc
      241 agcgcccatc ggccaaccgg cgttgggact tgccgttcgg tgggagccct cgacggcgcc
      301 tcgccacgca tcggcagggg atgtcccaac cggccgcgca gctatgaccg tggccagcgc
      361 cgtcaacctg gagggcagca ggttcagcag atcgtccagg cgggcggcaa ccttgccggt
      421 gtactcatac cggccacggt agccgatcat ggcgtccagc gtgttggcca tccggtagcc
      481 gtaggctccg ggcagcccaa aggcggcgta gagcaggagc ggggcgacca ccgaatcgct
      541 cgcgttctcc gctagcgtct ccaccgccgc tgcggccact tcggactcgt ccagggcgga
      601 tacgtcgcgg ctgaccagtt ggcccaccgc ctgcctggcg ccctccagat ccccctgttc
      661 gagcggtctc ctcactcggt ccgttgcgag aaagagatcc cgcagtgcga aggtcggttt
      721 gagcgcgacg gcctcggcga gagccccgac gagcgatcgt gccgggagaa gccgctggac
      781 gatcctggac gggacgatgg cggcggtcag acagattgac tccaccattc ccccatagat
      841 gaactgtcgg agcgcaccct ttccaggggc tcttgcttcg agtttaccca ccagacggcc
      901 cattgcagcg accgggtgcc atcggttggg aagttccccg aacagcaggt cgatccccag
      961 cgccagcacg attaccagtc cacgacgcat caacttcctc ttcgccccct acaaccccgc
     1021 caggatggtg ccgacgacca gggtggatac ctcggatagt tcgccgacgg cgccacagat
     1081 atccccggtg atgccgccga tccttcgtag cgccagccat ccgagcagta gcagggtggc
     1141 gacgggcgcg accaggactg ccagagagcc gacgcccaga ctccaagcag ttattgccac
     1201 tgccagcaac gtggccgttg ccacatgcca ccatcggatg gccggcttca atcccgccgc
     1261 cagccccctg ttgctcgccg ggggaaagag ccagatggtg acggccatgg cccagcgtga
     1321 gaggcagagt gccgcgatca gcggcccggt cctctgctga agcggcagcg actgcagcgc
     1381 cgaatactcc acgagtagca tggtgatggc ggcggccacg ccgaaactgc ccaccctgct
     1441 gtcgcgcatt attgccacgc ggccttccgg ggcttttccc ccgaatagcc cgtcgaaagt
     1501 gtccatcagt ccgtcgaggt ggagcgcccc tgtgatggcc atcagcgcgg ccagcaacag
     1561 cgcgaccagc agcccgcctg ggacccaggg gccgagcgcg aggttgagca gggccagcag
     1621 tgcaccgatg cctgcccccg ccagtgggaa ggctgccacg ggccagccca tgggccccat
     1681 cgcgagagcg gtcggcagcg gaagcggcag gacggtcagg aatccgatgg ccaccagggg
     1741 agcccctgcc atgcgcccta tcctctccac catggagttg agcatctatc ctccactaat
     1801 tcgggtcgat tgtgcgtcgt ggtctggtcg ccggcgacca ctttggctgt ggaaacctac
     1861 ctttatcgtc tt
//
LOCUS       DATALS010000057         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_5172,
            whole genome shotgun sequence.
ACCESSION   DATALS010000057 DATALS010000000
VERSION     DATALS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5172"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VHS06_01090"
     CDS             complement(<1..947)
                     /locus_tag="VHS06_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986760.1"
                     /translation="MRQFDEAALAAVVGDEDIGPAFARLCRMSVVRPMEHGLVLHDDV
                     RRAIADDLRWRRSDRYDGMKLRAVAYYKERLNSATPTEREWLLAELLFLWEDALTHAL
                     VYNDPEPGMVWIDAARQEDYSDITRIWNWWRPNVLDRAVGFEGELRGDSEFLEAMIRY
                     PGCRVRVARDRDGTVCGFSTAIPVCQQSIPILRIHSAFSPMIDTYWSGEELAALPVSY
                     EQSRTWFFVHLGYSETAVPEATQAALMRDHVGMYAQGDVYIIVAPHPDIKMLMQAWGY
                     ERVPGAKNWNWGPHQAVEGFVLDLRSIGVERWIESILSG"
     gene            complement(1009..1719)
                     /locus_tag="VHS06_01095"
     CDS             complement(1009..1719)
                     /locus_tag="VHS06_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2986761.1"
                     /translation="MLITSVIPSLTIGDAIDSLEESLFVGRKREIAAFRRWLDSDSEP
                     EVLLSVTGRGGIGKSTLLKAFARIARQSGRTVIHLDSRDFVHTKDGLARALGDSCDGD
                     IASYLNSRQPLVLLDTFDELGDLVRYFRDGLLPSLRTGVRVVVAGRHIVRTGRGDHWR
                     GNPLVRSLELGVFDGKECREYLVRRGLTDPDLIARVKRVTGGESTGGFPGCGHGAAVG
                     ASGVYSGARMASGCAIVG"
     gene            2162..3223
                     /gene="coxB"
                     /locus_tag="VHS06_01100"
     CDS             2162..3223
                     /gene="coxB"
                     /locus_tag="VHS06_01100"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX2986762.1"
                     /translation="MATNNGCVTPSAATRSSALLRRRLPLFLLVLPGLLAAGCDNTQS
                     ALNPKGPPAANIAMIWWIMFTISAAIFAVVVATLLYALHRRREPATETLERRPVDRKT
                     RTIGLVWVIASGAILLGMLAMDVRSNQFQSVAPSPVVFTLQVIGHDWWWEFNYLNSGV
                     TTANEAHIPVGRPVAISGESADVIHSFWVPELNGKLDLIPGHTNTMWMQADYPGVYRG
                     QCAEYCGDDHGLMALHIVADPAEQFAVWLNGQRQPAATPQDPAALVGMNGFPSSACAG
                     CHTIRGTKAGGNRAPDLTHVGSRRYIAAGTLENSPENLIRWISDPQGVKQGNFMPYLG
                     LDPNSEFVRSTAAYLWNLK"
     gene            3240..>4465
                     /locus_tag="VHS06_01105"
     CDS             3240..>4465
                     /locus_tag="VHS06_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019020682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2986763.1"
                     /translation="MATSSEAFERVWEDEPGILGWLSTVDHKRIGRRYIVTTFAFLLI
                     AGTEALALRTQLARPQLSILTAQSYNQLFTMHGTTMIFLFIMPIVAGFGNYLVPLMIG
                     ARDMAFPRLNAFSYWVLLLAGIFMYSSFFLGAAPNGGWFNYVPLTGSEWSAGPNIDFY
                     VLGLTFLGISTTAGAINFIVTIFRLRAPGMSIDRIPLFIWNILIMSFMIVFALPALTT
                     AGVLLFFDRNYGTHFYDPAAGGDPLLWQQLFWFFGHPDVYIFFMPPVGMASMIIPVFS
                     RRPIVGYAVIVAASISIGLISFGVWIHHMFATGLTLMEMSFFTAASVMIALPTAGQYW
                     AWLSMIWHGRPVFTTAFLFALAFLVEFIIGGVTGVMQSVTSFDWQVTDSYFIVAHFHY
                     TIIPSAVLPVMGGIYY"
BASE COUNT          863 a         1499 c         1227 g          876 t
ORIGIN      
        1 tcgccgctca ggatggactc gatccatcgc tccacgccga tactcctcaa gtccagaacg
       61 aagccctcaa cggcttggtg gggtccccag ttccagttct tggcgccggg cacgcgctcg
      121 tacccccagg cctgcatgag catcttgata tcgggatgcg gggcgacgat tatgtagacg
      181 tccccctggg catacatgcc aacgtgatcg cgcatcagag cggcctgagt cgcctccggc
      241 accgccgtct cactgtagcc gaggtgaacg aagaaccagg tcctgctctg ttcgtaactg
      301 actggcaggg cagccaactc ctccccgctc cagtatgtgt cgatcatcgg agagaatgcc
      361 gaatggatcc gcaatatcgg tatggactgc tggcatacgg gaatcgcggt gctaaaccca
      421 cacaccgtcc catcgcggtc cctggcgacc cgcaccctgc accccggata ccggatcatc
      481 gcctcaagaa actcactgtc accgcgcaat tccccttcga agccgaccgc gcgatccaaa
      541 acattgggtc tccaccagtt ccatatgcgg gtgatatcgg agtagtcctc ctgccgtgcg
      601 gcatcgatcc acaccatccc cggctccggg tcgttgtaga ctaacgcatg ggtgagggca
      661 tcctcccaga ggaacaacag ctcggccagc aaccattccc gctccgtcgg ggtggccgag
      721 ttcaagcgtt ccttatagta ggccaccgcc cgcaacttca tgccgtcgta ccggtccgac
      781 cgacgccagc gcaggtcatc cgcgattgcc ctccgcacgt cgtcgtggag aacgagaccg
      841 tgctccatcg ggcgaacgac agacatacgg cacaggcgag cgaacgccgg gccgatatct
      901 tcgtcaccta ccacggcagc caacgcggct tcatcgaact ggcgcataac ggaggccgcc
      961 tccagtagcc tcctgagcga tggatctgtc acatcattca gcaaccggtc aaccaacgat
     1021 cgcacaacca gatgccattc gggcgccgct gtaaactccc gaagccccaa ctgcagcacc
     1081 atgtccgcag ccagggaaac cgccagtgga ttcccccccc gtcacccgct tcacccgcgc
     1141 tatcaggtcg ggatccgtca gacctcgccg cacgaggtac tcccggcact cctttccgtc
     1201 gaacacaccc agctcaagtg atcgcaccag aggattgccc ctccaatggt cgccgcggcc
     1261 agttctcaca atgtgccggc ctgcaacaac cacccttacg ccggttcgca agctcggcag
     1321 caatccatct cggaagtagc ggacaagatc acccaactcg tcgaaggtgt ccagcaagac
     1381 tagaggctgt ctgctgttga gataggaggc gatgtccccg tcacaactat ctcccaaagc
     1441 ccttgccagt ccatccttgg tgtgaacgaa gtcgcgacta tcgagatgga tgactgtgcg
     1501 cccggattgt cgagcaatcc gggcaaaagc cttcaacaga gtgctcttgc cgatcccgcc
     1561 ccgaccggtg acgctcaaca acacttccgg ctcagaatcc gagtccagcc atcgccggaa
     1621 agcagcgatt tccctctttc ggccaacaaa gagggactcc tcgaggctat cgatagcgtc
     1681 gcctatggtg agcgagggta ttacactcgt gatgagcaag ggtcttacac tccagtgggc
     1741 ataagtgtca gctcctgccg gaatcttttc ggcgggcaac acatcgatga agtggactgg
     1801 aagcctggag gcaccatctt tctttcgtag tttgcagcag tccctgggga tcgtcatccg
     1861 agagaatacg catcttgaaa gtgacagtca accacgcacg cccgccgcga tgggtgagac
     1921 tactcagaga cgatacccgc ctcagcccaa ggtggttcct tagcatgctt cttgcaagga
     1981 atctggaaga aggattccac ggagcgtttc catgcacggc gatgggcgaa ctgccgcccc
     2041 atcgccttct gccctgccgt atccagctca caccgccaat atctcgcggg ccccgacaaa
     2101 ggcccgcttt caccacagac gtggggacaa tgtagcccgt cccggttgag gaaaacgagg
     2161 tttggcaacg aacaacggct gtgtaacccc ttcagcagct acccgatcct ccgcgctgct
     2221 ccgtcgccgg ctgcccctgt tcctgctagt tctcccgggg ctgttggccg ccggttgtga
     2281 caacacccaa tcggctctga accccaaggg ccccccggca gccaacatag ccatgatctg
     2341 gtggatcatg ttcaccattt ctgccgccat cttcgccgta gtcgtggcga cgctcctgta
     2401 tgcgctgcat cgtcgccgcg aaccggcaac cgagactctg gagcggcgcc ccgtcgaccg
     2461 gaaaacgcgc actatcggtc tcgtctgggt gatcgcctcg ggggccatcc tgttgggcat
     2521 gctggcgatg gacgttcgga gcaatcagtt ccagagcgtg gccccctccc cagtggtgtt
     2581 cacccttcag gttatcggcc acgactggtg gtgggagttc aactatctca actcgggagt
     2641 caccaccgcc aacgaggctc acattccggt gggcagaccg gtcgcgatca gcggcgaatc
     2701 ggccgacgtg atccacagct tctgggtgcc cgagttgaac ggcaagctcg acctgatccc
     2761 gggccacacc aacacgatgt ggatgcaagc cgactatccc ggtgtctatc gagggcagtg
     2821 cgccgagtac tgcggcgacg accacgggct catggccctc cacatcgtcg ccgatccggc
     2881 agagcagttt gccgtctggc ttaatgggca gcggcaaccc gccgcaaccc ctcaggaccc
     2941 ggccgccctg gtggggatga atggttttcc ctcctctgcc tgcgccggct gccacaccat
     3001 cagggggacg aaggccggcg gaaaccgggc gccggacctt actcatgtgg gaagcaggcg
     3061 ctacatcgcc gctgggaccc tggagaacag ccccgagaac ctgatccggt ggatatccga
     3121 cccgcagggt gtgaagcagg ggaacttcat gccctacctg gggctcgatc ccaacagcga
     3181 gttcgtccgc tcgaccgcgg cctatctctg gaacttgaaa tagccgttag gaggagccgg
     3241 tggcaacgtc ttcggaagcc ttcgaaaggg tgtgggagga tgagccgggc atcctgggct
     3301 ggctctccac cgtcgatcac aagcgaatcg gccgcaggta catagtcacc accttcgcct
     3361 ttctgctgat cgcgggcacc gaggcactgg cccttcgcac ccaactggcg aggccgcagc
     3421 tgagcatcct gaccgcgcag agctacaacc agctgttcac gatgcacggc accaccatga
     3481 tcttcctctt catcatgcca atcgtggccg gcttcggcaa ctacctggtg ccgctgatga
     3541 tcggggcccg agacatggcc ttcccgcggc tgaacgcctt cagctactgg gtgctgctgc
     3601 tggcgggtat cttcatgtac tccagcttct tcctcggtgc ggcacccaac ggtgggtggt
     3661 tcaactacgt gccactcacc ggctccgagt ggtcggccgg gcccaacatc gacttctacg
     3721 tcctggggct caccttcctc gggatatcca cgacggccgg cgcaatcaac ttcatcgtca
     3781 ccatcttcag gctgcgtgcg cccgggatgt ctatcgaccg catcccgctg ttcatttgga
     3841 acatcctgat catgtcattc atgatcgtct tcgcccttcc ggccctcacc accgccggcg
     3901 tccttctctt cttcgaccgc aactacggaa cacactttta cgaccccgct gccgggggcg
     3961 acccgctgct gtggcagcag ctgttctggt tcttcggcca ccccgacgta tacatcttct
     4021 tcatgccgcc tgtggggatg gcatccatga tcattccggt cttctcccga aggccgatag
     4081 taggctacgc agtcatcgtc gcggccagca tctccatcgg cctcatctct ttcggggtgt
     4141 ggatccacca tatgttcgcc acggggctaa cgctgatgga gatgagcttc ttcaccgcag
     4201 ccagcgtcat gatcgccctc cccaccgcgg ggcagtactg ggcgtggctg tcaatgatct
     4261 ggcacggcag accggtgttc accacggctt ttctctttgc gctggccttc ctcgtggagt
     4321 tcatcatcgg aggggtcacc ggtgtgatgc agtcagtaac cagcttcgac tggcaggtca
     4381 cggacagcta tttcatcgtc gcccacttcc actacaccat catcccctcc gcggtcctgc
     4441 cggtaatggg gggcatttac tactg
//
LOCUS       DATALS010000058         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_139516, whole genome shotgun sequence.
ACCESSION   DATALS010000058 DATALS010000000
VERSION     DATALS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139516"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VHS06_01110"
     CDS             <1..795
                     /locus_tag="VHS06_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2986764.1"
                     /translation="AMYPRRRRHLIRGDCKVEPTLHSVQVRGKPVPYQTMGEGDPVVL
                     VHGLAASSLWWLNNSPVLAKRHRVYLIDLPGFGALNRHRPLPLDLADCWLLEWMRAAG
                     IDRAHLVGHSMGGYICLRLAAHYPEMVDRLALVAPVGIPLQGSLLAHVIPLLIQAGPA
                     TPSLLPLMLYDVLRTRPTARWRTGLDILAEDARKYVGAISAPTLLIWGENDPVIPTSS
                     AELMRREIPDCRLMLMRGTSHVPMFERPEEFNDALLRFLNGEQVGE"
     gene            909..1925
                     /locus_tag="VHS06_01115"
     CDS             909..1925
                     /locus_tag="VHS06_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2986765.1"
                     /translation="MERRAVTIQDVASRAQVSIATVSRVLNDSGYPMRPDTKRRVLEA
                     IDALSFRPSSLARGLLGKPTRTIGLIIPDISNPYYPQLCRGVEDVAVENGYAVIFCNT
                     DRSQQKIQSYIDVLQEKQADGVIFTGGGVESGEELGPLEGLRDKVVVTGRHNGNYPSV
                     QVDNTRAAYDATFHLLSLRHRRIAFISGPHGLTSSDDRLRGFRQAMAKACIEVNEQFV
                     REGDFRFESGYLEAKAILSGPGERPTALFAANDRMALGAMSAAYDLGIRVPEDLSMVG
                     YDNTLTAQYVRPSLTTVSLPYYEMGATSARLLLRLLAGGTVQSTVWLPTQLVIRQSSG
                     DPIC"
     gene            1958..>2232
                     /locus_tag="VHS06_01120"
     CDS             1958..>2232
                     /locus_tag="VHS06_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX2986766.1"
                     /translation="MADLEKYNLLELPYSDVAEYLKAGNDVVMIPVGSCEKHGHHVPL
                     GVDSYTTMGTVELAAKKAKTLYSPLLPFGHSPHHMGEAGWGTGSISLP"
BASE COUNT          442 a          772 c          634 g          384 t
ORIGIN      
        1 gccatgtatc ctcggaggag gcggcattta atacgtggag actgcaaagt ggagccaacc
       61 ctgcacagtg tccaggtgag gggcaaaccg gtcccttacc agactatggg agaaggggac
      121 ccggtcgtgc tggtacacgg attggcagcc tccagcctct ggtggctgaa caacagcccc
      181 gtcctagcga agcggcaccg ggtatacctg atcgacctgc ccggcttcgg ggcgctcaac
      241 cgccatcgcc cactccccct cgatctcgcg gattgctggc tgctggagtg gatgagggcc
      301 gcggggatcg accgcgccca tctggtaggg cactccatgg gcggctacat ctgcctccgg
      361 ctggccgccc actatccgga aatggtggac cggctggccc ttgtcgcgcc ggtcggaatc
      421 cccctgcagg gctcgctgct ggcccacgtg atcccactgc taatccaagc aggacccgca
      481 acccccagtc tgcttcccct catgctgtac gacgtcctcc gaactcgacc caccgcccgc
      541 tggaggaccg gactggatat cctcgccgag gacgcgcgga agtatgtcgg agcgatcagc
      601 gcccccaccc tgctgatctg gggcgagaac gacccggtga tccccacctc ctcggcagaa
      661 ctgatgcgcc gggagatccc cgactgccgg ctgatgctga tgcgaggaac gagccacgtc
      721 ccgatgttcg agcgtcccga agagttcaac gacgcgctgc tgcgcttcct caacggggag
      781 caggtcggag aatagcgacg cgctccagat ctggcctttg gcgcgccaca tgcggaaaaa
      841 cccttgacac tgcccaggcg gctccataga atgacgctat agaaaacctt ttcagaaagc
      901 gtttatccat ggaacgaaga gccgtcacaa tccaagatgt cgcatcccgc gcccaggtct
      961 ccatcgccac cgtctcccgt gtcctcaacg actccggcta ccccatgcgc ccggacacca
     1021 agagaagagt cctggaagcc atcgatgcgc tctctttccg cccaagctcc ctggccaggg
     1081 ggttgctcgg caaacccaca cgcaccatcg gcctgatcat cccggacatc tccaacccct
     1141 actacccgca gctatgtagg ggcgtcgagg atgtggccgt ggagaacggc tacgccgtca
     1201 tcttctgcaa caccgaccgc agccagcaga agatccagag ctacatcgac gtcctccagg
     1261 agaaacaggc cgacggcgtc atcttcaccg gtggcggtgt cgaaagtgga gaagagttgg
     1321 gccccctcga ggggctgagg gacaaagtgg tggtcaccgg ccggcacaat ggcaactacc
     1381 cctccgttca ggtggacaac acccgcgcgg cctacgacgc taccttccac ctcctctcgc
     1441 ttcgacatcg ccgcatcgcc tttatcagcg gaccgcacgg cctcaccagt tcggacgaca
     1501 ggctcagagg cttccgtcag gccatggcca aagcctgcat cgaggtgaac gagcagttcg
     1561 tcagggaggg cgacttccgc ttcgaaagcg gctatctgga ggccaaggct atcctgtcag
     1621 gtccaggaga gcggcccact gccctcttcg cggccaacga ccgcatggct ctcggcgcca
     1681 tgtcggctgc ctacgacctc gggatacggg tcccagagga tctgtccatg gtcggctacg
     1741 acaacaccct taccgctcag tacgtccggc cttcgctcac caccgtgtcg ctgccctact
     1801 acgagatggg cgccacctcc gcccggctac tgctaagact gctagcgggc gggacggtcc
     1861 agagcacggt ttggctccct acccaactgg tcatcaggca gtcttccggc gaccccatct
     1921 gttagcgttt tgttaccaca caaagaggag gaaaacagtg gcagacctgg agaagtacaa
     1981 cctcttagag cttccctact ccgacgtggc cgagtacctc aaggcaggca acgacgtcgt
     2041 tatgatcccc gttggctcct gcgagaagca cggccatcac gtaccgctgg gcgtcgactc
     2101 ctacaccacc atgggcaccg tcgagttggc tgcaaagaag gccaagaccc tctacagccc
     2161 cctgctcccc ttcggccact ccccccacca catgggtgaa gccggctggg gcacgggctc
     2221 catctccctg cc
//
LOCUS       DATALS010000059         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_185172, whole genome shotgun sequence.
ACCESSION   DATALS010000059 DATALS010000000
VERSION     DATALS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185172"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VHS06_01125"
     CDS             <1..501
                     /locus_tag="VHS06_01125"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2986767.1"
                     /translation="GLVEFKTGQGPFVSAPTVDEVLELYDCRLMYEAHAIREGTERVD
                     DAFLHSMQQAIRQHELLFSESDGTYESRRKVLEADKDFHLLILSLWCNQKATAMYRQM
                     NVHIRSFQLAYIPDYRRETSIGEHHEIYAGLEKRDAETAAASVRHHIVASKEGFLKRV
                     RMAGLA"
     gene            complement(560..985)
                     /locus_tag="VHS06_01130"
     CDS             complement(560..985)
                     /locus_tag="VHS06_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2986768.1"
                     /translation="MQSSQSVDCVLIRPGGSYGGKQGLTYFAGVSAETAGSRALCMHM
                     LTIPPGGRAHAHLHEAHETAIFVISGEAEMWYGEGLSRHMDVKAGDFIYIPAGMPHLP
                     ANPSDTEPCIAVLARTDPNEQESVVLRPDLDELLRGMLE"
     gene            1260..1853
                     /locus_tag="VHS06_01135"
     CDS             1260..1853
                     /locus_tag="VHS06_01135"
                     /inference="COORDINATES: protein motif:HMM:NF022378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2680 domain-containing protein"
                     /protein_id="HEX2986769.1"
                     /translation="MKKLIIAAAAVAVLAVSTAVGVFAQTPPASQGQGWACPGWGTGR
                     QGNYDPATNPTIKALADKLGIQASDLVSQLQGGKSVADVAKDKGVAEQTLIDLLQAPM
                     VETMNVRVKYGYLTQDQAEQMKQNMADRIKTRLEQKGFFGGYGMMGDGTTGRGMMGNG
                     GMMGNGDGPRGGMMGGGMMGSGYGARGNGARAQGFTY"
BASE COUNT          458 a          733 c          765 g          397 t
ORIGIN      
        1 ggtctggtcg agttcaagac aggccagggt ccgttcgtat cggcgccgac agtcgacgaa
       61 gtgctggagc tgtacgactg ccgcttgatg tacgaggcgc atgccatccg cgaagggacc
      121 gagcgagtag atgacgcttt cctgcattcc atgcagcagg cgattcgaca gcacgagctg
      181 ttgttctccg aatcagacgg cacctacgag tcgcgccgga aggtcctgga ggccgacaag
      241 gattttcacc tgctcatcct ctcgctctgg tgcaatcaga aggccaccgc gatgtatcgg
      301 cagatgaacg ttcacatcag gtcatttcaa ctcgcctaca ttcccgacta ccggcgggag
      361 acgtccatag gcgagcatca cgagatctac gcggggctcg agaaacgcga cgcggagacg
      421 gccgccgctt cggttcgaca ccacatagtc gcatccaagg aaggcttcct gaagagggtc
      481 cgtatggcgg ggctggccta gcgcaacccc gccctccttc cgcaacgctc cctgcgcccg
      541 acactccccc ctatggattc tactccagca ttccgcgcag caactcatcc aggtccggac
      601 gcagcaccac gctctcctgc tcgttggggt cggtgcgagc cagcaccgcg atgcagggct
      661 cggtatccga ggggttggcc gggaggtggg gcatgcccgc cgggatgtag atgaaatccc
      721 cggctttcac atccatgtgc cggctcaatc cctccccgta ccacatctcc gcctcgccgc
      781 tgatgacgaa tatcgccgtc tcgtgggcct cgtgcaggtg ggcatgtgct cgaccgcccg
      841 gcgggatggt gagcatgtgc atgcagagag ccctggagcc tgcggtctcc gccgagacac
      901 ccgcgaagta tgtgagcccc tgcttgccgc cgtagcttcc acccgggcgg atcaggacgc
      961 aatccacgga ttgtgagctt tgcatatata tccctcctgc aactgggagc cgccccccac
     1021 agacggttcg aagcccccag gtcgtcgtcc acaagggttc ggtcggcgga taccgcggcg
     1081 atccggaacc gaatagttga gcagattccc tgccagtcgc ccgccgcgtg aatcccacag
     1141 attgtgcacg gcttcttcac tgcttcttca caaatgggcg atatcctttc aatcacacag
     1201 ggagggaaag cccccccgtg gaagacagat cgatggaaac ggctgaggag gcaaggaaca
     1261 tgaagaagct gatcatcgcg gcggcagcgg tggcggtgct ggctgtgagc accgcggtgg
     1321 gagtgttcgc tcagacccca ccggcatccc aggggcaggg atgggcctgc ccgggctggg
     1381 gcacaggtag gcagggcaac tacgacccgg ccaccaaccc aaccatcaag gcgctggcgg
     1441 acaagctggg catccaggcg agtgatctgg tgtcgcagct acagggtggc aagagcgtgg
     1501 cggacgtcgc caaggacaag ggagttgccg agcagacact gatcgatctg ctgcaggcac
     1561 ccatggtcga gacgatgaac gtccgcgtga agtacggcta cctgacccag gatcaggccg
     1621 agcagatgaa gcagaacatg gccgaccgga tcaagacgcg actggagcag aagggcttct
     1681 tcggcggcta cgggatgatg ggcgacggca cgacgggccg gggcatgatg ggcaacggcg
     1741 gaatgatggg caacggcgac ggcccgcgcg ggggcatgat gggtggcggc atgatgggct
     1801 cgggctacgg agctcgtggc aacggggcgc gggcgcaggg cttcacctac taggagcgca
     1861 gatcccccac tctcaagagg gccgggagca gacgaggcgt ctactcccgg ccctctcgtt
     1921 acatcggata gagcaccggc aggatggtgt tcacagcggg cagttgcggt agcaggccag
     1981 ggtggcgtac ggccacggtt cggctggcgc gagcgactgc cttccggctc gcagtcccca
     2041 acgcgcatgg agacgatgcg tcgaccagcc ccatctccat ggccttggcc ggttcacccc
     2101 cacgcgcgtg gggacaatcg gaagttgttg gcggcctcct ggcactcctt cggttcaccc
     2161 ccacgcgtgt ggggacaatg cttcggagca ctctgtcagt agccacccta gactgtacca
     2221 cccgtagccg cctggtgtag accactcgcc acccgacgtg ggttcaccct caggtaatgg
     2281 gaaggtagct gaggctcaca tggttggcgt tagcgcggac gccgacgtgg tcgccaccgg
     2341 acaggtattc agc
//
LOCUS       DATALS010000060         4157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_230396, whole genome shotgun sequence.
ACCESSION   DATALS010000060 DATALS010000000
VERSION     DATALS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230396"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..404
                     /gene="rplR"
                     /locus_tag="VHS06_01140"
     CDS             39..404
                     /gene="rplR"
                     /locus_tag="VHS06_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX2986770.1"
                     /translation="MIKPTSTRAARLKRHRRLRAKVEGSPSRPRLAVFRSLSHIYAQV
                     IDDTTGSTLVAASDMDPAIRGQVKGTKTEHAKAVGEAVATRALEHGINQVVFDRGGFL
                     YHGRVKALAEAARNAGLEF"
     gene            416..935
                     /gene="rpsE"
                     /locus_tag="VHS06_01145"
                     /pseudo
     CDS             416..935
                     /gene="rpsE"
                     /locus_tag="VHS06_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            928..1113
                     /gene="rpmD"
                     /locus_tag="VHS06_01150"
     CDS             928..1113
                     /gene="rpmD"
                     /locus_tag="VHS06_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX2986771.1"
                     /translation="MAKKLVITWTKSAIGYSQRQKDTIRSLGLRRLHQSVERPDSPEF
                     RGMVEKVRHLLRVEEEE"
     gene            1113..1564
                     /gene="rplO"
                     /locus_tag="VHS06_01155"
                     /pseudo
     CDS             1113..1564
                     /gene="rplO"
                     /locus_tag="VHS06_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            1561..2847
                     /gene="secY"
                     /locus_tag="VHS06_01160"
     CDS             1561..2847
                     /gene="secY"
                     /locus_tag="VHS06_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX2986772.1"
                     /translation="MIDSLVNAFRIPDLRRKLLYTFGMLVVFRFIAHVPVPGVNAEAL
                     GRLFESNQLIGMLDLFSGGAMKSFSVAAMGVYPYITASIIMQLLIPMVPRLQEMSKEG
                     ESGRNRINQYTHWLTVPLAFLQALGTGSLLANNQLGVISNFNLVTNFLPTLAILVSMT
                     AGTMLLIWVGELITENGIGNGISIIIFGGIVAGLPGMVAQSLFAGTNYLGLAIFMALG
                     LVTIAGIVVVQEAQRRIPVQFAKRIRGTRMYGGSSTHIPMRVNSAGMIPLIFAMSIML
                     FPGTVASYFIYAEGFGGDVARSINWLFNPANVVYWVLYFVMVVAFTFFYTMVIFQQQN
                     IPESLQKNGGFVPGIRPGRPTGEYLNKVLIRITWFGATFLGVVAILPFFVQNLTSVQS
                     LTLSSTALLIIVGVVLDTIKQLESQLLMRHYQGFIK"
     gene            2917..3573
                     /locus_tag="VHS06_01165"
     CDS             2917..3573
                     /locus_tag="VHS06_01165"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX2986773.1"
                     /translation="MFVVFLGAPGAGKGTQAGLVAERLGLVHLATGDILRDAVRRETQ
                     LGKQARGYMERGELVPDSLVVEVVVERLGQPDVAAGAVLDGFPRNIAQAVALDEALDR
                     RGEKVDVAVYLKVSETELIGRLAARWECGACRAPYHEVTKRPRTAGVCDLCGGALVQR
                     ADDKPETVRKRLEVYFEQTAPLLDYYRQKGVLAEVNGEQPIPEVSREVLEAIAVRAGR
                     "
     gene            3581..3820
                     /gene="infA"
                     /locus_tag="VHS06_01170"
     CDS             3581..3820
                     /gene="infA"
                     /locus_tag="VHS06_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX2986774.1"
                     /translation="MARAVRESGAGALEVEGVVTEALPNAMFRVELATGRRVLAHVSS
                     KIRLNFIRILPGDKVRLELSPYDLSRGRIVYRSTR"
     gene            3838..3951
                     /gene="rpmJ"
                     /locus_tag="VHS06_01175"
     CDS             3838..3951
                     /gene="rpmJ"
                     /locus_tag="VHS06_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX2986775.1"
                     /translation="MKVTASVKPRCEKCKVIRRRGVVMVICTNPRHKQRQG"
     gene            complement(4064..4157)
                     /gene="infA"
                     /locus_tag="VHS06_01180"
                     /pseudo
     CDS             complement(4064..4157)
                     /gene="infA"
                     /locus_tag="VHS06_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792406.1"
                     /note="stimulates the activities of the other two
                     initiation factors, IF-2 and IF-3; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-1"
BASE COUNT          840 a         1060 c         1425 g          832 t
ORIGIN      
        1 accggcggca agaagaagta gggccaaggg aggtagggtt gattaagcca acgagcacga
       61 gagcggccag gctcaagcgg catcgccggc tgagggccaa ggttgagggg agcccctcga
      121 ggccacgcct tgccgtcttc agaagcctca gccacatata tgcgcaagtg attgatgata
      181 ctactggctc gactctcgtg gccgcgtcgg atatggatcc ggccattcgg ggtcaggtaa
      241 aggggaccaa gaccgaacat gccaaggccg tcggcgaggc ggtagccacg cgcgccttgg
      301 agcacggcat caatcaggta gtgttcgatc ggggcggatt tctatatcac gggcgcgtta
      361 aggctctggc ggaggccgcc aggaacgccg gccttgagtt ctgagagagg cgggaatggc
      421 taagattgat ccatcggggc ttaccctcga agagagagtc gtaaaggtca accgggtcgc
      481 caaggttgta aagggcggcc ggcgcttcag cttcagtgcc gtggtcgtcg tgggcgacgg
      541 ccaggggatc gtgggtgcgg gtctgggcaa ggcgaatgag gtgcccgatg ctatccgaaa
      601 gggtgcagag gatgctaaga agaacctggt gcagatcccg ctgaaggggg aataccatca
      661 cccacgaggt gctggccagc ttcggcgccg cgaaggtgct gctgaagccc gcctcgccag
      721 gtaccggcgt tatcgccggc ggtggcgtgc gcgccgtggt tgaagctgct gggatcaggg
      781 acatactcac caagtcgctg ggcagcgcca acgcgatcaa cgtcgtgcag gccgcaatga
      841 cgggtctgag gatgctgaag gatcccgaga aggtagcgca gcagcggggc aagaagctga
      901 gcgacctggt agggaggagt gctaccagtg gctaagaagc tagtgatcac ctggactaag
      961 agtgccatag gctactcgca gcggcagaag gatacgatcc ggtcgctggg cctccggcgt
     1021 ctccaccaga gtgtcgaaag gccggactcc ccggaattcc gggggatggt ggagaaggtg
     1081 aggcaccttc tcagggtgga agaagaggaa taatgaaact tcatgaactg tcgccagccg
     1141 aaggcgctaa gaaggacaga aagagagtcg ggcgcggcaa cggctccggg cacggcacct
     1201 tttcggggcg tggttcgaag ggccagaagg ctcgatcggg cggcgcggtc aatccgaggt
     1261 ttgagggtgg ccagataccg atggtgttgc gcctgccgcg caagcggggc ttcaccaaca
     1321 tcttcaaggt ggagtaccac ctggtcaatc tagatggatt ggatcgcttc gaggccggca
     1381 gcgagataac gcctgagtct ctggcggcgg caggtgtcat tggcgacact gatttgccag
     1441 tgaagctgct gggcaggggg gggacatctc caagcagctc actatccgag tgcacgcctg
     1501 gtcacagagt gcacagcaga aagtcgaagc ggcggggggc gccctcgaaa ggctcaccgg
     1561 atgatcgatt cccttgtcaa tgcattcaga atacccgatc ttcgccgtaa gcttttgtac
     1621 accttcggca tgttggtggt ttttcgcttt atcgcccacg tgccggttcc gggtgtcaat
     1681 gccgaggcgc tgggcaggtt gttcgaaagc aatcagttga tcggtatgct ggacctgttc
     1741 tctggcggcg ccatgaagag tttctcggtg gccgccatgg gtgtttatcc ctacatcaca
     1801 gcatccatca tcatgcagct gttgatccct atggttccac ggctgcagga gatgtctaag
     1861 gaaggggagt cagggcgaaa caggatcaac cagtacactc actggctgac agttcctctg
     1921 gcattcctgc aggcgctggg tacgggctcg ttgctggcca acaaccagtt gggggttatc
     1981 tccaacttca acttggtgac caacttcctg ccgaccctgg cgatcctggt cagtatgacc
     2041 gccggcacca tgctgctgat ctgggtcggc gagttgatca ccgagaacgg catcggcaac
     2101 ggcatatcga tcattatctt tggtggcatc gtggccggtc tgcccggtat ggtagctcag
     2161 tcgctgtttg ccggcaccaa ctacctgggc ctcgcgatat tcatggccct cggactcgtg
     2221 actatcgctg gtatcgtggt ggtgcaggag gcgcagcggc ggatcccggt gcagttcgca
     2281 aagcggatca ggggcactcg gatgtacggc ggcagctcca cccacatccc gatgagggtg
     2341 aactccgcgg gaatgattcc gctgatcttc gccatgtcca tcatgctgtt ccctggcact
     2401 gtggctagct acttcatata cgccgagggg tttggcgggg atgtggctcg gtccatcaac
     2461 tggctgttta acccggccaa cgtggtgtac tgggtgctgt atttcgtgat ggtggtggcg
     2521 ttcaccttct tctacacgat ggtgattttc cagcagcaga acattccaga gagcctgcag
     2581 aagaacggcg ggttcgtgcc gggtatcagg cccggacggc cgacgggcga gtacctgaac
     2641 aaggtgctga tccggataac ctggttcggc gcgacgttcc tgggtgtggt ggctatcctg
     2701 ccattctttg tccagaacct gacgagcgtt caatcgctga cactcagcag cacggcgctg
     2761 ctgatcatcg taggtgtggt actggatacc atcaagcagt tggagtccca gttgctgatg
     2821 cgccattacc agggctttat caagtagtag ttgcccctag ccagacttgg tcggttggac
     2881 tgtgtacaac ttgatagagc ggttaggagg accgtggtgt tcgtggtgtt cttgggcgct
     2941 cccggcgccg gaaagggcac ccaggcgggc ctagtggcag agcggctggg actggttcac
     3001 ctggcgacgg gagacatcct gcgggatgcc gtgcggcgag agacgcagct cggcaagcag
     3061 gcgagaggct acatggagag aggggaactg gttcccgact ctctggtggt cgaagtggtg
     3121 gtagagcggc tgggccaacc ggatgtggca gcgggtgcgg ttctggacgg ttttccaagg
     3181 aacatcgccc aggccgtagc gctggatgag gcgctggacc gtcgcggcga aaaggtggat
     3241 gtggccgtct atctgaaggt ttcggaaaca gagttgatcg gaaggctggc ggcccgatgg
     3301 gagtgtggag cctgcagggc cccctaccac gaggtgacta agcgtcccag gacggctggt
     3361 gtgtgtgacc tgtgtggtgg cgcgctggtt cagcgggcgg acgacaagcc ggagaccgtg
     3421 aggaagaggc ttgaagtcta cttcgagcag actgctcctc tgctggacta ctatcgacag
     3481 aagggcgtac tggccgaggt caatggcgag cagccgatcc ctgaggtgag ccgtgaggtg
     3541 ctggaggcga tagccgtcag ggcgggccgg taggagaggg ttggcgagag cggtgaggga
     3601 gtccggtgct ggggctctgg aggtcgaggg ggtagtcacc gaggctttgc ccaatgccat
     3661 gttcagggtt gagctggcaa cggggcgcag ggttttggcg catgtctcta gtaagattcg
     3721 tctgaacttt atcaggatct tgcccggtga caaggtgagg ttggagttat ctccttacga
     3781 cctttctcgc ggccggatag tgtatcgatc gactagataa acgctcggag tgatccaatg
     3841 aaggtaacag cgtcggttaa gccgcgttgc gaaaagtgca aggtaatccg gcggcgcggg
     3901 gtcgtcatgg tgatttgtac gaatccccgg cacaagcagc gccagggatg aagggatagg
     3961 gagacggggt gaatgggaga cggagccgag ctgttgtctc cctggctccc cgtcaccccg
     4021 tctcccgatc gaacaagagg ttaatgcgag ttcttgtgat atagggaaag atcctgataa
     4081 agttcagacg aatcttacta gagacatgcg ccaaaaccct gcgccccgtt gccagctcaa
     4141 ccctgaacat ggcattg
//
LOCUS       DATALS010000061         5283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_5381,
            whole genome shotgun sequence.
ACCESSION   DATALS010000061 DATALS010000000
VERSION     DATALS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5381"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VHS06_01185"
     CDS             complement(<1..657)
                     /locus_tag="VHS06_01185"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX2986776.1"
                     /translation="MEKRVKAVNEMLPFLDRMIASMTEAVLVIDVSGLVVAVNQAAID
                     LMDLPDRTSALRPLSEYNQLIKGWHVAGEVFPPDELLRFTKGTAITRQLATITTSAGV
                     EHIAEFTVTPIRDDKGQIYLGMMIASDITREHRLRAYWQAVGTVAQGLSTELVVDRVL
                     EAVLDQVVETLGGEVVIGVWGIDEGTGKLSLMTYRGLSKPIVDMLRTIPLDKATSIVE
                     A"
     gene            complement(805..1785)
                     /locus_tag="VHS06_01190"
     CDS             complement(805..1785)
                     /locus_tag="VHS06_01190"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2986777.1"
                     /translation="MHTDNGKIVLSRNGIRDMTDGQGKVIITGVSGMVGFHLAERLCR
                     LHPHTEVVGICRHDGRNVEDLLEHPNFRFVAADILEPSCYESELVGTDTLYHLAAQSA
                     VFKGRLDPLSDLRANVQGTMQLALCAATAGVRKVVFTSGCAVYVSQDRVSEDQVGQPP
                     SVYGASKLAAENYLRVIGDTRGFRYTILRLSRVYGPRMTRGALWDLAQDLKAGREGDI
                     FAHPDSVFDFIYVRDVVDALVLAAGQEWDGVTANISSAEGTWLRDLHARFEVMTGRTI
                     PLKKREGTPVVETVLNDRARALGWVPSYSLHEGMAETLAWLLPDLCRQGY"
     gene            1940..3211
                     /locus_tag="VHS06_01195"
     CDS             1940..3211
                     /locus_tag="VHS06_01195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2986778.1"
                     /translation="MLGHSAGLDGVRREYVDRGGRTIRLLAAASVTVWSIVLSFYLLT
                     YRDALPEVMFGNDFVAFWTGASMVRHGEGSRLYDLDAQRSFQIRLRQEQAATEGMREA
                     TGASPYWNPPPLALLVIPFTVMPAFWGYVAWTAAGLLFTVLAVALPLRRHPYRLPVVI
                     VLLGFGAFAATLTQGQVNWIFLLSLSLGMLALGEGRPYLGGMLLGLLWLKPQYASLFP
                     LVFLMKGRWRELTGMVGMGALLGAISLAIIGVDGVETYLRSMATVSGFHESPGTAVCP
                     IEMVNWRALLLSFWPQIPEDAGRQLVTALGLITALLSLLVWSGPWDAASPRFPRQVLA
                     LLIATIVASPHSHFHGMLLLLAPVAFMAARSVGEAPYRRAWIAFLAGGYGLFFTFPPF
                     RAPFALLLAMFAIAIVTIQSRVEGEVAPFAG"
     gene            3192..4454
                     /locus_tag="VHS06_01200"
     CDS             3192..4454
                     /locus_tag="VHS06_01200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2986779.1"
                     /translation="MRLSPVELQTRPAAPSTWANGRLIALLVAVWLGESVFALSFVVR
                     SWPDVVFSLDFTSFWSGASLLRDHVGSRLYDLGVQQAFQVALRHEQAVDSQIRELSGF
                     MPYWNPPPLLLLFLPVSLLPTAWGYFAWLAVSVAAFALSVSVPLKGVSQGRSKAVVLL
                     TYMGVAGTLLEGQVNGILLLAFSLGLLALVSGRCFLGGLLLGTLWLKPQYALLLPLLF
                     VFKGRWRETAGMISAGLLIGLTSLAMIGSDGVVRYLAALGQVGGYYAAPGSFASPESM
                     VNWRALLQNLLPSLPEEAGSLMVWSLGIATALLSLLAWAGPWDAQSPQFPLRMLVTVA
                     AILVASSHSHLHGTVLLLAPLTLVLARCEKLPVSSRLWHSLLVSGYVLALVAWVFRGF
                     SWLFVPYLVTFAAVLVFGLRTSDPEVGQ"
     gene            4451..>5283
                     /locus_tag="VHS06_01205"
     CDS             4451..>5283
                     /locus_tag="VHS06_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986780.1"
                     /translation="MSDVATVHRAGVPEREVAYTIGSLEMDPGHRKGWAPTLERRALA
                     VWSSALGAGLAAGIAYLAIAVAFGVDHLGQGNGPHFLYLADAFLHGRLSLLALPGEPT
                     DLLLYQGQYFVYYPPLPALLLMPLVALFGPSMSDAAITIGVGALNVGLVSLLLSSLER
                     SGLISLDAVRRAWVTAFFALGTTHSTVAPHPHAASMALLMGFTAMCAAYVAAVRLSGA
                     KGALVAGSLVGAAFLCRNTLILPGLWVARAKKAVTQARLTASREIRPDRSSDDSSRDT
                     RP"
BASE COUNT          820 a         1618 c         1626 g         1219 t
ORIGIN      
        1 ggcttcgact atggaggttg ccttatccag ggggatggtt ctcagcatgt caacgatggg
       61 cttggacagc ccccggtagg tcatgaggct cagtttacct gtcccctcat cgatgcccca
      121 gaccccgatc actacctcac cgcccagagt ctcgaccacc tggtccagta ccgcctccag
      181 cacccggtcc accaccagtt cggtagacag cccttgagcg acggtcccca cggcctgcca
      241 gtaagccctt agtctgtgct ccctcgtgat gtccgaggcg atcatcatgc ccaagtagat
      301 ctgaccctta tcgtctcgga tgggggtgac cgtaaactcg gctatgtgct ccacccctgc
      361 cgaggtggtg atggtcgcca actggcgggt aatggccgtg cccttcgtga agcgaagaag
      421 ttcatcaggg gggaacacct ccccagcgac gtgccatccc ttgatcagtt ggttgtactc
      481 gctgagagga cgaagggcac tagtcctgtc ggggaggtcc atgagatcga tggcggcctg
      541 gttgacagcc actaccaggc ccgagacatc gatgacgaga actgcctcgg tcatcgatgc
      601 gatcatccgg tccaggaagg gaagcatctc gttgactgcc ttgacccgct tctccatcgg
      661 tcaattctcc tgaagccgat gtccgcagag actctgtcac cgtctggtct cgcaggcagt
      721 gtaccataag gatgctcaac ttacgccggt ccggaccaag ccgcttgcac gtagagcctc
      781 ggtgtttagc gacgaatggt cgagttagta accctggcga cacaggtccg gcaacagcca
      841 ggccagggtc tctgccatcc cttcatggag agagtagctc ggcacccacc ccagtgcccg
      901 cgcacgatcg ttgagcacgg tctcgaccac gggtgttccc tctcgtttct taagtgggat
      961 ggtgcgcccc gtcatcacct cgaaccgtgc atggaggtcg cgcagccacg tcccctccgc
     1021 gctgctgatg tttgccgtca cgccgtccca ctcctgccct gccgcaagca cgagtgcgtc
     1081 caccacatct cggacataga tgaagtcgaa gaccgagtcg ggatgtgcga agatgtcgcc
     1141 ctctctgccc gccttgaggt cctgcgccag gtcccacagc gcgccgcgag tcatgcgtgg
     1201 gccgtacacc ctcgagagtc tcaggatggt gtatctgaag ccccgagtat cgccgatcac
     1261 ccgcagatag ttctccgccg cgagcttgct ggcgccgtac accgacggtg gctgccccac
     1321 ctgatcctcg ctcacgcgat cctggctgac gtagaccgcg cagccggatg tgaacacgac
     1381 cttccgcacc ccggccgttg ctgcacacag ggcaagttgc atggtgccct ggacgttggc
     1441 ccggaggtca ctcagcgggt ccagtcgccc cttgaagacg gccgactggg ccgcaaggtg
     1501 gtacagcgtg tctgtgccca ccagttcgct ctcgtagcag gatggctcca gtatgtcggc
     1561 ggcgacgaag cggaagttgg gatgttccag gagatcctct acgttccgtc catcatgccg
     1621 gcagatgccc accacctccg tatgggggtg caaccggcac agccgctccg cgaggtggaa
     1681 ccccaccatc ccgctgacgc ccgtgatgat gactttgccc tgcccgtcgg tcatgtctcg
     1741 aatgccgttt cttgatagga cgatcttccc attgtcagtg tgcaactacc tggtagcctt
     1801 atgctcagcg cagaaggctc ttgtgggaga tgtgcgtcct gtgaggtatg agactacatt
     1861 cccatcccgt tgccgtcctt cggatccacc gctacatgat acagctttag cctctagaga
     1921 gtgtgcagaa cacgcttctt tgctggggca ctcagccggt ctggacggcg tgcggcggga
     1981 gtatgtggac cgcggtggga ggacaattcg acttctggcc gccgcttcgg tgaccgtatg
     2041 gtcgatcgtt ctgtctttct accttctcac ctaccgtgat gcgcttccgg aagtgatgtt
     2101 cggcaacgac ttcgtggcct tctggaccgg tgcgtcgatg gtgcggcatg gagaaggctc
     2161 caggctgtac gacctcgatg cgcagcggtc gtttcagatc cggttacggc aggagcaggc
     2221 cgccaccgaa gggatgcgag aggctactgg agcgagtccc tactggaatc ctccgcccct
     2281 tgcgttgctg gtcatcccct tcaccgtcat gccggcattc tggggctacg tggcctggac
     2341 ggccgccgga ctgctcttca cagtccttgc agtcgctctt ccgctgaggc ggcatcccta
     2401 ccgcctcccc gtcgttatcg tgctgcttgg tttcggcgcg ttcgcggcta ccctgactca
     2461 gggacaggtg aactggatct tcctgctttc tttgagcctc ggcatgctgg cgctggggga
     2521 gggtaggccg tatctcggtg ggatgttgct gggactgttg tggctgaagc cgcagtacgc
     2581 atctctcttc ccgctcgtgt ttctgatgaa gggcaggtgg cgagaactga ccggcatggt
     2641 ggggatgggt gcgcttttgg gggcgatctc gctggccatc atcggggtgg acggagtgga
     2701 gacgtatcta cgctccatgg cgacggtgag tggctttcac gagtcgcccg gcacggccgt
     2761 ctgcccgatc gagatggtga actggcgtgc tcttctcctg tctttttggc ctcagatccc
     2821 ggaggacgcc ggcaggcagt tggtcacggc gcttggtctg attacggcgc tcctgtctct
     2881 cctggtgtgg agcgggccgt gggatgcggc ttcgccccgc tttccgagac aggttctcgc
     2941 gctgctgatt gcgacgatcg tcgccagtcc ccacagccac tttcacggaa tgctgttgct
     3001 gctggcgccc gtcgctttta tggctgccag atcggtgggc gaagcgcctt accgacgcgc
     3061 atggattgcc tttcttgcgg ggggctacgg gctgttcttc acctttccac cgttccgtgc
     3121 cccatttgca ctcctactcg ccatgttcgc gattgccata gtgaccattc aatcgagggt
     3181 ggagggagaa gttgcgcctt tcgccggttg aactccaaac cagacccgca gcgccgtcaa
     3241 cctgggcaaa cgggcgcttg atcgcgctgc tggttgccgt atggcttgga gaatccgtct
     3301 ttgccctctc cttcgtggtt cgatcgtggc cggacgtcgt cttttcgctg gatttcacct
     3361 ccttctggtc cggggcctct ttgctgagag accatgttgg gtcccgactg tacgaccttg
     3421 gcgtacagca ggccttccag gtggcccttc gacacgaaca ggccgtcgac tctcagatac
     3481 gcgagttgtc gggtttcatg ccgtattgga atccgccgcc attgcttcta ctctttctgc
     3541 ccgttagcct gttgcccact gcgtggggct atttcgcgtg gcttgctgtg agcgtcgcgg
     3601 ctttcgccct gtccgtttcg gttccattga agggtgtgtc ccaaggacga tccaaagccg
     3661 tggtgttgct gacatacatg ggggtggctg gaacgctgtt ggagggtcag gtcaacggca
     3721 ttctacttct ggccttcagc ctgggcctgc tggcgttggt ttccggccgt tgcttcttgg
     3781 gcggactgct gctgggaacg ctctggttga agccgcagta cgcgctgctt cttccgctgc
     3841 tgttcgtatt taagggacgg tggcgcgaga ctgcaggcat gatctctgcc ggtctgctca
     3901 tcggactcac atcgctggcc atgatcggtt cggacggggt ggttcggtac ctcgctgctc
     3961 ttggacaggt gggaggatac tacgctgctc ccggttcgtt tgcgagcccg gagagtatgg
     4021 tcaactggcg agcactgctg cagaatctgc ttccgagcct ccccgaggag gcaggatcgc
     4081 tgatggtgtg gagtctcggg atcgctaccg cgttgctctc cttgctggcc tgggcggggc
     4141 cctgggatgc acagtccccg cagtttccct tgcggatgct ggtcactgtg gcggcgatcc
     4201 tggttgccag ttctcacagc caccttcacg gaacagtgct gctgctcgcg cccctgacgc
     4261 tcgtcctggc gaggtgcgag aagctacccg tctcatccag gttatggcac tccctgctgg
     4321 tgtctggata tgtgctggcc cttgtcgcct gggtgttcag ggggttctcc tggttgtttg
     4381 tcccttacct agttaccttt gccgcggtat tggtgttcgg cctccgcact tccgatccgg
     4441 aggttgggca gtgagcgatg tggccacggt gcaccgagcc ggggtgcccg agcgggaggt
     4501 cgcgtacacc ataggtagtc tggagatgga tccggggcac aggaaggggt gggcgccgac
     4561 actggagcgt cgagcccttg ctgtgtggag ttcggctctt ggggcaggtc ttgcggcggg
     4621 gattgcctat ctagccatcg ccgtggcctt cggtgtcgac cacctcggcc agggtaatgg
     4681 gcctcacttc ctatacctgg ccgacgcctt cctccacgga cggctgtcgc tgttggcatt
     4741 accaggggag ccaacggacc tgctgctgta tcagggccag tacttcgtct actatccgcc
     4801 cctccctgct ctactgctca tgccgctggt cgccctattc gggcccagca tgagtgatgc
     4861 cgccatcacc atcggcgtgg gagccctgaa cgttggcctc gtgtctctac tgctgtcatc
     4921 actagagcga tcaggcctga tttctctgga tgcagtgagg cgagcctggg ttacagcctt
     4981 cttcgctctg ggcacgaccc actccacggt agcaccccat cctcatgctg cgagcatggc
     5041 gttgctgatg ggattcacgg cgatgtgcgc tgcctatgta gctgccgtcc ggctttccgg
     5101 tgccaagggg gctcttgtgg ccggatctct tgtgggtgca gccttcctct gcaggaacac
     5161 cctaatcctg ccgggcttgt gggtggccag agcgaagaag gctgtaaccc aggctcgcct
     5221 cactgcatcc agagaaatca ggcctgatcg ctctagtgat gacagcagta gagacacgag
     5281 gcc
//
LOCUS       DATALS010000062         6141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_230482, whole genome shotgun sequence.
ACCESSION   DATALS010000062 DATALS010000000
VERSION     DATALS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230482"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VHS06_01210"
     CDS             complement(<1..323)
                     /locus_tag="VHS06_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2986781.1"
                     /translation="MEKRAGGPSGQSTQIAQFASRYRWVILPAIVLAQISISLAPQGV
                     APLTPFLKSDLHLSLAQVGLVGSAISIGTGLFLIVAGWLVDRVGVRYVLLGGQLVVGS
                     ALAATA"
     gene            complement(431..1396)
                     /gene="meaB"
                     /locus_tag="VHS06_01215"
     CDS             complement(431..1396)
                     /gene="meaB"
                     /locus_tag="VHS06_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX2986782.1"
                     /translation="MELVQRMLAGDQVALAKLITMVENHSPEVPQIMRLIHSHTGKAH
                     RIGITGPPGAGKSTVTDKLTALLRGKGATVGIVAIDPTSPFSGGALLGDRIRMQQHYL
                     DPGVFIRSMGTRGSHGGLSISTKDVVKLLDAFGKDYVIVETVGVGQTELDVVSATDTT
                     IVILVPESGDAVQTMKAGLMEIADIFVVNKADRGGADRVKTQLEIMLHMSPPREFDWE
                     VPVLLTEAEFGKGVEELYDAIDRHRTALQANDELSRRRQEHSKIELIEVVEDLVRARL
                     VDKSTADGLFADFVRKVQRGELDPYTAAHEIFASRQLLRELFVNK"
     gene            1901..2386
                     /locus_tag="VHS06_01220"
     CDS             1901..2386
                     /locus_tag="VHS06_01220"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX2986783.1"
                     /translation="MQVLPGKLKAEELIPSFTLPSAGGSMIGPRMYHHRRSLVLFFFH
                     DGRCQVCERLLTDFAGRYDEIRSENGEVLAVTTASAGDARAMANRLNLPFPLLYDTSG
                     KVVERFTQVEQDSGRVVPSVVITDRFGAVYTTLLVHETKPPSADAVLEWLNFIELQCP
                     E"
     gene            complement(2655..2954)
                     /locus_tag="VHS06_01225"
     CDS             complement(2655..2954)
                     /locus_tag="VHS06_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HEX2986784.1"
                     /translation="MKFLVMWEFAGLGLRPEIVQAVMRMPDYAKKISDQGKLIARYHI
                     VGKHGGAWIYDVASNEELERLLAVAPVYNYVRYDIYPLADMETPADILRPGEEPS"
     gene            3259..4902
                     /locus_tag="VHS06_01230"
     CDS             3259..4902
                     /locus_tag="VHS06_01230"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX2986785.1"
                     /translation="MAKYVFVTGGVVSSVGKGVTVASIGRLLKSRGLSAAAMKLDPYL
                     NVDPGTMNPYQHGECFVTDDGAETDLDLGHYERFIDTNLTRDSNVTTGQVYSSVLSRE
                     RQGDFLGGTIQVIPHITNEIKRRIKRVANDTGADVVIVEVGGVVGDIESLPFLETLRQ
                     LHRELGQDNALYIHVTLLPHLSSTGELKTKPTQHSVQELRRIGIQPDVIVCRSELPVS
                     DDLKDKIALFCDVERGAVIPLLTVDTIYEVPLILEASHLGRLLTSRLKLKAQAPDLSQ
                     WVELVEKIKMPKKELWVGLVGKYVELPDAYLSVREALKHAAIFHNRDLRVRYVDSEML
                     EQEGGEELLRGLDGIVVPGGFGQRGIEGKIRAARYARENGVPYLGLCLGLHVMVVELA
                     RHALGSNDAHSTECDPKTMHPVIHLLPEQRSVRAKGGTMRLGAYPCQMVLGTKAAAAY
                     GEGEISERHRHRFEFNNDYRGMLQEAGLVISGLSPDGKLVEIVELRDHPWMVGTQAHP
                     EFKSRPTRPHPLFRDFVAAAKQTLHEGDQRPLPLDQMLA"
     gene            4938..5402
                     /locus_tag="VHS06_01235"
                     /pseudo
     CDS             4938..5402
                     /locus_tag="VHS06_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
     gene            complement(5825..>6141)
                     /locus_tag="VHS06_01240"
     CDS             complement(5825..>6141)
                     /locus_tag="VHS06_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX2986786.1"
                     /translation="NQALLAAAAGATYVSPFVGRLDDIGQDGMQMVAETVAIYRNYGI
                     TTEVIAASIRHTMHVVAAARVGAHIATVPFKVLMQMVDHPLTSAGIDRFLKDWASVNK
                     AR"
BASE COUNT         1259 a         1720 c         1912 g         1250 t
ORIGIN      
        1 gcggtggctg ccagggcact gcccacgacg agttgccccc cgagcaaaac gtatcgaacg
       61 ccaacgcgat cgaccagcca ccctgccacg atcaggaaca gtccggtgcc aatgctaatg
      121 gcggagccga cgaggccaac ctgagccaag ctaaggtgga gatcgctctt caggaacggc
      181 gtcaaaggcg cgacgccctg aggagccagg gagatgctga tctgggcaag gacgatagca
      241 ggaaggatca cccatcgata gcgagaggcg aactgggcta tctgagtcga ttgccctgat
      301 ggtccgcccg ctcgtttctc caaaggcccg ctcggtgagg cgggcatatc ctcaggctcg
      361 tagtgcaaaa cgtgtactct ttctgctcgt cgacagtgta ggtcggctgt tcgagagcgc
      421 catctctccg ctatttgttc acaaagagct cgcgcagcag ttgtctgctg gcaaatattt
      481 cgtgggcggc ggtgtacgga tcgagctcac cgcgctgcac cttccgcacg aagtcggcga
      541 acagaccatc ggcggtcgat ttgtccacca gacgggcgcg aaccaggtct tcgacgactt
      601 cgatcaactc gatcttgctg tgttcttgcc tgcggcggga cagctcgtcg ttggcctgaa
      661 gagccgtgcg atggcggtcg atggcatcgt aaagctcctc gacgcctttg ccaaactcag
      721 cttcggtgag tagcaccggc acttcccagt cgaactcccg cggtggactc atgtggagca
      781 tgatctcgag ctgggtcttg actctgtcgg cgccgccgcg gtcggccttg ttgacgacga
      841 agatatccgc gatttccatc aatcccgctt tcatcgtctg cacagcgtca cccgactcag
      901 gcacgaggat gacgatagtg gtgtcggtgg cgctgacgac gtcgagctcc gtctggccaa
      961 cgccgactgt ctcgacgatc acgtagtcct taccgaaggc gtccaacagc ttgacgacat
     1021 ccttggtcga gatcgagagg ccaccgtggc tacctcgggt gcccatgctg cgaatgaaca
     1081 cgccggggtc caggtagtgt tgctgcattc tgatgcggtc gccgagtaag gcgccgccag
     1141 agaagggact ggttgggtcg atggccacga taccgaccgt ggcccccttg cccctcagga
     1201 gcgcggtgag cttgtcggtc accgtgctct taccagctcc gggaggtccg gtgatgccga
     1261 tgcggtgggc tttgcctgta tgagagtgaa tgaggcgcat gatctggggt acttctggcg
     1321 agtggttctc caccatagtg atgagcttgg ccagggctac ctggtcgccg gcgagcattc
     1381 tttgcaccag ttccatgtct tgttcaccaa acctctctat cctgcgctcg ttctacgccg
     1441 aacgtctagt cgtgcgacgc ttcagggttg gcgaaggccg ggctgtatcc ctgtcgtctc
     1501 gcttccgcgt cggcgcatga tgatgttctc tcgattggcg tgcctcggtc cgcggctatt
     1561 ataacctagt cggcaaaaaa tatctccagg agatcgcttg ttctgacccc atccgacgga
     1621 tgccaattgc cgttggagtg gtcctggtgc ggccctgttc ctctctcgaa acagcatagg
     1681 gcggggccct gctggcgtga cgcgcacgac cgagcctcca tttgattctt gtctctgtga
     1741 aggcagaagc aggaggaaga agagggctat cggcagagag ggccgcggtt cgccgaccga
     1801 gtgttccacc ttcacgccct ggtgcccagt gtcgtgcatg cgccggtggc ctgggaattg
     1861 ctgccatgtg tgaagatgct ttggctgaca ggaggagtgt atgcaggtgt tgccgggaaa
     1921 actgaaggct gaggagctca tcccaagctt cacgctaccg tcggcgggtg gtagtatgat
     1981 cgggccgcgc atgtaccatc accggcgcag cctggtgttg ttcttttttc acgatggacg
     2041 gtgccaggtg tgcgagcgac tgctgaccga tttcgctggg cggtatgacg agatcaggag
     2101 tgagaacggc gaggtgctgg cggtgacgac ggcatccgcg ggcgacgccc gggcaatggc
     2161 caacaggctg aacctaccct tcccgctact ctacgacacg tcgggcaagg ttgtggaacg
     2221 ttttacgcag gtcgaacagg atagtggtcg tgtggtgccg agcgtggtga tcaccgaccg
     2281 cttcggggcc gtctacacga cgctcctggt gcacgagaca aagccgccga gcgccgatgc
     2341 ggtgctagag tggctgaact ttatcgagct gcagtgtcct gagtgaggcg cgccagagtg
     2401 gccgatgccg taggcatcgg gaggtaagca gatcacccag gtggggtgcc actgacggag
     2461 gacgtcaaga acaaacccag ggtgtgcttc gcggtggacg aagtggagaa ggtgcgtctg
     2521 ggcgaccgcg cgtgtgacca caacattcgc cgcgggaggg tcatcgcttt tggccaagcg
     2581 cccatcgtct cagacaccgc gatgaagcgg cgcacttctg gtcacagttg cccccacatt
     2641 catgtcttct tgtctcagga cggctcttct cccggcctca agatgtcagc aggtgtttcc
     2701 atatctgcca gaggataaat gtcgtaccgg acataattgt agaccggtgc cacggctaac
     2761 aggcgctcca actcctcatt ggaggcaacg tcatagatcc aagcgccacc gtgtttgccg
     2821 acgatgtggt agcgggcaat cagctttccc tgatcgctta tcttcttggc atagtccggc
     2881 atacgcatca ccgcctgaac gatctctgga cgaagcccca ggccagcaaa ctcccacatc
     2941 acaagaaact tcatttggca agtcctcccg atgcagtcaa ctctactgct aatgatacca
     3001 cgggttggtg gttcttgcag cgtatcgaga tataccgcgg agccgaattc ctcgcccagt
     3061 cggagagact tcgtcctttc ggtccggcct catcaggtag gggcatctgc tattaagtaa
     3121 tcataccaat tactggcttt caggcagata ggcgtgcaaa acacccgccg gatttgctag
     3181 aataggcggt gttgaagacg tttacggtgc ggggtgggca tcctttggcg gggtcggcat
     3241 cctgagtgag ggacggatat ggctaagtac gtgtttgtga ctggcggagt tgtttcctcg
     3301 gtggggaaag gagttaccgt cgcttcaatc ggtcgactgc tcaagagccg tggactgtcg
     3361 gcagcggcga tgaagctgga cccgtatctg aacgtggatc ctgggacaat gaacccctac
     3421 caacacggag agtgcttcgt caccgacgac ggtgcggaga cagacctgga cctcggtcac
     3481 tacgaacgct tcatcgacac aaacctcact cgcgactcca acgttaccac gggacaagtc
     3541 tattcctcgg tgttatccag agaacggcaa ggcgacttcc taggcggcac gattcaggtt
     3601 attccccaca ttacaaacga gatcaaacga cgcatcaagc gtgtagccaa cgatacaggg
     3661 gcggacgtgg tcattgtcga ggtgggtgga gtagtcggcg acatcgagag cctgccgttt
     3721 ctggaaacgc tgaggcaact gcaccgtgag ttgggccagg acaacgcgct gtatatccat
     3781 gtgacgctgc tgccacatct gagctcgact ggcgaactga agacgaagcc cacgcaacac
     3841 agcgttcagg agttgcgccg cattggtatt caacccgacg tcattgtctg tcgctcggaa
     3901 ctgccggtaa gcgatgatct gaaggacaag atagcgctgt tctgcgacgt ggagcgaggg
     3961 gcggtcatcc cattgctgac cgtcgatacc atctatgagg tgccactcat cctggaggcg
     4021 tcccatctcg gcaggctgtt gacaagtcgg ctcaagctga aggcgcaagc gcccgacctg
     4081 tcgcagtggg ttgaactggt cgagaagatc aagatgccga aaaaggagct gtgggtagga
     4141 ctggtcggca agtacgtgga attgcccgat gcctatcttt cggtccgcga ggcgctcaaa
     4201 cacgctgcca ttttccacaa ccgggacctt agagtgcgtt acgtggattc ggagatgctg
     4261 gagcaggaag gcggcgagga gctgttgcgt ggattggatg gcatcgtcgt tccgggagga
     4321 tttggccagc gaggcatcga gggtaagatc agggcggcac gctacgctcg agagaatggt
     4381 gtgccctatc ttggattgtg cctggggttg cacgtgatgg ttgtcgagct ggcccgccac
     4441 gcgctaggga gcaacgacgc tcacagcact gaatgtgatc caaagacaat gcacccagtg
     4501 atccatctgc tgcccgagca gcggtcggtg cgggcaaaag gcgggacaat gcggctgggc
     4561 gcatacccat gccagatggt gttggggacc aaagcggcag cggcctatgg cgagggggag
     4621 atctcagagc gacatcgaca ccgctttgag ttcaataatg actacagggg aatgcttcag
     4681 gaggcaggcc tggtcatcag cgggttgtcc cccgacggca agctggtgga gatcgtggaa
     4741 ctgcgcgacc atccgtggat ggtcggcaca caggctcatc ctgagttcaa atctcggcca
     4801 acgaggccgc atccgctatt ccgcgacttc gtcgcggcgg cgaagcagac gctgcacgag
     4861 ggcgaccagc ggccactgcc gctggaccag atgctagcct agagccagaa gtagtggtga
     4921 aggaggagga gtagtagatg cgtttctttt tggacacagc caacatcgcc gagataagag
     4981 agacagcttt gatgggcatc atctgtggcg tgacgacaaa tccaagcctg atggcgaagg
     5041 agcagggggt cgacttcaag acggcggtgc aagagatctg cgcgctcgtc gacggacctg
     5101 tgagcgccga ggtcaccagc ctggaggtga cggggatgct cgaagaggcc cgcgagatag
     5161 tgggatgggc gcccaatgtc gtgatcaaga ttcctgtgac caaggcgggg cttcaggcta
     5221 ccagtgcgct gactaggcag ggaatcaagg tcaacatgac actgtgcttc accgccaatc
     5281 aggctctgct agccgccgcg gcgggggcca cctacgtgag cccgttcgtt ggccgtctgg
     5341 acgacatcgg gcaggacggt atgcagatgg tggcggagac cgtggccatc taccgaaact
     5401 atgggatcac agccggggat catgagcgct ggtgatgtac ctgaaactgg tgacgacctc
     5461 actcagaggc agcgcaccgt ttcattgcgc cactgtcaat tctcggaatc gctcgggttc
     5521 cttaattgga agaagtgtaa gggcaagccc cgacacggat gattcactgg ggaacccgac
     5581 cccttcactg gggccgcgca ttgaggagaa acaggatagc tcgattctag ttgcagccat
     5641 accctccagc gccgtcatta taccatgcct gtcaagcaac agtcaagtcg aatcacggct
     5701 tccccgctcc ttcgactctg gcagtccccg cccattgccc agcgaccagg ggcgagtccc
     5761 aataggatgt cgctctgcgg gttgagatcg atcagcccag tctcgcgcat cagtccagtg
     5821 ctgactaacg cgccttattc acactggccc agtccttgag aaaccggtct ataccggcac
     5881 tcgtcaacgg atgatcgacc atttgcatca ggactttaaa ggggactgta gcaatatgtg
     5941 cgccaaccct tgcggcggcc acaacatgca tcgtgtgccg gatgcttgcg gctatgacct
     6001 ccgttgtgat cccatagttt cggtagatgg ccacggtctc cgccaccatc tgcataccgt
     6061 cctgcccgat gtcgtccaga cggccaacga acgggctcac gtaggtggcc cccgccgcgg
     6121 cggctagcag agcctgattg g
//
LOCUS       DATALS010000063         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_92830,
            whole genome shotgun sequence.
ACCESSION   DATALS010000063 DATALS010000000
VERSION     DATALS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92830"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VHS06_01245"
     CDS             complement(<1..732)
                     /locus_tag="VHS06_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986787.1"
                     /translation="MKVTLFGSSIVSSYWNGAATYYRGICKALSKLGYELVFVEPDAY
                     QRQQHRDLLSDPEYIEVRVTRSWDELERELKAAQGSDLVAKCSGVGVFDDLLEERVAG
                     MAGDALTAFWDVDAPTTLEKAFSEPGWYFSKLVPKFDTILTYGGGPAVQKGYGTLGAR
                     AVHLVYNAVDPDEYAPAEPSEEYRCDLMLMANRLPDREERIKSIFFAAAELAPDQEFI
                     LGGEGWGDYPFPRNVRYIGHVPTALH"
     gene            complement(750..1940)
                     /locus_tag="VHS06_01250"
     CDS             complement(750..1940)
                     /locus_tag="VHS06_01250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2986788.1"
                     /translation="MSFDDLFWRPRRKMRVLMTTDAVGGVWTYTTHLVGALVNRDVQV
                     TMVSLGALPNKNQLRDLESLPGVELIPTPFKLEWMKDSEADVDASKRFIMDVAEQVKP
                     DIFHTNQFCFATLRLDVPKILVAHSDMLSWWSWRFHKGASDFAMEPFLVDYRQKVQRA
                     LLSADTVVCPSAFMAENLSRFYDPGLQRLCVIYNGIPTGDTSRDLRKKRRIAVSVGRL
                     WDEGKNAECVIQAARSLPDLEFLMIGPTKGPDGNKLKSTPPANVKLLGQLPPKTVWKL
                     LGSAGIYIGPSLYEPFGLAPLEAAVSNCALVLSDIPSFKEIWEDAALYFPPDAPDVLA
                     ERLRFMIDKPDVVCRHATMARFRAFRFYTTEKMAEQYHGLYVALISSAGGKVPLPQNI
                     QHAH"
BASE COUNT          359 a          668 c          648 g          399 t
ORIGIN      
        1 gtgcagggcc gtcggcacgt gccctatgta gcgcacgttt cgggggaacg ggtagtcgcc
       61 ccatccctcg ccccccagga tgaactcctg atcgggtgca agctccgcgg ccgcaaagaa
      121 gatcgacttt atgcgctcct cgcggtcggg gaggcggttt gccatgagca tcaggtcgca
      181 gcggtactcc tccgaaggct cggcaggggc gtattcgtcg gggtcgacgg cgttgtacac
      241 caggtgcacg gccctggccc cgagcgtgcc gtagcccttc tggacggccg ggccgcctcc
      301 gtaggtgagg atggtatcga acttcggcac cagcttgctg aagtaccagc cgggttcgga
      361 gaaggccttc tccagggtgg taggggcgtc gacgtcccag aaggcggtca gggcatctcc
      421 cgccatcccc gccacacgct cctcaaggag gtcgtcgaag acgcccaccc cgctgcactt
      481 tgccaccagg tcagaaccct gcgccgcctt cagctccctc tcaagctcat cccagctgcg
      541 ggtcacgcgg acctcgatgt actcgggatc gctgagcagg tcgcggtgct gctgcctttg
      601 gtaggcgtcc ggctcgacga acaccagttc gtagccgagt ttggagagcg ccttgcagat
      661 gcccctgtag taggtagccg cgccgttcca gtagctcgag actatgctcg agccgaagag
      721 agtcaccttc atcgatccac cccttgagct tagtgagcgt gctgtatgtt ctgcggcagg
      781 gggacctttc ccccggcgga ggatatcagg gccacgtaca ggccgtggta ctgctcggcc
      841 atcttctcgg tggtgtagaa cctgaaggcc ctgaacctgg ccatggtcgc atgccggcag
      901 acgacgtcgg gcttgtcgat catgaagcgc aggcgctcag ccaacacatc cggtgcgtcc
      961 ggcgggaagt agagcgccgc gtcctcccag atctccttga acgacgggat gtcgctcagg
     1021 accagggcgc agttggagac ggctgcctcc agcggggcca gcccgaatgg ctcgtagagg
     1081 gaggggccga tgtagatgcc cgcgcttccg agaagcttcc agacggtctt gggcggaagc
     1141 tgtcccagca gtttgacgtt ggccggcggc gtgctcttga gtttgttccc gtcgggccct
     1201 ttggtcgggc cgatcatcag gaactccagg tcgggcaggc ttcttgccgc ctggatgacg
     1261 cactcggcgt tcttcccctc gtcccacagc ctcccgacgc tgacggcgat ccgcctcttc
     1321 ttgcgcaggt cgcgggaagt gtcccccgtg gggatgccgt tgtagatcac gcacaggcgc
     1381 tgcagtccgg ggtcgtagaa cctggaaagg ttctccgcca taaaggccga cgggcagacc
     1441 acggtatcgg ccgaaaggag cgccctctgg accttctgcc ggtagtccac gaggaacggt
     1501 tccatggcaa agtcgcttgc acccttgtgg aacctccagc tccaccagct gagcatgtcg
     1561 ctgtgggcca ccaggatctt cggcacgtcg aggcggaggg tggcgaagca gaactggttg
     1621 gtgtggaaga tgtcgggttt gacctgctcg gcgacatcca tgatgaacct tttggaggcg
     1681 tccacgtcgg cctcggagtc cttcatccac tccagcttga acggcgtggg gatcagctcc
     1741 actccgggaa ggctctccag gtcgcggagc tggttcttgt tcggcagcgc gcccaggctg
     1801 accatcgtga cctgcacgtc ccggttgacg agggccccca cgaggtgcgt ggtgtaggtc
     1861 cacacacctc cgacagcatc cgtggtcatc agcacgcgca tcttccttct gggcctccag
     1921 aaaagatcat caaagctcat ctcaaaactc ctgtgcccct catcccaacc ttctccggca
     1981 aggggagaag gaaagataca tcgttccttc gggacacccc tgtcccgaag cttcaccttc
     2041 ctcaggcaac agcaacagac actccctccc ccct
//
LOCUS       DATALS010000064         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_230698, whole genome shotgun sequence.
ACCESSION   DATALS010000064 DATALS010000000
VERSION     DATALS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230698"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VHS06_01255"
     CDS             <1..370
                     /locus_tag="VHS06_01255"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX2986789.1"
                     /translation="DGVPVAHYVADGLIVSSATGSTAYALSAGGPVMAPDIDAMVVVP
                     IAPHLAVLRSLVVPGSSRVEIRASADQPTLLVLDGQVQIPLKNNQLVSVGVAPERTVF
                     ARTGARSRFYETLLEGFKKR"
     gene            380..>1929
                     /gene="recN"
                     /locus_tag="VHS06_01260"
     CDS             380..>1929
                     /gene="recN"
                     /locus_tag="VHS06_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX2986790.1"
                     /translation="MLTELSIRDFAIIDELRVQFAHGLNVLTGETGAGKSIIVDALGA
                     TLGQRVDSDVVRTGANLASAEAVFEVDCGDADGALATLLAEHGLEAEDGLLVLRREIN
                     RSSGRTVGRVNGRVVPVSVMQRVGQLLVDIHGQTDHLSLLRVREHVDYLDRYAGLMEQ
                     RRRLSEAVRELRAVRDDIEKMTKDERESAREMDLLGYQIQEIGNAGLTADEEERLLSE
                     RSRLRNAERLMEAAAQLHVLLSGGDEQVGAIDLLAQAQKQYLELARYDPSQEADREAL
                     DTAVFAVEEAAQRARSYAESIENDPARLTEVEERLEVISNLKRKYGRSVAEVLAYCEE
                     AEAKLEKLSNRAFYLDGLREAEHGLLEKVGELAGSVSRQRKATAVRLAEEMHRELRDL
                     NMGGTSFQVSFSVSEDPNGVAYLPVSCHPSALTPTLAQREREHSVASAQQVGGESERV
                     GFDATGIDRVEFLVSPNPGEEPKPLAKIASGGETARLMLALKTILSKADAIPTLVFDE
                     IDVGVGAR"
BASE COUNT          348 a          478 c          761 g          342 t
ORIGIN      
        1 ggatggggtg ccggtggcgc actacgttgc cgatgggctg atcgtctcga gcgcgaccgg
       61 ctcgacggca tacgcgctgt ctgcgggagg gcccgtgatg gctccggaca tcgacgcgat
      121 ggttgtggtc ccgatagcgc cccatctggc cgtgctgcgc tcgttggttg tgccggggtc
      181 gtcgcgggtg gagattcgcg cttcagccga tcagccaact ctgttggtgc tggacgggca
      241 ggtgcagata ccgctgaaga acaaccagct ggtgagcgtt ggggttgctc cggagcggac
      301 ggtatttgcc cgaacgggtg cccgatcgcg gttctacgag acgttgctgg aagggttcaa
      361 gaagaggtag gagcaaccat tgcttaccga gctttccatc cgagacttcg ccatcatcga
      421 tgagctgagg gtgcagtttg cgcacggtct caatgtgctg accggcgaga ctggggcggg
      481 gaaatcgatc attgtggatg cgctgggggc aacccttggg cagcgggtag actcggacgt
      541 ggtgcggacg ggagccaatc tggcgagtgc cgaggcggtg ttcgaggtgg attgcggtga
      601 cgccgacggc gccttggcta cccttctggc cgagcacggg ctggaggccg aggatgggtt
      661 gctggtgctg aggcgcgaga tcaaccgctc gtccggccgg acggttggga gggttaatgg
      721 gcgggtggtg cccgtgtcgg tgatgcagcg ggtggggcag ctgttggtgg acatccacgg
      781 gcaaaccgat cacctctccc tgttgcgagt gagggagcat gtggactacc tggaccgata
      841 tgccggtttg atggagcagc gcaggcggct atcggaggcg gtgagggagt tgcgcgccgt
      901 ccgggacgac atagagaaga tgacgaagga cgagcgggag tcggcgcgag agatggatct
      961 cctgggctac cagatccagg agataggcaa cgctgggctg acggccgatg aggaggagcg
     1021 gctcttgtcg gagcggagcc ggctccgaaa cgccgaacgg ttgatggagg cggccgccca
     1081 gctacatgta ctgctgagtg gtggggatga gcaggtgggc gcgatagacc tgctggctca
     1141 ggcgcagaag cagtaccttg agctggcgcg ctacgacccg tcgcaggagg cggacaggga
     1201 ggcactggat acggcggtct tcgccgtgga ggaggcggcc cagcgggcaa ggagctacgc
     1261 ggagtcgata gagaacgatc cggcgcggct gaccgaggtg gaggagcggt tggaggtgat
     1321 ctccaacctg aagcgaaagt atggccggtc cgtggcagag gtgctggcgt actgcgagga
     1381 ggccgaggcg aagctggaga agctctccaa tcgagctttt tatctggatg ggctgcgaga
     1441 ggcggagcac gggctgctgg agaaggttgg tgagctggca ggttctgtgt ctcgtcagcg
     1501 gaaggcgacg gcggtcaggc ttgccgagga gatgcacagg gagcttaggg acctgaacat
     1561 gggcgggacc agcttccagg tgtcgttttc tgtatcggag gatccgaacg gggtggcgta
     1621 cttgccggtc agctgtcatc cgtcggccct caccccgacc ctcgcccaga gggagaggga
     1681 gcactcagta gccagcgccc agcaggttgg tggcgagtcg gaacgggttg gtttcgatgc
     1741 gacaggcatc gacagggtgg agtttctggt ctcaccgaac cccggggaag agccgaagcc
     1801 gttggcgaag atagccagtg ggggagagac cgcgcggttg atgctggcgc tgaagacgat
     1861 cctgtcgaag gcagacgcga tacccacgct ggtgttcgac gagatcgacg tgggggttgg
     1921 ggccaggag
//
LOCUS       DATALS010000065         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_139960, whole genome shotgun sequence.
ACCESSION   DATALS010000065 DATALS010000000
VERSION     DATALS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139960"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..957
                     /locus_tag="VHS06_01265"
     CDS             124..957
                     /locus_tag="VHS06_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2986791.1"
                     /translation="MTRFPIWRLAGAAVGLALLDAVAFEPSRLRVQRLMVPSAGSGSR
                     KASLRLAQLSDLHVGGRGWRHDVLARAIDVCNREDVDAIVITGDLIGSEAGADEVLRA
                     IALLRSDVPRLVVFGNHDHVHGGRALAHLGRGLDRLGMAVLRNDVVRLELPAGPVWFA
                     GVDDGYSMRDRLELVREKMAGVTEPRILLTHYPDVADRLRSGEFQLSLAGHSHGGQIR
                     MPIVDTLVCNSHARTRYARGLYIVNENPLYVSAGLGMSGVPMRFRNWPEMSLIYVPLI
                     Y"
     gene            1204..>1652
                     /locus_tag="VHS06_01270"
     CDS             1204..>1652
                     /locus_tag="VHS06_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX2986792.1"
                     /translation="MKRLLVSLVAVLALASLVVTGCSQSAPAPAPTKAAEPAKAAEPT
                     KAAAPAAQPTAAAPAAASKTSWPEKGKSITCIVASDAGGSSDVGARLISAYIEKELGV
                     SIQVVNKPGAGWQVGLTELSKAKPDGYTFGMAVMPQANTIYLDPERKA"
BASE COUNT          300 a          495 c          535 g          322 t
ORIGIN      
        1 ttcgctgaag cgcgaggcga aactccaaca gtgtaggggt aacgcatacg ccgcacctac
       61 gggaagttgc cccagttgac catcggcgcc gagtgatgac agtcggatca gtagaggttg
      121 aggatgacac gtttccccat atggcggttg gccggcgcgg cagtcggtct cgcgctgctg
      181 gatgccgttg ccttcgagcc ctctcgactg agggtgcaga ggttgatggt tccctcggct
      241 ggaagcggct cgcgaaaggc gagtctgagg ctggcgcaac tgtcggatct gcacgtcggg
      301 ggcaggggct ggcgacatga tgttctggct cgtgccatcg atgtgtgcaa ccgggaggac
      361 gtggacgcca tcgtcatcac cggcgacctg atcgggagcg aggccggcgc agatgaggtg
      421 ctgagggcga ttgccctcct tcgaagtgac gtacccaggc tagtagtgtt cggcaaccat
      481 gaccacgtgc acggcggaag ggccctggca caccttggtc gcggtctcga tcgcctgggg
      541 atggccgttc ttcgaaacga tgtcgttcgg ttggaattgc ccgcaggtcc ggtatggttt
      601 gccggagtcg acgatggcta cagcatgcgt gaccgcctgg agttggtgag agagaagatg
      661 gcaggtgtca ccgagcctcg gatcttgctg acccactatc cagacgtggc cgaccggctg
      721 aggagcggtg agttccagct atcgcttgcc ggacactccc acggtggaca gatccggatg
      781 ccgatcgtgg acaccctcgt gtgcaacagc cacgcgcgca cccgctatgc gcggggcctc
      841 tacatcgtga acgagaaccc cctctacgta agcgccggac tcgggatgtc cggcgtgccg
      901 atgcgtttca ggaattggcc tgaaatgagc ttgatctatg tgcctcttat ttactaaact
      961 gatgatgttc ctaacccctt acggctcatc gtggtagcat ggaccgcgag attctcggga
     1021 cactggtgcc gcgatggccg gaatgggcga tcacaggacg tgccccagga ttgagcttgg
     1081 tcgtgtagtc gttcccttgc gtttggtcgt tgagaatccc ccggaattca ttttgccggc
     1141 atgcatcgcg atccccagga attggtgcac cgtcgtgcca aggccgaatc gataggagga
     1201 gttatgaagc gactactcgt atccctggtt gccgtactcg ccctcgcgag cctggtggtg
     1261 accggttgct cgcagtccgc acccgctccc gctcccacga aggcggctga acctgccaag
     1321 gccgccgagc ccaccaaggc cgcggctccc gccgcgcagc cgaccgccgc cgctcccgcc
     1381 gccgcatcga agaccagttg gccggagaag ggcaagtcta tcacctgcat cgtggcctcc
     1441 gatgccggtg gctccagcga cgtcggcgct cgactgatct cggcctatat cgagaaggag
     1501 ttgggtgtat cgatccaggt cgtcaacaag ccgggcgcag gctggcaggt cggtctcacc
     1561 gagctttcca aggccaagcc cgatggctac accttcggca tggcggtcat gccccaggcc
     1621 aacaccatct acctggatcc agagcggaag gc
//
LOCUS       DATALS010000066        10797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_5616,
            whole genome shotgun sequence.
ACCESSION   DATALS010000066 DATALS010000000
VERSION     DATALS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10797
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5616"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VHS06_01275"
     CDS             <1..362
                     /locus_tag="VHS06_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2986793.1"
                     /translation="IEDDLAVLELLGSVLSEAGYRPILIPNSERIPEKVTREKPDLIL
                     LDILLQPAHGMEVLSAISKLEHRPPVIMMSAAIKGVGDMVHIARGLGAEDFIEKPFDI
                     IDLLARIETVLDRKMVS"
     gene            515..1717
                     /locus_tag="VHS06_01280"
     CDS             515..1717
                     /locus_tag="VHS06_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX2986794.1"
                     /translation="MARALRGKLLVGQSGGCTAVINASLVGVIEEASRHSDIEGVLGT
                     LHGVEGVLRGELVDLASESEETLRRLRRTPSSALGSCRYKLHEGDLERIVHTLRENDV
                     RYFLYIGGNDSADSSHKIANAAAEIGYDLRVMAVPKTIDNDLPITDHCPGYGSIARFV
                     AQSTQDAGLDTEAMKRYDPVKIIEVMGRDAGWVAAASALGKSSLQDAPHLVYLPERTF
                     DVQRVLEDVKRVHHEVGYVVVVLSETVRDAKGQVVGSEKPYFVDPFGHGYYESPAAYL
                     CRVVARELGLRARFDKPGTIQRMSMALASPTDLEEAYLVGSQAVRYALQGRTDQMVIL
                     VRVPGTHYRCTTDSVPLEQIANVVKPLPPEFISPEGNFVTPAFMDYALPLIGGPLLQY
                     ARLAKRPI"
     gene            1760..2614
                     /gene="mutM"
                     /locus_tag="VHS06_01285"
     CDS             1760..2614
                     /gene="mutM"
                     /locus_tag="VHS06_01285"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEX2986795.1"
                     /translation="MPELPEVETIKNDLRSTVIGRTIETVRVLDPRLVHYPSLGAFRS
                     GLEGQTILAVDRRAKYLLLRLSSDRMLVIQLMLTGQLLLVQPERPLVKSTRMILDLDG
                     DEQLRLVDSIHFARVNLVDAEELDRLLHLRELGPEANSPELTLERFADLIRSHNRQIK
                     PLLLNQRVISGLGNIYTDESLFAARIHPLRRASSLTPLEIENLYHAMREILREAVALR
                     GTTTRSYRDVGGEKGQFQQRLKVAVRAGKPCIGCPGVVERIVVGGRETYICPSCQRLE
                     APAERRAA"
     gene            complement(2653..4194)
                     /locus_tag="VHS06_01290"
     CDS             complement(2653..4194)
                     /locus_tag="VHS06_01290"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX2986796.1"
                     /translation="MEAQTSTSKAGGATRPLPLLFSVFAVATCAIVYELMIASISSYL
                     LGDSIYQFSVTVGLFLTTMGVGSYLSRRVHRNLLLTFIAVELVIGAVGGSSTLLLYYV
                     YGNAKDVYQPAMYLVIAVIGTLAGLEIPIVTRLLSEKFELRINIANVLSFDYLGGLLG
                     SLAFPLLLLPFLGLLRTALAVGLVNAGVAGLVLVAYWRQLPERRYVAVLVATVVGLLA
                     TAMWDSGNLWAQLEQRLYRDPVVFSEQTPYQHITITQWRDDLRLFLDGNLQFSSFDEY
                     RYHEPLVHPAMAAARDRSEVLILGGGDGLAVREALKYTEVKRVTLVDLDPQMVSLFRT
                     QPQLTRLNGDSLNDPRVRVVEGDAFKFLEDDNGFYGVIIADLPDPRNENIQKLYTVEF
                     YRLASRRLAVGGAFVTQATSPYFAPEAYWCIYRSMKEVWPQVAPYHAQVPSFGDWGFL
                     LATSRPVDPSTLAPKVSTRFLDGPTAAALFVFPADMPRLQMSPNSLIQPVLPGYYRQG
                     WRQAR"
     gene            complement(4290..4502)
                     /locus_tag="VHS06_01295"
     CDS             complement(4290..4502)
                     /locus_tag="VHS06_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX2986797.1"
                     /translation="MEVGAFLNSVLSSVVWAVLGVLLLILAFRIFDLSDPVRYHTEIE
                     KGNVSAGIVMAGVMIGMSIIIFAAIR"
     gene            complement(4528..4665)
                     /locus_tag="VHS06_01300"
     CDS             complement(4528..4665)
                     /locus_tag="VHS06_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986798.1"
                     /translation="MEPWGLGSQQLPIVVRQFVQQQLVIQVELVFGRQEQRRQHQWWR
                     N"
     gene            complement(4805..5353)
                     /locus_tag="VHS06_01305"
     CDS             complement(4805..5353)
                     /locus_tag="VHS06_01305"
                     /inference="COORDINATES: protein motif:HMM:NF022389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2617 family protein"
                     /protein_id="HEX2986799.1"
                     /translation="MRYVEQCSTDLCYYLLEGAAPRWAVRVLREQTIQLANGRLTLAI
                     VGSSHFLEMETGRVALSELLACPTDAMAKMECSHRLSETASCNRQYRTAGLAYDFRIW
                     KDRLEERAFEAESRRLMKHTERRLSYSFPKSREAVSAVTCLDWQIDDCVATVATHHTF
                     PGELAVVHTRSVIDFSEVVARQ"
     gene            complement(5407..5955)
                     /locus_tag="VHS06_01310"
     CDS             complement(5407..5955)
                     /locus_tag="VHS06_01310"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEX2986800.1"
                     /translation="MSWLKRLIGEDEPEKPEERTPQTIQVGDGVSYMGEDYIVEQRIE
                     YHGDGGDIWWDFLLVGPEDKYWLSVVEDEGLEIALYHDIPFHPDMPPAKTLAWKGESY
                     YRKEYGFADAIIKKKSRLTTTGRVEYWDYENEEGKLLCVERWGDNEVREDQSEMTGTI
                     GCSLGETIKPYQVKIYPGTQQE"
     gene            6241..6317
                     /locus_tag="VHS06_01315"
     tRNA            6241..6317
                     /locus_tag="VHS06_01315"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6275..6277,aa:His,seq:gtg)
     gene            6330..6403
                     /locus_tag="VHS06_01320"
     tRNA            6330..6403
                     /locus_tag="VHS06_01320"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6364..6366,aa:Arg,seq:tcg)
     gene            complement(6711..8822)
                     /locus_tag="VHS06_01325"
     CDS             complement(6711..8822)
                     /locus_tag="VHS06_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX2986801.1"
                     /translation="MRLSVRTRLLAGYVVVLILAGILGTMAVTRTNSLSDMLNATYHS
                     QLLPVAYTGDIQNAVMTRARDLRNVIIFAKDETKRSAALNSIAAADQQISKSLAKYKD
                     TAPTEQEKQLLASFESAYVDYKNVADDIAKTAIQGQVDTAIGRLDEAAVSVGKVEKVA
                     EQLKQHYADTASKEDDLGDQEAAQTRVIVIGLLAVVILVGLTTGIALSHDLSRSVATI
                     HLVLERLAVGDLNRDMDERTKDKLRNRSDELGDIGKALTKTIVYMRAMAEHAQQIARG
                     NLTEVVTPLSDKDELGLAFEEMVTDLRQIVGGVASSARALEEASHQLSSSSGQAGDAT
                     QQIATTIQQVASGTQDQSSAAQDTASSVDQLSRAIDQIAAGAQDQSRAIEKASASVAQ
                     LNTSITEVTESSQEVSAATTQAQDAAQAGAKSVEKTANGMAAIKASTASVAGKIEELG
                     RYSEQIGSIVQTIDDIAEQTNLLALNAAIEAARAGEHGRGFAVVADEVRKLAERSSKS
                     TKEIADLIGHVQKGTQDAVVAMGQEAKEVESGASLAEEAAKALSNILTAVKSASNQVS
                     NIANAVKQMEAASQEVVGLMDSVSAVVEESSAATQQMAASSQQVSQAIERVASVSEQT
                     SAAAEEVSASTEEMSAQVEEMVAQAQTLDGLAKELHAAISRFNLGNQNERSVQPLKLD
                     ARAAQPSGTARLVGFPASAAR"
     gene            complement(9257..9592)
                     /gene="flgM"
                     /locus_tag="VHS06_01330"
     CDS             complement(9257..9592)
                     /gene="flgM"
                     /locus_tag="VHS06_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEX2986802.1"
                     /translation="MTGSDPISSDQSVHGRIIGPSQSRPPDSDSAARLSKRLLNSTQA
                     DQVSISTDDARLASKLHDAVRASSDVRESRIEEIRAAIANGTYQVDPREIAQAMLSPN
                     HTRKTVEKQ"
     gene            9852..>10797
                     /locus_tag="VHS06_01335"
     CDS             9852..>10797
                     /locus_tag="VHS06_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986803.1"
                     /translation="MDRSEKRALHSSFWEMGPSERPLGSCWIGSRMPDEIYRATRSIP
                     IGKVMPSDVSVEDFMDDYDRLYQMHEAVGDDAFWSASAFYGIPWVEAILGCPVYYSGD
                     SFWVEPIFPEWPERLDLPRPEGQEWLAKLLDFTEALVRHADGRYAVTATLMRGPSDLI
                     AAARGHLEMIYDMYDHPEQLERLAMVATDLWIQVARRQLDLIPRYEGGYVSHFYRIWA
                     PEWVVCPQEDASASFSPEFFNRILMPADEKISSEFPYSVMHLHSPTTWPVEQLLEIDR
                     LSCIEINYDDNGPRLSELMPVLQRIQQVKPLIIRGALTV"
BASE COUNT         2032 a         3161 c         3304 g         2300 t
ORIGIN      
        1 tcatcgaaga cgacctcgcg gtcctggaac tgctgggttc ggtactatca gaggcgggct
       61 atcgcccgat tctcatcccc aacagcgagc ggatccccga gaaggtgacg cgggagaagc
      121 cggacctgat cctgctggac attctgcttc agcctgcgca cggaatggag gtgctatccg
      181 ccatctccaa gcttgagcat cgcccaccgg tgatcatgat gtccgcggcc ataaaggggg
      241 tgggtgacat ggtgcacata gcccgggggc tgggagccga ggacttcatc gagaaaccct
      301 tcgacatcat cgatttgctg gcgcgcattg agacggtgct ggacaggaag atggtctcct
      361 aagcgacttt cccttgttgg tgtccacttt cctctcatgg aaaggtcgac cgcatccagg
      421 aaaagacgct tagccctttc gtcgcatggg gctctgcgga tacaatggcg cggcatcggg
      481 accggagtca gcgaagattg ggagggggac gcggttggcc agggcgttac gtggaaagct
      541 gttggtggga cagtcgggcg gctgcacggc cgtcatcaat gccagtcttg ttggggtcat
      601 cgaagaggct agtcgccata gcgacatcga gggtgtgttg ggcaccctgc acggtgtaga
      661 gggggtgctg agaggcgagc tagtcgacct cgcttcggag agtgaagaga cactacggcg
      721 gctgcgccgc accccgtctt cggctctggg gtcatgccgt tacaagcttc acgagggaga
      781 cctggagcgc atcgtccaca ccctgcggga aaatgatgtt cgttacttcc tctacatcgg
      841 cggcaacgac tctgccgaca gctcccacaa gatcgccaac gccgccgccg aaattgggta
      901 cgacctccgg gtgatggcgg tccccaagac gatcgacaat gacctcccca tcacggacca
      961 ctgtccgggc tacggttcca tagcgcgctt cgttgcacag agcactcagg acgcgggact
     1021 ggacaccgag gcgatgaaac gctatgaccc ggtaaagatc atcgaggtga tgggacggga
     1081 cgcggggtgg gttgccgcgg ccagcgcatt gggcaagagc agcttgcagg atgcccccca
     1141 cctggtgtac ctgccggagc gcaccttcga tgttcagcgc gtcctcgagg atgtgaaacg
     1201 ggtccaccac gaggtgggct acgtggtagt ggtgttgagc gagacggtga gagacgccaa
     1261 ggggcaggtg gttgggagcg agaagcccta cttcgtggat ccttttggcc acggttacta
     1321 cgagagcccc gctgcctacc tttgccgtgt agtggcccgc gagcttgggc tgcgcgcacg
     1381 gttcgacaag cccggtacca tccagcgaat gtccatggca ctggcatcac cgacggatct
     1441 cgaggaggcg tatctggtcg gcagtcaggc ggtgcgctac gcgctacaag ggcgtaccga
     1501 ccagatggtt atcctggtga gggtgccggg gacccactac cgatgcacta ccgactcggt
     1561 tccgctggag cagattgcca acgtggttaa gccgctgccg ccggagttca tctcccccga
     1621 gggcaacttc gtaactcctg ccttcatgga ctacgccctt cccctgatcg gaggccctct
     1681 gctgcagtac gcacgactgg ctaagcgacc gatctagccg cgtggcacag ggggtgcctg
     1741 gcctcggcag gagggtgtta tgcctgaact gcccgaggtc gagacgatca agaatgacct
     1801 gcgatctacg gtcatcggaa ggacgatcga gactgtcagg gtcctggacc cgagactggt
     1861 gcactacccc tcgctgggcg cattccgatc gggactggag ggacagacta tcctcgcggt
     1921 ggaccggcgg gccaagtacc tcctcctccg tctttcatct gacaggatgc tcgtgatcca
     1981 gttgatgcta accggccagc ttctgctggt tcagccggag cgtcccctgg taaagtccac
     2041 gcgaatgatc ctcgatctgg atggggacga gcagctcagg ttggtggaca gcatccactt
     2101 tgccagggtg aacctggtag acgccgagga gttggaccga ctgctgcacc tgcgggaact
     2161 tgggcccgag gccaactccc ctgagttgac cctggaacgt tttgccgatc tgatccgcag
     2221 ccacaaccgc cagatcaagc cgctactgct gaatcagagg gtgatctccg gtctgggcaa
     2281 tatctacacc gacgagtcgc tcttcgcggc ccgcattcac cccctcaggc gagcctcctc
     2341 gctgacccct ttggagatcg agaacctgta tcatgccatg agggagatac tgcgggaggc
     2401 agtggcgctc cgagggacca ctacccgttc ctatagggat gtcggcggcg agaaggggca
     2461 gttccagcag cggctcaagg tcgctgtcag ggccggtaag ccctgcatcg gatgccccgg
     2521 agtggtggag aggatcgtcg tgggtgggcg ggagacttac atttgtccct cctgccagcg
     2581 attggaggca cccgccgaga ggcgcgctgc ctgacggcag gacgcgggga cgcgtgtccc
     2641 cgtgtctccg tgtcacctcg cctggcgcca gccctggcgg tagtagcccg gcaggacggg
     2701 ctgtatgagg ctgttggggg acatctggag ccgaggcata tccgctggga agacgaagag
     2761 cgctgcagcg gtgggaccat ccaggaagcg ggtagacacc ttgggcgcca gggtggatgg
     2821 atctaccggg cgcgatgtag ccagcaggaa accccaatcc ccgaaggacg gaacttgagc
     2881 gtggtagggg gccacctgcg gccacacctc cttcatggac cggtagatac accagtacgc
     2941 ctccggcgcg aagtagggtg aggtagcctg ggtgacgaaa gccccgccaa ccgcgaggcg
     3001 tcgtgatgcg aggcgataga actccacggt gtacagcttc tgtatattct cgtttcgggg
     3061 gtcgggcagg tcggcaatga tcaccccgta gaagccgttg tcgtcctcca ggaacttgaa
     3121 ggcatccccc tcgaccacgc ggaccctggg atcgttcaag gagtcgccgt tcagccttgt
     3181 tagctgaggc tgtgtcctga acagcgagac catctgagga tcgagatcca ccagagtcac
     3241 ccttttcact tcggtgtact tgagcgcctc cctgacggcc aggccgtcgc cgccaccaag
     3301 tatcagcacc tcggatctat ctctcgctgc cgccatggca gggtgtacca gcggctcgtg
     3361 ataccggtac tcgtcgaagg agctgaattg tagattccca tccaggaaga gccgtaggtc
     3421 gtctctccac tgggtgatgg tgatgtgctg atagggagtc tgctccgaga atacgacggg
     3481 atcgcgatag agtcgctgct cgagctgggc ccacaggttg ccgctgtccc acatggcggt
     3541 ggccaggagc cctaccacgg tagccaccag tacggcgacg tatcgccttt cggggagttg
     3601 ccgccagtag gccaccagca ccaagcccgc gacgccggcg ttcacgagcc cgacggccag
     3661 cgcagtccgt aacagcccga ggaacgggag cagcagcagc ggaaaggcga gggagcccag
     3721 aaggccgcca aggtagtcga aggagaggac gttggcgatg tttatccgca gttcgaactt
     3781 ctcggacagt agcctggtga ctatggggat ctcgaggccg gccagagtgc cgatcaccgc
     3841 gatcacgagg tacatggccg gctggtatac gtctttggcg ttgccgtaga cgtagtagag
     3901 caggagcgtg gagcttcccc ccacagctcc aatcaccagc tcgaccgcga tgaaggtgag
     3961 cagcaggttc cggtggacac ggcgggagag gtatgatccc acacccatcg tggtcaggaa
     4021 gaggccgacg gttacagaga actggtagat agaatcgccc agtaggtacg agctgatact
     4081 cgcgatcatg agctcgtaca ctatggcgca ggtcgccacg gcgaataccg agaataggag
     4141 aggcagggga cgggtcgcgc cccctgcctt agaggtgctg gtttgtgctt ccatgatgtt
     4201 ctcagctctt agctatcagc cacgagcaat cagcgcgcga aagtcatcgg ctgaaagccg
     4261 gtagctgatg gccgagagct gaaagctttc tagcgtatcg ccgcgaagat gatgatggac
     4321 atgccgatca tgacgcctgc catgacgatg cccgcggaaa cgttgccctt ctctatctcc
     4381 gtgtggtagc gcaccggatc gctcaggtcg aaaatcctga aggctagaat gagaagcaga
     4441 accccaagaa ccgcccatac taccgacgag aggaccgagt tcagaaatgc accaacttcc
     4501 atgggttact tacctccact tcctcctcta gttccgccac cactgatgct gccgccgctg
     4561 ctcctgcctg ccgaagacga gctcgacttg gatgacgagc tgctgctgga cgaactgccg
     4621 gacgacgatg gggagctgct gcgatcccag cccccacggt tccatccgcc accccctatg
     4681 aagactggag caccccagga tggcccaaag aaacctcctc ccaccctggg ggtgcaggca
     4741 ttggcgaggg cagagaacca gatcagcgct atgaacagta tgatggccat gagaactagt
     4801 cttttcattg tcgggccacc acctccgaaa aatcgatcac cgagcgagtg tggaccacgg
     4861 ccagttcgcc agggaaggtg tgatgggttg ctaccgtggc cacgcagtca tcaatctgcc
     4921 aatcgaggca ggtgacggcg ctgactgcct ctcgcgactt agggaagctg taactgagcc
     4981 gccgttccgt gtgcttcatc aaccgcctgc tctcggcctc gaacgcccgc tcttcgagcc
     5041 tatccttcca gattcggaag tcgtaggcga gcccggcggt tcggtattgg cggttacaag
     5101 aggcagtctc ggatagacgg tggctgcact ccatttttgc catggcgtcg gtagggcagg
     5161 cgagtagttc gctcaatgcc accctgccgg tctccatctc caggaagtga ctgctgccga
     5221 cgatggcaag ggttagcctg ccgttggcta gttgaatggt ctgctctctg agtacccgca
     5281 cggcccatcg cggggcagcg ccttccagca ggtagtagca caggtcggtc gaacactgct
     5341 cgacataacg cacttggtcc gaggacagag gccctcccgc cctgtggggc aggatgggac
     5401 ctgcacttac tcctgctgag tgcccgggta tatcttgacc tgataaggtt tgatagtctc
     5461 gccgagagag catcctatgg ttccagtcat ctcggactgg tcctctctga cttcgttgtc
     5521 gccccagcgc tccacgcaga gcagcttgcc ctcttcgttt tcgtagtccc aatactccac
     5581 cctgcccgtg gtggtgagtc tggacttctt cttgatgatg gcatcggcga agccgtattc
     5641 cttgcggtag taggactcac ctttccacgc aagcgtcttt gccggaggca tgtccggatg
     5701 gaatggtatg tcgtggtaca gggcgatctc cagcccctcg tcctccacga ccgagagcca
     5761 gtacttgtct tctgggccta ccagcaggaa atcccaccag atatccccgc cgtcgccgtg
     5821 gtattcgatc cgctgttcga cgatgtagtc ctctcccatg taggagactc cgtcgccgac
     5881 ctgaatggtc tggggagttc gctcttcggg tttctcgggt tcgtcttcgc cgatgagcct
     5941 cttgagccag ctcatagggg gtcctccgcg actagatcag gtaaagatgc acagtttgga
     6001 gaagcatatc acctctgggg tgcaagtcaa ggctaagtag ctgtagatca tccctagcga
     6061 tgcgtgccga cgggcacaac ttgccccaca tcggtttaga ccagggtgtg ggagctcttg
     6121 tttcacagcc tcttcaggat ggactcctac ggccgcgaag aatgccatgg ctgttcatgc
     6181 gtgttgccct ttgggcggcg ggactggtat aatacgcttc cgcggggtga tccgcgtatg
     6241 gtgagggtag ccaagtggtt taaggcgcca ggttgtggcc ctggagatcg agggttcgaa
     6301 tccctttcct caccccaatc gccgcataag cgcccgtagc tcagtggatt agagcactgg
     6361 tcttcggaac caggtgtcgg gggttcgaat cccttcgggc gcgcccttca aaacgtggca
     6421 aatggggcct ggactcaagt ccaggcccct cttttcttgc aacatcatcg cccgtgccag
     6481 aatgctccgg cacgggcgat gatgcctttc gatccgtttt gagaggagtt ccgcttctct
     6541 tgaaggggtc tcggggttgc cctcacaaac cgcgcaccgt tgcgagtctc gcaccagttg
     6601 ccctcgcgtc gtgccttccg tgacctcaca atgaggatcg atcgtagtga acgggtctct
     6661 tctcctctct cctggctggt acttcccctc ttcttgactt gaatgatcgt ctatctagcc
     6721 gcggatgccg gaaatcctac taatctggcg gtgccggatg gctgagctgc cctagcatcg
     6781 agcttgaggg gctggacgga tctctcgttc tggtttccca gattgaagcg actgattgcc
     6841 gcgtggagtt ccttggccag cccatccagc gtttgggcct gggcaaccat ctcctccacc
     6901 tgggcactca tctcctccgt ggaggcactt acctcttcgg cagcggcgct ggtctgttca
     6961 ctcacggaag ccactcgctc tatcgcctgt gacacctgtt ggctggaagc agccatctgt
     7021 tgtgtggccg ccgaagactc ttccaccact gccgacacag agtccatcag gcctacgact
     7081 tcctgagacg cggcctccat ctgcttcacc gcgttggcga tgttagatac ctggttgctg
     7141 gcggacttga cggcagtcag gatgttgctc agagccttcg cggcctcttc ggccaacgac
     7201 gcccctgatt caacctcctt cgcctcctgc cccatggcga ccaccgcgtc ctgggttccc
     7261 ttctgaacgt ggccgattag atcggcgatc tccttggtgg acttgctgga tcgctcggcc
     7321 agcttcctga cctcgtctgc cactaccgcg aatccgcggc cgtgttcccc tgcccgagca
     7381 gcctcgatcg cggcgttgag tgccagcagg ttggtttgct cggcgatgtc gtcgatggtc
     7441 tgcactatgg agccgatctg ctcggagtat cggcccagtt cctcaatctt gccggccacg
     7501 gatgccgtgc tggccttgat tgcggccatt ccattggccg tcttctcgac ggattttgcg
     7561 cccgcctgtg cagcgtcctg ggcctgcgtc gtcgctgccg aaacctcttg agaggattcc
     7621 gttacttcag tgatcgatgt gttcagttga gctaccgagg cagaggcttt ctcaatcgcg
     7681 cggctctgat cctgggcgcc cgcggcgatc tggtcgatgg cgcgagagag ttggtctacg
     7741 gatgaggctg tgtcctgtgc cgcgctcgat tgatcctgcg tgccggacgc gacctgctgg
     7801 attgtggtcg cgatctgctg ggtggcatcg ccagcctgcc ccgatgagga cgagagttga
     7861 tggcttgcct cttccagtgc ccttgcggat gaggcaactc cccctactat ttggcggagg
     7921 tcggttacca tttcttcgaa ggcgagaccg agctcatcct tgtcggaaag gggcgtgacc
     7981 acctccgtca gattgcccct cgcgatctgt tgagcatgtt cggccatggc tcgcatgtac
     8041 acgattgtct tcgttagggc cttgccgata tcgccaagtt cgtcgctgcg gttgcgcaac
     8101 ttgtccttgg tgcgctcatc catgtctctg ttgaggtcgc ccacggccag acgttccagg
     8161 acgaggtgta tggttgccac ggatctggaa aggtcgtgac tgagtgcaat gccggttgtg
     8221 agccccacca ggatgaccac ggccaggagc ccgattacga tcactctcgt ctgtgccgct
     8281 tcctggtcgc cgaggtcgtc ctccttagac gcagtgtcag catagtgttg cttaagttgc
     8341 tcggccacct tctccacttt gcccacggag accgccgctt cgtccagcct gccgattgcg
     8401 gtatcgacct gcccctgaat ggctgtcttc gcgatatcat cagccacgtt cttatagtcg
     8461 acgtaggccg actcgaacga cgcgagcaac tgcttctctt gctcagtcgg cgcagtgtct
     8521 ttgtattttg ccagtgactt cgagatctgc tgatcggcgg ccgctatcga gttcagcgcc
     8581 gcagaccgct tggtctcatc cttggcgaaa atgatcacgt tgcgaaggtc tctggcgcga
     8641 gtcatgacgg cgttttgaat gtcacccgtg taggcaaccg gtagcagttg actgtgatac
     8701 gtcgcattga gcatgtcgga gagagagttg gtccgcgtca cagccatggt tcccaggatg
     8761 ccggccagga tcaacaccac gacgtagcct gccaataacc tcgttcggac cgatagcctc
     8821 atgtgaagtt cctttcttgc ctgttggatt gcagcgtgac ctgagagtga cctcctttgc
     8881 atctctctta cggattgaga gtagtctgtt gccagtccac gaatatggtg agatgtgcta
     8941 tccggagaag gtcaagcagg gggcgcatct ctgagagccg tttactggat tacgacggga
     9001 tctcagcgcc aatcgaagct gcaccgtcta aacgggcacc gatgcttgga cctctgccca
     9061 gttgagcact caacctatgc ttagttgttc ggcacggttc gtaaggctgc gtattacccg
     9121 attgggtaat tcatgggaag gggcggtgtc accatcccgc ctgtggagag ggagtagatg
     9181 ccgtatcgac ataagacgga aggggctaac gctcgtggac agtgggagga ggaaaccaga
     9241 ccggccgaac tggggcttac tgcttctcga ccgtcttcct ggtatggttg gggcttagca
     9301 tcgcctgggc aatctctctg ggatcgacct ggtaggtgcc gttggcgatg gcggctctga
     9361 tctcctcgat ccgcgattcg cgaacgtcgg aggacgcgcg gacagcgtcg tgaagcttcg
     9421 atgccagcct ggcgtcatca gtggagatgc tgacctgatc tgcctgggtc gagttgagca
     9481 gacgtttgga cagtctggcg gccgagtccg aatcgggcgg tcgggattgg ctcggaccaa
     9541 tgatccggcc atgaacactc tgatctgaag atatgggatc gctccctgtc acactttcct
     9601 ccagaaacgc cattctcagt catgacgacg agacaacaga ctgtcctatc gatatcaaga
     9661 tatttcaact agcccatcat aacatgtcgt gcactgtttg agattgtacc ctgatctacc
     9721 tgagtgacac aactaccatg tgcatggacg tgcccttgcc gcagtgacct cggattcgcc
     9781 gggttcgtgg gttgccgaca ggtagaggca gttgctattc tgcgtgcagc cttgaattct
     9841 ggtggtgaag agtggataga agcgagaagc gagccttgca cagctccttc tgggagatgg
     9901 ggccgtccga acggcctctg ggatcctgct ggatcggcag ccgtatgccc gacgagatct
     9961 atcgcgctac ccgatcgata ccgattggca aggtaatgcc ttcggatgtc agcgtcgaag
    10021 acttcatgga tgactacgac cgcctgtacc agatgcacga ggctgtggga gacgatgcct
    10081 tctggtcggc atccgcattc tacggtatcc cgtgggtgga ggcgatcctg ggatgtcccg
    10141 tctactactc cggtgacagc ttctgggttg aaccgatctt tcccgagtgg cccgagaggc
    10201 tggacctgcc ccggccggaa gggcaggaat ggctggccaa gctgctggac ttcaccgaag
    10261 cgttggtgag gcatgccgat ggccggtacg cggtcaccgc gacattgatg agggggcctt
    10321 cggacctgat cgccgcggct cgcggccatc tggagatgat ctacgacatg tacgatcacc
    10381 ccgagcagct cgagcggctg gcgatggtgg caaccgatct gtggatccag gttgccagac
    10441 ggcaactgga tctgatcccg cgctacgaag gagggtacgt atcacacttt tatcgcatct
    10501 gggcgccgga gtgggtagtc tgccctcagg aggatgcctc tgcttccttc tcgcccgagt
    10561 tcttcaatcg gatcctgatg cccgcggacg agaagatctc ctccgaattc ccctactcgg
    10621 tgatgcacct ccactctccc acaacctggc ccgtggagca gctgcttgag atagatcgac
    10681 tctcgtgcat cgagatcaac tatgacgaca acgggccgcg gttatccgaa ctaatgcccg
    10741 tgctgcagcg catccaacag gtcaagccgc tgattatcag gggagcgctg actgtgg
//
LOCUS       DATALS010000067         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_185293, whole genome shotgun sequence.
ACCESSION   DATALS010000067 DATALS010000000
VERSION     DATALS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185293"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VHS06_01340"
     CDS             <1..937
                     /locus_tag="VHS06_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX2986804.1"
                     /translation="LKWLSYYENHDHNVSLTCRYFGISRQTFYRWKRRYNPRDLSTLE
                     DRPSSPKKRRGRTWSTQQVEAVRKVREEYPRWGKEKLKVILEGQKIYLSVSMVGRILS
                     YLKGRRVLREPIRAIKTRQRRWKRPYGVRKPKDYPVVAPGDLVQMDTMDVRPEPGAVL
                     KQFTTRDVVSRWDVLELACRATAATAARLLDTILERIPFKVKAFQVDGGSEFMAEFEE
                     ACQARGIRLFVLPPRSPKLNGAVERANRTHTEEFYHWSAASATVGELGPELRAWETIY
                     NTVRPHQALGYLTPKQFLDQWYQQHPREEVLSRTL"
     regulatory      1008..1099
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1221..2066
                     /locus_tag="VHS06_01345"
     CDS             1221..2066
                     /locus_tag="VHS06_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX2986805.1"
                     /translation="MGQLKKRLDLLLVERGLATTREQARALIMAGSTRVDGEMFDKPG
                     TKVAEEAAITVAAPPKYVGRGGLKLEKALDAFQLSVAGKVAMDVGASTGGFTDCMLQR
                     GAGRVYAVDVGYGQLDWKLRSDPRVVVMERTNARYLTSLPEPIDLATMDVSFISVRLV
                     LPAIGRLLAPGGQIVILVKPQFEAGRGQVGKGGVVRDPAVHRAVLEELVSWGDREGFG
                     FQRLAVSPVRGPAGNVEFLALIEPGRTAAEDIGQQVDMVLADAAGLSQSGHHTGEDES
                     QQSSE"
     gene            2100..2966
                     /locus_tag="VHS06_01350"
     CDS             2100..2966
                     /locus_tag="VHS06_01350"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX2986806.1"
                     /translation="MQSVVLVYQPMLPDAKRLAERCCRGVQEAGFHPRLVSAWELSAA
                     VDSTDLRLAVTFGGDGTIIRTARWLAGTGVPIVGVAMGKLGFLTELPPESACDKLPSI
                     LAGNYWVDERLMLSAVVNPSSKRSNGGDADPGVGDSGSSPLIALNDVVIARGASPRVM
                     QIDVAVDGAPLVSYVADGLILASPTGSTAYALSAGGPVLAPGVQGILMVPVAAHLAVL
                     KSLVVPLTSKLEVRASADQPALLVLDGQQQIPLRNGQPVTVGVAQERTIFARTGNPTR
                     FYETLMQSLKKR"
     gene            2976..>3179
                     /locus_tag="VHS06_01355"
     CDS             2976..>3179
                     /locus_tag="VHS06_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2986807.1"
                     /translation="MLSELYIKNFAIIDELRVGFGPGLNVLTGETGAGKSIIVDSLGA
                     ALGQRVGSEVVRTGAPQALAEAFR"
BASE COUNT          648 a          845 c         1100 g          586 t
ORIGIN      
        1 gctgaagtgg ttgtcctact acgagaatca cgatcacaac gtcagcctga cctgtcgtta
       61 cttcgggatc agccggcaga ccttctaccg ttggaagcgt cgatacaacc cgagggatct
      121 atccaccctg gaggatcgtc caagcagtcc taagaagcgg cggggacgga cttggtcgac
      181 acaacaggtg gaagccgtca ggaaggtgcg agaggagtat ccacgctggg gcaaagagaa
      241 gctcaaggtg atactggaag gacagaagat ctacctgtca gtgtcgatgg tgggccgcat
      301 cctgtcctac ttgaagggac ggagggtgct gcgggaaccg atacgagcaa tcaagacccg
      361 gcagcggcgt tggaagcgtc cgtacggcgt acgcaagccg aaggactacc cggtcgtggc
      421 cccaggagac ctggtccaga tggacaccat ggacgtgcga ccggagccgg gggcagtgct
      481 caaacagttc accacccgag atgtcgtgag tcgctgggac gtgctggaac tggcttgtcg
      541 tgccacggca gccaccgctg caagactgct ggacaccatc ctggagcgga taccctttaa
      601 ggtgaaggca ttccaggtgg atggaggcag cgagttcatg gctgagttcg aggaggcgtg
      661 ccaggccaga gggatccgac tcttcgttct gcctcccagg agtcccaagc tgaacggggc
      721 cgtggagagg gccaacagga cccacaccga ggagttctat cattggtcgg cagcctcggc
      781 aacggtgggg gagctcggac cggagcttcg agcctgggag acgatctaca acaccgtcag
      841 accccaccag gctctcggat acctgacacc caagcagttc cttgaccagt ggtaccaaca
      901 acacccgaga gaggaggtgt tgtcacggac gttgtgaacc catacacggg gttgacgccc
      961 ggctgtagga taggtaacct atagatgaat agttgaatag agcctgtatg agggttgctg
     1021 gagagaccgc aaaggggcgg cgccgaaggg gcaagacgac aagctggaat ctctcaggca
     1081 gaaggacagc aacctgacag gatcctggag aggctctctc gaaaagggca ccgaagggga
     1141 tgaacccgcc gcgcgacggg ccgtatctct caggtagatg ggacaggagg catgatgtat
     1201 tcctgtcccc ttttgtgttt gtgggacagt tgaagaaacg tctggatttg ctgctggtag
     1261 agcggggcct ggcaacaact cgggaacagg ccagggccct cataatggcc ggctccacac
     1321 gagtggacgg cgagatgttc gacaagccgg gaaccaaggt ggcggaggag gctgccatca
     1381 ccgttgcggc gccgcccaag tacgtcggcc gtggtggact gaagctggag aaggcgctgg
     1441 acgcctttca gttgtccgtc gccggaaagg tggccatgga cgtgggggcc tccaccggag
     1501 gcttcacaga ctgcatgctg cagcggggcg ctggaagggt ctatgccgta gatgtcggct
     1561 acgggcagct ggattggaag cttcggtctg accccagagt ggtggtcatg gagcgcacca
     1621 acgcaaggta tcttacgagc ctgcctgagc cgatcgatct ggcgaccatg gacgtctcct
     1681 tcatatccgt tcgactcgtt cttccggcga ttggcaggct gctggcgccg ggcgggcaga
     1741 tcgtaatcct tgtgaaaccg cagttcgaag ccggacgagg acaggttggg aagggcgggg
     1801 tcgtcaggga cccagcagtt cacagggcgg tgctggagga attggtatcc tggggagatc
     1861 gggagggttt cggattccag agactggccg tatctcctgt gagagggccg gctggaaatg
     1921 tggagtttct tgccctgatt gaaccgggga gaactgctgc agaggatatc ggtcagcaag
     1981 tcgacatggt gttggcggat gcggcaggat tgtcgcagtc cggccatcat acgggggaag
     2041 acgagtcgca gcaatcgtca gagtagggga atgtggcgtt gcccgtggag gtaagaggcg
     2101 tgcagtctgt ggtgcttgtg tatcagccga tgctccccga tgccaagcgg ttggcggagc
     2161 ggtgctgcag gggtgtccag gaggccgggt tccatcctcg gctggtctcc gcgtgggagt
     2221 tgagtgcagc ggtagacagc accgacctga ggctggccgt aacctttggc ggtgacggca
     2281 ctatcatcag gaccgcacgt tggctggcag gtacgggcgt tcccattgtg ggagtggcaa
     2341 tggggaagct cggcttcctc accgagcttc ctccggaatc tgcctgcgac aagttgccct
     2401 ccatcctcgc cggaaactac tgggtcgatg agcggctcat gctttcggcc gtggtaaacc
     2461 ccagcagcaa gcgcagcaac ggtggggacg cggatccagg ggtgggggat tccggcagtt
     2521 ctcccttgat cgcactgaac gacgtggtga tcgcgcgagg tgccagcccc agggtgatgc
     2581 agatcgacgt ggcggtggac ggagccccgc tcgtgagcta cgtggccgat gggctgatcc
     2641 tggcctcgcc gaccgggtct acggcctacg cactgtcggc gggaggaccc gtgctggcgc
     2701 cgggggttca gggaatactg atggtgccgg tagccgccca tctggccgtg ctcaagtcgt
     2761 tggtagtgcc actgacctcg aagctagagg tgcgagcctc tgctgatcag cctgcgctgc
     2821 tggtactgga cggccagcag cagatcccgt tgcgaaatgg acagccggta accgtgggtg
     2881 tagcccagga gcggaccatc ttcgctcgaa ctggcaaccc aacacggttc tacgaaacgc
     2941 tcatgcagag cctcaagaag aggtagcgat ccccagtgct ttcagaactc tacataaaga
     3001 acttcgccat aatcgacgag ctcagggttg gcttcggtcc cggtctgaat gtcctcactg
     3061 gggagaccgg cgccgggaag tcgataatcg tggattcgtt gggcgcggcg ctgggccagc
     3121 gtgtcggatc ggaagtggtg cgcactgggg cccctcaggc gcttgcggaa gcgtttcgt
//
LOCUS       DATALS010000068         6231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_230960, whole genome shotgun sequence.
ACCESSION   DATALS010000068 DATALS010000000
VERSION     DATALS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230960"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            762..1994
                     /locus_tag="VHS06_01360"
     CDS             762..1994
                     /locus_tag="VHS06_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2986808.1"
                     /translation="MSGKTRITMLIAGTVVALTMAVACSPAAAPAPTAAPAKPATEAT
                     KAPAAPAASTSAPAAAAPSGGTIKIGFTAPLTGDQALFGKTFLNGVMVAVNETNAAGG
                     VLGKKIEVTSLDDQADPKQGPVVAQRLADDKSIVGVIGPFNSGVAIPAAPVYNKARLV
                     NISMATNPKVTTLGFDTFFRTIANDNIQGGTPAEYFTKKLNAKKAAIVHDKQAFGQGV
                     SDVFEKQFKALGGTVTSVNGITATDTDFSAVVTKIKGENPDVIQYGGTHVAGGLLLKQ
                     LRAQGLKQPFMAPDGCYTPEFIQVAGDAAEGAMVTFQIPPYDTTPATQKFAADYKKTF
                     NEDPGPYSGSGYDMAAALIAGIKGAGSDNRDAIVKATHAVKMNGLYGPIEFDATGEVK
                     QPAIFVFQVKNGKFELVK"
     gene            2096..3055
                     /locus_tag="VHS06_01365"
     CDS             2096..3055
                     /locus_tag="VHS06_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2986809.1"
                     /translation="MDTILQQLINGVTVGSMYALIALGYTMVYGVLLLLNFAHGDLFM
                     VGSYVAIAILALAGVTGGLGVAAIAGLLLTIFLVSGFLVAGLGVAIEKVAYKPLRNSS
                     RLAPMLSALGVSLVLENAAMLVAGRAPRVFPNGFFPSQSYHLFGATFTNHQVLILVVS
                     AALLAGMYYLVNRTILGLQIRAVAENRNTASLMGVNIDRTISLVFVIGPALGAAAGVM
                     FSMYYGVAFFTMGFTAGIKAFTAAVLGGIGNIPGAMVGGILLGLLETFGAGLLPIISG
                     GLLTPEYKDIFAFLVLILVLIFRPMGLLGERIPMESMTWKRSF"
     gene            3081..4100
                     /locus_tag="VHS06_01370"
     CDS             3081..4100
                     /locus_tag="VHS06_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2986810.1"
                     /translation="MKLLRRFGPLGLLIVLALAPFPVGDAAAIIRVLDLIAIYCLLAL
                     GLNVVTGFAGLLVLGYAAFYAVGAYTYAFLASAQFNIHWPFLLLLVLGGILAAFFGLL
                     LGIPVLRLRGDYLAIVTLGFGEIIRLFLNNLSTLTNGPQGISLIDTPDLFGYQLSTPT
                     HVYYLLLVLLAVSVVFLRRLEGSRIGRAWAAIREDEGAARAMGVDTTRLKLMAFAIGA
                     STAGLAGVMFAALQGFVSPESFTFWESILMLSMVVVGGIGSIPGVIIGAIALIGIPEL
                     LREYSDYRMLAFGLVMILMMILRPGGIWPSAMVSAEMRAGHGDDTAEETSSAELATPT
                     VGGGN"
     gene            4100..4924
                     /locus_tag="VHS06_01375"
     CDS             4100..4924
                     /locus_tag="VHS06_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2986811.1"
                     /translation="MAPILEIRELSKQFGELRAVDKVTLTVQEGQIFAIIGPNGAGKT
                     ALFNLITNTYHATSGEVVIAGKQTLKLPEHKLTELGVARTYQNIRLFDNMTALDNVKV
                     GMHCRTKSGVLGAILALPGTKREEKAGDARAMELMEFVGVTRYARDMAKNLPYGAQRR
                     LEIARALATTPKLLLLDEPGAGMNPQETGDLLELIKKIQGIGVTVLLIEHHMKVVMGV
                     SEHIVVLNYGAKIAEGTPEEIQNNQLVIEAYLGTKRETGFARSRRHRHEVRTDSGA"
     gene            4872..5579
                     /locus_tag="VHS06_01380"
     CDS             4872..5579
                     /locus_tag="VHS06_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2986812.1"
                     /translation="MLVVEDIATKYGQIAALKGISFRVETGEIVALIGANGAGKSTTL
                     RTLSGVLHPWKGKVIFEGKEIQGRRPWDITRMGIGQCPEGRQIFTRMTVEENLRIGHY
                     TQRHNTKFEEEADRVTARFPRLKERWKQVGQTLSGGEQQMLAIARVMMSRPRLLLLDE
                     PSLGLAPIIVERIFETIDEINARGTTILLVEQNAQLALAMADRAYVLETGRIVLEGTG
                     EELLENPAVRKAYLGIS"
     gene            complement(5734..6156)
                     /locus_tag="VHS06_01385"
                     /pseudo
     CDS             complement(5734..6156)
                     /locus_tag="VHS06_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT         1123 a         1861 c         2043 g         1204 t
ORIGIN      
        1 cggatgacga ctgattaccc tcgtgacctg gaaagtctca tcttctgagg accgagtgga
       61 tttcctgaat ccaatcctga tcaaaatgtg cgtcggggtg aacgggcttc ccccgacgca
      121 ctttccttca tgctttgtat ggttccattt gggcaaagtg ttcagtcgca accccatcat
      181 ttgccattga tttctggtat tctgcacaaa gacagggtaa agccctgccc ctaaaatggc
      241 cgtacgcgga ctgtctgtag acagcagcag tccatccact gcgtgtttct ttcctgcatg
      301 tcctcgatcc acgtcctctg ttgtgttttg gcttggcccc ttatggtgga gttgctgaca
      361 tcgtctggga ccgagcaagg atgtcgagga tggattgccg accagatgcc gccggaggct
      421 ctccgtgtaa ggcgattgga ttgacaaggg cggcaccgag ttttcataat gcgtacgcct
      481 cgtcggcgat tggtcgccga tcagcggttg cctcctcgac cccggccgcg gggcggtcgc
      541 ttccaacggt ttcctcaggc ttaaagcgct tgaaggctgg tttcggttgg gacccatttg
      601 gctggccggg gtgttgggtc agtctggaga ggaggtggag ggccgagagt aatctggtgc
      661 tttggtggcg ctgagccgcg cccgcgcggg cgtggagcgc cggtgggcct gcgcgcacag
      721 cgtggtgcgg gcttccgaga tgagatttgg aggatgaaca gatgtcgggc aagactagga
      781 tcacgatgct catagccggt acggtggtgg ctctaaccat ggccgtcgcg tgctcacccg
      841 cggctgcgcc tgcccccacc gcggcgcccg ccaagcccgc taccgaggcg accaaagctc
      901 cggctgctcc cgcggcctct acctcagcgc ctgccgcagc ggcaccgagc ggcggaacca
      961 tcaagatcgg tttcaccgcg cccctcaccg gagaccaggc tctgttcggc aagacctttc
     1021 tgaacggtgt catggttgcg gtcaacgaga ctaatgccgc cggcggcgtg ctgggcaaga
     1081 agatcgaggt gacctccctg gacgaccagg ccgatccgaa gcagggcccg gtggtggccc
     1141 agcgcctagc cgacgacaag tccatcgtcg gcgtaatcgg tcccttcaac agcggcgtcg
     1201 ccatccccgc ggcccccgtg tacaacaagg cgcggttagt gaacatctcc atggctacca
     1261 accccaaggt caccacgctg gggttcgaca ccttcttccg caccatcgcg aacgataaca
     1321 ttcagggcgg tacgccggcc gagtacttca cgaagaagtt gaacgccaag aaggccgcca
     1381 tcgtccacga caagcaggca ttcggccaag gtgtttcaga cgtcttcgag aagcagttca
     1441 aggcgttggg cggcaccgtc accagcgtca acggcatcac cgccaccgac accgacttct
     1501 ccgcggtggt caccaagatc aagggcgaga acccggacgt catccagtat ggtggcaccc
     1561 acgttgcggg cggtctgctg ctgaagcagt tgcgggctca ggggctcaag cagcccttca
     1621 tggcgcctga cggctgctac accccggagt tcatccaggt agccggcgac gccgccgagg
     1681 gggcgatggt caccttccag atcccgccct acgatacgac gcccgcgacc cagaagttcg
     1741 cggccgacta caagaagacc ttcaacgagg atcccggacc ctactccggc agcggctatg
     1801 acatggctgc agcgctgatc gctgggatca agggtgcggg ctccgacaac cgcgatgcga
     1861 tcgtcaaggc cacccacgcg gtcaagatga atggcctgta cgggccgatc gagttcgatg
     1921 ccacgggcga agtcaagcag cctgcgatct tcgtcttcca ggtgaagaac ggcaagttcg
     1981 agctagtcaa gtaaccaggc ccaagggccg ggtattgggg gttgggggat aggggttgga
     2041 gagacggtgc cgacccccaa cccccatccc ctatccccca aagtgaggac tgccagtgga
     2101 tacaattctc cagcagctta tcaacggtgt caccgtagga tcgatgtacg ccctgattgc
     2161 cctgggctac acgatggtct acggtgtttt gcttcttctg aactttgctc atggggacct
     2221 cttcatggtg gggtcctacg ttgccattgc gatattggcc cttgccgggg tgaccggtgg
     2281 gctgggagtg gcggcgatag ccgggctgct gctaaccatc ttcctggtta gcggattcct
     2341 tgtagccggc ctcggcgtgg ctatcgagaa ggtggcctac aagccgctgc gcaactcctc
     2401 ccgactggcg cccatgctga gtgccctagg cgtctccctc gttctggaga acgctgcaat
     2461 gctggtggcc ggtcgagcgc cccgggtctt ccccaacggc ttcttccctt cccagagcta
     2521 ccaccttttc ggtgcgacat tcaccaacca ccaagtgctc atccttgtcg tttccgcggc
     2581 gttgctggcc gggatgtact atctggtgaa ccgcaccatc ctggggctcc agatccgcgc
     2641 cgtggctgag aaccgaaaca ccgccagcct gatgggggtg aatatcgacc ggaccatctc
     2701 cctggtgttc gtcatcggtc cggctctggg tgctgccgcg ggcgtcatgt tttccatgta
     2761 ctacggcgtg gctttcttca ccatgggctt caccgccggc atcaaggcgt tcaccgccgc
     2821 ggtgctcggc gggatcggca atatccccgg cgccatggtg ggcgggatcc tgttggggtt
     2881 gctggagacc tttggcgccg gcctgttgcc gataatctca ggagggcttc tgacccccga
     2941 gtacaaggat atcttcgcct tccttgtgct gatcctggtg ttgatcttcc gtcccatggg
     3001 gctgcttggc gagcggatcc cgatggagtc catgacctgg aagcgaagct tctagtcacc
     3061 cttaaggagg taggagagca atgaagttgc tccggcgctt cggccctttg gggctgctga
     3121 tagtcctggc gctggctccc ttcccggtgg gtgacgccgc tgctatcatc cgagttctgg
     3181 acctgatcgc gatctactgt ctgctggccc tggggctgaa cgtggtcacc ggctttgccg
     3241 gcctgctggt gctggggtac gccgccttct acgcggttgg cgcctacacc tacgcatttc
     3301 tggcctctgc ccagttcaac atccactggc cattcctgtt gctgttggtt ctgggcggca
     3361 tcctggccgc attctttggt ctgctgctgg gcatcccggt actgcggctg cgaggtgact
     3421 acctggcgat tgtcaccctc ggcttcgggg agatcatccg gctcttcctg aacaacttga
     3481 gtacgctgac caacggaccg cagggtatct ctcttatcga cacgcccgat ctgttcgggt
     3541 accaactctc tacccccact cacgtttact atctgctgct ggtgctgctg gcggtcagtg
     3601 tggtgttcct gcggcggctg gagggttcgc ggataggcag ggcctgggcg gcgatccgag
     3661 aggacgaggg ggcggctcga gccatgggtg tggacaccac ccgactgaag ctgatggcct
     3721 tcgctatcgg cgcctcgacc gcggggcttg caggtgtgat gttcgccgcg ctgcagggct
     3781 tcgtgagccc cgagagcttc accttctggg agtcgatcct gatgctctcc atggtggtgg
     3841 tagggggcat cgggagcatc cccggcgtga tcatcggggc gatagcactg atcggcatac
     3901 ctgagctgct gagggagtac tccgactacc ggatgctggc cttcggcctg gtgatgatcc
     3961 tgatgatgat cctccgcccc ggcgggatct ggccgagcgc catggtcagc gccgagatgc
     4021 gcgccggcca tggggacgat accgctgagg agacgtcgtc cgccgaactg gcaactccca
     4081 cggtgggagg tggtaactga tggcgccgat cctagaaatc cgagagctgt cgaagcagtt
     4141 cggcgagttg agggcggtgg acaaggtgac gctgacggtg caggagggcc agatcttcgc
     4201 gatcatcggc cccaacggcg ccggcaagac cgccctgttc aacctgataa ccaacaccta
     4261 ccacgccacc agcggcgagg tggtgatcgc gggcaagcag acgctgaagc tgcccgagca
     4321 caagttgacc gagctgggcg ttgccaggac ctaccagaac atccggctgt tcgacaacat
     4381 gacggcgctg gacaacgtga aggtggggat gcactgccga acgaagtccg gggtgctcgg
     4441 ggccatcctg gcgctacccg ggaccaagcg ggaggagaag gccggcgacg cccgcgcgat
     4501 ggagttgatg gagttcgtgg gtgtgacccg ctacgcccgg gacatggcca agaacctccc
     4561 ctacggggcg cagcggcggc tggagatagc gcgcgcgctg gcgacgacgc cgaagctgct
     4621 gctgctggac gagcccggcg cggggatgaa cccgcaggaa accggggacc tgcttgagct
     4681 gataaagaag atacagggta tcggggtcac ggtgctgctg atagagcacc acatgaaggt
     4741 ggtgatgggg gtatcggagc acatcgtggt cctgaactat ggtgcgaaga tcgccgaggg
     4801 gacgccggag gagatccaga acaaccagct ggtgatcgag gcgtatctgg gcacgaaaag
     4861 ggagaccggc tttgctcgta gtcgaagaca tcgccacgaa gtacggacag atagcggcgc
     4921 ttaaggggat ttccttccgg gtagagaccg gggagatcgt ggcgctgata ggtgcgaacg
     4981 gggcgggtaa gagcacgacg ctgcggacgc tctctggagt gctgcacccc tggaagggga
     5041 aggtgatctt cgaggggaag gagatccagg gacggcggcc atgggatatc actcggatgg
     5101 ggatcggcca gtgcccggag ggtcggcaga tctttacccg gatgaccgtg gaggagaacc
     5161 tccgaatcgg acactacacg cagcggcaca acacgaagtt cgaggaagag gcggatcgtg
     5221 tgacggcgcg gttcccgcgg ctgaaggagc ggtggaaaca ggtagggcag acactgtctg
     5281 gcggcgagca gcagatgctg gccatcgcga gggtgatgat gagccggccg aggctgctgc
     5341 tgctggatga gccctccctc ggattggcac cgatcatcgt cgagcggatc ttcgagacga
     5401 tcgacgagat caacgcccgg ggcacgacga tcctgctggt ggagcagaac gcgcagctcg
     5461 cgctggcgat ggcggatcga gcgtacgtgc tggaaaccgg aaggatagtg ctggagggca
     5521 ccggggagga gttgctggag aacccggcgg tgcggaaggc atacctgggg ataagctgac
     5581 gtaatggaac tcggaggaac tccggggaac tcatagggat ttggaccgca aaggcacgaa
     5641 ggtgttctct tcgtgccttt gcggttctgt gcataatgtt ctgtcacggg cttccagcgg
     5701 atggtagggg ccgtgacgct gtgctcgctc gcgtcacgct gcgtgtgcag ggaggattcg
     5761 atatggaacg gctatgggac gatcgtacgc gcccgtggcc accacgatag caccggcccg
     5821 aagtgactcc accccctctc cctcgcgata gaggagcagg gtcttgccct cttcgacccc
     5881 ccagacgaaa gtttcgggga gcagttccac cgaggagccg gacaggctat ggaacagctc
     5941 tagggccttc accttctccc tgctgttggg gcccggcccg atgggccgga agggttcggc
     6001 agcctgccgg tagatctggc cgcccggccg cgggtactcg tcgacgacgg tgacctgtgc
     6061 gcccgctcga tcggctgcca gtgcagcggt aagccccgcg gggcctgcgc ccaccacggc
     6121 gatttcagtc tgccgcactt ccggcctccc ctccgcagtc gccctgccct cgcaggctca
     6181 ccttcatgcc ggggcgaacc cccaccatgc agctgcgaac acgccccatc c
//
LOCUS       DATALS010000069         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_5996,
            whole genome shotgun sequence.
ACCESSION   DATALS010000069 DATALS010000000
VERSION     DATALS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5996"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VHS06_01390"
     CDS             complement(<1..429)
                     /locus_tag="VHS06_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX2986813.1"
                     /translation="MLPSRWISYSIVQLVLLLLVSAMGTTPTSAEGPYAAVVPFKGIV
                     NPAMATYVDRAIAQAEESGASVIIIEMDTPGGLDTSMRDIIQRIIASRVPVVVYVAPN
                     GSRAASAGVYITYAAHIAALAPSTNIGSAHPVALGDNGSEA"
     gene            complement(532..1155)
                     /locus_tag="VHS06_01395"
     CDS             complement(532..1155)
                     /locus_tag="VHS06_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986814.1"
                     /translation="MTRLFSPGLAQIKQHWSAYVILNICFYGLILAGSTYAFLRPDIQ
                     RQLMASVIQGFSSGLLAYARDAYLSRDVAAAAAITFGVNTFIGSFATLTLPSLFIPFF
                     GSFLGLLRGLVLGVTLAPSSPELARAMVPHLPVIIVEGQGYVLAMLGVHILWTSAFSA
                     PGGGISGFVRGYRAGLRANLAIYALIVAVLAVAAVYEAIELIYIVAR"
     gene            complement(1148..1855)
                     /locus_tag="VHS06_01400"
     CDS             complement(1148..1855)
                     /locus_tag="VHS06_01400"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2986815.1"
                     /translation="MGNVRRRFKAVLFDLYDTLIWLDSERSANWRRQLAAAAGVDLDR
                     FSVAWRRTVNDRMLGKGSGLAGHLSEALSELGVPHDAELVAKLVSIERRRLEECVHLY
                     PSTIPVLRELSLRGYKLGLISNISDGASVPITYLGLDLCFDDMILSHEVGILKPDPAI
                     FVLACDRFDVAPAKTIFVADGGFGELDASRGLGIYSVLVEQEHQSRDYGASQEYDLKI
                     HDLAELLAILPGADRDD"
     gene            1981..>2166
                     /locus_tag="VHS06_01405"
     CDS             1981..>2166
                     /locus_tag="VHS06_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2986816.1"
                     /translation="MDLGLKGKVVLITGGSHGIGKAIALRVAAEGASVAICARTEGHL
                     RETASEIAEKTGADVLPV"
BASE COUNT          459 a          630 c          709 g          368 t
ORIGIN      
        1 cgcctcggaa ccgttgtccc ccagggcgac cgggtgcgca gagccgatgt tggtgctagg
       61 cgccagggca gcgatgtggg ctgcgtaggt gatgtagacg cccgccgagg ccgctcggga
      121 accattgggt gcgacgtaga cgacgaccgg cacgcgggat gcgatgattc gctggatgat
      181 gtcgcgcatg gaggtgtcga ggccgccggg cgtatccatc tcgatgatga tcactgatgc
      241 tccgctctct tcggcctggg caatcgcgcg atcgacgtaa gtagccatcg cggggttcac
      301 gatgcccttg aatgggacga cggcagcgta cggaccctcc gcggacgtcg gcgttgtacc
      361 catcgcgctc accaggagca gcagcactaa ctggacgatc gaatacgaga tccatctcga
      421 aggcagcatc ttggtccacc tcctttgatg agcatgttag cacgtcggcg gtcaagctgt
      481 tagctcttag cgcttggcaa ctggctgttg accatgatcc caaaccatgc cctaccgggc
      541 cacgatgtag atcagttcga tggcctcgta cacggcagcg acggcaagga cggccactat
      601 gagggcgtag atggcgaggt tcgcacggag gccagcgcga tagccccgca cgaagccgga
      661 gatgcctccg cccggagcac tgaacgcgga ggtccacagg atatgcacac ccagcatcgc
      721 gagaacgtag ccctgcccct cgacgatgat caccggtagg tgaggcacca tcgcccgggc
      781 aagctccggg gaggagggcg ccagcgtgac gcccaacacc aggccgcgaa gcaggcccaa
      841 gaagctgccg aagaagggga tgaatagcga tggcagggtc agagtcgcaa acgatcctat
      901 gaacgtgttg actccgaagg ttatggcagc tgccgccgcc acgtcccggc tcaggtaggc
      961 atcacgtgca taggcaagca gacccgaact gaagccctgg attacggagg ccatcaactg
     1021 tcgctggatg tccggccgca gaaaggcgta ggtagaaccg gcgagaatga ggccgtagaa
     1081 gcagatattg aggatgacgt acgccgacca gtgctgcttg atctgggcaa gacccgggga
     1141 gaacagccta gtcatcgcga tctgcgccag gaaggatcgc caacaactcg gccaggtcgt
     1201 ggatcttcag gtcgtattcc tggctggcgc cgtaatccct gctctggtgc tcctgttcga
     1261 ccaggacgct gtagatcccg agcccgcgcg acgcgtccag ttcaccaaat cccccgtccg
     1321 cgacgaagat cgtcttggca ggagccacgt caaatcggtc acaggccaga acgaagatgg
     1381 cggggtcggg cttaagtatg cccacttcgt gggataggat catgtcatcg aaacagaggt
     1441 ccaggcccag ataggtgatt ggcaccgagg ctccatctga gatgttgctg atcaggccca
     1501 gtttgtagcc tctaagtgag agctcgcgta gaacgggaat cgtgctcggg tacaggtgga
     1561 cgcactcctc caaccgtcgt cgctctatgg agactagctt ggccaccagc tccgcatcgt
     1621 gtggaacgcc cagttcggaa agggcctccg acaggtggcc cgcgaggccg ctgcctttcc
     1681 ccagcatccg gtcgttcacc gtgcggcgcc aggcgacgct gaaccggtcg aggtcaacgc
     1741 ccgcagcggc ggcgagttgg cgccgccagt tggcagaccg ctcggagtcc agccagatca
     1801 gagtgtcgta cagatcgaag aggacagctt tgaaacgacg gcgcacgttt cccatttcgg
     1861 gactgactat acagcctgga gggtggctgc tacaagaaaa cgaattcccg ttgccacctg
     1921 ccttcctggg gcgtatagtc caaacacgag accggttgtt gacagcaagc gaggaagaag
     1981 atggatctgg gcttgaaggg caaagtcgta ctgatcaccg gcggaagcca cggcatcggg
     2041 aaggcgattg cgctccgggt cgccgctgag ggcgcgagtg tggccatatg cgcgcggacg
     2101 gaagggcacc tccgggagac agcctcggag atcgcggaga agactggggc tgacgtgtta
     2161 cccgtg
//
LOCUS       DATALS010000070         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_140395, whole genome shotgun sequence.
ACCESSION   DATALS010000070 DATALS010000000
VERSION     DATALS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140395"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VHS06_01410"
     CDS             <1..686
                     /locus_tag="VHS06_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2986817.1"
                     /translation="QGIGRAAALALAGAGCAVALAARTASDLDRVAEEIRAMGGRALA
                     VPTDVANEGEVETLVRRTLAEIGPIDILVNDAGTNTRKPIWEVTTEDWQRILGVNTTG
                     TFFCTRAVLPTMMERRSGKIINVSSMAGKIASPTRSAYSAAKFGVVGFGQAIQRELNE
                     YGITISTVFPGPIATAMRRKNNPGEDPSGLVKPEEVGEAILFLATRSSNAVIPELAIY
                     PRSFIAGAE"
     gene            complement(738..1775)
                     /locus_tag="VHS06_01415"
     CDS             complement(738..1775)
                     /locus_tag="VHS06_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX2986818.1"
                     /translation="MNYDLRGYRFSREEFFPKAIFDRIADVRVNRPEAVRDYAARRRR
                     RRSLTRDGKLVILASDHPGRMVTAMLGDPLGMGNRVEYLGRILRVAATVKLDGMMSTP
                     DFIEDLMIVDMLNVEAGGPSFLDEQLLIGCVNRGGLIGAKFEMDDRITAYTPKGIAKM
                     GLDGAKLMFRIDLADEGSGKTLEYCARTIDECNDLGLPVFLEPLPSEKVDGVYRVKRV
                     MADNIRQMGVASALGYSSLNTWLKIQFTPDFDRVVQASSLPLLMLGGESKGNPTEMLE
                     QFQTGMSLGGNVRGAMVGRNVTYPGPDDPAGVAAAVNAIVHEGAGLEDAYAALARMRG
                     TQMDVLKRLML"
     gene            complement(1876..>2477)
                     /locus_tag="VHS06_01420"
     CDS             complement(1876..>2477)
                     /locus_tag="VHS06_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2986819.1"
                     /translation="QALTSLMADGLLYRQRGLGTFVRKSRIEQELSSLTGFSEEMVSR
                     GLVPGTRLVSAEMVEADGTVAPRLSIAPGQIVFRMVRVRLADGEPTALDVTCLPSDIG
                     ERLLKEDLEEALYTHFARMGVELDWGDQAIQAAPADDLMAHHLQVKKGTPVLLMERVV
                     STTDGRVVEFSRTAYRSDRYIYRVRLKRGKSAASAGAQH"
BASE COUNT          461 a          811 c          770 g          435 t
ORIGIN      
        1 gacaggggat cgggcgggcg gcggccctgg cacttgcagg agcaggctgc gcagtggccc
       61 tggctgccag aacggcctca gacctcgacc gggttgcaga ggagatccgc gcgatgggag
      121 gaagggccct cgccgtccca accgacgtgg cgaacgaggg tgaggtcgaa acgctcgtcc
      181 ggcggacact cgccgagatc ggtccaatcg acatactcgt caacgacgcc gggacgaaca
      241 cgcgaaagcc gatctgggag gtgaccaccg aggattggca gaggatcctc ggcgtcaaca
      301 cgacggggac attcttctgt actcgagcag tgttgcccac gatgatggaa cggcgctccg
      361 ggaaaatcat aaacgtgtca tcgatggctg ggaaaatcgc ctcgccgacg cgctcggctt
      421 actcggcggc caagttcggc gtcgtgggct ttggccaggc gatccagcgc gagttgaacg
      481 agtacgggat cacgatcagc accgtgttcc ccggccctat cgccactgcc atgcgccgaa
      541 agaacaatcc tggagaggat ccgtcagggc ttgtcaaacc ggaggaagtc ggggaggcga
      601 tcctgttcct ggcgaccagg tcctcgaatg cggtcatccc cgagcttgcc atctatccga
      661 ggtcgttcat cgcgggagcc gaatagtcga gagacagggc cgggcgatcg atgcaatcgg
      721 cacccggccc ctccacccta cagcatcagt cgcttcagca catccatctg cgtgcctcgc
      781 atacgggcca gcgcagcgta ggcatcttcg aggccggctc cctcgtggac gatggcgttc
      841 acggcggctg ctacgcccgc agggtcgtcc ggacccgggt aggtgacgtt ccgtcccacc
      901 atcgcaccgc gcacgttgcc gcccagcgac atgccggtct ggaactgctc cagcatctca
      961 gtagggttcc ctttggattc gccgcccagc atcaggagag gcaggctgga tgcctggacc
     1021 acccgatcga agtccggggt gaactggatc ttgagccagg tgttgagaga ggagtagccg
     1081 agggccgaag caacccccat ctgtctgatg ttgtcggcca tcacccgctt gaccctgtac
     1141 acgccgtcga ccttctcgct cggcagcggc tccaggaaga cgggaagccc cagatcgttg
     1201 cactcgtcga tggtgcgggc gcaatactcc agtgtcttgc cgctgccctc gtcggccaga
     1261 tcgatgcgga acatcagctt cgccccgtcc agccccattt tggcgatgcc cttcggcgta
     1321 taggccgtga tgcgatcgtc catctcgaac ttcgcgccga tcaggccgcc gcggttcacg
     1381 catccgatga gtagctgctc gtcgaggaaa ctggggccgc ccgcctccac gttcagcata
     1441 tccacgatca tcagatcctc gatgaagtcg ggcgtagaca tcatgccgtc caacttcacc
     1501 gttgcggcaa cccggaggat cctgccgagg tattcgacgc gatttcccat gccgaggggg
     1561 tcgcccaaca tggccgtgac cattctgccc ggatggtccg aggccaggat gaccagcttg
     1621 ccatccctcg tcagggaccg tcgtcgacgc cgccgggcgg cgtaatccct cactgcttcc
     1681 ggccggttta cccgaacgtc ggcgattcgg tcgaagatgg ccttggggaa gaactcctcc
     1741 cgagagaacc ggtagccccg aaggtcgtag ttcatcgcag ccctcacaga ccgaaaatgt
     1801 ggcaggccgg ggcacgggcg ccccgcccat cgctcaacat cgtctctccc gattggtcgc
     1861 ttccgctgga ataggtcagt gctgtgcgcc ggcagaagca gcgctcttgc ctcgcttgag
     1921 tcgcacgcgg tagatgtatc gatcggacct gtatgccgtc ctggaaaact cgaccacccg
     1981 gccgtccgtc gtggagacaa ccctctccat caggaggacg ggagtcccct tcttgacctg
     2041 caggtgatgc gccatcaggt cgtcggcggg agccgcctgg atcgcctggt caccccagtc
     2101 caactccacc cccatcctgg caaaatgggt gtagagggcc tcctccaggt cttccttcag
     2161 cagccgctcg ccgatgtccg aggggagaca ggtgacgtcc agggccgtcg gctcgccgtc
     2221 ggcaagcctg accctgacca ttcgaaatac aatctgtccc ggggcgatgg agagcctggg
     2281 ggcaaccgtg ccatcagcct cgaccatctc cgcggagacc agtctggtcc cggggacgag
     2341 accccgggat accatctcct cagaaaagcc ggtgagcgag gagagttcct gctcgatgcg
     2401 gctcttccga acgaaggtgc cgagccccct ctggcgatag aggagcccat ccgccatcag
     2461 gctggtgaga gcctgcc
//
LOCUS       DATALS010000071         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_140526, whole genome shotgun sequence.
ACCESSION   DATALS010000071 DATALS010000000
VERSION     DATALS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140526"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..840)
                     /locus_tag="VHS06_01425"
     CDS             complement(115..840)
                     /locus_tag="VHS06_01425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2986820.1"
                     /translation="MTEDKAIILGHPSYVWRRGQDRRLDLIRKYVPLEGRRILDVGCG
                     LGMYVEKFRRYSEEVYGVDIDPEKVEIAQKRLPNISVAPAEDLPYKDRSFDVILLHEV
                     IEHVVDDRRAIEEALRCIDIGGCVIIYAPNRLYPMETHGFYFRGQYHFRLLPLVNYLP
                     DRLRDYFCPHVRVYTGRGIKRLFEGHQVRFLVSTHIYPGFDNVVSRRPALGRFLQQAT
                     DTLEQTPLRAFGISHFVVAQRLA"
     gene            complement(1009..>1893)
                     /locus_tag="VHS06_01430"
     CDS             complement(1009..>1893)
                     /locus_tag="VHS06_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986821.1"
                     /translation="AFPNGGGSDGLLALINLEIWGLPTSKPTMDPANSNFVYLRFQRG
                     IMHFDRTTGLTQGILLADYMKSLITGQNLPGDLAAEANTSALYLQYNNSYIGGLNRPS
                     ALPGTNMFAAFEKDEVTVAPATAQAPAPATTPAAPALAPAAATPVVGRPDLVTIVGND
                     YFTAQTKKALELLPASIRDCVWKIVEAPAPSYVDLKNRTLYVSEADAFPYDWQSKSEI
                     QQQWYAGEIMHASVHLEQYYTGQPYQGTAAEQAALLRMKAFTSTIDTSDGRKLTVGAT
                     AAVNAGNGGYGHFELRNP"
BASE COUNT          355 a          513 c          630 g          395 t
ORIGIN      
        1 caggaccagg aaagcggtcc tgatgggtga ggacttcggg gcggcacgat agacccgccg
       61 ccgatgccgc gtagtgcgaa cactcatttt ctcttcactc gtgatccgcg acgcttatgc
      121 cagccgttgg gcgacgacga aatgggagat gccgaaggcg cggaggggag tctgctccag
      181 tgtatcggtc gcctgttgca ggaagcgacc cagtgccggg cgcctggaaa ccacattgtc
      241 gaagcccgga taaatgtggg tggaaaccag gaagcgaacc tggtgccctt caaacagccg
      301 cttgatgccc ctgcccgtgt atacccgcac gtgggggcag aagtagtctc gtagcctgtc
      361 gggaaggtag ttcaccagtg gcaagagccg gaaatgatac tggccgcgga aatagaagcc
      421 atgggtctcc attgggtaga gcctgttggg agcatagatg atgacacagc cacctatgtc
      481 gatgcatcgc agcgcctcct cgatggcgcg ccggtcgtcc accacgtgct caataacttc
      541 gtgcagcagg atgacgtcga agctgcgatc cttgtaagga agatcctctg ctggtgccac
      601 ggagatgtta ggaagccgtt tctgggcgat ctcgaccttc tcggggtcga tatcgacgcc
      661 gtagacctcc tcgctgtagc ggcgaaactt ttcgacgtac attccgaggc cgcagcccac
      721 atccaggata cggcggcctt ccagtggaac gtacttgcgg atcagatcga ggcgacggtc
      781 ttgccctcgc ctccacacat agctggggtg acccaggatt attgccttgt cttcagtcaa
      841 tgaaggaatc caatgttgag gttgtcgaaa agggcgggcg attctgaaaa ggactgattc
      901 cttgatgagc cctgttgcat cctagcagat ggcgatagct ctttcaagga tagaggggga
      961 aaggagggag gggcggagcg aaccttcctc ccctccctgg gcgctatgct acgggttgcg
     1021 aagctcgaag tggccgtagc cgccgttgcc ggcgttgacg gcggccgtcg cgccgaccgt
     1081 cagcttcctg ccgtcggaag tgtcgatagt cgacgtaaat gccttcatgc gcagcagcgc
     1141 agcctgctct gctgctgtcc cctgatacgg ttggccggtg tagtactgct ccaggtgcac
     1201 cgaggcgtgc atgatctcgc ctgcatacca ctgttgctgg atctcgctct tgctctgcca
     1261 gtcgtatggg aatgcatccg cctcggagac gtacagcgtt cggttcttaa ggtcgacgta
     1321 ggagggtgcg ggggcttcga cgatcttcca aacgcagtcg cgaatggagg ccggcaacaa
     1381 ctctagggcc ttcttagttt gagccgtgaa gtagtcgttg ccaacgatgg tcaccaggtc
     1441 gggacgcccg acaaccgggg ttgctgctgc cggcgccagt gctggggctg ctggagtcgt
     1501 cgccggggca ggtgcttgag ccgtggcggg tgcaacggtc acctcgtctt tttcgaaggc
     1561 tgcgaacatg ttggtgcccg gtagggcgga gggtcggttg agtccgccga tgtagctgtt
     1621 gttgtactgc aggtagaggg ccgacgtgtt ggcctcggct gccaggtcgc cgggcaggtt
     1681 ctggccggtg atgagcgact tcatgtagtc ggcgagcagg atgccctggg tcaggccggt
     1741 ggtcctatcg aagtgcatga tgccgcgctg gaaacgcaga tagacgaagt tgctgttggc
     1801 ggggtccatc gtgggcttgc tggtgggcag gccccagatc tcaaggttga tcagggccag
     1861 caggccatca cttccaccac cgttggggaa ggc
//
LOCUS       DATALS010000072         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_6134,
            whole genome shotgun sequence.
ACCESSION   DATALS010000072 DATALS010000000
VERSION     DATALS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6134"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VHS06_01435"
     CDS             complement(<1..225)
                     /locus_tag="VHS06_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX2986822.1"
                     /translation="MGGKGAGLASMTKAGLPVPPGFTITTEACNEYYAQGEKFPEGMW
                     EQALEALKDVEKKTGKKLGDPSNPLLVSVRS"
     gene            complement(404..1690)
                     /locus_tag="VHS06_01440"
     CDS             complement(404..1690)
                     /locus_tag="VHS06_01440"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX2986823.1"
                     /translation="MDKIVSLCKRRGFVFPNSEIYGGFGSTWDYGPLGSLLKNNVKSA
                     WTRAVVQERDDMFQIDAAILMAPQVWQASGHLANFTDPLVDCKVCRQRFRADHVEGDR
                     CPSCGGELTEARQFNMMFKTFVGPVEDDASVTYLRPETAQGIFVNFKNVQTTTRKKLP
                     FGIAQVGKAFRNEITPGNFTFRTREFEQMEIEFFVVPGTDTEWHEHWVKERMGWYERF
                     GMRMENLKLRPHDPAELAHYAKATVDIEYQFPFGWSELEGIANRTDFDLRQHQAVSGE
                     DLTYFDEQTHERVLPYVIEPSAGADRATLAFLVDAYDEEQDKDETRVVLRFHKELAPI
                     KVAFLPLSKKPDLAALTKKAQGLVRPLFMTAYDDTQSIGRRYRRQDEIGTPYCVTIDF
                     QSLEDQAVTIRDRDTMEQVRVPIEELPHWFVDAFKR"
     gene            complement(1902..2613)
                     /gene="trmD"
                     /locus_tag="VHS06_01445"
                     /pseudo
     CDS             complement(1902..2613)
                     /gene="trmD"
                     /locus_tag="VHS06_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
     gene            complement(2625..>2965)
                     /locus_tag="VHS06_01450"
     CDS             complement(2625..>2965)
                     /locus_tag="VHS06_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX2986824.1"
                     /translation="VAVELGSVGEEEARLLARSTPSVEVVTVDDVEKALAQVAGGGAD
                     AIIVDRPSIPVGTSTMAELKPVAFPLRKQPYSVAVGRKDPGLLLAINRELEAMRADGT
                     LDRLDARWLR"
BASE COUNT          569 a          993 c          820 g          583 t
ORIGIN      
        1 ggaccgaacc gagacgagca gggggttgct tggatcgccc agcttcttgc cggtcttctt
       61 ctcgacgtcc ttgagggcct cgagcgcctg ctcccacatc ccttcgggga acttctcgcc
      121 ctgggcatag tactcgttgc aggcttcggt cgtgatggtg aagcctgggg gaacaggtag
      181 cccggccttg gtcatggatg ccagaccagc gcctttccct cccaggagtt cgcgccacgt
      241 gccgtcgcct tcttcgaaga ggtaaaccca cttttttgcc ggttgccgac gcggattgcg
      301 acatagagtt ttctcctcct atactgcagc cacccggaag tcagcctgta ctgtggcctc
      361 cgaggccacc acgacggtca gttcctatcg gtggccttgc ttgctagcgt ttgaaagcgt
      421 ctacgaacca gtgaggcagc tcctcaatgg ggacccttac ctgctccatc gtgtccctat
      481 cccggatggt tacggcctga tcctcgaggc tctggaaatc gatggttacg cagtatgggg
      541 taccgatctc atcctggcgg cgataccgcc ggccgatgct ctgagtgtcg tcgtaggcag
      601 tcatgaagag tggacgcacc aacccctgtg ccttcttagt gagggccgcc aggtctggct
      661 tcttcgacag cggcaggaac gccaccttga tcggtgccaa ctccttgtgg aagcggagaa
      721 cgacgcgggt ctcatcctta tcctgttcct cgtcgtatgc atccaccaga aaagccaggg
      781 ttgcgcggtc cgctccggcc gaaggctcga tcacgtaggg cagcaccctc tcgtgagtct
      841 gctcgtcgaa gtaggtgaga tcctccccgc tcacagcctg gtgctggcga agatcgaaat
      901 cggtccgatt ggcgatcccc tcgagctccg accagccgaa ggggaactga tactcgatat
      961 ccaccgtggc cttggcgtag tgcgccagct cagcgggatc gtgggggcga agcttcaggt
     1021 tctccatccg cattccaaat cgctcgtacc accccatccg ctccttcacc cagtgctcgt
     1081 gccactccgt atccgtgccc ggcacgacga agaactcgat ctccatctgc tcgaactcac
     1141 gagtgcggaa ggtgaaattg cccggggtga tctcgtttcg gaacgccttt ccgacctggg
     1201 cgatgccaaa cggcagcttc tttcgagtcg tggtctgcac gttcttgaag ttgacgaaaa
     1261 taccttgagc cgtctccggc cgcaggtagg ttaccgaggc atcgtcctcc acggggccga
     1321 caaaggtctt gaacatcatg ttgaactgcc gggcctcggt cagttctcca ccacaggacg
     1381 ggcagcgatc gccctccacg tggtccgctc ggaaccgttg ccggcacacc ttgcagtcga
     1441 ccaggggatc ggtgaagttg gcaagatggc ccgatgcctg ccacacctgc ggggccatca
     1501 agatggcagc atctatctgg aacatgtcgt ctcgctcctg gactaccgct cgggtccagg
     1561 cactcttgac gttgttcttc aataggctgc ccagcgggcc gtaatcccag gtgctgccaa
     1621 agccgccgta gatctcgctg ttggggaaaa cgaagcctcg cctcttgcag agggagacaa
     1681 tcttgtccat atctggtacg gggcgagtca caattcctcc tcagaaaaaa gaccttctcg
     1741 ccattaaacg gcgagaggtc cccatcgtca cagaatgata tcggagaggc tacacaaagt
     1801 caacgcagag gccgatttcg gcacgcgctc gtgaggagct taccacagaa aatcgagcaa
     1861 atcatcactt ctaacattgt ggcacttgcg agcacatacc gctacggcag aaggcccagg
     1921 agccgcatct catcccattg gtcgggcgtg agcagctcgg gccggcggtg caaggtccga
     1981 aggaggctct gctcccggcg ccatcgcgcg accttcgcgt ggttcccaga cagcagcacg
     2041 tcgggcaccc cccagccctc ggaactcggc gggccgagtg tagtgcggat actccagaac
     2101 accggctgag aacgactcct cctccacaga ctcctcggcc cccagcgccc ccggtatcag
     2161 cctgaccacg gcgtcacaca ccaccagcgc cggcaattcg ccgcccgtga ggacaaagtc
     2221 ccctacgcta atctcctcat ccacgaggct ctctatcacc cgctcgtcca cgccctcgta
     2281 gtgcccgcag agcaggatca agtgcgggag tgtcgagagc cgctgcgcaa tcgtctgtcg
     2341 aaagggctgc ccctgggcgc tcatcaggag aacgtgcgaa tcgtccctct tgacggcctc
     2401 caccgccgcg gtgataggct ccggcttcat caccattccc ggcccgccgc cgaaaggata
     2461 atcatccaca accttgtgac gaccggtggc aaagtcccgg atattgtggg tctggaactc
     2521 aaccaaccct tgctgcgccg ccctcccgag gatgctctcc tggaaaggcc ccacgaacat
     2581 gcccgggaac agcgtcagaa tgtcgatccg caactccgtc ctccctatct aagccacctg
     2641 gcgtccaacc gatccaacgt cccgtcggcc ctcatcgctt ccaactcccg gttgatcgcc
     2701 agaagcagtc caggatcctt tcggcccacc gcaaccgagt agggctgttt ccgcaagggg
     2761 aacgccacag gtttcaactc cgccatggtc gatgtaccca cgggaatcga ggggcggtcc
     2821 actatgatag cgtctgcccc gccgccagcg acctgcgcga gcgccttctc gacgtcgtcg
     2881 acggtaacca cctccacgct gggtgtcgac cgggcaagca gcctggcctc ctcttccccc
     2941 acactgccca actccaccgc caccc
//
LOCUS       DATALS010000073         8533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_231137, whole genome shotgun sequence.
ACCESSION   DATALS010000073 DATALS010000000
VERSION     DATALS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231137"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VHS06_01455"
     CDS             <1..1117
                     /locus_tag="VHS06_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX2986825.1"
                     /translation="SSVGSPARSTYFQLLLRFGVSRTFAVVKCTVGSKGKEHESRFRR
                     RAKVLPPTEELLEMHRQMVLSREFEETLCEIHAKSPIPELPHASTGQEAVGVGVCFRL
                     RPDDVIIPSVRSHPAFLAKGVPTRTLMAGAFGKATSPSGGRNTSHHMGDMSRGIIAGS
                     AVLTSHLPIAVGVALASKMEGKDYVSVAFFGDGSSNRGEFHESLNLAAILKLPVIFVC
                     ENNLHALSIPASYHMSVPNVADRAVAYNIPGVIVDGNDVLAVVDATSEAVARARRGDG
                     PTLMECKTYRWRGHSERDPTDPRPKEEIALWKARCPIARSRRLLIEEVGVEESRVLEV
                     EASAKAEVADAASFAQESPYPVPEDCLTGVYAPAEGE"
     gene            1120..2097
                     /locus_tag="VHS06_01460"
     CDS             1120..2097
                     /locus_tag="VHS06_01460"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX2986826.1"
                     /translation="MARMTMRDAIKEAMREEMLRDPRVFLMGEDIGLFGGVFKVTKGL
                     WEEFGAKRIIEMPISETAIAGAGIGAAMMGMRPIVEIMFADFLTCAMDQIVNNAAKMR
                     YGHGGEAEVPMVIRSTFGAGTRSGMQHCQSVESWFLNVPGLKIVMPSNASDAKGLLKS
                     AVRDPNPVLFLEHKMLYSVWGEVPDGEALVPIGKARVARAGRDLTIVTLGSMVPKVLR
                     VAEQLAGRGVEVEVIDLRSVYPMDTETIVESAAKTARLMIVHEAPVTGGIGAEVAAIA
                     AKECFGYLDAPIERVGAPYIPVPFSPTMEDYYIPGEDRILSTASALVGV"
     gene            2097..3266
                     /locus_tag="VHS06_01465"
     CDS             2097..3266
                     /locus_tag="VHS06_01465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX2986827.1"
                     /translation="MIVPILMSQVTLTMTDGVILRWLVKEGDRVAEGQMLAEVETDKA
                     SVEIPASQEGIIVRFVAKEGDRVEIGQPIAQLEVAGEDGAAPEKPQEPAAETVPAAPV
                     AQKQQDLPVRRRISPVAARLAREHGIDIQLLTATGPGGLIVEADVRCFLEKPAPKPDV
                     DGDAPEETVVPMSAVRRRIAERMLLSRTATATVTTIAEVDMGAVKALRQRIPVSYTVC
                     LIRAAALALRKFPYVNSELRGDNIILKNRVNMGVAVAVDESTLVVPVIRDADKKGLKE
                     IAAELDSLSTSAKAGTIRPEQVTGGTFTVTNSGVWGALMFTPAINYPQAAILGSGKVM
                     DTPVVRDGGIFVRPMMYVCLSYEHRIIDGAVAIRYLQEVKRLLEQPEGALTDETI"
     gene            3460..4539
                     /locus_tag="VHS06_01470"
     CDS             3460..4539
                     /locus_tag="VHS06_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX2986828.1"
                     /translation="MKYLLTFLAASILGVTLLLSGCSQAAPAPTAAPTKPAESTKAPA
                     AQTTAAPQPTAASTAKVDFPQKGKTITLISPWTPGGSTSVGLRLLAPLLEKELGVQVQ
                     VVDKPGAGSQVGLTEAARAKPDGYTIAGANLPAIQAIYLDPERKSAFTRKDFDLVALM
                     VQDPGAVGVKADSPIKSVKDLVDMAKAKPDQVKGAATGIWGLGDLAMYELERATKAKI
                     AHVQFDGSASSMAALMGGHVDSQWGTVGDFLTYVQSGQIRLIGVMDKKESEFAPGVKT
                     FEAQGYNVVLGSSRGIVIPAGTPKEITAKLSAAVKKAMQDPELKKKMSDMSLPLSYMT
                     PEEFGAHWGEMDTMTKELMSAAKKN"
     gene            4688..5761
                     /locus_tag="VHS06_01475"
     CDS             4688..5761
                     /locus_tag="VHS06_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2986829.1"
                     /translation="MTRSVVLAEPYRMEIREFPLPDVGPQDMLLKVEMCGICGGEPKK
                     YTGQSTWEMPFPIILGHEVVGYVQEVGGKAAETYQVQPGDRVTIEPYIGCGFCEFCSA
                     GYYQLCTHQRCYGTSDCADTPPHLFGAYGEHLFVSWGSRVHKVDRGVPAEAAHLSSVI
                     GNGVRWARTKGQVQFQEPVVIIGPGAQGLGAVISCVESGSHPIIVVGVASDKKRLELA
                     REFGAHHTLVNGEVNVAEAVRDITHGKMPRVVLECSGTLPGFELGLELVRPLGRLVMA
                     GVPHGQRANLALQKVPLKEITILGALGQLYSEVADAVKILNSRRYPIEKIVTHVFPME
                     KAEEGMKFFMSKDPSCIRVALKP"
     gene            6042..6818
                     /locus_tag="VHS06_01480"
     CDS             6042..6818
                     /locus_tag="VHS06_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2986830.1"
                     /translation="MAGERRLPVVFVPGGVLPAELSYGKLLPELGDQVDFVLKDLEVY
                     AGDVPPADYELGLEAEAIEQAADDAGFDTFHLVGYSGGGASALVFTAGYPDRVRSLAL
                     VEPAWIGNEGWTTDEAAYWQKIALLMTLQPDERMEGFARLNTGSGKVIPRLGPQPDWM
                     AKRPAGLEAMTRAFERSFLDVESLRNFHRPVYVAVGGLSHPAERDKAERLSSVFPDIQ
                     VEVYQDRHHMDPPQRAEAARFARALHELWRRAGWHQAGIG"
     gene            complement(6888..7862)
                     /locus_tag="VHS06_01485"
     CDS             complement(6888..7862)
                     /locus_tag="VHS06_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX2986831.1"
                     /translation="MTYTVEPDPRLVEKFGRDTVSFYPGFGAGYELKIDPETNTYSDE
                     WGVKYRMPPDGYYFDPFQFPLAKAETPADLDRHRWPDPHDLNRIRGIAEEIKATVGAG
                     EKAILLGAPTLGLWMLPQFLRGMEQALMDLAGNKKLAEALAEKITEWYEEYWDSTLAV
                     VGPYVDFVHMEGDLGDQNGPLFSPKTFREIYKPRLRRVVDAIKRRSSARIWLHSCGSI
                     YWAIPDLIDAGVEVLNPVQVNAAEMDSARLKREFGKDLTFWGGGCDPVVLQCGSPSDV
                     EADVRRRIADFAPGGGFVFASVHNIQANVPPANIVAMFEAAHRYGIYR"
     gene            8209..>8533
                     /locus_tag="VHS06_01490"
     CDS             8209..>8533
                     /locus_tag="VHS06_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986832.1"
                     /translation="MLEVAIVGKEMTPRERVLTAAAHREPDRVPMDFGGRISTIHAYA
                     HRELKKHLGLEGGEEKIVGYMTYTVEPDPRLVEKFGRDTVSFYPGFGAGYELKIDPET
                     NTYSDE"
BASE COUNT         1686 a         2448 c         2823 g         1576 t
ORIGIN      
        1 atccagcgtt gggtcaccag caagaagtac ctatttccag ctgctcctac gcttcggagt
       61 gtcacgtacc ttcgccgtag tgaaatgcac cgtcgggtcg aagggtaagg agcacgaatc
      121 gcgtttcagg aggagagcga aggtgttacc accaacggaa gaactgctgg aaatgcaccg
      181 acagatggtg ctgtccaggg agttcgagga gaccctctgc gagatccacg cgaagtcgcc
      241 cattccggaa ttgcctcacg cgtccaccgg ccaggaggcg gtcggggtcg gcgtctgctt
      301 ccggcttcga cccgacgacg tgatcatccc atcggtgcga agccatccgg ccttcctggc
      361 gaagggggtg cccacccgta cgctgatggc cggcgccttt gggaaggcaa caagcccatc
      421 cggcggcagg aacacttctc accatatggg ggacatgtcc cgggggatca tcgccggcag
      481 cgcagtgctg acctcccatc ttccaatcgc cgtaggcgtg gctctggcct cgaagatgga
      541 gggtaaggac tacgtgagcg tcgccttctt cggtgacggc tccagcaatc gcggcgagtt
      601 ccacgaatcc ctgaacctcg ccgccatctt gaagctgccg gtcatattcg tctgcgagaa
      661 caacctccac gccctgtcca tcccggcctc ctatcacatg tcggtgccga acgtggcgga
      721 tcgggccgtg gcatacaaca tcccgggtgt gatcgtggat ggcaacgatg tcctggccgt
      781 cgtcgacgcc acctccgaag ccgtggccag ggcgaggaga ggggatggcc ccacgcttat
      841 ggagtgcaag acctatcgct ggcgcggcca ctccgagagg gacccaaccg accctcggcc
      901 gaaggaggag atcgcgctct ggaaggcacg gtgtcccatc gcccgctctc gcagactgct
      961 catcgaggag gtgggagtcg aggagagccg ggtcctggag gtggaagcat cggcaaaggc
     1021 ggaggtggcg gatgcggcca gcttcgccca ggagagtcct taccccgtgc ctgaggactg
     1081 tctgaccggt gtatacgcgc ccgcggaggg agagtgacca tggctcgcat gactatgagg
     1141 gacgctatca aggaggcgat gcgcgaggag atgctccgag accccagggt cttcctgatg
     1201 ggcgaggaca tcggactctt tggcggcgtc ttcaaggtaa ccaaggggct ctgggaagag
     1261 ttcggtgcca agcggataat cgagatgccc atctctgaga cggctatagc cggggccggc
     1321 atcggcgcgg ctatgatggg catgcggccc atcgtggaga tcatgtttgc cgatttcctc
     1381 acctgtgcca tggaccagat cgttaacaat gccgccaaga tgcggtacgg ccatggtggg
     1441 gaggccgagg tgccgatggt gatcaggagc acctttgggg caggcactcg ctccgggatg
     1501 cagcattgcc agagcgtcga gtcgtggttc ctgaacgtgc cgggcctcaa gatcgtgatg
     1561 ccctccaacg cttccgacgc caagggattg ctcaagagcg ccgttcgaga ccccaacccg
     1621 gtgctgttcc tcgaacacaa gatgctctac tccgtctggg gcgaggtgcc tgacggggag
     1681 gcgctggtgc cgataggcaa ggcgagggtc gctcgagccg gccgggacct gaccatcgtg
     1741 accctgggtt ccatggtgcc gaaggtactg cgagttgccg agcagctggc cggccgtgga
     1801 gttgaggtgg aggtgatcga cctccggtcg gtgtacccga tggacaccga aaccatcgtg
     1861 gagtcggcgg cgaagacggc gaggctgatg atcgtccacg aggcgcccgt aaccgggggc
     1921 attggggctg aggtggcagc gatcgcggcg aaggagtgct tcggctacct ggatgccccc
     1981 atcgagcggg tcggggcgcc ctacataccg gtgcccttca gccccacgat ggaggactac
     2041 tacattcccg gcgaggatcg catcctcagt acggcttcgg cgttggtagg tgtgtgatga
     2101 tagttccgat cctgatgtcc caggtaaccc tgacgatgac cgacggcgtc attctgcgct
     2161 ggttggtcaa agagggcgat agagttgcgg aaggccagat gctcgcagag gtcgagacgg
     2221 acaaggcatc ggtggagatc cccgcgtctc aagaggggat aatcgtgcgg ttcgtcgcga
     2281 aagaagggga ccgggtggag atcggccaac ccatagcgca actggaggtg gcaggggagg
     2341 atggggccgc acctgagaaa ccccaggagc cagcagcaga gacagttcct gccgcgcctg
     2401 ttgcccagaa gcagcaggat ctcccggttc ggcggcggat ctcgccggta gcggccagac
     2461 tggcgaggga gcatggcata gacatacagt tgctgacggc aaccgggccc ggcggcctca
     2521 tcgtcgaggc agacgtgagg tgcttcctgg agaaaccggc ccccaagccc gatgtggatg
     2581 gggatgcacc tgaggaaacc gtggtgccga tgtctgccgt tcggcgccgc atcgccgagc
     2641 ggatgctgtt gagtcgcacc gctacggcga ctgtaaccac catagctgag gtagatatgg
     2701 gggcagtgaa ggccctgcgg cagaggatcc ctgtctccta cacggtgtgc ctgatccggg
     2761 ctgctgccct ggcgctacgt aagttcccct acgtgaactc ggagctgaga ggcgacaaca
     2821 tcatcctcaa gaacagggtc aacatggggg tggctgtagc ggtcgacgag agcacgctag
     2881 tggtgcctgt gatcagggac gcggacaaga agggactcaa ggagatcgcg gctgaattgg
     2941 actccctctc cacctcggcc aaagcgggaa ccatacgtcc ggagcaggtg acaggaggga
     3001 ctttcaccgt caccaactct ggtgtgtggg gcgccctgat gttcacgccc gcgatcaact
     3061 accctcaggc ggcgatactg ggatcgggga aggtgatgga cacgccggtc gttcgcgatg
     3121 gggggatatt cgtccggccg atgatgtatg tctgtctctc ttacgagcac cggatcatcg
     3181 acggggctgt agctatcagg tatctgcagg aggtgaagag gctgctggag cagcctgaag
     3241 gcgcactcac cgacgaaacg atctagaacg gatcatcaga ccgcggagaa cgatcaatct
     3301 cgtggtcggg tcgtccaccc ggtcaactga gcaactttcc ctggatgcag tcagtctttg
     3361 ggcgagggga aagtcggtac caccaaggga gagttgctta gtccatcagg cagccaacgg
     3421 gtcctactac cagacgtagt attcgcgaag gagagaaccg tgaagtacct gttgacgttc
     3481 ttggcagcca gtatcctcgg tgtaaccctt ttgctctccg ggtgttccca ggccgcccca
     3541 gcgccgactg cggctccgac gaagccggcg gagtcgacca aggcgccggc cgcacaaacg
     3601 acggccgcgc cacagcccac cgccgcgtcg accgcgaagg tggactttcc ccagaagggt
     3661 aagactatca ccctgatctc gccatggacg ccgggtggat ccacttcggt gggtctccga
     3721 ttgctggccc ccttgctcga gaaggagttg ggcgtacagg tgcaggtggt ggacaagccg
     3781 ggtgcaggct cccaggtggg cctgacggaa gcggccagag ctaagcccga cgggtacacc
     3841 attgccggtg caaacttacc agccatccag gccatctatc tggaccctga gcgcaagtcg
     3901 gccttcaccc gcaaggactt cgacctggtg gcgctcatgg ttcaagatcc aggcgctgta
     3961 ggcgtcaagg ccgacagccc tatcaagagt gtcaaggatc tggtcgacat ggcgaaggcg
     4021 aagcccgacc aggttaaggg ggcggctaca gggatctggg gcctcggcga cctggccatg
     4081 tacgagctgg agcgggcaac gaaagcgaag attgcgcacg tgcagttcga tgggtctgcg
     4141 tcgtccatgg ctgccctgat ggggggccac gtggactccc agtggggaac cgttggagac
     4201 ttcctgacct acgttcagag cggccagatc cgtctgatcg gtgtcatgga taagaaggag
     4261 agcgagttcg cgccgggggt gaagacgttc gaggcccagg gctacaacgt cgtcttgggc
     4321 tccagccgtg gcatagtgat cccagccggc actccgaagg aaatcaccgc caagctgtcc
     4381 gccgcggtaa agaaggcgat gcaggaccct gagctgaaga agaagatgag cgacatgagc
     4441 ctcccgctca gctacatgac tcccgaggag ttcggggctc actggggtga gatggacacg
     4501 atgaccaagg aactgatgtc cgcagccaag aagaattagg caacgccgtc tttgtcgaag
     4561 ttgcgccgga tttccggcgc aacttcgaca aagatctggt gaaccgcgag gcaatccggc
     4621 cccaaggagt gggcagggtg acctatcaga aagtcttgat ggatggaggc cagtaggcgt
     4681 ggtcgaaatg accagatcag tggtgttggc tgagccctat cgcatggaga tccgggaatt
     4741 cccccttcca gatgtcggcc cgcaggacat gctgctcaag gtggagatgt gcgggatctg
     4801 cggaggggag ccaaagaaat atacggggca gtcgacctgg gagatgccct ttccaatcat
     4861 tctcgggcac gaggtggttg gctacgttca ggaggttggg ggaaaggcgg cggagaccta
     4921 ccaggtccag cctggcgacc gggtcacgat cgaaccctac atcggctgcg gcttctgcga
     4981 gttctgctcc gcaggctact accaactgtg cacccaccag cgctgctacg gcacgtcgga
     5041 ttgtgccgac accccccctc acctcttcgg cgcgtacggc gagcatctct ttgtatcgtg
     5101 ggggtcgagg gttcataagg ttgatcgggg cgtcccggcg gaggcggctc acctctccag
     5161 tgtgattggc aacggtgtcc ggtgggcgcg gacgaagggg caggtccagt tccaggaacc
     5221 ggtggtcatc atcgggcctg gggctcaagg gttgggggcc gtcatctctt gcgtagagtc
     5281 gggatcgcac ccgatcatcg tggtaggtgt ggccagcgac aagaaacggc tggaactggc
     5341 ccgagagttc ggcgctcacc atacgctggt gaacggcgag gtgaacgtgg ccgaggccgt
     5401 acgggacata acacacggga agatgccgcg ggtggtgctg gagtgttcgg ggaccctgcc
     5461 ggggttcgaa ctgggtctcg agcttgtgag gccgttgggc cgtctggtca tggccggcgt
     5521 tccacacggg cagcgggcaa acctagcgct tcagaaagtg cctctgaaag agataacgat
     5581 cctgggtgcc ctcggacagc tctactcgga agtggcggat gcggtgaaga tcctcaactc
     5641 gcggcggtat cccatcgaga agatagttac tcacgtcttc ccgatggaga aggccgaaga
     5701 gggcatgaag ttcttcatga gcaaggaccc aagctgcatc cgagtggcgc tcaagccctg
     5761 aaacggatct gatcgcgcga tctgttgtgc tgagccttcg ggaacgcgaa gcctatgtcg
     5821 gtagatttgg cgttcccagg cgttcgcaag gcagagctgt tgccggggtg gggtggggga
     5881 caagtccgca ccgacaactt tatcatagag ccaggccgaa aaccggcctg gctcagcgtt
     5941 tgttcggcag aaaagggctg gggcattctt cttgctggtt cgtgtggcgg tgggggcttg
     6001 cccggcgata gcaggcgcat cgaaaggggg gcggcgaaac gatggcaggg gaacgacgtc
     6061 ttcccgttgt gtttgttcct ggcggggtgc tgccggcaga actgtcctac gggaagctgt
     6121 tgccggaact cggagatcag gtagacttcg ttctcaaaga tctcgaggtc tacgcggggg
     6181 atgttccccc ggccgactac gaactgggtc tggaggccga ggccatcgag caagctgcgg
     6241 acgatgccgg tttcgacacc ttccacctgg tcggctactc cggcggagga gcctctgcac
     6301 tcgttttcac ggccggctat ccggatcggg tgaggagcct cgccctggta gagcccgcgt
     6361 ggatcggcaa cgagggctgg actaccgacg aggccgccta ctggcagaag atagccctgc
     6421 tcatgacgct gcaaccagac gagcggatgg agggcttcgc gcggctgaac accggctcgg
     6481 gcaaggtgat tcctcgattg gggccgcagc ccgattggat ggccaagcgt cccgccggcc
     6541 tcgaggcaat gacgcgagcg ttcgagcgtt ccttcctgga tgtggagagc ctccgcaact
     6601 tccaccggcc cgtctacgtc gcggtgggag ggttaagcca ccctgcggag cgggacaaag
     6661 ccgagaggct ctcctctgtc ttcccggaca tacaggtaga ggtttaccag gatcgccatc
     6721 atatggatcc gccacagagg gcggaggccg ctcgtttcgc tcgagcgctc cacgaactgt
     6781 ggagacgagc ggggtggcac caagcaggaa tcggctaaca acgcctagcc ttgtggtcgc
     6841 accgttcttc taagtaggta gcagaaagaa ggaatcacgg aagccgtcta cctgtagatt
     6901 ccgtatcggt gtgccgcctc gaacatggcc acgatgtttg ccggcgggac gtttgcctgg
     6961 atgttgtgga ccgaggcgaa gacgaagccg cctcctggcg caaagtcggc aattcgcctt
     7021 cggacgtcgg cctccacatc ggatggcgat ccgcactgta gaaccacggg gtcgcaaccg
     7081 ccgccccaga aggtgagatc ctttccgaat tcccgcttta ggcgggcact gtccatctcg
     7141 gcggcgttga cctgtaccgg gttgagcacc tcgacacccg catcgatgag gtccgggatc
     7201 gcccagtaga tcgagccgca ggagtgcagc cagattcgag cggaggaccg acgcttgatg
     7261 gcatcgacca cgcgtcgcag tctaggcttg tagatctccc tgaaggtctt cggggagaag
     7321 aggggaccgt tctgatcgcc gaggtctccc tccatgtgca cgaagtcgac ataaggtcct
     7381 accaccgcga gcgtactgtc ccagtactcc tcgtaccatt ctgttatctt ctcggccagc
     7441 gcctccgcca gcttcttgtt gccggccagg tccattagcg cctgctccat ccctcgaagg
     7501 aactgcggca gcatccagag gcccagggtg ggagctccca gcaggatcgc cttctcacct
     7561 gccccgactg ttgcctttat ctcctcagcg atccctcgta tgcggttgag atcgtgtggg
     7621 tccggccatc gatgccgatc gaggtctgct ggagtctcgg ctttggccag agggaactgg
     7681 aaagggtcga agtagtagcc gtccgggggc atccggtact tgacccccca ctcgtccgag
     7741 taagtattcg tctcggggtc gatcttgagc tcgtagcccg cgccgaagcc cggatagaag
     7801 gagacggtat ctcgcccaaa tttctccacc aacctggggt caggctccac ggtataggtc
     7861 atgtagccgg ccctccgtat cctgtcagga ttctgttagc atctctccac ccccagatgc
     7921 cggctcatct cgagttccat gactgccttg cccgatgccc ttaccccagg gcctcctagt
     7981 tgttaccctg agcgcagcga agggtctcca cgcacgtacg aggagattct tcgctcacgc
     8041 tcagaatgac agggtgatag ccaccacgcc gcccttggcg ggttcggcgc tgcagaacca
     8101 aaaggccctc cttctgggct ggcataggcg agccatgtag atgcataatg aggaacatgc
     8161 ctcgctctgc ttgcgattgg acgcatcggg tcgaatcaaa aggacggaat gctggaggta
     8221 gctatcgtgg gtaaggagat gacccctcga gagagagtgt tgactgccgc ggctcacaga
     8281 gagccggacc gtgtccctat ggatttcggc gggcgcatca gcaccattca cgcctatgcc
     8341 catcgggaac tgaagaagca cctcggcctc gagggcggcg aggagaagat cgtcggctac
     8401 atgacctata ccgtggagcc tgaccccagg ttggtggaga aatttgggcg agataccgtc
     8461 tccttctatc cgggcttcgg cgcgggctac gagctcaaga tcgaccccga gacgaatact
     8521 tactcggacg agt
//
LOCUS       DATALS010000074         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_6293,
            whole genome shotgun sequence.
ACCESSION   DATALS010000074 DATALS010000000
VERSION     DATALS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6293"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VHS06_01495"
     CDS             complement(<1..198)
                     /locus_tag="VHS06_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018843788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX2986833.1"
                     /translation="MRLPAVFQRYRREIDRELRSALADRTFPMYDMLRYHMGWIDGEG
                     NPLPVSSGKALRPTLCLLACEA"
     gene            complement(195..2096)
                     /gene="dxs"
                     /locus_tag="VHS06_01500"
     CDS             complement(195..2096)
                     /gene="dxs"
                     /locus_tag="VHS06_01500"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX2986834.1"
                     /translation="MASMLESVDSPAQLKDLSYPELDRLSDDIRSELIESVAQTGGHL
                     ASNLGVVELTIALHRVFDSPSDKIVWDVGHQAYVHKLLTGRREKFGSLRQYGGLSGFT
                     DRAESPHDPFGAGHASTALSAALGMALARDLAGASNHVLAVIGDGSFGGGMAFEALNH
                     AGHLGTRLIVVLNDNGMSISPSVGALSKLLNSVRLDSKYEMAKAETKRAMIHLPMGSS
                     FYAAGKRVKSGLKRALIPSSLWEELGFVYIGPVDGHDIREVEAALRRARDHETRPTLV
                     HVLTKKGKGHQPAEADATKYHGLSPKGSRSGSAPSYSDVFGRTIYRLMREDSRIVAIT
                     AAMLDGTGLALAAGKFPHRVFDVGICEQHAVTLAAGLATQGVIPVVAIYSTFLQRAYD
                     QIVHDVCIPRLPVVFAVDRAGIVGDDGKTHHGAFDVSYLSGIPNMVVSAPKDEDELRQ
                     LIYTATISGLPMAVRYPRDRGRGVTMENALRALPIGRGELLRDGGDVGILALGSTVYP
                     AMEAAERLAAKGIDSAVVNARFAKPLDVELIREIARKTGRILTAEEGVISGGFGTAVL
                     RLLEESGLPEVRVECLGLPDRFVEHGPRDLFLKMFDLDAEGIARRAIDAFPELWGRPA
                     IRMVRGGAE"
     gene            complement(2217..>2747)
                     /locus_tag="VHS06_01505"
     CDS             complement(2217..>2747)
                     /locus_tag="VHS06_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986835.1"
                     /translation="PRFPVRVVPVGITYDFMARGRQQVFVNVGPEMTGLRGLRPAEAS
                     ARVAEAILAQSTITASQLASRLLLKVSAVGGDLISREQLIEYVMSEAERWGRTGVHLD
                     PRLTERGRLEERLEDYLEYCLGSGELVFRDRDGFLIQASPERPVNGSNGNGVLCYIDN
                     ELTSLADGLRGYAAIN"
BASE COUNT          537 a          922 c          786 g          502 t
ORIGIN      
        1 ggcctcgcag gccaggagac agagggtggg ccgaagcgct ttccccgacg acaccggcag
       61 cgggttccct tcgccgtcga tccatcccat gtggtagcgc agcatgtcgt acatcggaaa
      121 ggtgcggtca gccagcgccg agcgaagctc tctgtcgatc tctctccggt agcgctgaaa
      181 cactgctggc agtctcactc cgcccctcct cgcaccatcc gaatcgcggg tcgcccccat
      241 agctcaggga aggcatcgat ggcccgccgg gctatgccct cggcatccaa atcgaacatc
      301 ttcaggaaca ggtccctcgg tccatgctcg acgaaacggt cgggcagccc caggcattcc
      361 accctgacct ccggaagccc cgattcctcc agaagcctca gcacagccgt cccgaagccc
      421 ccggagatca ccccctcctc cgcggtcaag atccgccccg tcttgcgggc aatctcccgg
      481 atcagctcaa catccagcgg cttggcgaag cgggcgttga ccacggcgct atcgatcccc
      541 ttcgctgcca gccgctcagc ggcctccatt gcagggtaga cggtggaacc gagggccagt
      601 atcccgacgt cacccccatc ccgaaggagc tcgccccgcc cgatgggcag ggcacggagc
      661 gcattctcca tcgtcacgcc gcggccacga tccctgggat aacgcacagc catgggcaaa
      721 ccggaaatgg tggccgtgta gatcagctgc cgcagctcat cttcgtcctt gggggccgag
      781 acaaccatgt tgggtatacc gctcagatag gagacgtcga aggcgccgtg gtgggtcttc
      841 ccgtcgtcgc ccacgatccc ggctcggtcc actgcaaaca ccaccgggag ccgcgggatg
      901 cagacatcgt gcacgatctg gtcataggca cgctgaagga aggtggagta gatggccacc
      961 acaggtatca ccccttgcgt ggccagccct gcggccagcg tgaccgcgtg ctgctcacag
     1021 atccccacgt cgaacacccg atgagggaac ttgccagcag ccagcgcgag gccggtgccg
     1081 tccagcatcg ccgcggtgat ggcaacgatc cggctatcct ctctcatcag tcgataaatg
     1141 gtccggccga agacgtcgct gtagctgggg gccgagccgg acctggagcc tttcggcgag
     1201 aggccgtgat actttgtggc atcagcttcg gcaggttgat ggccctttcc cttcttggtg
     1261 agaacgtgga ccagggtggg acgagtctcg tgatccctgg ctcgcctcag cgccgcctcc
     1321 acctcgcgga tgtcgtggcc gtccaccggc ccgatataga cgaagcccaa ctcctcccag
     1381 agcgagctgg ggatcaaggc ccgcttgagg ccgctcttga cccgctttcc ggcggcatag
     1441 aaggagctcc ccatcggaag atggatcatc gcccgtttgg tttcggcttt ggccatctcg
     1501 tacttgctgt cgagcctgac ggagttgagc agtttggaga gagcaccgac gctgggagag
     1561 atggacattc cgttgtcgtt caggactacg atcagcctgg taccgagatg gcccgcgtgg
     1621 ttcaacgcct cgaaggccat ccccccgccg aaggacccgt cgccgatgac ggccagcacg
     1681 tgattgctcg cccccgccag gtccctcgcc agcgccatcc ccagtgccgc cgacagggcc
     1741 gtggaagcgt ggcccgcacc aaatgggtcg tgagggctct ccgcacgatc ggtgaagccg
     1801 gacaaccccc cgtactgtct caagctgcca aatttttccc gccgtccggt cagcagcttg
     1861 tgcacgtatg cctggtggcc gacatcccag acgatcttgt cactggggct atcgaaaacc
     1921 cgatgcagtg cgatggtaag ctccaccaca ccgaggttgg aggcaaggtg gccgcctgtc
     1981 tgcgccacgc tctcgatcaa ctcagatctt atgtcgtccg atagtcgatc aagctcggga
     2041 taagataagt ccttcaattg ggcgggggag tccaccgatt ccagcatcga cgccatttga
     2101 cactacccca tgtcgcatat tgggcttgcc aaaagggaag gtgaagtgaa ggcctaatca
     2161 ctgacgagga tcaactgcat atcacatgcc agtaacagtg gatactgcca tagctgtcaa
     2221 ttaatcgccg catatcctct taacccatcc gccaggcttg ttaactcgtt atctatgtaa
     2281 caaaggacac cgttaccgtt tgaaccgttg acgggtcgct cgggcgaagc ctgtatcagg
     2341 aagccgtcac gatcgcgaaa gaccagttcg ccggacccaa gacagtactc cagataatcc
     2401 tccagccgtt cctccagccg gccgcgctcc gtcagccggg ggtccaggtg cacgccggtc
     2461 ctgccccatc gctcggcttc agacatcacg tattcaatca actgttcccg actgatcaga
     2521 tctccaccca cggcgctaac cttcagcagc aaccgactgg caagctggct ggcagtaatc
     2581 gtggactggg ccaggatggc ttccgccact cgagcggatg cctcggccgg tctcaatccg
     2641 cgcagtcccg tcatctccgg ccccacgttc acgaatactt gctggcggcc tcgagccatg
     2701 aaatcgtagg tgatgcccac gggcaccacc cgcacaggaa acctcgg
//
LOCUS       DATALS010000075         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_366053, whole genome shotgun sequence.
ACCESSION   DATALS010000075 DATALS010000000
VERSION     DATALS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366053"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VHS06_01510"
     CDS             <1..212
                     /locus_tag="VHS06_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986836.1"
                     /translation="VAQLNHIQESPAIGVPMSIPYMAIPVGSLLGIIFVWLDVLIHWN
                     TAREASDTNAEDVAAGSHAIVEEVG"
     gene            212..664
                     /locus_tag="VHS06_01515"
     CDS             212..664
                     /locus_tag="VHS06_01515"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX2986837.1"
                     /translation="MVAWIMFLSFLILLFLSFPVAVSMGLACVFAVLSQGNTSITTIA
                     QKMFTGLDSFPLLAVPLFILAGGFMETGGISTRLVTFANVLVGWIRGGLGMVSIVGTI
                     FFSGISGSSAADTAAIGSIMIPSMSKRGYPRPLATAIVASAGAMGIEK"
     gene            complement(706..903)
                     /locus_tag="VHS06_01520"
                     /pseudo
     CDS             complement(706..903)
                     /locus_tag="VHS06_01520"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
     gene            complement(950..>2081)
                     /locus_tag="VHS06_01525"
     CDS             complement(950..>2081)
                     /locus_tag="VHS06_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX2986838.1"
                     /translation="MARWSFSESRKARSVVGLTLVGAMAALVVACSSGAPAAQPTSAP
                     AKPAAAATSAPAAAATKAPAAAATTAPAAGAVVTKFASVQAEDHAHHLSYLFFAQRVK
                     ELTGGKLIINIYPNSQLGNEREYIEQLRTGQIEFARVATAVLGPFVPQFQAFDLPYLF
                     KSKAQMLSAADGELGALLQKQLQEKLGIRGLGFLDDGTRSLYNNKRPVKTLADLKGMK
                     VRVMENQLMIKTFNTLGAAATPMAYGEVYSALQQGVIDAAEGPINSYWAVKHFEVAKY
                     FSFTDHFISPALPMVAKKWYDAQPKEFQTAIDQAGKEMSMKSRELQNAQEKSAETELK
                     AKGSQINQVDDKAPFMKAVEPLYAEYEGKIGKDVIEMARNAK"
BASE COUNT          363 a          607 c          649 g          462 t
ORIGIN      
        1 atgtggctca gctaaaccac attcaggagt cgcccgctat cggcgtgccg atgagcatcc
       61 cctacatggc tatcccggtg ggatccctgc tggggataat cttcgtatgg ttggacgtct
      121 tgatccactg gaataccgct agggaggcat cggacacgaa cgctgaagac gtggcggccg
      181 gatcccatgc catcgtcgag gaggtaggat aatggtagcc tggatcatgt tcctgtcctt
      241 cctcatactg ctgttcctgt ccttcccagt ggccgtgtcg atggggctgg cctgcgtctt
      301 cgcggtactg tctcagggca atacgtccat caccacgatc gcgcagaaga tgttcacagg
      361 gttggactct tttcctctgt tggcggtgcc tctgtttatc ctggccggcg gcttcatgga
      421 gacaggaggc atatcgacac ggctggtgac cttcgccaac gtcctggtcg gctggatccg
      481 gggcgggctg gggatggtat ccatcgtcgg gaccatcttc ttctcgggga tctccggttc
      541 ctcggccgcg gacaccgcgg cgatcggttc gatcatgatc ccctccatgt ccaaacgagg
      601 gtatcctcgc cccttggcca cggcgatcgt ggcctccgcg ggcgccatgg ggatcgagaa
      661 gtgagtgcgg tccttgaaag ccattgcagc cccgagaagg gccacccaga tgaacaggaa
      721 gcgagcggtt tcctccgtcc agtggagggg ttggttgatg aaatagcgga atactacctg
      781 taggaagatg acaatcgtcg tgacggccag caagatggcg gcaatgacgg tgacaatcag
      841 tcccagccaa cggttaaagg ctttgatggc gttaatggca cacctcccga gtaacaagcc
      901 catctatcga cgagcccgga gagggatcct ctccgggctc gtgtgagtgc tacttggcgt
      961 tcctcgccat ttctatgacg tctttgccga tcttgccctc atactcggca tagagtggct
     1021 cgactgcctt catgaatggg gccttgtcgt ccacctggtt gatctgagag cccttggcct
     1081 tcaactcggt ctccgcgctc ttctcctgag cgttctggag ttcgcgcgac ttcatcgaca
     1141 tttccttgcc ggcctggtcg atggcggtct ggaactcctt gggctgagcg tcgtaccact
     1201 tcttcgctac catcggcagg gcgggggaga tgaagtggtc ggtgaaggag aagtacttgg
     1261 ctacctcgaa gtgcttgacc gcccagtagg agttgatcgg gccctcggcg gcatcgatca
     1321 caccctgctg cagggccgaa tacacttcgc cataggccat cggggtggcc gctgcgccca
     1381 gggtgttgaa ggtcttgatc atgagctggt tctccatgac acggaccttc atgcccttca
     1441 ggtctgcgag cgtcttcacg gggcgcttgt tgttgtagag gcttcgggtt ccgtcatcca
     1501 ggaagccgag ccccctgatg cccagctttt cctgcaactg cttctggagc agggcgccta
     1561 gctcgccatc ggcggcgctc agcatctggg ccttgctctt gaacaggtaa ggcaggtcga
     1621 aagcctggaa ctgaggaacg aagggaccga ggacagcagt tgccacacgg gcgaattcga
     1681 tctggccggt tcgaagctgc tcgatgtact cgcgctcgtt gccgagctgg gagttagggt
     1741 agatgttgat gatcaacttg ccgcccgtca actccttgac cctctgggcg aagaacagat
     1801 aggacagatg gtgcgcatgg tcttccgctt gaaccgacgc aaacttggtg accactgccc
     1861 ctgcagcagg ggcagtggtt gctgccgccg ccggggcttt ggttgccgcc gctgccggag
     1921 cgctggtcgc tgcggctgcc ggctttgccg gcgcggacgt gggttgcgcc gcgggggctc
     1981 cactactgca tgccacgacc aatgcggcca tggcaccgac cagggtcagc ccgacgacac
     2041 ttcgggcctt gcgggactcg ctgaaggacc atctcgccat t
//
LOCUS       DATALS010000076         3449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_186225, whole genome shotgun sequence.
ACCESSION   DATALS010000076 DATALS010000000
VERSION     DATALS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186225"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VHS06_01530"
     CDS             complement(<1..183)
                     /locus_tag="VHS06_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986839.1"
                     /translation="MNETLTGGVAAVQASHRFGRHAWLSTPGSWPRSLAIALLCLVGV
                     RLFLTVEMALLAFQIPV"
     gene            complement(330..608)
                     /locus_tag="VHS06_01535"
     CDS             complement(330..608)
                     /locus_tag="VHS06_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986840.1"
                     /translation="MELAIQIMFLVLGFCVALFVGGMLTLNRTKGVPKGLSVDDGAVR
                     LDDVGRGKRLQRSAPDAHPETGQSESEESETEAAIKRPATPGKRKRKR"
     gene            799..1584
                     /locus_tag="VHS06_01540"
     CDS             799..1584
                     /locus_tag="VHS06_01540"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX2986841.1"
                     /translation="MESVDNHAIERAAELVRRSSKIVVFTGAGISTESGIPDFRSPGG
                     IWDRYKPVEFGDFVASAEARKRYWIRSRETYPVLASAQPNPAHTAIARLEKMGKLDCI
                     ITQNVDGLHQKAGNSPERVIELHGNTHWVVCLNCSHRSPRADWQPILESGVTEPRCPD
                     CGGMLKVLTVSFGQPMPAAETVEAERRSRRCDLFIVVGSSLVVYPAAQMPVYAVRSGA
                     ALIVVNATSTSMDGSAEVVLREKAGTVLPSIVELAAAGSHLPE"
     gene            complement(1635..2555)
                     /locus_tag="VHS06_01545"
     CDS             complement(1635..2555)
                     /locus_tag="VHS06_01545"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX2986842.1"
                     /translation="MKILVVGPGAMGTLFGGLLVGGGHDVWLLGRRKEVIASIARKGV
                     TVVRGGSRRVFKVQATLNAADAGSVDLVIVLVKAYDTLQACRDALPAVGPETVVLTLQ
                     NGIDNVSSLATVFGRERVLAGVTAQAATLMGPGLVRHAGEGSTSLGELDGQESERVKR
                     IAEALRRSGIEVELTESISSQIWGKLVVNAAINPVTALLRVRNGRLLDWPYTRSLMSA
                     VAREVVAVADAQGIALPYDDPVARVETVCKLTASNRSSMLQDVERGVRTEIDQINGAV
                     VKEGEARGIPTPVNWTLTQLVRALSEAIEG"
     gene            complement(2561..3403)
                     /gene="panB"
                     /locus_tag="VHS06_01550"
     CDS             complement(2561..3403)
                     /gene="panB"
                     /locus_tag="VHS06_01550"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX2986843.1"
                     /translation="MDKKKLTIRELQEMKRQGHKIRMITAYDYPMANLVDQSPMEMIL
                     VGDSLGMVVLGYESTVPVTMEEMIHHTKAVMRGAHNTFVVADLPFMSYQVSVPEAIRN
                     AGRLVKDGGADAVKLEGGESVLPAVRGIVAAGIPVMGHLGLTPQTATMLGGLKSQARD
                     AEAARVLLRDAKLLEEAGVFSLVLEAIPAQLGELVTKELSIPTVGIGAGPGCDGQVLV
                     IHDLLGLFQRFLPKFVKRYANLGESVATALEQYCQEVESATFPAAEHCFNMKREELAK
                     LLEG"
BASE COUNT          697 a         1144 c          979 g          629 t
ORIGIN      
        1 aacggggatc tggaaggcga gcagcgccat ctcgaccgtg agaaacagcc tcacccccac
       61 caggcagagg agggcgatag ccagagagcg aggccaactc cccggagtcg acaaccaggc
      121 atggcgaccg aagcggtgcg aagcttggac tgccgcaact ccgccggtca acgtctcatt
      181 caacactgct ctccacagac acccggtctt gtgtcaacga cgaatctacc gaccgatggt
      241 agggtcggac caacgtgtcc gccctcgccc caatgcccaa ctgtcgacac ccgacagggg
      301 aaggccacac gccccgctcg gcaacgtcgc tatcgcttgc gttttcgctt gccaggggtc
      361 gctggccgct taatggcggc ctcagtctcc gactcctcgc tctccgattg gcctgtctcc
      421 gggtgagcgt ccggtgcaga gcgctgcagc cttttgcccc tgcccacatc gtccaatcga
      481 acggcaccgt cgtcgacgga gagccccttg gggacgcctt tggtgcggtt caacgtcagc
      541 attccgccga caaagagggc aacgcagaac cccagcacta gaaacatgat ctggatggca
      601 agttccatga gtgtagaaaa accctcccgc cattagcttc cgcgccctcc acttcgccat
      661 gcgcaggaga gtggagtggt actcacaaaa tagtatagcg gccggaggac cgtgccgcac
      721 catgggaaag gagtcctaat ccacccgaat acgagccctg gtgctagaat ggcagcatcg
      781 ggacatgagg aggcggtgat ggagagcgtg gacaatcacg cgatcgagag ggcagcggag
      841 ttggtgcgcc gctcatccaa gatagtcgtg ttcaccggcg cgggcatcag cactgagtcc
      901 ggcattccgg actttcgcag cccagggggg atatgggacc ggtacaagcc ggtggaattc
      961 ggcgatttcg tggccagtgc agaggcgaga aagcgctact ggatccgtag ccgtgagacc
     1021 taccctgtcc tggcctccgc gcagccgaat cccgcccaca cagccatcgc ccggctggaa
     1081 aagatgggga agctcgactg cataatcacc cagaatgtgg acgggctcca ccagaaagcc
     1141 ggcaacagcc cggagagggt gatcgaactt cacggcaaca ctcactgggt ggtctgtctc
     1201 aactgtagcc atcgatctcc acgcgccgat tggcagccga tcctggaatc tggggtgacg
     1261 gagccgcgat gtcccgactg cggtgggatg ctgaaggtac tcacggtctc atttggacag
     1321 ccgatgccgg cggccgaaac cgtggaggcg gagcggaggt ccaggaggtg cgacctcttc
     1381 atcgtggtgg gttcttcgtt ggtcgtatat ccggcagccc agatgccggt ctacgcagtg
     1441 cgctccggtg cggcactgat agtggtgaac gccacctcca ccagcatgga cggcagtgct
     1501 gaagttgtcc ttcgagaaaa ggcagggaca gtgttgccct ccatcgttga actagccgcc
     1561 gccggcagcc atcttccaga ataggcgtag gggcgacgca tgcgtcgccc ctacgggaat
     1621 ggacaaaccc acgcctatcc ctcaatggct tcgctcagcg ccctcaccaa ctgtgtcaac
     1681 gtccagttca cgggtgtagg aatgcccctc gcctcaccct ccttgaccac agccccgttt
     1741 atctgatcga tctccgtgcg aactccccgc tccacatcct gaagcatgga ggatctgttg
     1801 gaggcggtca gtttgcacac cgtctccacc ctggcaaccg gatcatcgta gggtaaggct
     1861 ataccctggg catctgcgac cgcgacgacc tcccgagcaa cggcgctcat caacgatcgg
     1921 gtgtacggcc aatccaacaa ccgtccattg cgaacccgca gcaacgccgt gaccggattg
     1981 atggcggcat ttaccaccag tttcccccaa atctgggagc tgatagactc agtaagttcc
     2041 acctcgatgc cggaccggcg cagagcctct gcaatcctct tcacacgctc gctctcctgc
     2101 ccatccagct ctccgagaga ggtgcttccc tcgccggcat gacgaactag cccggggccc
     2161 atcagagtgg cagcctgcgc cgtgactcct gcaagaaccc tttctcgacc aaagacggtc
     2221 gccagggacg agacgttgtc tatgccgttc tgcagcgtca ggactacggt ttccggtcca
     2281 acggcgggca gcgcatcacg gcacgcctga agcgtatcgt aggccttgac caggacgata
     2341 accaggtcga ccgaaccggc gtcggcagcg ttcagcgttg cctgaacctt gaagactcgc
     2401 cgagagccgc cccgaaccac cgtcacaccc ttcctggcga tagaggcaat cacctctttc
     2461 cgacggccaa gcagccacac atcgtggccc ccgcccacca gtaaccctcc aaaaagggta
     2521 cccattgcgc cggggccgac cacaagaatc ttcactcgcg ctatccttcc agcagcttgg
     2581 ccagttcctc tcgcttcatg ttgaagcagt gctcggcggc cgggaatgtc gcactctcca
     2641 cctcctggca atactgctcc aatgcggtgg cgacgctttc tccgaggttg gcgtagcgct
     2701 tcacaaactt ggggaggaag cgctgaaaca accccaatag gtcgtggatt accaggacct
     2761 gtccgtcgca cccaggtccc gcgccgatgc ccaccgtcgg aatggagagt tccttggtca
     2821 ccaattcgcc caactgggca gggatagcct ccaacacgag cgagaagacg cccgcctcct
     2881 ccagcaactt ggcgtcccgc agcaacaccc tggcggcctc ggcatccctg gcctgggact
     2941 tgaggccgcc caacatcgtc gcagtctgcg gcgtgagccc caggtgtccc atcacgggga
     3001 tgcccgcggc cacgatcccg cgcacggcgg gcaggaccga ctcgccgcct tccagcttca
     3061 cggcgtccgc cccgccatcc ttaacaaggc ggcccgcgtt gcggatcgcc tcaggcacgc
     3121 taacctgata tgacatgaag ggcagatcgg ccacgacgaa ggtgttgtgt gcccccctca
     3181 tcaccgcctt ggtgtggtgg atcatctctt ccatcgtcac cggaacggtg ctctcgtacc
     3241 ccagcaccac catgcccagg gagtcaccta ccaggatcat ctccatggga ctctgatcga
     3301 cgaggttggc catcgggtaa tcgtaggcag tgatcatccg gatcttgtgg ccctgcctct
     3361 tcatttcctg tagctcacgt atcgtcagct tcttcttgtc catcctggcc tcctacgact
     3421 tgaaccgggt taagtttagc cgtgtgcac
//
LOCUS       DATALS010000077         8500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_186447, whole genome shotgun sequence.
ACCESSION   DATALS010000077 DATALS010000000
VERSION     DATALS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186447"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VHS06_01555"
     CDS             complement(<1..869)
                     /locus_tag="VHS06_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986844.1"
                     /translation="MKIVVRCTALVLFVLVSALGAMAGPTPSASRVWDVKDPLFEELL
                     SKTSSAGRDQRNGAIYWFHSGNASNLRPFAKEHGIRYALEEQFKELTQRRLLPICQRG
                     NLQDENYRKMADRYRFMVLYQPDYRSSKKEGVPVIEGLPYVLDPRSKAAYFHDLKTAI
                     ETYHKYIWGIYTQDEVHERATRQGVLFFQQMKDTYPYIRKVDEQVKREFGFGKYGIPT
                     SLTDENPYRWIAYHKWVNKQILNWQIETFRMTRRLAPEMRVISVDPVAGHNPFHLDAY
                     GPYVDIATHQLYPS"
     gene            complement(919..1977)
                     /locus_tag="VHS06_01560"
     CDS             complement(919..1977)
                     /locus_tag="VHS06_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020620401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2986845.1"
                     /translation="MSKVVRVGVIGQGRSGYSIHVRGLREMTDRYQVVAVADPLDGRC
                     DDAVEQLGAAGYRDYREMLKRDDLDLVVNATPSHLHVPVCMEIMDSGHNVLCEKPLAR
                     YASEVDQLIAKSKETGKLFAIYQQSRFAPYFRKIREVIESGVLGRIVMVRIAFNGFGR
                     RWDWQTLQDHNAGNLLNTGPHPMDQALQLFGTDVMPEVTCIMDRANCFGDAEDFVKII
                     LKGKGRPVIDVEISSCCAYPTHMYNIDGTLGGLKGTANHLEWKYFKPEEAPQQHLCRE
                     PLPDRAYCSEKLEWHEGSWDLPQENSDWFQYMAVTFYTHLYETIVNGAPLVIPPEQVR
                     QQIAVIEECHRQNPLPRM"
     gene            complement(2306..2647)
                     /locus_tag="VHS06_01565"
     CDS             complement(2306..2647)
                     /locus_tag="VHS06_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986846.1"
                     /translation="MVGVGNNACHTMAAIGLIPLGLMYYAPFSAIVVLGQQYLPNRVG
                     FASGVTLGLAVSVGGIVAPFLGRWADLHGVQSALLCVAALPVLCALIAFTLPAPGKGR
                     GRDDAVPAPRR"
     gene            complement(2683..3561)
                     /locus_tag="VHS06_01570"
     CDS             complement(2683..3561)
                     /locus_tag="VHS06_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX2986847.1"
                     /translation="MAKNKCGCGCGGDGANGGSDIKEFVKEKYGKVAVNASERKETSC
                     CGGGTSKDAITRDLYSEAETQGIPVEALLASMGCANPLALARLNPGEVVLDLGSGGGI
                     DVLLSAKRVGPAGKAYGLDMTDEMLDLARKNQAEAGIENVEFLKGEIEDIPLPDESVD
                     VIISNCVINLSPDKDQVLREAFRVLKPGGRFAVADIVLTRPVSDEVKEHVGLWTGCIA
                     GALEMNDYKIKLLRAGFTDADVEVVKTYSVEDADGLVPEEMAAQLGAERTAELMAAFV
                     SAFVRATKPERSCCCG"
     gene            complement(3554..4624)
                     /gene="arsB"
                     /locus_tag="VHS06_01575"
     CDS             complement(3554..4624)
                     /gene="arsB"
                     /locus_tag="VHS06_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX2986848.1"
                     /translation="MGEGITKRLSFLDRYLTLWIFLAMGLGVGAGYSVPGAADFWNRF
                     AVGTTNLPIAIGLILMMYPPLAKVKYEELGEVFRNWRVLALSLVQNWVVGPILMFVLA
                     IIFLRDYPEFMVGLIMIGLARCIAMVIVWNELAKGDTEYAAGLVAFNSIFQVLFFSVY
                     AYVFITVLPPLFGLKGSTVHVTIGEIAKSVFIYLGIPFLAGILSRVVGLKTMGREWYE
                     KRFIPRISPITLIALLFTIIVMFSLKGEYIVKLPLDVVRIAIPLLIYFVVMFMVSFYM
                     GRRIGAGYPKTATLSFTAASNNFELAIAVAVAVFGINSGQAFAAVIGPLVEVPVLISL
                     VNVALRFRAEYFAKETGEVNYG"
     gene            complement(4626..5054)
                     /locus_tag="VHS06_01580"
     CDS             complement(4626..5054)
                     /locus_tag="VHS06_01580"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX2986849.1"
                     /translation="MKKVIFVCIHNSGRSQMAEAFAKRLGEGRILAESAGTMPGGALN
                     PQAVAAMEEIGYDMSGHYPKVMTPEMVASADKVITMGCGVDLGDIDHGEAVCPAVLVE
                     SEDWGLEDPKGQPIEKVREIRDQIKVRVERLIEEMTTSEG"
     gene            complement(5105..6094)
                     /locus_tag="VHS06_01585"
     CDS             complement(5105..6094)
                     /locus_tag="VHS06_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX2986850.1"
                     /translation="MLLCVSWYSLYRSLQPAADWFAYGLLGLSKASHIGSAVAFFVFE
                     VPKVLMLLALVVFGVGIIRTFFTPERTRLILAGRSEVLGNVLAGLLGIVTPFCSCSAV
                     PLFIGFVQAGIPLGVTLSFLVAAPMINEVAVVLLFGMFGWKVAAIYVTTGLVIAIVAG
                     WVIGRLRLERFVEEWVYEFQSGADGYEAEELTWSERVESGRAAVREIVGKVWPYVVAG
                     IAVGAGIHGYVPEGLMASFMGKSAWWAVPAAVALGVPMYSNAAGIVPVIHALLEKGAA
                     LGTALAFMMAVIGLSLPETIILRKVLKMQLIAVFVGVVAFGILIVGYLFNLIF"
     gene            complement(6213..6521)
                     /locus_tag="VHS06_01590"
     CDS             complement(6213..6521)
                     /locus_tag="VHS06_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006287804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2986851.1"
                     /translation="MDEVFKALGDPTRLKIVRMLAENGETCVCRIVDELGMNQPAISH
                     HMAKLKQAGLLTARKEGQWIHYSLKLEALKDGPLAFLAEIVSLAEASSNAMSGVSCCE
                     "
     gene            complement(6599..7297)
                     /locus_tag="VHS06_01595"
     CDS             complement(6599..7297)
                     /locus_tag="VHS06_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX2986852.1"
                     /translation="MEQLFIALTNAIEGAAPAALGASFVWGILSVVLSPCHLSSIPLI
                     IGFISDQGKLSTRRAFGLSFLFGLGILVTIALIGVVTAAMGRMMGDVGRYGNYAVAAV
                     FFAVGLHLIGVIPMPFSGPGKVGIKRKGLAASFALGLVFGIALGPCTFAFMAPVLTLS
                     LKVAATNAAYAMLLLAVYGVGHCAVIVAAGTSTEAVQRYLNWNEKSKGVGIVKAIFGV
                     LVMLGGVYLIRSAN"
     gene            complement(7299..7805)
                     /locus_tag="VHS06_01600"
     CDS             complement(7299..7805)
                     /locus_tag="VHS06_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2986853.1"
                     /translation="MRRSWVRLLAVGSLIAVIGMGASVQGAGKRPVSKVKTGAVCAKP
                     APKKAPTKAPKAAAKPSAKKNDPKAKAAASAPKTLPRLVDLGATKCIPCKMMVPVLDE
                     ISKEYKGKLKVEFVDVWKDEAAARKYNVQNIPVQIFFDAKGKEVYRHVGYFPKDGIVA
                     KLAEMGVK"
     gene            complement(7854..8099)
                     /locus_tag="VHS06_01605"
     CDS             complement(7854..8099)
                     /locus_tag="VHS06_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2986854.1"
                     /translation="MKIQILGTGCAKCKALTENAKKAVELAGVDAEIEKVEDIREIMK
                     FNVLMTPGLVIDGEVKSAGKVLSPDDIKKLILAAGGR"
     gene            complement(8152..>8500)
                     /locus_tag="VHS06_01610"
     CDS             complement(8152..>8500)
                     /locus_tag="VHS06_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986855.1"
                     /translation="TNDFAGLAEREGKTDTEIYEELRDGVGQISGLLGLDSTVPGMFW
                     KYGSLACDMAYNLTELRLGWKNVSALEENNTRYAEAVKKLAARMRELEERAKELRRKI
                     EAGEPTDFSASAK"
BASE COUNT         1716 a         2928 c         2213 g         1643 t
ORIGIN      
        1 gacgggtaaa gctgatgggt ggcgatatcg acatacggcc cgtaggcgtc gagatggaac
       61 gggttgtgcc ccgctaccgg gtcgaccgag atcaccctca tctcaggcgc aagtctgcgg
      121 gtcatccgga aggtctcaat ctgccagttg aggatctgct tgttcaccca cttgtggtag
      181 gcgatccagc gatacgggtt ctcatcggta agtgacgtcg gaattccata cttgccgaag
      241 ccgaactccc tcttgacctg ctcgtcgacc ttgcggatgt agggataggt gtccttcatc
      301 tgctggaaga acaacacccc ctgccttgtc gccctctcgt gtacctcgtc ctgggtgtat
      361 atcccccata tatacttgtg gtaggtctcg atggccgtct tcaggtcgtg gaagtaggcc
      421 gccttgctcc gaggatcgag cacatacggc agcccttcga tgacaggaac cccctccttc
      481 ttcgaagacc ggtaatccgg ctgatacagg accatgaacc tgtatctgtc ggccatcttc
      541 ctgtagttct catcctgcag gttcccccgc tggcagatcg gcaggagcct ccgctgcgta
      601 agctccttga actgctcctc cagcgcatac cttatcccgt gctccttggc aaagggacgg
      661 agattggagg cattgccgct gtggaaccag tagatggccc cgttcctctg atccctgccc
      721 gccgacgagg tcttcgacag cagctcctca aacagcgggt ccttcacatc ccacaccctc
      781 gacgcactcg gggtggggcc ggccatcgct ccgagggcgg agaccagcac gaacagcact
      841 aatgcggtac aacggacgac gatcttcatt gagcattcac tcctgtcgat gacggtcgaa
      901 ccacgccgat aataccgact acatccgtgg aagcgggttc tgcctgtggc actcctcgat
      961 taccgctatc tgctgacgga cctgctccgg gggaatcaca agcggagcgc cgttcactat
     1021 ggtctcgtaa aggtgggtgt agaaggtcac cgccatatac tggaaccagt cggaattctc
     1081 ctgaggaagg tcccagcttc cctcgtgcca ctcgagcttc tcggagcagt aagccctgtc
     1141 cggcagcggc tcgcggcaca gatgctgctg cggcgcctcc tcaggcttga agtacttcca
     1201 ctccaggtga ttcgccgtcc ccttcagccc gccaagggtg ccgtcgatgt tgtacatatg
     1261 ggtcggatag gcgcagcatg aggatatctc cacatcgatc accggccgcc ccttcccctt
     1321 caggatgatc ttgacgaagt cctcggcgtc cccgaagcag ttcgcccggt ccatgatgca
     1381 ggtgacctcg ggcataacgt cagtcccgaa gagctgaagc gcctggtcca tcggatgagg
     1441 cccggtgttc agcagattcc ccgcgttgtg gtcctgcagc gtctgccagt cccaccttct
     1501 accgaacccg ttgaacgcga tcctgaccat cacgatccgc cccagcaccc cggactcgat
     1561 cacttccctg atcttccgga aatacggagc aaaccgcgac tgctgataga tcgcaaagag
     1621 cttccccgtc tccttcgact tcgctataag ctgatcgacc tccgacgcgt acctggcaag
     1681 cggcttctcg cagagcacat tgtgccccga gtccatgatc tccatacaca ccggcacgtg
     1741 cagatggctc ggagtagcat tgaccaccag gtcgaggtca tccctcttca acatctcccg
     1801 atagtccctg tatcccgcgg cccccaactg ctccaccgca tcatcacacc gcccgtccaa
     1861 cggatccgcc acggcgacga cctgatacct gtcagtcatc tcccgcagcc cgcgcacgtg
     1921 gatgctataa ccactccgcc cctgcccaat cacaccaact ctgacgactt tgctcatttc
     1981 cttgacctct tcccgctcaa ctagagaatc cggcgccaca gatgaacacc cgcgctacga
     2041 atctccacct tctgcccaga aagcacaaat ccctggagtc ccacggtccc cagaagtgcc
     2101 tggtcaggag accttggcac agcagagact acccgaacat tatgtccgct tgtcgctagg
     2161 tcgtcaaggc ttaagaggcg gatcaacacg ccttaccgct cgacaccact gccccacggc
     2221 gtcatccggc cccacgccga ccaggtcaac cgcgcgctcg tgggctctct cagggggcga
     2281 tagatcagag gggccgctat cgacatcacc gccggggagc cggcacagca tcgtccctcc
     2341 cccgcccttt accaggtgcg ggaagggtga aggcgatcag ggcgcagagg acggggagag
     2401 cggccacaca taggagtgcc gactggacac cgtgaaggtc ggcccatcgg cccaggaacg
     2461 gggcgacgat cccgccgacg ctcaccgcga gccccagtgt cacccccgag gcgaatccca
     2521 cacggttggg caggtactgc tggcccagca cgacgatggc gctgaacgga gcgtagtaca
     2581 tcaagcccag gggtatcagg ccgatagcgg ccatcgtgtg gcaggcgttg ttaccgacac
     2641 cgaccacacg tggcctctgc atgcaatcac gccccgccgt ccctacccgc agcagcagga
     2701 gcgctcgggc ttggtggctc tgacgaacgc gctcacgaaa gccgccatca gctcagcggt
     2761 cctttccgcg ccgagctgcg cagccatttc ctccggcacc agcccgtcgg cgtcctcgac
     2821 cgagtaggtc ttgaccacct cgacgtcggc atcggtgaaa ccggcccgca gcagcttgat
     2881 cttgtagtcg ttcatctcaa gggccccggc aatacatccc gtccacaagc cgacgtgctc
     2941 cttgacctcg tccgagaccg gtctggtgag aacgatgtcg gccacggcga acctgccgcc
     3001 gggtttgagc acccggaacg cctcgcgaag cacctggtcc ttatcaggcg agagattgat
     3061 gacgcagttc gagatgatca catcgaccga ttcgtccggc agagggatgt cctctatctc
     3121 gcccttcagg aactcgacgt tctcgatccc cgcctccgcc tggttctttc gcgccaggtc
     3181 cagcatctcg tcggtcatat ccaggccgta cgccttaccg gccggtccga ctcgcttggc
     3241 ggacagcagg acgtcgatgc cgcccccgct ccccaggtcg agcaccacct caccagggtt
     3301 cagcctcgcc agtgccaaag ggttggcgca gcccatcgag gccaggagcg cctcgaccgg
     3361 tatcccctgc gtctcggcct cagagtagag gtcgcgggtg atcgcatcct tgctggtacc
     3421 gccgccgcag cagctcgttt ccttccgctc ggaagcgttg accgcaacct taccgtactt
     3481 ctccttgacg aactccttga tgtcgctgcc gccgttggct ccatcgccgc cgcagccgca
     3541 tccacacttg ttcttagcca tagttgacct cacctgtctc ctttgcgaag tattctgccc
     3601 ggaagcgaag cgctacgttc accaggctga tcagcaccgg cacttccacc agtggaccga
     3661 tcaccgccgc aaacgcctgc ccggagttaa tgccgaacac agccaccgcc acagctatcg
     3721 ccagctcgaa gttgttgctc gccgccgtaa acgacagcgt tgcggtcttg ggatatcccg
     3781 cgccgatcct cctgcccata tagaagctca ccatgaacat caccacgaag tagatcagca
     3841 gcgggatggc tatccttacg acgtcaaggg gcagcttgac gatatactcg cccttcagcg
     3901 agaacatcac tatgatcgtg aaaaggaggg cgatcagcgt tatcgggctg atccgcggga
     3961 tgaagcgttt ctcataccac tcgcgtccca tcgtcttgag cccgacaacc ctgctaagta
     4021 tcccggcaag gaacggaatc cccagataga tgaacacgct ttttgctatc tcgccgatgg
     4081 tcacatggac agtgcttccc ttcagcccga agagcggcgg aagcaccgtg atgaacacgt
     4141 aggcgtagac cgagaagaag aggacctgga agatcgagtt gaacgccacc agccccgcag
     4201 catattcggt gtcccccttg gcgagctcgt tccagacgat gaccatggcg atgcaccgcg
     4261 ccagcccgat catgatcagc ccgaccatga actccgggta gtcgcgcagg aagatgatcg
     4321 ccagaacgaa catcaggatc ggcccgacga cccagttctg cactagcgag agcgccagca
     4381 cccgccagtt gcggaacacc tcgccaagct cctcatactt caccttcgca agcggcggat
     4441 acatcatcag gataagcccg atggcaatcg gcaggttggt cgtgccgacg gcaaagcggt
     4501 tccagaagtc ggcagcgccg ggtacggagt agcccgctcc caccccaagg cccatcgcca
     4561 ggaatatcca cagcgtcagg tacctgtcca gaaatgatag tctcttcgta attccttcgc
     4621 ccatgctaac cctcgctcgt agtcatctcc tcgatcagtc tctccaccct aacctttatc
     4681 tggtcccgga tctcccggac cttctctatc ggctggccct tcgggtcctc gagcccccag
     4741 tcctcggact ccaccagcac tgccggacag accgcctcgc cgtggtcgat gtcacccagg
     4801 tccacgccgc agcccatcgt aataactttg tccgcactcg cgaccatctc cggagtcatc
     4861 accttggggt aatggccgct catgtcgtag ccgatctctt ccatcgccgc cacggcctga
     4921 ggattgagag cgccgccggg cattgtaccg gccgattccg ccagaatcct cccctcgcca
     4981 agcctctttg cgaacgcctc agccatctgg ctccggcccg agttgtgtat gcaaacgaat
     5041 atgaccttct tcaacttcct acttctccca caaagaacgc gtagtacagc cgtgccacca
     5101 gagatcagaa gatcagattg aacaggtagc cgactatcag gatgccgaag gcgacgacac
     5161 ctacgaaaac agctatcaac tgcatcttca gcaccttacg aaggatgatc gtctccggca
     5221 gagacaggcc gataacggcc atcataaagg ccagcgccgt ccccagagcg gcgcccttct
     5281 ccagcagcgc atggataacc ggcactatcc ccgccgcatt ggaatacatc gggactccaa
     5341 gcgcgaccgc ggcgggcacc gcccaccacg cgctcttgcc catgaacgag gccatcagcc
     5401 cctccggcac gtagccgtgg attccggccc cgacggcgat accggccacc acatacggcc
     5461 agaccttgcc gactatctcg cggaccgccg cccttccaga ctcgaccctc tcagaccagg
     5521 tcagctcttc agcctcgtaa ccgtccgcgc ccgactggaa ctcatagacc cactcctcga
     5581 cgaatcgctc gagcctcagc ctgccgatca cccatccagc gactatggcg atgaccagcc
     5641 cagtcgtcac gtatatcgcc gccaccttcc agccgaacat cccgaagagg aggactaccg
     5701 cgacctcgtt tatcatcggc gcggcgacca aaaacgagag cgtcacccca agcggtatcc
     5761 ccgcctgcac aaacccgatg aagagaggca cagccgaaca ggagcagaac ggggtaacga
     5821 tcccgagcag ccccgccagc acgttgccga gcacctcgct gcgccccgcc agtatcagtc
     5881 tcgtcctttc aggggtgaag aacgtccgga tgatcccgac accgaagact accagcgcga
     5941 gcagcatcag gaccttgggg acctcgaaca cgaagaaagc caccgcgctt ccgatgtggc
     6001 tggccttcga aagccccagc aacccgtatg cgaaccagtc agccgcaggc tgcagcgatc
     6061 tgtagagtga gtaccaggag acgcacagca gcacgaaggg gagcgccctc ctccaaagcg
     6121 gcgtccgtat ctcagcggta tcgcgggcag caacggtgtt ccgctctaca tccactttcg
     6181 gcccgcccct ttctgctctg tcaccgttcc gatcactcac aacaggaaac cccgctcatc
     6241 gcgtttgaag atgcctcggc aagcgagact atctccgcta gaaaggccaa cggcccatcc
     6301 ttgagcgcct cgagcttgag cgagtagtgt atccactgcc cctccttgcg cgctgtcaga
     6361 aggcccgcct gtttcagctt cgccatatga tgcgatatcg caggctggtt catccccagc
     6421 tcgtccacga tcctgcagac gcacgtctcg ccgttctccg caagcatccg aactatcttg
     6481 agcctggtcg gatcaccaag cgccttgaac acctcgtcca tagctcacct atcagtatat
     6541 gtgtatatac tgatatacta gcaccaacca aacatctccg tcaacacttc tgttggaatc
     6601 agttcgcact ccggatcaga tagaccccgc cgagcataac cagcacgccg aagatcgcct
     6661 tgacgatgcc gacccccttc gacttctcat tccagttcag ataacgctgg actgcttccg
     6721 tcgaggtgcc ggctgccacg ataaccgcgc agtgccctac cccgtagacc gcgaggagca
     6781 gcatggcata cgcggcgttg gtcgccgcaa ctttcagtga gagggtcagc accggagcca
     6841 taaaagcgaa cgtgcacgga ccaagggcga tgccgaacac cagccccagg gcaaaggacg
     6901 cggcaagccc ctttcgcttg atcccgacct tgcccggacc gctgaacggc atcggtatca
     6961 caccgatcag gtgcagaccg acagcgaaga agacggcggc cacggcgtag ttaccgtatc
     7021 tgccgacgtc tcccatcatc cgccccatag ccgcggtcac aacacctatc agcgcgatgg
     7081 tgacgagtat gccgagtccg aagagaaacg agagcccgaa cgcccgccgc gtactcagct
     7141 ttccctggtc gctgatgaag ccgatgatca gcgggatgct cgacagatgg cacgggctca
     7201 ggacgacgct cagtatcccc cagacaaacg aagcccccag cgcggcgggt gctgctccct
     7261 cgatcgcgtt ggtgagtgct atgaagagct gctccagact acttgactcc catctccgcc
     7321 agctttgcga ctatcccatc cttcgggaag tagccgacgt ggcggtagac ttccttgccc
     7381 ttggcgtcga agaagatctg aaccgggatg ttctggacat tgtacttccg tgcggcggcc
     7441 tcgtccttcc agacgtcgac gaactcgacc ttgagcttgc ccttatactc cttggagatc
     7501 tcgtcgagga cgggaaccat catcttgcag gggatgcact tcgtcgcccc caggtcaacc
     7561 agcctcggaa gagtcttggg agcgcttgcc gcagccttcg ctttcggatc gttcttcttc
     7621 gccgacggct ttgcggcggc cttaggtgcc ttagtcggcg ccttcttcgg agccggtttc
     7681 gcgcacacag cccctgtctt caccttcgac acaggtctct tccccgcacc ctggaccgac
     7741 gcgcccatcc cgataacggc gatcagcgat ccgaccgcca acagtctgac ccaacttctc
     7801 ctcaccacat cctccttctg cgtgaacgcc tccgttcaag gcggcgtatc ctcctagcgg
     7861 cctccggcag ccagtatgag cttcttgata tcgtcgggag acagcacctt cccggccgac
     7921 ttgacctcgc catcgatcac cagccccgga gtcataagga cgttgaactt catgatctcc
     7981 ctgatgtctt cgaccttctc gatctccgca tccactcccg caagctcaac cgccttcttg
     8041 gcgttctccg tcagcgcctt gcacttggcg cagccggtac ctagaatctg gatcttcata
     8101 tcggacctcc atcgtctgaa acgcactgcc gtttccgagc agtcgatgct gttacttggc
     8161 tgaagcgctg aagtccgtag gttcgcccgc ctcgatcttg cggcggagtt cctttgcccg
     8221 ttcctccagc tcccgcatcc tggcggcaag cttcttgacg gcttcggcgt atctggtgtt
     8281 gttctcctcc agagcactca cgttcttcca acctagtcgc agctccgtca ggttgtaagc
     8341 catgtcacac gccagtgagc cgtatttcca gaacatcccc ggaacggtcg agtccaaccc
     8401 gagcaagccg gatatctggc cgaccccgtc cctcagctcc tcatatatct ccgtatcggt
     8461 cttgccctcg cgttcggcga ggccggcgaa gtcgttggtc
//
LOCUS       DATALS010000078         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_273468, whole genome shotgun sequence.
ACCESSION   DATALS010000078 DATALS010000000
VERSION     DATALS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273468"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VHS06_01615"
     CDS             complement(<1..167)
                     /locus_tag="VHS06_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX2986856.1"
                     /translation="MKALILAAGEGTRLRPLTLTCPKPMLPVGEIPLLAHIFRWIRGQ
                     GVRDVAINLHYL"
     gene            complement(273..1436)
                     /gene="larC"
                     /locus_tag="VHS06_01620"
     CDS             complement(273..1436)
                     /gene="larC"
                     /locus_tag="VHS06_01620"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX2986857.1"
                     /translation="MIAYFDCYSGISGDMTLGALVDAGVDPEALKAEVAKLGLDGYEL
                     RFERMERHGITGTHAAVDLDPSIPQPHRHLSDILAILDGSALAPSVKQRASDVFRTLG
                     EAEASVHGVPVDHVHFHEVGAVDALVDVVGSVAGLELLGVKEVFCSVLPSGKGTIEAA
                     HGILPVPAPATLEIMARAGIPTKSVDVEAELVTPTGAAIVATLATFKQPDVRVRSVGY
                     GFGTKQLPWANALRLWVGEPLEEGASTEETSLIEANLDDMPAEALGYAMERLFAAGAL
                     DVYFTPIQMKKNRPATMLSVISSLDKEQEIASMVLRETTTLGVRVSRTSRVTAERRVE
                     TVDTTWGPVRVKVKSFGGITSYSPEFEDCARVARERDLPLQAVVQGVLDVCPR"
     gene            complement(1505..>3011)
                     /locus_tag="VHS06_01625"
     CDS             complement(1505..>3011)
                     /locus_tag="VHS06_01625"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX2986858.1"
                     /translation="EETVKRQDGGDGLQRPRRRQGTGVWQSFSPDGTIHRLDPMHRRT
                     VSSDTTVRHRKFQTSMDCQKSVMDYQEALDWIMSYWEPGRPKSQEKALRPLKVPRMRV
                     LLSRLGSPQLAFPSILVAGTKGKGSTAAFIAEGLRAAGYRVGRYTQPHLIDWRERTWV
                     DGRIIEPDEVARLAERIRPVVDDLNRDMVDLGTITTYEVGTALTLCYFAERKVDVAVL
                     EIGVGGRLDALNAVEPILSAITSISLDHTDVLGDSLDEIATEKAGILRYRRPAVVASQ
                     RPEAEDAIRRVATEKGAHLSTVGRDWRWVEGTDAGTVDIFGPRGELKGLRVALLGDHQ
                     RDNATTAVAALQLLREQGFGVGDDAIRRGLAEVEWPGRIQRLRRSPYLVADAAHNVDS
                     VSRLLETVRKEFSFVRLILVFGASGDKNVEGMARVLGPAASRVVVTSSGHRRAADVER
                     LAGVFAHFGEVVAEPDPEAAFEVALGLAAPEDLVLVTGSVFLAGRAVAVFQ"
BASE COUNT          550 a         1094 c          861 g          506 t
ORIGIN      
        1 agatagtgga ggtttatcgc cacatcccga acaccctgtc ccctaatcca gcggaagatg
       61 tgggcaagga gcgggatttc ccccacgggc agcattggct tgggacaggt aagagtgagg
      121 ggccgcagcc tggtgccctc acccgcggcc aggatcagcg ccttcatctc caacacctaa
      181 gaggggatgg cgaaccaacc gtctccgggg cgcgtgatgc tcttggtaag gcaatggtac
      241 agcctacggc aagccacggg caactgcgaa ggctacctcg ggcagacatc gagcacaccc
      301 tgaacgaccg cctgcaacgg caaatccctc tccctggcca cccgggcgca gtcctcgaac
      361 tctggagagt agctggtgat gccgccgaag ctcttcacct tgacccgaac cggtccccag
      421 gtggtatcca cagtctcgac ccgtcgctcc gcggtgaccc tgctggtgcg gctgacccga
      481 acgccgaggg tcgtggtttc gcgcagcacc atggaggcaa tctcctgctc cttgtcaagg
      541 gaggagatca cgctgagcat cgtcgcgggg cggttcttct tcatctgaat cggggtgaag
      601 tagacgtcca gggcacccgc cgcgaacagc cgctccatgg cgtatcccag cgcctcggcg
      661 ggcatgtcgt ccaggttcgc ctcgatcagc gaggtctcct cggtggatgc cccctcctcc
      721 aatggctcgc cgacccatag ccgcaacgca ttggcccagg gaagttgctt cgtcccgaag
      781 ccgtagccca cggagcgcac ccggacgtcg ggctgcttga aggttgcaag ggtggcgacg
      841 atagcggctc ccgtcggggt caccagttcg gcctccacgt cgaccgactt agtggggatt
      901 ccggcccgag ccatgatctc cagggtggcg ggggctggca ccggcaggat cccgtgagcc
      961 gcctcgatgg tccccttgcc ggaggggagc accgagcaga agacctcctt cacacccagc
     1021 agctccagcc ccgctaccga gcccactacg tccaccagcg cgtccaccgc gcccacctcg
     1081 tggaagtgta cgtggtccac cggcacgccg tgcaccgatg cctcggcctc gccaagggtt
     1141 cggaagacgt ccgatgcccg ctgctttacc gacggcgcca gggcagagcc gtccaggatg
     1201 gccaggatgt cggaaaggtg gcggtggggc tgcgggatcg agggatcgag atcgaccgct
     1261 gcgtgggtgc cggttatccc gtgccgctcc atgcgctcga agcgcagctc gtacccgtcc
     1321 agccccagtt tggcgacctc ggccttcaga gcctccggat cgacgccggc atccaccagt
     1381 gcccccaggg tcatgtcccc gctgatcccg ctgtaacaat cgaagtacgc tatcatctct
     1441 ccctcaccat cgaacacatt tccatcgcca tccataagct gtagggtggg ttgtctcacc
     1501 ccacctattg gaacaccgcc actgcccgcc ccgcaaggaa caccgacccc gtcaccagca
     1561 ccagatcctc cggcgcggca agcccaagcg ctacctcgaa ggctgcctcc ggatcgggct
     1621 ccgctacgac ttcgccgaag tgggcgaaca cgccggcaag ccgctcgacg tccgccgccc
     1681 gccgatggcc ggaactggtc accaccaccc gcgatgccgc cggtcccagc acacgggcca
     1741 tgccttcgac gttcttatcg ccactcgcgc cgaagaccag gatcaaccga acgaagctga
     1801 actccttgcg aaccgtctcc agcaggcggc tcaccgaatc cacgttgtga gccgcgtcgg
     1861 caacgagata cgggctccgg cgcaaccgtt ggatccttcc aggccactcc acctcggcca
     1921 ggccacgacg gatagcatcg tcacccacgc cgaacccctg ctctcgcaac agctgcagtg
     1981 cagccacggc cgtcgtcgca ttgtcgcgct gatgatcccc cagcagcgcc actcgaagcc
     2041 ctttcaactc gccacgggga ccgaagatgt cgacggtgcc ggcatccgtc ccctccaccc
     2101 agcgccagtc cctgccgaca gtggagagat gagccccctt ctccgtcgca acccgccgaa
     2161 tcgcgtcctc ggcctccggc cgctgcgacg cgaccaccgc aggccgccta taccgaagga
     2221 tgcccgcctt ctccgtcgcg atctcgtcca ggctgtcccc cagcacgtcg gtgtggtcca
     2281 ggctgatcga ggtgatggcc gacaatattg gctcgacggc attcaaggca tccagcctac
     2341 cgccgacacc gatctccaac acggccacgt ccaccttgcg ctcggcgaaa tagcataggg
     2401 tcaacgccgt ccccacctcg taggtggtga tcgtgcccag atccaccatg tcacggttga
     2461 gatcatccac caccggccgg atccgctccg ccaggcgggc aacctcgtcg ggctcgatga
     2521 tccggccatc gacccacgtc cgctcccgcc agtcgatcag gtgaggctgg gtgtaacgcc
     2581 caaccctgta acccgcggct cggagtccct cggcgatgaa ggccgcggtg gaccccttcc
     2641 ccttggtccc agccaccagg atcgacggga aggccagctg aggagacccc aatcgggaca
     2701 gtaacacccg catccggggt accttgagcg gcctcagcgc cttctcctgc gacttcggcc
     2761 tccccggctc ccagtatgac attatccagt cgagcgcttc ctgataatcc atcacagatt
     2821 tctgacaatc cattgaagtt tgaaactttc gatgccgcac ggtggtgtcc gatgaaacag
     2881 tgcgccgatg catcggatcc agccggtgga tggtaccatc cggcgaaaag ctttgccaca
     2941 cccctgtgcc ttgccgtcgc ctcggccgct gcaacccatc gccgccgtcc tgccgcttaa
     3001 cagtctcttc c
//
LOCUS       DATALS010000079         8285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_6857,
            whole genome shotgun sequence.
ACCESSION   DATALS010000079 DATALS010000000
VERSION     DATALS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6857"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VHS06_01630"
     CDS             <1..924
                     /locus_tag="VHS06_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX2986859.1"
                     /translation="HTYFVDPQERNISETQLQGGFEGIGVTVEMTKDGKVRVVAPQEG
                     SPGEKAGIQPGDLITHVDGKSLAGLTLTQAVSLIRGPKDTTVTLTIARENAPDPLTFE
                     IARAEIRVESVRAEMLDQNVAYIRISQFGTQTSGELKTALQQLLDQNPQGLILDLRAN
                     PGGYLVAAVDVASQFLKDGIVLVEERSGGERQAYRVKQGGIATDIKMVILVDKGSASA
                     SEIVAGALQDYERATLIGTTTYGKGSMQSVHTLSDGSSIHVTIAHWLTPKERPINGNG
                     LVPDVEVQYPNDPVAEGDPQLDKAIEVLVGQ"
     gene            972..1439
                     /gene="smpB"
                     /locus_tag="VHS06_01635"
     CDS             972..1439
                     /gene="smpB"
                     /locus_tag="VHS06_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035202732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX2986860.1"
                     /translation="MPERTERVYANNRKASHDYDILETVEAGMVLTGTEVKSVREAKI
                     SLRDSYARIKDGEVWMYNSYIATYDQGNRFNHETGRPRKLLLHKGEISRIAGLVQDVG
                     KTLVPLRVYDRKGHIKVSLGVARGKKLYDKRESLARREAGREVERALKEAVRG"
     gene            1482..1837
                     /gene="ssrA"
                     /locus_tag="VHS06_01640"
     tmRNA           1482..1837
                     /gene="ssrA"
                     /locus_tag="VHS06_01640"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1900..3348
                     /gene="guaB"
                     /locus_tag="VHS06_01645"
     CDS             1900..3348
                     /gene="guaB"
                     /locus_tag="VHS06_01645"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX2986861.1"
                     /translation="MEKIIAEGLTFDDVLLVPRYSELLPSNVSVQTRLTKRIALHIPV
                     LSAAMDTVTESRMAVALAREGGMGIIHRAMTIERQAEEVDKVKRSESGMITKPVTLTP
                     SNTLADAWRMMEQYHISGVPIVDQEMRLVGILTNRDIRFETGGEKRIADLMTSRGLIT
                     APVGTTLEEAKDILHKNKIEKLPIVDEDGILKGLITVKDIQKKINYPHAAKDSLGRLL
                     AGAAVGVGADLVDRASALVEAGADILSIDAAHGASKNVVRALTTLKQRFPNMEVIAGN
                     VATYDGAKLLMDAGADAIKVGVGPGSICTTRVVSGAGVPQITAVMEAWRATQGEVPLI
                     ADGGIKYSGDITKALCAGADVIMLGNLLAGVEESPGEVVVYQGDRYKEYRGMGSLGAM
                     KYFGRDRYGLSEGEKLVPEGVEGRVPYKGLLSNVLYQLVGGLRSGMGMLGCGTIPELQ
                     ENGRMIRITQASLTESHAHDIYLTREAPNYSR"
     gene            complement(3526..3801)
                     /locus_tag="VHS06_01650"
     CDS             complement(3526..3801)
                     /locus_tag="VHS06_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986862.1"
                     /translation="MAEKPKESEEKRTPKPEERHVQFDQNSWTEIDAEGTDSEMAQGR
                     ESQEPFYTPLVTEAAERKMKKDREAREAQRRQEKEQKERGEGKERAA"
     gene            4225..4500
                     /locus_tag="VHS06_01655"
     CDS             4225..4500
                     /locus_tag="VHS06_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2986863.1"
                     /translation="MTALGQKVKELRVSKGMTQQRLAEKAEVSHSLISALESGRRKYV
                     SAQDIERLAKALDVPANDFWKLVPGGKSEQRYIPITGTGRSEMAKSA"
     gene            complement(4562..6319)
                     /gene="mutL"
                     /locus_tag="VHS06_01660"
     CDS             complement(4562..6319)
                     /gene="mutL"
                     /locus_tag="VHS06_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX2986864.1"
                     /translation="MGIHLLSADTVGKIAAGEVVERPASVVKELLENSLDAGARTIHL
                     EIAGGGTDLIRIADDGCGIPRDELPLALERHATSKISSAEELSSISTLGFRGEALASI
                     AAVSELTVLSRTAGEASAFQLRSNGGVTGPATVSARAVGTTISVERLFYNVPARRKFL
                     RTNAAEAAQVAQLVSQYAIAYPEVAFQLQIEGRKSLTTPGSGNPLDAAMQVLGKDVAG
                     FLLPVEASTEVDHAQGRTAIRVSGYVADPGVSRASRSSIWLFVNRRFIKSHSLSFAVE
                     ESYQTLLQVGRHPIAFVNLQVPPTEVDVNVHPTKTEVRLLRERAIYATLRDAVRETLA
                     GGSSWAQQVSGLEAPQEKIAPWETIPPTSPPTPGQSPVSMPRLIDTAITANAAAPAAP
                     VGGHRLPILRLMGQVAQSYIVAEGEQGLYLIDQHAAHERVLLERLHQSMDREGNTQLL
                     LEPIVLEVTALQREIAEAAGSALESLGFRLEPFGEQSILVRGIPSELPSSRAVQALEQ
                     SLQELTDNAGEADWRERMAVALSCRSAVKAGQPLSAEEMRALVEALEEAGINQHCSHG
                     RPTAILLSRSQLEREFGRR"
     gene            complement(6319..>8285)
                     /gene="mutS"
                     /locus_tag="VHS06_01665"
     CDS             complement(6319..>8285)
                     /gene="mutS"
                     /locus_tag="VHS06_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX2986865.1"
                     /translation="FCLTPGSAATRAAVRLLVYLQDTQPASLGQLESLQVYSLDRYMK
                     LDSAARHHLEITANAVTGNVEGSLLGVLDRTLTPMGGRLMRRWVGQPLLDLDSVEARL
                     NAVQELVSSPLGRADLSKNLAGLGDLERLASRAAQQGVSPRDCIAIARSLRVVPRLLQ
                     GLKQWLPGILAAESAALDACPEAIDAISSTIADEPAVVVGQGVIREGKSQDLDDLRGI
                     SGDTRKWIAGLEHGERERTGVKSLKIGYNKVFGYYIEVTRPNLDAPTDEFARVKTGAG
                     TVGEMLETMGYQRKQTLANAERFVTVQLKEQEVRLASAQEEIEALEKRIYAELVTELA
                     GLAGRIRRTAQSIARVDALLSLAEVAVANRYVRPTVDDGSVISIREGRHPIVERTISI
                     GEYVSNDCFLDSDSSQIVVLTGPNMAGKSTYVLGVALIVLMAQMGSFVPAEEAHIGLV
                     DRVFTRVGAQHDVTGGKSTFLVEMAETANLLRNATPRSLVLLDEVGRGTSTYDGMAIA
                     RAVVEHLHNDPKLRCKTLFATHYHELTELESLLPRVKNQRVDVLEEGGRVVFLHRVVP
                     GGADRSYGIHVAQLAGVPIPVIRRARQILEELESAAATPGRAEPHQQDGQGHELAELV
                     ETIATMDLSNMTPLQALAKLDELQRKATEI"
BASE COUNT         1637 a         2495 c         2509 g         1644 t
ORIGIN      
        1 cacacctatt tcgtagatcc ccaggagcgc aacatcagcg agacacaact gcagggtggc
       61 ttcgagggaa ttggtgtcac cgtggagatg acgaaggacg ggaaggtcag ggttgtagcg
      121 ccccaggagg gttcgcccgg cgagaaagca ggaatccagc ctggagatct catcacacac
      181 gttgacggca agtctctggc cggtctgacc ttgacccagg cagtctccct aatccgaggt
      241 cccaaagaca caacggtcac gctcactatc gcaagagaga atgcacccga cccactcacc
      301 ttcgagatag ccagggctga gatccgggtg gagtccgttc gggccgaaat gctcgatcaa
      361 aacgtggcct acatacgaat atcacagttt ggcacacaaa ccagtggcga attgaagacg
      421 gcccttcagc aactgctcga tcagaaccct cagggtctaa tcctcgatct ccgggcgaat
      481 ccgggaggct atcttgtggc tgccgtcgac gtggccagtc aattcctcaa ggacggaatt
      541 gtattggtcg aggagcgaag cggaggtgag cggcaggcat atcgggtgaa acaaggcggc
      601 atcgcaaccg acatcaagat ggtgatcctt gtcgacaagg gctctgccag tgcgtcagag
      661 atcgtagcag gcgcactcca ggattacgag agagcaaccc tgatcggcac tacgacctat
      721 ggcaaaggct cgatgcaaag cgttcacacc ctcagcgatg gatcgagcat tcacgtgacg
      781 atagctcact ggcttactcc caaggaacgg cccatcaatg ggaatggtct ggtccccgac
      841 gtcgaggttc aataccccaa cgatccggtc gcagagggcg atcctcaact ggacaaggca
      901 atcgaggttc tcgtggggca atagcgtcac aaccctttgg agaggcggcc tcacatctgc
      961 tataatatgg tatgcctgaa agaacagaac gagtctacgc aaacaacaga aaagcctctc
     1021 acgactatga catcctcgag accgttgagg ccggaatggt tctcacgggc accgaggtaa
     1081 agtcggtgcg cgaagccaag atcagcctgc gcgatagcta cgccaggatc aaggatggcg
     1141 aggtctggat gtacaatagc tacatcgcca cctacgatca gggcaaccgt ttcaaccatg
     1201 agacggggcg tccgcgcaag ctgctgctcc acaagggaga gatcagccgg atcgcgggac
     1261 tcgttcagga cgttggcaag acactggtgc cgctacgagt atatgatcgc aaggggcaca
     1321 tcaaggtgtc gctgggggta gctcgcggca agaagctgta cgacaagcgg gaaagcctcg
     1381 ctcgtcggga ggccggaagg gaagtggagc gagccctcaa agaagctgtt cgtgggtaaa
     1441 tcccgcgcag cagatggcta gttcatcatt tccaatctta agggggcgtc aggtttcgac
     1501 aggggacgaa acacgagaat cgcaagccga gggtgccatc gcgcctcgtt aaaaggtggc
     1561 aacggcataa ctgccaacga accagtagct ctcgcagcgt aacaacgttt cgagcgtccg
     1621 tccgttctct tctcgatgga tcggagtcgg gcgtcactaa gtcgagatgc tgcagcccag
     1681 accgcctact aagggttgct caagattagc gggctggttc ttctgtaccc cgtcggtagg
     1741 gtgcttggga acgagattta actgccgaat aagcttgtag catattctcg gtggactcgc
     1801 tctggacggg gagttcgatt ctcccccgcc tccaccatct ctatccctgc cccttactca
     1861 cggcggggat tttctttagc ctgaagggga gacttgccca tggagaagat aatcgccgaa
     1921 ggcctcacct ttgacgacgt gttactggtc cccaggtact ctgaactgct tccctctaat
     1981 gtgtccgtgc agacccgact cacgaagcgc atcgccctcc acatcccggt cctctccgca
     2041 gccatggata ccgtcaccga gagccggatg gccgtcgcgc ttgctcgcga gggcgggatg
     2101 gggatcattc atcgggccat gaccatcgag cgtcaggccg aagaggtgga taaggtcaag
     2161 cgctccgaga gcggcatgat cacgaagccg gttacgctga cgcccagcaa cacactggcc
     2221 gatgcatggc ggatgatgga gcagtaccac atctccggcg tgccgatagt ggaccaggag
     2281 atgagactgg tggggatact caccaatcgc gatatccggt tcgagaccgg cggagagaaa
     2341 cggattgccg atctcatgac cagcaggggg ctgatcacgg ctccggttgg caccaccttg
     2401 gaagaggcga aggatatcct ccacaagaat aagatcgaga aactcccaat tgtggacgag
     2461 gacggcatcc tcaagggctt gataactgtc aaggacatcc agaagaagat caactacccg
     2521 catgctgcca aggactccct cgggcgactg ttggccggtg ccgccgttgg tgtgggtgcc
     2581 gatctggtag atcgcgcgtc ggcgttggtg gaggctgggg ctgatatcct ctccatagat
     2641 gccgcccacg gcgcatccaa gaatgtagtt cgagccttga ccacgttgaa gcagcgcttc
     2701 ccgaatatgg aggtgatagc cggcaacgtc gccacttacg acggcgccaa gctactgatg
     2761 gatgcaggtg ccgacgccat aaaggtgggt gtcggccccg gctcgatctg caccactcgc
     2821 gtggtctccg gcgccggtgt gccgcagatc acggctgtta tggaagcgtg gcgtgcgacc
     2881 cagggcgagg ttccgcttat agccgacggt gggatcaagt acagcggcga catcaccaag
     2941 gcgctatgtg caggtgccga cgtgatcatg ctggggaacc tgctggcagg cgtcgaggag
     3001 agtcccgggg aggtcgtggt ctaccaggga gaccggtaca aggagtaccg cgggatgggt
     3061 tccctgggcg ccatgaagta cttcggcagg gatcgatacg gtctgtcgga gggcgagaag
     3121 ctggttcctg agggtgtgga gggcagggtg ccctacaagg ggctcctttc caacgtcctt
     3181 taccaactgg ttggtggcct tcgctccgga atgggcatgc taggctgcgg gacgatcccc
     3241 gagttacagg agaacggccg catgatcagg attacgcagg cgagtctgac cgagagtcac
     3301 gcgcacgata tctacctgac tcgagaggcg ccgaactact cacgatagca ggatgtgctg
     3361 gcggtatctg tttgccatga cggcaggccg gattgtggat gccgcctctg gcgtgtcgta
     3421 gggcggggtc ttgtgccccg cccttttggt tgcccacaga caggttggtg gctgtgacca
     3481 cggtgtgggc ggcggggcag agcgccccgc cctacgacag gacccctacg ccgcgcgttc
     3541 cttgccctca cccctctcct tctgttcctt ctcctggcgc ctttgcgctt cgcgagcctc
     3601 gcggtccttc ttcatcttgc gctctgccgc ctcggtgacg aggggcgtgt agaagggctc
     3661 ctggctctct ctgccctgcg ccatctcgct gtcggtcccc tctgcgtcga tctcagtcca
     3721 agagttctga tcgaactgga cgtgccgctc ctccggcttc ggagtgcgct tctcctcact
     3781 ctccttcggc ttctccgcca ttggactcct ccatctgtca gactcgtggc agggcgtgcc
     3841 tgtgccctgc acgccctgag tctggacgca gtcggcaaac ggcatgcctc acagatttgt
     3901 caccttcata tcatgctaca acaactaata ccataatcta cattgctatg atacaatcta
     3961 caatgtactt gtggctttga tgcgccttgc ctccagtgtc gcggcatagt cgcaggcatg
     4021 aaacctgtta ttgatgacgc ctgggagagg gggtgagaag cgctcgtacg gactcgagcc
     4081 cttatgcggg tagtggtgct gtagagtggc tggagggatc aacaggatgg caccatttgt
     4141 gctcatgtag ttccctccta cgttctatat caacatagag gacggaagca acgacatagc
     4201 tctcgaggga ggagcgcgga acagatgaca gcacttggac agaaggtcaa ggagttgagg
     4261 gtctctaaag gaatgactca gcagcggttg gccgaaaagg cagaggtcag tcactcgctg
     4321 attagtgcac tcgagtctgg acgccgaaag tacgtctcag ctcaggatat tgagcgtctc
     4381 gcgaaggctc tggacgtgcc ggccaacgac ttctggaaac tggtacccgg cggcaagagc
     4441 gagcagcgat acatacccat caccggaacc ggccggagcg agatggccaa atcggcctga
     4501 aaccccagca ggaggactgg ccgcccacag gagggtagcc ttgggcggcc agtcgcagga
     4561 cttaccgccg cccgaactcc ctttccagtt gactgcgact gagcagtatt gccgtcggtc
     4621 gaccgtggga acagtgttga ttgatgccgg cctcctccag tgcctccacc agcgctcgca
     4681 tctcctccgc actcaggggc tggccggcct tgacggcgct ccggcaggag agggcgacgg
     4741 ccattcgttc ccgccagtcc gcctctccgg cgttgtcggt caactcctga agcgattgct
     4801 cgagcgcctg tactgcccga gaggacggta gttcgctcgg tattcccctc acgaggatgg
     4861 actgctcccc aaaaggctcg aggcggaagc ctagcgactc cagagccgag ccagccgcct
     4921 cggcgatctc cctctgaagt gccgtcacct ccagcactat cggctccagg agaagttgcg
     4981 tgtttccctc gcggtccatc gactgatgca gcctctccag cagcacccgc tcgtgggccg
     5041 cgtgctggtc gatcaggtac aacccctgtt caccttcggc aactatgtag ctctgagcga
     5101 cctgtcccat cagccggagg atgggaagcc ggtgaccacc cactggggct gccggtgccg
     5161 ccgcattggc cgtaatcgcc gtgtcgatca gtctcggcat agagacggga gactggccgg
     5221 gtgtgggcgg cgaggtcgga gggattgtct cccagggcgc gatcttctcc tgaggtgcct
     5281 ccagtcccga gacctgctgt gcccagctcg aaccgcccgc cagggtctcc ctaaccgcgt
     5341 ccctcaacgt cgcatagatt gccctctctc ttagcagccg cacctccgtc ttggtggggt
     5401 ggacgttgac gtcgacctcc gtgggtggta cctgaagatt cacgaaggcg atagggtggc
     5461 ggcccacctg cagcagcgtc tgatacgact cctccaccgc gaacgagagg ctgtgactct
     5521 tgatgaagcg acggttgacg aagagccaga tggaggaacg ggaggctcta ctgactcccg
     5581 ggtcggcgac ataccccgac accctgatgg cagttctgcc ctgggcgtga tcaacctcgg
     5641 tggaggcttc cacgggaagc aggaacccgg caacgtcctt gccaagaacc tgcatggccg
     5701 catccagcgg attgccgctt cccggtgtcg tcagcgactt ccgaccctct atctggagtt
     5761 gaaacgcaac ctctggataa gcgatcgcat actggctgac gagttgagcg acctgagcag
     5821 cctccgcggc gtttgtgcgg aggaacttgc gcctggcggg tacgttgtag aacaaccgct
     5881 ccacagaaat ggttgtccca acagctctcg cggagaccgt ggcagggccg gtgaccccgc
     5941 cgtttgacct gagctgaaat gccgatgctt cacctgccgt gcgactaagc accgtcaact
     6001 cggagactgc ggcgatgctt gcgagcgcct ctccccgaaa ccccagggtg gagatagaag
     6061 atagctcctc ggccgagctg atcttgctgg ttgcgtgccg ctccaacgcc agcggtagct
     6121 cgtcgcgggg gatgccgcag ccgtcatcgg ctatcctgat caggtcggtg ccaccgccgg
     6181 caatttccag atggatcgtc cgggcaccag cgtcgagtga gttctccagc aactccttga
     6241 cgactgaggc aggccgctct actacctccc ccgcggcaat cttgccgaca gtatcagcgc
     6301 tcaggaggtg gattcccatt agatctctgt cgcctttctt tggagctcat ccagcttcgc
     6361 gagcgcttgg agcggtgtca tgtttgagag atccatcgtc gcgattgtct cgaccaactc
     6421 ggcgagttcg tgcccctgtc catcctgctg gtgcggttcg gctcgacccg gggtagcggc
     6481 ggccgattcc agttcttcca gtatctgcct cgctctgcgt atcacgggga tagggactcc
     6541 tgccagttgc gctacgtgaa tcccatagct acggtcggca ccaccgggca cgacgcggtg
     6601 caggaacacc acgcggcccc cttcctccag gacgtctacc cgctggttct tcactcgggg
     6661 gaggagcgat tctagctccg tcaactcgtg gtagtgcgtc gcaaacagcg tcttgcaccg
     6721 cagcttcggg tcgttgtgca aatgctcgac gacggcccgg gcaatggcca ttccgtcgta
     6781 ggtgctggtg ccgcgtccga cctcatcgag gagcaccagg cttcggggcg tggcgttgcg
     6841 cagcaggttg gccgtctccg ccatctccac aaggaaggtg ctcttccctc cggtcacatc
     6901 gtgctgagcg ccaaccctgg tgaacacccg atccaccagg ccgatgtgtg cctcctctgc
     6961 agggacgaag ctgcccatct gtgccatcaa cacgatcaaa gcgacgccga gaacgtatgt
     7021 ggacttgcct gccatgttcg ggccggtcag cacgacgatc tgggaggaat cgctgtccag
     7081 gaagcagtca ttggagacgt actctccgat cgagatcgtg cgctcgacga tggggtgacg
     7141 tccctcgcgg atggagatca cgctgccgtc atcgaccgtc ggacgtacgt accggttggc
     7201 gactgccacc tctgccaggc tcagcaacgc atccaccctg gcgatggact gtgcggttct
     7261 cctgatcctg ccggccaagc ctgccagctc ggtaaccagc tcggcgtaga tccgcttctc
     7321 cagcgcttcg atctcttctt gtgcgctggc gaggcgcacc tcctgctcct tgagctgtac
     7381 cgtgacgaag cgttccgcgt ttgccagtgt ttgcttccgc tggtagccca tcgtctccag
     7441 catctctccc acggtgccgg cgcctgtctt caccctcgca aactcgtcgg taggtgcatc
     7501 aaggtttggc cttgtcacct cgatgtagta gccgaatacc ttgttgtagc cgatcttcag
     7561 cgacttgacg cctgttcgct cacgctcgcc atgttcgaga ccggctatcc atttgcgtgt
     7621 gtccccgctg atcccgcgca gatcatcgag atcctgcgac ttaccctcgc gaatcacacc
     7681 ctgtccgaca acgacggccg gctcgtctgc aatggtcgac gatattgcgt cgatcgcctc
     7741 gggacaggcg tcgagagcgg cgctctcagc agcgaggata cccggcagcc actgcttcag
     7801 cccctgcagt agcctgggga cgacccggag cgatctggca atcgcgatgc agtcgcgggg
     7861 agagacgcct tgttgtgctg ctctggatgc caacctttcc aggtcgccga gacctgccag
     7921 gttcttggag agatcggctc gtcccaatgg ggaggagacc aactcctgca ccgcattcag
     7981 ccttgcttcc accgaatcga gatcgaggag cggctgcccg acccagcgac gcatcagtct
     8041 gccgcccatg ggggtcagcg tgcggtccag tacacccaac agggacccct cgacgttccc
     8101 agtcactgca ttggcggtga tctccaagtg gtgccgggct gccgaatcga gcttcatgta
     8161 gcgatccagg ctgtagacct gcagcgactc caattgcccc agagatgcgg gctgcgtgtc
     8221 ttgcaggtag acgaggagtc tgacagctgc tctggtagcg gctgaccctg gggtgaggca
     8281 aaagc
//
LOCUS       DATALS010000080         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_408971, whole genome shotgun sequence.
ACCESSION   DATALS010000080 DATALS010000000
VERSION     DATALS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408971"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VHS06_01670"
     CDS             <1..298
                     /locus_tag="VHS06_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX2986866.1"
                     /translation="VDVSTGIALPEPEDLDAFRLTPDHVLDLTEAIRQYVIVESPLQP
                     LCDEECKGLCSQCGANLNLGPCDCQQLASSVPQGNLGVLLAERLRQAGFKPEQE"
     gene            305..502
                     /gene="rpmF"
                     /locus_tag="VHS06_01675"
     CDS             305..502
                     /gene="rpmF"
                     /locus_tag="VHS06_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX2986867.1"
                     /translation="MGALPKKKISHSAQGKRRSHLGIEMPDLMTCPQCRALKLPHHVC
                     PNCGTYKGEQVLPIKPRKSEG"
     gene            444..1481
                     /gene="fabD"
                     /locus_tag="VHS06_01680"
     CDS             444..1481
                     /gene="fabD"
                     /locus_tag="VHS06_01680"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX2986868.1"
                     /translation="MARTRASRFSRSSRGRARARKLVPKIAFVFPGQGAQKVGMGASL
                     AQCSSAAREVFGKADSATALPLSHVCFDGPQERLTDTAFAQPGVVAVSLAAAAALSEG
                     LAARGISTIPALCAGHSVGELAALSFSGAVSLDEGLRLVAARGRLMAEASAMVDGTMA
                     AVLGLEEGPLVDVCSAASKETGESVQVANLNAPGQIVISGHRKAIQRAGQLAKDAGAR
                     RIVELVVSGPFHSVYMRPAADGFRGPMAETSVVSPQVPLVLNTSAAISSDPAEIRREL
                     EVQVTSPVRWTDSVRTMLDAGCTVFVELGPGQVLGGLIKRVTKDALILNVEDEVSLNG
                     TMNKLAEVLSD"
     gene            complement(1497..1760)
                     /locus_tag="VHS06_01685"
                     /pseudo
     CDS             complement(1497..1760)
                     /locus_tag="VHS06_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
     gene            complement(1760..>2377)
                     /gene="fabG"
                     /locus_tag="VHS06_01690"
     CDS             complement(1760..>2377)
                     /gene="fabG"
                     /locus_tag="VHS06_01690"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX2986869.1"
                     /translation="REVVEAISAAGGRASTVQADVSKPAEAEAMVDSVVKEFGALDIL
                     VNNAGITRDGLVMRMSEADWDAVLDTNLKGAFICTKAALRSMMRRRSGRIVNVSSVAG
                     LMGNAGQANYSASKAGVIGFTRAVAREVASRNITVNAVAPGYIETDIWSSVSEQARSG
                     LMQLIPMGRAGQPEEVAEVVAFLASDAARYMTGQVLSVDGGMVMG"
BASE COUNT          448 a          731 c          733 g          465 t
ORIGIN      
        1 ggtggacgtg agtacaggga ttgctctccc cgaaccggaa gacctcgacg ccttcaggct
       61 cacaccggac cacgtgctgg atctaaccga ggcgatccgg cagtacgtga tcgtggaatc
      121 tcccctgcag cctctctgtg acgaggagtg taaggggttg tgtagccagt gtggtgctaa
      181 cctgaacctc ggaccatgtg attgccagca gttggcttcc agcgtcccac aggggaacct
      241 gggagtgttg ttggcagagc gattgcgtca ggccggtttt aagccagaac aggagtagac
      301 agaattgggt gctctaccta agaagaagat ttcccattcc gcacagggca agcgccggag
      361 ccatctgggc atcgagatgc ccgacctcat gacttgtcct cagtgccgtg ctctgaagct
      421 gccccaccac gtgtgcccca actgtggcac gtacaagggc gagcaggttc tcccgatcaa
      481 gccgaggaag agcgagggct aggaaactgg ttccgaagat agctttcgtt tttcccggcc
      541 aaggcgctca gaaggtcggt atgggggcca gtctggccca gtgcagttct gcggctcgag
      601 aggttttcgg caaggccgac agcgcaaccg cgttgcccct gagtcatgtg tgtttcgacg
      661 ggccgcagga gaggcttacg gacaccgcat ttgcccagcc gggagtggtg gcggttagcc
      721 tggccgcggc tgctgcgttg tccgagggac ttgccgcaag gggcatctcc acgattccgg
      781 cgctgtgcgc cggccacagc gtgggtgagc ttgcggctct gagcttcagc ggcgcggtga
      841 gtctcgatga ggggcttcgg ctggttgccg ctcgaggacg gctgatggcc gaggcatccg
      901 cgatggtcga cggcaccatg gcggcggtgc tgggtctcga ggagggaccg ctggtagacg
      961 tctgctctgc tgcctccaaa gagacagggg agtctgttca ggtcgcgaac ctcaatgctc
     1021 ccggtcagat agtcatatct gggcatcgca aggcgatcca gcgggccggt caactggcca
     1081 aggatgccgg cgctcggcgg atcgtggagt tggtggtcag cggtcctttt cactccgtct
     1141 atatgcggcc cgcagccgac ggcttccgtg ggccgatggc ggagacgtcg gtggtttccc
     1201 ctcaagttcc tttagttctc aacaccagtg cggccatatc gtctgatccg gcggagattc
     1261 ggcgcgagtt ggaggttcag gtcaccagcc ccgttcggtg gacagattcg gtgaggacca
     1321 tgctggacgc cggatgtacc gttttcgtcg aactgggacc cggccaggta ctgggtggat
     1381 tgatcaaaag ggtcaccaag gacgccctta tcctcaacgt cgaggacgaa gtcagcctca
     1441 acggtacgat gaacaagctc gccgaggtgt tgagcgacta gtcctgcagc ataattgttc
     1501 tcggatagga agccgtagcc ggggtgtata gcctcggcgc cgctgaccat cgcggcgctc
     1561 acgacgtttg ggatgttcag gtagctctta tcggaagccg ccggaccaat gcagatcgcc
     1621 tcgtccgcca actcgaccgc cagggagtga cgatctgcct cggagtaggc gatcacggtc
     1681 gcgactccca gctcccggca ggctctgatg atccgaacgg ctatctcgcc ccgattggcg
     1741 accaggatct tatggaacac taccccatca ccatcccgcc gtcgactgag agcacctgcc
     1801 ctgtcatata gcgagcggca tcagaggcaa ggaaggccac cacctccgca acctcttccg
     1861 gctgcccggc ccggcccata gggatcaact gcatcaagcc gctcctggcc tgctccgaca
     1921 cactgctcca gatgtcggtc tcgatgtaac caggagccac ggcattgacg gttatgtttc
     1981 ggctcgctac ctctcgcgca accgccctcg tgaacccaat cacgcccgcc ttggaagcag
     2041 agtagttggc ctggccggca ttgcccatca acccagctac agaactcacg ttcacgatgc
     2101 gcccactcct gcgccgcatc atcgagcgaa gagccgcctt ggtgcagatg aaggcgccct
     2161 tgaggttggt gtccagcaca gcgtcccagt ccgcctccga catcctcatg accaggccgt
     2221 ccctggtaat cccagcgttg ttcaccagga tatcgagagc accgaactcc tttacaacac
     2281 tgtccaccat cgcctcagct tctgcaggct tggagacgtc ggcctgcacc gtagaggctc
     2341 ggcctccagc ggccgaaatc gcctccacca cctcgcg
//
LOCUS       DATALS010000081         4277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_232147, whole genome shotgun sequence.
ACCESSION   DATALS010000081 DATALS010000000
VERSION     DATALS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232147"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="VHS06_01695"
     CDS             <1..1359
                     /locus_tag="VHS06_01695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2986870.1"
                     /translation="GTVTGARFLVSTERARVLVDCGLFQGLKRLRLLNWEQPSVDPRS
                     IDAIVLTHAHLDHTGYLPRLVNSGYDGPIYGTPATLELCGILLPDSGHLQEEQAEHAN
                     LHGYSKHSPALPLYTSLDATNTLQYLRPCPYGEPREIAEGIRVRLVDAGHILGSAIIE
                     MWVQDRGGEIKITFSGDLGRYGEPLLGDPTPIEDTDYLLVESTYGDRTHSNGHPERQL
                     EEMVLRAAEMKGCLLIPAFSVGRTQQLLYAFHCMEKEGRFPDMPVYVDSPMAIDATKI
                     FLSHPEAHRVDLRHLESDGENPLRLNRLKFVRTPEESRTLDERQEPMVLISASGMATG
                     GRILHHLASYLDDPRATVLFVGYQAEGTRGRTLLDGGKHLKMMGKEVPVRATIRQIDG
                     FSGHADRDEIVRWLSGFKRAPKTAFVIHGEDHAAGAMAGTLQSRFGWKTAHPALRETV
                     EL"
     gene            1374..1937
                     /locus_tag="VHS06_01700"
     CDS             1374..1937
                     /locus_tag="VHS06_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2986871.1"
                     /translation="MPRIWVGEDEGRRALLQDGVILSVAVEGAEPPSGYWAAMLPHGS
                     PSSALILGLGGGTLAHLLSRRYQGIQIVGIDTDVELIDFARREFDLDLPNLEVVIQDA
                     FEYVAQCRRHFDYVAVDLFAGYDFQRGVVAKPFLRHLKEIVGPGGEITFNIFRDKRTE
                     THLNRISRVLRIKQKLVVGRNVVVHCG"
     gene            2131..2829
                     /locus_tag="VHS06_01705"
     CDS             2131..2829
                     /locus_tag="VHS06_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX2986872.1"
                     /translation="MPAGDFSSWLAEIVEAIRGERTSDVPCDGCTACCTSSQFVHIDP
                     SDADTLSHVSPKLRFPAPFMPRGHFVLGYDERGHCPMMVDSHCTIYEHRPRTCRTYDC
                     RVFRAAGIEADEDKVLIARQARRWQFSFPTEADRILHAAVKAAAAFIREHPDELPAGT
                     APNNATRLAVLAIQLHHLFLQRDETPTRATVVTPSAEVIRTEMMRLAGARSTEKTDGG
                     EARISRRPPLHRRR"
     gene            complement(2861..3937)
                     /gene="rsgA"
                     /locus_tag="VHS06_01710"
     CDS             complement(2861..3937)
                     /gene="rsgA"
                     /locus_tag="VHS06_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX2986873.1"
                     /translation="MYELSALGFGPFFERQLQPCDCHEVILARIAAEHRGGYEVWSPV
                     GAGPAQLAGRLRLEVDETGLPGVGDWVTLKTAPGPDRTTVIDRVLERRTVFTRGAAGS
                     QSRAQVIVSNVDLVFAVCGLDGDFNVHRIERYLARIWASGAQPAVILNKADICEDLAA
                     RVAEVEEQCPGVSVYVTSALRSEGVAAVRECIQTGLTAALVGSSGAGKSTLVNALLGE
                     VRMRTGDVRAIDSRGRHTTTSRQLVLLPDGGLLLDTPGMRELQLVDEDGLDAVFGDIA
                     TLSERCRFHDCRHEAEPGCAVREAVESGVLAAERLEHYRRLERESQAYERRHDERLRR
                     QSERVWGQLYAEVKQLRRWKGGKE"
BASE COUNT          819 a         1409 c         1322 g          727 t
ORIGIN      
        1 ggtaccgtca ccggcgccag attcctggta tccacggaga gggcaagagt cctggtggac
       61 tgcggcctct tccaaggtct caagcggctc cgactgctga actgggagca gccgagcgtg
      121 gatcctcgct ccatcgacgc gatagtgcta acccatgctc acctggacca cacgggttac
      181 ctgccgcggc tggtgaattc cggctacgac ggcccgatct acggcacacc cgcgacgtta
      241 gagctgtgcg ggatccttct gccggactcc ggccacctgc aggaagagca ggccgagcac
      301 gccaacctcc acggatactc caagcactcg cccgcgttgc cgctttacac ttccctggat
      361 gcgacgaaca ccctgcaata ccttcgtccg tgcccctacg gcgagcctag agagatcgcc
      421 gaggggatcc gtgtccggtt ggtggatgcc ggccacatcc tcggttccgc aatcatcgag
      481 atgtgggtcc aagatagggg tggggagatc aagatcacct tctccggcga cctgggccga
      541 tacggcgagc cgctgttggg cgatccgact ccaatagaag acacggatta cctcctggtg
      601 gagtccactt acggcgaccg cacccactcc aacggacacc cggagagaca actggaggag
      661 atggtgctga gggctgcgga gatgaaggga tgcctcttga tccctgcctt ctccgtcggt
      721 cgtactcagc agctgttgta tgccttccac tgcatggaga aggaaggccg cttccctgac
      781 atgccggtgt acgtggacag ccctatggcc atcgacgcca ccaagatctt cctgtcccac
      841 ccggaggccc acagggtgga cctgcggcac ctggagagcg acggcgaaaa cccgctgagg
      901 ctcaaccggc tcaagttcgt gcgcacccct gaagagtccc gaaccctcga cgagcgacag
      961 gagcccatgg tgctcatctc cgccagcggc atggcgaccg gcggccgcat cctgcaccac
     1021 ctcgcctcct acctggacga cccgagagcg acggtgctgt tcgtcggcta ccaggctgag
     1081 ggtacccgcg gccgcaccct gctggatggc ggcaagcatc tgaagatgat gggcaaagag
     1141 gtacccgtga gggccaccat caggcagata gatggcttct ccggccacgc cgatcgcgac
     1201 gagattgtcc gatggctctc cggcttcaag agggccccga agacggcctt cgtgatccac
     1261 ggcgaagacc atgcagccgg tgccatggcc ggcaccttgc agagccgatt cgggtggaag
     1321 accgcgcatc ccgcgctccg cgagactgtc gagctatagc agggggacat cgattgccgc
     1381 gcatctgggt cggcgaagac gaagggcgaa gggcgctgct gcaggacggc gtgatcctct
     1441 cggtggcagt ggagggagcg gagcctcctt ccggctactg ggcggcgatg ctgccccatg
     1501 gatcgccttc gtcggcgttg atcctggggt tgggcggcgg cacgctggcg cacctcctct
     1561 ccagacgtta tcaggggatc cagatcgtag ggatagacac cgacgtggag ttgatcgact
     1621 ttgctcgtcg agagttcgac ctggatctcc ccaacctgga ggtggtgata caggacgcat
     1681 tcgagtacgt cgcgcaatgc cggcggcatt tcgactacgt ggccgtcgac ctcttcgccg
     1741 gctacgactt ccagcgcggc gtggtggcca agccgttcct ccgacacctg aaggagatcg
     1801 tcgggccggg cggagagatt accttcaaca ttttcaggga caagcggacg gaaacccacc
     1861 tcaaccgaat cagcagggtg ctgaggatca agcaaaagct ggtagtgggc cgcaacgtcg
     1921 tagtccactg tgggtaggca tgtgagacgc cgggtctgaa cgtagggcag gaccctgtgc
     1981 cctgccggca ggtggtcgta gccaaacgat tgcggcgggg gacaggcccc cgccctacat
     2041 gccaacatca ggcgtgtcac agaactcgac gggaacagag aggaacaccg aacatccatg
     2101 cccgacgata gcacgaagga acaacgacac ttgccagccg gggacttctc atcgtggctg
     2161 gccgagatag tcgaggcaat acgcggcgag cgcacgtcgg acgtgccgtg cgatggctgc
     2221 actgcttgct gcacctcgtc ccagttcgtc cacatcgacc cgagcgatgc cgacacgctc
     2281 tcccacgttt ccccgaagct gcggttcccc gcccccttca tgccccgcgg ccacttcgtc
     2341 ctcgggtacg acgagcgagg gcactgtccg atgatggtcg acagccactg cacgatctac
     2401 gaacaccgcc ccaggacctg ccgtacctac gactgcaggg tctttcgcgc tgcagggatc
     2461 gaagccgatg aggacaaagt cctgatcgct cggcaagctc gccgctggca gttcagcttc
     2521 cccacggagg ccgaccgaat ccttcacgct gcagtcaagg cggccgccgc cttcattcgc
     2581 gagcatccgg acgaactgcc ggccggcacg gctccgaaca acgcgactcg gctcgccgtg
     2641 ctcgcgatcc agcttcacca tctcttcctt cagcgcgatg agacacccac ccgcgccaca
     2701 gtcgttactc ccagtgccga agtgattcga accgagatga tgcgcctggc aggagcccgg
     2761 tcaaccgaaa aaaccgacgg cggtgaagcg cggatctcga ggcgaccgcc gcttcaccgc
     2821 cgtcgctgac catctgccaa cgcggaggcc tactgcttcg tcactccttg ccccccttcc
     2881 agcgccgcag ctgcttcact tccgcgtaga gctggcccca tactcgctcg gattgtcgcc
     2941 gcaaccgctc atcatggcgc cgctcgtagg cctggctctc gcgttcgagt ctccggtagt
     3001 gttccaggcg ttcggcggcg agaacgcccg actccaccgc ctcccgcact gcacaacccg
     3061 gttcggcctc gtgccggcaa tcgtggaagc ggcaccgctc ggagagtgtc gcgatgtccc
     3121 cgaacaccgc gtcgaggcca tcttcgtcca caagctgcag ctctcgcatg cctggtgtat
     3181 cgagcaggag gccgccatcc ggcagaagga cgagctggcg gctggtcgtg gtgtggcggc
     3241 cgcggctatc gattgctcgc acatcaccgg tgcgcatccg cacttccccc agaagcgcgt
     3301 tgaccagcgt cgacttgccc gcccccgagg agccaacgag ggccgccgtc aggccggtct
     3361 gaatgcactc gcgtacagct gctacgcctt ccgagcgcaa tgcgctcgtt acgtaaaccg
     3421 acacgccggg gcactgctcc tcgacctcgg ccacccgcgc agccaggtcc tcgcagatat
     3481 cggccttgtt gaggatcacg gcgggttgtg cgccgctagc ccaaattcgg gcgaggtagc
     3541 gctcgatcct gtgaacgttg aagtcaccgt cgaggccgca aacggcgaac accaggtcga
     3601 cgttggagac tatcacctgg gcgcgtgact gggagcccgc ggctccccgt gtgaaaaccg
     3661 tacgtctctc aagcacgcgg tcgatgacgg tggttcgatc gggtccaggt gcagtcttca
     3721 gggtgaccca gtcgcctacg cctggcaaac cggtctcgtc cacctccagc cgaagccgtc
     3781 cggcgagttg tgcagggccc gcgccgactg gggaccagac ctcgtagccg ccacggtgtt
     3841 cggcggcgat gcgggcaagg attacttcgt ggcaatcgca aggctgaagc tgtcgttcga
     3901 agaatggacc aaagcccaga gcggatagct cgtacaacct aacgtcctcc atgtgcgcgg
     3961 cgcagagcac gcgcgattac ccgcgtacgc tgagcaccga cgtgatcggc gaggcacgcg
     4021 ggagggatca actgttgagg agatgagaca cgccgaggcg ctcagcgcct acgacgcact
     4081 cgaaccctca cgcaagatcc ttatggagaa cggtggcggt catcaagcct ccgaaaaggg
     4141 gaataagggc gccgtggaga ttgtagctcc attggcgccc gactgcaaag ctcagtgggc
     4201 tccggccaga atgacctgat cgccctgatt cagtacgagg gatctagtag ccggactgcc
     4261 gttaaccaac gccatct
//
LOCUS       DATALS010000082        15631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_273655, whole genome shotgun sequence.
ACCESSION   DATALS010000082 DATALS010000000
VERSION     DATALS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 15631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15631
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273655"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VHS06_01715"
     CDS             complement(<1..1364)
                     /locus_tag="VHS06_01715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2986874.1"
                     /translation="MPRDSIIPGPAYSAKWLLPFGVVVLSLTLRVWNITSLPIFIDES
                     NNLRWAYDMLEQRTRMSLLIPIHEDGKQPLFIWLVALAMRFFADPLVAGRIVSALAGS
                     AATLGILLLGRWLDTWRTGLVAAFLYALAPYPVFFDRIGLMDGTIAAASVWASCLGVR
                     IAMGPSTQVRAILLGAALGTMMGIAVWVKMTGLFVLPIPLLCLLLVRKRRDLARACLA
                     LAVGYGLFALLAGIMVLLPEAENLIEKGSHFSLTPAEILTFPLALWGKNAAAYISWLQ
                     TYLPSPLSWLVLAAVVWGLGTRRRETLLLLGCWISLAVPPILVGKDLFTSRYLVPSIP
                     PLVVMVGLLMVKTWERGARAVHERLSLPHVHSISRGIGLLLLLATVAPSAAFDIQLLT
                     DPSRADLVEFDRFQYISGFPSGYGFREAIELIKSRAAEQGGEVIVLTDDFRMLPRDGA
                     LVYL"
     gene            1960..2238
                     /locus_tag="VHS06_01720"
     CDS             1960..2238
                     /locus_tag="VHS06_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX2986875.1"
                     /translation="MREHADYFTQTSPEPMTEEDEETLDWLNRALAGDPRTTDAIIMV
                     EVHGGEVVLRGDTQVPGVKPAAEDVAWGVPGVHSVVNQIVAHGAASGE"
     gene            2314..2964
                     /gene="tenA"
                     /locus_tag="VHS06_01725"
     CDS             2314..2964
                     /gene="tenA"
                     /locus_tag="VHS06_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEX2986876.1"
                     /translation="MEFSDTLLGRAASVREDVFAHPFVRGLGDGTLPEPKFRFYMCQD
                     YLFLIEYCRVFGLATAKGEDLATMARFAELLHSTLGVEMELHRRYAAEFGLSRNDLEE
                     TEIAPATHAYTSHLLNVAWSSSVGCIAASLLPCQWGYWEIGCRLAQLPGLEQNRYRRW
                     VESYSLYEFGELARWLRGLVDEVAATASPAELGRMEREFMASSRYELMFWDMAWKA"
     gene            3117..3581
                     /locus_tag="VHS06_01730"
     CDS             3117..3581
                     /locus_tag="VHS06_01730"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX2986877.1"
                     /translation="MIGVLEAGLRAGHHSLWRLATDWLPAVVVMGLIFWLSSQSVLPG
                     PADKGMDFLFKKSAHLMAYSVLALSYLRAVHAARRPYLVAFVLTVLWAISDEIHQSTV
                     PLREPTFRDVLIDATGGGVALWLVLRSIAHTQSAVGEVLRAAMRIGAGTGAG"
     gene            complement(3617..4126)
                     /locus_tag="VHS06_01735"
     CDS             complement(3617..4126)
                     /locus_tag="VHS06_01735"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEX2986878.1"
                     /translation="MSQTRHEFGPVMRLEADALGEPGQRTFCLLAAAREGSAALWLEK
                     EELQALGVAIDQLLARLSDRPEWKLYGAPAAEYQPSQGFSPEPLVEFKVGQLSLGYES
                     DSDLFALLVHDAEDDPEGPATFSCLASAAQMRALSRRIETVISAGRPRCPLCGDAIEP
                     EGHNCVRAN"
     gene            4264..6114
                     /gene="iorA"
                     /locus_tag="VHS06_01740"
     CDS             4264..6114
                     /gene="iorA"
                     /locus_tag="VHS06_01740"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX2986879.1"
                     /translation="MKQLLSGNEAIALGAYHGGVSVAAAYPGTPSTEILETIARYTEL
                     HAEWAPNEKVAMEVALGASYAGVRTICSMKHVGLNVAADPFFGAAITGVVGGLVVVTA
                     DDPGMHSSQNEQDNRHYARFAKVPVLEPSDSQEAYEMTRLAFVLSEQFDTPVLLRTTT
                     RTSHSKSVVEYDETTMPERRGASFVRQPEKFVMIPAHARRRHPLVEERIAKLEEYAEG
                     FAYNRVEMADASLGIISAGVAYQYARDVMPNASFLKLGMSYPLPARMILEFASRVRRL
                     MVVEELDPFIEETVRGLGVQVEGKQFFPAVGELSVERVEEGFSAAGAVPAGEEMVPAS
                     SEPGADKEEPLPLRPPVLCPGCPHTGTFFTLKRLGFYRGTVDAGATSQGQLLGRVKRT
                     GLVVSGDIGCYTLAVLPPLLALDATTCMGASIGMAMGMERAGLANKVVAVIGDSTFLH
                     SGITPLLDVVYNGGTITTIILDNSTTAMTGHQENPSTGFSAKGKPAPAADLEKIVRGL
                     GVEDVKVVNAFDLDAISSAVKDSVDRDEPSVVIVRGDCAVKVRGVGALSVVDAEACNG
                     CSTCLRLGCPAIARVEDKARIDPTLCVGSRCGLCRRVCPINAIKEQVSNG"
     gene            6107..6694
                     /locus_tag="VHS06_01745"
     CDS             6107..6694
                     /locus_tag="VHS06_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HEX2986880.1"
                     /translation="MDKLDFVLAGVGGQGIVLMSDLLGDAALAAGYDVKKSDVHGMAQ
                     RGGSVVSYVRLGAKVSSPLPKMGEVEFLVALEKLEAARWAPYLRPGGVAVVNDHAIFP
                     LSVSAGASSYPSDEQVVATLRERTERIYQIKGPEIAVGLANPKVLNVVILGFLAALLP
                     IPWDIWTATVEHRVPPKYRELNLRALEAGRAAARQ"
     gene            7196..8500
                     /locus_tag="VHS06_01750"
     CDS             7196..8500
                     /locus_tag="VHS06_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX2986881.1"
                     /translation="MVAYWDPERETMARADMEALQLELLQKKVAEVYERVPFYRAAFQ
                     ERKVTPGDVRSLADLAKLPFTDKTAFRDNYPYGLLARPRKEVVRIHGSSGTTGKPIVV
                     AYTRNDVDLWANMVARSLAMGGVTDEDVVHIAYGYGLFTGGLGIHYGAERLGATVLPM
                     SSGNTKRQIMLIQDMGSTVIACTPSYSLFLAETALEMGCDLRDSSLRVGFFGAEPWSY
                     RMREEIEAKLGILALDIYGLSEIVGPGAAQECPHKQGIHIFEDHFLPEVIDPVTEEPL
                     PDGEKGELVITTLTKEALPVIRYRTRDITSLHREKCACGRTLVRMDKVIGRTDDMLIV
                     RGINVFPSQIESVLLAVEGVEPHYRIVVDRQKSLDDLEIQVEVSEAVISDEVGRLERI
                     GRKVRAELESVLGISALVKLVEPGTIVRSEGKAKRVVDRREL"
     gene            8552..8938
                     /locus_tag="VHS06_01755"
     CDS             8552..8938
                     /locus_tag="VHS06_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HEX2986882.1"
                     /translation="MKQVSVFVENRPGRLKLLLAALDRMEVNIRALSIADTADFGIVR
                     MILSDTEKGIEAVKGAGFAASTNEVLAVDIPDQPGGLLHTVAEPLSTAGVNIEYVYAF
                     VDSPLDKARAVLKVGDIDAAEKLIGK"
     gene            8935..10815
                     /locus_tag="VHS06_01760"
     CDS             8935..10815
                     /locus_tag="VHS06_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX2986883.1"
                     /translation="MSGSSFQGRLLVVDLGSRSAQTLPLDSSYCRLFLGGVGLATRLL
                     WDYSHPGVDPLSPENPMVFATGPLTGTMMPAASKYAIATKSPLTGFIGDSLSSSGWPL
                     ALKRAGYDAMVVLGAAEEPTYLFIDDERVYFRRAVSVWGGECWETEQRIRQDLGDESV
                     QVASIGVAGERLVRYACVGNGFGRQAGRTGPGAVMGSKKLKAIAVRGTKTVEVTDPAG
                     LRDGCVDLYHRSQGAATEKYRTLGTPGNVLVLNKLAALPTRNFRQSTFEGAERVSGEW
                     LQQRHLGKVVACAGCSIGCDHIYRISEGPSAGDQVAMDYESLYALGPLCGVDDANAIL
                     RASELCDRYGTDTMSTGVSIAWAMECFERGLISREMAGGLDLRFGNGDALVTMVEQIG
                     KRRGLGRLLGEGVKRAAAEVGGGSERFAMHVKGLEMPGYEPRSLKTLALGLAVGTRGG
                     CHNRSAAYEVDLSAKVDRLNGERSRGPLAMAQEDFAATLDSLGICKFLRKCFDAFYAE
                     TSALFRAGTGLDMTTEELQQVGERVSNLKKAYNVREGWKPEDDWLPDRLFDEPLPTGV
                     AKGTVLTREELREMIQGYYDARGWTAEGQIPASKLAQLGLGDLAAGDEKGATDAATAD
                     TL"
     gene            10790..11263
                     /locus_tag="VHS06_01765"
     CDS             10790..11263
                     /locus_tag="VHS06_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2986884.1"
                     /translation="MQRRLILCDPTLCTGCRLCEFACALEKTGSFDLELSRIRVASPQ
                     PDSVISISCQLCESAPCVEACPRDALTIRSDDGTIALNKALCSGCGWCIEACDFGAIA
                     LDRSTKSVAICDLCRETAQPRCVESCPKKALSLAPKSTAAGRSRKKAAEQLLRRA"
     gene            complement(11387..12073)
                     /locus_tag="VHS06_01770"
     CDS             complement(11387..12073)
                     /locus_tag="VHS06_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEX2986885.1"
                     /translation="MHGEILAHEVVPDGSNYTFFVLISADGHGRFLAVYKPQRGERPL
                     WDFPRGTLHQREHAAYVVSRHLGWPNIPVTVIRDGPFGIGSVQLYVPIQDGADYFSFR
                     HKRRRDLMQIALFDLLVNNADRKGGHCLLGEDGRLWAIDHGLTFHVEPKLRTILWDYC
                     GQPVPQRLLEQMQALREDEGRSQKLREALEPDLEPAEIKAFFRRIDRILKVRHYPQLD
                     PNRNVPWPPI"
     gene            complement(12555..12752)
                     /locus_tag="VHS06_01775"
     CDS             complement(12555..12752)
                     /locus_tag="VHS06_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX2986886.1"
                     /translation="MKRLYRSRRERIIAGVCGGMAEYFDVDPTIIRVIWVLGAMVTMF
                     FPGVLLYFVCWAIIPPAPATV"
     gene            12935..13198
                     /gene="rpsP"
                     /locus_tag="VHS06_01780"
     CDS             12935..13198
                     /gene="rpsP"
                     /locus_tag="VHS06_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX2986887.1"
                     /translation="MIKIRLRRTGGHKQPSYRIVVADVRAPRDGQYIDLIGNYNPRTE
                     PATIVVDAEKANKWFQQGAQMTETVEKLFARAGVLEQVKARKA"
     gene            13231..13458
                     /locus_tag="VHS06_01785"
     CDS             13231..13458
                     /locus_tag="VHS06_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX2986888.1"
                     /translation="MKELVEFIARSLVDTPDAVKVTEIETDQMTTYEIEVDQPDIGKL
                     IGRQGRIVKAIRSVVRAAAARQGKRVAVEVV"
     gene            13486..13986
                     /gene="rimM"
                     /locus_tag="VHS06_01790"
     CDS             13486..13986
                     /gene="rimM"
                     /locus_tag="VHS06_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX2986889.1"
                     /translation="MDGSYLVIARIVSPQGNRGEVKAEIVTDYPDRFASTVAVYLGLE
                     HRRYELERFRFQDGAVVLKLRGVDTIGDAAKLRGTLVEVPEEDAVSLPPDSYFWHQII
                     GLKVVTVEGDQLGKIDEILETGANDVYVIHGPKGELLIPAIKQVVKEVKLDSGTMTVE
                     LMPELE"
     gene            14024..14806
                     /gene="recO"
                     /locus_tag="VHS06_01795"
     CDS             14024..14806
                     /gene="recO"
                     /locus_tag="VHS06_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX2986890.1"
                     /translation="MAERPRIYRTRGVVLKRTDLGEADKILTIYSYSSGKLRAVAKGV
                     RRPSSKLGGHVDDLSYSDMLLARGRDLDVVTQSQTIDTFRGMREDLWRVSCGYYLTEL
                     VDSFNEDRIENHQLFELLVSSLQRLSQGDDLFTTVHFFELHMLEMVGYRPELTHCLSC
                     RHEIKPETNYFGAILGGVLCPECGRREGTAQPISLGALKVLRYLQRLDAPPSGGLRLD
                     VSTRREVDSTLRLDIEHLLERTLKSNAFLDRLRLEDMVTKPT"
     gene            complement(15074..15505)
                     /locus_tag="VHS06_01800"
     CDS             complement(15074..15505)
                     /locus_tag="VHS06_01800"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2986891.1"
                     /translation="MTHSSRALNGKPPLIYEDGLQSRDFVHVSDVAQALMLAMESQAA
                     DYGVFNVGTGKALTVLNMAELICRRVGPAGLRPSVAMKYRKGDIRHCYADISQLSALG
                     YKPKVAFEDGVRELVNWVTTQSAEDRVEQASEELKKRGLVS"
BASE COUNT         2992 a         4433 c         5184 g         3022 t
ORIGIN      
        1 ttgagataga ccaatgcacc atcccgaggc aacatgcgga agtcgtcggt gagcactatc
       61 acctcgcctc cttgctctgc ggctcggctt ttgatgagtt ctattgcctc tcgaaagccg
      121 tagccggagg ggaagccgga gatgtactgg aagcggtcaa actctactag gtcagcgcga
      181 ctggggtccg tcagcagttg gatgtcgaag gctgcggaag gcgccacggt tgccaggagg
      241 agaagaaggc caatacctcg agaaatgctg tggacatgcg gcaatgataa tcgctcgtgc
      301 actgctcgcg ccccccgttc ccacgtcttg accatcagga gcccgaccat cactaccagc
      361 ggaggaatgc tcggtactag gtaccgactg gtgaacaggt ctttgcccac tagaatcggc
      421 gggacggcga gagaaatcca gcatcccaac aagagcagtg tctctcggcg gcgcgtgcca
      481 aggccccaca ccacagcggc cagtaccagc cacgacagtg gactcggcag gtatgtctgg
      541 agccacgaga tgtaggcagc ggcgttcttc ccccagagcg ctaacggaaa ggtcaggatt
      601 tcagcaggcg tcagcgagaa gtgactcccc ttttcgatta ggttctccgc ttcggggagt
      661 agcaccatga tgccggccaa aagggcgaag agtccgtacc ctactgcgag tgcgagacaa
      721 gcgcgagcaa ggtcgcggcg tttgcgcaca agcaacaggc acagcaatgg tatgggcagg
      781 acgaagagtc ctgtcatctt tacccagaca gcaatcccca tcatcgtgcc aagggctgca
      841 ccgagcagaa ttgcccgcac ttgagtactc ggtcccattg ctatgcgaac tccaagacat
      901 gaggcccata cagatgcagc agcaatggta ccgtccatca agccaatgcg atcgaagaag
      961 actgggtagg gggccagggc ataaagaaac gcggctacca acccagtacg ccacgtatcg
     1021 agccatcgtc ctagtagcag aatccccagg gtagctgcac tacctgctag cgccgagaca
     1081 attcttccgg ctaccaaggg gtctgcaaag aatctcatgg ccaacgccac aagccatatg
     1141 aagaggggtt gcttgccgtc ctcgtggatg ggaatcagaa gtgacatgcg ggttcgctgc
     1201 tcgagcatgt cgtaggccca tcggaggttg ttggattcat ctatgaagat tggcagagac
     1261 gtgatgttcc acacgcgcaa agtcaaagac agcaccacga cgccgaaagg caacagccac
     1321 tttgcagagt atgctggtcc aggaatgatg ctatcacggg gcattagatt gccccgtgat
     1381 gccagattat tgatgctatt cttggtcaac cagagtccct ccaaggtgac tctccgagcc
     1441 tgctatgcgg caatctcgag cgctgaaagg atggcggcgg agaggaatgt acttctgcca
     1501 ttgacggtag agactgtcag aaccgatgat cccagcccgg agcaaaccag tgtcacagcg
     1561 agacacagga cgaagcacag cattttcacg gcgcagtggc tcccgctcgt tgtctttgtg
     1621 tggagatagg cgtggagatg cctttctaga tgccgggcat cgtatcactt tgaagatggt
     1681 aactcaaaac ttggcagggg gggaagggca tatatcccgg caatcgggct accaaggctg
     1741 gcctttcgtg ccggatgagg gaacgtggcc ttacctggtg gcgccactct cttggtgttg
     1801 agccccatcg ccaccattac tcgtctgctg cgagaggcac aatgcttgca acgtgcccct
     1861 aacccttggg tttccccaga gggcaattgg tgacgccgca gcttggcgtc gggggctgaa
     1921 gtaccggctt ttggccggcg aattacccag gatggtgtca tgagagaaca cgccgattac
     1981 ttcactcaga cctccccgga gccgatgacc gaggaggatg aggagactct tgactggctc
     2041 aatagagcgt tggcgggcga tccacgcaca acggacgcca taatcatggt cgaggtacac
     2101 gggggagaag tggtgctgcg cggggatacg caggtgcccg gagtcaagcc cgcggcagag
     2161 gatgtggcct ggggcgtgcc cggcgttcac tccgtggtca atcagatcgt cgcccacggc
     2221 gctgcatccg gcgagtagcc attgaagtag acttgccgat agggtacgct ccttctgcct
     2281 tcggaccggt gatcttggcg gctggaggag actatggaat tctccgatac cctactcggc
     2341 agggccgcgt ccgtgcggga agatgtcttc gcccatcctt tcgtccgcgg tctgggcgac
     2401 ggtactctcc ccgagcccaa gtttcgcttc tatatgtgcc aggactacct ctttctgatc
     2461 gagtactgcc gggtctttgg gttggcgacc gccaaggggg aagatctggc taccatggcc
     2521 cgcttcgccg aactgcttca ctcgaccttg ggggtcgaga tggagcttca ccgacgctat
     2581 gccgcggaat tcggccttag ccggaatgac ctggaggaga cggagattgc tcccgcgacc
     2641 catgcttaca cgagccacct gttgaacgtg gcctggagca gtagtgtggg ttgtatcgcg
     2701 gccagtcttc ttccctgtca gtggggctac tgggaaatcg ggtgccggtt ggcgcagttg
     2761 ccggggttgg agcagaaccg atacaggcgg tgggtagaga gctattccct ctatgagttc
     2821 ggagagttgg cgagatggct tcgtgggctg gtggatgagg tggccgcgac ggcttcgccg
     2881 gcagagttgg gacggatgga gcgagagttc atggcaagca gccgctacga gctgatgttt
     2941 tgggatatgg cctggaaagc ctagcacgct tcttgctacc aagatgaggc gtagaagtct
     3001 gtcgggggga cagccttctc gccacactgg gcgggcggac ccacacgccg gcccagtgtt
     3061 tttttcgcaa tcgttcttct gcgcgtcatc gtcgtccaac tcgatgatag aatggggtga
     3121 taggcgtact ggaggcaggg ttgagggcag ggcatcatag tctctggcgg ctggcgaccg
     3181 actggctccc ggccgttgtg gttatggggt tgatcttctg gctatcgtcg cagtctgtac
     3241 ttcctggacc cgccgacaag ggaatggact tcctcttcaa gaagtccgcc cacctgatgg
     3301 cctatagtgt ccttgcgctg agctatcttc gggcagtgca tgcggcacgt agaccctacc
     3361 tggttgcttt tgtgctcacc gtgctctggg ccatttccga tgagatccac caatccacgg
     3421 tacccttgag ggagcccact ttcagggatg tgctgatcga cgcgaccggg ggcggtgttg
     3481 cgctgtggct ggtgctgagg tcgattgctc acacgcagag tgccgtgggc gaggtgttga
     3541 gggctgccat gaggattgga gcggggacgg gagccggtta gagttctcgt ccccatctct
     3601 gggtgttcag tagacgctag ttggcacgca cgcagttgtg cccctcgggc tcaatggcgt
     3661 ccccacaaag aggacaccgc ggccgacctg cggagatcac cgtctcgatc cgtcggctaa
     3721 gggctctcat ctgtgccgcg gaggcgaggc aactgaaggt cgccggacct tctggatcat
     3781 cctccgcgtc gtggacgaga agcgcaaaca ggtcggagtc gctctcgtat cccaacgaca
     3841 gctgtcccac cttgaactcc accagcggtt ctggggagaa tccttgcgag ggttggtatt
     3901 ccgccgcagg tgctccatac agcttccact ccgggcggtc ggaaaggcga gccaacagtt
     3961 ggtctatggc gacccccaaa gcttgaagct cttccttctc cagccaaagc gcagccgatc
     4021 cctccctggc ggcggccagc aggcaaaagg tccgctgacc gggttcaccg agagcgtcag
     4081 cttctagccg catgaccggt ccgaattcgt gcctggtttg gctcatgtct ggcaattctc
     4141 cagtctccct tatcgatcct aaggatacca cagcttcgga cggactcgca gcttgtcctt
     4201 tggcgtttcc ccccctataa ttcgcccaat ccttttaatt cctttactgg tcaaaggggt
     4261 agcgtgaaac agcttctatc aggcaacgag gccatcgctc tgggcgccta ccacggcggg
     4321 gtctccgtcg cggcggccta cccgggcacc cccagtacag agattctcga gaccatcgct
     4381 cgatacactg agctccatgc ggagtgggcg ccaaatgaga aggtagccat ggaagtggcg
     4441 ttgggcgcca gctacgccgg tgtccgaacg atctgctcta tgaagcacgt ggggctgaat
     4501 gtcgctgccg atcctttctt cggtgccgcg attacaggtg tagtgggcgg tctcgtggtg
     4561 gtcaccgccg acgaccccgg catgcactcc tcccagaacg agcaggataa tcgacactac
     4621 gctcgctttg ccaaggtccc cgtgttggag ccgtcggaca gccaggaagc ctacgagatg
     4681 accaggctcg ccttcgtcct gagcgagcag ttcgataccc ccgttttgtt gagaaccact
     4741 actcgcacct ctcattccaa gtcagttgtg gagtacgacg agactacgat gcccgagcgg
     4801 cgtggtgcca gctttgtgcg gcagccggag aagttcgtca tgatccccgc ccatgcccgc
     4861 cggcgacatc ccctggtaga ggagcgaatt gcgaagctcg aggagtatgc agaggggttt
     4921 gcctacaatc gagtggagat ggccgatgcc tctctaggca tcatctccgc gggtgtagcc
     4981 taccagtatg ctcgtgatgt gatgccgaat gcctccttcc tgaagctggg catgagctat
     5041 ccccttcccg ctaggatgat ccttgagttt gcctcccgag tgaggcggct gatggttgtg
     5101 gaagagttgg accctttcat cgaggagaca gtgcgcgggc tgggagtcca ggtggaaggc
     5161 aagcagttct tcccggcggt gggcgagcta tcggtggagc gagttgagga ggggttcagc
     5221 gcggccggag cggtgcctgc cggtgaagaa atggttcccg cttcgtccga gcctggggcc
     5281 gacaaggagg aacctcttcc cttgaggccg ccggttctct gccccggttg tccgcacacc
     5341 ggtaccttct tcacgctgaa gcggctgggc ttctaccgtg gaaccgtcga cgcaggagcc
     5401 acctctcagg gccagctatt gggcagagtg aagcggaccg ggctggtggt ctcgggggac
     5461 atcggctgct acacccttgc cgtgcttccg cctcttctgg cgttggatgc caccacttgc
     5521 atgggtgcaa gcatcgggat ggcaatgggg atggagaggg cagggctcgc caacaaggtg
     5581 gttgccgtga tcggcgattc caccttcctc cattctggga tcacgccgct actggacgtg
     5641 gtgtacaacg gcggaaccat aaccaccatc atcctggata actcaaccac cgccatgacc
     5701 ggccatcagg aaaacccgag caccgggttt tcggccaagg gcaagccggc cccggctgcc
     5761 gacctggaga agatcgtgag ggggctgggg gtcgaggatg tgaaggtggt aaacgccttc
     5821 gacctggatg ccatctcctc tgctgtgaag gatagtgtgg atcgggatga gccatcggta
     5881 gtgatcgtca ggggagactg tgccgtcaaa gtgaggggcg tgggagccct atccgtcgta
     5941 gacgccgagg catgcaacgg ctgcagcacc tgcctgcgac tcggatgccc ggcgatagcg
     6001 cgagtagagg acaaggccag gatcgatccg accctgtgcg tggggtctcg atgcggcctg
     6061 tgccgccggg tgtgtccgat caacgccatc aaggagcagg tgtcaaatgg ataagctgga
     6121 cttcgtgctg gcgggggttg gcggccaggg gatcgtgctg atgagcgatc tgttggggga
     6181 tgcggccctc gcggcgggct acgatgtgaa gaagtcggat gtccacggga tggctcaacg
     6241 gggtggaagc gtcgtcagtt acgttcgtct gggggcgaaa gtgagctctc ctctgcccaa
     6301 gatgggggag gtggagttcc tagtggcgct ggagaagttg gaggccgcgc ggtgggctcc
     6361 atatctgcga ccaggcggcg ttgccgtggt gaacgatcac gccatctttc ccctctctgt
     6421 cagcgcgggg gccagcagct atccctccga tgagcaggtg gtggccactc tgcgggagcg
     6481 gacggagcgg atctaccaga tcaaggggcc tgagatcgcg gtgggcctgg ctaaccccaa
     6541 ggtgctcaat gtggtcatcc tcggcttcct ggccgcattg ttgccgatcc cgtgggatat
     6601 ttggacggcg accgtcgaac accgtgtgcc gccgaagtac cgtgagttga atcttcgcgc
     6661 cctagaggcg ggacgggcag cagcccgcca gtaagtcgac gcgtggagta gtgtggcggc
     6721 gtgacccagg gtaggacagg cggcgcgagc gagcgtagcg tcgcttccag agcatgggct
     6781 agatcaccac ggtggttatg gcccaaatat gtgcgttgcg tagggcgggg tcttgtgccc
     6841 cgccattagc gagatcgtaa tcacctgctg gtcgtgggca cactgtggga ggcggggcac
     6901 aagcggagcg ctgtgctcgc tcgcgctccg tgccctacga gacgtcccag gtggcctcag
     6961 tcgggtaccg cgacctgtga agccgagcgt tcacggactg gctccctctc aagcggaggc
     7021 gatccatcca gcctgaagac agcgggcgaa cgcgagacgc ttcttctaga tcgaaagtga
     7081 gcagggcaac gccctgccct ttgccgtctg taacgcaggg cgagccggct tgtccgacgt
     7141 ttgtcgcgat gcgtcgagtc ggttctcgat ttcgcatttg aggggaggta tcgggatagt
     7201 ggcgtactgg gatccggaaa gggagacgat ggcgagggcc gacatggagg ccctgcagtt
     7261 ggagttgctt cagaagaagg tggcggaagt atacgaaagg gtgccattct accgggccgc
     7321 ctttcaggag cgcaaggtta ctccgggtga tgttcgctcc ctcgcggatc tggccaagct
     7381 cccctttacc gacaagacgg cctttcggga caactacccc tatgggctgc tggcccgtcc
     7441 gcgcaaagag gtagtgagga ttcacggttc cagcgggacc accgggaagc ctatagttgt
     7501 ggcctacact cggaacgatg tggacctgtg ggcaaacatg gtggctcgct cgttggccat
     7561 gggcggagtg accgacgagg atgtggtgca catagcgtat ggctacggcc tgttcaccgg
     7621 cggtcttggc attcactatg gtgcagagcg attaggcgcg acggtgctgc cgatgtccag
     7681 cggcaacacc aagcgccaga taatgctgat ccaggatatg gggagcactg ttattgcctg
     7741 cacaccgtcc tattctctct tcctggccga gaccgccctg gagatggggt gcgaccttcg
     7801 agactccagc ttgagggttg ggttcttcgg cgccgagccc tggtcgtatc ggatgcggga
     7861 ggagatagag gcgaagctgg ggatcctggc actggacatc tacggcctca gcgagatcgt
     7921 tggcccgggt gcggcgcagg agtgtcccca caaacaggga atccacatct ttgaagacca
     7981 cttcctgcct gaggtgatcg accccgtaac cgaagaaccg ctccctgatg gtgagaaggg
     8041 ggaattggtc attaccaccc tgacgaaaga ggcgttgccc gtgatacgct atcgcaccag
     8101 ggatatcacc agcctgcacc gtgagaagtg cgcgtgcggc cggacgttgg tgcggatgga
     8161 taaggtgatc ggtcgcaccg acgacatgct gatcgtacgg gggatcaacg tcttcccgtc
     8221 gcagatcgag tcggtgctgc tggcggtgga gggggtcgaa ccccactacc ggatcgtggt
     8281 ggaccggcag aaatcgttgg acgacctgga gatccaggtc gaggtatccg aggcggtgat
     8341 ctccgacgaa gtcggacggc tggagcggat cggtcgaaag gtccgagcgg agttggagag
     8401 tgtgctgggg atctccgcac tggtgaagct ggtggagccg ggcaccatcg ttcgcagcga
     8461 gggcaaggcc aagcgagtag tagaccggcg agagctgtag cacccagaac ccagaactca
     8521 gaacttcggt ggtggaggga ctgccatgag aatgaagcag gtatcggtat tcgtggagaa
     8581 tcgacccggc cggctgaagc tgttgctcgc cgccctggat aggatggagg tcaatatcag
     8641 ggcgctgtcc attgccgaca ccgccgactt cggcatcgtg cgaatgatcc tgtcggacac
     8701 ggagaaggga atcgaggcgg tgaagggggc aggatttgcc gccagcacca acgaagtgct
     8761 ggcggtggat atcccggatc agcccggagg cctgctccat acggtggcgg agccgttgtc
     8821 caccgccggc gtgaatatcg agtacgtata cgccttcgtg gacagcccgc tggataaggc
     8881 ccgggcagtg ttgaaggtag gcgacatcga cgctgcggag aagttgatag gcaaatgagc
     8941 gggagcagct tccagggaag actactggtg gtcgatctgg ggtccaggag cgcccagacc
     9001 ttgccactcg acagttccta ttgccggctc ttcctcgggg gcgtggggct tgccactcgc
     9061 ctgctgtggg actactccca cccgggggtg gatccacttt cgccggagaa cccgatggtt
     9121 ttcgctaccg gtccgctgac cggtaccatg atgcccgcgg ccagcaagta tgccatagcc
     9181 acgaaatcgc ccctcaccgg cttcatcggc gattcccttt cctccagcgg gtggccgttg
     9241 gcgttgaagc gcgctggata cgatgcgatg gtcgtactgg gcgccgcgga agagccgacc
     9301 tacctcttta tcgacgacga gcgagtctac ttcaggcgtg ccgtgtccgt ctggggcggc
     9361 gagtgctggg agaccgagca gcggatcagg caggatctcg gcgatgaaag cgtgcaggtg
     9421 gcctccatcg gagtggcggg cgaacggctg gttcgctacg cctgcgtcgg caacggtttt
     9481 ggacggcagg caggacgaac cggacctggc gccgtaatgg gttcgaagaa gctgaaggcg
     9541 atcgcggtgc gtgggaccaa gaccgtagag gtgacggacc ccgccgggct gcgagatggc
     9601 tgcgtcgatc tttaccatcg tagtcagggc gcggccaccg agaagtatcg cacactgggg
     9661 acgccgggca acgtgctagt tttgaacaag ctggccgcgc tgcccacccg caacttccgg
     9721 cagtccacct ttgagggcgc ggagcgggtc agcggggagt ggctccagca gcgccatctg
     9781 ggcaaggtgg tcgcttgcgc gggctgctcc atcgggtgcg accacatcta tcggatatcc
     9841 gaggggccaa gtgcaggtga ccaggtggcc atggactacg agtccctcta cgcgctgggg
     9901 ccgctgtgcg gtgtggacga cgccaacgcc atcctgaggg cgtccgagct gtgcgatcgg
     9961 tacggcacag acacgatgag caccggcgtg tccatcgcat gggcgatgga gtgcttcgag
    10021 cgagggctaa tctccaggga gatggccggc gggctcgacc tgcgcttcgg caacggtgat
    10081 gccctggtga ccatggtgga gcaaatcggg aagcgccggg ggcttggacg gttgctgggc
    10141 gagggggtca agcgggccgc ggcagaggtc ggcggcggtt cggagcgctt cgctatgcat
    10201 gtgaaggggc tggagatgcc cggctacgag cctcgcagcc tcaagacgct tgccctgggg
    10261 ctggcggtcg gcaccagggg gggctgccac aacaggtcgg ccgcatacga ggtggacctc
    10321 tccgccaaag tagaccgctt gaacggcgag cggagtcgtg gaccgttggc catggcgcag
    10381 gaggatttcg cggcgacact ggactccctg gggatctgca agttcctgcg caagtgcttt
    10441 gacgccttct atgccgaaac gtcggcgctc ttccgtgccg gtaccggcct ggatatgacc
    10501 accgaggaac ttcagcaggt gggcgagcga gtctccaatc tgaagaaggc ctacaacgtt
    10561 cgggagggct ggaaaccgga ggatgactgg ctgcccgaca ggctcttcga cgagccgctt
    10621 ccgaccggcg tggccaaggg gacggtgttg acccgcgagg agttgaggga gatgatccag
    10681 ggctactacg atgcccgcgg ctggacggct gagggacaga ttccggcgag caagctggcg
    10741 caactggggc ttggcgacct ggcggccggc gacgagaagg gggctacgga tgcagcgacg
    10801 gctgatactt tgtgatccaa ccctctgcac cgggtgcagg ctgtgtgagt tcgcgtgcgc
    10861 cctggagaag acggggagct ttgatctgga gctgtcgcgg attcgagtgg cctcgccaca
    10921 gcccgacagc gtgatcagca tctcctgcca gttgtgcgag agcgctccgt gtgtggaggc
    10981 atgcccgcgg gatgcgctca caatcaggtc cgacgacggc accatagcgc tgaacaaggc
    11041 gctatgcagt gggtgcggat ggtgtatcga ggcgtgcgat tttggagcta tcgccctaga
    11101 tcgaagcacc aagtcggtgg ctatctgcga cctgtgcagg gagactgcgc agccgaggtg
    11161 cgttgagagt tgccccaaga aggcgctgtc gttggcaccc aagtctaccg ctgcggggcg
    11221 ctctcgaaag aaggccgccg agcagttgct caggcgcgcc taggagcatc ctggttcggg
    11281 gatggctgga gggcagggcc ctgtgccgtg ccgcccggtg gccttggcga cgcgatggcg
    11341 gcagggcaga gcgccccgcc ctactcagat gtggtgtgtc gctcgactag atgggcggcc
    11401 agggcacgtt gcggttggga tccaattgcg gatagtggcg caccttcagg atccggtcga
    11461 ttcggcggaa gaacgccttg atctcggcag gttccaggtc cggctccagc gcctcccgca
    11521 gtttctggct gcgaccctca tcttccctca gcgcctgcat ctgctcgagc agtcgctgcg
    11581 gtaccggctg accgcaatag tcccacagta tcgttcgcag cttcggctcc acgtggaagg
    11641 tgagtccgtg gtcgattgcc cacaggcgac cgtcctcccc gagcaggcag tggccccctt
    11701 tccgatcggc gttgttcacc agcaggtcga agagtgcgat ttgcatcaga tcgcggcggc
    11761 gcttgtggcg aaaggagaag tagtcggcgc catcctggat ggggacgtac agttggacgc
    11821 tgccgatgcc gaaggggccg tcgcggatga cggtgaccgg aatgttgggc cagccgagat
    11881 gccggctgac gacgtaggcc gcgtgttcac gttgatggag ggtgccacgc gggaaatccc
    11941 acagggggcg ctcgccgcgc tggggcttgt agacggccag gaaccgtccg tgaccgtctg
    12001 cggaaatcag cacgaagaag gtgtagttgc tgccatcggg cacgacctcg tgagcaagga
    12061 tctccccatg catcagcagg gaggctgcct cctcgggcga taccgcaacg actcgctcag
    12121 acgtggaggg tgaagactcg ccttcgccct tcgtgcgctc tccacgtggc cgctgtcctt
    12181 catgatgtcg ccgtgccaag tttgcctcag ccaacctttc cgaggccggg gtttggccct
    12241 gccaggattt ctacataata tggtaaccgg ccaggcgcaa accctcaacc tgtccggcct
    12301 agaagtgcac cacagcgcgg atagaccgca ctcaggattg aggaaatcgg acgtcctaca
    12361 tcatgccgtg aggagacggg gcgcccaata ccatgttagg ctgagcgcag cgaagcatct
    12421 cctcgcgctg tgagagtcac gaagagatcc ttcgctgccg ttcaggatgg cacccaattg
    12481 ccgcgcaggg agcagcgaca cggctgggcg gcctagctcg gccatgtcac agctctatgg
    12541 ttgccccaag gacactagac tgttgccggc gcgggcggga ttatagccca gcagacgaag
    12601 tagagcagga cccctgggaa gaacatcgtg accattgccc ccaggaccca gattaccctg
    12661 atgatggtcg ggtccacgtc gaagtactcg gccatcccgc cgcatacacc cgcgattatg
    12721 cgctcacgcc gggatcgata gagacgtttc atctttccac ctccatctac ttaccgggca
    12781 aggggcgtgc ccgagaggtc ggtgtttgcc gtacggggcg cggtcaacta taatatgcgc
    12841 atcgctagta aagttcggcg aggccatccc taagtgcgcc cttcgcgggc cgagggtagc
    12901 ctcgctaata gtgttagcct cgaaaggaga ggggatgatc aagatccggt tgcggcggac
    12961 cggcggccac aagcagccca gctatcgaat tgtggtagcc gatgtcaggg ctcccaggga
    13021 tgggcagtac atcgacctga ttgggaacta caatcctcgc actgagcccg cgaccatcgt
    13081 tgtggatgcc gagaaggcga acaagtggtt ccagcagggc gctcagatga ccgagacggt
    13141 tgagaagctg tttgcgcggg cgggtgttct ggagcaggtc aaggcgcgca aagcgtaatc
    13201 gatgccctca ttaaggacgg actggtagct atgaaagagc tggtggagtt tatcgcccgg
    13261 tctctggtgg atacccctga tgcggtcaag gttacggaga tcgagactga tcagatgacc
    13321 acctacgaga tcgaggtgga tcagcccgac atcggcaagt tgatcggacg ccagggccgc
    13381 atcgtcaagg cgatcaggtc cgtagtcagg gctgccgcag ccagacaggg caagcgggtg
    13441 gccgtggagg ttgtctaacg aatccctatc cccgggcgca taaccatgga cggtagctac
    13501 ctggttatcg cccgcatcgt ctctccgcag ggcaacaggg gcgaggtgaa ggccgagata
    13561 gtgacagact atccggatcg cttcgcctcc accgttgccg tctacctggg gctggagcat
    13621 cgccgctatg aactggagcg tttccgattc caggacggcg cggtggtgct gaagctgcgg
    13681 ggcgtcgaca ccataggtga cgcggcgaag ctccggggta cgcttgtaga ggtgccggag
    13741 gaggatgccg tgagtctccc tcccgacagc tacttctggc atcagatcat aggtcttaag
    13801 gtggttaccg tcgagggtga tcagttgggc aagatcgacg aaatcctgga gaccggcgcc
    13861 aacgatgtct acgtgattca tggccccaag ggtgaactgc tgatcccggc gatcaagcag
    13921 gtcgtcaagg aagtgaagct ggactccggc acgatgactg tggagttgat gccggagctc
    13981 gaatgatagg gcgagctctt ctcccctcta gaaagaccgt ccgatggcag agcgtccgag
    14041 gatctataga accaggggcg tggtgctgaa gaggacggac ctcggcgaag ccgacaagat
    14101 cctcaccatc tacagctaca gcagcggcaa actgcgcgct gtagcgaagg gcgtgcgccg
    14161 acccagcagc aagctggggg ggcatgtgga cgatctctcc tactccgaca tgctgctggc
    14221 cagggggcgt gacctggacg tcgtgacgca gagccagacc atcgatacct tccggggcat
    14281 gcgggaggac ctctggcggg tcagctgcgg ttattacctc acggagctgg tggatagctt
    14341 caacgaagat cggatcgaga accaccagct cttcgaactg ctggtgtcca gcctgcagcg
    14401 gttgtcgcag ggggacgatc tattcaccac ggtgcacttc ttcgagctgc acatgctgga
    14461 gatggtgggc tatcggccgg aattgactca ttgcctctcc tgccgccacg agatcaagcc
    14521 ggagacaaac tacttcggcg cgatcctggg cggtgtgctc tgccctgagt gtggtcggcg
    14581 cgaggggacg gctcagccga tttccctcgg tgccctgaag gtgctccgct acctgcagcg
    14641 gttggatgcc ccaccctcgg gcggactcag gttggatgtg tctaccaggc gggaagtcga
    14701 ctccacgctg cggctcgaca tcgagcactt gctggagcgc acactgaaat ccaacgcatt
    14761 cctggatcga ttgcggctgg aggatatggt caccaagccc acgtagttgg gcggtgtgct
    14821 gtggtagctc ccgcatggag tggatcggct tgccgcgttg aggcgacctg aacggcatgt
    14881 agggcggggt cttgtgcccc gcctcccaca gcgtggatgc gacccccatg gtgattcctg
    14941 gcgacgctcc gagcggggca caagaccccg ccctacgtca cgatcaacgc ggccagctac
    15001 ctcatggccg ccattagccg aagtgatcgg cggggcgtag gcgggatgtt gcgctcaccc
    15061 gcataacgca ctcctacgag acgaggcctc gtttcttcag ttcctcggag gcctgctcca
    15121 cccggtcttc cgcgctctga gtcgtcaccc agttcaccaa ctcgcggacc ccatcctcga
    15181 atgccacctt cggcttgtat ccaagggcgc tgagctggct gatatcggcg tagcagtggc
    15241 ggatgtcgcc cttgcggtac ttcatcgcca cgctggggcg tagccctgca gggcctactc
    15301 tccggcaaat cagctcggcc atattcagga cggtcagcgc tttgcccgtg cccacgttga
    15361 acaccccgta gtctgccgcc tgggactcca tggccagcat cagtgcttgt gctacgtcgc
    15421 tcacgtgcac gaagtcgcgg ctttgcagtc cgtcttcata aatgagtggg ggctttccgt
    15481 tgagcgcccg cgaggagtgc gttatgcggg tgagcgcaac atcccgccta cgccccgccg
    15541 atcacttcgg ctaatggcgg ccatgaggta gctggccgcg ttgatcgtga cgtagggcgg
    15601 ggtcttgtgc cccgctcgga gcgtcgccag g
//
LOCUS       DATALS010000083         4722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_409134, whole genome shotgun sequence.
ACCESSION   DATALS010000083 DATALS010000000
VERSION     DATALS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409134"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VHS06_01805"
     CDS             <1..1459
                     /locus_tag="VHS06_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2986892.1"
                     /translation="ARVISATIARSPATEARSLRATGREVSKSMPPDGGLEAGMCRPV
                     GERATGRAGSLRAIVSTGRTPVKPPICITMIVGEGGMVASENVSVKDEATMTPGSVRE
                     YAEAMRSRYVTAKTRAEKGRILTEFCQTTGYHRDSAIRVMWHSAAPSGKRRGRPRTYG
                     HEVSEALKQLWEVADRICGKRLHPFLPELLESLERAGEIVVEKRIRCQLLGLSSATMD
                     RLLSPCRRSLLRQPYVPSHSSSAIQALVPIRTFGEWEGVEVGSMQMDLVLHCGESLDG
                     FYLTTLVAVDVATGWAEVRVVWGKGKERVGGAAAHIRKALPFALREMHTDNGGEFLND
                     ALYPWARRFGIGFTRGRPYKKNDQAHVEQKNWSLVRQRVGYDRYSSKAAYVQLEKLYD
                     SLRLYANFFQPLRKLVGKERVGSKVIKQYDRARTPYQRLLETEGLSQDKRAEVEKLYR
                     KLNPAKLKREIDQSLEELWKLADKPGQAKAKTACG"
     gene            1751..2476
                     /locus_tag="VHS06_01810"
     CDS             1751..2476
                     /locus_tag="VHS06_01810"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX2986893.1"
                     /translation="MKGVAMGLRAGVGLLAVLACLAAFPTFTEAETLWQGTVAGDQAN
                     VRSAPAASAPVVDVLDAGTPVTVATWVSGDEVQPTNNTWAKLTSGGYVYSALIHKDQP
                     KAAPPVPGGPQYQGKWLDANLTQQVITAYVGSTPVHVAVMSSGRPDYPTPIGSFSVLR
                     RVANETMDSTSVPWVRDHYRLENVLFTQYFTSMGAAIHEAWWKTPDSFGIPTSHACIG
                     LPFDEAQWFWNWATEGTPVYVHQ"
     gene            2580..3917
                     /locus_tag="VHS06_01815"
     CDS             2580..3917
                     /locus_tag="VHS06_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX2986894.1"
                     /translation="MQQRRSEGRFHEAAASKSGQGRRPLLQVVRLDRYESGTASSMDA
                     RETLRHLGPGIISGISDLDPTTVATLAIIGSALGYQLLWVMTLLLPMLITVQVVSARI
                     GVATQKGLEAAIRERFGMVWAVAAMCLAVSVNILTLAADLEGGAEALQLLTGVAWRWF
                     VVPIALVLGALLLFGSYSVIQRVLRYVLVVFGAYIIAGFMARPDWLSVIYHALVPSLS
                     LNPAYVAGVLALLGTVLTSYVYFWEAIEAQEERRPIGDIWLVEMDAAVGIVAALTLSA
                     FIILTTAATTGTTGTPVHSAEEAARALVPLAGPLAGEIFALGLLASALLAVPVLASTT
                     AYILSEAFQWGGGLDSREATAHPFYAVLLGSLAIGVAVALAGVEPFQILFIASIAGGL
                     GAPVLLALMIMVARDPEIMRDHPISGALSSLGWLTTVVLGGASILYVIQQFLG"
     gene            4098..>4722
                     /gene="pxpB"
                     /locus_tag="VHS06_01820"
     CDS             4098..>4722
                     /gene="pxpB"
                     /locus_tag="VHS06_01820"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEX2986895.1"
                     /translation="MSDVTFKPATIVPAGDAAALVEFADEISLDVNAQVHALARAIEA
                     TRPKGIGETVPAYSTLLIQYDPLATTLEDVGLLVQRLASGEAEAAFGAARLREIPAVY
                     GGAYGPDLPSVAQRLNMTEEEVVRLQTSVDYTVYMLGFAPGHPYLGGLPPQLVLPRLQ
                     SPRPLVPRGSVAIANQATVYAMDTPGGWHLIGRTAVRLFTPEQDPPTY"
BASE COUNT          879 a         1454 c         1570 g          819 t
ORIGIN      
        1 ggcgagggtg atcagcgcca ctatcgccag atcgccggca acagaggcgc ggagcctccg
       61 ggcgaccggt agggaagtgt ccaagtcgat gcctccagat gggggccttg aggccgggat
      121 gtgccgcccg gtcggcgaaa gagccacggg gagggccggc agccttcggg cgattgtaag
      181 caccggccgg acgccggtca agccgccgat ctgcatcaca atgattgtcg gtgaaggggg
      241 tatggttgca tcagaaaacg tgtcggtgaa ggacgaggca accatgaccc cagggagcgt
      301 tcgagaatac gccgaggcaa tgaggtctcg gtacgtaaca gcgaagacga gagcggagaa
      361 gggacggatc ttgacggagt tctgccagac gacgggatac cacagagact cggccatcag
      421 ggtgatgtgg cattctgcag ccccgtcagg caagcgtcgc ggtcgaccgc gaacatacgg
      481 acacgaggtg agcgaggcgc tcaagcagtt gtgggaggta gcagacagga tctgcggcaa
      541 gaggcttcat ccgttcctgc ctgaactgtt ggagagcctg gagcgtgccg gtgagatcgt
      601 tgtggagaag cgtattcgct gccaactgct tggtctcagc tcggcgacga tggacaggct
      661 gctcagcccc tgccgccgct cgttgctgcg ccagccctat gtgcctagcc actccagttc
      721 ggccatccaa gcccttgtgc cgatacggac ctttggggag tgggaagggg tggaggtagg
      781 gtcgatgcag atggacctgg tgctccactg tggagagagc ctggatgggt tctacctgac
      841 gacgctggtg gcggtggacg tggcgacggg atgggcggag gtgcgggtag tatggggcaa
      901 agggaaggag agagtggggg gagctgccgc ccacatccgc aaggcgctgc cctttgccct
      961 gcgagagatg cacacggaca acggtgggga attcctcaac gacgcactgt atccctgggc
     1021 tcgtcgcttc ggcattggtt tcacgagggg caggccctac aagaagaatg accaggccca
     1081 cgtggagcag aagaactgga gtctcgtgcg acagcgagtg ggttatgacc gatacagcag
     1141 caaggcagcc tatgtccagt tggagaagct gtacgactcc ctgcggctgt acgccaactt
     1201 cttccagccc ttgaggaagc tagtcggcaa ggagagggtg ggcagcaagg tcatcaagca
     1261 gtacgaccgc gccaggaccc cctatcagcg gttgttggag accgagggcc tgtcccagga
     1321 caagcgggcc gaggtggaga agctgtacag gaagctaaac ccagccaagc tgaagagaga
     1381 aatcgatcag tccctggaag agctatggaa gctggccgac aagcccggtc aggccaaggc
     1441 aaagacggcg tgtggataag tggaaagctc tccgcaagct ccgaccttcc cacttatcca
     1501 cacgcccgac tacgacgacg aaagaggtgc tgcatgactc aactagccgt tgccaaacaa
     1561 agcccttcac cgacaccaat tctgatgcag ctacccgtcc ttcaccgaca gtatctattg
     1621 atgcatcacg gccgcggcgg gatcggcagg ggaagggggt tgtccaactg gcacccttct
     1681 tgcttttctc cggcgctctt tcgggaactg ggacgcaaag gatgcggccc gatacgaaag
     1741 gaggcatctg atgaagggtg tcgcgatggg gctgcgcgcc ggtgtcggcc tgctcgccgt
     1801 gctggcgtgc ctggcagcat tccccacctt taccgaagcc gaaaccctct ggcaggggac
     1861 cgtggccggc gatcaggcca acgttcgcag cgctccggcc gcgtctgctc ctgtggtcga
     1921 cgttctggat gcgggaaccc cggttacggt agcgacctgg gtatccggcg acgaggttca
     1981 gcccaccaac aacacctggg cgaagctgac gtccggcgga tacgtctact cggcgctgat
     2041 ccacaaggac cagccgaagg ccgcgccccc ggtgcccggg ggtccgcagt atcaagggaa
     2101 gtggttggac gccaacctga cgcagcaggt cataaccgcc tacgtcgggt ctaccccggt
     2161 acacgtggcg gtgatgtcgt ccggtcgccc ggactatccc acccctatag gcagcttcag
     2221 cgtgctgcgc agggtggcca acgagaccat ggatagcacg tcggtgccgt gggtcaggga
     2281 ccactacagg ctggagaacg tgctcttcac ccagtacttc acctccatgg gtgccgccat
     2341 acacgaggcg tggtggaaga cgcccgacag cttcggcatc ccgaccagtc acgcctgtat
     2401 cggactgccc tttgacgagg cgcagtggtt ctggaactgg gccaccgagg gcacgccggt
     2461 atacgttcac cagtagatag ggaggggcgc ggcatcagtc cgccgcgctc caagccccac
     2521 ctgggcacga tatctgcctc tcgaatttct ctctcatcaa cgtcgataga ggtgcaacga
     2581 tgcagcagcg acggagcgag ggtcgtttcc acgaggccgc cgcgtcgaag agcggacagg
     2641 gccgaaggcc gctgctgcag gtcgtcaggc tcgaccggta cgagtccggc acggcctcga
     2701 gcatggatgc ccgcgagacc ctccgccatc tcgggcccgg gataatctcc gggatctccg
     2761 acctggaccc gactaccgtc gcgacgctgg ccatcatcgg ctccgccctc ggctaccaac
     2821 tgctgtgggt catgaccctt ctcctcccga tgctgatcac ggtccaggtg gtgagtgccc
     2881 ggatcggcgt cgccacccag aaggggttgg aagccgcgat ccgggagcgc ttcgggatgg
     2941 tctgggccgt ggccgcgatg tgtctcgcgg tctcggtgaa catcctcacc ctcgccgcgg
     3001 acctggaggg cggcgccgag gcgctgcaac tcttgaccgg cgtcgcctgg aggtggttcg
     3061 tcgtacccat cgcgctggtg ctcggagcgc tgctgctgtt cggcagctac tcggtgatcc
     3121 aacgggtgct gcgatacgtc ctggtcgtct tcggcgccta catcatcgcc ggcttcatgg
     3181 cacggccgga ctggctctcc gtgatctacc acgcgctggt acccagcctc tcccttaatc
     3241 cggcctacgt cgccggcgtg ctggcgctgc tgggtaccgt tctcaccagt tatgtctact
     3301 tctgggaggc catcgaggca caggaggagc gccggccgat cggcgacatt tggctggtgg
     3361 agatggacgc cgccgtcggc atagtcgcgg cactcacgct atccgccttc atcatcctca
     3421 ccacggcggc cacaacgggg accaccggca ccccggtgca ctccgcggag gaggcagcca
     3481 gagcgctggt gccgctggca ggtccgttgg cgggtgagat cttcgccctc gggctgctgg
     3541 cgagtgccct gctggccgtc cccgtcctgg cgagcaccac ggcctacata ctatcggaag
     3601 cgtttcagtg gggcggaggg ctggatagcc gcgaagcgac ggcacacccc ttctatgcag
     3661 tgctgctggg aagtctcgcc atcggcgtgg ccgtcgcgct cgcaggggtg gaaccgttcc
     3721 agatcctgtt catcgccagc atcgcgggcg ggctgggtgc gccggtgctg ctggcgctga
     3781 tgatcatggt tgcccgagac ccggagatca tgcgagacca cccgatcagc ggcgccctgt
     3841 cctcgttggg ctggcttacg acggtcgtgc tgggtggagc gagcatactg tacgtcatcc
     3901 agcagttcct gggatgacgg accggttttg ctatactagg ccctgcgttc tgtagggcga
     3961 cgtacgcatc gccaaaccga gagctgagtg ctgggctgtc tcaactaccg ctcgatgagc
     4021 atggcgaggc atcgggtccg caggtagatc tcgcctacaa aaccgggatc acggatagag
     4081 cagatggtgg agtctcgatg agtgacgtaa ccttcaaacc ggcgacaatc gtccctgccg
     4141 gtgacgcggc tgccctggtc gagttcgccg acgaaataag cctggacgtc aacgcccagg
     4201 tccacgccct cgcaagagcg atcgaggcca cccgaccgaa gggcatcggg gagacagttc
     4261 cggcctactc caccctgctg atccagtacg acccgctcgc gaccaccctg gaggatgtcg
     4321 gactgctggt tcagcgtctc gcctcgggtg aggcagaggc ggccttcggc gcggcgcgtc
     4381 tgcgagagat cccggcggta tacggtgggg cctacggacc ggaccttcca tcggtcgccc
     4441 agagactgaa catgaccgag gaagaggtgg tgaggcttca gacgagcgtg gactacacgg
     4501 tctacatgct cggcttcgcg cccggccacc cctacctggg cggccttccc ccgcaactgg
     4561 tgctgccgcg actccagagc ccgaggccgc tggtacccag gggctcggtc gccatcgcga
     4621 atcaggcgac ggtctacgcg atggacaccc ccggaggatg gcacctgatc ggtcgcaccg
     4681 ccgtccggct cttcactccg gaacaggacc ctcctaccta cc
//
LOCUS       DATALS010000084         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_187041, whole genome shotgun sequence.
ACCESSION   DATALS010000084 DATALS010000000
VERSION     DATALS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187041"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VHS06_01825"
     CDS             <1..220
                     /locus_tag="VHS06_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEX2986896.1"
                     /translation="HLLGASGGLESVATVLSMRDGFIHPTINLEAPDPECDLDYVPNR
                     GREATIKVALKNSFGFGGQNACLVFKRF"
     gene            398..1117
                     /locus_tag="VHS06_01830"
     CDS             398..1117
                     /locus_tag="VHS06_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX2986897.1"
                     /translation="MRTVVNERYVGSRMKIGNYSHFASMALLVIGFVLSLMTGTFGQE
                     VIFVSYVCLIGAFLLLSYGRRFTRRWGPRFRQDQWLVPALKGVDANYTMFNYAAPGLP
                     DHLIVGPSGLYVLVPRPNGGTVQFQDGRWSRGSAGSTIFRGLAEGGLGNPMRDAQIAL
                     QELADYLRKNGSEELISGLEARPVIVFTNPGVDLVVKNPPLPIVKTRDLKSLFRRAKS
                     SLEPNKIEELKQVLGQGVSAQ"
     gene            1114..1572
                     /locus_tag="VHS06_01835"
     CDS             1114..1572
                     /locus_tag="VHS06_01835"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX2986898.1"
                     /translation="MSEDEELVRQPGSQNCFVCGKRNPIGLNVSFYQKGNQVFTHFTP
                     GPEHQGYPDRMHGGIACTLLDETVGRAGFISGCWTFTAKFEVRYRKPVPLGREITVVG
                     EMVRDRGRMIEAKGQLLLDDGTVAVEASGLFVKLKPDELEAIEEEIGEME"
     gene            2001..2306
                     /locus_tag="VHS06_01840"
     CDS             2001..2306
                     /locus_tag="VHS06_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2986899.1"
                     /translation="MDYARKEILLDGEQVAVTPAEYRLLACLARHLGKVVSSSELLKE
                     MSGYDCSEQEAQEIVKVHVSRLRSKLDKDPGKASYLLNVRGFGYLLERRISAARSMA"
     gene            2362..>2691
                     /locus_tag="VHS06_01845"
     CDS             2362..>2691
                     /locus_tag="VHS06_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986900.1"
                     /translation="MKPPLPSARVNLLVLLLALSLLLSVTHPPISQIPLAWASPDQSE
                     TPAAQSDRQDFVPGELLVKLRGESPELPLSLIQSLGYPISVLEKMEGLGVLRLSVPAG
                     REMESIQL"
BASE COUNT          523 a          797 c          839 g          532 t
ORIGIN      
        1 ccacctgctc ggggcgtccg gagggctgga gtcggtggcg acggtgctga gcatgcgaga
       61 tggcttcatc caccccacca tcaatctgga ggcgccggat ccagagtgcg atctggacta
      121 cgtcccgaac cgaggcagag aggccaccat caaggtggca ctgaagaact ccttcggctt
      181 cggcggccag aacgcctgcc tggtgttcaa gaggttctga gttctgagtt ctgggttctg
      241 agtgtcgaga gagtgcacga ggattgcatg cactcctcgg cgagcgcagc agaagacttg
      301 cttcggttat gatcatacgt gcataggcgg ctctccggca aggctaccct gatcaagaca
      361 gggcgtcgtg ccgataatct aagaactggg tgattcaatg cgaaccgtgg tgaatgagcg
      421 ctacgtcggc agccgaatga agatcggcaa ttactcccac ttcgcgtcca tggcgttgct
      481 ggtcatcggt ttcgtgctct ctctcatgac cggcaccttt ggccaggagg tcatcttcgt
      541 ctcctacgtg tgccttatcg gcgccttcct gctgctcagt tacggccgca ggttcacccg
      601 ccgatggggg ccgcgtttcc gccaggacca atggctggtg cctgccctta agggtgtaga
      661 cgccaactac accatgttca actacgccgc tcccggccta ccggaccacc tgatagtcgg
      721 gcccagcggc ctgtacgttc tggtgcctcg acccaacggt ggaaccgtcc agtttcagga
      781 cggtaggtgg tccaggggca gtgccggctc gaccatcttc cgcgggctgg ccgaaggcgg
      841 gttgggaaac ccgatgcggg atgcacagat tgcgctgcag gaactggctg actacttgag
      901 aaagaacggc tccgaggagt tgatctctgg gctggaggcc cgaccggtca tagtgttcac
      961 aaaccccggt gttgacctgg tcgtaaagaa ccctcccctg cccatcgtca agaccaggga
     1021 tttgaaatcg ctcttccgta gggccaagtc cagcctcgag ccgaataaga tcgaagagtt
     1081 gaagcaggtc ctcgggcaag gagtatcagc gcagtgagcg aagacgagga gttagttagg
     1141 cagccgggtt ctcagaactg cttcgtgtgt ggtaaacgga atcccatcgg cctcaacgtg
     1201 agcttctatc agaaggggaa ccaggtattc acccacttta cgcctgggcc ggagcatcag
     1261 gggtatccgg accggatgca cggtggcatc gcctgcaccc tgctggacga gactgtcggc
     1321 agggcaggat tcatctcagg ctgctggacc ttcaccgcca agttcgaggt gagataccga
     1381 aagccggtac cgctgggtcg agagattacc gttgtgggtg agatggtccg ggataggggg
     1441 cggatgatcg aggcgaaggg acagctgctg ctggacgacg gcacggtcgc cgtggaggcg
     1501 agcggactct tcgtaaagct gaagcccgat gagctcgaag ccattgagga agagatcggc
     1561 gagatggagt aggagcggat tgggagcgat gcccgatgag attctggctc tgctgagcga
     1621 tcccgaagcc cgcggcgtcc tttcgactgc tgcgggcagg ctcggcctca cattggtctc
     1681 gtccgatggc gaaatgctgg cgcccaacgg gcttgcagac cacagcagag cgagactggc
     1741 cgtcctggat ctcgatgccc cctcggttac cggcgtcgaa tcgtgccgaa agatccgagc
     1801 cttctccgat atccccctga tagccctcgg caaccgcacc gacaagagcc ggatcgtgga
     1861 ggccctcctc tccggtgctg attgctacct tccaaagccg ctggattccg agttactgga
     1921 agcccacatg tcggttctaa tccatcgatg gtcccccccc agtaacggaa tcgcagaccg
     1981 tctccgttcg agggctaacc gtggactacg cccgcaagga gatcctcctg gacggggagc
     2041 aggtggccgt aacgccggcg gagtaccgcc tcttggcctg tctggccagg catctcggga
     2101 aggtcgtctc cagctccgaa ctgctcaagg agatgagcgg ctacgactgc tccgagcagg
     2161 aagcccagga gatcgtcaag gtgcacgtga gccggcttcg cagcaagctg gataaggatc
     2221 ccggcaaggc cagctacctg ctcaacgtcc gcggcttcgg ctacttactg gagcggcgca
     2281 tctccgcggc acgctccatg gcatagctgc tgtgctagaa tagggcctgc gttagaggtg
     2341 tcgtctaacc tcgaggtgtc gatgaagccg cccctccctt cggcccgcgt caatctgctc
     2401 gttctcctgc tcgcgctctc cctgcttctg tctgtcaccc atcccccaat ctcacagatc
     2461 cctctcgcct gggcatcccc tgaccagtca gaaacgcccg cagctcaatc cgatcgccag
     2521 gatttcgtgc ctggcgagtt gctggtgaag ctgcgaggag aaagccctga gttgccgctg
     2581 tcccttatcc aatcgctggg ctaccccata tccgtactgg agaaaatgga ggggttgggg
     2641 gtgttgcgcc tcagcgtgcc ggccggcagg gagatggaga gcatacaact g
//
LOCUS       DATALS010000085         4730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_141785, whole genome shotgun sequence.
ACCESSION   DATALS010000085 DATALS010000000
VERSION     DATALS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141785"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /gene="glmS"
                     /locus_tag="VHS06_01850"
     CDS             complement(<1..1279)
                     /gene="glmS"
                     /locus_tag="VHS06_01850"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX2986901.1"
                     /translation="MSIILDGLTRLEYRGYDSAGFAVLSSGEIVVRRSAGKLANLANI
                     AKENPAFGSIGIGHTRWATHGAPNETNAHPHRDCNDEVVIIHNGIVENYTALKKGLLE
                     RGHAFRSQTDSEIIAHLIEEGLQAGAAPVDAIREALTQLRGAHAIVVLTTREPGRIYA
                     ARIGNAGGVCIGQGDGEMFVASDMPAILSHTRRLVFLDSHELAVVQKDGASYFRLDGE
                     LVEKTPQTVNWDPISAAKGGYKHFMLKEIHEQPRSLSDTLSGRLGYQPPAVYLEDFGM
                     TDEELRSRKKVIIVSCGTAWHASLVGKFLIEGLARLPVEVDYASEFRYRDSLVDPTTL
                     VVAVTQSGETVDTLVSMERAKAVGAGLVGIVNVVGSQATRVADGVIYTRSGPEIGVAS
                     TKAFTGQLMALYLLALRLGQAHGTISGEQMAQLI"
     gene            complement(1585..2577)
                     /locus_tag="VHS06_01855"
     CDS             complement(1585..2577)
                     /locus_tag="VHS06_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986902.1"
                     /translation="MFTPRFWLCLVLLVSLAAGLVGCGAAAPTPTSVPTATPVPTATP
                     TPAPTATPTPQPTSTPTPTATPKSLAHVLNVLMARSVDNQQRAVDPITTFGPQEKVFA
                     SAELSNLSGGTVISAKWLKDEELLKTISVTADQDYPSVFVSFSLEPSEPLPDGKYRVE
                     IYVEDQLGITGYYYIDAATETADSDLTAYENTDLGFRVGYPPDWVVSEDSEGVTFQKP
                     GAVVLFFVQVVPGATGNTPKEISDSFVEGFREQAPDLEVQLTQEVEKVGTTWYEADGE
                     YTETNTNTKLTMGSFSTVQGDRAYIIAMLAPSATLQDEIADSFTPMLNSFAFLE"
     gene            2821..3294
                     /locus_tag="VHS06_01860"
     CDS             2821..3294
                     /locus_tag="VHS06_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986903.1"
                     /translation="MRSVSYEETVPAGKVVVGGILAGIILCLGLAITFVVQGLPAPML
                     ILLALVVFLSLLLWNFRAIHLTVDGEAFAADFGVFNRTHIPLGEITSCRPTRATFGRY
                     MGIGVRAGTDGTWAYTTWFGPAVEVRAAGRRPFVVSTKNPAAVCDAIEQARSGRG"
     gene            3350..3426
                     /locus_tag="VHS06_01865"
     tRNA            3350..3426
                     /locus_tag="VHS06_01865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3384..3386,aa:Arg,seq:tct)
     gene            3477..3553
                     /locus_tag="VHS06_01870"
     tRNA            3477..3553
                     /locus_tag="VHS06_01870"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3511..3513,aa:His,seq:gtg)
     gene            3605..3681
                     /locus_tag="VHS06_01875"
     tRNA            3605..3681
                     /locus_tag="VHS06_01875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3639..3641,aa:Arg,seq:tcg)
     gene            complement(3789..4151)
                     /locus_tag="VHS06_01880"
     CDS             complement(3789..4151)
                     /locus_tag="VHS06_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986904.1"
                     /translation="MDEVAAAIRQLYGDAVRVKVLPDKRHPENVFVGMCERKNFSDRA
                     EWLCQKYGYDIDAGWVTIGQMNLPKDSTQVLEIPIGNPIDDQFEAAILSISNGIHALV
                     KPRFPAIAFTPICIYRNC"
     gene            4351..>4730
                     /locus_tag="VHS06_01885"
     CDS             4351..>4730
                     /locus_tag="VHS06_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986905.1"
                     /translation="MHHYVFSQVEEALDSGEKIAQFDSSQDSREWATARFRDGQLIRA
                     VGLVRLMDYSSASGVFEVLPKLQRMLYELAVYDLKRSRDSGRISVHEFEAQRKEKQKE
                     LSAIKDMPVDEVAAAIRQLYGDAVR"
BASE COUNT          837 a         1408 c         1559 g          926 t
ORIGIN      
        1 ctatcagctg cgccatctgt tcacctgaga tggttccatg ggcctggccg aggcggagcg
       61 caagcagata gagggccatc agctgcccgg tgaaggcctt ggtcgaggcc acaccgatct
      121 cgggcccgga gcgcgtgtag ataacgccgt ccgccacacg ggtggcctgg ctgccgacca
      181 cgttgacgat gccgaccagg ccggccccca cggcctttgc ccgctccatg gagacgagag
      241 tgtccactgt ttccccggac tgcgtcaccg ccaccaccag cgtggtggga tctacgagcg
      301 agtcgcgata gcggaactcg gaggcgtagt ccacctccac cggaaggcgg gccaacccct
      361 cgatcaggaa cttgccgacc aggctggcgt gccaggcggt gccgcaggac acgatgatga
      421 ccttcttgcg gctgcgtagc tcctcgtccg tcatgccgaa gtcctccagg tagacggcgg
      481 gaggctggta gccgaggcgc ccgctcaggg tgtccgagag cgagcgtggt tgctcgtgga
      541 tctccttcag catgaagtgc ttatagccgc ccttcgctgc ggagatcggg tcccagttta
      601 ccgtctgggg cgtcttctcc acgagctcgc cgtcgaggcg gaagtagctc gcgccgtcct
      661 tctgtacgac ggccagttcg tgactatcga ggaagaccag gcgccgggta tggctgagga
      721 tggccggcat gtccgaggct acgaacatct cgccatctcc ctgcccgatg cagacgcctc
      781 cggcgttgcc gatgcgggcc gcgtaaatgc gacctggctc gcgcgtggtg agcacgacga
      841 tggcgtgcgc accgcgcagc tgcgtcaatg cctcacggat ggcatcgaca ggcgcggctc
      901 ctgcctgcag tccctcctcg atcaggtgcg cgatgatctc ggagtcggtt tgcgatcgga
      961 aggcgtgccc gcgttcgaga aggcccttct tgagcgccgt gtagttctcc acgatgccgt
     1021 tgtggatgat cacgacctcg tcgttgcagt cccgatgcgg atgggcgttc gtctcgttcg
     1081 gcgctccgtg tgtggcccag cgcgtgtgcc cgatgccgat gctaccaaag gcggggttct
     1141 ccttggcgat gttcgccagg ttggccagtt tccccgcgct gcggcgcacg acgatttcac
     1201 cgctgctgag aaccgcaaag ccggcggagt cgtagccgcg gtactccagg cgcgtgagac
     1261 cgtcgaggat gatggacatg gcctcttgat cgccgacata accgacaatg ccacacatgg
     1321 atccgttcct ccactgaagt gcaagaaccg agacgccctg gccgaagtag agcaagaagc
     1381 acaccaggga ggtcggtccc tgcggcatgg aacgctcagc cgccaactgg gacgctgcat
     1441 tctagcatgc ggcgggcgga ccgttccacg ttgggaggct gggggagcgc agcccgctcg
     1501 ccagaatggc aggctggtcg ctggtccagc ggccagcctg ccgtaggcta cggtctcatg
     1561 ccaggtgcgt gggctggcac tgagtcactc gaggaaggcg aacgagttca gcatcggagt
     1621 gaagctgtcg gcaatctcat cctgtagggt cgctgaaggt gccagcatgg cgatgatgta
     1681 cgccctatcg ccctggacag tagagaagga tcccatcgtc agcttcgtgt tggtgttcgt
     1741 ttccgtgtac tcaccgtctg cctcgtacca ggtggtcccc accttctcca cttcctgcgt
     1801 cagctggacc tccagatccg gcgcttgctc gcggaatccc tcgacgaagg agtcggagat
     1861 ctctttggga gtgtttcccg tggcgccggg gacaacctgc acgaagaaga gaaccactgc
     1921 tccgggtttc tggaacgtca caccctcgga gtcttccgac acgacccagt ccggcgggta
     1981 cccgacgcgg aagcccaggt ccgtgttctc gtaggccgtg aggtccgaat cggcggtctc
     2041 ggtcgccgcg tcgatgtagt agtacccggt tatgccgagc tggtcctcca cgtagatctc
     2101 gacgcggtac tttccatcag gcagcggttc cgacggctcc agggagaagg agacgaagac
     2161 ggaaggatag tcctggtccg cggtgacgct gatggtcttc aacagttcct cgtccttgag
     2221 ccacttggcc gagatcacgg tgccgccgct gaggttggag agctccgccg aggcgaagac
     2281 cttctcttgt gggccgaagg tggtgatggg atcgacggcg cgctgctggt tgtccaccga
     2341 tctggccatt agcacgttga ggacgtgggc gagcgacttc ggtgtagcgg taggcgtggg
     2401 agtgctggtc ggctgcggcg tcggcgtggc ggttggggct ggcgtgggtg tggcggtggg
     2461 aacgggcgtc gcggtgggaa cgctggtagg cgtgggcgct gccgctccgc aaccaaccag
     2521 gccggctgcc agcgacacca gcaaaacgag gcacaaccag aaacgcgggg tgaacattag
     2581 gggccctcct cttcctcacg gcactttcag aaggttcagg cggctgcccg ccagagtgtc
     2641 actcagcata acacggcggc gaagggcagg caatagtgtc aaccagttgt cacggaaacg
     2701 gccagcagtc gtgcccagaa tgccttcctc gacgcagctt caatggtatc ccgtaaccgg
     2761 tgccactcgg ccttcgtagc aatggggtga agagagacgc gcagaggagg atcgggtcgc
     2821 atgcgtagcg tttcgtacga ggagaccgtg ccggcgggca aggttgtcgt gggaggtatc
     2881 ctggcgggga tcatcctctg tcttggactc gccatcacgt ttgtggtgca ggggctaccg
     2941 gctccgatgc tcattctgct cgcgctcgtg gtctttctgt ccctgctact ctggaacttc
     3001 cgcgccatcc atctcacggt ggacggcgag gccttcgcgg cggacttcgg cgtcttcaac
     3061 aggacgcaca ttccgctggg ggagataact tcctgccggc ccacgcgggc gaccttcggc
     3121 cggtacatgg gcatcggcgt gcgcgccggt accgacggga cctgggctta caccacctgg
     3181 tttgggccgg ccgtggaggt gcgagccgca gggcggcgac ccttcgtggt ctccacgaag
     3241 aacccggccg cggtctgcga cgccattgag caggcgcgct cggggcgggg atgacgaggg
     3301 cggttgttga cggtccggcg tgcggtactc tataatatcc aggcagcgtg cgcctgtagc
     3361 tcaggtggat agagcagctg ccttctaagc agcgggtcgg gggttcgagt ccctccaggc
     3421 gtaccagtaa cgctcgatcg ctggccgaga aggagcagga cgggcgtgcc ggcttggtgg
     3481 gcgtagccga gtggtttaag gcgccaggtt gtggccctgg agatcgtggg ttcgaatccc
     3541 atcgctcacc ccagacgggc ctctccgatg cggtgaggcc cgtcgttctg ccaagtgaat
     3601 acctgcgccc gtagctcagt ggattagagc atctgacttc ggatcagagg gtcgggggtt
     3661 cgaatccctc cgggcgtgcc atgggcccca gttgctgcag gtcgctgaca gcacatgtca
     3721 caagggattc gcgtgcctcc cccatttcaa cttgtccaag ctcgtgtgct gccggtgtca
     3781 gactagggtc agcaattgcg gtagatgcag atcggcgtga aagcgattgc cgggaacctc
     3841 ggtttcacca aggcatggat gccgttgctt atgctgagta ttgcggcctc aaactgatca
     3901 tcgattgggt ttccgatcgg tatctccagc acctgtgtcg agtctttcgg cagattcatc
     3961 tgaccaatgg tcacccagcc agcgtcaata tcgtatccgt atttctggca cagccattca
     4021 gctctatcgc tgaagttctt gcgctcacac atcccgacaa acacgttctc agggtgacgt
     4081 ttgtcgggca ggaccttgac ccgcacggca tccccgtaca actgtctaat ggcggcagcc
     4141 acctcgtcca cgggcatgtc cttgatcgcg ctcagctcct tctgctccta cgccgcccag
     4201 atcttgcacg gtctgcccga aggcgctaca acagggacga gccaagaggt tacggacagg
     4261 ggcggaatcg aagtcgggtt ccccagcttc ctcaaagggc agctcggtac agaccaccgg
     4321 ttctttcgca ccgaaaacga gactcgcagc ttgcatcact acgtcttctc tcaggtggag
     4381 gaagccctag acagtggtga gaagatcgcc caattcgatt cgagccagga ctctagagag
     4441 tgggcaacgg cgcgctttcg tgatgggcaa ctgatccgag cggtgggcct cgttcgactg
     4501 atggactaca gttcggcttc aggcgtattt gaggtcctgc cgaagctgca acgcatgctg
     4561 tacgaattgg cggtgtacga cctgaagagg tccagggatt ccgggcgcat cagtgtgcac
     4621 gagtttgaag cgcagcgaaa ggagaagcag aaggagctga gcgcgatcaa ggacatgccc
     4681 gtggacgagg tggctgccgc cattagacag ttgtacgggg atgccgtgcg
//
LOCUS       DATALS010000086         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_141889, whole genome shotgun sequence.
ACCESSION   DATALS010000086 DATALS010000000
VERSION     DATALS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141889"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..453
                     /locus_tag="VHS06_01890"
     CDS             223..453
                     /locus_tag="VHS06_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX2986906.1"
                     /translation="MQRAYVLIETVGGKSKEVADTLRKVPGIVASDVVTGPYDVICVA
                     QGPDANSVGRLVLTEIRGVQGITKTMTCLAVE"
     gene            509..787
                     /locus_tag="VHS06_01895"
     CDS             509..787
                     /locus_tag="VHS06_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986907.1"
                     /translation="MEPWNVKSIEERRGERQSRIRPYTEVQLFFLLRLERLLRLRREH
                     ATLSNSSDWWAKLLSKAIYSTYCDCIEQGVGEDARKLLARDQAAATEA"
     gene            complement(848..>2030)
                     /locus_tag="VHS06_01900"
     CDS             complement(848..>2030)
                     /locus_tag="VHS06_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986908.1"
                     /translation="PSLTPSLSRSLTRFLGVQAMKVTFPHAGQAWVPLSTLFAKAGVE
                     CIVPPPCSKRTLSLGVKHSPEWVCLPFKLNLGNFIEALEMGADTLVYVAGPGLCRLGY
                     YAKVAEQILRDAGYEFEMVCFDWQEKQIVGLAEFLRKLLGDRPWPRIVRDIKFGLDQM
                     AVMDDLERMAHRLRPREAKPGQITAIWRTAGERVSKAWTGADLKRVKSEIIAEYNAVP
                     QNLEVDPLRIGLLGEFFMVTEPFTNMDLEDMLGRMGVEVTRHAFFSEWAKVWLFLEAL
                     GLSHGQKVKRAAAPYLSRDVSGDAVQTVGETILHQEEGYDGIIHLLPFTCMPEIIAQS
                     IMPRVTKDHEIPVLSIVLDEQMGRAGFVTRIEAFVDLMQRRRDLKRRAAQTVAAPNTN
                     S"
BASE COUNT          381 a          645 c          623 g          381 t
ORIGIN      
        1 ttgggaagaa tagcaaacag caaacagaag gatcgcgtgg cctcctgtcc gctccgtcca
       61 ccaggtccac ctcgtccact ccccgctccc ccacgggcat gccgtctgcg gaggttgccc
      121 tccgccacgg ggtattgaca acccatgggg aaggcctaat atggagttgg cgcaaggatc
      181 tggcgcctca ggtaagcacc aggccagtgg aaggtgttat cgatgcagag agcttacgta
      241 ctgatcgaaa ccgtcggcgg gaaaagcaaa gaagtggccg ataccctgcg caaggtacca
      301 ggcatcgtgg cttccgacgt cgtcaccggt ccgtacgacg tgatctgtgt cgcacagggt
      361 cccgatgcca actccgtcgg acggctggtt ttgaccgaaa tccggggtgt tcagggcatc
      421 accaagacca tgacttgcct ggccgtggaa tagcctggcg gtggcaggtc acggggtaaa
      481 gccctgccgc gagagtgagg tgagcggggt ggaaccttgg aacgtcaagt caatcgaaga
      541 gcggaggggc gagcggcagt caagaatccg gccttacact gaggtccagc tcttcttcct
      601 cctcaggctg gagcggcttc tgcggctcag acgggagcac gcgaccctgt cgaactccag
      661 cgactggtgg gcgaagctgc tgagcaaggc gatctactcg acctactgcg actgcatcga
      721 gcagggtgtg ggagaggatg ctcgcaagct gttggccaga gaccaggcgg ccgcgacaga
      781 ggcctgactc gcagcccgtg ctcgggactt gcgcgtccga tgccggacta cggtccgcac
      841 tgccttccta gctgttcgtg ttcggcgcgg ccacggtctg cgccgcgcgt cgtttcagat
      901 ctcggcgcct ctgcatgagg tccacgaagg cctcgatcct cgtcacgaac cccgccctgc
      961 ccatctgctc gtccaggact atcgacagca ccggtatctc gtgatccttc gtgaccctgg
     1021 gcatgatgct ctgggcgatg atctcgggca tgcaggtgaa cggcagcagg tggatgatgc
     1081 cgtcgtagcc ctcctcctgg tgcaggatag tctccccgac cgtctgcaca gcgtccccgc
     1141 tgacgtccct gctcaggtat ggcgcggccg ctcgcttgac cttctgtccg tggctcagcc
     1201 ccagcgcctc gagaaacagc cacaccttcg cccactcgct gaagaaggcg tggcgcgtga
     1261 cctcaacgcc catcctgccc agcatgtcct ccagatccat attggtgaat ggctcggtca
     1321 ccatgaagaa ctcgccaagc aggccgatgc gtagcggatc gacctcaagg ttctggggaa
     1381 ccgcgttgta ctccgcgatg atctcggact tcacgcgctt caggtcggcg ccggtccacg
     1441 ccttcgagac ccgctctcct gccgtgcgcc agatggccgt aatttgcccg ggcttcgcct
     1501 cccttgggcg cagcctgtgc gccatccgct cgaggtcgtc catcacggcc atctggtcga
     1561 gaccgaactt gatgtcccgg actatccgcg gccagggtct gtcccccagc aactttctga
     1621 ggaattcggc cagcccgacg atctgcttct cttgccagtc gaagcacacc atctcgaact
     1681 cgtagccggc gtctcggagt atctgctccg ccaccttcgc gtagtagccc aggcggcaaa
     1741 ggcccggacc agccacgtag acgagcgtgt ccgcgcccat ctccagcgcc tcgatgaagt
     1801 tgccgaggtt tagcttgaag ggcagacaaa cccactctgg agagtgtttc acgccgaggg
     1861 aaagcgtccg cttgctgcac ggtggaggga ctatacactc gaccccagcc ttcgcgaaca
     1921 gtgtcgagag cggtacccat gcctgtcccg cgtggggaaa agtcaccttc atggcctgta
     1981 ctccgagaaa tcgagtgagg gagcgagaga gtgagggagt gagtgaaggc
//
LOCUS       DATALS010000087         4750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_366745, whole genome shotgun sequence.
ACCESSION   DATALS010000087 DATALS010000000
VERSION     DATALS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366745"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VHS06_01905"
     CDS             complement(<1..272)
                     /locus_tag="VHS06_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX2986909.1"
                     /translation="MSERVDIPLATLTEVAISAARAGGSALLEQRRIGYRVQFKGEID
                     LVTDADRRSERTVVSVIQTHFPDHQILAEEGSTGGASSRYRWVIDPL"
     gene            complement(413..1270)
                     /locus_tag="VHS06_01910"
     CDS             complement(413..1270)
                     /locus_tag="VHS06_01910"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX2986910.1"
                     /translation="MLKTGIEDPRAKADRWLHTVNNLPSFPPVVQEVLKFLDDKNSDA
                     KTVAVLISKDAAISAKLLRLVNSAFYGLRGEVTTISHAVALLGLGQLRLLLVGVALLE
                     QGRARNPHLDQARKILWEHSFACSNWAQELAKATRYTPVEEAAVASLLHDVGKIALSA
                     ADPKLLLQSIRQSFAEGVPSFEVEERLLGFNHIETGKMIADRWRLPQPVRTAIALHHH
                     PWSIVPQTGEADPSRLKHLVAIVKVANQASKEGVTNQTLAEPTDSTIAITPEALIQNV
                     YAVNNMLLG"
     gene            complement(1685..2776)
                     /locus_tag="VHS06_01915"
     CDS             complement(1685..2776)
                     /locus_tag="VHS06_01915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2986911.1"
                     /translation="MARSCQLACCGVERNCAAGLALAHGDNPYGVYPLSYSFKLDNVT
                     FYAPNLNPPISLPGLAFLSRFDPLDAFWAWFAISVVLYLVTLALLAFAFSRTLTFVHL
                     FWAVSVWGFWQTLQIGNIYIVAFAIVAAAWLSMDRGHHCAAALFVGTLVAMKPQFGLW
                     ALALFIIGYQRTALVAVAIAGVLTIAPVLPFGPDVYRQWLNALTSYDYLAFTENVSLI
                     GMASRANIPALGFVASVLMVGASLFWLWRFRPDWRQASALALVTVLVASPTAWPANGL
                     CLLPYLLSRRVWTPPMGIAAGIMVIPSWLIWVIASVVGVTKYNFGLAGDLYCLGFLLL
                     STELIRQIRSTAALRTGVPIPPPRTPYAW"
     gene            2707..3180
                     /locus_tag="VHS06_01920"
     CDS             2707..3180
                     /locus_tag="VHS06_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986912.1"
                     /translation="MRVRDRLRNYVQHRNMPVGSFSLSAGIEDLQRGVVKHHLGLHFG
                     RDQLLSYDGWSTVKAEISSLPPKFEVTPLLREMTECLHRVKAAMLQDELPELVQSANY
                     FVGLAGDLRAPDSLPCLMMMNGSAAPTQQEKMSISVRYISWHIVDQVLATSDPGS"
     gene            complement(3374..3673)
                     /locus_tag="VHS06_01925"
     CDS             complement(3374..3673)
                     /locus_tag="VHS06_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986913.1"
                     /translation="MELQDLLVFIAGGAAVVVYYLMENVFALASLAPSAKRWAAYLLT
                     AAIAVAAYLFQVVMLNQPAPVDWRGWVESTSAIAFGVIGLNQIIHGQARLGKTSQ"
     gene            complement(3688..4398)
                     /locus_tag="VHS06_01930"
     CDS             complement(3688..4398)
                     /locus_tag="VHS06_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986914.1"
                     /translation="MIAGYLFNVADKVAGPEVVSDPSEDGASYLMGRALFGGPARVGP
                     SVGWRATAGHSLFNRPRAVATVDSILIVDECDIQATLERATTEAWGPLGWDAPIPDDY
                     DPYARRVWEARYHIAKNWAGRGEPPAYGYGLMYSEVIASDGRVFITKAGGARGVGPVQ
                     RKPDRVRHRGGREQGQPSDRCPESQPSVQAGRPTGGVRPGHNGDVFGHGELTAYGNST
                     ECCGPDLLDLVRSYWAGQ"
     gene            4480..>4750
                     /locus_tag="VHS06_01935"
     CDS             4480..>4750
                     /locus_tag="VHS06_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986915.1"
                     /translation="MSAIRQEWPAAIALVWRISVSILLCVLLVPNIVLAYHMDGDLRD
                     FGSFYASGLALAHGDNPYGVYPLSYSFKLDNVTFYAPNLNPPISLP"
BASE COUNT          993 a         1414 c         1420 g          923 t
ORIGIN      
        1 agtggatcga tgacccagcg atacctgctc gatgctccgc ccgtggagcc ctcctcggca
       61 aggatctggt gatcggggaa atgcgtctgg atgaccgaca ccacagtccg ttctgagcgg
      121 cgatccgcat cggtaaccag atcgatttca cccttgaact gaactcgata gccgattcga
      181 cgctgctcga gtaaagcgct tccgccggcc cgggcagcag agatcgccac ttcggtcaat
      241 gtagctagtg ggatgtcgac acgttcactc ataggcaact ggattatagc actgaggttc
      301 gatttcggac cagttgctcg ccctgggttg ccgctccccg actacgcctc acttcggggc
      361 cgaagtagag ggaatcagca ctcagcactc agcactcggt gggactcgcg catcacccga
      421 gtagcatatt gttgactgcg tagacgttct ggatcagcgc ctcgggggtg atggcgatgg
      481 tggagtcggt cggctcggcc aatgtctggt tcgtcacccc ctccttgctt gcctggttgg
      541 ccaccttgac gatggctacc aggtgtttca gcctggaggg atccgcctcg ccggtttgcg
      601 ggacgatgga ccaggggtga tgatggagcg cgatggcggt gcggacgggc tggggcagcc
      661 gccatcggtc ggcgatcatc ttccctgttt cgatgtggtt gaaacccagt agccgctctt
      721 cgacttcgaa ggaggggacg ccctcggcga agctctgccg gatggattgg agtagcagtt
      781 tcggatcggc cgcggagagg gcgatctttc cgacgtcgtg gagcagactc gctacagcgg
      841 cctcctccac cggcgtgtag cgggtggcct tggcgagttc ctgggcccag ttggagcagg
      901 cgaaagagtg ttcccacagg attttccgcg cctgatcgag atgtgggttg cgggcgcgac
      961 cctgttccag caacgcgacg ccgaccagga ggagtcggag ctggcctagg cccagcaggg
     1021 cgacggcgtg agagatggtg gtcacctcac ctcggaggcc gtagaaggcg gagttcacca
     1081 gtcgcagcag ctttgccgaa atggcagcgt ccttgctgat gaggaccgcg accgtcttgg
     1141 cgtcggagtt cttgtcgtct aggaacttca gcacttcctg cacgacggga gggaaagagg
     1201 gcaggttgtt gacagtgtga agccagcggt cggccttggc gcgaggatcc tcgatacccg
     1261 ttttcagcat tgccggccct ccaatgtgac ggggtaccat agactccaac ggagacacca
     1321 acgcgactgc gccgcgctgc ccaggcgcgg ccctgtcgaa gcatgtcttt gcgggggact
     1381 cgaaatggtg aacgattgcc gccaacgtcc cagagtgcaa caaccgccac tgtagcaggc
     1441 tcattgtagc acgaccaacc catgcgtgtg ggggaaaaag gggaaaagta ggtacagtcc
     1501 tttacatgaa ctttacagat ccagccgtgt catacggcca actggccgct ggggccggta
     1561 agaagaccca gatgttcggt gccagcccgc gccttaaggt agaagaactc gggcggcgac
     1621 gagatcggtg ttgagggatt ctctggggcg ctgattgatt cacggacgcc catagggcac
     1681 tacgtcacca tgcatagggc gttcgtggtg gcgggatcgg cacacctgtt cggagtgccg
     1741 ccgtgctgcg gatctggcgg atgagctctg ttgagagcag gagaaacccc agacaataca
     1801 ggtcaccggc caacccgaag ttgtatttgg tgactcccac gacggatgca attacccata
     1861 tcagccacga ggggatgacc ataatgcctg cagcgatacc catcggaggc gtccatactc
     1921 ttctggagag caggtaggga agcaggcaca gcccgtttgc cggccaagcg gtgggggagg
     1981 caacgagaac agtgaccaga gcgagggcgc tggcttgcct ccagtccggt cgaaaacgcc
     2041 acagccagaa gagggatgct cccaccataa gaaccgatgc tacgaacccg agggctggga
     2101 tattggcgcg cgatgccata ccgatcagag agacgttctc ggtgaacgcc agatagtcgt
     2161 atgaagtgag ggcattcaac cattgccggt acacgtccgg accgaatggc aagacagggg
     2221 cgattgttag aactcctgct atcgcgacgg cgacaagcgc agttcgctgg taaccgatga
     2281 tgaaaagggc cagagcccag agcccaaact ggggtttcat ggctacgagc gttccgacga
     2341 atagggctgc ggcgcaatga tgccccctat ccattgacag ccacgcagca gcgacgattg
     2401 cgaacgctac gatatagatg ttgcctatct gaagggtctg ccagaaaccc caaacactga
     2461 ctgcccagaa cagatggacg aaggtcaatg tgcgactgaa ggcaaatgcc agaagcgcca
     2521 gcgtcaccaa gtacaggacg accgagatgg cgaaccacgc ccagaacgcg tctaacggat
     2581 caaagcgcga taggaatgcc agacccggca gggatatggg agggttaagg ttaggagcgt
     2641 agaaagtgac gttatccagc ttgaacgaat agctgagtgg atagacacca taggggttgt
     2701 ctccgtgtgc gagtgcgaga ccggctgcgc aattacgttc aacaccgcaa catgccagtt
     2761 ggcagcttct cgctatcggc tgggatcgag gacttgcaga gaggggttgt gaaacaccac
     2821 cttggcctcc attttggccg tgaccagctt ctatcctacg atggctggtc aactgtgaag
     2881 gccgagattt cctcgctccc gcccaagttc gaggtcacac cgctcctacg ggagatgaca
     2941 gagtgtttgc acagggtgaa ggccgcaatg ctacaggacg aactgcctga actggtgcaa
     3001 agcgccaact acttcgtggg cctcgccggt gacctgagag cacctgatag cctcccctgc
     3061 ctaatgatga tgaatggttc agcagcgcca actcaacagg agaagatgag catctccgtc
     3121 aggtacattt cttggcacat cgtcgatcag gtgctcgcaa ccagtgatcc aggttcctag
     3181 cctgcgacag cttacctacg cacgatattg agtatccaag tctgcggcat caggggccat
     3241 ctaccttatc gcaacaggct tccactgtcc cctttccagc cgccatctcc cgtaggattg
     3301 caggaaaagg acaggcccgg agcgtggtgg ggcgctccgg gcctatgtcg cggcaggagg
     3361 atgctgccgc cgtttactgg ctggtcttgc cgagccgggc ctgtccatgg atgatctggt
     3421 ttaggccaat cacgccgaag gcaatggcgg aggtggactc aacccagcca cgccaatcca
     3481 ctggggcggg ctggtttagc atcaccacct ggaacagata ggccgcaacg gcgatcgcgg
     3541 cggtgagcag gtaggccgcc caccgcttcg ccgacggggc caggctggcg agggcaaaca
     3601 cgttctccat caggtagtag accaccactg cggcgccgcc agctataaac accaacaggt
     3661 cttggagttc catcgtctcg ctccttccta ctggccagcc cagtaggacc gcaccaagtc
     3721 caacagatct ggtccacaac actccgtcga gttgccgtag gctgtcagct cgccgtgccc
     3781 aaacacgtcc ccattgtgcc caggccgaac tccgcccgtc gggcgtccag cctgaaccga
     3841 aggctgcgat tctgggcatc ggtcggaggg ctgaccttgc tcgcgtccac ctcgatggcg
     3901 tacccgatcc ggtttgcgct gcactgggcc cacgccgcgt gctccaccgg ccttagtgat
     3961 gaacaccctg ccatcgctgg caatcacctc gctgtacatc aacccgtatc cgtaggccgg
     4021 cggctctcct ctgcccgccc agttcttggc tatgtggtag cgagcctccc agacgcgccg
     4081 ggcatacgga tcgtagtcat cggggatcgg ggcgtcccat cccaatggac cccatgcctc
     4141 agttgtcgcc cgctccagcg ttgcctgtat gtcacactcg tccacgatca gaatgctgtc
     4201 gaccgtagct accgcgcggg gtcggttaaa gagagaatgc cccgcggtcg ccctccaccc
     4261 gacagatggc ccaaccctcg ccgggccgcc gaagagagcg cgtcccatca ggtaagaagc
     4321 gccatcctcg gacgggtcac tcaccacttc cggccccgcc accttgtcgg caacgttaaa
     4381 gagataccct gcaatcatcc tcttctttgt acacgcatcg cgctcagtct gtggtattat
     4441 ccactacatt gttgcaccat caactatgtg gagatggccg tgtctgccat tcgacaggaa
     4501 tggcccgccg cgatcgcatt agtctggagg atatctgtct caatactgct atgcgtgctg
     4561 ctcgtcccga acatcgttct cgcatatcac atggacggcg acctgcgaga ctttggctcc
     4621 ttctacgcat ccggtctcgc actcgcacac ggagacaacc cctatggtgt ctatccactc
     4681 agctattcgt tcaagctgga taacgtcact ttctacgctc ctaaccttaa ccctcccata
     4741 tccctgccgg
//
LOCUS       DATALS010000088         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_142384, whole genome shotgun sequence.
ACCESSION   DATALS010000088 DATALS010000000
VERSION     DATALS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142384"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VHS06_01940"
     CDS             complement(<1..1187)
                     /locus_tag="VHS06_01940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2986916.1"
                     /translation="MNDYGSHAKTSLEAPLAGDADRVGVLPERAPSVEPQGPQRERLR
                     HWLNWHDRRWQILSCTLLATTFAISAATIRGYGITWDEEHHKEYGVILLNWYMSLGHD
                     TEALSKWNLYYYGGFADTLAQLFAQHVPFGVYEARHIVCLLFGLVGIAATYGLGHRIA
                     GPRGGFFSALFLTLTPVYYGHMFNNPKDIPFAAMFTLALLTMLLAFDSLPRVPKRRIL
                     LTGIVTGLAMGIRVNGVVLLALQALLWGGWLLGRCWCNPFSISRSIGHLAIDLGKRFV
                     VVVLVAWSVMLVTWPWALVSPILHPLEALLRTTDYDWPLTVFFDGRFIKASELPSSYL
                     PTWFAISMPEFYFAALQVGIILTAAFLRGFTPTMEHINRLLKTGLLVGVFLVPILAAT
                     ILKA"
     gene            1499..>1708
                     /locus_tag="VHS06_01945"
     CDS             1499..>1708
                     /locus_tag="VHS06_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2986917.1"
                     /translation="MSLSDPSSRQRAYDAIKEAILSNQFPPGESLAEQMLADLYQVSR
                     TPVREALQRLAKDGLVEMIPRRGAFV"
BASE COUNT          360 a          478 c          540 g          330 t
ORIGIN      
        1 gccttcaaga tggtcgccgc cagaatcgga accaggaaga ctcctaccag caggccggtc
       61 ttcagaagcc ggttgatgtg ctccatggtg ggtgtgaagc cccgaaggaa agctgctgtc
      121 aggattatgc cgacctggag cgccgcaaag tagaactccg gcatggagat cgcgaaccag
      181 gtcggtaggt agctggaggg gagttcgctg gccttgatga accgcccgtc gaagaatacc
      241 gtcagtggcc agtcgtagtc ggtggttctc aggagagcct cgagggggtg gaggatcgga
      301 gagacgagcg cccacggcca ggtgaccagc attacgctcc aggccaccag gaccacaacg
      361 acgaaccgtt tcccgaggtc tatggccaag tgaccaatgc ttcgactgat tgagaatggg
      421 ttgcaccagc atcgcccgag gagccagcct ccccatagca gagcttgaag ggccagcaac
      481 acgaccccgt ttacccggat ccccatggcc aatcccgtaa cgatgcctgt gaggaggatc
      541 cgtctcttcg gcactcgcgg tagggagtcg aaggcgagca gcatggtgag gagggcgagc
      601 gtgaacatcg ctgcgaaggg gatgtccttc ggattgttga acatgtgccc gtagtagacg
      661 ggcgtgagtg tcagaaacag ggctgagaag aagcctcccc tgggccccgc gattcgatgc
      721 cccagaccat acgtggcggc gatgcccact agaccgaata ggaggcacac gatgtggcga
      781 gcctcgtaca ccccgaacgg gacgtgttgg gcgaatagtt gtgccagggt gtccgcaaat
      841 ccaccgtaat agtacaggtt ccacttgctc agggcctcgg tgtcgtggcc cagggacatg
      901 taccagttta gcaggatgac cccatactct ttgtggtgct cctcgtccca ggtgatgccg
      961 tagcccctta tcgttgcggc cgagatggca aatgtcgttg ccagcagcgt gcacgataag
     1021 atttgccacc ttctatcgtg ccaattcagc caatggcgaa gacgctcacg ttgggggcct
     1081 tggggctcga cggatggggc cctctcgggc aagactccaa cacgatcagc gtctcccgct
     1141 agcggagcct cgaggctggt cttcgcgtga cttccgtaat cgttcaagaa ccacctcaac
     1201 tgaaggacgg aggggcgata ctactctcta tgcattgcgc aaacacagag taccttgttg
     1261 tagaacgaat cggcaggaag tgtgctaagt aactgctaaa agtgtctagc agttctgtca
     1321 tcgaggagga ctccggtgat gatctgtgtt gtctgctcat tggctggctg cgcagtgtcg
     1381 aggacattcg tcagcgcggc agatcggcgg ccgggagggt attgcattat ccggcgcgaa
     1441 gcgtatactg gcggcatcct gactgtatac agattgtatc caagaggagg ccggcgccat
     1501 gtcgttgtcc gatccctcga gccgccagcg ggcttacgac gcaatcaagg aggcgatcct
     1561 ctccaaccag tttccccccg gagagtcgct cgccgagcag atgctggccg atctctacca
     1621 ggtcagtcgt acccccgttc gcgaggcgct gcagcggctt gcgaaggacg gcctggtgga
     1681 gatgatcccc aggcgggggg ccttcgtc
//
LOCUS       DATALS010000089         6047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_48769,
            whole genome shotgun sequence.
ACCESSION   DATALS010000089 DATALS010000000
VERSION     DATALS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48769"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..735)
                     /gene="coaE"
                     /locus_tag="VHS06_01950"
     CDS             complement(1..735)
                     /gene="coaE"
                     /locus_tag="VHS06_01950"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX2986918.1"
                     /translation="MSMVLGVTGGIATGKSTVLAMLKELGAAVLDADSVARDVLAPGS
                     EGESEAISEFGAEILGADGRIDRRKLGERVFGDENALKRLNEITHPRIIAILDQRIGE
                     FRKDHADDGVLAVEIPLLVECDLSYLVDKVIVVVSEQAAQIHRLKMRSKLSAEEAAQR
                     IAAQIPVSEKEEYGDWVVRTDGTLEETRRQVEQIWRQIGTKPQQSLYQPDTTDRTPPA
                     EVSPPPAPRHQRGLSGTPLSADRVWL"
     gene            complement(823..2412)
                     /gene="rpsA"
                     /locus_tag="VHS06_01955"
     CDS             complement(823..2412)
                     /gene="rpsA"
                     /locus_tag="VHS06_01955"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX2986919.1"
                     /translation="MSDEKNEVTNAADVEMAGTPLAEEQAQQASEPQKEEHGPSVDYS
                     RTFRELTEGDVVGGVVVHIDKEGVLVDVGTKSEGIIRLHELARDSSLTPEEVVAVGDH
                     IDVYVMETESHDGNLILSKKRADFEKAWERVEQAMQEGKTLTAMVSDRVKGGLVVDLG
                     IRGFVPASHVGSGKVKNLDKYVGQSIPLRVIEVDRERRKVVLSNRVAVDDEREKQKAE
                     TLATLAEGQIRDGIVRRVTDYGAFIDLGGIDGLLHISEMSWTRIQHPSEVVKVGQKLQ
                     VMVLKLNLDQNKVSLGLRQILPDPWADVSDRYHVSDVITAPISRLVPFGAFVQLEGGI
                     EGIIPNSELAYKRVSKPEDIVQVGQTVEIKILDLRPEERRMTLSLKALQQAPPREQRE
                     PREPQYTYTQPEHRTTIADLVGDVMNQRKFKRLKAREAQRAEEEAAAEDLASMEAEET
                     EPMFPDEPVAPEAEAETTAEVEAEATPEVEAEAAPEVEAEAAPEAEAAEEAPAAEAEA
                     VEVQAPEVEQEKAPETEEKAE"
     gene            complement(2919..5657)
                     /gene="polA"
                     /locus_tag="VHS06_01960"
     CDS             complement(2919..5657)
                     /gene="polA"
                     /locus_tag="VHS06_01960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX2986920.1"
                     /translation="MVTELKDTKHTHKLVLVDGNSLLYRAFFAMRYLSTSEGQPTNAV
                     YGFTMMLLHLIEEEKPDSIVVAFDAPAKTFRHEQFEGYKAQRKPAPDELKSQAPIARE
                     LIRAFNIPMVEIEGFEADDVVGTLARMAEEKGYTTLIVTGDLDALQLVSDKVHVLTTI
                     KGVTDTVEYDAKAVEERFGLRPDQMVDYKALKGDPSDNIPGVSGIGDKTASKLIKLYG
                     TLEEVLKNIDQIKESRVQNALVGATEQAEQSKMLATIVRDVPLDINIDECKYEGPDYE
                     RLRDLFRGLEFKTLLKRLPEVEGEEQQAAAPMEAAVLGCSVVVESPADLDRLITHLRG
                     ANSVAVRVHTEGEKAAEAKLLGIGFSTGVDESYYVRLAEKQAEQGTLDFGSDGSFVAD
                     LSQFKDILESETVRKFGHNLKHDFGVMKQHGIALKGISFDTMLGAYVLNSSRGSYKLP
                     DVALEQLGLELPQPEKPAKKSEGEGTDPRTVVCGEVEAVHRLVPVLIDKLERDGLMLL
                     LQDVEMPLVEVLAEMELRGVSIDTGYLAALSAALDIRIREIQQQIYDEAGMEFNIGSP
                     KQLQEVLFEKMNLPVGKKTKTGFSTGADTLEQLAPMYPIVEQILQYRELAKLKSTYAD
                     ALPKLINARTGRLHTSLNQAVTATGRLSSSEPNLQNIPVRTEIGREIRKAFVAGNDNL
                     LVSADYSQIELRILAHVTGDKALVEAFENDRDIHLATACTIFAVPPEEVTPEMRRRAK
                     TINFAVIYGMSDFGLARELGLTNKLAREYIDNYFARIPGVRKYTEETLDEAREKGYVQ
                     TLLGRRRYIPEIHSGNRNYRMFAERAAVNMPIQGTAADIMKIAMINVHNKLESMDCAS
                     QMVLQVHDELVFEVPPSEADHIVPIVRDLMETAYPLIVPLKTDVKLGKNWAEMESV"
     gene            complement(5658..>6047)
                     /locus_tag="VHS06_01965"
     CDS             complement(5658..>6047)
                     /locus_tag="VHS06_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX2986921.1"
                     /translation="PMRPDERALIKSMIMRTYSQFVSDVSKGRKMPASKVRSLADGRV
                     YTGTEAKKLGLVDEIGGLRETVLAAGRAGRIKGEPKVKEYGQGGLAGALFGSEDEEMS
                     GRPLGDAVMLRFVRSLLGSEQSYQGLR"
BASE COUNT         1112 a         2073 c         1570 g         1292 t
ORIGIN      
        1 tcatagccat actcgatcag cggatagggg agttccggaa agaccacgct gatgacgggg
       61 tgctggcggt ggagatacct ctgctggtgg agtgcgatct gtcgtatctg gttgataaag
      121 tgattgttgt ggtttcgttc caatctgccg ccagatctgc tctacctgac gacgggtctc
      181 ctccaacgta ccgtctgttc ggacgaccca gtcgccatac tcttccttct cggagaccgg
      241 tatctgcgca gcaatcctct gcgccgcttc ttcagctgac agcttgcttc tcattttcag
      301 acgatgaatc tgcgcagctt gttccgaaac cacaacaatc actttatcaa ccagatacga
      361 cagatcgcac tccaccagca gaggtatctc caccgccagc accccgtcat cagcgtggtc
      421 tttccggaac tcccctatcc gctgatcgag tatggctatg atccgcgggt gggtgatctc
      481 attgaggcgc ttgagggcgt tctcatcgcc gaaaacccgt tcgccgagct tccttcggtc
      541 tatccgccca tctgctccga ggatctccgc cccaaactcg ctgatagcct ccgattcgcc
      601 ctccgaaccg ggggccagaa cgtccctggc cacgctatcc gcatccagaa ccgcagctcc
      661 aagctccttc agcatcgcca gaacggtgct tttcccggtg gcaatccccc cggtcacccc
      721 aaggaccatc gacatcaacc tctacctcca cacaaaaata aagcgtggat gaaatcattc
      781 atccacgctc taatgtgcct atcgatcact caacaggacg agttattcgg ccttttcctc
      841 ggtctcagga gccttctcct gttcaacttc gggggcctga acttctacag cctcagcctc
      901 agcagccggc gcttcctcag cggcctcggc ctcaggggcc gcctcagcct ctacctcagg
      961 agcagcctca gcctctacct caggggtggc ctcagcctct acctcagcag tcgtctcggc
     1021 ctcagcctca ggagcgaccg gctcgtccgg gaacatcggc tcggtctcct cagcctccat
     1081 cgaagccaga tcctcagccg ctgcctcttc ctcggccctc tgagcctctc gcgccttgag
     1141 ccgcttgaac ttgcgctggt tcataacgtc accaacgaga tcagcgatcg tcgtgcgatg
     1201 ctcgggctgg gtataggtgt actgcggctc acgcggctcg cgctgctcgc gcggcggcgc
     1261 ctgctgcagg gccttcagac tcagagtcat acgcctctct tcaggcctca agtcaaggat
     1321 cttgatctcg acggtctgcc cgacctggac tatgtcctca ggcttggata cgcgcttgta
     1381 agccagttcc gagttcggga tgataccctc gataccgccc tcgagctgga cgaatgcacc
     1441 gaacggaacc agcctggaga tgggagcggt gatgacgtcg ctaacgtggt agcggtcgct
     1501 gacatcagcc cacggatccg gcagtatctg cctcagacca agagaaactt tgttctggtc
     1561 gaggttcagc ttcaggacca taacctgcag cttctgcccg actttcacga cctcagacgg
     1621 atgctggatt cttgtccacg acatctcgct gatgtgcagc agtccatcga taccaccaag
     1681 gtcgatgaac gcgccgtagt cggtcactcg gcggacgata ccgtcgcgaa tctggccttc
     1741 cgcaagagta gccagcgtct cggccttctg cttctcacgc tcgtcgtcta cggcaactct
     1801 gttggacaga acgaccttgc ggcgctcgcg gtcgacttcg atcactctca gcggaatcga
     1861 ctggccgaca tacttgtcga ggttcttgac cttaccgctg ccaacgtgcg atgccgggac
     1921 gaaaccgcgg ataccaaggt cgacaacaag tccgcccttg acccggtcgc taaccatagc
     1981 ggtcagcgtc ttgccttcct gcatcgcctg ctccacgcgc tcccaagcct tctcaaaatc
     2041 ggcgcgcttc ttggaaagaa tcaggttgcc gtcatggctc tcggtctcca taacgtagac
     2101 gtcgatgtgg tctccaaccg caacgacctc ctcaggggtc aggctgctgt ctctggcaag
     2161 ctcgtgaagc ctgataatac cctcagactt cgtgcctacg tccacaagaa cgccctcttt
     2221 gtctatatgc accaccacgc cgcctacgac gtctccctct gtcaactcgc ggaacgtgcg
     2281 cgagtagtca actgaaggtc cgtgctcttc cttctgtggc tcggatgcct gctgggcctg
     2341 ctcctccgca agcggagtgc cggccatctc aacatcggca gcgttagtga cttcgttctt
     2401 ctcgtcagac atttacggaa tctcctctcg ttggcgcctc taccgggcat acaccggtat
     2461 gtctcttgga gtcgccagtt tagtcgtctc ggcggtggtc agcgttcatc accgatcaaa
     2521 tgagtctttg ccagcagaca agccacctaa ggacaggccc gccggcacgg cctgagtggt
     2581 ataacgccga cgctgctgac taccaccagg ccgtcagcac gtacagcact caaggcgcgt
     2641 tcgctgccgc gccatgatca tcccggaaat cggcgaatat acattagaca cccacgcgcg
     2701 atttcctcct acatatgata gtctgaagct tgtccagagt caagcataat caggcctcta
     2761 tagccacacg tgctcccctc cccgcgtcgg ggaggggctg gggggagagg tcagcagtca
     2821 ggaaccagac ccattgaggc cgccgcacat gcgcccgctg cacaacttca ccgctcacaa
     2881 cctatacacc accgagcctg cttgctaact ttgagcccct acaccgactc catctcggcc
     2941 cagtttttgc ccaacttcac gtccgtcttc agcgggacaa tcagcgggta ggccgtctcc
     3001 atcagatcgc gcactatcgg aacgatatgg tccgcctcac tcggcggcac ctcgaagacc
     3061 aactcgtcgt gtacctgcag caccatctgg ctcgcgcagt ccatcgactc cagcttgtta
     3121 tgcacgttga tcatcgcgat cttcataatg tcggccgcag taccctgtat cggcatgttc
     3181 acggccgccc gctcggcgaa catccggtag ttgcggttgc ctgagtggat ctccggtata
     3241 taccgccgcc ggccaaggag ggtctgcacg tagcccttct cccgggcctc atcgagcgtc
     3301 tcctcagtgt acttgcggac cccggggatc ctggcgaagt agttgtcgat atactccctg
     3361 gccagcttat tcgtcaaccc aagctcgcgc gccagtccaa agtcgctcat cccgtagatc
     3421 acggcgaagt tgatcgtctt cgcccgacgc cgcatctccg gagtcacctc ctccggcggc
     3481 acggcgaata tcgtgcaggc cgtagcgagg tggatgtccc tatcgttctc gaacgcctcc
     3541 accagcgcct tgtcgccggt cacgtgagca aggatcctca gctcgatctg cgagtagtcc
     3601 gccgacacca gcagattgtc gttgccggcg acgaacgcct tccgtatctc cctgccgatc
     3661 tccgttctca ccgggatgtt ctgcaggttc ggctcgctcg acgacagccg gcccgtggcc
     3721 gtaaccgcct ggttcagcga tgtgtgcagc cgtcccgtgc gcgcgttgat cagcttaggc
     3781 agcgcatcgg catatgtcga cttcagcttc gcaagctccc gatactgcag tatctgctcc
     3841 actatcgggt acatcggcgc caactgctcc agagtgtcag ccccggtcga gaaccccgtc
     3901 ttggtcttct tccccaccgg caggttcatc ttctcgaaga ggacctcctg aagctgcttc
     3961 ggcgagccga tgttgaactc catccctgct tcatcgtata tctgctgctg aatctcacgt
     4021 atgcggatgt ccaacgccgc cgacagagcc gccaggtaac cggtgtctat gctcacaccg
     4081 cgaagctcca tctcggcaag cacctcgacc agcggcatct cgacatcctg aagcagaagc
     4141 atcagcccgt cgcgctccag cttgtcgatc agcaccggga ccaggcggtg taccgcctcg
     4201 acctccccgc acacaaccgt ccggggatcg gtcccctccc cctcagactt tttggccggc
     4261 ttctccggct gagggagttc cagccccaac tgctccaagg ccacatcggg aagcttatag
     4321 ctgccgcgcg aggagttcag cacataggct ccgagcatag tgtcgaagct gatccccttc
     4381 aaggcgatcc cgtgctgctt cataaccccg aagtcgtgct tcaggttgtg gccgaacttc
     4441 ctgaccgtct ccgactccag gatatccttg aactgcgaga gatcggcgac aaacgaccca
     4501 tccgacccga agtcgagcgt cccctgctcg gcctgctttt cggcaagccg gacgtagtag
     4561 ctctcatcga ctccggtgga gaacccgatc cccagaagct tcgcctcggc cgccttctca
     4621 ccctcggtgt ggacgcggac agcgacactg ttcgcccccc gcaggtgagt gatcagccgg
     4681 tccaggtcgg caggcgactc cacaaccacg gagcagccca acaccgccgc ctccatcgga
     4741 gccgcagcct gctgctcctc accttcgacc tccggcagcc gcttcagcag agtcttgaac
     4801 tcaaggccgc ggaacaggtc ccgcagccgc tcgtagtccg gcccctcgta cttgcactcg
     4861 tcgatgttga tgtccagagg gacgtcgcga acgatcgtcg caagcatctt gctctgctcc
     4921 gcctgctcgg tcgccccgac cagggcattc tgtaccctcg actctttgat ctggtcgatg
     4981 ttcttgagga cctcttccag ggtgccgtac agcttgatca gcttggatgc ggtcttgtcc
     5041 cctatcccgg acacgccggg gatgttgtcc gacggatcgc ccttcagcgc cttgtagtcc
     5101 accatctggt cgggccgaag tccgaaccgc tcctccacag ccttcgcgtc gtactccacg
     5161 gtatccgtca cgcccttgat cgtggtcagc acgtgcacct tgtcgctcac caactgcaga
     5221 gcgtccaggt cgccggtgac gatcagggtg gtgtagccct tctcctcggc catcctggcc
     5281 agggtcccca cgacgtcatc ggcctcgaac ccctcgatct cgaccatggg gatgttgaac
     5341 gcccggatca gctcgcgggc gatgggggcc tgcgacttca gctcgtcggg cgccggcttg
     5401 cgctgggcct tgtagccctc gaactgctcg tggcggaacg tcttggccgg agcatcgaac
     5461 gccacaacta tcgaatccgg cttctcctcc tcgatcagat gcaggagcat catcgtaaag
     5521 ccgtagaccg cgttcgtcgg ctgcccctcc gaggtggaga ggtaccgcat cgcaaagaac
     5581 gcgcggtaaa gcaggctgtt cccgtccacc aggaccagct tgtgggtatg tttcgtatcc
     5641 ttcagttcag ttaccatcta tctcaaacct tgatatgact gctcggaacc gagcaaagat
     5701 ctgacaaacc ggagcataac ggcatccccc aaagggcggc ccgacatctc ctcatcttcg
     5761 gatccgaaca gggccccggc aagtccgccc tggccgtatt ccttcacctt cggctcgccc
     5821 ttgatacgtc cggcccggcc cgcggccagc accgtctccc tcagcccgcc gatctcatcg
     5881 accagcccca gcttcttcgc ctccgtgccg gtatagaccc gtccgtcggc gaggcttctg
     5941 accttcgacg ccggcatctt ccgccccttg ctcacatctg agacgaactg ggagtaggtg
     6001 cgcatgatca tgctcttaat cagcgccctc tcatccggac gcatcgg
//
LOCUS       DATALS010000090         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_409666, whole genome shotgun sequence.
ACCESSION   DATALS010000090 DATALS010000000
VERSION     DATALS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409666"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..295
                     /locus_tag="VHS06_01970"
     CDS             71..295
                     /locus_tag="VHS06_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX2986922.1"
                     /translation="MGHVQIDEELCKGCELCGEACPRHLMRLSARINRQGYHPAEFVA
                     PEESNGKSKGCTGCALCGLVCPETAISVYR"
     gene            312..1406
                     /gene="vorB"
                     /locus_tag="VHS06_01975"
     CDS             312..1406
                     /gene="vorB"
                     /locus_tag="VHS06_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HEX2986923.1"
                     /translation="MAGIVNDPAAFDGRVLMEGNEAFGEGAIRAGCDAYFGYPITPQT
                     ELLEFMSRRMPALGRAFVQAESEVAAINMVLGAAASGARGMTSSSSPGISLKQEGISY
                     LAGDELPAVIVNVMRGGPGLGNVAPSQGDYFQATKGGGHGVYHPIVLAPASVQEAMDL
                     TALAFDLADRYRTPVMILADGVIGQTVEPVVLKEYGRRDDLPPKDWALVGCAGRERRI
                     IKSIEMEPEVMERVDQRLVAKYDLIKQREVRWTGSYLDDARLAVVAFGTPARVSLSAA
                     RAARAAGIPVGLFRPITLFPFPEREIAELAGRVDAILVVELNQGQMVEDVERVSAGRT
                     LVRHFGRLGGVVVSPEEVLDALKSLWEETR"
     gene            1403..>1955
                     /locus_tag="VHS06_01980"
     CDS             1403..>1955
                     /locus_tag="VHS06_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2986924.1"
                     /translation="MITSEEQLVFNRPRSLLNGLTHYCPGCGHGIVHRLVAEAIDELG
                     LRERTVAVYPVGCAVFAYNYFDVDGCEAAHGRAPAVATGIKRTHPNLFVFTYQGDGDL
                     GAIGTAEVVHAAARGENISVVFVNNAVYGMTAGQMAPTTLVGQHTTSTSSGRDPARSG
                     YPIRVSEMLATLEGPSYITRVSVH"
BASE COUNT          350 a          558 c          693 g          354 t
ORIGIN      
        1 cgaaagcggt cggcggccgg ccagcgatcc tgggaggacg ccgacagtcc atcgattaag
       61 ggggtcagag ttggggcacg tacagatcga tgaggagctg tgcaaggggt gtgagctgtg
      121 tggggaggcg tgtcctcgcc atcttatgcg gctctcggca cgcatcaacc gccagggtta
      181 ccaccctgcg gagttcgtcg ccccggagga gtcgaacggc aagagcaaag ggtgcaccgg
      241 ttgcgctctg tgcggtctcg tctgccctga gacggcgatc tccgtctacc gctgagtgga
      301 ggaaatgacc gatggctggg attgtcaacg acccggccgc gttcgatggc cgggtgctga
      361 tggagggaaa cgaggcgttc ggagagggcg ctatacgagc cggctgcgat gcatacttcg
      421 gttatccgat cactccacaa accgagttgc tggagttcat gtcgaggaga atgcctgccc
      481 tggggagagc attcgttcag gccgagagcg aagtggcggc gatcaacatg gtgttgggtg
      541 ctgctgcatc cggggccaga ggcatgacgt cgtcgtccag cccgggcatc agcttgaagc
      601 aggaggggat ctcctacttg gcaggcgacg agcttcccgc ggtgatcgtc aacgtgatgc
      661 gaggcggccc tgggctcgga aacgtggctc catcgcaggg ggattacttc caggccacca
      721 aggggggcgg gcacggcgtc taccatccta tcgtgctggc acctgcgtcg gtgcaggagg
      781 cgatggatct cacggcgctg gccttcgacc tggccgatcg gtaccgaacg cccgtaatga
      841 tccttgcgga tggagtcatc ggtcagacgg tggagcccgt ggtgctgaag gaatacgggc
      901 gacgcgatga ccttccgccg aaggattggg cactggtggg ctgtgccggc cgcgagagac
      961 ggatcatcaa gtccatagag atggagccgg aggtgatgga gcgcgtcgac cagcgtctgg
     1021 tggcgaagta cgacctcatc aagcagcggg aggttcgctg gaccggcagc tacctggacg
     1081 atgccagact ggcagtggtc gccttcggca cccccgcacg tgtctctctc agcgcggccc
     1141 gcgccgcccg ggctgccggg atccccgtgg gcctcttcag gcccattacc ctcttcccat
     1201 tccccgagcg agagatcgcc gagcttgccg ggcgcgtcga cgcgatcctg gttgtcgagt
     1261 tgaaccaggg ccagatggtg gaggacgtgg agcgggtgtc cgcggggcgc acgctggtgc
     1321 gccatttcgg acggctgggt ggagtcgtcg tatcgcccga ggaggtgctg gacgcactca
     1381 agtctctctg ggaggaaaca cgatgattac cagcgaggag caactggtct tcaaccggcc
     1441 gcgctcactc ttgaatgggc ttacccacta ctgccccgga tgcggccacg gcatcgtcca
     1501 cagactggtc gccgaggcga tcgacgaact ggggctgagg gagcgcaccg tcgccgtcta
     1561 ccctgtcggt tgtgcggtat tcgcttacaa ctacttcgac gtagacgggt gcgaggcggc
     1621 tcatggccgg gcacccgcgg tggcgacggg gatcaagcgt acgcacccca acctgttcgt
     1681 atttacctac cagggggatg gtgacctggg cgctatcggc acggcggagg tcgttcatgc
     1741 ggcggcgagg ggcgagaaca tcagcgtcgt cttcgtcaac aatgcggtgt atgggatgac
     1801 tgccggccag atggctccca cgaccctggt tggtcagcat actacctcga cgtcatcggg
     1861 aagggatccg gcaaggtcgg ggtatccgat ccgcgttagc gaaatgctgg cgacgctgga
     1921 gggcccgagc tacatcacga gggtatctgt gcaca
//
LOCUS       DATALS010000091         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_8272,
            whole genome shotgun sequence.
ACCESSION   DATALS010000091 DATALS010000000
VERSION     DATALS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8272"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VHS06_01985"
     CDS             <1..543
                     /locus_tag="VHS06_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2986925.1"
                     /translation="WWIRQAITRAIADQARTIRIPVHMVETINKLSRLSRRLLQDLGR
                     EPTSEEIGSKMEIPAEKVREILKVSQEPVSLETPIGEEEDSHLGDFIEDQSALAPAEA
                     ASHQLLKEQVDDVLRSLTHRERRVLQLRFGLDDGRQRTLEEVGREFGVTRERIRQIEA
                     KALRKLRHPSRSKKLKDYLD"
     gene            complement(673..>1830)
                     /locus_tag="VHS06_01990"
     CDS             complement(673..>1830)
                     /locus_tag="VHS06_01990"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX2986926.1"
                     /translation="VNAMARAIVLAIASLRRTETARRAAQQLTLSGLRFQAILEALPQ
                     GVLVVDSPDGRVATANRSFQRLTGRYIAPGSPADSYCGMLSAARRVGSGIPDEIPWIR
                     CAATGEASDSEEMVVSRPDGQIMTVLCSTSPILDEAGKVVGAVALLQDISARKELELQ
                     KDEFLAMVAHELKTPLTSVKGYVQLLVRQAQKTPNATFGEKEIGMLQIADRQVTRLGQ
                     LVFDLLDCSSIQLGRLVLRLTSFDLGLLAGEVVAQMQAASPERNIAFLNCGETVVRAD
                     PQRMEQVLINLIANAVKATEHGGRIDVTVRRDGHRVVVSVRDDGMGIAMDLQQRVFER
                     LFRGPAHKYEGMGLGLYICKGIMDAHGGSIWLESEEGKGSTFYFAIPVLQP"
BASE COUNT          346 a          560 c          573 g          351 t
ORIGIN      
        1 tggtggatac gccaggcgat cactcgtgct atcgccgacc aggcacggac catccgcatc
       61 ccggttcaca tggttgagac gatcaacaag ctcagccggc tatcgcggcg gcttcttcag
      121 gatttggggc gtgagcccac ctctgaagag attggcagca agatggagat cccggcggag
      181 aaggtgcgcg agatcctgaa ggtttcccag gaacccgtct ctctcgagac cccgatcgga
      241 gaggaagagg atagccacct gggtgacttc atcgaggacc agtctgcact ggctcccgcc
      301 gaagccgcgt cccaccagtt gctgaaggag caggtggacg acgtgcttcg ctcgctgact
      361 cacagggaga gacgggtgct gcagctcagg tttggcctcg atgacggccg gcagcggacg
      421 ctggaagagg tcggacggga attcggcgtt accagggagc gcattcggca aatcgaggcg
      481 aaggccttgc ggaaattacg ccatcccagc cgcagcaaga agctgaagga ctacctggac
      541 taatcctctg agagggtagt gtcgacatca atggggcgga tcgcatgcga tccgccccat
      601 tgtctgtgag agcgccggca tgcgctcgca cgcttcgtct tctccggaag tagccatttc
      661 cggcacccca ctctaaggct gcaggactgg gatcgcgaaa tagaaggtgg aacctttgcc
      721 ctcctcgctc tccagccaga tgctgccgcc atgcgcgtcc atgatcccct tgcagatgta
      781 caggcccagc cccatgccct cgtacttgtg cgcggggccg cggaaaagcc gctcaaacac
      841 ccgttgctga aggtccattg cgatccccat gccgtcatcg cggacggaaa cgacgactcg
      901 gtgtccgtct cgccggacgg tgacgtcgat ccttcccccg tgttcggtgg ccttgacggc
      961 gttggctatc aggttgatca gcacctgctc cattcgctgc gggtcggccc ggactactgt
     1021 ctcgccacag ttgagaaaag cgatgttgcg ctcgggcgag gccgcctgca tctgcgcgac
     1081 gacctcccct gccagcagcc ccaggtcgaa ggaggtcaat cggaggacga ggcgtcccaa
     1141 ctgtatggag gagcagtcga gcaggtcgaa gaccagttgc cccaggcggg tgacctgtcg
     1201 gtcggcaatc tggagcatgc cgatctcctt ctccccgaag gtggcattgg gggttttctg
     1261 ggcctggcgc acgaggagct gcacgtagcc tttgaccgat gtgagcggtg tcttcagttc
     1321 gtgggcaacc attgcgagga actcgtcctt ctgcagctcg agttcctttc gagccgatat
     1381 gtcttgcaga agagcgactg cccctactac cttgcctgct tcgtcgagta tcggggatgt
     1441 gctgcacaag acagtcatga tctggccatc cggacggctg accaccatct cttcggaatc
     1501 cgacgcctct ccggtcgccg cgcaccgtat ccaaggtatc tcatccggta tgccgctacc
     1561 tacccttctt gcagcgctca gcatgccgca gtaactgtcc gcgggactgc ccggggcgat
     1621 gtagcgtcca gtgagccgct ggaaggagcg attggcagta gcgaccctgc cgtccgggga
     1681 gtctacgacg aggacaccct gcggaagcgc ctcgaggatt gcctggaacc tgagtcccga
     1741 cagggtgagt tgctgtgccg ctcggcgagc ggtctcggtg cggcgaaggg aagcaatagc
     1801 caatactatg gcccgcgcca tggcgttcac
//
LOCUS       DATALS010000092         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_188337, whole genome shotgun sequence.
ACCESSION   DATALS010000092 DATALS010000000
VERSION     DATALS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188337"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..552)
                     /locus_tag="VHS06_01995"
     CDS             complement(64..552)
                     /locus_tag="VHS06_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986927.1"
                     /translation="MNIMRYTVIDDRGTVSFVADCDALLPMVAGCARDPKSVEELLAI
                     VDEYDSGIEEKVTCSLAIFDEMNTADNHESIHKALDFCRPHELPAFRVLDDRTREASL
                     TPVHAGVVVFNLPARRIVQIQNSYEEIQRRARRKAAKPATPERGVVKYELPDYWSLVP
                     EI"
     gene            complement(565..1740)
                     /locus_tag="VHS06_02000"
     CDS             complement(565..1740)
                     /locus_tag="VHS06_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX2986928.1"
                     /translation="MANGRLAFPAGILFGAAAGALYVVAGRKWLDTKYEMVDWDRVQE
                     VAIRTSGRAPAEGPWTSRRLRSSYEEMVRRLEGPIAEYTGSSLPSADTDVTVMGRKEW
                     IEANVTAFRTLFEPVEEMYREVGRQNTVIVPGLKRLSQMAVSSQMGLLLGYLSRRVLG
                     QYDMSLLGREPMSTGKLYFVQQNIEGIESELGIPPEEFRAWITLHESTHAHEFEAFPW
                     LRYYMNHTLRAYLDCVLQEVRKAPTHTTGAAAALVARMAENMRSGRSLLESVMSRRQQ
                     RYMESLQALMCLLEGYSNHVMNEVGKLHMPHFEEIKARVDSRSGDRAQTERLFLKLTG
                     LSMKMEQYKLGEQFVDKVVKERGIQFVNKAYQGPENLPTMDEIRHPRKWIARMEGDD"
     gene            complement(1757..>2402)
                     /locus_tag="VHS06_02005"
     CDS             complement(1757..>2402)
                     /locus_tag="VHS06_02005"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEX2986929.1"
                     /translation="GMVPSGTGSDLARSLGMARGIDEACARLAAPERMITDLGMVTYR
                     VGGEEDRRYFVNAAGIGYDAEVVSRRNGFNRYMRGTLPYLASVATTLMTYHNKDVTIA
                     MDGSAARHRVTAVVVAIGRYFGGGMRVAPNALLDDGLFDVITVGDVGHMELVYNFPRV
                     YRGTHLTHPKIGAERARLVTVESDHPVLVQADGELLGMAPVTFQILPRALTLLR"
BASE COUNT          436 a          765 c          681 g          520 t
ORIGIN      
        1 gcacctcacc aagcaggacg ggacaggagc gacgcgccct acttggtgag gtgcccctgt
       61 cgactagatc tccggtacca gcgaccagta gtccggcagt tcgtacttga ctacccctcg
      121 ttccggcgta gccggtttgg ccgccttgcg ccgagcgcgt ctctggatct cctcgtagct
      181 gttctggatc tgcacgatcc gcctggccgg caggttgaat accacaactc cggcgtgcac
      241 tggagtcagg ctcgcctccc tcgttcgatc atccagcact cgaaaggctg gaagttcatg
      301 cgggcggcag aaatccagtg ccttgtggat cgattcgtgg ttatcggcag tgttcatctc
      361 gtcgaagatg gcgaggctgc aggtaacctt ctcttcgatc ccgctgtcgt actcgtccac
      421 gatggccagc aactcctcga cgctcttggg gtcacgggcg caacccgcga ccatcggcag
      481 cagggcgtcg caatcggcga cgaagctgac cgtaccacga tcatctataa cggtgtaccg
      541 catgatgttc acggttttga ccttctagtc atccccctcc atcctcgcta tccatttgcg
      601 ggggtgccgg atctcatcca tcgtcggcag gttttcgggt ccctggtacg ccttgttgac
      661 gaactgtatt ccgcgttcct tcacaacctt gtcgacgaat tgttcgccca acttgtactg
      721 ctccatcttc atggagaggc ccgtgagctt taggaagagt cgctcggtct gagcgcgatc
      781 cccggaccgg gagtcaaccc gagccttgat ttcctcgaag tggggcatgt gaagcttacc
      841 cacttcattc atcacgtgat tgctgtaccc ctccagcaaa cacatcagcg cctggagcga
      901 ctccatgtag cgctgttgtc gcctactcat gacagattct agcagacttc gtcccgacct
      961 catgttctcc gccatgcgtg ccactagggc tgcggctgcg ccggtggtgt gggttggggc
     1021 tttgcgaact tcttgcagaa cgcagtccag gtaggcgcgg agggtgtgat tcatgtagta
     1081 ccgaagccag gggaacgcct cgaactcgtg ggcgtgcgtc gactcgtgaa gggtgatcca
     1141 tgcacggaac tcctccgggg gaatccccag ttccgactcg atcccctcaa tgttctgctg
     1201 aacgaagtag agcttgccgg tggacatggg ctcccgacca aggaggctca tatcgtactg
     1261 gccaagaacg cggcgcgaga ggtagcccag cagtaacccc atctgactgc tcacggccat
     1321 ctggctcagt cgcttcaggc cgggcacaat cacagtgttc tgtcgtccga cctccctgta
     1381 catctcctcc acgggctcga agagcgtgcg gaaggctgtg acgtttgcct cgatccactc
     1441 ctttcggccc atcacagtga cgtctgtgtc tgccgagggg aggctgctcc cagtgtactc
     1501 ggcgatcgga ccttccagcc gcctgaccat ctcctcgtat gaggagcgca atcgccggga
     1561 agtccagggc ccctctgcag gtgcacggcc ggaggttcgg atggctacct cctgcacgcg
     1621 atcccagtcc accatctcgt acttcgtgtc aagccacttg cgccctgcaa ccacgtagag
     1681 ggcgcctgca gcagcaccga agagtattcc cgccggaaag gcgagtctgc cgtttgccat
     1741 tgtttccctt tctagtctaa cgaagaaggg tgagggcgcg ggggaggatc tggaacgtga
     1801 caggagccat gcccagcagt tcgccatcgg cctggaccag gaccggatgg tcagattcca
     1861 ccgtgaccag tctggccctt tcggcgccga tcttggggtg tgtcagatgg gtcccccgat
     1921 agaccctagg gaaattataa accagttcca tatgtcccac atccccgacg gtgatcacat
     1981 cgaacaagcc gtcgtccagc agggcattcg gcgcgactcg catccctcca ccgaagtacc
     2041 ggcctatggc taccacgacg gccgttaccc gatgtctcgc tgcactcccg tccatggcaa
     2101 tcgtcacgtc tttattgtgg taggtcatca gcgtcgtcgc gacgctggcc aggtagggga
     2161 gtgtgccgcg catgtagcga ttgaagccgt tgcggcggct cacaacctcc gcgtcgtagc
     2221 cgatgcccgc ggcattcaca aagtagcgtc ggtcttcttc tccgcccacc ctgtaggtga
     2281 ccatccccag gtccgtaatc attcgctcgg gagcagcaag ccttgcgcat gcctcgtcga
     2341 tccccctggc cattcccagc gatctggcca ggtcggaacc ggttccactg ggaaccatgc
     2401 ct
//
LOCUS       DATALS010000093         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_367056, whole genome shotgun sequence.
ACCESSION   DATALS010000093 DATALS010000000
VERSION     DATALS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367056"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VHS06_02010"
     CDS             <1..616
                     /locus_tag="VHS06_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HEX2986930.1"
                     /translation="AENAEAAIAAGADVGVRVLPGIEVETREGVHVLTLFDRIEKLIQ
                     WQEVVYDHLPPRENDERVFGAQLVVDREGNLLGVNRRLLLTAVDLSLEEVVERSQGYG
                     GLCVPAHVDRPANGLLSVLGMLPEGLRAPALEISPRIDIDGALKQYPQLAGNTLIRSS
                     DAHTLEDIGKVATTLYMERVSLDEFVLACQGDLGRGVAGAHPVC"
     gene            670..1164
                     /gene="nuoE"
                     /locus_tag="VHS06_02015"
     CDS             670..1164
                     /gene="nuoE"
                     /locus_tag="VHS06_02015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX2986931.1"
                     /translation="MPVSQTPMIDLSEVDAILAQARLQHSTIIPVLQDTQEVYGYLPR
                     EALSRIAQVMEVPFSQVYGVATFYSQFYLNRRGKNIVRVCDGTACHVRGAARTLETLR
                     SDLKIEPGDTTEDYQFTYEIVYCLGACALAPVAVVNGEVKRKCSSERMHKLVTDLKST
                     IAAQ"
     gene            1301..1882
                     /locus_tag="VHS06_02020"
     CDS             1301..1882
                     /locus_tag="VHS06_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2986932.1"
                     /translation="MRELSQHLMDLIENSIEAGSRKVAISIDEDLSRDLLTIRVEDDG
                     HGMSQDAVTRATDPFFTSRRCRKVGLGLPLVAATAKRCGGFLTIDSTPGRGTTVTVVL
                     CHSHIDRPPIGDLHSTLVTAFAAHTGVDIVYRHRVGQKCFELDAAAIRRVLEGVPITH
                     PAVLQWLDRFVSDGLAEVGACAPVEEKDDAKAE"
     gene            1866..>2144
                     /locus_tag="VHS06_02025"
     CDS             1866..>2144
                     /locus_tag="VHS06_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX2986933.1"
                     /translation="MPKLNSLEELRRLKEQAQQEIKVRSDTGTRITVGMGTCGISAGA
                     KEVMHAILHELQRREIEANVATVGCAGLCSMEPLVDIEQGGAPRVTYGK"
BASE COUNT          442 a          616 c          711 g          375 t
ORIGIN      
        1 cgcggagaac gcggaggccg cgatagctgc aggggcggac gtcggggtga gagtccttcc
       61 cggcatcgag gtcgagacca gggagggggt ccacgtcctc accctgtttg accggatcga
      121 gaagctgatt cagtggcaag aggtggtgta cgaccacctt ccgcctaggg agaacgacga
      181 gcgcgtgttt ggtgcgcaac tcgtcgtgga tcgggaaggg aacctgctgg gtgtgaaccg
      241 aaggctgctg ttgacggcag tcgacctttc gctggaggag gtggtggagc gatcccaagg
      301 gtacggtggg ctgtgtgtgc cggctcatgt tgaccggccc gccaacgggc ttctttccgt
      361 gctgggtatg cttccggagg gtctccgggc gcccgcgctg gagatatcgc ctcgtatcga
      421 catagatggg gcgttgaagc agtatccaca acttgccggc aacacactga tcaggtcgtc
      481 cgatgcccat acgctggaag acatcggcaa ggttgccacg accctctaca tggagagggt
      541 ttcgctggat gagttcgtcc tcgcctgcca gggcgacctg gggagagggg tggccggtgc
      601 ccaccccgtc tgctgaggag tagtcaccct tcggtcgccg ccaatagcct acgaagggag
      661 gccgggtccg tgccagtgtc acagacgccg atgatagacc tctcggaggt cgacgcgatc
      721 ctggcgcaag caagacttca gcacagcacc atcattccgg tcctgcagga cacgcaggaa
      781 gtctacggat acctcccccg cgaggcgctg tcgcgcatcg cacaagtcat ggaagtcccc
      841 ttcagtcaag tgtatggcgt cgcaaccttc tactcccagt tctacctgaa ccgcagggga
      901 aagaacatcg tccgcgtgtg cgatggaacg gcctgccacg tgaggggtgc ggcccgcacg
      961 ctggagacgc tgcgtagcga tctgaagatc gagcctggag ataccacaga ggactaccag
     1021 ttcacctacg agatagtcta ctgcctgggc gcatgcgccc tggccccagt cgccgtcgtc
     1081 aacggagaag tgaagcgcaa gtgcagttct gaacggatgc acaagctcgt gaccgacctg
     1141 aagtcgacta tcgccgctca gtaggaagcg gggtgcaggg cacaaccctg gcgagcgtcc
     1201 acaagagcgg gtccagggcg cagcgcctgg cggggagtgg ggtgtcccca caagactaat
     1261 accctcattc gcttttgtcg accgaaggac ggcgaaaggg atgcgagagt tatctcagca
     1321 cctgatggat ctgatagaga actcgatcga ggcgggcagc cggaaggtgg ccatcagcat
     1381 cgacgaggac ctatcgcgcg acctcctcac tatcagggtc gaggatgatg gccacggcat
     1441 gtcacaggac gcggtgacga gggccaccga ccccttcttt acctcccgcc gctgccgaaa
     1501 ggtcggcctt ggtcttccac tagtggcggc cacagccaaa cggtgtggcg gcttcctgac
     1561 gatcgatagc accccgggaa ggggcacgac ggttacagtg gttctatgtc acagccacat
     1621 cgataggccc ccgatcggag acctgcactc gacgctcgtg accgcattcg ccgcccacac
     1681 aggggtagat atcgtctatc ggcaccgggt agggcagaaa tgcttcgaac tggatgcagc
     1741 agccattcga cgagtgctgg agggtgttcc cattactcac ccggcggtac tccagtggct
     1801 agaccgtttc gtttccgacg gacttgctga agtgggcgct tgcgcgcccg tcgaggagaa
     1861 ggacgatgcc aaagctgaat agccttgagg agctgaggcg gctgaaagaa caggcacagc
     1921 aggagataaa ggtgcggagc gataccggca ccaggatcac cgtgggcatg ggcacctgcg
     1981 gcatttccgc gggtgccaag gaagtgatgc acgccatcct acatgagcta cagcgacgag
     2041 aaatcgaggc caacgtcgct acggtcggtt gcgcggggct ctgctccatg gaaccgttgg
     2101 tggacatcga gcagggcggg gcaccgaggg tgacctacgg caag
//
LOCUS       DATALS010000094         3672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_48954,
            whole genome shotgun sequence.
ACCESSION   DATALS010000094 DATALS010000000
VERSION     DATALS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48954"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..724)
                     /locus_tag="VHS06_02030"
     CDS             complement(227..724)
                     /locus_tag="VHS06_02030"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX2986934.1"
                     /translation="MPEFLYFAYGSNLDPKLMKNHCPQSREDSVGTLRGYRLGFTFYS
                     SGWGGGTADIVPDSQGEVWGLIYALSPDDLDFLDSYEGHPTKYRRLQVAVDTSDGLVA
                     GVWTYAVVDKEAFIQPTASYLAILTHAAEHYRFPTWYRSMLHSTVTDIRSTDITSAFR
                     NRRPE"
     gene            complement(727..1311)
                     /locus_tag="VHS06_02035"
     CDS             complement(727..1311)
                     /locus_tag="VHS06_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986935.1"
                     /translation="MDEVSASAIGYRDLIVFQLNNADHWLSRGQSSDDSFAQFFFYFA
                     GFNALYFLWKIVDNLDARREGRRTPEIEQIDNLLSKLNDDEAARILSGASRSVAFFSK
                     RPIQQMENRTPLVPGQGEQRKGIERCQQLGDTTATPVDRVTALGRILYLVRCNLVHGG
                     KADRGDDEHVIEKAVPALKALLTASVELTRRKDR"
     gene            complement(1475..2287)
                     /gene="drmC"
                     /locus_tag="VHS06_02040"
     CDS             complement(1475..2287)
                     /gene="drmC"
                     /locus_tag="VHS06_02040"
                     /inference="COORDINATES: protein motif:HMM:NF038319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system phospholipase D-like protein DrmC"
                     /protein_id="HEX2986936.1"
                     /translation="MDNRFAAFLDGIRLLASDLPTSTAEDLAQALESAPGGKWSAILG
                     RCQAVVAHPHYRALVSGMVAAWRLDAPDVEPRSVGLALRLATRTEEKVLEGQSAELVW
                     TGPDPEATPLRRTEQALLQVIGHARHELTVVTFVVYRIPLVAAALVEAAERDVRLRLI
                     AESPEASLGKVDYDAMQALGPAVAGRCQVYIWPRSKRPTTPDGHFGSLHAKCALADDT
                     ELFISSANLTEYALALNMEMGILVQGGPLPGRVKRHIDRLTHTGVLVAIDQG"
     gene            complement(2294..>3672)
                     /locus_tag="VHS06_02045"
     CDS             complement(2294..>3672)
                     /locus_tag="VHS06_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HEX2986937.1"
                     /translation="DFPWITFVHRGPTDCRARLLLRELGVSGEAVDVEVFCEECGARR
                     RMSDAFGPQAKDHMPQCRGRRPHLRDYEEDGCTEKMQTILLGASNSWFSLVQSALSIP
                     APGNRLAQLVTEHWPILEKAANLQNIGLLRQIGQLHAFAQYSDDEVWSAVEAKRSTTP
                     TGQESHDLKSPEWRVLSNPDQANNTSDFYLREVPAPEEFKSVLEKIVLVERLREVRAL
                     VGFTRIESPGDFDEMIELPSEQRAPLSRRPPRWIPATEIRGEGIFVQFKEKQIHDWIR
                     HTKGLDERERDFQEAHRRWRRARGLEVNAGGFPGLRYVLLHSFSHALMRQLALEAGYS
                     MASLRERIYSAPPEAENGPMAGVLLYTAAPDSEGTLGGLVRLGEPLELGRHIGFALEE
                     MRLCASDPLCAEHHPFRDGLTLHGAACHACLFAPETSCERGNKYLDRWLLVDTIASNG
                     VAFFHTEG"
BASE COUNT          722 a         1162 c         1056 g          732 t
ORIGIN      
        1 gatccgtgct atcaattagc cgagacctgg tgatagatct cggggcatga atggcaaacc
       61 gcgctgggga tcaacccagc gcgccggtca cgtaccatat ggttgccaga ccctagcaca
      121 gagacaacca tatggtagcc gagaccgctg tccttgttgc cctcctgggg ctgattggct
      181 ggattccgat gccgccctcg tcccgtcgtc aaggtcgacc cacgacctac tctggacgcc
      241 tgttcctgaa ggcactggtg atatctgtgc tcctgatgtc cgtgaccgtg ctgtggagca
      301 tggatcgata ccaggtggga aagcggtagt gctccgcggc gtgcgtcaag atcgcgagat
      361 acgatgctgt cggttgtatg aacgcctctt tgtctaccac ggcgtaggtc cacactccag
      421 cgactaaacc atccgaggtg tcgacagcca cctggaggcg gcgatacttc gtcgggtggc
      481 cctcgtagga gtcgaggaag tcgagatcgt cgggcgagag tgcgtaaatg agaccccaga
      541 cctccccttg gctgtccggg acgatgtcag ccgtaccgcc accccagcca gacgagtaga
      601 aggtgaaccc caaacggtaa cccctgagtg tgcccaccga gtcctctcga gactgaggac
      661 aatgattctt catcagcttc gggtccagat tcgatccata ggcaaagtag aggaattccg
      721 gcactgctac ctgtcctttc gtcgcgtcaa ctccacgctg gcagtgagca gggccttgag
      781 agcagggacc gccttttcga tgacatgctc gtcatccccc cgatcggcct tgccaccatg
      841 tacaaggttg caccgaacga ggtacagaat cctaccaagt gcggtcacac ggtcgaccgg
      901 agtcgctgtc gtatccccta gttgttggca ccgctcgatc ccctttcttt gttccccctg
      961 gcctggaacc agtggcgttc ggttctccat ctgctgaatt gggcgcttgc tgaagaacgc
     1021 caccgacctg gaagccccgc tcaggatacg tgcggcttcg tcatcgttca acttgctcag
     1081 caagttgtcg atctgctcga tctctggtgt gcgcctgcct tctctgcgcg catcgaggtt
     1141 atcaacaatc ttccacagga agtacagggc attgaatccg gcgaaataga agaagaactg
     1201 cgcgaacgaa tcgtcgctcg actggccacg actaagccaa tggtccgcgt tgttgagttg
     1261 aaacacgatg aggtccctgt atccgattgc gctggcacta acctcgtcca cggcacctcc
     1321 tcctgtccta ccgcttccac tgggggtcaa gcttctaccg acggtgaggc ccccgggatg
     1381 ctcgctatct tcgttgctgg gtttactcag cagagtggcc gtcctgccgc aaacgctcgc
     1441 gtgtcaccac ccgcccgtta tgaccaggcc ccaattaccc ttggtctata gctaccagga
     1501 cgccagtgtg tgtgagtcta tcgatgtgac gcttaacacg cccgggcagc gggccgccct
     1561 gaaccagaat ccccatctcc atgttcagag cgagggcgta ttcggtgagg tttgcgctgg
     1621 atatgaaaag ctccgtgtca tcggcgaggg cgcacttggc atgcagggag ccgaagtgcc
     1681 catcaggggt ggtgggacgc ttggaccgag gccatatgta gacctgacac cgcccggcaa
     1741 cagcggggcc aagagcctgc atggcgtcgt agtcgacctt ccccagactc gcttccggtg
     1801 attcagcaat cagtcgaagg cgaacgtctc gttctgctgc ctcaaccaga gccgcggcca
     1861 ccagtgggat ccgataaacc acgaaggtca cgaccgtcag ttcgtgtcgg gcgtgaccga
     1921 ttacctgcag gagcgcctgc tccgtgcgcc gaagcggggt ggcctctgga tctggtccgg
     1981 tccagaccag ctctgccgac tgtccctcga gcaccttctc ctcggttctc gtagcaagac
     2041 ggagtgctag acctacgcta cgcggttcga cgtccggcgc gtccagccgc cacgcggcga
     2101 ccatcccact gacaagggca cggtagtggg gatgggccac gacagcttgg caacggccca
     2161 ggatcgcgct ccactttccc ccaggggcgg actcaagggc ttgagccaga tcctccgccg
     2221 tagaggtagg cagatcggac gccagaagcc ggatcccgtc caggaacgcc gcgaaccggt
     2281 tgtccatgca ctatcaccct tccgtgtgaa aaaacgccac tccattgctg gctatggtgt
     2341 ccaccagcag ccaacggtcg aggtatttgt tgcccctctc acaagaggtc tcgggagcga
     2401 aaaggcatgc gtggcaagca gcgccgtgca gggtgaggcc atccctgaat gggtggtgct
     2461 cggcacacaa cgggtccgag gcacaaagcc gcatctcctc aagtgcaaac cctatgtgcc
     2521 tccccaactc taggggttcc ccgaggcgaa cgagcccgcc cagggttccc tcactgtcgg
     2581 gagccgcggt gtaaagcaat acgccagcca tcgggccatt ttccgcttcc ggcggggccg
     2641 agtagatgcg ctctcgaagg ctggccatgg agtagccggc ctccaacgcc aactgccgca
     2701 tcaaggcatg agagaaggag tggaggagca cgtatctcaa acccgggaag ccgcccgcgt
     2761 taacctccag cccccgtgct cggcgccatc tccgatgagc ctcctggaag tcccgctccc
     2821 gctcgtcgag ccccttcgta tgccttatcc aatcgtgtat ttgcttctct ttgaattgaa
     2881 cgaagatacc ctctcccctg atctcggtgg ctggaatcca gcgtggtggc cggcgagaga
     2941 gcggtgcgcg ctgctccgac ggaagctcga tcatttcatc gaagtcccct ggcgactcaa
     3001 tgcgggtgaa tcccaccaga gccctcacct cgcgcagccg ctcgaccagg acgatcttct
     3061 cgaggacgct cttgaactcc tcgggcgctg gcacctctcg aaggtagaaa tcggaggtgt
     3121 tgttcgcctg atctgggttc gacagcaccc tccattccgg cgacttaaga tcatgggatt
     3181 cctgacccgt aggcgttgtc gaccgtttcg cctccaccgc tgaccacacc tcatcgtccg
     3241 aatactgggc gaaggcatgt agttgcccaa tctggcgcag caacccgatg ttctgaaggt
     3301 tggctgcctt ctcgagtatc ggccagtgct ccgtgaccaa ctgagccagc ctgtttccag
     3361 gcgcggggat cgatagcgcc gattgaacaa gagagaacca gctattggaa gcgcccagca
     3421 ggatcgtctg catcttctcc gtgcaaccat cctcctcgta gtcgcgcagg tgaggacgtc
     3481 gaccccggca ctggggcatg tgatccttag cctgaggccc aaaggcatcg gacatcctgc
     3541 ggcgtgcgcc gcattcctcg cagaatactt caacgtcaac tgcctcgccg gagaccccca
     3601 gctcccgcag aagcaacctg gccctgcagt ccgtgggacc acggtggacg aaggtgatcc
     3661 acgggaaatc gt
//
LOCUS       DATALS010000095         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_409954, whole genome shotgun sequence.
ACCESSION   DATALS010000095 DATALS010000000
VERSION     DATALS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409954"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VHS06_02050"
     CDS             <1..1096
                     /locus_tag="VHS06_02050"
                     /inference="COORDINATES: protein motif:HMM:NF017013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HEX2986938.1"
                     /translation="RILEVARSANQEDLVFTIFSDGSSSLFPLPRPEYGLEELRDLYK
                     LAIKHGNQEIIVWVMPFFSQVNAGRIVAEAHPARTINLITCTVTYQRWHGKLHTGSCF
                     VSSWPPGAKTMRQWATELQQQPWWPEVPTRMRAALERYDPACELPSLAGFREMRWSYW
                     QPVDAEQMLLAARQKAEELGVHGAILGRWSLANCLEVAHFLSGMARQIALTGQPFEPP
                     VALISTGELTVPVGAATGIGGRNQEFVLGTALRLGRDENSSLYNGLPFGEKMVVAAVD
                     SDGTDGPGTQFSDDAPTDFRCMAGGVVDGQTLARAREIGIDIPAELRNHNSSMPLWQL
                     SDSIYTGNTGTCVGDLRVLLVPERYRGEGI"
     gene            1414..>1985
                     /locus_tag="VHS06_02055"
     CDS             1414..>1985
                     /locus_tag="VHS06_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX2986939.1"
                     /translation="MSKPLKRLTLVVFIVLVCSLSFLAGFGAGQRPLFASVASAQTSQ
                     STDLTEKFRLFWEAWSWVDREFYDRAALDTTKITYGAIRGMFETLGDRHSYFINPQER
                     EVSETQLRGGFQGIGANVELTADGKVRIVAPQEGSPAEEAGIRAGDIILQVDGRSIEG
                     MNLIQAVALIRGPKGTKVRLTLQRDGEAEP"
BASE COUNT          379 a          561 c          657 g          388 t
ORIGIN      
        1 gaggatcctg gaggtcgccc gttccgccaa ccaggaggat ctggtcttca cgatcttctc
       61 cgacgggtcc tcctcactat tccccctacc ccggccagag tacggcctgg aagagctgcg
      121 ggatctgtac aagctggcca tcaagcacgg gaaccaagag atcatagtat gggtgatgcc
      181 gttcttctcc caggtcaatg ctgggagaat cgtggcggag gcccaccctg ctcgcaccat
      241 caacctgatc acctgcactg tcacctacca gaggtggcat gggaagcttc acaccggtag
      301 ctgcttcgtg tcctcctggc cgccgggggc caagaccatg agacagtggg ccacggagtt
      361 acagcaacag ccatggtggc ccgaagtccc gactcgcatg agggcagcac tggagcggta
      421 cgatcctgct tgtgagctac cgagcttggc cggttttcgc gagatgcggt ggagctactg
      481 gcagcccgtg gatgccgagc agatgctgct ggcggctagg cagaaggcgg aggagcttgg
      541 ggtgcacggc gcgatactgg gccggtggtc gctggccaac tgcttggagg tagcgcactt
      601 tctctctgga atggccaggc agatcgcgtt gaccggccaa ccgttcgagc cgcccgtagc
      661 gttgatctcc accggggagt tgacggttcc tgtgggggcc gcaactggga tcggggggag
      721 gaaccaggag tttgtgctcg gtacagcact tcgtctgggg agggacgaga actcgtcgct
      781 gtacaacggc ctgccgtttg gggagaagat ggtcgtcgcc gcggtggact ccgacggaac
      841 cgatggaccc ggcactcagt tcagtgacga tgcgcccacg gacttccggt gcatggccgg
      901 tggcgtcgtg gatgggcaga cgttggctcg tgctcgggaa atcggcatag acataccggc
      961 ggagttgagg aaccacaact ccagcatgcc gctgtggcag ctcagtgaca gcatatacac
     1021 gggaaacacc gggacctgtg ttggagacct gcgagttctt ctagttcccg aacgctatcg
     1081 gggcgagggg atctgaggcc ggtcaagcag tcgccgtcgc tgcacgatgc ccaagctcgt
     1141 cgatccgtcc ctcacggtgc tttgggccga cgcggcccct cccctgcttc attgcgggtt
     1201 gatcttgttg ctgctacact gggatgatgg cgcgcgatca acgttgccga tgaatgtagg
     1261 gcggggtctt gtgccccgcc cacagcactg tctgagccac catagtggtc gtggccacgt
     1321 ttaggaggcg gggcgcaaga ccccgcccta cgaaacgtgt taaggtggac acaagcagca
     1381 gccgcagtcc atcgcgtagc atcagaggga tagttgtcta agcctctcaa gcgtctcact
     1441 ctagttgtct tcatagtcct ggtgtgcagc ctctccttcc tggcgggatt cggtgcgggc
     1501 cagcgtcccc tttttgcctc cgttgccagt gcccagacgt cgcaatccac agatctcact
     1561 gagaagttca ggctgttctg ggaagcatgg agttgggttg acagggagtt ttacgaccgc
     1621 gctgcgctgg acacgacgaa gattacctac ggagccattc gcggcatgtt cgagaccctg
     1681 ggtgatcgtc atagctattt catcaatccc caggagcgcg aggtcagcga aacacagctc
     1741 cgaggcgggt tccagggtat cggggctaat gtcgaattga ccgcggatgg caaggtcagg
     1801 attgtggcac cgcaggaggg ctcccctgcc gaggaggccg ggatacgggc gggggacatc
     1861 atcttgcagg tggacggaag atccatcgag gggatgaacc ttatccaggc agtcgccctt
     1921 atccgagggc ccaaaggcac gaaggtgaga ttgacgctgc agagggacgg agaggccgag
     1981 ccgtt
//
LOCUS       DATALS010000096         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_409983, whole genome shotgun sequence.
ACCESSION   DATALS010000096 DATALS010000000
VERSION     DATALS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409983"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VHS06_02060"
     CDS             <1..348
                     /locus_tag="VHS06_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMO1 family protein"
                     /protein_id="HEX2986940.1"
                     /translation="SEIARLDRTMVIVAADLAHMGPSFGDPEPMDATAKVSLAATDAE
                     MMATICRGDAEGFLAMLTAEQDERKVCGLPPIYLAMKMLGDGITGEVVDYSVCPAPDE
                     SVVSVTGILLLTA"
     gene            complement(345..1145)
                     /locus_tag="VHS06_02065"
     CDS             complement(345..1145)
                     /locus_tag="VHS06_02065"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HEX2986941.1"
                     /translation="MLPREHGAWAMWLVPYLLGSALGGWGIASLMLLISMLFLFISSR
                     PAEFVLTGPTKSSEAQDRRTAAYGRLTIYAGIGVVAAAVLLLRYHRWALLPLGAVAAL
                     ALSIMLPLKARRLDRSWPARLLSIAALSAMGPAAYYVGSGTLDHRAFAVWLLGFLYSG
                     ASVFYVRLFYRPPARKRPATATDPRRSAERDLFVYLAVVLALLIASATVGWAPPFAGL
                     ALAPFLAKAVWAILHRGYQPTLKQLGMGEIGHSAVFALTAFAVMILWV"
     gene            complement(1456..2682)
                     /locus_tag="VHS06_02070"
     CDS             complement(1456..2682)
                     /locus_tag="VHS06_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEX2986942.1"
                     /translation="MERSRSWNNLGLATAAFALSFAVWSLLAPLASGIQKQYGLSDTQ
                     TGLLLAVPVLLGSLARIPMGIITDRLGGRITFTALMLFCIIPVVFVGLADSFGALLFW
                     GFWLGIAGSSFAVGVPFVSRWFPPGNQGTALGIYGMGNIGTALAAYLVPWIAGQYGWS
                     YAFWVFAPALAVAALLFWLLAEDAPITVRPVSLAESLEVVRQHRISWLFGLFYFVTFG
                     GFVAFSVFLPKLLVDWFGFAKTDAGFRTAGFVVLATVIRPMGGYLADRVGGARVLSWV
                     FTAAPVLGLVLAFEALGPNIVAVTGACLALAAAFGLGNGAVFKLVAQYFPRNTGIVTG
                     VVGAAGGIGGFFPPLVMGAVKDLTGNYALGLVLLALFTAFCLLMDYLVLLRRPISAEN
                     EHAEGRQRVKGRKSAA"
BASE COUNT          565 a          881 c          855 g          447 t
ORIGIN      
        1 agcgagatcg cgcggctgga tcgcaccatg gtgatagtcg cggcggatct cgctcacatg
       61 gggccctcgt tcggggatcc cgaaccgatg gatgcgacgg ccaaagtgag cctggctgct
      121 accgacgcgg agatgatggc cacgatttgt cgaggcgatg ccgagggctt cctcgcgatg
      181 ctgacggcgg agcaggacga gcgcaaggtg tgtggccttc cccccatcta cctggcgatg
      241 aagatgctgg gcgacgggat caccggcgag gtggtggact actccgtttg ccctgctcca
      301 gacgagtcag tcgtctcggt gactgggatc ctgctcctta cagcttagac ccagaggatc
      361 attaccgcga aggcggtgag cgcgaagacc gccgagtgac cgatctcccc catccccaac
      421 tgcttgaggg tcggctggta acctctgtga aggattgccc acaccgcctt ggcaaggaat
      481 ggggccagcg ccaggcccgc aaagggtggt gcccatccga cagtcgccga cgctatcagt
      541 agcgccagca cgacggccag gtagacgaag agatccctct ccgcggagcg tctcgggtcg
      601 gtcgccgtgg ccggccgctt gcgcgccggg gggcgataga acagtcgcac gtagaatacg
      661 ctggctccgg agtaaaggaa ccccagcagc cagacggcaa aggcccgatg gtccagcgtg
      721 ccggagccca cgtagtaagc cgcggggccc atggccgaca gggctgcgat ggataacagt
      781 cgagcgggcc agcttcggtc gagccttctt gccttcagtg gaagcatgat gctcagcgcg
      841 agggctgcca cggcccccag cggcagcaac gcccatcgat ggtatcgcag cagcagaaca
      901 gccgccgcga ctacccctat gcccgcatag atcgtcagtc gtccgtatgc cgcggttcgt
      961 cgatcctgcg cctcgctcga cttggttgga ccggtaagga cgaactccgc gggcctgctg
     1021 ctgatgaaca ggaacagcat cgagatgagc agcatgagag aggcgatacc ccatcccccc
     1081 aatgcgctcc ccaacaggta ggggacgagc cacatcgccc acgcgccgtg ctcgcgaggc
     1141 agcatcagag tcgcactcca gttgctgggc acgcgggcca tccgattttt cgtcgttgtg
     1201 ggtcattatg gctgacatac gatctcgtag gggcaggaga tggcgcctgt ccggcatatg
     1261 gcctcgcact cgccacagta gtcgcaatcg gtgtgcaata ggactacctt gcggttcttg
     1321 acctcaaggg cctttgccgg acagaccctg gtacaggcgc cgcatccgtt gcacttgctc
     1381 aagtcgatca caggaagggc cgggggtttg gactccacga tgctgtgcct cgattctgtt
     1441 ggttgaaagc ttgtttcacg ccgccgactt gcggcctttc accctttgcc tgccttcggc
     1501 gtgctcgttc tcggccgaaa tcggccggcg cagcaagacc aggtagtcca tcaggagaca
     1561 gaaggcggtg aagagtgcca gcagcactag ccccagcgcg tagttgccgg tcagatcctt
     1621 caccgcgccc atcaccaagg gcggaaagaa gccgccgatc cctcccgccg cgcctaccac
     1681 tcctgtgaca atgccggtgt tccttggaaa gtactgcgcc acaagcttga agacggcccc
     1741 gtttcccaac ccgaacgcgg cggccagggc aaggcaggcg ccggttaccg cgacgatatt
     1801 cgggcccagt gcctcgaagg cgaggaccag ccccaggacc ggagcggcgg tgaagaccca
     1861 gctcaacact cgggctccac ccacccgatc agccaggtat ccccccattg ggcggatcac
     1921 cgttgcgagc accacgaacc cggccgttct gaaccccgcg tctgtcttgg caaaaccgaa
     1981 ccagtctacc agcagcttgg gcaggaagac ggagaaggca acgaatccgc cgaaggtaac
     2041 gaaatagaag agcccgaaga gccacgagat tcggtgttgt ctcaccacct ctagactctc
     2101 ggccagtgag acaggtcgaa ccgtaatcgg ggcatcctcg gccaaaagcc agaagagtag
     2161 ggccgccacc gcgagtgccg gcgcgaacac ccagaaggcg taggaccaac cgtactggcc
     2221 cgcgatccat ggaaccaggt aggcagctag agcggtgcct atgttgccca ttccgtatat
     2281 gcccagggcg gtgccctgat tgcccggtgg gaaccacctc gacacgaagg gcacacccac
     2341 ggcgaaggaa ctgccggcta ttcccagcca gaagccccag aagagcagtg ccccgaagga
     2401 gtccgccaga ccgacgaaga cgacggggat gatgcagaac agcatcagcg ccgtgaaggt
     2461 gatccgcccg cccaaccggt cggtgataat ccccatgggg atgcgcgcta gcgagccgag
     2521 caggaccggg acagccaaca gcaggccggt ttgcgtgtcg ctgagaccat attgcttctg
     2581 tatccctgag gccaggggcg caagcagact ccacaccgca aagctgaggg caaaagccgc
     2641 ggtcgccagc ccgaggttgt tccacgacct cgacctttcc atgatggact ctcctctctg
     2701 gctgtctgct ttccgtcgct ggctaagctg gcagccacga cccccgaa
//
LOCUS       DATALS010000097         5453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_274517, whole genome shotgun sequence.
ACCESSION   DATALS010000097 DATALS010000000
VERSION     DATALS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274517"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VHS06_02075"
     CDS             complement(<1..585)
                     /locus_tag="VHS06_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986943.1"
                     /translation="MNSSSEGIRRRRLRVASQALAGLVLAIGCLVLIGWALNIHPLKS
                     PIPNMVSMKANAAIGFILVGLGLLLPSIGRSPSWNRAAQLFASITFLIGLVTIVEYIL
                     GINLFIDQLFFRDTDVVGTSVPGRMGPNTAIGFLLMGLALLLLLLPLQHGPSAAQLLA
                     AITGLIALAAILGYAFGAEDLYGFGRYTQMALHTA"
     gene            complement(805..1215)
                     /locus_tag="VHS06_02080"
     CDS             complement(805..1215)
                     /locus_tag="VHS06_02080"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX2986944.1"
                     /translation="MGVLRGLTLALSAGLILVWAVTRQGARGPRSVVDLSTDLRHGLQ
                     VGGIRWSGGFLRSMGAVESASRAELDALRAERIAHALTERLKDGAANVKAVIIGEIVH
                     LEGSVKSERERAEAESVAREVSGAQVTANDLRVE"
     gene            1344..3767
                     /locus_tag="VHS06_02085"
     CDS             1344..3767
                     /locus_tag="VHS06_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2986945.1"
                     /translation="MSGNEPRRSRDLLLHWLVGIAVGLAVLYVLLALLVGAGQVIAVL
                     PWYIPMMYSFAALAAFGIAFLAFGRYRVLREPAPFWIGMGFASFGILVLFYVLSWPGL
                     LPDGRGLIARLPNTSAGLLHVQFSALAVCLLAAALLPWPRAGARGERWWSLLVLTGIV
                     ASFAIAGLSVHFETVMPPLVLDGVWTPLHVVWFSFLLVAFAVGALLSSHRYLETGDAL
                     VGYVAMTQLVLTFFMLAGVISGELYDFWWYWQEILLVGGLSVMLFGLLSEYVGLYGRE
                     RERTRELEALQQVTDPALGRQGVESLLQSLLERIVGIMGAHAGAILLLDPQRQELILR
                     KGVGIAEEEAVGFRVAMGEDFAGRVAAENAVYWVRDAQADPSVWSPYIEAGHIRGMLG
                     APMRVDAEFTGVVHVDFLEAREFTRSEAHLLEVVAERAALAIRQAMLLQAAQDERNRL
                     RVLIDTAPIGIVFCTAPTGALALYNKASEVILGRSLAPQVGIPGRSVFTGAVHPDGKP
                     FAKGELPVSRSLRGETCIGVEVLVRQPSGRKVFILANCAPIRDPKGQVVGALVAFQDI
                     TPIREQEQLRDEFVSAAAHELKTPVTTIKGYAQLMHQWAPEGHEPREGKAVEVISAQA
                     DRINRRVQEMLEVIRYRKGPSDLHKVRFDLADLVSQVVQRIQSTTQIHQLLFSRDGPV
                     AVEADLEHIEEVVVSLLDNAIRYSPKGGEIEVRVWAQEQDAMVSVKDHGVGIAKERQP
                     HIFEPFYEPVPAGAPGYHSVVALSLYLSKLVVERNGGGIWFESEEGKGSTFYFRLPLA
                     QGGADGQHG"
     gene            3751..4125
                     /locus_tag="VHS06_02090"
     CDS             3751..4125
                     /locus_tag="VHS06_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2986946.1"
                     /translation="MASTDRAVLVVDDDAELLGMLQMILAEAGYRVITAGEGHEALEK
                     VEQELPGVILLDMKMPGMDGWEFAREFRARYGRSSPIVVLTASEDARERAEEIDAECH
                     VGKPFEIPDLLKVVESQLHTRA"
     gene            complement(4247..4334)
                     /locus_tag="VHS06_02095"
     tRNA            complement(4247..4334)
                     /locus_tag="VHS06_02095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4298..4300),aa:Ser,seq:gga)
     gene            complement(4425..4514)
                     /locus_tag="VHS06_02100"
     tRNA            complement(4425..4514)
                     /locus_tag="VHS06_02100"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4476..4478),aa:Ser,seq:cga)
     gene            4657..5004
                     /locus_tag="VHS06_02105"
     CDS             4657..5004
                     /locus_tag="VHS06_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986947.1"
                     /translation="MIIITPDAAKELRALAASGSAAVLQFDADVASDGSYDCSVGLVD
                     AVPADDSKVEVIDGISVVFRGMSESIFAGSIVGLNADGELTLEMAEGDCEGCEHGGDC
                     GCGEGGCGEGGCH"
     gene            5004..5408
                     /locus_tag="VHS06_02110"
     CDS             5004..5408
                     /locus_tag="VHS06_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HEX2986948.1"
                     /translation="MPLQPGRPGNDRPVVYDSDVGRVCPTCGQPEKSCRCRRKPAVGQ
                     AKQPQQSLSKPDGILRVSRDRSHRRGKTVTVITGAPGGPAAIDDLAGTLKRLCGSGGT
                     VKDGVIEIQGDHREQVLSKLLEMGFKAKQAGG"
BASE COUNT         1005 a         1552 c         1817 g         1079 t
ORIGIN      
        1 ggcggtgtgc agggccatct gcgtgtaccg gccgaagccg tacagatcct cggccccgaa
       61 tgcgtaaccg aggatcgccg ccagcgcaat caggcctgtg atcgcggcaa ggagttgagc
      121 cgcagatgga ccgtgctgca gcgggagcag cagaaggagc agggccaaac ccatcaggag
      181 gaagcctatg gcggtgttgg ggcccattct gcctgggacc gatgttccga ccacgtcagt
      241 atcccgaaag aagagttgat cgatgaacag gttgatgccg aggatgtact ccacgatggt
      301 caccagccca attaggaagg tgatcgacgc gaagagttgc gctgcgcggt tccaggatgg
      361 cgaacggccg atcgacggca ggagcaggcc cagcccgacc aggatgaagc cgatggccgc
      421 gttggctttc atcgagacca tattgggaat ggggctcttc aggggatgga tgttcagtgc
      481 ccagccgatc agcacaaggc aacctatcgc cagcaccagg ccggccagcg cctgggaagc
      541 gaccctcaat cgacggcgac gaattccctc gctactggaa ttcaacaatc ttgcaccaac
      601 ttttcgcggc caggaacagc aaggatggtt cgcactaagc gactttccct tgttggtatc
      661 cactcttcgc tcatggaaag gttgactgca tctagggaaa gccgcttagt ctggctggcg
      721 gccatccgtt gtgtctagga tgcatgatgc atgcgagcag acgcggataa gccggcccgg
      781 ataatggtgc gaggggcctt agccttactc gacccgcagg tcattcgcag tcacctgagc
      841 gccgctgacc tctcgcgcca cgctctctgc ctcagccctc tccctttcac tcttgacgct
      901 gccctccaga tggacgattt ctccgatgat gacagcctta acgttggctg ccccatcctt
      961 gagcctttcg gtcagggcgt gggcgatcct ctctgcgcgc aacgcatcca actccgcccg
     1021 cgacgccgac tcgacggctc ccatggaccg aagaaagccc ccgctccacc ggatgccccc
     1081 gacttgcaga ccgtgccgga ggtcggtact gagatcgact acggagcggg gaccgcgggc
     1141 accttgcctt gtcaccgccc atacgaggat gagccccgca ctgagcgcca gggtaagccc
     1201 tctcagcaca cccatggatg gtctctcctt cacctgcgat attgcggttt gctcggcaag
     1261 gtgtagacca cgtgtgatcg gcatgcctat tgcggaggat ggacgactac agtggccgca
     1321 gccacccaca atggaggtgg agagtgagcg ggaacgaacc ccgacggtcg cgtgatctat
     1381 tgctgcactg gctggtcggg attgccgtgg gcctggcggt gctctatgtg ttgctcgcgc
     1441 tcctagtggg ggcagggcaa gtcatcgccg ttctgccctg gtatatcccc atgatgtaca
     1501 gctttgcggc gctggcagct ttcggcatcg cctttctcgc cttcggtcga tatcgagtac
     1561 tgcgagaacc ggcccccttc tggatcggca tgggctttgc cagctttggc atccttgtct
     1621 tgttctatgt gctctcgtgg ccggggcttc ttccggatgg gcgaggtctg atcgccagac
     1681 ttcccaacac ctcggctggg ctccttcacg ttcagttcag cgcgctggcc gtgtgtctgc
     1741 tggcggccgc gctccttcct tggcctcgag caggagcccg tggcgagcgt tggtggtccc
     1801 tcctggtcct gaccggaatt gtggccagct ttgccatagc cggtctgtcg gtgcactttg
     1861 agactgtgat gcctccgctt gtgctggacg gggtctggac ccctcttcac gtcgtctggt
     1921 tctcgttcct tctggtggcg tttgcagtcg gcgccctgct atccagtcac cgttacctcg
     1981 aaactggcga cgctcttgtc ggctatgtgg ctatgacgca gctggtgctg acgttcttca
     2041 tgctggctgg tgtcataagc ggcgaactgt acgacttctg gtggtactgg caggagatcc
     2101 ttctggtggg cggtctctcg gtgatgctgt tcggactgct ctcggagtat gtggggctgt
     2161 acggtcgtga gcgagagagg acccgtgagt tggaggcgct gcaacaggtg acggatccgg
     2221 cccttggccg acagggggtg gaatcgctgc ttcagtccct tctagagcgg atagtgggaa
     2281 taatgggcgc tcacgcgggc gccatcctgc ttctcgatcc acaacgacag gagttgatac
     2341 tgaggaaggg tgtagggatt gccgaggagg aggcggtcgg ctttcgggtg gccatgggtg
     2401 aggactttgc ggggcgagtg gctgcggaga acgccgtcta ctgggtgcgg gatgctcagg
     2461 cagatccctc cgtctggagc ccgtacatcg aggcaggcca catcagaggc atgttgggcg
     2521 ccccaatgcg cgtcgacgca gagttcaccg gtgtcgtaca cgtggacttt ctggaagcca
     2581 gggagtttac ccgcagtgag gcacatcttc tggaggtggt ggcggagcgg gcagcgctgg
     2641 ccatccggca ggcgatgctc ttgcaggcgg cacaggacga gcgcaatcga ctgcgagtgc
     2701 tgatagacac cgcaccaatc ggtatcgtct tctgtacggc gcccaccggc gccctggcgc
     2761 tctacaacaa ggcatccgag gtcatcctgg gcaggtcctt ggcgccgcag gtgggtatcc
     2821 ccggcaggtc tgtgttcacc ggtgccgttc atcccgacgg caaaccgttc gccaaaggcg
     2881 aattgccggt tagtcgatcc ctgcgcggcg aaacctgcat aggcgtggag gtgttggtgc
     2941 gtcaaccctc cggccgcaag gtcttcatcc tggcaaactg tgcaccgatc cgcgacccca
     3001 aggggcaggt tgtgggtgcc ctggtggcgt tccaggacat cacccccatc agggagcagg
     3061 agcaactccg agacgagttc gtctcggctg ccgcccatga actaaagact cctgttacca
     3121 caatcaaggg gtacgcgcag ttgatgcacc agtgggcgcc ggaaggtcac gagccgcggg
     3181 aggggaaggc cgtggaggtg atcagcgccc aggccgaccg gatcaatcgt cgggtgcagg
     3241 agatgctgga ggtcattcga tatcgaaagg gtccttccga cctgcacaag gtgcgcttcg
     3301 acctggccga cctggtctcg caggtggtgc agcggataca gtcaaccacc cagatccatc
     3361 agctgctctt ctctcgcgac ggaccggtgg cggtggaggc cgacctcgag cacatagagg
     3421 aggtggtagt cagcctgctg gacaacgcca tcaggtattc cccgaagggt ggcgagatcg
     3481 aggtgcgggt gtgggctcag gagcaggatg cgatggtttc ggtgaaggac catggggtgg
     3541 ggatcgcgaa ggagcggcag ccccacatct tcgagccgtt ctatgaaccg gtgccggcgg
     3601 gtgctccggg ttaccacagt gttgtcgctc tcagcctcta cctcagcaaa cttgtcgtgg
     3661 agcgcaatgg tgggggtatc tggttcgaga gcgaggaggg caaggggtcc accttctact
     3721 tccgcttgcc gttggcccag ggaggtgccg atggccagca cggatagagc ggtgctggtg
     3781 gtggacgatg atgccgagct tctcggcatg cttcagatga tcctggcgga agcgggctat
     3841 cgggtgatca ccgcagggga ggggcatgag gcgctggaga aggtggagca agagttgccg
     3901 ggcgtaattc tgctggacat gaagatgccc ggcatggacg gctgggaatt cgcccgtgag
     3961 ttccgtgcca ggtacggccg atcgtctccg atcgtggtgt tgactgcttc agaagatgcc
     4021 agggagcggg cggaggagat cgatgccgaa tgtcacgtgg gcaaaccgtt cgagatcccc
     4081 gacctgctca aggtggtgga gagtcaactg cataccaggg cttgaccatc agcatggccg
     4141 acacgacggc atgtgatgag gagagaaaaa cggccaccat acttggtatg gtggccacgc
     4201 ttttgcgcat gtcccgacga gcaacagatc cggggcagtc tcgatatggc ggagagggtg
     4261 ggatttgaac ccacgaggct gttacaccta cccgctttcc aggcgagcgc cctaggccac
     4321 taggcgacct ctccaaacaa gctatcacct atgagttatc agctatcagt cccctctatc
     4381 cgcatgttgc tgatagccga cggctgatag ctgaccacta tttgtggcgg agggagaggg
     4441 atttgaaccc ccgaggcctt gcggcctgct cgatttcgag tcgagtgcac tcaaccggac
     4501 tatgcgatcc ctccagaaag cacagaccgc cgattgcagc ggccacgagt tggaagtata
     4561 gcacatgccc ggcatggcga caatgagatt cgttcttggt atacttcccg gaccactccg
     4621 ggtcacagtc ggcggtttca caatgaggag gatgaattga ttattatcac gccggacgct
     4681 gccaaggaac tgcgtgccct cgctgccagc ggaagtgccg ctgtcctaca gttcgatgcc
     4741 gacgttgcat ctgatgggag ctacgattgc tcggttgggc tggtggacgc cgtgcctgcg
     4801 gatgactcca aagtcgaggt gatcgacggc atcagcgtcg tcttccgagg catgtccgag
     4861 agcatttttg ccggctccat cgtcggcctc aacgcggacg gagagttgac cctcgagatg
     4921 gccgagggcg attgtgaggg ctgcgagcac ggcggcgact gcggctgcgg tgagggcggt
     4981 tgcggcgagg ggggctgcca ctgatgcccc tccagcctgg gcgtccgggg aacgatcgac
     5041 cggtcgtgta cgacagcgac gtcggtcgtg tctgccccac ctgtggacag cccgagaaga
     5101 gctgccgctg ccgtcgcaag cccgcggtcg ggcaggcaaa gcagccgcag cagagcctct
     5161 cgaagccaga cgggatcttg cgcgtttctc gtgatcgcag ccaccgcagg ggcaagacgg
     5221 tcacagtgat cacgggtgcg ccgggcggac cggcagcgat cgacgacctg gcaggaacgc
     5281 tgaagcggct gtgtgggtcg ggagggacgg tgaaggatgg ggtgatcgag atccagggcg
     5341 atcaccgcga gcaggtcctg tcgaagctgc tggagatggg gttcaaggcg aagcaggccg
     5401 gcggctgagg ccagtagggg cgatgcattt atcgccacga gtgtacaagt tgt
//
LOCUS       DATALS010000098         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_8919,
            whole genome shotgun sequence.
ACCESSION   DATALS010000098 DATALS010000000
VERSION     DATALS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8919"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VHS06_02115"
     CDS             <1..867
                     /locus_tag="VHS06_02115"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX2986949.1"
                     /translation="DLAGSCFCASFRCSCQLCYASFCCSFVHPKAVPQLADADELMSK
                     IERPAGVEFRVLVPNMRGLQRAIGHKPKRMNFMMSVTESHNRSNANCSVEESLKGFEP
                     MAAMALEAGIQPVGGMACTFGCPFEGEVPVERLEGITRRYMAMGIREIGLSDTVGVAK
                     PLQVYTVAARMLDRFPDVQWNLHLHNTRDMALPNVFAAMQAGMTNFESSIGGLGGCPY
                     APGATGNVATEDLVNMLTEMGIETGVDLDALIAIAKRLEEIIPHRLDSCMVRAGKRTD
                     LKPAPKEQQKLA"
     gene            1007..2092
                     /locus_tag="VHS06_02120"
     CDS             1007..2092
                     /locus_tag="VHS06_02120"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX2986950.1"
                     /translation="MARRSGLWVCLVAVLALSFTATACGGQASAPAKPAAEPTKASAS
                     APAATTAPAAAATTAPAAPKVDYPPKTVTFIAPSKPGSGFDTTARAIAATLEKEKMIT
                     FALPVQNASSPPEGMATIVQQYKGKAEMINVGSITTFLNKASGASPYGHKDLTPVAAL
                     LSTYYGVVVRADSPYKTLTDLLKDLKEKPDQTPLCGGQSDDRIAYGALALAYGADLKK
                     INYAAYAGGTEASAVLLEGSAKAEITTIDDIMGLIESKKVRALAVSGSQRLSGVLADV
                     PTFKEAGVNLEWANFRFIAGGPQMPDYAVKYWQDMLGKMVKTQTWKDNMAKYRWVDNF
                     STTDLGKYMDDKAAIIDKVAAELGLKQ"
     gene            complement(2302..2475)
                     /locus_tag="VHS06_02125"
                     /pseudo
     CDS             complement(2302..2475)
                     /locus_tag="VHS06_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
     gene            complement(2490..2954)
                     /locus_tag="VHS06_02130"
     CDS             complement(2490..2954)
                     /locus_tag="VHS06_02130"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX2986951.1"
                     /translation="MSFDRGLSIGLFLFSSWALYLSITMPKTAIRQTVGPEVFPIVIS
                     FCMILAAVALFVKTVREKATKVRHSELPEGVGEDRLTQGLALLGIGVYIFAMEPLGYI
                     VSTALLCIYETAVFETGHWVRNVASGVGFSVVVYATFVSFLDVMLPKGILGW"
BASE COUNT          628 a          959 c          947 g          500 t
ORIGIN      
        1 gaccttgccg gttcttgctt ctgtgcctcc tttcgatgta gctgtcagct ttgttacgcc
       61 agcttctgct gctccttcgt tcaccccaag gccgttccgc agctggccga cgccgatgaa
      121 ctgatgtcga agatcgagcg accggcgggg gtggagttca gggtcctcgt gcccaacatg
      181 agggggctcc agcgagccat cggccacaag ccgaagcgaa tgaacttcat gatgtccgtc
      241 accgagtccc acaaccgctc caacgccaac tgctccgtag aggagtcgct caagggcttc
      301 gagccgatgg cggccatggc gctggaggcc ggaatccaac cagttggggg gatggcctgc
      361 accttcggct gccccttcga gggagaggtg ccggtggagc ggctggaggg gattacccgg
      421 cgctacatgg cgatggggat cagggagatc ggcctatcgg acacggtggg cgtcgccaaa
      481 cctcttcaag tctacaccgt ggcggctcga atgttggaca ggttcccgga cgtgcagtgg
      541 aacctgcacc tccacaacac ccgcgacatg gctcttccca acgtattcgc ggccatgcag
      601 gccggcatga ccaacttcga gtcctccatc ggcggcctgg gcggctgccc ctacgccccg
      661 ggcgccaccg ggaacgtggc caccgaggac ctggtgaaca tgctcaccga gatggggatc
      721 gagaccggag tagacctcga cgccctcatc gccatcgcga agaggctgga ggagatcatc
      781 ccccaccgat tggacagctg catggtgaga gccggcaaac ggacggatct gaagccggcg
      841 ccgaaggagc agcagaagct ggcgtaacaa agctgacagc tacatcgaaa ggaggcacag
      901 aagcaagaac cggcaaggtc cccccaaggc agaactccgg aacaaggtcg agctaaaggc
      961 cccgcatcca tcgagtagaa atgtacgatc ggacaaggag gtcaccatgg ctaggcgatc
     1021 tggactctgg gtatgcctcg ttgccgtcct ggcgctttcc ttcacggcaa ccgcgtgcgg
     1081 aggacaggcg tccgcacccg ccaaacccgc cgccgagcct accaaggctt cggcttccgc
     1141 tccggcggcc acaacggccc cggccgcggc ggctacgacc gcacccgcgg ctcccaaggt
     1201 ggactaccca ccaaagacgg ttaccttcat cgccccgtcc aagccgggca gcggcttcga
     1261 caccaccgct cgcgccatcg ccgccaccct cgagaaggag aagatgatca ccttcgccct
     1321 tccggtgcag aacgccagct ctccccccga gggcatggcc accatcgtgc agcagtacaa
     1381 gggcaaggcc gaaatgatca acgtcggctc catcaccact ttcctcaaca aggctagtgg
     1441 tgcgtcccct tacggccaca aggatctcac ccccgtcgcg gccctgctct ccacctacta
     1501 cggcgtcgtc gtgcgggcgg actccccata caagaccctg acagatctgc tcaaggatct
     1561 caaggagaag ccggatcaga ccccgctgtg cggtggccag tccgacgacc gcatcgcata
     1621 cggcgctctc gccctggcct acggcgcgga cttgaagaag atcaactacg ccgcctacgc
     1681 cggcgggacc gaggcgagcg cggtgctgct tgagggttca gccaaggccg agatcaccac
     1741 catcgacgac atcatgggac tgatcgagtc caagaaggtc cgggcgctgg cggtcagcgg
     1801 cagccagcgg ctgtccggcg tgctggcaga cgtccccacc ttcaaggagg ccggggtgaa
     1861 cctggagtgg gccaacttcc gcttcatcgc cggcggacct cagatgccgg actacgccgt
     1921 caagtactgg caggacatgc tgggcaagat ggtcaagacc cagacctgga aggacaacat
     1981 ggccaagtac cgctgggtcg acaacttctc caccactgac ctcggcaagt acatggacga
     2041 caaggccgcg attatcgaca aggtggcagc cgagctgggt ctcaagcagt agcttcggga
     2101 ggtgtcgggt gtcgggtgcc aggggactgg tgtcgggtga tcggggacgg gaacacggct
     2161 ggagccgata cccagcaccc aatacccgat acctagcacc cgatacccga catccggcac
     2221 ccgatacctg gcacccgcaa ggaggttcca ttatgagttt cgaccgaggc ctatccatcg
     2281 gcctcttcct cttctcatcc tggggctcat gttgtaggtg gcaggaagca ggatggcgat
     2341 ggctgtgatg gggccgatgc ccggcagaac cccgatgacg gtgccaagca ccgcaccgac
     2401 gaagcagtag aagacgttct ctggggtgag ggcgataccg aagcccaggg cgacgttgtt
     2461 gaagaactga tccatctact ttactcccct caccatccca ggatgccctt gggcagcatc
     2521 acgtccagga agctgacgaa ggtggcgtag acgacgacgc tgaagccaac cccgctggcg
     2581 acgttgcgca cccagtggcc ggtctcgaaa acagcggtct cgtagatgca caggagcgcg
     2641 gtcgatacga tgtagccgag gggctccatc gcgaagatgt agacaccgat gccgaggagc
     2701 gccagcccct gagtgaggcg gtcctccccc accccctcgg gcagttcgga gtgtcgaacc
     2761 ttggttgcct tctccctcac ggtcttcacg aatagggcaa ccgcggccag tatcatgcag
     2821 aaggatatga ctatggggaa cacctccggc cccacggtct ggcgaatggc ggtcttgggc
     2881 atggtgatgg ataggtagag cgcccaggat gagaagagga agaggccgat ggataggcct
     2941 cggtcgaaac tcataatgga acctccttgc gggtgccagg tatcgggtgc cggatgtcgg
     3001 gtatcgggtg ctaggtatcg ggtattgggt gctg
//
LOCUS       DATALS010000099         7645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_143222, whole genome shotgun sequence.
ACCESSION   DATALS010000099 DATALS010000000
VERSION     DATALS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143222"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VHS06_02135"
     CDS             complement(<1..732)
                     /locus_tag="VHS06_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX2986952.1"
                     /translation="MLLATCGIDPMRAIPRFTFGIPDVEAGFDFLTVLIGLFAFSQLM
                     GDLEKLRNPDKVERIENPDTHIPTIRVLKDIWDNKVGMVWASLVGLWIGIVPAAGGAV
                     ANVLAYDQAKKVSKHPEKFGTGYHEGIIASEAGNNSVSAGDLVPTMALGIPGDAVTAV
                     MLGALLIHGLNPGPLLMTTQPKLAYGLICAYLIATVLTLVIQLGLMRILVKLILVPKN
                     ILIPGILVMSALGAFVLNNRIFDIWV"
     gene            1105..2364
                     /locus_tag="VHS06_02140"
     CDS             1105..2364
                     /locus_tag="VHS06_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2986953.1"
                     /translation="MKRIATLGLATLVLAMTALLFVAGCSSSAPAASPTKAAEPAKSG
                     STAAPTAAPAAGATSAPAAASASGKAVAVKVGLITPLSGDVKTYGESVKNSVELAIDE
                     ANKAGKVQISTVIADSKGDPTEGVNAFTKLANQDKVKAIIGPVISRVAIGVSESAQAA
                     KVLMITPTGTAPKVTVDGGKRKDYVFRSCFIDPFQGEVMSKFALTTLKSKTAAVVYDI
                     SNDYSKGLAETFKTAFEKGGGKVVGYESYGKDDVDFSAILTKIGSSNADALFLPDYYN
                     RVNLIAKQAKEKGIKSVLLGVDGWDSAELDTKATEGGYFSNHYSADDTRPEVQAWTKK
                     YQEKFKSVPDALATLGYDGTNLLVEAITKAGSDDSTKIRDAMAAIKDYKAVTGTLSFD
                     KDGNPIKSAVVLQIKDGKQKYVQTVNP"
     gene            2987..4237
                     /locus_tag="VHS06_02145"
     CDS             2987..4237
                     /locus_tag="VHS06_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX2986954.1"
                     /translation="MRLVYQDWRTDWFPPMARELLDQFHATHPNISVFYLPDQAGTAT
                     RYFNKMLEEMQSGVAADVFQGCCAHFPIWAQKGYTLDLRPYVKADLDQATIDDWDLAQ
                     YNSFFTREGKQYGLPKYHGALALYYNKDILDRYRVSYPDRSWSYDDYLAAMRKLTQDR
                     DGDRRTDLWGSMLDVSWDRMQVHINGFGGRFVDPADPSRCRMADAPALKALEWLRARM
                     QDDRVMATVRDVENVGITQTFIDQRVAMVEDGSWSLKNILSGARFRVGVAPFPTAPVR
                     RVTLSSSDGFGIYSGTRHPEAAWELVKFLISKEYGRAMARANFLQPARASLVDEWVTY
                     VRNQFPWESEGVDIAAFADGQLNGYSVTAEFFPNMAEAQQIALDAWDKILTLGQAPLE
                     YMVSAAEQIQEAQNGESSGGQSCC"
     gene            4228..6978
                     /locus_tag="VHS06_02150"
     CDS             4228..6978
                     /locus_tag="VHS06_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2986955.1"
                     /translation="MLLNVSLKGLRVKIIAWSFIPTAIILLAAAMVTFYAYQGVTEDL
                     VLRRSQELIRLSAGQLSADMMEYADLLSGMARTSDVYGNDPMMQQRALSSSRDRLVVF
                     DAGVLILNSGGKVVAAEPARPDLFARTLSDRGYFAQMLRTETPVFSDVITDGRDGRDV
                     IVVAVPILGERGEFRGAMAGMFYLGATSVSTFYGGIVKLRLGENGNTHLVDGNRKIVY
                     DSEPVRIGVRFPDGVVGQQLAERKVGDLRTRDENGQEIVAGFAPVAGTPWSLVVEERW
                     DLLMGPSQSYQRFLLLLLALGVVVPAVVVMVAMGRITGPIALLTKGAQEVAGGNFGRK
                     VSVSTGDEIEELAKQFNLMSSQLQEYTSNLEKRVADRTRELAALNDVAAVVSCSLNLK
                     EILEGALEKSLQTMGLDGGGIFLLDERRGVLSIAAQRGLDPTLVEAIDNLKLGEGFSG
                     RVVQLGEPLVVRDLVNDPRLTPSVPRDSAQHSIASVPLTSRGKVVGALFVGSRVDRHF
                     SQQDLQLLCSIGQQIGVAVENARLFASEQRRAEQFRVISEVGHRITSILSVEELLHEM
                     VSLVMETLGYHMVGIGLIEGDRVVYRAGAGPGWVARGHSQLRLKIGEEGITGWVAAHG
                     EPLLVPDVTKDPRFMKVAEEDGARSELAVPLKTHEDVIGVLDVQSERLNAFDSSDLGV
                     LQTLAHQAAVAIENARLYERAQQLAVMEERNRLARDLHDSVTQAVYGVTLYAEAATRL
                     LAAGQVEVAASHLQELRITAQQALREMRSLIFELRPPVLEQEGLVAALQARLESVEGR
                     AGIGTELKIDGECPLPKETEEGLYRIAQEALNNVLKHSEAHNVRLYLQRKDGNALLEV
                     SDDGVGFDPLEDREHGGLGLGSMQERAALLGARLTITSNPGGGTTVRVEVSSCGVTKG
                     EE"
     gene            6979..7641
                     /locus_tag="VHS06_02155"
     CDS             6979..7641
                     /locus_tag="VHS06_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2986956.1"
                     /translation="MADATIRVMIADDHSIVRKGIRALLATESDIEVVGEAADGVQAV
                     AQAERLQPDVILMDMVMPQMDGVEAIRRIIARRPGAHILVLTSFAADEKVFPAIKAGA
                     LGYLLKDSGPEELVHAIHQVHLGQSSLHPTIARKLLQEISHSSGKPTIPDPLTDREIE
                     VLKLVAKGESNRQIADTMVISEATVRTHVSNILGKLHLASRTEAALYALREGIASLED
                     SL"
BASE COUNT         1444 a         2256 c         2502 g         1443 t
ORIGIN      
        1 cacccagata tcgaatatgc gattgttgag gacgaaggcc cccagcgccg acatgaccag
       61 gatgccgggg atcaggatgt tcttcggcac caggatcagc ttcaccagga tccgcatcag
      121 ccccagctgg atgaccagcg tcagcaccgt ggcgatcagg taggcgcaga tcagtccgta
      181 ggccagcttc ggctgggtgg tcatcaacag cggtcccggg ttcagcccgt gtatcagcag
      241 cgcgcccagc atgacggccg tcaccgcgtc gcccggtatc cccagcgcca tggtgggcac
      301 caggtcgccg gccgaaaccg agttgttgcc cgcctccgaa gcgatgatcc cctcgtggta
      361 cccggtgccg aacttctccg ggtgcttgga cacctttttg gcctggtcgt aagcgagcac
      421 gttggcaacc gcgccgccgg cggcggggac gataccgatc cacagcccca ccagtgacgc
      481 ccagaccatt cccaccttgt tgtcccagat gtccttcaac acccggattg tcgggatgtg
      541 ggtgtcaggg ttctctatcc gctccacctt gtccgggttt cgcagctttt ccaagtcgcc
      601 catcagctga gagaaggcga agagtccgat cagcaccgtc aggaaatcga aacccgcttc
      661 tacgtcggga atgccgaagg tgaagcgcgg gattgcccgc atggggtcta tgccgcaggt
      721 ggccagcagc atgccgaggg aatgggaatc tatatcgctt gttcaaaatg ataggtttgt
      781 cggctaccgg cgtcaatact acctgaacta tgtcgatctc ctcctcgctt gacacgggtt
      841 atggatcggg actagactgc gcacaatctg atgctggccc agatgttgag tgcccctctt
      901 ctatagcgct tgccgaaccg gtaggtgaga cagacccttc cggaacagat cggcgggcgt
      961 cttgcggttt ctcacacctg ggtgtccgct catcttctac aatcccagtg gctttttcta
     1021 aagggtcgct ctacttgagc tgtgcgtctt ggcactcctc tgcgattagg ggtgcccgca
     1081 gataacttta cgaggagaac cgcaatgaag cgcatcgcaa ccctcgggtt ggcgacgctg
     1141 gtgctggcga tgactgccct gctgtttgtg gcaggctgct cgtcgtctgc gcccgccgcc
     1201 tccccgacca aggctgccga gcctgccaag tctggctcga ccgcagcccc caccgccgca
     1261 cccgcggccg gggcaacctc cgcaccggca gcagcgtctg cttccggcaa ggccgtcgcc
     1321 gtcaaggtcg gtctcatcac gccgctctcc ggcgatgtga agacctatgg cgagtccgtc
     1381 aagaactccg tggaactcgc gatcgacgag gcgaacaagg ccggtaaggt ccagatcagc
     1441 accgtcatcg ccgacagcaa gggtgatccc accgaagggg tcaacgcctt caccaagttg
     1501 gcaaaccagg acaaggtcaa ggcgatcatc ggcccggtga tctctagggt cgccatcggc
     1561 gtctccgagt ccgcccaggc cgctaaggtc cttatgatca ccccgaccgg cacggccccg
     1621 aaggtcaccg tggatggtgg caagcgcaag gactacgtct tccgctcctg cttcatcgat
     1681 ccgttccagg gcgaggttat gtccaagttc gcgctgacca cgctgaagtc gaagaccgcg
     1741 gctgtcgtgt atgacatcag caacgactac agcaaggggc tggccgagac ctttaagact
     1801 gccttcgaga agggtggagg aaaggtcgtc gggtacgagt cctacggcaa ggacgatgtg
     1861 gacttctccg cgatcctgac caagattggc tccagcaacg ccgacgcgct gttcctgccc
     1921 gactattaca accgtgtcaa cctgatcgcc aagcaggcga aggagaaggg tatcaagtct
     1981 gtgctgctgg gcgtggacgg ctgggactcc gccgaactgg ataccaaggc caccgagggc
     2041 ggctacttca gcaaccacta cagtgccgac gacacccggc ccgaggttca ggcctggacc
     2101 aagaagtacc aggaaaagtt caagtcggtt cccgatgccc tggcgaccct tggctacgat
     2161 gggaccaacc tgctggttga ggccataacc aaggccggta gcgacgattc caccaagatc
     2221 cgcgacgcca tggccgcaat caaggactac aaggccgtga ccggcaccct ctcctttgac
     2281 aaggacggca acccgatcaa gagcgcggtc gttctccaga tcaaggacgg caagcagaag
     2341 tacgttcaga cggtgaaccc gtagtctagt tctccgtcat agcgattgtg tttcttgtag
     2401 gggcgacgca gacgtctgcg tcgcccctac gcatgcccgg acgctgcaat tgtgtgtaac
     2461 cgtactgtaa cggcggaact atcagacaac catgggaatc gccgatctat cttagtgagg
     2521 gttgcgacgg tgagtcgacc gagtccccag ttggcaacag aaacgtgcca gggcgctccg
     2581 atgagccggc ccgtctgata tccctcccga tgcccgcggg gatgctcccg cgggcatcgt
     2641 tcatttcgcc ctgtcttgag cttgattgca tgaggggcgg ccaggtacac ttgcggtggt
     2701 gctggagtcg ccgggagcag cacttcgtgg atgtttggtg cgccctacgc atcccgtcgt
     2761 tgcgtgggcg gatcaggcat tcggcgctat ctgagcggtg aaggactgga cccccatgca
     2821 ggttgatcag ccgaatcaga cttccggccc ccgctgtgcg cgaggcgcgg gccgggccct
     2881 cacgcggcgc agttttttgc ggcttgccgg cgcgagtttg gctgccgctg ccctcacatc
     2941 ctgtagcctt ccacccccca aaccgacgcc gaggaccggc gagaaggtgc ggctcgtcta
     3001 tcaggactgg cgcaccgact ggtttccccc tatggctcgc gaactcctgg accagtttca
     3061 cgccactcat cccaacatca gcgtctttta ccttcccgat caggccggta ccgccactcg
     3121 atacttcaac aagatgttgg aggaaatgca gtcgggggtc gcggcggacg tcttccaggg
     3181 ttgctgcgcc cacttcccca tctgggctca gaagggctat accctggacc tgcgccccta
     3241 cgtgaaggcc gacctggacc aggctaccat cgacgattgg gacctggcgc agtacaactc
     3301 gttcttcacg agggagggca agcagtatgg ccttcccaag tatcacggtg ccctggctct
     3361 ctactacaac aaagacatcc tggatcgata cagggtgtcc tatcccgatc ggagctggag
     3421 ctacgacgac tacctggctg cgatgcggaa gttgacccag gatcgagatg gcgaccgaag
     3481 gactgatctg tggggcagca tgctggatgt gtcgtgggat cggatgcagg tccacatcaa
     3541 cggtttcggg gggcgcttcg tggaccccgc ggaccccagc cgatgtcgca tggccgacgc
     3601 tcctgccttg aaagcgctgg agtggcttcg agctagaatg caggatgaca gggtgatggc
     3661 caccgtccgg gacgtggaga acgtcggcat tacccagaca ttcatagatc agcgtgtggc
     3721 tatggtcgag gatggctcct ggtcgctcaa gaatatcctt tccggtgcgc gcttccgagt
     3781 cggggttgca cccttcccga cggccccggt gcgcagagtc accttgagca gttcggacgg
     3841 cttcgggatc tactccggaa cccgccaccc agaggcggcc tgggagttgg tgaagtttct
     3901 gatcagcaag gagtatggac gggcaatggc ccgggccaac ttcctgcagc ctgccagggc
     3961 atccctggtg gatgaatggg tcacctacgt tcggaatcag tttccatggg agagcgaggg
     4021 agtggatatc gcggccttcg cggacggtca gttgaacggc tactcggtga cggccgagtt
     4081 cttccccaac atggcagagg cacagcagat cgccttggat gcgtgggaca agatcctgac
     4141 ccttggccag gcccccctgg agtatatggt ctccgcggcg gagcagatcc aggaggctca
     4201 gaatggggag agttctggag gccaatcgtg ctgctgaatg tctccctgaa gggcctcagg
     4261 gtcaagatca tagcctggtc cttcattcca accgcgatca tccttctggc ggcggctatg
     4321 gtcaccttct atgcctacca gggggttacc gaggatctgg tgctgagacg gagccaggag
     4381 ttgatccgcc tctcggcggg acaactctct gcggatatga tggagtacgc ggatctcctc
     4441 tccgggatgg cgcgaacttc cgacgtctac ggcaacgacc ccatgatgca gcagcgcgcc
     4501 ctcagcagtt cgcgtgatcg cctggtggtg ttcgatgcgg gcgtcctgat cctgaacagc
     4561 ggaggaaagg tcgtggcggc cgaacctgcg cgcccagacc tgttcgcccg aaccttgtcc
     4621 gaccgcggct acttcgcgca gatgctacgc acggaaacgc cggtcttctc cgacgtcatc
     4681 accgacgggc gggatggtcg ggatgtcatc gtggtggcgg tgcccatcct tggggagcgg
     4741 ggcgagtttc gaggggccat ggccgggatg ttctatctgg gcgcaaccag cgttagcacc
     4801 ttctatggtg gcatagttaa gcttcggctg ggagagaacg gcaacaccca cctggtggac
     4861 gggaacagga agatcgtcta tgactccgag ccggtccgga tcggcgtgcg cttccctgat
     4921 ggggtggttg gccagcagtt ggcggagcgg aaggtgggcg atctgcgcac ccgcgacgag
     4981 aatggacagg agatagtggc cggcttcgct ccggtcgccg gcaccccgtg gagtctggtg
     5041 gtagaggagc ggtgggacct gttgatgggc cccagccaga gctaccagcg cttcttgctt
     5101 ctgctgctcg ccctgggcgt ggtggtgccg gctgtggtgg tgatggtggc aatggggcga
     5161 atcaccggcc cgatcgcact gctcaccaag ggtgcacaag aggtggccgg agggaacttc
     5221 gggcgcaagg tctccgtctc cacaggggat gagatcgagg aactggcgaa gcagttcaac
     5281 ttgatgtcgt cgcagcttca ggagtacacc tccaatctgg aaaagcgggt tgccgaccgc
     5341 accagggaac tggcggctct caacgacgtt gccgcggtcg ttagctgctc cctgaacctc
     5401 aaggagatcc tggagggcgc gctggagaag tcgcttcaga ccatggggct ggacggcggt
     5461 gggatcttcc tcttggacga gcgacgcgga gtgctgagca ttgccgccca acggggactg
     5521 gatcccacac tggttgaggc catcgacaat ctcaaactcg gtgagggctt ctctggtagg
     5581 gtggtccagt tgggcgaacc gttggtggtg cgggacctcg tcaacgaccc gaggctgacc
     5641 ccgagcgtgc ccagggacag tgctcagcac tccattgcca gcgttccgct gacctcgagg
     5701 gggaaggtgg tgggtgcgct attcgtcggc agccgagtcg atcgccattt ctcccagcag
     5761 gatctgcagt tgctctgctc catcggacag cagatcggtg tggctgtgga gaacgctcgc
     5821 ctttttgcgt cggaacagcg tcgagccgag cagttcagag tgatcagcga agtgggacac
     5881 cggatcacct cgatcctttc tgtggaggag ctgcttcacg agatggtcag cctggtgatg
     5941 gagacccttg gctaccatat ggtggggatc gggctgatcg agggcgacag agtggtgtat
     6001 cgggccgggg cgggcccagg ttgggtcgcc cgagggcact ctcagctgag gttgaagatc
     6061 ggcgaggagg ggatcacggg ttgggttgcc gcccacggcg agccgctcct ggttcccgac
     6121 gtcaccaagg atcctcgctt catgaaggtc gccgaggagg acggtgcacg ttctgagctg
     6181 gccgtgccgt tgaagacgca cgaggacgtg attggcgtgc tggatgtcca gagcgagcga
     6241 ctgaacgcat tcgatagcag cgatctcggc gttttgcaga cccttgccca tcaggcggcc
     6301 gtggcaatcg agaatgcgcg gttgtacgag cgcgctcagc agttggcggt gatggaggag
     6361 cggaaccggc tggccaggga cctgcacgac tccgtcactc aggcggtgta tggggttacc
     6421 ctctacgccg aggcggccac gaggttgctg gcggcgggac aggtggaggt cgccgcgtcc
     6481 cacctgcagg agttgcggat cacggctcag caggcgctgc gtgagatgcg ctccttgatc
     6541 ttcgagttgc gaccgccggt gctcgagcag gaggggcttg tggctgcgct gcaggctcgg
     6601 cttgagtcgg tggaggggcg agcggggatc gggacggagt tgaagatcga tggggagtgc
     6661 ccgctcccta aggagaccga agaggggcta taccggatcg cccaggaggc gctcaacaac
     6721 gtcctgaagc actccgaggc gcacaacgtc aggctctacc tgcagcggaa ggatggcaac
     6781 gctctcctgg aggtgtcgga cgatggcgtc ggtttcgacc ccctggagga tcgagagcac
     6841 ggtgggctgg gcctggggag catgcaggag cgagccgcct tgctcggtgc gagactgacc
     6901 atcaccagca acccgggtgg aggcacgaca gtccgggtgg aagtgagttc ctgcggcgtt
     6961 acgaaggggg aggaatagat ggctgatgcg acgatacggg taatgatcgc ggatgaccac
     7021 agcatcgtcc ggaaggggat ccgcgccttg ctggcgacag agtcggacat tgaggtggtc
     7081 ggggaggcgg ccgacggcgt gcaggcggtg gcgcaggcgg agagactgca gcccgacgtg
     7141 atcctgatgg acatggtgat gccccagatg gatggcgtgg aggcgatccg caggatcatc
     7201 gcccgcaggc ccggggcgca catattggtg ctgaccagct ttgccgccga cgaaaaggtg
     7261 tttcctgcca tcaaagcggg tgcgctgggt tatctgctga aggactccgg acccgaggag
     7321 ctggtacatg cgatccacca ggtgcacctc ggccagtcgt ccctccaccc caccattgcc
     7381 agaaagctgc tgcaagagat ctcccactcc tccggcaagc cgaccatacc cgatcccttg
     7441 acggaccggg aaatcgaggt gctgaagctg gtggccaagg gggagagcaa tcgccagatt
     7501 gccgacacca tggtgatcag cgaggcgacg gtgcgaactc acgtgagcaa tattctgggc
     7561 aagctgcacc tcgcatcgcg caccgaggct gccctctacg ccctgcggga gggtatcgcc
     7621 tccctggagg actccctgta gcccc
//
LOCUS       DATALS010000100         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_234639, whole genome shotgun sequence.
ACCESSION   DATALS010000100 DATALS010000000
VERSION     DATALS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234639"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VHS06_02160"
     CDS             <1..766
                     /locus_tag="VHS06_02160"
                     /inference="COORDINATES: protein motif:HMM:NF045129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX2986957.1"
                     /translation="GINAALAVSGQAPFVLERSQAYIGVMVDDLVTREILEPYRLLTS
                     RAEYRLLLRQDNADQRLTPLGYRLGLIGEERYRNTLERYEKIARETERLKETWLVPSM
                     EFNARLAELGVEPLSKMMSAGALICRPELEYHAVRTILGGSVDLDGDIGEQVATDLRY
                     EAYIQKQRAAVDRSRRLEQMAIPSDLDYSLIPGLRNEAREKLIRFRPATLGMASRISG
                     VTPADVSVLLIAIERRRRDRSVVASQDSPVVEPPLG"
     gene            813..2189
                     /locus_tag="VHS06_02165"
     CDS             813..2189
                     /locus_tag="VHS06_02165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2986958.1"
                     /translation="MKVSSSWLSPPHESSSSIDSVEAVELAPLMLVIGVAVAVRLIWL
                     DHVPTNVMPDEADNMADVYHILAGTGPSFIGFDWTQLPALSVYLMAGFVKLIGPSVLG
                     MRMAVLFTSTLALIPFYFLVRRLVSAIPALLATLMFSTGIWYLNFSRTAWANVHAVLF
                     GLVALWLLLEALDRRSMLLYGLTGGALALNLYGYYAGRATVLAILLFLPVGMLLHRAE
                     WRRVVSGYSLMLVVAIVLFLPQGLLIAQDWSYANTRVASVSIFQQSPPYLGQTDPVKL
                     LMLQSERIGRAFLLLDGSLFNTPRYTPAGSPPVDYVTGALYLAGLVLGTVLLRESLIW
                     YLLLLVPLFITQVLSILTPDTGRAIVVAPAIYLFVALSVQTLWSIGLRRGFAPAVVML
                     VVAIVAFNIWWYFDWVRSPFTAYVRQPAVELSEFGRWQQLQMERAARGERGFTVTEWH
                     QMRTQPSR"
BASE COUNT          389 a          695 c          736 g          534 t
ORIGIN      
        1 gggcattaat gccgcactgg cagtaagcgg tcaggcccca ttcgtgctgg aaagatctca
       61 ggcgtacatc ggggtcatgg tcgacgatct ggtgacgcgg gagatcctgg aaccttaccg
      121 actgctcacg tcccgggccg aatatcgcct gctcttgcgt caggataacg ccgatcaacg
      181 gttgacgccg ttggggtatc gcctcgggct aatcggtgag gagcgctacc ggaatactct
      241 ggagcggtac gagaagattg ccagggagac ggagaggctc aaggagacct ggctagtccc
      301 atccatggag ttcaacgccc ggttggcgga gttgggagtg gagcctctat ccaagatgat
      361 gtctgccggc gccctcatct gccgtccgga gttggagtac catgcagtaa ggacgattct
      421 ggggggaagt gtcgacctgg atggcgacat cggggagcag gtggccaccg acctgcgata
      481 cgaggcgtac attcagaagc agcgggcggc cgtggatcga tccagacggt tggagcagat
      541 ggccatcccc agcgatctgg actacagcct gatccctggt cttcgcaacg aggctcggga
      601 gaagctgatt cggttccggc cggcgacgtt gggaatggca tcgcggatct cgggcgtcac
      661 ccctgccgat gtgtctgtgt tgctcatcgc catcgagagg cgaaggcggg atcgatccgt
      721 tgtcgcctcg caagacagtc ctgtagttga accccctctg ggttgagcgg catcgtcggg
      781 accgttgcca ccgaatgagt tgaggagtga gggtgaaggt ctcaagcagt tggttgtccc
      841 cgccccacga gagcagttcc tccatcgaca gcgtggaggc tgtggaactc gcccctctca
      901 tgctggtcat cggcgtggcg gttgccgtcc gcctcatctg gctggaccac gtgcccacca
      961 acgtcatgcc ggatgaggcc gacaacatgg ccgacgtcta tcacattctg gccggcaccg
     1021 gacccagttt catcgggttc gattggactc aacttcccgc tctgagcgtc taccttatgg
     1081 cgggcttcgt caaactgatt ggcccatcgg tgctgggtat gcgaatggcc gttctgttca
     1141 ccagtacgct ggcgctgata cctttctatt tcctcgtgag gcggctggtg agtgccatcc
     1201 ccgccttgct ggccactctc atgttctcca ccggcatctg gtacttgaac ttctctcgta
     1261 ctgcgtgggc aaacgtgcat gccgtgttgt ttggccttgt tgcgctgtgg ctcttgctgg
     1321 aggcgctgga taggcggagc atgcttctgt atggcctgac tggtggcgcc ctggcgctga
     1381 acctgtacgg ttactacgcc ggccgagcga cggttttggc catcctcctc ttcctccccg
     1441 tcgggatgct gctgcatcga gcggagtggc gcagggtggt gtcgggatac agcttgatgc
     1501 tggtggtggc gatagtgctg ttcttacccc agggattgtt gatcgcccag gattggagct
     1561 acgcaaacac tcgcgttgcc tccgtatcca tctttcagca gtctccgccc tatctcggcc
     1621 agacggatcc ggtcaagttg ctgatgctgc agtccgagcg gatcgggcgc gccttcttgc
     1681 tgctggatgg cagcctcttc aatacgccca gatacacgcc ggccggcagc ccccccgtcg
     1741 actatgtcac cggggcactc tacctcgcgg ggctggtgct gggtactgtc cttcttcgtg
     1801 agagcttgat ctggtacttg ctgctgttgg tgccgctctt tatcacccag gtgctcagta
     1861 tcctcactcc cgacacgggc agggcaatcg tcgtggcacc tgcgatttat ctcttcgtgg
     1921 cactgtcggt ccaaaccctc tggtctatcg gcttgcgaag ggggttcgcc ccagctgtgg
     1981 tgatgcttgt ggtcgccatc gttgccttca acatatggtg gtacttcgat tgggttcgca
     2041 gccccttcac cgcgtacgtg cgccagcccg cggtcgagct ttctgagttt ggccgatggc
     2101 aacagttgca gatggagcgg gcggcccgcg gagagcgagg gttcaccgtc accgagtggc
     2161 atcagatgcg gacgcagcct tctcggtgat tccccggtcc ggccgcccac tagccaatac
     2221 tccttcttcc ctccaaacgg catgctttct gcattacccc cccacgcaac ggaccgcaag
     2281 ggcgcgggcg taggtcgagc ggatgcagtt tcggcaccac ctcggcagtg cgaggatacg
     2341 atagctcttg attt
//
LOCUS       DATALS010000101         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_234788, whole genome shotgun sequence.
ACCESSION   DATALS010000101 DATALS010000000
VERSION     DATALS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234788"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VHS06_02170"
     CDS             <1..584
                     /locus_tag="VHS06_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2986959.1"
                     /translation="MDLAREQPPDLVILDVMMPGIDGLEVCRRLRAADSALPIIMLTA
                     RDAVSDQVLGLQTGADDYVIKPFTFEVLLARIKALLRRTEANDTEQLRFADLVLDTGI
                     HTARRGGREIDLTTTEYDLLHEFMLHPRQVLAKDQILERVWGYHFEGNHNILEVYVRY
                     LRTKLEAGGGSRLIHTVRGAGYLLREPREGEED"
     gene            585..2066
                     /locus_tag="VHS06_02175"
     CDS             585..2066
                     /locus_tag="VHS06_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2986960.1"
                     /translation="MAVSSLRGRLAIWYATLLAIILAITSFLSYSFHSVYHYEEVDRA
                     LVGTAIHVLGKLRPDASTGGGGIVLPPIEEFDSPDIFVRVYAADGQIIAQSANSGHQA
                     LSSPQQVAASPRGVDSGGSISWLLHPLVSLGRPALAAEGGFLTTSGTDGRGRVRLYAV
                     PISNGSTVYSYLEAGVSLDSLDHSMERLRLLLATMSVVGLLAALGGGWAIAGSALRPM
                     ATMTTTARAIALSRGFSRRLPHLGRRDELGQLADTFNEMLASLEEAYQAQQRFIADAS
                     HELRAPLTAIQGNLELLERAPQMDESERAETLSYLRQEAGRMGRLVADLLVLARADAG
                     QPIRHQTVELDRVLLDVFQETRVLARGRKMAIVDLDQVQVLGDPDRLKQLLLILVDNA
                     IKYTPEGGRVQLAIRTEPGAAVITVADTGIGIGPEDIPHLFERFYRADKARSRDLGGT
                     GLGLSIAKWIADSHGGHISVESTVGQGSTFTIRLPLAHASREP"
BASE COUNT          399 a          629 c          668 g          406 t
ORIGIN      
        1 ggatggactt ggcccgagag caaccgccgg acctggtcat cctggacgtg atgatgccgg
       61 gcatcgatgg cctggaggtc tgtcgcaggc tccgagcggc cgactcagcg ttgccgatca
      121 tcatgctgac tgccagagac gcggtaagtg atcaggtgct tggcctgcag actggtgccg
      181 atgactatgt gatcaagccc ttcaccttcg aagtgctcct cgctcgaatc aaggctctct
      241 tgcggcgcac cgaggctaac gacactgagc agttgcgttt cgcagatctg gtgctggata
      301 ccggcatcca cacggcgagg agaggcggac gtgagatcga tctcaccaca accgagtacg
      361 atcttctcca cgagttcatg cttcatccca ggcaggtgtt ggccaaggat cagatactcg
      421 aacgtgtctg gggataccac ttcgagggaa accacaacat cctggaggtc tacgtccgct
      481 acctgcgcac caaattggag gccgggggcg gatcgcgctt gatacacacg gtccgcggtg
      541 ccgggtacct actccgggag ccacgagagg gtgaggagga ctagttggcg gtttcctccc
      601 tgcgtggacg cctggcgatc tggtatgcca ccttgctggc gattatcctg gcgattacct
      661 cattcctatc ctacagcttc cacagcgtct accactatga agaagtagac cgcgccctcg
      721 taggaaccgc tatccacgta cttggaaagc tcagaccaga tgcctccacc ggtggcggcg
      781 gtatcgtact gccgccgata gaggagttcg actcgccgga catcttcgtc agggtctacg
      841 cagccgatgg gcaaatcatc gctcagtcgg ccaactccgg ccatcaggcg ctgagcagcc
      901 cccaacaggt ggctgcctcg ccgagaggtg tggacagcgg aggatccatc tcctggttgc
      961 tgcatcccct ggtcagcctt ggccgtccgg cactcgctgc cgaagggggc ttcttgacca
     1021 ccagcggaac agatggccga ggcagggtaa gactgtatgc ggtcccgatc agcaatggca
     1081 gtaccgtcta ttcctatctg gaggccgggg tctcactcga ttcgctggac cactccatgg
     1141 agcgattgcg gctgttgctc gccacgatgt ctgtcgtcgg gttgctggca gcgcttggcg
     1201 gtggatgggc cattgccggc agcgcgctca ggccgatggc aacgatgacg accacggcca
     1261 gggcaatagc cctctccagg gggttctctc gccggttgcc gcacctcggg cgccgtgacg
     1321 agttggggca gttggccgat accttcaacg agatgctggc cagcctggaa gaggcttacc
     1381 aggcccaaca gcggttcatc gccgacgcgt cccacgaact cagagcgccc ctgacggcaa
     1441 tccagggaaa cctggagctt ctggagcggg ccccgcagat ggatgagtcc gagcgggccg
     1501 agaccctgtc ctacctgcga caggaggccg gtcgcatggg ccgtctggtg gcagacttgc
     1561 tggttctggc cagagcagat gcaggtcaac cgataaggca tcaaaccgtc gagcttgacc
     1621 gtgttctgtt ggatgtcttc caggagaccc gcgttctcgc tcgagggcga aagatggcga
     1681 tagtggacct ggaccaggtg caggttttgg gggatcccga ccgcctcaag caactgctgt
     1741 tgatcctggt ggacaatgcc atcaagtaca cgcccgaggg gggcagagta cagctggcaa
     1801 tacggaccga gcctggggcc gcagtgatta ctgtggcgga caccggcatc gggatcggcc
     1861 ctgaggatat tcctcatctg ttcgagcggt tctatcgcgc tgataaggct cgctctcgcg
     1921 acctgggagg taccggactg ggactgtcca tcgccaagtg gatcgcggac agtcacggtg
     1981 gacacatctc tgtggagagc acagttgggc aggggagcac cttcaccatt cggctgccgc
     2041 tggctcatgc ctcgcgagag ccctgatcct cgggccgtct ccggtcaacg cagagccgag
     2101 tc
//
LOCUS       DATALS010000102         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_189701, whole genome shotgun sequence.
ACCESSION   DATALS010000102 DATALS010000000
VERSION     DATALS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189701"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VHS06_02180"
     CDS             complement(<1..642)
                     /locus_tag="VHS06_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEX2986961.1"
                     /translation="MKAEIISVGTELLLGEIVDTNASYLSQKLADLGVDVHYRHTVGD
                     NLARLSDVISTALSRSDVVILTGGLGPTDDDLTREAIAEATGHPLRRVPASEQWLRDF
                     FAARNRPLADSNLKQADAPEGAEHLENCCGTAPGIYMQWQGKLIFAAPGPPTELREMA
                     ERSILPLLRRHLGETNQLYTRSLLMTDIGESQVADALRELIDAQVDPTIAMYAS"
     gene            943..>1609
                     /locus_tag="VHS06_02185"
     CDS             943..>1609
                     /locus_tag="VHS06_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HEX2986962.1"
                     /translation="MMTPSRVSPGLQTPFRYSLLALWPLLLLALALLGGCGGDEPPGG
                     ASAPSPASSRNVNTRRDRPVLLPVPRSLSWTGATFNIGATTDIVSWSAGDTVQQQAVA
                     RIQQAAVTAGLTPLVHRVGSRFAVGANHIVLRPPNTAERGLYPELTTKAEAYLLTVTS
                     YRVNVVALTSRGLFCAAQTLAQMLQSPSLDGCTIVDWPLQQFRAVHFTVNPGMYRDER
                     IYPA"
BASE COUNT          257 a          543 c          530 g          279 t
ORIGIN      
        1 cgaggcgtac atggcgatgg tggggtctac ctgggcgtca ataagctcgc ggagggcgtc
       61 agccacctgg gattccccga tatcggtcat cagcagggac cgggtgtaga gctggttggt
      121 ctcccccagg tgccggcgca gcaggggcag gatgctgcgc tcggccatct cccgcagttc
      181 cgtcgggggg ccgggggcgg cgaagatcag cttgccttgc cactgcatat agatgccggg
      241 ggcggtgccg cagcagttct ccaggtgctc ggcgccctct ggggcatcgg cctgcttgag
      301 gttgctatcg gccagggggc ggttgcgcgc ggcgaagaag tcccgcagcc actgctctga
      361 ggcggggacg cgccgcaagg ggtgaccggt ggcctcggcg atcgcctcac gagtgaggtc
      421 gtcgtcggtg gggccgagcc cgccggtgag gatgacaaca tccgaccgcg acagggcggt
      481 cgagatgacg tcgctaagcc gagcgagatt gtcccctacc gtatggcggt agtggacgtc
      541 aactcccaag tcagccagtt tctgcgacag ataggaagca ttggtatcaa caatctcgcc
      601 cagcagcagc tcggtgccaa ctgagatgat ttccgctttc aattgtgtaa agacttctgg
      661 ttagatgggg acgcgaagcg cgtgatagct gcctgaggcg accggcatcc aagttccccc
      721 gcgcgtgcgt gccggtggga tgcgcttgct cgacgcatac cggccgcctg aaacgaatca
      781 atgtaacagc agcttaaacc agattttagt gcaagagagg cgcttcgtca accgcgcctt
      841 ggcaccgcgg gagggccgtt gacgtagccg ttcccgggtg cgccggcgtc tcgccggcta
      901 tcggcggcag gataccgccc cggccgtgga gaagatgagc gcatgatgac accttcccgc
      961 gtttcgcccg gcctccagac ccccttccgc tactctctcc tcgccctgtg gcccctgctg
     1021 ctgttggcgc tggccctgct ggggggctgc ggcggcgatg agcccccggg cggcgcctcc
     1081 gcgccctccc ctgcgtcctc gcgcaacgtg aacacccgcc gcgaccggcc cgtgctcctg
     1141 ccggtcccgc gcagtctctc ctggaccggt gcgaccttca acatcggcgc caccacggac
     1201 atagtctcct ggtccgcggg agacactgtc cagcagcagg cggtcgcccg catccagcag
     1261 gcggcagtga ccgcgggtct cacgccgctg gtacaccggg tcggtagccg tttcgcagtg
     1321 ggggcaaacc acatcgtgct gcggcccccc aacacggctg agcgcgggct gtaccccgaa
     1381 ctcaccacta aggctgaggc ctacctgctg acggtcacct catatcgggt caatgtcgtg
     1441 gccctcacct cccgcggcct cttctgcgcc gcccagacgc tggcccagat gctccagagc
     1501 ccaagcctcg acggctgcac gatcgtggac tggccgctgc agcagttccg agcggtgcac
     1561 ttcacggtga accccggcat gtaccgcgac gagcgcatct atcccgccc
//
LOCUS       DATALS010000103         7921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_49457,
            whole genome shotgun sequence.
ACCESSION   DATALS010000103 DATALS010000000
VERSION     DATALS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49457"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..889
                     /gene="phoU"
                     /locus_tag="VHS06_02190"
     CDS             215..889
                     /gene="phoU"
                     /locus_tag="VHS06_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX2986963.1"
                     /translation="MAITTRATFDRQLAGLRDDVLILGSMVDKAIVRSVDALKRRDLN
                     ESEMIISDDMKINRKRFEIEEKCLLLLATQQPMASDLRVIAAAMHIITDLERMGDHAE
                     GIAKINLMMGDEPLLKPLVDVPKMAEKSRDMLQRALDAFITRNAEAATALAAEDDEVD
                     ALYNRVYSDLLQIMIADPTTISRATHLLWAAHNLERIADRITNICERIVFMVTGQMQE
                     MNVSKY"
     gene            complement(957..1874)
                     /locus_tag="VHS06_02195"
     CDS             complement(957..1874)
                     /locus_tag="VHS06_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2986964.1"
                     /translation="MISTAIDRVTVRRGLLLILLAGALWGTSGVTTKTLYRLSETTPI
                     SVAALRLALGAPVLLALSWRAVGGRYLKVRRSDFGLMLFAGASMGISQACYFSAIPRA
                     GVAAATLVAVCTAPVLVALLSAALLRERLTLVVGGALASALLGTVLLVDVGASQAGSQ
                     DYALGGILFALGAGLTSALLILTSRRLAGRCHPLQSIAIIVTVGALLLLAASSLTTGV
                     VLSYPPAGWALLIYLGLVPTALAYGLFFHGMRGATATVASIGALAEPLTSTLLAMLIF
                     GEQLGTLGLLGATLLIGTMLFLYRNDSAA"
     gene            complement(2325..3233)
                     /locus_tag="VHS06_02200"
     CDS             complement(2325..3233)
                     /locus_tag="VHS06_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2986965.1"
                     /translation="MGLWGGIEAGGTKFVCMIGRGPQDIHAEERFPTTTPRETLLHAA
                     TFFRQHSSPQTPLEGIGVASFGPVDLDPKSLTYGHTTSTPKPGWAGTNVVGMLVQELG
                     VPVAFDTDVNCAAYGEYKWGAARGLDSVIYITVGTGIGGGVVMNGKPVHGLVHPEIGH
                     MLIPHDRQSDPYPGWCPYHRDCLEGLAAGPSLKGRWGQPGNLLPEGHPAWELEAHYLG
                     LALANLILCHSPHRIVMGGGVMDQSFLLPLLRKKVQQSLNGYVQSPQILDEIDRYIVA
                     PVLGNKAGVLGAIALAQDLLARPTPV"
     gene            complement(3422..5266)
                     /locus_tag="VHS06_02205"
     CDS             complement(3422..5266)
                     /locus_tag="VHS06_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX2986966.1"
                     /translation="MQADPTISRKRRLPRARWVLLSVCVIAAALAISFVIRQQGAAKT
                     AAADQSSPVFSVATAKVAEGQITTSVTYAADVKATNQVSVVPKGTGRIERLAVDVGSR
                     VKKGDTIAELDADSLRAQVAQAKANLAAAKAKYSGMQGGPRDESVAQAKAALDSAQAR
                     LDTVKKGATESALQTAQGQLNTARANLQKAKAAQETVKLGPTQSEMAAAKAAVDAAKL
                     SIVNYQAALDDLKAGPKDAELWAAQKDVESARSNLYSINDKVTIWKGTSTDAEKMATG
                     YPSVSAAVEASGAAQTALDAAVARLNQLKAGATAAQLQDAQTKLMTAKSQYDSASAKL
                     EQLQRGSTPQDLQQADAGVQVAEAALATAEASLKQLQDGPTAEDVRVAEAAVAQAEQA
                     YNLAMKPYTQNDLGQAAASVQQAESAVELAEIGLREAVVKSPTDAVVAEKLQSEGALV
                     SPATPIVTLVSGDVELALSVEESKVGQLKEGQKAEINATAYPGEAILAAVSSISPTAD
                     AKSRTFQVKVRPTDQSGKLRPGMFAQVKIATSEKPKALTVPKDALVNRGGQTIVFVVN
                     GDTVQQRTVTTGIIQNGTAEITNGLSAGEEVVTAGQNDLKDGSQIKKG"
     gene            complement(5267..>7921)
                     /locus_tag="VHS06_02210"
     CDS             complement(5267..>7921)
                     /locus_tag="VHS06_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX2986967.1"
                     /translation="ERIDGVGTVYGFGGKVREIHVESSLEQLQSYGITPQQLSQAIRG
                     ENLDLPGGRITEGPTEEMLIRTAGKITSLEQLGDVPVTTPKGIVAHLKDLATITRSSA
                     EVRSYSRLDGADSVMVIVQKQGGANTVRVSETVQAGLKKLQKQYPGLSFGIIFDQSTF
                     TRDAINDVQLSLILGGILAALVVFLFFRDMRNTLVTVAGLPVVLLGTFIVMNAMGTSL
                     NMISMMALSLSVGLLIDDAIVVRENIFRHMEMGLEPKVAAGRGSAEIALAVLAVTSTI
                     VAVFLPIAFVGGIVGQFLRDFGLTVAVAVIISLVEAFTLAPMLSAIFFHRIDPETDRK
                     RKSSAFFRFFDGLNRAYGSLLAWALSHRWLVVAAGVVCLVCSLALVPLMNRTFEGTSD
                     QGEFAVMMELKPGMSLQEADRIARKAEQIVREEPEVATMFASVGSGMGAGGGANSASI
                     GAKLKSRGHTVQIVDGLRPKLAAALPGVKITYNTETAAAGAFGGAASAMTTQPIQFKI
                     QGADFDKLDQVSKDMVATLQTVPGIVDVDRSAKDGGPQRVIVLDRARATDLGISTNQL
                     ATTVRTLVNGDKAGTFQDVDKDIDIVVRLVDEQRDNTERILQLPISTTRGTQLPLSAV
                     ASLLPSTEPAKIDRENRQRQIIVAGGVSGADAGPAMAAAQKAVLSMKLPDGVTLVAGG
                     DVQQTNEAFAALIGALALSVAFMYMILASQFGSFIHPFTIMLALPFAIVGAMVALVMA
                     RFSLDAMSMIGMILLMGLVSKNSILLVEFTNQMRARGLSTREAILHAGPVRLRPILMT
                     TLSMICGMIPVAVGFGAGSEMRQPMGVSVIGGVITSTVLTLVVVPVAYSLIDDLGNWI
                     VRRFRRGKSSHSARVESRVAEAFVQGEN"
BASE COUNT         1555 a         2649 c         2301 g         1416 t
ORIGIN      
        1 catcaccggc cgcttcggct aagcggcttt ccctggatgc agtcgaccct tccatgagga
       61 gagagtggat gccaataagg ggaaggtcgc ttaggccagg cggacgaaag gtcgataaat
      121 ggacaaacag cccgacaaag aatacactcg gaagatagtg gatatggcag atgccagatc
      181 gttcggggga aagccccgag gaggtagtga agccatggcc atcaccactc gagctacctt
      241 cgaccgccag ctggcaggac tgcgcgacga cgtgctgatc ctgggcagca tggtggataa
      301 ggccatcgtc cgatccgtag atgccctgaa gcgacgcgat ctcaacgagt cggagatgat
      361 catctccgac gacatgaaga tcaaccgcaa gcggttcgag atcgaggaga aatgcctcct
      421 gctgctggca acgcagcagc cgatggccag cgacctgcgc gtgatcgccg ccgcaatgca
      481 catcatcacc gatctggagc ggatgggcga ccatgccgag gggatcgcca agatcaacct
      541 gatgatgggc gatgagccgc tgctgaagcc tctggtcgac gtcccgaaga tggcggagaa
      601 gtcgcgagat atgctgcagc gcgcgctgga cgccttcatc accaggaacg cggaggcagc
      661 gacggccctt gccgccgaag acgacgaggt ggatgccctc tacaaccgcg tctacagcga
      721 tcttctgcaa atcatgatcg ccgatccaac caccatcagc cgtgccaccc atctgctttg
      781 ggccgcccac aacctggagc gcatcgccga ccgcataacc aacatctgcg agcggatagt
      841 cttcatggta accggccaga tgcaggagat gaacgtctcc aagtactagg taggtagtag
      901 aaagaaagcg atagcgccat caggcgtctt ccgacgcctg atggcgctgc tgctttctat
      961 gctgcggagt cgttcctgta aaggaacagc atggtgccga tcagaagggt ggctccaagc
     1021 aacccgaggg ttcccagctg ctcgccgaaa atcagcatcg ccaacagcgt cgaggtcagc
     1081 ggctctgcca gcgcaccgat gcttgccacg gtggcagttg cgccacgcat gccgtggaag
     1141 aagagtccgt aggccagtgc tgtgggcacc agcccgaggt agatcagcag ggcccaacca
     1201 gccggcgggt agctgagcac caccccggtg gtcagactgg aggcagccaa cagcagcagt
     1261 gcacccaccg tgacaatgat ggcgatcgat tgaagtgggt gacaccggcc ggccaaacgg
     1321 cggcttgtca gtatgagcag tgctgaagtg agccctgccc ccagtgcaaa caagatcccc
     1381 ccgagggcat agtcctgcga accggcctga ctcgctccaa catcgacgag caacaccgtg
     1441 ccgagcagcg cgctggcaag cgccccgccc actaccaggg tcagccgctc cctgagcagg
     1501 gcggcagaca gaagcgccac gaggaccgga gcggtacaca ctgccaccag agtcgcagcc
     1561 gcgacgccgg cccgagggat ggccgagaag tagcacgcct gcgatatccc catcgaggcg
     1621 ccggcgaaca acatcaatcc gaagtcggag cgcctgacct tcaagtagcg gccaccaacc
     1681 gcacgccagc ttagggctaa cagcacgggc gcccccaggg ccagtcgaag cgcggcgaca
     1741 gagataggag ttgtctctga caatcggtag agtgtctttg tggtaacgcc ggaagtgccc
     1801 cagagggcac ccgcgagaag tatgagcagc agtccgcgcc gaaccgtgac gcggtctatc
     1861 gcagtggaaa tcattgcgaa cgatcctttc cccgataagc ttgatcggca atatgctgaa
     1921 ccagcctact gccggagagg gcaggatcgg cacaaccaac cgacggccca cctgcgaggg
     1981 tcgttgagag gagtacagcc gcctgcccct gctatgcgag ggcgggcggt ggtggggggt
     2041 taaaagcgta agctatcgct agcaatgtcg acttgtccta taggtcgaga taggtcggcc
     2101 ggctcaacca acgagagccg gtatccacct gggcacaata ttagcatggt ggatcagggc
     2161 tccacaacga ggccgagagt ccccacctcc cgtcgtacat gggctccgcc acggtactcc
     2221 tgatctccag gagcccggcg tgacgctagc gagcacgcga cggtagcgag cgtggcgtcg
     2281 cggtcacgct cgactcctag tgtcaagatc aacgtgtcag acgactagac tggagtaggt
     2341 ctggcaagga ggtcctgcgc cagcgcgatg gcgcccagca cccccgcttt gttccccaat
     2401 acgggagcca cgatgtaccg gtcgatctca tcgaggatct gtggcgactg cacgtacccg
     2461 ttcagcgact gctgcacctt cttcctcagc aacggcaaca gaaacgactg gtccatcacc
     2521 ccaccgccca tcacgatccg atgcggggag tggcagagga tcaggttcgc cagcgccagc
     2581 ccgaggtagt gcgcctccag ttcccacgcg gggtggccct cgggcagcag attccccggc
     2641 tgcccccatc tgcccttgag tgaagggccc gccgccaatc cctccaggca atcccggtga
     2701 taggggcacc agccgggata ggggtccgac tgtcggtcgt gggggatcaa catgtggccg
     2761 atctcgggat ggaccaaccc gtgcacaggc ttcccattca tgaccacccc gccgccgatg
     2821 ccggtgccga cggttatgta aatcaccgag tccagcccgc gcgcggcgcc ccacttgtac
     2881 tcgccgtagg cggcacagtt cacatcggtg tcgaaggcta ccggaacccc aagctcctgc
     2941 accagcatcc cgaccacgtt ggtcccggcc catcccggct tcggcgtgct ggtggtgtgt
     3001 ccgtaggtca gcgacttcgg atccaggtct accggtccga aggaagctac acctataccc
     3061 tccaaagggg tctgaggcga cgagtgctgc cggaagaacg tggccgcgtg gagcaaggtc
     3121 tctctgggtg tggttgtggg gaatcgctcc tccgcgtgga tgtcctgtgg gccgcgcccg
     3181 atcatgcaga cgaacttcgt acccccagcc tcgatgccgc cccacaaccc catcgccttc
     3241 actatcgatg tctcctctca acaccggcag tccggtaccg atccattcca ccacagcccc
     3301 aaaaggggag tcaagatcga tgcctgagcc aacgaagggt gtacaccgta cgacaagagc
     3361 cggcatggag ctctgtttgt ctccatgccg gctcttccct accgtaatcg tgagtccagg
     3421 actaaccctt cttgatctga gaaccgtcct tcaggtcgtt ctggccggcc gtcaccacct
     3481 cttcacccgc cgagagcccg ttggtaatct ccgcggtccc attctggatg atcccggtgg
     3541 tcactgtccg ctgctgcacc gtatccccat tgacgacgaa gacgattgtc tggccgccgc
     3601 ggttgaccag cgcatccttg ggcacggtaa gcgccttcgg cttctcggag gtggcgatct
     3661 tcacctgcgc gaacatcccc ggccgaagct tgccactctg atcggtggga cgcaccttca
     3721 cctggaaggt acggctcttg gcatctgcag tcggcgaaat cgacgagacg gcagccagaa
     3781 tcgcctcgcc aggataggca gtcgcgttga tctcggcctt ctgcccctcc ttcagctggc
     3841 ccaccttgga ctcctcgaca ctcagtgcca gctcgacgtc cccggacaca agcgtaacga
     3901 tgggcgtcgc ggggctaacc agcgcgccct ccgactgcag tttctccgcc acgacagcgt
     3961 cggtggggct cttgaccacg gcctcccgca gcccgatctc cgccaactcc acggccgact
     4021 cggcctgctg caccgatgcc gccgcctgcc cgaggtcgtt ctgggtgtag ggcttcatcg
     4081 ccaggttgta cgcctgctca gcctgggcta ccgcagcctc ggcaaccctc acatcctccg
     4141 ccgtcggccc atcctgcaac tgcttcagcg atgcctcggc agtcgccagc gccgcctcgg
     4201 caacctgcac gccggcgtcg gcctgctgca ggtcctgggg ggtagatcct cgctgcagct
     4261 gttcgagctt cgccgatgcc gagtcgtact gggacttggc cgtcatcagc ttcgtctgcg
     4321 catcctgaag ctgcgcagcc gtcgcacccg ccttcaactg gttcagtctc gccaccgcgg
     4381 catccagcgc agtctgcgct gcaccggaag cctcaacggc agcgctgacg ctcggatacc
     4441 ccgtcgccat cttctcggca tcggtgctgg tgcccttcca gatggtcacc ttatcgttga
     4501 tggaatagag gttggagcga gcagactcga catccttctg ggccgcccac agctcggcat
     4561 ccttgggccc ggctttgagg tcgtccaggg cggcctgata gttcactatg gaaagcttgg
     4621 ccgcgtccac cgccgccttc gccgccgcca tctccgactg ggtcggcccc agcttcacgg
     4681 tctcctgcgc cgccttagcc ttctgcagat tggccctggc agtgttcaac tgtccctggg
     4741 ccgtctgcaa cgccgattcg gtggccccct tcttcaccgt gtccagcctg gcctgcgccg
     4801 agtccagcgc cgccttcgcc tgggcaaccg actcgtctct cggaccgccc tgcatgccgg
     4861 agtacttcgc cttcgcagcg gccaggttcg ccttcgcctg ggcaacctga gccctgagcg
     4921 aatcggcgtc caactcggcg atggtgtcgc ccttcttgac ccggctcccc acatcgacgg
     4981 ccagcctctc gatccggccg gtacccttcg gcaccacgct cacctggttc gtcgccttca
     5041 cgtcggcggc gtaggtcacg ctggtcgtta tctgcccctc ggctaccttc gccgtcgcca
     5101 cggagaagac cggtgagctc tggtcggcag cggcggtctt ggcagccccc tgctgcctga
     5161 tcacaaagct gatggccagc gcggccgcga tgacgcagac cgacagtaat acccatctgg
     5221 ccctggggag tcttcgcttt ctgctgatag tgggatcggc ctgcattcag ttctctcctt
     5281 gcacaaaagc ctcggctact cggctctcaa ctctagcact atgactactc ttccctctcc
     5341 ggaagcggcg aacgatccag ttgcccagat catcgatcag cgaataggcc acagggacca
     5401 ctacaagtgt gagcaccgtg gaggtgatga cgccgccgat cacgctcacg cccatgggct
     5461 ggcgcatctc agatcctgca ccgaagccaa cggcgacggg gatcatgccg cagatcatgg
     5521 agagggtcgt catcaagatc ggccgcaacc tcaccggtcc tgcgtggagt atcgcctcgc
     5581 gagtgctcag cccccgtgct ctcatctggt tggtgaactc caccagcaag atggagttct
     5641 tgctcaccag gcccatcagc aagatcatgc ctatcatgga catggcgtcc agactgaagc
     5701 gggccatcac cagcgccacc atagcgccga caatcgcaaa tggcaacgcc agcataatgg
     5761 tgaacgggtg tatgaagctg ccaaactggg aggccaggat catgtacatg aaggccaccg
     5821 agagggccag tgccccaatc aaagccgcga aggcctcgtt ggtctgttgc acatctcctc
     5881 cggccaccag cgtcacccca tcaggaagct tcatcgacaa cactgctttc tgtgccgccg
     5941 ccattgccgg accggcatca gcacccgaaa cgccacccgc cacgattatc tggcgctggc
     6001 ggttctcgcg atcgatctta gcaggttcag tcgatgggag aaggctggcc acggcggaga
     6061 ggggtagctg cgttccgcga gtcgtagaga tgggaagttg caggatccgc tccgtgttgt
     6121 cccgctgttc gtccaccagc ctgaccacga tatcgatgtc cttgtccaca tcctggaacg
     6181 tccctgcctt gtccccatta accagcgtcc gaacggttgt cgcgagctgg ttagtggaga
     6241 tcccgagatc ggtagctcgg gcacgatcca gcacgatcac cctctgcggg ccgccatcct
     6301 tcgcagaacg atccacatcg acgatcccag gcaccgtttg aagcgtcgcg accatatctt
     6361 tagagacctg gtccaacttg tcgaaatcgg caccctggat cttgaactgg atcggctgag
     6421 tggtcatagc cgaggcggca ccaccaaagg cgcctgccgc cgcggtttca gtattgtagg
     6481 tgatcttgac cccgggtaaa gcagccgcga gctttggccg aaggccatcc acgatttgga
     6541 cggtgtgtcc tcggctcttc agcttagcgc cgatggaagc cgagttcgcc ccaccgccgg
     6601 cgcccatgcc ggagcccacg ctggcgaaca tcgtcgcaac ctcgggctcc tccctcacga
     6661 tctgctccgc cttccgcgct atcctgtccg cttcctgaag gctcatacct ggcttcagct
     6721 ccatcatcac cgcgaactcg ccctggtcgg aggtcccctc gaaggtgcgg ttcatcagag
     6781 gcaccagtgc caggctgcat accagacaca cgacaccggc ggcaaccacg agccagcggt
     6841 gactgagcgc ccacgccagc aagctcccat atgccctgtt gaggccatcg aagaaccgga
     6901 agaacgcact cgacttcctc ttccgatccg tctcggggtc gatcctatgg aagaaaatgg
     6961 ccgagagcat cggggccagg gtgaacgcct cgaccagcga gattatcacc gccaccgcga
     7021 ccgtcaagcc aaagtcgcgc aggaactgcc ccacgatgcc gccgacgaag gcaatgggca
     7081 agaacaccgc gacgatggtc gaggtgacgg caagcacggc cagggcgatc tccgcggacc
     7141 ctctgccggc cgcaaccttc ggctccagcc ccatctccat gtgccggaat atgttctcgc
     7201 gcaccacgat ggcatcgtcg atcagcaacc ccaccgagag ggacagggcc atcatggaga
     7261 tcatgttcag cgaggtaccc atagcattca tcacgatgaa ggtgccgagc agcaccaccg
     7321 gtaaacccgc caccgtgacg agcgtgtttc gcatatctcg gaagaacagg aataccacca
     7381 gggcggccag gatgccaccc aggatcaacg acagttggac gtcgttgatg gcgtcccgag
     7441 tgaaggtcga ctggtcgaat atgatcccga agctcagccc gggatactgc ttctggagct
     7501 tcttcagccc cgcctgaacc gtctcggaaa cccgcaccgt gttggcccca ccctgcttct
     7561 ggacgatcac catgacgcta tcggcaccgt ccagacgtga gtaggagcga acctcagcac
     7621 tggatcgggt aatcgtcgcc agatccttga gatgagcgac gatccccttc ggggtggtca
     7681 ccggcacgtc ccccagttgc tcgagggagg tgatcttgcc cgccgtccgg atcaacatct
     7741 cctccgtagg tccctcggtg atccgcccgc cgggcaggtc caggttctca cccctgatcg
     7801 cctggctcaa ctgctgcggg gtgatcccgt aggactgcaa ctgctccagg ctcgactcga
     7861 cgtggatctc cctcaccttc ccgccaaatc catacacggt ccccacgccg tcgatccgct
     7921 c
//
LOCUS       DATALS010000104         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_94740,
            whole genome shotgun sequence.
ACCESSION   DATALS010000104 DATALS010000000
VERSION     DATALS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94740"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VHS06_02215"
     CDS             <1..452
                     /locus_tag="VHS06_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX2986968.1"
                     /translation="DAMVHAEKQGADTLVDLATLTGGCVVALGTGGAGLMGNDDALMQ
                     ELTEAGNRSGERLWQLPMWEEYLWEQVRSDFADIKNSGGRYASAQTGAIFIEQFVDKA
                     KWAHLDIAGTAWIGSDQPSLPKSYLPKGPTGFGVRLLLHFITKRAEG"
     gene            493..2019
                     /locus_tag="VHS06_02220"
     CDS             493..2019
                     /locus_tag="VHS06_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX2986969.1"
                     /translation="MAASVLVMALAAAALLRFRPGDEGSALHKVAKNSLFPMATSFLN
                     KFLDLGFAVYMFRVLGPEGVGAYALAQVVLGYLDIVANFGLGTLLTREVAKDRLSVNR
                     FLGNTLAVRVGLWLLCLPFLALLIGPGAGLMGITPEVALAIVLLVAGLLPSLVAGTVS
                     ALFQAYERFEYPAIVTVLTNILKIGLGVVALSSGWGFVGLAGVSVVVNLITVGMLFAL
                     MLALLVRPRWEYNAGFSWKLLGVSYPLMLNNLLNSVFYRVDSLMLTPMAGSNALGFYS
                     TAYKFIDGLGIISSTFTMALFPLLSQYAHGARNSLARAYGLAIKLLVMASLPICVGTT
                     LIAPQIILLFAGEQYLPHSAVALQILIWFLPFSFVNGVTQYLLIAINQQRFITLSFVV
                     AALFNVGANLLLIPALGYVGAALTTVASELVLMVPFWYCVRRHVPPIPVLSLAWRPVV
                     ASAAMGLEVWLLQDWNLIAAIVLAVPIYGAGLLALGSFDKSERAMIGSMLPRRLRTTS
                     "
     gene            2214..3938
                     /locus_tag="VHS06_02225"
     CDS             2214..3938
                     /locus_tag="VHS06_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HEX2986970.1"
                     /translation="MRNSLEQRLRSIDRRGYPAYKELAGAHEFPNYTLYIDHVQGDPF
                     AAPSMLRVRVPQQVADFPATLYSSPVRRVALEDYLTRQFAAAIRRHVKGSRGTGKSGR
                     ISVDVGGQEILERTSCVVNGRFVEIRFSAGLPAAGRTVLAGEAIAMFYQEIPRLVEAS
                     LRYSALNPQSVQTHVEVAEDDRALREQLEGMGLVAFVADGAILPRESGVSARPMAADR
                     AVPFASPPQLQVELQAPNRGKIPGMGIPAGITLIVGGGYHGKSTLLSALQMGVYDHIP
                     GDGREYVVTRTDAVKIRAEDGRRVERVNVSPFISNLPFQKDTTAFSTDNASGSTSQAA
                     NIVEALEAGSRLLLMDEDSCATNFMIRDEKMQKLVPKEREPITPLIDQVRSLFDEHGV
                     STLLVMGGSGDYFEVADTVISLDSYRPKVVTDRARQIASEDPSPRRQEAAGGFGRIVE
                     RIPLAESLNPYRGERVKVRARGMENIQFGDENIDLDDVEQLVDPSQSVAIANMLLYAL
                     NRGYLDNRTTLRRLLERIYGDLASGGLDAISPFRGERHPGDYAMPRMQEVAAAINRLR
                     TLAVRQVP"
BASE COUNT          858 a         1480 c         1565 g          866 t
ORIGIN      
        1 ccgacgcgat ggtccacgcc gagaagcagg gcgcggacac cctggtggac ctggctacgc
       61 tgaccggggg gtgcgtggtg gcgctcggga ccggcggtgc cgggctgatg ggcaacgacg
      121 acgccctgat gcaggaactg accgaggccg gcaatcgatc gggcgagcgg ctgtggcaac
      181 tcccgatgtg ggaggagtac ctctgggagc aggtgcgcag cgatttcgcc gacatcaaga
      241 acagcggcgg gaggtacgcc tcagcgcaga cgggggcgat cttcatcgag cagttcgtcg
      301 acaaggctaa gtgggcccac ctggacatcg cgggcacggc ctggataggc agcgaccagc
      361 cgtcactgcc caagtcctac ctgcccaagg gaccgacggg cttcggcgta aggctgctgc
      421 tccacttcat tacgaaacga gcggagggat aagatccagc tttcctcgag acagcaacta
      481 ttcgctatcc tggtggcggc cagcgtcctg gtcatggcgc tggctgccgc cgccctgctg
      541 cgatttcgcc ccggcgacga gggatccgcg ctccacaagg tggccaagaa ctccctgttc
      601 cccatggcca cctccttcct gaacaagttc ctcgacctgg gcttcgccgt ctatatgttc
      661 agggtgctgg gcccggaggg ggtcggcgca tacgcccttg cccaggtggt cctgggctac
      721 ctggacatcg tggccaattt tgggcttggc acgctgctga cccgggaggt cgcgaaggac
      781 cggctgagcg tcaaccgttt cctggggaac accctcgctg tgcgagtggg gctgtggctg
      841 ctttgcctgc ccttcctggc gctgctgatc ggccccggcg cggggctcat gggcatcacg
      901 cccgaggtcg ccctggccat agtgctgctg gttgcggggt tgctaccgtc gctggtggcg
      961 ggcaccgtca gcgccctctt ccaggcatac gagcgcttcg agtacccggc catcgtcacc
     1021 gtgctcacga acatacttaa gatcggactg ggggtggtgg cgctctcctc cggttggggc
     1081 ttcgtggggc tggccggggt ctccgtggtg gtcaacctga tcactgtggg aatgttgttc
     1141 gccctgatgc tggcgctgct ggtaaggccg cgctgggagt acaacgctgg gttctcctgg
     1201 aagctgcttg gggtctccta cccgctgatg ctgaacaacc tgctcaactc ggtgttctac
     1261 cgggtggaca gcctgatgct gacgcccatg gcgggcagca acgccctcgg cttctacagc
     1321 acggcctaca agttcatcga cgggctgggg atcatctcct ccactttcac catggccctc
     1381 ttcccgctgc tctcccaata cgcccacggc gcccgcaact ccctggccag ggcctacggc
     1441 ctggccatca agctgctggt gatggcgagc ctgccgatct gcgtgggaac aaccctgatc
     1501 gccccacaga tcatcctgct gttcgccggg gagcagtact taccccactc ggccgtggcg
     1561 ctgcagatcc tgatctggtt cctgcccttc agcttcgtca atggggtcac ccagtacctg
     1621 ctgatcgcca tcaaccagca acgcttcatc acgctcagct tcgtggtcgc cgcgctcttc
     1681 aacgtcgggg caaacctcct gttgatcccc gccctgggct acgtcggggc ggccctcacc
     1741 acggtggcct cagagttggt gttgatggtg ccgttctggt actgtgtgcg gcggcacgtc
     1801 ccgcccatcc cggtgctctc cctggcctgg cgtccggtcg tggcctctgc ggccatgggg
     1861 ctggaggtgt ggctgctgca ggactggaac ctgatcgcgg cgatagtgct ggcagtgcct
     1921 atctacggtg cggggctact ggcactcggg agcttcgaca agagcgagcg ggcgatgatc
     1981 ggcagcatgt tgccgcgaag gctcagaaca acctcgtagt ggcgacacac tcgtcacctg
     2041 acgtgcggag gcccacaaag accaaacgaa caggatagac aggatataca ggatggattc
     2101 agagtctgtg atccatatcc tgtgcatcct gtctatcctg tttgaatcgt cccgaacgcg
     2161 cggtcgcatc gcggtcatgc cggcgagacg cttgcgctcc gaaaggacac atcatgcgaa
     2221 acagcctgga gcaacggctc cggtccatcg atcggcgagg ctaccccgcc tacaaggagc
     2281 tggccggcgc ccacgagttc cccaactaca ccctctacat cgatcacgtt cagggagacc
     2341 ccttcgcggc accctccatg ctgcgggtca gggttcccca gcaggttgca gacttccccg
     2401 cgaccctcta ctcctcgccc gtccggcgcg tcgcgctgga ggactacctc accaggcagt
     2461 tcgccgcagc gatacgtcgg cacgtcaagg gcagccgggg caccggcaag tccggccgga
     2521 tatcggtgga cgtagggggc caggagatcc tggagcgcac ctcctgcgtg gtgaacggac
     2581 ggttcgtgga gatccgcttc tcggcggggc tgccggcagc cgggcgaacg gtgttggccg
     2641 gagaggcgat cgcgatgttc taccaggaga tccctcggct ggtggaggca tcgctacgct
     2701 actccgccct gaaccctcag agcgttcaga cacacgtgga ggtggcagag gacgaccggg
     2761 cgttgcgtga gcaactggag ggaatggggc tggtggcctt cgttgccgac ggagcgatcc
     2821 tccccaggga gagcggggtg agtgcaaggc cgatggcggc ggatcgcgca gtgccattcg
     2881 cgtcgccgcc tcaactgcaa gtggagttgc aggcgcccaa ccgcggcaag atccccggga
     2941 tggggattcc ggcaggcatc acgctgatcg tagggggtgg ctaccacggc aagtcgacgc
     3001 tgctctccgc cctacagatg ggggtgtacg accacatccc gggggacggt agggagtacg
     3061 tggtaaccag gaccgacgcg gtgaagatcc gggcggagga tggccggcgg gtggaacggg
     3121 tcaacgtgtc gcctttcatc tccaacctac ccttccagaa ggacacgacg gccttctcca
     3181 cggacaacgc cagcggcagc acctcccagg cggcgaacat cgtggaggcg ctggaggcgg
     3241 gcagccggct gctgctgatg gacgaggaca gctgcgccac caactttatg atccgcgacg
     3301 agaagatgca gaagctggtg cccaaggaga gggagccgat cacccctctc atcgaccagg
     3361 tgcgcagtct gttcgacgag catggggtat ccacgctatt ggtgatgggc ggctccgggg
     3421 actatttcga ggttgccgac accgtgatct ctttggatag ctaccggccg aaggtggtga
     3481 ccgatcgggc acggcagatc gcctcggagg atcccagccc cagacggcaa gaggcagccg
     3541 gcggcttcgg caggatcgtg gagcggatcc cgctggcgga aagcctcaac ccctaccgag
     3601 gtgagcgggt gaaggttcgg gcccgcggca tggagaacat ccagttcggg gatgagaaca
     3661 tcgacctgga cgacgtggag caactggtgg acccgtccca gagcgtggcc atcgccaaca
     3721 tgctgctgta cgcgctgaat cgggggtacc tggacaatcg cacgacgctt cgacggctgc
     3781 tggagaggat ctacggggat ctggctagtg gcgggctgga cgccatctcg cccttccgcg
     3841 gggaacgcca ccccggggac tacgccatgc cgcgaatgca agaggtggca gcggcgatca
     3901 accggctgcg aacgctggcg gtgaggcagg tgccgtagtg ctgagtgctg agtgctgagt
     3961 gctgagtgct gagtgctgag tgctgagtgc tgagtgctga gtgctgagtg ctgagtgctg
     4021 agtgctgagt gctgagtgct gagtgctgaa aggtattgta ccagcgtgat cgagacgtag
     4081 ggcggggcac aagaccccgc ccaaagcgtg gtctgaatca ccttgatggt cgtggccgcg
     4141 gtgttggagg cggggcacaa gaccccgccc tacgagacgc cgcaggtggc ttccaccgag
     4201 caaacggtca tccgccagac ttacaccacc tgaatcgtat tggccaactc gcacacaaga
     4261 ccgtgatgcg accacgcgtg ggggacagat tcgaacagga tggacaggat atcccggata
     4321 tacaggatga gaatcgaaat gattcgtcct catcctgcat atccgtctca tcctgtccat
     4381 cctgttcgct taccttctgc gggcctttgg cctgcccgta ggcaagaacg cccgcgtgcc
     4441 caaggctcat cacgccgtcg cacttcggaa cggtgtatta gtccctgtcc ccactcggca
     4501 caaagggctg ggaggctgaa tacaaaaatg gggggatgcg attgatcgca tcccccacag
     4561 taaaccgcaa cttgagccgg gccgcttctt cagtcgactg ccgtcacccc agctgctgcc
     4621 gctccagagc cagccgatcc tcgttgccgc aatggcggac caagaagcaa gcccctgaga
     4681 gctcgtccag atccttggca gcgatgatca acaccgactt gccctgataa ctcacaacct
     4741 tgtccccctc gctgggaaag ctgagggac
//
LOCUS       DATALS010000105         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_9879,
            whole genome shotgun sequence.
ACCESSION   DATALS010000105 DATALS010000000
VERSION     DATALS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9879"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..593
                     /locus_tag="VHS06_02230"
     CDS             48..593
                     /locus_tag="VHS06_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2986971.1"
                     /translation="MDAISGISTTSATATQQAHRGKGPKEFIEQLANKLGIDASKVEE
                     AFQQARSEGEQGENVLSSVADKLGIDVEKLEEAMQDIRRAGPPDRQQMLEKVASKVGV
                     DSSKLIEAFQQASSEDDGEDDLLSSVADKLGIDVDTLEQAFQQVRQESRPPAGGPPPS
                     SGDQSRPPDGWGEDGSFDIKA"
     gene            complement(854..>2356)
                     /locus_tag="VHS06_02235"
     CDS             complement(854..>2356)
                     /locus_tag="VHS06_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2986972.1"
                     /translation="DLDSQASVILAERLASPNRVRAVYYFDSTSGRLLVNVNDPLESL
                     LSLKSAAGIVSRPPAQPGDEVVATYRMADTIGTHLSEVSFSGLDRIPVFYVGTRLTFA
                     DQASRVVVVEVDLRDVWQRIGLSTVGQTGFAFAVSREGVLIAHPDPALLGTPMPQELE
                     PLTRGFEGSSTYSDPARRRPIFAAYSPVGGSTGWGIVVQQDKAELNAGVLSMSTWVIG
                     ISLALAFFGTLGILILVRNLTKPIVELTKITQGIAQTGRLTKTVMEHQSDEIGQLSQA
                     FDQMIERLQTTEGRVATAAAEERTRLARDLHDAVTQTLFAASLIADVLPVLWKRKPEE
                     GLTRLAELRELTRGALAEMRILLLELRPAALEEAQIDYLLRQLAESITGRSRVPITVM
                     VEGQASLPVEVKIALYRIAQESLNNMAKHAQAKHAIVNLHCEPERVVLRVTDDGKGFD
                     TARTRPDSLGLGIMRERAKGIGARLSIESEIGQGTSIEAIWGKDSDEGEL"
BASE COUNT          466 a          708 c          752 g          430 t
ORIGIN      
        1 cctcttcgca aacatggtta cagcaagaaa ggagaaagaa gtagacagtg gatgcgattt
       61 cgggcataag taccacaagc gcaacggcga cgcagcaagc ccatcggggg aaggggccga
      121 aggagttcat cgagcaattg gcgaacaagc tcggcattga tgcgtcgaag gtagaagagg
      181 cgtttcagca ggccaggagc gagggtgagc aaggggaaaa cgttctcagt tccgtggcag
      241 acaagctggg catcgacgtg gagaagctgg aggaggccat gcaggatatc cgccgcgccg
      301 gcccaccaga ccggcagcag atgctggaaa aggtggcgag caaagtcggc gttgactcct
      361 ccaagcttat cgaagcgttc cagcaggcta gcagtgagga cgacggagag gatgatctcc
      421 tctcctcagt ggcagacaag ctgggcatcg atgtcgacac gctggagcag gcgttccagc
      481 aggtgcgaca ggaatcccgg ccgccggcag gtggcccgcc gccatcgagc ggggaccagt
      541 ctcgtcctcc agatgggtgg ggagaggacg gctcgttcga tatcaaggcc taacctgacg
      601 agagcgtgcg tgaaggcgca ttgctctgtc tgattgggga acccgagtcc tgtcatgtgg
      661 tggcacaatc cgggcaattc tcgttgaggt tgtggtggtg agacagcggt gggcgcgccg
      721 gatgccgccc accttctttc cagtgacatt cccccctccc tgggagaaag agagtgatat
      781 ctcgcggcat cccagcgagg atgccgcgag atatcatccc ctgttcgggg ggattatgct
      841 gtatcggtcg cgtctagagt tccccttcgt ccgagtcctt accccagatt gcctcaatgc
      901 tggtgccctg gccgatctcg ctttcgatcg acagccgcgc gccaatgccc ttcgcgcgct
      961 cgcgcatgat gcccaatcca aggctgtccg gccgagtcct tgccgtgtcg aagcccttcc
     1021 cgtcgtccgt caccctgaga accacccgct ccggctcaca atgcagattc acaatcgcgt
     1081 gtttcgcttg agcgtgcttg gccatgttgt tgagcgactc ctgggcgatg cggtacagcg
     1141 cgatcttgac ttcgaccggc agcgaagcct gcccctccac catgacggtg atcggcacac
     1201 gcgagcgccc ggtgatcgat tcggccagtt ggcgcagcag atagtcgatc tgtgcttcct
     1261 cgagggcggc gggtctcagc tcgagcagca agatgcgcat ctcagcgagg gcgccgcgcg
     1321 tgagctcccg caactccgcg aggcgtgtca gcccctcctc cggcttacgc ttccagagga
     1381 cgggcagcac atcggcaatg aggctggcag cgaacagcgt ctgagtgacg gcatcgtgca
     1441 ggtcccgggc gagccgggtg cgctcttcgg ccgcggcggt cgccaccctc ccttccgtcg
     1501 tctgcaagcg ctctatcatc tggtcaaatg cctggcttaa ctggccaatc tcgtcgctct
     1561 ggtgctccat gacggtcttg gtgagacggc cggtctgggc aatgccctgt gtgatcttag
     1621 tcagttccac gattggcttg gtgagattcc tgaccaggat caggatgccc aaggtgccaa
     1681 agaaggccag cgcgagcgag ataccaatga cccaggtgct catgctcagc acgccggcgt
     1741 tcagctcggc cttatcctgc tggaccacga ttccccagcc ggtcgagcca cccacgggac
     1801 tgtatgcggc gaaaatcggg cggcgcctgg cggggtccga gtacgtagaa gagccctcga
     1861 acccccttgt caggggctcc agctcctgcg gcatgggggt tccaaggaga gccgggtccg
     1921 ggtgagcaat cagaacgcct tccctggata ccgcgaaggc aaaccccgtc tgcccgaccg
     1981 tgctcaggcc gatacgctgc cagacgtctc gcaggtccac ctcgacgacc accactcggg
     2041 acgcctgatc cgcgaatgtc agacgcgtgc cgacgtagaa gaccggaatg cgatcaagcc
     2101 cgctgaagct gacctccgaa aggtgagtac cgatggtgtc tgccatccga taggtagcga
     2161 cgacctcatc gccaggttgg gcgggggggc gagagacaat tccggctgcg cttttcaacg
     2221 acaggagact ctccagggga tcattgacgt tcactagcag gcggccgctg gtgctatcga
     2281 agtagtagac ggcgcgaact ctgttcggtg aggcaaggcg ctccgccaga ataacagacg
     2341 cctggctgtc aaggtc
//
LOCUS       DATALS010000106         6783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_235158, whole genome shotgun sequence.
ACCESSION   DATALS010000106 DATALS010000000
VERSION     DATALS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235158"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VHS06_02240"
     CDS             <1..676
                     /locus_tag="VHS06_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX2986973.1"
                     /translation="GMGESMVEEKLKELTYSENPTLATYAKEDGIHVRISAKAENTVA
                     AEAMVADFESKARSILGDLIYGVDDQSVPEAVMSLLDRCGLTLGTMEAPTAGQLSSSL
                     AGGDPEGRRFLGGMVAYNRSLLETMGVDIALISDHGIVSEEVARAMATVARHTVHSDV
                     GLSVVCGMEDGAEGGQRLGLVFCAVDDRGDVSASTTRYTTTWREVQRRSILDAIDLLR
                     RRILAK"
     gene            871..943
                     /locus_tag="VHS06_02245"
     tRNA            871..943
                     /locus_tag="VHS06_02245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:904..906,aa:Arg,seq:tct)
     gene            1175..2830
                     /locus_tag="VHS06_02250"
     CDS             1175..2830
                     /locus_tag="VHS06_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2986974.1"
                     /translation="MTVYRSSGRTELLGLGDSALERVARIAGSTLDREELLQALFVEL
                     NRSIPCDSLGFYWREPGESFCLVIVRPGLTAIEVEQTQVVGEAPGADDKPQPYCCQEN
                     SSSTHPVLRWLAAHDLSSSLNVPVVVAQQLLGYFVANRTGSHAFAVADLHPLVQIAGM
                     VGPSLNSMQLYERLELAHQNLKATRDELARSERLMVQGELASGVAHDINNILGLISAR
                     TDLLKLQGLPPQAAASAEAISQAVDDGITVVRRMTQFTRRQRKQEVVPLDVNKLVDDV
                     MEMTRPRWQSASSLNKVSVRFTPGPISTVLGVPSELREVMVNLVMNAVDAMPSGGWIR
                     IDVEQADGHAYISVADTGCGMSEEVREHIFDPFFTTKGESGSGLGLWVSHGIVVRHGG
                     DIEVHSEVGKGSRFRVRLPLARAVQEQAPKRVECVEYSILVVDDEAGLGEALRLSLEM
                     AGYHVLFSANPRQALEIFKQEQFDLVITDLRMPGMSGWELASEIKRFNPATPIIAMTG
                     WPVDLIRDGQRCSEMEAIIQKPYRVNDLRAKVARILAERKEKP"
     gene            complement(2892..4097)
                     /locus_tag="VHS06_02255"
     CDS             complement(2892..4097)
                     /locus_tag="VHS06_02255"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX2986975.1"
                     /translation="METARLIAVAITAGTLGILLRSTRPLRQAGQAEVTDSQSLVKAA
                     LHRVNIALEDRLDQRTILELIARSALEITSGWACRLSLLDGPTLVVGQAGVYWREADD
                     DAPSELPPAMAASLSESITARMTGLGDELGTLEVWAWRNMPFAPAQEESLSYFASEVA
                     AAVKNVMMVTATLSHASAVESKILEMSLWQRQLNEYCDDLARTHEELTQTRSVLEETY
                     MATLEALAGAVDARDPYTYGHCKRVAEIASSLALQMGLPDMDRSSLELAALLHDIGKI
                     GVPDNVLRKVTPLSQEDAAAIHQHPEMGYRMLSALRFLGGGLAAVRYHHERFDGSGYP
                     YALSGDQIPLLARVLAVADAFDAITSDRPYRRARSRDQGVEEVRRCAGSQFDPSVVEA
                     FVAFMNTAA"
     gene            complement(4356..5507)
                     /locus_tag="VHS06_02260"
     CDS             complement(4356..5507)
                     /locus_tag="VHS06_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2986976.1"
                     /translation="MVGSQRSLDEEEAAVSREMISLASEILSIDEACQTVLTLFARIF
                     PCVGSWMATTRTPDGAPQLRATSGFSWIGEADLSALSPQRLTEIHSARTGLWVIDIEH
                     FPTALPFVDEDVGAVVIAKLSAHNCCVGIVTLMLPTQWSFTPHQRQLLASLATQAALV
                     VHNANLVAAAQELVIQEERSRIATEIHDGVSQNLALLMLKVEIISRLIDMDPQRAKAE
                     LEKLQSIVELSVQELRRSINTLRSPDSAGLGLMPAIQRVVTDFTRQTGMEVELALPSE
                     VSLPPQAISTILGVIQERLATVSKQASASRVAVEVETACHLLTMRVADDGHTSLHPPF
                     DENAATPPLLDKLRDLVRPIGGTVSTTADLTGMVVESRIPLRQCMTPSH"
     gene            5691..6356
                     /locus_tag="VHS06_02265"
     CDS             5691..6356
                     /locus_tag="VHS06_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2986977.1"
                     /translation="MKKARVMVADDHTLFREGLRQLLEMERDIEVVGEASDGMEAVAK
                     ARTLVPDVILMDINMPVVGGVAAIGQILSERPETGIIVLTMHRQDQYVFDAMRAGARG
                     YLLKDAKPADLIFAIRMVARGASLVDPRMTTTVLKEFRRLASQVEPDQGVAGLTPKEV
                     EILKLLAMGLSNKEIGRELCLAEKTVKNYLSTVFQKLQINDRVQAAIYALKHGLLTEQ
                     EIS"
BASE COUNT         1322 a         1862 c         2158 g         1441 t
ORIGIN      
        1 cggcatggga gagagcatgg tagaggaaaa gttgaaggaa ctgacctact ccgagaaccc
       61 cacgctggct acctatgcca aagaggacgg gatacacgtt cggatcagcg cgaaggctga
      121 gaacactgtg gctgccgagg caatggtcgc agatttcgag tcgaaggcca ggtcgatcct
      181 gggggacctg atctacgggg tggacgatca gagcgtgccc gaggctgtga tgtcgctgct
      241 ggatcgctgc ggattgacac tggggacgat ggaagcaccg actgcggggc agctgtcgtc
      301 atcgctggcc gggggagatc cggagggtcg acgcttcctg gggggtatgg tcgcctacaa
      361 ccggagtctc ctggaaacga tgggcgtcga cattgcgctg atctccgatc atggcatcgt
      421 gagcgaggag gtagccagag ccatggctac ggtagctcgc catacggtgc actccgatgt
      481 cggcctttct gtggtgtgtg gcatggagga cggagcggag ggcggtcagc ggctgggtct
      541 ggtgttctgc gcggtggatg atcgcggcga tgtgtctgct tccaccaccc gatacacgac
      601 cacgtggcgg gaagtccagc gacgctcgat cctggatgca atcgatctcc tacggcgacg
      661 gattctggcg aagtagcctc gctgccacgt gtttagcatg gcttgcctgc ctggtgggcc
      721 gaccagttag gcctaccagg cgaatgggca ttcgcaaaga cgtttgtgcc accaggctgt
      781 gtggtataat attggctggt tctagagcca gttgggcaga gtagaggcgg aagcctcagc
      841 gccattctcc ggcgctatcg cccggattga gcgcctgtag ctcagtggat agagcggcgg
      901 ccttctaagc cgcgggtcgg aggttcgaat cctctcaggc gtaccgatag atgcgggggc
      961 tgcagttccc gttcgtgtga tcccggtcgt tgcctctacc agattccccc tttctcgatg
     1021 acgttcaagc gctaactgtt tggcaggggt gaagtggcgc cgtttcttcg atgttgaaga
     1081 atcacgaact agccgcggca tgggagacaa ccggacgaac cggaggggag caagctgcgc
     1141 tccaatcgtg tctttgtgtg gaggttgatg gatcttgact gtctatagat cctctggccg
     1201 cacagagttg ttgggtttgg gcgattcggc gctggagagg gtggcaagga ttgcggggtc
     1261 caccctggat cgggaggaac tactccaagc cctgttcgtc gagttgaatc gaagcatccc
     1321 gtgtgactcg ctggggtttt attggcggga gccgggcgag agcttttgcc tggttatcgt
     1381 gcgcccaggc ttaaccgcaa tagaggttga gcagacccag gtagtcggag aagcgcccgg
     1441 agccgacgac aagcctcaac cttactgctg ccaggagaac tcctccagca cccatccggt
     1501 cttgcggtgg ttggccgccc acgacctctc ttcgagcctg aacgtcccag ttgtcgtggc
     1561 gcagcagttg ctggggtact ttgtcgccaa ccgaactggt agccacgctt tcgccgttgc
     1621 ggatcttcac ccgttggttc agatcgctgg gatggtagga ccctcactga actccatgca
     1681 gctgtatgag cggctcgagc tggcacacca gaacctgaag gccacgcgtg acgaactggc
     1741 aaggtccgag cggctgatgg tacagggtga gctggcttca ggcgttgctc acgacataaa
     1801 caacattctt gggttgatct ccgccaggac agacctgctt aagcttcagg gcctgccacc
     1861 tcaggcagcg gcttcggccg aggctatctc tcaggcagtg gacgacggca tcactgtggt
     1921 gcgccgcatg acccagttta cccgccgcca gcgcaagcaa gaggttgttc ccctcgatgt
     1981 gaacaagctg gtggatgacg tgatggagat gacccggcct cgatggcagt ccgcttcgag
     2041 cttgaataag gtttcggtgc gtttcacccc cgggccaatc tccacggtgc ttggtgttcc
     2101 ctccgaattg agggaggtca tggtcaatct ggtgatgaac gcggtcgatg cgatgccctc
     2161 gggtggttgg atacgcatcg atgtagagca ggccgatggt catgcctata tcagcgtcgc
     2221 cgataccggc tgcggcatgt cggaggaggt gcgggagcac atcttcgacc ccttcttcac
     2281 cacgaagggc gagtcgggtt ctggtttggg tctctgggta tcccacggga tagttgtgcg
     2341 ccacggtgga gacattgagg tgcacagcga ggtcggcaag gggagccgct tcagggtgag
     2401 gctgccgttg gccagggcgg ttcaggagca ggcaccgaag agggtggagt gtgtcgagta
     2461 ttccatcctc gtggtggacg acgaagccgg tctcggtgag gcgctgaggc tgtcactgga
     2521 gatggcaggg tatcatgtac tcttctcggc caatcctcgg caggcgttgg agatcttcaa
     2581 gcaggagcag ttcgaccttg tgatcaccga tctccggatg ccggggatgt ccggttggga
     2641 actggcttcg gagatcaaga ggttcaatcc cgctaccccc ataatcgcca tgaccgggtg
     2701 gcctgtggac ctgattcggg atgggcagcg atgctcggag atggaggcca tcatccagaa
     2761 gccctatcgg gtgaatgacc tgcgggctaa ggtagcccgt atcctagccg agcgtaagga
     2821 gaagccgtaa gagcctgagg gaggcggcgc tttccggtgt gctctcccct tctgtcgata
     2881 cagtaacagc ctcaggcagc cgtgttcatg aacgcgacga atgcctctac taccgagggg
     2941 tcgaattggc tgccggcgca cctcctcacc tcctctaccc cctgatctcg ggaccgtgcg
     3001 cgccgatagg gacggtccga tgtgattgcg tcgaaggcat ccgccactgc caggaccctg
     3061 gctagtagcg gaatttggtc gccggagagg gcgtagggat aaccggatcc gtcgaacctc
     3121 tcatggtggt atcggaccgc tgcaagccca cccccgagga accggagcgc ggacagcatc
     3181 ctgtaaccca tctccggatg ctgatggatt gccgctgcat cctcctgaga gagcggagtg
     3241 accttgcgaa ggacgttgtc ggggactccg atcttgccga tgtcgtgcag gagtgcggct
     3301 agctccagac tgcttctgtc catatccggg agccccatct ggagggccag cgaggacgca
     3361 atttctgcca cgcgtttgca gtgtccgtag gtgtagggat cacgggcgtc gacggcacct
     3421 gccagagcct cgagagttgc catgtaggtt tcctccagaa cggaccgtgt ttgagtgagc
     3481 tcctcgtggg ttcgcgcgag gtcatcacag tattcgttca actgtctctg ccagagagac
     3541 atctcgagta tcttggactc gacggcgctg gcatggctga gagttgcggt caccatcatt
     3601 acgttcttga ccgctgctgc aacctcagat gcgaagtagc tgagagactc ttcctgggcc
     3661 ggagcgaagg gcatatttcg ccaagcccac acctcaaggg ttcccaactc gtcgccgagg
     3721 cccgtcatcc tggctgtgat ggactctgat aggctcgcgg ccatagccgg gggaagttcg
     3781 gaaggagcat catcgtcggc ctctcgccag tagaccccgg cctgtcccac cacgagtgtt
     3841 gggccatcaa gcagggaaag cctgcaggcc cagccagacg tgatctccag ggcgcttctg
     3901 gctatcagtt cgaggatcgt tcgctggtcg aggcgatctt ccaaagcgat gttgaccctg
     3961 tgcagcgcgg ccttgaccag cgattggcta tcggtcacct cggcttggcc ggcctgccgt
     4021 aaggggcgtg tagaccgcaa caatatcccc agcgttccgg ccgtgatcgc tacggcgatc
     4081 aggcgtgccg tttccacttg gggttccacg tccaggacga gcagggcccc aaggctgatg
     4141 gcacagccaa tggctgggac atcgcgccgc agaagctttg ctgtccccgg ccagtgagac
     4201 gcccacgatc gccactggta tagtctggtg attgtcacgt tgatggtgcc gactccgttc
     4261 ccactattgg gtaaacgatt ccctagtagt ttagtcggta cccattgcca ctccgacatg
     4321 gtcatgggtc ccgaattgat cggggactgt ggtccctaat gggagggagt catgcactga
     4381 cgcagaggga tccggctttc gacgaccatc cccgtcaagt cagcggtggt tgagactgtg
     4441 ccgcctatgg gacgaacaag atctctgagc ttgtcgagaa gaggcggcgt tgcagcattc
     4501 tcgtcgaacg gcgggtgaag ggaggtgtgg ccatcgtccg cgactcgcat ggtgaggagg
     4561 tggcaggctg tttccacctc tacggctact ctagaggcgg aggcttgctt ggacaccgtg
     4621 gctagccttt cctgtatcac gccgaggatc gtcgatatcg cttgtggggg cagcgacacc
     4681 tccgagggta gtgcgagttc gacctccatg ccggtttgcc tggtgaagtc tgtgaccact
     4741 cgttgaatcg ctggcatgag gccgagccct gccgagtccg gtgagcgaag ggtattgatt
     4801 gaacgcctca actcctgaac gctcagttcg acgatggact ggagcttctc cagttccgcc
     4861 tttgcccgtt gtgggtccat gtcgatgaga cgggagataa tctccacttt gagcatgaga
     4921 agggctagat tctgggaaac gccgtcgtgg atctccgtgg ctatgcgact ccgttcttcc
     4981 tggatcacga gttcttgagc agccgcaacc agattggcgt tatgtacgac cagtgcagct
     5041 tgagtcgcaa gtgatgccag cagttgccgt tgatgaggcg taaaggacca ctgggtaggg
     5101 agcatcagcg tcacgatacc gacgcagcag ttgtgcgcgc tcagctttgc gatcacaact
     5161 gcgcctacat cttcgtccac gaagggcagt gcagtcggaa agtgctcgat gtcgatcacc
     5221 catagaccgg tccgcgcact gtgaatctca gtcaggcgct gcggggatag ggcggacaga
     5281 tccgcctcgc caatccagga gaagccgctg gtcgcgcgca gttgcggggc gccatctggc
     5341 gttcgagtgg tggccatcca gcttcccacg catgggaaga tccgtgcgaa cagggttaga
     5401 accgtctggc acgcctcgtc gatggataga atctccgagg ctagagagat catctcccgg
     5461 gagacggcag cttcttcctc gtccaaggac cgctgcgaac cgaccactgg ttactccctt
     5521 tacgcaggat ggatcgtgcc ggatggtaca ccgaacctat taggggcggc aagttttcga
     5581 gatggcttcg tccaaaccgg ccaagttatg gtattatgaa gaacacttcc cattgagatg
     5641 acaccgcaaa ctcccccact aatcctccac ggaggtcggc agtagtaata atgaagaaag
     5701 cacgcgttat ggttgccgat gaccataccc tgttcaggga agggcttcgg caactgctcg
     5761 aaatggagcg agatatcgaa gtggttggag aagcatctga tgggatggag gctgtggcaa
     5821 aggcgcggac tctcgtgccc gatgtcattc tgatggacat aaacatgccg gtagtggggg
     5881 gggtagctgc tatcggacag attctgtcgg agcgaccgga aactgggata atcgttctga
     5941 ccatgcaccg gcaggatcag tacgtcttcg atgccatgag ggcgggggcg aggggctatc
     6001 tcctcaagga cgccaaacct gccgacctga tcttcgccat tcgaatggtg gctcgcggcg
     6061 cgtcattggt ggatcccagg atgaccacca cggtactgaa ggagtttcgc cggcttgcca
     6121 gccaggtgga gccggaccag ggtgttgccg gactgacccc caaggaggtc gagatcctga
     6181 agttacttgc catgggtctc agtaacaagg agatcggccg tgagctttgc ctggccgaga
     6241 agacggtaaa gaactacctc tccacagtgt tccagaagct acagataaac gaccgagttc
     6301 aagctgcgat ctatgccctg aaacatgggc tgttgaccga gcaagagatc tcctgatcca
     6361 tggttcatgg cctcaccatt ggaacttcgg gccgtctcaa tacagtttgt cggagtctgt
     6421 aacaatatcc ccccgggccc gttactgtag atagatgggt gcctcgggca actcctgccg
     6481 tgggtcgcac gcatgagagt ggcctttcga cccgccctac atcaacctca ctccccgttt
     6541 gtcctccatt tcgggtgact ttctgcgcgt tttcgcgtgc cgcttggtcc gcaatacctg
     6601 ggactatggg ccctgatcga actgcctgtt ccacactatc gtatcggtgt tcgcgaaatc
     6661 ccccgacgaa tcctcattga atgtcgatgc gtcgcaagct gtggagcagg aggttggcct
     6721 tgagtagcgc agaaatggtt gccacgccag tggtgaccca gtcggtaatg cacacgttcc
     6781 aac
//
LOCUS       DATALS010000107         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_94766,
            whole genome shotgun sequence.
ACCESSION   DATALS010000107 DATALS010000000
VERSION     DATALS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94766"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VHS06_02270"
     CDS             <1..328
                     /locus_tag="VHS06_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986978.1"
                     /translation="PASSGRVAEQVDALGGEVSIWAHAIGALFVAEMMPTSGMIERVT
                     ALRGVAEAAGGYLVVENWVAQPGLKPWPGDVWGRPGGPLALMQAIKREYDPRRTINPG
                     RFVGGI"
     gene            336..1838
                     /locus_tag="VHS06_02275"
     CDS             336..1838
                     /locus_tag="VHS06_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX2986979.1"
                     /translation="MRKTRFAVGLAAGVALGVAAGRRADDMRRVLNGGNGKEVALRLG
                     RHGNGHETKAHHAVPKLEFPDRELLRQCVHCGLCLPFCPTYRALSLEADSPRGRIFQM
                     KLVADGEVSPNDPHFRKHIFQCLDCRACETACPSGVQYGRLIEAARSIVPAEGRAERG
                     ARSMVLGAALNSRAMLAVVGTGARAYQRSGLQAMVRGTGLLKPVAVLKRMEAMLPSLQ
                     GRLLAKQVPAYVPAQGEARHRVALLSGCIASQFFPETNLSTAAVLSANGCDVFALPEQ
                     GCCGALQNHSGDRKTALKMARHNIDVFESVHPDFIVVNSAGCGSMMKEYGDLLRDDSQ
                     YAERAKQFSAKVRDISELLVELPFRKPERELRMKVTYQDACHLAHGQKVRSAPQGDPE
                     VDTWAGDRGDEGLRPVLRERRHLQRDAAGVVGEDPGIEDEECPGDRRRDGGGWKSRLP
                     DTARAWAAGPGCDDGDSAPCGPIGPGVWPQGQSSCGVRSRVMGGATIGES"
     gene            complement(1990..2307)
                     /locus_tag="VHS06_02280"
     CDS             complement(1990..2307)
                     /locus_tag="VHS06_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX2986980.1"
                     /translation="MQLHAEDNTATALADLAAGSRCRIMLGTTRKEVVLVQDVKYGHK
                     FAVSAITPGEDVLKYGEVIGEASLAIAVGEHVHVHNVVSKRGRGDLSQGRSICSTGFV
                     SSR"
BASE COUNT          417 a          667 c          810 g          421 t
ORIGIN      
        1 acctgcgtcg tccggccgtg tggcggagca ggttgacgcg ttgggcgggg aagtgtccat
       61 ttgggcgcac gccatcggcg ccctcttcgt ggctgagatg atgcccacgt cgggaatgat
      121 cgagcgggtc actgcacttc ggggagttgc cgaggccgcc ggcgggtacc tggtcgtcga
      181 gaattgggtg gcacagccgg gtctcaaacc gtggcctgga gacgtgtggg gacgaccggg
      241 tgggccgctg gcgctcatgc aggcgatcaa gcgagagtac gatccgcgcc ggacgatcaa
      301 ccccggcagg tttgtggggg ggatctgaca cgaccatgag aaagacgcgt ttcgcggtgg
      361 ggctggcggc cggagtggct cttggagtcg cggccggccg gcgtgccgac gatatgaggc
      421 gtgtgctgaa tggtgggaac ggaaaggaag tggccctacg gttggggcgg cacgggaacg
      481 gccacgagac caaggcacac cacgcggtgc ccaagttgga gttccctgac agggagttgc
      541 tgcggcagtg cgtacactgc ggcctctgcc tgcccttctg ccccacctat cgggccctca
      601 gcctagaggc ggattcgccg aggggacgga tctttcaaat gaagctcgta gcggatggag
      661 aggtaagccc gaacgatccc cacttcagga agcacatctt ccagtgcctg gactgcaggg
      721 cgtgcgaaac tgcttgcccg tccggggtcc agtatggccg gctcatcgag gccgcacggt
      781 cgatagtccc cgcggagggc cgcgccgagc ggggtgcaag gagcatggtg ctgggtgccg
      841 cgctcaactc gcgggcaatg cttgccgtgg tcggaactgg ggccagagcc tatcagcgtt
      901 ccggattgca ggccatggtg aggggcaccg gtctgttgaa gccggtggca gtcctgaagc
      961 ggatggaggc catgttgccg tcgctgcagg gaaggctgct ggccaaacag gttcccgctt
     1021 acgtccctgc ccagggcgag gcgcgccatc gggtggcgtt gctttccggt tgcatagcca
     1081 gccagttctt ccccgagacg aacctctcca cggctgcggt gctttctgcc aacggctgcg
     1141 acgtctttgc cctgccggag cagggatgct gcggtgcgct gcagaaccat agcggcgacc
     1201 gaaagacggc gttgaagatg gctcggcaca atatcgacgt tttcgagagt gtgcaccccg
     1261 acttcatcgt cgtcaactct gccggctgcg gctccatgat gaaggagtat ggcgacctgc
     1321 tgcgggacga ttctcaatac gcggagcgag cgaagcagtt ctcggcgaag gttcgtgaca
     1381 tcagcgagtt gctggtggaa ctgccgttca ggaaaccgga gcgggaactg agaatgaagg
     1441 tcacctacca ggatgcctgc cacctggctc acgggcagaa ggtccgatcg gccccccagg
     1501 gagatcctga agtcgatacc tgggctggag atcgtggaga tgaaggactc cgaccagtgc
     1561 tgcgggagcg ccggcatcta caacgtgacg cagccggagt tgtcggagaa gatcctggca
     1621 tcgaagatga agaatgtcct ggcgaccggc gccgagatgg tggtggctgg aaatcccggt
     1681 tgcctgatac agctagagca tgggctgcgg gaccggggtg tgacgatggg gacagcgcac
     1741 cctgtggacc tattggccca ggcgtatggc ctcaaggcca gtcgagttgt ggggtgcgat
     1801 cacgagtgat gggtggagcc accattggtg agtcgtaggg cggggtctcg tgccccgcct
     1861 cctacgccat ggctgcgaca cacagttgct tttctgcgac gctttgggcg gggcacaaga
     1921 ccccgcccta cgagacgtca caggcgacgc acttcggtac agcgaccttc cctgctcgcc
     1981 cctggggccc tatcgggagg aaacgaagcc agtggaacag atggaccgtc cctgggatag
     2041 gtcgccgcgc ccgcgcttgc tgaccacgtt gtgaacgtgg acgtgctctc ccacggcgat
     2101 ggcaagtgag gcttcgccta tcacctcacc gtacttcaac acgtcttccc ccggcgttat
     2161 ggcagagacg gcgaacttat ggccgtactt cacgtcctgc accaggacga cttccttcct
     2221 cgtcgtgccg agcatgattc gacatcgact cccggcagcg agatcggcca gggcggtagc
     2281 agtattgtcc tccgcgtgaa gttggatcgc gtcga
//
LOCUS       DATALS010000108         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_10268,
            whole genome shotgun sequence.
ACCESSION   DATALS010000108 DATALS010000000
VERSION     DATALS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10268"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="VHS06_02285"
     CDS             complement(<1..1377)
                     /locus_tag="VHS06_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986981.1"
                     /translation="MGSKYHVKIAGQGFLVKPGSYRRTALSLLPSPSPQQSAPPVGWH
                     QWRQANWQGGDGRRNWDGETGRSENSGRWWAGYGVEVGEPGKVRLGPILSNSFTSTED
                     GFAAMLTFRERIYALPTSSGRIYSFDGTSWTVDWDSGKNAMASMARYRDQIYAGSGSD
                     GVLFARSGGGWANATQVNGAASVDCMASYEVWDPTARATVPRLFFGCSFTGGEAKVYQ
                     WDGTSLAELHSCMESKAEAMIVYGGRLYVATSDRGNGLQGRLLCFDGRSNSGEWSEAA
                     WLSDNFVAGWAIFDNLLFCGSGVGGKIWAFDGERMEEAYCLQTAGLEYREPLQALTVC
                     GGKLYTGYGHPTQGTALLCKLVTGAGGSRTGWYTPSAMGSGGLPEAMAVYGGSLYLGC
                     RATGAAPIYRKEADLFRSSGLLESSFFDAGQPEATKLLRTIALSHDKLLAGQGVEVKF
                     AIDGSDSFQ"
     gene            complement(1498..1875)
                     /locus_tag="VHS06_02290"
     CDS             complement(1498..1875)
                     /locus_tag="VHS06_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986982.1"
                     /translation="MAKVVTTGLTIANVPFGDTVKAGELVGWSNGKLVKACGVSSGPI
                     PAIGVAAASYQSDELGAVHLAGEVSGFTDLVAGDTQYLSLSAPGDIQSIPPSGAGSLM
                     QVVGYAVASDRLVVAIRDGGSYL"
     gene            complement(1973..2866)
                     /locus_tag="VHS06_02295"
     CDS             complement(1973..2866)
                     /locus_tag="VHS06_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX2986983.1"
                     /translation="MALLLTEAAKLSVDMLQRGVIETILKDSPILQLLPFMTVEGNAY
                     KYTQENTLGTADFYAVNATWSESTATFTQQSASLAILGGDADVDNFIQRTASNVQDQR
                     AVQVAKKAKAVAQKFEETFVSGDTLADPNGFNGIKKLISNSDQVVTAGANGASLTTAM
                     LDDLIDRVKGGKPDTLLMSKRSRRKLKGLLTASAHYVERGESSYGRQVMMYDGIPVEV
                     SDYISDGETQGSSNSCSSIYALSFGAEEALSGLQNGGIEVVDVGQLETKDANRVRIRW
                     YCGLALFNQLRCARLVGVLGN"
     gene            complement(2966..>3279)
                     /locus_tag="VHS06_02300"
     CDS             complement(2966..>3279)
                     /locus_tag="VHS06_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986984.1"
                     /translation="AEMERLKAELEETRTRWLEAHRRALLAENEGRVVPELVAGTSLE
                     ELERSVEVAKNAFDAARAAALAEIAATPVPAGNPIRREPDLESMSPMEKIAHGLKRSE
                     S"
BASE COUNT          524 a         1140 c          924 g          691 t
ORIGIN      
        1 ctggaaggag tcgctgccgt ctatggcgaa ctttacctcc actccctggc cggccagtaa
       61 cttgtcgtgg ctcagcgcaa tggtccgcag cagcttggtt gcctcgggct gtcccgcgtc
      121 gaagaaggag ctttccagca atccgctgct tctgaacaga tcagcttctt tccggtatat
      181 gggagccgct cccgtggctc tgcatcccag gtagaggctg ccgccgtaga ccgccatggc
      241 ctcgggcaat ccgccgctac ccatagcgga gggggtgtac catcctgtcc tggatccccc
      301 ggcacccgtc accagcttgc acagcagcgc cgtaccctgg gtcggatggc cgtatccggt
      361 gtacagtttg ccgccacata cggtcagggc ctgcaacggt tccctgtact ccagaccggc
      421 ggtttgcaga cagtaggctt cctccatcct ttccccgtcg aaggcccaga tctttccccc
      481 gactccagat ccacagaaca gcaggttgtc gaagatagcc cagcccgcga cgaagttgtc
      541 cgagagccag gctgcttcgg accactcccc cgagttgctc cggccgtcga agcagagcag
      601 tctgccctgc agcccgtttc ccctgtctga ggtagccacg tacagtcggc cgccatacac
      661 gatcatggct tctgccttag attccatgca gctgtgcaac tcggcgaggc tcgtcccgtc
      721 ccactggtaa acctttgcct ccccgcccgt gaagctgcaa ccgaagaata gcctgggcac
      781 cgtcgccctg gcggtgggat cccagacctc gtaggatgcc atgcaatcca cggaggccgc
      841 gccgttcacc tgagtagcgt tggcccagcc gcctccgctc ctggcgaaca gcacaccgtc
      901 cgacccgctg ccggcgtaga tctgatcgcg gtatcgggcc atggaggcca tagcattctt
      961 gccgctgtcc caatccaccg tccaactggt gccgtcgaag ctgtagatcc tgcccgagga
     1021 ggtgggtagg gcgtagatgc gctccctgaa ggtcagcatc gcggcgaagc cgtcctccgt
     1081 ggaggtgaag ctgttggaca ggatcggccc cagccgtacc tttccaggct cccccacctc
     1141 cacaccgtag cctgcccacc acctccccga gttctcggat cttcccgttt ccccatccca
     1201 gttccggcgg ccgtcgccgc cctgccagtt cgcctggcgc cactgatgcc agcccacggg
     1261 tggcgcggac tgctgaggac tgggtgatgg gaggagggac agggcagttc gccggtagct
     1321 gcccggcttc accaggaagc cctgcccggc tatcttcacg tgatacttgc tccccatgtc
     1381 cccctccgat tagctatcag ccatcagcga tcagatgccg actggcagca gttcgctgcc
     1441 atctgccagg tggttcagaa tagtgttttg cgacaactct ctacttgctg actgctctta
     1501 cagatagctt cccccgtccc gaatcgcgac taccagccgg tcggaggcta cggcgtagcc
     1561 caccacctgc atcaggctgc ccgcgccgct gggagggatc gattggatat cgccaggggc
     1621 ggatagactt agatactgtg tatcccctgc taccagatcg gtgaaaccgc taacctcgcc
     1681 cgccagatgc acggctccaa gctcgtcgct ctgataggat gcggctgcca ccccgatggc
     1741 ggggatgggg ccgcttgaaa ctccgcatgc cttaaccaat ttgccgttac tccagcccac
     1801 cagctccccc gccttcacgg tatctccaaa cgggacgttg gcgatggtga ggccggtcgt
     1861 cactactttg gccattcctt cccccttctg tgggggttgg gggttagggg ttggtagctg
     1921 tcacatccac cccccccccc atcccccgac ccccaacccc catctcccat cgttagttgc
     1981 ccaggacgcc gaccaggcgg gcgcagcgaa gctggttgaa cagggcaagg ccgcagtacc
     2041 agcggatccg taccctgttg gcgtccttcg tttccagttg ccccacgtcc acgacctcga
     2101 tcccgccgtt ctggagaccg ctcaacgcct cctcagctcc gaagctgagg gcgtagatgc
     2161 tgctgcaact gttggaactg ccctgggtct caccgtccga tatgtagtcg ctcacctcca
     2221 cggggatccc gtcgtacatc atcacctgcc ggccgtagct gctctccccc cgctccacgt
     2281 agtgggcgga ggcggtcagc aaccccttca acttccgccg gcttcgcttg ctcatcagca
     2341 gggtgtccgg cttgccgccc ttcaccctgt cgatcagatc gtccagcatc gccgttgtca
     2401 ggcttgcccc gttggccccc gccgtcacca cctggtcgct gttggagatc agcttcttga
     2461 tcccgttgaa gccgttggga tcggccaggg tatcgcccga gacgaaggtc tcctcgaact
     2521 tctgggctac cgccttcgcc ttcttggcca cctgcaccgc ccgctggtcc tggacgttgc
     2581 tggccgtgcg ctggatgaag ttgtccacgt ccgcgtcccc gcccaggatc gccaggctgg
     2641 ccgattgctg agtgaaggtg gcggtgctct cgctccaggt cgcgttgacg gcgtagaagt
     2701 ccgccgttcc cagggtgttc tcctgggtgt acttgtaggc gttgccctcc actgtcatga
     2761 agggcagaag ctgcaggatc ggcgagtcct tgaggatcgt ctctatcacc ccgcgctgca
     2821 gcatgtctac tgaaagcttg gccgcctcgg taagcagcag tgccatttgt ccctcctcgg
     2881 atgatgagtg acgagtgatg agtgatgggt gatgagtgat gggtggtgag tgctgagccc
     2941 ccactcatta ctcatgactc accactcatg actcgctgcg ctttagcccg tgggcgatct
     3001 tctccatcgg gctcatcgat tccaggtcgg gttcccttcg gatcggattg ccggctggga
     3061 caggcgtggc ggcgatctcc gctaaagccg cggccctggc cgcgtcgaag gcatttttcg
     3121 ccacttctac cgatcgctct aactcctcca ggctggttcc tgccaccagt tccggcacta
     3181 ccctgccctc gttctccgcc agcagcgctc gccggtgggc ctccaaccat cgggttctgg
     3241 tctcctccag ctcggccttc agcctctcca tctcggcga
//
LOCUS       DATALS010000109         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_144439, whole genome shotgun sequence.
ACCESSION   DATALS010000109 DATALS010000000
VERSION     DATALS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144439"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VHS06_02305"
     CDS             <1..138
                     /locus_tag="VHS06_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEX2986985.1"
                     /translation="GEQPRIIFLHYYGTGRADALARGFRAALDQLGKHGRTMPSRGMM
                     R"
     gene            166..1362
                     /gene="chrA"
                     /locus_tag="VHS06_02310"
     CDS             166..1362
                     /gene="chrA"
                     /locus_tag="VHS06_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX2986986.1"
                     /translation="MTSGSEGDTGGPSSARGRRPVGLRPFLLYFLRLGTLGFGGPIAL
                     AGYMQRDLVEERGWITSQDYLEGLALAQLAPGPLAAQLAIYLGWVRAGVMGATLVAAA
                     FITPSFLMVLAISGFYLRYGGLPWMQGVFYGIGAAVIAIIARSAVKLMRSTLRSNRLL
                     WALFITSAVTTAWTETEIVWIFVLCGVIALGAEALPHRTAAALGLAPWPLWLITGLHG
                     PAEPGTLWRIWMYFAQAGAFVFGSGLAIVPFLHGGVVGHFHWLSERQFLDAVAVAMIT
                     PGPVVITVAFIGYLVAGPVGAAAAALGVFLPCYLFVIIPAPYYHRVVGNRYVRAFVGG
                     VTAAAVGAIAGATLILGKRAVTDVPTAAIALLTLMVLSTTRKVPEPLLILVAGGIGIL
                     LSGRVR"
     gene            1405..1503
                     /locus_tag="VHS06_02315"
                     /pseudo
     CDS             1405..1503
                     /locus_tag="VHS06_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
     gene            complement(1709..2674)
                     /locus_tag="VHS06_02320"
     CDS             complement(1709..2674)
                     /locus_tag="VHS06_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986987.1"
                     /translation="MRTHTFEFRDHLDFADVLGGKCVVAVGIGPEREAILLTVDPESQ
                     TIPFERDKQSGTSFPRSVVEHPYPATVFRLKNSRVSRVDLPEVDAAFPHIQPLADDHT
                     LIVSARCHYRNGNPEQNAVIYDQHGQVIRCMVFGDGIQDVQVACDQRIWVSYFDEGVF
                     GNFGWPYPPMGADGLLCFDTDGQIVWQFHAPGGFDSMADCYALNVANDAVWICYYTNF
                     PVVQITSDGTVQGWENEFAGVAVLVIDRQRILLWGGYDKERNRCIVQAISGGKLVSPQ
                     RLKLCLPDGEPLDKARLIGRGSIIHAFAGTRWYQFDLRNLPTSFA"
     gene            complement(2792..>3118)
                     /locus_tag="VHS06_02325"
     CDS             complement(2792..>3118)
                     /locus_tag="VHS06_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX2986988.1"
                     /translation="PQDGETVIEKTLPCSFDRTTLHDFLQLRGISVLILAGLMTHMCV
                     ETTARGALPLGYAVIVAADACASRDLPSFDGQVIPHQEVHRNALAAMADRFADVLLTD
                     DVVKLA"
BASE COUNT          620 a          964 c          922 g          612 t
ORIGIN      
        1 ggggagcagc cgcggatcat cttcctgcac tactacggca ccggacgggc cgatgcacta
       61 gcccgtggct tccgcgccgc gcttgaccaa ctgggcaagc atggccgtac catgccgtct
      121 cgtgggatga tgcggtaagc atccttcacg atgggagcgg ctccgatgac cagcggctcc
      181 gaaggagata ccggcgggcc gtcgtccgcg agaggaagac ggcctgtcgg cctgcgaccg
      241 tttcttctct acttcttgcg cctgggcacc ctcggcttcg gggggccgat agccctggcg
      301 ggctacatgc agcgggatct ggtggaagag cgcgggtgga tcaccagcca ggactacctg
      361 gagggtttag cgttagccca actggcccct ggacctctgg ctgcgcagct tgccatctac
      421 ctggggtggg tacgcgcagg cgtgatgggc gcaaccctgg tcgccgcggc attcatcacc
      481 ccgtcgttcc tcatggtctt ggcgatctca gggttctacc tgcgctacgg cggcctcccc
      541 tggatgcagg gggtcttcta cggcatcggg gctgcggtga tcgccatcat cgcccgcagt
      601 gccgtgaagc tcatgcggag caccctacgc tccaaccggt tgctatgggc gcttttcatc
      661 accagcgccg tcaccacggc ctggacggag accgagatcg tctggatctt cgtgctgtgc
      721 ggggtgatcg ccctcggggc tgaggctttg ccacaccgca ccgctgcggc gttggggttg
      781 gccccctggc ctctctggct gatcaccggc ctgcatgggc cagcagaacc gggaacgctg
      841 tggcggatct ggatgtactt cgcccaggcg ggagcattcg tgtttgggag cgggctggca
      901 atcgtgccgt tcctgcacgg cggggtggtc gggcatttcc actggctgag cgaacggcag
      961 ttcctggacg ctgttgcggt cgcgatgatc actcccggcc cggtagtgat caccgtcgcc
     1021 ttcatcggct acctggttgc ggggccggtg ggagccgctg ccgcggctct gggggtcttc
     1081 ctgccctgct acctctttgt catcatcccg gccccttact accatcgggt cgtcgggaac
     1141 cgctacgtcc gagccttcgt cggcggcgtt accgccgccg cggtaggagc tatcgcaggg
     1201 gctaccctga tcttggggaa gcgggccgtc acggacgtgc caacggcggc gattgccctg
     1261 ctgaccctga tggtgctctc tacgacgagg aaggtgccgg agccactcct catcctcgtc
     1321 gccgggggca tcggtatcct gttgtcgggc cgggtgcggt aagtcgaggc acggccctac
     1381 accagatcga agactgcctt caccgtcagc cccagcccca ccagcgtgat cacccacctg
     1441 gccttctatg ccgggtggcc gaaggcgatg tccaccatca cggttgcgaa ggaagtcttc
     1501 aagaagtgac gcctcgtagc catcgacacg tttaaaagcg aaaggagaag agatccatgt
     1561 tgacgcgcaa tcgttcaccc tctcgccacc tatctgctcc aagcggttag tttgggaggg
     1621 aggagtaggc acgtcgtatg gcaccccggc agccaacgcg tcggcaccga gcgtgacgat
     1681 cctcagcgta gatgcctctg tcggcgatct aagcgaagga agtcggcaga ttccgtaaat
     1741 caaattggta ccagcgggtc ccggcaaatg catggatgat cgagccacgc ccaatgagcc
     1801 gtgctttatc gagaggttca ccgtcgggaa ggcacagttt caaccgttgc ggtgacacta
     1861 acttcccgcc ggatatggcc tgaacaatac atcggttgcg ttccttgtca tacccacccc
     1921 acagcagaat acgctggcgg tcaatcacca agacggctac accagcgaac tcattctccc
     1981 acccctgcac agtgccgtct gatgtgattt gcacgaccgg aaaattggtg tagtagcaga
     2041 tccatacggc gtcgtttgcg acattgaggg cataacaatc tgccatcgaa tcaaagccgc
     2101 caggagcatg aaactgccag acgatttgcc catccgtatc aaaacagagc aatccatctg
     2161 cacccatcgg tggatacggc caaccaaaat tgccaaagac cccttcgtca aagtaagaaa
     2221 cccaaatccg ctggtcgcac gcaacttgaa catcctggat gccatcgcca aacaccatac
     2281 agcggatcac ctgcccatgc tggtcataga tgacagcatt ttgctcgggg ttgccgtttc
     2341 tgtagtggca gcgcgcggaa acgatgagcg tgtggtcatc agctaatggc tggatgtgtg
     2401 gaaaagctgc atctacctcc gggagatcta cacggctcac acgcgaattc ttgaggcgaa
     2461 agacggttgc tggatatgga tgctcaacca ccgaacgagg aaatgatgtc cctgattgct
     2521 tatcgcgctc aaacggaatc gtctggctct ccggatccac tgtaagtaaa atcgcctcac
     2581 gctccggtcc gattccgacc gcaactacgc acttcccgcc cagtacatca gcaaaatcca
     2641 gatggtcacg gaattcgaag gtgtgggtcc tcaaggcttc tgctctcccg ccggttgaac
     2701 cgatagttca tggaagatca ataccaagaa atagagagtg aaagggggca ctgcgcgagc
     2761 acccccggaa gtcatcctcg ccgccattcc tctacgccaa cttcaccaca tcatccgtca
     2821 acagcacgtc ggcaaaacga tcggccatgg cagccagggc gttccgatgc acctcctggt
     2881 gggggatcac ctgaccatcg aaggaaggca aatcgcggct ggcgcaggcg tcagcggcta
     2941 cgatcaccgc gtagcccagt ggcagtgcgc cgcgcgcggt agtttccacg cacatgtgcg
     3001 tcatgagccc ggctaaaatc agcacggaaa taccgcgcaa ttgcagaaaa tcatgcagcg
     3061 tcgtgcggtc aaagctgcag ggcagcgtct tttcgatcac cgtctcccca tcctgagg
//
LOCUS       DATALS010000110         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_410860, whole genome shotgun sequence.
ACCESSION   DATALS010000110 DATALS010000000
VERSION     DATALS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410860"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1884
                     /locus_tag="VHS06_02330"
     CDS             <1..1884
                     /locus_tag="VHS06_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX2986989.1"
                     /translation="VLGGATDYQVVSEKLNQVKVGNTGYSYMVDRTGLVVAHKDAKNI
                     LATNVTTNSSPELAAIGNRRIKGEAGYGSYPFEDSNRIIGFAPVKGSGDYKAQGLAVA
                     AACQESEYLQPLGNLLRSSLAVASVLLLLALGLAYLLAKTVSEPLEALTDVARHTAVG
                     DVKYHVDAKKWQKTVRFDEIGELGLAFRAIRDYQKVMAKQTQELADGDLTVQVQSHGE
                     KDLLGNAFAEMVGKLRHSILKVTEVTDNLAEAGRQLSVASEGAGSVTQQIATSIQQVA
                     AGNQEQSSAVQETTQSVNDLSAAIDQIARGSQEQSDSIQQASISVTQLNESVAQASAA
                     SQQVSAATSEAQRVAAEGAEVVRKAIRGMDLIKDQTESTASEVQDLTKYSAQIGSIVE
                     TIDDIAEQTNLLALNAAIEAARAGEHGKGFAVVADEVRTLAERSSKSTKEIAYLIMEL
                     RREIEEAVAAARQGSAKVETESKLAQEAGEALKSILISVDGAREQVERIATAVNQMES
                     ASRQVVQMMDAVSAVAEESSSASQEMARSSREVENAMGKIASVSEESSAAAEEVSAAT
                     EEMASQVEEMVGNAHSLAQMAEQLQATVAQFELGGDAGVATQRLSKVWTTPSEAKPVA
                     KAG"
     gene            complement(2093..2653)
                     /locus_tag="VHS06_02335"
     CDS             complement(2093..2653)
                     /locus_tag="VHS06_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX2986990.1"
                     /translation="MEQDHEIIIAGFGGQGVLFAGMVLAHAAMRCGLNVAWIPSYGPE
                     MRGGTARCTVIISKEEIGSPIVNHPSAAVVMNEPSLAKYGPRVLPGGTLVINRSLARA
                     PFTRPDIRIVEVGANEVAAELGSERAANMVALGALLGSVGCLAVEDVEAAMPEALGPV
                     KERFVEVNCGAIRRGIALVREQVAVP"
BASE COUNT          633 a          855 c          879 g          463 t
ORIGIN      
        1 gttctgggcg gggccacgga ttaccaggtg gtgagcgaga aactcaatca ggtcaaggtc
       61 gggaacaccg ggtactccta catggtggac cgcacagggc tcgtggtagc ccacaaggac
      121 gcgaagaaca tactggccac caacgtcact accaactcct cgccggaact tgcggccatc
      181 ggcaacagga ggatcaaggg tgaggctgga tacggaagct atccgttcga ggattccaac
      241 aggataatcg gctttgcacc tgtgaaaggt agcggcgact acaaggcaca gggattggcg
      301 gttgccgcag cctgccagga gtcggagtac ctgcaaccgc tggggaacct gcttcgctcc
      361 agcctggcag tagcgtcggt gctgttgctg ctggcacttg gcctggccta cctactggcc
      421 aagactgtat ccgaaccact ggaagcactc accgatgtgg cgcggcacac ggcagtgggc
      481 gacgtcaagt atcacgtcga tgcgaagaag tggcagaaga ccgtcaggtt cgacgaaatc
      541 ggggaactgg ggctcgcctt ccgagctatc cgagattacc agaaggtgat ggcaaagcaa
      601 acgcaggaac tcgccgatgg cgatctgacc gtgcaggtgc agtcacatgg ggagaaggac
      661 ctgctcggca acgccttcgc cgagatggtg ggcaagctac ggcactctat actcaaggtg
      721 acggaggtga ccgataacct ggcagaagcc ggacgccagt tgtcggtcgc gtccgaggga
      781 gccggttcgg tcacacagca gatcgccacc agcattcagc aggtggcagc cggcaaccag
      841 gagcaatcga gtgcagttca ggagaccacc caatccgtca acgacctctc agcggccatc
      901 gatcagatcg cccgcggatc ccaggagcag tccgactcca ttcagcaagc ttcgatctcc
      961 gtcactcagt tgaacgaatc tgtagcacag gcatctgctg cctcccagca agtttcggcg
     1021 gcaacttccg aggcacaacg agtggccgcc gagggagccg aggtggtgcg caaggcaatc
     1081 cggggaatgg acttgatcaa ggaccaaacc gaatccactg catccgaagt ccaggacctg
     1141 accaagtact ccgcccaaat cggttcgatc gtcgagacca tcgacgacat cgcggagcag
     1201 accaacctgc tggcgctcaa cgcggcgatc gaggcggcca gggcgggaga acacggcaag
     1261 ggcttcgccg tggtcgcgga cgaggtgcga acgcttgccg agaggtccag caagtccacc
     1321 aaggagatcg cctacctcat catggagttg cggcgcgaga tcgaggaggc cgtcgcagcc
     1381 gccaggcagg ggtccgcgaa ggtggagact gagtccaagt tggcccaaga agcgggtgag
     1441 gcactcaaga gcatactcat ctcggtggac ggtgccagag aacaggtgga acgcatcgca
     1501 acggccgtaa atcagatgga atcggcctca cggcaagtcg tgcagatgat ggatgccgtg
     1561 tcggccgtgg cggaggagtc atcgtcggca tcacaagaga tggcccgctc cagtcgtgag
     1621 gtcgagaacg cgatggggaa gatcgcctcg gtgagtgagg agtcgagtgc cgcggccgag
     1681 gaggtgagcg ccgctaccga ggagatggcc tctcaagttg aagagatggt gggcaacgcc
     1741 cacagcctgg cgcagatggc cgagcaactg caggcaacgg ttgcgcaatt cgagttgggt
     1801 ggagacgccg gcgtcgcaac gcagcggctc agcaaggtct ggacaactcc ctccgaggca
     1861 aagcctgtag ccaaggctgg gtgaaatcgg cgcatgcccg ccaaatgtga tgcccccacc
     1921 agggcatgtg taactcccag tgtcaagatc agcgtgtcag aggactaata ctcgaccaca
     1981 gtgattctgg aaggtgtcat cctgagcgac agcgaaggat ctctccgttc acgaggagat
     2041 gcttcgcttc gctcagcatg acagtacgtg tcacttctac ggtggcggtg cactaaggga
     2101 cggcaacctg ctcccgcacc agggcaatgc cgcgccgaat cgcgccgcag ttcacctcta
     2161 caaagcgctc cttcaccggc cccaacgcct cgggcatcgc agcctccaca tcctcgacgg
     2221 caagacagcc aaccgacccc aacagcgcac ccaaggccac catgttggcg gctcgttcgc
     2281 tgcccaactc ggcggccacc tcgttcgccc caacctccac gatgcggatg tcaggtcggg
     2341 tgaagggtgc gcgcgccaga gaccggttga tcaccagcgt gcccccggga aggactcggg
     2401 gaccgtactt cgccagggag ggctcgttca tcaccaccgc agccgaggga tggttcacga
     2461 taggggagcc aatctcctct ttggagatga tcacggtgca tctggcggta ccgcccctca
     2521 tctccggtcc ataggaaggt atccaggcaa cgttgagccc acaccgcatg gcagcatgcg
     2581 ccagcaccat gccggcgaag agcacaccct ggcctccaaa ccctgcgatt attatctcgt
     2641 ggtcttgctc cacgtccctc acctccccta gataggtagt agaaagaaac agtaaatttg
     2701 gggacacccg cgaaggctgc gctcgctcgc cttcgccccc gccaggcgct acgccctgga
     2761 cccgtttctt tctacgacgt acttagatgt cccgtgcggc aggggtcacc ttgatgtcac
     2821 ccagcggata
//
LOCUS       DATALS010000111         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_95163,
            whole genome shotgun sequence.
ACCESSION   DATALS010000111 DATALS010000000
VERSION     DATALS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95163"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            503..1213
                     /locus_tag="VHS06_02340"
     CDS             503..1213
                     /locus_tag="VHS06_02340"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX2986991.1"
                     /translation="MEQGTWEKVERTNSYEAIVEQVRHMIDSGVVRPGDRLPAERELA
                     GKLAVSRATLREAFRVLEHTGLVESKIGQGRFVAQIKPYGAVTSGTARSLEGVAILDF
                     LEVRRSLEVGMAGLAAERASAEEIANMEASLYKVPENEEVDLKADTGFHMTIAEATHN
                     GVYKRFFSSELFLLYRLATTIRLGWEWRNYNNPEHRDVLEAIRCHDRPAAEAAMLHHI
                     SSMENRLRFVLSHPAAQR"
     gene            complement(1489..>1955)
                     /locus_tag="VHS06_02345"
     CDS             complement(1489..>1955)
                     /locus_tag="VHS06_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986992.1"
                     /translation="GDRSVQDLKLVFASSSSDEKIEFDLGTLSQSTSITAGAGAAGLK
                     TVDASVLPAELKSIPMPPGFGVVDGSPSRSASGGAFQRAIAKLFGKMSIKDLAQFYGT
                     ALAKEWEANNVDSDTGHLQGEFVNKKDGNLILYINAEQSDKGTTVDIMVEKN"
BASE COUNT          411 a          584 c          592 g          368 t
ORIGIN      
        1 atcgagggat cgccagcttc gtccagtgat gaccagtccg gcaaccgacc agtgaaaacg
       61 tgttgacctc gccatcgagg aacaccaccg gaggggttgg aggccgccgc gccggtggac
      121 cagtgggcga gtggcctagc cactgaccca gatcgtagta caacaaagtg ggggtgtcaa
      181 gcgtttttgg gcagtctgta gggccttgca gacaaaaacc atccgtgcta ggatgggcca
      241 gtggctagtc cactggccca cagcatctcg ttgctgagcc cggtcggttc tgcaagaacc
      301 gctcagcaag actcgacgac ccgtatctct cgatcctgcc ccaagagtgg gatccattga
      361 gcgtacgatc caccttgcga agccggcacg ggccgcctgc ttctcgcctc agactgatcg
      421 tccttcgagc gccacgcatc gatggaggtc aacggtggag acctgacgat ttgacgtttc
      481 agattggagg ctgacgatag gcatggagca gggcacgtgg gaaaaggttg aaaggacgaa
      541 ctcatacgag gccatcgtcg agcaggtgag acacatgatc gacagtggag tagtgcgccc
      601 cggcgatcgt ctgcccgcgg agagggagct tgcaggcaag ctagcggtga gcagagccac
      661 cctgagagag gcttttcggg tgctggagca cactgggctg gtcgaaagca agatcgggca
      721 agggcgattc gtggcgcaga taaagcctta cggtgccgtg accagcggca cggccagatc
      781 gctggagggg gtagccatcc tggacttcct tgaggtgcgc cgttccctgg aggtcggtat
      841 ggcagggctc gccgcggaac gagccagcgc agaggagatt gcgaacatgg aggcatctct
      901 gtacaaggtc ccggagaacg aggaggtcga tctcaaggcc gacaccgggt ttcacatgac
      961 catcgcagag gctacccaca acggggtgta caagcgcttc ttctcctccg agctgttcct
     1021 cctctaccgc ctggccacca ccatcagact ggggtgggag tggcgcaact acaacaatcc
     1081 agagcaccgc gacgtcctgg aagcgatccg ctgccacgac cgacccgccg ctgaggcagc
     1141 gatgctccac catatcagca gcatggagaa tcggctacgc ttcgtcctga gccatccggc
     1201 cgcacagcgt taagcgcagc ctctgcaaga ggcgggcgcc aacagatacc cggccggaca
     1261 agaagaaggg ctactggaac ctccgatcgg aaattccagt agccctttct tcgacagaga
     1321 cacgtgggag tccaacccac ccgggatggc gactattgtc cggcaaacgg tattggagcc
     1381 ttcgatctcc gctagggtcg gtccaccatg accccggacg gcctgccgtg cccggccgcc
     1441 acagaccctt tgcggagggt cagatcaagc gatccatctt gtggcttctc agttcttctc
     1501 caccataata tcgaccgtgg tgcccttgtc tgactgttcg gcattgatgt acaggatcag
     1561 gttgccgtct ttcttgttga cgaattcacc ctgaagatgc cccgtgtcac tatccacgtt
     1621 gttagcttcc cattccttcg ccagggcagt accgtagaac tgcgccaagt ccttgatgct
     1681 catcttgccg aacagcttag caattgcacg ctggaaagct cctccactgg cgctacgact
     1741 ggggcttccg tccaccacgc caaagcccgg cggcattggg atggacttca gctcagcagg
     1801 gagtaccgag gcatccactg tcttgagtcc ggcagcgcct gccccggcag tgattgacgt
     1861 gctctgcgac agggttccga ggtcgaattc gatcttctcg tccgaagaac tggaggcgaa
     1921 gaccagcttg aggtcctgaa cgctgcgatc ccccg
//
LOCUS       DATALS010000112         8572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_95166,
            whole genome shotgun sequence.
ACCESSION   DATALS010000112 DATALS010000000
VERSION     DATALS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95166"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..394
                     /locus_tag="VHS06_02350"
     CDS             272..394
                     /locus_tag="VHS06_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2986993.1"
                     /translation="MPENGRKDGALNFGIIGCGRIASRHADALWARGDTRLVAV"
     gene            407..640
                     /locus_tag="VHS06_02355"
                     /pseudo
     CDS             407..640
                     /locus_tag="VHS06_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            680..1330
                     /locus_tag="VHS06_02360"
     CDS             680..1330
                     /locus_tag="VHS06_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2986994.1"
                     /translation="MRRLREAADAGKLGKLLLSTVTVRWYRPQEYYSGDNWHGTWAMD
                     GGAMMNQAIHHIDALQWMMGDVDSVFAYTATLAHTMEAEDTGVGVVRFKSGSLGSIES
                     STITYPANIEGSLAVFGERGSIKIGGNSLDRIAFWKVEGEVEHEQEILRRQELDPPPS
                     RYLSHSLVLDDMVKAIREDRQPATNGVEGRKSLALVLALYQSARECREVKMEEVEE"
     gene            1327..1974
                     /locus_tag="VHS06_02365"
     CDS             1327..1974
                     /locus_tag="VHS06_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX2986995.1"
                     /translation="MISPTARIYPNVLLGENVEIGDFVIVGVPPKGKAEGELPTIIGD
                     GAVIRSHTVIYAGNVIGNGFQSGHHTLIREENRIGNNVSVGSSSVVEHHVEIADRVRI
                     HSQAFVPEFSVLEEGAWIGPNVVLTNAPHPLCPRAKDCLKGPTIKRGAKIGANSTIIP
                     FIVVGEDALVGAGSVVVKDVPDRAVVAGNPARVLKSIDDLTCPVDLIDSPYGGKR"
     gene            1971..3071
                     /locus_tag="VHS06_02370"
     CDS             1971..3071
                     /locus_tag="VHS06_02370"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX2986996.1"
                     /translation="MKIPLCDLKAQYATIQKEIDDAIQSVISNTSFIQGKECRLLEQQ
                     FAAFSGAQYGIATSSGTDSLHLALLALGVGPDHEVITTPLTFTATAEAISHCGGTPVF
                     VDIDPKTYNMDPARLESKITKRTKVILPVHLYGQPVDMDAVLAVADRHGIAVLEDAAQ
                     AHGAEYKGKRIGSIGSAACFSFYPGKNLGAYGDAGMITTNDKKLADWCSMMGDHGRTE
                     KYSHDYIGYGKRMDTIQAAVLLAKLRHLDEWTAARRERAAIYDRMLAGVVETPYQPEW
                     AKSVYHQYVIRVPNRDDVFNRMKEAAVGVGIHYPIPLHLQPAFRSLGHRKGDFPNAEK
                     AADSVLSLPIFPEMTLEQQSYVVDQLKAALKR"
     gene            3086..4189
                     /locus_tag="VHS06_02375"
     CDS             3086..4189
                     /locus_tag="VHS06_02375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2986997.1"
                     /translation="MRILFVTQIVDRSHDNLGAVHGWIDALARQVETVHVLALNTGVV
                     DLPPNVFVHSLGKDLGNGKLRQLQIFNSVAYSLALRRQVDVFCPHMVPRYALLVAPYR
                     LLFRIPTVMWYAHNSADLKLKSANLCVDRFVTVTRDSFPLGSKKCSVVGHGVDTSLFR
                     PAPESTATIDGKVRFLSLCRISPRKELDTFVSAADLVVNRWGQTKVQFIIAGGPLAPE
                     DHTYLQHLKDRVKELKLEEYFLFTGGIPHSETVHRLQSCDYFVNMHVEGGLGKAVLEA
                     MSCGLPAFVSTPTYYEHFPDYGKQFLFAPRDPVSLAERMKATLEMSAADRIRIGADLR
                     DWVCREHDLKLQMSRIARVMEEVKAGRRPSTFE"
     gene            4234..5868
                     /locus_tag="VHS06_02380"
     CDS             4234..5868
                     /locus_tag="VHS06_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX2986998.1"
                     /translation="MSTLLSTLGISHEFVREGEIDGSERLAGRIHLVIGEADGNLMNY
                     LCREAAVVVLPEPDFVPSAVTQNPAPSTQHPALEYDPDTFRLISRPTRNGYVVGIAAD
                     LVGLAFRLPARGEEYAVPTRDTHDRFQWDYSSLSKSYRGPRPLVSEYSHLLLDCFLEC
                     SKHTKQPMIRKEFWPNGKPMAGCLTHDVDVVKRGKLGHGVAVRDVKGALSSLMTGRLG
                     RAAGQISTIATTATSSKDPYWTFDRIAAMEEAHDFRSTYYFMADNRHPEDAVYTLADP
                     SISDLMAKLEGNGCEIGLHGSYASYTDAEMLSSLKTELEQKLGHAVIGHRNHFLRFRV
                     PDSWKAQEAAGFTYDATLGFADHEGFRGGHAFPFVPYDLATDRPIGLVEVPLAVMEVS
                     LSKYRKLRGEDAAGAIQTVLRQATAVGGLATLLWHNDSFFDPESPGVGALYERSLDWL
                     AEENAHVAPVQEIVAWWKSREAVRLIPLEDGRNGWRMEAPEEIDGLVLRIGLPDAGSS
                     LRVHGQVPLALRRDGPDHLLEYGPLPAGFSMDIEYR"
     gene            5865..7040
                     /locus_tag="VHS06_02385"
     CDS             5865..7040
                     /locus_tag="VHS06_02385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2986999.1"
                     /translation="MRLIYLANNRIPSEKANSLQIMQMCRAFQQEGAEVRLVVPHRMQ
                     PKVMRGVTDPFAYYGLPDRFPIVHLPCADTLEIAPGRLQPAAFALQSSTFCVSVAGYL
                     VGHKADLYYSRDPLSTLLLGRAPESVRSRSVYEAHTFPKPGPRQRAHVDSIARMRGLV
                     CITRGLANEYEALGIPRARILVAPDAVELERFASMPDRDEARRELGIPTEARVVCYTG
                     HLYAWKGAHTLALASRHLPMDHLTYIVGGTAEDLSRFVQFLAEEALDRVKATGHVPPD
                     MVPTYLAAADVVVLPNSGQSAASSRYTSPMKLFEYMAARRPIVASRLPAIEEVLRHGD
                     TGWLVDADDPEALAAGIKVMTSSSELMRGLASRAYAEVQQYSWKSRAKSIMDFFSED"
     gene            7041..>8572
                     /locus_tag="VHS06_02390"
     CDS             7041..>8572
                     /locus_tag="VHS06_02390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2987000.1"
                     /translation="MVSGSPTAQSRAGRTDSEASGAKAPAMVAWNVLVAASTALAGCL
                     VLLGRALPPQATLWWLSLYALIFALSMVWLSFYANRVPNRYGATRHLLLLETPILLLS
                     LGGSLALYLSDDLNHQLNYFLQPTPAVVLLLAVLSTAIAQEALLDTSSVGLRPRLLAS
                     LARARRWLLPTSLVLIGLLQGSSYLWIVGNDFIRYWAVADATRSWMGYPATAHLPSYV
                     EGGMYPYSIELPAFPMLLMISFMLAGHDTLAAHLPALAANCVLPPIVYAFFRRAGFGR
                     TLSYAAACCMVLFPFLRLYTLNAPVPDAVFVALLTFTGYLFLGLIGTNRRGSGTLADW
                     VGRLNDSSPGSAPNPQPPTLNPQRWAAFGLAAGLTTLTRPEGLLFVGCMFLALIANLL
                     KPRLYLAGAIFLAGVVPFSLLMLSTFGIPWARNADSSFGLENIVGNLNWINGMTLPFF
                     ADPFRLPYPVFIALVALMVLAMGLGTLWIATKSWQMAALPAAAAFHIFGVFTVDPRVS
                     GV"
BASE COUNT         1643 a         2711 c         2673 g         1545 t
ORIGIN      
        1 gggaacgata cgatcgccag cacaacctgg cggagatggt gcggatgtgg cgggccaccg
       61 cagcggcaca caagcggtag agccgttgcc cctgtaacgg gtggaggcgc acctgcgaat
      121 ccatgacggt aggggcgcac ctgcgtgtgc gctcgaggga ttccactacg cccactgatc
      181 cgcaaaccta gcacggcgca tgtgcatcct ctgcacaccc tggcggacac ataggtccgc
      241 ccctaccgga acgctgttga ggtgaattgt gatgccagag aacggccgca aagatggggc
      301 attgaacttc gggataatcg gctgcggtcg aattgcctcc cgccacgccg acgccttatg
      361 ggcgcgaggc gacaccagac tggtggcagt ctgagacgag agcgagcagc gagcagccaa
      421 gatggcctcg cagttcgggt gtgacgcata ctccgacctg gaccagatgc tggccagacc
      481 cgacttggac gtggtgtgca tctgtacacc cagtggcctg cacgcccctc aggggatccg
      541 ggcggccagg gcaggaaagc acgtcatcgt cgagaagccg atggccctct cgctcaagga
      601 cgccgatgcc ttggaggccg cctgccggga gtcgggcgtc aagctgacgg tggtgctgca
      661 gaaccgctac aataccgcga tgaggcggct gagagaggca gccgatgcgg gcaagctggg
      721 caagctgctg ctgtccacgg tgaccgtgcg ctggtatcga cctcaggagt actactccgg
      781 ggacaactgg catggcacct gggcgatgga cggcggcgcc atgatgaacc aggctatcca
      841 ccacatcgac gccctgcagt ggatgatggg cgacgtggat agcgtgttcg cctacaccgc
      901 aacgctcgcc cacaccatgg aagccgagga caccggcgtg ggcgtggtgc ggttcaagag
      961 cggcagccta ggatccatag agagctccac catcacctat ccggccaaca tagagggctc
     1021 cctcgcggtc ttcggcgaga ggggctcgat aaagatcggc ggcaactccc tggaccggat
     1081 cgccttctgg aaagtggagg gcgaggtgga gcacgagcag gagatcctgc gccgccagga
     1141 gctagacccg ccgcccagcc gctacctctc acacagtctg gtattggacg acatggtaaa
     1201 ggccatccgt gaggatcgcc agccggcgac caacggcgtc gaagggcgta agtcgctggc
     1261 gctggtgctg gcgctgtacc aatcggcccg cgagtgtcgc gaggtgaaga tggaagaggt
     1321 cgaggaatga tcagtcccac ggcaaggatc tacccaaacg tgctgttggg cgaaaacgtg
     1381 gagataggcg acttcgtcat cgtgggtgtg cccccaaagg gaaaggccga gggcgagttg
     1441 ccaaccatca tcggcgacgg tgccgtgatc cgctcccaca cagtgatcta tgctgggaac
     1501 gtcataggca acggcttcca gagcggacac cacaccttga ttcgagagga aaaccgaatc
     1561 gggaacaacg tcagtgtggg cagcagctca gtagtggagc accacgtgga gatcgccgat
     1621 cgggtgcgta tccactccca ggccttcgtg ccggagttct ctgttctgga agaaggggcc
     1681 tggataggtc ccaacgtcgt cttgaccaac gcgcctcacc ccctttgccc tagagcgaaa
     1741 gactgcctca aggggcccac gatcaagcgt ggcgccaaga tcggagccaa ctccacgatc
     1801 atccccttca tcgtcgtcgg ggaggatgca ctggtggggg ccggatcggt cgtggtcaaa
     1861 gacgtcccgg acagggcagt ggtggcaggc aatcccgcca gggtgctaaa gagcatcgac
     1921 gacctcacct gcccggtgga tctaattgac agtccctatg gaggtaagcg atgaagatcc
     1981 cgctgtgcga cctcaaggcg cagtacgcca ctatccagaa ggaaatcgac gacgccatac
     2041 agagcgtcat ctccaacact agtttcatcc agggcaagga atgccggttg ctcgagcagc
     2101 agttcgcggc cttctccggt gcccagtacg gcatcgccac cagttccggc accgactcgc
     2161 tgcatctggc actcctggcg ctgggcgttg gcccggatca cgaagtgatt accacgcccc
     2221 tcaccttcac tgccacggca gaggcgatct ctcactgcgg cgggaccccg gtgtttgtgg
     2281 acatcgatcc caagacctac aacatggacc ctgccaggct ggagtccaag atcaccaaac
     2341 ggaccaaggt aatcttgccg gtgcacctct acggacagcc agtggacatg gacgcggtcc
     2401 tggcggtcgc cgatcgtcat gggatcgcag tgctggagga tgcggcccag gctcacggcg
     2461 ccgagtacaa gggcaaacgg atcggcagca ttgggtcggc ggcctgcttc agcttctacc
     2521 ccggtaagaa cctgggggca tacggcgacg cggggatgat caccaccaac gacaagaagt
     2581 tggccgattg gtgctccatg atgggcgacc acggccggac cgagaagtac tcccacgact
     2641 acatcggcta cgggaagaga atggacacca tccaggcggc cgttctcctg gctaagttgc
     2701 gccacctgga cgagtggacc gccgcccgca gggagcgcgc agcaatctac gacaggatgc
     2761 tggccggagt ggtggagact ccctaccagc ccgagtgggc caagtcggtc tatcaccagt
     2821 acgtgatccg cgtccccaat cgcgacgacg tgttcaaccg aatgaaagag gccgcggtgg
     2881 gagtcggcat ccactatccc atacctctcc acctgcagcc ggccttccgt tctctggggc
     2941 accgcaaggg agatttcccc aacgccgaga aggctgcgga ttcagtgctc tcgttgccca
     3001 tcttccccga gatgacgctg gagcagcaga gctacgttgt ggatcaactc aaggcagccc
     3061 tcaaacggta gcaatcggat agtagatgag gatactgttc gtcactcaaa tcgtggaccg
     3121 ctctcacgac aacctgggag cggtccacgg atggattgac gcccttgcca gacaggtcga
     3181 gacagttcac gtcctggcac tgaataccgg cgtcgtagat ctgccaccca acgtcttcgt
     3241 ccactctctg ggcaaagacc tgggaaacgg caagttacgc caactgcaga tcttcaatag
     3301 cgtcgcctac agtctggctc tgcggcggca ggtcgacgtc ttctgtcccc acatggtgcc
     3361 ccggtacgcg ctgctggtag ccccgtatcg tcttctcttc cgcatcccca cggtgatgtg
     3421 gtatgcccat aactcggcgg acctcaagct gaagtccgcc aacctgtgcg tggaccgctt
     3481 cgtcacagta accagggaca gcttccccct gggatcgaag aagtgcagcg tggtggggca
     3541 cggcgtcgac acgagccttt tccggccggc accggagagc actgccacca tcgacggcaa
     3601 ggttcgcttc ctcagcctct gccgcatctc gccacgcaag gagttggaca ccttcgtgtc
     3661 cgccgcggat ctggtggtca atcgttgggg gcagacgaag gtccagttca tcattgccgg
     3721 cggccccctg gccccggagg atcacaccta ccttcagcac ctgaaggatc gggtgaagga
     3781 actgaagctg gaagagtact tcctgttcac cggcggcatc ccgcattcgg agacggtgca
     3841 ccggcttcag agctgcgatt actttgtcaa catgcacgtg gagggtggac tgggaaaagc
     3901 agtgctggag gctatgagct gcggcctgcc ggccttcgtc tccaccccca cttactatga
     3961 gcacttcccg gattacggga aacagttcct cttcgcacca cgagaccccg tgagccttgc
     4021 cgagaggatg aaggcgacgc tggagatgtc tgcggcggat cgaattcgga tcggggccga
     4081 cctgcgcgac tgggtgtgca gggaacacga tcttaagctg caaatgtcga ggatagcacg
     4141 ggtgatggaa gaggtgaagg cggggaggcg accatctacg ttcgagtaat tctcagcgaa
     4201 aaggcggcct ccgaaaagaa gacccgctac accgtgagca cacttctctc aaccctgggg
     4261 atcagccacg agttcgtgcg ggaaggggag atagatggct cggagcggtt ggccggccgc
     4321 attcacctgg tgatcggcga agccgacggc aacctgatga actatctgtg cagggaagcc
     4381 gccgtggtgg tgctcccaga gccggacttc gtgccgtcgg cggtcactca gaacccagca
     4441 cccagcactc agcacccagc actggaatat gatccggaca cgttccgcct gatctcccgg
     4501 ccaacacgca acggatacgt ggtcgggatc gccgcggacc tggttgggct ggccttcaga
     4561 ttgcctgctc gcggcgagga gtacgcggtc cccactcgtg acacccacga ccggttccaa
     4621 tgggattaca gttccctcag caagagctat cgtggcccgc ggcccctggt aagcgagtac
     4681 agccacctgc ttctggactg tttcctggag tgcagcaagc acaccaagca gccgatgatc
     4741 cggaaggagt tctggccaaa cggcaagcca atggccgggt gcctcaccca cgacgtggat
     4801 gtagtgaagc ggggcaagct ggggcacggg gtcgccgtga gggacgtgaa gggtgcacta
     4861 tccagcctca tgactggacg actcggccgg gccgcgggcc agatctcaac catcgcaacc
     4921 acagccacct ccagcaagga tccctactgg accttcgacc gcatcgcggc aatggaggag
     4981 gcccacgact tccggtcgac ctattacttc atggcagata accggcaccc ggaggacgca
     5041 gtctacaccc tggcagatcc ctctatctcg gacctgatgg cgaagctgga ggggaacggc
     5101 tgcgagatcg gcctccacgg aagctacgcc tcctatacag acgccgagat gctgagttcg
     5161 ctgaagacag aactggagca gaagctgggg catgcagtga tcgggcatcg gaaccacttc
     5221 ctccgcttcc gggttcccga ctcctggaag gcccaggaag ctgcagggtt cacctacgac
     5281 gccacactgg ggtttgccga tcacgagggt ttccggggag ggcacgcctt ccccttcgtc
     5341 ccctacgatc tggcgacgga ccgaccaatt ggactggtgg aagtcccgct ggcggtcatg
     5401 gaggtaagcc tgtcaaagta caggaagctg cgtggcgaag acgctgcggg ggctatccag
     5461 acagttctcc gacaggcaac ggccgtgggt gggcttgcca cgctactgtg gcacaacgac
     5521 agcttcttcg accccgaatc cccgggcgtc ggggctctct acgagcgctc cctggattgg
     5581 cttgccgaag agaatgccca cgtggcaccc gtccaggaga tcgtcgcctg gtggaaatcg
     5641 cgcgaggctg tacgactcat tccgctggaa gacggtcgca acggttggag aatggaagcc
     5701 cctgaggaga tcgatggact ggtactgcgc atcggtctgc ccgatgccgg ttcctcgctc
     5761 cgagtgcacg ggcaagtgcc gctggcgctg cgacgcgacg gcccggatca ccttctggag
     5821 tatgggccgc tgccggcagg attctccatg gacatcgagt accggtgaga ctcatctacc
     5881 tggccaacaa tcgcatcccg agcgagaagg ccaacagcct tcagatcatg cagatgtgcc
     5941 gggccttcca gcaggagggg gccgaggtca ggctggtggt gccccatcgg atgcagccga
     6001 aggtcatgcg cggagtgact gatccatttg cctactacgg gctgccggat cgtttcccca
     6061 tcgtgcacct gccctgcgcc gataccctgg agatcgcacc cggccggctg cagccggcgg
     6121 ccttcgccct tcagtcctcg accttctgcg tctccgtggc cggatacctg gtcggccaca
     6181 aggccgatct ctactactct cgcgaccccc tgtccaccct gctgctgggt cgcgcgcccg
     6241 agtcggtgcg ctccaggtcg gtgtacgagg cccacacctt tccaaaaccc ggccccaggc
     6301 agagggctca cgtcgactcc atagcccgga tgcgcggcct ggtgtgcata acccgggggc
     6361 tggcaaacga gtatgaagcg ctggggatac cccgtgcgag gatactggtc gcccccgatg
     6421 cggtagagtt ggagcgtttc gcctcaatgc cggatcgcga cgaggcacgg cgagagctgg
     6481 ggatcccgac ggaggcccga gtggtgtgct acacgggaca cctgtacgca tggaaagggg
     6541 cgcacaccct cgcgctggca tcgcgccacc tgcccatgga tcacctgacc tacatcgtcg
     6601 gggggactgc cgaagacctc tcgcgtttcg tccaattcct ggctgaggaa gcgttggaca
     6661 gggtgaaggc aacggggcat gtccctcccg acatggtacc gacctacctg gcggcggccg
     6721 acgtcgtcgt gctgcccaac tccggccaat cggccgcctc gtcccgctac acttcgccga
     6781 tgaagctgtt cgagtacatg gccgcccgac gaccgatagt cgcctcgcgg ctgccggcga
     6841 ttgaggaggt gctgcgccac ggggatacgg gttggctggt ggatgccgac gatccggagg
     6901 cgctcgccgc cggcatcaag gtgatgacct cctcctctga gttgatgcgg gggttggcga
     6961 gccgggcgta cgcggaggtg caacagtact cctggaaaag ccgggctaaa tccatcatgg
     7021 acttcttctc ggaggattga gtggtgtcgg gcagtcccac ggcgcagagc agagcaggaa
     7081 gaactgacag cgaggcctcg ggcgcgaaag caccggccat ggtcgcgtgg aacgtcctgg
     7141 tggccgcctc cacggctttg gccggatgcc tggtcttgct gggcagggcg ttaccacccc
     7201 aggccaccct ctggtggctc tcgctctatg cgctgatctt cgcgctaagc atggtgtggc
     7261 tgtcgttcta cgcaaatcgc gtccctaaca gatatggggc aactcgccat ctgctcctgt
     7321 tggaaacgcc tatcctactc ctatcactgg ggggaagcct ggcgctttac ctttccgacg
     7381 acctcaacca tcagctcaac tacttccttc agcccacgcc cgccgtggtg ctgctgctgg
     7441 ccgttctgtc caccgctatc gcgcaggagg cattgctcga caccagcagc gtggggctga
     7501 gaccacggct actcgccagt ctcgccaggg ccaggaggtg gcttctcccc acctccctgg
     7561 tgctcatcgg cctgcttcag ggctcgtcct acctctggat cgtgggcaac gacttcatac
     7621 gctactgggc ggtcgccgat gccacccgct cctggatggg ctatccggcc acggcacatc
     7681 tgcccagcta cgtcgagggg gggatgtacc cctactccat cgagttgccg gccttcccga
     7741 tgctgctgat gatctctttc atgctggcgg gacacgacac cctggcggcc cacctcccgg
     7801 ccctggcggc caactgcgtt ctgccgccga tcgtctacgc ctttttcagg agagccggct
     7861 tcggacggac cctctcctac gcggccgcct gctgcatggt gctgttcccg ttcctcaggc
     7921 tgtacaccct taatgcaccg gttccggacg ccgtcttcgt ggcactcctc accttcaccg
     7981 gctacctttt cctcgggtta atcgggacga accgacgggg cagcggcact cttgcagatt
     8041 gggtcgggcg gctcaacgac agctcacccg gctcggcccc caacccccaa ccgccaaccc
     8101 tcaaccccca gcggtgggcc gccttcgggt tggcggcagg cctgaccacg ctgaccaggc
     8161 ccgaggggtt gctgttcgtc ggatgcatgt tcctcgccct gatcgcgaac ttgctcaagc
     8221 cacgactcta cctggcaggg gccatcttcc ttgccggtgt ggtacctttc tcgctgttga
     8281 tgctctccac cttcggtatc ccctgggctc gcaacgcgga ctcgtccttc ggtttggaga
     8341 acatcgttgg caacttgaac tggatcaacg ggatgaccct gcccttcttc gccgatccat
     8401 tccggctgcc ctatccggtc ttcatcgcgc tggtcgcgct gatggtgctg gccatggggc
     8461 tgggcaccct ctggatcgcc acgaaatctt ggcagatggc cgcgctgccc gcggctgctg
     8521 ccttccacat cttcggggtg ttcacagtgg accccagggt gagcggcgtc ga
//
LOCUS       DATALS010000113         3948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_236053, whole genome shotgun sequence.
ACCESSION   DATALS010000113 DATALS010000000
VERSION     DATALS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236053"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VHS06_02395"
     CDS             <1..208
                     /locus_tag="VHS06_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987001.1"
                     /translation="LLLAEIERGAADEVEQAEVGRAADQADLVIVDEEGAVGEEVEGG
                     EGEGEGGVVITDYSVTGGRVVGGW"
     gene            218..2530
                     /locus_tag="VHS06_02400"
     CDS             218..2530
                     /locus_tag="VHS06_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX2987002.1"
                     /translation="MLFIHNNQVGLIRGPANLRLLDFIRRTPLDLGKQEISVLLKHLA
                     RIPGAHELIWPADLGIAREIPPCNPILICALHTTRSQWISEDMVTAELEFAYGQTRVT
                     SANPSSAFYDESRRVVVMRNLPAEAAFRQRLDDLEFRFRFIATGQRKLMLKADRFREA
                     ALILATEGWQVEGEGIPRYIPPGELKLQINSGIDWLDVDGEIQFGDQKVKLPALLQAV
                     RRGDRFIELPGGRKGLLPQDQLRPLASIAQDKPDSDTLRFTPSQAALVDAICSTTPTA
                     LTDDRFRSLRNALQQGATVQPLDPPVGFVGELRHYQKEALGWFTFLRDQSFGGCLADD
                     MGLGKTVQVLAMLEGRRQDRHEGKLRGPRPSLVVAPRSLIFNWIQEAAKFTPLLRVLD
                     YSGASRSLDQIPEADLVLTTYGTLRRDAADLARTRFDCVILDEAQAIKNASTATAKSV
                     RVLDAQHRLALSGTPVENHLGELISLFEFLNPGMLCSTHLAEDAVSGKAMDPDTARLL
                     ARAVRPFILRRTKQQVAPELPERTEQTLLVELERAQRHEYNELRNYYQRTLLGEVRMH
                     GLSRMRFQVLEALLRLRQAACHPGLIEPSRINDSSAKLEAILEEISEVIEEGHKALVF
                     SQFVGMLTILRKQLDARGITYEYLDGATADRQTHVERFQSDPACPLFLISLKAGGVGL
                     NLTAAEYVFLLDPWWNPAVEAQAIDRAHRIGQTRQVFAYRLIAKDTVEEKVLDLQNRK
                     RALADALIGTPDDQAPTESMSAADVELLLS"
     gene            complement(2582..2956)
                     /locus_tag="VHS06_02405"
     CDS             complement(2582..2956)
                     /locus_tag="VHS06_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017654293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX2987003.1"
                     /translation="MMGPRVPQDIYERTEDYALRAIKLFRYLKRKRDEDGVIIGRQYL
                     RAATSIGANMVEADSAESRRDFIHKCNVALKEAKECRYWLRLLMRAEIVTPSRLARLV
                     DATQQITLVLASIVIKAKRKRA"
     gene            complement(3011..>3948)
                     /locus_tag="VHS06_02410"
     CDS             complement(3011..>3948)
                     /locus_tag="VHS06_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX2987004.1"
                     /translation="TVSMAMATKLILDTDIGDDVDDALCLGLILGCPEVELRGVTTVF
                     GNVAARARQARTILKVAGEAFARVPVSAGCGGSMASRPLHNVRAYLEDHLPNQDASCL
                     PEAELPEGDPRHGVDLLLDELKGDVVPVTIGAMTNLAMAIVKDWRVIRRIPRILSMAG
                     EFRAPMAEWNIRCDPEAAHIVLGSGVPMDIIPWTIGMQVSFRQEHLDRLSRSRRPLAQ
                     RLAMAIQCWQMNWSDNGKKARPMPHLFDPMTIATLIWPELCTWKRGWVKVELTGTETY
                     GYTTFREDENGPHRVAWDAKVEESLGFWFERIEQV"
BASE COUNT          710 a         1479 c         1043 g          716 t
ORIGIN      
        1 tctcctgctt gccgagatcg agcggggtgc ggcggatgaa gtcgagcagg cggaggttgg
       61 ccgggccgcg gatcaagccg acttggttat tgtggatgaa gagggtgccg tcggcgagga
      121 ggtggaggga ggagagggag agggagaggg aggagtagtg attactgatt attccgtaac
      181 agggggcagg gtcgttggtg gatggtgatt ggatggcatg ctcttcatcc acaataacca
      241 agtcggcttg atccgcggcc cggccaacct ccgcctgctc gacttcatcc gccgcacccc
      301 gctcgatctc ggcaagcagg agatctccgt cctccttaag catctcgcga ggatccccgg
      361 agcgcatgaa ctgatctggc ccgccgacct tggcatcgcc cgcgaaatcc cgccctgcaa
      421 tcccatcctc atctgcgccc tgcacaccac ccgctcccag tggatcagcg aggacatggt
      481 caccgccgag ctcgagttcg cctacggcca gacgcgcgtc acctccgcaa atccctccag
      541 cgcgttctac gacgagtctc gccgggtcgt cgtcatgcgc aatctccccg ccgaagccgc
      601 cttccgccag cgcctggatg atctggaatt ccgcttccgt ttcatcgcca ccggccagcg
      661 taagctgatg ctcaaggctg accgattccg cgaagccgcc ctgatcctcg ccacggaagg
      721 ctggcaggtc gaaggcgaag gcatcccccg ctacatcccg ccaggcgaac tcaaactgca
      781 gatcaactcc ggcatcgact ggctcgatgt cgacggcgag atccagttcg gcgaccagaa
      841 ggtcaagctc ccggcactgc tccaggccgt ccgccgcggc gaccgcttca tcgaactgcc
      901 cggcggccgc aaagggctgc tgccgcaaga ccaactccgc cccctggctt ccatcgccca
      961 agacaagccc gactccgaca ccctccgctt cactccctcc caggccgctc tggttgacgc
     1021 gatctgctcc accacgccca cggcactcac cgacgatcgc ttcagatccc tccgcaacgc
     1081 attgcagcag ggcgctacgg tccagccact cgatccgccg gtcggtttcg tcggcgagct
     1141 gcgccactac cagaaagaag ccctgggctg gttcaccttc ctgcgcgacc agtccttcgg
     1201 cggctgcctc gccgatgaca tgggcctggg caagacggtc caggtgctcg cgatgctgga
     1261 agggcgccgc caggatcgcc acgagggcaa gctccgcggc ccgcgtccgt cgctggtcgt
     1321 cgctccgcga tcactgatct tcaactggat ccaggaagcc gcgaagttca cgccgctgct
     1381 tcgtgtgctc gattacagcg gcgcttcccg cagcctcgat cagatccccg aggccgatct
     1441 cgtcctcact acctacggta ccctccgccg cgacgcggcc gacctcgccc gcacccgctt
     1501 cgactgcgtc atcctcgatg aagcccaggc catcaagaac gcttccaccg ccaccgctaa
     1561 gtccgtccgc gtgctcgatg cccagcatcg cctggcgctc tccggcacgc cggtggaaaa
     1621 tcacctgggc gaactgatca gcctcttcga gttcctcaat cccggcatgc tctgctccac
     1681 gcacctggcc gaggacgccg tctccggcaa ggcgatggac cccgatactg ccaggttgct
     1741 ggcccgcgcc gtccgcccct tcatcctccg ccgcaccaag cagcaggtcg cccccgaact
     1801 ccccgaacgc accgagcaga ccctgcttgt cgaactcgaa cgcgcccagc gtcacgaata
     1861 caacgagctc cgcaactact atcagcgcac gctcctgggc gaagtccgca tgcacgggct
     1921 ctcccgcatg cgcttccagg tgctcgaagc gctgttgcgt ctccgccagg ccgcctgtca
     1981 tcccggcctg atcgagccct cgcggatcaa cgattccagc gccaagctcg aagccatcct
     2041 cgaagagatc tccgaggtca ttgaggaagg ccacaaggcc ctggtcttct cccagttcgt
     2101 cggcatgctc accatcctgc gcaaacagct cgatgcccgc ggcatcacct atgagtatct
     2161 cgacggcgcc accgccgacc gccagaccca cgtcgagcgg ttccagagcg atccagcctg
     2221 cccgctcttt ctgatcagcc tcaaggccgg cggcgtcggc ctgaatctca ccgcggccga
     2281 gtatgtcttc ctcctggacc cctggtggaa ccccgccgtc gaggcccagg ccatcgaccg
     2341 cgcccaccgc atcggccaga cccgccaggt cttcgcctac cgcctgatcg ccaaggacac
     2401 cgtcgaggaa aaggtcctcg acctccagaa ccgcaaacgc gccctcgccg acgccctgat
     2461 cggcaccccc gacgaccaag cccccaccga gtccatgagc gccgccgacg tggaactcct
     2521 gctctcctga gagcaatgca aaatgacaaa tggcaaatgc agaatggaag acgcccggcc
     2581 ctcaggcgcg cttgcgtttt gccttgatta cgatgctggc gagaaccaac gtgatctgct
     2641 gtgtcgcgtc cactagtctg gcaaggcgac ttggcgtcac gatctctgct cgcatcagca
     2701 gacgcagcca atatcgacac tccttcgctt ccttcagagc gacattgcac ttgtggatga
     2761 agtctctccg cgattcggcc gaatctgcct ccaccatgtt cgcaccgatg gaggtcgcgg
     2821 cccgcaggta ttgccggccg atgatcacgc cgtcctcgtc tcgctttcgt ttgaggtagc
     2881 ggaacagctt gatcgccctg agcgcatagt cttcggtgcg ctcgtagata tcctgcggaa
     2941 ctcgcggtcc catcattgct tcttcctcat tttgcatttg ccatttgtca ttggtcattc
     3001 cgtctgctgg tcagacttgc tcaatccgct caaaccagaa ccccaggctc tcctccacct
     3061 tcgcatccca cgccacccgg tgcggcccgt tctcgtcctc ccgaaatgtc gtatacccgt
     3121 acgtctctgt ccccgtcagc tccaccttca cccagcctcg cttccacgtg cacaactccg
     3181 gccagatcag cgtcgcgatc gtcatcgggt caaacagatg cggcatcggc ctggccttct
     3241 tgccattgtc gctccagttc atctgccagc actgaatcgc catggccagc cgctgtgcca
     3301 gcggccgtct gctcctgctt aaccgatcca ggtgctcctg ccggaaactc acctgcatcc
     3361 cgatcgtcca cgggatgatg tccatcggaa cccccgagcc aagcacgatg tgcgctgcct
     3421 ccggatcgca acgaatgttc cactccgcca tcggtgctcg gaactcaccg gccatgctca
     3481 ggatcctcgg gatgcgccgg atcacccgcc aatccttcac aatcgccatc gccaggttcg
     3541 tcatcgcccc aatcgtcacc ggcacgacat cgccctttag ctcatccagc agcaagtcca
     3601 ccccatgccg cggatcgccc tcaggcagct ccgcctccgg cagacagctc gcatcctggt
     3661 tcggcagatg atcctccaga tacgcccgga cattgtgcag cggccgggat gccatcgacc
     3721 cgccgcaccc ggccgacacc ggaaccctcg cgaacgcttc ccctgcgacc ttcagaatcg
     3781 tccgcgcctg cctcgcccgc gccgccacat tgccaaacac ggtcgtcacc ccgcgcagct
     3841 ccacctccgg gcaccccagg atcagcccca ggcacagcgc atcatccaca tcatccccga
     3901 tatccgtatc caggatcagc ttcgttgcca tggccatgct cacagtcc
//
LOCUS       DATALS010000114         7766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_49991,
            whole genome shotgun sequence.
ACCESSION   DATALS010000114 DATALS010000000
VERSION     DATALS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49991"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VHS06_02415"
     CDS             <1..747
                     /locus_tag="VHS06_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX2987005.1"
                     /translation="ASGDKKVDDVLDLCRGMADVARRFGAVVVGGDTVSSPLTTVSIT
                     LLGEAVGTRRPDGSLPILSRFAARPGDVLAVTGRLGSSGGGLELLLKGGAPIPDKLAP
                     LLEAHRRPMPRVREGRALAEAGVRCGMDLSDGLAGDLTRVCRASKVSALVDVERLPLD
                     PLLREVFGSRAVDLALTGGEDYELLCAAPPEIIERAQKLLDSMGGALTPVGRVIEAGT
                     EPPQVKLTDSTGRSYAPTRSGWEHFSSNGN"
     gene            737..1255
                     /gene="tsaE"
                     /locus_tag="VHS06_02420"
     CDS             737..1255
                     /gene="tsaE"
                     /locus_tag="VHS06_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX2987006.1"
                     /translation="MATEPVSRHVGFVSRTPAQTKAFGAQLGRVLQPGDLILLQGPFG
                     AGKTTLVQGLGAGLKTGGRVTSPSFTLINEYQGRLKLYHVDLFRLESLDMELEQAIED
                     CESGEGVTVIEWPDLLPHDLKHGALRIDLEVMGDEERGLLIHAEGTRWGVGELTEMLR
                     AALQESEAVADE"
     gene            1248..1913
                     /gene="tsaB"
                     /locus_tag="VHS06_02425"
     CDS             1248..1913
                     /gene="tsaB"
                     /locus_tag="VHS06_02425"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX2987007.1"
                     /translation="MSSVVLALDTSTAIASVALYDGAVSGEVTWRSGRNHSVELMVQA
                     QGLMKLRGVDPSQLVAVAVATGPGSYTGLRVGLSAAKGLCLALGLPMVGVCSLDILAE
                     AQRDSCLPIRPLLDAGRHRFATALYRMNGAGLERVSPIEGLRLPEVLDRVKERTLICG
                     DVAELSAVDLGSRAALFDVAGPASSLRRAGFLAEIGWKRYHSGDVQEASEVDAFYLSK
                     EEP"
     gene            1954..2640
                     /gene="rimI"
                     /locus_tag="VHS06_02430"
     CDS             1954..2640
                     /gene="rimI"
                     /locus_tag="VHS06_02430"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX2987008.1"
                     /translation="MQIEDIPAVMEVERQSFTVPWPSHAYKRELKENRLARYIVARRT
                     FPGPDIFGNRQAEGESDGVVTHSTTRRGLLGWISDLMGGNGQPSASVGERAQRVVGYA
                     GLWLMVDEAHITSIAVASAYRGLGIGELLFLSLVDMSRQIGAQYVTLEVRVSNILAQN
                     LYRKFGFKETGVRRRYYSDNNEDALIMWSDPLDSPDFQHMVEQHRQALMARLKSSQTV
                     SELRARGVQA"
     gene            2787..5702
                     /gene="polA"
                     /locus_tag="VHS06_02435"
     CDS             2787..5702
                     /gene="polA"
                     /locus_tag="VHS06_02435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX2987009.1"
                     /translation="MAGKIALIDGNALVHRAFHALPPLTSPKGELVNATYGVATMLLK
                     VLQEMHPEYAAAAFDTPVPTFRHVAYQEYKGQRGPAPEGLHEQFGRVYELLDALGIPT
                     YRADGFEADDLLGTMARQAREAGLEVVIVTGDTDALQLVAPGVTVLTSRKGFTDTVLY
                     DEEGVKERYGVEPPKLVELKALKGDASDNIPGVPGVGEKTASKLVAEYGDLEGIYANL
                     EKITGKLKQQLAENREQAFFSRHLGKIVTEAPVTLEPEKGRTGRYNRPRLVELFRELG
                     FKSLVDRIPKEEQPAGTGSVGGSGVPNGNGEQLSLFSAEAPTPAGEQVAVAGPPAGEV
                     EAETGVDAGSMGADRDYRVVSTQEELDALVALLREAPIFSFDLETTGVDALKADLVGL
                     SFSVQPGQAFYVPVGHRPAGELGLGLLGSMATTTEGAEDEPAQLPLKLVLERLKPVLE
                     DETAKKCGHNGKYDMLSLARYGIYVRGLAFDSMVAAYLLESTQRTLGLKDLAWSRLNV
                     EMESYQSLAGKGKTAVTLDQLPIRRVADYSCADADMTLRLMHSLKPELEAKGVEELFE
                     RVEMPLVPVLLDMECEGVSLDVPYLQKLSTELYQRIRELETAICDAAGHSFNIASTQQ
                     LAHVLFKELLLPATRRTKTGYSTDADVLEELKGTHPIVELVLEHRQMTKLKSTYVDAL
                     PLLVNPRTGRVHTSFNQTVASTGRLSSSDPNLQNIPIRTEMGRRVRRAFVARDESWTL
                     LSADYSQIELRVLAHMTGDPVLVDAFQRGEDIHAATAALVYGIPLSEVTPTERRIAKT
                     VNFGVLYGMSDFGLARDTGLTRKEAGAFIDSYFQRYASVTQLFDRIKAQAEETGYVST
                     MLGRRRYIPEIRQKHRGLKQAAERMAINMPIQGTAADIIKIAMINLRRRMSEMGLQAR
                     MILQVHDELLFDCPRSEVELLAPEVKRIMESAMSLQVPLVVDLKLGHNWDEMGRYPL"
     gene            5702..6628
                     /locus_tag="VHS06_02440"
     CDS             5702..6628
                     /locus_tag="VHS06_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEX2987010.1"
                     /translation="MASWSDGADVRFQIAPRFLNLDYTLDCGQAFRWKRNGGGWWCGV
                     VRGQAVRLRRDGGEVVGQLYPGREDAEPFLASYFRLDVELETLYREFAAADEWIAAAV
                     ESYQGLRVLNQEPQETLLSYICSTANSVPRISKAVAEMSRRWGRLIGILDGEEFHAFP
                     TAEALAAAPEDALWRDCGLGWRGTNLKRLAGDLVPMADGWPEQLRGLPYREAKARLME
                     LRGIGAKIADCVCLFSLEKDEAVPVDTHIWALAKEIFSCEISNKTLTPATYETVAKLY
                     LERYGSFAGWAQEYLYLHRRASQGRVSSARSE"
     gene            complement(6829..7083)
                     /locus_tag="VHS06_02445"
     CDS             complement(6829..7083)
                     /locus_tag="VHS06_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987011.1"
                     /translation="MGIAWAAANLANFNLVLGISNEQNRAAVVAWVSVYQAPADFAAP
                     LIGGFLADRIDLPALFIISVLIRLAAWGSFTTRLAKGDER"
     gene            7137..7763
                     /locus_tag="VHS06_02450"
     CDS             7137..7763
                     /locus_tag="VHS06_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2987012.1"
                     /translation="MGLSPASVGNEDHTRLQESLPGWKMPAVRNYAVYGTLLILGAGI
                     STPFFPVYFISRLQGSPETAAFMIATANAITMLAQGLWGRVIDRKGLAPVAHGSLLVI
                     ALSPLLWLFAQQPVHAAPIWLAMGIAWAAANLANFNLVLGISNEQNRAAVVAWVSVYQ
                     APADFAAPLIGGFLADRIDLPALFIISVLIRLAAWGSFASRLANGDER"
BASE COUNT         1418 a         2260 c         2674 g         1414 t
ORIGIN      
        1 gccagcggcg acaaaaaggt cgacgacgtg ctcgacctct gccgcggaat ggccgacgtg
       61 gcccgccgat ttggggccgt ggtggtcgga ggcgacaccg tctcgtcacc cctcactacc
      121 gtcagcatta ccctgttggg cgaagccgtc ggcaccagaa ggccggatgg tagcctgccg
      181 atcctcagtc gtttcgcagc gcgccctggt gacgtcctgg ccgttaccgg ccggctgggc
      241 tcttccggag gagggctgga gctgctgttg aagggcggcg ccccaatccc tgacaagctt
      301 gcacccctgc tggaggccca tcgacgtcca atgcccaggg tacgtgaggg gcgggcgctg
      361 gcagaggccg gcgtgcgatg cgggatggac ctgagcgatg gcttggcagg cgacctcacc
      421 cgtgtctgcc gtgcaagtaa ggtgtccgct ctggtggatg ttgagagact cccgcttgat
      481 cctctgcttc gagaggtctt cggctcgcgt gccgtggatc ttgccctcac cgggggcgag
      541 gactatgagt tgctctgcgc cgcaccgcca gaaatcatag aaagggccca gaaattgctg
      601 gacagtatgg gcggtgcact gacgccggtg ggaagggtga tcgaagctgg caccgagccg
      661 cctcaggtga agctgacaga ctccacgggc agaagctacg cgccgacacg atctggatgg
      721 gagcatttct cctcaaatgg caactgagcc tgtttcccga cacgtcggat ttgtaagtcg
      781 cacgcccgcc cagaccaaag ccttcggcgc gcaactggga cgggtgctcc aaccaggtga
      841 ccttatactg ctgcaaggcc ccttcggcgc gggcaagaca accctggtcc agggactggg
      901 ggccgggctg aaaacgggag gacgggtcac cagtccctct tttactctca tcaacgagta
      961 tcagggcagg ctgaagctgt atcacgtgga cctcttccgt ctcgagtcgc tggacatgga
     1021 gttggagcag gctatcgagg actgcgagag tggggagggc gtgacggtga tcgagtggcc
     1081 tgaccttctc ccccatgatt tgaagcacgg tgccctgcgg atcgatctgg aagtcatggg
     1141 ggatgaagag cgtggcttgc tcatccacgc cgaaggtacc agatggggag tcggcgagtt
     1201 gacggagatg ttgagggcag cgcttcagga gtcggaggcc gttgcggatg agtagcgtcg
     1261 ttctggcgct ggatacttcc accgccatcg ccagcgtggc tctgtacgac ggggcggttt
     1321 cgggggaggt aacctggagg tcgggacgga atcactcggt cgagttgatg gtccaggctc
     1381 aggggttgat gaagctgcgg ggagtggatc cctcgcagtt ggtggcagtt gccgtggcta
     1441 caggaccggg ctcatatacc ggtctcaggg taggactttc ggcggccaaa ggactctgcc
     1501 tggccctggg cttgcccatg gtgggggtgt gttcgctgga catccttgcc gaggctcagc
     1561 gggacagttg cctgccgata cgtccgttac tggacgcagg tcgacaccgc tttgcgacgg
     1621 ctctctaccg gatgaacggt gcagggttgg agagggtgag ccctatcgag gggctgcggc
     1681 tgcccgaggt gctggatcga gtgaaagaac gaaccttgat ctgcggcgac gtggctgagt
     1741 tgtcggccgt cgatcttggg agccgcgcag cgttgttcga tgtggctggc ccggcctctt
     1801 ccttgcggag ggccggattc ttggcggaga ttggctggaa gcgctaccat agcggcgacg
     1861 tccaggaagc gtcggaagtg gatgccttct acttgagcaa ggaggaaccg tagcattaga
     1921 gccgaagagc agcttccctt tgtaatcgag ccgatgcaga ttgaggacat cccggccgtg
     1981 atggaggtgg agcggcaatc cttcactgtg ccgtggccta gccacgcata caagcgcgaa
     2041 ctgaaggaga accggcttgc ccgctacatc gtcgcccgcc gcacattccc tggccccgat
     2101 atcttcggga accggcaggc ggagggcgag tccgacggcg tggtgacaca ctccacgact
     2161 cggcgcggcc tgctgggctg gatctccgat ctcatgggcg gcaacggtca gcccagcgct
     2221 tctgtgggcg agagggcgca gcgggtggtc ggctatgccg gactctggct catggtggac
     2281 gaagcgcata tcacctctat cgccgttgcc tcagcttacc ggggattggg gatcggcgag
     2341 cttctcttcc tctccttggt ggatatgtcc cgtcagatcg gcgcgcagta cgtgacgctc
     2401 gaggtcaggg tgagcaacat cctggcgcag aacctctatc ggaagttcgg cttcaaggaa
     2461 accggtgttc gccgcaggta ctacagtgac aacaacgagg atgcattgat catgtggtcg
     2521 gacccgttgg actcgcccga cttccagcac atggtggagc agcatcgaca ggcgctgatg
     2581 gcccggctca agtcctcaca aaccgtctcc gagctacggg caaggggtgt tcaggcatag
     2641 tagaggcccc tcctatggta caatttgagt gacccctaaa ggggcgaccg gctatcggtc
     2701 gcccctgcgt aattatcgag actgaccctt gtaggggcga cgcacgcgtc gccccattca
     2761 atcctcgagg gcagcaagga ggagccgtgg cagggaagat cgcactaatc gatgggaacg
     2821 ccctggttca tcgggctttc cacgccctcc ctccacttac gagcccgaaa ggcgaactgg
     2881 tcaacgctac ctacggcgtt gccaccatgc tgctgaaggt gctccaggag atgcacccgg
     2941 agtacgccgc ggccgccttc gatacccccg tccccacctt ccgccacgta gcctaccagg
     3001 agtacaaggg tcagcgaggt cccgcccccg agggactcca cgagcaattc ggtcgggttt
     3061 acgagctgct ggacgccctg ggaatcccca cctatcgggc agacggcttc gaggcggacg
     3121 acctgttggg cactatggcg cggcaggcgc gcgaggcggg gctggaggtg gtgatagtca
     3181 ccggcgacac tgatgccttg cagcttgttg cgccgggtgt gacggtcctc acctcgcgga
     3241 aggggttcac ggacactgtc ctgtacgatg aagagggagt gaaagagcgg tacggcgtcg
     3301 agcccccgaa gttagtagag ttgaaggcgc tgaaggggga tgcctccgac aatattcccg
     3361 gagttcccgg agtgggcgag aagacggcct ccaagctggt tgccgagtac ggcgacctgg
     3421 aggggattta cgccaacctt gagaagatca ccggcaagtt gaagcagcag ctggcagaga
     3481 accgagagca ggcgttcttc tctcgacatc tggggaagat cgtgaccgag gcgcccgtga
     3541 cgctggagcc agagaagggc cgcaccggta gatataaccg acccaggctg gtagagcttt
     3601 tcagggagtt ggggttcaag agcctcgtcg accggatacc gaaggaagag cagcctgctg
     3661 ggacaggctc cgtaggcggt agcggtgttc cgaacgggaa tggcgagcag ctatcgctct
     3721 tctctgcgga ggccccaacg cccgccggag agcaggttgc cgtggccggg ccaccggccg
     3781 gtgaggtgga ggcggagact ggcgtcgacg ccggttcgat gggggcggac agggactacc
     3841 gcgtcgtttc cactcaggag gagttggatg cgctggtggc actgcttcgc gaggccccca
     3901 tcttctcctt cgatctggag accaccggcg tggatgcgct gaaggcggat ctggtgggac
     3961 tatccttctc tgtgcagccc gggcaggcgt tctacgtgcc ggtggggcac cgtccagccg
     4021 gcgagttggg tcttgggttg ttgggctcca tggccaccac gaccgaaggc gccgaggacg
     4081 agcctgcgca gcttccgttg aagctggtgc tggagcggtt gaagccggtt ctggaagacg
     4141 aaaccgcaaa gaagtgtggc cacaacggca agtacgacat gctgtcgctg gcccgctacg
     4201 ggatctacgt tcgcggactc gccttcgaca gcatggtcgc agcctatctg ctggagagca
     4261 cgcaacggac cctcgggctg aaggacctgg cctggtcccg gttgaacgtg gagatggaga
     4321 gctaccagtc gctggcgggg aaggggaaga cggcagtcac cttggaccag ctgccgatcc
     4381 ggcgagtcgc cgactacagc tgtgccgatg ccgacatgac gcttcggctg atgcacagcc
     4441 tgaagccgga gctggaggcc aagggtgtag aggaactctt cgagcgggtc gagatgcctc
     4501 tggtgccggt gctgctggac atggagtgcg agggagtctc cctggacgtg ccctacctcc
     4561 agaagctgag cacagagctg taccagcgca tccgcgagct ggagacggca atctgcgatg
     4621 ctgccggcca cagctttaac atcgcttcca cccagcagtt ggcgcacgtg ctgttcaagg
     4681 agttgctgct cccagccacc aggcgcacca agaccggcta ctccaccgat gcggacgtcc
     4741 tggaggagtt gaagggcact caccccatag tggaactggt gctggaacac cggcagatga
     4801 cgaagctgaa gtccacctac gtggatgccc tgccgttgtt ggtgaaccct cgcaccggga
     4861 gggttcacac ctccttcaac cagacggtgg cctccaccgg ccggctctca tcgagcgacc
     4921 cgaatctgca gaacatcccg atccggacgg agatggggcg acgggtgcgg cgggccttcg
     4981 tagcgcggga cgaatcctgg acgctgctct ccgcggacta cagccagatc gagctgcggg
     5041 tgctggccca catgaccggc gacccggtgc tggtggacgc cttccagcga ggggaggata
     5101 tccacgcggc cacggcggcg ttggtgtacg gcattcccct ctctgaagtg acgccgaccg
     5161 agcggcggat cgccaagacg gtgaacttcg gcgtgctgta cgggatgagc gacttcggcc
     5221 tcgcgaggga caccggtctc acccgcaagg aggccggggc cttcatcgac agctacttcc
     5281 agcgctacgc ttcggtgacg cagctgttcg accgcatcaa ggcgcaggcg gaggagaccg
     5341 gctacgtgag caccatgctg ggcaggcggc gctacatccc ggagattcgc cagaagcatc
     5401 gcgggctgaa gcaggctgcc gagcggatgg ccatcaacat gccgatccag ggtaccgctg
     5461 ccgacatcat caagatagcg atgatcaacc tgcgccgccg catgtccgag atggggctgc
     5521 aggctcggat gatcctgcag gtgcacgacg agttgctgtt cgactgcccg aggtcggagg
     5581 tagagctgct ggcgccggag gtgaagcgga tcatggagtc ggccatgtcg ctgcaggtgc
     5641 cgctggtagt ggacctgaag ttgggtcaca actgggacga gatgggaagg tatccgctgt
     5701 aatggcaagc tggtcggacg gcgccgacgt gcgcttccag atcgctcccc gtttcctgaa
     5761 cctggactac acgcttgatt gcggccaggc attccgctgg aagcgcaatg ggggcggctg
     5821 gtggtgcggc gtggtgaggg ggcaggccgt tcggctgcgg cgtgacggcg gcgaggtggt
     5881 cggccagctg tatccgggca gagaggacgc ggagccgttc ctggcttcct actttcggct
     5941 ggacgtagag ttggagacgc tgtacaggga gttcgccgcc gcggacgagt ggatagccgc
     6001 ggctgtggag tcatatcaag ggcttcgggt gctgaaccag gagccgcagg agacgctact
     6061 ctcttacatc tgctccacgg ccaactcggt gccgaggatc tcgaaggccg tcgcggagat
     6121 gtcccggcga tggggcaggc ttatcggcat cctggatggt gaggagttcc acgcctttcc
     6181 gactgccgag gcgctggccg cggcgccgga ggacgcgctg tggcgggact gcgggctagg
     6241 ttggcgcgga acgaacctga agaggctggc cggtgacctt gtgccgatgg ccgatggttg
     6301 gccggagcag cttcgaggtt tgccttaccg agaggcgaag gctcggttga tggagttgcg
     6361 cgggatcggc gcgaagatcg cggattgcgt ctgcctgttc tccctggaga aggacgaggc
     6421 ggtgccggtg gacacacaca tctgggcact tgccaaggag atcttctcct gcgagatctc
     6481 caacaagacc cttactcccg ccacttacga gaccgtggcg aagctgtacc tggagcggta
     6541 tggcagcttc gcaggctggg cccaggagta cctctacctt catcggcgcg cctcccaggg
     6601 ccgggtgtcc tcggcgcgtt cggagtaggt ggtagggcag gggattctga gataatcgcc
     6661 ttggttacgc tcagcggatt tgcctgggcg acccgaaatt acttgtaggg cggggtcttg
     6721 tgccccgccc aaagcgtggt cggaaaccac caggaggtca taggcacact gtgggcggcg
     6781 gggcacaaga acccgcccta cgagacgtcg tgggtgagtc cagaccctct accgctcatc
     6841 ccccttggcc aaacgtgtcg tgaagcttcc ccaggcggca agccgaatca gcaccgagat
     6901 gatgaacagg gcggggaggt cgatccggtc cgcgaggaag cccccgatca ggggggcggc
     6961 gaaatctgcc ggcgcttgat agaccgagac ccaggccacg acagccgctc tgttctgctc
     7021 gttggagatc ccaagcacca ggttgaagtt ggctaagttt gcggccgccc aggcgatccc
     7081 catcgccaac catatgggcg ccgcgtggac cggcgctggt cgctcagggc gtcgccgtgg
     7141 gattgagtcc cgccagcgtc ggcaacgagg accatacacg attacaggag agtctgcccg
     7201 gatggaagat gccggcggtg cgcaactacg ccgtttacgg gacgctactg atcctgggag
     7261 caggcattag cacccccttc ttccctgtct acttcatctc acgcctgcag ggatcgccgg
     7321 agaccgcggc cttcatgata gcgaccgcca acgctatcac catgctggct caagggctgt
     7381 gggggagggt aatcgacaga aagggtctgg cgccggtcgc ccacggcagc ctgctggtaa
     7441 tcgcactgtc gcccttgctg tggctctttg cccaacagcc ggtccacgcc gcgcccatct
     7501 ggttggcgat ggggatcgcc tgggcggccg caaacttagc caacttcaac ctggtgcttg
     7561 ggatctccaa cgagcagaac agggcagcgg tggtggcgtg ggtatcggtc tatcaggcgc
     7621 ctgcggattt cgccgcgccc ctcatcggag gcttcctcgc ggaccggatc gacctccccg
     7681 ccctgttcat catctcggtg ctgattcggc ttgccgcctg gggaagcttc gccagtcgcc
     7741 tggcaaatgg ggatgagcgg tagagg
//
LOCUS       DATALS010000115         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_368845, whole genome shotgun sequence.
ACCESSION   DATALS010000115 DATALS010000000
VERSION     DATALS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368845"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VHS06_02455"
     CDS             <1..609
                     /locus_tag="VHS06_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEX2987013.1"
                     /translation="AVIEGNAAKVKELTQQALNGGDAPQALLDGALIPAMDVVGEKFA
                     NREFFIPEMLIAARAMQAGLGLLKPLLAGQESKSRGKVVIGTVKGDLHDIGKNLVGMM
                     LQGAGFDVVDLGADVKPAKFVETVAAQKPDFVMMSALLTTTMLSMKETIAALKEAGLR
                     DGVKVGVGGAPVTQRFADEIGADFYAADATGAVGKAKEFAAA"
     gene            complement(713..1738)
                     /locus_tag="VHS06_02460"
     CDS             complement(713..1738)
                     /locus_tag="VHS06_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX2987014.1"
                     /translation="MSAELDRMAKSLSRRQFLAASMASAAGLAMTRGNVLAAPEPRRA
                     LQTQEEYVWIGAVTAIAFWLDGQQGFHAAGNALGVKTTYLGPVEYDPVAQMNILEQLI
                     ASKPAGIMIFPADDQSLNDPLKQAMAAGIPVVVVNHDVNDPTARYGFVGPDNVNVGRI
                     GGQAAAKFMNGRGKVGFVTTTNPAHGQRVSGYKEVFAKYPDIQIVGSVDEKSDPNVAL
                     TVSTQLLQANPDLTMAIGIDATAGAAIGRALKETGLAGKVMGGAMDRDDDMLPYIKDG
                     TLTYSMAQNSVMEEWMATHYLYWLVHNTIPAFPDWRAAGAPQVPGLTDTGVTLITKDN
                     VDHFFHR"
     gene            complement(1887..2936)
                     /locus_tag="VHS06_02465"
     CDS             complement(1887..2936)
                     /locus_tag="VHS06_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2987015.1"
                     /translation="MSGETSVARESPNATVAPPHGQTGAGPSGHGLSQSMTRLIVSRE
                     MAMLALILATGIILSLTTTRFLNGPNLIAIARAFSMEALVVVGMSMLLIGGFFDLSVG
                     SVMALAGIVCASLIAKGHLPIPLAILGGLAVGAITGGINGFVVTRAKVNALIATLGMM
                     SVARGIALGYTEGRPVINLPQSFNIIGQGDLFSLPLIVLYMLVVVLSADILLRRARAL
                     RQIYYLGGSEKAARLSGINVDRVVFLTFIASGILAALAGIITTARLTSGVPTAFTGVE
                     LRIIAACVLGGCSMAGGEGTVLGAVLGLVFMAMMSNAMTLFGVSIYWEGVITGTILVV
                     AVSLDMISRRRLRTT"
BASE COUNT          559 a          929 c         1013 g          560 t
ORIGIN      
        1 gccgttatcg agggcaacgc cgccaaagtg aaggagttga cccagcaggc gctgaacggc
       61 ggggatgcgc cgcaggcgct gctggacggg gcactgatcc cagccatgga cgtggtgggc
      121 gagaagttcg ccaatcggga gttcttcatc ccagagatgc tgattgcagc gagggcgatg
      181 caggcggggc tcggtctact caagccgctg ctggcaggcc aggagagcaa gtccaggggt
      241 aaggtagtga taggcaccgt caagggagac ctgcacgaca taggcaagaa cctggtgggg
      301 atgatgctgc agggtgcggg cttcgatgtg gtggaccttg gggcagacgt gaagcctgcc
      361 aagttcgtgg agacggtggc tgcacagaag cccgacttcg tgatgatgag tgcactgctg
      421 acgacgacga tgctctccat gaaggagacg atagcggcgt tgaaggaagc ggggctgagg
      481 gacggcgtga aggtcggggt tggcggggcg ccggtgacgc aacgctttgc cgacgagata
      541 ggtgccgact tctatgccgc cgatgcaacg ggggcggttg gcaaggccaa ggagttcgcg
      601 gccgcgtagt tcgcacgatc gagcatggac agaacagggg cgggccgggg tcaaatgcct
      661 cggcccgccc ctatttatgc gtcgggggta tcgtcccccg gcgatacgcc ggttatcgat
      721 ggaagaagtg gtcgacgttg tccttggtga tcaacgtgac gcctgtatcg gtgagtccgg
      781 ggacttgcgg tgcgcccgct gcccgccaat cggggaaggc cggaatggtg ttgtggacga
      841 gccagtagag gtagtgtgtg gccatccact cctccatgac gctgttctgg gccatggagt
      901 aggtgagcgt gccgtccttg atgtagggga gcatgtcgtc atcccgatcc atcgcgccgc
      961 ccatgacctt ccctgccagt cccgtctcct tcagcgctcg gcctatggcg gcgcctgccg
     1021 tcgcatcgat gccgatggcc atggtcaggt cggggttagc ctggagcaac tgcgtggaga
     1081 cggtcagcgc gacgttcggg tccgacttct cgtcgaccga cccgacgatc tggatgtctg
     1141 ggtacttggc gaagacttcc ttgtagccgg agactcgctg gccgtgagcg gggttggtgg
     1201 tggtcacgaa gcccaccttg ccgcggccgt tcatgaactt ggccgctgcc tgcccgccga
     1261 tccgtccgac attcacgttg tccgggccca cgaagccgta gcgggcggtg gggtcgttga
     1321 cgtcgtggtt caccactacc accggaatcc cggcggccat ggcttgcttg agcggatcgt
     1381 tcagagactg gtcgtcggca gggaagatca tgatccccgc cggcttggag gcgatcaact
     1441 gctccaagat gttcatctgg gcgactgggt cgtactcgac cggaccgagg taggtggtct
     1501 tcacgccgag ggcgttgccg gcggcgtgga acccctgctg tccgtcaagc cagaacgcga
     1561 tggcggtcac ggcgccgatc cagacgtatt cctcctgggt ctggagcgcc cgccttggct
     1621 ccggcgctgc caacacattg ccgcgcgtca tggccaaccc cgccgcactc gccatggatg
     1681 ccgccagaaa ctgcctcctc gagaggctct tggccattct atctagttcg gcagacatga
     1741 ggccccctcc tgtaatcgcg aatcaatggg ccttgtcggg cccactatgc ccaacagctg
     1801 accaggggtc cggaggaatg cttggagtac tctgccacct cctttcggcg agatcgtcaa
     1861 cccatctcca gtgccacggt gcgtagctaa gtcgtcctga gcctccgcct ggagatcatg
     1921 tcgaggctta ccgcgacgac cagtatggtt cccgtgatca ccccctccca gtatatggag
     1981 acgccgaaca gtgtcatggc gttggacatc atggccatga agaccagacc gagcactgct
     2041 cccaggacgg tgccctctcc ccctgccatg ctgcagccgc cgaggacgca ggcagcgatt
     2101 atccgtagct cgactccagt gaaggccgtt gggacgccgc tggttaggcg ggccgtggtg
     2161 atgatccctg ccagggcggc gaggatccct gaggctatga aggtcaggaa caccacccgg
     2221 tcgacgttga tgccggagag ccgagccgcc ttctcgcttc cacccaagta gtagatctgc
     2281 cgcagcgccc gcgcgcgacg cagcaggatg tccgcggaca ggacgaccac gagcatgtat
     2341 agcactatga gcggcaggct aaagaggtct ccctggccga tgatgttgaa ggactgcggc
     2401 aggttgatca ccgggcgtcc ctcggtatac ccaagcgcga tcccccgagc gacggacatc
     2461 atgcccagcg tggcgatgag cgcattcacc ttggctcgtg tcacgacgaa gccgtttatg
     2521 ccgcccgtga tagcacccac tgccaaccct cccaggatgg ccaggggtat gggaaggtgc
     2581 cctttggcga tcagcgaggc gcacacaatc ccggccagcg ccatgacgga gcccaccgac
     2641 aggtcgaaga agcccccgat tagcagcatg gacatgccca cgacgacgag ggcctccatc
     2701 gagaacgccc gagcaatggc gatcaggttc ggtccgttca ggaatcgcgt ggtggtgagc
     2761 gacagaatga tgccggtggc cagtatcagg gcgagcatgg ccatttcccg cgacacgata
     2821 agccgtgtca tcgactggct tagtccatgg cccgacggtc ctgcgccggt ctgaccatgt
     2881 ggaggggcga ccgtagcgtt gggcgattca cgtgctaccg acgtttcacc ggacatggtc
     2941 atcaccccct gccccgttta ccatcacctc ggtcgcgccc aacgcatggg acgcgatgat
     3001 ctcctccgtt gccccgtctc gcgggaatat gccggctagc cggccttccc gcatgaccgc
     3061 a
//
LOCUS       DATALS010000116         2818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_368850, whole genome shotgun sequence.
ACCESSION   DATALS010000116 DATALS010000000
VERSION     DATALS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368850"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VHS06_02470"
     CDS             <1..885
                     /locus_tag="VHS06_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX2987016.1"
                     /translation="LRAGLMDPKASGRVVSEGPASLVYDGENGMGMVVGTKAMHLCLD
                     KAEKSGVAFTTVRNSNHFGTAGYYAAMALQRDMIGFSITNSPSVMAPWGGKKGFLGTN
                     PLAIAVPAGEERPFILDMATSVIARGAVILAAKRGDTIPPTWAMNADGEPTTDAKEAM
                     NGAVLPLGGHKGFGLSMAIDIMAGILGGGPFGPHIGELYGNSTTQQKVGHFFGAFKIS
                     QFRSVKEFKADMDAMIREVKAQPLGKGFQEIMVAGDPEARVEANRKANGIPLSDAVVG
                     DLTKLGASLELSFDKLVG"
     gene            1133..2314
                     /locus_tag="VHS06_02475"
     CDS             1133..2314
                     /locus_tag="VHS06_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987017.1"
                     /translation="MRNPYRPIAVALLILAVALIPVGCSRGGASLLSELRPSASVVAP
                     TGKGDTPPITVGYTLSRPATIGAYLVGGNGEKLYLQKDEVRPVAGDYSLSFIGAYAPD
                     PSGLERKVVPAGSYPLVIEAVDGAGARQEARTQLQVRDSDTVPPTIAQLTLYPDSISP
                     NFDGIDDTVKITYRSAKVSTVSVYLVSADGKRFVLEREEGQTPGEHSTTWDGQISNQL
                     LASGSYRFIVEAEDAAGNVSAAEKTLKIDSASQPDAHILSVTFQPQKILLGQTVKVEM
                     RVKNTGNTVLKTQGPDPGYTYSSQEGFSTVAGGKYRDQKGLWRVGVDWAGGPGAAGSK
                     YPYRWGFGKDLQPGEEVTVVGYIKMEHTYPQIWVYAGLVQEQVRYWDSEVGRTVIETG
                     Y"
     gene            2314..>2818
                     /locus_tag="VHS06_02480"
     CDS             2314..>2818
                     /locus_tag="VHS06_02480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2987018.1"
                     /translation="MDPVLISIVIVSYNTQDLLRRCLESLVRNPLHSVVSPADGLAMP
                     CMETASDNFSANKASGPPVTCEIIVVDNASPDCSADMVASEFPQVRLIRAGANLGFAR
                     ATNVGLEASRGKLLLLLNPDTEVLGRAMPAMAVYLDGHPKVGVVSPALVYPDGRPQHA
                     AFHFPTLW"
BASE COUNT          541 a          871 c          874 g          532 t
ORIGIN      
        1 cttcgggccg gactgatgga tcccaaggcg tcaggcaggg tagtatctga gggtcccgcg
       61 tcgctggtat acgacggcga aaatggcatg ggcatggtag tcggcaccaa ggccatgcac
      121 ctctgcctgg acaaggcgga gaagtcgggc gtcgccttca ccacggttcg caattccaac
      181 cacttcggga cagccgggta ctacgcggcg atggcgttgc agcgcgacat gatcggcttt
      241 tccatcacca actctccgtc ggtgatggcg ccctggggcg gcaagaaggg tttcctggga
      301 accaacccat tggcgatcgc ggtgccggcc ggtgaggagc gcccattcat cctcgacatg
      361 gccaccagcg ttatcgccag aggtgccgtt atcctcgccg cgaagcgggg cgacaccatc
      421 ccgccgacct gggcgatgaa cgcggatggt gagcccacca ccgacgccaa ggaggcgatg
      481 aacggagccg ttctgcctct cggcggacac aagggtttcg gcctctccat ggcgatcgac
      541 atcatggccg gcattctggg tggcggccca ttcggtcccc acatcggtga gttgtacggc
      601 aactccacca cgcagcagaa ggttggacac ttcttcggcg ccttcaagat ctcgcagttc
      661 cgctccgtca aggagttcaa ggcggatatg gacgccatga tccgagaggt caaggcccag
      721 cctctgggca aaggcttcca ggagatcatg gtggcggggg atcccgaggc gagggtcgag
      781 gcgaaccgca aggccaacgg cattccgctg agcgatgccg tggtcggcga tctgaccaag
      841 ttgggcgcat ccctggaact cagcttcgat aagctggtcg gatagtagca gccactgttg
      901 ccctgtcatc ttgagcgtaa gtgaagtatc tcctcgtgag cggagagatc cttcacttcg
      961 ttcaggatga cacctggcgg atcgtggtct tcgtgggggc gacgcgtgcg tcgcccctac
     1021 tgtatgggag tacacttcga tccttccttg accgccctat ccccctctga taccatgccg
     1081 ctgtcctcgg actgtccatc gggtcggtcc ccaaacgtgg aggagttccc tgttgcgtaa
     1141 tccctaccgg cctatcgccg ttgctctgct cattctggcc gtggcgctga tccctgtcgg
     1201 ctgctccagg ggtggggcgt cgctgctctc agagttgcgc ccctcggcaa gcgtcgtggc
     1261 gccaactggc aagggagaca cgcctccgat taccgtcggt tacaccctct ccaggccggc
     1321 aaccatcggt gcctacctcg tcggcggcaa cggtgagaag ctctatctac agaaggacga
     1381 ggttcgcccc gttgcgggcg actattcact ctccttcatc ggcgcgtacg cacctgatcc
     1441 gtccggactc gagcgaaagg tggttccggc agggagctat cctctggtga tcgaagcggt
     1501 agacggtgca ggcgcccgac aggaggcgag gacccagctt caggtgaggg actccgacac
     1561 ggtgccgccg actattgcgc agttgaccct ctacccggat agcatatctc ccaacttcga
     1621 cgggatcgac gacaccgtta agataaccta tcgcagcgcc aaggtgagca cggtatccgt
     1681 gtacttggta tctgccgacg ggaagcgatt cgtcctagag cgagaagagg ggcagacccc
     1741 cggcgagcac tccaccacct gggatgggca gatcagcaac cagctactag cctccggcag
     1801 ctatcgcttc attgtggagg cggaggatgc ggcaggcaac gtctcggctg cggagaagac
     1861 tctcaagata gactcggcga gccagcccga cgctcacatt ctctcggtca ccttccagcc
     1921 gcagaagatc ctccttggtc aaacggtcaa ggtggagatg cgcgtcaaga ataccggcaa
     1981 cacagtcctg aagacccagg gccccgaccc cggttatacc tacagcagcc aggagggctt
     2041 cagtaccgtc gcgggtggga agtaccgcga ccagaagggg ttgtggaggg tcggcgtcga
     2101 ctgggcgggc ggccccggcg cggcgggctc caagtatccc tatcgatggg ggttcggaaa
     2161 ggacctccag ccgggtgaag aggtcaccgt ggtcgggtac atcaagatgg agcacaccta
     2221 cccgcagatt tgggtctacg ccggtctggt gcaggagcag gttcgatact gggacagcga
     2281 agtggggcgc accgtcatcg agaccggcta ctgatggatc ccgttctgat ctccatagtc
     2341 atcgtctcct acaatacaca ggaccttcta cgtcgctgtc tggaatcgct ggtgcggaat
     2401 cctcttcaca gtgtagtatc gccggccgac ggactggcca tgccttgcat ggaaacagcc
     2461 tccgacaact tctccgcaaa caaggcctcg ggcccgcctg tcacatgcga gatcatcgtc
     2521 gtggacaacg catcgcccga ctgcagcgcg gatatggtcg cttccgagtt tcctcaagtg
     2581 aggctgatcc gtgctggggc taacctcggt tttgcccggg cgactaacgt cggactcgag
     2641 gcaagccgag gtaaactcct cctccttctg aaccccgaca ccgaggtgtt ggggagggcc
     2701 atgcccgcca tggccgtgta cctcgacggc catccgaaag taggcgtcgt gagccccgcg
     2761 ctggtctacc cagacgggcg cccgcaacat gcggccttcc actttccaac actgtgga
//
LOCUS       DATALS010000117         3787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_275625, whole genome shotgun sequence.
ACCESSION   DATALS010000117 DATALS010000000
VERSION     DATALS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275625"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VHS06_02485"
     CDS             <1..490
                     /locus_tag="VHS06_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2987019.1"
                     /translation="RQIKQHFPKVKVLALSAYDSDDYVLEMLKVGASGYMLKDADQSV
                     IVSGIAAVHEGKGRIHPRLAARVFEAFAELSGNGAQHDPAPYDGLTNREIEVLRWLAT
                     GATNRQIATHLWISERTVDNHVQNIYRKLNVSDRVQAILYAVRKGLVSPDGSSTVNAD
                     AS"
     gene            480..2018
                     /locus_tag="VHS06_02490"
     CDS             480..2018
                     /locus_tag="VHS06_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987020.1"
                     /translation="MRRDVGDASVGEALAPPNPRTRRQTLWQRFLCLDQRRLLALLIF
                     LALFGYNFARPTDIDFWWHLKTGELIATTGAIPAADPFSYTVPGQPWVAHEWLWELAI
                     YQIYTWGGYALAAILSAVAVTLTYLILYRLLRRLGSNEFVAAALVLWAAMLAFPGVGV
                     RPRELTQLLLAFFLSRLFLYREGHVRSLWSLPPLMVLWVNVHGQFILGLGLLGLFAIE
                     EVANRLFAGGRSPRHMLVVCLATLAACVNPSGLAMLRYPIDYYLRGENPSFAMVTEFQ
                     SPDFHQPLYLAFAVSLLLLAVLPRSLNRRTVLEALLVAVFTLQALVSVRQIAAYTMVV
                     APLIALRLTDRFKIVRELPPSSVPRPLLALNWLIMLAVVAMGTAYASQPTMSERLQLG
                     REPLIGSLPIAGARFIEDKGLPDPVFHEQTWGGYLIYQWYPQRRVFMDGRVDMYGAAV
                     SREYMSVVSVRPDWNAILDKYGVRTILIQKDSPLSTLLLTDGEWERVFEGNVEAVFVR
                     EGDH"
     gene            complement(2048..2260)
                     /locus_tag="VHS06_02495"
     CDS             complement(2048..2260)
                     /locus_tag="VHS06_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987021.1"
                     /translation="MQEEVLKAGALFGFADKAADFGAALVGESVEGDGVFPDRPHSPD
                     VTPEGTVAYSKEGNHVVFFPFSAPTQ"
     gene            2309..3433
                     /locus_tag="VHS06_02500"
     CDS             2309..3433
                     /locus_tag="VHS06_02500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2987022.1"
                     /translation="MSFSMQRTVQYAASRLWGPLPLLLWTAPAAIMALANITSPMAPL
                     DLSAFLTSGRALLTGEIPYKVDAPNLNAPGSLPFFHALAALDAPWAPVGWYFTSLLAY
                     LASLMIIARLYPKAATPERLAWAMAQGGLWITLTSGQVYALLLLPLTVAYWTASKNRL
                     VVAGLTAGLVIAFKPNFVVWPLLLLISGHRRLGLAMLTGTVLWCIPAALLYGPQVYIQ
                     WLEIVRFGLLPELVHPNNSSLQSLAYRMGLPSAGLPLSAIVLTGVVIWTRRRGSGPSD
                     LAMIALLAAIITSPTAWIGYMCFLMPAFFSRPWNRPIAVAAMLLLIPLWPVIFFGNGW
                     HALWVASYTLAYGSLLVGLLLPIQRSTEVLRTTSLGRTAM"
BASE COUNT          635 a         1286 c         1083 g          783 t
ORIGIN      
        1 tcggcagatc aagcagcact tccccaaggt caaggtcctc gcgctgagcg cctacgacag
       61 cgacgactac gttctcgaga tgctcaaggt cggcgcctcc ggctacatgc tgaaggatgc
      121 cgatcagagc gtgatcgtga gcgggatcgc cgcggttcac gagggcaagg ggcggatcca
      181 tccacggctg gcggcgcgcg tcttcgaggc attcgccgag ctctccggca acggcgcaca
      241 acacgacccc gccccctatg acggcctcac caaccgggag atcgaggtgc tccgctggct
      301 cgccaccggc gcgaccaaca ggcagatcgc cacacacctc tggatcagcg agcggacggt
      361 ggacaaccac gtccagaaca tctatcgaaa gctcaacgtg agcgaccgcg tccaggccat
      421 cctctacgcc gttcgcaagg gcctcgtctc gcccgacggc agctccacgg tcaacgcgga
      481 tgcgtcgtga cgtgggcgac gcttcggtag gcgaagccct cgctcccccc aatccccgca
      541 ccaggcgcca gacgctgtgg cagcgcttcc tgtgtctcga ccagcgccgc ttgctggccc
      601 ttctgatctt cctcgccctc ttcggctaca actttgcccg ccccacggac atcgacttct
      661 ggtggcacct aaagaccggc gagctgatcg ccaccaccgg ggccatccca gcggcggacc
      721 ccttctcata caccgtcccc ggccagccct gggtcgccca cgagtggctg tgggagctgg
      781 cgatctacca gatttacact tggggcggat acgcgctggc ggccatcctg tcggctgtcg
      841 ccgtcacact cacctacctc atcctgtatc gacttctccg caggctcggg tcgaacgagt
      901 tcgtggccgc agcgctcgtc ctctgggccg ccatgctcgc cttccccggt gtcggcgtcc
      961 gcccacgaga gctcacccag ctgctcctcg ccttcttcct gagccgtctc tttctctacc
     1021 gagaggggca cgtccgcagc ctgtggtcgc tcccgccgct catggtgctc tgggtcaacg
     1081 ttcacggcca gttcatcctg ggcctgggcc tccttgggct gttcgccata gaagaggtgg
     1141 caaacaggct attcgccggg gggagatccc cgcgtcacat gctggtcgtg tgcctggcca
     1201 cgctggccgc ctgcgtcaat ccctcaggtc tcgccatgct ccgctacccg atcgattact
     1261 atctgcgggg ggagaacccc tccttcgcga tggtcaccga gttccagtcg ccggatttcc
     1321 atcagccgct atacctcgcc tttgccgtga gcctgctgct gctggcagtg ctcccccgat
     1381 cactcaacag acgaaccgtc ctggaagcac tgctcgtggc cgtcttcacg ctccaggcgc
     1441 tggtctcggt gcgccagata gcagcctaca ccatggtggt cgcaccgctt atcgcgctgc
     1501 ggctcaccga tcgattcaag attgtccgcg agctaccgcc gtcatcggtt ccccgaccgc
     1561 tgctcgccct caactggctg atcatgctgg ccgtcgtcgc catggggacc gcctacgcct
     1621 cccagcctac catgtcggag cggctccagc tcgggaggga accgcttatc ggctcccttc
     1681 cgatagcagg ggcccggttc atcgaggata aagggttgcc cgaccccgtc ttccacgagc
     1741 agacgtgggg cggctacctc atctaccagt ggtacccgca gcgccgggtg ttcatggatg
     1801 ggcgtgtcga catgtatggc gcggcagttt cccgagagta catgagcgtc gtgtccgtgc
     1861 gtccggattg gaacgccatc ctggataagt atggcgtgcg cacgatcctg atccagaagg
     1921 attcgcccct ctcgacgctg ttgctgaccg acggcgaatg ggaacgagtg tttgagggga
     1981 atgtggaagc ggtgttcgtc agagaagggg accactgaca gtccgaccat agggatcggc
     2041 ggggccgcta ctgggtgggt gccgagaaag ggaagaagac gacatggttg ccctccttgc
     2101 tgtaggccac tgtcccctca ggggtcacat cggggctgtg ggggcgatca gggaataccc
     2161 catcaccctc aaccgactct cccaccaggg ccgcgccaaa atctgccgcc ttgtcagcga
     2221 agccaaacag cgcacctgcc ttcagtacct cctcctgcac ccatagcgct cgttcagctg
     2281 tatgatctac aacgtggagg tgaccgccat gtcattcagt atgcaacgaa ctgttcaata
     2341 tgcagcatcg cggctctggg gcccgcttcc gctattgtta tggaccgccc ctgccgccat
     2401 catggctcta gccaacatca catccccgat ggcgccactg gacctcagcg ccttccttac
     2461 cagcggtagg gcactcctta caggcgagat accctacaag gtggacgccc ctaacctcaa
     2521 cgccccaggc tccttgcctt ttttccatgc cctggcggct cttgatgctc catgggcgcc
     2581 ggtgggatgg tattttacat ccctgctggc ctacctagcc tcactaatga tcatcgcccg
     2641 actgtatccc aaggcggcta cccccgaacg cctagcctgg gcgatggccc agggcggact
     2701 ttggataacg ttgacgagcg gccaagtcta tgcactgctg ctactgccgc tcacggtggc
     2761 atactggacg gccagcaaga accggctggt tgtcgcgggg cttaccgctg gactggtaat
     2821 cgccttcaag cccaacttcg tcgtttggcc tctcctgcta ttgatatcgg gccatcgccg
     2881 cctgggcctc gccatgctga cgggaacggt cctctggtgc ataccggccg ccctgctata
     2941 tgggccccag gtatatatcc aatggctgga aatcgtgcgc ttcgggctgc tgccggagct
     3001 ggtccacccc aacaattcct cgctccagtc gctcgcctac cggatgggac taccctctgc
     3061 ggggctgccg ctttccgcca ttgtcctgac aggagtggta atttggacgc ggaggagggg
     3121 atcgggtcca agtgacctcg cgatgatcgc ccttctcgca gcgatcatca catcgcccac
     3181 cgcgtggatt ggctatatgt gtttccttat gcccgccttt ttctcccgcc cctggaaccg
     3241 ccccatcgcg gtcgccgcca tgctattgct gatacccttg tggcccgtca ttttcttcgg
     3301 gaatggttgg cacgcgttgt gggtcgcatc ctatacccta gcttatggtt ctctgctggt
     3361 cgggctgctt ttgcctattc agcgatcaac ggaagtcttg agaactacat cacttggcag
     3421 aacagcgatg taactgctga tgcccgcgtc tctgatacat atccttctac catcagtccg
     3481 cagaaggctg tccgccgctc gctggcggtt gccgccacca gcagctctga ttgacgattc
     3541 accgctcaat tggcggtatt ggcgcacctt tggccctgct tcaaggccgt tgaccttcat
     3601 cgtggtccag acagccatga cggccgggtc gacgtactgc acatccccct tgaggggctg
     3661 ataggacgtg cccaatgtgt cgttcctcaa gccgattgcg tagtgcggag tgctctgcct
     3721 cgccgcccgg tataccggcg acgaggactc ggcggtgcac gggcgtgacg gcacgctcgc
     3781 taccgtc
//
LOCUS       DATALS010000118         6661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_145212, whole genome shotgun sequence.
ACCESSION   DATALS010000118 DATALS010000000
VERSION     DATALS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145212"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            536..1615
                     /locus_tag="VHS06_02505"
     CDS             536..1615
                     /locus_tag="VHS06_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX2987023.1"
                     /translation="MGGQTVVPFGPQHPVLPEPIQVKLTLQDETIVSAVPVFGYCHRG
                     IEKAAELNDLSQNVFLLERVCGICSFMHAMTYCQGIEELMGVEIPERAKYLRVIWSEI
                     QRLHSHLLWIGCFTDAMGFESLFMQSWRYREMILDLQERTAGHRVILSTCTLGGVRRD
                     IDEEKLEVILNTLKELKPGLDQLARVIEKDYTLRKRCVGIGTLSRDQAVSLGVVGPMM
                     RASGIAQDARMLRYAAYGNLKFEPVVETAGDSHARTMVRIRETFQSIDLIREAIDKMP
                     QGEIAIKLRGNPTGEVISRVEQPRGEVMYFIKGNGTKNLERLRVRTPTYANAASLVVM
                     LPGMTMADVPILLHTIDPCFSCTER"
     gene            complement(1671..2066)
                     /locus_tag="VHS06_02510"
     CDS             complement(1671..2066)
                     /locus_tag="VHS06_02510"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX2987024.1"
                     /translation="MTKLVLFVCVHNSGRSQMAEAFFNRLTDGRAISGSAGTEPTDGL
                     NPTVVEAMGEIGIDLTGARPKLLTQHMLDRADRVITMGCSVEESCPALFLPQVEDWGL
                     EDPAGKPMEKVRQIRDEIAVRVKGLLAEL"
     gene            complement(2179..2412)
                     /locus_tag="VHS06_02515"
     CDS             complement(2179..2412)
                     /locus_tag="VHS06_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2987025.1"
                     /translation="MEVKVLGKGCANCMKLEAMVHEVVKEKGLEVNLEHVRDEREIVT
                     YGVMKTPGLVVDGKVVSYGRIPSKEEIASWLSA"
     gene            complement(2506..2865)
                     /locus_tag="VHS06_02520"
     CDS             complement(2506..2865)
                     /locus_tag="VHS06_02520"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2987026.1"
                     /translation="MELGEEAQTELLKAVSHPVRLRILAELVREEECVCHLSSLLKKP
                     QPYVSQQLAALKAAGLVLDRRDAQRIYYRTASPRIGDLLRAIAALAGLPESHGGARHH
                     VPGCPCPKCSPDTLERY"
     gene            3110..3397
                     /locus_tag="VHS06_02525"
     CDS             3110..3397
                     /locus_tag="VHS06_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX2987027.1"
                     /translation="MLLGYELSFGVVVGDPEMMYGYGFGWWGILMMLFWVLIIGGGIL
                     LFVWLFRQVQPAGGGAGPVPRQPIEILRERYARGEITREQFEQMRHDLEEG"
     gene            3408..4109
                     /locus_tag="VHS06_02530"
     CDS             3408..4109
                     /locus_tag="VHS06_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987028.1"
                     /translation="MRAKGLLVAALAALAVGITGLLVLAPITAAPVGPSGWGPSGMMG
                     DGSGMMGGAGMAGMMGGPVDPNATPIGMDRAAAAAQGYIAGFGNPNLQTGEVMEFAAN
                     YYVQVVEKDTGLHALELLVDKYSGAVFPEMGPNMVWNTKYGMMRGMMGGRPFFGQGTS
                     PDLSVSPAQAQELARQYLMNQGLQLDLEEPDRFYGYYTIHTVRDGAIEGMLSVNGYTG
                     QIWYHTWHGPFVQTR"
     gene            complement(4239..4649)
                     /locus_tag="VHS06_02535"
     CDS             complement(4239..4649)
                     /locus_tag="VHS06_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HEX2987029.1"
                     /translation="MQGLQARYDLNQQELWPLVVGLGSGMSRRGFVCGAVTGGAIACG
                     LVTAHQLDSTRESRKQLRETTYSRVQEMTRRFEKKFGTVDCSAMTGCDFLTPEGQATF
                     REKKLMDSVCRPAVQLIVETLVDLIEGDGAVPSQ"
     gene            complement(4942..5514)
                     /locus_tag="VHS06_02540"
     CDS             complement(4942..5514)
                     /locus_tag="VHS06_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX2987030.1"
                     /translation="MELLEAIKGRRAVRSYADTPVARETIEELIGAAVLAPSAMNSQP
                     WAFGVVTGREMLLGYSDRAKAALLAIMDQLPALERYRARLSNPDFNIFHGAPALVVIY
                     AKPGAGVAPETDCALAAQNLMLAARGQGIGSCWIGFARVLFDSPELKRELGVPAEYRV
                     VAPIILGYPKGDMPAPTPRNAPEMLFWVQG"
     regulatory      5780..5957
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6084..>6661
                     /locus_tag="VHS06_02545"
     CDS             6084..>6661
                     /locus_tag="VHS06_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2987031.1"
                     /translation="MCPTPHSIETELAQVGCSHHDPTGAISVPIYQTSLFRHPGLGQS
                     TGYDYARTANPTRTALEEAIAQLEGGARGLAFASGMAAITTIAMLFRAGNHLLLSDDL
                     YGGTYRLFTKTLAPFGLTADCVDTANLDEVEEAITPATRALLIETPTNPMMKISDLAA
                     LANLARRHRILTIVDNTFMSPYLQRPLALGCDL"
BASE COUNT         1323 a         2167 c         1953 g         1218 t
ORIGIN      
        1 ggagggtcgg cacgccggat cagcaaccct tcaggggtct cctggaccat gagatccgca
       61 ccggggtcca gtccccagcg acgtcgatat acatcgggca actggatatc gccgttttca
      121 ccgatccgcg ctgttgatac catcgtccgt agcccctcag cacttctttt ccggacagca
      181 cacggccgac cctcggtttc cggcggcctg tgcataaacg attatctgtc ttcccttggt
      241 atctcccgcc ttcggtatca gctcgtacta tgccgtcgca accccagatc gccattatac
      301 cagcccgaga ttcggcaccg tacgcacgct cggttaaccc cggccactcc taagtacatc
      361 accgaaacga gatcgcaaac tcggcttcat gatctatgat atagtagtga cgtcggcggt
      421 gggcgggggc agaagcatgc tggagtgcgc ccacagcagc gactcgacac ttcgattccc
      481 ctcacgaaat ccatggcggt gaccccggct ggggtctccc cacttgaggt gagcgatggg
      541 tggacagacg gttgtccctt tcgggcccca gcatccggtc ctgccggaac cgatccaggt
      601 gaagctgaca ctccaggacg agacgatcgt cagcgccgtt cccgtgttcg ggtactgcca
      661 ccgaggaatc gagaaggctg cggagcttaa cgacctctcg caaaacgtct tcctgctgga
      721 gcgagtctgc ggcatctgca gcttcatgca cgccatgacc tactgccagg ggatcgaaga
      781 gctgatgggg gtggaaatcc ccgagcgggc caagtacctg cgggtcatct ggagcgagat
      841 ccagaggctg cacagccacc tgctgtggat cggctgcttc accgacgcca tgggcttcga
      901 aagcctcttc atgcagagct ggcggtaccg agagatgatc ctggacctcc aggagaggac
      961 ggcaggccac agggtgatcc tctccacctg tacgctgggg ggggtgcgcc gggacatcga
     1021 cgaagagaag ctggaagtaa tcctcaacac gctgaaggag ctgaaacctg gtctggatca
     1081 actcgccagg gtcatcgaga aagactacac acttcgcaag cggtgcgtgg gcataggaac
     1141 cctatccagg gatcaggcag tgagtctggg tgtcgtcggc ccgatgatgc gcgcaagcgg
     1201 tatcgcccag gacgctcgga tgctcagata cgccgcctac ggcaatctca agttcgagcc
     1261 ggtggtagag actgccggcg acagccacgc ccgaaccatg gtccgcatca gggagacttt
     1321 ccaatccatc gatttgatac gggaggccat cgacaagatg ccccagggcg agatcgcaat
     1381 caagctgagg gggaacccca ccggagaggt gatatcgagg gtggagcagc ctcgaggcga
     1441 ggtgatgtac ttcatcaagg gcaatggtac caagaacctg gagcggctcc gggtcaggac
     1501 tcccacctat gccaatgccg catcgctggt ggtgatgctg cccggcatga ctatggccga
     1561 cgtgccgatc ctgctacaca cgatcgaccc atgcttcagc tgcactgaga ggtaagcgtt
     1621 cttggagcgg gcccgggtgg cgaccttctg gacggcaccc gggccgcacc tcacaactcc
     1681 gccagaagcc ccttaaccct gaccgcaatc tcatccctga tctggcgcac cttctccatc
     1741 ggcttgccgg cggggtcctc cagtccccag tcctccacct gaggcaggaa gagggcaggg
     1801 caggactcct ccaccgagca gcccatggtg atcacgcggt ccgcccgatc cagcatatgc
     1861 tgcgtcagca gcttaggcct agcgccggtc aggtcgatcc cgatctcccc catggcctcc
     1921 acgaccgtgg ggtttaaccc atcggtaggt tcagtaccgg cggagccgga gatggcccga
     1981 ccgtccgtca gccgattgaa gaacgcctcg gccatctggc tgcggccgga gttgtgtacg
     2041 cagacgaaga gcaccagctt ggtcacgtgg taagcctttc aaacagtcta tccacaggaa
     2101 tccaagtgta gcttcacata gtttcgcacg gcaagcacat ccgtccctcc tatgcctcgt
     2161 ccacgccggc ccgaaaccct atgcgctaag ccagctagcg atctcttcct tggaggggat
     2221 gcggccgtag gagaccacct tgccgtccac caccagcccc ggggtcttca tgacgccgta
     2281 ggtgacgatc tccctctcgt cccgaacgtg ctccaggttg acctccagtc ccttctcctt
     2341 caccacttcg tggaccatcg cctccaactt catgcagtta gcgcaaccct tgccgagaac
     2401 cttgacctcc atcgatctcc tcctagacga ttagattgaa cagatacccc gtgacgacta
     2461 tggcgacgcc caccacgccg acgaaggcgg cgatgaactg cggcttcagt atctttcgag
     2521 ggtatctggg ctgcacttgg ggcagggaca gccaggaaca tgatgccgcg ccccgccgtg
     2581 agactcaggc aaaccggcca gggccgcgat cgcccgcaac agatccccta tacgcgggct
     2641 ggccgtccga tagtagatcc gctgggcatc cctacgatcc aggaccaacc ccgccgcttt
     2701 cagggcagcg agctgctgtg agacgtaggg ttgcggcttc ttcaagaggg aagacagatg
     2761 gcagacacac tcctcctccc ggaccagttc cgccagaatg cgcagccgga ctggatggga
     2821 taccgccttc agtagctcgg tctgcgcctc ctcaccgagc tccaccttct ccaaaccgcg
     2881 ccctcctcca tgccattctt tgcatatatg gtattgtgca tgttggatga agtcaagtgg
     2941 cgcgttgcca gaatcgggcc agacaggcga caccggatgg ctgttcgggt cgctcagaca
     3001 cggtacagaa tttgacaggg atgctccttg cgaaagcgca aatcacgagt ctgcaactgg
     3061 tgaccgtctt gcagacgaga tcggaccagc cgccgaaccg atagaggcag tgcttcttgg
     3121 ctacgaactc tcgttcggcg tcgtggtggg agatcccgag atgatgtacg gatacggctt
     3181 cggctggtgg ggaatcctga tgatgctctt ctgggtgctg atcatcggcg ggggcatctt
     3241 gctcttcgtg tggctcttcc ggcaggtgca acccgcggga ggcggagcag gtcccgtccc
     3301 gagacagccc atcgaaatcc tgagagagcg atacgctcgg ggcgagatca ccagagagca
     3361 attcgagcag atgcgccacg atctcgagga agggtagggc tcgaccggtg cgagccaaag
     3421 ggctgctcgt tgcggcactt gctgccctcg ccgttgggat caccggactg ctcgtcctag
     3481 cgccgatcac agccgcacca gtcggtccca gcggctgggg acccagcggc atgatgggag
     3541 acggtagcgg catgatgggt ggtgccggca tggccggaat gatgggcggc ccggtggacc
     3601 ccaacgctac ccccatcggc atggatcgag ctgccgccgc tgcccaaggc tacatcgccg
     3661 gcttcggcaa cccgaacctg cagacaggtg aagtgatgga attcgccgcc aactactacg
     3721 tccaggtggt tgagaaggac actggactcc acgcactcga actgctggtc gacaagtaca
     3781 gcggggccgt gtttcccgag atgggaccca acatggtgtg gaacaccaag tacgggatga
     3841 tgagaggcat gatgggcggc cgtcccttct tcgggcaagg aacgtcgccg gatctctccg
     3901 tcagccccgc gcaggcccag gaactggcca ggcagtacct gatgaaccag ggattgcagc
     3961 tggacctgga ggaaccggat cgcttctatg gctactacac catccacacc gtgcgagatg
     4021 gtgccataga aggaatgctc tcggtgaacg gctacaccgg ccagatctgg taccacacct
     4081 ggcatggtcc cttcgttcaa acccgttgac ggccagtggc ggacgcggac atgtcgctgc
     4141 atctggtgtg ttttaccctg gcacaacccc accgtcccat gatccggcaa tcggcgttga
     4201 cggctgctta gctcggcgga ccgagttcct tgggagcgtc attggctcgg gacagccccg
     4261 tccccctcta tcaaatcgac cagcgtctcc actatcaact gaactgccgg ccggcagacg
     4321 ctgtccatca gctttttctc cctgaaggtt gcctggccct cgggggtcag aaagtcacac
     4381 cccgtcatgg cactgcagtc gacggtgcca aacttcttct cgaaacggcg cgtcatctcc
     4441 tgaactcggc tgtaggtcgt ctcacgcaac tgctttcggg actcgcgggt ggagtcgagt
     4501 tgatgcgcgg taaccaggcc acacgcgatg gcgcctccgg tgacggcgcc gcagacaaac
     4561 cctcgccggc tcatgccgga tcccaggccg accaccaggg gccacaactc ctgctgattc
     4621 agatcatatc gggcttgcag accctgcaag acggattggg ctcaattgta cccagaggtg
     4681 aagtattcgt cggccagggc caacgtctcc gacagagcgg gtttttcagt caacaactcc
     4741 aagcctccac acctagattc ttggctacgt tgtcgcagca ccggccattg tgatgccggg
     4801 cgcggtcgac gtacccggtc cacctccaca gaagaggtga cacatagtgt catgctgaac
     4861 gcagcgaagc atctcctcgt ggccggagag atcctttact ccgttcagga tggcacctgc
     4921 caggagcgct tcggcaaagg tctagccctg gacccagaaa agcatctccg gggcgtttcg
     4981 gggtgtcggc gccggcatgt cacccttggg gtagcccagg atgatcgggg cgacaacccg
     5041 gtactccgcc gggaccccca gctcgcgctt gagctccggg ctgtcgaaca gcacgcgggc
     5101 gaacccgatc caacagcttc cgatcccctg gcctcgcgca gcgagcatca ggttctgcgc
     5161 cgccaacgca caatcggtct ccggggcaac tcccgccccg ggcttggcgt agatgaccac
     5221 cagggcgggg gctccgtgga agatgttgaa gtcaggattg ctcagcctgg ctcgataccg
     5281 ttccaatgcc ggcaactgat ccatgatcgc caacagggcg gccttggctc ggtcggagta
     5341 gccgagcagc atctccctgc cggtcaccac gccgaaggcc cagggctgcg agttcatggc
     5401 gctgggggcc agcacggccg ccccgatcaa ctcctcgatg gtttcccgcg ccaccggggt
     5461 atcggcgtaa cttcgcaccg cccggcggcc cttgatggct tccagtagtt ccatttcacc
     5521 cctcccaaga ctgagtctcc cccccagcgc ggacaacatc gggaccttcc ctctgccgag
     5581 tgcacacgga catggatcta cattctacat cttcgtgcct gcgacgacga atacttgaac
     5641 tcgacgagaa gcgccgccgt cggttgacat acaacctgcc tcctgatata aattcgacta
     5701 ttccgagcgg ccaactcatg aacaatggag tgcggcctca agtactttcg tcctgccgaa
     5761 ctgaatagtc gcctgcaacg ccttatcagg agtggtggag ggagacggcc cttcgaagcc
     5821 cggcaaccag cggctgatga agccgccacg cgggtgatcg atgcctcagc agcccatccg
     5881 gcggttcgcc cgccggctcg ggcagtggca tcgccccgca ggtgccaatt ccggcggcat
     5941 gatccgcaag ataaggggtg cgtagagcgt aggcatccct ctttgcctgt aaaaagaggt
     6001 cctttgatgc ataccctgcc gtgccctttt gagcagggcg ggcggcatca aatcgaccta
     6061 tcggcagcaa ggagggtttt cctatgtgtc cgaccccgca cagcatcgag acagaactcg
     6121 cgcaggtggg ctgctcccac cacgacccca ccggggcgat cagcgtcccc atctaccaaa
     6181 cctccctctt ccgacaccct gggctcggcc agagcaccgg ctacgactac gcccgcactg
     6241 ccaacccaac ccgcacggca ctggaggagg ccatcgcaca actggagggg ggagcacggg
     6301 ggctcgcctt cgcctccggc atggccgcca tcaccaccat cgcgatgctg ttcagggccg
     6361 ggaaccatct gctgctctcc gacgacctgt acggcgggac ctaccgcctg ttcaccaaga
     6421 cgctggcccc cttcggactc acggcagact gcgtggacac tgccaatctc gacgaggtcg
     6481 aggaggctat cacgcccgcc acacgcgccc tgctgatcga aacccccacc aacccgatga
     6541 tgaagatcag cgacctggcg gcgctggcca acctggcccg tcgccacagg atcctcacca
     6601 tcgtggacaa caccttcatg tcgccctacc tgcagcgccc gcttgcgctg gggtgcgacc
     6661 t
//
LOCUS       DATALS010000119         4171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_50132,
            whole genome shotgun sequence.
ACCESSION   DATALS010000119 DATALS010000000
VERSION     DATALS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50132"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VHS06_02550"
     CDS             <1..642
                     /locus_tag="VHS06_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX2987032.1"
                     /translation="GFDVIPCGGTHVRSTAEIGMIKMVRWERRSGNVRVEFLCGRRAL
                     LDYRTKNQSVVELAAALSVRDREVPEAVERLQREGDGLRHQIGQLRNRLQDYQAADLS
                     RQAESVGSALVVATKLDEATPDDLKHLALRITEEPGRVALLATEGEKTQLVFARSEDI
                     TVDVGVLLRQVCAPFGGRGGGKPNLAQGGIPEPGRATLVLQGAVEALRSLLAV"
     gene            complement(628..954)
                     /locus_tag="VHS06_02555"
     CDS             complement(628..954)
                     /locus_tag="VHS06_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987033.1"
                     /translation="MAVNPIRRVGSRIAGEAKKRHEGAEGERQGMTKRNRPDGVGALP
                     SRSNTMARVRQFVGGSHQLAITSQSLAELLGLMETECRIALETLVVGGHLRKLIQEPH
                     PPLYCK"
     gene            1081..1605
                     /locus_tag="VHS06_02560"
     CDS             1081..1605
                     /locus_tag="VHS06_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX2987034.1"
                     /translation="MSANRMDPLGMISLRDAIDRLFGVGFSRGLLGRRGMGVGEDQWI
                     PVNMFELPEGVMIVAPMPGLQADDVELSVAGTILTLRGRRTPGQEDREYMVKEWDYGP
                     YSRTIDLPFEVDTDHIIATLDNGVLTVNLRKSEAAMPKRIHIRSGAERSATGGEVHAS
                     PQGIERERESESRH"
     gene            2306..3676
                     /locus_tag="VHS06_02565"
     CDS             2306..3676
                     /locus_tag="VHS06_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX2987035.1"
                     /translation="MISASERTTKILLDESEMPTHYYNVAADLPKPLDPPLHPATGKP
                     LGPEDLAPLFPMELIRQEVSTERYIEIPEEVQDVYRLWRPSPLIRARNLEKALDTPAK
                     IYYKYEGASPAGSHKLNTSVAQAYYNKKEGIKRLSTETGAGQWGSALSMACSFFGLEC
                     KVYMVKVSYFQKPYRRSLIETFGASVVPSPSPDTAAGRAILAKNPDSRGSLGIAISEA
                     VEDAVSREDTHYSLGSVLNHVVLHQTVIGQEAKAQLAKVDEYPDIVIGCVGGGSNFGG
                     IAFPFMQDRLAGRKLQFIAVEPAACPTLTKGVFAYDYGDTAKLAPVAKMYTLGHDFMP
                     EGIHAGGLRYHGMSPLISALYDQGYIDAVALPQLGVFDAAVKFARLEGLVVAPESAHA
                     VKAAMDAALRCKESGEAKTILFNLSGHGNFDMAAYDAYFAGKLQDYDYPEEKVQEALS
                     DLPKVD"
     gene            complement(3733..>4171)
                     /locus_tag="VHS06_02570"
     CDS             complement(3733..>4171)
                     /locus_tag="VHS06_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX2987036.1"
                     /translation="ADAVSTADVVIGSVLIPGTKAPKLVTEEMVGTMKQGSVVIDVAV
                     DQGGCIETSHVTSHTNPTYLVDGVVHYAVPNMPGAVPRTSTYALSNATLPYAAKLATL
                     GFTAAVKKVPELAKGVNSYRGKVTYQGVADAFGYQCVPLNELL"
BASE COUNT          755 a         1224 c         1426 g          766 t
ORIGIN      
        1 ggatttgacg tgatcccctg tggagggacc cacgttcgtt ccacggccga gatcggcatg
       61 atcaagatgg tgcgatggga gcgcaggagc ggcaacgtga gggtggagtt tttgtgcggc
      121 cggcgggcgc tgctggacta ccgtacgaag aatcagtctg tggtggagct ggccgcggcg
      181 ctcagcgttc gggataggga ggtgccggag gccgtcgagc ggttgcagcg ggagggcgac
      241 gggcttcgac accagatcgg ccagcttcgg aatcggctgc aggactatca ggcagcggac
      301 ctgtcacggc aggcggagtc ggtgggcagc gccctggtgg tggcgacgaa gttggacgag
      361 gcgacgcccg acgacctcaa gcacctggca ctccgaatca ctgaggagcc ggggcgggtt
      421 gcactgttgg caacggaggg ggagaagacg cagctggttt tcgcccgttc cgaggacata
      481 acggtcgacg tgggagtcct gcttcgccag gtgtgcgctc cctttggggg taggggaggc
      541 ggtaagccga acctcgcgca gggcggaatt cccgagccgg gtagggcgac attggtgttg
      601 cagggggcgg tggaggcgct ccgctcacta cttgcagtat agcggcggat ggggctcctg
      661 gatgagcttg cgcaggtgac cgccgacaac caatgtctcc agggcgatcc ggcactccgt
      721 ctccatcagc cccagcagtt ctgctagcga ttggctggtg atggccagct ggtgagaacc
      781 tccgacgaac tgtctcaccc tggccatcgt gttggaccta gacggtaacg caccgacgcc
      841 gtcggggcgg tttcgtttgg tcatgccttg cctttcacct tcggcaccct catggcgctt
      901 cttcgcctct ccggcgatcc ggctcccgac acgccggatt gggtttactg ccatcgcttc
      961 atctccctcc aattgccgtg acttgctgtc ttcggcagca atagccgtgc ccctcttcag
     1021 cactggctcg catcctgcta cccggctgag gtgagatatc aggagggagg gaccgttggg
     1081 atgagcgcaa atcgaatgga ccccctgggc atgatctccc tcagggatgc catagaccgg
     1141 ctcttcggcg tcgggttctc taggggcctc ttgggccgca ggggcatggg tgtcggcgag
     1201 gaccagtgga tacctgtgaa tatgttcgag cttccagagg gagtgatgat cgtcgcgccg
     1261 atgcccgggt tgcaggcgga cgacgttgag ttgtcggtcg cggggactat actgaccctc
     1321 aggggacgcc ggactcccgg gcaggaggac cgcgagtaca tggtgaagga gtgggactac
     1381 ggaccttaca gccgtacgat cgacctgccc ttcgaggtgg acaccgacca catcatcgcc
     1441 acgctggaca atggggtgct cacggtgaac ctccgcaagt ccgaggcggc tatgccgaag
     1501 cgtatccaca ttcgcagcgg ggcggagcga tcggcgacgg gtggagaggt ccacgccagc
     1561 ccgcagggga tcgagcggga gcgggagtcg gagagccgac attaggttcc tggcgcgagg
     1621 agacgatccc ctgatgtcgg ggcgtcgccc ttgctggaaa tggtcgactc ctcgcgccat
     1681 tgcatcgtcc ggttgtgcga tgtatactcg ctgcaattga tcgccgaaag acggtgatcg
     1741 ggactagtaa gcggttgtgt ccagggtcag agagccgggg taggtgagag ccggcaccag
     1801 atgcaccgcc gaatggaccc gtgagttgca ggcccaaagg gtgataccga gtaggccgtg
     1861 ccggattggc ccccgttatc agggcacggg tatcggagtc tccgcggcag ggttgaggcc
     1921 cgcggcggaa ggtttcccgt acctcagtga gatgccacgg ttcccgcgtc ggttgggtcc
     1981 gtggtaacca gggtggtacc gcgaaggcaa agcctctcgt ccctagggac gagaggcttt
     2041 ttgttttgcc ggaggatctg gatggagagg atggaaaaga ctggggtgac gaaggtggca
     2101 gcagccggga tgtccgttgt ggcgctgtcg cccgtgtcac ccttctcgtg gtgatggggg
     2161 cggccggcaa ccgccccact cggatgcagt gtctacagta ggggcgacgc acgcgtcgcc
     2221 cgagccaggc gacatatatg tcgcccctac gtgaaccggc aggcaggttg tctgcgcacc
     2281 cagcactcag attaggagaa gaaagatgat aagcgcatcc gagcgcacca ccaagatcct
     2341 gctcgacgag agcgagatgc cgactcacta ctacaacgtt gccgcggatc tgccgaagcc
     2401 gctggatccc cccctgcacc cagcgaccgg caagccgttg ggccccgagg acctggctcc
     2461 gctattcccc atggaactga tccggcagga ggtctccacc gaacgctaca tcgagatccc
     2521 ggaagaggtg caggacgtct acagactgtg gaggccctcc ccgctgatca gggcgaggaa
     2581 tctggagaag gcgttggata cccccgccaa gatctactat aagtacgagg gggcaagccc
     2641 cgccggaagc cataagctga acacctctgt cgcccaggca tactacaaca agaaggaggg
     2701 catcaagcgg ctctccaccg aaaccggtgc ggggcagtgg ggcagcgctc tttccatggc
     2761 ctgttccttc tttggcctgg agtgcaaggt gtacatggtc aaggtgagct acttccagaa
     2821 gccctaccgc cgctccctga ttgagacgtt cggcgccagc gttgtgccga gccccagccc
     2881 ggacacggcg gcggggaggg cgatcctcgc caagaacccc gattcgcggg gtagccttgg
     2941 gatcgccatc agcgaggctg tggaggacgc ggtgtcccgg gaggacaccc actactcact
     3001 gggtagtgtg ctgaaccacg tcgtgttgca tcagactgtg atcggccagg aggcgaaggc
     3061 tcaactggcc aaggtggacg agtaccccga catcgtgatc ggctgcgtcg gcggcggcag
     3121 caacttcggc gggatcgcct tcccctttat gcaggaccgg ttggcgggcc gaaagctgca
     3181 gttcatcgcg gtggagcccg cggcctgtcc tactctcacc aagggggtct ttgcctacga
     3241 ttacggcgac actgccaagt tggcgccggt ggcaaagatg tacaccctcg gccacgattt
     3301 tatgccggaa ggcatccacg cgggcggcct gcgctatcac ggaatgtcgc cgctaatcag
     3361 cgcgctgtac gaccagggct acatcgacgc cgtcgcgctt ccgcagttgg gtgtattcga
     3421 tgcggcggtg aagttcgccc ggctggaggg gttggtggtg gcgccggagt cggcccatgc
     3481 cgtgaaggct gccatggacg ccgcgctgcg ctgcaaggag agcggtgagg ccaagaccat
     3541 cctgttcaac ctcagtggcc atggcaactt cgacatggct gcctacgacg cctactttgc
     3601 cgggaagcta caggactacg actaccctga ggagaaggta caggaggcgc tctccgacct
     3661 gcccaaggtc gactgagggg gataggggtt aggggatggg ggatggtctt cctatccccc
     3721 atcccccatc ccctacaata gttcgttcag cggcacgcac tgatagccga atgcgtcggc
     3781 gacgccctgg taggtcacct tgcctcgata gctgttcacg cccttggcca gttcggggac
     3841 cttcttgacc gccgccgtga agcccagcgt ggccagcttg gccgcgtagg gcagggtggc
     3901 gttggacagg gcgtaggtgg aggttctggg caccgcgccc ggcatgttgg ggacggcgta
     3961 gtgcaccacc ccatccacca ggtaagtcgg gttggtgtgg ctggtcacat gggaggtctc
     4021 gatgcagcct ccctggtcta ccgccacgtc tatcaccacg ctgccctgct tcatcgtccc
     4081 gaccatctcc tcggtcacca gcttcggggc cttggtgcca gggatcagca cggagccgat
     4141 aaccacatcg gcggtggata ctgcgtcggc g
//
LOCUS       DATALS010000120         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_369106, whole genome shotgun sequence.
ACCESSION   DATALS010000120 DATALS010000000
VERSION     DATALS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369106"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /locus_tag="VHS06_02575"
     CDS             complement(<1..1892)
                     /locus_tag="VHS06_02575"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEX2987037.1"
                     /translation="MDKTTRTRGVGGRPGDAAQSTRPRWTLPLLAALLVLVFLIVLTT
                     GVALGYQVVHSSDVYPGVRVGSVQLGGMPREKAIATLRPMYEERAKQGLSLRMADIEQ
                     TVSMADLGVTLDPSQAVDAAFEIGRKGGLMERFGTQFQALRQGYQVETPGVKIDRTKL
                     DALLAKLVQQVDRPVKDAQLQFGDGSVRVDASVVGRKLDTPGSALAIERAVGNGTSTL
                     DLPVTLTQPKRTEKDLEEARVKLSSLLSGPVTLEFEGRKWSLTPKDIVGLVAVDDKGG
                     AGAPAVSVRDDSLKQYVEKVAGEVDQPKSNARLDWNGGNLKVIKQGQDGRKLNREKAL
                     TALTSAITGSQRTVSLPVEVDAAVGGSSDLTKLGIKERIEYGQTTVAGVPEKVYNIKL
                     ASSRLNGTLVAPGETFSFNKELGPTTLNSGFQIGFGISVNNGEMQTVPSVAGGICQVA
                     TTLLHAVFWAGYPIEERYPHMYWISSYGQPPKGITGLDTTVEDPVLDFKFINSSDSYL
                     LIQSSVVGTTLEFALYGTKPIWKVDVEGPIITNVVKADPATVRQEEPTWNVGRELWVE
                     RATDGMDVDIIRKVTQGSDVRTLHLKSRYQPSRNVLMVGTKGAQAAPAVTQGAGSQSG
                     TPAPAAT"
BASE COUNT          388 a          656 c          553 g          407 t
ORIGIN      
        1 gttgccgcag gcgcaggcgt accggactga ctgcccgctc cctgcgtcac cgctggagca
       61 gcctgcgccc ccttagtacc aaccatcaac acgtttctgg aaggctggta tctgctcttc
      121 agatgaaggg tcctgacgtc actcccctgg gtgactttac ggataatgtc gacgtccatc
      181 ccgtcggtgg cccgctccac ccacaactca cgccccacat tccacgtcgg ctcttcctgg
      241 cgaacggttg ccgggtctgc cttgacaacg ttggtaatga tggggccctc cacatccacc
      301 ttccagatcg gcttggttcc atacagggca aactcgaggg tggtacccac aacactgctc
      361 tggatcaaaa ggtagctgtc cgagctattg atgaacttga agtccagaac agggtcctcc
      421 accgtggtgt ccaacccggt gatccccttg ggcggttgtc cgtagctgga gatccagtac
      481 atgtgaggat agcgctcctc aatcgggtac ccagcccaga acacggcgtg gagcagagtc
      541 gtcgccacct ggcagatgcc gcccgctacc gagggaacgg tctgcatctc gccgttattg
      601 accgagatac caaacccgat ctgaaaaccg gaattcagcg tggtcggccc caattccttg
      661 ttgaaggaga aggtttcccc cggggcaacc agcgtgccgt tgagccgaga tgaggcaagc
      721 ttgatgttgt agaccttctc gggaaccccg gcgacggtcg tctggccgta ctctatcctc
      781 tccttgatcc ccagcttggt gaggtccgac gatccgccta cggccgcgtc cacctctacg
      841 ggcaggctca cggtccgctg agagccggta atggcagacg tcagcgccgt gagcgccttt
      901 tcgcgattca gcttcctgcc gtcctgacct tgcttgatga ccttaaggtt cccgccgttc
      961 cagtccaatc gagcgttgct cttaggctga tcgacttcac ccgcgacctt ctccacatac
     1021 tgcttcaggg agtcatcgcg caccgacacg gcaggagctc cggcaccgcc cttgtcatca
     1081 accgcgacga gacccactat gtcctttggc gtcagcgacc atttgcgccc ctcgaactcc
     1141 agagtaacag gaccagagag caggctgctc agcttcaccc tggcctcttc gaggtccttc
     1201 tcggttcgct tgggctgtgt gagggtcacc ggaagatcga gtgtggacgt accgttgccg
     1261 accgctcgct ctatggcgag agcagaaccc ggagtatcca acttgcggcc aactaccgac
     1321 gcatctaccc gcaccgagcc gtctccgaac tgcagttggg catccttgac cggacggtct
     1381 acctgctgaa ccagcttggc gaggagcgca tcgagcttcg tccgatcgat cttcaccccg
     1441 ggagtctcca cctgatagcc ttgccgcaga gcctggaact gagtcccaaa ccgctccatc
     1501 agaccgccct tgcggccgat ctcgaaagcg gcatcgactg cctggctcgg atcgagcgtc
     1561 acgcccaggt cggccatgct cacagtctgc tcgatatcgg ccatacgcag cgaaagcccc
     1621 tgctttgccc gttcctcgta catcggacgt agcgtggcaa tcgccttctc gcggggcatt
     1681 ccgcccagtt gcaccgagcc aactcgaacg cccggataga catcgctcga gtgtacgacc
     1741 tggtagccca gggcaacacc ggtggtgaga acgattagaa aaacaagaac aagaagtgca
     1801 gccagcagtg gaagtgtcca gcggggccga gttgactgcg cagcatcgcc ggggcggcca
     1861 cccacacccc gcgtccgggt cgtcttatcc atcaggggat caacctctcg ccatgaacct
     1921 tccctttcgc gccattggga atgagggcgg gaaggttggg acttgattgt cctagatgat
     1981 tggcctgctg tcaacccaat ttac
//
LOCUS       DATALS010000121         5883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_191534, whole genome shotgun sequence.
ACCESSION   DATALS010000121 DATALS010000000
VERSION     DATALS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191534"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VHS06_02580"
     CDS             complement(<1..764)
                     /locus_tag="VHS06_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HEX2987038.1"
                     /translation="MDEVRFWGVARTPEQIAASYAIRTRQREIGLLGAWQFDKGSGTT
                     AVDSSAADGRDGTLVNGPLWVLSGAQLGIPTNSAPIATGESYTTAEDTTLSVSAPGVL
                     ANDSDPDGDALDISLQSGPSHGTLALNANGSFNYTPTPNYNGTDSFTYKIGDGSPDFD
                     TATVSITITPVQDPPIAVNDSFPSMLENATLVYNGAFGVLHNDSDADSDPLTVSLVAG
                     PAGGTLSLGSDGSFTYSPGDFNGTANFTYRVCDPSNA"
     gene            complement(757..1995)
                     /locus_tag="VHS06_02585"
     CDS             complement(757..1995)
                     /locus_tag="VHS06_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX2987039.1"
                     /translation="MAPTATSVRLRTIQRSFGIQSITQPSAYTTAVQADGPYGWWRLG
                     QVGVGAPDEGNGGSVPDVISTSSNVALQPGILAGDPDSAASFNGSSSYLRINNSTDLQ
                     LAPGDSFSVEMWVKPANTTQTGTLFAKTKDPDTFSVKILDNAGKAEFCPGSGTGTAPT
                     GIWNCSTSAGSIPADQWTHLAFTYDGATKTTQTYINGQADPVTGNFSLTGVTAGFGFV
                     SLGNYGMANQDYFNGLMDEVSFYRKVLSADRIAAHYWAGISGYALQFDGADDYLNVPL
                     PPGGHQTAYTLEGWVKPSSTAAQNLMAAASAGQETSQFTNLLRINSSGRFEHYLTDNG
                     IPKVVTGSTVVVPNTWYHVAAVAEDGGSMRLYVNGVEQGTPVAVGDVAPPMDYRMVSG
                     GGPRGRQRSVELRLLPGHHG"
     gene            1994..2398
                     /locus_tag="VHS06_02590"
     CDS             1994..2398
                     /locus_tag="VHS06_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987040.1"
                     /translation="MAVAVAVAEVTVAVAVEAAIVGVALGVVAVIVAVVAGAMPAVGL
                     PLGMAPGDTEGTASTVDVEVAVMVGVAVSGPALVVGEGPRISGLSDAAVPTPAEAISK
                     QSSGTTMREKTREPSWLFIWDLLDVTESVKCA"
     gene            complement(2925..3497)
                     /locus_tag="VHS06_02595"
     CDS             complement(2925..3497)
                     /locus_tag="VHS06_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987041.1"
                     /translation="MLTRQAGRYIVTLVAALALPLFAVANCGPAAQSVAKEATPTPVP
                     TITPTPLPAALEGLQRQYARSDDGSSGAKVTVTWLTADYLKQDPAAGQGFDPQKQLIF
                     RVVVDAPGSKISVWDLRGMLYLREDGGKEYGTPTWVPTAGSGTLSGVAAFSRTDARAR
                     PVPRDGARYIEFVIRDLQQVRERVFRWPLP"
     gene            3685..4830
                     /locus_tag="VHS06_02600"
     CDS             3685..4830
                     /locus_tag="VHS06_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019381464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HEX2987042.1"
                     /translation="MTGSQVVAGHHGMPALSDIDFDKTPFTVAWEITEACGLACVHCR
                     ASAQPKRDPRELTTEEGYALLDGIKEMGNPILVVTGGDPLMRRDVFDLIRYGAGKGLR
                     VSLSPSATGLLNEKNLQKAKDAGVVRIHLSLDGSTPEIHDAFRQVHGSFARTLKGIEI
                     AKEIGMSLQIGTTVTRRNLEDLPALAELMVEFGALMWNIFFLVPTGRGKESDMITPEQ
                     HEATFQWMYDLSKSAPYDVRTTAAQHYRRVVFERTRAELGEKGAIHQALSGAGYSFQD
                     GLGRPQQGVNDGKGFCFISHIGDVCPSGFLQLPAGNVREQPIYEIYRNSKLFKELRDP
                     SLLKGRCGRCEYRRVCGGSRARGWALTGDYLAEEICCTYEPQSGHVL"
     gene            complement(4893..5435)
                     /locus_tag="VHS06_02605"
     CDS             complement(4893..5435)
                     /locus_tag="VHS06_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HEX2987043.1"
                     /translation="MLKESVHEGLEKHDEITIGKHGVLTVGGARAVTLTQEAYAFMMQ
                     VLHEHAPHAIKYAFYDMGYQVGRQLMAATMTEDRDPENGFRLLVNSYKQLGYGDLQVD
                     HFNMDGPEAHLSGINLFETGVAQISGVYRTPRCVDHYSRGMFAGFLSDLLKREVVCEE
                     VACTYRGDPRCEFVVLPYER"
     gene            complement(5505..>5883)
                     /locus_tag="VHS06_02610"
     CDS             complement(5505..>5883)
                     /locus_tag="VHS06_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX2987044.1"
                     /translation="LFLYEKRMVEMAAGRPVRIVADSISTLLSTASATDILEFSGHRL
                     RYLRSRQVLTLDVYVGGVMEERAVAGLAHLYPMILRMHYQSTGNVLQRYLQLGKLKSG
                     QFTSQQHQFNIDSKTGIIVQPDR"
BASE COUNT         1074 a         1723 c         1905 g         1181 t
ORIGIN      
        1 gcgttggagg gatcgcacac cctgtaggtg aagttagctg tgccgttgaa gtcgccggga
       61 ctgtaggtga aggagccgtc gctgccgagg ctgagagtcc cgccggcggg gccggccacc
      121 agcgacaccg tcagcgggtc gctgtcggcg tcgctgtcgt tgtgtagcac accaaacgcg
      181 ccgttataaa caagagtcgc gttctccagc atagagggga aagagtcgtt taccgctatc
      241 ggcggatctt gtaccggggt gatggtgatg ctcaccgtgg cagtatcgaa gtcggggctg
      301 ccgtcgccaa tcttgtatgt gaaggagtcg gtaccattgt agttgggggt cggcgtgtag
      361 ttgaaggagc cgttggcgtt gagtgccagc gtcccgtgag aagggccgga ctgcagtgat
      421 atgtccagcg cgtcgccatc agggtcggag tcattggcca gcacaccggg ggcggacacc
      481 gacaatgtgg tatcctcggc cgtggtatag gattcaccag tcgcaatggg cgcactgttg
      541 gtcggaattc ccagctgcgc tccagacagc acccagaggg ggccgttcac cagggtgccg
      601 tcccggccat cggcggcact agaatcgaca gcagtagtgc cggagccctt gtcgaactgc
      661 caggctccca gcaaaccgat ctccctctgc cgggttcgaa ttgcgtaaga ggcggcgatt
      721 tgctccggcg tgcgggccac gccccagaac cggacttcat ccatggtgcc ctggaagtag
      781 cctgagctcc acgctccgct gccgaccgcg cggcccaccg cctgatacca tccggtagtc
      841 cattgggggg gccacatccc ccactgccac cggggtgccc tgctccacgc cgttcacgta
      901 gagccgcatg ctccccccgt cttcagctac tgctgccacg tggtaccagg tgttcggtac
      961 cacgacggtg ctgccggtta ccaccttggg gattccatta tcggtgagat agtgctcgaa
     1021 ccggccactg ctgttgatcc gtagaaggtt ggtgaactga gacgtttcct ggccggctga
     1081 ggccgcggcc atcagattct gtgcagcggt gctggatggc ttcacccaac cctccagggt
     1141 gtacgcggtc tggtggcctc ctggcggcag cggcacgtta aggtagtcgt ctgccccatc
     1201 gaactgaagt gcgtagccgg aaatgccggc ccagtagtgg gcagctatcc ggtcggcgga
     1261 gaggactttg cggtagaaag acacctcgtc catcaggccg ttgaagtagt cctggttggc
     1321 cattccgtag ttccccaggg agacaaagcc gaagccggcg gtcacacctg taagcgagaa
     1381 attgcctgtt acggggtctg cttgcccgtt gatgtaggtt tgcgtggttt tggtcgcgcc
     1441 gtcgtaagtg aacgccaggt gggtccactg atcggcgggg atcgagcctg cagatgtcga
     1501 gcaattccaa atgccagtgg gggcagtgcc ggtgccgctg cctgggcaga actcggcttt
     1561 gcctgcattg tcgagtatct tgacgctgaa cgtatccgga tctttagtct tcgcgaagag
     1621 ggtgccggtc tgcgtggtgt tggcgggttt aacccacatc tccacgctga aggagtcgcc
     1681 gggcgcaagc tgcaggtctg tggaattatt gatccgcagg tagctgctgg atccattgaa
     1741 ggaggcggcg gaatccgggt cgccggccag aatcccgggc tgaagggcca cgttactgga
     1801 ggtgcttatc acgtccggga cggagccacc gttgccttcg tccggggccc ctacgccaac
     1861 ctgtcccaac cgccaccaac cgtaaggacc atctgcctgg acggcggtgg tgtatgcaga
     1921 gggttgggtg atggattgga tgccgaagct gcgctgaatc gttcgcagcc gcactgacgt
     1981 ggcggttgga gccatggctg tagccgtcgc agtcgcggag gtgacggtag ccgtcgccgt
     2041 agaggctgcg atcgttggcg tcgccttggg agtagtagca gtgattgtgg cggtagtcgc
     2101 gggtgccatg ccggccgtgg ggcttccgct gggaatggcg ccgggagaca cggaaggcac
     2161 ggcttccacg gtggatgtag aagtcgccgt gatggtgggg gtggcagtat cagggccggc
     2221 gctcgttgta ggcgaaggcc ccagaatctc cgggctgagc gatgcggccg tgccgacgcc
     2281 ggctgaggct atcagcaagc agagtagcgg caccaccatg agggagaaga ctcgggagcc
     2341 gtcgtggctg ttcatctggg acctcctcga cgttacggag tccgtgaaat gcgcctaacc
     2401 aagtcaccag gtcgccacga ttcgcaaaga tgagtcgttc gtgtggcctt gccccggatc
     2461 catcctggtt gccagatagg ctcttaagtg ctgggccgcg ctgttttcgc acgcagcaca
     2521 tgtcttggta aggctcatgg acatgacgca agttggcgca gcatcgcgcc gtgttgtcac
     2581 ccttggtccg aagggtggag ttgtctgccc ggcctcattc agcgctagga ggagcgcgac
     2641 ccacatgggt gctctccaat gcacaggggg aaaggcctcc tgtcagagcg ccagggcaac
     2701 tgacagccca gtacgggagt agggaggatc aagctgctgg cgtctccggg ggaagatacg
     2761 ccacggcccc tacgagagca gaaactacaa ctaagtatag actactgcct tctgtcgggc
     2821 aacacgtgtc gcaacaacag gcggggccgc tgcatcgatg agactgaccg ggcattgtga
     2881 gcccgaccga caacccaaca ccaaatcacc aggtaaccta ttgtttacgg caacggccag
     2941 cggaacaccc gctcacgcac ctgctggagg tcgcggataa cgaactcgat gtagcgtgcg
     3001 ccgtcccgcg gcaccggccg cgcccgcgca tcggtgcgtg agaaggccgc cactccgctc
     3061 agggtgccgc tacccgctgt cggtacccat gtgggggtcc cgtactcctt gccaccgtcc
     3121 tctcgaaggt acaacatccc tcgcaggtcc cagacagata ttttcgatcc cggtgcgtct
     3181 accaccactc gaaagatcag ctgtttctgg ggatcgaacc cctgaccggc ggctgggtct
     3241 tgcttgaggt agtctgcggt gagccaggtc accgtgacct tggcgccgct gctaccgtcg
     3301 tcggagcggg catactggcg ctgtagccct tccaacgctg ccggaagggg cgtgggtgtg
     3361 atggtgggaa ctggggttgg cgtcgcctct ttggcgacgc tctgagcagc cggcccgcag
     3421 ttggccacgg caaaaagggg gagtgccagg gcggcaacga gcgtgacaat gtaacgacct
     3481 gcttggcggg tgagcaagga ttggtctccg tgaaagggtt gatgggctgt cgtgaatgat
     3541 accctagtct attatatggc ggcgttgcgg gccacccaaa tgcccggatg gtagttgctg
     3601 gaccacgact gctttacggg gtaaactaag tagccaatat cataaatccc tctcttggtg
     3661 tcaatctggt ggctggaggc aaagatgacg ggttcgcagg tggttgcagg ccatcacggc
     3721 atgcctgccc tgagcgatat agacttcgat aagactccct tcacggtggc ctgggagatc
     3781 accgaggcat gtggcctggc ctgcgttcac tgtcgggcat cggctcagcc gaagcgcgat
     3841 cctcgggagt tgaccacgga ggagggttat gcccttctcg acgggatcaa ggagatgggc
     3901 aacccgatcc ttgtggtcac cggaggagac cctctgatgc gccgcgacgt cttcgacctg
     3961 atccgctatg gggcgggcaa ggggttacgg gtctccctct cgccctcggc cacggggctt
     4021 ctgaacgaga agaacctcca gaaggcgaag gatgcgggcg tggtccggat tcaccttagc
     4081 ctggatggct ccaccccgga gatccacgac gctttccggc aggtgcacgg ctccttcgct
     4141 cgcactctca aggggatcga gatcgccaag gagatcggca tgtcgctcca gatcggcacc
     4201 accgttaccc gccggaacct ggaggatctg cctgccctag ccgagttgat ggtagagttt
     4261 ggcgcactta tgtggaacat cttcttcctg gtccctaccg ggcgcgggaa ggagtcggac
     4321 atgatcaccc ccgagcagca tgaagcgacc tttcaatgga tgtacgacct ttccaagagc
     4381 gcgccctacg acgtccggac caccgcggcg cagcactatc gaagggtggt gttcgagcgg
     4441 acgagggcgg agttgggaga gaagggggcg atacaccagg cgctcagcgg cgccggctac
     4501 agcttccagg atgggctggg gcgtccgcag cagggggtca acgatgggaa gggcttctgc
     4561 ttcatctccc acatcggcga tgtgtgtcct tccggtttcc tccagctacc ggcaggcaac
     4621 gtgcgggagc agccgatcta cgagatctat cgcaactcca agctgttcaa ggagctgcgt
     4681 gacccctctc tcctgaaggg tcggtgtgga cgctgcgagt atcgaagggt gtgcggcggg
     4741 agtcgagccc gcggctgggc tctcaccggc gactatctgg cagaggagat ctgctgcacc
     4801 tatgagcctc agagcggtca cgtcctctga ttcgtctcgt agggcagcat agatacccgc
     4861 ggtgttgcag acaccgcggg gcgaattacc ccctacctct cgtagggcag caccacgaac
     4921 tcgcagcggg ggtctccccg gtaggtgcag gcgacctcct cgcaaaccac ttcccgcttc
     4981 aagagatccg aaaggaagcc cgcgaacatc ccccggctgt agtgatccac acagcggggc
     5041 gtgcggtaca cccctgatat ttgggctacc ccagtctcga agaggttaat gccggagaga
     5101 tgagcctccg gtccgtccat gttgaagtgg tcgacctgca gatccccgta tcccagttgc
     5161 ttgtagctgt ttaccaggag gcggaagcca ttttccgggt ccctgtcttc tgtcatcgtt
     5221 gccgccatca actgtctgcc aacctggtac cccatgtcgt agaaggcgta cttgattgcg
     5281 tggggggcgt gctcgtgcaa tacctgcatc atgaaagcgt aggcttcctg ggtcagcgtc
     5341 actgcccgcg ccccaccgac tgtgagcacg ccgtgcttgc cgattgtgat ctcgtcgtgc
     5401 ttctccaaac cttcgtggac gctttccttt agcactgcgg ccctccgtcc ggcatttgcc
     5461 gtcccgactc ccggccccta acccactgtc actcaggcct ggagtcagcg gtccggttgg
     5521 actatgatcc ccgttttgct gtcgatgttg aactgatgtt gctggctggt gaactgcccg
     5581 cttttcagtt tgcccaactg cagatatcgc tggaggacgt tgcccgtgga ctggtagtgc
     5641 atcctcaaga tcattgggta taggtgggcc aggccggcca cagcccgctc ctccataacc
     5701 ccgccgacgt acacgtccag agtcagcacc tgccgggatc gcagatagcg cagcctgtgt
     5761 ccgctgaact ccaggatgtc ggtggcgctg gccgtggaga ggagggtgga aatggaatcc
     5821 gcgactatcc tcaccggcct tccggctgcc atctctacca tccgtttctc gtagagaaag
     5881 agt
//
LOCUS       DATALS010000122         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_236741, whole genome shotgun sequence.
ACCESSION   DATALS010000122 DATALS010000000
VERSION     DATALS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236741"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VHS06_02615"
     CDS             complement(<1..209)
                     /locus_tag="VHS06_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HEX2987045.1"
                     /translation="MNAVNIDRTVGELVAEQPGRARVFERLGIDYCCGGKRPLKVVCE
                     EKNLPVEAVVGQLASEASPGQAETN"
     gene            345..794
                     /locus_tag="VHS06_02620"
     CDS             345..794
                     /locus_tag="VHS06_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX2987046.1"
                     /translation="MISQTAEYALRAMTCLAGEYNKPLTTQQIADAAHIPVGYLYKVL
                     QSLGRAGLVQSTRGLHGGYVLSLPPEDISILRVVQAVDPIQRIKVCPLNLKSHSTRLC
                     PLHRRLDEAAAMVEHAFAQTTLAELLHPGPGAFPRPLCDCGHSGKKK"
     gene            841..>1671
                     /locus_tag="VHS06_02625"
     CDS             841..>1671
                     /locus_tag="VHS06_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987047.1"
                     /translation="MIAQAHESTDSLDIHPEMLLPELFERYPTTRQVFDRYGLSGCGG
                     HGGPVESIRFFARAHGVDESSLLAELRHATHAPQPSTPLAAPSLADSIYRRFFVGGIL
                     IVLTFGATWGAWLLWQIAFSHSFLGANSIQDVNAHGHAQIYGWVGLFIMGFAYQAFPR
                     IWHTRLPVPRLAVLVFSAMASGIILRCIGMELHGLSNTALPLALLGGFLEIAAVLTFA
                     VHILSTFRASGARLEPYVGFVIVALISFFAMSLLDVWHTYTTMSAQRLMELLWYIATY
                     Q"
BASE COUNT          286 a          625 c          416 g          344 t
ORIGIN      
        1 caattcgtct ccgcctgccc cggggatgct tccgaggcca gctgcccaac aaccgcctcc
       61 acggggaggt tcttctcttc gcaaaccacc ttgagcggac gcttcccgcc acagcagtag
      121 tcaatcccca gtcgctcaaa gacgcgggcc cgccccggtt gctccgccac caactctcca
      181 accgtcctat cgatattcac agcgttcatt gctctgatcc tttcacggca accacacacc
      241 ggatatctgt acctaaatat ccaattgacg aaagaaccat aggccctata atctggatgt
      301 caagatccac ttatccaaat tgaaatgcgt tcttaggagt tgccgtgatc tcacagaccg
      361 ctgaatacgc cttgcgggcg atgacctgcc tggcaggcga atacaacaaa cccctgacca
      421 cccagcagat cgccgacgct gcccacattc ccgtcggcta cctctacaag gtcctccaat
      481 cgctgggccg agcgggtctg gtgcagtcca cccggggcct gcatggcggc tatgtcctga
      541 gcctgccccc cgaggacatt agcatccttc gcgtggtgca ggccgtcgat cccatccagc
      601 gaatcaaggt ctgccccttg aatctcaaga gccacagcac ccggctctgc cctctgcatc
      661 gccggctcga cgaggcggcc gccatggtcg aacacgcctt tgcccaaacc accctggccg
      721 agctgttgca ccccggcccc ggcgcatttc cccgtcccct gtgtgactgc gggcacagcg
      781 gaaagaagaa atgatcgctt tgccttgtgt ctgccctgac cgaccaaagg agatggcccc
      841 atgatcgccc aggctcacga atcaaccgac tcgctcgata ttcaccccga gatgctcttg
      901 ccggaactgt tcgagcgcta ccccactacc cggcaggtct tcgatcgcta cggcctgagc
      961 ggctgcggcg gccacggcgg acccgtggag tccatccgct tcttcgcccg cgcccatggc
     1021 gtcgatgaat cctccctcct ggccgaattg cgccacgcca ctcatgctcc ccaaccgtcc
     1081 acgcccctcg ccgctccatc cctcgccgac tccatctatc gccgcttctt cgtcggcggc
     1141 attctcatcg tgctcacctt cggcgccacc tggggcgcct ggctcttgtg gcagatcgcc
     1201 ttcagccatt cgttcctcgg ggccaactcc attcaggacg tcaacgccca tggccacgcc
     1261 cagatctatg gctgggtggg cctcttcatc atgggcttcg cctaccaggc gtttccgcgc
     1321 atctggcata cccggctgcc cgttccgcgc ctcgcggtgc tcgtcttctc cgccatggcg
     1381 tcgggcatca tccttcgctg catcggcatg gagctccatg gcctgagcaa caccgccctg
     1441 ccactggctt tgctgggcgg attcctggag atcgccgccg tcctcacgtt cgccgttcac
     1501 atcctctcca ccttccgcgc cagcggcgcg cgcctggagc cgtatgtggg cttcgtgatc
     1561 gtcgcgctga tctccttctt cgccatgagc ctcctggatg tctggcacac ctacaccacc
     1621 atgagcgccc agcggctgat ggaactcctt tggtacatcg ccacctatca g
//
LOCUS       DATALS010000123         3905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_191680, whole genome shotgun sequence.
ACCESSION   DATALS010000123 DATALS010000000
VERSION     DATALS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191680"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VHS06_02630"
     CDS             complement(<1..233)
                     /locus_tag="VHS06_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2987048.1"
                     /translation="MAVQHVIVIGGGSTGCACAHDLVLRGFEVTLVERGELASGTTGR
                     CSCLLHSGARYCVKDQESALEAIEENKIMRRVLP"
     gene            complement(442..1938)
                     /gene="glpK"
                     /locus_tag="VHS06_02635"
     CDS             complement(442..1938)
                     /gene="glpK"
                     /locus_tag="VHS06_02635"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX2987049.1"
                     /translation="MAEKYILAIDEGTTGTRALIIDHNSNIKAQAYSEFTQYTPTPDR
                     TEHDPEEIWNATYAMMKKALAAADLKPTDIATIGITNQRSTRMAWNRYTGKPIYPAIV
                     WQDMRNQAFVESIRPQWGEKAYKHTGWPLCPLYSSIGLWWMMENVPGARAQAEAGDVL
                     FGNVDVWLIYKLTGGKVHATSASNATVAGYYDLYRDEWYKEWLDFLGLPLHAFPEVRD
                     DGGFFGVTDPSLFGAEIPITGDIGDQQAGLFGQGGLTPGDVKCTHGTGTFLNMNVGDR
                     LPDSRSGTNVLIAWRMNGKTTYTLEGYASVTGSAVQWLRDGAELIKSSAETEPLAASV
                     ADNGGVYFVPAMTGLAAPWWDSSARGMMIGITRGTSRTHLARATLESIVYQTKDFLQS
                     MHQDSGMEIKSMRVDGGAVRNNWLMQFQADMLDAEVIRPVNPEATVMGAAYLAGLACG
                     YWSSPEEAFAGLKIDCTFQPKMPAGERERLYGEWTRAVHHAMGWLPNQ"
     gene            complement(2123..2500)
                     /locus_tag="VHS06_02640"
     CDS             complement(2123..2500)
                     /locus_tag="VHS06_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987050.1"
                     /translation="MEQTVSGKPVQRADLSSLGWLVQAVSGLLLVVLLLLHMIANHFV
                     AAGGLQSFQDVVRYLSNPLILVLETAFLVTVTSHALLGVRAVLFDLNLSAGAKRAVTA
                     LLTVLGVAMVGYGVWLTSIILRS"
     gene            complement(2532..2942)
                     /gene="sdhC"
                     /locus_tag="VHS06_02645"
     CDS             complement(2532..2942)
                     /gene="sdhC"
                     /locus_tag="VHS06_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX2987051.1"
                     /translation="MSVEDMGGKRDGMPTGQGIVNWFDPRRRGVGMWAFVLNRLSGLG
                     LAVYLILHLAVLSTLARGEANWEAFLVLARSPVLLLLDSALFAALLYHGLNGIRVALV
                     GMGFGVERHKVSFGVVMGLAAAALVYAVMRLWTA"
     gene            complement(2949..3689)
                     /locus_tag="VHS06_02650"
     CDS             complement(2949..3689)
                     /locus_tag="VHS06_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX2987052.1"
                     /translation="MSWRVNFRIFRYKQDGSAAHYDAFALDVRPDEYVLDGVERIWAE
                     HDRTLVFRHACHHAGCGACAMRVNGRERLTCITRIDAVTQDGGTVTVEPLRNLPVVSD
                     LLVDMSPFYSKLELAQFVPVRQAEPMIDLASQQVAADCQPAMRFENCLECGICVSACP
                     AAGTDPDYLGPAALAAIARMVAEPRGKVDPAGLFDLADCEQGLWRCHTAFECTEACPG
                     NVQPGTLIMALRRQALSRAIKRLFGARS"
     gene            complement(3710..>3905)
                     /locus_tag="VHS06_02655"
     CDS             complement(3710..>3905)
                     /locus_tag="VHS06_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2987053.1"
                     /translation="RTFKCFDHAESDDVQGFVTITGGKGTTSRAMAEATSDVVCHKLG
                     VTAPCRTREVVLASYRTFYQ"
BASE COUNT          814 a         1324 c         1070 g          697 t
ORIGIN      
        1 ggcaagacgc gccgcatgat cttgttttcc tcgatcgctt ccagcgccga ttcctgatct
       61 ttgacacagt aacgggcacc gctatgcagc aaacaactgc agcgccccgt ggttcccgac
      121 gccagctcac cgcgttcgac aagtgtcact tcaaaaccgc gcagcacgag gtcatgtgcg
      181 caagcacagc ccgtagagcc acccccaatg acaatcacgt gttgtactgc catactctgc
      241 tctcccgcaa acccctccgg cagccacacc taagcccacc cggcccgtgt ggcgaatgcc
      301 tggaatcgcg cgcggaaacc gacaaatcag cccataccac catctccgac ccgatcatta
      361 tcgattgggt cggagatgaa catgcaccta cgacaccact cccagcatca gcgactccac
      421 cgcaggctgt tttcgtagag attattgatt agggagccag cccatggcat ggtgtacggc
      481 ccgggtccac tccccgtaca gacgctcgcg ctcgcccgcc ggcatcttcg gctggaatgt
      541 gcaatcgatc ttcagcccgg cgaaggcttc ttcaggagaa ctccagtaac cacaggccag
      601 gccggccaga taagcggcgc ccatcactgt ggcctctggg ttcactggcc ggatcacttc
      661 agcatccagc atatcggcct ggaactgcat cagccagtta ttgcgcaccg caccgccgtc
      721 cacgcgcata ctcttgatct ccatgcccga gtcctggtgc atgctctgca ggaaatcctt
      781 ggtttggtag acaatcgatt ccagcgtagc gcgcgccagg tgggtacgtg aggtaccgcg
      841 ggtgatgccg atcatcatgc cgcgcgccga ggaatcccac cacggagcag cgaggccggt
      901 catcgccggc acaaaataga cgccgccatt atccgcgaca ctggcggcca ggggttcggt
      961 ctcagctgag gacttgatca gttcggcacc gtctcgcagc cactgcacgg ccgaaccggt
     1021 gaccgaggca tagccttcca aggtataggt agtcttgcca ttcatgcgcc aggcaatcag
     1081 cacgttggtg ccgctgcggg aatcgggaag tctgtcaccg acattcatat tgaggaaagt
     1141 gcccgtgccg tgcgtgcact tgacatcgcc gggggtcagg ccgccctgac caaagaggcc
     1201 tgcctgctgg tcgccgatgt cgcccgtgat agggatctcg gcaccaaaga gggagggatc
     1261 ggtgacaccg aagaagccac cgtcgtcccg cacctcgggg aaggcatgga gcggcagccc
     1321 caggaagtca agccattcct tgtaccactc gtcacggtac aggtcataat agcccgcgac
     1381 cgtggcattg gaggccgacg tcgcatggac cttgccaccg gtcagcttgt agatcagcca
     1441 tacatcgaca tttccaaaga ggacatcgcc ggcttcggcc tgcgctcgcg cgccaggcac
     1501 attctccatc atccaccaca agccgatcga cgaatagagc gggcaaaggg gccagcctgt
     1561 atgtttgtag gccttctcgc cccactgcgg cctaatggat tcgacaaagg cctgattgcg
     1621 catatcctgc cagacgatag ccggatagat cggcttgccg gtgtaccggt tccaagccat
     1681 acgagttgag cgctggttgg tgatgccgat cgtcgcaata tcggtaggct tcaagtcggc
     1741 agcggccagc gccttcttca tcatggcata ggttgcgttc cagatttcct ccggatcatg
     1801 ttcggtgcgg tccggagtcg gggtatattg ggtgaactcg ctataggcct gggctttgat
     1861 attgctgttg tgatcgatga tcaacgcccg ggtaccggtc gtgccttcgt caatcgccag
     1921 gatgtacttt tcagccatgg aagtgtcccc tcctgatacg tgaaccctaa actcaatcca
     1981 gagtgggata tacaggatcc gaggattaac ggccgaacgt atagtttctc catgacggaa
     2041 ttcgcctaga ttactgccct accccttcgc agccagatgt tgctcgctaa ggctacctcc
     2101 tctccgatcg gcggtcggag cctcagctcc ggaggatgat cgacgtgagc caaacaccat
     2161 agcccaccat ggccacccct aagacggtca gtagcgctgt gaccgcccgt ttggcccctg
     2221 ccgagaggtt gagatcgaac aagacggccc ggacgcccag cagcgcatgg ctcgtcaccg
     2281 tcaccaaaaa cgcagtttcc agcaccagga tcagcggatt ggacaggtac cgcaccacat
     2341 cctggaagct ctgcagcccc ccggctgcca cgaaatggtt ggcaatcatg tgaagcagca
     2401 aaagcaccac cagcagcaga cccgataccg cctgcaccaa ccaacccaga ctgctcaggt
     2461 cagctcgctg gaccggcttg ccggacacgg tttgctccat cgcctgtctc catccttctc
     2521 ccggtgcccc tctaggccgt ccagaggcgc atcacggcgt agaccaaagc cgccgcagcc
     2581 agccccatca ccaccccaaa ggagaccttg tggcgctcca ccccaaaccc catcccgacc
     2641 aacgccaccc gtatgccgtt caagccgtgg tacaacaggg ccgcgaagag ggcgctgtcc
     2701 agcagcagca ggacgggcga acgcgccagc accagaaagg cctcccagtt cgcctcaccg
     2761 cgggccaagg tactcagcac agccaggtgg aggatcagat acaccgccag ccccaagccc
     2821 gacagtcggt tgaggacgaa ggcccacatc cccacgcctc gtcgccgggg atcaaaccag
     2881 ttgacgatcc cctggcctgt cggcatgcca tcgcgcttgc cgcccatatc ctcgacgctc
     2941 atcgtgcact aactccttgc gccaaacagc cgtttgatcg cccgggagag cgcctggcgg
     3001 cgcagagcca tgatcagcgt gccaggctgg acgttgccgg gacaggcctc ggtgcactca
     3061 aaggccgtat ggcaccgcca cagcccctgc tcacagtccg ccagatcgaa cagccccgcc
     3121 ggatcaacct tgccccgcgg ctccgccacc atccgggcga tggccgccag cgccgccggc
     3181 cccagatagt ccgggtcggt ccctgccgcc ggacaggccg agacacagat gccgcattcc
     3241 aggcagttct caaaccgcat cgctggctgg cagtccgccg ccacctgttg cgaggccagg
     3301 tcgatcatgg gctccgcctg gcgcaccggc acgaactgcg ccagttccag cttcgagtag
     3361 aagggtgaca tgtctaccag cagatcgctc accaccggca ggttgcgcaa aggttcgacc
     3421 gtgaccgtcc ccccatcctg ggtcacagcg tcgatgcggg tgatacaggt cagccgctca
     3481 cgtccattga cacgcatcgc acaggccccg caccccgcat ggtggcaggc atgccgaaac
     3541 accagcgtgc gatcgtgctc ggcccagatc cgctccacgc catccagtac gtactcatcc
     3601 gggcgcacat ccagggcgaa ggcgtcatag tgtgccgcag agccatcttg tttgtaacga
     3661 aagatacgaa agttgacccg ccaactcata gccccgtccc ctcctcgatt cactggtaga
     3721 aggtgcggta ggaagccagc actacctcac gcgtccgaca gggagccgtg actccaagct
     3781 tgtggcacac cacatcgctg gtcgcttcgg ccatggcccg ggaagtcgtc cccttgcccc
     3841 ccgtaatcgt cacaaacccc tggacatcat cactttccgc atgatcgaag catttgaagg
     3901 tccgc
//
LOCUS       DATALS010000124         6851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_320486, whole genome shotgun sequence.
ACCESSION   DATALS010000124 DATALS010000000
VERSION     DATALS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320486"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VHS06_02660"
     CDS             <1..941
                     /locus_tag="VHS06_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX2987054.1"
                     /translation="RHFYPQERDATLLSEALLAFQREYQWDFVKVNPRGSYHVEDWGN
                     RYSYSNDPHVAPTLLSHVVHSREDWGKIQRLDIWKSEALKQQLVLLELVKEGLQNENV
                     YFLQTIFSPLAIAFKLAGSSKDRLQEAMKSESKELKAALEAIAESYVDYATASLNVGA
                     SGIFFATTKMASAEMMSEEQYAEFGTPYDLRVLAAVQSRLGFNLLHMCEGNIFFDRLM
                     GYPVDAISWDVTLPGNPSLRKGRERSGKMVVGGLDSMATFKTGKPDVIDREVTSALQE
                     TGGRGCAIAPGCTFPTMTRGDLLEAALAARERFSGN"
     gene            1271..2188
                     /locus_tag="VHS06_02665"
     CDS             1271..2188
                     /locus_tag="VHS06_02665"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX2987055.1"
                     /translation="MDLAPVVAAIDGYLALDWSKIISMAFVLIATLVALRLLKIAVHG
                     VVRTILERDKEPDREVAQKAKTLSQVVETTGRIIVFTLALLTLLSLAGQDITPLLASA
                     GIAGVAIGFGAQNVIKDWLGGFFILFENQYSVDDVIKVGNYSGLVEKMDLRRTVLRTT
                     DGSVVVIPNGEIRVVTNLTKEWSRVVMDVGVAYEADIDHAMDVLVRTATELAQEEEFA
                     KLIIDAPEMLGVEELGEYQVTIRMLVKTQPLQQWKVARALRARIKKAFDREGISIPYP
                     HQVSITKIQPMEFTEERLVSLASQRVRSE"
     gene            complement(2167..3258)
                     /locus_tag="VHS06_02670"
     CDS             complement(2167..3258)
                     /locus_tag="VHS06_02670"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX2987056.1"
                     /translation="MALLILEGYPFRWLFVALCDVSAANGRTNYGQAKVVSPALQGRG
                     CFALCRVELGRVESILSALRGNKLRLIVVNADDFGISEGVNRGIVEAHRRGILTSTTL
                     MANMPAFEQAVALSRENPGLGVGLHLNLTAGAPIIPTSEVPTLVGEDGRFLLGGVFLR
                     QLTLGRLNLREVEAELGAQIERAQGLGIAPTHLDSHHHIHIHPALQPVAIDLALRYGI
                     GGIRSTVEFGLADGVGQVGMLLRGIREGERETVRGRYLKAVVLSVLGRLLQVRARRAG
                     LATPSYFRGLLLGMAFGPTSLHRLLRSLPDGSTELMCHPGYLDEGLKKETSYSVGRDS
                     ELRALIDPVSSEILSERTVRPARYSDLTR"
     gene            3245..3892
                     /locus_tag="VHS06_02675"
     CDS             3245..3892
                     /locus_tag="VHS06_02675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2987057.1"
                     /translation="MSSAIDQAIHLYDDQPLGIRAFVRCRHLLCPLAEVERRVPRDAR
                     ILDLGCGHGLFSSIMALSSPDRSVAGVDPSLAKIQVASTLMDRLPNASFFQGTAEDVS
                     AAGFDAITILDVLYLLPVPEKLRILQRCYELLRPGGQLILKTNDTHPVWKYRWAWFQE
                     VVMTRIGLTLGHGDLHFLSCKENAGLMKQAGFGEVEITHIPTLLPYPHTLLVSSK"
     gene            3889..4644
                     /locus_tag="VHS06_02680"
     CDS             3889..4644
                     /locus_tag="VHS06_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEX2987058.1"
                     /translation="MKPRLSVIIPAYNEEKVIGNTIAKIASYLEARGDNELIIVDDGS
                     RDSTVEIVKGLMAKHPCLRLVVCERNGGKGQAVRQGVMHSQGEFVLYTDADLVYPIEG
                     ETAFTAALEAGEDVAIGCRSHPETLFALNPRHFPYIYQRYLVGRIYILIANSLLKLDV
                     KDTQCGFKLFRGDVARDIFSRVRNYGFAFDVEVIYIARYLGYRVVEMPVYFLYLGEQS
                     SVELARDSIRMLRGLWSIRTNGRLGVYAEPSPQ"
     gene            complement(4712..5104)
                     /locus_tag="VHS06_02685"
     CDS             complement(4712..5104)
                     /locus_tag="VHS06_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2987059.1"
                     /translation="MEGKTNAHKQPESERFCPHYHHAVELIGRRWTGTILRAMLSGLE
                     RFSDISAAIPGLSDRMLSERLKELETEGIAERVVIPETPVRIEYHLTEKGQSLGSVVE
                     AVSDWAHRWAGPEEDAEAKEECGAQGGD"
     gene            5306..5869
                     /locus_tag="VHS06_02690"
     CDS             5306..5869
                     /locus_tag="VHS06_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX2987060.1"
                     /translation="MAADSEATVENATRWVIDPAHSSVEFAVKHMVIATVRGRFTQFD
                     VQLEADEEQPLQSKVVASIDIASIDTRNADRDAHLRSADFFDLENHPQLRFVSRYIEP
                     TGENSGRIVGDLTIRGVTREVALDTTFTGRAKSPWGQEVAGFSAETAINRKDFGLTWN
                     RSLETGGVLVADEIKIFIEIEAIRQQG"
     gene            complement(6057..6722)
                     /locus_tag="VHS06_02695"
     CDS             complement(6057..6722)
                     /locus_tag="VHS06_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2987061.1"
                     /translation="MTFHGAAEPLIRHGHARRVEAAECLELLQQAYDHKLVQFGENVR
                     EGVSFICNCCGCCCEAMLAAKRFGMLHPVHTTNFLPAVDADLCSGCGSCTVVCPVNAM
                     QLVAAGEQGRLERKRAQPDETTCLGCGVCVRSCPSQGIRLESRAERVVTPVNSVHRIV
                     AMAIERGKLQNLIFDEQAMASHRAMAAILGVILKLPPIQQLMASRQMKSRYLEALLRR
                     IKV"
BASE COUNT         1298 a         2170 c         2085 g         1298 t
ORIGIN      
        1 ggcgccactt ctacccgcag gaaagggatg cgacgctgct gtcggaggcc ctgctcgcct
       61 tccagcggga gtaccagtgg gacttcgtta aggtgaaccc ccggggctcc taccacgtgg
      121 aggattgggg caaccgctac agctactcca acgatcccca tgtggctcct accctgctca
      181 gtcacgtggt acattccagg gaggattggg gcaagattca gcgactggat atctggaagt
      241 ctgaggcgtt gaagcagcag ctggtgctgc tggagttggt taaggagggg ctacagaatg
      301 agaatgtcta cttcctccag accatcttct cgccccttgc cattgccttc aagctggccg
      361 gcagctcgaa ggatcgactt caggaggcga tgaaaagcga gtccaaggag ctgaaggccg
      421 ctctggaggc gatagccgag agctatgtag actacgccac cgcatccctc aatgtggggg
      481 ccagcggcat cttcttcgcc accaccaaga tggcctccgc agagatgatg tctgaagagc
      541 agtacgctga gtttgggacg ccctacgacc tgcgggtgct cgcggccgtg cagtccaggc
      601 tgggtttcaa cctgctccat atgtgtgaag gcaacatctt cttcgatcgg ttgatgggat
      661 atcccgtcga tgcaatcagt tgggatgtga cgctaccggg caatcccagc ctcagaaagg
      721 gccgtgagag gtcgggcaag atggttgtgg gtggcctgga cagcatggcg accttcaaga
      781 ccggcaagcc agatgtcatc gacagggagg taaccagtgc gctccaggag acgggtggcc
      841 gaggctgcgc aattgcccca ggctgcacct tccccaccat gactcgtggc gacctgctgg
      901 aggcggccct ggccgcaagg gagcgattca gcggcaacta gctgtcggcc atcagcagtc
      961 agctgtcagc tagtggtcct tggctgacag ccgccctccg aagccgccgg gtatacccac
     1021 ggcggccggt atcagtttgc cctccagccg gtatgtgtta cgattctatt ctatggtcta
     1081 ccgtcggaat ctcgccggct cttcgtggtc ttgtgcgacg tctcggtcgt aaacggccgg
     1141 gctgactacg gtcaggccaa cgcggttagc ccggcccttc agggccgaga cgatcccttc
     1201 tatcgagaag ctgattgctg acagctgata gccgaccgct gattgctgat agctatcccg
     1261 gaggtctaca ttggatcttg cccctgtcgt agccgcaatc gatggctacc tcgctctgga
     1321 ctggtcgaag atcatctcca tggcgttcgt tctcatcgct acgcttgttg ccctgaggct
     1381 tctcaagatt gcggtgcatg gcgtggtgcg gaccatcctg gagcgggaca aggaaccaga
     1441 tcgggaggtt gcccagaagg ccaagaccct ctctcaggtg gtggagacga cgggcaggat
     1501 catcgtcttc acccttgcgc tgctcaccct gctctccttg gccgggcagg acatcacccc
     1561 tctcctggct agcgccggta tcgctggtgt tgccataggt tttggggcgc agaacgtgat
     1621 caaggactgg ctcggcggct tcttcatcct gttcgagaac cagtactcgg tggatgacgt
     1681 gatcaaggtg ggcaactact ccggcctcgt ggagaagatg gatctgaggc ggaccgtgct
     1741 gcgcaccacg gatggctcgg tggtggtgat tcccaacggc gagatacggg tggtgaccaa
     1801 cctgaccaag gagtggtcca gggtggtgat ggacgtgggc gtcgcctacg aagcggatat
     1861 cgaccacgcc atggatgtgc tcgttcggac tgccacggag ctagcccagg aggaggagtt
     1921 tgccaagctg atcatcgatg cccctgagat gctgggggtc gaggagttgg gggagtacca
     1981 ggtcaccatt cgtatgctgg tgaagactca gcccctgcag cagtggaagg tggcgcgggc
     2041 acttcgggcc aggatcaaga aggcgttcga ccgagagggg atctccattc catatcccca
     2101 ccaggtcagc attaccaaga tacagccgat ggagttcacc gaggagcggc tggtcagcct
     2161 cgccagtcag cgggtgagat cggagtagcg ggcgggccgc acggtcctct cgctcaggat
     2221 ctcgctgctg accgggtcga tcagagccct gagctccgaa tccctcccca ccgaatagga
     2281 ggtctccttc ttcagcccct catccaggta acccgggtgg cacatcagct ccgtgctccc
     2341 atccggcagg gaccgaagca gcctgtggag tgaggtgggg ccgaaggcca tccccaacag
     2401 gagaccccgg aaatagcttg gcgtcgccag cccggctcgt cgagccctca cctgcagcag
     2461 ccggcccagc acgctcagca caacggcctt caggtatctg cccctgaccg tctccctctc
     2521 cccctctcga atcccccgga gcagcattcc cacctgccct accccatcgg ccagcccgaa
     2581 ttcgacggtg gatcggatcc cgccgatccc gtagcggagg gcgaggtcga tcgctactgg
     2641 ctgaagagcc ggatggatgt gtatgtggtg gtgagagtcc aggtgggtag gtgctatccc
     2701 caacccctgc gccctctcta tctgggcgcc cagttcagct tcgacctccc gaaggttcag
     2761 ccttcccagc gtcagctgcc gcagaaacac tccaccaagg aggaaacgcc catcctcgcc
     2821 caccagggtg gggacctcgg aggtggggat gatgggggca ccggcagtca ggttgaggtg
     2881 gagccccacc ccaaggccgg ggttctcgcg cgagagcgcg acggcctgct cgaaggcggg
     2941 catattggcc atcagcgtgg tgctggtcag gatgcctcgc cggtgggcct cgacgatgcc
     3001 cctgtttacc ccctcgctta tgccgaagtc atcggcattg acgacaatca acctcaactt
     3061 gttccccctt agcgcggata gtatactctc gacacgcccc aactcaacgc ggcaaagtgc
     3121 gaaacagcct cgaccctgaa gggcagggct aaccaccttt gcctggccgt agttagtccg
     3181 gccgttcgcg gccgagacat cacacaaagc cacaaagagc catcgaaagg gatatccctc
     3241 cagtatgagc agcgccatag atcaggccat ccacctgtat gatgaccagc ccctcggcat
     3301 ccgagccttc gtccgttgcc ggcacctcct ctgcccactt gccgaggtgg agcggcgcgt
     3361 gcccagggac gcgcgcattc tcgatctagg atgcggccac ggactgttct cctccatcat
     3421 ggccctctcc tcgccggaca ggtctgtggc cggagtagat ccatccctgg ccaagatcca
     3481 ggtggcgagc acgctgatgg acaggctacc gaacgcctcc ttcttccagg ggaccgctga
     3541 ggatgtgtcc gcggccggct tcgacgccat caccatcctc gatgtgctct acctgctgcc
     3601 cgtgcccgag aagctgcgga tcctccagcg ctgctacgag ctgctgcgcc ctgggggtca
     3661 gctgatcctg aagaccaacg atacccatcc cgtctggaag taccgctggg cctggttcca
     3721 ggaggtggtg atgacgcgga ttgggctgac gttgggccac ggcgacctcc acttcctctc
     3781 ctgcaaggag aacgccggct tgatgaagca ggcggggttc ggggaggtgg agatcaccca
     3841 catccccacc ctgctcccct atccgcacac cctgctcgtc tcctccaaat gaaacctaga
     3901 ctgtcggtca tcatccccgc ctacaacgag gagaaggtga ttgggaacac catcgccaag
     3961 atagcctcct acctcgaggc gcgaggtgac aacgagctga tcatcgtgga cgacgggagt
     4021 cgggattcca cggtggagat agtcaagggg ctgatggcga agcacccctg cctgcgcctg
     4081 gtggtgtgcg agcgcaacgg cggcaagggg caggcggtcc ggcagggggt gatgcactct
     4141 cagggagaat tcgtcctcta caccgatgcc gatctggtct acccaatcga gggagagacg
     4201 gccttcacgg ccgcactgga ggcgggagag gacgtggcga tcggctgccg gtcccatccg
     4261 gagaccctct tcgctctcaa ccctcgccac tttccataca tctaccagcg ttacctggtt
     4321 ggccgaatct acatactgat agccaacagc ctcctgaagc tggacgtgaa ggacacgcag
     4381 tgcggcttca agctgttccg gggcgacgtg gcgagggaca tcttcagccg ggtgcgcaac
     4441 tacggcttcg ccttcgacgt cgaggtgatc tacatcgccc gctacctggg ctacagggtc
     4501 gtcgagatgc cggtctactt cctctacctc ggcgagcaga gcagcgtcga gttggcccgc
     4561 gactcgatcc gtatgctccg cggcctctgg tccatccgca cgaacggccg tctcggcgtg
     4621 tacgccgagc cctcgcccca gtagcgccga gaaccgtcca acccgccggt acgggctact
     4681 ccgatccctt gatggggctg catcgccgca gctaatcccc tccctgcgcc ccgcattcct
     4741 ccttagcttc cgcatcctcc tcaggaccgg cccacctgtg ggcccaatcc gacaccgcct
     4801 ccacgaccga gccgagcgat tggcccttct cggtgaggtg gtactcgatg cggacgggcg
     4861 tctcggggat gaccacccgc tcagctatcc cctctgtctc caactccttc aatctctcgg
     4921 atagcatccg atcgctcagg ccaggtatgg ctgcggatat gtcgctgaaa cgctcgaggc
     4981 cgctcagcat ggcgcgcagg atggtgccag tccatcgacg cccaatcagc tccaccgcat
     5041 ggtggtagtg agggcagaaa cgctcggact cgggctgctt gtgtgcgttg gtcttgcctt
     5101 ccatgtcggc aggttgcccc ctggtaatgg tttcacagca ataatagcat aagtcgggca
     5161 cttgacggta gagactaatc attgtatagt acctacgaac cgataaggtt gtcgccgcgc
     5221 gtgagagatg cgggcgacca agtagcacat acagtggccc ggccaaactg aggggttacc
     5281 ggaccaccct aaggagacaa acacgatggc agctgattct gaagcaacag tggaaaacgc
     5341 aaccaggtgg gttatcgacc ctgcccactc cagcgtggag ttcgcggtca agcatatggt
     5401 gatcgccacc gtgcgagggc gcttcaccca gttcgatgtt caactggagg ccgatgagga
     5461 gcagccgctg caatccaagg tcgttgccag catcgacatc gccagcatcg acaccaggaa
     5521 cgcggatcgc gatgcgcatc tgcgctccgc cgacttcttc gatttggaga accatccgca
     5581 gcttcgcttc gtcagccgct atatcgagcc gaccggtgaa aacagcgggc gcatcgtcgg
     5641 ggacctcacg atccgaggcg ttaccaggga ggttgcgctg gataccacct tcaccggtcg
     5701 ggccaagagc ccctggggac aggaggtagc cggcttcagc gccgagacgg ccattaaccg
     5761 gaaggatttc gggctgacct ggaaccgctc ccttgagacc ggcggcgtgc tggtcgccga
     5821 tgagatcaag atattcatcg agatcgaggc gatcaggcag cagggctaac gcagggtgcc
     5881 gcataccggc ttcaacggcg cgtccctacc tgtcattctg agtggcccgc aggccacgaa
     5941 gaatctcctg ttgacggagt acccggagat tcttcggcgc tgcggcgcct cagaatgaca
     6001 gggacgggcc cctcagaatg gcatggggcg gcttgccccc ccccccgcct gcagggctat
     6061 accttgatgc ggcgaagcaa agcttccaga tatctggact tcatctgcct gctggccatc
     6121 agctgctgga ttggcggcag cttcaggatc actcccagga tcgctgccat cgcacggtgg
     6181 ctcgccatcg cctgctcatc gaagatcagg ttctgtagct tccccctctc tattgccatc
     6241 gcgacgatgc gatgcacgga gttgaccgga gtgaccaccc gctccgccct cgactccagc
     6301 cttatgccct ggctggggca ggatcgcacg cacaccccgc atccaaggca ggtagtctcg
     6361 tccggctgcg cacgtttgcg ttccagcctt ccctgctcac cggcggcgac caactgcatt
     6421 gcgttgaccg ggcagaccac cgtgcagctg ccacaaccgc tgcacagatc ggcatcgacg
     6481 gccgggagga agttggtggt gtggaccggg tggagcatcc caaaccgttt ggccgcgagc
     6541 atcgcctcgc agcagcagcc acagcagttg cagatgaagc tgactccctc ccgcacgttc
     6601 tccccaaact gcaccagctt gtggtcgtag gcctgctgaa gcagttccaa acactccgcc
     6661 gcttcgaccc tgcgggcatg cccgtgccgt atcagcggct cggccgcccc gtggaaggtc
     6721 atgcagatct ccagcggggc gttgcatgcc cgccccacgt gccccatctt gtggcggcag
     6781 tagcagatgc acggtggtct gcccggtcaa cgcactgcag ttggtcgccg ccggtgagca
     6841 gggaaggctg g
//
LOCUS       DATALS010000125         5369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_145522, whole genome shotgun sequence.
ACCESSION   DATALS010000125 DATALS010000000
VERSION     DATALS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145522"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VHS06_02700"
     CDS             <1..622
                     /locus_tag="VHS06_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2987062.1"
                     /translation="GRGESGDSAEYSIEREFEDVAALVDSIPEPVMLLGHSYGGICAL
                     EASLRTQNIKKMVLYEPAVAVEGVKAHGDDVIEKLLSLQERGDREGILATFTQEVLRM
                     PAADFQRWRTDPTWQSRLAAAHTIPREIQARAQYRLDPKRFSSVKVPTLLLMGDRSPA
                     FLQAATRAVHAALPNSELVVMPNQGHVAMDSAPGLFAHEVLSFLTR"
     gene            complement(662..1420)
                     /locus_tag="VHS06_02705"
     CDS             complement(662..1420)
                     /locus_tag="VHS06_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX2987063.1"
                     /translation="MTDRAVGSARHHDSAVSIDGATEDRYLRLLSGAYLKQQAASVRI
                     LREHKGAGAIRHLARDAHSRAHRCAGAGLRITPPSEPLACGKGCYHCCWATVGVVAPD
                     AIYMADELRSRLPEDEFSALVERSARLARRLQRMDRREKLAARLPCSLLKDGACSSYE
                     HRPLVCRWACAPSLQACHDHLVHRTTSFLEMEGVRYQPTQEVWRGLRAGLWEVGLDAS
                     LLSLNGALAIALADPDIAHRYLAGEDVFADARLE"
     gene            complement(1522..2214)
                     /locus_tag="VHS06_02710"
     CDS             complement(1522..2214)
                     /locus_tag="VHS06_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2987064.1"
                     /translation="MAKILVVDDEPVMLSTVRRVLLDAEFLVDTATEGESALTMAREG
                     SYDLIILDVRLPDIDGLTVCQTLRQESSIPILMLTVLGDCNDKVGGLEAGADDYLTKP
                     FNSRELVARVIALLRRSAFSAEPTTQLQSGCLQTAGLEIDLVRRQVTRRGQYLRLTPK
                     EFDLLVFLATHPGRAFAQGKLLEEVWRFDNPSDTRTVQVHIRWLRQKLEDDPSLPRLI
                     ETVRGKGYRFVC"
     gene            complement(2274..2627)
                     /locus_tag="VHS06_02715"
     CDS             complement(2274..2627)
                     /locus_tag="VHS06_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987065.1"
                     /translation="MVDDDVLQQKVLTAMLEQLGYRVRVAASGEDALDLLREQSADLL
                     ILDMVIPGRIDAVETYRRALEIYPEERAIIISGFAEPGRIAEVQSLGAGSFLCKPVNR
                     EKLAVAVRGELDRIR"
     gene            complement(2638..4692)
                     /locus_tag="VHS06_02720"
     CDS             complement(2638..4692)
                     /locus_tag="VHS06_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2987066.1"
                     /translation="MERDHSFPLKPLSEGILETEGFTVNSIGQGERSTSPRRGPEAAL
                     RQRQDSHVLETVQQFANLFELGERGIWALDTVGRLTFLNSAMARMLGCTEDEAVGLPL
                     FWFLDETWRADLETHLQQRRRGVAETYDLKFRRRDGTELWAIVSATPAFDSDGHYVGV
                     AGLASDITQWRRAEALLKESETRFRRLSEGPQSCIFRCETYPTQKCSYISPTVVGIVG
                     YTPEEFYRDPCLLDRLVHPEDRTSLRALRAAPMPSWTVTLRVLHKDGHTVWVEQRNVA
                     VYDGSGRKTAYEGIIRDVTRIKLGARRLSDLERFQNEMLDTAAVWIHIRDADGNILFW
                     NRAAERVTGYSRQEVLGHPGIWEWLYSNQTDRERALSWTKQTVLKRRRLDGFESTIRR
                     KDGKNRRIIWYSSNLALEEGSAAIAIGNDITEHRQMEEQLLRAQRLETAGKIAAQVAH
                     DFNNLLGPFVTFPEMIKQRMPGDELIAEYCDTMVQAAERMADINTDLLVLGMRGSLEH
                     RVVDLNQLVAEALSHATNVPDTLVVDLKLADDLMYVSGAPSQLLRVLANLIVNAREAM
                     QDQGTLTIQTESVYLDRPLSGYNRVEVGQYVRVTVADTGSGIPPDIRDKVFDPFFTTK
                     HMHRRGSGLGLAVVKNIICDHKGYIDLESEIAKGTTFSLYLPICGKTPIQESEVDIRG
                     GY"
     gene            complement(4855..>5369)
                     /locus_tag="VHS06_02725"
     CDS             complement(4855..>5369)
                     /locus_tag="VHS06_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX2987067.1"
                     /translation="EELQAAVSRFKVGEQDEVVMKRRQADATQVGQISVAVQQMESAS
                     QEVVGLMDSVSAVVEESTAATEEMAASSQQVNGAIEKVAAVSEETSASAEEVSASTEE
                     MSSQVEEMVAQSEELSRMAEELQAAVSRFKVGEQAEVVMKRRQADWQRLPQTQQQPTR
                     QSDVKPLPIG"
BASE COUNT         1017 a         1747 c         1542 g         1063 t
ORIGIN      
        1 ggggagaggc gaaagcggcg attccgcgga gtactccatc gagcgtgaat tcgaggacgt
       61 ggccgcgctg gtcgactcca tccccgagcc agtaatgctg ctgggacact cctacggcgg
      121 gatctgcgcg cttgaagcct ccctgcgaac tcaaaacatc aagaagatgg tgctgtacga
      181 gcctgcggtg gcagtggagg gggtcaaggc gcacggggac gacgtcatcg agaagctgtt
      241 gtcgctgcag gagaggggcg accgcgaggg gatactggca accttcaccc aggaggtctt
      301 gcggatgccc gccgccgact tccaacgatg gcggaccgat cctacctggc agtccagact
      361 cgccgccgcg cacacgatcc ctcgggagat ccaggccagg gcgcagtacc gtctcgaccc
      421 gaagcgcttc agcagcgtga aggtgcccac tctgttgctg atgggggacc ggagcccggc
      481 cttcctgcaa gcggccacga gggcggttca cgcggccctg cccaacagcg agttggtggt
      541 aatgcccaat caggggcacg tggcgatgga tagcgcaccc ggcctcttcg cccacgaggt
      601 gctgagcttt ctcaccaggt agcctatatg gagtgcggcg cgcgccgcac tccgtttcag
      661 gctactcgag gcgcgcgtcc gcgaagacat cctccccggc caggtagcgg tgggcgatgt
      721 ccgggtcggc cagggcgatg gccaaggcgc cgttcaacga caggagggag gcatccaggc
      781 cgacctccca cagcccggca cgaagaccgc gccacacctc ctgggtcggc tggtatctga
      841 ccccctccat ctccaggaag ctcgtagtcc tgtggacaag gtggtcgtgg cacgcctgca
      901 ggctgggcgc gcaggcccag cgacacacca gcggacggtg ctcgtagctg gagcaggccc
      961 cgtccttcag cagtgagcag ggaagccgcg ctgccagctt ctcccggcgg tccatccgct
     1021 ggagacgccg cgccaatcgc gccgaccgct cgaccagggc agagaactca tcctccggca
     1081 gccgggaccg tagctcgtcg gccatgtaga tggcgtccgg ggcaaccacc cccaccgttg
     1141 cccagcaaca gtgatagcaa ccctttccac aggccaacgg ctctgaaggg ggcgttatgc
     1201 gcaggccggc acccgcgcac cggtgcgccc gagagtgggc gtcccgcgcc aggtggcgga
     1261 tggccccggc gcctttgtgc tcccgcaaga tccgcacgga ggcggcctgt tgcttgagat
     1321 aggcgccgga tagcagccgc aggtagcggt cttccgtggc accatcgata gaaaccgccg
     1381 agtcatggtg tcgggcagaa ccgactgccc tatccgtcac gaggtggccc cccgcccctt
     1441 caaactgcaa ccaattgcca tgtcgatctc cccgttcgat tgtcgcgatc cgagcttgca
     1501 cgccccgcgc caggaatcag tctagcatac gaaccgatag cccttccccc gaaccgtctc
     1561 gatcaatctg gggagggacg gatcgtcttc gagcttttgc cgcaaccacc ggatgtggac
     1621 ctggacggtg cgggtatctg aaggattgtc gaacctccac acttcttcca acaacttgcc
     1681 ttgagcgaag gcacgtccgg ggtgtgtcgc gaggaaaacc aggagatcga actccttggg
     1741 ggtgagacga agatactggc cccggcgagt gacctggcga cgaacgagat ctatttccaa
     1801 tccggctgtc tgtagacagc ccgactgtag ctgtgtcgtt ggttccgcgc tgaacgccga
     1861 acgccttagc agagcgatga cacgggcaac gagttccctc gagttgaagg gtttggtcaa
     1921 gtagtcatcg gccccggcct ccaatccccc gaccttgtcg ttgcagtccc caagaacagt
     1981 aagcataagg atggggatgc tgctctcctg ccgaagggtc tggcacaccg tcagaccgtc
     2041 aatgtcgggt aaccgcacgt ccaggataat caggtcatag gatccttccc gggccatcgt
     2101 gagggcgctt tctccctcag tcgccgtgtc tacgaggaat tccgcatcga ggagaacccg
     2161 acggacggtg ctcaacatca ccggctcatc gtctacaacc agtatcttgg ccattgtaga
     2221 atgaaaccct gcctatcttg ccaaataaaa tggcaaccac gtcaaagaaa ggatcagcga
     2281 attcgatcta gctcgccgcg cacggcaact gccagcttct ctcggttgac cggcttgcac
     2341 aggaagctgc ccgcgccaag agactgcacc tcggcaatcc gccccggttc agcgaagccc
     2401 gagatgatga tagccctttc ctccggatag atctccaggg ctctccgata ggtctccacc
     2461 gcgtcgattc gcccaggtat caccatatcc aggataagga ggtctgcaga ctgctctcga
     2521 agcaggtcca gtgcgtcctc tccgctcgcc gcaaccctca cccgatagcc aagctgttcc
     2581 agcatggccg tgagcacctt ctgctgaagc acatcgtcgt caaccacaag caggcgctca
     2641 gtaccccccc ctgatatcca cctcactttc ctggataggt gtcttgccgc atatcggcag
     2701 gtagagagag aaagtcgtgc ccttggcgat ttcactctcc aggtcaatgt accccttgtg
     2761 gtcacaaatg atgttcttga cgaccgcgag gccgagtccg gaaccgcgac gatgcatgtg
     2821 cttggtggtg aagaagggat cgaacacctt gtctctgatg tcgggcggta tgccactacc
     2881 ggtgtcggcc accgtcacgc gcacatattg gccgacctcc acacggttgt acccgctcaa
     2941 tggtcgatcg aggtagacgc tctcggtttg tatggtcaga gtgccctgat cctgcatggc
     3001 ctcgcgagcg ttgacgatca agttcgccag cactctcaac aactgcgatg gagcaccact
     3061 aacgtacatc aggtcgtcgg ccagtttcag atccacgacc agggtatccg gaacgttggt
     3121 ggcatggctg agcgcttccg cgaccaactg gttcaggtcg acaacccgat gttccaggct
     3181 gccacgcatg cccaggacca ggagatcggt attgatatcc gccattcgct cggctgcctg
     3241 aaccatcgta tcgcaatact ccgcgatgag ctcatctcct ggcattcgct gcttgatcat
     3301 ttcgggaaag gttacgaagg ggccgagcag gttgttgaaa tcgtgggcta cctgcgctgc
     3361 aatcttgccc gccgtctcca gcctttgcgc gcggaggagt tgctcttcca tctgccgatg
     3421 ctcggtgatg tcgtttccga tggcaatggc cgcactaccc tcttccagcg ctagattgct
     3481 ggaataccag atgatcctcc tgttctttcc gtccttcctg cgaattgtac tctcgaaacc
     3541 gtccagccgc cgccgcttca ggacggtttg cttggtccag gatagtgctc gctcgcgatc
     3601 ggtctggttc gagtagagcc attcccagat cccagggtgc cccagcacct cttgtcgaga
     3661 gtagccggtg accctctccg cggctcggtt ccaaaagagt atgttcccat cggcatcgcg
     3721 gatgtggatc caaacggccg cggtgtccaa catctcgttc tggaatcgtt ccagatccga
     3781 tagtctccgc gcacccagct taatccttgt gacgtcacgg atgatgccct cataagcggt
     3841 tttgcggccg gagccgtcat agaccgccac attgcgctgc tccacccata ccgtgtgccc
     3901 atctttgtgc agaaccctca aagtcactgt ccagctcggc atcggagcgg ctcgcagggc
     3961 acggaggctg gtgcgatcct cggggtgaac cagtctgtcg agcaagcatg gatcccggta
     4021 gaattcctcc ggtgtatagc ccacgatccc gaccaccgtt ggactgatgt agctgcactt
     4081 ctgagtaggg taggtttcgc agcggaaaat gcagctctgc gggccctcgg acaatcggcg
     4141 gaagcgagtc tcgctctcct tcagaagcgc ctcagcccgt ctccattggg tgatgtcgga
     4201 agccaagccc gctacaccga cgtagtggcc atcgctatcg aaggcgggag tagcggagac
     4261 gatggcccac agttcggtgc cgtcccggcg gcgaaacttg aggtcgtagg tctccgcgac
     4321 gccccgacgg cgctgctgga gatgtgtttc caggtcggct cgccacgttt cgtccaggaa
     4381 ccagaacagt ggcaggccca ccgcctcgtc ctcggtgcag ccaagcatcc gagccatggc
     4441 actgttgagg aaggtcagcc tgcccacagt atccagcgcc cagattcctc tctcacccag
     4501 ttcgaacaaa ttggcaaact gctgtacagt ctcaagtacg tgtgagtctt gccgctgcct
     4561 cagcgcggct tcaggccctc tcctcggtga ggtcgatctc tccccttgtc cgatactgtt
     4621 caccgtgaat ccttcagttt ccaggatgcc ctcggataga ggcttcaatg ggaagctatg
     4681 gtcgcgctcc atcctgctcg ttctagaaca atgcgacgct caggtcaacc acgcgtacca
     4741 cccggtcacc gcgatcgtcc ataccccttt tcgagtcgaa gagactctgg caaaagggga
     4801 tcggcgggaa aatcaccccg ccgatccccg tcacgtgaga ggcttgagga tcgtctaccc
     4861 tatcgggagc ggcttgacat cgctctgtct ggtcggttgc tgctgtgtct gaggcagccg
     4921 ttgccaatcg gcttgccgcc gcttcatcac cacttcggcc tgctccccca ccttgaaccg
     4981 gctcaccgca gcctgaagtt cctcggccat tcgtgagagt tcctccgact gggccaccat
     5041 ctcctccacc tgggagctca tctcctcggt ggaggcgctc acctcttccg ccgaggcgct
     5101 cgtctcctca ctcaccgctg ccaccttctc gattgcgccg ttgacctgct gggatgaggc
     5161 agccatctcc tcggtagccg ccgtcgactc ttctaccacc gcggagaccg aatccatcag
     5221 gcccactact tcctgagagg ccgactccat ctgctgcacg gcgacggata tctggcccac
     5281 ttgagtggca tcggcttgcc gccgcttcat caccacttcg tcctgctccc ccaccttgaa
     5341 ccggctcacc gcagcctgaa gctcctcgg
//
LOCUS       DATALS010000126         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_145596, whole genome shotgun sequence.
ACCESSION   DATALS010000126 DATALS010000000
VERSION     DATALS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145596"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..753)
                     /locus_tag="VHS06_02730"
     CDS             complement(22..753)
                     /locus_tag="VHS06_02730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2987068.1"
                     /translation="MSGSARFPDVVVIGGGIIGAACGYYLAREGVSVYLLERSSLASG
                     SSGACEGNLLAWDKELTRELPLGRRSFQLWRELVDELEVDFEYQPKGSVMVAEDEQSL
                     AAVEQKVRDLAAAGVPGRMLDPKQLHEEEPALAGDLVGGALFPEDGQVEPRYATAAMV
                     EGGRRHGMVVRQDCPVRHIALSPEGRVEAVETVSGRIPTAAVVVAAGVWSRDIVATAG
                     LDVPVPAGLLYTIIGMWMASETSLK"
     gene            complement(769..1881)
                     /locus_tag="VHS06_02735"
     CDS             complement(769..1881)
                     /locus_tag="VHS06_02735"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX2987069.1"
                     /translation="MARPLRGILSALATPFSEDGSKIDEGALRELVDGTIAAGIHGLI
                     PCGSIGEFPILSVEERKRIAEVVVDQARGRVPVVPHTGSCSTADAIDLSRHAESLGAD
                     AIMIVQPYYEPTSLDELFGYFKDVSDAIHIPMMVYNNPAGTGMNPPTDFLIRMAREIE
                     NVQYIKDSSGDISAVQELMFKYGNEVTIFNGWDTIAFPVLALGGKGAVWGAGNVAPRQ
                     CAELFNLVEVGKLEEARALWVEKLWPVNQFLVTEGYTASVKAGANLIGFHVGNPRLPY
                     RPPFAGEGGGAEGAAGERGSHRGAPPAQVRLALLECGGLTPQGGAGQRGGRGQALSKP
                     ARCAGQHCGVKPPHSKTVVPSQAAPLRKRTTDPVGI"
     gene            complement(2095..3150)
                     /locus_tag="VHS06_02740"
     CDS             complement(2095..3150)
                     /locus_tag="VHS06_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX2987070.1"
                     /translation="MRLSNMITAIDAHAAGEPGRVIVGGVMDVPGKTMFEKMVYLREN
                     HDDLRKRMLREPRGYPALCCNLVLPPTHPDADAGFVIMEQVEYPPMSGSNTICTATAL
                     LETGMIPMVEPVTEFTLEAPAGLVKIRAEVSNGKVRSVTFRNVPAFAAYLDAKVEVPK
                     LGTVTVDVAYGGMFYVITDAASVGLRLTPDEGKDIVRLGEMIKAATREQLPVVHPENP
                     GISGVTIAEFSAPATRQDAAMKNTVVVSSGEFDWDRPDTWTGILDRSPCGTGTCAKMA
                     TLHAKGKLGLNQDFPHEGILGTLFTGRLVEETRVGQYPAVVPTIAGQAWIYAITQYVL
                     DAEDPFKEGFTVGDIWA"
     gene            complement(3422..>4317)
                     /locus_tag="VHS06_02745"
     CDS             complement(3422..>4317)
                     /locus_tag="VHS06_02745"
                     /inference="COORDINATES: protein motif:HMM:NF037640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2987071.1"
                     /translation="LVESGATVVGVVEATTRAEWLRHGYQMWGHWDRVVDAYEYERAL
                     LMAGVPRYFGHVVLRAEGGESVERVTIAPVDRQWRPLPEGEKSFDADLLCIGYGFLSS
                     MELPALLGCRLSYNVAQEQYLPLYDLNMESSVPGVFVAGETTGVGGAEMALAEGEVAG
                     LAGARLLGHELSPQLHGALEQARDRRRHQAGFGRMLNDLFAMRPGIYELPMPDTLICR
                     CEEVSVQDILVAVDRGARSANALKGWTRVGMGPCQGRVCGNLAAHLIARETGRPLKEV
                     LDFSVRPPIKPVPLGALATLI"
BASE COUNT          729 a         1549 c         1315 g          724 t
ORIGIN      
        1 cagcctggac gagctgttcg gctacttcaa ggacgtctcc gacgccatcc acattccgat
       61 gatggtgtac aacaatccgg cgggcaccgg cacatccaga ccggccgtgg caacgatatc
      121 tcggctccag acccccgccg caaccaccac tgcggcggta gggatgcgac cggacacggt
      181 ctctaccgcc tcgaccctgc cctcggggct gagcgcaatg tgtctcacag ggcagtcctg
      241 ccgcaccacc ataccgtggc gacggccccc ctccaccatc gcggcggtgg cgtagcgcgg
      301 ctcgacctgc ccgtcctctg gaaacagcgc ccccccaacg aggtccccgg ccaatgccgg
      361 ctcctcctcg tggagttgct tcgggtccag catccggccg ggcacccccg ccgccgccag
      421 gtcccgcacc ttctgctcca ccgcggccag cgactgctcg tcctcggcaa ccatgacgct
      481 gcccttgggc tggtactcga agtcgacctc cagttcgtcc accaactccc gccaaagctg
      541 gaagctgcgc cggccgaggg gcaactcccg agtgagctcc ttgtcccacg ccagcaggtt
      601 gccctcgcag gcgccggagc tacccgaggc gagggaggat cgctccagca ggtagacgga
      661 gacgccctcc ctggccaggt agtagccgca tgcggcgccg attatgccgc cgccgatcac
      721 caccacgtcg ggaaacctgg ccgagccgct cacttaatca cccctccgtc aaattccaac
      781 aggatccgtc gtgcgcttcc ggagtggggc cgcttgcgac ggcaccaccg ttttggagtg
      841 cggcggcttg acgccgcaat gttgccctgc acaccttgcc ggtttggata acgcttgacc
      901 ccgtcctcca cgctgccccg ccccaccttg cggcgtcaag ccgccgcact ccaaaagggc
      961 tagtcgcacc tgggcgggag gtgcccctcg atggctcccg cgttcaccag cagcaccctc
     1021 agctcctcca ccttctccgg cgaaaggggg gcgatagggc aggcgagggt tgcccacgtg
     1081 gaagccgatc aggttcgcgc cggccttcac gctggcggtg tagccctcgg tgaccaggaa
     1141 ctggttcacc ggccaaagct tctccaccca gagggccctg gcctcttcca gcttgcccac
     1201 ctccaccagg ttgaacagct cggcgcactg ccgcggcgca acgttcccgg cgccccacac
     1261 cgcgcccttg ccgcccaaag ccaggacggg gaaggcgatg gtgtcccatc cgttgaagat
     1321 cgtcacctcg ttgccgtact tgaacatcag ctcttgcacg gccgagatgt cgccgctgga
     1381 gtccttgatg tactggacgt tctcgatctc gcgggccatc cggatcagga aatcggtagg
     1441 ggggttcatg cctgtgcccg ccggattgtt gtacaccatc atcggaatgt ggatggcgtc
     1501 ggagacgtcc ttgaagtagc cgaacagctc gtccaggctg gtgggctcgt agtagggctg
     1561 gacgatcatg atggcatcgg cacccaggct ctcggcgtgc ctggagaggt cgattgcatc
     1621 ggcggtgctg caggaaccgg tgtggggaac cacgggaacg cgcccccggg cttgatcgac
     1681 caccacctcg gcgatccgct tccgctcctc gacgctgagg atcggaaact cgccgatact
     1741 gccgcatgga atgaggccgt ggatgcccgc cgcgatggtg ccgtcgacca actcgcgaag
     1801 ggcgccctcg tcgatcttac tgccatcctc ggagaaaggg gtagccaggg cggagagaat
     1861 gccgcgaagg ggtcgagcca tcggtgacct cctcatacag aaggatgcta cccaacagtc
     1921 cggggccaga cgccccgctg ctcccccttc cgcagccctt cccccacgaa tggggaagga
     1981 atctcagcat cgccaccagc ggcaggcgca tcccaacgct gcctcctact gaggaaggga
     2041 gagctcgcga acacggacgg acgaaaggca gtcttcaacc aatacccgca aaaactacgc
     2101 ccagatgtcc cccaccgtga acccctcctt gaaggggtcc tcggcatcca gcacgtactg
     2161 ggttatggcg tagatccagg cctgcccggc aatggtggga accaccgcgg ggtactggcc
     2221 gacccgggtc tcctcaacca ggcggccggt gaacagcgtc cccaggatgc cctcgtgggg
     2281 gaagtcctgg ttcaacccca acttgccctt ggcgtgcagg gtcgccatct tggcgcaggt
     2341 gccggtcccg catggtgatc gatccaggat gccggtccag gtgtccggcc ggtcccagtc
     2401 gaactccccg ctggagacga ccaccgtgtt cttcatggcc gcgtcttggc gggtggccgg
     2461 cgccgagaac tccgcgatgg tcacgccgga aatccccggg ttctccgggt gaaccaccgg
     2521 aagctgctcg cgggtggcgg ccttgatcat ctcaccgagg cgcacgatgt ctttcccctc
     2581 gtcaggggtc aagcgaagcc cgaccgaggc cgcgtcggtg ataacgtaga acatcccacc
     2641 gtaggccacg tccaccgtca ccgtgccaag cttcgggacc tcaaccttgg cgtccaggta
     2701 ggctgcgaag gccgggacgt tgcggaaggt gacgctgcgc accttgccgt tggatacctc
     2761 ggcccggatc ttcaccagac ccgccggcgc ctccagggtg aactccgtca ccggctccac
     2821 catcgggatc atcccggtct ccagcaacgc ggtggcggtg cagatggtgt tgctgccgga
     2881 catgggcggg tactccacct gctccatgat gacgaagccg gcatccgcat cggggtgggt
     2941 gggcgggagc accaggttgc agcagagggc gggatagcct ctcggctccc tcagcatcct
     3001 ctttcgcagg tcgtcgtggt tctcccgcaa gtagaccatc ttctcgaaca tggtcttgcc
     3061 tgggacgtcc ataactccgc ccactatcac ccgtccgggc tcgccggccg catgggcatc
     3121 tatcgcggtg atcatgttag atagacgcat ctctgcctcc gttcagtgct gggtcggtat
     3181 tcgtgttgta acattggggc tcgcataatg tagcgtcggc gctcgcgacg cgagcgagcg
     3241 cagcgttcgg gcccgacgtg gtacccattg cagcggctgt cgggaatgta gcgtcggggc
     3301 tcgcaacgcg agcgagcgca gcgttgcgcc cgacatcgtg ctcgttgcca cgggtacccg
     3361 tcgcccacga ggggcgacgc tacatctcgc accccggccc ctgtcccccg gcacctggcc
     3421 cctagatcag cgtggccagc gccccgagcg gcaccggctt gatgggcggg cgaaccgaga
     3481 agtccagaac ctccttcaac gggcgtccgg tctccctcgc tatcaggtgc gcggccaggt
     3541 tgccgcagac gcgaccctgg caggggccca tccccaccct cgtccagccc ttcagcgcgt
     3601 tggcgctccg cgcgccacgg tcgacggcca ccagaatatc ctgaaccgat acctcttcac
     3661 agcgacagat gagggtgtcc ggcatcggca gctcgtagat gccgggtcgc atggcaaaga
     3721 ggtcgttcag catccggccg aaaccggcct ggtgcctgcg acggtcgcgc gcctgctcca
     3781 acgccccatg caactgcgga ctgagttcgt gtcccagcag ccgggcgccc gccaaccccg
     3841 cgacctcccc ctcggccagc gccatctcgg ccccaccgac ccccgtcgtc tcgcctgcca
     3901 cgaacacgcc cggcacgcta ctctccatgt tcaggtcgta gaggggcagg tactgctcct
     3961 gggcgacgtt gtagctcagc cggcagccca gcagtgcggg tagctccatg gaggagagaa
     4021 agccgtagcc gatgcagagc agatccgcgt cgaagctctt ttcgccttcg ggcagcggcc
     4081 tccactgccg gtctacgggc gctatggtca ccctttccac gctctcccca ccctcggcgc
     4141 gaagcacgac gtggccgaaa tagcgcggaa cgcccgccat cagcagcgcg cgttcgtact
     4201 cgtaggcgtc caccacccgg tcccagtgcc cccacatctg gtagccgtgg cgcagccact
     4261 cggctcgcgt cgtggcctcc accaccccca ccacggtggc ccccgactcc acgagca
//
LOCUS       DATALS010000127         4538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_191927, whole genome shotgun sequence.
ACCESSION   DATALS010000127 DATALS010000000
VERSION     DATALS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191927"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VHS06_02750"
     CDS             complement(<1..605)
                     /locus_tag="VHS06_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987072.1"
                     /translation="MINRKFVLCIGTAAGIVLLTLSTAGSVFAAPSSLLPYDMAPTPP
                     PMVLATSAPSAPQPTATPAPTKPAAQPTAAPAATAAPTAVLGASPSQAPAKAASQAKD
                     PWYFEQTGFKIANDRFWDYFNKRGGVGTFGYPISKEFLLFGFRVQLFQRALMQLNPDG
                     SVATMNLLEIPISPMKGENDTVNAYIVVHDKLDRKVETCES"
     gene            959..1507
                     /locus_tag="VHS06_02755"
     CDS             959..1507
                     /locus_tag="VHS06_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987073.1"
                     /translation="MRELDRRIAALRLRLAEINSRHEQLQTLQKQFQNQISSIVDFAA
                     LENRDLSSVLSMAEEVDTRLRQTEQTLRLLEAIRARGEEELETLLLTRTVDNTKAELA
                     ERANQLQHVRDEIARIEGTGIAQPIPDSGEESEMQRLRSRQAEIEAEIQRLHDRIDEA
                     SEAAARAVSDRGHRGIGEERGP"
     gene            complement(1556..1771)
                     /locus_tag="VHS06_02760"
                     /pseudo
     CDS             complement(1556..1771)
                     /locus_tag="VHS06_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            complement(1784..2236)
                     /locus_tag="VHS06_02765"
     CDS             complement(1784..2236)
                     /locus_tag="VHS06_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2987074.1"
                     /translation="MALISEKDREFLTDYFAKQLKDPVQITYFTQHDSQLAAPSQECM
                     YCKDTREILEEVAGLSDKIELKVYDFVADAQEAERLGVDRIPATVISGNAKGKVRFFG
                     IPSGYEFSSLVEVIADISRGETNLSDVTKAELAKVTEDVHIQVFVTPT"
     gene            complement(2345..3529)
                     /locus_tag="VHS06_02770"
     CDS             complement(2345..3529)
                     /locus_tag="VHS06_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2987075.1"
                     /translation="MDRKRARIGLIGAGFVSHIHARSYSRLTDLGIDLVAVAAVPRSQ
                     AEELARDFGIPNVYEDYRKVLERDDVDIVDICVPNFLHEPFVLEAAAAGKQIICEKPL
                     TGYFGGPDAADPVGATPRKIMLEGAVKSADRMLAAAEEHGVRLMYAENWLYSPSVQKA
                     SSLVAASGGTILEMRAQECHSGSHASYAKLWKLSGGGALVRLAPHPLGCCIYMKWQEG
                     LRRDGKPITVKSVTAEVADLSSIPSVQAEQEKWIVADWQDVENWVSVLLTFSDGSRAT
                     VFASDAVLGGMEDTLELFLSNSRIKCDMTHSNMMQAYAPSSKVFEHEYLMEKLETKAG
                     WSMPSIDEEWLLGYPQELRDFVESFIQDREPVSTARLGRDVVHVMYAAYQSAEEGRRI
                     GL"
     gene            complement(3536..4336)
                     /locus_tag="VHS06_02775"
     CDS             complement(3536..4336)
                     /locus_tag="VHS06_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEX2987076.1"
                     /translation="MSIEKAPTMVERIETGRLITEVYENRVDLGVAAAWAVDAEMRSL
                     LEKKQMIRMIFASAPSQNEFLATLRKLPGIDWDRVTAFHMDEYVGLDPDAPQSFSHFL
                     MESLFGAVEPAAFHKLNGMASDLKEECRRYQDLLKEGPVDIVCAGIGENGHLAFNDPP
                     VADFNDPKAVKVVDLTLASREQQVHDGCFPNLGVVPIQALTLTVPALMGAGSIYCMVP
                     GPTKAEAVRDTLLEPISTACPATIMRRHGAARLYADRESAALYMKARG"
BASE COUNT          958 a         1439 c         1271 g          870 t
ORIGIN      
        1 taactctcgc atgtctcaac ctttcggtct agcttgtcgt gtacaacaat atacgcgttg
       61 accgtgtcat tctctccctt catggggcta atgggaatct ccagcaggtt catagtggcc
      121 accgacccat cggggttcaa ctgcatcagg gcacgctgga acagctgtac cctgaatccg
      181 aacagcagga actccttgga gatgggatag ccgaaggtcc ccaccccacc acgtttgttg
      241 aagtagtccc agaagcggtc gttggcgatc ttgaaccctg tctgttcgaa gtaccaggga
      301 tctttggcct gggaggccgc ctttgccgga gcctgcgagg gactggcccc aagaacggcg
      361 gtaggagcag cagtggctgc cggtgcagcc gttggctggg cggcgggctt tgtgggcgca
      421 ggagtcgcag taggctgcgg cgcagacggt gcggaggtag ccagcaccat gggaggcggg
      481 gtcggtgcca tgtcataggg taacaaactg gaaggggcag cgaagaccga gcccgctgtc
      541 gacaatgtca gcaacactat tccagcagct gtgccgatgc acagcacgaa tttgcgatta
      601 atcacgatgt gaacaacccc gtctctgaag agtgaatggc agtggtggac ggccctccgg
      661 ttggacggca aacacacaag gggcaatgaa agaaccgccc tcgttggcga cgtggccgca
      721 ctaggcggcc ttccctagat gcagtcaacc tctccatgag gggaaagtgg ataccaacaa
      781 gggaaagtcg cttagagggc tggagccgag tgcacaaggt tcaggatatg cgatcaagag
      841 aaatagatca accttccaca cccaaatagg agattcccat tagccccatg aagggagaga
      901 atgacacggt caacgcgtat attgttgtac acgacaagct agaccgaaag gttgagacat
      961 gcgagagtta gacaggagga tcgcggcact gcggctccga ctggcagaga tcaactcgcg
     1021 ccacgagcag ctacagacac tgcagaagca gttccagaat cagatcagca gcattgtgga
     1081 cttcgccgcc ctcgagaaca gggacttgag ctccgtgctg tctatggccg aggaagttga
     1141 cactcggctg aggcagacag agcagactct gcggctgcta gaagcgatcc gagcccgcgg
     1201 cgaggaggaa ttggagactc tgctgctcac ccgcacagtc gacaacacca aggccgagct
     1261 agcagagcgg gcaaaccaac ttcaacacgt tagggacgag atagcccgaa tagaggggac
     1321 aggcatcgct caaccgattc ctgactcagg agaagagagt gagatgcagc gacttcgatc
     1381 gaggcaggcc gagatcgagg ccgagatcca gcgactccac gataggatcg atgaggccag
     1441 cgaggcggcg gcgcgtgccg tctctgacag gggccacagg ggaattgggg aggaaagggg
     1501 gccctgaccg tcaaagcgtc agcgcccctt tccccaactt tcgggccctt gcctgctacg
     1561 gagttccgcc tccaaccgcc ttcttcacct ctgccaggaa acgctcttcc ggcagcgccc
     1621 cgacgaactg ggtcttctcg ttcaccacta tcttggggac gctcatcacc cgatacttct
     1681 gggacatctg cgggaactcc gtcgcttcga ccgtgtccgc gatcacgtgc tcactctcga
     1741 tcgccatctt ctgggccatc ctggccgcat tgggacagta gggtcaagtg ggggtaacaa
     1801 acacctggat atggacgtcc tcggtgacct tcgccagttc cgctttcgta acgtcgctga
     1861 ggttggtctc tccccttgag atgtccgcaa tcacctccac gagggagctg aactcgtacc
     1921 cggatggtat cccaaagaag cggaccttcc ccttggcgtt gccgcttatt accgtggccg
     1981 gtatcctgtc cactcccaac cgctccgcct cctgcgcatc ggcgacgaag tcgtacacct
     2041 tcagctctat cttgtcagaa agcccggcca cctcctccag tatctcccgg gtatccttgc
     2101 agtacatgca ctcctggctt ggcgcggcca gctgggagtc gtgctgcgta aagtaggtga
     2161 tctggacagg atccttcaac tgcttcgcga aatagtccgt cagaaactct cgatccttct
     2221 ccgaaatcag cgccaccgat tcccccttcc cgcctgctct accacaggca catctctccc
     2281 gccccaacgg gcagcatctt ctacagcgct ggtgcacacc ccgtgcctag gcggaaacta
     2341 gtctctacaa accgatgcgc cgaccctcct ccgcggattg ataggctgcg tacatcacat
     2401 gcaccacgtc acggcctagt ctcgccgtgg acaccggctc cctatcctgg atgaatgact
     2461 ccacaaagtc gcgtagctcc tgagggtagc ccagcagcca ctcctcgtcg atgctaggca
     2521 tactccagcc cgctttcgtc tccagtttct ccatcaggta ctcgtgctca aagacctttg
     2581 aggatggtgc gtacgcctgc atcatattgc tgtgggtcat gtcacacttg atccggctgt
     2641 tggagaggaa cagttcaagg gtgtcctcca taccgcccaa caccgcgtcc gaggcaaaga
     2701 cggtggcgcg agagccatca ctgaatgtga gcagcaccga cacccaattc tcgacgtcct
     2761 gccagtcggc cactatccac ttctcctgct cggcctgcac cgacgggata ctactgaggt
     2821 ccgccacctc ggcagtcacg gacttcacgg tgataggctt gccgtcgcgt cgaagcccct
     2881 cctgccactt catatatatg cagcaaccca aagggtgagg agccagccgc acgagggcac
     2941 cgccaccgga cagtttccac agcttggcgt aacttgcgtg ggagccgctg tggcactctt
     3001 gtgcccgcat ctccagaatg gtaccgccgc tggcggccac taggctcgac gctttctgta
     3061 ccgaaggtga gtagagccag ttctcggcat acatcaaacg gacgccgtgc tcttcggcgg
     3121 cggccagcat cctatcagcg cttttcaccg ccccctccag catgatcttg cgcggcgtag
     3181 cacccactgg gtcggcagcg tcaggcccgc caaagtagcc ggtcaacggc ttctcgcaga
     3241 tgatctgctt gcctgccgcg gcggcctcca ggacaaaggg ctcatgcagg aagttgggca
     3301 cgcagatgtc gacgatgtcg acgtcgtccc tctccaacac cttacggtag tcctcgtaca
     3361 cgttgggaat gccgaagtcc cgagccaact cctcagcctg gctccgggga acggccgcta
     3421 ccgccaccag atcgataccc aagtcggtca atctggagta agagcgggcg tggatatggg
     3481 aaacgaaacc tgccccgatc aacccgatgc gagccctctt ccggtccatg cccccctacc
     3541 cccgagcctt catgtagagc gctgcagact ccctatctgc atacagccgc gccgcgccat
     3601 ggcgacgcat gatagtggcc gggcaggccg tagagattgg ttccagcagc gtgtcgcgga
     3661 ccgcctcggc cttggtcggg cctgggacca tgcaatagat gctgccggca cccatcaacg
     3721 ccggcacggt cagggtcagt gcctgaatcg gcaccacccc caggttgggg aagcagccgt
     3781 cgtggacctg ctgctccctg gaagccagag tcaggtctac caccttcacc gccttgggat
     3841 cgttgaaatc ggctaccggc gggtcgttaa aggccagatg cccgttctcg cctatgccgg
     3901 cgcaaacgat gtccaccggc ccttccttca gtagatcctg gtaacgacgg cactcctctt
     3961 tcaggtcgct cgccattccg ttcaacttgt ggaaagcggc cggctcgacc gcgccgaaca
     4021 ggctctccat cagaaagtgt gagaagctct ggggcgcgtc gggatcaagg ccaacatact
     4081 cgtccatgtg aaaggcggtg acacggtccc agtcgatgcc gggcagcttc ctcaacgtag
     4141 ccaggaactc gttctgagag ggagcagaag cgaagatcat cctgatcatc tgcttcttct
     4201 ccaggagact ccgcatctct gcatccaccg cccaggcagc cgcaacaccc aggtctaccc
     4261 gattctcata cacctcggtg atcaggcgac ctgtctcgat cctctcaacc atcgtcgggg
     4321 ccttttcaat cgacatgtca gtccccttcc tcacgacgcc catggagcgc cgatgatctc
     4381 aataccacct attcgccgag gcagggcccg ggtaccccca gacaccgtca tggctgatct
     4441 ccaacaaggg caggtagtcc agctactcct tgctcagggc aggcacggag tcctcgccac
     4501 tcactttcac aacgccggcc cctgagtgga gcagaaac
//
LOCUS       DATALS010000128         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_11870,
            whole genome shotgun sequence.
ACCESSION   DATALS010000128 DATALS010000000
VERSION     DATALS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11870"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VHS06_02780"
     CDS             complement(<1..242)
                     /locus_tag="VHS06_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987077.1"
                     /translation="MRRARSQPPQSLPSLRRVRNRYNGDVNGNFLCDRGRFGYEYVNS
                     ERRIREPLVRGGSTETTETEEAVTVRGAPQTAREGVP"
     gene            230..>2106
                     /locus_tag="VHS06_02785"
     CDS             230..>2106
                     /locus_tag="VHS06_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2987078.1"
                     /translation="MRGAPQTAREGVPPAIGPGGPNPVPIALDAVGRMLRGATGIIGI
                     GSPRASLESNYALRALVGPDSFCTGMSDVDHRLVATILEILRRGPVPSASLKDARESD
                     AVLVLGEDVTNTAPMLALSLRQSVRNRPMKRVAKLKIPEWHDDAVREAMGGEKGPFFL
                     ATVSETRLEEVATRSYHAAPEDIARLGFAVAGALQGERVSGEVDDLARAIAEALKGAE
                     RPLIVAGTSLGSDAVLHAAANVAFALQAAGSPVRLSFVVPECNSMGLGLMGGMSLGDA
                     FRAMGEGRADTVVVLENDLYRRAGATEVDAFFQTARNVVVLEQSANETTAGADIVLPA
                     AAYGEADGSYVSQEGRAQRFYQLFVPRGYMQESWRWIGEMAVAAGRAEVGSWDSLDAV
                     TTAMAAEIPALARVPEVAPSSEFRIAGQKIARQHTRFSGRTAMTANIDVSEPKPPDDP
                     DTPFAFSMEGYQGMPPSPLIPRIWAPGWNSEQALNKFQEEVPGPLKGGNPGIRLIEPT
                     AGGGIAPFKGMPEPFRARPGEWLVVPLFHIFGSEELSAMAPGIAELTPRAYLGLNPQE
                     AGRLGLAEGEEVDIELGGRPRRLVVKGIPTLPVGVAGVPVGFTGLEGIVLPAWARWAG
                     R"
BASE COUNT          332 a          651 c          779 g          344 t
ORIGIN      
        1 ggaacgccct cgcgtgcagt ctggggtgcg ccgcgcacgg tcacagcctc ctcagtctct
       61 gtcgtctctg tgctcccacc acgcacgagg ggttcccgta tccgccgctc gctgtttaca
      121 tactcgtagc cgaatcgccc ccggtcgcac aggaagttcc cgttcacgtc gccgttgtag
      181 cgatttcgga ccctgcgcag agacggcaga gactgagggg gctgtgaccg tgcgcggcgc
      241 accccagact gcacgcgagg gcgttccgcc tgccatcgga cccggcgggc cgaaccccgt
      301 accgatagcc ctggatgcag tgggacggat gttgcggggc gcgaccggga tcatcggcat
      361 cggctcgcca agggcttcgc tggagtccaa ctacgccctg cgcgccctgg tcgggccgga
      421 tagcttctgc accgggatgt ccgacgtcga tcatcgcctg gtcgccacca tcctcgagat
      481 cctccgtcga ggcccggtcc catcggcgtc gctgaaggac gctcgtgagt cggacgccgt
      541 gctggtgctg ggcgaggacg tcaccaacac tgcaccgatg ctggcgttgt cgctgcgaca
      601 gtcggtgcgc aaccggccga tgaagagggt ggcgaagctg aagatccccg agtggcacga
      661 cgacgccgtg cgtgaggcca tggggggcga aaaggggccg ttcttcctgg cgacggtgag
      721 cgaaacccgg ctcgaagagg tcgcgacccg cagctaccac gccgcgccgg aggatattgc
      781 tcgcttaggg tttgcggtag ccggggcgct ccagggcgag agagtgtccg gcgaggtgga
      841 cgatcttgcc cgcgccatcg cggaggcgtt gaagggggcc gagcggccgc tgatcgtcgc
      901 gggcacgagc ctcggcagcg acgcggtcct tcacgcggcg gccaacgttg cctttgcgct
      961 gcaggcggcg ggcagtccgg tccgcctctc cttcgtggtg cccgaatgca acagcatggg
     1021 gctggggcta atgggtggta tgagcctcgg cgacgccttc cgcgcgatgg gagagggaag
     1081 ggcggacacc gttgtggtgc tggagaacga cctgtaccgg agggctggcg ccacggaggt
     1141 ggatgccttc ttccagaccg cacgcaacgt cgtggtgctg gaacagtctg ccaacgagac
     1201 gacggctggg gccgatatcg tgctgcctgc ggcggcgtac ggtgaggcag acggcagcta
     1261 cgtgagccag gagggcaggg cccagcgctt ctaccagctc ttcgtcccgc gtggttacat
     1321 gcaggagagc tggcgatgga tcggcgagat ggctgtggct gccgggcgag ccgaggttgg
     1381 ttcctgggac agcctggatg ccgtcacgac cgccatggcc gcggaaatcc ctgcactggc
     1441 gagggtcccc gaggtggcgc catcctccga attcaggatc gctggacaga agatcgcgcg
     1501 gcagcacact cgcttcagcg ggcgcacggc gatgaccgcc aacatcgacg tgagcgagcc
     1561 aaagcccccc gacgacccgg acactccctt tgccttctcc atggaggggt atcagggtat
     1621 gccgccgtcg ccgctgatcc cgcgcatctg ggcgccgggc tggaactcgg agcaggcgct
     1681 gaacaagttc caggaggagg tccccggtcc gctcaagggc gggaatccgg gcataaggct
     1741 gatcgagccg accgcaggtg gcgggattgc gcccttcaag gggatgccgg agccgttccg
     1801 tgcccgaccc ggggagtggc tggtggtgcc gctgttccac atctttggat cggaggaact
     1861 gagcgccatg gcgccgggta tcgcggagct tacgcctcgc gcctacctgg gtctcaatcc
     1921 acaggaggcg gggcggctcg ggctggccga gggggaagag gtggacatag agctgggagg
     1981 caggcctcgc cggcttgtgg tgaaggggat accgacgctg ccggtgggcg tggccggggt
     2041 gccggtgggc tttacgggcc tggaggggat cgttctacct gcctgggcca ggtgggccgg
     2101 aaggta
//
LOCUS       DATALS010000129         4754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_11918,
            whole genome shotgun sequence.
ACCESSION   DATALS010000129 DATALS010000000
VERSION     DATALS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11918"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHS06_02790"
     CDS             <1..235
                     /locus_tag="VHS06_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987079.1"
                     /translation="AYPEGLDPTFARVAVAEMERLLSDDSVGTSMDSTLFRQLYENHP
                     CRSCSYHEDKHWQICAECHFAGYPINFERPSRQ"
     gene            399..1502
                     /locus_tag="VHS06_02795"
     CDS             399..1502
                     /locus_tag="VHS06_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2987080.1"
                     /translation="MSGEEEAPRPRIEAPNTAATEREDSKTRVKREPIWITLLVLAII
                     AIFLIVVREVLTPFIVAGVLAYIAAPAVKWVQKRTRLPRPAAVFGFFLIFIVPIGVLI
                     YLLEPALVSETKELATNTPAILGNLLYQIFGGNSVELLGQTFDAGTVSDYLLHATRSV
                     LGTPSEAIHVASSILGALLHAFLSIVLLFYFLLSPISVGQLVLRLIPAEYRPHWRRVG
                     REVHEVMGHYVRGLVFLVFLMAAVTWVGLTAIFHLPFALPIALATGFLEIIPFVGPVI
                     AASIAAVVGLFYGGAGYAASIALFYFIIREIEDQLVMPNVIGRAVEIPPALAIFAVLA
                     GQAIAGVLGALLGIPVAAAVKIGFDRWRPPQEL"
     gene            complement(1524..2375)
                     /gene="lgt"
                     /locus_tag="VHS06_02800"
     CDS             complement(1524..2375)
                     /gene="lgt"
                     /locus_tag="VHS06_02800"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX2987081.1"
                     /translation="MPGFIVIDINPILAQLGPIAVRWYGLMYDVGVVAGLLVAYPFAR
                     QKGLTDDDIWALLWPSIIAGFVGARLYYVVQQPLGPYLAEPWRIVATWEGGMAFYGAI
                     FGVIIALAIVARRRKLSLWTIFDAAVLLAVVGQAFGRIGNIVNGDIVGAPTTLPWGFI
                     YTHPNSFVADHTVAYQPAAVYELIFNLLFFAVLWYLRFKLPKAGLLFVVYLVGYSVGQ
                     FALFFLRTEPLVAAGLKQAQLTALVVLLAALAVGWWLMYREERMPVQVMPSAPKGPAR
                     LQPKRRR"
     gene            2548..3315
                     /locus_tag="VHS06_02805"
     CDS             2548..3315
                     /locus_tag="VHS06_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX2987082.1"
                     /translation="MHIPDGVLPIWLIAAGWLLTVALLALASRSLQERVIGARLPLLG
                     VMSALMLVAMSLELAPIGYHINLTVISGIVLGPALGFLAAFLVNLVLAFFGHGGITVV
                     GLNTLIIGVEIVLGYYLFHGIRAIFGLRPGVIVEAGMTAVLALVCSTFLMFGIVALSN
                     VNPGQVTHEEGAPRPSTLTFRNPVGQGALSIEPFPHEEQPGPAQGIDLFTFMRLVLGL
                     GSVGWILEALITGLIAGYVARVRPDLMGWPSELPGGG"
     gene            3321..4133
                     /locus_tag="VHS06_02810"
     CDS             3321..4133
                     /locus_tag="VHS06_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX2987083.1"
                     /translation="MDISTLDLWATSGRSPLHRASALSKVVAALLVIAAIVAAQDLFV
                     LLAIYLVLVAGVLTSRLPTLRVLSLAAYPALFALLFAVSAWNGSLLDPLLIITRAVTA
                     ATTMLLLIVTTPYHQLFAVLRHLLPKSIADALFITYRSIFILLDILGALLTALSLRGG
                     FLRGKPLHNIRSLAAGVGLLLLRAIAYSEQLYTIMQVRGYSGTMVGGASWRQTTRMDL
                     VPLLTAAAILAYVLLTRMLPEALQFNGDLLVLTFLAATAAAATRILYSRPSW"
     gene            4138..>4754
                     /locus_tag="VHS06_02815"
     CDS             4138..>4754
                     /locus_tag="VHS06_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2987084.1"
                     /translation="MSRIVTATNVHHIYPDGTELHYDGIPFTADRGERIVLLGPNGSG
                     KTTLLYHLLGLLKSSHGIVRVFGVDPARHFDRIRERIGVVLQDPDRQIIAPTVKDDIA
                     FSPRNYGLPEDEVGRRVHEVAAQFEVEPLMDKVPQYLSGGEKTKVALAGALVMRPHLL
                     VLDEPFEGLDTVAKSELAALLSRLHAERGISIIATTHEINLIPEFV"
BASE COUNT          888 a         1516 c         1410 g          940 t
ORIGIN      
        1 cgcctaccca gaggggttgg accccacctt cgcccgtgtc gcggtggcgg agatggaacg
       61 tctcctgagt gacgactcgg tcggaacatc catggatagc accctcttcc gccaactgta
      121 cgagaaccac ccctgccgca gttgcagcta ccacgaggac aaacactggc agatctgcgc
      181 cgagtgtcat ttcgccggct atccgataaa cttcgagaga ccgagcagac aataggacgc
      241 cgggggctat tcggtctctc gccctctccc tgtaccggca ggccgacaac ggcgccactt
      301 gccggccgcg ctctcccctt cgggcaacat gaacggaatg tcagagaacg cgatgtcctg
      361 gcaccgagag tggtattatc ccgggtggag ggacgacatt gtcaggcgaa gaggaagccc
      421 ccaggccgcg catcgaggca ccaaatactg cggcgacgga gcgagaggac tcgaaaacgc
      481 gcgtcaagcg agagcccatc tggatcactc tcctcgtgct ggccataatc gccatcttcc
      541 tcatcgtcgt gagagaggtc ctgaccccct tcatcgtcgc cggggtcctc gcctacatcg
      601 cggctcccgc agtcaaatgg gttcagaaac gtactcgact gccacgacct gccgcagtat
      661 tcggcttctt tttgatcttc attgtcccca taggggtgtt gatctatctc ctggagccgg
      721 cgctcgttag cgagaccaag gaactcgcta ccaatacccc agcgatactg ggcaacctgc
      781 tctaccagat cttcgggggg aacagcgtag agctgctggg tcaaaccttc gacgcgggga
      841 cggtcagcga ttacctgttg cacgctaccc ggagtgtgct cggcaccccc tcggaagcga
      901 tccacgtcgc atcgtccatc ctgggagcgc ttctccacgc cttcttgtcc attgtgttgc
      961 tcttctactt tctgctgagc cccatctccg tcggccagct ggtccttcgg ctgatcccgg
     1021 cggagtatcg cccacattgg agaagggttg gacgagaggt tcacgaggta atgggacact
     1081 acgtccgcgg cctggtcttc ctggtattcc tgatggccgc cgtaacctgg gtcgggctaa
     1141 cggctatctt tcacctgcca ttcgcgctgc ccatcgctct ggctacgggc ttcctggaga
     1201 tcattccgtt cgtcggtccg gtgatcgcgg cgagcatagc cgcggtcgtc gggctcttct
     1261 atgggggtgc cggatatgcg gcgagcatcg cgctcttcta cttcataatc agggagatcg
     1321 aggaccagtt ggtcatgcct aatgtcatag gcagagccgt ggagatccca ccggcattgg
     1381 cgatattcgc ggtacttgcc ggacaggcca ttgcgggagt actgggggcg ctcctcggca
     1441 taccggtggc ggccgccgtg aagatcgggt tcgatagatg gagaccgccc caggagttgt
     1501 gacggggcgg tccccaatct ctgtcaccgg cgccgcttcg gctgcaacct cgcgggtccc
     1561 ttcggggctg aaggcatcac ctgaacgggc atgcgctcct ccctgtacat gagccaccat
     1621 cccaccgcca gggcggcgag cagtaccacc agcgcggtca actgcgcctg cttgaggccg
     1681 gccgcgacca gcggctcggt gcgcaggaag aacagcgcga actgcccaac cgagtagcca
     1741 accaggtaca ctacgaagag gaggcccgcc ttgggcagct tgaacctgag gtaccagagc
     1801 accgcgaaga acagcaggtt gaagatcaac tcgtatacgg ccgcaggctg gtaagccacc
     1861 gtgtggtccg caacaaagga gttgggatgg gtgtagatga agccccacgg gagcgttgtc
     1921 ggagcgccca cgatatcgcc gttcacgatg ttgccgatac gcccaaaggc ctgccccacc
     1981 acggccagaa gcacagctgc gtcgaaaatc gtccagaggg atagcttgcg tcgccgggcg
     2041 acgatggcca gcgcgataat cacaccgaag atggcaccgt agaaggccat tccaccctcc
     2101 caggttgcca cgatccgcca cggttcagcc aggtaggggc cgaggggctg ctggaccaca
     2161 taatagagcc gcgccccgac gaagccggcg ataatggagg gccacagtaa cgcccagatg
     2221 tcgtcgtcgg tgagcccctt ctgtcgagcg aaagggtagg ctaccaacag ccctgccacc
     2281 actcccacat cgtacatgag gccataccag cgcacggcga ttgggcccaa ctgcgccagg
     2341 attggattga tgtcgatgac tatgaaaccc ggcattgcgc tgttactcct gtttctatcg
     2401 cgctaaactg cgtggaatta tagaggcaaa gccgcacccc gtccacctct ggaggcacat
     2461 atggcatatc tcctgccgag acgcctggcg cgaaacctgc ctcagtagtc cccacagctc
     2521 aagcgacggg gtgggcgcgt gtcccatttg cacatacccg atggggttct gccaatctgg
     2581 ctgatcgcag cagggtggct gctcacggtg gcgctgctgg cgttggcgtc caggtctctc
     2641 caggaaaggg ttatcggggc gaggttgcca ctcctggggg ttatgtcggc actgatgctg
     2701 gtggctatgt cgctggaact tgcgcccatc ggctaccata tcaacctcac cgttatcagt
     2761 ggcattgtgc tggggcctgc actaggcttc ctggcggcct tcctggtgaa cctcgtcctg
     2821 gccttcttcg gacacggtgg catcactgtg gttgggttga acaccctgat catcggtgtg
     2881 gagatcgtgc tgggctacta cctgtttcac gggatccggg ccatcttcgg ccttcggccc
     2941 ggggtaatcg tggaagccgg catgacagcc gtcctcgccc tcgtctgcag cacctttctc
     3001 atgttcggca tcgtcgcgct ttccaacgtg aaccccggtc aggtaacgca tgaagagggc
     3061 gctccgcggc ctagcaccct gaccttccgc aaccccgtgg ggcagggggc gctgagcatc
     3121 gagccgttcc ctcacgagga acagcccggt ccggctcagg gaatcgacct attcaccttc
     3181 atgaggctcg tcctgggcct gggctctgtg ggttggatcc tggaggcact gatcacgggg
     3241 ttgatcgcag gctacgtcgc acgggtgcgt cccgatctca tgggatggcc ctctgaactc
     3301 ccaggaggcg gataagcagc atggacatct ccaccctgga cctctgggcc acatcgggaa
     3361 gaagtccgct ccatcgcgca tccgcactgt cgaaggtggt cgccgcgctg ctggtgatcg
     3421 cggcgatagt cgcagcgcag gacctattcg tcctgctcgc catatacctc gtcctggtgg
     3481 caggagtcct gacgagtcgc ctacccacac tgcgcgtact ctccctggct gcctaccctg
     3541 cgcttttcgc gctgctcttc gcggtaagcg catggaacgg gtcgctgctc gacccgctgc
     3601 tgatcatcac gagggcggtc acggcagcaa ccacgatgct gctgctgatc gtcacgaccc
     3661 cgtaccatca actgtttgca gtactcaggc acctcctgcc aaagagtatc gcagacgccc
     3721 tgttcataac ctacaggtcg atcttcatcc tgttggacat cctgggcgca ctgctcaccg
     3781 ccctcagcct gcgaggcgga tttctcaggg gaaagccgct acacaacatc cgcagcctcg
     3841 ccgcgggagt cgggctactg ctgctcaggg caatcgccta ctcggaacag ctgtatacca
     3901 tcatgcaggt caggggctat tccggcacga tggtcggtgg agcctcctgg cgccagacaa
     3961 ccaggatgga tctcgttcca cttctgacgg ccgcagccat actggcctac gtcctgttga
     4021 ctcggatgct accggaggcg ctccagttca acggcgacct gctcgttctg acgtttcttg
     4081 ccgccactgc agccgccgcc acccgcatcc tgtactcgcg cccctcctgg tgacctcatg
     4141 tcccgcattg tcactgccac caacgtgcac cacatctacc ctgacggcac agagctgcac
     4201 tacgacggca tccccttcac cgccgaccgc ggcgagcgga tagtcctgct ggggccgaac
     4261 ggcagcggca aaaccacgct gctgtaccac ctgctggggc tgttgaagtc gagccacggc
     4321 atagtgaggg tgtttggagt agatcctgct cgtcatttcg accggattcg tgaaaggatc
     4381 ggggtcgtgc tacaggaccc cgaccggcaa atcatcgctc ccaccgtgaa ggacgacatc
     4441 gcgttctcgc cccgcaacta tggtctgccg gaggatgagg ttggccgcag ggttcatgag
     4501 gttgccgcgc agttcgaggt ggagccactg atggataagg tgcctcagta cctctcgggc
     4561 ggcgaaaaga cgaaggtcgc cctggccggt gcgctggtca tgcgtccgca tctccttgtc
     4621 ttggacgagc ccttcgaggg gctggacacg gtagcaaaat cggaattggc agcgcttctc
     4681 tcccgtctac atgcagaacg gggcatctcc atcatagcca ccacgcacga gatcaacttg
     4741 atccctgagt tcgt
//
LOCUS       DATALS010000130         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_146294, whole genome shotgun sequence.
ACCESSION   DATALS010000130 DATALS010000000
VERSION     DATALS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146294"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1002)
                     /locus_tag="VHS06_02820"
     CDS             complement(295..1002)
                     /locus_tag="VHS06_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2987085.1"
                     /translation="MRYYLGLAVAIVAISFSAIFIRLADAPALTIATGRMIVTVLMLL
                     PPLLLFERRSLAKLDAHDFALSLLSGLFLALHFALWTVSLDYTSVASSVVVVSTHPIF
                     VALIAYFYLGERLTAGGLAGIILTLGGSVLIGFKDLGVGQESLKGDLLALGGAIAIVG
                     YLLIGRVVRQRQGLLVYSTLVYTACAVALTAIALAVGTPLLAFSDRDLLVFLALAVHP
                     LSPKDFLEGSQESWGHP"
     gene            complement(1084..1785)
                     /locus_tag="VHS06_02825"
     CDS             complement(1084..1785)
                     /locus_tag="VHS06_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX2987086.1"
                     /translation="MNWEKILKLKEFDTPTVANGLELLKVMDPSAGYTGPDVRSLMPQ
                     LGRVVGIAATSRMDTTSPGMDNPPSLFNDWLRLMQKTASVEGGLTIPVFAVMESVGPR
                     PKYTVTIGDRMATLMKLAGAAGFVTNGSIRDLEGVTQVPLPSWGAGLSPMHGKLRWLD
                     VGSPVVIDGMTVRTGDIIMADENGVICIPPAVADQVYDKAVSVQQTEEGFFRKVSEPG
                     MTLDAYLGAYVKSSR"
     gene            complement(1880..>2461)
                     /locus_tag="VHS06_02830"
     CDS             complement(1880..>2461)
                     /locus_tag="VHS06_02830"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2987087.1"
                     /translation="EELAEQLRVSRMPVREAIQRLQVEGLVDMIPHRGATVAMATREQ
                     LGQVFAVRSVIEGLSAREAASRITGEEIDRLESLYREMEGVVANSDTDGQLCKNREMH
                     SILWEITGNPLLQSIARNLFDASERYRRQLMLQPRIAKEALEEHRAILDAISDRDPDR
                     AERLTREHIERTGRLMASYTRPISDGGNELDSQ"
BASE COUNT          541 a          788 c          674 g          458 t
ORIGIN      
        1 gatagggttg ctgaccgcgg caggagggtt ggccatgttg ctggggatct acctcacagc
       61 caggaattga gccccgtctc cttacggata ccccgtgtcc cctggcagaa ccgttcgtgc
      121 accacctcga ttagcacggg ctgttctgcc gaaaagagct ctctccgggg cgctgatttc
      181 ctgctagaat gcgtccgaac tgacactggt cggtccatct ggcaaatatc tgtcactacc
      241 ggcgggtcca gggcgccgcg cttgaagtgg ggtgaacgcg agcgatcgca gcgttcacgg
      301 gtgtccccaa gattcctgag atccctcaag aaagtccttt ggactcagtg gatggaccgc
      361 tagagctagg aagaccagca gatcccgatc ggagaaggca agcaggggtg tcccaacggc
      421 aagagctatc gccgtgaggg caacagcaca cgcggtgtac acaagggtgg agtagacgag
      481 cagcccctgc cgctgcctca ccaccctgcc gatgagcagg tacccgacga tggcaatggc
      541 accgccaagc gccagcaggt ctcccttcag actctcctgc cctaccccca ggtctttgaa
      601 gccgatcaga acgctgccac ccagcgtcag gataatccca gccagccctc ctgcagtaag
      661 cctctcgcca agatagaaat aggcgattag cgccacgaaa atggggtgag tgctcaccac
      721 caccaccgaa ctcgcaaccg acgtgtaatc cagagagacg gtccacagcg cgaaatggag
      781 agccagaaac agtcccgaga gtagcgagag ggcaaaatcg tgagcatcga gtttggccag
      841 ggagcggcgt tcgaagagga gtagcggtgg cagcagcatc aggacggtga cgatcatgcg
      901 gccggtggcg atggtcagag caggtgcgtc agcaagccgg atgaagatgg ccgaaaagga
      961 gatggcaact atggcgacag ccagacccag gtagtagcgc acaatagacc ccagactgaa
     1021 agtggaaagg ggcgggaacc ctcacgaaca ggccacatgg cccccgccct attagaagac
     1081 gatctacctg ctgctcttta cgtatgcgcc caggtaggca tccagggtca tgcccggctc
     1141 gctgaccttt cggaagaaac cttcctccgt ctgctgcacg gagaccgcct tgtcgtacac
     1201 ctgatcggcg acagccggtg ggatgcagat aacgccgttc tcatcagcca taatgatatc
     1261 gccagtccgc accgtcatcc catcgatcac gaccgggctt cccacatcca accatcgcag
     1321 tttgccgtgc ataggagata gtccagcgcc ccagctgggc agcggaacct gggtcacgcc
     1381 ttccaaatcg cgaatactgc cattggtgac gaaaccggca gcgccggcaa gcttcatcag
     1441 ggtggccatc cggtcaccga tggtcacggt gtactttggc ctggggccca ccgactccat
     1501 cactgcaaag acagggatgg tgaggccacc ctccacactc gcggtcttct gcatcaagcg
     1561 aagccaatcg ttaaagaggc tcggcggatt atccatgcct ggcgaggtag tatccatgcg
     1621 ggaagtcgcg gctatcccga ccaccctccc aagctgaggc atcagggagc ggacgtcggg
     1681 cccggtgtag cccgccgagg gatccatcac cttcaacagt tccagaccgt tcgctacagt
     1741 cggggtatcg aactccttga gcttgaggat cttctcccaa ttcatcactc actcctcatc
     1801 gaacgcagtg tgagaaatct cctatcgacc ccaccaaatg cgcaggcttt cgcgcgcaca
     1861 gtatctcact tatagcccct cattgagagt ccagttcgtt ccctccatcg gagataggcc
     1921 gcgtatagct cgccatcaga cgccccgttc gctcgatatg ctcgcgggtc aaccgctcgg
     1981 ccctatccgg gtctcgatcc gatatggcat ccaggatcgc gcgatgttcc tccaacgctt
     2041 ccttcgctat tcgaggctgc agcatcaact ggcgacgata ccgttcactc gcgtcgaaga
     2101 gattcctcgc aattgactgc agcagagggt tgccagtgat ctcccagaga atggagtgca
     2161 tctcacgatt cttacagagc tgtccatcgg tgtcgctgtt ggcaaccacg ccctccatct
     2221 ctcggtacaa cgattccagc ctatcgattt cctcgcccgt gattcggctc gcggcttccc
     2281 ttgcagacaa cccctcgatg accgatctca ccgcgaacac ctgccccaac tgctcacggg
     2341 tcgccatcgc cacagtagca cccctgtgag ggatcatgtc gaccaatccc tcgacctgga
     2401 gccgctgaat cgcctccctc accggcatcc ggctgacacg cagctgctcc gccaactcct
     2461 c
//
LOCUS       DATALS010000131         4564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_95930,
            whole genome shotgun sequence.
ACCESSION   DATALS010000131 DATALS010000000
VERSION     DATALS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95930"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VHS06_02835"
     CDS             <1..695
                     /locus_tag="VHS06_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019687334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2987088.1"
                     /translation="PEHIKEVAEASLGRLKVDAIDLFYQHRVDPDVPIEDVAGAVKDL
                     IREGKVKHFGLSEAGVQTIRRAHAVQPLTAVQSEYSLWWRRPEEELLPALEELGIGFV
                     PFSPLGKGFLTGKIDEKTTFDSSDFRNIVPRFTPEARKANQALVDLLHKVAERNKATP
                     AQIALAWLLAQKPWIVPIPGTRKLERLEENIGAVAVELTADDLREIDGAASKITIQGA
                     RYPEALEKRTGL"
     gene            700..1749
                     /locus_tag="VHS06_02840"
     CDS             700..1749
                     /locus_tag="VHS06_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX2987089.1"
                     /translation="MSRTIDRRRFLRNLLLLGAGYGILPNVACSPSGQAGPTPAVRLV
                     PTAGPTSTALSSPASVPTAAAVATTGHTVASTVAAGTSQLVVARGSSPEAITRSAIET
                     LGGMSRFVKPGAEVIVKPNICAANWSFEYAVTTNPEVVGTIVKLCVEAGAKRVRVMDF
                     PFSGTAEQAYSTSGIGDAVRAAGGQMEVMSSLRFGDVAIPNGRDLKTWKIYRDAVDPN
                     VVLINVPIAKQSSVVGVTMGMKNLMGLIQDRPSLHTNIDQRLADLNTAIRPALTVVDA
                     VRILLHDGPTGGSLDVVKKLDTVVASGDPVAADSYGATLFGRKGSDLAYIRNGAAMGL
                     GVMDLSQVAIKEVTA"
     gene            2246..3400
                     /locus_tag="VHS06_02845"
     CDS             2246..3400
                     /locus_tag="VHS06_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2987090.1"
                     /translation="MIYKEFKGITISHLGMGNMRLPTTGPGGLRAPIDRGKAQEIIDY
                     VYANGVNYFDTAYMYHGGESERFLGEALKKYPRESYLLADKMWGMLVQQGRPTADVFE
                     EQLRRCQTDYFDFYLLHNVSESTIDIYMDEKLGILDDLLEQKRTGRIRNLGLSSHGKP
                     ATLKRFIERWHCFDFVQIQLNYLDWTLQDAKGQYEVITEHGMPVWVMEPCRGGRLASL
                     SPEADEVLKKANPNASIASWAFRYLQTLPNVQVVLSGMTQMEQAVDNIRTFAHPNPLT
                     EEEQKALEQAVRLLKERLNVPCTKCRYCDGCPKGLDIPELLAMYNELCIDRGGALMIA
                     LGNMQGEKKPANCVACGQCAKKCPQNIDIPGIMSKFAETLAKMPPTALPR"
     gene            3354..3761
                     /locus_tag="VHS06_02850"
     CDS             3354..3761
                     /locus_tag="VHS06_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2987091.1"
                     /translation="MRRHWPRCLPRRYQDRVPWRTVVNIASVEAHTILREFPAYVASK
                     HGLIGLTKGTAYDYAAHGIRINSVSPGVIRTPLTMAEGQKEVTDRLAVRIPQGRLGEP
                     EEIARSVVFLLSDLSSYTTGADLVVDGGFLLRV"
     gene            complement(3749..4288)
                     /locus_tag="VHS06_02855"
                     /pseudo
     CDS             complement(3749..4288)
                     /locus_tag="VHS06_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011170751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(4442..>4564)
                     /locus_tag="VHS06_02860"
     CDS             complement(4442..>4564)
                     /locus_tag="VHS06_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX2987092.1"
                     /translation="AADIEVIPIDKVNEAYDRAVAKDVRFRFVIDNSTLPKVGA"
BASE COUNT          943 a         1307 c         1432 g          882 t
ORIGIN      
        1 ggccagagca catcaaggag gtcgccgaag cctcgctggg gcgactcaag gtcgatgcca
       61 tcgatctgtt ctatcaacac cgcgttgacc cggacgtccc aatcgaggac gtggcgggcg
      121 cggtgaagga cttgatccgg gaaggtaagg tgaagcactt cggtctttcc gaagcgggag
      181 tgcaaacgat ccgtcgcgcg cacgcagtcc agcccttgac tgcggtccag agcgaatact
      241 cgctgtggtg gagacgtcct gaagaggaac tgctgccggc gcttgaggaa ctcggaatcg
      301 gcttcgttcc atttagtcct ctgggcaaag gcttcctcac ggggaagata gacgagaaga
      361 cgacgttcga cagttccgac ttccgcaaca tcgtccctcg ctttacgccg gaggctcgca
      421 aggcgaatca ggccctggtt gatctgcttc acaaggtcgc agaacggaac aaggcgacac
      481 ctgctcagat cgcacttgcg tggctgcttg cccagaagcc gtggattgtc ccgatcccag
      541 gcacccggaa gctggagcgt ctggaggaga acatcggagc cgtcgcagtc gaactcaccg
      601 cggacgatct ccgtgagatc gatggcgccg cctcaaagat cacgatccag ggtgcgcggt
      661 accccgaagc cctggagaag aggaccggac tctgagaaga tgagccgaac catcgatcgt
      721 cgcaggttcc tgcggaacct cctgcttctg ggagcgggct acggcatctt gccgaacgtc
      781 gcctgctccc catcgggaca ggccggtcct acacccgccg tgcgcttagt gcccacggcg
      841 ggcccaacat ccacggcgct ttcgtcgccc gccagtgtcc ccactgccgc ggcggtcgcc
      901 acaactgggc acactgtcgc gtcaactgtc gcggctggca cctcccagct tgtagtggcg
      961 cgcggttcca gcccggaggc gatcacccga tccgccatag agaccctcgg cggtatgagt
     1021 cggttcgtga agcccggggc ggaggtcata gtcaagccga acatctgcgc tgccaactgg
     1081 tccttcgaat atgcggtcac gacgaaccct gaggtcgtgg gcaccatcgt gaagctctgc
     1141 gtggaggccg gggcgaaaag ggtgcgagtg atggactttc ccttcagcgg cacggccgaa
     1201 caggcgtaca gcactagtgg gatcggggat gccgttcgcg cggcaggtgg gcagatggag
     1261 gttatgtcca gcctcaggtt cggcgacgta gccatcccga atgggcgcga cctcaagacc
     1321 tggaagatct atcgggatgc ggtggacccg aacgtcgtcc tgatcaacgt gccgatcgcc
     1381 aagcagagca gcgtagtcgg cgtcaccatg ggtatgaaga acctgatggg cctgatccaa
     1441 gaccgaccgt ctctccacac caatattgac cagagactgg cggatctaaa caccgcgatt
     1501 cgccccgccc tcaccgtggt ggacgccgtg cggatcttgc tccacgatgg ccccaccggc
     1561 gggagcctcg atgttgtgaa gaagctcgat acggtggtcg ccagcggcga cccggtcgcg
     1621 gccgactcct atggcgcgac tctctttgga aggaaaggtt cggacctcgc ctatatccgc
     1681 aatggcgcag ccatgggcct gggagtgatg gacctttcgc aggtggccat caaggaagtg
     1741 accgcctgac tcgttgagcc atctgggcgc gctgctctct gaagccggcg ggagtagttg
     1801 gatgatgcag gagctctgga ggtgcaagaa agttggaggg gtaccctaac tgggcagaga
     1861 ccggctgcgg ctgtctcatg atgagcactg gcctggtgct ggtgatagcc ctcgggttga
     1921 tgcggctcat tgcgggctag ccggcggcca ccccggtcgt catcctcccg tgagtacgag
     1981 cgcggacgca ggctctcaga tctccccgcg ctcacctatc agcacggcac tgctcacgga
     2041 agtggtttag gccggagcgt gagggacatg aagagactct gcccatgctc caacgtcctt
     2101 ggtggatcgg caatcagagg cagcagcgtt aatagggctt ggaatgacgt gtcggcatgg
     2161 ttgtataagc tcggcgtgtc acagtcatgg agttgaggac aagtctacag acaaaccgta
     2221 gttggtaggg caagagggct tatcagtgat ttacaaagag tttaagggca taaccatctc
     2281 ccacctggga atgggcaata tgcgcctgcc gaccacgggt ccgggtggtc ttagggcgcc
     2341 gatagatcgg ggaaaggccc aggaaatcat cgactacgtc tatgcaaacg gggtcaatta
     2401 ctttgacacg gcatatatgt atcatggcgg cgaatcggag agattcctgg gcgaggctct
     2461 gaagaagtat ccgagagaga gctacctgct tgcggataag atgtggggca tgctggtcca
     2521 acaaggccgg ccgaccgctg acgtattcga ggaacagctg agacgctgcc agacggacta
     2581 tttcgatttc tatcttctcc acaatgtaag cgaaagcacc atcgacatct acatggatga
     2641 gaagctgggc atcctggacg acctgctgga acagaagaga accggacgga tccggaacct
     2701 cggactttcc agtcatggga aacctgcaac gctgaagcgt tttatcgagc ggtggcattg
     2761 tttcgacttt gtgcagattc aactcaacta tctggactgg acgcttcagg atgcgaaagg
     2821 ccagtatgaa gtcataacgg aacatggcat gcccgtgtgg gttatggagc cctgcagggg
     2881 tggccgtctc gcttccctca gtcccgaagc ggacgaggtt ctgaaaaagg ccaatccgaa
     2941 cgcttccatc gcctcttggg ctttccgcta tcttcagaca cttcccaatg ttcaggtcgt
     3001 tctcagtggg atgacgcaga tggaacaagc ggtggacaac attaggacat ttgcgcaccc
     3061 taatccactg acagaagaag agcaaaaggc attggaacag gctgtcagac tgctgaaaga
     3121 gagactgaat gtgccttgca ccaagtgccg ctactgtgac gggtgcccaa aggggttgga
     3181 tatccccgaa ctgttggcca tgtacaacga attatgcatc gatcgcggcg gcgcgttgat
     3241 gattgccctc ggcaatatgc agggcgaaaa gaagcctgca aactgcgttg cctgcggcca
     3301 gtgcgctaag aagtgtccgc agaacatcga cattccggga atcatgagca agtttgcgga
     3361 gacattggcc aagatgcctc ccacggcgct accaagatag agttccttgg cggaccgtcg
     3421 tcaatatcgc ctcggtcgag gcgcatacga tcctgaggga gtttccggcc tacgtcgcct
     3481 cgaagcatgg gttgatcggc ctgaccaagg gcaccgctta cgactacgcg gcacatggca
     3541 tccgcatcaa cagtgtctca cccggggtga ttcgtacccc tctgacgatg gccgaaggcc
     3601 agaaagaggt gaccgaccgc ctggcggtgc gcattcccca gggccggctg ggcgagcccg
     3661 aagaaatcgc ccgctcagtc gtcttcctgc tgtcggatct ctccagctac acgacaggcg
     3721 ccgatctggt cgtagatggt gggtttctac tgcgcgtgta atagtcgccg aagcagttgc
     3781 ccgacaggta gcgctggatg tgctgctgat cggcgggcgc ggaggcgtag agcttatcga
     3841 cgacatcgct cccaatgatc tgcttctgga ccgccagccc cttctcggcg cgggtctcgg
     3901 gcgtggtcgt ggattgaccc ggcagtggca atgcgacgcc ccggtcggtg agcacctcgt
     3961 tcgcggcgtg gatgaagtcg aagaccttgg ccatgccgac gtagggcacg gcctggtaca
     4021 cgatctccct gatctcgact ggtgtaaccc cgacagtgac cgccgcgccg accatgacgc
     4081 ggtactcgcg gagcgcctgg caggcgatca tcgaagcaag ctggaccatc agccgggtcc
     4141 gcacgtccag attgctgtgg cggagcactt cgtcaaaggc aaagttgtcg aagatctcga
     4201 tgagctcggg atcggtcccc gccagggtgg atacgtggcc ggggaaaagc ttgtcgtggt
     4261 tctttcgcgc tcgttcgttg ggcgccatgg tcgttctcct tgtcgtctgc ccgctgttgg
     4321 gctcgtagtt caatcaccac tcgcgtccgc tgtatgctca cttgaggccc acttgaaaca
     4381 cgcgagcgcc aatcagccat gaaggtgact gcgccggtcc aacagcgagc gagccacggg
     4441 gttacgcgcc caccttcggc agcgtagagt tgtcgatgac gaagcggaag cggacgtcct
     4501 tcgcgacggc cctgtcgtac gcctcgttga ccttgtcgat agggatgacc tcgatgtcgg
     4561 cggc
//
LOCUS       DATALS010000132         1970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_146400, whole genome shotgun sequence.
ACCESSION   DATALS010000132 DATALS010000000
VERSION     DATALS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146400"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VHS06_02865"
     CDS             complement(<1..143)
                     /locus_tag="VHS06_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2987093.1"
                     /translation="MAYGYTGKILRLNLTTGTISVDEHDDLWYRRYLGGAGIAAYYLL
                     NET"
     gene            372..1553
                     /locus_tag="VHS06_02870"
     CDS             372..1553
                     /locus_tag="VHS06_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2987094.1"
                     /translation="MGDVSSDLQEDIAGIKVAQAFNRTGVNQARFAQRNAANRDANVG
                     ATAVTSAFSPAMDILSTVATIIVVLFGGYLAIEGQVTVGLVVAFLGYVQQFFWPIQQM
                     SQIYTQAQSALAGGERIFELLDTEESMVDGLDARELPRIIGRVAFEKVDFAYDPMHPV
                     LHQVDLVAEPGQTVALVGPTGAGKTTVANLIARFYDVTGGRVSIDGVDVRDVTLHSLR
                     SQLGIVPQNSFLFSGTVADNLRYGRPDATDDEIERAARMANAHEFISRLPQGYNTMLG
                     ERGSSISQGQRQLLAFARAILAAPRILILDEATSSVDTRTEMLIQQALDQLLKGRTSF
                     VIAHRLSTIRNADQVLVIDDGRIVEQGTHSTLLEKGGLYADLYHRQFREIEEPETAPA
                     R"
     gene            complement(1691..1933)
                     /locus_tag="VHS06_02875"
     CDS             complement(1691..1933)
                     /locus_tag="VHS06_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX2987095.1"
                     /translation="MKVKVRVVGDFTKYPYPHWEWINVDTEPGITLQQFLEQVGIARG
                     EVGPATVNDALADMGTILREGDSVQAFSPVGGGSQR"
BASE COUNT          389 a          615 c          624 g          342 t
ORIGIN      
        1 ttggtctcgt tcagcagata gtaggcggcg atgccggcac cgccgaggta acgccggtac
       61 cagagatcgt cgtgttcgtc caccgagatc gtaccggtgg tgaggttcag acggagtatc
      121 ttgccggtgt aaccgtacgc catcgtttct ccatgctccc ttgcatacgc tgttctgccc
      181 acgccgcagc gaaaccggtc gaacgccgtg cgtgggaaac tctatctttg gactactatc
      241 ttagctctag aatcgattcc tgtcgccccc tttttcgacc cactatataa tgtcgacaag
      301 gaacgggcag gggctgaggc atggagggag cggagtgact ccgtggccac ccaacggatg
      361 cgcccggtgc catcggcgac gtctcttccg acctccagga ggacatcgca gggatcaagg
      421 tggcgcaggc gttcaaccgg acaggggtga accaggctcg tttcgcccag cgcaatgcgg
      481 ccaatcgaga tgccaacgtg ggcgcaacgg cggtcacctc cgcattttcc cctgccatgg
      541 acatcctgag caccgtcgcc acgatcattg tagtgctgtt cggcggctat ctggccatag
      601 aggggcaggt gaccgtaggg ctggttgtgg ccttcctggg ctacgtgcag cagttcttct
      661 ggccgatcca gcagatgagc cagatctaca cccaagcaca gtccgcgttg gccggcgggg
      721 agcggatctt cgaactgctg gatacggagg agtcgatggt cgacggcctc gacgcccgag
      781 agctgccgag gatcatagga agggtcgcat tcgagaaggt cgacttcgcc tacgacccga
      841 tgcaccccgt actccatcag gtggacctcg tcgccgagcc ggggcagacg gtggcgcttg
      901 tggggccaac cggggccggc aagaccaccg tcgccaacct gatcgctcgg ttctacgacg
      961 tgacgggcgg tagggtatcc atagacgggg tggacgtgag ggacgtgacc ctccactcgc
     1021 tgcggagcca actggggata gtgccgcaga acagcttcct cttctcgggg acggtggcgg
     1081 acaacctgcg ctacggccgg ccggatgcga cggacgatga gattgaacga gcagcccgaa
     1141 tggccaacgc ccacgaattc atctcacggt tgccgcaggg atacaacacg atgctggggg
     1201 agcggggtag ctccatatca caggggcagc gtcaattgct ggccttcgcg cgagcgatcc
     1261 tggccgcccc ccggatcctg atcctcgacg aggccaccag cagcgtggac acccgcacgg
     1321 agatgctgat ccagcaggcg ctcgatcaac tgttgaaggg gcggaccagc ttcgtcatcg
     1381 cccaccggct gagcacgatc cgcaacgccg accaggtgct ggtgatcgac gacggccgca
     1441 tcgtggagca gggcacccac tcgacgctgc tggaaaaggg cggcctctac gccgatctct
     1501 accacagaca gttcagggag atcgaggagc cggagaccgc ccccgcccgg tgagtgccct
     1561 ggcggaccgg cgagctaaag gcccgcgagg accaagacga acaggataga caggataaga
     1621 agagaagctc gctcgtccac tccgtactgc ggggcagatc tacaagggag ctcgggggct
     1681 caccgtgcac tcagcgctga gatccccctc cgaccgggga aaatgcctgg acggagtcgc
     1741 cctcccgcag gatggtgccc atgtccgcga gcgcgtcgtt gaccgtggcc ggaccaacct
     1801 cgccacgcgc tatcccaacc tgctccagaa actgttgcag ggtaatcccg ggctcagtat
     1861 ccacattgat ccactcccag tggggatagg ggtacttcgt gaagtccccg acgaccctga
     1921 ccttgacctt catatctcac tccaatggca ccgggcgcat ccgttgggtg
//
LOCUS       DATALS010000133         4059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_50703,
            whole genome shotgun sequence.
ACCESSION   DATALS010000133 DATALS010000000
VERSION     DATALS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50703"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..212
                     /locus_tag="VHS06_02880"
                     /pseudo
     CDS             78..212
                     /locus_tag="VHS06_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            288..1427
                     /locus_tag="VHS06_02885"
     CDS             288..1427
                     /locus_tag="VHS06_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987096.1"
                     /translation="MTAAYDYYELAHPDEGVGSGFVYDTIPHVTLESIAQNPEIREGL
                     EPEELEKVVLKYAPPEVLYDRPLIDGKKMRVSGPFTVEAVPAPTIKAPQQLLGEQPPI
                     LCSPEQHLVRMGESLRQDEWRSELLANGIRGKDGKRLRLSRIEPLGGTRWLQADADTA
                     GDDQVRAVFSFGSEYSPLEQRQVALALEEARTLVPRPAIIVFAAFQFDPEAAKDIDET
                     MWPGVTLLKVQMNTDLLADDLKKKRTGGDLFWMVGQPDVEVRPLPDAPVGDSWQVKVY
                     GFDYFDPTTGLVESGDESRGAMWMIDTDSDGRSLYPRQVYFPMTHPNDWERLARTLRQ
                     DLDSTLLDHFKGTVSLPFVAGQYRRAAVKIVDDRGIESLRILPLA"
     gene            1437..>4059
                     /locus_tag="VHS06_02890"
     CDS             1437..>4059
                     /locus_tag="VHS06_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX2987097.1"
                     /translation="MNSAIPQLIINSPYREPQKHWAYVRERQAFELRGGRRPAGYVVA
                     TPGSKSFDDPGVFHELPLVNRIRPRVSAWREAGYPGATGVSRRLLEHWWAADVREDRR
                     LFFCQLEAIETLMWLTEAPVAERQGIVVPSDGGPFTRYCAKMATGSGKTVVMAMLILW
                     QVLNKVAFPQDRRYSKHILVIAPGLTVKSRLQVLVPDHPDGDYYQAFDLVPSGLSESL
                     RQACVLIRNWHFLDWESDAQIARKHSVDKRGAKSDEAYVRDVLRGLGSARDLLVINDE
                     AHHAWRVPAESKVKGVTRGEIEEATKWVGALDRIHRVRSVAACFDFSATPYAPSGKQA
                     YEEALFGWIVSDFGLNDAIECGLVKTPRVVIRDDGQLGADYRSRLYHIYSDPEVKDDL
                     NRKAEPSEPLPDLVNTAYHLLGKDWLETERAWKAQGFPTPPVMITVANRTETAARVKY
                     AFDTGRVLISELQAPNKTVHIDSKVIDLAEAAEDPAVILSEEDEDGGEETEGGAVRKL
                     TKQERAEELRRVVDTVGKPGKPGEHIQKVISVGMLSEGWDAKTVTHVMGLRAFSSQLL
                     CEQVVGRGLRRTSYEVDEQTGLFTPEYVNVFGVPFAFMPHEGGEGSPPPPPVPKTLVE
                     VVASRHDLAISWPNVIRVEHIYSPQLQLDFTHVERLVIDATKIPTIAELAPVVNGKPD
                     LTMVSSIDLAEMGRIYRIQRLAFEAARDVFAQMRPAWPGSPEFLLAQLIGLVDQFLRS
                     DRIEIVPGLFFQDEQRRRVVLALSMTKIVQHIWDAIRAENTERLEVVLDAVKPVRSTG
                     DMLSWYTGRPCAPTCRSHISHCVYDSTWEASDAFRLERSPHVAAWAKNDHLGFDITYV
                     FKGVVHKYRPDYLVRLDN"
BASE COUNT          814 a         1138 c         1279 g          828 t
ORIGIN      
        1 gcttgaggtc atcggcgagt aggtcggtat tcatctgcac cttgagcagc gtcaccccgg
       61 gccacatggt ctcgtcgatg tccttggccg cctccggatc gaactggaag gccacaaaca
      121 cgaaggtcgt tgagcggtgc atccttctcg ccacggaccc cggcgacctc atcttcgacc
      181 ctacctgtgg ctcgggcacg acggcgtact gcgcggagaa gtggggtcgt cgctggataa
      241 cctgcgatac atcgcggatt gcggtgactg tggcgcggca gcgcttgatg acagccgcct
      301 atgactacta cgagctagcg cacccagacg aaggggtggg gagcggcttc gtgtacgaca
      361 cgattccgca cgttaccctc gagtctattg cccagaatcc ggagatccgc gaaggcttgg
      421 agccggagga actggagaag gtggtgctta agtatgctcc acccgaggtt ctctacgatc
      481 gaccattgat cgatggcaag aagatgcggg tgtccggacc gttcacggtg gaagcagtgc
      541 ccgcgcccac catcaaggcc ccccagcagc ttcttggtga acaacctccc atcctttgca
      601 gtccggaaca gcaccttgtg cggatgggag aatcactgcg acaggatgag tggcggagtg
      661 aactcctggc caatggcatt cgcggcaagg acgggaagcg gctgcgcctg tctcggatag
      721 agccacttgg tggtacgcgc tggctgcagg ctgatgcgga tacggcggga gacgaccaag
      781 tgcgggccgt attctcattc ggctcggagt actctccgct ggaacagcgc caggtagcgc
      841 tggcgcttga ggaggcacgg accttggtgc ctcgcccagc aatcattgtg tttgcggcct
      901 tccagttcga tccggaggcg gccaaggaca tcgacgagac catgtggccc ggggtgacgc
      961 tgctcaaggt gcagatgaat accgacctac tcgccgatga cctcaagaag aaacgtaccg
     1021 gcggcgatct gttctggatg gttggccagc ctgatgtaga ggtgcgtcca ctccccgatg
     1081 ctcctgttgg cgactcatgg caagtcaagg tctatggttt tgactacttt gaccccacga
     1141 cggggctcgt cgaatccgga gatgagagca ggggggcgat gtggatgatt gatacggact
     1201 ctgacgggcg gtctctgtat cctcggcagg tgtacttccc catgacacat cccaacgact
     1261 gggaacgcct ggcgagaacg ctgcgccagg atcttgactc gactctcctg gatcatttca
     1321 agggcacggt gtcgctaccc ttcgtcgcgg gtcagtatcg gcgggcagcg gtcaagattg
     1381 ttgatgaccg cggtatcgag agtctacgca ttctgccgct cgcttaggcg gaacccgtga
     1441 acagcgcaat acctcagctg atcatcaact cgccgtaccg ggagccgcag aagcattggg
     1501 catacgtgcg ggagcgtcag gcgtttgagc tgcgcggcgg ccgccgcccc gcagggtatg
     1561 tcgtagccac tcccggttcc aaatccttcg acgacccggg cgttttccac gagctgcctc
     1621 tggtcaaccg aatccgtccg cgcgtgagtg cgtggaggga agcaggctac cctggggcga
     1681 caggggtcag caggagactg cttgagcact ggtgggcagc cgatgtcagg gaggatcggc
     1741 gtctcttctt ctgccaactc gaggccatag agacgctgat gtggctcact gaggcaccgg
     1801 ttgctgagcg gcagggaatt gttgtaccgt ccgatggcgg tcctttcacg cgctactgtg
     1861 ccaagatggc gactgggtca ggcaagacgg tcgtgatggc catgttgatc ctgtggcagg
     1921 tgctcaacaa ggttgccttc ccccaggacc gtcgctactc caagcacatc ctggtaattg
     1981 cgcctggcct taccgtcaag agccggttgc aggtgctggt gcctgatcat cctgatggcg
     2041 attactacca agcgttcgat ctcgttccga gtggtctcag cgagtcacta cggcaggcgt
     2101 gcgtgcttat ccgcaactgg catttcttag attgggagtc tgacgcccag atagccagga
     2161 agcacagcgt tgacaaacga ggagccaaga gcgacgaggc ctacgttcgt gacgtgttgc
     2221 gcggcttggg ctcggcgcgg gatcttctgg tcatcaacga tgaggcccac catgcctggc
     2281 gtgtccccgc ggaatcaaaa gtcaagggcg tgacgcgggg agagatcgaa gaggccacca
     2341 agtgggtggg ggcgcttgac cgtatccata gagttcgttc cgtcgctgcc tgcttcgact
     2401 tctccgccac accctacgcc ccttcaggta agcaagctta cgaagaggct ctgttcggct
     2461 ggatcgtgag cgatttcggt ctgaacgacg cgatcgagtg tggtctggtc aagacccctc
     2521 gagtagtcat ccgagacgac ggccagctgg gagcggacta ccgatctcgc ctgtaccaca
     2581 tctactccga tccagaggtg aaagacgatc tcaaccggaa agcggaaccg agtgaacccc
     2641 tgcccgacct tgtgaatacc gcatatcacc tccttgggaa ggattggctc gaaaccgagc
     2701 gggcatggaa ggcgcaaggg tttcctaccc cacctgtcat gatcaccgtt gcgaaccgga
     2761 cggaaaccgc tgcgcgagtg aagtacgctt tcgacactgg tcgtgtgctc atcagcgaac
     2821 tgcaggctcc gaacaagacg gtgcacattg attcgaaagt cattgacctc gccgaggccg
     2881 ccgaagaccc cgcggtcatc ctctcggagg aagatgagga tgggggcgaa gagactgagg
     2941 gtggtgcggt caggaagctc accaagcaag aacgtgccga ggagctgcgc cgagttgtag
     3001 acacggtggg caagccaggc aagccgggag aacacatcca gaaggtaatc tctgtgggta
     3061 tgctctccga aggatgggac gcgaagacag tgactcatgt catggggctt cgcgcattct
     3121 cgagtcagtt gttgtgcgag caggttgtgg gtaggggatt gaggcgtact tcctacgaag
     3181 tggacgagca gacagggcta tttactcccg agtacgtgaa cgtatttggg gtgccgtttg
     3241 ccttcatgcc gcacgagggt ggagaaggga gtcctcctcc gccacctgtc cccaagaccc
     3301 tggtcgaagt tgtggccagt cggcacgact tggccatctc gtggccaaat gtcattcgtg
     3361 tggaacacat ctactctccg cagctgcagc ttgacttcac tcatgtcgaa cggctggtca
     3421 tcgacgccac taagatacca acgatagctg agttggcccc ggttgtaaat ggcaagccag
     3481 atttgaccat ggtgagttcg attgatttgg ccgagatggg acgaatctat cgtattcaga
     3541 gactggcctt cgaggcggct cgtgatgtct tcgcgcagat gaggcccgcc tggccggggt
     3601 ctcccgaatt ccttcttgcg cagctaatcg gcctcgttga ccagtttctt cgttctgacc
     3661 ggatcgaaat cgtgcccggc ctgttctttc aagacgaaca gaggcggcgg gtcgtgctgg
     3721 cgttgagcat gaccaagatt gtgcagcaca tctgggatgc cattcgagcc gagaacacgg
     3781 agaggcttga agtggtgctg gacgccgtga agcccgtgcg gtcaaccggg gacatgctca
     3841 gttggtacac gggtcggcct tgcgctccaa cgtgccgttc tcatattagc cactgtgtgt
     3901 acgacagcac ctgggaggcg agcgatgcct tccgcctgga acgcagcccg catgtggctg
     3961 cgtgggcaaa gaatgatcac ctcggcttcg acatcactta tgtcttcaag ggtgtcgtac
     4021 acaagtaccg ccccgactac ctcgtgcggc tggacaatg
//
LOCUS       DATALS010000134         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_50861,
            whole genome shotgun sequence.
ACCESSION   DATALS010000134 DATALS010000000
VERSION     DATALS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50861"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VHS06_02895"
     CDS             complement(<1..626)
                     /locus_tag="VHS06_02895"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEX2987098.1"
                     /translation="MVRIFGRQDPGASHGTPSEGDRATQCSACGREVGGDPLFARFKV
                     CPFCGFHHSLSAYERISLLADPGSFKETLANLYPTDPLGFVDRVPYAQRLEEARQKTG
                     LADAAVTGTALIEGQAAVLIVLDFRFLGGSMGSVVGEKVASACELAIKKKTPLVAVTC
                     SGGARMQEGMLSLAQMAKTSAAVERVHAAGVPFFVLLCRPLRTMRSCRR"
     gene            complement(775..1848)
                     /locus_tag="VHS06_02900"
     CDS             complement(775..1848)
                     /locus_tag="VHS06_02900"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX2987099.1"
                     /translation="MAYRIAVIPGDNIGPEVIREGLKVLGALQRVGYGPYEFTEFPWG
                     AGYYLKTGRAMDEDGIERLKAFDAIYFGAHGDPARVPDVVSSQGLIHPLRRSFDLYVN
                     VRPARLWPGVVSPLRNKDEIDFVVVRENSEGEYSGMGGRLHRGTPLEVATMVSVVSRA
                     GAERLIRFGFELALKRNGKKRVAAATKSNALGHTMSLWDEVFLKVAAEYPDIEAVKYH
                     VDAFSMYLIQRPEWFDVIVATNMFGDILSDEGAAITGGVGLAPGANLDPERRFPSMFE
                     PIHGSAPDIAGKNVGNPLATILAGAMMVDWLGEEEASELVMRAVEEVMREGRVRTRDL
                     GGHSNCSDMGDAVAEKVARLAAK"
BASE COUNT          396 a          710 c          569 g          355 t
ORIGIN      
        1 cgccgacatg agcgcatcgt ccgaagcggg cggcacaaca gcacgaagaa gggcacccct
       61 gcggcatgaa ccctctccac ggcggcggag gtcttcgcca tctgtgccag ggagagcatc
      121 ccctcctgca tccgcgcacc accactgcag gtaaccgcca ccaacggcgt cttcttcttg
      181 atggccagct cgcatgcgga cgccaccttc tccccgacga cggagcccat gcttcctccc
      241 aggaagcgga agtccagcac gatcagaacg gcggcctgac cctctatcag cgcggtgccg
      301 gtgacggcgg catccgccag cccggtcttc tgtcgagcct cctcgagccg ctgcgcgtag
      361 ggcaccctat cgacaaaccc cagggggtct gtgggataga ggttcgccag ggtctccttg
      421 aagctaccgg gatcggcgag gagggagatc cgctcgtagg cggacaatga gtggtggaag
      481 ccacagaacg ggcatacctt gaagcgtgca aacaggggat cgccgccgac ctcccgaccg
      541 caggcgctgc actgcgtagc gcggtcgccc tccgaagggg tcccgtggga tgcccccgga
      601 tcttgccggc caaagattct caccattttc gccccgtacc ctttcccgcc ggcactgctc
      661 tgtcgccggc gcagtgatgt ggatcggtcc catcgggggt tcgaggtatc cacatagtat
      721 cgcgatatat cgcgatacta tgtcaggctt ttggggatgt caatagccca agagctattt
      781 cgcggccagc cgggccacct tctcggccac cgcgtccccc atatcggaac agttggagtg
      841 cccccccagg tcccgagtcc gaacccgccc ctcccgcatg acctcttcta ccgcccgcat
      901 caccagttcg gaagcctctt cctcccccag ccaatccacc atcatggcac cggccagaat
      961 agtagccagc gggtttccga cgttcttccc ggcgatatcg ggggcggagc catggatcgg
     1021 ctcgaacata gagggaaacc tcctctccgg atccaggtta gcacctgggg caagccctac
     1081 ccctcccgtg atggccgccc cctcgtccga gaggatgtcc ccgaacatat tggtcgccac
     1141 gatcacgtcg aaccactcgg gacgctggat cagatacatg gagaaggcat cgacgtggta
     1201 cttcacggcc tctatatcgg gatactcggc cgcgaccttc aggaagactt cgtcccagag
     1261 ggacatcgtg tggccgaggg cattggactt cgtggcggcg gcaacccgct tcttgccgtt
     1321 ccgcttgagt gccagctcga agccgaagcg gatcagccgc tcggcgccgg ctctgctgac
     1381 cacgctgacc atggtggcga cctccagggg cgtgcctcga tgcaaccggc cgcccattcc
     1441 ggagtactcg ccctcgctat tctcgcggac gacgacgaaa tcgatctcgt ccttattccg
     1501 cagcggagaa accaccccag gccagagccg agcggggcga acgttcacgt agaggtcgaa
     1561 ggacctgcgc aatggatgga tcaacccctg ggacgacacc acgtccggaa cccgggcggg
     1621 atcgccatga gctccaaagt agatggcatc gaacgccttg agtctctcga taccgtcctc
     1681 gtccatcgcc ctgccggtct tcagatagta cccggccccc cagggaaact ccgtgaactc
     1741 gtaggggccg tatccaaccc gctgaagcgc ccccagcacc ttcagccctt cccgaatcac
     1801 ctctggcccg atgttgtctc cgggtatgac cgcgatccga taagccaccg ctaccccacc
     1861 tctccatcag cttcgaatcg acccccaggt atgtccgtac ccactgatat gtacaccaat
     1921 atgtacaact tgggaagccc cactcgagcc gtttcacgcg gtcacagcac cacaggcacc
     1981 gactcggata gctctatagc atctggcgac acgcgacagc ggaaggccaa
//
LOCUS       DATALS010000135         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_146954, whole genome shotgun sequence.
ACCESSION   DATALS010000135 DATALS010000000
VERSION     DATALS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146954"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1468
                     /locus_tag="VHS06_02905"
     CDS             2..1468
                     /locus_tag="VHS06_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEX2987100.1"
                     /translation="MGAVDPRLYGSFVEHLGRAVYGGIYEPGHPAADEDGFRQDVLDL
                     VRELGVPVVRYPGGNFVSGYDWEDGIGPKERRPRRLDLAWRCLETNQFGTDEFIAWCR
                     KAGTEPMLAVNLGTRGVDEARCLVEYCNHPDGTFFSDLRKANGVEKPHGVKFWCLGNE
                     MDGPWQIGQKSSDEYGRLARETAKAMRLVDPSIELVACGSSNSGMPTFGSWEYTVLDH
                     CYDLVDYISLHTYYGNPSGDTPNFLAKSLDMDRFIETVVSICDAVKGRKRSRRRINLS
                     FDEWNVWFHTRESDREMKPWQVAPSLLEDCYTFEDALLVGCMLISLLKHADRVKIACL
                     AQLVNVIAPIRTVTNGGCWRQTVYYPFFHASRFGRGTALAVNPVSPGYDDKEFGAVPY
                     LEAVATHDAEEHCLTLFAVNRSMDQELRLDGDIRGFPGCSVQEHITMTHADPLATNTL
                     DAPERVVPGRGGDAAVRDGRLEVTLPRLSWNVIRLAHR"
     gene            1582..2211
                     /locus_tag="VHS06_02910"
     CDS             1582..2211
                     /locus_tag="VHS06_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2987101.1"
                     /translation="MAIAAEFERGGRELVVLDPHMLIPMPRPHCLADAELLPRIALSM
                     TGGRRASLILANGNGEVWLEAGVGVGLPAGQSLSLGPGIVSRVLETRQPVVVQDLSET
                     SFPAHPERGYETRSCMAVPVLFGTECHGVMCVSDPSRSQVFTADDFESLKLLTDHIGT
                     TIRSMLSLQAQVNLDNLTGGAESQDLRGAPGGGSGEGEALRAFSVDAAC"
BASE COUNT          433 a          643 c          761 g          477 t
ORIGIN      
        1 gataggggcg gtggatcccc gactgtacgg gtcattcgtg gaacacctgg ggcgagccgt
       61 gtacggtggc atctatgagc ccgggcatcc cgccgcggac gaggatgggt tccggcaaga
      121 cgtgctggac ctcgtcagag agttgggcgt gccggtagtg cgctatccgg gaggcaactt
      181 cgtttcgggc tacgactggg aggacggcat cgggccgaag gaacggcggc cccgccggct
      241 ggatctggcc tggcgctgcc tggaaacgaa ccagttcggc actgatgagt tcatcgcctg
      301 gtgtcgcaag gcagggacgg agccgatgct ggccgtgaac cttggcactc ggggggtgga
      361 cgaagcccgg tgtctggtcg agtactgcaa tcatcccgac ggaaccttct tcagcgatct
      421 cagaaaggcc aacggcgtag agaagcctca cggagtcaag ttctggtgcc tggggaacga
      481 gatggatggt ccatggcaga tcgggcagaa gagctccgac gaatatggcc gtctggctcg
      541 ggagaccgcc aaggcgatgc gattggtgga ccccagcatc gagttggtgg cttgcggcag
      601 ctccaacagc ggcatgccca ccttcgggtc atgggagtac acggttctcg atcactgcta
      661 cgacctggtg gattacatct ccctccacac ctactacggc aacccaagcg gtgatacacc
      721 caatttcctc gccaagtcgt tggatatgga ccggtttatc gagacggtgg tgtccatttg
      781 tgacgcggtc aagggcagga agaggagccg aaggaggatc aacctctctt ttgacgagtg
      841 gaacgtttgg ttccatacta gagagtcgga tcgggaaatg aagccatggc aggttgctcc
      901 ttcgctgctg gaggactgct acaccttcga ggacgcgctg ctggtggggt gcatgctgat
      961 ctccctgctg aagcacgccg acagggtcaa gatcgcctgc cttgcgcagt tggtcaacgt
     1021 cattgcacct atccgtaccg tcacgaacgg agggtgttgg aggcaaaccg tctactatcc
     1081 tttcttccac gcttcgcgct tcggccgcgg taccgccctg gcggtgaatc cagtctcccc
     1141 tggctacgac gacaaagagt ttggtgccgt cccctacctg gaggctgtgg cgacccacga
     1201 tgcagaggaa cactgcctca cgcttttcgc cgtgaatcgg agcatggatc aggaattgag
     1261 gcttgatggc gacattaggg gcttccctgg gtgttcggtg caggagcaca tcaccatgac
     1321 ccacgccgat ccgctggcga ccaataccct ggacgctcca gaacgcgtgg tgcctggtag
     1381 gggcggggat gcggctgtgc gagacggcag actggaagtc acgctgccgc ggctgtcctg
     1441 gaacgtgatc cggttggcac accgataatt cgccttgaga gaggctaaag aagatttgga
     1501 aaacagccga tacttcttat gtgatcggat tctgtctctc ttgtccgatc gttcgtgcgt
     1561 tgccttgctg gaggggatcc tgtggcgata gcagccgagt ttgagcgggg tgggcgtgaa
     1621 ctggtggttc tcgacccgca catgctcatc cccatgcctc ggccacactg tttggccgat
     1681 gccgaactgc ttccacggat agctctgtcc atgaccggtg ggcgcagggc atcgctgatc
     1741 cttgccaacg ggaacggtga ggtgtggctg gaggccggtg ttggagtcgg cctcccggct
     1801 ggacagtcgc tgtctctcgg ccccggcatc gtttcgcggg ttctcgagac gcgtcagccg
     1861 gtggtggtcc aggatctctc cgagacatca tttcccgcgc accctgaacg cggttacgag
     1921 acccgatcct gcatggccgt cccggtgctc ttcggaacag agtgccacgg ggtgatgtgc
     1981 gtttcggacc cttctcgctc ccaggtcttc actgcagatg acttcgagtc cctcaagttg
     2041 ctgacggatc acatagggac caccatccgg tcgatgcttt cactgcaggc ccaggtgaac
     2101 ctcgacaacc tgacgggggg tgcagaatcg caggaccttc gaggagcgcc tggcggtgga
     2161 agtggagagg gcgaggcgct tcgggcgttc tctgtcgacg ctgcttgttg acataaacag
     2221 tctaggcagg atcaacgagc gtgacggcca actggtggga gaccaggttc tccagtggat
     2281 tgcgcgggaa atgatgtctc ggagagatcc tgga
//
LOCUS       DATALS010000136         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_193228, whole genome shotgun sequence.
ACCESSION   DATALS010000136 DATALS010000000
VERSION     DATALS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193228"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1303
                     /gene="tgt"
                     /locus_tag="VHS06_02915"
     CDS             122..1303
                     /gene="tgt"
                     /locus_tag="VHS06_02915"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX2987102.1"
                     /translation="MFDFTLQERCAGTAARTGVLTTPHGPMLTPAFMPVGTQATVKSL
                     SPDELRDLGAQCILSNSYHLHLRPGSGLVAKMGGLHRFMGWDRAILTDSGGFQVFSLA
                     HLRRISEDGVAFRSHIDGSEHFFSPESVMRIEEELGADIIMAFDECAPYPAEREYVFQ
                     AMERTHRWAERCLKAHTRPDQALFGIVQGGTHPDLREQSSKTLSAMGFKGFGIGGLSV
                     GEPKEVMYSVLEQTTVHLPEDRPRYLMGVGSPEDLVECVARGVDMFDCVLPTRIARNG
                     ALFTHSGRLNIRNARYQDDPLPIEEGCGCYCCRNFSRAYLRHLIKSDELLGLRLTTIH
                     NVHFLLQLAREIRASISNGSFPTLRERFLAGYTPVEEEARAANRAARAAAFEADRIGH
                     E"
     gene            1516..>2138
                     /locus_tag="VHS06_02920"
     CDS             1516..>2138
                     /locus_tag="VHS06_02920"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX2987103.1"
                     /translation="MRISPPSVGRQLQIDEESGSNHTHRPTAQTTAWVVLSVAFGIFV
                     VMVIGAFVGAKYYYDTATEPRTARLSIEEGIVLFRDAVSSTLINAPNDFELREGDGLL
                     VGQGARAAILLDDGSKVRLYSGSEMKIDGLRKSRFHRGSTTVSLGLDKGTARLEISPP
                     QADSSEFLITSPYGYALLDRGNYGVEVSEGRTRISVRDGIATAYGKSG"
BASE COUNT          445 a          657 c          631 g          405 t
ORIGIN      
        1 cgcaactaca tctcccggaa cattgcggct agacatggta gaatagctta agcctctcgt
       61 gcggggcctt ccaggccccg caccccattt gtccgaaagc attcccgcac caggaaccgc
      121 cgtgtttgac tttaccttac aagaacgttg cgccggcact gcggccagaa cgggggtact
      181 caccacccct cacggaccga tgctcacccc tgccttcatg ccggtaggaa cccaggctac
      241 cgtcaagtcg ctcagcccgg atgaacttcg cgacctggga gcacagtgca tcctttccaa
      301 cagctaccac ctccatctgc gtccaggttc tggcttggtg gccaagatgg gtggtctgca
      361 caggttcatg ggttgggaca gggccattct caccgattcg ggtggcttcc aggtgttcag
      421 tctcgcccac ctccgccgca tcagcgagga cggggttgcg tttcgatccc acatcgacgg
      481 ctccgagcac ttcttctcac cggaaagcgt catgcggata gaggaggagt tgggcgcgga
      541 tatcataatg gccttcgacg aatgcgctcc ctatcccgcg gaaagagagt acgtgttcca
      601 ggcaatggag cgcacacacc ggtgggcgga gcgctgcctg aaggcccaca cccgccccga
      661 ccaggccctg ttcgggatcg tccagggcgg cacccacccg gacctccgag agcagagctc
      721 caaaacgctc tcggcgatgg gtttcaaagg gttcggcatc ggcggcttga gcgtgggtga
      781 gcccaaggag gtcatgtact ccgtcctgga gcagacaacc gtgcacctcc cggaggaccg
      841 tccccgctat ctgatggggg ttggctctcc cgaggacctg gtggaatgcg tggcccgtgg
      901 ggtcgacatg ttcgactgcg tcctccccac ccgcatcgcc cgaaacgggg cgctcttcac
      961 acatagcgga aggttgaaca tccgcaacgc tcggtaccag gatgatccgc ttcccataga
     1021 agaggggtgc ggctgttact gttgtcgcaa cttctcgagg gcctacctgc gccacctgat
     1081 caagtccgat gaactcctgg gcctcaggct caccaccatc cacaacgtgc acttcctgct
     1141 gcagttggcc cgcgagatac gcgcctccat atccaacggc agtttcccga cactcaggga
     1201 acggttcctg gcagggtata ctccggtgga agaggaggct agagcggcaa atagggcagc
     1261 gcgtgcagcg gccttcgagg cagatagaat agggcacgag tagcccgtga ctttacagtc
     1321 tacggctctc ttgctacact cgctataggc ttagtctttg gaggtgaatg gtcatgggaa
     1381 accgcgacaa gcgagacgac aaggtcaaga aaccgaagaa agatgcaaag ctctcgacca
     1441 tacactccca ggtcacagag gttccgccac cggttgagat agtacggaaa cggcgaaagg
     1501 acgaatctgg ggataatgcg gattagtccg ccgtctgtcg gccggcagct tcagattgat
     1561 gaagagtctg gatccaacca cactcacaga cccactgccc agaccacagc ctgggtggtc
     1621 ctgtccgtag ccttcggcat cttcgtggtg atggtcattg gagccttcgt aggggcgaag
     1681 tactactacg atacggctac cgagccacgc acagccaggc tctccatcga agaaggcata
     1741 gtcctcttcc gcgatgcggt ttcgtctacc ctcatcaacg caccaaacga cttcgaactg
     1801 agggaggggg atgggctgct cgtcggacag ggtgcgcgtg ccgccattct gctggacgac
     1861 ggcagcaagg tgcggctcta ctccggttcc gagatgaaaa tcgacggact gcgaaagagt
     1921 cgttttcatc ggggctctac taccgtctcg ctcgggctgg acaaggggac tgccaggctc
     1981 gagatctccc ctccccaagc cgacagcagc gagttcttga tcacctcacc gtacggctat
     2041 gcactcttgg acaggggcaa ctacggcgtg gaggtatccg agggtaggac tcgcatctcc
     2101 gtccgggacg gcatcgcgac ggcctacggc aagagcgg
//
LOCUS       DATALS010000137         5340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_147127, whole genome shotgun sequence.
ACCESSION   DATALS010000137 DATALS010000000
VERSION     DATALS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147127"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VHS06_02925"
     CDS             <1..199
                     /locus_tag="VHS06_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987104.1"
                     /translation="PVQEGPRILFGDATDLDGKLSSLAAIREHLDSTKTKADVIDLRF
                     KGRPVYVVPPSVPAGKQGRTR"
     gene            292..657
                     /locus_tag="VHS06_02930"
     CDS             292..657
                     /locus_tag="VHS06_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEX2987105.1"
                     /translation="MWLPLLGLLTGVVIGLSFSISIPADYARYTGMAILAAMDSILGA
                     IAAELDGRYENDIFVAGLFTNMLLAGTLTFLGDRLGVELYFAAIVAFGARIFNNLAIA
                     RRHFVGRLRQWQRLRAGQG"
     gene            660..1889
                     /gene="ftsA"
                     /locus_tag="VHS06_02935"
     CDS             660..1889
                     /gene="ftsA"
                     /locus_tag="VHS06_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX2987106.1"
                     /translation="MADEQVVVGLDVGTSKVSALIGEVKRPDLVQVVGVGISPSRGLK
                     KGLVVDMDEAAAAIAASLRKAETFSGYKIIGAFVGVSGAHLESDMIHGAVSLPGDRTV
                     SQQDLLEVQRAARLEDVAGGRHVLHVLPRGYVVDGEDGVRNPAGMVASVLEMEGLMVT
                     AALAPIQNLIRCVDRAGIKIDGLVASGPASAQGVLSDAEKKLGVLLMDLGGGTTDLTW
                     FKDGEVQFVGALPLGGNHLTNDISVGLGVPFSAAEEMKVRFGHAVPGNVGEDETIDAT
                     NSSEEHPRKVSRRFLAEIIEARLSEIFGLAMQELEHRGYDGVLPGGVVLTGGTAQLRG
                     VRELAQQTFDMPVRLGLPEGLAGSIDSISTSAYATGAGLLKWVLSQPDEVGGGRLMRR
                     RVGWGGRLKGLAKAFLP"
     gene            1979..3064
                     /gene="ftsZ"
                     /locus_tag="VHS06_02940"
     CDS             1979..3064
                     /gene="ftsZ"
                     /locus_tag="VHS06_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015139802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX2987107.1"
                     /translation="MEREGVPRIIVVGAGGGGGNAVNRMIQAQVRGVEFVAVNTDQQA
                     LARTERGATRLRIGDKITRGLGSGGNPTIGAKAAEESVEEIYQLLNGADMVFITAGMG
                     GGTGTGASPVIAQVAKELGALTVGVVTKPFIFEGSRRRQVAEEGAAALKSKVDTLITI
                     PNDRLLQVIERNTPVESALRVVDDVLRQGIQGISELITEPGLINLDFADVRAVMSNAG
                     SALMAIGRAKGENRAAEAAKSAIANPLLDVSMTGAKGVLFNVTGGNDLTLNEINDAAE
                     VVFAAADQEANIIFGAVVDERIEDEIRITVIATGFDGTITSASPIERVDRIRPVPSQP
                     AVATRPLEKVERSDYDVPAFLRRRQER"
     gene            3194..3988
                     /gene="pgeF"
                     /locus_tag="VHS06_02945"
     CDS             3194..3988
                     /gene="pgeF"
                     /locus_tag="VHS06_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX2987108.1"
                     /translation="MSDRLLESIAFYPFDIFAPWPELTAGAFGRSGGVSPAPYDSLNI
                     SYAVQDDPERVRTNRELMARAVGWDPGRIVACRQVHGTHVVAATSEMAGGPELQGVDA
                     LVTNEPGILLMLKFADCVPIVLWDPVRRVVAVVHAGWRGTVARVPAAALHFMVTQYGS
                     EPSDVRAGIGPSIGPCCYQVGQEVATKAEQVFTGTGVVSPGPDGTPHFDLWSANAETL
                     MRSGVAEENVAISRICTRCRSDLFFSHRALGSPSGRFAMVGGIRDV"
     gene            3981..4688
                     /locus_tag="VHS06_02950"
     CDS             3981..4688
                     /locus_tag="VHS06_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX2987109.1"
                     /translation="MCSLGQRLEDVHRRIADAAERAGRSPEDIQLVAVSKTVPAETVM
                     EAYRLGQRHFGENRVQEFREKRDALAELGPMPDAVWHMIGHLQRNKAKPCVELFDIIQ
                     SVDSVKLAGLLNADAGVAGKRLRILLEVDFSDESQRSGFSPVELERVAGELVLLPHVE
                     IQGLMTVAPLGLDQEGTRTVFRRLRQLRDRLATCYPAVSWRHLSMGMSDDYVLAIEEG
                     ATIVRVGRAIFGERSRG"
     gene            4715..4975
                     /locus_tag="VHS06_02955"
     CDS             4715..4975
                     /locus_tag="VHS06_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX2987110.1"
                     /translation="MLFLYRFVDLLFNILIFAIVGRALLSWFNLGPGNPIVRLLYEIT
                     EPILAPMRRVIPMIGMLDISPIVAILLLSFMQNLILQAMVAY"
     gene            complement(5161..>5340)
                     /locus_tag="VHS06_02960"
     CDS             complement(5161..>5340)
                     /locus_tag="VHS06_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987111.1"
                     /translation="TDRHIQPSLGYINADKVGFLIHRSLLQMAHPCMMRALMPWQLFG
                     LMMRGRGDQRYVSVF"
BASE COUNT          962 a         1457 c         1838 g         1083 t
ORIGIN      
        1 tcctgttcag gaggggccgc ggatactgtt tggggatgcg accgacctgg acggaaagct
       61 gagcagcctt gcagcgatcc gcgagcatct cgactcgacc aagactaagg ctgatgtgat
      121 cgatctccgg ttcaagggcc ggccggtata cgtagtccca ccttccgttc ctgcggggaa
      181 gcagggccgc actcgctgac aaggcgaggg atgggcagcg cgctcgccgg cgcggagtcg
      241 gtgaggcagc actggcggtt ttcgccagca tccgtgcgga gggtatctcc aatgtggctt
      301 cctctactcg gtctcttgac tggagtggta ataggtctct ccttctccat ctcgataccc
      361 gcggactacg ctcggtacac tggcatggcc atcctggcgg cgatggactc catcctgggt
      421 gctatcgcgg ctgagttgga cggcaggtac gagaacgaca tatttgttgc tgggctcttt
      481 accaacatgt tgctggcggg tacgcttacc ttcctgggcg acagactggg tgtggagctc
      541 tacttcgccg ctatagtggc gttcggtgct cgaatcttca acaacctcgc catcgctcgg
      601 cgccattttg tcggcaggct cagacagtgg caaaggctga gggcgggcca ggggtaagta
      661 tggctgatga acaggtagtt gtcggcctgg acgtcggcac gtcaaaagtc agtgcgctca
      721 taggtgaggt gaagcgaccc gacttggttc aggttgtagg ggttgggata tccccatccc
      781 gcggcctgaa gaaggggttg gtggtggaca tggatgaggc cgcggcggct atcgcggcct
      841 cgctgcgcaa ggcagagacc ttctccggct acaagatcat tggcgccttc gttggggttt
      901 caggcgccca tctggagtcg gatatgatcc acggtgccgt gtcgcttccg ggagacagga
      961 ctgtatctca gcaggacttg ctggaggtgc agcgagctgc tcggctggag gacgtggccg
     1021 gtggacggca cgtgctccac gtgctcccca ggggctatgt ggtagacggt gaggatgggg
     1081 ttcggaaccc cgctggaatg gttgcctcgg ttctggagat ggaggggctg atggtcactg
     1141 cggccctggc tcccatccag aacctgatcc gctgtgtgga tcgggcgggg attaagatag
     1201 acggtctggt ggcgtcgggg cctgcctctg cgcagggggt attgagcgac gccgagaaga
     1261 agttgggtgt cctgctgatg gacctgggcg gcgggacgac cgacctgacc tggttcaagg
     1321 acggcgaggt ccagttcgtg ggcgcacttc ccctgggggg aaatcacctg accaacgaca
     1381 tctccgttgg tctgggtgtg cccttttccg cggctgagga gatgaaggtt cgtttcggcc
     1441 acgccgtgcc cgggaatgtg ggcgaggacg agacgatcga cgcgaccaac tcctctgagg
     1501 agcaccctcg caaggtctcc aggcgtttcc tggcagagat catagaggct cggctttccg
     1561 agatctttgg tctggcgatg caggagttgg agcatcgagg gtacgatggg gtgttgcccg
     1621 gtggcgtcgt gcttaccggc gggactgcgc agctgcgggg ggttcgcgag ttggcgcagc
     1681 agactttcga catgccggtg aggctggggt tgcctgaggg tctggcaggt tccatagact
     1741 ccatcagcac ctcggcctac gccaccggtg cggggctgct gaagtgggtg ttatcgcaac
     1801 ccgacgaggt cggtggcggc agattgatgc gacgtcgagt aggctggggt ggcaggctca
     1861 aagggctggc caaagcattt ctgccctagg tccaaggatg ggccgcgccg atatccttcc
     1921 ccaggtgtat cggacagggc tgagaggtgg ggaagtggtc gtcaataggg ggcaacaggt
     1981 ggaaagggaa ggcgtacctc ggataatcgt cgtcggtgcc ggcgggggcg gcggcaatgc
     2041 ggtcaaccga atgatccagg cccaggtgcg tggcgtggag ttcgtggccg tcaatacgga
     2101 tcagcaggcc cttgccagaa ccgagcgcgg cgcaactcgg cttaggatcg gcgacaagat
     2161 tacccgcggc ctgggctccg gaggcaaccc gaccataggc gcgaaggctg ccgaggagag
     2221 cgttgaggag atctatcaac ttctcaacgg ggcggacatg gtcttcatca ctgccggtat
     2281 gggtggcggt accgggaccg gcgcctcgcc ggtgatcgcc caggtcgcca aggagctggg
     2341 tgccctcacc gttggggttg tgaccaagcc cttcattttc gaaggctcgc gtcgccgcca
     2401 ggtggctgag gagggtgccg ccgccttgaa gtcgaaggtg gacacactga taaccatccc
     2461 caacgatagg ctgcttcagg tcattgagcg caacaccccc gttgagtccg ccctaagggt
     2521 cgtggacgat gtacttcgcc agggcatcca gggcatatcc gagttgatca cggagcccgg
     2581 gctgatcaat ctggactttg ccgacgtccg cgctgtgatg tccaatgccg gttcggcgtt
     2641 gatggccatc ggtcgcgcca aaggcgagaa cagggcggct gaggcggcca agtccgctat
     2701 cgccaacccc ctgctggacg tatccatgac gggcgccaag ggtgtgctct tcaacgtcac
     2761 cggagggaac gatctgaccc tcaacgagat caacgatgcc gcggaagtgg tcttcgccgc
     2821 cgccgaccag gaggcgaaca tcatcttcgg ggccgtggtg gacgagagga ttgaggacga
     2881 gatcaggatt accgttatcg ccaccggttt cgacgggacg atcacctctg cctctcccat
     2941 tgaacgggtg gacaggatcc gccctgttcc cagtcagcct gccgtcgcga cacgcccgtt
     3001 ggagaaagtc gagcgcagcg actacgatgt gcccgctttc ctgcgcaggc gacaggagcg
     3061 gtaggattca ataccgacgg caaatggagt ggaggctaga gctggaacgg ttaggcacgt
     3121 tccggctata gcctccacta cggtagatgc agcgaccctg cagggtccgg caattgcgtt
     3181 ttctggcgat gtgatgagcg atcgacttct cgagagtatt gcattctatc cattcgatat
     3241 ctttgcgccc tggccggaac tcacggctgg ggccttcggc cgaagcgggg gagtgagccc
     3301 tgccccttac gattccctga acatctccta cgccgtgcag gacgatccag agagggtgag
     3361 gaccaaccgc gagttgatgg ctcgcgcggt ggggtgggat cccggccgta tcgtggcctg
     3421 cagacaggtg cacggcaccc acgtggtggc cgctacgagt gagatggccg gaggtcccga
     3481 gttgcaggga gtcgacgcgc tggtcaccaa cgagccaggg atactgttga tgttgaagtt
     3541 cgccgactgc gtccccatag tgctatggga ccctgttcgg cgtgttgtcg ccgttgtgca
     3601 tgcagggtgg cgcggcaccg tcgcgagggt gccggcggct gctctgcatt tcatggtgac
     3661 gcagtacggg tcggagccga gcgatgtccg ggcagggatc ggcccatcga taggcccctg
     3721 ttgttatcag gttggacagg aagtcgccac caaggcggag caggtgttca ctgggacagg
     3781 ggtcgtatcg cccgggccgg atggtacccc tcacttcgac ctatggtctg ccaacgccga
     3841 gacgttgatg agatccggag tggcggaaga aaacgtagcc atatccagga tttgcacgcg
     3901 ttgcaggagc gacctgttct tctctcaccg tgcattgggc agcccctcgg ggagatttgc
     3961 gatggtggga gggatacggg atgtgtagcc tgggacagcg gttggaggat gtgcatcgcc
     4021 ggattgccga tgcggctgag cgggccggac gctccccgga ggatatccag ttggtggcag
     4081 tgtccaagac tgtgccggcc gagacggtta tggaggcgta tcggctagga cagaggcatt
     4141 ttggcgagaa cagggtgcag gaatttcgcg agaagcggga cgccctggcc gagctcgggc
     4201 cgatgcccga cgccgtctgg catatgattg gacatctcca gcgaaacaag gccaagccat
     4261 gtgtcgaact gtttgatata attcagtcag tggacagtgt aaagctggcc ggtttgctga
     4321 acgccgatgc gggcgtggca ggcaagcggc tgcggatact gctggaggtg gacttcagcg
     4381 atgagtcgca gcgctcgggt ttctctcccg tggaactgga gagagtggcc ggtgagttgg
     4441 tcttgctgcc ccatgtggag atccagggac tgatgaccgt cgctccgttg ggcctcgacc
     4501 aggaggggac caggaccgtc tttcgccggc ttcgacagtt gagggacagg ctcgctacct
     4561 gctacccggc cgtgagttgg cgccacctat cgatgggaat gagcgatgac tacgttctgg
     4621 ccatcgagga gggggctacg attgtgagag tcggaagggc cattttcgga gagcgctccc
     4681 gcggctgagg tcgcgactgg ccccaggagg aaccgtgctt tttctttacc gtttcgtgga
     4741 tctgctgttc aacattctca tcttcgccat cgtggggcgg gcgctgctct cctggttcaa
     4801 cctgggcccg ggcaacccaa tcgttcggct gctctacgag attacggagc cgatcctggc
     4861 gccgatgcgt cgagtcattc cgatgatcgg catgctggac atttcgccta tcgtggcgat
     4921 cctcttgctc agtttcatgc agaacctgat cttgcaagcg atggtggcgt actagcccat
     4981 ctctgagtgg tcgtagggcg gggtcttgtg ccccgcctcc cgcggtgcgg ccacgaccag
     5041 cacggtgatt acgacctcgc agatggcggg gcacaagacc ccgcccttgt atcttgagaa
     5101 tgcgttagta taggccgaca agttcacgtc ggatgcggca gtccgtcccc gcccttggtc
     5161 ttagaagacc gatacgtagc gttggtcgcc gcgtcctctc atcatgagcc cgaacagttg
     5221 ccagggcata agagcccgca tcatgcaagg atgggccatc tggagcaggg atcgatgaat
     5281 caagaacccc actttgtcgg cattgatgta tccaagactc ggctggatgt ggcggtccgt
//
LOCUS       DATALS010000138         6501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_276535, whole genome shotgun sequence.
ACCESSION   DATALS010000138 DATALS010000000
VERSION     DATALS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276535"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VHS06_02965"
     CDS             complement(<1..283)
                     /locus_tag="VHS06_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013335114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987112.1"
                     /translation="MSKRKRSNSYRPPVLERDRARPRTAPTVSNSVVEGHLTELVSPS
                     TYALADHYRRLGLRERILTLPIMVAMVLAMIWRQVPSVAELIRTSARESL"
     gene            complement(670..2127)
                     /locus_tag="VHS06_02970"
     CDS             complement(670..2127)
                     /locus_tag="VHS06_02970"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX2987113.1"
                     /translation="MQLDYSALGIDMAALMPILVLGVLGMAVLVLGLFVGKEQGGVLA
                     GFSLGGLLVAAVTILPLVGVHRNAFGQMMAVDNFYLFFAMLAIAVTALAVLLSATYLD
                     RTGLAHPEYYGLLIFGAMGMLVMISASHLIMLLIGIELLSLSLYVLAGFARTRLTSEE
                     AALKYFLLGSFSAGLLIYGIALVYGATGTLDYAGIAKAINAQQGLDPMLLIGMGLVLV
                     GFAFKLSFVPFHMWVPDVYEGAPTPVTAYMAVGTKAAVFAAMLRLLGTAFPTLQPEWA
                     PMLAVLAALTMIVGNVAAVVQTNIKRLLGYSSISHAGYVLIAVVAGGALGNDAALFYL
                     VAYTVMNFGAFAVMVAVSGGREERLELSDYAGLAKRSPWLAAAMAVFMLSLSGAPFTP
                     GFLGKLYVFSAAIQGGWLWLALVGVATSLVAFYYYLKVVVVMYMSEPAGEVKPSAATL
                     SMGAVVVVALFFTVQLGVLPSLFFNLSQLPVAMGM"
     gene            complement(2140..3684)
                     /locus_tag="VHS06_02975"
     CDS             complement(2140..3684)
                     /locus_tag="VHS06_02975"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX2987114.1"
                     /translation="MSSTFPFLSVVILLPVAGAIVVPFMGRDRAAQIKLWALLVSLVT
                     LGVAAVMLSAFRTGTAEMQFVEQAPWFPGLGISYHLGVDGISLLLVVLTALLTPVAIG
                     ASWGEMEGRPKEYAVFLLLLEAGMIGAFVALDLILFFLFWEAMLIPMYLIVGICGGER
                     RIYAAFKFFLYTAVGSLLMLLAIIGLAILYKGQTGQLSFDLAQLQTVRVPAQLAMWMF
                     LAFAFAFAIKVPVFPFHAWLPDAYTQAPTAMLVLATMMVKVGAYGFLRFGLTLFPAQM
                     VAVAPVFTVLAVAGVVYASLVAIVQKDIVRLVAYSSVGHMAFIVLGIFSFTAQGISGA
                     TLQMLNHSISTGGLFVLVAMLSRRTKTTTLAQLGGLASRWPVLGALFTLVMFSSAGLP
                     GLNGFVGEFLILLGTFQVQPVAAIFAGTGVVLAAIYLLWMFQRSLHGADCGAGKGFGD
                     LTGGEIVTLVPVVVLVVWIGLFPTSLLSRMEASVGGLVSQVERVSQPSPRVQLTGIPQ
                     LFQLDR"
     gene            complement(3681..5468)
                     /gene="nuoL"
                     /locus_tag="VHS06_02980"
     CDS             complement(3681..5468)
                     /gene="nuoL"
                     /locus_tag="VHS06_02980"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX2987115.1"
                     /translation="MGAFLEMLSLPAAARTVEIPLWSWIVAGKLSVPFSLLLDPLSIL
                     MVLVVTGVGFLIHVYATGYMHGDTDYARFFALMNIFVLAMLMLVMAGNFLLLVVGWGG
                     VGFASYALISFWFQKPANAAAGTKAFVMNSIGDVGMLLATFLIFINFGRVDFTGVFSQ
                     ASQKLAVNSPVATAIVLLLLIGAAAKSAQLPLHTWLPDAMAGPTPVSALIHAATMVTA
                     GVYLIARTHPLYQLSPTAMSVVAIIGAVSALFAATIGLVQPNIKRVLAYSTISQLGYM
                     FLAVGVGAFTAGMFHLVTHAFFKALLFMAAGGVIHALAGEEDLNKMGGLWKKVPSMYI
                     GFLVGALALAGFPMLSGFFSKDEIIFAAFASQQGSPILGAIALAVVALTGFYVFRAFF
                     LCFHGTSRVDPEVAHHIHKPGLSMVAPVGVLTVLAALGGYVNFIGGSGALDRFLAPVF
                     ERYTHPVAMAEGAEAIQEPLMLISVLMGLSGIFVAYLMYVRYPKSAARVGSWYPGLYK
                     LFLNKWYVDELYGFLFLRSGRWLGELLSGPIDWAITDGLVRGTARCVQWCSLGFRRME
                     TGFVRDYALAILLGATVVLFYLIQMGGVR"
     gene            complement(5615..5917)
                     /gene="nuoK"
                     /locus_tag="VHS06_02985"
     CDS             complement(5615..5917)
                     /gene="nuoK"
                     /locus_tag="VHS06_02985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX2987116.1"
                     /translation="MAPHSYYLIVSAILFVLGMTGVLVRKNPIIMFMSIELMLNAANL
                     AFVTFAYRFGAVEGQVVAFFVMTVAAAEVVVGLAIISSIFRIRANVDVDEVSSLKG"
     gene            complement(5936..>6501)
                     /locus_tag="VHS06_02990"
     CDS             complement(5936..>6501)
                     /locus_tag="VHS06_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX2987117.1"
                     /translation="RRGPRGGAEPPLRTGSAEVMELVYFFSVVIVAVLSAIAMLISRN
                     TVHSALFLVVNLFCVALLYLSLTAEFLALVQITVYAGAIMVLFLFVITMLNPSSEETP
                     NKLLSRLPVAVGLAVAFLVGLAGVAMSSVGKAASSTAVMQPAAGNGNLEAIGASLFTQ
                     YLLPFELISFLLLVAMVAAMVLAKRRA"
BASE COUNT         1546 a         2141 c         1872 g          942 t
ORIGIN      
        1 aaagactttc cctggcagag gtgcggatca gttctgccac cgagggcacc tgacgccaga
       61 tcatggccaa gaccatagcc accataatgg gcagagtgag aatgcgctcc ctcaacccca
      121 gacggcgata atggtcggcc aaggcatagg tggagggact gaccaactcg gtcaggtggc
      181 cctccaccac agaattcgag acagtagggg cggttctggg ccgagcccga tcccgctcca
      241 gaacaggcgg acggtaggaa ttgctgcgct ttcgtttgct catacataag cattgcccat
      301 ccgcccacga attgacaact atgctcagcg cttaagttgg gagcgatgca cataggtccg
      361 cccctaccac tcgtcgatct ggctgtgaca gagcactacg cttcgcaggg gctcttctca
      421 tccagggcac atgccgcaac gcgcgcgcag ccaccccctt ttctaggagg gcgggtcgcc
      481 cttgacccgc tcgggccaat cagattacgg gtgatgccat cactctgttc ggtcttcgca
      541 cctgcagcct cctccccgtt gccgctccac aacagaaaac gggatgcatt tctgcacccc
      601 gtccgttcat cctctatacc tacctgatat tggccgacac ctggcccccg ccccctgtcc
      661 cccgcgcagc tacatcccca tcgccactgg cagctgcgac aggttgaaga acaggctcgg
      721 cagcacgccc aactggaccg tgaagaagag cgccacgacc accacggcac ccatggagag
      781 cgtcgcggca gagggcttca cctcccccgc cggctccgac atgtacatca ccaccaccac
      841 cttgaggtag tagtagaagg ccacgaggct cgtggccacc ccaaccaggg ccagccatag
      901 ccaaccgccc tggatggcag cactgaacac atacagcttg cccaggaaac caggggtgaa
      961 gggagcaccg gagagcgaaa gcatgaacac ggccatcgcc gcggcgagcc acgggcttcg
     1021 cttcgccaac ccggcataat cagaaagctc gagcctctcc tcacgtcctc cgctcaccgc
     1081 caccatcacc gcgaacgcgc cgaaattcat cacggtgtag gcgaccaggt agaacagtgc
     1141 agcgtcgttc cccagcgccc cacccgccac aacagcgatc agcacgtagc ctgcatgtga
     1201 tatcgaggag tacccgagga gccgcttgat gttcgtctgc accaccgcag cgacgttccc
     1261 cacgatcatc gtgagagccg ccagaacggc cagcatggga gcccactcgg gctgcagcgt
     1321 ggggaacgca gtccccagga gcctcaacat ggccgcgaaa accgccgcct tagtacctac
     1381 agccatgtag gccgtcacag gcgtaggagc gccctcgtag acgtcgggaa cccacatgtg
     1441 gaaggggacg aaggatagct tgaaggcgaa acccacaagc accagcccca tgccgatcaa
     1501 cagcatcgga tccagcccct gctgggcatt gatcgccttc gcgatccccg cgtagtccag
     1561 cgtgccggtg gcaccgtaga ccagcgcaat accgtagatc agcaggccgg ccgagaagga
     1621 gcccagcagg aagtacttga gtgcggcctc ctcggaggtc agccgcgtcc tggcgaaacc
     1681 ggcaagcacg tacaacgata gcgacagcag ctcgatcccg atcagaagca tgatcagatg
     1741 ggaggcagaa atcatcacca gcatgcccat ggcaccgaag atgagcagcc cgtagtactc
     1801 tggatgggcc agccccgtcc tatccaggta ggtggccgag agcagcaccg ctagagcggt
     1861 gaccgcgatg gccagcatgg cgaagaacag gtagaagttg tccacggcca tcatctggcc
     1921 gaaagcgttc ctgtgaacgc cgacgagggg caggatggta accgcggcaa ccagtagacc
     1981 gccgaggctg aagccggcca gcacgccgcc ctgctccttg ccgacaaaca gcccgagcac
     2041 aagtacggcc attcccagaa cgcccagcac caggatgggc atgagagcag ccatatcgat
     2101 gcccagcgcc gaatagtcca gttgcaaagc tacctcccgc taccgatcca gttggaataa
     2161 ctgggggata cccgtcagtt gaacccgagg cgaaggctgc gaaaccctct cgacctgcga
     2221 aaccaacccg ccgaccgaag cctccatcct gctgagcagg ctcgtcggga agagaccaat
     2281 ccagaccacc agaacgacga cgggcaccag cgtgacgatc tccccgccgg tcaggtcgcc
     2341 gaaccccttc ccggcgccgc aatccgcacc gtggagcgac cgctggaaca tccacagcag
     2401 gtagatcgcc gccagcacca ccccggtgcc ggcgaagatt gccgcgaccg gctgcacctg
     2461 gaatgtcccc agcaggatca ggaactcgcc tacgaagccg ttgagtcccg gtagccctgc
     2521 cgaggagaac atcaccaacg tgaacagcgc gcccaacacc ggccacctcg aggcgagtcc
     2581 gcccagttgg gcgagggtgg tggtcttagt ccgccgagac agcatggcga ccagcacgaa
     2641 caggccgccg gtactgatgg aatggttgag catctgaagc gtggcacccg atatcccctg
     2701 cgcggtgaag gagaatatcc ccaggacgat gaacgccata tggccgacgc ttgagtaggc
     2761 taccagcctc actatgtcct tctggacgat cgccaccagg ctggcataca ccaccccagc
     2821 aaccgccagc accgtgaaca ctggagcgac ggccaccatc tgcgccggga acagggtcag
     2881 gccgaaacga aggaagccgt aggcacccac cttcaccatc atagtcgcca gcaccagcat
     2941 cgccgtggga gcctgcgtgt aagcgtcggg caaccaggca tggaagggga acaccggcac
     3001 tttgatggca aaggcgaagg cgaaggccag gaacatccac atggccagct gagccggcac
     3061 ccgcaccgtc tgcagctgcg cgagatcgaa gctgagttgc ccggtctggc ccttgtacag
     3121 gatagccagg ccgatgatgg ccagcagcat cagcaagctg ccgaccgccg tatacaggaa
     3181 gaacttgaag gcagcgtaga tgcgcctctc gccgccacag atcccgacga tcaggtacat
     3241 cgggatcagc atcgcctccc agaacaggaa gaagaggatc aggtccagcg ccacgaacgc
     3301 gccgatcatc cccgcctcaa gcagcagcag gaagaccgca tactccttgg gccgtccctc
     3361 catctctccc caggaggcac cgatcgccac cggcgtcagc agcgccgtga gcacgaccag
     3421 caacaggctg atcccgtcta cccccaggtg gtagctaatc cccagcccgg ggaaccaggg
     3481 cgcctgctcg acaaactgca tctcggcagt gccggtccgg aaggccgaga gcatcacagc
     3541 cgcgacgcca agagtcacca acgagaccag cagcgcccac aacttgatct gcgcggctcg
     3601 gtcgcgaccc ataaagggaa ctacgatggc ccccgcgacc ggtagcagga tcactacgct
     3661 caggaagggg aatgtagagc tcatcgaacc cctcccatct ggatcagata gaacagcacc
     3721 accgtcgccc ccagcagaat tgccagcgca taatcccgga cgaaaccggt ctccatccgt
     3781 ctgaagccca gcgagcacca ctgcacgcaa cgcgcggtgc cacgtacaag accgtcggtg
     3841 attgcccagt caatcggacc ggagagcaac tcacccagcc acctgcctga ccgcaggaac
     3901 aggaagccgt acaactcgtc aacgtaccac ttgttcagga acagcttgta cagccccggg
     3961 taccagcttc cgaccctagc cgcggacttc gggtagcgga cgtacatcag gtaagctacg
     4021 aagataccgg agagtcccat cagcaccgag atcagcatca acggctcttg gatcgcctct
     4081 gcgccctcgg ccatcgccac cgggtgggtg tatcgttcga acaccggggc caggaaacga
     4141 tcgagagcac cggacccgcc gatgaagttg acgtagcctc ccaacgctgc caggacggtc
     4201 aacacaccga caggggcgac catcgaaagg ccgggcttgt gaatgtgatg ggccacctct
     4261 ggatccaccc gactcgtacc gtggaagcag aggaagaagg ccctgaacac gtagaacccg
     4321 gtgagcgcca ccacagccag cgcgatcgcc cccaggatcg gactgccctg ctggctggca
     4381 aaggcggcga atatgatctc gtccttgctg aagaagcctg agagcatcgg gaagcccgcc
     4441 aaggccaggg caccgaccag gaagccgatg tacatggagg gaaccttctt ccacagcccg
     4501 cccatcttgt tgagatcctc ttcaccggcc agcgcgtgga tgaccccgcc ggcggccatg
     4561 aacaggaggg ccttgaagaa ggcatgggtg accaggtgaa acataccggc tgtaaaggcc
     4621 ccgacaccga cggccaggaa catgtaacca agctggctga tggtggagta ggccagtact
     4681 cgcttgatat tcggctgaac cagtccgatg gtcgcggcga atagggcgct gacggcgccg
     4741 atgatagcca ctaccgacat agccgtcgga ctcaactggt agagcgggtg tgtcctggca
     4801 atcaggtaga cgccggcggt aaccattgtt gccgcgtgga tcaacgcgct caccggcgtc
     4861 gggcctgcca tggcgtcggg aagccaggtg tgcagcggta gctgggcaga cttagcagca
     4921 gcgccgatca gcagcaacag cacgatggcc gtggccactg gactattcac agccagcttc
     4981 tgagacgcct gcgagaacac tccggtgaag tccactcgcc cgaagttgat aaagatcagg
     5041 aaggtcgcca gcaacatgcc gacgtctccg atggagttca tcacgaaggc ttttgtcccg
     5101 gccgccgcgt tggccggctt ctggaaccag aaggagatca gggcgtaaga agcgaagccg
     5161 acgccgcccc atcccaccac cagcagcagg aagttgccgg ccatcaccag catcagcatc
     5221 gccaggacga agatgttcat cagggcgaag aagcgggcgt agtcggtgtc gccgtgcatg
     5281 tagccggtgg catacacgtg gatcaggaag cccactccgg tgaccaccaa caccattagt
     5341 atggaaagcg ggtcaagcag caaggagaag ggcaccgaga gcttgccggc aacgatccag
     5401 ctccagaggg gaatctccac cgtccgggcc gcggccggca aactgagcat ttcgaggaaa
     5461 gcgcccaccc cccacgacga aagaaagccc taccgtcccg ctggcaacga cgccgcagga
     5521 tcggcggcta atcagccgtc cgaccaggga gcaatagagg aaaccgagcg ctggaaatgc
     5581 aagtatcagc caggcgttgc tcaacaagag gaaatcaccc cttcagtgaa ctgacctcgt
     5641 cgacgtcgac gttggcacgg atccggaaga tggaggagat gatcgccagt cccaccacca
     5701 cctctgccgc cgccaccgtc atcacgaaga aggctaccac ctggccctcc accgctccga
     5761 agcggtaggc gaaggtgacg aaggccagat tggccgcgtt cagcatcaac tcgatggaca
     5821 tgaacatgat tattggattc ttcctcacca gaaccccggt catcccgagg acgaagagga
     5881 ttgcactgac tatcagatag taggaatggg gagcgatcat agaactatac ccaccttacg
     5941 ctcgccgctt tgcaagtacc atagccgcga ccatcgcaac cagcaacagg aaagatatca
     6001 actcgaacgg gagcaggtac tgcgtgaaca gactggcacc gatcgcctcc aggttcccgt
     6061 ttcccgccgc aggctgcatc accgccgtcg aagacgccgc cttaccgacc gaagacatag
     6121 ccacaccggc cagccccacc aggaaggcca cagccaaccc caccgccacc ggcaaccggc
     6181 tgagcagctt gttaggggtc tcctcgctgg atggattgag catcgtgatg acgaaaagga
     6241 agagcaccat gatggcgccc gcgtacaccg tgatctgcac caacgccagg aactcggccg
     6301 tgaggctcag gtagagcagg gcgacgcaga acaggttcac caccaggaac agcgcgctat
     6361 gaacggtgtt ccggctaatc agcatggcga tcgccgagag caccgccacg attacgacag
     6421 agaagaagta gacgagttcc attacttcag cacttcctgt acgtagggga ggctccgcac
     6481 ccccacgggg gcctcgccgt g
//
LOCUS       DATALS010000139         5426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_193391, whole genome shotgun sequence.
ACCESSION   DATALS010000139 DATALS010000000
VERSION     DATALS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193391"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1417
                     /locus_tag="VHS06_02995"
     CDS             23..1417
                     /locus_tag="VHS06_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2987118.1"
                     /translation="MRDFFSDLAFAGSCLAMGVILALLLSKLIAKRIQYITLSTARIG
                     AGDLSQRLNVTVHDEIAKLAAQFNVMVEQLQQAMDSEKAVAERYQRLLENIADGYVIL
                     QNGEIVYSNRQMGTMLGYDVASLVGIKVASLVSPEFAKKAENHLNSRLRGEELPSNSE
                     WELRTADGRYLPIEINSHTIEYLGRPALASTVRDVTQRRQMEAQRARLIREEAEGKAL
                     RDLLHQKNRLVSTISHELRTPLCLVMGFSEILYTRNLNQEQMHSVCREIHSGSLRLKR
                     IVDEIVQFAEVQGTSAPIEPTIVDLVELTRDCVEEMAAEEPRHIFKVQIPEQLERVRA
                     AIPPGRFRDALGHLLSNAARFSPSGSEVQVSLGVNKENATVTVVDQGVGIAPEELGRV
                     FEPFFRTERSHRRAVPGTGLGLAITKRLIELWGGQITAHSNTGHGSRFSFTVPLVDSH
                     PSVETASGNTADCG"
     gene            1540..1725
                     /locus_tag="VHS06_03000"
     CDS             1540..1725
                     /locus_tag="VHS06_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987119.1"
                     /translation="MDAEEMQTPQQGESRPEPSSDSSAWSEEAKGWYEELKTQEDDLA
                     PWLSVEWTGEEMKVNRG"
     gene            1883..2164
                     /gene="rpsT"
                     /locus_tag="VHS06_03005"
     CDS             1883..2164
                     /gene="rpsT"
                     /locus_tag="VHS06_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX2987120.1"
                     /translation="MANTKSAIKMIRVALRRHNRNIPIRSELKTLVKAARADIDAGQI
                     EVAQGDVRKAASALDKAANKGTIHRNAAARRKSRLMKRLAKAEKATAAS"
     gene            2456..3112
                     /locus_tag="VHS06_03010"
     CDS             2456..3112
                     /locus_tag="VHS06_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2987121.1"
                     /translation="MDAPKRMPRYDIRNDGIGPYAVFYCDKCEREYRSKPNVGNTIAK
                     DIGRDAVGGFFRGIPLIGHAVADNVVGEDPRYTNRLTDEQLAEAWAQVQENFRQCPTC
                     LLTVCLSDFDVQSGYCQDDSPRREQIAEARAEQAAGVVKGIASAFGFGEAIKVASEAA
                     KAAASESARCPNDGTTAPAGTKFCPRCGTAMIQPTKSVCPSCGKEVAAGTRFCPECGS
                     "
     gene            3353..3982
                     /gene="lexA"
                     /locus_tag="VHS06_03015"
     CDS             3353..3982
                     /gene="lexA"
                     /locus_tag="VHS06_03015"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX2987122.1"
                     /translation="MRQLSQRQRRILDFIARFSQRHGYPPSIREIGQEVGISSTSVVD
                     YNLKVLERQGYIRRDREVSRGLELTDVSSARRPDGAVRVPIVGRIAAGEPIEAVQGHQ
                     DEVEISEKLCAEGCFALQVKGKSMIEDLIDDGDLVVIRPQESADDGDIVVALLTEGPT
                     LEGRATLKRLYREKDRIRLQPANSSMEPIYVSGEELRVQGKVVAVIRQL"
     gene            complement(3969..5147)
                     /gene="holA"
                     /locus_tag="VHS06_03020"
     CDS             complement(3969..5147)
                     /gene="holA"
                     /locus_tag="VHS06_03020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX2987123.1"
                     /translation="MVWSDMAAPPGGVAADLLRFIIDMGVGVHNDRAMLDGRGRVQYD
                     SRTMVETRANWIYLFHGEDDLSSREAVQGLVARMKDSPVWECNISTFDGEKLALPDLT
                     AVTQTVPFLAEKRLVVVSNFLSRLSDSGRESGRSEDAKPERAARGSKKAHLEAVLGLL
                     PLVPSFCRLVFVEDRLVPEKDPVLQAIRGLGGYVKAHRLDEGGLGSWIRARATKIGCK
                     FSTGAVEELGSAVGPNARLLHSEMLKLATYCQDRAVEIEDVRLLVADARKANVFAMVD
                     ALGQRQPEAAVRELRKLLSEGDHPLRIVAMVVRQFRLLIQVKELSEAGASPDEIAQKA
                     GAPYRGVMGLQRQARNFSFTQLERAYRKLLDYDVQVKTGACDPEAALEFLMADLLRAA
                     "
BASE COUNT         1177 a         1657 c         1618 g          974 t
ORIGIN      
        1 gtagttggtg ttcggccctc agatgaggga tttcttctca gatctcgcat tcgcgggttc
       61 ctgtctggca atgggcgtca tactggcgct gctgctgagc aagctaatag ccaaacggat
      121 tcaatacatc accttaagta cggcaaggat cggggctggg gatctctccc agaggctgaa
      181 cgtcaccgtg cacgatgaga tagccaaact ggctgctcag ttcaatgtca tggtggaaca
      241 gttgcagcaa gcaatggaca gtgagaaggc agtagctgag cgataccaga gactgctgga
      301 aaacatcgcc gatggctacg tcatcctcca gaacggggag atcgtgtatt ccaaccggca
      361 gatgggtacc atgctgggct acgacgtggc ctcgctagtc ggtatcaagg tggcgagcct
      421 cgtatcccct gagttcgcca aaaaagcaga gaatcatctc aacagccgcc tgcgtgggga
      481 ggagttgccg agcaacagcg agtgggagtt gcggaccgcc gatggccgct acctgccgat
      541 tgaaatcaac tcccacacca tcgagtacct ggggcggccg gcgctcgcct caaccgtccg
      601 agacgtgacg cagcgtcgcc agatggaagc acagcgggcc cggctgatca gggaagaggc
      661 cgagggaaaa gcgctgcggg atctgctgca ccagaagaac cggctggtca gcactatctc
      721 ccatgaactg cgaaccccac tatgtctcgt gatgggcttc agtgagatcc tctacacacg
      781 caatctgaac caggaacaga tgcactcggt ttgccgcgag atccacagtg gatccctgcg
      841 tctcaaacgg atcgtggatg agatagtcca gttcgccgag gtgcagggaa cttctgcccc
      901 gatagagcca accatagtag acctcgtgga actgactcga gactgcgtgg aagagatggc
      961 ggcggaagag ccgcgccaca tcttcaaggt ccaaatccct gagcaactcg agagggtgcg
     1021 ggctgccatc cctcccgggc gtttccgcga cgccctgggc cacctgttgt ccaacgccgc
     1081 aaggttctcc cccagcggca gcgaggtgca ggtttccctt ggagtgaata aggagaacgc
     1141 aaccgtgact gttgtcgatc agggggtggg gatagcaccg gaagaacttg gccgggtgtt
     1201 cgagccattc ttccgcaccg agaggagcca caggcgagca gttcctggca ccggtctcgg
     1261 actcgccatc accaaacggc tgattgagct gtggggaggg cagatcacag cccatagcaa
     1321 tacagggcat ggaagccgtt tcagcttcac cgtcccgctg gtcgactccc atccgtcggt
     1381 cgagaccgct tccggcaaca ccgccgactg cggttgatcc cctttccggc gtgggctatc
     1441 cttgtagaag cttggcagaa aacgccgtag caccagtccg tccaacacag ccacggactc
     1501 gttccgttca ctaaccccag aacttgatgg tggagagata tggacgcaga ggagatgcag
     1561 accccacaac aaggggaaag ccggccggag ccctcttcag acagttctgc ctggagcgaa
     1621 gaggcgaagg ggtggtacga ggaattgaag acacaggagg acgacctcgc cccgtggctc
     1681 agcgtggagt ggacaggcga ggagatgaag gtcaatcgcg gctgaagtct taattggaca
     1741 aatccggcag actgctgtat tattctgctt tgctacaggg aatgtctggt ccaacgtggg
     1801 tcaccgtcgt cctgagaggt tagtggtgat cgcgctgggc cggtttttgt gaagtttaga
     1861 accccgcggg agaggtgaca ggttggccaa tactaagtcc gccatcaaga tgatccgggt
     1921 cgccctcagg cgacacaatc gcaacatacc cattcgctcg gagctgaaga ccctggtgaa
     1981 ggccgcgcgc gcagacatcg atgcaggcca gatcgaagta gcccagggcg acgtgaggaa
     2041 ggctgccagc gcgctggata aggccgcgaa caagggtacg attcatcgga acgccgcagc
     2101 aaggcgaaag tcccgactga tgaagaggct cgccaaggct gagaaggcaa cggcggcgag
     2161 ctagaagggc aagtcccact tcacagcgcc cgtacaatac tcaaacctct gacctcgggt
     2221 cgttcggggt agtcgttgtc gcccaacaca gggctgatcc agatggctac ccctcttttc
     2281 tgcccatcgg cccccctcgc ggaaccaccc tgagaacacc acccaccaat ccgtccaatt
     2341 gcgacaccct caccacttgc tgatacccga ctgatagctt ctgcacgcta tactgtgagc
     2401 acttctccga agaatgatca tagcccctgc tgagaggggg acgatggagg ctaggatgga
     2461 cgccccgaag cgcatgcccc gctacgatat ccgcaacgac ggcattggac cctacgcggt
     2521 cttctactgc gacaagtgcg aaagggagta ccgcagcaag ccaaacgtgg ggaacaccat
     2581 agccaaggac atcggtaggg atgcggtagg cgggttcttc cgtggaattc cgctcatcgg
     2641 tcacgcggtg gccgacaatg tggtaggtga agatcctcgg tacacaaacc ggctcaccga
     2701 cgagcaactg gcagaagcat gggcgcaggt gcaggagaac ttccgccaat gcccgacctg
     2761 tctgctgacg gtctgtctgt ccgacttcga cgtccagagc ggatactgcc aggatgatag
     2821 ccccaggcga gagcagatcg cggaggcacg agccgagcag gccgcgggcg tagtgaaggg
     2881 catcgccagc gccttcggtt tcggagaggc gatcaaggtt gcgagtgagg ccgcgaaagc
     2941 tgcagcttca gaatcggctc gctgtcccaa cgatgggacc acggccccag caggcaccaa
     3001 gttctgtccg cgatgcggga ctgccatgat ccaacccacg aaatccgttt gccccagctg
     3061 cggcaaagag gtggccgcgg gaaccaggtt ctgcccggag tgcggaagtt agatcgagca
     3121 gcctgctcca gttcccagcg tctgccccag ctgcggtgcc gacgcaagag gcgccaagtt
     3181 ttgctcgcag tgcggctcca agatcggctg aaatcgatcc tattggtggc cggcaggctg
     3241 ccggccacca atcctccatc aatccaagcc tcgacttgac actacggtta tgaggctata
     3301 gaatgcccta gaacgaacgt tctagggttc tttcgattcg tggaggacag ccatgcgcca
     3361 actatcccag aggcagcggc gtatcctgga tttcatcgcc cgcttttcgc agcgccacgg
     3421 ctacccccct tcaatccggg aaatcggcca ggaggtcggt atctcctcca cttctgtagt
     3481 cgactacaac ctcaaggtac tggagcgcca gggctacatc cgcagagacc gcgaagtatc
     3541 cagggggctg gaactgaccg acgtcagcag tgcccggagg ccggatgggg cggtccgcgt
     3601 ccccatcgtc ggccgcatcg ccgcgggcga gccgatagag gcagttcagg gccatcagga
     3661 cgaggtagag atctcagaga agctgtgcgc cgagggctgt ttcgcactgc aggtcaaggg
     3721 aaagtcgatg attgaggacc tgatcgacga cggtgacctg gttgtgattc gcccccagga
     3781 gagcgccgac gacggggaca tcgtcgtcgc actgctgacc gagggaccaa ccctcgaggg
     3841 tcgagcaacc ctgaagcggc tttaccgcga gaaggatcgg atccggctgc agccggccaa
     3901 ctcctccatg gagccgatct acgtgtctgg tgaagagttg agggtacagg gcaaggtggt
     3961 ggccgtcatc aggcagctct gagcagatcc gccatcagga actccagggc agcctccggg
     4021 tcgcatgcgc cggtcttgac ctgaacgtcg tagtccagaa gtttccggta tgcccgctcc
     4081 aactgggtga agctgaagtt gcgagcctgc cgctgcaatc ccatcacgcc tcgatagggg
     4141 gcacccgcct tctgcgcaat ctcgtcgggc gaagctccag cttcggagag ctccttcacc
     4201 tgtatcagca gcctgaactg ccgcaccacc atggccacaa tccgcagcgg gtgatcgccc
     4261 tctgagagca acttccgcaa ctcgcgaacc gccgcctcag gttgtctttg gccaagggca
     4321 tccaccatgg caaagacgtt cgccttcctc gcatctgcca ccagcaggcg aacgtcctcg
     4381 atttccacgg cgcgatcctg gcaataggtg gccagtttca gcatttcgga gtggagcagc
     4441 ctggcgttgg ggccgaccgc gcttcccaac tcctccacgg ccccggtaga gaacttgcag
     4501 ccgatcttgg tggcacgagc cctgatccag ctgccgagtc caccctcgtc cagccgatga
     4561 gccttcacat atccgccgag accacgtatc gcctggagga ccgggtcctt ctccggtacc
     4621 agccgatcct cgacaaacac caacctacag aatgagggca ctagaggaag cagtcccagt
     4681 accgcctcga gatgggcctt cttgctaccc ctggcggcgc gctccggttt cgcgtcctcg
     4741 gacctgccgg attccctccc tgagtcggag aggcgcgaca ggaagttgga caccacgacc
     4801 aacctctttt ccgccaggaa gggaacggtc tgagtgacgg ccgtcagatc agggagtgcc
     4861 agcttctccc catcgaaggt ggagatattg cactcccaaa cgggggaatc cttcatccgt
     4921 gcgaccaatc cctgcaccgc ctcgcgagaa gagagatcgt cctcaccgtg gaataggtag
     4981 atccagttgg cgcgtgtttc aaccatagtg cgggaatcat actgcaccct gccccttccg
     5041 tcgagcatcg cgcgatcgtt gtgtacaccg acacccatat ctataatgaa cctcagcagg
     5101 tccgccgcga ctcctcccgg aggtgccgcc atgtccgacc agaccacgcc acccccccct
     5161 ggacagggag gaaacggctg cccagccaag tcgagatgaa tcgcccgccc ttcccgggca
     5221 gccttcgctt gcggatggct cgaccaggca gcggatgatg gttggggcgg ctttgctgac
     5281 aatgacgtcc tcagtattgc tgttctgcat cggtatcatc acgcatacgg ggcagggcga
     5341 agccagcatc gtagccgcat cctcgccatc gcccatatcg gcactcaatc caaccgccat
     5401 cccaatgccc tccgccacat ccacgc
//
LOCUS       DATALS010000140         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_13421,
            whole genome shotgun sequence.
ACCESSION   DATALS010000140 DATALS010000000
VERSION     DATALS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13421"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VHS06_03025"
     CDS             <1..267
                     /locus_tag="VHS06_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2987124.1"
                     /translation="VPMVSHVDHTEHDVQVVVTEQGLADLRGLSPKQRALQIIEKCAH
                     PDYRPALREYYRRALELSPGKQTPHLLDESLNWHLRFMKTGKMA"
     gene            complement(335..1609)
                     /locus_tag="VHS06_03030"
     CDS             complement(335..1609)
                     /locus_tag="VHS06_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2987125.1"
                     /translation="MAQESRIHATSLQSKIMSAEEAAALIPPGAKIGMSGFTGSGYPK
                     AVPQALANRIEAANSAGEKFRVSIWTGASTAPELDGALAKVDGVELRLPYQSDPACRE
                     RINSGRMEYIDIHLSHVAQYAWFGFLGKLDFAVIEVAGILEDGRLIPSSSVGNNKTWI
                     ELADKVILEVNSWQSAELEGMHDVYYGTRRPPYRQPIPITSTGDRIGTPYLTCPPEKV
                     VAVVETHAPDRNSPFSAPDENSQRIAGHILDFLDNEVKQGRLPENLLPLQSGVGNIAN
                     AVLSGLLEAPFENMTSYTEVIQDGMVELMKSGRLTTASATAFSLSTDMTHYLNAHMGE
                     YKKRIILRPQEISNHPEVIRRLGCLAMNGMIEADIYGNVNSTHVMTAWNLPDPNWTTD
                     REEYWWSIANADGSDRPAYTQLKQARQSGQLP"
     gene            complement(1664..2080)
                     /gene="mscL"
                     /locus_tag="VHS06_03035"
     CDS             complement(1664..2080)
                     /gene="mscL"
                     /locus_tag="VHS06_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX2987126.1"
                     /translation="MSGFRKFILRGNVVDLAVAVVIGAAFGAVVQALVKDIITPIIGA
                     FGGVADFSAWFFTVNGSKFLIGDFINTLLSFLVIAFVVYYFVVVPVQKLMDRYKGEPA
                     VVPASTRECPYCLSKIPARASKCAFCTADVPPESKV"
     gene            2336..>3386
                     /gene="argS"
                     /locus_tag="VHS06_03040"
     CDS             2336..>3386
                     /gene="argS"
                     /locus_tag="VHS06_03040"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX2987127.1"
                     /translation="MTTVRGQLRDLLADAVQRAQQQGVLPAVAVPEITVENPQNPEHG
                     DYASNIALRMARTARMDPMSIALALANQMSSVGFLSSVDVARPGFLNFRLSPAWLASQ
                     VEEILAQGDRFGAISLGNGEKVQIEYVSANPTGPLHVGAGRGAALGDSLARILSLAGY
                     KVEREYYVNDAGARMEAFYSTLYARYLQQFDVPAEVPADGYAGEYMVELAGEIAQERG
                     REFLDMPREEALPKLGRLGTDKMVGRIRADLARMNVHFDNWFSEQSLYDNSQVTKSIG
                     ILQKKGYVAEREGAVWFVSSALGEEKDNVLVRTNGAPTYFASDVAYHYDKFMLRGFDK
                     VIDIWGADHQGHVPRM"
BASE COUNT          668 a         1044 c         1024 g          650 t
ORIGIN      
        1 gtgccgatgg tctcccatgt cgatcacacg gagcacgatg tacaggtggt ggtcacggag
       61 cagggattgg ccgatcttcg cggcctatcg ccgaagcagc gggcgttgca gatcatagag
      121 aagtgcgccc acccggatta ccggccggca ctgcgggagt actaccgacg tgctctggag
      181 ctatccccgg gcaagcagac cccccacctg ctggacgagt cgctcaactg gcacctgcgc
      241 ttcatgaaga ccggcaaaat ggcttagtag cgcagacaca aagagacggg gcggagaaat
      301 ccgccccgtc tctttgtgtc tgcctcgaag gcgactacgg cagctggccg ctctgtcgag
      361 cctgcttcaa ctgagtgtag gccgggcgat cggagccgtc ggcgttggcg atggaccacc
      421 agtactcctc tcggtcggtc gtccagttgg gatcgggcag gttccaggcg gtcatcacgt
      481 gggtggagtt cacgttcccg tagatgtccg cctcaatcat tccgttcatc gccaggcatc
      541 ccagccgccg gattacctcc gggtgattgc tgatctcctg gggacggagg atgattctct
      601 tcttatattc ccccatgtgg gcgttcagat agtgggtcat atccgtgctg agggagaagg
      661 ccgtcgcgga tgcagtcgtc aacctccccg acttcatcaa ctcgaccatg ccatcctgaa
      721 tgacctctgt gtaagaggtc atgttctcga agggtgcttc cagcagcccc gataggacgg
      781 cgttcgcgat attgccgacc cccgactgaa gcggcagaag attctcaggc agccgcccct
      841 gcttcacctc gttgtccagg aagtcgagaa tgtggcccgc aattcgctgc gagttctcgt
      901 ccggcgccga gaaaggactg ttgcggtctg gagcgtgcgt ctcgacgacg gcgacgacct
      961 tctccggcgg acacgtgagg taaggagtcc cgatgcgatc gccggtgctg gttatcggga
     1021 tgggctgccg atagggtggg cggcgcgtcc cgtagtagac gtcgtgcatt ccctccagct
     1081 cggcgctctg ccaggagttg acctccagga tcaccttgtc ggcgagctcg atccaggtct
     1141 tgttgttgcc cacggacgag gaggggatca acctaccatc ctcgagtatt ccggcaacct
     1201 cgatgacagc gaaatccagc ttgccgagga agccgaacca ggcgtactga gcaacgtggc
     1261 tgagatgtat gtcgatgtac tccatccgcc ctgagttgat ccgctcacgg caggcggggt
     1321 ccgactgata ggggagtcgc aactcgacgc cgtctacctt cgccaacgcg ccgtcgagtt
     1381 cgggtgcggt agaggcaccc gtccagatgc ttaccctaaa cttctcgccc gcgctgttag
     1441 ccgcctcgat gcggttggcc aacgcctgag gtactgcctt cgggtaaccg gagccggtga
     1501 acccgctcat gccgatcttg gcgccgggcg ggatgagggc cgcggcctcc tccgcagaca
     1561 tgatcttgct ctgtaacgag gtggcgtgga tccgggattc ctgagccatt gcgtctctac
     1621 tccgttgtag gaatgagcgc ttgccacgag ggaagcgctg tacttatacc ttggactccg
     1681 gcggaacatc cgcggtgcag aatgcgcact ttgacgcccg cgcagggatc ttgctcagac
     1741 aatacgggca ctcccgtgta ctcgccggca cgaccgccgg ctcacccttg tacctgtcca
     1801 tcagcttctg taccggcact accacgaagt agtagacgac aaaagcgatc acaaggaacg
     1861 acagaagggt attgatgaag tctccaataa gaaacttgga gccgttgacg gtaaagaacc
     1921 aggccgagaa gtcggcgacg ccgccgaagg ccccaataat cggcgtgatg atgtctttga
     1981 ccagcgcctg aaccacagca ccgaatgcag cgccgatcac cacggccacg gctagatcga
     2041 ccacgttccc gcgcagtatg aacttcctga agccgctcat ctctcaaacc cctcatccga
     2101 tcgcacgaga ctcaacacgg tcgggcagtc agtcgctatt atcgagtatg ctgacccggt
     2161 tgacaacgcc atctccgaat ccaagagctc cgtacatcac gtctcaactg cgttccgcag
     2221 gcgatagcgg gccgctgcat cgggtaattc gtttgcctgc ccccacgccc tgggctataa
     2281 tgggttatac gtctggcctt cgggtcagac gagatcgttt gtaggagcca taaacgtgac
     2341 caccgtgcga ggccagctac gcgacctcct ggcggatgcc gtccagcgcg cgcagcagca
     2401 gggcgtgctc cccgccgtgg cagtacctga aatcacagtc gaaaacccac agaaccccga
     2461 gcacggggac tacgccagca acatcgccct tcgcatggcc cgcacggccc gcatggaccc
     2521 catgtcaatc gccctcgctc tggcgaatca gatgtcctcc gtgggcttcc tatcgtcagt
     2581 cgacgtggca agaccgggct tcctgaactt ccggctgtcc ccggcatggc tggcatccca
     2641 ggtcgaggag atccttgccc agggagaccg cttcggcgcg atatcgctgg gaaacggcga
     2701 gaaggttcag atcgagtacg tcagcgccaa ccccacgggc cccctccacg tgggtgcggg
     2761 ccgcggcgcc gcgctgggag acagcctggc caggatactc tctctagccg gctacaaggt
     2821 cgagcgagag tattacgtaa atgacgcggg tgcccggatg gaggcgttct actccaccct
     2881 ctacgcccgc tatcttcagc agtttgacgt ccccgcagag gtgccggccg atggctacgc
     2941 gggcgaatac atggtggaac ttgccggcga gatcgcccag gagcgaggtc gcgagttcct
     3001 cgatatgccg cgggaagaag cgctcccgaa gctgggtcgt ctgggcacag acaagatggt
     3061 cgggaggatc agggccgacc tggcgcggat gaacgtccac ttcgacaact ggttctccga
     3121 gcaatcgctg tacgacaaca gccaggtgac caagtcgatc ggtatcctcc aaaagaaggg
     3181 ctacgtcgcg gagcgagagg gcgcggtctg gttcgtttcg tcggctctgg gcgaagagaa
     3241 ggacaatgtt ctcgtccgca ctaacggtgc acccacctac ttcgcatccg acgtggctta
     3301 ccattatgac aagttcatgc tgcgtggctt cgacaaggtg atcgacatct ggggtgccga
     3361 ccatcagggc cacgtgcctc ggatga
//
LOCUS       DATALS010000141         6375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_147624, whole genome shotgun sequence.
ACCESSION   DATALS010000141 DATALS010000000
VERSION     DATALS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147624"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VHS06_03045"
     CDS             <1..403
                     /locus_tag="VHS06_03045"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX2987128.1"
                     /translation="AAGTSTVMLGNLLAGTTEAPGLTIRRGGRPYKISRGMASAEAAL
                     ARAEREHPDRGWAEWEDAIEDVVPEGVEAVVPYRGDVGEIVFQLVGGLRSGMSYCNAR
                     TLEELRQNATFVRITEAGRKESGPHDIEIMG"
     gene            415..555
                     /locus_tag="VHS06_03050"
     CDS             415..555
                     /locus_tag="VHS06_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987129.1"
                     /translation="MSPESYAGSVRAPDFPAGLDWLNVDHPLSLAELRGKLVLLDFWT
                     YG"
     gene            532..1902
                     /locus_tag="VHS06_03055"
     CDS             532..1902
                     /locus_tag="VHS06_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015150006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:553..555,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HEX2987130.1"
                     /translation="MDFWTYGUINCIHVLPQLRKLEDAFPNELVVVGVHSPKFQGERP
                     TPNLRQAVLRMGIQHPVINDPGMRLWSEYSVRAWPTVMLIDPEGRVIGNHEGEFRFEE
                     FRPLIEAMISEFEAKGNLNRQPLTLRPEEPPETTLLFPGKLLAHEPSGRLFVADSGHD
                     RILVCGLDGSVRQVIGGQKGFRDGDIAGAAFDMPLGMALEGDRLYVADCWNHALRRID
                     LDSGRVVTVAGTGEQARRVKEGNGPESPLNSPWDLAIHQGTVYIAMAGSHHIWSYDPR
                     SGQIGRYAGSGREALVDGPLVRATFAQPSGLSTDGRAMYVADSESSAVRTVDLPGAAG
                     EVRTLVGQGLFEFGDVDGSGDMVRLQHNLGVCFDPQEGVVFVADSYNHKIKQLEPRTR
                     RVDTILGDGEAALRDGIGEAASFWEPGGVSVAGGRLYVADTNNHAVRVTDLATRQVTT
                     LEITGA"
     gene            2229..3587
                     /locus_tag="VHS06_03060"
     CDS             2229..3587
                     /locus_tag="VHS06_03060"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEX2987131.1"
                     /translation="MKRCRLPLVLALGAVLALMVVGCGAVQRGSAPGAIAALWSASST
                     AGGNADSSSATDTKATAADKNASDSSKSTEKKSAASNKPNFLSTNGTRIVDADGNDVK
                     ITGVNWFGMETGTFAPHGIWARNWEDMLDQIASLGFNTIRLPYSNQLFDPESKVGEGV
                     DIKLNPDMEGLSGMQVMDKIVEGAGKRGIKVILDQHRPDVNAQSKLWYTDKYSEERWL
                     SDWVMLAKRYKGNDTVIGADLHNEPAGNATWGSGDPKTDWRMAAEKAGNAIGEVNPDW
                     LIIVEGIEKTNNDKDWYWMGGNLSDAAKYPIKLKVPNKLVYAPHDYGPEVYEQGWFKD
                     TSFPANLPAVWDAHWGFIAKDGIAPLLLGEFGGKSVGEDAGGTWQRSLMDYIKQNGLN
                     YTYWSFNANSGDTGGILTDDWKTVNEAKLSMLSGYKDKMMRVMNPSKIDITAAPSGKS
                     GK"
     gene            complement(3638..4099)
                     /locus_tag="VHS06_03065"
     CDS             complement(3638..4099)
                     /locus_tag="VHS06_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987132.1"
                     /translation="MKKIGVDLRRLAVPIALVALFSGLALYHVTSALAAAAASPVCVN
                     ISASVTPDPVEKLDRVTVASRFSALGAVAEPLQARLDVLSVEGGNSVLTMKQSGFRLP
                     EMGSRAIYWEWRVPDSVPEGQYALRLTVVGADSGRLYGEVDNAATLAVVRR"
     gene            complement(4137..4862)
                     /locus_tag="VHS06_03070"
     CDS             complement(4137..4862)
                     /locus_tag="VHS06_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987133.1"
                     /translation="MGWKGERQLRAIWFALFATTGLIVGGFVIGYGVALLGAPAPSGA
                     PAVVPLPTLAPVLPGTDHATGVDGDGGASGSTASAAATPVSDASADVTTVAGVGGQPR
                     LVTTPTVPVSESRDTSTDFSLPAEHTIGNGLVVSLESIDVNTAQGGVELLLELRNGSQ
                     RGVTFSFLPESDLSVTDGQGQSYDMRWSEYVGMVSVAPGEKVQLVRAFFAGLPSNGTH
                     HLLVRVGHAPQLPESSWWVPVSR"
     gene            complement(4875..>6375)
                     /locus_tag="VHS06_03075"
     CDS             complement(4875..>6375)
                     /locus_tag="VHS06_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987134.1"
                     /translation="PFLIVRAGSVKRGAEVVVGGQSFQPYQELSITIKPSGDGKPLGL
                     GTVTADKNGVVADSSLGLPQELGTGDYTVVATPTKGGASVEASLRVISDQTWTKLTAD
                     TAKTGGAVGYDGGGFAPGEIVSMHLDSLGTDAIGTVQATDQGTIAGAVRLPLAGEGSH
                     TLLFYGDQTKTPAITDVSIVGFHPWVVLDNYSPGPEEQVGFAGSDFAPGETVYVFLND
                     LKSGPVDSVEVASDGSFKAPGALKLAAGMQGKQKLLFVGSQTQMASEADFQVLPYPGA
                     LQLTLYAGPPGSEVAFTGSGFSAGEEVTAYIGEPGNGTKVSTFKADAKGAFSGAGSFR
                     IPRDTKAGDVSLSVAGQVSGTQPSVSFKVLSLSPWAEVKQDRSGMAVVVGHGFAVGEA
                     VDLFVGGAGDKPVATAAADKDGNATFAPQEIRVESNKGAPVQLKGRDSGGEAKANYYG
                     GGAPESTPAAGGQPPAGQPTSGAGNQSGGIEFRPDTTPGAATPVPTAGR"
BASE COUNT         1254 a         2078 c         1949 g         1094 t
ORIGIN      
        1 ggcagcgggc acctccaccg tcatgctggg gaacctgttg gccggcacta ccgaggcccc
       61 ggggctcacg atccgtcgtg gcggccgccc ctacaagatc agccgcggaa tggcctccgc
      121 cgaggccgcg cttgccaggg ctgagcgcga acatcccgat cggggctggg cagagtggga
      181 agatgcgatc gaggatgtgg tgccggaggg agtggaggcc gtggtgccct accgcgggga
      241 cgttggagaa atcgtctttc aactggtggg gggcctcaga tccggcatga gctactgcaa
      301 cgcccgcacc ctggaggagc tgcggcagaa cgcgaccttc gtgagaataa ctgaggccgg
      361 tcgcaaggag agcgggcccc acgacatcga gataatgggg taagggggcc tctgatgtca
      421 cctgagagtt atgccggcag tgttcgagca cccgatttcc cagccggact ggattggctg
      481 aacgtcgatc acccgctcag tctggcagaa ttgcggggca agctggtgct gctggacttc
      541 tggacctacg gttgaatcaa ctgcatacac gtgcttccgc agttgcggaa gctggaagac
      601 gctttcccga atgagctggt agtagtggga gtccactccc ccaagttcca gggggagcgg
      661 ccgacgccca accttcgcca ggcagtcctc agaatgggga tccaacatcc ggtaatcaac
      721 gatcccggta tgcgactctg gtcggagtac agcgtgcgcg cctggcctac cgtgatgctg
      781 atcgatccgg agggcagggt gatcggcaat cacgaggggg agttccgatt cgaggagttc
      841 aggccgctga tcgaggccat gatctcggag ttcgaggcaa aggggaacct gaaccgacag
      901 ccgctgactc ttcggccgga agagcccccc gaaaccactc tcctgttccc cggcaagctg
      961 ctggcacatg agccatctgg acggctcttc gtagccgact cgggccacga caggatcctt
     1021 gtgtgcgggc tcgacggctc ggtacgccag gtaatcggcg gccagaaagg gttccgcgac
     1081 ggcgacattg ccggtgcagc cttcgacatg ccgctgggca tggcgctcga gggagatcgt
     1141 ctctacgtgg cggactgctg gaaccacgcc ctgcggcgca tcgacctgga ctcagggaga
     1201 gtggtgacag ttgccggcac gggcgagcag gcccgcaggg tcaaggaggg caacggccca
     1261 gaatccccac tcaactcgcc ctgggacctg gcaatccacc agggcactgt gtacatagcc
     1321 atggcagggt cccaccacat ctggagttac gacccacgat ccggacagat cggccgctac
     1381 gcggggagtg gccgcgaggc gctggttgat gggcccctag tgcgtgctac ctttgcccaa
     1441 ccctcggggc tgtccaccga cggccgcgca atgtacgtcg ccgacagcga gtccagcgcc
     1501 gtgaggaccg tagacctccc gggcgcagct ggagaggtgc gtacgctcgt agggcagggg
     1561 ctgttcgagt tcggggacgt ggacgggtcg ggtgacatgg tccgtctcca gcacaacctg
     1621 ggggtatgtt tcgacccgca ggaaggggtc gtcttcgtgg ccgacagcta caaccacaag
     1681 atcaagcagc ttgagccgcg cactcgccgc gttgatacga ttctgggcga cggagaggcc
     1741 gcactcaggg acggaattgg tgaagctgcg tccttctggg aacccggagg ggttagtgtg
     1801 gccggcggga ggttgtatgt ggccgacacg aacaaccacg ccgtccgggt taccgacctg
     1861 gcaacccgac aggtgaccac gctggagatc acgggagcgt aaagaggtcc cggcgcccga
     1921 ccgcattcca gaattcctgc gcctctgaca ccatgttacc cggtcgtact acagcccctc
     1981 agtatatact gcgttccgtc cgccttatcc tcgcatagta ctaaggtact atagccccgt
     2041 cattttatac cgtgttatac tgcgcccgtc cattagtaca tccctgcgca ctgatcggtt
     2101 gatttctagt ctctccctac cctccagcgg ccatagcgtc cctggagcac gacccctcga
     2161 tgaacccttg aagacgcaaa agcgggggtc caattcgacc gctggtctat tacaggagga
     2221 tttcgcttat gaaacgttgc cggttaccgc tcgtcctggc cctcggggca gttttggccc
     2281 tgatggtcgt gggctgcggc gccgtacaac gagggtctgc ccccggcgcc atcgcagcct
     2341 tgtggagtgc ctcttctacg gctggaggga atgccgacag cagcagcgcg actgacacca
     2401 aggccacggc cgccgacaag aacgcaagtg acagcagcaa gtcgaccgag aagaagtctg
     2461 cggcttccaa caagcccaat ttcctgagca ccaacggcac caggatcgtt gacgccgacg
     2521 gcaatgacgt gaagatcacc ggcgtcaact ggttcggcat ggagaccggc accttcgctc
     2581 ctcacggcat ctgggcgagg aattgggagg acatgctgga tcagatcgcc agcctcggtt
     2641 tcaacaccat caggcttccc tactccaacc aactcttcga ccctgagagc aaggtcggag
     2701 agggtgtcga catcaagctg aacccggaca tggaggggct cagcggcatg caggtcatgg
     2761 acaagatcgt cgagggcgcc ggcaagcgcg ggatcaaggt gatcctcgac cagcacaggc
     2821 cggacgtgaa cgcccagtcc aagctctggt acaccgacaa gtacagtgag gagcggtggc
     2881 tgtccgactg ggtcatgctg gccaagcgct acaagggcaa cgacaccgtc atcggtgccg
     2941 acctgcacaa cgagcccgcg ggcaacgcga cctggggcag cggcgacccg aagaccgact
     3001 ggcgaatggc agccgagaag gccggcaacg ccatcggcga agtgaacccc gattggctga
     3061 tcatagtcga ggggatcgag aagaccaaca acgacaagga ctggtactgg atgggtggta
     3121 acctgtccga tgcagcgaag tacccgatca aactgaaagt acccaacaag ttagtctacg
     3181 ccccccacga ctacggaccc gaggtttacg agcagggctg gttcaaggat acgagcttcc
     3241 cggccaacct gccggccgtg tgggatgccc actggggctt catcgccaag gacggcatcg
     3301 cccccctgtt gctcggggag ttcggcggaa agtccgtggg cgaagacgcc ggcggcacct
     3361 ggcagcgcag cctgatggac tacatcaagc agaacggctt gaactacacc tactggtcgt
     3421 tcaatgccaa ctccggcgac accggcggaa tactgacgga cgactggaag acggtcaatg
     3481 aggccaagtt gtccatgctt tccggctaca aggacaagat gatgcgagtg atgaatccat
     3541 ccaagatcga catcaccgcc gcacccagtg gcaagtccgg caagtagcca aggaaaagag
     3601 gcttcggtcg ggctgtcccg accgaagcct ctttttgtca ccgccgcacc accgcaaggg
     3661 tggccgcgtt gtccacctcg ccgtacagcc gcccgctatc ggcgcccacg acggtcaacc
     3721 gcagggcata ttggccctcc ggcacgctgt ccggaactcg ccactcccag tagatcgccc
     3781 tgctccccat ctccggaagc cggaacccgg attgcttcat cgtgagcacg ctgttcccac
     3841 cttcaacact cagcacgtca aggcgagcct gcagcggctc tgcaacggcg cccaaagcgc
     3901 tgaatcgact ggcgacggta acccgatcca acttctccac cgggtccggg gtcacggagg
     3961 cactaatgtt gacacagaca gggctcgcgg ccgccgcggc aagcgccgat gtcacgtgat
     4021 agagcgccag cccggagaac agggccacta gcgcaatcgg gactgctaac cttcgaagat
     4081 ccacaccgat cttcttcatc ttctcctcct gccaagttga gtgagattca cagtctctag
     4141 cgcgaaaccg gcacccacca actgctctcc ggcaactgcg gagcgtgccc cacacgcacc
     4201 agcaggtgat gagtcccatt cgaaggcaat ccggcgaaga aggccctgac taactggacc
     4261 ttctctccgg gcgccacgct caccatcccg acgtactcgg accacctcat gtcgtagctc
     4321 tgcccctgcc cgtcggtgac cgagagatcg gattctggaa gaaagctgaa ggtgactccc
     4381 ctctgggaac cgtttcgcag ttccaacagc agttccacgc caccctgtgc ggtgttaacg
     4441 tcgatgcttt ccagcgagac taccaacccg ttgccgatcg tgtgttcggc aggaagagag
     4501 aaatcggttg acgtatcgcg ggattcggaa acggggacgg taggcgttgt caccaacctc
     4561 ggctgcccac ccacccccgc gaccgtggtc acatcggcac tcgcatccga gacgggggtt
     4621 gccgcagccg aagcagtgct cccgcttgcg cccccgtctc catcgacacc tgtcgcatga
     4681 tctgtgccag gaagaaccgg ggcaagtgtc ggcaggggaa cgacggcagg ggcacccgat
     4741 ggtgcgggtg cccccagcag ggccacccca tatccgatca caaagccacc cacgatcagt
     4801 ccggtagtgg caaagagggc gaaccagatc gccctcaact ggcgctcacc cttccagccc
     4861 atcttcttcc accatcaacg ccccgccgta gggacgggag tcgccgcgcc cggcgtcgtg
     4921 tcaggccgga actctatccc tccgctctga ttgcccgctc cactggtggg ctggccggca
     4981 ggaggctgac ctccggcagc cggcgtactc tcgggtgctc cgccaccgta gtagttggcc
     5041 tttgcctcgc ctccgctgtc gcgacccttc aactgcaccg gtgcgccctt gttcgactcc
     5101 acgcgaatct cctggggagc gaaggtggcg tttccatcct tgtccgccgc ggcggtcgcc
     5161 acaggcttgt cgccggcccc tcccacgaat aggtccacgg cctccccaac ggcaaagccg
     5221 tggcccacca ctacagccat gcccgaacgg tcctgcttca cctcagccca ggggctgagc
     5281 gaaaggacct tgaagctcac gctgggctgt gtcccgctca cttggccggc gacgctcagg
     5341 gagacatcgc ccgccttggt atcgcgtggg attcggaagg aacccgcacc ggagaaggcc
     5401 ccctttgcgt ccgccttgaa ggtagacacc ttagttccat ttcccggttc gcctatgtac
     5461 gcagtcacct cctcgccggc cgagaaaccg ctccccgtga acgccacctc ggagcctgga
     5521 gggcccgcat agagggtcaa ctgaagcgcc ccagggtaag gtagcacctg gaaatcggcc
     5581 tccgatgcca tctgggtctg agaccccacg aagagcagct tctgcttccc ctgcatgccc
     5641 gccgccagct tgagggcgcc gggcgccttg aaactaccgt ccgacgcgac ctcaaccgag
     5701 tccaccggtc cggacttaag gtcgttcagg aagacgtaca ccgtctctcc cggcgcaaaa
     5761 tcgctcccgg cgaagcccac ctgctcctcc ggtcccggcg agtagttgtc cagcaccacc
     5821 caggggtgga agccgacgat ggagacatcg gtgatggcgg gcgtcttggt ctgatcgccg
     5881 tagaacagca gggtgtggct tccctcgccg gccagcggca gcctgaccgc cccggcgatg
     5941 gtcccctggt ccgtggcctg gactgtcccg atggcgtcgg ttccaagact gtccaggtgc
     6001 atggagacga tttcgccggg tgcgaaaccg ccaccgtcat agcccaccgc gccgccggtc
     6061 ttggcggtgt cggcggtcag cttggtccag gtctggtccg agataactcg gaggctggcc
     6121 tccaccgacg caccgccctt cgtcggggtg gcaaccacgg tgtagtcgcc cgtccctagt
     6181 tcctgaggca aacccaggga cgaatccgcc accaccccgt tcttgtcggc ggtcaccgtc
     6241 cccaatccaa ggggcttccc gtcgccggag ggctttatgg taatggatag ctcctggtag
     6301 ggctggaacg attgtccccc caccacaacc tcggcccccc gcttcacact tcctgcccgc
     6361 actattagaa acggg
//
LOCUS       DATALS010000142         7216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_193772, whole genome shotgun sequence.
ACCESSION   DATALS010000142 DATALS010000000
VERSION     DATALS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193772"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      29..170
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            273..1004
                     /gene="ribD"
                     /locus_tag="VHS06_03080"
     CDS             273..1004
                     /gene="ribD"
                     /locus_tag="VHS06_03080"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX2987135.1"
                     /translation="MADSYMAMALEQAKLARGNTGNNPAVGAVLVRDGRVVGRGFTQP
                     PGKPHAEIMALREAGELARGATLYVTLEPCCHYGRTPPCSDALIEAGIAEVHMATIDP
                     NPVVSGGGRAALERAGIPTHVGEMESEVRRFLEGWLSYISLGRPMVIAKYAMTLDGKI
                     ATFTGESKWISGPASRQRVQQLRASVDALMVGVNTVVADDPQLTGSAFRLAGSAALLR
                     YRRLGIGSVPHPALKDSINLATCLV"
     gene            1422..2618
                     /locus_tag="VHS06_03085"
     CDS             1422..2618
                     /locus_tag="VHS06_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987136.1"
                     /translation="MSQPTRTLNPLHFEDLEPHRFEDLVRQLAYGFRPWRQLEAVGRL
                     GRDDGMDIRGIEVADDRSISESSAALDAGYEAEEAAERVPIEEREWRIQCKRYKEIGP
                     KLMREIVQETVPDPQATPYGLIVAAACDVSAETMAAFRDELLSLAVAEGHLWTKAHLE
                     DMLFLPNNDHLLFAYFGISLGLRRQSRVQGIRRIIAIKGKLMRALKMASAEDYPDVQE
                     ILIRDVEDQDYPYDKNLPQFAQVIVPPWFVVRMDRFHPRGLLIEKNEYLGWVRTDGTW
                     DIITDSGHLTTMMGMLSRESQKSAKEQDAEEARWILWNKAPDQERTYIVEVRFIPYSV
                     IVEVDPIGDPWYSGPHVFCRFNGDDGPFWGEKLFLKGRGYDADSLLEESRRPLFAELQ
                     KEMNAD"
     gene            complement(2826..4256)
                     /locus_tag="VHS06_03090"
     CDS             complement(2826..4256)
                     /locus_tag="VHS06_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987137.1"
                     /translation="MELLERCTARSIAALSSWSPQLNLGVENLLRVPPTTDHEDSLEL
                     SRPHHLLRLSTEFHPEYLRRAQHVYANLLVPYWAVLKKGSVEGKFDLRGAESLVRSTG
                     AEFLLSGYDERIRNAIAHGEVVFRGAVTRYGPAVGGCEMIPYSFLEKFDELWRTSNAL
                     TVAILLFIARNRRLVMGNPDARLPPSITGLIADASIGRPSSLRVNGVVESEYRGTGRQ
                     LHVSVTTAFQSRIMTMADCARISLHLVEHSGDQYDRFLFEVDQGRQVPSLVIVDPRCL
                     ATLIAEQAPLDRLSEILIDTPLLFYDQSQLFLRARAWSLIFRSTFALAWDNLIAEWQR
                     IGIWRAKRRCVIRKITNASASGTVRLRIYAVLKYPSDADDQSMIKAIVKDIISSVWNS
                     WFDSRPDDLDRGWPRRGRPQYVWVHLYREDGTLRWLGKCGIRSGNLVAAAERVRGQRR
                     QPLFVKAADETWNGIRFRYGQDPQLT"
     gene            5425..5913
                     /locus_tag="VHS06_03095"
     CDS             5425..5913
                     /locus_tag="VHS06_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2987138.1"
                     /translation="MEPDLVLRKQLRALLDGQEAHMNVDSAVADFPMDRINDCAPNVP
                     YSFWHLLEHIRIAQWDILEFSRNPSHISPPWPEGYWPPRDARADEAAWKQTIASIRSD
                     MAALEELAANQSVKLYEAIPHGEGQTVLREVLLAADHTAYHVGEFAILRQVTGTWPPG
                     HE"
     gene            6077..7114
                     /locus_tag="VHS06_03100"
     CDS             6077..7114
                     /locus_tag="VHS06_03100"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX2987139.1"
                     /translation="MPISLMEDRGSVATSDFHYELVPNWEKLPAGYVHKDVVGIGVDS
                     KDRVIILTRNDAHVMVYDHDGTFVGAWPHHLFEKGRTHGVRVGPDDAVWIVDDKQHTV
                     RKFSPSGELLMMLGTPGVGSNSGYDSSKDGADLWTRLNTITRGGGPFNRPTGVAIAPS
                     GEFYVSDGYANCQVHRFTADGKLIQSWGEPGTGPGQFHLPHDVWVTKDERVFVCDREN
                     DRIQIFSKDGKFLDMWSHVQRPTSVIVGPDGLFYVSELSRRKGMLSYTNGVAPEDLPG
                     RVAILDEDGRVLHRWGTLDRTAPGQFCAPHCLCVDSRGDIYVGEVTWTDWGSKGLVPE
                     GTHMFQKFTRK"
BASE COUNT         1491 a         2111 c         2169 g         1445 t
ORIGIN      
        1 tcctatacta ggcatgtagc taagttaata gagtccttca gggcagggtg aggcacggag
       61 ccgattccca accggcggta tcgcagcagc gctgcggagc ccgcgagccg aaaggcagac
      121 ccggtgagat gccggggccg acggtacagt ccggagagaa gaaggcacgc aagcggttag
      181 atcggatcgg tccgaggctg ttgtgccttc gggccgtttt cttttgtctc ggtccacatc
      241 tgattacgaa tctctttgag agctgagagc caatggctga tagctacatg gcgatggcgc
      301 tagagcaggc aaagctggcc agggggaaca cgggcaacaa ccccgcggtg ggtgccgtgc
      361 tggttcgcga tgggcgagtg gtgggtagag gcttcaccca gccgcccgga aaaccgcacg
      421 ccgagatcat ggcccttcga gaagcgggcg agctcgccag gggggccacg ctgtacgtca
      481 cgctggaacc ctgctgccat tacgggcgaa ccccgccctg ctccgacgcc ctcatcgagg
      541 cagggatcgc cgaggttcac atggcgacca tcgatcctaa ccctgtcgtc agcggcggag
      601 gtagggccgc cctggagcgg gcgggcatac cgacccacgt cggagagatg gagtcggagg
      661 tccggcgctt cctggagggg tggctctcct acatttcgct gggccgtccc atggttatag
      721 ccaagtatgc catgacgctc gacgggaaga tcgccacctt tacaggggag tccaagtgga
      781 tcagcgggcc cgcgtcgagg cagcgagtgc agcagttacg cgcctcggtg gatgcgctga
      841 tggtgggcgt taacacggtc gtcgccgacg acccgcagct taccgggtct gcctttcggc
      901 tcgcgggctc cgcagcgctg ctgcgatacc gccggttggg aatcggctcc gtgcctcacc
      961 ctgccctgaa ggactctatt aacttagcta catgcctagt ataggaagac agcgagaggg
     1021 tgtcaaggaa agccggagat agaggagata aggggttccg gcaatgtcat gtcctggggt
     1081 ggagatggcc ggtgaggaag gtaatgactg gagcaagttt aggagacacc agacccggag
     1141 tcagatgact gcgtccctaa gagagtaagt acccagccgc gtccgttccc cccaccattt
     1201 catggcaaac tgcccacggt caccggctac tgccgttgtc gggagcgagg cagcgatcca
     1261 gcgaggaacg agcgtaacat ttgcctgtcg cggcggcctc acctcgcccg gcttaccacg
     1321 accaccttca catagaggag agaggcacgt gcgcttcggg tggagtgcct ggcgccgtgc
     1381 ctccggttag aatctctgta agtggaggtt ggaacaaaga gatgagccag cccacccgta
     1441 ccctaaaccc gttgcacttt gaggatctgg aaccgcaccg tttcgaggat ctcgtgcgtc
     1501 agctcgccta cggcttccgt ccatggcgac aactggaggc agtcggtcgc ctcggtcgag
     1561 atgatggcat ggacattcga gggatcgagg tagcagacga cagatccata tctgagtcta
     1621 gcgcggctct cgatgctgga tacgaggctg aagaagctgc ggaacgagtg cctatagagg
     1681 aacgggaatg gcggatccag tgcaagcgct acaaggagat cggtccaaag ctcatgcggg
     1741 agatcgtcca agagacggtg ccagatccgc aggcgacacc gtacgggctg attgtggccg
     1801 cagcctgcga cgtctcagcc gagaccatgg cagccttcag ggacgagctg ctatcactag
     1861 cagtagccga gggtcacctg tggaccaaag ctcacctgga ggacatgctc tttctcccca
     1921 ataacgacca tctgctattc gcttacttcg gcatctccct gggccttcgc aggcagtctc
     1981 gagttcaggg tattcggcgc atcatcgcga ttaaggggaa gctgatgcgg gcgctcaaga
     2041 tggcgtcagc ggaggactac cccgacgtcc aagagatcct aatccgcgat gtcgaggatc
     2101 aggactaccc atacgacaag aacttgcccc aatttgccca ggtgatagtg cccccgtggt
     2161 tcgtcgtccg aatggatcgg tttcatccca gaggtctgct tatcgagaag aacgagtact
     2221 tgggatgggt cagaaccgac ggcacgtggg acatcatcac agactccgga caccttacga
     2281 ccatgatggg catgctgagc cgagagagtc agaagagtgc gaaggagcag gacgcagaag
     2341 aggcccgatg gatcctatgg aacaaggctc cggaccagga gaggacctat attgtcgaag
     2401 tgcggttcat tccttattcc gtcattgtag aggttgaccc gatcggtgac ccttggtatt
     2461 cgggacctca cgtgttctgc cgctttaatg gtgatgacgg gccattctgg ggagagaagt
     2521 tgttcttgaa ggggagaggc tatgacgcgg acagtcttct tgaggagagc cggcggccac
     2581 tattcgctga gttgcagaag gagatgaacg cggattagta gtagtcgact tgaagctttg
     2641 ctatccgcca tcggtcaaag actctgcggt gatgcaattc cgcactacga tgcgattggc
     2701 tttcccccat cttccgctac ccatctacta gtcgaaggct ttccgaaatg gtttcgtggg
     2761 tctggcacgg cgacagcaca atggaatcct ccactccctt cctcagagtg gttctggaca
     2821 tccactcaag tcaactgcgg atcttgcccg taccggaacc gtatcccatt ccatgtttcg
     2881 tctgctgcct tgacgaacag tggttgccgc cgttgtccgc ggactcgctc agctgcggcg
     2941 accaaattcc ccgaccggat gccacatttg cccaaccacc gcagggtccc gtcttccctg
     3001 tacaagtgta cccagacgta ctgtgggcgc cctcgccgag gccaacctct gtccagatca
     3061 tcagggcgtg agtcgaacca cgagttccat acgcttgaga tgatatcctt cacaattgcc
     3121 ttgatcatgc tttggtcgtc ggcatctgac ggatatttga gaaccgcata gattcggagc
     3181 ctgacggttc cgctggcact cgcgttggtg atcttccgaa tcacgcagcg tctcttcgct
     3241 cgccagatac caatccgttg ccattccgcg atgagattgt cccaggcgag cgcgaaggtt
     3301 gacctaaaga tgaggctcca ggctctagca cgtagaaaga gttggctctg atcgtagaag
     3361 aggagagggg tgtctatgag aatctcgcta agtcggtcca acggtgcttg ctcagcaatg
     3421 agggttgcca ggcaccgcgg atccacaatg actaacgatg ggacttgcct cccctgatct
     3481 acttcgaaca agaaacgatc atactgatca ccgctgtgct caaccaagtg gagagagatg
     3541 cgcgcacagt cggccatagt cataatccgt gactggaagg cagtggtcac actgacatgt
     3601 agttgccttc ctgtgcctcg atactcagac tctacgacac catttacacg cagggaggat
     3661 ggtcggccga tactcgcatc agcgatgagc cctgttatgc tggggggaag tcgcgcgtca
     3721 ggattgccca taactagacg cctgttgcgt gcgatgaata gtaggatagc caccgtcagg
     3781 gcattggacg tgcgccacag ttcatcgaac ttctccaaga agctgtacgg gatcatctcg
     3841 cagccaccaa cagccggtcc gtatcgtgtc actgcgccac ggaagaccac ctcgccatga
     3901 gcgatagcat ttcttatgcg ctcatcataa ccggaaagaa ggaactctgc gcctgtgctc
     3961 cgtacaaggc tctcggcacc tcgcaggtcg aacttgcctt cgacagaacc cttcttgagc
     4021 acagcccagt atggaacaag cagatttgcg taaacatgtt gggcgcgtcg caggtattct
     4081 ggatggaact cggttgaaag gcgaagcaaa tggtggggcc tggagagttc aagggaatcc
     4141 tcatggtcgg tggtgggcgg caccctgaga agattctcga caccgagatt cagctgtggc
     4201 gaccaggatg acaacgcggc gatactgcgt gcagtacatc tctccagtag ttccaacgcg
     4261 ccttccaccg cagcaacgtc ccagtatcgc cacggtgcat gaattacaaa gcgagagagt
     4321 aggcgaggcc agagactgtt ggcatcagca ggtaagtagc gagagaagcg ttctagccct
     4381 cgctgtctca gcgcctcact ctcggctcgg gcaagaggat cgtccaggag gaaatccttg
     4441 atggttgaca atggagtgtt gctaccctgc acctctctta cgtctggtag cctcagctat
     4501 tccctctttg ctgagcaact tgcgagagcg gccctttaag gggatgcggc accttcaccc
     4561 ccgccggatc tccgaacaat aacgtcgact gattcctgtc gcgcgcatca tggcagaaag
     4621 aggtacatct ttcaagatgg gaaggatcag cagtttgatg acctctggat caggcatctc
     4681 accggagtac tctcgctccc gggcaagccg agcggctgct cgttagcgca ggttctcact
     4741 ccgaatcttg ctcgcttcac caccatgcgc tggatcatcg cccctcactc tcgctgccgc
     4801 caacgctgcc gcgcgtgccg cttcgaattc cggcaacgag gctgctcgct tgtgggtacg
     4861 gcgacatggt tcactgcaga agtcggctcc agtcctggtg agaggccggt cacactcgcg
     4921 acagtttcgt gacagtctca cctgtcggct gcggggtgcg gtggccattc catcgttgtg
     4981 tgggttgtgt ggtcttcttg cagttgggct ggttcgagtt attggagtcg gcaagagctt
     5041 ggagttggca ggcagcagtt tcccacttgg ttgtctgaaa gactgacata gttcctgggc
     5101 aaccctctcg gcaaccttgc cttcgtacgt cgtccaggtc ggtgtagtct gagccagcaa
     5161 gggtgaaagc ctggtcgtta ctcgcggtac tccttccatc gtctcaaaga agtcgccgat
     5221 tgtgaacgtg tgtttcttcg tgcctcttcc tgagacaatg gaaccctggt aggacttatc
     5281 accctgagtg ccggtgaaag atctcctcgt ggccggagag atccttcact ccgttcgaga
     5341 tgacacctgt cacgcccctc tctggccgga ttcgtgcaga ggtttgttgc agttgcccgc
     5401 gtcgcacgag taggaggtgt agaaatggaa ccggatctcg tcctgagaaa acagcttaga
     5461 gccctactgg acggccagga agctcacatg aacgtcgact ccgcggttgc cgacttcccg
     5521 atggatcgga tcaacgactg cgcccccaac gtcccctaca gcttctggca cctcctggag
     5581 cacatccgca tcgcccagtg ggacatcctg gagttctctc gtaacccgtc ccacatctcg
     5641 ccaccctggc ccgagggata ctggccaccc agggacgcca gggccgacga agcggcttgg
     5701 aaacagacca tcgcctccat aaggtcggac atggccgcgc tggaggagtt ggccgccaac
     5761 cagtcggtga agctgtacga ggcgataccc cacggcgaag ggcagaccgt cctccgagaa
     5821 gtgctcctcg ccgcggatca caccgcatat catgtcggtg agttcgccat cctgcgacag
     5881 gtgacgggga cgtggccacc aggccacgag tgacggtatc gagagcgcgc tcctgggctt
     5941 ttcggcgttg agcgaaacgt cgagaacagc gccgccagtt tggcgtacaa tagcggcggc
     6001 atcgaccgcc cggccgttaa ggcagcgcct ggccccactc tccctggcac gcaaccacat
     6061 ttccggggag cggccgttgc ccatttcgct aatggaggat cgaggaagcg tggccacgag
     6121 cgatttccat tatgaacttg ttcccaactg ggagaagctt ccggccggtt acgtccacaa
     6181 ggacgtggtg ggcattggcg tggactcgaa ggatcgcgtc ataattctta cccgaaacga
     6241 cgctcacgtg atggtatacg accacgacgg caccttcgtc ggcgcctggc cgcatcacct
     6301 gttcgagaag ggccgcaccc acggcgtccg cgtcggcccg gacgatgccg tctggatcgt
     6361 ggacgacaag cagcacaccg ttcgcaagtt ctcgccaagc ggcgaactgc tgatgatgct
     6421 gggcaccccc ggcgtcggct ccaactccgg ctacgactcc agcaaggatg gcgcggatct
     6481 ctggacgcga ctcaacacca tcactcgagg cggtggcccc ttcaaccgcc cgacaggcgt
     6541 ggccatagcc cccagcggcg agttttacgt ttcagacggc tacgccaact gccaggtcca
     6601 ccgcttcacc gccgacggca agctgatcca gtcctggggt gagcccggta cggggcccgg
     6661 ccagttccac ctcccccacg acgtgtgggt caccaaggac gagcgggtct tcgtctgcga
     6721 ccgcgagaac gatcgcatcc agatcttcag caaggacggc aagttcctgg acatgtggtc
     6781 ccacgtgcag cgtccgacct cggtcatcgt gggccccgat ggcctcttct acgtctctga
     6841 gctctccagg cgaaagggca tgctctccta caccaatggg gtggcccctg aggatctgcc
     6901 cggcagggtc gccattctcg acgaggatgg gagggttctg cacaggtggg ggaccctgga
     6961 ccgaacggct cccggccagt tctgcgcccc gcactgcctc tgcgtggact cccgtggaga
     7021 catctacgtg ggcgaggtca cctggaccga ctggggcagc aagggcctcg tgccggaggg
     7081 cacccacatg ttccagaagt tcacccgcaa gtagttgagt atgggcgacg gtctcccgtc
     7141 gcccatactg cattgcctgg tgcaccacca cagtagagat gatgtgtagg gcacaggcgt
     7201 gtacgcaacc cgagcg
//
LOCUS       DATALS010000143         3454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_371066, whole genome shotgun sequence.
ACCESSION   DATALS010000143 DATALS010000000
VERSION     DATALS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371066"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VHS06_03105"
     CDS             complement(<1..411)
                     /locus_tag="VHS06_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019265221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HEX2987140.1"
                     /translation="MDSEKTPRIGVYVCHCGGNISDVVDVKLVAERAGRLPGVVVSRT
                     NTFMCSDPGQEAIVEDIKSQHLDRVVVASCSPRLHELTFRNALRRAGLNPYLYEHANI
                     REQVSWVTHDHRAATDKASLLVAAAVGKARHLEPL"
     gene            complement(404..1810)
                     /locus_tag="VHS06_03110"
     CDS             complement(404..1810)
                     /locus_tag="VHS06_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HEX2987141.1"
                     /translation="MTNNDFSVDVLVVGGGISGLQASLDLADQGFQVAVVEKEPSIGG
                     KMIALSKVFPTLDCSSCITTPKMAASAHHSNIRILTYTELKGIEGEPGAFRARVLKKP
                     RYVDEENCIGCRQCEYACPISVPHSFEGGFGTRRAAYVPFTNAIPQLALLDMDNCVLC
                     GKCEKVCPANAIDFSQRPEEITISARSVVAATGFELTPPTAKPQYGYDSVPNVVTGLQ
                     MERLLAPHGPYGRVLRPSDGKMPESIAYVQCAGSRDASLGVPYCSRVCCMYAIKQAML
                     LSGALPLADITIYYMDIRAFGKGYEQFYQNAQAMGIEFVKGKVARITEGENQSPIVRV
                     EMIEDGGMVRERQHDLVVLSTGMLPGWHPEGIEGLTVAQDGFLRLPSAKLRPARTDRE
                     GVFVAGTAAGPKDIVDSIVEGGAAAMEASNFLRGLDAVGNGREAAGGVAATDPARPNG
                     TGHADVRETVAAGGRTNG"
     gene            complement(1807..2223)
                     /locus_tag="VHS06_03115"
     CDS             complement(1807..2223)
                     /locus_tag="VHS06_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HEX2987142.1"
                     /translation="METAVKAPVTNKILVLATLSCAYPGADTVGQAHMSYPANVFVMR
                     VPAPVLFPSQFYLDCFEKGVGGIIIMACGTDCPYEGAYKRLAERIDGLYRPMQERGLD
                     PRRIRLTSICTVCSGAFLKEVNQMNNLLAEIGAPQA"
     gene            complement(2235..2486)
                     /locus_tag="VHS06_03120"
     CDS             complement(2235..2486)
                     /locus_tag="VHS06_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX2987143.1"
                     /translation="MSQDLSTVQAAKVIDARGSACPGPLLEAKKAIGAVPVGGVLEIW
                     SNDPGTKKDIPLWAKKVGHEYVGFLVGEGYERIFVTRKK"
     gene            complement(2519..2890)
                     /locus_tag="VHS06_03125"
     CDS             complement(2519..2890)
                     /locus_tag="VHS06_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX2987144.1"
                     /translation="MGDSTEKIVIFGTHGGENPEKATLPFVVGNAALAMDVPVTIVLQ
                     AEGVTLVQKGCFEQIFAAGFDPLEKLVRSFVEFGGEILVCIPCVESRKIAHEQLLEAA
                     ELVKGARIVQEVLEAKAILNY"
     gene            complement(2995..>3454)
                     /locus_tag="VHS06_03130"
     CDS             complement(2995..>3454)
                     /locus_tag="VHS06_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX2987145.1"
                     /translation="SMIVFSGDLDKVLAAFIIANGAAAMDTPVTMFFTFWGLNAIRRP
                     EKVKVKKSLIESMFGWMMPRGPDKLTLSKMNMGGMGTMMMKSVMKQKNVYSLPKLIES
                     AKEQGVRIVACTMSMDVMGITAEELIDGVEFGGVASFLAAAGEGRTSLFI"
BASE COUNT          623 a         1133 c         1022 g          676 t
ORIGIN      
        1 cagcggctcc aggtggcgcg ccttgcccac ggccgccgcc accagcagcg atgccttgtc
       61 ggtggccgcg cggtggtcgt gggtaaccca gctcacctgc tcgcggatat tggcatgttc
      121 gtagagatag gggttcagcc ctgcccgccg cagggcgttg cggaaggtca attcgtgaag
      181 cctgggggag caggaggcca ccaccaccct gtccaaatgc tggctcttga tgtcctcgac
      241 gatggcttcc tgcccgggat cggagcacat gaaggtgttg gtgcgggaaa ccaccacgcc
      301 gggcaaccgg ccggctcgct cggccaccaa ctttacgtcg acgacgtcgg agatgttccc
      361 gccgcagtgg cacacgtaca ccccgatacg cggagtcttc tcactatcca ttggttcgac
      421 ctcccgcggc aacagtctcg cgaacatcgg catggccggt tccattaggc ctcgccggat
      481 cagtcgccgc cacgccgccc gctgcttctc gcccgttccc cacggcatcg aggccacgga
      541 ggaagttgga ggcctccatg gccgccgccc ctccctcgac gatggagtcg acgatatcct
      601 tgggaccggc cgcggtcccc gccacgaata ccccctccct atcggtacgg gcaggtcgaa
      661 gcttggcgct gggaagacgt agaaagccgt cctgggcgac ggtcagcccc tcgatgccct
      721 cgggatgcca cccgggaagc atgccggtgg agagcaccac caggtcgtgc tgccgctccc
      781 gcaccatgcc cccgtcttcg atcatctcca ccctcacgat ggggctctgg ttctcaccct
      841 ccgtgatgcg ggcaaccttg cccttgacga actcgatccc catggcctgg gcgttctggt
      901 agaactgctc gtaacccttg ccgaaggccc gaatgtccat gtagtagatg gtgatatcgg
      961 cgaggggaag tgcgccggat agcagcatcg cctgcttgat ggcgtacatg cagcagactc
     1021 gcgagcagta gggcacaccc agactggcat cgcgcgatcc ggcgcactgg acgtacgcga
     1081 tggactccgg catcttgccg tccgatggtc gcaagactcg accgtacggg ccgtgcggcg
     1141 ccagaagccg ttccatctgc aggccggtaa ccacgttggg cacactgtcg taaccgtact
     1201 ggggcttcgc cgtgggcggg gtcaactcga aaccggtcgc cgcaaccacc gaccgagccg
     1261 agatggttat ctcctcaggc cgctgggaga aatcgatggc attggcggga cacaccttct
     1321 cacacttgcc gcacaggacg cagttgtcca tgtccagcaa ggcaagctgc gggatggcat
     1381 tagtgaaggg gacgtaggcc gcccgacgag tgccgaagcc gccctcgaag gagtgtggga
     1441 cgctgatcgg gcatgcgtac tcgcactgcc tgcagccgat gcagttctcc tcgtccacgt
     1501 agcgcggctt cttcagcacc ctggctcgga aggctccagg ctcaccctcg atccccttca
     1561 gttcagtgta ggtcaggata cggatgttgc tgtgatgggc ggaggcggcc atcttgggcg
     1621 tggtgatgca actggagcag tccagcgtgg ggaacacctt gctcagggcg atcatcttgc
     1681 ccccgatgct cggctccttc tccaccacgg ccacctggaa cccctgatcg gcaaggtcca
     1741 gggaagcctg cagcccgctg atgccgcctc caaccaccag gacgtcgacg ctaaaatcgt
     1801 tgttcgtcat gcctggggag ctcctatctc tgccagcagg ttgttcatct gattgacctc
     1861 cttgaggaag gcaccggagc acacggtgca gatggaggtc agtctgatgc gcctggggtc
     1921 gagtcccctc tcctgcatcg gccggtacag accgtcaatc cgctccgcca gacgcttgta
     1981 ggcgccctcg tagggacagt cggtgccgca ggccatgatg atgatccctc ccactccctt
     2041 ctcgaagcag tccaggtaga actgggaggg gaacagcacc ggggccggca cccgcatgac
     2101 gaacacgttg gctgggtagc tcatatgggc ctggccgacc gtgtccgccc cggggtaggc
     2161 gcaggagagg gttgccagga ccagtatctt gttggttacc ggggctttga cggcggtttc
     2221 catgccctat gcctctactt tttccgggtc acgaagatgc gctcgtagcc ctcgcccacc
     2281 aggaagccga cgtactcgtg acccaccttc tttgcccaga gggggatgtc cttcttcgtg
     2341 ccggggtcgt tggaccagat ctccagcaca ccgccgaccg gcacggcgcc gatggccttc
     2401 ttcgcctcga gcaacggacc ggggcaggcg ctcccgcgag cgtcaatcac cttcgcagcc
     2461 tgtacagtag atagatcctg agacatcttg ctactcctta cgctgttctc gtttgatact
     2521 agtagttcag tatggcttta gcctctagaa cctcttggac aatccgggca cccttgacca
     2581 gttcggcagc ttcgagaagc tgctcatgag cgatcttgcg tgactcgacg cagggtatgc
     2641 agacgaggat ctcgccgcca aactcgacaa aggatcggac caacttctcg aggggatcga
     2701 accctgcggc gaagatctgc tcgaagcacc ccttctgcac aagggtcaca ccttccgcct
     2761 gaagcacgat cgtcaccggg acatccatgg ccagggcggc gttgcctacg acgaagggaa
     2821 gagtggcctt ttcaggattc tcgccgccgt gggtaccgaa gatcactatc ttctctgtgc
     2881 tgtcacccat tgaggttgcc ctccttatct cttctgcata cgtccgaggc gccgaacgcc
     2941 tcgtcggacg actcgcctag aggccttcca cgttccggcc tcggtccggc gggcctagat
     3001 gaacagggat gtcctgcctt cgccggcagc ggccaggaaa ctggccaccc caccgaactc
     3061 gaccccgtcg atcaactcct cggccgtgat ccccatcacg tccatggaca tggtgcaggc
     3121 aacgatgcgt acgccctgct cctttgcgct ctcgatcagc ttgggcaggc tgtacacgtt
     3181 cttctgcttc atcacggact tcatcatcat tgtgcccatg ccgcccatgt tcatcttgga
     3241 gagggtcaac ttgtcgggcc ccctgggcat catccagccg aacatggact cgatcaggct
     3301 cttcttcacc ttcaccttct ccggccttct gatggcattg agcccccaga aggtgaagaa
     3361 catggtgacc ggcgtatcca tggcggcggc tccgttggcg atgatgaagg ccgccaaaac
     3421 cttgtcgaga tcgcccgaga agacgatcat cgac
//
LOCUS       DATALS010000144         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_148086, whole genome shotgun sequence.
ACCESSION   DATALS010000144 DATALS010000000
VERSION     DATALS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148086"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VHS06_03135"
     CDS             <1..232
                     /locus_tag="VHS06_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987146.1"
                     /translation="LLEKDRTEYEAIAKTAKDGNTSVEYGSSEIPLREALNRTYSDLT
                     SVTQQIAALDKQIGDLPAATSAAGRLVARLQK"
     gene            519..1586
                     /locus_tag="VHS06_03140"
     CDS             519..1586
                     /locus_tag="VHS06_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX2987147.1"
                     /translation="MEIVELDDPQRETDDGREWDQLVAANPASGFMQSLAWAEFKRRQ
                     GYRTVRLGLEDDGSLVGGVQFFAAPQHHGLGLMIAPEGPILPWHDDKKSRTGLRLLVK
                     KAREMAPSIGAMGIRVEPRLPVPGVTAMRDFRRAPVDLVPRETLYLDLTQSPESLLRQ
                     MRPKGRYNIGLALRRGVAIREKSSADAVGAFYRVTAEAAERDQFFVEPFTFFVALAES
                     LCPAGMARFIFAEHEGDLLGAMLLVTFGDRATYLYGGTSNLKRNLMGGYALQWGAILA
                     AQRSGCRSYDFFGYEPFGAPSHLYSGFSRFKRQFGGEAQRFAGARDFFFLDRVADAVI
                     RASREIEAVAPMARSGEVGES"
     gene            1583..1960
                     /locus_tag="VHS06_03145"
     CDS             1583..1960
                     /locus_tag="VHS06_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987148.1"
                     /translation="MSEGANAAFGRIPSETQKLLQAVVQLVRDEIPSALPPNTGEAIK
                     ARTVEVVLRAVLKDWYQNYNVDGLREKDIDDLKSFVALAADLAGKDLNRTSQPIYEAL
                     LEALLEDWLINWNDGTDGPPERE"
     gene            1999..2220
                     /locus_tag="VHS06_03150"
     CDS             1999..2220
                     /locus_tag="VHS06_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX2987149.1"
                     /translation="MDLASEFHLAKIIAVVVWAAIGIAITYGGYRILDMLDPMDYQAE
                     IAKGNVAAAIKIAAFVLVVGAITIAAMVS"
     gene            2222..>2509
                     /locus_tag="VHS06_03155"
     CDS             2222..>2509
                     /locus_tag="VHS06_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987150.1"
                     /translation="MVPRTRNPQAHSLRTPEQTASPTAHGKYIVAPRIVTRRQESMLV
                     LGIGGAVFTLLSEWLTQGLTGVIVSERSMPNDGAIVVVVIALLVCWTLMAVP"
BASE COUNT          507 a          720 c          826 g          456 t
ORIGIN      
        1 cctcctggag aaggacagga ccgagtacga ggcgatagcc aagacggcga aggacgggaa
       61 cacgagtgtg gagtacggca gctcggagat cccattgagg gaggctctca atcgtacata
      121 ctccgacctg accagtgtga ctcagcagat cgccgcccta gacaagcaga tcggcgatct
      181 ccccgcggcc actagtgcgg cggggcgtct cgtcgcacgc ctgcagaagt gacctatgtg
      241 atccggaccc aaaggctgct tctgggatgg catcggcctc gcttggtccg gttcagttaa
      301 cgggacgagg acagcgtgga agaagcgtag ggcggggtct tgtgccccgc ctagagcgtt
      361 gccattgtca ccgtgccggt cgtgaccgcg gcgtgggaag gcggggcgca agaccccgcc
      421 ctacgagacg tcaagactgg cctgcttcgg gccaagcgtt cacagatcat gctgttgacc
      481 gaactgtgga agacggctcg acccgctgga caagaaacgt ggaaatcgtc gaactggatg
      541 acccacaaag agagacagac gatggcaggg agtgggacca actggtggct gcaaaccctg
      601 ccagcggctt catgcagagc ctcgcctggg cagagttcaa gcgcaggcag ggctatcgaa
      661 ccgtacggct gggtctcgag gatgatggga gtctagtcgg aggcgttcaa ttcttcgcgg
      721 cgccgcagca tcatgggctc ggactcatga tagcgccgga aggccccatt ctgccttggc
      781 acgacgacaa gaagagcagg accgggcttc gcctcctggt caagaaagct cgggagatgg
      841 ctccttccat cggcgctatg gggatacggg tggagccgcg cctaccggtg cccggcgtga
      901 cagcgatgcg cgatttccgc cgagcgcccg tggacctggt gccccgcgag acgctctatc
      961 tggatctcac ccagtcgccc gaatccctcc tgcggcagat gcggccgaag ggtcggtaca
     1021 acattgggct ggcccttcga agaggagtcg ccattaggga gaaatcgtca gcggatgccg
     1081 tcggcgcatt ctatcgggtt acggccgagg cggccgagcg ggatcagttc ttcgtggagc
     1141 cattcacctt cttcgtcgcg ctggccgaga gcctctgtcc tgcaggaatg gcacggttca
     1201 tattcgccga gcacgagggc gacctgctgg gtgcgatgtt gctggtcacc ttcggcgaca
     1261 gggcaaccta cctgtacggg ggaaccagca acctgaaacg caacctgatg gggggatacg
     1321 cactgcagtg gggcgccatc cttgcggccc aaaggtcggg gtgccgcagc tacgactttt
     1381 tcggatacga gcccttcggt gcgccgagcc acctctattc cggcttttcc aggttcaagc
     1441 ggcagtttgg gggtgaggct cagcgctttg ccggggcgcg cgacttcttc tttctggata
     1501 gggtggcaga cgcggtaatc agggcgagcc gagaaatcga agcggtcgcc cccatggcca
     1561 gatcgggcga ggttggtgag tcatgagcga aggtgccaac gcagctttcg ggaggatacc
     1621 ttccgagacc cagaagctac tgcaggcagt agtgcaactc gtcagagatg agattccctc
     1681 ggcccttcct cccaatacgg gagaggcgat taaggcgagg accgtcgagg tcgttctccg
     1741 tgcggtgctg aaggactggt accagaacta caacgtcgac ggtttgcgag agaaggacat
     1801 cgacgacctg aagagcttcg tcgccctggc cgcagatctc gccggcaaag atttgaaccg
     1861 cacaagtcaa cccatctacg aggcgttact ggaggcgctg ctggaagact ggctgatcaa
     1921 ctggaacgac ggcacggacg gtcctcccga acgggagtag gccgtccgaa gtcggcagaa
     1981 gactgaagga ggattgggat ggatctcgca tcggaattcc acttggccaa gatcattgcc
     2041 gtcgtcgtgt gggcggccat cggcatcgcc atcacctacg gaggataccg catcctggat
     2101 atgctggacc ccatggatta ccaggcagag atcgccaagg gcaacgtcgc cgctgcaatc
     2161 aagatagctg ccttcgttct ggttgttggt gccatcacca tcgcggcgat ggtgagctga
     2221 gatggtgcct cgtacccgga acccacaagc tcacagcctg cggacaccgg agcaaaccgc
     2281 atctccgacg gcgcatggga agtacatcgt tgcgcccagg attgtgacgc gccgccagga
     2341 gtcgatgctg gtgctgggca tcgggggcgc cgtattcacc ctactctcgg agtggctgac
     2401 tcaaggtctc acaggggtaa tcgtctctga gagatcgatg cccaacgacg gcgccattgt
     2461 cgtcgtggtg atcgcgctgc tggtatgctg gacgctgatg gccgtcccc
//
LOCUS       DATALS010000145         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_239169, whole genome shotgun sequence.
ACCESSION   DATALS010000145 DATALS010000000
VERSION     DATALS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239169"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VHS06_03160"
     CDS             <1..463
                     /locus_tag="VHS06_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2987151.1"
                     /translation="PEFMVLDEPTSALDVSVQAQILNLLRKLQREQRLTYLFISHNLS
                     VVRHMSDRIAVMYLGKIVEVTDRARLFTSALHPYTRALISAIPALAPRDRRERLRLVG
                     DVPSAIRVPSGCRFHTRCPFTQDRCRTEVPELRQVVLNGDSLHQVSCHRVS"
     gene            600..1706
                     /locus_tag="VHS06_03165"
     CDS             600..1706
                     /locus_tag="VHS06_03165"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX2987152.1"
                     /translation="MYRHADVSALGRGTVLYLKGSPYQQGKQLGRGAAELIHENLRLA
                     RNQRQEVASGLDQTDYNEVTRRNERWVSHEFPELLEEITGIAEGAEADYLELLSLNLN
                     SQLSYVYSTVLECTQALAMGPATIDGKTYVGKTRDLSRGPVLQVVLHREYEDGGFTNE
                     LKTAGRMTILDGINQHGVSLTCSGQWSPRVRVDVTRADSAWLLLNLQPVLRYARNTDQ
                     AVEMIREQPRASGMNVLVADGIKAVALEVTDRVAHQFEPENGLLVRTNHFFAPDLQPI
                     APTFDENRSTYDRHARASEILRERHGRVSMHDILGILSDHAAPPMESICRHGGADGQS
                     RTYAATINCPQDRTMWVTLGNPCEGIQAVGRPGK"
     gene            1959..>2728
                     /locus_tag="VHS06_03170"
     CDS             1959..>2728
                     /locus_tag="VHS06_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2987153.1"
                     /translation="MTANPASPSEDPKARPNAPVTRRSFIKLAAMAIGAPAALELLSA
                     CSPATPATSPTAPAAAGSGAPQPTAGSGAPQPTAAPAAKAASSFKPPIMVQPVDPETL
                     DPHFGESGIGMNVLDNLMETLVNYDRNMNLVPVLAESWQVMDDKVTWRFKLRQNVKFQ
                     NGEPFNAEAVKFTIDRTLNADLRSKGLNDPFPSRSGVTKVNVIDPYTVELVLKEPNII
                     LPVFLTFLYILEPKYYSSKSLQETALRPMGTGPWRVTD"
BASE COUNT          553 a          857 c          832 g          486 t
ORIGIN      
        1 accggagttc atggttctgg atgagcccac ctctgccctg gatgtctcgg tgcaggcgca
       61 gatcctcaac ctgctgcgga agctgcagag ggagcagcgg ctcacctacc tgttcatttc
      121 tcacaacctg tccgtggtca gacacatgag cgaccgcatc gcggtgatgt acctgggcaa
      181 gatcgtggag gtaaccgacc gagcgcggct cttcacctct gcgctgcacc cctacacgcg
      241 cgccctaatc tccgcgatcc ccgcgctggc tcctcgcgat cgtagggagc gtctgcgact
      301 ggttggggac gtgcccagcg ccataagggt gccatccggc tgccgattcc acacccgttg
      361 ccccttcacc caggatcggt gtcggacgga ggttcccgag ttgcgccagg tggtcctgaa
      421 tggcgattcg ctccatcagg tgagctgcca cagggtttct taagggaggc aatggagctc
      481 accgccacca ctgagactcg gaggtcgcag agccttcttt ggctatctcc gtgctacacg
      541 tatcagcctg gtgataatgg ccacccgcgg caagagcgag agaggagtgc aatcgagaga
      601 tgtatcgtca tgccgacgtc tcagccttgg gccgtggcac ggtgctgtat ctcaaaggta
      661 gcccctacca gcagggaaag cagcttggca ggggagcggc ggagctgatc cacgagaacc
      721 ttcgactcgc caggaaccag cgccaggagg ttgcttcggg cctggaccag accgactaca
      781 acgaggtcac ccgaaggaac gagcggtggg tgtcccacga gtttccagag ttgctggaag
      841 agattacggg catcgccgag ggcgcggagg cggactacct ggagttgttg agcctgaacc
      901 tcaactctca actctcctac gtctattcca cagtgctgga gtgcacccag gcgctggcga
      961 tgggtccggc gaccatcgat ggcaagacct acgtaggcaa gacccgcgac ctatcgcgcg
     1021 gacctgtgct tcaagttgtc ttgcaccgtg agtacgagga cggcggcttt acaaacgaac
     1081 tcaagaccgc ggggcggatg accatcttgg acggcatcaa ccagcacggc gtctccctga
     1141 cctgtagcgg ccagtggtcg ccccgagttc gcgtggatgt cacgcgagct gactccgcct
     1201 ggcttctgtt gaacctgcag ccggtcctca ggtatgcccg caacacggac caggcggtcg
     1261 agatgatcag ggagcagccg cgggccagcg ggatgaacgt tctggtggca gatggcatca
     1321 aggctgtggc tctggaggtg acagatcgcg ttgcgcacca gttcgagccc gagaatgggc
     1381 ttctcgtacg cacaaaccac ttctttgcac cggatctaca gcctatcgcc ccgaccttcg
     1441 atgagaatcg gagcacctac gacagacatg cgcgggccag cgagatactg agggagcgac
     1501 acggacgcgt cagcatgcac gacattttag gcatcctcag cgaccatgcc gcaccgccga
     1561 tggagagtat ctgccgacac ggtggtgccg atggtcaatc gagaacctat gccgcgacga
     1621 tcaattgccc tcaggatcgc accatgtggg tgacccttgg caacccttgt gaggggatcc
     1681 aggcagttgg gcggccgggg aagtaggggg aacagcatgt tatcggacga gctgttagta
     1741 cccagagtag tacctggttt cgatgtgtcg tggaggccgg gaacccatac tgaggaccgc
     1801 aaagccgagt atccataccg gatctctgtg atccgtgagc cccgagcgag tgaccccgcc
     1861 gacccattgc ctaggaagcg tctcggaagc cgaggcggcg gcttcaggcc gttatcctga
     1921 ggccgcgcat cctgcagcac gaattcggga ggtgacgcat gaccgctaat ccagcttctc
     1981 cttcggaaga tcccaaggcc cggcccaacg ccccagtgac tcgccgctcc ttcatcaagc
     2041 tggccgccat ggcgatcggc gcgccggccg cactcgagct gttgtcggcc tgttccccgg
     2101 cgacaccggc cacgagccca acggccccag ccgcggcggg ctcaggggcg ccccaaccca
     2161 cggccggctc gggagcgccc cagcccacgg cggcgccagc agccaaagcc gcctcgagct
     2221 tcaagccgcc cataatggtc cagccggtag atcccgagac gctcgacccc catttcggtg
     2281 agtccggcat cggcatgaac gtgttggaca acctaatgga gaccctcgtc aactacgacc
     2341 gcaacatgaa ccttgtaccg gtactggccg agtcctggca ggtgatggac gacaaggtga
     2401 cctggcggtt caagctacgc cagaacgtga agttccagaa cggcgagcct ttcaacgccg
     2461 aggctgtaaa gttcaccatc gaccgtaccc tgaacgccga tctacgctcg aagggattga
     2521 acgatccctt cccttcacgc tccggcgtca cgaaggtgaa cgtgatagac ccctacaccg
     2581 tggagctcgt gctgaaggag ccgaacataa tcctgcccgt attcttgacc ttcctgtaca
     2641 tactggagcc caagtactac agcagcaagt cgctgcagga gacggcgctt cgccccatgg
     2701 gtacggggcc atggcgtgtc accgactg
//
LOCUS       DATALS010000146         5815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_239220, whole genome shotgun sequence.
ACCESSION   DATALS010000146 DATALS010000000
VERSION     DATALS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239220"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VHS06_03175"
     CDS             complement(<1..604)
                     /locus_tag="VHS06_03175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2987154.1"
                     /translation="MTSTRDAPEKTLDSSGRQTHETTGHPSALRLGYLESAANNLAAA
                     ITIPFVVPYAVTLGADPTQVGLITALPLLAINLSQIPSAKWGAGSHRPGIFLLLGGAI
                     SRIGWLLLAITMLRGGADFPVLLAAAVAASLSGGLLSPIWTSFLGARVPVDRRGSYFG
                     TRNLLGGVASLAGASLAALLVSVAGFQRGYGFALLAATAFL"
     gene            complement(751..1344)
                     /locus_tag="VHS06_03180"
     CDS             complement(751..1344)
                     /locus_tag="VHS06_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2987155.1"
                     /translation="MVDPIDRYLREVVQMIEALPRDEISRVAQAVMQAREQQTQVFLF
                     GNGGSAATASHMACDLAKTSNVEGLNRIRAIALTDNAALMTAWANDVSYDEVFAEQLT
                     NLVQPGDLVIAISGSGNSPNVLRAVAVAKGAGAKTVGFTGQPGGQLKGMVDIAVVVPS
                     GRIEQAEDAHLILDHAISVALREAVTAPEAASGEEAA"
     gene            complement(1430..2512)
                     /locus_tag="VHS06_03185"
                     /pseudo
     CDS             complement(1430..2512)
                     /locus_tag="VHS06_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            2590..3747
                     /locus_tag="VHS06_03190"
     CDS             2590..3747
                     /locus_tag="VHS06_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX2987156.1"
                     /translation="MRIVDVKAYPLRVETAKPVGSALSMGRERSACLVEVITDEGLIG
                     WGEAGAGRSQEVLAAVVERQFRPMLLGEDPTDLNRLWDKMYSSLMVGGRTAGLSVQAL
                     SGIDIALWDLWGKSTGLPVYRLLGGKVRDSVLAYATAFYYHGGPDETEGVEEEALRYV
                     ERGFRAVKMKIGGEHPSVDVRRIRIVRKAIGPDRHLMLDANQAFTVAGALDLARRITE
                     YDISWLEEPLPAHDLQGYLQLRQGIGMPVAAGECLYTRFGFRDWCAQRAVDIVQPDLC
                     NAGGITECMRIASLTGAWDMLCNPHAWGTPVALAATIHLAVALPQVGHTRLQLPLVHS
                     PALECDQGENPLRDELAPLPFTFRDGFLTPTDGPGLGVDVDRVALKRFLVA"
     gene            complement(3847..4845)
                     /locus_tag="VHS06_03195"
     CDS             complement(3847..4845)
                     /locus_tag="VHS06_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987157.1"
                     /translation="MACLATLVMGSSAATLAYGAETRESVMPADGIFGSNLIVNGDAE
                     AGRGALFEDESHEVIPPPGWRTTGKLTAVVYVESDAFPTPDSPGPANRGANFLAGGPD
                     CDASSASQTIDVASGAAAIDGGKTTFVLDGYLGGYWDQEDSATLTATFRGADAKPLGS
                     ATLGPATVADRKGETGLFHYAKNGAVPVGTRSIEVVLNMVRANPYFNDGYADNLSLVL
                     TQPPAQPTAQPAAQPTAQPQQQSQPQPIAQTQAQPSFKLGFKALADQIPDVVGQPIED
                     EHWGANGDSLQQTTTGLMVWRKADNWTAFTNGSRSWINGPAGAQERGNDERFPWEQ"
     gene            5229..>5815
                     /locus_tag="VHS06_03200"
     CDS             5229..>5815
                     /locus_tag="VHS06_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2987158.1"
                     /translation="MASEATGKVILIMGSTDGIGRQTALDLARMGARVLLHGRNQQRG
                     QETLETIRRETGNDQLELYLADLSSRRQVRELANQIVGTHDRLDVVVNNAGVFEPARR
                     LTEDGVEMTFAVNYLAVFLLTQQILDLLKASAPSRIVNVSSGVHGTANLDWDNLQGEK
                     VYDGYQAYALSKLSDVIFTLELAERLRNTGVTANCL"
BASE COUNT         1039 a         1751 c         1919 g         1106 t
ORIGIN      
        1 ttaagaatgc tgtcgcggcc agaagggcga agccgtaccc gcgctgaaaa ccggccacgg
       61 agaccaggag cgcagccaac gacgcgcctg ccagtgacgc gacacctccc aggaggttcc
      121 gggtaccgaa gtagcttccc ctacggtcaa ccggcactct ggctccgagg aaggaggtcc
      181 agatggggga gagcagtccg ccggagaggc tggccgctac ggccgcggcg agcaggacgg
      241 gaaagtcagc gcctccccgc agcatcgtga tcgccaggag gagccatcct atccgcgaga
      301 ttgcccctcc taacagcagg aatatcccgg gtctatggga accggcgccc cactttgcgg
      361 agggaatctg gctcaggttg atggcgagca gtggcagcgc cgtgatcagc cccacctgtg
      421 ttggatcggc gccgagggtg accgcgtagg gtaccacgaa ggggatcgtg atggcggccg
      481 ccaggttgtt tgccgcggac tccaggtagc ccagtctaag ggcagaggga tgccctgtag
      541 tctcgtgggt ctgcctgcca gaactgtcca gggtcttctc aggcgcgtcg cgagtgctcg
      601 tcaatgtgtc gtggctcttg gcgcagcatt ggggaatcga acaggatgga caggatgttc
      661 cggatgtaca ggatatgacc caatttgttg tggatctatc ctgtatatcc ggctaatcct
      721 gtgcatcctg tgaattgagg gttcgttcgt tcaagccgcc tcttcgccgg aggctgcttc
      781 aggtgccgtg accgcttctc tcagcgcgac ggagatggcg tggtccagga tcaggtgggc
      841 gtcctcggcc tgctcgatcc ttccgcttgg caccacgacg gcgatgtcga ccatgccctt
      901 cagttggccg ccgggttggc cggtaaaccc cacggtcttg gctcctgccc ccttggcgac
      961 ggcgacggcg cggagcacgt tgggcgagtt gccgctcccg ctgatggcga tgaccagatc
     1021 gcccggttgg accaggttcg tcaactgctc cgcgaagacc tcgtcatagg agacgtcgtt
     1081 cgcccaggcg gtcatcagag cggcgttgtc cgtcagcgcg attgctctga tccggttcag
     1141 accctccaca ttggaggttt tggcgaggtc gcacgccatg tgagaggccg tagcggcact
     1201 tccgccgttg ccgaaaagga acacctgggt ttgttgctct ctggcctgca tcacagcctg
     1261 cgccactcgg gatatctcgt cccgggggag cgcttctatc atctgaacca cttccctcag
     1321 atagcgatca atgggatcca ccactggcac cctccggcaa ctgctgttag cgggactcca
     1381 ccatcgacgc gagggccgtc gaaggcagga aatggttcat tagcaacgat tatgcccgcg
     1441 cgaaaccggt agcgatagcg atccctccca gcagaccggc gtcgtcgccg agcgcggccg
     1501 ggagtatctc caaacctcgc ctgcacgact ccatggcgcg gctctgcacc gttcccctga
     1561 tcgcatccca caggatgggg cctgcgttca ggctcacacc cccgccaagg atcaccttct
     1621 gagggttgaa caggtgggcg aggttcacga tgccgacacc gagggcgact ccgacatcgt
     1681 ggatcaaccg gcggcaggct aggtccccat gggtggcatg ctctgtaacc ctcttggcgg
     1741 tgatctcctc cggcgtcagg ctagacagcg acgtggggac gccgctctcc accatctccc
     1801 gtgcccgtcg ggcgatcgag gtgccgctgg ccaaccactc gaggcagccg gtgttgccgc
     1861 acttgcaccg ctctccgttc atgtccaccg acatgtgccc cacctcccct gcgaacccgc
     1921 cgaagccgag gaggagcctg ttgtccacga ttacgcctcc gccgatgccg gtgctcaccg
     1981 tgatgtatac catctggcta cagcccttgc cggcgccgta ccggtgttcc ccaaccgcag
     2041 ccaggttcgc gtcgttggcg acatggacgt tagtgcgata gcgagactcg aacagttgcc
     2101 tcaggggcac catatcccat cacggcatgt tgggtgggtc gtagatcacc cctgtgtttg
     2161 ggtccagcgg cccgggtgcc gcgatcgcaa tgcctgagac ggggccggtt ccgtttactg
     2221 agaccactgc ctcatccacc gctgcgtaga gggtgcccag cactggatcg agtcctcgct
     2281 ctgccctggt gaagtcggat cgccgccact ggagatctcc cttgcgagtg cctacggcaa
     2341 ccctgaaccg ggtcccgccg aggtccaggg cgatggctgg ggccgtggag gtctgtttca
     2401 tgttggacat ccttaatgga ggtaagaata acaaaaataa gtgtaacatc tgcaatgact
     2461 cttcggggaa tctcgaaagg cgggccgggc gtatcgattg ggggatccac atcgagggag
     2521 tggtagaatt gtggctcggc cggggcagag aacggcgccc gcggcactgt ctgggaggag
     2581 cggaagtcga tgcgcatcgt tgacgtcaag gcgtatccac tcagggttga gactgccaag
     2641 ccggtcggtt ccgcgctcag catggggaga gagcgaagcg catgcctggt ggaggtgatc
     2701 accgacgagg ggttgatcgg ctggggcgag gccggggccg gacgcagcca ggaggtgctg
     2761 gctgcggtgg tggagcggca gtttcggccg atgctacttg gggaggaccc caccgatctg
     2821 aaccgcctct gggacaagat gtactcgagc ctgatggtgg gcgggaggac cgccggcctg
     2881 tccgtgcagg cgctgagtgg gatagacatc gccctgtggg atctgtgggg gaagtcgacg
     2941 ggactgcccg tgtaccgctt gcttggcgga aaggtacgtg actccgtgct agcctacgcc
     3001 accgccttct actatcacgg cggcccagac gagacggagg gggtggaaga ggaggctctg
     3061 cgctacgtgg aacggggctt ccgtgctgtc aagatgaaga ttggcggaga gcatccttcc
     3121 gtggacgtgc ggcgcatccg aatcgtgcgg aaggcgatag gaccggaccg ccatctgatg
     3181 ttggatgcca accaggcgtt cacggtggcg ggcgccctcg acctggcacg ccgcatcacg
     3241 gagtacgaca tcagttggct ggaggagccg cttcccgcgc acgacctgca gggctacctg
     3301 cagttgcgac agggcatcgg catgccggtc gcggcggggg agtgcctcta cactcgcttc
     3361 ggattccgcg actggtgcgc gcagagggcg gtggatatcg tccagcccga cctgtgcaat
     3421 gcgggcggaa tcaccgagtg catgcggatt gcctccctta ccggcgcctg ggacatgctc
     3481 tgcaacccgc acgcttgggg gacgccggtg gcgctggctg cgacgatcca cctggccgtg
     3541 gcgcttccac aggtggggca caccaggctg cagttgcccc tcgtccactc gcctgcgctg
     3601 gagtgcgatc agggcgagaa cccgctgcgg gacgagttgg ctcccctgcc tttcacgttc
     3661 cgggacgggt tcctgacccc cacagacggg ccggggctgg gtgtggatgt ggatcgcgtt
     3721 gccctcaagc gcttcctggt ggcgtagcag cgagaacgcc gaaggttgag ggcctggcat
     3781 gagtagtgac gcgccaggcc cccaagcacc agttaagact tcgcatcaga gcacgaacgg
     3841 cgctgactac tgttcccagg ggaacctctc gtcgttaccc cgctcctggg cgcccgcagg
     3901 cccgttgatc cagcttcggg agccgttggt aaaagccgtc cagttgtcgg cctttcgcca
     3961 caccatcagc cccgtggttg tctgctggag ggagtcgccg ttggcgcccc agtgttcgtc
     4021 ttcgatgggt tggccgacca catcagggat ctggtcggcg agggccttga accccagctt
     4081 gaacgatggt tgggcctgag tctgggcaat tggctgcggt tgagactgct gctgaggctg
     4141 ggcagtcggt tgtgccgcag gctgggccgt cggctgtgcc ggcggctgag tcagcaccag
     4201 gctcaggttg tccgcatagc cgtcgttgaa gtacgggttg gcgcgtacca tgttcaggac
     4261 gacctcgatg gagcgcgtcc cgacgggaac ggcaccgttc ttggcgtagt gaaacagccc
     4321 cgtctcccct ttgcggtctg cgaccgtggc aggtcccagc gtcgcgctgc cgagcggctt
     4381 cgcgtccgcg ccgcggaagg tggcggtcaa cgtcgcgctg tcttcctggt cccagtatcc
     4441 cccgaggtag ccgtcaagca caaaggttgt cttgcccccg tctatcgccg cagcaccgga
     4501 agccacgtcg atagtctggc ttgcgctcga tgcatcgcaa tccggccctc cggccaggaa
     4561 gttggccccg cgattagcag gacccgggga gtcgggggtg gggaatgcat cgctttcgac
     4621 gtatacgaca gcggtgagct tccctgtggt ccgccagccc ggcggcggga ttacctcgtg
     4681 tgattcgtct tcgaagaggg ctccacggcc ggcctcggcg tcgccgttga ctatcaggtt
     4741 actcccgaag atcccatcgg ccggcatgac gctctcacgc gtctcggccc cataagctag
     4801 ggtggcagcg ctgctaccca tgaccagagt tgcgagacag gccaaccgca gggcccgcct
     4861 ccatgcaaac gtcgtgtccc aggacatctt cgtcttctct acgctccttt catctccatc
     4921 cttggccccg ctggtccaga ctttcacctt tgaaagccgg gccggcccat tactgagaaa
     4981 taagcctatc gcggagagga acaggcatca tcgtggaaac tatgtatctt gtggcgagaa
     5041 gacgttttgg aaagagatca tggtgagggg aactatgtag gctcggtcca attgaaggcg
     5101 acggtcgggc tgatacttgc atccccaaaa gcctaagggg cacgccggtg cccttggagg
     5161 ataaggtttc tggggtgtcg ggcggacgtc aacgaagcgg gttgaacccg aacagaaggg
     5221 ggagagggat ggcaagcgag gctactggta aggttatcct gattatgggg tccaccgacg
     5281 gtattgggag gcaaaccgcg ctcgacctgg ctcgaatggg tgcgcgggtg cttctgcacg
     5341 gaagaaacca gcagcgaggg caggagacac tcgagacgat ccgtcgagag acgggaaacg
     5401 accaactgga gctgtatctt gccgacctct catcccggcg acaggtgcgg gagttggcga
     5461 accagatcgt cggcacgcac gaccgtctgg acgttgtggt caacaacgcc ggggtattcg
     5521 agcccgccag gcggctaact gaggatgggg tcgagatgac ctttgccgtc aactaccttg
     5581 ccgtcttcct cctgacgcag cagatactgg atctcttgaa ggcgagcgcc ccatccagaa
     5641 tcgtgaatgt cagttcgggt gtacatggga ccgcgaacct cgattgggat aatctccagg
     5701 gagagaaggt ctatgacggc taccaggcct atgctctctc caagctgtca gacgtaatct
     5761 tcacccttga gttggctgag cggctgagga acacgggagt gaccgccaac tgcct
//
LOCUS       DATALS010000147         6787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_148307, whole genome shotgun sequence.
ACCESSION   DATALS010000147 DATALS010000000
VERSION     DATALS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148307"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VHS06_03205"
     CDS             complement(<1..542)
                     /locus_tag="VHS06_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX2987159.1"
                     /translation="MAVSSIVPVVQPKPRRTNLWSEGGKSLVGIVRSGSDLKESIRQG
                     LDLIGGPGRLIRPGDAVFLKPNFNSADPFPASTDMPFLRAFVELLREAGASKVTMGDC
                     SGRIYWPSRDQFSRMGATALAEEMGIELLVFEELADWVKVQVGGAGHNLGLGWALVSS
                     CCGPGPPDVSSIGEGRAASG"
     gene            complement(706..1044)
                     /locus_tag="VHS06_03210"
     CDS             complement(706..1044)
                     /locus_tag="VHS06_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987160.1"
                     /translation="MGLLDRVRSFFSDVSSGEVYVMYIYIRCKRCGEPIRLRMDRRFD
                     LELEFGETLVSGFVANKDILGRKCSNLIRVHVEYDSSHRQKNRDIEGGQFITREEYEA
                     GVAEQARPNP"
     gene            complement(1058..2416)
                     /locus_tag="VHS06_03215"
     CDS             complement(1058..2416)
                     /locus_tag="VHS06_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2987161.1"
                     /translation="MPNLDQDYVLHHSTSQKLYQRALEIYPSGITHDTRYLSPFPIYV
                     THAKGSHKWDVDGHEYIDYLVGHGALILGHGYPDVVEAVQQQVAKGTHYGACHELELE
                     WGDLVKQLMGMERVKFLSSGTEATMMAMRLCRAFTGKDKVLKFRGHFHGWHDYATIGV
                     DPPYDLPTSLGVPEAVQGTVLAIEPNNPELLDRTLTENKDIACAIIEPNGGHWGTTPV
                     TPDFLRQRREITQKHGVLLIFDEVITGFRHSPGGAQALYGIKPDLVTMAKILAGGLPG
                     GGIAGRADVLDQLCFRATPQETRRQRVAHPGTYNANPLSAAAGVAALKVVATGQPHEQ
                     ANKMGQRLRDGMNAVLQEADVEGRVYGELSYFHTYIGPGAQQLAGDSTSPAAHAQLVG
                     SNQAARMSLMVKMLEKGMHFMANGGMISQAHTEADIDRTIEAFREVVQAIKAQMPVPR
                     AV"
     gene            complement(2829..4130)
                     /locus_tag="VHS06_03220"
     CDS             complement(2829..4130)
                     /locus_tag="VHS06_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2987162.1"
                     /translation="MGIARGIGIPKPEVFYGWWIVAAGATLGAMHGGLYYYSFGAFFV
                     PLNAEFGWSRAQVGAAFSLGQLAQGILFPIAGLLIHRLGPRRAITIGVALAGIGYMLL
                     TTSHSLFAFYLMFMLVATGCAIGTTPLASMTAITNWFVRRRSTASGWMMAGYGLGGGI
                     GTFFLGWLVSNYDWRMAAALVGAIFWLVGIPVAQMVRRRPEEMGYLPDGDVSSPPAIP
                     VSRPPGSSLSVARRAARPEAPDLTLRQAVRGRAFWLIAVSVALRNLVGAAVAVHQLPF
                     LIDRGYSMEVAAGIAGGTLLTGVPGRVFFGWLGDRWERRWVLATCHALMGIGVLLVTA
                     APPGNTLWLGVSLLLIYAPAYGGGWPIANAIITDYTGRRHYPTIFGLCSGLAVIGSVL
                     GPVTAGYLFDASHSYTLALQMFAALLILGLPLFWLLRRPAQ"
     gene            complement(4155..4478)
                     /locus_tag="VHS06_03225"
     CDS             complement(4155..4478)
                     /locus_tag="VHS06_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX2987163.1"
                     /translation="MAKEPTIAQKTFGDIAPKLAQLTDDVLFGDVWERPGLSKRDRSL
                     VTVAALVALYRTNELPGHISRALANGVTREELIEAITHLAFYTGWPNAVTALGIARRA
                     LADAD"
     gene            complement(4471..4983)
                     /locus_tag="VHS06_03230"
     CDS             complement(4471..4983)
                     /locus_tag="VHS06_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2987164.1"
                     /translation="MTRLDRLARARSELMALVLAVPDDVAEEPVCGEWNLRCVLAHLS
                     GWDTYFTAAARLLRAGAEVPFRGDSIDEWNRQMVTERESKSWDEVREEFVRMGEHLLE
                     EYGRLDEGQWGQRFWPGIGATPEWAIRRVAEHYEEHLAGIKERLGGQPGGGIKSGGTG
                     MNLSEGDYNG"
     gene            complement(5176..6171)
                     /locus_tag="VHS06_03235"
     CDS             complement(5176..6171)
                     /locus_tag="VHS06_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2987165.1"
                     /translation="MQKRKLGTGGLEVSAIGLGCMGMSQSYGPAGDRRDLTGLIRSAV
                     ERGVTFFDTAEVYGPFTNEELVGEALAPFRGRVVIATKFGHSIHAQRERSWTGLDSRP
                     EHIKQAVEGSLRRLQVEAIDLYYQHRVDPNVPIEEVAGAVKDLIQAGKAKHFGLSEAG
                     VQTVRRAHAVQPVTAVQSEYSVWWRRPEEELLPALEELGIGFVPFSPLGKGFLTGKID
                     ASTAFDSTDIRNTIPRFTQEARKANRTIVELLGNIAGKRDATPAQIALAWLLAQKPWI
                     VPIPGTRRLERLEENLGAVEVELTSGDLREIEETAAHITIQGARYPEELERLTGR"
     gene            complement(6220..>6787)
                     /locus_tag="VHS06_03240"
     CDS             complement(6220..>6787)
                     /locus_tag="VHS06_03240"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX2987166.1"
                     /translation="SRWEAPRQPASYTMAPSICLVAQGAQRVWLGEEEFVYDASHSLV
                     NSVDVPVVAQVIEASRERPFLGLVLELDQRAIAQMMVDSNLPLPRVQETSRAIAISEV
                     SLSLLNAFQRLIDLLDDPGDIPILAPLIQREILYRLLVGEQGPRLRQISAAGSQSHEI
                     ARAEGQTSGLAATRSRHAKRHSGVACCR"
BASE COUNT         1184 a         2253 c         2067 g         1283 t
ORIGIN      
        1 ctgccactgg ccgcccggcc ctccccaatc gaggaaacat ccggcgggcc aggcccgcag
       61 caggaggata cgagtgccca acctagacca agattatgtc ctgcaccacc cacctgcacc
      121 ttcacccagt cagccagctc ctcgaagacc agcagctcga tacccatctc ctccgccagg
      181 gcagtggcgc ccatgcggct gaactggtcc cggctgggcc agtagatgcg gccggagcag
      241 tcgcccatgg tgaccttgct ggcgcccgct tcccgcagaa gctcgacgaa ggcccgcagg
      301 aagggcatat cggtggaggc agggaaggga tcggcgctgt tgaagttagg cttgagaaac
      361 accgcatcac ccggccggat gagccggccc ggcccgccga tgaggtccag gccctgcctg
      421 atgctttcct tgagatcgga gcccgaccgg acgatgccga ccagggactt gccaccttcg
      481 ctccacaggt tggtgcgcct cggcttgggc tgcaccacag gcactatgga actgaccgcc
      541 attgctcttc cctccctgct tctcgcggcc tcaggaacct aaccccctgg cccccttccc
      601 ctcagggctt cgacctgcga gggaaacggg gacgtatttc acgttttgag gctggtcccc
      661 ccccccttta ggggaggctg gttccccttc cctttaggga aggggctagg ggttaggtcg
      721 cgcctgctcg gccaccccgg cttcgtactc ctcccgggtg atgaactgcc cgccttcgat
      781 gtcccggttc ttttgacggt ggctggagtc gtactccacg tgcacccgga tcaggttcga
      841 gcacttccga cccaggatat ccttgttggc tacaaatccc gagactagag tctccccgaa
      901 ctccagctcc agatcaaaac ggcggtccat gcgcagccgg atcggctctc cgcaccgttt
      961 gcagcggata tagatgtaca tcacatacac ctccccgctg ctcacgtcgg agaagaacga
     1021 gcgcacccgg tccaggaggc ccatggcctg tctctcccta cacggcccgg ggtaccggca
     1081 tctgggcttt gatggcctgc accacctccc ggaaggcctc gatggtgcgg tcgatatcgg
     1141 cttcggtgtg tgcctgagag atcatcccgc cgttagccat gaagtgcatg cccttctcca
     1201 gcatcttgac catcagggac atcctggctg cctggttgct gcccaccagt tgggcatggg
     1261 cggcgggaga ggtcgagtcg ccagcaagct gctgggcgcc agggccgatg taggtatgga
     1321 agtaggagag ctcgccataa acgcggccct ccacatcggc ctcttgcagt acggcgttca
     1381 taccgtctcg cagccgctgc cccatcttgt tggcctgctc gtggggctgg ccggtggcca
     1441 ccaccttaag agcggccacg ccggccgccg ccgacagggg attggcgttg taggtgccgg
     1501 gatgcgccac tcgctggcga cgggtctcct gtggggtggc ccggaagcat agctggtcca
     1561 gcacatccgc ccgcccggcg atgccgccgc ccggcaggcc acccgccagg atcttggcca
     1621 tcgtcaccag gtcgggcttg atgccgtaca gggcttgggc gccgcccggg gagtgccgga
     1681 agccggtgat cacctcgtcg aatatcagca ggacgccgtg cttctgggtg atttcacgcc
     1741 gctggcgcag gaaatccggg gtgaccgggg tggtgcccca gtggccgccg ttgggctcga
     1801 tgatggcgca ggcgatatcc ttgttctcgg tcagggtccg gtccagcagc tcggggttgt
     1861 tgggctcgat ggccagcacc gtgccctgca ccgcctccgg cacgcccagg gaggtaggca
     1921 gatcgtaggg cgggtcaacg ccgatggtgg cgtagtcgtg ccagccgtgg aagtggcccc
     1981 tgaacttcag caccttatcc ttgccggtga aggcccggca caggcgcatg gccatcatgg
     2041 tcgcctcggt gccggagctg aggaacttca cccgttccat gcccatcagc tgcttcacca
     2101 ggtcgcccca ctccagctct agctcgtggc aggcgccgta gtgggtgcct ttggctacct
     2161 gctgctgcac cgcctccacc acatccgggt agccgtggcc caggatgagg gccccgtggc
     2221 ccaccaggta gtcgatgtat tcgtggccgt ccacgtccca cttatgagag cccttggcgt
     2281 gggtgacgta gatggggaag gggctcaggt agcgggtgtc gtgggtgatg ccgctggggt
     2341 agatctccag cgctctctgg tacagcttct gtgaggtgga gtggtgcagg acataatctt
     2401 ggtctaggtt gggcactcgt atcctcctgc tgcgggcctg gcccgccgga tgtttcctcg
     2461 attggggagg gccgggcggc cagtggcagg gatgcgaggc gcgctgccgg gcggccgggc
     2521 tgctcccagg cagggctata gtctagcatc cggcccccaa agtcaaggca gtgcggcttg
     2581 atgactccac caaagtactg gggagaggca accagcatgt cattccgagc gaagtgaagg
     2641 gtcttgatcg aagcaatgaa tcgctaccct tacgggctag catttcgaga ttcttcggtc
     2701 actacgctcc ctcagaatga catgttctgc tacccctcta tcagttccct ctatccgttg
     2761 gtgtaacctc ggattagggg ttcaccaaat gtggatcagt gctgtgctcg ccacagaata
     2821 tcgcatcgtt actgcgccgg gcgccggagt agccaaaaaa gaggcaaccc caagatcagc
     2881 agggcggcga acatctgtag agctaaggta tagctgtggc tggcatcgaa cagatagcca
     2941 gccgtcaccg gccctagcac ggagccgatc accgccaggc cgctgcaaag gccgaagatg
     3001 gtgggatagt ggcggcggcc ggtgtaatcg gtgatgatgg cgttggcgat gggccagcca
     3061 cctccgtagg cgggagcata gatcagcaac agcgacaccc ccagccacag ggtgttgccg
     3121 gggggagccg cggtaaccag caacacccct atgcccatca aggcatggca tgtggccagc
     3181 acccagcgcc gctcccaccg gtcgcccagc cagccgaaga acacccggcc ggggaccccg
     3241 gtgagcaggg ttccccccgc gatcccggcc gccacctcca tgctgtagcc gcggtctatc
     3301 agaaacggaa gctggtgcac cgctactgcc gcgcccacca ggttacgcaa ggcgacactg
     3361 acggcgatca gccagaacgc ccggcctctc accgcctggc gcagggtcag gtccggtgct
     3421 tcggggcgcg ccgcccggcg ggccacggag aggctgcttc ccggcgggcg cgagacaggg
     3481 attgctggcg ggctggagac gtcgccatcc ggtagatagc ccatctcctc cggccggcgg
     3541 cgcaccatct gggccactgg tatgcccacc agccagaata tcgctccgac cagggcggca
     3601 gccatccgcc aatcgtagtt ggataccagc cagcccagaa agaaggtgcc gattcccccg
     3661 cccaggccgt agccggccat catccagccc gaggctgtgc tacggcgacg gacgaaccaa
     3721 ttggtgatag cggtcatgga ggccagggga gtggtgccga tggcgcagcc ggtcgctacc
     3781 agcatgaaca tgaggtagaa cgcgaacagg ctgtgactgg tggtgagtag catgtagcca
     3841 atccccgcca gggccacccc tatggtgatg gcccggcggg gtccgaggcg gtggatcagc
     3901 aaaccggcta tcgggaaaag gatgccctgg gccagctggc ccagggagaa ggccgcgcct
     3961 acctgggcgc ggctccagcc gaactcggcg ttgaggggca cgaagaaggc cccgaagctg
     4021 tagtagtaca gcccgccatg catcgccccc agggtggcgc cagcagctac gatccaccag
     4081 ccgtagaata cctctggttt ggggatgcca atcccccgtg ctattcccaa ccgcgagtac
     4141 ctcctccgac gggttcagtc agcgtccgcg agggcacgcc tggctatccc gagcgcggta
     4201 accgcgttcg gccagcccgt gtagaaggcc agatgggtga tcgcctcgat cagttcttcc
     4261 cgcgtgaccc cgttcgccag cgcccttgag atgtgacccg gcagctcgtt agtgcggtag
     4321 agggccacca gcgcggccac cgtgacgagg ctccggtccc gcttggaaag cccgggccgc
     4381 tcccacacgt cgccaaacag tacgtcgtcg gtgagctggg cgagcttggg cgcgatgtcg
     4441 ccgaaggttt tctgcgctat ggttggttct ttagccattg taatctcctt ctgacaaatt
     4501 cataccggtg ccgccagact tgataccgcc acctggctgg cctcccagcc tctccttgat
     4561 cccggccagg tgctcctcat agtgctcggc cacccgcctg atggcccatt ctggcgtggc
     4621 gcctattccc ggccagaagc gctgtcccca ctgcccctca tctaggcgcc catattcctc
     4681 cagaagatgt tcgcccatcc tcacgaactc ttcgcgcacc tcgtcccagc tcttggactc
     4741 tcgctctgtc accatctgcc gattccactc gtcgatggag tcgcctcgga agggcacctc
     4801 agcaccggcg cgcagcagcc tcgctgcagc tgtgaaatat gtgtcccagc cggagaggtg
     4861 agccagcacg caccggaggt tccattctcc gcataccggc tcctcggcga catcgtcggg
     4921 taccgccagg accaaagcca tcagctcgct acgagctcgg gccaaccgat ctagcctcgt
     4981 catgtcctca cctccccagt tcgcggcctg acctttacac tggctcttac tcgtaccgcc
     5041 gagcttaagt gttggctttc aggcctctgt cagcagatga tttctcacca tatagaacac
     5101 tcctcatcca ttattgagtc gcgtccctta ccgcatcctg ggccgggaat acaattacag
     5161 aatacctctt gccagtcagc gaccggtcag tcgctccaac tcttcggggt accgggcccc
     5221 ttgtatcgtg atgtgggcag cggtctcctc gatctcacga agatcgccgg aggtcagctc
     5281 gacctcaact gctccgaggt tctcttccag gcgctccagc ctccgagtgc ccgggatcgg
     5341 cacaatccag ggcttctgag ctaagagcca cgcgagggcg atctgagcgg gagtggcgtc
     5401 tcgttttcct gcaatgttgc cgagcaactc gactatggtc cggtttgcct tccgagcctc
     5461 ctgagtgaag cgggggatgg tgttgcggat gtcggtgctg tcgaacgccg tgctcgcgtc
     5521 gatcttcccc gtgagaaagc ccttgcccag agggctgaac gggacaaacc cgatgccgag
     5581 ctcctcaaga gctggcaaca actcctcctc cgggcgcctc caccacactg agtactcgct
     5641 ttgaactgcg gtgaccggct ggactgcgtg ggcccgacgg accgtctgca cacccgcttc
     5701 ggaaaggccg aaatgctttg ccttgcctgc ctggatgagg tcctttaccg cccccgctac
     5761 ctcttcgatc ggcacgttag ggtccacccg gtgctgatag tagagatcga tcgcctcgac
     5821 ctgcaggcgg cggagcgagc cttcaacagc ctgctttatg tgttccggcc gactgtccaa
     5881 cccggtccaa ctgcgctccc tctgggcgtg gatgctgtgc ccgaacttgg tggcgatcac
     5941 cacccgccca cggaagggag ccagggcttc tcccaccagc tcttcgttgg tgaacgggcc
     6001 gtacacttca gcagtgtcga agaatgtgac gccgcgttct acggcagacc gaatcaggcc
     6061 ggtcagatcc cgcctgtccc cagccggacc atagctctgg ctcatcccca tacaacctag
     6121 tccgattgcg gagacttcca gcccgccggt tcccaatttg cgcttttgca ttgttactcc
     6181 tccgggtggc tcgtaatatg ccttacctct ttctcagggc tatctgcagc acgctacccc
     6241 agaatgcctc ttggcgtggc gcgacctggt agccgcgagg cctgatgtct ggccctccgc
     6301 ccgagctatc tcgtggctct ggctccctgc tgcgcttatc tgccgcagcc gggggccctg
     6361 ctctcccacc agcaggcggt agagtatctc ccgctggatg agcggggcca ggatcgggat
     6421 atcacctggg tcatcgagca gatcgatcag ccgctggaag gcgttgagaa gtgacaatga
     6481 gacttcgctg atcgcaatgg cccggctggt ctcctgtacc cgaggtaacg gaaggttact
     6541 gtccaccatc atctgggcga tagcgcgctg atccagctcc aacaccagcc ctaggaaggg
     6601 ccgctcccgg ctcgcctcga taacctgcgc tactaccggc acatccacgg agttgaccag
     6661 ggaatggctg gcgtcataca cgaactcttc ttctcccagc cacacgcgtt gggctccctg
     6721 ggccaccagg cagatgctcg gcgccattgt gtagctagcc ggctgtctgg gagcctccca
     6781 ccggctc
//
LOCUS       DATALS010000148         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_194377, whole genome shotgun sequence.
ACCESSION   DATALS010000148 DATALS010000000
VERSION     DATALS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194377"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VHS06_03245"
     CDS             complement(<1..792)
                     /locus_tag="VHS06_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987167.1"
                     /translation="MAGSQGMRAAGWITLVGAVVGMAGVALFSLALLPAGVLSRPVRA
                     QLVTGGGAFLILGGLVFSLGLVLYLFLPLAGGGARAKREYGSHRVVAACTVFAAILGN
                     LGASAYFLLLPHLLGSTVGRTDMSGRVLSPEGIVVAALSLDLALLAVVYLRIVRPGAV
                     TFEEMGLDFHGVFGRFLMGILFGLLLFAASSLVEYLLGLAGVRQTQQESFQSITRASP
                     NEFGLVLLAGAAVAPFAEEEHAMERMMTVKEVAAYLKLNPITVYRY"
     gene            complement(792..1244)
                     /gene="nrdR"
                     /locus_tag="VHS06_03250"
     CDS             complement(792..1244)
                     /gene="nrdR"
                     /locus_tag="VHS06_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX2987168.1"
                     /translation="MKCPYCAHDQTKVVDKRDMTAGSTKRRRLCARCGRRFSTYEQAE
                     AASLVVVKKDGRREPFDRGKLRAGLLKACEKRPISSETIESLLEEVETGCRCRDGMEV
                     LSTVIGELVMDKLRSLDKIAYIRFASVYRAFADLQSFEEELHNLLKTR"
     gene            1528..1737
                     /locus_tag="VHS06_03255"
     CDS             1528..1737
                     /locus_tag="VHS06_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2987169.1"
                     /translation="MIEEEHAMERMMTVKEVAAYLKLNPITVYRYAAQGKIPGFKVGS
                     SWRFKKEILDEWVINEAKRYSGTTV"
     gene            complement(1895..2302)
                     /locus_tag="VHS06_03260"
     CDS             complement(1895..2302)
                     /locus_tag="VHS06_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015206867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987170.1"
                     /translation="MARGNSAGDGRHTEVVEMAQHPDEYRKDLNPDYEAGQNYGAAGE
                     QAEEGAATAFDYKDMQELLPDWNGSDLRQLIIVPEGSRLQQGATYFDLRDPGKGEFTA
                     RGDMVAEPDSRLVPKDRTQYDLWNRLVSTGARR"
     gene            2536..3702
                     /locus_tag="VHS06_03265"
     CDS             2536..3702
                     /locus_tag="VHS06_03265"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX2987171.1"
                     /translation="MAKPLSTKITSMSGSATVQVMNKARALKQQGIDIMDLGGGDPDF
                     DTPQHIQNAAIESMKSGFTHYVASSGVPQLRKAIAKKLATDNGVEVDPDKGVIISSGG
                     KLALYCSLSALLNEGDEVILPEPAWVTYRPIIKLAGATTVPLNLTAENGFSLTESALK
                     ALVTPKTKAIIVNSPCNPTGKVLSRKEMEIIAKVAVENDLYVVSDEIYEKIIYDGHKH
                     ISPASLPGMVERTLTLNGHSKAYAMTGWRLGYVAGPEVLIKEVGKLQQQTATCAPSFV
                     QMGGLAALEGTQEPVTMMTAEYKRRRDFFVDMLNAIPHVSCFKPEGAFYVFPKFDLGK
                     SSIEVADMLLEKARVAGTPGSAFGPAGEGHVRFSIASAPEVLETAANRIREMLS"
BASE COUNT          860 a         1268 c         1145 g          770 t
ORIGIN      
        1 atacctgtag accgtgatgg ggtttaattt gaggtatgcc gccacctctt tgaccgtcat
       61 catcctctcc atggcatgtt cctcctcggc aaagggagca accgcggctc cggccaacag
      121 caccaggccg aactcgtttg ggctcgccct ggtaatggac tgaaaactct cttgctgagt
      181 ctgcctgact ccagccaatc ccagcaggta ctccacgagg ctgctagcgg caaagagcag
      241 caagccgaag aggatgccca tcaggaaccg gccgaagaca ccgtggaagt ctagccccat
      301 ctcctcaaag gtaacagctc ccggccgcac gatgcgcaga tagaccacgg ccagcagcgc
      361 gaggtccagt gagagtgccg ccacaacgat accctctgga gagagcaccc ggccggacat
      421 atcggtgcgc ccgaccgtgg agccgagcaa gtgggggagc aacaggaagt aggcactggc
      481 acccaggttg ccgagaatgg cagcgaagac agtgcaggcg gccactaccc tgtgcgaccc
      541 gtactcgcgt tttgccctcg ccccgccgcc tgcaaggggc aggaacagat agagtacgag
      601 tccgagagaa aacaccagac caccgaggat caggaaggcg ccgcccccag tcaccaactg
      661 agcgcgaaca ggccgcgaga gcacgccggc aggcagaagg gcaaggctaa agagggcaac
      721 accagccatg ccgacgactg cgccgacgag agtgatccac ccggccgctc gcataccctg
      781 ggatcccgcc atcaacgagt cttgagcagg ttgtggagtt cctcctcgaa ggactggagg
      841 tcggcaaagg cccgatacac ggaggcgaag cggatgtagg cgatcttgtc cagcgacctg
      901 agcttgtcca tcacaagctc cccgatgacg gtgctgagca cctccatgcc atccctacaa
      961 cggcagccgg tctcgacctc ctcaagcagc gactcgatgg tttcggagga aatcgggcgc
     1021 ttctcacacg ccttgagcag gccggctcgc agcttgcctc tgtcaaaagg ttcccgccgc
     1081 ccgtccttct tcaccacgac caggctggca gcctcggcct gctcataggt gctgaaacgg
     1141 cgaccgcagc gagcgcagag acggcgccgc ttggtggatc cagccgtcat atctcgcttg
     1201 tctacaactt tggtctggtc gtgtgcacaa tagggacact tcaaggtatt gtaagccagt
     1261 ccacaaaatg ctaaactatc cgcatcttag ttaccccgct ttgccttgtc aaggcaaacc
     1321 tctcaaggct accgtcctgc tcagctttgt atgtgcgcat ccatcctggt gaatccgatt
     1381 caattcggct gtgttctttt gccaggagcg gagtgggata ccgaagcagt taccttcaca
     1441 atggttcttg tacttgcaag aggggggact ctaggatatg atgcgccggc aagtgttcgc
     1501 tagcggcaat atgcttcgag gtgccggatg atagaggagg aacatgccat ggagaggatg
     1561 atgacggtca aagaggtggc ggcatacctc aaattaaacc ccatcacggt ctacaggtat
     1621 gccgcgcaag gcaagatccc tggcttcaag gttggaagct cctggcgctt caagaaggaa
     1681 atcctggacg agtgggtgat caacgaggcc aagcgatact ccggcaccac cgtctaacct
     1741 tccagcatca ggtgcgataa cggtcgcacg tcaccacacc acaccttttc tgctgattga
     1801 ctccgacaag cgtcgccgca tgcgacgtca ataggtacct taactggccc tccgtcctgg
     1861 gtttttgccg ggttgttaga gggtttgtag ttgtttatct cctcgcccca gtcgacacca
     1921 aacgattcca caggtcgtat tgcgtccgat ccttgggcac caatctgcta tccggctctg
     1981 ccaccatatc acctcgagca gtgaactcgc cctttcccgg atcccgcaga tcgaagtagg
     2041 ttgccccttg ctgcagccgg ctcccctctg gaacgatgat gagctgcctc agatccgagc
     2101 cgttccaatc ggggaggagc tcctgcatat ccttgtagtc gaaagccgtc gccgcccctt
     2161 cctccgcctg ctcgccggcc gcaccgtaat tctgtccggc ctcgtagtct gggttcaggt
     2221 ctttcctata ctcgtccgga tgttgtgcca tttcgaccac ctcagtatgc cggccgtcgc
     2281 cggcagagtt gcccctcgcc atccctcggg ggctcgtccg gcggcaagat ctgtaccggc
     2341 acagatcgcg agattctcgc gcgttgcccg ccgttacgcc gtggtgtaac atacttcctg
     2401 gtgcatataa tggctatttg tgccccagtc cgaacactca cggttgccga aggctccaag
     2461 gtccctcacg cctgcggctt cgggatcgac cgctgattgt ccgaattcgt cgatgaagta
     2521 tggaggtctc gtcgcatggc aaagccgctc tccacgaaga tcaccagtat gtccgggtcg
     2581 gctactgttc aggtgatgaa taaggctcgc gccctgaaac agcaggggat cgatatcatg
     2641 gacctgggtg gcggtgatcc cgatttcgat acgccacaac acattcagaa tgccgccatc
     2701 gagtcgatga agtcaggctt cacccactac gtcgccagca gcggcgtccc gcagcttcgg
     2761 aaggccatcg ccaagaagct cgcgaccgat aacggcgtcg aggtcgaccc tgataaggga
     2821 gtcatcattt cctcgggtgg gaagctcgcc ctgtactgct cgctcagcgc cctgctcaac
     2881 gagggcgacg aggtcatcct gccggaaccg gcatgggtta cctaccgacc gatcataaag
     2941 ctggccggcg cgaccaccgt gccgctgaac ttgaccgcag agaacggctt cagcctgacc
     3001 gaaagcgccc tcaaggcact ggtaacgccc aagaccaagg cgatcatcgt gaactccccc
     3061 tgcaacccca ccggcaaggt gctcagcagg aaggaaatgg agatcatcgc caaggtagcc
     3121 gtggagaacg acctgtacgt agtctcggac gagatctacg agaagatcat ctacgacgga
     3181 cacaagcaca tcagccccgc cagcctcccc gggatggtag aacggaccct gaccctgaac
     3241 ggccactcca aagcctacgc tatgaccggc tggcggctcg gctatgtggc cggtcctgag
     3301 gtgttgatca aagaggtcgg gaagctgcag cagcagacgg ccacctgtgc gccgtccttc
     3361 gtccaaatgg gcggtctggc cgctctggag ggcacccagg agccggtgac gatgatgacc
     3421 gccgagtaca agcgacggcg cgacttcttc gtcgacatgc tcaacgccat cccgcacgtg
     3481 agctgcttca agccggaagg cgccttctac gtcttcccga agttcgacct gggcaagagc
     3541 tccatagagg tcgccgacat gctgctggag aaggcccggg ttgccggcac ccccggctcg
     3601 gccttcggcc ccgccggcga gggccatgtc cgcttctcca tcgcctcggc ccccgaggta
     3661 ctggagaccg ccgccaaccg catcagggag atgctgagct agctacctca gacaccaaga
     3721 ggagtggagg cttcagccgg aaaccaccgg ccggcgcctc cactccttct tttcatccaa
     3781 ggcttcgaag tgccgcgccc ccgagagaac gaacaggatg tacaggatac accggataag
     3841 aaccatctat tcaattgtct ctgtcccatt ctagtgttgc aagtagtaac ataggcttct
     3901 acacagtgca atcctaaggt tacggcgtga tttggatggg aactgccgtc ccgcagttag
     3961 ggcaggccac ctcctgggtc cggcaggcgg gttgatggtc tggagcagca tggttcgctc
     4021 cacagccttg gctctatccg cct
//
LOCUS       DATALS010000149         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_371647, whole genome shotgun sequence.
ACCESSION   DATALS010000149 DATALS010000000
VERSION     DATALS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371647"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..717
                     /locus_tag="VHS06_03270"
     CDS             94..717
                     /locus_tag="VHS06_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX2987172.1"
                     /translation="MAGNNSAKHDPYAGMPAYDEQSGNLNVVIETPKESRNKFKYDDS
                     RGIFRLGGVLPAGAVFPFDFGFVPSTLAEDGDPLDMLVLMDESAFAGCLVAVRLIGVI
                     EAEQTESGGTTERNDRLIGVASESKDNAEVRSLDQVNEHLVGEIEHFFVSYNQMRGKV
                     FKPVGRFGPERARKLVDEGMARFKKPPAASGNGKDALDRKNSAAKAR"
     gene            complement(1011..2678)
                     /locus_tag="VHS06_03275"
     CDS             complement(1011..2678)
                     /locus_tag="VHS06_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX2987173.1"
                     /translation="MEPGAASAGVTILVWLLIAILVVAVFSKYVRVPYTVALVVAGLI
                     IALTPLHLTIQLTPDLILFIFLPALLFESSFNLHFADLRDNLRPVAFLAVPGVILTAV
                     FIAAAMHYALALDWPTAFLFGAIMSATDPISVLAIFRRVGAPRRLAVILEGESLFNDG
                     TSLVIFAIVLQMVEAHQVADAVVTVEQFVVVVAGALALGAVIGYGASLLLSRVNDYLV
                     ETSMTLVVAYGSYLLAERIGVSGVIAVVVAALIVGNYGRNTAMSPTTRLAVSSTWEFI
                     SFLANSLIFILIGIELDLVKMGQYLGPTVLAIVVVLAVRAVVVVGSSWVLRYIHRPIS
                     FRWQIVLVWGGLRGALALAMALSLPLGISPSQPFPDRELLQVMTFGVIIFSLLGQGLT
                     MGPLLRRLGMTDTSERQEQYEVLAARRGMALAAISQIGKLEASGSLGSDVAEGLRHVY
                     SSQAEDLGESLRALRLRDEDIREQYVRAIRRRVIQSQKTYVQQRYLEGTLSEEGMRSI
                     TVDLDAELHELDLPGEPPEDGTEQGEPRETAQPERAEEARPASGTGS"
     gene            2985..4409
                     /locus_tag="VHS06_03280"
     CDS             2985..4409
                     /locus_tag="VHS06_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX2987174.1"
                     /translation="MENGNGRRILPFVLLVVVVTFLAYGLGAATMLAAGQFNGSKGQP
                     PASATAGSSQNSTGQPTAGANPPAVVPQNVDPQFRSFWQTYQAVQDEFYGRPVDSQKM
                     SYGAAKGMMQSLGDDFSTFLTPQENKVIQSEMQGNFEGVGMYFEIRNGLPTVVAPIPN
                     TPAERAGLRAKDVILAVDGRDITKMTTDQIATLIRGPAGTKVRLTMKRGDQPPFDVDL
                     TRASIDVPAVTLKWEDGNIANIQVNIFGDKTTQELDQALKEAQDKKASGIVLDLRNNG
                     GGWVVAAQEMLGRFLPSNSVAFYESHKSDHSDDRPQMVIPNGPKLPDIPLVVLVNGGT
                     ASASELVSGALQDYGRAKLIGEQTFGKGSEQHVHDWPDGSSARITFAHWLTPKKRDIN
                     PKPTATPNAQGTPAPLPTFTPQPSVTVPAAQATATAEAQPLVPSLTDRGLTPDIVVVR
                     TEKDYAQDTDPQLDRALQLLKTGK"
BASE COUNT          910 a         1454 c         1353 g          709 t
ORIGIN      
        1 gatcgcacag tccagcctca catctatcgg gcccggttcc tgctttggaa ccgggtatct
       61 ttatgccttc tgcaatcgat agagaggaga gacatggccg gtaacaattc cgcgaagcac
      121 gacccctacg cgggcatgcc cgcctacgac gaacaatcgg gcaacctcaa cgtagttatc
      181 gagacaccca aggagagccg gaacaagttc aagtacgacg acagccgggg catcttcagg
      241 ctgggcggcg tcctcccggc gggtgcggtc ttcccgttcg acttcggctt cgtcccctcc
      301 actctcgcgg aggacggcga cccgctcgat atgctggtgc tgatggacga gagcgccttc
      361 gccggatgcc tggtcgccgt gcgcctgatt ggagtaatcg aggccgagca gaccgagagc
      421 ggcggcacga ccgagcgcaa cgaccggctg atcggcgtcg ccagcgagtc gaaggataac
      481 gcggaagtcc gctcgctcga ccaggtcaac gagcacctgg tgggtgagat cgagcacttc
      541 ttcgtctcct acaaccagat gcgagggaaa gtgttcaagc cggtgggccg cttcgggccc
      601 gagcgcgcca ggaagcttgt ggacgaggga atggccaggt tcaagaagcc cccggcagcg
      661 tccggtaacg ggaaggacgc gctcgacagg aagaattcgg cagccaaagc ccgctgaggc
      721 gagacaactc ggaaaaagga tacgtgccta ctttgtcatt ctgagtagtc atgaccctgc
      781 gggccaccat cgctaatgaa aatgggatgg attggggaca ccccacaccc cgacaggaag
      841 cgccctgcgc ccctattttc agaggagccg tcaggcgaag aatccaagga gccatgctga
      901 gtagcaagcc ggcattctag atccttcgct ccgctgcgct ccgttcagga tgacataggc
      961 gggcaggaaa ccgatccctg acccccgatc gccgacccct gccctggcgg ttagctgccg
     1021 gtacccgagg cgggacgcgc ctcttccgcg cgctcgggct gcgccgtctc cctcggctct
     1081 ccctgctcgg tcccgtcctc cgggggctcg cccggcaggt ccagctcgtg caactcggca
     1141 tcgagatcga ccgtaatcga gcgcatcccc tcctccgaca gagtgccctc gaggtagcgc
     1201 tgctgcacgt aagtcttctg cgactgtata acgcgccgcc tgatcgcccg cacatactgc
     1261 tcgcggatgt cctcgtcgcg caggcgtagg gcgcgaaggc tctcgccaag gtcctcggcc
     1321 tgggaggagt agacgtgccg cagaccctca gccacatcgg accccagcga gccggaagcc
     1381 tcgagcttgc cgatctgcga gatggcagcc agcgccatgc ccctgcgcgc cgcgagcacc
     1441 tcgtattgct cctggcgctc cgacgtgtcg gtcatcccca gcctgcgcag caggggcccc
     1501 atggtgaggc cttgcccgag gagcgagaag atgatgacgc cgaaggtcat cacctgcagt
     1561 agctcgcggt cggggaaggg ttgggacggg gatatgccga gaggcagact gagggccata
     1621 gcgagggcca gcgcgccgcg cagcccgccc cacacaagca caatctgcca ccggaacgag
     1681 atcggcctgt gtatgtaccg cagcacccag ctggacccca cgacgactac cgccctcacc
     1741 gcaaggacga ccacgatcgc cagcactgtc ggtcccaggt actgccccat cttcaccagg
     1801 tccagctcaa tcccgatcag gatgaagatc agcgagttgg cgaggaagct gatgaactcc
     1861 cacgtcgagg agaccgccag ccgcgttgtg ggcgacatcg cggtgttgcg cccgtagttg
     1921 cccacgatca gcgcggcgac cacgacggcg atcactcccg aaacgccgat gcgctcggcc
     1981 agcaggtatg agccgtacgc caccacgagg gtcatcgagg tctcgaccag gtagtcgttc
     2041 acccgggaga ggaggagaga cgcgccgtag ccgataaccg ctcccagggc gagcgctccc
     2101 gccaccacca cgacgaactg ctccaccgtg accacggcat cggccacctg gtgcgcctcg
     2161 accatctgca gcacaatggc aaagataacc agcgaggtgc cgtcgttgaa gaggctctcc
     2221 ccctcgagta tcaccgccag gcggcggggc gctcccaccc gccggaagat cgccagcacc
     2281 gatatcgggt cggtggccga catgatcgcg ccgaacagga aggctgttgg ccaatcgagg
     2341 gccagggcgt aatgcatcgc ggccgcgata aacacggccg tcaatatcac tcccggcaca
     2401 gcgagaaacg ccaccggacg caggttatcg cgcaggtcgg cgaagtgaag gttgaaggag
     2461 gactcgaaca ggagggccgg gaggaatatg aaaaggatca ggtcgggtgt gagctggatg
     2521 gtcaggtgga gaggcgtgag ggcgataatg agccctgcga ccaccagcgc cacggtgtag
     2581 ggcacgcgaa cgtatttgga gaagaccgcc accaccagta tggcgatgag cagccacaca
     2641 aggattgtga cgcctgcact agccgctcct ggctccattt ccgctccctc acatgccgcg
     2701 tgtatacgct ggcgaattat atcatgcgag cgctgagtgc cgagcagaaa aggcggggac
     2761 ttcgcgcttt catgtcgtgc ccattcatac ttcatacctc atacttcata cctggaatcc
     2821 tcggttgccc tcacgggcgt acattgttta taattgcctg tcatgccgcc aggcgacgaa
     2881 gccgggcgtg acgtttagta tatcgatcag tctgcaagac cgcccgcggc aggtgtttac
     2941 aaagcgcgtc cttccctacg tctattcact ctcaggagaa tgtaatggaa aacggtaacg
     3001 gcaggcgaat attacctttt gtcctgctgg tggtggtcgt gacatttctg gcctatggcc
     3061 tgggtgctgc gaccatgctc gcggccggtc aattcaacgg ctctaagggg caaccccctg
     3121 cctcggctac cgccggttct tcgcagaaca gcaccggcca accgaccgcc ggggccaatc
     3181 cacccgcggt ggtcccacaa aacgtcgacc cgcaattccg gtcattctgg cagacctacc
     3241 aggctgtcca ggacgagttc tacgggcgtc cggtcgacag ccagaaaatg tcctacggcg
     3301 ccgccaaggg gatgatgcaa tctctcggcg acgacttctc taccttcctc actccccagg
     3361 agaacaaggt tatccagagc gaaatgcagg gcaacttcga gggcgtgggg atgtacttcg
     3421 agatacgcaa cggccttccc accgtggtgg cccccatccc caacaccccg gccgagcgag
     3481 ccggtctgcg cgccaaggat gtgatcctgg cggttgacgg ccgcgacatc acgaagatga
     3541 ccaccgacca gatagccact cttattcggg ggcccgcagg caccaaggtg cgcctcacta
     3601 tgaagcgcgg cgaccagccc cccttcgacg tggatctcac ccgggccagt atcgacgttc
     3661 ccgccgtgac cctcaagtgg gaggacggca acatcgccaa catccaggtc aatatcttcg
     3721 gcgacaagac cacccaggaa ctcgaccagg cgctgaagga ggcccaggac aagaaggcca
     3781 gcggtatcgt gctcgacctg cgcaataacg gcggaggttg ggtggtggcc gcccaggaga
     3841 tgctcggccg cttcctcccc tccaacagcg tggccttcta cgagtcgcac aagagtgacc
     3901 attcagacga ccgcccgcag atggtgatcc ccaacggccc gaaactgcca gacattccgc
     3961 tggtggtgct cgtgaacggc ggcaccgctt cggcctcgga gcttgtgtcg ggcgcgctgc
     4021 aggactacgg ccgcgccaag ctgatcggcg aacagacctt cggcaagggg tccgagcagc
     4081 acgtgcacga ctggcccgat ggctcgagcg cccggatcac cttcgcgcac tggctgacgc
     4141 ccaagaagcg ggacatcaac ccgaagccga cggccacgcc gaacgcccaa ggcactcccg
     4201 ctccgctgcc gaccttcaca cctcagcctt ccgtgacggt tcccgcggcg caggcgacgg
     4261 caacagccga agcccagccg ctcgtcccca gcctgacgga ccgcggcctc acgcccgaca
     4321 tagtggtggt gaggaccgag aaggactacg cgcaggacac ggatcctcag ctcgaccggg
     4381 ctctgcagct cctgaagacg ggcaagtaag cgccggggca gtagct
//
LOCUS       DATALS010000150         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_239484, whole genome shotgun sequence.
ACCESSION   DATALS010000150 DATALS010000000
VERSION     DATALS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239484"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1519
                     /locus_tag="VHS06_03285"
     CDS             35..1519
                     /locus_tag="VHS06_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX2987175.1"
                     /translation="MVQGTSSSVGKSLLVAGLCRLLHQEGYRVAPFKAQNMALNAAVT
                     PDGLEMGRAQAVQAEAAGIEPSVDMNPILLKPEGDSRCQVVVMGKPQSTLHAREYYRE
                     RGRLWETVCSSIERLRSRFDVVLIEGAGSPAEINLRAGDLVNMRVALETHAPVLLAGD
                     IDKGGVFASLVGTMELLAPEERELIRGFVINKFRGDVSLLEPGLRFLESRTGVPVLGV
                     VPYLRGLLISQEDSAGLEEVATGKGLLDVAVIRLPRISNFDDFDLLGAEPAVGLRFVG
                     RAEKLGRPDLVILPGSKGTVSDLGFLRDTGLDRAISALASTGTPILGVCGGYQMMGER
                     ILDPNGMESGQRETAGLGLLPATTTFATEKRTVRVRARVEATDGPFASARGATIDAYE
                     IHMGSTLHRGETVFSLLGGGVPVPDGCIAANGLLMGSYLHGLFENESLRRALVDWLVE
                     RRGLSPLQHVQSVASRDSEYDRLAETLRQSLDLPALRRIIGLEG"
     gene            1523..2104
                     /gene="cobU"
                     /locus_tag="VHS06_03290"
     CDS             1523..2104
                     /gene="cobU"
                     /locus_tag="VHS06_03290"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX2987176.1"
                     /translation="MGKMVLILGGARSGKSSLAERMAEQAGPKVTFLATAQALDEEMR
                     QRIAAHKASRPREWRTVEEPLELAAAVESAVKESDTVLVDCLTVWVSNHLCRMEAVES
                     TPEWHRELEMLGQRLRDQASRIATAAACGGATLLLVSNEVGLGLVPDNPLGRAYRDLL
                     GAINRQMAAEAEQVLLLVAGIPLDVKRLALEMP"
BASE COUNT          446 a          783 c          895 g          416 t
ORIGIN      
        1 cccggaggtg acattcttgc cggctaagct gctaatggtg cagggcacga gctcatcggt
       61 aggtaagagc ttgctggtgg cagggctctg ccgcctgctc caccaggagg gctaccgtgt
      121 ggcccccttc aaagcccaga acatggcgct caacgccgcc gtgacacccg atgggctgga
      181 gatgggtcgc gcccaggccg tccaggccga agccgccggg atcgagccct cggtggatat
      241 gaacccaatc ctcctcaagc cggagggcga ctcccgctgc caggtggtgg tgatgggcaa
      301 gccccaatcg accctacacg cacgggagta ctaccgtgag cgaggccgac tgtgggaaac
      361 ggtgtgctcc tcaatcgagc ggctgcgcag ccggttcgac gtcgttctaa tcgagggcgc
      421 gggcagtcct gcggagataa acctccgcgc cggtgatctg gtgaacatgc gcgtcgccct
      481 ggagacccac gcgcctgtat tgctggcggg agacatcgac aaaggggggg tcttcgcctc
      541 cctcgtgggg acgatggaac tgctggcacc ggaggagagg gagctgatcc gaggcttcgt
      601 gatcaacaag ttccggggcg acgtctctct gctggaaccg ggactccgct tcctggagag
      661 caggaccggg gtcccggtgc ttggggtcgt gccctacctg cgggggctgc tgatctccca
      721 ggaggattcg gcggggctgg aggaggtggc taccggaaag gggcttctgg acgttgccgt
      781 gatcaggcta ccccgcatct ccaacttcga cgacttcgac ctgctgggcg cggagccggc
      841 cgtcgggctg cgcttcgtcg gtcgcgccga gaagctcggc cgccccgacc tggtgatcct
      901 ccccggcagc aagggcaccg tgtccgacct cggcttcctc cgagacacag gtctggatag
      961 ggcaatatcg gcgcttgcca gcaccggcac cccaatcctg ggagtctgcg gcggctacca
     1021 gatgatggga gagagaatcc tggaccccaa cggcatggag tccggccagc gggagacggc
     1081 agggctggga ctgctgcccg ccaccaccac cttcgcgacc gaaaagcgca cggttcgcgt
     1141 tcgggcaagg gtcgaggcaa cggatggtcc cttcgcctcg gcacggggcg ccaccatcga
     1201 cgcctacgag atccacatgg gcagcactct ccacaggggg gaaacggtgt tcagcttgct
     1261 ggggggtggc gttccggtac cggacggctg catcgccgcg aatgggctgc tcatgggtag
     1321 ctacctccac ggactgttcg agaacgaatc gctccggagg gcactggtgg actggttggt
     1381 tgagaggcgg gggctgtcac cgttgcagca cgttcagagt gtggcaagca gagactccga
     1441 atacgatcgt ctcgccgaga cgttgcggca aagcctggac ctgccggcgc tgcgacgaat
     1501 aatcgggctg gaggggtagt cgatgggcaa gatggtgctg atcctgggtg gcgcccgaag
     1561 cggcaagagc agtctcgccg aacggatggc cgagcaggct gggccgaagg tgaccttcct
     1621 ggccacggcg caggcgctgg acgaggagat gcgacagcgc atagcggccc acaaggcatc
     1681 tcgcccgagg gaatggcgaa ccgtcgaaga gccgctggag cttgccgcgg ccgtggagtc
     1741 tgccgtgaag gagagcgaca ccgtgctggt ggactgtctg acggtctggg tatctaacca
     1801 cctctgccgg atggaggccg tcgagtccac cccagagtgg caccgggagc tagagatgct
     1861 gggacagcgg ctgcgagacc aggcgtcgcg catagctaca gcagcggcgt gcggcggagc
     1921 cacgctgctg ctggtctcga acgaggtggg gctggggctg gtccccgaca acccgctggg
     1981 acgcgcctac agggatctcc tgggggcgat caaccgccag atggcggcag aggccgagca
     2041 ggtgctgcta ctggtggccg gcatcccgct ggacgtgaag cgactcgcgt tggagatgcc
     2101 ctgatggcgg atacacctca gtccgtaggg gcgacgcatg cgtcgccctc cccggcgagc
     2161 cgaccaaacc ttcgggcgac gcatgcgtcg cccctacgaa tcgaggatga ccgcgtggca
     2221 accgccaagg ttccctgcac ctgcggcgaa ctcgttcagg gagcgttggc cggcaggcca
     2281 ttcctcgtct cctgccccat cgaccgatac agcgaggtaa gggtgaggct cggacgaccg
     2341 aacgccgagg gcgcggcaac ggggagatgg acactcgatg ggcccccgga gccgtcctcc
     2401 ccttgccgtt tcctgtgaac accatcacga gccccttggt gcgggccttg ggcttatcct
     2461 ctgtcgtcat ctgttcctcc cgctacgagt tacgagtgcc gggtgctgag aaggattgta
     2521 tcacccgacc tgtgccggag
//
LOCUS       DATALS010000151         4742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_149056, whole genome shotgun sequence.
ACCESSION   DATALS010000151 DATALS010000000
VERSION     DATALS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149056"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VHS06_03295"
     CDS             <1..355
                     /locus_tag="VHS06_03295"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX2987177.1"
                     /translation="VFQEDATEDKLARLGGISSHLATQLARRIPQDVRFTVLGHLQRG
                     GSPTPFDRILGTRFGGAAVRLVAEGGLDKMVALHTPDIVPVELSEAIGSLKRVKPDGE
                     LVRTARDMGIFMGDE"
     gene            599..3733
                     /locus_tag="VHS06_03300"
     CDS             599..3733
                     /locus_tag="VHS06_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2987178.1"
                     /translation="MLLEGRDSWLQRLSLRTIQGFLLLVVLIPVLLLQAGIYLRQSDT
                     RRQEESQSSRLIAGIVASSTGAFLQRQLRYENSLGTAIAAGESMSTNRIRHLLNEQAG
                     VYSTYKTIGWIDDKGTVVASSRPVPAGSNANGRDYFLALQKGDDWAVSDLVTSALDGL
                     DVVLIARSVRDDNGRLRGAVVVAVEPGMLGEAIGFPELRQWNVAVLDTQGHVLFAYPR
                     LSADPAQAAWLSGATAKENELVATALRGQEATGTIPLTPRGESMIVTATPILPLGWVA
                     ISARPENEVMAPVREGLISDFALLILIAAGAFGLALYTSRAITDPITRLRDQADALAE
                     GDLLGQVEGGGPVEIDRLAIAFDRMAEQIQSREESLKEANWLLMAATVRNQQLAERAQ
                     ASLAQFEAIVESMTDGLILSDARGNILRMNPAALVIHGMDQLDSASLQFNSFEGLYQM
                     MEAPSGRPLTPTERPFARALAGESFSQLQVRVRPIGNETERAISYNGAPIRDRDGKIT
                     MAVVTMRDVTARRRAEAEREKLLAELDATISTLMSVADGVLLYDPAGKITQVNSVAQR
                     LLACGPNGEGLPAGLRATPPLIVQSSSATAQPNTIPARALKGIGTHAEVMRLQKNGST
                     TWMAVSAAPVLAKDGQKLGAVVTLTDITVLRELQERQEKLLRDVKASNEMLVQSSIRE
                     RTLAAEAQRQAAQMNALLEKLGEAVVVLDGAGNILVRNAKAEEIDPPPGANIRNGAEL
                     SPRSILQADGSIMPIEQLPQSRVLRGESFADYEVIYTEPGDTMRHLLFSAGSLRDETA
                     KVVLGILVYRDVTELRHLEQVRDDYMRAVSHDLRNPLTAIQGHAQLLRRLLEQDGVDT
                     KRLHFLDTIIANATRMNSMIKDLAESARLEAGQRTLECVPLDLPAFTLNLKERLSTPS
                     DAERIRVEAPPDLPLVWADEEALERILRNLLGNALKYSPADCPVIVTMASGDKEIVTS
                     VRDGGEGIATEEQQHLFQRYYRASSGKANRGGLGLGLFITKGLVEVHGGRIWVESEIG
                     KGSIFHFTLPVATR"
     gene            3841..4449
                     /locus_tag="VHS06_03305"
     CDS             3841..4449
                     /locus_tag="VHS06_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2987179.1"
                     /translation="MSHNIQWLGHDVFKISSNGIVIYTDPFRLRAGGDKADIILVTHD
                     HFDHYSPDDIAKIVRQDTAIVAPHSVADKLAGEARSSTSKLDAQKIEEVRPGNRLEVN
                     GIPIEVTWAYNVNKFRNPGEPYHPKSPDFVGYIFTVDGKRIYLAGDTDMIPEMNTIDV
                     DIALLPVSGTYVMTADEAIQAANSAVKSKSYIPMHYNASRGR"
     gene            complement(4418..>4742)
                     /locus_tag="VHS06_03310"
     CDS             complement(4418..>4742)
                     /locus_tag="VHS06_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX2987180.1"
                     /translation="LLTDHVQREIEITQIGESIRSRAEEQIGKSQREYLLREQLRAIQ
                     RELGEETEQAREVSDIRKRIEEAELPSEARQEAERELNRLEQLPAISPEHGIILTYLD
                     WRCSA"
BASE COUNT         1023 a         1440 c         1414 g          865 t
ORIGIN      
        1 ggtcttccag gaggacgcta cggaggataa gcttgcacgg cttggcggca tatccagcca
       61 tctggcaacc caactggctc gcaggattcc ccaggacgtg aggttcaccg tgctcggcca
      121 tcttcagagg ggaggaagcc caacgccctt cgaccgcatc ctgggaacgc gattcggagg
      181 agccgccgtg aggctagtag cagagggtgg cctcgacaaa atggtagcgc tccacacgcc
      241 ggacatagta cccgtggagc tgtcggaggc aatcggatct ctcaagagag tgaagccgga
      301 cggcgagctg gtcaggacgg caagggacat gggcatcttt atgggagacg agtagtctcc
      361 cgcgagttgt ccgatgacac caacctcttc attgtctata atgatcaggt agccaggagc
      421 aagagacggc aagggactgg gccttccgcg catccccacg cgcgcctctg ccgtctgcca
      481 cctccgcatc gagccatctt ctccgcgaac tccattggag gcctggatgt cggagtcgac
      541 cggttcgggc aatctaggtc attcgagcgg ggacaattgc cctccgtctt gtgatgctat
      601 gcttctcgaa gggagggata gctggctgca gcgactctcc cttcggacca tccagggttt
      661 tcttctcctc gtcgtactca tccccgtgct gctcctgcag gccggaatct accttcggca
      721 gtcggatacg cgtcgccagg aggagtctca atcaagcaga ctgattgcgg ggatagtcgc
      781 gtcctcgacg ggggcgttcc tgcagagaca actccgttac gagaactcgc tcggcacggc
      841 catcgccgcg ggcgaatcga tgagcaccaa caggatcaga cacctcctca acgagcaggc
      901 cggcgtctac tctacttaca agactatagg ctggatcgac gataagggca cggtagtcgc
      961 ctccagtcgc ccagttcctg ctggaagcaa tgccaacggc cgtgactact tcctggcgct
     1021 gcagaagggc gatgactggg ctgtcagcga tctcgtcacc tcggcgctcg acggtctgga
     1081 tgtagtactg atagccaggt cggtgcgtga cgacaatgga cggctgagag gtgccgtggt
     1141 cgtggccgtg gaaccgggaa tgctgggtga ggccattggc ttcccggaac tgagacagtg
     1201 gaacgtcgcg gtcctggaca cacagggaca cgttcttttc gcataccccc gcctatctgc
     1261 cgatccggcc caagctgcat ggctcagcgg cgccactgcc aaggaaaacg aactggttgc
     1321 cactgcactt cgtggccagg aggccaccgg caccataccc ctgacccctc ggggagagtc
     1381 gatgatagtc accgccacgc cgatcctgcc gttaggctgg gttgccatct cggccagacc
     1441 agagaacgag gtgatggcgc cggttcgcga agggctgata tccgacttcg cactcctgat
     1501 cctgattgcg gcaggagctt ttgggctggc actttacacg agccgcgcca tcaccgaccc
     1561 catcactcga cttcgagacc aggccgacgc ccttgccgaa ggtgatctgc tgggacaggt
     1621 agagggcgga gggccggtgg agatcgaccg gctggcaatc gcattcgatc gaatggctga
     1681 gcagatccaa agcagggaag aaagcctgaa agaggccaac tggcttctta tggctgccac
     1741 ggtacgaaat caacagcttg ccgagcgcgc ccaggcaagc ctggcgcagt tcgaggccat
     1801 agtggaaagc atgaccgacg ggctgatcct ctccgacgcc aggggcaaca tcctccgaat
     1861 gaatcctgcg gcgctagtca ttcacgggat ggatcaactg gacagcgcat cgcttcagtt
     1921 caactccttc gaagggcttt accagatgat ggaagccccc tccggtcggc ccctgactcc
     1981 gacagagagg ccctttgcac gcgccctggc aggagagagc ttctcccagc tacaggttag
     2041 agtgcgcccc attggcaacg aaaccgagag ggcgatcagc tacaacgggg cgcctatccg
     2101 agaccgggac ggaaagatca ccatggccgt ggtgaccatg agggacgtca cggcacgacg
     2161 tcgggcagag gccgagcggg aaaagctact ggcagaattg gacgccacca tcagcacgct
     2221 catgtccgtc gccgatggcg tgctgctcta cgatcctgca ggcaagatca cccaggtgaa
     2281 cagcgtggcc caacggctgt tggcctgcgg tccgaacggc gaaggtttgc cggccggtct
     2341 ccgcgccact cctcccctca tcgtccaatc ctcgtccgca accgcccagc cgaacacgat
     2401 cccggccagg gcactcaagg gcataggcac ccacgccgag gtgatgaggc tgcagaagaa
     2461 cggctccacc acctggatgg ccgtatcagc cgcacctgtc ctggctaagg atggacagaa
     2521 gttgggcgcg gtggtcacgc ttacggatat cacggtgctc cgagaactcc aggaaagaca
     2581 agagaagctg ctacgggatg tcaaggcaag caacgagatg ctcgttcaat cctccatccg
     2641 cgagaggacg ctagccgcag aggcccagcg ccaagccgcc cagatgaacg ccctcctgga
     2701 aaaactcggg gaggcggtgg tggtgctcga cggcgcgggc aacattcttg ttcgcaacgc
     2761 aaaggcagaa gagatcgacc ctccgccggg agcaaacatc cggaacggtg ccgaattgtc
     2821 tccccgatcc attctccagg ccgacggctc catcatgccg atagagcaac tgccacagtc
     2881 tcgagtgctg cgcggtgaga gtttcgccga ttacgaggta atctacacgg aaccagggga
     2941 cactatgcgt cacctcctct tctcagcggg ttcattgcgc gatgaaacgg caaaggtggt
     3001 attggggata ctggtgtacc gtgacgtgac ggagttgaga cacctggagc aggtaagaga
     3061 cgactacatg agggccgtct cccacgatct ccgcaacccg ctgactgcta tccagggcca
     3121 cgcacaactc ttgaggaggc tactagagca ggatggagtg gacaccaaga ggcttcactt
     3181 cctggatacg atcatcgcca acgccacaag gatgaacagc atgatcaaag acctggccga
     3241 atcagccaga ctcgaagccg gccagcgcac cctggaatgt gttccgctcg acctgccggc
     3301 cttcacactg aaccttaagg agcgactgag caccccctca gatgcagagc gcatccgcgt
     3361 ggaggctcct ccggacctgc cactcgtctg ggccgacgaa gaggcgttgg agcgcatact
     3421 gaggaacctc ttgggcaacg ccctcaagta ttcaccggcg gattgccccg tcatcgtcac
     3481 catggcgtcc ggcgacaagg agatagtcac ctcggttcgc gacggtggcg agggcatcgc
     3541 gaccgaagag cagcaacatc tcttccaacg ttactatcga gcaagttcgg gaaaagccaa
     3601 caggggaggg ctcgggctgg ggctctttat cacgaagggg ctggtagagg tccacggcgg
     3661 caggatctgg gtggagagtg agatcggcaa gggctcgatc ttccatttca ccctgcccgt
     3721 tgccacgcgc tgagttgcac cctcagccat aacaaccaca tgctataatt agctggtttc
     3781 gcttgcacgt caatacgatg gccctcaggg tggtcattcc aatctagggg aggtgcagag
     3841 gtgtcgcaca acatccagtg gctcgggcac gacgtgttca agatctccag caacgggatc
     3901 gtcatctaca cggacccctt cagactccgg gcaggaggag acaaggcaga catcatcctc
     3961 gtcacccacg atcacttcga tcattactcg ccggacgaca tcgcaaagat cgtgaggcag
     4021 gataccgcaa tagttgcccc tcactccgtg gcggacaaac tcgccgggga ggcacgatcc
     4081 agcacatcca agttggatgc acagaagatt gaagaggtga ggcccgggaa tcggctggag
     4141 gtcaatggga taccgataga ggtaacctgg gcgtacaacg tcaacaagtt ccgcaaccct
     4201 ggggagccgt accacccgaa gagtccagat ttcgtcggct acatattcac cgtcgatggc
     4261 aagcggatct acctcgcggg ggatacggat atgatccctg agatgaacac catcgacgtg
     4321 gacatcgccc tgcttccggt aagcggcacc tacgtcatga cggccgacga ggccatacag
     4381 gcagccaaca gcgccgtcaa atccaagagc tacataccta tgcactacaa cgccagtcga
     4441 ggtaggtgag gattatcccg tgctcggggg agatggccgg cagttgctcc agcctgttca
     4501 actccctctc tgcctcctgc cgcgcctccg atggtagctc tgcctcctcg atccgttttc
     4561 gtatatcgct gacctctctg gcctgctcgg tttcttcgcc cagttcgcgc tggattgccc
     4621 tgagctgctc tcgcagcaga tattctcgct gactcttgcc gatctgctcc tcggctcggg
     4681 accggatgct ctccccgatc tgggtgatct cgatctctct ctgcacgtgg tcggtgagca
     4741 gc
//
LOCUS       DATALS010000152         9852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_149069, whole genome shotgun sequence.
ACCESSION   DATALS010000152 DATALS010000000
VERSION     DATALS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149069"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..899)
                     /locus_tag="VHS06_03315"
     CDS             complement(159..899)
                     /locus_tag="VHS06_03315"
                     /inference="COORDINATES: protein motif:HMM:NF044761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2987181.1"
                     /translation="MARTVNEREFTRKRNEILEAAMRLVLAKGYERMTIQDILTDLRI
                     SSGAFYHYFGSKPALLEAFIEQIGEVAEQPLLPIINDPHLSAIEKLRGFFGVLSRLVI
                     THSADVAELGPVWFTDENAIVRQKVDEATARQRVPMLAAIVRQGIQEGVFTTPYPDLA
                     GEVVHSLLQGMQNAHAGLLRLAEQDGSEAYPLEDIVTSHSAHIVTTCAAYMDAIERVL
                     GAPPNSLHRVGAEEVAAWAVAARGTYRA"
     gene            complement(1346..2980)
                     /locus_tag="VHS06_03320"
     CDS             complement(1346..2980)
                     /locus_tag="VHS06_03320"
                     /inference="COORDINATES: protein motif:HMM:NF004807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX2987182.1"
                     /translation="MQGSIAVASILKQEAVEQLFCLPINPLIEACAELRIRPYMARTE
                     RHAIGMADGFSRVTNGRRIGVCASQYGPGAENSFGGIAQVYSDSTPVLYLPAGPNQAR
                     LGVANTFDITSNFRNITKYAARINSAGRIPEFMRRAFTYMRAGRPGPVALEVPVDVMA
                     AQLDDLVYSPVERPLSMAAPSAVAAAVKAIVAAKKPIIHAGVGILYAEAWDELQEFAE
                     LVQAPVMTTVQGKSAFREDHPLSLGAAAQAGSMALARYLAEADLIFGAGSGLATSAYD
                     APIPKGRVAVQLTVDERDFNSDYAVQYMLQGDAKLVLQQMIDEARRQLGKDGRKGDGK
                     VAAAIKAIKDAWMAEYMPKLTDNETPMNPYRVIWEVQHAVDLRNTVATHDAGSPRDMM
                     VPFWPALEPNSYIGWGKSTHLGYGLPMAMGVKVAKPEKTVLNVMGDLAFGMSGLELET
                     AARNHIGTITILFNNGLLGGYDKHLHVSTELYGTRYITGNYSKIAEGLGAYSERVEKP
                     EEIAGAVKRAMKANGDGKPALLEMMTREETKLSKYW"
     gene            complement(3778..4041)
                     /locus_tag="VHS06_03325"
     CDS             complement(3778..4041)
                     /locus_tag="VHS06_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987183.1"
                     /translation="MIILQIRLRCIILAAFVMAGVGDQRGFLRVLYNHGVMAVVNGAA
                     EVSCAEKNEAGDRLGRRPHRFTAIYEIGERSGLGPSYLVSIPA"
     gene            4123..4968
                     /locus_tag="VHS06_03330"
     CDS             4123..4968
                     /locus_tag="VHS06_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX2987184.1"
                     /translation="MIPKAETSRDPIDGIIASLSDGEEVTLATVVGPRGLPAEFLGRK
                     LLYLTDGRVEGSIGAWPVDLRVARECAIHRQNGQTSVLLPIELSSDECQDLGLAPGLK
                     LELFIDLLTPSPSLLIIGGGHIAQPLCTVAQALGFSVTVMDDRASFANCDRFPEADDI
                     VVGYFEEELARYPIHSSTYIIIVTRGHAYDQASLRAVIGSRAAYIGMIGSAHKVRTVM
                     SNLAEAGVPKEQMEKVYAPIGLDIGAETPAEIAVGIMAEIVNVRRRRKRHSASLSQRP
                     LSRRP"
     gene            5003..5326
                     /locus_tag="VHS06_03335"
     CDS             5003..5326
                     /locus_tag="VHS06_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX2987185.1"
                     /translation="MLDLFREIARRQDQGETVAMATIVSSKGSTPRGVGARMMVAADG
                     SIAGTIGGGCGEHQVCLAALDVIRSGNPKMVHVELTNEVASNEGSACGGVMDVFVEPI
                     AGRAG"
     gene            complement(5353..7188)
                     /locus_tag="VHS06_03340"
     CDS             complement(5353..7188)
                     /locus_tag="VHS06_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2987186.1"
                     /translation="MVYVSALRPSVLLEALQEEHPNAIIAVPRLLELLRDRALQDVGN
                     AAIGRKLFERLLAAAPSLSLDVRRKLFWPVHRQLGGRLKYVVSGGAELDPEVERFWDA
                     LGVVALQGYGLTETSSAVSCNRVEDRRLGSVGKPLGNQDVMLATDGEILVRGANVMPG
                     YYGDSGATAEAFEDGWFRTGDIGIRDRDGYLYIRGRKKEVIVTSAGVNVYPDDVEAVI
                     DRVAGVRASAVVEWQGGVYAVLLLGNGIDPRTVVREANRQLDPSQQILGYTLWPGLDF
                     PRTATMKVRRASVLDYLRQSSSGVVAPATARGGIVTPLRQLVAEVAHRPPSAVAGGQE
                     LATDLGLGSIDQVELVSRIEQELGVDVPEDALTPETTVAELELLVGRERRRTGQVVFS
                     GWSISPEMSGARDVIQRRLLFPALRQVVSLEVRGLENLAGLSGPHLLAANHTSLLDTP
                     TILMALPPELRQRTAAAVWAEFFEVPDRPWWYRAWRRLEFYLANAGIAVLPLPHSRIV
                     RPSMRWAGRLADRGWNLLIFPEGAETRTGEIEPFKEGVGVMVSWLHLPVVPVRVEGLF
                     AVLPHETLWPRPGRASVRFGRPLSFPSTASYLEITRQIEDAVRGL"
     gene            complement(7176..7835)
                     /locus_tag="VHS06_03345"
     CDS             complement(7176..7835)
                     /locus_tag="VHS06_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2987187.1"
                     /translation="MTLADLVVDMERLGDRELFRHRGEFRCYSWSYQRVATEARRFAA
                     YLQTRGVERGDRVMIWAPNSPEWVVVFLGCLLAGAVPVPIDLRSRGQFLRNVQSQTAA
                     RLLFTTRFRPDPEIGIPAVYLEGFGEALRDARPEQFRPVSTAEDDLAEILYTSGTTGN
                     PKGVMLTHRNLLSELEAFQPIVPPEPYYRFLSVLPLSHILEQMGALLLPISRGGDRWC
                     T"
     gene            complement(7822..8190)
                     /locus_tag="VHS06_03350"
     CDS             complement(7822..8190)
                     /locus_tag="VHS06_03350"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX2987188.1"
                     /translation="MIVKIGIDLVHIPRMRELVEREAFLARTFHPSELRDRRPMRLAG
                     IFAAKEALFKALGAPHQWLEVEVDHDPEGRPFLRLSPASAPPEILSLDLSVTHEGEYA
                     VAVVVALLEGESAEANDDAG"
     gene            8402..9094
                     /locus_tag="VHS06_03355"
     CDS             8402..9094
                     /locus_tag="VHS06_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX2987189.1"
                     /translation="METVVLDGIQARLPREELMRKLHVGEDSPQAVELLELIDQAEAI
                     GRPKGLCGVAFVDDRGDDFVVIDGERFSSRVLAVNLSPVNRVFPYLATCGTELEEWAN
                     GLDDMLLRYWAEAIRESAMRCAMAETERFVKESYAQDETSRMSPGSLEDWPIQQQVPL
                     FRLLGETESTIGVRLTESLLMVPTKTVSGIRFGAESGFESCMLCPRPVCQNRRAPYDA
                     ALFDSRYRKSAD"
     gene            9314..>9852
                     /locus_tag="VHS06_03360"
     CDS             9314..>9852
                     /locus_tag="VHS06_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2987190.1"
                     /translation="MEDTNRSMKGKLCVVTGATSGIGRETALGLARLGAEVVVVGRDR
                     TKTEGVTKEIREETGNSAVHYALADLSSQTEIRRLAQELKDRFPRLDVLVNNAGALFM
                     SRQETVDGIEMSLAVNYLGAFLLTNLLLDRLTATAPARIVNVAAEAHRQAKLDLDDLT
                     WSKKRYHGQPAYARAKLALL"
BASE COUNT         1974 a         3145 c         2981 g         1752 t
ORIGIN      
        1 ttcacaggat ttggctacag ccctcatttc cgggaagctg cacttcccat caagtattac
       61 aaacaccgtg gagagaacga ttgtgacatg gcgcggggcg gagcgctccg acactccggt
      121 tcgacaattc accttctcct gagccgcatt cggcctccct acgcccggta ggtacctcgc
      181 gccgccaccg cccatgccgc cacctcttca gcgccgacac gatgcaggga gttcgggggc
      241 gcgcccaata cccgctcgat ggcatccatg taggcagcac aggttgtgac gatgtgggca
      301 gagtgggatg tgacgatatc ctcgaggggg tacgcctcgc tgccatcctg ctccgccaaa
      361 cgcaacaacc cggcatgcgc gttctgcatg ccctgcaaga gggagtggac gacttcgccg
      421 gccaggtcgg gatagggggt cgtgaagacg ccttcctgaa tgccctggcg aacgatggca
      481 gccaacatcg gcacacgctg cctggcagtc gcctcatcca ccttctggcg gacgatggca
      541 ttttcgtcgg tgaaccaaac cggtcccagt tccgccacat ctgcgctgtg ggtaatcacc
      601 aaacggctga gcactccaaa gaagccccgg agcttctcga tggcggatag atggggatca
      661 ttgatgatgg gaaggagcgg ctgttccgcc acctctccga tctgctcgat gaatgcctcc
      721 aggagggccg gcttcgaccc gaagtagtga tagaacgctc cactggagat ccgcagatcg
      781 gtgaggatgt cctggatagt catgcgctcg tagcctttgg caaggacaag ccgcatcgcc
      841 gcctcgagga tttcattgcg ctttcgggtg aattcccgtt cgttaacagt ccgtgccatc
      901 tgtatcgcta gcgattaata aaccgagcct ctatttattt cccgcggcag tatcctgcag
      961 gtatgccccg gtgtcaagtg gctgtcagaa ggatgccggc aaccgagtcc gccctgcgca
     1021 ggaagtcgta ccaggtacca gcgtcgaggt cgggcaagga gtatgccatc ttcgatggca
     1081 gtacggctat acaggctgac acagagtagt agcggctccc gtagaatgca gccgccgcag
     1141 aaatcgattt cggaacacgt tttcactggc aggggatgcg aggcgatggc aaacggagaa
     1201 gtctaacgag gcacaccatc cgatagggcg accgtgtgcc ctagacatcg aggagccggg
     1261 gtcgtccccg accccggctc ctcacgatgc tgagagcctt gacacggggc gggtcaggga
     1321 cgacccgccc ctccaatgca aggatctacc agtacttgga gagcttggtc tcctcgcgag
     1381 tcatcatctc cagcaaggca ggcttgccgt cgccgttcgc cttcatggct cgcttcaccg
     1441 cgccggcgat ctcctcaggc ttctccaccc gctcggagta ggcaccgagg ccttcggcga
     1501 tcttgctgta gttgccggtg atgtagcggg tcccgtacaa ctccgtggat acgtgaagat
     1561 gcttgtcgta cccgcccagc aggccgttgt tgaagaggat ggtgatggtg ccgatgtggt
     1621 ttctggctgc cgtctccaac tccaaaccgc tcatcccgaa cgccagatcg cccatcacgt
     1681 tcagcaccgt cttctccggc ttggcgacct tgacgcccat cgccatcggg agaccgtaac
     1741 cgaggtgagt ggacttgccc cagccgatgt aggagttggg ctccagcgcc ggccagaatg
     1801 gaaccatcat gtcacgtggg ctgccggcat cgtgggtagc cacggtgttc cgcagatcca
     1861 cggcatgctg cacttcccag atgaccctgt acgggttcat gggggtctcg ttatcggtca
     1921 gtttcggcat gtactcggcc atccaggcat ccttgatcgc cttgatggcc gccgcaacct
     1981 tgccgtcgcc cttgcgcccg tccttgccca gctgccgccg agcctcatcg atcatctgct
     2041 gcagcaccag cttggcatca ccctgcagca tgtactggac ggcgtagtca ctgttgaagt
     2101 ctcgctcgtc gacggtcagc tgcaccgcga ccctcccctt cgggataggc gcatcgtagg
     2161 cagaggtggc caggccgctg cccgcgccaa agatcaggtc cgcctcggcg aggtagcgcg
     2221 ccagcgccat tgacccggcc tgcgcggcag caccaagaga taggggatga tcctcgcgga
     2281 aggcgctctt gccctggacc gtagtcatca ctggagcctg aaccagctcg gcaaactcct
     2341 gaagctcgtc ccacgcctcg gcgtacagga tcccgacgcc cgcgtgaatg atcggcttct
     2401 tggcggcaac gatcgccttg acggccgcgg ctacggccga cggggcagcc atggagagag
     2461 ggcgctcgac cggggagtag acgaggtcgt ccagttgggc cgccatgacg tccacgggca
     2521 cttctagcgc aacagggccg ggtctgcccg ctcgcatgta ggtgaacgcg cgccgcatga
     2581 actcagggat gcggccggca ctgttgatcc tggccgcata cttggtaatg tttcggaagt
     2641 tgctcgtgat gtcgaaggtg ttggccacac ctagccgcgc ctggttcggg cccgcaggaa
     2701 gatagagaac cggcgtcgag tctgaataga cctgggcgat cccaccaaag gagttctcag
     2761 cgccaggacc gtactgggag gcacaaacgc cgatgcgccg cccgttggtc acgcgagaga
     2821 agccgtcggc catgccgata gcgtgccgct cggtgcgagc catatagggc ctgatacgca
     2881 gctcagcaca ggcttcgatc aaggggttga tgggcaggca gaacagctgc tcaacagctt
     2941 cctgcttcag gattgaggca accgcgattg aaccctgcaa gtgcagtcct ccagcggcgg
     3001 cgagggtccc ggtgttgagc gtatgcatcg gcgactgttg acatcgaagt cacgacggca
     3061 cagcttttct gccgcactca gagcaccgtg agaacaagaa ggttcaaatg gagcgaaaag
     3121 cgggggctgt cagaacactc gaaagtgggg acgtgatcgg gacgtaatac tgaatagggg
     3181 ccagcgcctc cattgccgcc tcaccagcga acccaagcag acagcatcgc cacggttaca
     3241 ccatcataac cttgctcgcc atcggtagat gcttgctgag accctcacca gattttatgt
     3301 cgtggcgggc aaccgcatca tcgctgctgc gcggccacag ctacatgaaa tagcacgaat
     3361 gcccataaca aatcaagtcg aggcggaggc agcctgtact taacagctat ttaacgatcg
     3421 tcaaacaacc tcttaaccac attccccttt ccctggtcta tactcccccg tgtacagacg
     3481 cttcaatcct cctactcatc ctggcagggc gggtcgctct tgacgggaag agcggcccgc
     3541 cggatcccag tagcgattcg caagctgtac gggtagttga cgactcagga ggcaagtgat
     3601 gaacgcacta ggacaaagga ggccaccggt acccggcaag tatcaggaat cttcgaggct
     3661 gcaggtttgg tacaggcacg gttacagcca ggccgccagg ggctttaccc ccacggaaaa
     3721 ctggctgaac tgcttgaggc aggacaaagc cgtgtcggag gcctattacg aaggttatca
     3781 agcggggatg ctcaccaaat aggacgggcc caaccccgag cgctccccga tttcatatat
     3841 cgccgtgaaa cggtgaggtc ggcgaccgag acggtcaccg gcctcgtttt tttcggcgca
     3901 gctcacctca gcggcaccgt tcactacggc cattacccca tggttgtaca gcacccgaag
     3961 gaagccccgt tgatcgccaa ccccggccat cacgaaagcg gccaggatta tgcagcgtag
     4021 cctgatctga aggatgatca actgcgcgtt gaggtgctac aatgaagttc gatttatgcc
     4081 tgttgtactg ctacaatagg tactatgcgg ggaggcgcag ccatgatccc caaggcagaa
     4141 acttcccgtg atcccatcga cggcatcatc gcctcactga gtgacggcga agaggtcacc
     4201 ctggcaaccg tagttgggcc cagaggcttg cctgccgagt tcctcggccg aaagctgctc
     4261 taccttaccg acggcagggt ggaggggagc atcggggcgt ggccggtaga cctgcgagtc
     4321 gcccgggaat gcgccataca tcgacagaac ggccagacgt cggtactgct gccgatcgag
     4381 ttgtcgtccg acgagtgcca agacttgggg ttggctccag gcttgaagct agagctgttc
     4441 atcgacctcc tgaccccgtc gccgtcgctg ttgatcatcg gagggggtca catcgcccaa
     4501 cccctctgca cagtcgccca ggccctcggc ttcagcgtga cggtgatgga cgaccgagcg
     4561 tccttcgcca actgtgatcg cttccccgag gcagatgaca tcgtggtcgg ttacttcgag
     4621 gaggaactcg cccgctaccc gatacacagc tccacataca tcatcatagt cacccgtggg
     4681 cacgcctacg atcaggcgtc tctacgtgca gtcatcggat cgcgcgcggc gtacatcggt
     4741 atgatcggca gcgcccacaa ggtgcgaacc gtgatgagca acctggcaga agcgggtgtg
     4801 ccgaaggagc agatggagaa ggtctacgct ccaatagggc tggacatcgg ggccgagact
     4861 ccggcggaga tcgcggtcgg gatcatggct gaaatcgtca acgtccggag aaggcggaaa
     4921 cgacattcgg cgtcgctgtc gcagcgtccg ctatccagaa gaccataacg aactggggca
     4981 tgccgtcgaa aggtgaattc ggatgctaga tctgtttcga gaaatcgcgc ggcgacagga
     5041 tcagggggag acggtggcca tggccaccat cgtaagcagc aaaggatcta ctccgcgagg
     5101 tgtgggcgcc aggatgatgg tggctgccga cggctccatc gccggcacca tcggcggagg
     5161 ttgcggtgag caccaggtct gcctggcggc gctggacgtc ataaggtctg gaaacccgaa
     5221 gatggtccac gtcgagttga ccaatgaagt ggcatccaac gaaggctccg cctgtggggg
     5281 agtgatggac gtcttcgtcg agccgatagc cggacgagcc ggatgatggc gtctcggcta
     5341 ccgatggcgt gatcacagcc cccgcaccgc atcctcgatc tgccgggtga tctccaggta
     5401 ggaggcagtc gacgggaagg agaggggacg gccgaagcgc accgaagccc tccccggccg
     5461 cggccacaac gtctcgtgcg gcagcactgc aaacagcccc tccaccctca cggggacgac
     5521 cgggaggtga agccacgata ccatcacgcc caccccctct ttgaacggct cgatctcacc
     5581 cgtccgcgtc tccgctccct cggggaagat aagcaggttc cacccacggt ccgccaatct
     5641 gcccgcccag cgcatgctgg ggcgaacgat cctggagtgg ggcaacggaa gcaccgcgat
     5701 tccggcgttg gccaggtaga actccagccg cctccacgca cggtaccacc agggccggtc
     5761 aggaacttcg aaaaactccg cccagaccgc cgcggcggtg cgctggcgca attccggagg
     5821 cagcgccatc aggattgtcg gggtgtccag aaggctggtg tggttggccg ccagcaggtg
     5881 tggtccggag agtccggcga gattctctag ccccctcacc tctagcgaga ccacctgtcg
     5941 aagggcagga aacagcaacc ggcgctggat cacatccctc gccccactca tctcggggga
     6001 gatcgaccaa cccgaaaaga ccacctgtcc ggtgcgtctc cgctccctcc ctaccagcag
     6061 ctccagctcc gcaaccgtcg tctctggcgt caacgcatct tcgggaacgt ccacccccaa
     6121 ctcctgctct atgcgagaca cgagctccac ctggtcgatg gagccaaggc ccaggtccgt
     6181 tgccaactcc tgcccccccg ccacagcaga gggcggtcgg tgggccacct ccgcgaccaa
     6241 ctgccgcagt ggggtaacga tccctcctct ggcagttgcg ggggccacaa ccccgctgct
     6301 ggactgccgg aggtagtcca gaacgctcgc cctgcgcacc ttcatcgtgg ccgtgcgagg
     6361 aaagtccagt cccggccaga gggtgtaccc caggatctgc tggctgggat cgagctgcct
     6421 gttcgcctcc ctcacgactg tccgagggtc gatcccattc cccagcagca acaccgcgta
     6481 caccccgccc tgccactcca cgactgcgct ggccctcacc cctgcaaccc ggtcgatcac
     6541 tgcttccacg tcgtcggggt agacgttgac ccccgcggag gtgacgatca cctccttctt
     6601 gcgccccctt atgtacagat atccgtcccg atcacgtatg ccgatgtcgc cggtccggaa
     6661 ccaaccatcc tcgaatgcct cagcagtagc ccctgagtca ccgtagtagc caggcatgac
     6721 gttcgccccc ctgaccagga tctcaccgtc ggtggccagc atcacatcct gattccctag
     6781 tggcttcccc accgatccaa gtctgcgatc ctccacccgg ttgcagctga ccgccgagga
     6841 ggtctcggtg agcccgtagc cctgcagcgc caccacccct agcgcgtccc agaagcgctc
     6901 gacctctggg tccaactccg ccccgcccga cactacatac ttgagccgcc cccctagctg
     6961 ccggtgcacc ggccagaaca gctttctgcg aacgtcgaga ctgaggctgg gcgctgccgc
     7021 cagcaaccgc tcgaacagct tgcggcctat tgcagcattt cccacatcct ggagcgccct
     7081 gtcgcgcagc aactccagca gccgggggac cgcgatgatg gcgttgggat gctcctcctg
     7141 caaagcctcc agcaacaccg atggccgcaa tgcacttacg tacaccaccg atcccccccc
     7201 cgagagatgg gcagcagcag tgcccccatc tgctccagga tgtggctgag gggcagcacc
     7261 gacagaaagc ggtagtaggg ctcgggcgga acgatcggct gaaatgcctc caactccgaa
     7321 agcaggttgc ggtgggtgag catgaccccc ttcgggttgc cggtggttcc ggaggtgtac
     7381 agtatctcgg ccaggtcgtc ttccgccgtg gagactggcc ggaactgctc gggtcgtgcg
     7441 tcgcgcagcg cctcgccgaa gccctccagg tagacggctg ggatgccgat ctcgggatcg
     7501 ggcctaaagc gggtggtgaa gagcagtcga gccgccgtct gagactgcac gtttcggagg
     7561 aactgaccgc gagagcgaag gtcgatcggc accggcacgg caccagccag gagacaacct
     7621 aggaacacca cgacccactc cggactgttg ggtgcccaga tcatgactcg gtcgccacgc
     7681 tcgacgcccc gcgtctgaag gtaggccgca aagcgcctcg cctcggtcgc taccctctgg
     7741 tagctccagc tgtaacagcg aaattcgcct ctgtggcgga acaactccct gtcgcccagc
     7801 cgttccatgt cgacgacgag gtcagccagc gtcatcgttc gcctcggcgg actcgccctc
     7861 cagcagtgct acgacgaccg ccaccgcata ctcgccctcg tgggtcacgg agaggtccag
     7921 cgacaggatc tcaggaggag ccgacgcggg agacagtcgc aagaacggcc tgccctcggg
     7981 atcatgatcg acctccacct ccaaccattg atgaggggca ccgagcgcct tgaacagcgc
     8041 ctccttggca gcgaagatcc ccgccaatct catcggacgc cggtctcgca actctgatgg
     8101 atggaacgtc ctcgctagaa aggcttccct ctccaccaac tccctcatcc tgggtatgtg
     8161 cactagatcg atgccaatct tcactatcat cgcgattccc ccgcgtagcg gcggcaacat
     8221 ccgtgccggt gagagatgga gacggcgacc gcgagtgcta agcggctttc cctggatgcg
     8281 gtcaaccttt ctgtgaggga aaagcggaca ccaacaaggg aaagtcgctc aggtcctcct
     8341 ttcaggtaca atgtcttcca cgggccttcc cggcccaggt tggataatcg aggtgtcgac
     8401 catggagacg gtggttcttg atggcatcca ggcacgactg ccccgagaag agctgatgcg
     8461 aaagctgcac gtgggtgagg actccccgca ggcagtcgag cttctggaac tgatcgacca
     8521 ggcggaggca atcggaaggc cgaaggggct gtgtggagta gccttcgtcg acgatcgggg
     8581 tgacgatttc gtggtgatcg atggggagag gttctccagc cgagtcctgg cagtgaacct
     8641 ttcgcccgtc aacagggtgt tcccctacct ggccacctgc ggcaccgaac tggaggagtg
     8701 ggccaacggc ctggacgata tgcttctccg ctactgggcc gaggccatcc gggaatccgc
     8761 catgcgctgc gccatggccg agacagagcg attcgttaaa gaaagctacg ctcaagacga
     8821 gacgtcgcgc atgagtccag gctcgctgga ggattggcct atccagcagc aggtcccgct
     8881 gttccggctg ctgggcgaga cggagagcac catcggggtt cgattgaccg agagtctgct
     8941 gatggtcccg accaagaccg tgtcgggcat ccgcttcggc gccgagagcg gcttcgagag
     9001 ctgcatgctc tgccccagac cagtctgcca gaaccgtcga gcaccctacg atgcggccct
     9061 cttcgacagc cgctatcgca agagcgcaga ctgacccgat ccaccctgtc gcgcgttcca
     9121 agcggtgcaa tctggtaaac tcccgacgcc ggtatacggc attgggaagg ccccgcgtga
     9181 gccctgcaag gtgaggcagg tcagcctaga ggctgctgat gcgtacaggc ggggccgcac
     9241 tccaaattgt cgactctggc gtcgagccca cgcgctccaa attgccggca tcatgaacgg
     9301 aggacacacc accttggaag atacgaaccg gtctatgaag ggaaaactgt gcgtcgtaac
     9361 aggggcgacg tcgggcatag gacgggagac ggccctggga ttggcgcgac tcggtgccga
     9421 ggtggtggtg gttggccgcg accgaacaaa gaccgagggt gtaacgaagg aaatccggga
     9481 agaaacgggg aactccgccg tccactatgc cctggccgac ctttcgtcgc agactgagat
     9541 tcgccgtctc gcccaggaac tgaaagatcg cttcccgcgg ctggacgtcc tggtgaacaa
     9601 cgccggcgcc ctcttcatgt cgcggcagga aaccgtggac ggcatcgaga tgagcctggc
     9661 ggtcaactac ctcggcgcct tcctgttgac gaacctgctc ctcgaccggc tcaccgcaac
     9721 cgcccccgcg aggatcgtca acgtcgcggc agaggcccac cggcaggcca agcttgacct
     9781 ggacgatctg acgtggagca agaagaggta tcacgggcag cccgcatacg ccagggctaa
     9841 actggcgctg ct
//
LOCUS       DATALS010000153         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_372310, whole genome shotgun sequence.
ACCESSION   DATALS010000153 DATALS010000000
VERSION     DATALS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372310"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VHS06_03365"
     CDS             <1..299
                     /locus_tag="VHS06_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987191.1"
                     /translation="GGGAMSGKEVSAEALKRLREERAGQLPAIQARLKEQVKATNEIT
                     RLLKERPRTVPELAEAAGIPTQAAFWYLMGLKKYGRVAEGEQDGDYFQYRLVGE"
     gene            302..1480
                     /locus_tag="VHS06_03370"
     CDS             302..1480
                     /locus_tag="VHS06_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2987192.1"
                     /translation="MTVHRLDTTLARDLKKFGAFDIDACFNCGNCTAVCNHSSETALF
                     PRRLIRFGQLGMRDRLVSSREAWLCWNCRDCSDTCPRQARPSEYMESVRRYAIADLDP
                     TGISRRMYTSPLFVVGFALFLAVLFAGVLLSRAGEPAAGFLDLFGFIPFELIHDLGVV
                     VMAAIGLVAVINTVRLVRLLSRSLEPTVSGEEAVSGSAGRLIAAVGAVIREMVGQRRF
                     RACEVDSDKPFFLRPWFVHYTIMWGFIGLGVATALDFMFKTPGEVVPLWYPARMLGTA
                     AGLALMYGSAITLARKLRPLDRTQPRMGPSDWLFLGLLFMVGLTGFALEAMVYLPRGS
                     ALGYGIFLTHVVLAMELLVLFPFSKFAHALYRPLAYGIYWYKTAPSRDETAGEYAAAD
                     "
BASE COUNT          252 a          451 c          566 g          331 t
ORIGIN      
        1 gtggaggtgg cgcgatgagc gggaaagagg tgtccgctga ggcgctgaag cgtcttcggg
       61 aggaacgggc cgggcagttg ccggcgattc aggctcgact gaaggagcag gtaaaggcga
      121 ccaacgagat cacccggttg ctgaaagagc ggcccaggac cgtgccggag ttggccgagg
      181 cggccgggat accgactcag gccgccttct ggtacctgat ggggctgaag aagtacggca
      241 gggtcgccga gggcgagcag gacggcgact acttccagta tcgactggtg ggtgagtgaa
      301 catgacggtg catcgactgg acactaccct ggcgcgcgat ttgaagaagt tcggcgcctt
      361 cgatatcgac gcctgtttca actgcggtaa ctgcacggcc gtctgcaacc atagctctga
      421 gacggccctt tttccccgca ggctgatccg cttcggccag ctggggatgc gggataggct
      481 ggtttcctcg cgagaggcat ggctttgctg gaactgccgg gactgctccg acacctgccc
      541 tcggcaggct aggccgagcg agtatatgga gtcggtgagg cggtacgcca tcgccgacct
      601 cgatcccacg gggatctcca ggcgaatgta cacctcgccc ctcttcgtgg tggggtttgc
      661 gctcttcctc gcggtactgt tcgcgggggt gcttttgagc cgggcgggcg agcccgcggc
      721 cggcttcctg gatctgtttg gcttcatacc cttcgagttg atccacgacc tgggcgtggt
      781 tgtgatggcg gccatcgggc tggtggccgt gatcaacacc gtgcggctgg ttcggcttct
      841 gtcccgatct ctggaaccca cggtttctgg cgaggaggca gtgtcgggat cggcgggcag
      901 gttgattgcc gcggtcggcg cggtgatccg agagatggtt gggcagaggc gcttccgcgc
      961 ctgcgaggtg gattccgaca agcccttctt cctgagaccc tggtttgtcc actacacgat
     1021 catgtggggt ttcatcgggt tgggcgtggc cacggctctc gacttcatgt tcaagacacc
     1081 cggggaagtc gttcctctct ggtatcccgc caggatgttg gggacggcgg ccgggctggc
     1141 gctgatgtat ggatcggcta tcacccttgc caggaagctg cggccgttgg atcggacgca
     1201 gcctcggatg gggccatccg attggctctt tctcgggctg ttgttcatgg tggggctgac
     1261 cggcttcgca ctggaggcga tggtatacct tccgcgcggc agcgcgctgg gctatggcat
     1321 cttcctgacc cacgtggtac tggcgatgga gctgttggtg ctgttcccct tcagcaagtt
     1381 cgcccacgcg ctgtacaggc cgctggccta tgggatctac tggtacaaga cggcgccatc
     1441 cagggatgag acggcgggcg aatacgccgc cgcggattag tcaactgaca cgctggattt
     1501 gaccgctagg agcccgggtc gtccctgacc cgggcggtgg cgtgagaggc cgtcggggtc
     1561 agggacgacc ccgactccta gtgtcaagat cagcgtgtca
//
LOCUS       DATALS010000154         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_15194,
            whole genome shotgun sequence.
ACCESSION   DATALS010000154 DATALS010000000
VERSION     DATALS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15194"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VHS06_03375"
     CDS             complement(<1..522)
                     /locus_tag="VHS06_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2987193.1"
                     /translation="MAPGRLVRELMNLIGDGYVLWEPYDLRLYEYDGSIDRALPQAIV
                     LPEGTVQVAAVVRACNRAEVPFTARGGGTGLSGGAIPVEGGVVISLAKMNRVLEVDLD
                     NLRAVVEPGLVNLHLSQAISEHGLYYVPDPSSQKACTIGGNVGENAGGPHTLLYGVTT
                     NHVLGVEVVMPDGE"
     gene            complement(882..1478)
                     /locus_tag="VHS06_03380"
     CDS             complement(882..1478)
                     /locus_tag="VHS06_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX2987194.1"
                     /translation="MLREFQIVGDDLFRAGLTSSHGGNLSVRMGDRVVITRRGSMLAH
                     LEEHDLIETGVWENDSGVMLASSELVVHRAIYQNSWALAVCHCHPPHAVALSLIQDEI
                     IPVDSEASYMLHKVPVYGAETTIGSTEVAEILSKALKEYKIVMLRGHGCFAIGHMLEE
                     AYQWCSTLEAAATIICHLRALGGDVKDYRKGADRFTCW"
     gene            complement(1472..>1835)
                     /locus_tag="VHS06_03385"
     CDS             complement(1472..>1835)
                     /locus_tag="VHS06_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2987195.1"
                     /translation="LVVCKFGHWALSEEHYARLVSAATGRRYEEAELLQVGERIWNLE
                     RLINMARGFGRKDDTLPERVLREGAPSGPAKGRVVDLEPMLAEYYRFRGWSRDGAPSE
                     RKLADLGLEAYGKRVQPC"
BASE COUNT          349 a          650 c          515 g          321 t
ORIGIN      
        1 ctcaccgtcc ggcatcacaa cctcgacccc caggacgtgg ttggtcgtca ccccatacag
       61 gagggtgtgg ggacccccgg cgttctcgcc gacgtttcca ccgatggtgc acgccttctg
      121 gctggagggg tccggcacgt agtagaggcc gtgctccgag atcgcctgac tcaggtggag
      181 gttaaccaaa ccgggctcca ccactgccct caggttgtcc agatcgacct ccagcacccg
      241 gttcattttg gcaagggaga tcaccacacc cccctcaacc gggatggccc ctcccgacag
      301 gccggttccc ccaccccgcg cagtgaacgg cacctctgcc cgattgcacg ccctcaccac
      361 agcagcgacc tgaacggtgc cctccggaag cacgatggcc tgtgggagtg ccctgtctat
      421 cgacccgtcg tactcgtaca gacggaggtc gtacggctcc cacagcacat atccgtcgcc
      481 gatgagattc attaactcgc ggaccagacg tcctggggcc atgtcacact ccctaacagg
      541 cggtcctgca cctgcaacac gcgctcggtt atgtcaatcc aagctgtact atgcaggctg
      601 cggtcgacgc atagatgtgg ggatccggca acatcttcag caagcttcgt gccggaagcg
      661 ataggcagaa acgggggatc gagcacgggg gaaaatctac tcagctagtg gcaacaccga
      721 ggacgagtcg aagggcgggg tcttgtgccc cgcctagagc gtggctggaa tcaccatggc
      781 ggtcgcgccc ccggtgtggg aggcggggca ctagaccccg accttcgatc aggatgacgc
      841 gacccacaac cgatgggtag accgaaacaa cgtgcagttc gctaccaaca ggtgaacctg
      901 tccgcgccct tgcggtagtc cttgacgtca ccgcccaggg ctcgcaggtg gcagatgatc
      961 gtcgccgctg cctccaacgt ggagcaccac tggtaggcct cctcgagcat atgcccgatg
     1021 gcaaaacaac catggccgcg cagcatcacg atcttgtact ccttcagtgc cttggagagg
     1081 atctccgcca cctccgtgga gccgatggtg gtctcagcgc cgtacaccgg caccttgtgc
     1141 agcatgtagg aggcctcgga gtccacgggt atgatctcat cctggatcag gctgagcgcc
     1201 actgcatgag gcgggtggca atggcagacg gccagtgccc agctgttctg gtagatcgcc
     1261 cgatgcacca ccaactcgct ggacgcgagc atgaccccgc tgtcgttctc ccacacaccc
     1321 gtctctatca ggtcgtgctc ctccaggtgc gccagcatcg aaccgcgccg ggtaatcacc
     1381 acccgatcgc ccattcgcac actcaggttg ccgccgtgcg aactggtgag cccggccctg
     1441 aacaggtcat cccctactat ctgaaactcc ctcagcatgg ctgcaccctc tttccgtagg
     1501 cctccagccc caggtcggct agcttcctct cggacggagc cccatccctg ctccatcccc
     1561 tgaagcgata gtactcggca agcatcggct ccagatccac aaccctgccc ttagcagggc
     1621 cagagggcgc gccctcccga agcactcgct cggggagcgt gtcgtccttc ctgccgaaac
     1681 cgcgagccat gttgatcagc cgctccaggt tccagatccg ctcgcccacc tgaagcagct
     1741 cggcctcctc gtaccgccgc cccgtcgccg ccgacaccag ccgcgcgtag tgctcctccg
     1801 acagtgccca gtggccgaac ttacacacca ccagg
//
LOCUS       DATALS010000155         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_51910,
            whole genome shotgun sequence.
ACCESSION   DATALS010000155 DATALS010000000
VERSION     DATALS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51910"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VHS06_03390"
     CDS             <1..332
                     /locus_tag="VHS06_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987196.1"
                     /translation="FGHSWAVTSRWESRRKKDIRDTRSVGRTFRIVGISPEPLSSSLS
                     PSRTLHATYRRRYRHVALSLQGAALPFFRFYNSIPTSARKRKSDMECGGKPPHSRRST
                     YSLTPYR"
     gene            complement(323..>1834)
                     /locus_tag="VHS06_03395"
     CDS             complement(323..>1834)
                     /locus_tag="VHS06_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2987197.1"
                     /translation="DRCAVATMDSDRGVLVSRAACDCYGESAMGEGIEMTATQYPGSY
                     RAVASRHPVLMTPGDPSLSPLESRMLVRMGMSTAVVAPMAVGDRAIGVIFFGRRPECP
                     GFSDDDVALAGAFAGTVAVALQNATLFQEVQAQKSRTGALLASMSEGVCAVDVNMRIT
                     DVNPWLEAMVGYRADQLVGSMCGEVMCHTDDEGNRLCSTSCPLRTALQEGTGTDPITV
                     FVQTAWGELLPVLVSAAPIRDELMQVVGAVSVWRDVSREWQMDRLRTNIISVVSHEFR
                     TPLTSIIGFSEFLLNREATKEERRSALELIYKEGLRMEALVNDFLDVSRLDAGRTVLN
                     PEPLEPAAVVDRAIKAYLVRRGQDRLRGDVQPDLPKVSADPDRLDQILDNLISNAAKY
                     SAEDTQITLHAWGASYSGEDCLSIDTSGSPEWVVFTVEDRGFGIPSDQLVEIFRPFHR
                     VEGEMTRRIRGTGLGLSIVKSLVELHGGKIWVDSEVGVGSRFHVALKAISDQR"
BASE COUNT          382 a          632 c          487 g          333 t
ORIGIN      
        1 ccttcggcca cagctgggca gtgacctctc gatgggaatc ccggcgaaag aaggatatca
       61 gggatacaag gtcggtgggg cggacatttc gtatcgtcgg catatctccg gagccgctat
      121 ccagcagtct gtcgccctcc agaacgcttc atgccaccta ccgcagaaga taccggcatg
      181 tagcactctc tctacaagga gccgccctcc ccttctttcg attctacaac tccataccca
      241 catccgcaag gaagcggaaa agcgatatgg agtgcggcgg caagccgccg cactccagaa
      301 ggtccaccta ctcgctgact ccttatcgct gatcgctgat cgcctttagc gccacgtgga
      361 accgactgcc gacaccaact tcgctgtcca cccagatctt cccaccatgc aactccacga
      421 ggctcttgac gatggacagt cccagccccg tgccacgaat ccttctcgtc atctcgccct
      481 ccacccggtg gaacggcctg aagatctcca ccagttggtc ggaagggatg ccgaagccgc
      541 gatcctccac cgtaaacacg acccattccg gggagccact cgtatcgatg ctaaggcagt
      601 cttctccact atacgaggcc ccccaggcat ggagggtgat ctgggtatcc tcagccgaat
      661 acttggcggc gttggagatc agattatcca ggatctgatc cagtctgtcg ggatctgcgg
      721 agactttggg caagtcaggc tgaacatcgc cccgcaaccg gtcttgccca cgcctgacca
      781 ggtacgcctt gatggctcga tccaccacgg cggcaggttc caacggctcc gggttcagca
      841 cggtcctgcc ggcatccaac cgagatacat ccaggaaatc gttgaccagc gcctccatcc
      901 gaagcccctc cttgtagatc agctccaggg cgctacgccg ttcttctttc gtcgcctctc
      961 gattcagcag gaactctgag aacccgatta tgctggtgag cggggtccga aactcgtggg
     1021 agacgaccga gatgatgttg gtgcgcaacc ggtccatctg ccattcgcgg ctcacatccc
     1081 tccaaaccga aaccgccccc acgacttgca tcaactcgtc tcgaatcggc gcagcactca
     1141 ccagcaccgg tagaagctcg ccccacgcag tctggacgaa gacggtgatg gggtctgtgc
     1201 ccgttccctc ctgcaaagcg gtacgcagcg ggcaagaggt ggaacagagg cgattgccct
     1261 cgtcatctgt gtggcacatc acctccccgc acatgctgcc caccaattga tccgcacggt
     1321 aaccgaccat ggcctccagc caagggttga catcggtgat cctcatgttc acgtcgacgg
     1381 cacacacacc ctcgctcatg gaggccagca acgcccccgt ccgggacttc tgggcctgaa
     1441 cctcttgaaa aagggtggcg ttctgtagag ccacggccac cgtcccggca aaagcgccgg
     1501 ccagggccac atcgtcgtcg gagaaaccgg ggcactccgg ccgacggccg aaaaagatga
     1561 ccccgatggc acggtccccc acggccattg gggcaactac tgccgtcgac attcccatcc
     1621 gcaccagcat ccgcgactcc aatggggaaa gcgatggatc gcccggcgtc atcaacacgg
     1681 ggtgccggct cgccacggct cggtaggatc ccggatactg ggtggccgtc atctcgatgc
     1741 cctcacccat tgccgactcg ccgtaacaat cgcaggctgc cctcgacacc aggacccccc
     1801 tatccgaatc catggtggct accgcgcaac ggtc
//
LOCUS       DATALS010000156         4094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_322346, whole genome shotgun sequence.
ACCESSION   DATALS010000156 DATALS010000000
VERSION     DATALS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322346"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VHS06_03400"
     CDS             <1..338
                     /locus_tag="VHS06_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987198.1"
                     /translation="HAASSVEWSPLFTYHVERNRLIMLAKNAPLRLALREHLRYATSL
                     MLNLGRFGRAVLLRSPDLAVLTRRIQIQLRVAGWLISHLFGTIAKRRHLAAQERVPAS
                     KLMDWMVTG"
     gene            335..1918
                     /locus_tag="VHS06_03405"
     CDS             335..1918
                     /locus_tag="VHS06_03405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2987199.1"
                     /translation="MKVGIYNRWLHTMGGGERYTVIAAQALAAENQVELITHRPTSLQ
                     ALESKLHVDLKGITVRCIPDLPFHRLGDYTEEYDLFINASFMSFVPSRAKKSVLFVFF
                     PFPVDKTAFGLFKRKVGLSLRHELIVPEYGEGFYGLQQVGGGWYRWTAEKALVHLPVV
                     RQGAAMRVRMMVGSFRPGGTTPVPLKVSVQGRQLAEISLSPTDGNYLPLEATIPGEAT
                     IGEKVTLVLETETFNPQGGPGFEDDFRDLGVAVAKVKVMGMRNQIYDLLFERLVKEAG
                     LRLAGIPDNLSLRYVDSYDLITPISEYVQKWLNIYWGKKGEILYPPVDVDVYDPTRPK
                     RNVILNVGRFFSGSHNKKHMVMIDAFKRLCRRGLSGWELHLVGGAAPGKIHAEYLEKV
                     RKKAQGYPIYIHADAPFSELQDLYEEASIYWHASGFGENENRNPIRFEHFGITTVEAM
                     AAGAVPVVIAKAGQLEIVEQGKSGFLWNSMEELEKYTLQVATDSALRATLQQGAIARS
                     RAFDQDSFKRRLLELIARI"
     gene            1922..3151
                     /locus_tag="VHS06_03410"
     CDS             1922..3151
                     /locus_tag="VHS06_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2987200.1"
                     /translation="MFKMTPTEAREFLTGFDHCSYQEAVDYLNVALDRLLVTLNMMPD
                     LGEGAKVLELGSTPYFMTALMMEQFPYQLELANEHDRLKLDGGHVRLINRKLGIDRSF
                     DYKTFNIETDRFPYDDGSLDAVIYCEMIEHLMHDPTHSLYEIHRVLKPGGYLLLSTPN
                     PFRYTNYFRFMRAQNIYPPFSGWGPYARHNREFSAQELRLLLTQCNFELETLFTAYDP
                     AYDHPTRFNSGRIIKWLFDHGMFKENMDVIHLRARTHGKAIYRYPPELYLDVHVYQRI
                     VNDNIEMGVNDEAQLGPGYYKLENFPPYVRWTARTAEAKLLFGGQQTLGIRFYSGPKE
                     LGRNVDGAIEIGPESHGFDVAAGEWVELKFPTPVAEHGPLQVTIKINKPWVPCRTVQW
                     SADSRDLGVAVQRIWLE"
     gene            3158..>4094
                     /locus_tag="VHS06_03415"
     CDS             3158..>4094
                     /locus_tag="VHS06_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987201.1"
                     /translation="MAATDAMVRADKSEQQPDTPMRRDRTVGWLLFAFFLALYLVTGG
                     GHGYSPDGEFAWRMAGSLIMDPTHEYLKDSRSRSGLDQWGIMVPVLAQPLTIIGESLA
                     PHLPQKDYVILNGQTFILGMFKGKDSPSDAPTVGAANTGAATTYTQTGLKNGPATKLT
                     VISFLALSSEIPQGATVAELTLVDSDGNQLVFPIRAGVESAEWNRGKGATPSQHQMAN
                     VASIWGGNPNGRNYYAEFPLDGTRTIKEFSVHYLQPIGHLYIRSMGLTNQETGAFEKI
                     PDTARFWSERENDQLFGRMFFSAYNAIITAIGCVLL"
BASE COUNT          886 a         1251 c         1190 g          767 t
ORIGIN      
        1 tccatgcagc cagcagcgtc gagtggtcgc cgcttttcac ctatcatgtg gagcgcaacc
       61 gactgataat gctggcaaag aacgcgccgc taaggctggc gctgagggag catctccgct
      121 acgctacgtc gctgatgcta aacctcggtc gcttcggccg tgccgtgttg ctgcgcagtc
      181 ccgacctagc ggtgctgaca agacggatcc agatccaact tagggtggcg ggctggctga
      241 tatctcacct cttcgggact attgccaagc ggcgccacct ggcagctcaa gagagagttc
      301 cggcttcgaa gctgatggat tggatggtaa ccgggtgaag gtaggcatat ataatcgctg
      361 gctgcacacc atgggcggcg gcgaacgcta tacagtcata gccgcacagg ccctggcagc
      421 cgaaaaccag gtggaactga taacgcatcg ccccacctcc ctgcaggcac tggaatccaa
      481 gctgcacgtg gacctgaaag ggatcacggt gcgctgcatc cccgacctcc ccttccaccg
      541 ccttggggat tacacggagg agtacgacct cttcataaac gcctcgttca tgagctttgt
      601 gccatcacga gcgaagaaga gtgtcctctt cgtcttcttc cctttcccgg tggataagac
      661 agccttcggc ctcttcaaac gcaaggttgg gctgtcgctc cgccacgaac tgatcgttcc
      721 cgagtatggc gaggggttct acgggctgca gcaggtcggc ggtggttggt accgttggac
      781 cgcagagaag gcgctggttc acctgccggt ggttcgccag ggcgccgcga tgcgggtgcg
      841 aatgatggtt ggcagcttca gacccggtgg gacgaccccc gtgccgctca aggtctcggt
      901 ccagggcagg caactggccg aaatctccct ttctcccacc gacggcaact acctcccatt
      961 agaggcaacg atcccaggcg aggcgaccat cggagagaag gtaacgctgg ttctggaaac
     1021 cgagaccttc aacccccagg gaggccccgg cttcgaggac gacttcagag atttgggagt
     1081 ggctgttgcc aaggtcaagg tgatgggaat gcggaaccag atctacgacc tgctcttcga
     1141 acggctggtc aaagaggccg ggcttcggct cgccggcatt ccggacaatc tgtcgttacg
     1201 ttatgtcgac agctatgatc tgatcacccc aatctccgag tacgttcaga agtggctgaa
     1261 catctactgg ggcaagaagg gagagatcct ctacccgccc gtagatgttg acgtgtacga
     1321 cccgactcgc cccaagcgca acgtgatcct gaacgtcggc cgcttcttca gcgggtccca
     1381 caacaagaag cacatggtga tgatcgacgc cttcaagcgg ctgtgccgaa ggggcctttc
     1441 gggctgggaa cttcacctgg taggtggagc ggcccccggc aagatccacg ccgagtatct
     1501 ggagaaggtg aggaagaagg cccagggcta cccaatctac attcacgccg atgccccctt
     1561 ctcggagctt caggacctgt acgaggaggc atccatctac tggcacgcca gcgggttcgg
     1621 ggagaacgag aatcgcaacc cgatccgctt cgagcacttt gggataacga cggtggaagc
     1681 tatggccgca ggcgccgttc cggtggtcat cgccaaagcc gggcagttgg agatcgtcga
     1741 gcagggtaag agcggattcc tgtggaacag catggaggag ttggagaagt acacactgca
     1801 ggtcgccacc gactcggctc tcagggcaac actacagcag ggcgccatag cccgatctcg
     1861 cgccttcgac caggacagct tcaagcgaag gctgctagaa ctgattgcga gaatttaagc
     1921 tatgtttaag atgaccccca ccgaggccag ggagttcctg accggctttg accattgctc
     1981 ctatcaggag gcagtggact atctgaacgt cgcgctggac cgattgctgg tcacgttgaa
     2041 catgatgccc gacctggggg agggggcgaa ggtactggag ctaggatcca ccccctactt
     2101 catgacagca ctgatgatgg agcagttccc atatcagctc gagctggcca acgagcacga
     2161 tcggctgaag cttgacggcg gccacgtgcg gttgatcaac agaaagctgg gcatcgaccg
     2221 ctccttcgac tacaagacct tcaacatcga gaccgaccga ttcccctacg acgacgggag
     2281 tctggatgcc gtcatctact gcgagatgat cgagcacctg atgcacgatc cgactcactc
     2341 cctgtacgaa atccatcgtg tgctgaagcc cggcgggtac ctgctgctca gcacgcccaa
     2401 ccctttccgg tacacaaact acttccggtt catgcgggca cagaacatct accctccctt
     2461 ctccggctgg ggaccgtatg ctcgccacaa ccgagagttc agcgcccagg aactgagact
     2521 gctgctgacc cagtgcaatt tcgaactgga gacactgttc actgcctatg accctgccta
     2581 cgaccaccca acccgattca actccggcag aatcatcaag tggctattcg atcacggcat
     2641 gttcaaggag aacatggatg tgatccacct gcgtgctcgg acgcacggca aagcgatcta
     2701 ccgttaccca cctgagttgt acctggacgt tcacgtctat caacggatcg tcaacgacaa
     2761 catcgagatg ggcgtcaacg atgaggcgca gttgggtccc gggtactata agttggagaa
     2821 ctttcctccc tacgtacgat ggacggcccg gactgccgag gccaagctgt tgttcggagg
     2881 ccagcaaacc ctgggcatcc gcttctactc cggtccaaag gaattgggcc gcaatgtgga
     2941 cggggctatc gagattggcc ccgagtccca cggcttcgac gtagcagccg gcgagtgggt
     3001 tgagctcaag ttccctacac cggtcgccga gcacggcccc ctgcaggtga ccatcaagat
     3061 caacaaaccg tgggtcccct gcagaacggt acagtggagc gccgatagtc gcgacttggg
     3121 agtcgcggtc caacgcatct ggctggagta accgcacttg gccgccaccg acgccatggt
     3181 ccgagcagac aagtctgaac agcaacccga tacccccatg cgtcgcgatc gcacggtcgg
     3241 ctggttgctg tttgccttct tcctggccct ctaccttgtt acgggcggcg gccacggcta
     3301 cagtcccgac ggagagttcg cctggcgaat ggccggcagc ctcatcatgg atccaaccca
     3361 cgagtacctg aaggactccc gcagccggtc cgggctggac cagtggggca tcatggtccc
     3421 ggtgctggcc caacctctca cgataatcgg tgagtcgctg gcgccgcacc tgccgcagaa
     3481 agactacgtc atcctgaacg ggcagacatt catcctgggc atgttcaagg gtaaggactc
     3541 cccgtcggat gcacctaccg tgggtgcggc caataccggc gcggcgacga cctacaccca
     3601 aacaggactg aagaatggtc cggccacgaa gctgacagtc atatcgttcc tggccctatc
     3661 tagcgaaatc ccccaaggag ccacggtcgc agaacttacc ctggtggatt ccgacggcaa
     3721 tcaactcgtc ttccccatca gggcgggcgt tgaaagcgcg gagtggaacc gcggaaaggg
     3781 cgccacccct tcacaacacc aaatggctaa cgtggcatcg atctggggcg gcaaccccaa
     3841 cggccgcaac tactacgcgg agttcccact ggacggtacg cggacgatca aggagttcag
     3901 cgtccactac cttcagccga tcgggcactt gtacatccga tcgatggggc tgaccaacca
     3961 agagacggga gccttcgaga agatccccga taccgcccga ttctggtcgg agcgggagaa
     4021 cgaccaactg ttcggacgca tgttcttctc ggcctacaac gccataatca ccgccatcgg
     4081 ctgcgtcctg ctct
//
LOCUS       DATALS010000157         4706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_240277, whole genome shotgun sequence.
ACCESSION   DATALS010000157 DATALS010000000
VERSION     DATALS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240277"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VHS06_03420"
     CDS             complement(<1..253)
                     /locus_tag="VHS06_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987202.1"
                     /translation="MFRHTKTLSTRRQWWRVATLITASAMMLGLTVACSGGTAPQPTA
                     APAKATSAPAASSAAPTNAAAPAKVQHVKLSDQAAEDHPE"
     gene            complement(390..1670)
                     /locus_tag="VHS06_03425"
     CDS             complement(390..1670)
                     /locus_tag="VHS06_03425"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX2987203.1"
                     /translation="MATVLFATFVVLLVFAVPVSYALGLSSVAALVFKGGIPLTIVAQ
                     KMFTGLDSFPLLAIPFFILAGSLMENGISSRLIRLATVFVGHIKGGLGMVVVLSTMLF
                     SGLSGSSAADTAAIGSIMIPEMSKKGYPRGLATAVVASSGGFAILIPPAITMIVYAVV
                     ANVSVGYLFFAGFLPAGLMAVAMMTAMYFVADRLDIPQQPKATCGEMVRATLSALPPL
                     GMPVIVLGGIRVGAFTPTEGAIVAVVYALILSVFIYRELNIRQVPKILVASAELTGIV
                     TLLIGMSSTFAWVVASQQIPQAVARGMLSISDNPIVFMLMVNFLFLFVGCIMDATPAV
                     IILVPILYPLATQLGIDPIHFGTVLVANLGIGLVTPPVGVVLYVAASIGKVKIEEVFK
                     YLLPFFAALIISLLIISYLPDVTLFVPRLFGYEH"
     gene            complement(1682..2119)
                     /locus_tag="VHS06_03430"
     CDS             complement(1682..2119)
                     /locus_tag="VHS06_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX2987204.1"
                     /translation="MASAVVFAQVLFRYVFEQPLAWAEEGSRFLFIWVALLGAALAVR
                     NRSHFAIGIIVDRCPKPIQEAVRVLTALLGTYIFGLMVTEGWTLVLLNQNQESPAIGL
                     TMSIPYAVIPISGLLMIYFTWSDLILHWRHENDVDDADALLVD"
     gene            complement(2260..3351)
                     /locus_tag="VHS06_03435"
     CDS             complement(2260..3351)
                     /locus_tag="VHS06_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX2987205.1"
                     /translation="MYRVSSWRRSFATLSIAVMAVGLLIVATACGGGAPAQPTSAPSK
                     AAPAATAGSAQQPAAAAGAQTVKFASVAAEDHPHHKAFLFFADRVKELTNGKLNVQVF
                     ANSQLGNEREYVEQLQIGQIEFAKTSSAVIGPFIPQFQVFDLPYVFKSREHLFKQTDG
                     EVGQTLIKLAEQKLNVKVFGFFDAGTRSIFNSKKPINTPADMKGMKLRVMENPLMIQT
                     FNSLGALATPMAVGEQYSGMQQGVIDGAENSPVFYRSQKFYEVAKYYSYTDHFMTPDV
                     PLMSLKWYNAQPKEIQQAVDQAGKEMVQKEREIWLEYEKFVADDLTKNGVQFNNVQDK
                     NAFAKLVEPLYSKFGEKIGQDLVNKAAAQ"
     gene            complement(3943..>4706)
                     /locus_tag="VHS06_03440"
     CDS             complement(3943..>4706)
                     /locus_tag="VHS06_03440"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX2987206.1"
                     /translation="GGECLKMRFDGKVVLVTGGARGIGGGISRLFGRLGAKVAVNYVS
                     NDEAAESLAAEMRGEGIDVALFKADVSDYSAAERLVQGVVERFGALDVLVNNAGINIP
                     PHSIFDMPPETWQRVISTNLSSMYNVTRPAALAMKDGSGGAIVNIASNVISTGGGSGP
                     AYAASKGGVQGFTRSLGKDLAPHGIRVNAIAPGVTETELVASLPPDVQARLRAPAPAG
                     RIGQPKDVAGLAAFLASDYASFIVGQTIFVDGGKY"
BASE COUNT          996 a         1397 c         1419 g          894 t
ORIGIN      
        1 gctccggatg atcctcggcc gcctggtcgc tcagcttaac gtgctggact tttgcaggag
       61 cggcggcatt ggtcggtgcc gcggacgaag ctgcaggtgc cgatgtggcc ttggcgggtg
      121 ctgcagtggg ctgaggggca gtccccccgc tgcaggccac ggtgagcccc agcatcatcg
      181 cggacgcggt gatgagggtt gccactcgcc accactggcg acgggtggat agggttttcg
      241 tgtgcctgaa cactgtctcc tcctctttgt cctatcgctt ccggagccgt cctccggaat
      301 actacggctg aatgtcacca gagcgtgttc ccgtttcggg gcagtcgcgc ggcggcgggc
      361 ccagaattgc gtgcttgtcg atgatgtccc tagtgctcgt agccgaacag cctggggaca
      421 aatagggtga cgtctggcag gtaactgatg atcagcagtg aaatgatcag tgccgcgaag
      481 aacggcagca ggtacttgaa gacttcctcg atctttacct ttccgatgct ggctgctaca
      541 tacagcacga caccgacagg tggggtgacg agcccgatgc ccaggttggc caccagcacc
      601 gtcccgaagt ggatcgggtc tatgccgagc tgcgtggcta gagggtatag gatcggcaca
      661 aggatgatga cggccggggt tgcgtccatg atgcagccga cgaacaggaa caggaagttc
      721 accatgagca tgaatacgat ggggttgtcc gagatcgaca gcattccccg ggctaccgcc
      781 tgtggtattt gctgggaggc gaccacccaa gcgaaggttg acgacatgcc tatcaacagc
      841 gtcacgatgc cggtgagttc cgccgaggcg acgagtatct ttggtacttg tcgaatgtta
      901 agctctctat atatgaacac cgagaggatc agggcgtaga cgacggcgac gatcgcaccc
      961 tccgtggggg tgaaagcgcc gacccgaatt ccgccgagga cgatcaccgg catcccgaga
     1021 ggcggaagtg ccgagagagt ggcgcgcacc atttctccac aggtcgcctt tggctgctgc
     1081 gggatatcga ggcggtcggc cacgaagtac atcgctgtca tcatcgcgac cgccatcagg
     1141 cccgcgggca ggaaccccgc gaagaagagg tagccgacgg aaacgttggc caccacggcg
     1201 tagacaatca tggtgatggc gggcgggatc aaaatggcga agccaccgga ggaggcaacc
     1261 accgccgttg ccagtccacg tggatagccc ttcttagaca tctcagggat catgatggat
     1321 ccgatggcag cggtatcggc ggccgaggat ccggagagtc ccgagaacag catcgtcgag
     1381 aggacaacca ccatcccgag gccacccttg atgtggccga cgaacactgt ggcaagccga
     1441 atcaggcggg aggagatacc gttctccatg aggctcccag cgaggatgaa gaagggtatg
     1501 gccaggagcg ggaaggagtc cagcccggtg aacatcttct gcgccacgat ggtgagaggg
     1561 atgccaccct tgaagacgag ggctgctacg gacgaaagtc ccagcgcgta tgaaactgga
     1621 actgcaaaga ccaatagaac gacgaaagtc gcgaagagta cggtagccat agcagtcccc
     1681 cctaatccac tagcagcgca tcggcgtcat cgacgtcgtt ctcgtgccgc cagtgcagta
     1741 tcagatcaga ccaggtgaag tagatcatca acagcccact gatggggata accgcatatg
     1801 ggatactcat cgtgagcccg atggcgggcg actcctggtt ctgattcagg aggaccagcg
     1861 tccatccctc ggtcaccatc agtccgaaga tgtaggttcc caggagggcg gtcagcaccc
     1921 gcaccgcctc ttggatgggc tttgggcagc ggtcgactat tataccgatg gcgaagtgcg
     1981 atcggttcct aaccgcgagc gccgcgccca gcagggctac ccagatgaat aggaaacgtg
     2041 agccctcctc tgcccaggcg aggggctgtt cgaagacgta ccgaaaaaga acctgcgcga
     2101 agacgacggc tgatgccacc gccagtatcg cgcctgctac gaacccgacg agggaataca
     2161 tgagaccgtt aaaccgcttg atggtacacc tccgtgtatc gaaaggcgct gttcccagag
     2221 gagtggatca tccgctcctc tggaaacagc tggattgtcc tactgcgcgg ccgccttgtt
     2281 aactagatcc tgtccgatct tctctccgaa cttagagtac aggggctcga ccaatttggc
     2341 gaacgcgttc ttgtcctgca cgttgttgaa ttggacgccg ttcttcgtga ggtcgtcggc
     2401 aacgaacttc tcgtactcca gccagatctc gcgctctttc tggaccatct ccttgccagc
     2461 ctggtcgacg gcctgctgga tctccttggg ctgtgcgttg taccacttga gactcattag
     2521 cggcacgtcg ggtgtcatga agtgatccgt gtaggagtag tacttggcca cttcgtagaa
     2581 cttctgcgag cgatagaaca caggggagtt ctcggccccg tcgataacgc cctgctgcat
     2641 gccggagtac tgctcgccaa cggccattgg agtggccaag gcgcccaatg agttgaatgt
     2701 ctggatcatc agggggtttt ccatgacccg gagcttcata cccttcatat cagccggcgt
     2761 attgatgggt ttcttggagt tgaaaatgct gcgagtaccg gcgtcgaaga agccaaagac
     2821 cttgacgttc agcttctgtt ccgcgagctt aatcagcgtc tgtccgactt cgccgtccgt
     2881 ttgcttgaag agatgctcgc ggctcttgaa cacgtatgga aggtcgaaca cctggaactg
     2941 gggaatgaat gggccgatga cggccgaaga agtcttggca aactcgatct ggccgatctg
     3001 cagctgctca acgtactctc gctcgttgcc tagctgcgag ttggcaaaga cctgcacgtt
     3061 cagcttgccg ttggtcaact ccttcacccg atcggcaaag aagaggaacg ccttgtggtg
     3121 aggatgatcc tcggctgcca cggaggcaaa cttcactgtc tgggcaccgg cagcagcagc
     3181 cggttgttga gccgagccag cggtcgcggc cggtgccgcc ttactcgggg cggaagtggg
     3241 ctgagccggg gccccgcccc cgcaagcagt agccacgatt agaagcccca ccgccatcac
     3301 ggcaatactc aacgtcgcga aggaacgtcg ccaggaacta acccgataca taatccctcc
     3361 cgtattcact gacctatctc gtgccgatct gcacgatgag caagtggtta gacgaagaag
     3421 tacacctcct tttcgcaggc tatcaacatg gagaatcccg ccggcatggt caacacgacc
     3481 ctccgccacc cgcgcgaggc cggccaacag aataccaaca cgattagaaa tggcggaagg
     3541 ggcgctgcgc catcctagcg ttgggatgcc gcacagcgtc ggtactcgac gaaacccggt
     3601 gaaccggcgg gctaccgtgg aaggtggact gcccgccgcg gaaggtgccc aagggcaaac
     3661 ggtttccacg gcgacgatat gataattcgg tggtcctacc ttgtcaatga ctgcgagtcc
     3721 atcagatgac cacctgctgg gcttctctgg gggcaagttc aaggggttgg acttcagcag
     3781 aagcgggcct tgccaaaaga acgacaaccg gatcatggag cagaagagcc acgctggtcg
     3841 atgggggtgg acggaggaga aagcgccgat cggaggctca tatgcgagga aggggtgact
     3901 ctgcactgca aagccacccc ttcctcgcat tagtgtcctc agtcagtact tgccgccatc
     3961 gacgaagatc gtctggccca ctatgaagga agcataatcc gaggccagga aggcggccag
     4021 cccagccaca tccttcggct gcccgatcct tcccgcaggg gccggggccc ggagcctggc
     4081 ctgcacgtcc ggtggtagag aagccaccaa ctcggtctcc gtcacccccg gcgctatggc
     4141 gttgacccta attccgtggg gcgccagatc ctttcccaac gagcgcgtga acccctggac
     4201 cccgcccttg ctggccgcgt atgcggggcc ggaccccccg cccgtggaga tgacgttgga
     4261 ggcaatgttg acgatagcgc cgccgctgcc atccttcatc gccagcgccg ccggcctggt
     4321 gacgttgtac atggagctaa gatttgtgct gatcacgcgc tgccacgtct ccggtggcat
     4381 gtcgaatatg ctgtggggag ggatgtttat ccctgcattg ttgaccagga catccagagc
     4441 cccgaagcgc tcgaccacgc cctgaaccag tctctcagcg gcgctatagt cggacacgtc
     4501 cgccttgaag agggcaacgt cgataccctc gccccgcatc tccgcagcca gactctccgc
     4561 ggcttcatcg ttactcacgt agttcacggc cacttttgcg cccagcctac caaacagccg
     4621 ggagatgccc ccgcctatcc cacgagcgcc ccctgtgacc agaaccacct tcccgtcgaa
     4681 acgcatcttt aaacactccc ctccac
//
LOCUS       DATALS010000158         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_15867,
            whole genome shotgun sequence.
ACCESSION   DATALS010000158 DATALS010000000
VERSION     DATALS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15867"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1276)
                     /locus_tag="VHS06_03445"
     CDS             complement(104..1276)
                     /locus_tag="VHS06_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2987207.1"
                     /translation="MTDWREVESKVYMGVFRRTPLVIVRGEGNRVWDDKGKCYLDFVA
                     GWAVTSLGHCHPAIVEAVRKQVGTLIQTSNQYYTVPQLELGQLLVDNSALDRVFFANS
                     GAEANEGAVKLARKWGKLKLNGAYEVISTLKSFHGRTLSMVAATGKPAYQKDFTPLPA
                     GFVNIPYNDMDALENAATEKTCAILLEPIQGEGGVNIPDEEYLSNVRAFCTDHNILMM
                     LDEVQTGVGRTGTLWAYEQFGIEPDVMSVAKGLAGGVPIGAFLSKEDVSVLAPGDHGS
                     TFGGNPLACAAGVAVMRYILENDVPGHVRKVGAHLMDRLLGLKARHPEIADVRGRGLL
                     AAIEFSEQKTADILARSLERGLLLNAVSPTIIRLFPALTITEGEIDEAMGILESAL"
     gene            complement(1295..1432)
                     /locus_tag="VHS06_03450"
     CDS             complement(1295..1432)
                     /locus_tag="VHS06_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987208.1"
                     /translation="MAVSPIVMNALKRAALAGFIAALTVFMDEVRRSEEKEREREKKA
                     K"
     gene            complement(1454..2284)
                     /gene="argB"
                     /locus_tag="VHS06_03455"
     CDS             complement(1454..2284)
                     /gene="argB"
                     /locus_tag="VHS06_03455"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX2987209.1"
                     /translation="MPYTAALSRLIEAIPYINRYIGKTVVVKMGGSTMDSDQTVLQDV
                     ATLKRLGINPILVHGGGPEISEWMRRVDKKPQSVDGLRVTDQETLDIATMVLRGKVNA
                     ELVAMLNGMGAPAVGLCGADAGLFQAVQRDVRLGYVGDISRVDLRILSVLTGQGYLPV
                     VAPIGLGEGGRLLNINGDTAAGELAAAISAEKLIFFTDVAGIQDSAGNLLVQLTTAEA
                     QGLISSGTVTGGMIPKVKACVKALSTVGRTHIIDGRVPHALLRELFTDSGVGTMIVAS
                     "
BASE COUNT          429 a          791 c          628 g          455 t
ORIGIN      
        1 gggcgacccg ccctaccgga cacacgtagg gcgggtcgcc cccgacccgc ccgtcttgcc
       61 gtgtacgcgg ggtgccttaa ccccgtcgcc tcgttgaccc cgcttacagg gcgctctcca
      121 ggatgcccat tgcctcgtcg atctccccct cggttatggt gagggccggg aacagtctga
      181 tgatggtcgg agacactgcg ttaagcagta gaccgcgctc caggctgcgc gccaaaatgt
      241 cggccgtctt ctgctcgctg aattcgatag ctgccaggag acctcggccg cgaacatccg
      301 cgatctcggg atggcgggcc ttcaacccca gcaaccgatc catcaggtgt gctccaacct
      361 tcctgacgtg gccagggacg tcgttctcga gaatgtagcg cattacagcc acgcccgcag
      421 cgcaagcgag cggattgccg ccaaaggtag agccgtggtc gccgggggcc aatacggaca
      481 cgtcctcctt gctcaggaac gcacctatcg gcacgccgcc cgccaacccc ttggccaccg
      541 acatcacatc cggctcgatg ccgaactgct cgtaggccca gagggtgccg gtccgcccaa
      601 cgccggtttg cacctcgtcg agcatcatca ggatgttgtg atcggtgcag aaagccctga
      661 cgttggagag gtactcctcg tcgggaatgt tcaccccgcc ctccccctgg atgggctcca
      721 ggagaatggc gcaggtcttc tccgtggctg cgttctctaa cgcatccatg tcgttgtacg
      781 ggatgttaac gaagccggcc ggcagcgggg tgaagtcctt ctggtaagca ggcttgccgg
      841 tggccgcgac catcgacaag gtgcgaccat ggaaggactt gagagtgctg atcacttcgt
      901 aggcgccgtt gagcttcagc ttgccccact tccgcgccaa cttcacggcg ccctcgttgg
      961 cctcggcgcc gctgttggcg aagaagactc gatccagcgc ggagttgtcc accagcagct
     1021 gccccagctc tagctgaggc acggtgtagt actgattcga tgtctggatg agggttccga
     1081 cttgctttcg cactgcctcg acgatggctg gatggcagtg gccaaggctg gtgacggccc
     1141 acccggctac aaagtccaga tagcacttgc ccttgtcatc ccagactcgg ttcccctcac
     1201 cacggactat taccagaggg gtgcggcgaa acacccccat gtatacttta ctctcaactt
     1261 cgcgccagtc cgtcatcgca ctctactcct ccctctactt ggccttcttc tctctctccc
     1321 gctctttctc ttccgatcgt cggacctcat ccatgaagac ggtcagggca gcgatgaatc
     1381 cggcgagggc ggccctcttg agagcattca tcacgatagg agacactgcc acgctacacc
     1441 tcccgctctg taatcacgac gccacgatca tggtgcccac gcccgagtcg gtgaacagtt
     1501 cccgcaacag cgcgtgaggc actcgaccat cgatgatgtg cgttctccca acggtgctca
     1561 gcgccttaac gcatgccttc accttaggga tcataccacc ggttacggtc ccagagctta
     1621 tcagtccctg cgcctcagcg gtagtcaact ggaccagcaa atttcccgca gagtcctgga
     1681 tccctgccac gtccgtaaag aagatcagct tctcggcgga gatagcagca gccagttcgc
     1741 cggcggcagt atccccgttg atgttcagca gccggccgcc ctcgcccagc cctatgggag
     1801 caaccaccgg caggtacccc tggccggtca gcacggacaa aatccgtaaa tctactcgac
     1861 taatatcccc aacgtaaccg aggcggacgt ccctctgcac agcctggaaa agtcctgcat
     1921 ctgcgccaca cagccccacc gcgggtgcgc ccatcccgtt cagcatcgcc accaactccg
     1981 cgttcacctt acccctcagc accatcgtgg cgatgtccag cgtttcctgg tccgtgaccc
     2041 tgagcccatc cacggactgc ggcttcttat ctacccgccg catccactcc gagatttcgg
     2101 ggccgccgcc atgcaccagg ataggattga tccccagccg cttcagggta gccacgtcct
     2161 ggagaacggt ctggtccgaa tccatggtgc tcccccccat cttcaccacg actgtcttcc
     2221 cgatgtagcg attgatgtag gggatggcct ctatcagtct gctcaaggcc gcagtgtacg
     2281 gcaacgaccc gaactggggg cga
//
LOCUS       DATALS010000159         2969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_97573,
            whole genome shotgun sequence.
ACCESSION   DATALS010000159 DATALS010000000
VERSION     DATALS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97573"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..467
                     /locus_tag="VHS06_03460"
     CDS             192..467
                     /locus_tag="VHS06_03460"
                     /inference="COORDINATES: protein motif:HMM:NF041918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX2987210.1"
                     /translation="MMIIRYFADIRDLTGENERRWTGTASTLAELLSDLGGYYGPRFR
                     GRILTDSGLSETIIVLINGRDIRHLEGLNTPLGPDDTVSIFPVVAGG"
     gene            655..>2969
                     /locus_tag="VHS06_03465"
     CDS             655..>2969
                     /locus_tag="VHS06_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX2987211.1"
                     /translation="MAFLCSLVSLAGVLLNYPTILQGNVIDFRLLSWDGPIALAYHVD
                     GLSQLFALMGAGIGAAVLLYSIDYMAHDPGATRFYILIQVFIAGLVHLVYTSDLFLLY
                     LSWEAIGLCSFLLVGFWHRLPEAAYGARKVLVMTHLAGYGLLAAIIILFSRTGSTLWT
                     DPKVAAAFNTGIFLLILASAVAKSVQFPLHSWIPDAMAAPTPVSALLHAACYVKAGVY
                     LVARLHSLGPWPTEWQLTVIWLGTVTMLVGVLYAMIQSDLKRLLAYHTVSQIGYMMLG
                     LGLGTPLGIAAGLLHCLNHGLFKGGLFLCAGAVQHATGTRDMSVLGGLGKRMPKTTAL
                     WLVVAAGIAGVPLLNGFVSKWLVYSAALEAGQTIPAAVAWIVSIFTVFSFLKATSGVF
                     LGDETAEGSKAHESPRSMLAGTGVLAAGTLLLGVAPQLAVSYLIDPLLPALGMKSTIE
                     VSWLGLATTEGSWFATGGLLLAVVALGIGLLVYRMGKPAPAPATVALAATAAGSMQLT
                     IPMGEARTCQSGWQRAEGNTQHPALSSAPFTGGEPLVGSSRLLAVDFSLMVKHGLAPF
                     YQWVNVDMYYLGIWRWLLSLAGKIGRASLWLEERALATLLILAVATFALAALLVPGLV
                     SSTHAPRLAAHAAQAGGGTGALMVPIGIALAGLLLVAHASARDSLRLPLLAVAGILAL
                     VGLGTSGEMIRLLLLESAALVALLLVWREGKNRSAAHAYLLAILLSAAATVGGTTLLE
                     RGSATLALALLLAGLTLKFALFPLYLWLPKMA"
BASE COUNT          473 a          996 c          914 g          586 t
ORIGIN      
        1 cgctagcata agtacgtaga gagagaagcg cgtccagggc acgcaacgcg agcgagcgca
       61 gcgttgcgac ctggcgggtg cgaaggcgag cgagcacagc cttcgcgggt gcccccaaat
      121 tcactgtttc tttctaccta gatacctgtt ctggagtgcg gtgcgcgcac cgcactccag
      181 aacccacatg aatgatgata attcgctact ttgctgacat ccgagatctt actggggaaa
      241 acgagcgacg ctggacgggc acggcttcca ctctggcgga attgctgagc gacctcggcg
      301 gctactacgg tccccgcttc cgcggccgca tcctcaccga tagcggtctc agtgaaacga
      361 tcatcgtcct gatcaacgga cgagacatcc ggcatctgga ggggctaaac accccgctcg
      421 ggccggatga cacggtgtcc atattccccg ttgtagccgg gggatagccg gccggcgggt
      481 tgagacaacc cgcctaagac aaggtattgg ccgaggcgtt agagcccaac gtccggtacg
      541 gaggtcgcag tgtcgccagc atccttggtc cttcttccgg tagctgccct gctagtcggg
      601 gtgctgctcc aactattact tgctcgatcg ctatctgcac gaggcaaagg cttgatggcc
      661 ttcctgtgca gtctagtgtc cctggcaggt gtgctgctga attaccccac aatcctccag
      721 ggcaatgtca tcgacttcag gctcctctcg tgggatgggc ccatcgccct cgcctatcac
      781 gtggatggac tcagccagct cttcgccctg atgggcgcgg gcatcggcgc ggcagtcctc
      841 ctctactcca tcgactacat ggcccacgac ccgggcgcga ctcggttcta catccttatc
      901 caggtattca tcgctggact cgtccatctc gtctacactt cggatctctt ccttctctac
      961 ctgagctggg aggccatcgg cctctgctcc ttcctgctgg tcggcttctg gcacagacta
     1021 ccggaggcag cgtacggcgc tcgtaaggtg ctggtgatga cccacctggc aggctacggc
     1081 ctgctggcgg cgataatcat cctcttctcc cggacgggat ccaccctctg gaccgacccg
     1141 aaggtcgccg cggccttcaa caccggcatc ttcctgctga tactggcttc cgccgtcgcc
     1201 aagtcggtcc agttcccgct gcacagctgg atcccggatg ccatggcagc ccccacgccg
     1261 gtgagcgccc tcctgcacgc cgcatgttac gtcaaggccg gcgtctacct cgtcgctcgc
     1321 ctccacagcc tcggcccctg gccgacggag tggcagctaa ccgtcatctg gctcgggacg
     1381 gtgacgatgc tcgtgggcgt cctgtacgcg atgatccagt ccgacctgaa gcgactgctt
     1441 gcctaccaca cggtcagcca gatcggctac atgatgctgg gactcggact cggcaccccc
     1501 ctggggatcg ccgcggggtt gctccactgt ctgaaccacg ggctgttcaa gggtgggctg
     1561 ttcctctgcg ccggtgccgt ccagcacgcc actgggactc gagacatgag cgttctgggc
     1621 ggactgggca agcgaatgcc gaagaccacc gccctgtggc tggtggtggc cgccggcatc
     1681 gccggcgtcc cgctgctgaa cggcttcgtc agcaagtggc tggtgtacag cgctgctctg
     1741 gaggccggcc aaaccatccc cgccgcggtg gcgtggatag tcagcatctt cactgtgttc
     1801 tccttcctga aggccaccag cggagtattc ctgggagacg aaaccgccga aggttcgaag
     1861 gcccacgagt caccccgctc catgcttgcc ggaaccggcg tgctggccgc cggcaccctg
     1921 ctgctgggag tcgcgccgca gttggccgtc agctacctga tcgacccgct gctgccggcc
     1981 ctcgggatga aatcgactat agaggtttcg tggctggggc tggccaccac ggagggcagc
     2041 tggttcgcca ccggcgggct gctgctggcg gtagtggcgc tgggcatcgg cctactcgtc
     2101 taccggatgg gcaaacctgc gcccgcgcct gccaccgtcg cccttgccgc aaccgctgcc
     2161 ggctctatgc aactgacgat acctatggga gaggcgcgga catgccagag tggatggcaa
     2221 cgggctgagg gcaacactca gcacccagca ctcagctctg ctccgttcac cggcggcgag
     2281 ccgctggtcg ggtccagtcg gctactggcg gtggacttct ccctgatggt caaacacggc
     2341 ctggccccct tctatcagtg ggtcaacgtg gacatgtact acctggggat ctggcggtgg
     2401 ctgctcagcc tcgccggaaa gattgggcgt gccagtctct ggctggaaga gcgggcgctg
     2461 gcaaccctcc tgatcctggc cgttgccacc tttgccctgg cagcgctact ggttccaggg
     2521 ttggtctcct ctacccatgc tcctaggctc gcagctcacg cagctcaggc cggcggcggc
     2581 acgggcgccc tcatggtccc gataggcatc gcgctggccg gcctgctgct ggtggctcac
     2641 gcctcggctc gagactccct gagactgccc ctcctggccg tggcaggtat cctggccctg
     2701 gtcgggctgg gaacgagcgg cgagatgatc cggctgctgc tgctggagtc ggccgcattg
     2761 gtagcgctgc tcctggtctg gcgagagggt aagaaccgct ccgccgcaca tgcctacctg
     2821 ctggccatcc tgctctctgc ggcggccacg gtcggcggaa ccacgctgct cgagcgagga
     2881 tcggccacat tggccctggc gctactgctg gccggtctaa ccctgaagtt cgcactcttc
     2941 ccgctctatc tctggctacc caagatggc
//
LOCUS       DATALS010000160         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_52093,
            whole genome shotgun sequence.
ACCESSION   DATALS010000160 DATALS010000000
VERSION     DATALS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52093"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1510
                     /gene="dnaA"
                     /locus_tag="VHS06_03470"
     CDS             128..1510
                     /gene="dnaA"
                     /locus_tag="VHS06_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX2987212.1"
                     /translation="MQPAQLWDAALGELQLQMTKANFDTWLKDTFLVAQEDSHIVIGV
                     GSPFAVETLEQRLSPMIRKVLSGIVGHEVGIEFVVQQKKPRGRGKVATEVPLEGASRS
                     RNGRSRGESAAPSYTAEASLNPRYIFDSFVVGKGNQLAHAACQAVADDPGRAYNPLFL
                     YGGVGLGKTHLMQAIGHEILRRGMRVLYVSSETFTNEMIDSIREHRTEDFRNKYRRVQ
                     LLLVDDIQFIAGKVGTQEEFFHTFNAIHEGGGQIVISCDRPPKAISTLEDRLRSRFEW
                     GLIADIQPPDLETRIAILRSKCQSQGINVPEEVLEAIARKVQSNIRELEGSLNRIVAQ
                     ATVNGEPITVQTASAALNEVMFNISRRFIKPETIMAAVARYYHVSEEDLRGKARDKRV
                     VLPRQVSMYLLREETELPLVEVGRLFGGRDHTTVLHSVEKVGSEVTNGGPVAKDLAAL
                     RESIYSSSNN"
BASE COUNT          313 a          521 c          479 g          269 t
ORIGIN      
        1 gagtccggca agactgcacg aatccttccg tttgggcaac gtggccccgt tgttaccccc
       61 atccggctct gctaccattt gcgggcctcg ttcttctaac tagagtacgc ccttccggag
      121 acagtttatg cagccagccc agctatggga tgccgctctc ggcgagctcc aactccaaat
      181 gaccaaggcc aacttcgaca cctggctcaa agacaccttc ctggtcgccc aggaggattc
      241 ccacatcgtc atcggtgtcg gaagcccctt cgccgtagag accctcgaac agcggctatc
      301 ccccatgatc cggaaggtcc tctccggcat cgtcggccac gaggtgggca tcgagttcgt
      361 ggtgcagcag aagaagcctc gcggaagggg gaaggtcgcg acggaggtgc ccctggaggg
      421 cgcgagtcgc agcagaaacg gccgttccag gggggaatcc gcggctccca gctacaccgc
      481 ggaggcatcc ctcaacccac gctacatatt cgacagcttc gtcgtcggca agggcaacca
      541 gctggctcac gccgcctgcc aggccgtggc cgacgacccc ggtcgagcct acaacccgct
      601 cttcctctat ggaggcgtcg ggctgggcaa gacccacctg atgcaggcca tcgggcacga
      661 gatcctgcgc cggggaatgc gggtgctcta cgtctcctcc gagaccttca ccaacgagat
      721 gatagactcc attcgggagc atcgaaccga ggatttccga aacaagtacc gccgggtgca
      781 gctgctgctg gtggacgaca tccagttcat cgccggcaaa gtcggtactc aggaggagtt
      841 cttccacacc ttcaacgcca ttcacgaggg aggcgggcag attgtcatct cctgcgaccg
      901 gccgcccaag gcgatctcga ccctggagga tcggctgcgc tcccgcttcg agtgggggct
      961 gatcgccgac atccagccgc cggatctgga gacccgcatc gccatcctcc gctccaagtg
     1021 ccagagccag gggatcaacg tcccggagga ggtgctggag gcgattgccc gcaaggtgca
     1081 gagcaacatc cgtgagttgg agggctccct caacaggata gtggcgcagg ccacggtcaa
     1141 cggcgagccc atcaccgttc agacggcctc ggccgcgctg aacgaggtga tgttcaacat
     1201 ctccaggcgg ttcatcaaac cggagaccat catggccgcc gttgcacgct actaccacgt
     1261 ctccgaggaa gacctacgtg gaaaggctcg ggacaagcgc gtcgtgctac cccgccaggt
     1321 gtcgatgtac ctgttgcgcg aggagaccga gctccccctg gtagaggtgg gcaggctgtt
     1381 cggcgggcgc gaccacacca cggtcctcca cagcgtggaa aaggtgggga gcgaggtgac
     1441 caacggcggc ccggttgcca aggatctggc agcgctcagg gagtccatct actcctcatc
     1501 gaacaactag cccctgccgc cccttctgta gtgcagcgcc aagccctcgc acaaagtcag
     1561 gcgtagctct cttcgggtga tc
//
LOCUS       DATALS010000161         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_196488, whole genome shotgun sequence.
ACCESSION   DATALS010000161 DATALS010000000
VERSION     DATALS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196488"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VHS06_03475"
     CDS             <1..300
                     /locus_tag="VHS06_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HEX2987213.1"
                     /translation="GNIESYEDFLDELAQVFAGCQRVLKPKRYLAVVVSDFRHGPQFV
                     LFHADLAKRIEQIGLPLRGITILLQDNKNLYPFGVPNAFVSNVHHQYILIHQKPA"
     gene            353..1522
                     /locus_tag="VHS06_03480"
     CDS             353..1522
                     /locus_tag="VHS06_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HEX2987214.1"
                     /translation="MGDVGAPSGPRLAATLTVGQASCLSRGLRQARRLSHRRLLSQRA
                     GGLRMLNLQPVPRHALFAMPGWHVWCGTICRCPVPLGLPSPGDRLRSHGRGAGGEGSR
                     YALLFSRWPTATTFNGWVTHSEISLALADGPTGPFAFHSVILPGAGGEAWDAHVTHNP
                     AVMEFEGRYYLYYMGNCGNGEFWNHRNNQRIGVAVANHPCGPWQRFDQPLIEVSKSSW
                     DYMITSNPTVCPTPDGRFLMIYKTCTNGPLPFGGDVYHAAAFADSPTGPFTRLPDPIF
                     TCEGVKFPAEDPCVWHQDNAYHVICKDMQGCFTDQGPGLVEFSSPNARDWSLAKHPFA
                     SPRQIIWEDGEVQPVALLDRPQVYVENGVPTTLSCAIKPDEGETETFNIQIPLAR"
     gene            1583..2131
                     /gene="amrA"
                     /locus_tag="VHS06_03485"
     CDS             1583..2131
                     /gene="amrA"
                     /locus_tag="VHS06_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX2987215.1"
                     /translation="MLNDQQRTRLLEVARQSVMAICCGDAEPDLTTDDPALTSVQGAF
                     VTLHRPDGDLRGCIGHIEGHLPLIETVAGMAVAAATQDPRFPRVRCEEVPGLHIEISA
                     MSPITEVKKTEDIEVGRHGLIVTCGYRRGLLLPQVATEYQWTCDEFLCHTCIKAGLPP
                     HAWKDEATKIECFEAEVFSEPR"
     gene            complement(2230..>3377)
                     /locus_tag="VHS06_03490"
     CDS             complement(2230..>3377)
                     /locus_tag="VHS06_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987216.1"
                     /translation="DDMQRHHVDSVWVVNLNIADLGPLAKKLEARKMTLVPALGELHY
                     NIEWRRNNWEYLEKESKRALQAAGESPAVIAWALCDEPRKNIVGEMEQFRQKFIEWGA
                     KQRPVTVTMWPDSPTYARETKFPLVCTDIYPFFADGNPNGPNPANVSKRWYRTQVEMT
                     AREAKETGKTPWIMPQAFCDVWGPWKYDAKGDMVILPGGVMHWRPPTVAETRWQLWSA
                     IGAGAQGFYWYVYEPCVKDNADKPAYTGATFPASLVEKQERALHATGGLVRPDGSATP
                     EYDAAGETFAAVEKLLPLLKGCVPVDIWPVQVSAPGWVGMLQNPASKRTFVAVVNDDC
                     EKAQELTLTTANPKLRDLRTGRLLRVKDGKALVRLEAGEGTVLEVAE"
BASE COUNT          591 a         1260 c          944 g          582 t
ORIGIN      
        1 ggcaacatcg aaagctacga ggacttcctc gacgagttgg cgcaggtctt cgccggctgc
       61 cagcgggtcc tcaagccgaa gcgctacctg gcggtggtcg tctcggattt ccgccacggc
      121 ccgcaattcg tgctcttcca cgccgacctg gccaaacgca tcgagcagat cggactcccg
      181 ctacgcggca tcacgatcct gctgcaggac aacaagaacc tctacccctt cggcgtccca
      241 aacgccttcg tctcaaacgt ccatcaccag tacatcctga tccatcaaaa gcccgcgtag
      301 aggtgtcggt aggacaggcg tcccgcctgt caggccccgg tggaggggcc gaatggggga
      361 cgttggggcc ccatccggcc cacggctggc tgcgacattg acggtgggac aggcatcctg
      421 cctgtcgcgg ggcctcagac aggcgagacg cctgtcccac cgaagacttc tatcccaaag
      481 agccggcggt ctacgaatgc tcaacctcca accagtcccc cgccacgcgc ttttcgccat
      541 gcccggctgg cacgtctggt gcgggacgat ctgccgctgt cccgttcccc tcgggctccc
      601 ctctcccgga gaccgtctgc ggtctcatgg gagaggggcc gggggtgagg gaagccgcta
      661 cgccctcctc ttctcccgct ggcccaccgc cacgaccttc aacggctggg tcacccactc
      721 ggagatcagc ctcgccctcg ccgacggccc caccggcccc ttcgccttcc acagcgtaat
      781 cctccccggc gccggcggcg aggcctggga cgcccacgtc acccacaacc ccgccgtcat
      841 ggagttcgag ggccgctact acctctacta catgggcaac tgcggcaatg gcgagttctg
      901 gaaccaccgc aacaaccagc gcatcggcgt cgctgtagcc aatcacccct gcggcccgtg
      961 gcagcgcttc gaccagccgc tcattgaggt gagcaagtcc tcgtgggact acatgatcac
     1021 ctcgaacccc accgtctgcc ccactccgga cggccgcttc ctgatgatct acaagacctg
     1081 caccaacggt ccgctgccgt tcggcggcga cgtctaccac gcggcggcct tcgccgacag
     1141 ccccaccggc ccgttcaccc gtctccctga cccgatcttc acctgcgagg gcgtcaagtt
     1201 ccctgccgaa gacccctgcg tctggcacca ggacaacgcc taccacgtta tctgcaagga
     1261 tatgcaaggc tgcttcaccg accagggccc cgggctcgtg gaattcagct cgcccaacgc
     1321 ccgcgactgg tcgctagcca aacacccttt cgcctcgccg cgtcagatca tctgggaaga
     1381 cggggaggtc cagccggtcg cgctgctcga ccgcccgcag gtgtatgtgg agaatggcgt
     1441 gccgacgacg ctgtcatgcg ccatcaagcc tgatgagggc gaaactgaga cgttcaacat
     1501 ccaaataccg ttggcccggt agcctatgtt cggattccgg tacgcccgac actattatcg
     1561 ggccccgtgg aggcagacaa ccatgctcaa cgaccaacag cgcaccagac tcctcgaagt
     1621 cgcccgccag tccgtcatgg caatctgctg cggcgatgcc gagcccgatc tcaccaccga
     1681 tgaccccgct ctgacctcag tccagggcgc cttcgtgacc ctgcacaggc ccgacggcga
     1741 cctcaggggc tgcatcggcc acatcgaagg ccacctgccg ctcatcgaga ccgtcgccgg
     1801 aatggcggtc gcggcggcca cgcaggaccc gcgcttcccc cgcgtccgtt gcgaggaagt
     1861 cccgggcctg cacatcgaga tctcggcgat gtcaccgatc acagaggtca agaagactga
     1921 ggatatcgag gtaggccgcc acggcttgat cgtcacgtgc ggctatcggc ggggcctgct
     1981 cctgccgcag gtagcgacgg agtatcagtg gacgtgcgac gagttcctct gccacacctg
     2041 tatcaaggca ggcctccccc cgcacgcgtg gaaggacgaa gcaacgaaga tcgagtgctt
     2101 tgaggcagag gtgttttcgg agccaagatg aggcatagcg gagtgtttcc gcgtggggcg
     2161 atcttccacg atcgccagtc cgcacgacgg cgatcttgga agatcgcccc acgcgaggaa
     2221 agccgatagc tactccgcca cctctagcac agtcccctcc cccgcctcca gccgcaccag
     2281 cgccttcccg tccttcaccc tcagcagccg acccgtccgc aggtcccgca gcttcgggtt
     2341 agcggtcgtc agggttagct cctgggcctt ctcgcagtca tcattcacga ccgccacaaa
     2401 ggtccgcttc gacgccggat tctgcagcat ccccacccag cccggcgcgc tgacctgcac
     2461 cggccagata tccaccggaa cgcagccctt cagcagcggc agcagcttct cgactgccgc
     2521 aaaggtctcg cctgccgcgt catactccgg cgtggcggag ccatccggcc gcacgagccc
     2581 gccagtcgca tgcagcgccc gctcctgctt ctccaccagc gaggccggga acgtcgcgcc
     2641 tgtgtaggca ggcttgtcgg cgttgtcctt cacgcacggc tcgtagacat accagtagaa
     2701 tccctgcgcg cccgcgccaa tcgcgctcca tagttgccag cgcgtctccg ccaccgtcgg
     2761 cggccgccag tgcatcactc cgcccggcag gatcaccatg tcgcccttgg cgtcatactt
     2821 ccacggcccc cacacatcgc agaaggcctg tggcatgatc cacggcgtct tccccgtctc
     2881 cttcgcctcg cgcgcggtca tctccacctg cgtgcgatac cagcgcttgg acacgttcgc
     2941 cgggttcgga ccattcggat tgccgtcggc gaagaagggg tagatgtccg tgcacacaag
     3001 cgggaacttg gtctcccgcg cataggtcgg cgaatccggc cacatcgtca ccgtcaccgg
     3061 gcgttgcttc gcgccccact cgatgaactt ctgccggaac tgctccatct cgccgacgat
     3121 gttcttgcgc ggctcatcgc acagcgccca cgcaatcacc gccgggctct cccccgccgc
     3181 ctgcagcgcc cgcttggatt ccttctccag gtactcccag ttattgcgcc gccactcaat
     3241 attgtagtga agctccccca gcgccggcac cagcgtcatc ttccgcgcct caagcttctt
     3301 cgccagcggc cccaggtccg cgatgttcag gttcaccacc cagacactgt ccacatgatg
     3361 gcgctgcatg tcatcca
//
LOCUS       DATALS010000162         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_373413, whole genome shotgun sequence.
ACCESSION   DATALS010000162 DATALS010000000
VERSION     DATALS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373413"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VHS06_03495"
     CDS             complement(<1..676)
                     /locus_tag="VHS06_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987217.1"
                     /translation="MTQTLSHLPGRLATFFSARSAARVAAGVALCSALLAASSGFAAE
                     SIRILTLDQGKNPRVTSDDQMVATRLHWDHGGVQMYIYSPQATVGMDTMTILPRNSNP
                     SGITDGAIIDALGNTLTAWNDVGAGFDFNSGIVPTRAGIPPSLTQFYDGAALDGYSII
                     SFQDPGISFGTGETTILGLTSLFYFQVDTDLSDLSNLPSFAIGSTLPDGSGAVVQIDI
                     NQDNIFD"
     gene            828..>2315
                     /locus_tag="VHS06_03500"
     CDS             828..>2315
                     /locus_tag="VHS06_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987218.1"
                     /translation="MDTDLSDLSNLPSFAIGSTLPDGSGAVVQIDINQDNIFDLRLPA
                     KFYPAGTIIEGDISMNQEIPWDLPPEDEGDIPEDQLPPNSNFPDIQGTVLHELGHVEG
                     LAHCYMVEPTMSGTPILPSTNPYDVRELDFDDKLALHLAYTSSPLTRLGKGGIQGRII
                     NGDAADNVSSTPLPIPEIENTPVWLGRPNDDGSYNSDDVVGVDETTSFTHKIRLFAEV
                     MNSPNFKRPLNMGDPFAGAAIVRDNRYSFGGLPPSSSPVFVNSNTVLPPNGYVLYVQP
                     APFLTNDEAQATFPPDSTLIPPEFYGGTTPYALIGIGDVADPNTPGDGYVQDNYLQFA
                     YNMLGQYALRIAGSQYQLLENAMTPSQGYITYRITTADGHTSDVANFAPSLDLVTTGI
                     VENDLDNYAQGTFVVGNAIVSTETLSLGHHRGDGLTSPTDMLVQTTIQNITTQPLQVG
                     VRFLSKPVIYTDDYLSFEVNGKLYRQEEEWVKDRTQNPNREPMPSI"
BASE COUNT          446 a          784 c          692 g          393 t
ORIGIN      
        1 ggtcgaagat attgtcctgg ttgatgtcga tctggacgac cgcgcccgag ccgtcgggca
       61 gcgtcgagcc gatggcaaag gaggggagat tcgacaagtc gctcaggtcc gtatccacct
      121 ggaagtagaa caggctggtc agacccagga tcgtcgtctc gccggtaccg aagctgatgc
      181 cggggtcctg gaagctgatg atgctgtagc cgtcgagtgc cgcgccgtcg tagaactggg
      241 tgagactggg gggaatcccg gcgcgggtgg gcacgatgcc tgagttgaag tcaaagccgg
      301 ctccgacgtc attccacgcg gtgagcgtgt tgccgagggc gtcgatgatg gcgccgtcgg
      361 tgatccccga gggattgctg ttgcgcggca gaatcgtcat cgtgtccatg ccgacggtgg
      421 cctgggggct gtagatgtac atttgcacgc cgccgtggtc ccagtgcagg cgcgtggcta
      481 ccatctggtc gtcggaagtg acgcgggggt tcttgccctg gtcgagggtc aggatgcgga
      541 tgctctccgc cgcaaagccg ctgctggcag ccagcagtgc cgagcaaaga gccacgcccg
      601 cggccactcg tgcggccgaa cgggcggaga agaaagtggc gagccttcca ggcaggtggc
      661 ttaaggtctg tgtcatctta gtttgcgaac cctctcacga gcgctttgaa ttcctcgaaa
      721 tcttgaaccg ggggcaggct gcccggggtg ttcagagcct gagaaaaccc ggcagtccgg
      781 ggcatgttgg ctttgggtgc ggcactcgca gccggttcta cttccaggtg gatacggacc
      841 tgagcgactt gtcgaatctc ccctcctttg ccatcggctc gacgctgccc gacggctcgg
      901 gcgcggtcgt ccagatcgac atcaaccagg acaatatctt cgacctgcgc ctgcccgcca
      961 agttctaccc ggccggcacg atcatcgagg gcgacatctc catgaaccag gagatcccct
     1021 gggatctgcc tccggaagat gagggcgaca tccccgagga ccagcttccg cccaactcga
     1081 atttccccga catccagggg accgtgctcc acgagctggg acacgttgag ggcctggctc
     1141 actgctacat ggtcgagccg acgatgagcg gcaccccgat cctgcccagc accaatccct
     1201 acgacgtgcg cgagctggat tttgacgaca agctggccct gcacctggcc tacacctcca
     1261 gtccgctgac ccgtctgggc aagggcggca tccagggacg catcatcaac ggcgacgcgg
     1321 ccgacaacgt ctccagcact cctttgccga tccccgagat cgagaacacc ccggtgtggc
     1381 tgggcagacc caatgacgac ggcagctata actccgacga cgtggtcggc gtggacgaga
     1441 caacctcgtt cacccacaag atccgcctgt ttgcggaggt gatgaacagc cctaacttca
     1501 agcgcccgct caacatgggc gatccgttcg ccggcgcggc catcgtgcgc gacaaccgct
     1561 actccttcgg cggcctgccg ccctcgagca gcccggtctt cgtcaacagc aacaccgtgc
     1621 tgccccccaa cggctacgtg ctctacgtgc agcccgcccc gttcctgacc aatgacgagg
     1681 cccaggccac cttcccgccc gactcgacgc tcatccctcc ggaattctac ggcggaacga
     1741 ccccctacgc cctgatcggt atcggcgacg tcgccgatcc caataccccg ggcgacggct
     1801 acgtccagga caactacctg cagtttgcct acaacatgct cggccagtat gccctgcgca
     1861 ttgccggctc gcaataccag ctgcttgaaa acgcgatgac cccctcgcag ggctacatca
     1921 cctaccgcat caccacggcc gacggccaca caagcgacgt ggccaacttc gccccgagcc
     1981 tggacctggt cacgaccgga atcgtggaga acgacctgga caactacgcg cagggaacct
     2041 ttgtggtcgg caacgccatc gtctcgaccg agacgctctc cctgggccac caccgcggcg
     2101 acggcctgac cagccccacc gacatgctcg tgcagaccac gatccaaaac atcacgaccc
     2161 agcccctgca ggtcggcgtg cgcttcctga gcaagccggt catctacacc gacgactacc
     2221 tctccttcga ggtcaacggc aagctctacc gccaggagga ggagtgggtc aaggaccgca
     2281 cgcagaatcc caaccgcgag cccatgccca gcatc
//
LOCUS       DATALS010000163         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_196644, whole genome shotgun sequence.
ACCESSION   DATALS010000163 DATALS010000000
VERSION     DATALS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196644"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VHS06_03505"
     CDS             <1..560
                     /locus_tag="VHS06_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2987219.1"
                     /translation="LPAEFVTPQVEPERRTVPATRGDPWISLTTRMIPLAPLMVFVVI
                     GVQAGLRLVINQPAPGAAYELHREYSSEPVGLTLDRTDAAAREAFAAAAAIQATPVSA
                     RGSYPSGHASRVLFFSLLAAQTVRRRGKVSRATRTAVVVLPTALVGYSALYFGYHWPS
                     DLLGGTLLALAIYAVVVHLAAGIRR"
     gene            664..>2080
                     /locus_tag="VHS06_03510"
     CDS             664..>2080
                     /locus_tag="VHS06_03510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2987220.1"
                     /translation="MELSFTVIIPARNAASVLGKCLSSVVSKGAGGSPLEVIVVDDGS
                     TDDTVAVAKSHGARVVYTGGLGPAAARNAGARVASGDLLVFLDADCVAEQGCLEALLA
                     PFADPAVAGSRGGYTSDQEALLARFVQLEMDEKQARLEKSGLVTLLDTACAAYRRTLF
                     TDQGGFDERLPATSVEDAELSFRLTAQGYHLVYAPSARVRHRHPEQLGNYLWRKLRYG
                     YFRALLYRRYPARVRNDGYTPRLMPVQILLAGIVMAAGGLGIWSTAGRTIAAAAAMLF
                     LLASVPLSRRAWGSDHRLAMATPFLLLLRSCAQGLGLLAGVAQLAKGSRSTRWVGGAL
                     TIVSLATLALLVLRSDWQQLIRPLAEARPEWLAVAIGTAFAVEVAKTVRWQLLLGLTF
                     GDLPRLLALVFTGRLLNVLAPLRAGDLWRVASAATSEGQPVLVAGGSVVAEKALDGAA
                     LAAAGIVLVWLPRHTEGWILLP"
BASE COUNT          325 a          687 c          662 g          406 t
ORIGIN      
        1 ccctaccagc cgaattcgtc actccccaag ttgaacccga gagaaggacg gtgccggcaa
       61 cccggggaga tccctggatt tcccttacga cccgtatgat ccccctcgcc cctctgatgg
      121 tctttgtcgt gataggagtg caggccgggc tgcgcctggt gatcaaccag cctgccccag
      181 gcgccgccta cgagttgcac agagagtact cttcggaacc cgttgggctc accctggacc
      241 gcaccgacgc ggccgcccgc gaggcatttg ccgcggccgc ggcgatccaa gccactcctg
      301 tctcagcaag aggcagttac cccagcggcc acgcatcgag ggtgctcttc ttttcccttc
      361 tcgctgccca aacagttcgt cgtcgaggga aggtctcacg agccacgcgg acagcggtcg
      421 tggtgttgcc caccgccctg gtcggataca gcgccctcta cttcggctac cactggccga
      481 gcgacctgct gggagggaca cttctggcgc tcgcgatcta cgcagtagtg gtgcatcttg
      541 ccgcaggaat tcggcgctga tgccttctaa gtaagtccct ttctgctacc tacctagtat
      601 cctgttgtag ctggtataat gggtttgttc tactctccca cccagattcc ctgagcgaaa
      661 gctgtggaat tgagcttcac cgtaatcatc cccgcgcgca atgcggcctc cgtcctcggc
      721 aagtgcctgt cgtccgtagt gtccaaaggg gccggcggtt cccctctgga ggtaatcgtg
      781 gtggacgacg gctccaccga tgacaccgtg gccgtggcga aatctcacgg cgcccgtgtg
      841 gtatataccg gtggtctggg cccggctgcg gccaggaatg cgggcgcgcg agtcgcctcc
      901 ggcgatctgc tggtcttctt ggacgccgat tgcgtcgcgg agcagggttg cttagaggcg
      961 ctgctggcgc cattcgccga ccctgccgtg gcagggagcc gcggcggata caccagcgac
     1021 caggaggcgc tgctggccag gttcgtacag ctcgagatgg acgagaagca ggctcgctta
     1081 gaaaagagcg gactggtgac gttgctggac actgcctgcg ccgcctatcg gcgcaccctc
     1141 ttcacggacc aggggggctt cgacgaaagg cttccggcaa cctccgtcga agacgccgaa
     1201 ctctcatttc gactcaccgc ccaggggtac catctggtct acgccccctc cgccagggtg
     1261 cgccaccggc accccgagca gttgggcaac tatctctggc ggaagctccg ctacggctac
     1321 ttcagggccc tgctctaccg tcgctatccg gcgcgagtgc ggaatgacgg gtacactcca
     1381 cggctgatgc ccgtccagat cctactggcg gggatcgtga tggccgcagg cggcctcggg
     1441 atctggagca ccgccggaag gactatcgcg gccgcagccg cgatgctctt tctgctggcg
     1501 tcggtcccgt tgtcccgcag ggcatggggt tcggaccacc gcctggctat ggcaaccccg
     1561 ttcctcctgc tgcttcgatc ctgcgcccag gggcttggac tgctggccgg cgttgcgcaa
     1621 ctggccaaag gatcccgatc gacacgctgg gtaggcggcg ccctgaccat agtctccctg
     1681 gcgacccttg cgctgttggt cttgcggtcc gactggcagc agttgatccg gcctctcgcc
     1741 gaagcacgcc cagagtggct ggccgtggcg atcggcacgg ccttcgccgt ggaggtggcg
     1801 aagaccgtcc gatggcagtt gctgctgggt ctcaccttcg gcgatcttcc tcgcctgctg
     1861 gcactggtat tcacgggcag actgctaaac gttctggcgc ccctgcgagc gggagatctc
     1921 tggagggttg cctcggccgc aactagtgag ggacaacccg tgctggtcgc cggaggatcg
     1981 gtggtagcgg agaaggcgct ggacggcgcc gcgctcgccg ctgccggcat tgtgctagtc
     2041 tggctgccgc gccatacgga gggctggatc ctactgccct
//
LOCUS       DATALS010000164         5313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_277747, whole genome shotgun sequence.
ACCESSION   DATALS010000164 DATALS010000000
VERSION     DATALS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277747"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VHS06_03515"
     CDS             complement(<1..953)
                     /locus_tag="VHS06_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2987221.1"
                     /translation="MAQRSRRATGKLPPDPEPRGTSISSMPGSTQPDQARLYLAAIVE
                     SSEDAIIGLTMDGLITSWNPAAERLYGYCAEEVLGKPISILVPPSRPNELEPLVERLS
                     RGEGVQHYETVRARKDGSLVDVSVSVSAIRNGEGKIVGIATIARDVSERRRMEEVLRS
                     SEANYRAIYDAASDAIFVIDPQTHLVVDVNRRLMEMYGGYTPDETRNLNIGTFSLGEP
                     PYDQENAARLVEKAAAGEPQLFEWLARGKTGRLFWVEVSLKRATIGGKTRVLAVVRDI
                     TERKRVEEERERLLAEVQARAGELDATIASIADGVLIYGPSG"
     gene            complement(1545..3458)
                     /gene="feoB"
                     /locus_tag="VHS06_03520"
     CDS             complement(1545..3458)
                     /gene="feoB"
                     /locus_tag="VHS06_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX2987222.1"
                     /translation="MHGHHGHHGSCHRGGAATLELAHQHHPGQGGRHGADGRWIVLVG
                     NPNTGKSVFFNALTGIYVDVSNFPGTTVDVSQGRWGNDTVLDTPGIYGVAAFNDEERV
                     AREIIVQADVVVNIVDAVHLERDLFLTQQLIDMGLPLVVALNMMDEAEAQGLRIDVAM
                     LEESLGVPVIPTVAVKQQGLDRLKSAIPEARRGHIDPTLSTKLRSWYGTEETTPAMLL
                     AAEGDEEIAAAADLQPMYAREELYLHRRQRVNDLLARAVADTRDEGGFGETLSRLMIR
                     PLTGVPILGGVLVLLYFLIGNLIAQQVVGFTEGTVMAGIYEPLVRGVVQRVLDPASPL
                     SVLLVGEFGLITMTVTYIVGLLLPLVIGFYLALAIMEDSGYLPRLATLVDRALTGFGL
                     NGRAIVPMILGLGCVTMATMTTRILGSQRERRIATALLGLTIPCSAQLAVITAMLAGL
                     GPAYAAFYTFIIVAILVAVGTALNLLLPGKSTGLLIDLPPLRVPRPVNVLKKTYGKVQ
                     MFLSDAGPLFALGSVLLSVLQITGGLQGVQDLLAPLTVGWMQLPKEASTAFVMGFVRR
                     DFGAAGLLTMPLTSMQTLVALVTLTLFVPCIASVMMLFKERGWKEAAVVWVGCLVAAF
                     VVGGVLSQLLIRI"
     gene            complement(3458..3676)
                     /locus_tag="VHS06_03525"
     CDS             complement(3458..3676)
                     /locus_tag="VHS06_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEX2987223.1"
                     /translation="MTLDMVHRGDSIRILKIEDPQVRFQAIRFGIGEGTDVRCAEKLP
                     AGPIVLRRGRQEIAIGRAIARTISVEAL"
     gene            complement(3815..4297)
                     /locus_tag="VHS06_03530"
     CDS             complement(3815..4297)
                     /locus_tag="VHS06_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX2987224.1"
                     /translation="MEPPQQLSVTARQAINTTGRRMTPQRELLLEIIQQSDEHLDAEE
                     IHRRARDRGERISLSTVYRTLGLLKELQLVDELHLWDEHHHYESRTTQEHVHLLCRVC
                     GAVTEISGAVVEEMKALASRQHSFSIERAEIEFVGLCQECRERVAQTGGAQEMAAERG
                     "
     gene            complement(4576..>5313)
                     /locus_tag="VHS06_03535"
     CDS             complement(4576..>5313)
                     /locus_tag="VHS06_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX2987225.1"
                     /translation="IGVIVETIDDIAEQTNLLALNAAIEAARAGEHGKGFAVVADEVR
                     KLAERSSRSTKDIAALIAEVQKGTSEAVAAVERESKEVEAGSRLAEEAGEALDRTLAS
                     VKVAADQASHIASAVLEMETISRQVVALMDSVSAVVAQSAAATEEMAASSQEVEGAMK
                     KMASVSEEASASAKEVTASSQEMGAQVASIADQAQDLARMAEELRTAVSQFKTEDGGS
                     GSTEVVSRRRKSDWAANRPDATVRSAD"
BASE COUNT          988 a         1794 c         1546 g          985 t
ORIGIN      
        1 cccgacggac cgtagatcag gacaccatcg gcaatcgatg cgatagtagc gtccaattcc
       61 cccgcacggg cctgaacctc cgccagcaat cgctcccgct cctcttcaac ccgcttacgc
      121 tcggtgatgt cccggacgac cgccagtact cgggtcttgc cgcctatggt tgcacgcttc
      181 aagctcacct ccacccagaa caaccggccc gtcttccctc tagccagcca ttcgaatagc
      241 tgaggctccc ccgcggcggc cttctcgacg agccgagcag cgttctcttg atcgtatggc
      301 ggctcgccca ggctgaaggt gccgatattg aggttccggg tctcgtccgg ggtgtatccg
      361 ccgtacatct ccatgagcct gcggttgacg tcgacgacca ggtgggtctg gggatcgatg
      421 acgaagatgg cgtcgctggc cgcgtcgtag atcgctcggt agttggcttc cgacgaccgc
      481 aacacctctt ccatccgcct tcgttccgat acatcacgag cgatcgtggc aatccccacg
      541 atcttgccct caccattcct gatggccgaa acgctcaccg agacgtccac caggctgccg
      601 tccttcctcg ctcgaaccgt ctcgtagtgc tggacccctt ccccccgact gagtctctcc
      661 acgaggggtt ccaactcatt cggccggctc ggcgggacca ggatggagat cggcttgccc
      721 aggacctcct cggcacagta gccgtagagt ctctcagccg cggggttcca gctagtgatc
      781 agaccgtcca tggtcagccc gataatggca tcttctgaag actccactat ggcagccagg
      841 tacaacctgg cctggtccgg ctgcgtggag ccgggcatgc tggagatcga agtaccacga
      901 ggctcggggt caggtggcaa cttgcctgta gcgcggcgac ttcgctgtgc catcaccctc
      961 tcctcgttgc ggaagactgc ggagaaaagc aactacagat gccaatcaga tatttcgcaa
     1021 ggacggtgcc atgcgggatc gcaggcctag cctgaggcta cactactcac ctgagagcac
     1081 aatacatctt catgactcaa gagaggggtt cggcgctcac agcgcagtcc cggcggcgtc
     1141 agtggggtcg aggtctacgg tctgagcgag ggaagatgca gtccacagcc gctacagccg
     1201 ccgcagttaa tggcggggag ggccgaccag cagcgcgggc agcgacccac cataggatcg
     1261 gtgacggcgt aaccacactg ctcgcaccta cgtagcccac aagcacagtc ggagggagga
     1321 gcactggtct caggtttctg ttcttgtcta tcagtcatcc tacaccacct ttggttcagt
     1381 gccgaggtgc aacacctctt tggagtgcgg cggcttgacg ccgcaacgtt gccccgcaca
     1441 ccttgccgat cggagatggc cttcctatcc tggaagcgcc cagcacttcg gggtcggctt
     1501 acgcctcccc ccacgaaagc ggcgctcacg cgccgcactc cacactatat ccggatcaac
     1561 aactgggaca acacgccgcc gactacgaag gccgccacca ggcagcccac ccataccacc
     1621 gcggcctctt tccagccccg ctccttgaac agcatcatca ccgaggcgat gcagggcacg
     1681 aagagggtca gggtaaccag cgccaccagc gtctgcatgg aggtcagcgg catggtgagc
     1741 aggccggccg cgccgaaatc cctccgaacg aaccccataa cgaaggcggt ggacgcctcc
     1801 ttgggcaact gcatccaccc caccgtgagg ggcgccagca gatcctgcac cccctgcaac
     1861 ccgccggtga tctgcagcac gctcagcaga acggagccca gggcgaagag ggggccggcg
     1921 tcggagagga acatctgcac cttgccgtag gtcttcttca gcacgttgac cgggcgaggc
     1981 acccgtaggg gcggcaggtc gatcagaaga cccgtggact tgccgggcag cagcaggttc
     2041 aaagcggtcc ccaccgccac aagaatggcg acgatgatga aggtgtagaa ggccgcgtag
     2101 gccggcccca gcccggccag catggccgtg atcaccgcca attgggcgga gcaggggata
     2161 gtcagcccca gcagcgccgt ggcgatcctc cgctcacgct gagagccgag gatgcgggtg
     2221 gtcatagttg ccattgtgac gcagccgagc ccgaggatca tcgggacgat tgcccggccg
     2281 ttgaggccga agccggtgag cgcccggtcc accagggtgg cgagacgcgg caggtagccg
     2341 gagtcctcca tgatggccag cgccaggtag aagccgatga ccagcggcag cagcagtccc
     2401 acgatgtagg tcaccgtcat ggtgatcaag ccaaactcgc cgaccagcag cacgctcagc
     2461 ggcgaggcag ggtccaggac gcgctgcacc accccccgca ccaaaggctc gtagatccca
     2521 gccatcaccg tcccctcggt gaagcccacg acctgttggg cgatcaggtt tccgatgagg
     2581 aagtagagca aaaccagcac accgcccagt atgggaactc cggtcagtgg gcggatcatc
     2641 agccggctca acgtctcgcc gaagccgccc tcgtcgcgag tgtctgccac cgccctcgcc
     2701 agcaggtcgt tcacccgctg ccgtcggtgc agatacaact cctccctggc gtacatgggc
     2761 tgcaggtcgg cggcggctgc gatctcctca tcgccctcgg cggccagcag catcgcgggg
     2821 gtagtctcct cggtgccgta ccaggaacgc agcttggtgc tcagggtggg atcgatgtgg
     2881 ccgcggcgag cctcgggaat ggccgacttc agtctgtcga gcccctgctg cttcactgcg
     2941 acggtgggga tcactgggac cccaagcgac tcctccagca tggcgacatc tatcctcagc
     3001 ccctgggcct cggcctcgtc catcatgttc aacgctacca caagggggag ccccatatcg
     3061 atcaactgct gggtgagaaa gaggtcgcgc tccaggtgga cggcatcgac gatgttcacc
     3121 accacgtcgg cctggacgat gatctctcgg gcgacccgct cctcgtcgtt gaacgcggcc
     3181 acgccataga tgcccggtgt gtccaggacc gtatcgttgc cccagcgtcc ctgggaaacg
     3241 tcgaccgtgg tgcccgggaa gttggagaca tccacgtaga ttccggtgag ggcgttgaag
     3301 aagacggact tgccggtatt ggggttgccg accagcacaa tccaccggcc atcggcccca
     3361 tgtcgcccac cctgtccagg atggtgctga tgggccaact cgagggtggc cgcgccaccc
     3421 cgatgacaac tgccgtgatg tccatgatgc ccgtgcatta cagtgcctcg acgcttatag
     3481 tcctggcgat cgcgcgtccg atggcgattt cctggcggcc acgccgaagg acgatgggac
     3541 cggcggggag cttttcggca cagcggacgt cggtgccctc gccgatgcca aagcggatgg
     3601 cctgaaaacg aacctgcggg tcctcgatct tgaggatacg gatggaatcg ccgcggtgaa
     3661 ccatatcgag ggtcatgatc ggatcctccc tgaacggcgg aggcggcctc agcgacggga
     3721 gcgccccgct gtctaaccct gctccactct actgcaattg caaatcattt gcaatacgat
     3781 ggtcaaagaa gagaggcctc gggcgcatcc gcccttagcc ccgctccgcc gccatctcct
     3841 gtgccccgcc ggtctgagca accctctccc ggcactcctg gcacagtccc acgaactcta
     3901 tctccgcacg ctctatggag aagctgtgct gccgagaggc gagcgccttc atctcctcca
     3961 ctaccgcccc ggagatctcc gtcaccgcac cgcagacgcg gcacagcagg tggacgtgct
     4021 cctgggtagt tcgcgactcg tagtggtggt gctcgtccca gaggtggagc tcgtccacca
     4081 actgcaactc cttcaagagc cccagggttc gatagacagt ggagaggctg atccgttccc
     4141 ccctatccct cgccctgcgg tggatctcct cggcatccag atgctcgtcc gactgctgga
     4201 tgatctccag cagaagctcc cgctgggggg tcatccttcg acctgtggtg ttgatagctt
     4261 gtcgtgccgt gacgctgagc tgctggggag gctccatcga ctcacctaac tgcgaacgat
     4321 ttgcaattgc aattgtacca ttgtgcgcga agttgtcaag cggtttggca cttcactcca
     4381 ccacaacaat acaaagtcgt ccattcggca tccatgtcgc catcggcaaa tggcaatcct
     4441 gcctgagcgg caccagcgag tggcaacgcc tgcatcaaac gcccctctaa gcggcttccc
     4501 cttgttggta tgccttttcc cctcaaggaa acgttgactg catccaggga aagccgctta
     4561 gacactggcc ttccttcagt cggccgacct tacggtggca tccggccggt ttgcagccca
     4621 gtcgctcttg cgtcgacggg agaccacctc ggtgctaccg ctcccgccgt cctctgtctt
     4681 gaactgggac acggcggtac gtagctcctc tgccatccgt gcaaggtcct gggcctggtc
     4741 cgcgatactc gccacctgag cacccatctc ttgacttgat gcagtcacct ccttcgccga
     4801 ggcgctggcc tcctcactga ccgatgccat cttcttcatc gcgccttcga cttcctggct
     4861 cgaggccgcc atctcctcgg tcgccgccgc cgactgcgca acaacggcgg atacggagtc
     4921 catcagtgct acgacctgcc tggagatggt ttccatctcc aggactgccg aggcgatgtg
     4981 cgatgcctgg tctgcagcta ccttcacaga agcgagagtc ctatcgagtg cttcgcccgc
     5041 ttcctccgcc agtctgcttc ccgcctccac ttccttggat tctcgttcca cagcagccac
     5101 ggcctcgctc gtgcccttct ggacctcggc tatgagagca gctatatcct tagtggagcg
     5161 ggacgatctc tcggcaagct tcctcacctc gtcggccacc accgcaaaac ctttgccgtg
     5221 ctcccctgct cgagcagcct cgatcgccgc attcagggca agaaggttgg tctgctcggc
     5281 gatgtcgtcg atggtctcga ctattacccc gat
//
LOCUS       DATALS010000165         6066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_150344, whole genome shotgun sequence.
ACCESSION   DATALS010000165 DATALS010000000
VERSION     DATALS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150344"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..426)
                     /locus_tag="VHS06_03540"
     CDS             complement(40..426)
                     /locus_tag="VHS06_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987226.1"
                     /translation="MDAGFDARELSQGEIDALLSKLSSGEVVESEEEKPVDPRWKVVK
                     TYDFRRPDKLSKDQMRTLQLIHESFGRLVSSSLSAYLRTAVQFNLVSIEQGVYGEYVE
                     RVPPDSSLASKPASISTYHPFYCVTN"
     gene            complement(445..966)
                     /locus_tag="VHS06_03545"
     CDS             complement(445..966)
                     /locus_tag="VHS06_03545"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX2987227.1"
                     /translation="MRLPRGSIIGIAAGVGAAVVVVGAFIIGSAVGGGKAAANEANTG
                     REGKAAAPATMAPGPMFQMKETVVNLSDQGGRRYLKIALSVEFTANADEFRKANAEER
                     KAKQAEFDKKLAPQQPLIEDTIITVLASRTTTDVASPEGKQQLKADLKDSLNRALGGD
                     QVANIYFSQFVTQ"
     gene            complement(947..1795)
                     /locus_tag="VHS06_03550"
     CDS             complement(947..1795)
                     /locus_tag="VHS06_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX2987228.1"
                     /translation="MASQAVPDRKHRVKPKKENSERWLLTYSDMMTLLLAFFVIMYGM
                     SNADAAKFERLASGMKKAFNVGILTGSPDAAMMEQTMDLIDSSGGADQTAVEGKDMEM
                     IFSEMGWALDDEALSDKVSVNVRPEGIAISLSGNLLFSSGRAELRPDAVKLLHSLGRV
                     LSRVPNSVRIEGHTDDIAPSGTGYPTNWELSGARAVAVVRYFTEMEGINPQRLSAVAY
                     SQYQPVSANDSARSRARNRRSEILILYPPSQTTARAAAAERTPTGGPGSVASSERESN
                     SAVATR"
     gene            complement(1749..2573)
                     /locus_tag="VHS06_03555"
     CDS             complement(1749..2573)
                     /locus_tag="VHS06_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HEX2987229.1"
                     /translation="MNLTTIIGLVVGFGSLMVSLVMEGGDLLSFLKPSAALIVFGGTF
                     GATFISFPGSLIARFPKIVMRAFFNKSVRVSELADVLVNLSERARREGLLALEEELAG
                     IENPLLKKGIMLVVDGTDPDLVRGVLESDIAVREREAEGESGMLEAMGGFAPTMGIIG
                     TVMGLVNVLAKMDDPSKLGESIAVAFIATFYGIGSANLMWLPFGTKLKKITESEQVLD
                     EMILEGVASIQSGENPRILQERLSPFLTGEKKGKEAKEQEGDGIAGSARQEAPGQA"
     gene            complement(2570..2830)
                     /locus_tag="VHS06_03560"
     CDS             complement(2570..2830)
                     /locus_tag="VHS06_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HEX2987230.1"
                     /translation="MIRVSRLDGSELTINSDLVEAIEGTPDTVISLVNGHKLVVKETV
                     DVVVDRIVEFRRRINRCPEIRERIASFPISDSARNPSEGVDQ"
     gene            complement(2837..3571)
                     /locus_tag="VHS06_03565"
     CDS             complement(2837..3571)
                     /locus_tag="VHS06_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2987231.1"
                     /translation="MSSAEMVATPVVTQSVMHTFQPRRVPSGIRLFIADDHALFRQSL
                     KTVLEMDGGFTVVGEASDGREALHAIEQCSPDIALIDFGLPLLNGLEVCRRLRRTRSR
                     TRVVVLASRGQESLLLRTMDGGVAGYLLKESDLQELTVALKKVNAGFSYLTPSLEGKP
                     LDLYLRRMRSSDPTQSPDLLTSRERELLQLVGEGYTNREIADQLCLSVKTVEAHESNI
                     CAKLNVRGRAGLVRHAIHASMIGIDG"
     gene            complement(3939..4604)
                     /locus_tag="VHS06_03570"
     CDS             complement(3939..4604)
                     /locus_tag="VHS06_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2987232.1"
                     /translation="MKKARVMIADDHTLFREGLRQLLEMERDIEVVGEASDGMEAVAK
                     ARTLAPDVILMDINMPVVGGVAATEQILSERAETGIIVLTMHRQDQYVFDAMRAGARG
                     YLLKDAKPADLIFAIRMVARGASLVDPRMTTTVLKEFRRLASQVEPDQGVAGLTPKEV
                     EILKLLAMGLSNKEIGRELCLAEKTVKNYLSTVFQKLQINDRVQAAIYALKHGLLTEQ
                     EIS"
     gene            4788..5939
                     /locus_tag="VHS06_03575"
     CDS             4788..5939
                     /locus_tag="VHS06_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2987233.1"
                     /translation="MAASQHSLSEEEAFVSREMISLASEILSIDEACHTVLSLFARVF
                     PCVGSWMATTRTPNGALQLRATSGFSWTGEADLSALSLQRLAEIPSVETVLWVFDLEH
                     SPTVLPFVDEDVGAVVVATLSAHNGCVGIVALMLPTPWSFTPHQRKLLASLSNQAALV
                     VHSAILVAAAQELAIQEERSRIATEIHDGLSQNLALLMLKVEIISRLIDRDPERAKAE
                     LEKIQSIVELSVQELRHSINTLRSPDSAGLGLIPALQRMAGDFTKRTGINVELALPSD
                     LSLPPQAISTILGVVQERLGTASRQASASRVTVAVATAGDCLTVRIADDGHASVNPPL
                     DEDGATVPLLDRLRDLVRPLGGTVSTTADSTGMVVESRIPLRQCTTPSH"
BASE COUNT         1210 a         1929 c         1627 g         1300 t
ORIGIN      
        1 ggcgcttggt ggagatcagg ttgccaacat ctacttcagt cagttcgtga cgcagtagaa
       61 cggatggtat gtactgatgg acgcaggctt tgatgcaagg gagctgtcgg gcggaaccct
      121 ttctacgtac tctccgtaca caccctgctc gatggaaacc aggttgaact gaacagctgt
      181 ccgcaggtag gccgagagag acgaactgac cagtctgccg aaggactcgt ggatcaactg
      241 cagggtacgc atctggtcct tcgagagctt gtcggggcgt cggaagtcgt aggtcttcac
      301 caccttccaa cgaggatcga ccggcttctc ttcttcagac tcgaccactt ctccagagga
      361 gagcttcgaa aggagagcat ctatctctcc ctgcgacagc tcccttgcat caaagcctgc
      421 gtccatcagt acataccatc cgttctactg cgtcacgaac tgactgaagt agatgttggc
      481 aacctgatct ccaccaagcg ccctgttcag actatccttc aggtccgcct tcaactgctg
      541 cttgccttcc gggcttgcga catccgtcgt ggttctggag gccaataccg tgataatggt
      601 gtcctcgatc agcggttgct gaggagcgag cttcttgtcg aactcggcct gtttcgcctt
      661 tcgctcttcg gcattggcct tccggaactc atcggcattc gcggtgaact ctacggacag
      721 ggcgatcttc aggtagcgcc gccccccctg gtccgacaga ttcaccaccg tctccttcat
      781 ctgaaacata ggccccgggg ccatcgtcgc cggcgcagct gctttgcctt cgcgccctgt
      841 gttcgcctcg tttgcagcag ccttaccgcc gcctacggcc gatccgataa tgaaggcccc
      901 gacaaccacg accgcggctc ctacaccagc cgcgatcccg atgatgctac ctcgtggcaa
      961 ccgcactgtt actctccctc tccgatgatg ccaccgaccc tggcccccca gttggcgtgc
     1021 gctcggccgc tgcggcccgc gctgtagtct gggatggcgg gtagagaatc aggatttccg
     1081 accgacgatt cctcgctcga gacctcgccg agtcgttggc ggacaccggc tggtactgcg
     1141 agtaagccac ggccgacagc cgctgagggt tgataccctc catctctgtg aagtacctca
     1201 caaccgcgac tgccctggcc ccggatagct cccagttggt tggatacccg gtcccgctgg
     1261 gtgctatgtc atcggtgtgt ccctctatcc gcaccgagtt cggcactcgg ctgagcaccc
     1321 tgcccaacga gtgcagcagc ttgacggcat ccggcctcaa ctctgctcgg ccggaactga
     1381 agagcaggtt gcccgatagg ctgatggcta tcccctccgg gcgaacattg acactcacct
     1441 tatcggacag ggcttcgtcg tccagagccc accccatctc gctgaagatc atctccatgt
     1501 ccttgccttc gacggccgtc tgatcggccc cgcccgagct atcgatcagg tccatcgtct
     1561 gctccatcat tgccgcgtcc ggcgaaccgg tgaggatccc aacgttgaac gctttcttca
     1621 tcccgctcgc cagtctctcg aacttggcag catcggcgtt tgacatgcca tacatgatga
     1681 cgaagaaggc tagaagcagc gtcatcatgt cggagtaggt gagaagccag cgctcggagt
     1741 tctccttctt aggcttgacc cggtgcttcc tgtctggcac tgcctgcgat gccatctccc
     1801 tcctgctcct tcgcttcctt ccccttcttc tcgcctgtca ggaaggggga cagccgctcc
     1861 tgcagtatcc tggggttctc gccggattgt atcgaggcaa cgccctccag gatcatctcg
     1921 tccaggactt gttcggactc agttatcttc ttcagcttgg tgccgaacgg aagccacatc
     1981 aggttggctg atccgatccc gtaaaaggtg gcaatgaacg ccaccgcgat ggactccccc
     2041 agcttgctcg gatcgtccat cttggcgagc acgttcacca gtcccatcac tgtaccgatg
     2101 atccccatgg tgggggcgaa cccgcccatc gcctcgagca tgcccgactc gccttcggct
     2161 tccctctccc gaacggcgat gtcggactcc aggacgccgc gaacgaggtc ggggtcggtg
     2221 ccgtcaacca ccaacatgat ccccttcttc agcagtgggt tctcgatccc ggcgagttcc
     2281 tcctccagtg ccaggagacc ctctcgtcga gcacgctcag agaggttgac cagcacatct
     2341 gccaactcgg acaccctgac ggacttgttg aagaatgccc tcatgacgat cttgggaaac
     2401 ctggcaatca ggctaccggg aaagctgata aaggtggcgc cgaaggtacc gccaaacacg
     2461 atcagcgcgg cagatggctt caagaaagat agaagatcgc ccccctccat tacgagtgag
     2521 accatcagag agccgaagcc gactaccagg ccgataatcg ttgttaggtt cactggtcaa
     2581 ccccttcaga cggatttcga gccgagtcac ttatggggaa gctcgcaatc ctttcacgga
     2641 tctcagggca gcggttaatc ctccgcctga actccacgat gcgatcgacg accacatcga
     2701 ctgtctcctt aacgaccaac ttatggccgt tcaccaggga gatcaccgtg tccggcgttc
     2761 cctcgattgc ctccaccagg tcgctgttga tcgtgagttc agagccgtcc agccgactga
     2821 cgcgtatcac ggcggcctac ccgtcgatcc cgatcatgct tgcgtgaatg gcatggcgga
     2881 ccaggccggc gcgccctcgc acgttcaact tggcacagat gttcgactcg tgcgcttcaa
     2941 cagtcttgac actcaggcac agctggtcgg cgatctcacg attggtatag ccctcaccaa
     3001 ccaactgcag aagctccctc tccctgctgg tgagaagatc cggcgactgg gtcggatcgg
     3061 agctccgcat tcttctcaga tagaggtcca acggcttccc ctccaggctt ggggtcaaat
     3121 aggaaaagcc ggcattgacc ttcttcagtg caaccgtcaa ctcctggaga tccgactcct
     3181 tcaggagata cccggccacg ccgccgtcca tcgttcgcag aagaagcgat tcctgacctc
     3241 gtgaggccag cacgacgacc ctggtgcgcg acctcgttcg ccggaggcgc ctgcacacct
     3301 cgagaccgtt cagtagagga aggccgaagt cgatgagcgc gatgtcggga gagcactgct
     3361 cgatagcgtg cagcgcctct cttccatccg acgcctcacc gaccacagtg aacccgccat
     3421 ccatctccag caccgtcttc aaggactggc gaaacagggc gtgatcatcg gcgatgaaga
     3481 gccggatccc tgaaggcaca cggcgaggtt ggaacgtgtg cattaccgac tgggtcacca
     3541 ctggcgtggc aaccatttct gcgctactca aggccaacct cctgctccac agcttgcgac
     3601 gcatcgacac tcaatgagga ttcgtcgggg gattttgcga acaccgatac gatagtgtgg
     3661 aacagaaagt tcgatcaggg cccatggtcc caggtattgc ggaccaagcg gcacgtgaaa
     3721 acgcgcagaa agtcacacgg aatgggggac aaacggggag ttaggctgat gtagggcggg
     3781 ccgaaagacc actctcgcgc gttcggaccc gcatttggag ttgcccgagg cacccatcta
     3841 tctacagtaa cgggaccggg ggaattctgt tacagacttc gacagactgt tttgggacgg
     3901 ccccaagttc cggtgatggg gccacggacc accatggatc aggagatctc ttgctcggtc
     3961 aacagcccat gcttcagggc gtagatcgca gcctgaactc ggtcatttat ctgtagcttc
     4021 tggaacactg tggagaggta gttcttgacc gttttctcgg caaggcagag ctcacgaccg
     4081 atctccttgt tactgagccc catggcgagc aacttcagga tctcgacctc cttgggagtc
     4141 agtcctgcaa caccctggtc gggctccacc tggctggcga gccggcgaaa ctccttcagt
     4201 actgttgtgg tcatcctggg atccaccaat gacgcgccac gagctaccat tctaatggcg
     4261 aagatcaggt cggcaggttt ggcatccttg agcagatagc ccctcgctcc tgccctcatg
     4321 gcatcgaaga cgtactgatc ctgccggtgc atggtcagaa cgattatccc tgtctccgct
     4381 cgctccgaca gaatctgctc ggtggcagct acccccccca ctaccggcat gtttatgtcc
     4441 atcagaatga catcgggcgc gagagtccgc gcctttgcca cagcctccat cccgtcggat
     4501 gcttctccaa ccacctcgat gtctcgctcc atttcgagca gttgccgaag cccttctctg
     4561 aaaagggtat ggtcatcggc aatcataacg cgtgctttct tcattactgc tactgcctac
     4621 ctccgtggag gattagtggg ggagtctatg gtgtcatccc aatggaaggt gttctacata
     4681 ataccataac ttggccgact tggacgaagg catctcgaga acttgccgct ctcaatacgt
     4741 tcggtgtacc atccgcctcg actcatacga cctaaaagga gtaacccgtg gccgcttcgc
     4801 agcactcctt gagcgaggaa gaagctttcg tctcccggga gatgatctct ctagcctcgg
     4861 agattctatc catcgacgag gcgtgccaca cggttctatc cctattcgca cgagtcttcc
     4921 catgcgtggg aagctggatg gccaccactc gaacgccaaa tggcgccctg caacttcgtg
     4981 cgacgagcgg cttctcctgg accggtgagg cagatctgtc ggctctatcc ctgcagcgcc
     5041 tggctgagat tcccagcgtg gagaccgtgc tatgggtctt cgacctcgag cactctccga
     5101 ctgtattgcc cttcgtggac gaagatgtag gcgccgttgt ggtcgcaaca ctgagcgcgc
     5161 acaacggctg tgtcggtatc gtggcgctga tgctccctac cccgtggtcc tttacgcccc
     5221 atcagcgaaa gctgctcgca tcactttcga atcaagctgc gctggtcgta catagcgcca
     5281 ttcttgttgc ggctgctcaa gagctcgcga tccaggaaga acgaagccgc atagccacag
     5341 agatccatga cggcctttcc cagaatctcg ccctcctcat gctcaaggtg gagattatct
     5401 cccgtcttat cgacagggac ccagagcggg caaaggcaga actggagaag atccagtcca
     5461 tagtcgaact tagcgttcag gagttgaggc attcaatcaa tacccttcgt tcaccggact
     5521 cggcagggct cggcctcatc ccagctcttc agcgcatggc cggagacttc accaagcgga
     5581 caggcataaa cgtcgaactc gcactaccct cggacctgtc gctaccacca caggccatat
     5641 cgacgatcct cggcgtggta caggaaagac tcggcacggc atccaggcag gcctccgcct
     5701 ccagagtcac cgtggctgtg gcaacggccg gcgactgcct caccgtgcgt atcgcggatg
     5761 acggccacgc ttccgttaac ccgccgttgg atgaagatgg tgcgacggtt ccattgctcg
     5821 ataggcttag agatcttgtt cgtcccctgg gcggcacagt ctcgaccacc gctgactcaa
     5881 cagggatggt ggtcgaaagc cggatccctt tgcgccagtg cacgacccca tcccactaag
     5941 cgactctccc ttgttggtat ccactccccg ctcatggaaa cgttgatggc gccgactccg
     6001 ttcccgctgt tggatacatt attccctagt agtttagtcg gtacccattg ccactccgac
     6061 atggtc
//
LOCUS       DATALS010000166         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_413469, whole genome shotgun sequence.
ACCESSION   DATALS010000166 DATALS010000000
VERSION     DATALS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413469"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..582)
                     /locus_tag="VHS06_03580"
     CDS             complement(277..582)
                     /locus_tag="VHS06_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987234.1"
                     /translation="MPRVLVIEDEKGARHVLGSALSRDGNEVQTVAQLDRAVDVARDF
                     QPEVIVADWLFPEGTVLAVAQELRSTFPSAVMIFMSGLPLNCFEGDAERFDPVAILQ"
     gene            758..994
                     /locus_tag="VHS06_03585"
     CDS             758..994
                     /locus_tag="VHS06_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987235.1"
                     /translation="MQKKLTNSQRLFYLGLLAMLLFATLSAAAWPLRPQAMALLTLAA
                     AVVTAGARLRYHLLHRAGLRDFLAALRRSELPQP"
     gene            1293..>1576
                     /locus_tag="VHS06_03590"
     CDS             1293..>1576
                     /locus_tag="VHS06_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEX2987236.1"
                     /translation="MASVCFYFQVHQPYRLRRYSVFESDHNYFDDYKNGEICRKVAHK
                     CYLPANRLMLEMIKKWEGRFRIAYSLSGVILDQFEQFTPEVMDTFQQLNET"
BASE COUNT          297 a          539 c          426 g          314 t
ORIGIN      
        1 gagagtgtcg cgaagaggag catggccagg aggcccaggt aaaacagccg ctgcgagttc
       61 gtgagcttct tctgcatggc caaacgtcct tcactggtcg tgagcgcaca tgcccgtaat
      121 cggggcgggt cggccgggcg attcagcccc cgacgaatac cgggcgcact tgttctatcg
      181 acagcaccgg acggacactg gagcggccgt tagggttgag cgtcagctca ggacggcccc
      241 tttgaccaga ttacgaatga gctcgagggt aaagggctat tgaaggatag cgactggatc
      301 aaaccgctcg gcatctccct cgaagcagtt gagaggcaat ccgctcataa agatcatgac
      361 cgccgaggga aaggtacttc gcaactcctg cgccacggcc agtaccgtgc cctcagggaa
      421 gagccagtcg gccacgatga cctccggctg aaagtccctg gccacatcta ctgcccggtc
      481 gagctgggcg accgtctgaa cttcgttgcc atctcgcgag agcgccgatc ctaagacgtg
      541 tcgggcccct ttctcatcct cgatgaccag tacccggggc atggatctac tccattctcg
      601 gctgctgagc cgctggtgtc ccggacagat ccggcccgct caatcaggca agataccgtc
      661 gatcccggca ttccattccg gcccacgcct cgctgcgcgg tttcctgttg cattgttccc
      721 ctcagatgtg ctcacgacca gtgaaggacg tttggccatg cagaagaagc tcacgaactc
      781 gcagcggctg ttttacctgg gcctcctggc catgctcctc ttcgcgacac tctcggccgc
      841 cgcctggccg ctcaggccgc aagccatggc cctcctcacc ctggccgcag ccgtcgtgac
      901 cgcgggggcg cgcctgcgtt accacctgct ccaccgcgcc ggactccgcg acttcctcgc
      961 tgcgctccgc cggagcgagt tgccgcagcc ctgatcaggc aacatcctga agcccgcact
     1021 ccctcgcacc caccttgtca gctccgacgt cccgacctcg ccacctccta cgttctcacc
     1081 ttcccacctt ctcaccttcc cacttgctca cgttcgcacg gtgtcacctt ctgtcacgct
     1141 ctcgcgtgcg aaaacccgtc gcacaaatcc gcctttgtac gcagaaatcg cactggctta
     1201 cggccgagct tcgggtacca tgctcatgtc tataatcgac gaaacctcag cttccgtatt
     1261 gccaaggcgc gcgggcgcaa ggggcgactt ctgtggcttc agtttgtttc tatttccagg
     1321 tccatcagcc gtaccgtctg cgtcgctaca gcgtattcga gagcgaccac aactacttcg
     1381 atgattacaa gaacggtgag atctgccgca aggtcgcgca caagtgctac ctccccgcca
     1441 accgcctcat gctggagatg atcaagaagt gggaggggcg ctttcgcatc gcctactccc
     1501 tcagcggcgt catcctggac cagttcgagc agttcacgcc ggaggtcatg gacaccttcc
     1561 agcagctcaa tgagac
//
LOCUS       DATALS010000167         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_150488, whole genome shotgun sequence.
ACCESSION   DATALS010000167 DATALS010000000
VERSION     DATALS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150488"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VHS06_03595"
     CDS             <1..214
                     /locus_tag="VHS06_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX2987237.1"
                     /translation="LRARLEAMGYVAPGSERAEEAGEEGEGAAVREPSATADEDDEDE
                     EDEPEGVANVDDDEPEEVEAGVDEDE"
     gene            238..591
                     /gene="rplQ"
                     /locus_tag="VHS06_03600"
     CDS             238..591
                     /gene="rplQ"
                     /locus_tag="VHS06_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX2987238.1"
                     /translation="MRHRVAGRKLGRPTDHRLALYRNLTTDLLRYEKIMTTEAKAKEV
                     RSMAEEMITKAKGGSLHARRQAMSDLYDPQVVEKLFATLAPRFQERQGGYTRIIHLGP
                     RKGDAAPMVQLELVI"
     gene            595..1347
                     /gene="truA"
                     /locus_tag="VHS06_03605"
     CDS             595..1347
                     /gene="truA"
                     /locus_tag="VHS06_03605"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX2987239.1"
                     /translation="MRNVKLVVEYDGGDYHGFEIQVGLRTIQGELERAIALVTETQTR
                     VVGSGRTDAGAHALGQVVSFRTGSRLSCWDLRRALNATLPQDIVVKSVEEVAESFHAR
                     RSAARRNYRYSIWNAQLRSVWTRRYRHQVIDPLSVEAMNLAAEVLVGTRDFACFTYGL
                     NRYLGEGKFRTTVRTLFTARWSGKGPELDFDVSGTAFLPHMIRNMVGSMLRVGVGKLT
                     PEEYRGLVESGAEQLSAVTVPACGLTLVSVEY"
     gene            1358..1792
                     /gene="rplM"
                     /locus_tag="VHS06_03610"
     CDS             1358..1792
                     /gene="rplM"
                     /locus_tag="VHS06_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX2987240.1"
                     /translation="MKTYSPKASEIVRQWHVIDASGQTLGRLATEIAQLLKGKNKVIY
                     APHMDTGDFVIVVNASKVRVTGDKLAEKKYYRHSGYPGGLTAVSLASQLEKFPERVVE
                     HAVRGMLPHNALGRAMYRKLKVYGGPTHPHASQLTKQEQPSA"
     gene            1927..2313
                     /gene="rpsI"
                     /locus_tag="VHS06_03615"
     CDS             1927..2313
                     /gene="rpsI"
                     /locus_tag="VHS06_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX2987241.1"
                     /translation="MDQIAAVGRRKTAVARVVLVPGSGAVMINGMELKDRFARAAWQD
                     MITLPMKLTNSLGNYTATIRVHGGGVVGQAGAIRHAIARALAKADEAMKPTLRKAGLL
                     TRDPRVKERKKYGLKRARKAPQYTKR"
     gene            2527..>2757
                     /locus_tag="VHS06_03620"
     CDS             2527..>2757
                     /locus_tag="VHS06_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987242.1"
                     /translation="MSDNTELLAKQGASAKQMSLSKALLVSVRPKQWSKNGLLFLGLV
                     FSRTLTHPDLLVRASLAFLDFCLLASATYLIND"
BASE COUNT          568 a          710 c          959 g          520 t
ORIGIN      
        1 actgcgtgcg cgcttggagg cgatgggcta cgtggctccc gggtcggaac gggccgagga
       61 ggccggcgag gaaggcgagg gcgctgcggt gcgcgagcct tctgccacgg ccgatgagga
      121 cgatgaggac gaggaagacg agccggaggg tgtggccaac gttgacgacg atgagcctga
      181 agaggttgag gccggcgtcg acgaggacga gtaggggtaa ctgggaggaa ctggcgagtg
      241 agacatcgtg ttgctggccg aaagctggga aggcccactg atcaccggct ggccttgtat
      301 agaaacctga caaccgacct gctgcgatat gagaagatca tgacgaccga ggcgaaggcc
      361 aaggaagtca ggagcatggc cgaggaaatg atcaccaagg ccaagggcgg aagcctccat
      421 gcgcgacggc aggcgatgtc ggatctgtac gatccgcagg tggtggagaa attgttcgcc
      481 actctggctc cacggttcca ggagcgacag ggtgggtata ccaggatcat tcatctgggt
      541 ccccgaaagg gtgatgctgc accgatggtg cagctggagc tggtgatcta gtagttgcgg
      601 aacgtcaagc tggtagtcga gtacgacggt ggtgactacc acgggttcga gatccaggtt
      661 ggacttcgga cgatccaggg agagctggaa cgggccattg ccttggtcac ggagacccag
      721 acccgagttg ttgggtccgg gcgaacggat gccggggctc acgctctcgg gcaggtagtc
      781 tcgtttcgga cgggctcccg gctttcctgt tgggatctca gaagggcgct caacgcgacc
      841 ttgcctcagg atatcgtggt gaagtccgtt gaggaggttg cggagagctt ccacgcgcgg
      901 cggagcgccg cgaggaggaa ctaccgctac tcgatctgga acgctcagct tcgctccgtt
      961 tggacgagac ggtaccgcca tcaggtgatc gatccgctga gtgtggaggc gatgaacctg
     1021 gcggcggagg tgctggtggg gacacgggat ttcgcctgct ttacctacgg gctgaaccgg
     1081 tatctcggcg agggtaagtt ccggacgacg gtgcgaacgt tgttcacggc gcgatggagc
     1141 ggcaaggggc cggagctgga tttcgatgtg tcaggcacgg cgttcctgcc gcacatgatc
     1201 aggaacatgg ttggctcgat gctcagggtt ggggtcggga aattgactcc agaggagtac
     1261 cgcggtctgg tagaatcggg ggcagaacag ctctcggcgg taacagtgcc ggcctgcggg
     1321 cttacgcttg tatcagtgga gtactgaagg agggcgggtg aagacttaca gtccaaaggc
     1381 ctccgagatt gtcaggcaat ggcacgttat cgatgcatcc ggccagacac tcggtcggct
     1441 tgctaccgag atagcgcaac tgctcaaggg caagaataag gttatctatg ctccccacat
     1501 ggacaccggg gattttgtca tcgtcgttaa tgcgtcgaag gttagggtga ccggtgacaa
     1561 gctggcggag aagaagtact atcggcactc agggtatcct ggagggctca ccgcggtgag
     1621 cttggcgtca cagctcgaga agttcccaga gagggttgtc gagcatgccg tgcgcgggat
     1681 gttgcctcac aatgcgttgg ggcgggcgat gtatcggaag ctgaaggttt acggtggacc
     1741 tacccaccct cacgcctccc agttgacaaa gcaggagcag ccctcggctt aagggggagc
     1801 tatcagcaat cagcaatcag cggtcagctt gcggtcaagt tgggcagatc ggccgttgtc
     1861 agtcggtagg gctggcggct gagccctgat ggctgacagc tactaaagga gttggtagtg
     1921 gcaaacatgg atcagatcgc agcggtggga cgacgcaaga ccgccgtggc acgggttgtt
     1981 ctggtgcctg gcagcggtgc ggttatgatc aacggcatgg agttgaagga ccggttcgcc
     2041 agggctgcct ggcaggacat gatcacgctg ccgatgaaac ttacaaatag tctcggcaac
     2101 tataccgcga ccatccgagt tcatggcggc ggcgtcgtcg ggcaggccgg agcgatccgg
     2161 catgcgatag ctagggcgtt ggccaaggcc gacgaggcga tgaagccgac attgaggaag
     2221 gccggtctct tgaccagaga ccccagggtc aaggagcgga agaagtacgg tctgaagcgg
     2281 gcccgcaagg cgcctcagta caccaagagg tagtctcggc tttcactgtg gatggcgaga
     2341 tcccgtccgg ccgatggcac agccccaacc catttgggtt ggggcttttt gttgcctggg
     2401 aatcctgggc atctcgtagg ggcaaccatg cgtcgcccct accggacgat aatggggcgg
     2461 ttagcggacg aagtagagaa tgagtgtgga gaccacaccc cagccgaggc cggcatagga
     2521 gcaacggtgt ccgacaacac ggagttactg gcaaagcagg gcgcctccgc gaagcagatg
     2581 tccctgagca aggcgcttct cgtttctgtt cgtccgaagc agtggtccaa gaacggtctc
     2641 ctcttcctcg ggctggtgtt ctcccgcact ctcactcacc cagacctctt ggtcagagca
     2701 tctttagcct tcctggactt ctgcctgttg gcttccgcca cctacctgat caacgac
//
LOCUS       DATALS010000168         4876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_373802, whole genome shotgun sequence.
ACCESSION   DATALS010000168 DATALS010000000
VERSION     DATALS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373802"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..595
                     /locus_tag="VHS06_03625"
     CDS             110..595
                     /locus_tag="VHS06_03625"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX2987243.1"
                     /translation="MPGVNEQRVVQQLTTRDVVSHWDVLELAFRATAATTARLLDPIL
                     ERMPFKVKAIQVDGGSEFMAEFEEACQARGIRLFVLPPRSPKLNGAVERANRTHTEEF
                     YHWSVADPTVAELGPELRAWETIYNTVRPHQALGYLTPKQFLDQWYQQHPREEVLSRT
                     L"
     gene            653..726
                     /locus_tag="VHS06_03630"
     tRNA            653..726
                     /locus_tag="VHS06_03630"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:685..687,aa:Gln,seq:ctg)
     gene            1121..1966
                     /locus_tag="VHS06_03635"
     CDS             1121..1966
                     /locus_tag="VHS06_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987244.1"
                     /translation="MKRLVLVAAVILAAMLIPGNIATAQSKPEFRLGFKALADQVPNV
                     VGLPIENEHWGANGDSLQQTTTGLMAWRKSDNWTAFTNGSRTWVNGPLGVMERGNDER
                     FVWESSDTEEDDDISESEDIPATSTSTSSSTASVLSLFGQPFSSDTVQGFINGNHCRL
                     RVPSVWSGGDATYQCMGKGISLGGSASTVLMIHLFAEGVDDYSAYPDTLPYGLRWGES
                     WSSVLGRLGTPAKQYEPGRYPPFRARADYNVDGARWSSVQLEFSADKGAERKLISVIF
                     VNSRC"
     gene            2188..2760
                     /locus_tag="VHS06_03640"
     CDS             2188..2760
                     /locus_tag="VHS06_03640"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX2987245.1"
                     /translation="MKATELRGMPLVSVKDAKRIGKVQDVIVDTTGQRVVALQAKTDS
                     EEPSFITLDRIHAVGKDVITLEEEKPAKERRGHPGAKGVGKEGVFPFKELHGNRVITY
                     DGTFLGTLSDLDIDTSDFRINEYEVSSSALSEITKSYKRIRATPDMHIGEGIITVSEQ
                     AYGEPSKQEVEHKKPEGVHEKEKEKRSHHR"
     gene            complement(2978..3184)
                     /locus_tag="VHS06_03645"
     CDS             complement(2978..3184)
                     /locus_tag="VHS06_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987246.1"
                     /translation="MGVDTVSLGQRVVWWPEPNTGWNSQQGVPAVVKKTVGDRMQIQA
                     RRWNGDPVLIWTTAERLRPESAKK"
     gene            complement(3611..4237)
                     /locus_tag="VHS06_03650"
     CDS             complement(3611..4237)
                     /locus_tag="VHS06_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987247.1"
                     /translation="MTHSLHRRGSRESLRGDYVMVCRTSKVNKAATGPALSRIAEIIF
                     EVGISNTGSSYLETNIPLGLDREMFKRRISEAHGLLCSFSSKEKLAQVLAKIHEEKLG
                     ISITVSGLIDEVIPLAQSMGIKPHTINLSMGILGKTGKLADEELLEYTTMCGHALIAA
                     NLVKKGIADVASGKKTAREASMMMGAPCICGIYNLDRSDLMLERSASK"
     gene            complement(4239..4691)
                     /locus_tag="VHS06_03655"
     CDS             complement(4239..4691)
                     /locus_tag="VHS06_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987248.1"
                     /translation="MKTNDVTGRVKRGEIGIGLEFGRPGVGTRLFELEKMTMALAGVG
                     VHFEPLNPVTFLLANPETGEMKPELRNERVLSAIVEIQTTPDKLSRLVPVIMDVADRV
                     DTVISWEIITRLDDDGAIPIMDELRRLNALPRENAKINLGMGRPLAED"
BASE COUNT          997 a         1542 c         1415 g          922 t
ORIGIN      
        1 ggtcacctgg cactataatg gctgttagaa gatcacagtt gaagcaatag cagcgctccc
       61 ggccggaccg ggacacgtaa ttcagtgttg gaggacggca gtggccgcaa tgccaggtgt
      121 caacgagcag cgtgtggtcc agcagttaac caccagagat gtcgtgagcc actgggacgt
      181 gctggaactg gcttttcgcg ccacggcagc caccactgca agactgctgg accccatcct
      241 ggagcgaatg ccgttcaagg taaaggccat ccaggtggat ggaggcagcg agttcatggc
      301 ggagttcgag gaggcgtgcc aggccagggg gatcaggctc ttcgttctcc ctcccaggag
      361 tcccaagctg aacggggccg tggagagggc caacaggacc cacaccgagg agttctatca
      421 ttggtctgtg gccgacccaa cggtggcaga actcgggccg gagctccgag cctgggagac
      481 gatctacaac accgtcagac cccaccaggc tctcggctac ctgacaccca agcagttcct
      541 tgaccagtgg taccaacaac acccgagaga ggaggtcttg tcacggacgt tgtgaaccca
      601 tacagggatt tgcctaccgg cgggcggccg tgctataatc tcctttgtcc gttgggggat
      661 cgtctagtgg taggacagct gactctggat cagtaagccg gggttcgaat ccctgtcccc
      721 cagccaacga aacggcctgg attgcacgcc aggccgtttt ttcgtgcctg gatagagccc
      781 ttccagccta tttttgccac ctcagagcct ccaaacagag ccgtctccga gaccgccccg
      841 gcgccccgct gacgttgtac ggatgttgta agctcccatc tgtctgctcg gcgtgccggc
      901 tccgctccgg ggccaaccat ctctacacct tcatcgcagc accatgtgtc ggactttgca
      961 agtacggccg ccctcgcgtg catacatcca gccccaagat gtcttgcccg ccggcagcgg
     1021 gagtgctacc atccgcaaca acttggttgc tcgctccatc acatccccta cattcacagt
     1081 acatcggcga gtcagatccc agcgaaaatg gagagtgtgg atgaagcgtc tcgtgctggt
     1141 cgcagcagta atcctggccg ccatgcttat ccccggcaac attgccactg ctcagtccaa
     1201 acccgagttc aggcttgggt tcaaggccct ggccgaccag gtccccaatg ttgtaggtct
     1261 cccgatcgaa aacgagcact ggggggcaaa cggcgactca ctgcagcaaa cgacgactgg
     1321 actaatggcg tggcgcaaga gcgacaactg gaccgccttc accaacggca gccgcacctg
     1381 ggtcaatggt ccactgggtg tcatggagag aggcaacgac gagcggttcg tatgggagag
     1441 tagcgatacc gaggaggacg acgatatttc ggaaagcgag gatattccgg caacctcaac
     1501 ctccacctcg tcaagtaccg cctcggtgct cagcctgttc gggcagccgt tctcttcgga
     1561 cactgtccag gggttcatca acggcaacca ctgcaggctc cgtgtccctt cggtgtggtc
     1621 gggtggcgac gcaacctatc aatgcatggg aaaaggcatc agtctcggtg gcagtgcgag
     1681 cacggtgcta atgatccacc tcttcgccga gggggtcgat gactatagtg cctaccccga
     1741 cactctgccg tatggcctgc gttgggggga gtcatggagc agtgtcctcg gacgcttggg
     1801 gactccggca aagcaatacg agccgggtcg atacccaccc ttcagggcca gggccgacta
     1861 caacgtcgat ggcgcacgct ggtcgtcggt gcagcttgag ttcagcgcgg acaagggggc
     1921 cgaacgtaag ctgatctcgg tgatctttgt gaacagccgg tgctagcctc agtcacagga
     1981 cggccaaccc tggatcagta agtggggtcg gatgcttgct cccgaccaac gagacggcct
     2041 gggctgccct ccaggccgtc tttcccattt gggacggtct acccaatgga aggcgatgcg
     2101 tcgccgcctc cttctacggg cacacaatct gcccccttcg gccgtggcac cgcgtggtgc
     2161 cgcatcacca gagaaggggg cagcgacatg aaggctaccg agcttcgagg aatgccgctg
     2221 gtatcggtga aggatgccaa gagaatcggc aaggtccagg acgtcattgt ggacaccaca
     2281 ggacagcgag tagtcgccct ccaggccaag accgactccg aggaacccag cttcatcacc
     2341 ttggatagga tccatgccgt cgggaaagat gtcatcaccc tcgaagaaga gaaaccggcg
     2401 aaggagagac gtggccaccc cggggccaag ggggtcggca aagagggtgt attccccttc
     2461 aaggaacttc acggcaacag ggtgattacc tacgacggaa cgttcctcgg caccctgagc
     2521 gatctcgaca tcgatacctc cgacttcagg atcaacgagt acgaggtgag ttccagcgcg
     2581 ctctctgaga tcacaaagag ctacaagcga atccgcgcga ctccggacat gcacatcggc
     2641 gaggggatca tcaccgtgtc cgagcaggcc tatggtgagc cgagcaagca ggaagttgag
     2701 cataagaagc cagagggagt ccacgagaag gagaaggaga agcgcagcca tcaccgctga
     2761 ccggcgacat cctctatagt gagtcggaag cagtgtcaag ctgcggcaag cgtgggcggc
     2821 ggtgaccggt ggcacgagag gccggctacg aatgccgctc gccgccagtc tcttgcgtcg
     2881 gtcacgttgc gagtttaggc gcgcgggcag gcgacggctt tcacacgccc tcggtcgcct
     2941 cgccgcgaga ccatcatgaa gagactcagc gccgagccta cttcttcgca ctctcgggcc
     3001 gcagtcgttc ggccgtggtc cagatcaaca ccggatcacc gttccatcga cgagcctgaa
     3061 tctgcattcg atcaccaacc gtcttcttga cgaccgccgg caccccttgc tgcgagttcc
     3121 acccggtgtt tggctcgggc caccacacta cgcgctgtcc cagagaaacg gtgtccactc
     3181 ccatggctcg tccctcatcc actgcgatcg acgcctcact ctagttccag caattggcga
     3241 gacacgaccg gcccatcaag ggcactgcga ccgggaagaa acaacgatga ctccgcgcta
     3301 agtgtacgag gtaatgataa caacttcctg acagtagatg catatttcgc gccaagaaac
     3361 ggccaaatcc gaaatccaat cccggctccc tgccttgtgg atgacagatc tgtcagacga
     3421 gagggtattc tgaacaaaag gagagggcag ggtgtcagac cctgccctcg cgcggccctc
     3481 gacccagcgc tgcccccagc gccaagtcga agcgtcccta cccattcaac agggcatgcc
     3541 cggatccacc acttgtgcgc gccgcacgtc gaccgcatct gatcacggac agtacggatg
     3601 cgccccgaag ctactttgag gcggacctct ccagcatcag atcgctgcgg tcaaggttgt
     3661 agattccaca aatgcagggt gcccccatca tcatggatgc ttccctggcg gtcttcttcc
     3721 ccgacgctac gtcggcaatc cccttcttga cgagattggc cgcgatcagc gcgtggccgc
     3781 acatcgtcgt gtactccagc aactcctcgt cggccaactt cccggtcttc cccagtatcc
     3841 ccatggacag gttgatggtg tgcggcttga tccccatcga ctgggccaac gggatcacct
     3901 cgtcgatcaa cccgctcacg gtgatggaga tccccagctt ctcctcgtgg atcttcgcca
     3961 gcacctgcgc cagcttctcc ttgctggaga agctgcacag aagcccgtgc gcctcggaga
     4021 tcctgcgttt gaacatctcc cggtccagcc ccagggggat gttggtctcg aggtaggagg
     4081 aaccggtgtt ggagatgccg acctcgaaga tgatttcggc gatcctcgac aacgcgggac
     4141 cggtcgcggc cttgttcacc tttgacgtcc ggcaaaccat aacgtagtcg cctcgaagcg
     4201 actcccgcga gcctcttctg tgcaacgagt gcgtcatcct aatcctccgc caaaggacgg
     4261 cccatgccga gattgatctt cgcgttctcc ctgggcagcg cgttcagccg cctcagttcg
     4321 tccatgatcg gaattgcccc gtcgtcgtcc agccgagtga tgatttccca ggagatcacc
     4381 gtgtccactc tgtccgctac gtccattatc accggcacca gccgcgacag cttgtccggc
     4441 gtggtctgga tctctacgat ggccgacagc acgcgttcgt tcctcagctc gggcttcatc
     4501 tcccccgtct cagggttcgc cagcaggaag gtcacggggt tcagcggctc gaagtggacg
     4561 cctacgccgg ccagcgccat ggtcatcttc tcgagttcga agagtcgcgt cccaacaccg
     4621 ggtctcccga actccagacc gatgccgatc tcacccctct tcaccctacc ggtcacgtcg
     4681 ttggtcttca tctcctccgt gccacggccc cgcccgccgg tgctgctcga gtgcggcgtc
     4741 atgggatcgc tgaactcacg ccggagcttt cttggccact gggaggtttc cggggactcg
     4801 cagattgctg ccgatggaca cggtgcgaac cggaggcagg tcgtgcactc gacgcaacca
     4861 tcgaaatcga ttgtcg
//
LOCUS       DATALS010000169         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_97982,
            whole genome shotgun sequence.
ACCESSION   DATALS010000169 DATALS010000000
VERSION     DATALS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97982"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1885
                     /locus_tag="VHS06_03660"
     CDS             <1..>1885
                     /locus_tag="VHS06_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2987249.1"
                     /translation="DLTEAARKGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGV
                     GKTAIAEGLAQRIARGDVPEGLKNRRLVALDLGSLIAGAKYRGEFEERLKAVLKEVQD
                     SEGEVILFIDELHTVVGAGAAEGAMDASNMLKPMLARGELHCVGATTLDEYRKHIEKD
                     AALERRFQPVPVPEPSVEDTISILRGLKERYELHHGVRIQDPALVAAAVLSQRYITDR
                     FLPDKAIDLVDESASRLRMEIDSMPAELDEIERRIMQIQIEREALRKETDPTSQQRLA
                     DLERELSGLRERSDEMKAQWQREKETIQRHQKLKGEIEQVRLEGEKAERESDYAKAAE
                     IKYGRLNELERQLKEEESKLASLQSQKRLLKEEVDEEDIAEVVSKWTGIPVSRLVEGE
                     IQKLLRMEERLHLRVVGQDEAIQAVSNAVRRARAGLQDPNRPLGSFIFLGPTGVGKTE
                     LARALAQFLFDDEQAMVRIDMSEYMEKHTVSRLIGAPPGYVGYEEGGQLTEAVHRRPY
                     SVVLFDEIEKAHPEVFNVMLQILDDGRLTDGHGRTIDFKNTVLIMTSNVGSELYQLGQ
                     KSGVSMDHVKEQVNEALKAHFRPEFLNRIDEIIVFHPLEMAHLKQIVDIQLARLESRL
                     ADK"
BASE COUNT          407 a          543 c          666 g          269 t
ORIGIN      
        1 gcgacctcac ggaggccgcg cgcaagggga agctggaccc ggtgatcggc cgcgacgagg
       61 agatccgtcg cgtgatccag gtgctctcca ggcgcaccaa gaacaacccc gtgctgatcg
      121 gcgagcccgg cgtgggcaag accgccattg cagaggggct ggcacagcgg atcgctcggg
      181 gcgacgtccc ggaggggctg aagaaccgcc ggctggtggc gctggacctg ggctccctga
      241 tcgcgggcgc gaagtaccgc ggtgagttcg aagagcggct gaaggccgtg ctgaaggagg
      301 tgcaggactc cgagggagag gtgatcctct tcatcgacga gttgcacacc gtcgtgggcg
      361 ccggcgctgc cgagggcgcc atggacgcat ccaatatgct gaagccgatg ctggcccggg
      421 gagagttgca ctgcgtggga gcgacgaccc tggacgagta tcgcaagcac atagagaagg
      481 acgccgcgct ggagcgccgt ttccagcctg tacctgtgcc ggagcccagc gtggaggaca
      541 ccatcagcat cctgcgaggg ctgaaggagc ggtacgagct gcaccacggc gtgcggatcc
      601 aggaccccgc gctggtggct gccgcggtcc tctcccagcg ctacatcacc gaccgcttcc
      661 tgcccgacaa ggcgatagac ctggtggacg aatcggcctc ccggctgcgg atggagatcg
      721 acagcatgcc cgccgagctt gacgagatcg agcggcggat catgcagatc cagatcgagc
      781 gggaggcgct acgcaaggag accgatccca cttctcagca gcggctggcg gatctcgagc
      841 gggagctatc agggctgagg gaacgctccg acgagatgaa ggcgcagtgg cagcgggaga
      901 aggagacgat ccagcgccac cagaagctga agggagagat cgagcaggtc cgactggagg
      961 gcgagaaggc ggagcgcgag tccgactacg ccaaggccgc cgagatcaag tatggccggc
     1021 tcaacgaact ggaaaggcag ctaaaggaag aggagtcgaa gctggcctcg ctacaatccc
     1081 agaagcggct gctcaaggaa gaggtcgacg aggaggacat cgcggaggtt gtctccaagt
     1141 ggaccgggat cccggtaagc cggctggtgg agggggaaat ccagaagctg ctgcgcatgg
     1201 aggaacggct ccacctgagg gtcgtaggcc aggacgaggc gatacaggcg gtttccaacg
     1261 cagtgagaag ggcgagggca gggcttcagg accccaaccg cccgctgggc agcttcatat
     1321 tcctgggtcc caccggcgtc ggcaagaccg agctagccag ggcactggcg cagttcctct
     1381 tcgacgacga gcaggcgatg gtgcgcatcg acatgagcga gtacatggag aagcacaccg
     1441 tctctcggct gatcggtgct cctccgggct acgtgggtta cgaggaaggc ggccagctca
     1501 ccgaggcggt ccaccgtcgg ccatacagcg tggtgctgtt cgacgagatc gagaaagcac
     1561 accctgaggt gttcaacgtc atgctccaga tcctggacga cggtcggttg accgacggcc
     1621 acggcaggac catcgacttc aagaacaccg tgttgataat gacctccaac gtgggcagcg
     1681 agctgtacca actgggacag aagtccggcg tctccatgga ccacgtaaag gagcaggtga
     1741 acgaggcgct gaaggcccac ttcaggccgg agttcctgaa ccggatcgac gagatcatcg
     1801 tattccaccc gctggagatg gcccacctga agcagatcgt ggacatccag ctggcacggt
     1861 tggagtcgcg attggctgat aagaa
//
LOCUS       DATALS010000170         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_150855, whole genome shotgun sequence.
ACCESSION   DATALS010000170 DATALS010000000
VERSION     DATALS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150855"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VHS06_03665"
     CDS             <1..1060
                     /locus_tag="VHS06_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEX2987250.1"
                     /translation="GARVVVPTSLNAGPIDEEGWRNIPVTEEFAEKSLRIMRAYVKMG
                     CTPTWTCAPYQSGVRPAKGEQVGWSESNAIVFANSVLGARTSRYGDYVDLCMALTGRA
                     PKSGLHLTENRRGQILFRLAGLPAALLQSDSFYPVLGFYLGKMAGQKIPVLDGLPSGV
                     TEDQLKALGAAAASSGCVALFHAVGITPEAPTLEAAFQSQQPEEVVEVKLSTLKECRR
                     QMSTCSQGALDVVILGCPHFSQDEFRKLGRLVKGRNRHPGTRLVVMTSRSVRDAVKAA
                     PFWPELERFGVEIVADACVLLTPMLRSEDRFVMTGSGKCAYYGPGVLRRKVAFGSLED
                     CVESAVLGQVCREESLWN"
     gene            1051..1482
                     /locus_tag="VHS06_03670"
     CDS             1051..1482
                     /locus_tag="VHS06_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEX2987251.1"
                     /translation="MELMTGTPLVEGDASGSALVTSEPLSLWGGLDPKSGEVIDRRHE
                     LSGQKITGRVLVLPYSRGSTTASTVLLEAIRAGTAPAAIVASCADPMLALGAVVAGEL
                     YNRVVPVVALNETDFQRLRTGDRVAIQSDGTVELTAEATTA"
     gene            1554..2333
                     /locus_tag="VHS06_03675"
     CDS             1554..2333
                     /locus_tag="VHS06_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX2987252.1"
                     /translation="MAAENAPRAAIESVRSVERTLDLLAALERAEQPMGLSDLARATG
                     IPKATAQRLLTVLERRGFVQKERLSYQLGAAVVPMAGAFLTGSSLARAALPALEELAM
                     ASSETASLQVRQGVDRVVVQRVLSVHSLGYSLHIGQRLPLLVGASGLILAGGMPSEGL
                     SLLMDSVGEIRLADGRLLSHTDLLAKIEAVRRQGFAVSRGEREGGVISVAAPILRTGY
                     PLNAAIAVTGPTSRMAEEKVDQLSITVKQVAADIAKVYSRM"
BASE COUNT          475 a          718 c          785 g          459 t
ORIGIN      
        1 aggcgctcgt gtggtagtgc caacctcact gaacgccggt cccatagacg aggaggggtg
       61 gcgcaatatt cccgtgacgg aagagttcgc ggaaaaaagt ctgaggatca tgcgtgcata
      121 cgtcaagatg ggttgcacgc caacctggac ctgtgcccca tatcagtcgg gagttcgacc
      181 ggcaaagggc gagcaggttg gatggtcaga atccaatgcc atagtcttcg cgaactcagt
      241 gctgggagca cgaaccagcc gatacggaga ctacgtggat ctgtgtatgg cactcacggg
      301 gagggctccc aagagtgggt tgcacctcac cgagaatcgg cgaggccaaa tcctattcag
      361 gcttgccggt ctgcctgctg ccctcttgca gagcgacagc ttctacccag tgctcggctt
      421 ctacctgggc aagatggcag ggcagaagat acccgttttg gatggattgc cttcgggagt
      481 gaccgaggac caactgaaag cgcttggagc ggccgctgcg tcctcgggtt gtgtagcgct
      541 cttccatgca gttggaatca caccggaagc gcccaccctc gaggctgcct tccagtcaca
      601 gcagcctgaa gaggtggtgg aggtgaagct gtccacgctc aaggagtgcc gtaggcagat
      661 gtccacctgc tcacagggcg ccctcgacgt cgtgatactt ggatgccccc atttctcgca
      721 agacgagttc agaaagctgg gtcgattggt gaaaggtagg aatcgtcatc ccgggacccg
      781 cctggtagtg atgaccagcc gatcggtgcg agatgcggtg aaggcagccc cattctggcc
      841 ggagctggag cggttcgggg tggagatcgt ggcagatgct tgtgtcctcc tcacgccgat
      901 gctccgtagc gaagatcgct tcgtaatgac cggttctggg aaatgcgcct actacgggcc
      961 gggcgtcctt cgaaggaagg tagccttcgg gagcctggag gactgcgtcg aatctgccgt
     1021 tttgggacaa gtttgcaggg aggaatcgct atggaactga tgacaggtac cccgctcgta
     1081 gaaggagatg cgtcgggatc ggccctggtg acgagtgaac ccctcagtct gtggggtggc
     1141 ctggatccga agtccgggga agtaatagat cggcgtcacg aattgtcggg gcaaaagatc
     1201 acagggcgag tgctggtgct gccctactcg cgaggctcca cgacagcaag caccgtgctg
     1261 ctggaggcca tccgggcagg aacggcaccc gctgccatcg tcgcttcttg cgcagatccg
     1321 atgctggcgc tgggagcagt agtagcaggc gagctgtaca accgggttgt gccggtagtt
     1381 gccctgaacg agacagactt ccagcggctt cggacggggg atcgagtggc catccagtcc
     1441 gacgggaccg tcgaactgac agccgaggca acaacagctt gacagttgtt ccagccagtg
     1501 gtactctcgt catcggtccc attgtgcagt atgttgttcc atcaggtggt acaatggccg
     1561 ccgagaacgc cccccgcgcc gcgatagaat cggtccgttc cgtcgaacga accctggatc
     1621 tgctcgccgc actcgaacgc gccgagcagc ccatgggttt gagcgacctc gctcgcgcga
     1681 ccggcatccc caaggccacc gcccagcggc tcctcacggt gctggaacgc cgcggcttcg
     1741 tccagaagga gcggctcagc tatcaactgg gcgccgcggt cgtgccgatg gccggcgcct
     1801 tcctcaccgg tagctccctt gcccgcgccg cgctccccgc cctggaggaa ctggccatgg
     1861 cctccagtga aaccgcttct ctccaggtgc gacagggggt cgaccgagtc gtcgtccaac
     1921 gcgtactcag cgttcactcc ctcggctaca gcctgcacat cgggcagaga ctgccgcttc
     1981 tggtgggagc ctccgggctg atcctggccg gcggtatgcc ctcggaggga ctgtcgctac
     2041 taatggattc cgtcggcgag atccgactcg ccgatggcag actcctctcc cataccgatc
     2101 ttctggcaaa gatcgaggcc gtgcggcgcc agggtttcgc tgtaagccgg ggtgaacggg
     2161 agggtggggt aatatcggtg gccgctccca tactgcgaac cggctatcct ctcaacgccg
     2221 ctatagctgt gaccggcccc acctcccgga tggccgagga gaaggtcgat cagctctcca
     2281 taaccgtcaa gcaggtggca gccgacatag ccaaggtgta cagccgtatg taggccgagt
     2341 ggcacaaccg aattactgtt ctccatttct cagcgccggc agcaaggtgg caggccggcc
     2401 gacaggagga actgctgatg aaggaagcat cgtctgg
//
LOCUS       DATALS010000171         8574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_197260, whole genome shotgun sequence.
ACCESSION   DATALS010000171 DATALS010000000
VERSION     DATALS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197260"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(502..584)
                     /locus_tag="VHS06_03680"
     tRNA            complement(502..584)
                     /locus_tag="VHS06_03680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(549..551),aa:Leu,seq:taa)
     gene            765..1337
                     /locus_tag="VHS06_03685"
     CDS             765..1337
                     /locus_tag="VHS06_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX2987253.1"
                     /translation="MQYGIISDVHANLAAFEAVLQDMGPVDALWCLGDLVGYGPDPNE
                     CIELLRRHNHLCIVGNHDMAAVGRMDTEEFNPQAAFAADWTAATLTERSRNFLLGLPE
                     RLEAEPFTLVHGSPRSPIWEYITNARIAAANFEQFDTAACLVGHSHVPSLYIDEDGHV
                     IGQMPGAGDRVEIGPKPVIANPGGVGHLCC"
     gene            complement(1566..2348)
                     /locus_tag="VHS06_03690"
     CDS             complement(1566..2348)
                     /locus_tag="VHS06_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987254.1"
                     /translation="MNIRLFVAEWLRLLLKAKSIWGGIARTIVVLPGVAVFPLTLLRS
                     AGFEVPEPQAIIPGFSPDKPVHIPLVAMVTFYLAAASATGGAAWVLSRASSHIRMGEE
                     LEPDLENFMFRLRVWNDGDRNGFATVKVTQILDDSGQSMERVPLPLELEWSHHREMSH
                     IEVPRGPHGETVGVVGVTLGDAPPVLRAWGARHKGWVFGFNNPVDRGKVYLELVLDPE
                     GGKQVSRWFSLTPDSKSPLGYTARAEEPPNACSAAEGTKEYV"
     gene            complement(2387..3109)
                     /locus_tag="VHS06_03695"
     CDS             complement(2387..3109)
                     /locus_tag="VHS06_03695"
                     /inference="COORDINATES: protein motif:HMM:NF020757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HEX2987255.1"
                     /translation="MAHTLPAPWSIPLWATTNRKLLLSRPDWPQWKRIVDAILALLRS
                     RAAYVPALHSSRAKASPANSPRFSTQRGSGLMKYDDFSHRHGKELLQVLHPDTARQVC
                     EILSGLPAYSHGAKKGKTPKQYIAEAFVAQGWQQEGRADFRTTKRDYVDLCKWKVALE
                     MEYSRFEMFFRDFFRFMLLYERREIDVGIIITFSEGAYDRWEGETKAYRSARASLGKL
                     QDFLRGDYATIVDVPLWCIGIE"
     gene            3185..4033
                     /locus_tag="VHS06_03700"
     CDS             3185..4033
                     /locus_tag="VHS06_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987256.1"
                     /translation="MSEPYESRKKRMARGPVQEPHGGGPNRRKSRVAAGERTDRPYQL
                     EMEGIPERYDTAGDNEPGDESITGTRRWQAVSLAVKAWWRRQGDYARLGLVCAAGLLI
                     FWVFPSASGDYGWASHESRASDRTKLDVLASDNGERHPLHPWLWAWPAVTELWLARPE
                     EVQRAPASSGQRWVVANVQNKYHEGLAETTEDSFQIVYDCQRGLAAIGRYYAFSEAEA
                     VLYSSGLDGLRGGSPSWTPYREPHALGNPTRNFKAAQRGTLLEKAGGFVCRKVGYSWA
                     VESPQK"
     gene            complement(4336..5673)
                     /locus_tag="VHS06_03705"
     CDS             complement(4336..5673)
                     /locus_tag="VHS06_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2987257.1"
                     /translation="MADSSEAVALLPFLERRCRCGAAILETHSDRYICARGHTITKAE
                     AAKLWGVPASDSQPQGVNPDGGTSAPGGEGPSPKGNSNGTYRDDAVAVEPVYFKGLPR
                     PKPRCWVVAQFVPEGLPAMLYGAGGSTKSMVALTMGVCVAEGMPWLGREVSKRPVAYL
                     DFEFDQQEQHIRALAVTKGLDLEDVPEDFAYFPCVGHTMEQVQSKVEEWLDSNPGGLL
                     IIDSYGMASTGDAKDEEPVIAFMRGLRSIGAGGLLIIDHEAKVQVGQDRKHKTAYGSV
                     YKENLCRMVWHAVLEGSKDGKAMVTLLHHKTNMGPKSEPIQLEVVFLEDEDGSLDMVQ
                     MVEANPAAWPIEESANLQKRVADALRKQGPTTVTAIAATLNKNRGSVKTEVNRLQRQG
                     VVQSIGKQGREFIYAPVGNSNQDNPMGTVTVTDSSEPEGLYYDDEVEEEQQQD"
     gene            complement(5673..6089)
                     /locus_tag="VHS06_03710"
     CDS             complement(5673..6089)
                     /locus_tag="VHS06_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987258.1"
                     /translation="MEQVRETASNRPWGRSAVLAADTTNDGRSAHSNTRRQFYNLDGR
                     SVGSVTADGHLLKRVSTTRHMLRKPPAWAVDANHLEQLAAMNGRGVRLVDERGQVWEA
                     SLADFDRRGFDVNRGFGQQRGLLLRDWKVTPAGEAA"
     gene            complement(6077..6265)
                     /locus_tag="VHS06_03715"
     CDS             complement(6077..6265)
                     /locus_tag="VHS06_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987259.1"
                     /translation="MDMEEYRIKRLGFVLNPREYAALCRLSAQAGVSLAAVLRHLVRA
                     EAKRRAVWPADEVQPWSR"
     gene            complement(6380..7129)
                     /locus_tag="VHS06_03720"
     CDS             complement(6380..7129)
                     /locus_tag="VHS06_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987260.1"
                     /translation="MRLPRGFGIIDQRLPFVTLRVNADAHVVVAWFGYHTRGMEGSTF
                     QVPGGKVTLSGLVTYHTPSSYRLSICGLYLPDAPSTDETEAERYARELREQHPRLNEL
                     PDVIEIEVLSLGRGRSKGLGYCRNRALLDLFSGLLGEMERDFPETAEGVSAYRTAAAI
                     GDSNQSTGGGANTPDAPIPGYEVGVPARGPDYLRWHRIWQLVGKQAMDGKKIAAIADW
                     LGRLHPKEKVSTKTLGYIIRAGKEGRLDRPV"
     gene            complement(7308..8357)
                     /locus_tag="VHS06_03725"
     CDS             complement(7308..8357)
                     /locus_tag="VHS06_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2987261.1"
                     /translation="MSRKQERTYGVGSVYQRKSDGRWAAKLGNDGKPITRYAATEKEA
                     RAILAALLRECGQTGTLATPGNRTVNDLLDLWLQTARPSLKPRTFHDYQARCKRHLRP
                     VLGHVRLGKLSPEKVQAFYSKLLSCGTEREAQYGHAMLHRACKMAVLWGWLAQNPCDR
                     VIRPKHQASRKEMWSVDELQQFLEGTRGRWLHPLWVTAASTGCRLSELQGLRWQDVDL
                     AAAALRVCRSLHRIAGEWVESTPKTRAGDRTMVLPAAAVAALKGQRAQQAEWRLKAGP
                     QWQDTGLVFTGEYGQPLAQSTVQKAMHREVESWACRRSRPTDCATWRLPYSLARASPF
                     RQSRPAWATPAPISL"
BASE COUNT         1577 a         2682 c         2621 g         1694 t
ORIGIN      
        1 aggagaccaa accaccaact tccaaccagg atgagcaggg caactccgat tgcggcgatc
       61 cgagagattg ggctgtcctt ccggacctgt tgcggctcac agtcttcctg catgttggaa
      121 gtcctctcgc aaggcggatg gaccagaggg agcctctccc cggtcgtcag cagtctacca
      181 cgttgatcaa gatgattatc atcgccgggg gaagaccgct tcgaccttga cagccgatgc
      241 tacaatccgg gcatcaacaa cataagtgcc ccggcggtgc tggaacaccc cgaggcgtgg
      301 ccgggccagg ataagtggcc gggcaggctg agtgtagcac actgtggccc gccgggttat
      361 tcctggcggg ccttccgctt cgggggaagg atgggttgac acctatctga cacctattcg
      421 gtgtcaagcg gtgtcgggcc gggaggccga aaactgggca caaagaaggg gcgagaccca
      481 cgaaatgggc ctgccccacg gtaccgggga ggggactcga acccctactc ctttgcggga
      541 agcggatttt aagtccgccg cgtctgccgt ttcgccaccc cggcaaggtg cttcgccaca
      601 ctctccatct taccacacga gaagggtcac ggaaccacat cagccaggaa gccagccgcg
      661 gcgatcacgt ccgctcgagg ctggcaccgg ccgtgcggtg tccggctcgc gttgaagagc
      721 caggaccgct gtgctatagt cggtccccgc tggagggtag gagagtgcag tacggtatca
      781 tctccgacgt acacgccaat cttgccgcct tcgaggcggt gctgcaggac atgggaccgg
      841 tggatgccct ctggtgcctc ggcgacctgg taggctacgg ccccgacccc aacgagtgca
      901 tcgagctgct ccgtcgacac aaccacctgt gcattgtcgg caaccatgat atggccgcgg
      961 tgggccgaat ggataccgag gagttcaacc ctcaggcggc cttcgcggcc gattggacgg
     1021 cagcgaccct caccgagcga tcccgcaact tcctgctggg ccttcccgag cgactggagg
     1081 ccgaaccctt caccctcgta cacggtagcc cgcgctcgcc tatctgggag tacatcacca
     1141 atgctaggat cgccgctgcc aacttcgagc agttcgacac cgcggcctgc ctggtcggac
     1201 actcccacgt tccttccctc tacatcgacg aagacggcca cgtgatcggc cagatgcctg
     1261 gggccgggga cagggtggag atcgggccga agccggtcat cgccaacccg ggcggcgtcg
     1321 ggcacttatg ttgttgatgc cctcatgcta tcacggagtg tcaacatgac tcggtgacga
     1381 gcgctggccc cctatccggg tgcggggtct gcccatgcag gggtacgtcg atgacgactc
     1441 agcaccagag tcctcgtttg gtgagggccg catacgcctt ttcccaaaag aaggggaggt
     1501 gtatgcgcgt ggttaggcca aggcatgggt ggtggtgagg gcgggcgcct aatcaacagc
     1561 gcgttctaga cgtactcctt tgttccttca gcagcgctac aggcgttcgg cggctcctca
     1621 gcgcgcgcgg tatagcctag cggagacttg gagtcagggg tgagtgagaa ccagcggctg
     1681 acctgcttac ccccttcggg gtccaacacg agttcaaggt agaccttccc cctgtccacc
     1741 gggttgttga agccaaacac ccaccctttg tgccgagccc cccaggcacg gaggaccggt
     1801 ggggcgtctc ccaaggtgac cccgacaacg ccaaccgttt caccatgggg gccgcgaggc
     1861 acttcgatgt gggacatctc tcgatggtgc gaccactcca gctccagggg cagtggaacc
     1921 cgctccatcg attggccact gtcatctaga atctgagtga ccttgacggt ggcgaagcca
     1981 ttcctgtcgc cgtcgttcca gacccgaagc ctgaacatga agttttccag gtcaggctca
     2041 agctcttcgc ccatacggat gtgtgatgag gcacgagata gaacccatgc agcaccgccg
     2101 gtggcagagg ccgcagccag atagaaggtg accatggcca ccaggggtat atggacaggc
     2161 ttgtcagggc taaagccggg aatgatcgct tgtggttcgg ggacttcgaa gccagctgag
     2221 cgtagcagtg tcagaggaaa gacagcgaca ccgggcagca ctacgatggt tcttgcgatg
     2281 ccgccccaga tggatttcgc cttgagaagc agccgaagcc attccgctac gaacaacctg
     2341 atgttcatcg ccgtacctcc tcctggcacc aaagcacccg tctgtgtcat tcaataccga
     2401 tgcaccacag cggcacatca acgatggtgg cgtaatctcc tcgcaggaag tcctgcagct
     2461 tccccaggct cgccctggcg gacctatagg cctttgtctc tccctcccac cggtcgtagg
     2521 caccctcgct gaaggtgatg atgatgccga cgtctatctc cctgcgctcg tagagcagca
     2581 tgaagcggaa gaagtcccgg aagaacatct cgaaccggga gtactccatc tcaagagcga
     2641 ccttccattt gcacaagtcg acataatccc gtttggtggt gcggaagtcg gctcggcctt
     2701 cctgctgcca gccttgagcc acgaacgcct cagctatgta ctgcttcggt gtcttgccct
     2761 tcttggcacc gtgggagtaa gcgggcaacc cagagagtat ctcgcacacc tgccgggcgg
     2821 tatccggatg gagcacctgg agcagttcct taccatggcg atggctgaag tcgtcgtact
     2881 tcatcagtcc gctgcctcgt tgggtggaga atctgggcga gttggcgggg ctggccttgg
     2941 cccttgagga atgcagcgcc ggaacgtagg ccgcgcggga cctgagcaac gccagtatag
     3001 catcaacaat gcgcttccac tggggccagt ccggcctgga gaggagcagc tttcggttgg
     3061 ttgtcgccca caggggaatg ctccagggag cggggagcgt atgcgccaca aagaagggga
     3121 gcttgaagtc gagcccgacc tggtagatga tgccgccgat tgtctttggg tgaggaggta
     3181 gtgagtgagc gaaccttacg agtcacgcaa gaagcggatg gcgagggggc ctgtacaaga
     3241 accccatggt ggcggtccta acaggcggaa gagccgcgtt gctgccggag aacgtactga
     3301 ccgtccgtat caactggaga tggagggcat ccccgaaagg tacgacacgg ccggggacaa
     3361 cgaaccgggc gacgagtcga taacagggac acgtcgttgg caggccgtca gtctcgccgt
     3421 caaagcatgg tggcgaaggc agggtgacta cgctcgcctc ggcctcgtct gcgctgctgg
     3481 cctcctcatc ttctgggtct ttccgtccgc gtctggggac tacgggtggg cctctcacga
     3541 atctcgcgcc agtgaccgca ccaaattgga tgtgcttgcc tctgataatg gggaacgcca
     3601 cccactgcat ccgtggttgt gggcatggcc ggcagtgacc gaattatggt tggcccgccc
     3661 ggaggaggtg caacgagctc cagcatcgag cggccaacgg tgggtcgtgg ccaacgtaca
     3721 gaacaagtac cacgagggac tggcagagac cactgaggac tctttccaga ttgtgtacga
     3781 ctgtcagcgt ggactggcgg ctatcggccg ctactacgcc tttagcgagg cagaggccgt
     3841 gctgtactcc agcgggttgg acggcttgag ggggggcagt ccgagttgga ctccatatcg
     3901 ggaacctcac gccctgggta atccgacgcg gaacttcaag gcggcacaac ggggaacact
     3961 cctggagaag gcagggggct tcgtttgccg gaaggtggga tacagttggg cggtggagtc
     4021 tccacagaag tagatagcag atgacaggac tgcatggtgg tcgcacgaag acgcgacgaa
     4081 accgtatgtc gctagcagcc cagcaggggt gaagcattcc agggagagcg ctacggtcgg
     4141 ttgggcgact gcggctatcc ccatggggtc cggtcagttg tcctcttctg catcggtaac
     4201 ggtaacggta cccatagagt gattgctgtt gccgaccagc accacggccc aaaatggact
     4261 gcgaccgggg acacgctggg gttgatggcg cacaaaaaga aagaggaggc cagcgtcccg
     4321 ctggcccccc cgcgattagt cctgctgctg ctcctcctcc acctcgtcgt cgtagtacag
     4381 tccttcgggt tcgctactat cggtaacagt aacggtaccc atagggttgt cctggttact
     4441 gttgccaacc ggagcataga tgaactcgcg cccctgcttg cctatggatt ggacgactcc
     4501 ctggcgctgc agccggttca cctcggtctt caccgaacca cgattcttgt tgagggtggc
     4561 ggctatcgcc gtgaccgtag tcggcccctg tttgcgcaga gcatccgcaa ccctcttctg
     4621 cagattggcc gattcttcga tgggccaggc agccgggttt gcctccacca tctggaccat
     4681 gtcgagactg ccgtcttcat cctccaggaa cacgacctcc aactggatag gctcggactt
     4741 cggccccatg ttggtcttgt ggtggagcag cgttaccatc gccttgccat ccttactacc
     4801 ctccaacacg gcgtgccata ccatccggca gaggttctct ttgtagacgc tgccgtaagc
     4861 ggtcttgtgc ttgcggtcct gccccacctg aactttggcc tcatggtcga taatcagcag
     4921 cccacccgct ccgatggagc gcagcccgcg catgaacgcg attaccggct cctcgtcttt
     4981 ggcatcgccg gtgctcgcca tgccgtagct gtcgattatc agcaggccgc cggggttaga
     5041 gtccagccac tcttccacct tcgactgcac ctgctccatg gtgtggccga cacatgggaa
     5101 gtatgcgaaa tcctccggca cgtcctccag gtccaggccc ttggtgacgg ccagcgcgcg
     5161 gatgtgctgt tcctgctggt cgaactcgaa gtcgagatag gcgaccgggc gcttgcttac
     5221 ctctctcccc aaccagggca tgccctcggc cacgcagacc cccatggtca acgccaccat
     5281 gctcttagtg ctgcctccgg cgccgtacag catcgcgggc aacccttccg gcacgaactg
     5341 cgcgacgacc cagcaacggg gctttggcct ggggagcccc ttgaagtaga ccggctcaac
     5401 ggcaacagcg tcatccctat aggtaccgtt actgttacct ttgggactcg gaccttcccc
     5461 gccgggggca ctagtgccgc cgtcggggtt cacgccctgg ggttgtgaat cgctggcggg
     5521 cactccccag agcttcgcgg cttctgcttt ggtgatggtg tgtcccctgg cgcagatata
     5581 cctgtccgaa tgcgtttcca ggattgccgc cccgcaccgg caccgtcgtt ccaggaaggg
     5641 cagcaaggcg accgcttcac tgctgtccgc cactacgcag cctccccggc gggagtcact
     5701 ttccagtctc ggagcagaag gccccgctgc tggccgaaac ccctattaac gtcgaagccc
     5761 cgcctgtcga agtcggcaag gctcgcctcc cacacctgcc cgcgttcgtc taccaaccga
     5821 acgccccgcc cgttcatggc cgccaactgc tccaggtggt tggcatcgac cgcccaggcc
     5881 ggaggcttgc ggagcatatg cctggtggtg ctaacccgct tgagtaggtg gccgtccgca
     5941 gtcacgcttc ccacgctgcg gccgtccagg ttgtagaact gcctccgcgt gttagaatgg
     6001 gcgctgcgac catcattggt cgtgtcggcc gccagcaccg cactccttcc ccaaggccgg
     6061 ttgctggcgg tttctcttac ctgctccacg gctgcacctc gtcagcgggc catacggccc
     6121 gtcgcttcgc ttccgcccgc accaggtgtc tgaggacggc agccaaagac accccggcct
     6181 gagcagacaa ccggcacagc gcagcgtact ccctgggatt gaggacgaaa ccgagacgct
     6241 taatcctgta ctcttccatg tccacctcgt ctacctcctt tctcagtaca agtagctggg
     6301 gctactctga caccagagta gccccagcta tcggaaggtt tcggaaggat aaacggaaga
     6361 ttcggaaggt ttcggaaggc tatacgggtc ggtccagccg tccctccttc cccgccctga
     6421 tgatgtagcc tagcgtcttg gtagatacct tttccttcgg atggagcctt ccaagccagt
     6481 ctgcgatggc ggcaatcttt ttcccgtcca ttgcctgctt accaaccagt tgccaaatgc
     6541 gatgccagcg aaggtagtcc ggtcccctcg ccgggactcc tacctcgtat cctggtatgg
     6601 gggcatctgg tgtattggcg ccccctcctg tgctctggtt gctatctcca attgcggcag
     6661 cggtgcgata cgcagacacg ccttccgccg tctcggggaa gtcccgctcc atttcgccaa
     6721 gtagtccaga gaacaggtcc agcagggccc tattacgaca gtaccccagc cctttggagc
     6781 ggccacgccc cagagatagg acctctatct caatgacatc tggaagctca ttgagtcggg
     6841 ggtgttgctc cctcaactcc ctcgcatatc gctccgcctc ggtctcgtca gtggatgggg
     6901 catccggcag atacaacccg cagatggaca ggcggtaact gctgggcgtg tggtaggtaa
     6961 caagcccaga cagcgttacc ttccctcctg gcacctggaa ggtactgcct tccatccccc
     7021 gcgtgtggta cccaaaccac gcgaccacta catgggcgtc tgcgttgacg cggagagtca
     7081 caaacggcag gcgctggtcg atgatgccaa agcctcgggg caaacgcatg gaatcggtcc
     7141 acctcctgca tggtggttgg gtgtgccgtg ctccggcgtg caggcgtcgg acttgcggga
     7201 cgggccaggt ccacacggcg aacccattct acactacctg cttagcgcct cctccatcat
     7261 cgtggctgcc tgccggtcct ggtgcttcat ggcgtgggcg tatatggtca tagtgatatt
     7321 ggggctggcg tggcccaggc gggccgagac tgccggaacg gggaggccct tgctaaggag
     7381 tagggtagcc gccatgtggc gcaatccgtg gggcgtgagc ggcggcaggc ccaactctca
     7441 acctctcggt gcatggcctt ctgcacggtg gactgggcga ggggttgccc gtactcaccc
     7501 gtgaatacca acccggtgtc ctgccactgg gggccagcct tcaaccgcca ttctgcctgt
     7561 tgcgcccttt gtcccttcag tgcggctacg gctgcggctg gaagtaccat cgtccggtcc
     7621 cctgccctgg tctttggggt ggactccacc cactccccag cgatgcgatg caaggagcgg
     7681 cagacccgca gggcggccgc cgcaaggtcc acgtcctgcc agcgtagccc ctgtagttcc
     7741 gacaagcggc agccggtaga tgcggccgtc acccacaggg gatgcaacca tcggcccctg
     7801 gtcccctcca ggaactgttg cagttcatcc acgctccaca tttccttacg gctggcctgg
     7861 tgcttggggc gaatcacccg gtcacagggg ttctgcgcca gccaccccca gagcaccgcc
     7921 atcttgcagg cccggtgcag catggcgtgg ccgtactggg cttcccgttc ggtgccgcag
     7981 gaaagcagtt tcgagtagaa cgcctgcacc ttttcgggag acagcttgcc gagcctaacg
     8041 tggccgagaa cggggcggag gtggcgtttg catcgggcct gatagtcgtg gaaggtcctg
     8101 ggcttgaggc tgggccttgc ggtttgcagc cacaggtcca gcaggtcgtt gaccgtcctg
     8161 ttgccggggg tggcaagggt gccggtttgg ccacactccc ttagcagggc ggcaaggata
     8221 gccctagcct ccttctcggt cgctgcgtat ctggttatag gcttgccgtc gttgcccagt
     8281 ttggccgccc agcgcccgtc tgacttccgc tggtagaccg aacccacgcc ataggtgcgc
     8341 tcctgctttc gggacatagc ctttcctcct tgccatgtcc ccgacggtgc gaaaaaggtg
     8401 tcagatgggt gtcaggggcc tataataggc acgaaattga ggtaccggcg gacttaaaat
     8461 ccgcttcccg caaaggagta ggggttcgag tcccctcccc ggtaccggca gaagggggct
     8521 ctcagcaaag gctgggggcc tttttctttt gcgctgttcc tctcgagtgt tgca
//
LOCUS       DATALS010000172        11911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_52509,
            whole genome shotgun sequence.
ACCESSION   DATALS010000172 DATALS010000000
VERSION     DATALS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11911
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52509"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VHS06_03730"
     CDS             <1..1297
                     /locus_tag="VHS06_03730"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HEX2987262.1"
                     /translation="QIESFATLFMGGVGLSVMEEIPLNLENVVRALGGQILVEGNGKR
                     LWDKVFVGAMSLESMELRIAPDSLMVMGDRSDAQRMAIERGISAIVVTGGFEVSPEVL
                     ELARKHQVSIITSRHHTFKTVQLLNLSIPVRHVMTSDVLTAEEDELLDDARPLLAKQP
                     ELPVVDAEGKVVGVVSRSSILRPARQKVALVDHNERSQSVEGLEEAEIVSVVDHHRIY
                     DIRTDTPIFVRCEPLGSSNTIIYKMCRENMVQIPKSIAGIMCGAILTDTIMFKSPTST
                     QEDRRAAEVLAERAGVDLNELGKAVLSAASDVAGRAPRELLLGDFKEFALGNLRFGVS
                     SIETVNASAVAAMRDELLDQMRVLREERRYASVILMVMDIAHEHTEMLVDGHEREVAE
                     AFDRPVEDGHKIEFPGILSRKKQIVPLLPAIRDNIASRA"
     gene            complement(1504..2130)
                     /locus_tag="VHS06_03735"
     CDS             complement(1504..2130)
                     /locus_tag="VHS06_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2987263.1"
                     /translation="MPDLHPSVSRLVLGPLQTNCYFITCPETGESLVIDPADDAERIA
                     GAVKGSGGTIRQILLTHVHPDHIGGLLGLRDRTGARLMVHQLDVQLLQSQGGFFGVRP
                     ADVAKMAPDQQLQGGEEITVGHLTGSIIHTPGHTPGGITLRMGNLLFTGDTLFAQGVG
                     RVDLPGGSLDALMNSIRGLFTLPDDCVVYPGHGPSSTIGTEKRDNPYV"
     gene            2208..4103
                     /locus_tag="VHS06_03740"
     CDS             2208..4103
                     /locus_tag="VHS06_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987264.1"
                     /translation="MRLPDALLECTLPLLRKIALNHSLPVGDDTLRPELAGQLAEMLS
                     KPGYLERCVERLDEGERRILGEVAGSRWCAKAFVIDRLSPRKGGTTSSSKREPLSPCL
                     SLLQKGLLYRGFGIIGDWRGELYYVPDEMQSAFAPLAARSTKEAAPDVRSASVPPSVE
                     EHSASFDLFCLLSYLRRQHCRLRGDRLARSDAERLDVECGRRTEVWSAGRAEDRWGFL
                     LQLCLSLGWVHREGHSLTLSQTAFRALAGDVTSVRTRLIESYLANRFWSDLEAAGRVR
                     QLLGGRRIDEASARKVALHYLDTLCGENWCDDAAFCEAVRSTNPDFLREDYSSPGWAV
                     IDTRSGIELSGEGSWNAVEGEWLRYLLLGPLFWLGFVRWGTSPGGLPIAFQFCGTPQQ
                     VVTESAAIQESGEELRVTAPAQCDTTRLFQLERYAELQKRAGSSSYRLGRDTVLSAME
                     SGGSREELVRLLRDLSGDEIDGVLLARASGWASDYGRFTLEAATLLTAENTEDSVWAE
                     SLPGVASWLGERAGGTSQKVIPEHLWDIVEALKRANQLPRVDPSLRRQGLRRIAADTE
                     LFREALFALLLVRAIQPGGQWGDAAAAIRKIESALGPEEAAKVRARVEDVTRQGDGVR
                     GMARRDR"
     gene            4185..4571
                     /gene="rplS"
                     /locus_tag="VHS06_03745"
     CDS             4185..4571
                     /gene="rplS"
                     /locus_tag="VHS06_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019543774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX2987265.1"
                     /translation="MNPSQALNVERNPNIPDFGPGDTVKVGVKVVEGGRERLQNFEGV
                     VIVVSKPKQPDGTFTVRRVTHGVGVERTFAFASPRVDRVDVMRRGAVRRARLYYLRAL
                     VGKAARIKEKRPAMAARRSSAEGEKA"
     gene            4587..5255
                     /locus_tag="VHS06_03750"
     CDS             4587..5255
                     /locus_tag="VHS06_03750"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX2987266.1"
                     /translation="MRPRTSPHYSSPESPTLVEEHSLASQGFCRIAGLDEAGRGSWAG
                     PMVTAAVCLPLHRDGLAQQLDGVRDSKQLSPAARDRLFGRVIECAEDVGIGVVPPETI
                     DKLGLTRAWELAMTWALDDMALRPDHLLVDAFLIKTCPLPQRPLIHGDCISLSIAAAS
                     IIAKVTRDRMMVAADLVYPGYGFAIHKGYGTAAHRRALEAGGPCAYHRRSFAPLRNEW
                     RLPL"
     gene            5252..5632
                     /locus_tag="VHS06_03755"
     CDS             5252..5632
                     /locus_tag="VHS06_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX2987267.1"
                     /translation="MTDQRHRDGRAGEQLAAEHLAAGGYRLLDRNYRCSLGELDLVAE
                     RKGELVFVEVRTKRQPCLFRPEESISRDKALRLMRLAEYYMAATGQEDRPWRIDVVAV
                     ELRPDGSKARLEHFANATAAMELE"
     gene            5629..6741
                     /locus_tag="VHS06_03760"
     CDS             5629..6741
                     /locus_tag="VHS06_03760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX2987268.1"
                     /translation="MSRGLLRLLAVLLLLLSALATAGFGPPTLVESDDAASPVSPSIL
                     KQMRLDRQPQLSAKAAVILDVTSGQFILEKNSHSRRAPASTTKMTTALVTLENARLSD
                     VVTAGPNVKVEPSIIGLDPGDKLTVEQLLYGLLLPSGNDAAVALAEHVGGTQAAFATM
                     MNAKVAQLGLEDSRYVNPHGLDAAGHYSSAYDLALTARAAFENPVFEKIVATREYRIE
                     SPVKWYFKNNNRLLDTFLGADGVKTGYTDDAGRCLVASATRGGHRIITVVLDSLDTVA
                     ESAALLDYAFSNYSWEPIRAGDSPLGVYGQGTEMRRLVAAPQPDMIIANWQRPYLRWY
                     FSVPSGQGQGDGTSGRITYYLFGRKVAEFQLRGDQR"
     gene            6796..7569
                     /locus_tag="VHS06_03765"
     CDS             6796..7569
                     /locus_tag="VHS06_03765"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX2987269.1"
                     /translation="MAEASIPPQDSSSSNGDNGRLERLHKVLAHAGLESRRAAEEMIA
                     AGRVSVNGKVITTLGTKVDPERDVITVDNKPIPRRVKKIYLMLNKPPGYITTVNDPEH
                     RPTVMELVPHSSRIYPVGRLDANSEGLILMTNDGEFANLLAHPRYSYEKEYHVAVPGT
                     VSEEDLRALREGVEIEGGRTSPAQVRVLSSDGKVTWLSMTIHEGRNRQIRRMLHALGY
                     RVERLVRMRVGPLWLGSLPRGSYRYLTPAEIRSLKRRGA"
     gene            7566..8276
                     /gene="cmk"
                     /locus_tag="VHS06_03770"
     CDS             7566..8276
                     /gene="cmk"
                     /locus_tag="VHS06_03770"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006319403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX2987270.1"
                     /translation="MKAPVICMDGPAASGKTTIAKMLAERLGYFYLDTGVLYRAVTWV
                     ALQQGVSPSDGEKLAELASSIEIEVKPAPPGDVRQTIVLADGEDVSLAIRTAPVDAHV
                     SEVSAHPAVRTALIGIQRSVAAKGGVILAGRDAGTVVWPESEVKLFLVASEQERARRR
                     LEQARSQGIEVDFDTVLAELRRRDKYDSSRAVAPLKAATDAIVLDTTNLSIEQVVNEA
                     LRIVREKSGAGLDRRGIL"
     gene            8292..8936
                     /locus_tag="VHS06_03775"
     CDS             8292..8936
                     /locus_tag="VHS06_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2987271.1"
                     /translation="MFYRFACLVVLGILRLFTRCRVEGLENVPAGGPLLLVCNHLNMV
                     DPPLLGAIFPRRIVFMAKDESFHHAFMGPLVKWYGAFPVRRGQPDRQALRTAESVLRG
                     DGVVGMFPEGHRSKTGKMQRAFPGASLLAIMAAAPVLPVALEGTDQLKSPFALLARPT
                     ITVRIGKPFQLQRGEDRQKVDLATLTDHMMVRVAALLPEEYRGYYSNAFQGERK"
     gene            8960..10282
                     /locus_tag="VHS06_03780"
     CDS             8960..10282
                     /locus_tag="VHS06_03780"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX2987272.1"
                     /translation="MMMQNEIGPVGGVVSSVDPGSIAEACGMLPGDRLLEVNGCALRD
                     VIDYRYASGSDDLTLLFLRGEEEHEVEVERDWEDELGIQFETPIFDRIRQCRNRCCFC
                     FVKNLPKGMRSSLYIRDDDYRLSFLFGNFVTLSNVDVSDLERIAEQRLSPLYVSVHAT
                     DRTVRNSLLGVDAPDILSQIEDLGRRGVRIHAQIVLCPGINDGEQLTATIEDLAARQD
                     TVLSAAVVPIGLTRFCQNREMRLFSPSEARGVVERVTPLQKRFKASIGRRFVHLSDEF
                     YVMSGIPVPPASWYDGFPQLDNGVGMVRRLLSSWASSRRRLPAKLDSPRRVGWICGTS
                     AYPTLSLLAADIMNRVDGLHVELHQVQNRFFGTSVTVSGLLTGADVLDTIKQKHLDCW
                     VLPRVMFDDAGDRTLDGVTLKEIRETVPDPVRLADSASELMRLSVRGE"
     gene            10290..>11911
                     /gene="glmS"
                     /locus_tag="VHS06_03785"
     CDS             10290..>11911
                     /gene="glmS"
                     /locus_tag="VHS06_03785"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX2987273.1"
                     /translation="MCGIVGYTGKADVLPILMDGLRRLEYRGYDSAGVAVITPDHELS
                     IHRAAGKLVNLARELEGSEPRGFTGIGHTRWATHGRPSTENAHPHTDCSGRLCVVHNG
                     IIENYTTIRRGLLERGHHFDSETDTEVLAHLIEEELARLEKLGEDGSGSRLLSAVRRA
                     LLQVRGAYAITVLSLDEPDVVVGARQNAPLIVGLGEGENYLASDIPAVLHRTRRIIRL
                     GEGELALLTPQSVSIFDAVGKRVDARVDEVVWDHGAAEKGGYPHFVLKEINEQPEAVT
                     NAMVGRLGRPEAQLAEMEKIDLSDVNRIVLAACGTSLYACMIAKYAFEEWTRIPVEVA
                     VASEFRYADPIMDSHTLFVAVSQSGETADTLAAFKQAKEMGAKTVSVANVVGSAITVG
                     ADAVLYLQVGPEIGVVATKTFTGQLAVLMLLGLHVARSRGTISSEQVEQISRDLAVVP
                     EAMRKALATDQEASRVAYSVANVAHLLFLGRGVGYPTAMEGALKLKEISYIHAEGYPA
                     GELKHGPIALLDPSMPILAVATESRTYEKLVS"
BASE COUNT         2316 a         3459 c         3854 g         2282 t
ORIGIN      
        1 ccagatcgag agcttcgcca cgctgtttat gggtggagtc gggctctctg tgatggagga
       61 gatccctcta aaccttgaga acgtggtcag ggcactgggt ggacagatcc ttgtcgaagg
      121 gaacgggaaa cgactctggg acaaggtctt cgtcggagcc atgagcctgg agtcgatgga
      181 gctgcggatc gcccccgact ctctcatggt aatgggcgat agaagtgacg ctcagaggat
      241 ggcgatcgag cggggcatca gcgcaatcgt ggtcaccggt ggcttcgagg tcagccccga
      301 agtgttggag ctggcccgca agcatcaggt ttccataatc acgtctcgcc atcatacctt
      361 taagacggtc cagcttctca atctcagcat tcctgtccgc cacgtgatga cctccgatgt
      421 gctcactgca gaggaggacg aactcctgga cgacgctcgg ccattgttgg ccaagcagcc
      481 ggaacttccg gtcgtagatg ccgagggaaa ggtcgtcggg gtggtttccc gaagcagcat
      541 cctcaggccg gcacggcaga aagtcgcgct tgtggaccac aacgagcgaa gccagtcggt
      601 ggaaggcctg gaggaagccg agatcgtctc tgtcgtggac caccatcgga tatacgacat
      661 ccggaccgat actcctatct tcgtacggtg cgagccgctg ggctccagca acaccatcat
      721 ctataagatg tgccgcgaga acatggtgca gattcctaag tccatcgcgg ggatcatgtg
      781 cggagccatt ctgacggaca ccatcatgtt caagtctcct acctctactc aggaggaccg
      841 gcgagctgcc gaggtattgg ccgagcgggc aggggtagac ctgaacgaac tgggaaaggc
      901 cgtgctctca gcagcctccg acgtagcggg aagggccccg agagaattgc tgctgggcga
      961 cttcaaagag ttcgcgctgg gcaacctgcg cttcggtgtc tcaagcatcg agacggtcaa
     1021 tgcatcggcc gtcgctgcaa tgcgggacga gctacttgac cagatgagag tgctgcgaga
     1081 ggaacggcgc tacgccagcg ttatcctgat ggttatggac atagctcacg agcacacgga
     1141 gatgctggtc gacggtcacg agagggaagt tgcggaggcc ttcgatcgcc cggtcgagga
     1201 cggccacaag atagagttcc cggggatact ctctcgaaag aaacaaatcg tccccttgtt
     1261 gcccgccatc cgggacaaca tcgcctcgcg ggcctaacag ctacctggca gtacgcaaaa
     1321 gcgggtggac atcagatcgg ccttgtggaa cggcacctcg agattgagac gtagggcacg
     1381 gggcgcgagc gagcacagcg ccccgcttgc gcccgccatc agcgtgatcg taggcaccgt
     1441 ggtggtcgtg gcagcggtgt ggggggcggg gcacaagacc ccgccctacg acgtttccgg
     1501 aagctacacg tatgggttgt ccctcttctc ggttccaatc gtggaggagg gaccgtggcc
     1561 cgggtacacg acacaatcgt ccggcagcgt gaacagtccg cgtatgctgt tcatcagggc
     1621 gtccaggctc cccccgggca ggtcgaccct gcccacccct tgcgcgaaca gggtgtcgcc
     1681 ggtgaagagc agattgccca ttctgagggt gataccgccg ggggtgtgtc ccggcgtatg
     1741 gatgatgctt cccgtcaggt ggccgacggt aatctcttcg ccaccctgca actgctgatc
     1801 aggcgccatc tttgccacgt cggcaggtcg aacgccgaag aacccgccct gactctgtag
     1861 cagctgcacg tctagctggt ggaccattaa cctggcgccc gtccgatccc gcaggccgag
     1921 caacccacct atgtggtccg ggtgcacgtg agtaagaaga atctggcgga tcgtgccgcc
     1981 gctgcccttg acggcacctg ctatccgctc agcgtcgtcg gcggggtcga tgaccaggct
     2041 ttcccccgtt tccgggcagg tgataaagta gcagttggtc tgaaggggcc ccaacacgag
     2101 cctggagact gatggatgga gatctggcat agttcatcac ccccaattgc cggattgtgc
     2161 cgctattctc tagcgggcga tctacctcag tcaaggacaa gatgccgttg agactgccgg
     2221 atgcgcttct ggagtgcaca ctgccgcttc tgcgaaagat tgccctcaac cactctctgc
     2281 ctgttggcga cgacacgctg cgcccggagt tggccggcca gctagcggag atgctgtcga
     2341 agcccggcta tttggagcga tgcgtggagc ggctggacga gggcgagcgt cgcatcctcg
     2401 gagaggtcgc aggcagcagg tggtgcgcga aagccttcgt gatcgacagg ctgagcccaa
     2461 gaaagggagg caccacctcc tccagtaagc gagagccgct cagcccctgt ctctccctcc
     2521 tccagaaggg gttgctctac aggggtttcg ggatcattgg ggactggcga ggggagctgt
     2581 attacgtgcc ggacgagatg cagtccgctt ttgcccccct tgccgcccga tccaccaaag
     2641 aggccgcgcc ggacgtaagg tcggcctcgg ttccgccctc cgtcgaagaa cactcggcaa
     2701 gcttcgacct cttctgcctt ctctcctatc tacgccgtca gcactgccgt ctccgggggg
     2761 acagactggc gaggagcgac gcggagcggc tggacgtgga atgcggacgc aggaccgagg
     2821 tgtggagcgc cggcagggca gaggatcgat ggggctttct tcttcagttg tgtctctcac
     2881 tcggctgggt gcacagggag gggcacagcc tcaccctcag ccaaaccgcg tttcgggcac
     2941 tcgccggaga tgtcacgtcg gtgcggaccc gactcataga gtcctatctt gcgaaccgct
     3001 tctggagtga cctggaggcc gctgggcgag tccgccaact cctcggaggc cgtcgcatcg
     3061 acgaggcatc cgcaagaaag gtcgcccttc actacctcga tactctttgt ggggagaact
     3121 ggtgcgacga cgcggccttc tgtgaggcag ttcgctccac aaacccagac tttctccgcg
     3181 aagactacag ttctcccggg tgggccgtca tcgacactag atccggcatc gagctgtccg
     3241 gcgaaggaag ttggaatgcc gtggaggggg agtggctaag gtaccttctc ctcggccccc
     3301 tcttctggtt gggtttcgtc cgttggggca cttctcctgg gggattgccc atcgcgtttc
     3361 agttctgcgg caccccacag caggtcgtga ccgagtccgc agccatccag gaatccggcg
     3421 aggagttgag ggtgacggcc ccagcccaat gcgacaccac aaggctgttt caactggagc
     3481 gttacgcgga actgcagaag cgggcaggtt cttccagcta ccggctaggc agagacaccg
     3541 tcttgagcgc catggagtcg ggaggatcga gagaagagtt ggtccggctg ctgcgcgacc
     3601 tgagcggcga cgagatcgac ggtgtgctgc ttgcgagggc atctgggtgg gcctccgact
     3661 atgggcgctt cacactcgag gcggccacac tcctgaccgc ggagaacacg gaggactccg
     3721 tctgggcgga gagcctgcca ggggttgcat cctggctggg tgagagggca gggggcacca
     3781 gccagaaggt aattcccgaa cacctgtggg acatcgtcga ggcactcaag agggcaaacc
     3841 aactcccgcg cgtcgaccca agtctgcgaa gacagggact tcggcgcatt gccgcggata
     3901 cggaactgtt tagagaggcg ctctttgcgc tgctgttggt tcgagcgata caacccggcg
     3961 gtcaatgggg ggatgctgcc gcggcgattc gaaagatcga gtcggcactg gggccggagg
     4021 aggcagcgaa ggttcgcgcc cgggtcgaag atgtgacacg gcaaggggat ggggtgcgag
     4081 gaatggctcg cagggatcgc taactgcggt tgttgggcac gctatcgccg tgatataatg
     4141 ctccagtctt tgtggcaatt gaactggcca gggggtaggc agtgatcaat cccagtcaag
     4201 ctctgaacgt tgagcgaaat cccaacatac cggactttgg gcccggggat accgtaaagg
     4261 ttggcgtcaa ggtcgttgag ggtggccgag agcggcttca gaacttcgaa ggcgtggtca
     4321 tcgtcgtctc gaagccgaag cagcccgacg ggactttcac cgttcgacgc gtcacccacg
     4381 gagtgggagt agagcgaacc ttcgcgtttg cctctcctcg cgtcgatcgt gtcgacgtga
     4441 tgcgccgtgg tgccgttcgg cgcgctcgcc tctactacct cagggcactc gtgggcaagg
     4501 ctgcacgcat caaggagaag cggcctgcga tggcagcccg tcgttcgtcc gctgagggcg
     4561 aaaaggctta aggtgcgggg gtgcatgtgc gtccccgcac ttctccccat tactcatccc
     4621 cggaatctcc cactcttgtt gaagagcact ctctggcatc ccaggggttc tgtagaatag
     4681 ccggactgga cgaggccggc agagggtcct gggctggtcc gatggtgacg gctgccgtgt
     4741 gccttcctct gcaccgggat ggtctcgcgc agcagttgga cggcgtccgc gactcgaagc
     4801 aactctcgcc ggccgcccga gaccggctct tcggcagggt tattgaatgc gccgaggacg
     4861 tgggtatcgg cgtggtaccg ccggaaacca tcgacaagtt gggtttgaca cgcgcctggg
     4921 agctggcaat gacctgggcg ctagatgata tggcactgcg tcccgaccac ctcctagtgg
     4981 acgcctttct catcaagact tgccctctac ctcaacgccc cttgatccac ggcgactgca
     5041 tcagtctctc catcgcggcg gcgtctatca tagccaaggt aactcgagac cggatgatgg
     5101 tggcggcgga tctcgtgtac cccggatacg gtttcgccat tcacaagggc tatggcactg
     5161 ccgctcacag gcgagctctg gaggcgggcg gaccctgcgc ttaccaccgg cgctcctttg
     5221 cccctttgcg gaacgagtgg aggttgccgc tgtgacggac caacgacacc gagacggccg
     5281 cgccggagag caactggcgg cagagcacct cgccgctgga ggctacaggc tgctggatcg
     5341 caactaccgc tgctcactgg gtgagttgga tctggtggct gagcggaagg gagagctggt
     5401 gttcgtcgag gtgagaacga agcgccaacc ctgtcttttt cgccccgagg agtctatctc
     5461 ccgcgacaag gcacttcgcc tgatgcggct cgcagagtac tatatggcgg cgactggaca
     5521 ggaagatcgt ccgtggagaa tagacgtggt tgccgtagag ctgcgtcccg atggtagcaa
     5581 ggcacgcctg gagcacttcg ctaatgccac ggcggcaatg gagttggaat gagtcgaggt
     5641 ctccttcgcc ttctagcagt tctactgttg ctgctgtcgg cgctggcaac agcaggcttc
     5701 ggtcccccca ctctcgtcga atccgacgat gccgcctccc cggtatcccc ctccattctc
     5761 aagcagatga ggctggaccg gcagccacag ctgtccgcga aagcggcggt gatactggat
     5821 gtcacctccg gacagttcat cttggagaag aactcgcact ctcgcagggc ccccgccagc
     5881 accacaaaga tgaccaccgc actggtgacg ctggagaatg ctcgcctgtc cgacgtcgta
     5941 acggctggac ccaacgtcaa ggtagagccg tccatcattg ggttggaccc aggggacaag
     6001 ctgacggtcg agcaactgtt gtacggcctg ctgctcccct cgggcaacga tgccgcggtc
     6061 gcgctggcag agcatgtggg gggcactcag gccgcctttg ctacgatgat gaacgccaag
     6121 gtcgctcaac tggggctaga ggatagtcgc tacgtgaacc cgcacgggct ggacgctgcc
     6181 ggacactact cgtcggccta cgatctcgcc ctgaccgcgc gtgcggcctt cgagaacccg
     6241 gtcttcgaga agattgtcgc taccagggag tatcgcatag aaagccctgt gaaatggtac
     6301 tttaagaaca acaatcgact tttggatacc tttcttggtg cggatggcgt aaagacgggg
     6361 tatactgatg acgcaggaag gtgtctggtg gcttctgcca cgcggggtgg gcataggatc
     6421 atcaccgtgg tcctggacag cctggacact gttgcagagt cggcagcctt gctcgactac
     6481 gcgttctcca actacagctg ggagccgata agggccgggg acagcccgtt gggagtgtac
     6541 ggtcagggca ccgagatgcg acggttggtg gcagcacctc aacccgatat gatcatagcg
     6601 aactggcaac gaccttactt gcgctggtat ttctcggtgc cgtctggtca gggacaaggg
     6661 gatggcacct cgggaaggat tacctactat ctgttcggcc gaaaggtcgc ggagttccag
     6721 ctacgcggcg accaacggtg aagcccgggg ggccgtcgcc aaggagcaat agacaactgg
     6781 cgcgcgtggg agattatggc tgaggcatca attccaccac aagattcttc gtcctccaat
     6841 ggagacaacg gcaggctcga acgactgcac aaggtgctgg ctcacgctgg actggagtct
     6901 cgccgcgcgg ccgaggagat gatagcggcc ggcagggttt ccgtgaacgg caaggtgata
     6961 accacgctgg gcacaaaggt ggaccctgag cgcgacgtga taacggtgga caacaagccg
     7021 atccctcgcc gcgtaaagaa gatctacctc atgctgaaca agccccccgg ctacatcacc
     7081 acggtgaacg atcccgagca tcggcctacg gtgatggagt tggtgccgca cagctcgcgg
     7141 atctaccccg tggggaggct ggacgccaac agcgaggggt tgatcctgat gaccaatgac
     7201 ggggagtttg ccaacctcct tgcgcaccct cgatattcct acgagaagga gtatcacgtg
     7261 gccgtgccgg gcacggtgag cgaagaagac ttgcgggcgc tccgggaagg ggtcgagata
     7321 gagggcgggc gcacttcacc ggctcaggtg agggtgctga gctccgacgg gaaagtgacc
     7381 tggttgagca tgaccatcca cgagggacga aaccggcaga tacgtcgcat gctgcacgcc
     7441 ctgggatatc gagtggagcg cttggtgcga atgcgtgtgg gtcccctctg gctcggctca
     7501 ctgcccaggg gatcatacag gtatctgact ccggcagaga taaggagtct caagaggagg
     7561 ggggcgtgaa ggctccggtg atatgcatgg acggccccgc ggcctccggc aagaccacca
     7621 ttgcgaagat gctggcagag cggctcggct acttctacct cgacacgggc gtcctttacc
     7681 gagccgtcac ctgggtcgcc ttacagcaag gggtatcccc atccgatggt gagaagctcg
     7741 cggaactggc ctcgtccatc gagattgagg taaagccggc gccgcccggc gacgtccgac
     7801 agactatcgt cctcgccgat ggagaagacg tctcacttgc cataaggact gcccctgtgg
     7861 acgcccacgt gtcggaggtc tcggcccacc ctgccgtgcg cacggcgctc atcggtatcc
     7921 agcgttctgt cgcggcgaag ggtggggtta tcctggctgg gcgagatgcc ggcactgtgg
     7981 tctggcccga gtccgaggtg aagctcttcc tcgttgccag cgagcaggag agggctagga
     8041 gaaggctgga gcaggccagg agtcaaggaa tcgaggtgga tttcgataca gtccttgcgg
     8101 agttgcgccg gcgcgacaag tacgacagca gcagggccgt tgcacctctc aaggccgcca
     8161 cggacgccat cgtcctcgac accaccaact tgagcatcga gcaggtggtt aacgaggcgc
     8221 tccgaatcgt gcgagagaag agcggcgctg ggctcgaccg gcgtggcatc ctctagacag
     8281 gacccctcat catgttctat cgcttcgcct gccttgttgt cttgggcatt ttgcggcttt
     8341 tcacccgatg ccgagtggag ggtctggaga atgtccccgc cggaggcccg ctgctgctgg
     8401 tctgcaatca ccttaatatg gtcgaccccc ctctcctcgg cgccatcttc cccagacgga
     8461 tagtcttcat ggcgaaggat gagtcgttcc accacgcctt catgggcccc ctggtgaagt
     8521 ggtatggcgc cttccccgtg cgtcgggggc agcccgaccg ccaggcgctc agaaccgccg
     8581 agtcggtgtt gcggggcgac ggtgttgtag gaatgtttcc ggaggggcat cgcagcaaga
     8641 ccggcaagat gcagcgcgct ttccccggcg catccctgct ggccatcatg gcggctgcgc
     8701 ccgtcttgcc cgtagcactg gagggaacgg atcaactgaa atcgccattt gccctgctcg
     8761 cccgtcccac catcacagtg cgaattggaa aacccttcca gttgcagcgg ggcgaagata
     8821 ggcagaaggt cgatctggca accctcacgg accatatgat ggtgagggtt gccgcgctgt
     8881 tgcccgagga gtacagaggc tactactcga acgcattcca gggtgagcgg aagtagctcc
     8941 ctctgttaaa ctggcactca tgatgatgca gaacgaaatc ggccccgtgg gcggggttgt
     9001 ctcttccgtg gatcccggca gcatcgccga ggcgtgtggg atgctgccgg gcgataggct
     9061 gttagaggtc aacggttgcg cactgcggga cgtgatcgac taccgctatg cctctggatc
     9121 cgatgatctc acgttgctgt tcctacgagg agaggaagag cacgaagtcg aggtcgagag
     9181 agactgggaa gacgaactgg gaatacagtt cgagaccccg atcttcgatc ggatacgcca
     9241 gtgccgaaac cggtgctgct tctgcttcgt aaagaacctc ccgaagggca tgcgcagctc
     9301 tctttacata agggacgacg actatcgtct ctcatttctg ttcgggaatt tcgtcactct
     9361 ctccaacgtc gacgtttccg atctcgaaag gatcgccgag cagcggttga gtccgctgta
     9421 cgtttcggtc cacgccacgg accgcacggt gcggaacagc cttctagggg tcgatgcccc
     9481 ggatatcctg agtcagatcg aagacctcgg acggcgtggg gtgaggatcc atgcccagat
     9541 agtgctatgc ccggggatca acgacggcga gcaacttacc gcgaccatcg aggatctcgc
     9601 cgcaaggcaa gacacggtgc tgtcggcggc agtggttccg atcggcctca cccgcttctg
     9661 ccagaacagg gagatgcgtc tcttttctcc atccgaggca agaggggttg tcgagcgggt
     9721 cactccactc cagaagcgtt tcaaagcttc catcggcagg cggttcgtcc acctctctga
     9781 tgagttctac gtgatgtcgg gaataccggt gccgccggct tcctggtatg acggtttccc
     9841 gcagttggat aacggcgtcg gaatggtacg tcgattgctt tcttcttggg ccagttctcg
     9901 gcgccggctt ccggccaagc tggatagtcc aaggcgggtg ggctggattt gcgggacttc
     9961 ggcctatccg acgctttctc ttctcgcggc ggatatcatg aatcgggtcg acgggcttca
    10021 tgtggagttg caccaggtgc agaaccggtt cttcggaacg tccgtcactg tatcggggtt
    10081 gctgacaggg gccgacgtac tggatacgat caaacagaag catcttgatt gctgggtcct
    10141 tcccagggtg atgttcgacg atgcaggcga tcgcaccctg gacggagtga cgcttaagga
    10201 gattcgcgaa acggttcccg acccggtcag actcgcggac tctgcgtccg agttgatgag
    10261 actcagcgtt aggggggagt gagcaggcca tgtgcggaat tgttggctat acgggcaagg
    10321 ctgacgtatt gccgatcctg atggacggac ttcggcgttt agagtacagg ggatacgatt
    10381 ctgccggggt ggccgtgatt accccggatc acgagttgtc gatccatagg gccgccggaa
    10441 aactggtaaa cctggccagg gaactcgagg gatcggagcc tcggggtttc accggcatag
    10501 gtcacacccg ctgggcgacc catggccgcc cttccacgga aaacgcacac ccccataccg
    10561 attgcagcgg acggctctgt gtggttcaca acgggatcat cgagaactac accaccatca
    10621 ggagagggtt gctggagagg ggccatcact tcgacagtga gacagacacc gaggttctgg
    10681 cccatctcat cgaggaggaa ctggcccgcc tcgagaagct gggcgaggat ggaagcggct
    10741 cgcggctgct ctctgccgtc cggcgggcgc tgttacaggt tcgcggcgct tacgccatca
    10801 cagtactcag cctggacgaa ccggacgtgg tagttggcgc gaggcagaac gctcccttga
    10861 tcgtggggct gggtgagggt gagaactacc ttgcatccga tatccccgcc gttctccaca
    10921 gaacccgacg catcatccgt ctgggcgagg gggaactggc gctgttgaca ccccagtcgg
    10981 tgtcaatctt tgatgcggtt ggcaagcggg tcgatgcccg ggtagacgag gtggtatggg
    11041 atcacggagc ggcggaaaag ggcggttatc cgcacttcgt cctgaaggag atcaacgagc
    11101 agccagaagc agtcactaac gccatggttg gtcggctcgg ccggcccgag gcgcagctgg
    11161 ccgagatgga aaagatcgat ctgtctgacg tcaaccgcat cgtcctcgcc gcctgtggga
    11221 cctcactgta tgcctgcatg atcgcaaagt atgccttcga ggagtggacc cgcatcccgg
    11281 tggaagttgc cgtcgcctcc gaattccgct acgccgatcc catcatggat agccacaccc
    11341 tcttcgttgc ggtgtcccag tcgggagaga cggctgatac gttggccgcc ttcaagcagg
    11401 cgaaggagat gggtgcaaag accgtctcag tggccaacgt ggtgggtagc gcgataactg
    11461 tgggcgcaga cgcggtgcta tacctccagg tcggtccgga gatcggcgtc gtcgccacca
    11521 agaccttcac cggacagctg gcagtgctca tgctgctggg tttgcacgtt gccaggtcac
    11581 gcggaaccat ttctagtgag caggtcgagc agatatctcg cgacctggcc gttgtcccag
    11641 aggctatgcg gaaggcactg gcgaccgacc aggaggcttc tcgggtcgct tactctgtgg
    11701 caaacgtggc tcacctcctc ttccttggca ggggcgtcgg ctaccccact gccatggaag
    11761 gcgctctgaa gctgaaggag atcagctaca tccacgccga ggggtacccg gcaggcgagc
    11821 tcaagcatgg gcccatcgcg ctgctggacc catcgatgcc gatcctggcc gtggccaccg
    11881 agtcccggac ctacgagaag ttggtgagca a
//
LOCUS       DATALS010000173         4447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_16780,
            whole genome shotgun sequence.
ACCESSION   DATALS010000173 DATALS010000000
VERSION     DATALS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16780"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VHS06_03790"
     CDS             complement(<1..254)
                     /locus_tag="VHS06_03790"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX2987274.1"
                     /translation="MKERDEAQLVATASAGDQAAFTELVRRYRDRVYGYCYHRTGNFE
                     DARDLAQESFVRAYTSLGQLRDTSGFGPWVRRIAANLCNR"
     gene            361..1170
                     /locus_tag="VHS06_03795"
     CDS             361..1170
                     /locus_tag="VHS06_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019507212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2987275.1"
                     /translation="MRTLQLPSGRQMPVLGQGTWRMGADRAQRQAEIEALRLGIELGM
                     TMIDTAEAYASGGAEEVVAEAIAGRRDEVFLVSKVSPDNATRQGTITHCEASLRRLKT
                     DHLDIYLLHWPSAIPFAETLEAFRQLKESGKIRDYGVSNFDVRQMEEARALPCGDIIA
                     TNQVLYNLVEREVERDILPWCLEHGVPIQAYTPIGRGRMLAHPALKEIASRHNATPAQ
                     IALAWLLQQPNVAAIPKASNPEHVRENRAALDIKLTEEDLAEIDRAFPPQS"
     gene            1227..1520
                     /locus_tag="VHS06_03800"
     CDS             1227..1520
                     /locus_tag="VHS06_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX2987276.1"
                     /translation="MRTAAIAKLKAGLSGYLESVKAGEQIVVTDRGRPVALIVPIDPN
                     ISEDERRAELVAKGLVRPGTGPIPTELIEGATVPGLTPDRALQAIMEDREDRV"
     gene            1517..1939
                     /locus_tag="VHS06_03805"
     CDS             1517..1939
                     /locus_tag="VHS06_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2987277.1"
                     /translation="MKFWDSSALFVLLTIQQGSSSAAAVFGSDPEVAMWWGTPVELAS
                     AAARLRRMGEVDDTGYARILSKIGPLAQHAEEVQPTEEVRQTALRLIRVHDLRAADSL
                     QLAAALVWARHVPTGLGFVCLDARLRAAAEKEGFSVLG"
     gene            complement(1923..2486)
                     /locus_tag="VHS06_03810"
     CDS             complement(1923..2486)
                     /locus_tag="VHS06_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987278.1"
                     /translation="MDRLSDELQQEADAPLAESGLIQLLEKHGNVTIGGSYKYQLMTV
                     SDIDLYVTGTDAGRDQGKRIVCELIDQGFWRGYALEDFVQFPREDFPTGIYIGLKRSF
                     RERFWKVDIWNLPEIPKDVLCLDEAMSQLSDARRIAIMKIKRWRDSNSLKIPSKSIYD
                     AVLLGRACDVESFKRFEACDGFLSPER"
     gene            complement(2573..>4447)
                     /locus_tag="VHS06_03815"
     CDS             complement(2573..>4447)
                     /locus_tag="VHS06_03815"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEX2987279.1"
                     /translation="MERRYVVPLFQRPYVWNRPRQWEPLWADITSKADRVLSYGDSRD
                     QHIRKHFLGAVVISQIRTFGRQVSAVDVIDGQQRLTTLQIILCALRDFATLNGFDETL
                     TELKRATENTCRREKEFETYKVWPTTSDRPVYESVLTAKSPEEVRKRHPLTWAKYARK
                     PSPLPLLVDAYLFFYESIESYVNDGAEIAEEGSDPPVLPTPEKETRLDSLIDAITDYM
                     ELVIIELEERDDPQVIFETLNARGEPLLPSDLLRNFVFLEATRAGEDVDRLYHTYWSA
                     FDDPSDQFWKAQQTIGRFKRSRMDVFVFHYLVSKTGLDISINHLYQEFRNWWLSAQTV
                     VENGLKGIKDASSTYRRLVQPPESDRLALFCARLALMDTGTVHPVLLFLTDAGVGCVP
                     EGALAGIVETLESYLVRRMICRMTPKNYNRVFLTLLRSMRDRMERGETIDRAFVRRAL
                     SEPQGDSVRWPSDSEFRDAWLTRPAYTDLQSSRATMILRALDLQMETSKQEELHLPGA
                     LSVEHVMPQNGSEEDWPIRLREEDGGDPTRAMAERTRVIHTLGNLTLLTQPLNSSVSN
                     GPFSAKRPEIAKQSRLRLNAYFQDFTNDDSWSEDSIWERGQKLFEVAKQVWPGPDEPV
                     "
BASE COUNT          939 a         1289 c         1278 g          941 t
ORIGIN      
        1 caccggttgc acaggttggc ggcgatccgg cggacccacg gtccgaaacc ggaggtgtcc
       61 ctgagctgcc ccaggctggt gtacgcccgg acgaaactct cctgggccag atcgcgtgcg
      121 tcctcgaagt tgccggtgcg atggtagcag tagccataga ccctgtcccg gtaccgccgc
      181 accaactccg taaaggccgc ctgatccccc gcagatgccg ttgcaaccag ttgtgcctca
      241 tcgcgctctt tcatcttcgc ctcctatgat aactgataca cgggcggggc aaaagatcac
      301 gcgaaggaga cagggtatca tctattacag caggattgtt ttcacaggag aggccggatg
      361 atgagaacgc tgcaactgcc gtcgggaaga cagatgcccg tgctgggaca aggcacgtgg
      421 aggatgggtg cggaccgcgc ccagcgacag gctgagatag aggcgctcag gctcgggata
      481 gagcttggga tgacgatgat cgataccgcc gaagcctatg caagcggagg tgcggaggag
      541 gtggtggccg aagcgatcgc ggggcgccgg gatgaggtct ttctggtgag caaggtctca
      601 ccggataacg caacgcgcca ggggacgatc acgcactgcg aggccagcct gcggcggctg
      661 aagacggacc atctggacat atacctgctg cactggccga gtgcgatacc atttgccgaa
      721 acgctggaag ccttccggca gctcaaagag tccgggaaga tccgcgacta tggggtcagc
      781 aacttcgacg tgaggcagat ggaggaagca agagctcttc cctgcggcga tatcatcgcc
      841 acaaaccagg tgctgtacaa ccttgtcgaa cgcgaggtcg agcgggacat cctcccctgg
      901 tgccttgagc acggcgttcc tatccaggcc tacacaccaa tcgggcgcgg acgcatgctc
      961 gcacaccctg cgcttaagga gattgcctca aggcacaacg cgacacctgc ccagatcgcc
     1021 cttgcctggc tgcttcagca gccgaacgtt gccgccatcc ccaaggccag caaccccgag
     1081 cacgtgcggg aaaaccgtgc ggctctggac atcaagctta cggaggagga tctggcagag
     1141 atagatagag cgtttccgcc gcagtcttga taaccgggga ggttatgact ataatagtca
     1201 tatagatagt gtaggtggta catattatgc ggaccgcagc gatagcgaaa ctgaaggcag
     1261 ggctttccgg ctaccttgag agcgtgaagg cgggggaaca gatcgtggtg actgaccgcg
     1321 gccgcccggt agcgctgata gtgcctattg acccgaatat atctgaggat gagagaaggg
     1381 cggagcttgt cgcgaagggg ctcgtaaggc ccggcaccgg accgatccca acagagctga
     1441 tagaaggagc caccgttccc ggcctgaccc ctgatcgtgc attgcaggcc atcatggaag
     1501 accgcgagga cagagtatga agttctggga cagctctgcg ctctttgtcc tcctgacaat
     1561 ccagcagggc agttcaagcg ccgctgcagt gtttggctct gatcccgaag tagcgatgtg
     1621 gtggggcacg cctgtcgagt tagcgtcggc agcggcccga ctgcgcagga tgggtgaagt
     1681 ggacgacacc ggctacgcgc gcatactctc caagatagga cctctcgctc agcacgctga
     1741 ggaagtccag ccgactgagg aagtcagaca gaccgctctc aggctgatcc gggtccacga
     1801 ccttcgcgcc gctgactcgc tccaactcgc cgcggctctt gtctgggcca ggcacgttcc
     1861 gacgggtctc gggttcgtat gtttggacgc gaggctgcgt gcagccgctg agaaggaggg
     1921 cttcagcgtt ctgggctgag gaagccatcg cacgcttcaa agcgtttgaa cgactccaca
     1981 tcgcaagccc ttcccagcaa cacggcgtcg tagatggact tggaggggat cttcagcgag
     2041 ttggagtccc tccagcgctt gatcttcatg atggctatcc ttcgcgcatc gctaagctga
     2101 gacatagctt cgtcgagaca cagcacatcc tttgggatct ccggcaggtt ccagatatcg
     2161 accttccaga acctctcccg gaaactccgc ttcagaccga tgtagatgcc cgtcgggaag
     2221 tcttcacgcg ggaactgcac gaagtcctca agcgcatacc ccctccagaa cccctggtcg
     2281 atgagctcgc agacgatcct cttgccctga tcacgcccgg catccgtgcc ggtgacgtat
     2341 aggtctatgt cggagacggt catcagctgg tacttgtagc tgcccccgat tgtgacgttt
     2401 ccgtgctttt caagaagctg gatcagcccc gactcggcca gcggggcatc agcttcttgt
     2461 tgtagttcat cggagagacg gtccatatcc tcctcatagc cagagcactc actggttcta
     2521 cttcgttgtc actgctctcg ccacttccag caactgctgc ttgacgcgca tctcagacag
     2581 gctcgtccgg tccgggccat acctgcttcg ctacctcaaa gagcttctgc ccccgctccc
     2641 aaatagagtc ctcagaccac gaatcgtcgt tcgtgaagtc ctgaaaatag gcattgagac
     2701 gaagacggct ctgttttgct atctctggac gcttcgccga gaagggaccg ttgcttactg
     2761 atgagttgag tggctgggta aggagagtaa ggttccccaa agtatgtatg acgcgagttc
     2821 tttctgccat cgctctggtg gggtcgccac catcctcctc ccttagcctt atgggccagt
     2881 cttcctcact tccgttctgg ggcatcacgt gctcaacaga aagcgctccc ggaaggtgta
     2941 actcctcttg tttggaggtc tccatctgga ggtcaagcgc cctaaggatc atcgttgccc
     3001 tgctcgactg aagatcagtg tacgccgggc gcgtgagcca tgcgtcgcgg aactcgctgt
     3061 cactcggcca gcgtacgcta tccccctggg gttccgagag tgcgcgcctt acaaaggctc
     3121 tatctatggt ctctcctctt tccattcggt cgcgcatgga cctaagcaat gttaggaaca
     3181 ccctgttgta gttctttggc gtcatcctac agatcatacg cctaactaag tatgactcca
     3241 aggtctcgac aatgcccgcc aacgcgcctt cggggacgca ccccacgcca gcgtcagtga
     3301 ggaacagcaa gacagggtgg actgtgcctg tatccattag agcaaggcgc gcacagaaga
     3361 gcgccagtct gtcggactct ggtggctgga cgagccttcg ataggtcgag ctagcatcct
     3421 tgataccctt cagcccattc tccacgaccg tttgagcgga tagccaccag ttccggaact
     3481 cctggtacag gtggttgatg gagatgtcca agcccgtctt gctcacaagg taatggaaga
     3541 caaacacgtc catgcgagag cgtttgaacc tgccgatggt ctgctgggcc ttccagaact
     3601 ggtcagacgg atcatcaaaa gcagaccagt acgtatggta gagcctatcg acatcttctc
     3661 cagcccttgt ggcttctagg aagacgaagt tccgcagcag gtctgaggga agcaatggct
     3721 cacctcgagc attcagcgtt tcaaagataa cctgaggatc atccctctct tcgagctcaa
     3781 tgataacgag ttccatataa tcggtgatgg cgtcaatgag tgagtctagt cgcgtctctt
     3841 tctcgggagt tggtaggaca ggtgggtcgg agccctcttc ggcgatctct gctccatcgt
     3901 tcacatagga ctcgatactc tcgtagaaga aaaggtacgc gtctacgagg agcggcaatg
     3961 ggctcggctt cctagcgtac ttcgcccacg ttagtggatg cctcttccgt acctcttctg
     4021 gcgattttgc cgtaagaact gactcgtata ctggccgatc actcgtggtc ggccagacct
     4081 tgtacgtttc gaactccttc tcacgtctgc atgtgttctc tgtggctctt ttcagttccg
     4141 taagcgtctc atcaaagcca ttcagggtgg cgaaatccct aagggcacag agaattatct
     4201 gcagagtagt gagacgctgc tggccatcta tgacgtccac ggcggagacc tgcctgccga
     4261 aggtacgtat ctggctaata actactgccc ccaaaaagtg cttccgaata tgctgatccc
     4321 tgctgtcacc ataactcaga actcggtcag ccttgctggt gatatccgcc cagagtggct
     4381 cccactgacg cgggcggttc cagacatacg gacgctggaa gagcggcact acgtatctgc
     4441 gttccat
//
LOCUS       DATALS010000174         6448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_374206, whole genome shotgun sequence.
ACCESSION   DATALS010000174 DATALS010000000
VERSION     DATALS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374206"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="VHS06_03820"
     CDS             <1..1473
                     /locus_tag="VHS06_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2987280.1"
                     /translation="KGVLFGLGFSAQDLDKPVEVLSGGQKVRLGLTKLLLTEPALMLL
                     DEPTNHLDLAATEWLETYLGGLKSAALVISHDRYFLDRVTTKTLEVEERTGVVYPGNY
                     SYSREAKQLRLEQAVEAFERQQKERTKLREFYEKWRATPTHKNQAMSRKRQLDKMEVL
                     DRPVTHHKSMKLGLSINHRSGNDVLELDGLGMAFGAHQLFSGVNMHVSMGDRVALVGP
                     NGAGKTTLLKILLGHLEPTAGHLRWGAGVELGYFSQELEGLDPSRTCLEEMLAIPGFT
                     RFDAHALLGRFLFSGEDALKSIAQCSGGERNRLILAKLTVSGANVLLLDEPTNHLDLE
                     SKTILEETLREYPGTLLFVSHDRFFVDQVATKVWEFESGRVTAYDGNYTAYKAEKERL
                     AALEAAEAPLPPKPSSAAREQAKEAERTKRREERLAEEAVRQVEEGIARFEARKGELE
                     ALLGDPEVYKTPAGGEAAAEYRTVLEELERLYAEWEKVYR"
     gene            complement(1798..2577)
                     /locus_tag="VHS06_03825"
     CDS             complement(1798..2577)
                     /locus_tag="VHS06_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987281.1"
                     /translation="MRFFGEAWKLLKRSWRRIVKFALFMMMIRVLVLGLEFGIGLVFR
                     YFSKRWCSPTAFVLGFTSFYWLVGLLGVLPAEKYMWFSVVSSAYRTEEPKLWQLYFQS
                     FRSWTRMIMGGLWWGVFASPLMVVGALLFLPGLPGGGGHWREHLASFAWRSMPLIPFL
                     GALSVYGSFAVLGIICEGRPALVSWRRAFSLIPRRELLATVGLLSALYSSVVLFGLLL
                     WQILRVPAGLSEWVTFAMLAGLSISHFTLWHAHTRAEGAQN"
     gene            complement(3018..3863)
                     /locus_tag="VHS06_03830"
     CDS             complement(3018..3863)
                     /locus_tag="VHS06_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987282.1"
                     /translation="MRRLLALLVAFLTSLSQGALAIGTLEQLQLRGPVKRCFFRFSTI
                     GQPDTHGWSACRDFDEQGRMTYYCTFNPDGSVEAETRWRYILDGDRLVGIVRTGEDPS
                     ESFVRYDDAGRVVVTETRTPENRRMLIYLDAEGHQVGTESVSPDGSVQTRSVTTHSSD
                     GKPAKTDEFDASGRSVSTTESTSTPEGRPLTRKTSPIPGSQIVGSFVLVTHEYDERGL
                     MVSRSMIESEGVLPVSRWRYEYDDFDDYGNYTTVTTYERNEWQREISDFQLTDTEKRR
                     ITYYD"
     gene            complement(3966..4130)
                     /locus_tag="VHS06_03835"
     CDS             complement(3966..4130)
                     /locus_tag="VHS06_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987283.1"
                     /translation="MTGISLWTGFLIVLFVLAAVGGLSKTDKAIKAKLNVIRLPFGGK
                     DDDGKSDKRV"
     gene            complement(4719..4991)
                     /locus_tag="VHS06_03840"
     CDS             complement(4719..4991)
                     /locus_tag="VHS06_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987284.1"
                     /translation="MTRKNESLRQMSPRQKFLFMLKISAITMVGEFIFAMVALRPNYP
                     DSKWYEYVPFILAFWPLFPLYFYGTGAIGKLARIAVDNIRGLIRRD"
     gene            complement(5183..5446)
                     /locus_tag="VHS06_03845"
     CDS             complement(5183..5446)
                     /locus_tag="VHS06_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987285.1"
                     /translation="MSALIKNDGILDEGHLVPWSDIKDLRLLHYRGRLEKVLARIHDR
                     TLTVEPKDVSVDLAQVAQMAHGPRVRRVYVETTWPNYLLLVAV"
     gene            complement(5546..6286)
                     /locus_tag="VHS06_03850"
     CDS             complement(5546..6286)
                     /locus_tag="VHS06_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987286.1"
                     /translation="MARITPEGVSHISKTIAWAEIDEVKIMVNELGGNTTDVWVKSKG
                     FTGTIHPSMQDVERAKALITQNVASERISYDEFTPSYLALKRVRDVFIKVVWVALVGL
                     VAYYILRYRPSATGLFKSATDTATSLGPLSVLVTLLGVHQLMFIKECWAPMYRIWRFR
                     YLRTDPPERVAFDHGVILTVAGASALLLALAAGEWHGLALGIVGLALLVAVKRCSSDG
                     LRGWLAVLTLILLSVAILVQRATDAPFF"
BASE COUNT         1428 a         1936 c         1865 g         1219 t
ORIGIN      
        1 aagggggtgc tcttcggcct cggcttctcc gctcaggatc tcgacaaacc cgtcgaggtc
       61 ctctcgggtg gccagaaagt ccggttgggc ctgacgaaac tcctcctcac ggaaccggcg
      121 ctcatgctcc tcgatgagcc caccaaccac ctcgacctgg cggccaccga gtggctcgag
      181 acctacctcg gcggcttgaa atcggctgcc ctggtcatct cccacgaccg ctacttcctc
      241 gaccgggtga ccacgaagac cctggaagtc gaggagagga cgggcgtcgt ctacccgggg
      301 aactacagct acagccggga ggcgaagcag ctccggctgg aacaggcggt cgaggccttc
      361 gagcggcagc agaaggagcg gaccaagctc cgagagttct atgagaagtg gcgggccacc
      421 ccgacgcaca agaaccaggc catgagccgc aagcgccagc tggacaagat ggaggtcctg
      481 gaccgcccgg tgacccacca caaatcgatg aagctgggcc tctcgatcaa ccatcgctcc
      541 ggcaacgacg tcctggagtt ggacgggctg gggatggcct tcggggccca tcagttgttc
      601 tccggcgtga acatgcacgt ctccatgggt gatcgggtgg ctctcgtcgg tcccaatggc
      661 gccgggaaga ccaccctgct caagatcctg ctggggcacc tcgaacccac cgccggccac
      721 ctgcgctggg gcgccggggt cgagctgggc tacttcagcc aggagctgga gggactggac
      781 cccagccgga cctgccttga ggagatgctg gccatcccgg gtttcacccg gttcgacgct
      841 cacgccctcc tcgggcggtt cctcttctct ggcgaggacg ccttgaagtc catcgcccag
      901 tgctcgggcg gcgagcggaa ccggctgatc ctggcgaagt tgaccgtctc cggcgccaat
      961 gtgctgctgc tcgatgaacc caccaaccac ctggatctcg aatccaagac gatcctcgaa
     1021 gagacgctgc gggagtaccc cggaaccctg ctcttcgtct cccacgaccg gttcttcgtg
     1081 gaccaggtgg cgacgaaggt ctgggaattc gagtcggggc gggtcaccgc ctacgacgga
     1141 aactacaccg cctacaaggc tgagaaggag cggctggcgg cgttggaggc cgccgaggct
     1201 ccattgccgc cgaagccctc gtctgccgca cgtgagcagg cgaaagaggc cgagcggaca
     1261 aaaaggcgtg aggagcggtt agccgaggag gccgtgcgcc aggtggagga ggggatcgcc
     1321 cggttcgagg cccggaaggg agaactcgag gcgctgctgg gcgacccgga ggtgtacaag
     1381 accccggcgg gcggcgaggc ggctgccgag taccgcacgg tgctagagga gctggagcgc
     1441 ctatacgccg aatgggagaa agtctaccgg tagagcaagg tcagggagtg agtgccatta
     1501 gctggccccg ccgttggacg gcttagcggc cagccgatgg gcactcactc cctaaccctt
     1561 ggactacgag agcagcacca accgtcaaac acgaacaccc aatccccaat ccctatccca
     1621 cccggtagac atgttcccgc agacgagtca cactgaggcg gtggcggtgg tgacgaagga
     1681 gtaagattgg gaattgagct gtaaggaacc cgggggcaca aaagaccgcc cgagaatggg
     1741 cggcgatgtg tcgatgacag ggtctgtaag cgtcataagg gtcttaggac ggccccgcta
     1801 gttctgcgcc ccctcggccc gcgtgtgggc gtgccaaagg gtgaagtgag aaatcgagag
     1861 gccggctagc atagcgaagg tcacccactc acttagtcca gcgggaaccc gcaggatttg
     1921 ccacagcaac agcccaaaga ggaccactga ggaatagaga gccgagagca acccgactgt
     1981 agccagcagt tcacggcgtg ggataagcga gaacgcccgt cgccatgaga cgagagcagg
     2041 ccttccttcg caaatgatgc ctagcacagc gaaacttcca tagacagaga gggctcccag
     2101 gaatggaatg agcggcatcg atcgccatgc gaatgaagcc aggtgttcgc gccagtggcc
     2161 gccgcccccg ggcagcccag gcagaaacag gagcgccccc accaccatga gcgggctggc
     2221 aaagactccc caccacaagc cccccataat catcctcgtc cacgagcgga acgactggaa
     2281 atacagctgc cagagcttgg gttcttccgt acggtaggca cttgagacga cggagaacca
     2341 catgtacttt tcggccggaa gcacccctag cagccctacc aaccagtaga agctggtgaa
     2401 gccgagtacg aaagccgttg ggctgcacca tctctttgag aaatagcgga agaccagtcc
     2461 gatcccgaac tccaggccga ggactagtac gcggatcatc atcataaaca gcgcaaactt
     2521 gactatccgc cgccacgacc ttttgagaag cttccacgcc tcgccaaaga atcgcatttc
     2581 gcatctagcc ccccaacgcc tggactctcg acggatcact ggagagccaa cttagtcctc
     2641 cattctccca gcccttccag gcgaggtgtc gcgggcgccc ggttcaggcg cccgcaacac
     2701 ctcaccatag cgacgtccgc ttgggtttct tcacttcggt ttcctggaag tccctatccc
     2761 ggtcagtcac gttcgactgg gaatctcgat cagactccga ttggtcacac tctccaccgc
     2821 cttacttttt cgtcatcggg tccaagcgcg agatccgtcg actttgggcc caggcgtgtg
     2881 gctgcggtgt tgatagacga aggcggcagg gggctggttt gtccccaccc caacccgtct
     2941 gatcgacatg ctcccgcaga cgcgtcaggc agaatacgga tgcttgacaa ggtcgtcaac
     3001 cgacggtata tggcggttca gtcgtagtag gtaatgcggc gcttctccgt gtccgtgagt
     3061 tggaaatcgg agatttctct ttgccactcg ttgcgctcat aggtcgtcac ggttgtatag
     3121 ttgccgtagt cgtcgaagtc gtcgtactca tacctccaac gactgacggg cagcacaccc
     3181 tccgattcta tcatcgacct tgagaccatg aggccccttt catcgtactc gtgcgtgacc
     3241 aggacgaatg accccacgat ctggctaccc ggtatcgggg aagtcttgcg tgtcagaggc
     3301 ctgccctctg gtgtgctcgt ggactcggtg gtggaaacag accttcccga ggcatcgaac
     3361 tcgtcggtct tggctggctt tccatccgac gagtgggtag tgaccgagcg tgtttggacg
     3421 gacccgtccg gtgagacgga ctccgtaccg acctggtggc cttcagcatc gaggtagatc
     3481 agcatcctcc gattttcagg agtcctagtc tcggtaacca ccactctccc ggcgtcgtcg
     3541 tatcgaacga acgactccga ggggtcctct ccggtccgca cgatgccgac aagtcggtcg
     3601 ccgtcgagga tgtagcgcca ccttgtctcg gcctcgaccg atccgtccgg gttgaacgtg
     3661 cagtagtaag tcatccggcc ctgttcgtcg aagtctcgac aagcggacca tccatgagta
     3721 tccggctgcc caatcgtcga gaacctgaag aagcatctct tgaccggccc tcgcagttgg
     3781 agttgttcca gcgtcccgat ggccagcgca ccttgggaca ggctggtcaa gaaagcaaca
     3841 agcaacgcca gaagccggcg cattcggcac cctccttcca cccttgccag agttccccac
     3901 ccaggcagat tcgtagtgca actgtacatt ccgttcatac ggaattagaa tgcttgacag
     3961 ggttgtcaaa ctctcttatc ggactttccg tcgtcgtcct tcccgccgaa gggcagtcgg
     4021 atgacgttca gttttgcctt gatcgccttg tccgtcttgc taagccctcc gacggctgcc
     4081 aaaacgaaga gcacgatgag gaatcccgtc cacaacgaaa ttccggtcaa caggctgctc
     4141 acctccaacc aaatcccgag gggacgttcc gcaggagtgt ctcttctcct cttgcatcga
     4201 cggcatggct aatagtgcaa ctgtagattc ggttcacacg aaataagggt gcttgacaag
     4261 attgtcatgc gccgttagga acgggggcag gaaacccggg tccgggccac accaactccg
     4321 ggttggcgag aggtggttga ggtattccgt tatgagcgga actgtttcgt cgtccgtgta
     4381 caacatggac atgatgtcct cttcaagcct attcacggct gcctccgaga aagccgcagg
     4441 atatgacagc atctcggttg tacacttcgg caggccgcct gccacatcct ggaaggacat
     4501 gtgatgagat ttgtagtgca actgtacatt ccgttcacat agaagcggtg gcggtggtga
     4561 cgcgcgggta ggcatggaga gaaaagggga aagagaagac cgcccgagat cggcggtcta
     4621 tgcgttgccg gtgggtcaac gggttgatgt atcctggcgg ttccgaacga ggggcacccc
     4681 gggacatctg gacagcgctt tcgtggcccc gccgagaatc agtctctacg aataagccct
     4741 cgaatattgt ccaccgcaat cctagccagt ttcccaatcg caccagtacc gtagaagtac
     4801 agtgggaaga gtggccagaa agccagaatg aatggtacgt actcatacca cttggagtct
     4861 gggtagttgg ggcgaagcgc gaccatcgcg aaaatgaact caccgaccat cgttattgca
     4921 gagattttca gcatgaagag gaacttctgc ctcgggctca tttgcctcag ggactcgttc
     4981 ttgcgggtca cgaccacata cctcgattcg actatctgag tcgcgatagt agaaagtacg
     5041 caccagacgt aataatggca atcccccacg ctagatcgga taccctgaat ccaacaggtc
     5101 tccgaaccag gacgatgaga agaagagcca gcaagatggg gatgaacagc cagtccagtc
     5161 gatggtagcg caggatcgat aatcagacag ccaccaacag gaggtagttg ggccatgtcg
     5221 tctctacgta gacccttctc acgcgggggc catgtgccat ctgagccact tgtgccaagt
     5281 cgaccgatac gtccttgggt tcaacagtca atgtacggtc atgaattcgt gcgaggacct
     5341 tttcaagcct accccgataa tgaagcaagc gaagatcctt gatgtcggac cagggaacta
     5401 gatgtccctc gtccagaatg ccgtcgttct taatcagtgc gctcatttgc cctctccctt
     5461 tctaaaaacc agggaagcca agacgaaaac ctcgaattcc ccctgagata aggagaaaga
     5521 gaagaccgcc gagattgcag cgggttcaaa agaaaggtgc atccgttgct cgctggacca
     5581 gaatggccac ggacagcaga attagcgtca gaaccgccaa ccagccacga agtccatccg
     5641 aggagcacct cttgaccgcc acgagcaaag ccaaaccaac gattccgagc gccaatccat
     5701 gccactcacc tgccgccaac gccaacagaa gggccgaagc tcccgcaacg gtcaggatga
     5761 caccgtggtc gaaggcaacc ctctcgggag gatccgtccg caggtagcgg aagcgccata
     5821 ttcggtacat gggtgcccag cactccttga tgaacatcag ttgatggact cccagcagtg
     5881 tcacgagtac cgaaagcggg ccgagagatg ttgctgtgtc cgtcgcactc ttgaataggc
     5941 ccgtcgcaga agggcggtag cgaaggatgt agtaggccac caatcccacc aacgccaccc
     6001 agacgacctt gatgaacacg tcccgtactc gcttcaacgc caggtacgaa ggcgtgaatt
     6061 cgtcgtagga gattctttcg gaagccacgt tctgggtaat gagcgccttc gctctctcca
     6121 cgtcctgcat actcggatgg attgtgccgg tgaagccctt gctcttcacc catacgtcgg
     6181 tcgtgttacc gcccagctcg ttgaccatga tcttcacttc atcgatttcg gcccacgcaa
     6241 tggtcttact gatgtgcgaa acaccttcgg gcgtgatgcg tgccatagac atcacctctc
     6301 tcgctcgaaa tcgtgctcat cggcaccgtt cacttaaggt agaggatgtc gtagcaactc
     6361 atggtggcga tgttgttcga actgaaacca tatctccgtt tcggcgcagg gcctattcaa
     6421 gagtgcgacg gacacagcaa catctctc
//
LOCUS       DATALS010000175         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_278186, whole genome shotgun sequence.
ACCESSION   DATALS010000175 DATALS010000000
VERSION     DATALS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278186"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /gene="ispD"
                     /locus_tag="VHS06_03855"
     CDS             <1..624
                     /gene="ispD"
                     /locus_tag="VHS06_03855"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX2987287.1"
                     /translation="DKVWLPLGGVPVVAYSVKAFAACNPQPRVALVVSRERLSGARAL
                     VGELGIDAVVCVGGERRQDSVQNGLAALGNVSVVAIHDGARPLVSPELIERCYAEAAN
                     HGAAVPGVPVRDTLKRVSDDGWVQATVDRGGLWGVQTPQVFRMELLLEAYAALRGEVT
                     DDAAVVEGLGRPIKMVPGDPRNLKLTTREDLIVARALLGLPATEGNG"
     gene            626..1132
                     /gene="ispF"
                     /locus_tag="VHS06_03860"
     CDS             626..1132
                     /gene="ispF"
                     /locus_tag="VHS06_03860"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX2987288.1"
                     /translation="MGVGFRVGIGYDAHRLVEGRACILGGVEIPHSRGLEGYSDADAL
                     LHAIMDAMLGAAGLPDIGKHFPPGDERFHNISSLRLLAVVRGMVEERGWRVGNVDSTL
                     MAEEPRVGPHVAAMKDAISSTLGIASDDVGIKATTNEGMGFVGRGEGIAAMAVVLLSR
                     AAAEDLEE"
     gene            1104..2225
                     /gene="cysS"
                     /locus_tag="VHS06_03865"
     CDS             1104..2225
                     /gene="cysS"
                     /locus_tag="VHS06_03865"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX2987289.1"
                     /translation="MPPPRIWRNRLGLRVFNTLTGSEEEFKPLGDVVEMYVCGMTPKF
                     HPHVGHARIFVAMDVIRRYLEYRGFKVHHVQNFTDVDDKIIARSNKEGIPAHEVAQKY
                     TNSYFEVMDALNVEHAHDYPKATHVIPSIVAFIEKLMAEGFAYEVEGNVYFQVARFPE
                     YGRLSGRTEEEGVMAGARVEVEPGKRDPRDFALWKRAKPGEPAWDSPWGPGRPGWHIE
                     CSTMINETLGPQIDIHAGGRDLIFPHHENEIAQSEAFCHCSPFVRYWVHIGLLNIGGE
                     KMSHSLNNFLTIQQLLEKYEPVVVRLYLISIPYRAPMTYSEEGLDAAAKGLARMRSAL
                     SASSAPSAQRAARPEEVEAAKKLEEAVEKSFQDALFPSC"
     gene            complement(2206..2328)
                     /locus_tag="VHS06_03870"
                     /pseudo
     CDS             complement(2206..2328)
                     /locus_tag="VHS06_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
BASE COUNT          437 a          625 c          829 g          437 t
ORIGIN      
        1 gacaaggtat ggcttccact gggaggggtg ccggtggtag cctattctgt taaggcattt
       61 gcagcgtgca acccgcagcc tcgggttgcc ttggttgtga gccgggagag actatcgggg
      121 gcgagggcgc tggtagggga gttggggatc gatgccgtcg tctgcgtcgg gggtgagcga
      181 cggcaggact cggtgcagaa cggcctggcc gcgctcggga acgtctcggt cgtagccatt
      241 cacgacggcg cgcggccgct ggtcagccct gagctgatag agcgatgcta tgctgaggcg
      301 gcgaatcacg gcgcggcagt accgggtgta ccggttcggg atacgctgaa gcgggtctcc
      361 gacgacggtt gggtacaggc taccgtggac cgcggcggac tgtggggcgt tcagacgcct
      421 caggtctttc ggatggaact actgctggag gcgtatgccg cgctccgcgg agaggtgacc
      481 gacgacgccg ccgttgtgga ggggctggga cgtccgatca agatggtgcc cggcgatccc
      541 cggaacctca agctgacgac gagggaggac ctgatcgtgg cgagggcgct gctggggctg
      601 cccgcgaccg aggggaacgg ctagagtggg agttggattt cgagtgggca tcgggtacga
      661 tgcccatcgt cttgtggaag ggcgggcttg tatcctcggg ggggtggaaa tcccgcactc
      721 gagagggctg gaggggtact ccgacgcgga tgcgcttctg cacgctatca tggatgcgat
      781 gctgggcgcc gccggattgc cggatatcgg aaagcacttt cccccggggg atgagcgctt
      841 tcacaacata tccagcctga gactgcttgc ggtggtcagg gggatggttg aagaacgagg
      901 ctggcgtgtg ggcaacgtgg attccacgct gatggctgag gagcctcgag ttggtccgca
      961 cgtggccgcc atgaaggatg cgatctcctc gacgctgggg atagccagcg acgatgtggg
     1021 gataaaggcc acaaccaacg agggcatggg ttttgtcggt cggggtgagg ggatcgccgc
     1081 gatggccgtg gtgctgcttt cgcgtgccgc cgccgaggat ctggaggaat agattgggcc
     1141 taagagtttt caatacgctg accgggtcgg aagaggagtt caagccgcta ggcgatgtcg
     1201 tagagatgta cgtgtgcggg atgacaccga agtttcatcc ccacgtcggc cacgcgagga
     1261 ttttcgtggc gatggacgtg atccgtcgtt acctggagta ccgtgggttc aaggtgcacc
     1321 acgtccaaaa ctttaccgac gtggacgaca agatcatcgc gcgctccaac aaagagggga
     1381 tcccagcgca tgaggtggcg cagaagtaca ccaactccta cttcgaggtt atggacgcgc
     1441 tgaacgtcga gcacgcccac gactacccga aggctacgca tgtgataccg tccatcgtcg
     1501 ccttcatcga gaagttgatg gcggaagggt tcgcctatga ggtggagggg aacgtctact
     1561 tccaggtggc tcgcttcccg gaatatggaa ggctctcagg tcggaccgaa gaagagggcg
     1621 tgatggcggg tgccagggtc gaggtggagc ctgggaagag ggatccccgg gacttcgcac
     1681 tgtggaagag ggcgaagccg ggagagcctg cctgggacag cccgtgggga cccgggcgtc
     1741 ccggttggca catcgagtgc tccacgatga ttaacgaaac gctggggccg cagatcgaca
     1801 tccatgccgg tggccgtgat ctgatattcc cccaccacga gaatgagatc gcacagagcg
     1861 aggcgttctg ccactgctcg cccttcgtcc gatactgggt gcatataggg ctgctgaaca
     1921 tcggcggcga gaagatgtct cactcgctga acaatttcct taccatacag cagctgctgg
     1981 agaagtacga gcccgtggtg gtgcgactct acctgatctc cattccctac cgagcgccaa
     2041 tgacctacag cgaggagggg ttggatgccg cggccaaggg gctggctcgg atgcgctctg
     2101 ccctgtcggc ttcgagcgca ccgtctgctc agcgtgcggc tcgacccgag gaggttgagg
     2161 ctgcgaagaa gctggaggaa gcagtcgaga agagcttcca ggatgcccta ttcccgtctt
     2221 gttaacctct tccaacaact ccgcctcgag cgccgcaatc ctcgggtccg ggttgggctc
     2281 ccccaggttc cgaagcagcg accgcttcgc caggtaggcg cacttctc
//
LOCUS       DATALS010000176         9748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_98204,
            whole genome shotgun sequence.
ACCESSION   DATALS010000176 DATALS010000000
VERSION     DATALS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9748
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98204"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VHS06_03875"
     CDS             <1..420
                     /locus_tag="VHS06_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX2987290.1"
                     /translation="AMKDRQVDGFAVTGGVPTSAITDVTTAIDISLLPITGDDAKKVM
                     DKYKFFVPVKVPATAYKGLAGETSTIAVQSMLVARDDLDPDLVYWLTKTLIEKQADLA
                     QAHAKGKELSKESAVKGLTVPLHPGAEKYYKEIGVIK"
     gene            505..1050
                     /locus_tag="VHS06_03880"
     CDS             505..1050
                     /locus_tag="VHS06_03880"
                     /inference="COORDINATES: protein motif:HMM:NF020479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HEX2987291.1"
                     /translation="MHLLHFKQYSAASRGRLFLLVPLALLALAALAAWALWPTPTLDI
                     RVGQEARLVRSIPVSTGEMITYTYLHSVQKKPIDEVMEIAPNGHLVVRETTYEMTGAG
                     LPSDVLDGDFIIDPATGKWKIINMSRDIPEWHVRVAFTAQQTLEVGGEKFRLDSLATP
                     TTVLIVGVANHPHIVALLGLG"
     gene            1047..3095
                     /locus_tag="VHS06_03885"
     CDS             1047..3095
                     /locus_tag="VHS06_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX2987292.1"
                     /translation="MNSSVVIIEEVVWRKKYPPQAFETVSEEEVERITAKLDVGSRLR
                     KFVGPFGIFIIIMAAAMSIFHLYTTFFGFFDAPIQRAAFFIFVFVMGFALYPARFKGT
                     LDTRRIPPWYDLILMGLSAMVGGYIIVNFENIVENMGNPTQLDLIMGSIACLLVLELC
                     RRAVGPVLMFICLFFILYAFWGNYIPGFLSHRGYPFTRVVSHMYLTTEGIFGVATGVA
                     TTYVFMFILFGAFLVRTGTGQFFNDIAVALLGGYSGGPAKVAVFASALEGTINGSSVA
                     NVVGSGSFTIPLMKSIGYKPYFAGAVEAAASTGGQIMPPVMGAGAFIMSEILGRPYIE
                     VCIAAALPAVLYFTGVLVGVHLEAKRTGLEGLPRNMLPSPMKVMKERGVMLLPIVAVV
                     WMLMNGYTPPYAAVTGIIASIVFGAMLKTTRVHPIKYWEALVAGARDALGVSIACISI
                     GFIMGSATLTGAGLKIANSIVMLSGGNLFLTLFFTMIASLILGMGLPTTANYLVTSTI
                     AAPAIVQAGVPLLSAHLFVFYFGIVADLTPPVALAAMAGAGLARAPPTKTGVQAFRLA
                     APAYLVPYVFCYAPQMTLLGYDFISGVEASVTSVLGVIALGVGLFGWMMTWVPWWQRV
                     PMLGAALLLITPGWETDLAGFIILAAVFMHQRYLVKSGKAKPVERTSILPGQTPGSPA
                     "
     gene            complement(3179..3943)
                     /gene="map"
                     /locus_tag="VHS06_03890"
     CDS             complement(3179..3943)
                     /gene="map"
                     /locus_tag="VHS06_03890"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX2987293.1"
                     /translation="MSESEAITLRSPKEIEKIREAAKIVVLTLKELKKAAQPGITTQE
                     LDDLAAKIFREHDAVSGSLNYYGYPGNICVSINEQVVHGIRGSRQLNEGDIVKFDVAA
                     SHKGYFADSTMTYPVGQVRPEVARLLKVTEEALFKGIDKAIVGNRISDISHAIQRHVE
                     KNGLSVVRAFVGHGVGRNMHEAPQVPHFGPPHQGPLLRPGMVLAIEPQVNMGSPEITV
                     LDDHWTAVTKDGKWSAHFEHTVAITNNGPDVLTRWE"
     gene            4207..4812
                     /gene="coaE"
                     /locus_tag="VHS06_03895"
     CDS             4207..4812
                     /gene="coaE"
                     /locus_tag="VHS06_03895"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871210.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX2987294.1"
                     /translation="MKHYLIGLTGNIGCGKSAVAAMLADLGAEVVDADLLVHQLMSPG
                     SESWQAIVDRFGRGILRPDDGIDRRALGEIVFRDPAALAYLEAILHPATRRLVEERIA
                     ASKSAVVVVEAIKLIESGWYRSLDALWVVACSREQQIERIMRTRGFKRAEAELRVDAQ
                     PPAEEKLRYADVIIDNSGSLETTRRQVADAWQRIPMVPDAG"
     gene            complement(4946..6022)
                     /locus_tag="VHS06_03900"
     CDS             complement(4946..6022)
                     /locus_tag="VHS06_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX2987295.1"
                     /translation="MTYTIPIGPYHPALEEPCKIEISCEGEVIKDAQLHVGFVFRGVE
                     RLAQQKNYIQNIALVERICGICSNVHTMTYCMAVERLAGVEPTERARYIRVVVAELER
                     LHSHILWAGIAAEVMGFQTLFMSCWQLREKVMDLLEAISGNRVNYAMNCIGGVNRDIT
                     DPDAVLNGVREIRREVERTMIPIFTGDRTIKARAAGVGVLTHEQAVALGTVGPTARAS
                     GVKQDIRKATPYAAYDRLEFQVPVQESGDVVARVVVRALEILESCSLVEQAVLLMPPG
                     PLRGPEFVQVPPGEAIARAEAPRGEVFYYVASDGSDIPTRVKARTPTYLNLTAARPMV
                     LGATLADVPLIQASIDPCYSCTDR"
     gene            complement(6019..6435)
                     /locus_tag="VHS06_03905"
     CDS             complement(6019..6435)
                     /locus_tag="VHS06_03905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX2987296.1"
                     /translation="MDVEMIRAQLGTAQPWEKRPDGYWLEDPLLKVRDMARCMVRNGA
                     RLMTVTATPVAGGEQRLAYHWDLEGQVTTLIAGTHQNTIDTIVDICPAADWIEREIHD
                     YFAVSFAGRQSTPPLTLRPNDPPGFFFWKGQDGEQS"
     gene            complement(6420..6932)
                     /locus_tag="VHS06_03910"
     CDS             complement(6420..6932)
                     /locus_tag="VHS06_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2987297.1"
                     /translation="MNILNVIVANLRQGPVTLRFPARVAPPSHYRGPVQIDTARCLAC
                     GVCAYVCPSAAITVTDRTDSCDWSYDPGRCTFCGRCADLCMGKALSMKEEPAPVYTTP
                     GALAETHQVHYPLCPQCGRPALPISDELLAKALSELTDEARSRSRLCDRCRKRHTQRT
                     LKETTRWTSR"
     gene            complement(6937..7332)
                     /locus_tag="VHS06_03915"
     CDS             complement(6937..7332)
                     /locus_tag="VHS06_03915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX2987298.1"
                     /translation="MPAILALLLCIPVKLRLNPFSIPNAEQEIYAGPTTEYDGRSLAI
                     WELAHGLEWVALTGLAITLGLPVHPTNWIVGVALFVVLSMLLVLILTTLAAATARLKV
                     TQASRVYWKWGMGIAVLALVMAALPGLRG"
     gene            complement(7293..7856)
                     /locus_tag="VHS06_03920"
     CDS             complement(7293..7856)
                     /locus_tag="VHS06_03920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX2987299.1"
                     /translation="MNLIQGLLALLIFPGLLYAVPVAWLMLWMERKLTARMQGRIGPP
                     FYQPFFDFVKLMAKRPITRDPLEGMLMTGLPVLAVGAALGSLALLPVIPGSTGFTGDM
                     VLLVALLELPAICSVLAGFASRSIFGQVGAVREAVLSISYNLPFLAALMALAAAAGSL
                     RLSDVALLTPPPWFACRRSWRCCSAYR"
     gene            complement(7853..8326)
                     /locus_tag="VHS06_03925"
     CDS             complement(7853..8326)
                     /locus_tag="VHS06_03925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX2987300.1"
                     /translation="MRQNGRCLMGLIDELVGSARRKSPWLFHLNTGGCNGCDIELVAC
                     LTPRYDVEQLGIRLEASPRHADVLCVSGPVTRNTRHALETVYAQMPDPKVVVALGTCP
                     ASCNVYAGSPAVEGPVNEVIPVDVFVPGCPPRPDAIIAGIAEAVKILVAKQEAQQ"
     gene            complement(8351..>9748)
                     /locus_tag="VHS06_03930"
     CDS             complement(8351..>9748)
                     /locus_tag="VHS06_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX2987301.1"
                     /translation="EPLVGSSRLLAVDFSLMVKHGLAPFYQWVNVDMYYLGIWRWLLS
                     LAGRIGRASLWLEERALATLLILTVATFALAALLIPELVSSTQAPRLAAHVAQAGGGT
                     GALMVPIGIALAGLLLVAHASARDSLRLPLLAVAGILALVGLGTSGEMIRLLLLESAS
                     LVALLLVWREGKNRSAAHAYLLAILLSAAATVGGTTLLGHGPATLAPALLLAGLTLKF
                     ALFPLYLWLPKMAESTPAPIAGLIVAVVDVAGFGEVLTLQATAPSIFVPVAPWLVVGV
                     LSALTGGVLMLAQRDVKRMLAFSTIEDMGVLLLGISLGGPLGTAGAVVGLIVHSLAKA
                     LLFSSLAIPELTAPVTLGSSKGLTARFPLSGAGFLLGALAVLGVPPTLGYAAHWRLYS
                     AAVTASPLLLAVLMLSTAMALLSYARIVGRCWWGPCEDETNGGEPALLKLAVVGLGVV
                     LLAAGLWPSLMSAGL"
BASE COUNT         1744 a         3060 c         3084 g         1860 t
ORIGIN      
        1 gccatgaagg accgccaggt agatggcttt gccgttaccg gtggcgttcc cacctccgcg
       61 atcaccgacg tgaccaccgc catcgacatc tcgctgctgc cgatcacagg cgacgatgcc
      121 aagaaggtga tggacaagta caagttcttc gttccggtca aggtgcctgc taccgcatac
      181 aaggggctag caggcgagac ttcgaccatt gccgtccagt cgatgctggt ggctcgggac
      241 gacctggatc ccgatctggt ctactggctg accaagaccc tgatcgagaa gcaggcagat
      301 ctggcccagg cccacgccaa gggcaaggaa ctctccaagg agtccgccgt caaggggttg
      361 accgttccgc tccatccggg cgccgagaag tactacaaag agatcggtgt catcaagtag
      421 gctgtccagc agtggtaggg gtgcactcgc ttgcacccct accaccctta tcctggatct
      481 aacccgttct tagaaggggt cccggtgcac ttgctccatt tcaagcagta ttcggccgct
      541 tcgaggggcc gactgttcct cctggtcccg ttggccctgc tggcgctcgc tgccctggcc
      601 gcctgggctc tctggccgac gcctacgctc gatatccggg taggtcaaga ggcgcggttg
      661 gtgagatcga tccccgtgtc gaccggggag atgatcacct acacatacct ccactcggtg
      721 cagaaaaagc cgatagacga agtgatggag atcgccccca acggccacct cgtggtacgc
      781 gagactacct acgagatgac cggagccggc ttgccttccg acgtcctgga cggcgatttc
      841 atcatcgatc ccgccacggg aaagtggaag atcatcaata tgtcgcggga tattcccgag
      901 tggcatgtga gggtggcctt cacggcgcag caaacgttgg aggtgggtgg ggagaagttc
      961 cgcctggact ccctggcaac gcccacaacc gtgctgatcg tcggtgtggc caaccatccc
     1021 cacatagttg ctctcctggg attggggtga attcatcggt tgtaatcatc gaggaggtag
     1081 tatggcgaaa gaagtacccc ccccaagcct tcgagacggt ctcggaggag gaggtcgagc
     1141 ggattaccgc caagctcgac gtcggatctc gactgaggaa gttcgtcggg ccgtttggca
     1201 tattcatcat aatcatggcc gcggccatgt cgatctttca tctctacact acgttcttcg
     1261 ggttcttcga cgcgccgatc cagcgggcag ccttcttcat cttcgttttt gtgatggggt
     1321 tcgctctcta ccctgcacga ttcaaaggca cgctggatac ccgccgcatc ccgccctggt
     1381 acgacctgat cctgatgggg ctctcggcca tggtcggggg ctacatcatc gtcaacttcg
     1441 agaatatcgt cgagaacatg ggcaatccca cccagcttga cctgatcatg ggaagcatcg
     1501 cctgcctgct ggtgctggag ttgtgccgtc gggcagtcgg cccggtgctc atgttcatct
     1561 gcctcttctt catcctgtac gccttctggg gcaactacat tccaggcttc cttagccaca
     1621 ggggttaccc ctttacccgg gtggtcagcc acatgtatct gaccaccgag ggcatctttg
     1681 gggtggcgac gggcgtagcc accacctacg tcttcatgtt catcctgttc ggtgctttcc
     1741 tcgtgcgtac gggcaccggc cagttcttca acgacatcgc ggtggcgctg ctgggcggtt
     1801 actccggcgg tccggctaag gtggccgtct tcgcctccgc gctggagggc accatcaacg
     1861 gttcatccgt ggccaacgtg gtgggcagcg gttccttcac catccctctg atgaagtcca
     1921 tcggatacaa gccctacttc gccggggcgg tcgaagccgc ggcctccacc ggagggcaga
     1981 tcatgccccc ggtcatgggc gccggtgcct tcatcatgtc ggaaatccta ggcaggccgt
     2041 acatcgaggt ctgtatcgcc gcggcactgc ctgccgtcct ttacttcacc ggagtgctgg
     2101 tgggcgttca cctggaggcg aagcgcaccg ggctggaggg gcttccaaga aacatgctgc
     2161 ccagccctat gaaggtgatg aaggagcgtg gcgtgatgct gctgcccatt gtggcggtag
     2221 tgtggatgct gatgaacggc tacacgcctc cgtacgcggc cgtgaccggc atcatcgcca
     2281 gcatcgtctt cggcgcgatg ctgaagacca cccgcgtcca tcccatcaag tactgggagg
     2341 cgctggtcgc cggcgcaagg gatgccctgg gtgtaagcat cgcctgtatc tccatcggtt
     2401 tcatcatggg ctcggccacc ctcaccgggg ctgggctgaa gatcgccaac agcatcgtga
     2461 tgctctccgg cggcaacctg ttcctgaccc tcttcttcac catgatcgca tcgctgatcc
     2521 tgggcatggg gcttccgacc acggccaact acctggtcac ttccaccatc gcggcaccgg
     2581 ccatcgtcca ggcgggagtg ccgttgctct cggctcatct gttcgtcttc tacttcggca
     2641 tcgtcgccga cctgacgccg ccggtggccc tggctgccat ggccggtgca ggattggccc
     2701 gcgctccacc cacgaagacg ggcgttcaag ccttcagatt ggcggcacca gcgtaccttg
     2761 tcccatacgt gttctgttat gctccacaga tgacgctgct ggggtacgac ttcataagcg
     2821 gggtggaggc atccgtcaca tctgtgctcg gggtgattgc cctgggtgtt ggcctgtttg
     2881 gctggatgat gacctgggtt ccgtggtggc agcgagtccc gatgctcggc gcggcgttgc
     2941 tgctgatcac cccgggttgg gagacggacc tggcagggtt tatcattctg gccgcggtct
     3001 tcatgcacca gcgctacctg gtcaagagcg gcaaggcgaa gccggtggag cgaaccagca
     3061 tccttcctgg gcagacgccc gggtcgcctg cctgatccaa gaaactggct gccgacgacc
     3121 tacgtcgggc aaacaaaagg gggcgtgcat cgcgcacgcc cccttttgtt tgcgttcctt
     3181 actcccagcg ggtcagcacg tccgggccgt tgttggtgat cgccacggtg tgctcgaagt
     3241 gggccgacca cttgccgtcc ttggtaaccg cggtccaatg gtcgtccagg acggtgatct
     3301 ccggcgaccc catgttcacc tgcggctcta tggccagcac cattccaggg cgcagcagcg
     3361 ggccctgatg cggcggaccg aagtggggaa cctggggagc ctcgtgcatg ttgcgtccca
     3421 ccccgtgccc cacgaaggcg cggactacgg ataatccgtt cttctccacg tggcgctgga
     3481 tggcgtgcga aatatcggag atccggtttc ccactatcgc cttatctata cccttgaaca
     3541 gtgcttcctc ggtgaccttc agaagccgtg caacctcggg ccgtacctgg cctaccggat
     3601 aggtcatcgt ggagtcggca aagtagccct tgtgggaggc cgccacgtcg aacttgacga
     3661 tgtcaccctc gttcaattgc cggctgcccc tgatgccgtg gacaacctgc tcgtttatag
     3721 acacgcaaat gttgccggga tagccgtaat agttcaggga gccggagacg gcgtcgtgct
     3781 cacggaagat ctttgccgcc aggtcgtcca gttcctgagt ggtgatcccc ggctgcgcgg
     3841 ccttcttcag ttctttcagg gtgaggacga cgatctttgc cgcctcacga atcttctcga
     3901 tctctttggg cgatcgcagt gtgattgctt cgctttctga catagtacta tcgctcacca
     3961 attcctacag tcttaacatc gaagacaggc aacattgaac tataccataa ccggcggacc
     4021 gtctgctccc gggacgcgac gacgacccgt atcgcgcgcc gataggtctg tccctaccac
     4081 ggaagtcgtt gcggtggacc tcgtcagcgt ctacctgtag aatttccacg aagcatgccg
     4141 tccgcagcct tgccatcgaa ggccgtccgc ttgctcacta ctgatagctg aaggctgagt
     4201 gccaccttga agcattacct tatcggactc accggaaaca tcggctgcgg caagagcgcg
     4261 gtagctgcga tgcttgcaga cctcggtgcc gaggtggtgg atgcggatct gctggtccac
     4321 cagttgatgt cgccggggag cgaaagctgg caggcaatcg tcgaccgctt tggcaggggc
     4381 atccttcggc ccgacgacgg catagatcgg cgtgcgctgg gcgagatcgt gttcagggat
     4441 ccggccgcac tggcctatct ggaggcgata ctgcaccccg cgacgcgacg gctggtggag
     4501 gagcggattg ccgcttccaa gagcgccgtc gtggtggtgg aggcgatcaa gctgatagag
     4561 tcgggctggt accgaagctt ggacgccctc tgggtggttg cgtgctcgcg ggagcagcag
     4621 atagagagga tcatgcgaac cagaggcttc aagcgcgccg aggcggaatt gcgggtggac
     4681 gcccagcccc cggccgagga gaagttgcgc tacgccgacg tgatcatcga caacagcgga
     4741 tcgctggaga cgacccgccg gcaggtagcg gatgcctggc agcggatccc gatggtcccc
     4801 gatgccggct gatcctggta gggcgggtcg cccctgaccc gcccgtctct cttctgatgg
     4861 tggctcgcgg cctgggctgt tgttgctgaa cggcaagaag gggggccgga ctatcggccc
     4921 ccccttcttg cttctgcttc ttcgcctacc tgtcggtgca ggagtagcag ggatctattg
     4981 atgcctggat gagcggcacg tctgccagcg tcgctcccag caccatgggc cgtgcagcgg
     5041 tcaggttgag gtaggtgggc gtgcgagcct taacccgggt gggaatgtcc gatccgtcgg
     5101 atgcgacgta gtagaagacc tctccacggg gcgcctcggc tctcgcaatc gcctcacccg
     5161 gcggcacctg gacgaactcc gggccgcgca aggggcccgg aggcatcagc agtaccgcct
     5221 gctccaccag actgcagctt tcgaggatct ccaacgcccg caccactacc ctggcgacca
     5281 cgtcgccgct ctcctgcacc ggcacctgga actccagccg gtcgtaggcg gcgtaggggg
     5341 tagccttccg aatgtcctgc ttcaccccgg aggcacgagc ggtagggccg accgtgccga
     5401 gagccactgc ctgttcgtgg gtcagcacgc cgacaccggc agcccgcgcc ttgatggtgc
     5461 gatcgccggt gaatatcggg atcatggtgc gctccacctc ccggcggatc tcccggaccc
     5521 cattgaggac cgcatccgga tcggtgatgt ctcggttcac cccgccgatg cagttcatgg
     5581 cgtagttgac ccggttgccc gagatcgcct ccaggaggtc catcaccttc tctcgcagct
     5641 gccagcagct catgaacagg gtctgaaatc ccattacctc ggcggcgatc cctgcccata
     5701 ggatgtggga gtgaagcctc tccagctctg ccaccaccac ccggatgtag cgggcgcgct
     5761 ccgtcggttc cacaccggcg agccgctcta cggccatgca gtaggtcatc gtgtggacgt
     5821 tggagcagat gccgcatatc cgctccacca acgcgatgtt ctggatgtag ttcttctgct
     5881 gagccaaccg ctctacgccc ctgaagacga agccgacgtg gagctgggca tcctttatga
     5941 cctcgccctc gcaggagatc tcaatcttgc acggctcctc gagagcgggg tgatagggtc
     6001 caatcggtat tgtgtaggtc atgattgctc tccatcctgc cccttccaga agaagaagcc
     6061 cggtgggtcg ttggggcgta gggtgagggg cggtgtgctc tggcggcctg cgaaggagac
     6121 cgcgaagtag tcgtggatct cgcgctctat ccaatcggcg gcagggcaaa tgtccacgat
     6181 ggtgtctatc gtgttctggt gggtcccggc gatcagtgtg gttacctgcc cctccaggtc
     6241 ccagtgatag gccaatcgct gctcgccgcc ggcaacgggc gtcgctgtta ccgtcatcag
     6301 ccttgcgccg ttccggacca tgcagcgggc catgtctcgc accttgagga gcgggtcctc
     6361 cagccagtat ccgtcgggac gcttttccca cggttgtgcc gtgcccagct gggcgcggat
     6421 catctcgacg tccatctagt tgtctccttc agcgtgcgtt gtgtatgccg tttccggcag
     6481 cgatcgcaca gccggcttcg gctgcgtgct tcgtcggtca gttccgacag cgccttcgcc
     6541 agcagttcgt cgctgatcgg cagagccggt ctgccgcatt gtgggcagag cggatagtgc
     6601 acctggtgcg tctctgccag tgcgccgggc gtggtgtaca ccggcgccgg ctcctctttc
     6661 atggagaggg ccttgcccat gcagaggtcg gcacagcggc cgcagaaggt gcagcgaccg
     6721 gggtcgtagc tccagtcaca gctgtcggtc cggtcagtga cggtgattgc tgcagacggg
     6781 caaacgtagg cgcagacgcc gcaggcgagg catcgagcgg tatcgatttg gaccgggccg
     6841 cggtaatggc tcggcggtgc gacccttgcg gggaagcgca gggtgacggg gccctgccgt
     6901 agattggcga caatcacgtt cagtatgttc atcgtctcag cctctcagtc cgggcaacgc
     6961 tgccatgacg agtgccagca cggcgatccc catcccccat ttccagtaga cgcgggacgc
     7021 ctgagtcact ttgagccgtg cggttgctgc tgccagggta gtcaggataa gcaccaaaag
     7081 catagagaga acgacgaata gtgcgacgcc gacgatccag ttcgtcgggt ggaccggcag
     7141 cccgagggtg atcgccaggc cggtaagtgc gacccattcc aggccgtggg cgagctccca
     7201 gatggccagg gagcgaccgt cgtactccgt cgtcgggccg gcgtagatct cctgctctgc
     7261 attaggtatt gagaagggat tgaggcgcag cttcaccggt atgcagagca gcagcgccag
     7321 gatcgccggc aagcgaacca gggggggggg gtgagcaggg ccacatcgga gagcctgagg
     7381 ctccctgccg cggcggccag cgccatcagc gcggcgagga acggcaggtt gtagctgata
     7441 ctgagcacgg cctctcgcac cgcacccacc tgtccgaaga tggatctgga tgcgaagcct
     7501 gccagcacgg agcagatggc gggcaattcc agcagtgcca ccagcaacac catgtcgccg
     7561 gtaaaaccgg tgctgcccgg gattaccggc aatagcgcca gggaaccgag ggcggctccc
     7621 actgccagca ccggcaaccc ggtcatcagc atcccttcaa gggggtcgcg tgttatcggt
     7681 cgcttggcca tcagcttcac gaagtcgaag aagggctggt agaagggggg gccgatgcga
     7741 ccctgcattc gggccgtcaa cttccgctcc atccacagca tcaaccaggc tacaggtacg
     7801 gcgtacagca acccggggaa gatcagtaac gccagtagac cctgtatgag gttcattgtt
     7861 gggcctcctg cttggctacc aggatcttca ctgcctcggc gatcccggcg atgatggcat
     7921 cggggcgagg cgggcagccg ggcacgaaca cgtccaccgg tatcacctcg tttaccggcc
     7981 cctctacggc ggggctgccc gcatagacgt tgcaggatgc cgggcaggtg ccgagggcaa
     8041 ccaccacctt aggatcgggc atctgcgcgt agaccgtctc caaggcatgt ctggtgttgc
     8101 gagtgaccgg cccggagacg cacagcacgt cggcatgcct cgggctggcc tcgagccgga
     8161 tgcccagctg ctccacgtcg tagcgtggcg tcaggcaggc caccagctcg atgtcgcagc
     8221 cgttgcatcc gccggtattc aagtgaaaca gccagggcga cttgcgccgc gccgagccga
     8281 ccagttcatc aattagtccc ataaggcacc ttccattttg tctcacttgg tgtcgctgtc
     8341 gacggctcct ctagagtccc gccgacatca gggacggcca gaggccggcc gccagcaaca
     8401 cgacccccag ccctaccaca gccagcttta gtagcgcggg ttccccgccg ttggtttcat
     8461 cttcgcaggg cccccaccag cagcggccca cgattcgtgc gtaggagagc agcgccatag
     8521 cggttgagag catcagcacc gccagcagca gcggactggc cgtgaccgct gcgctgtaca
     8581 gccgccagtg agcggcgtat ccgagggtcg gcggcacccc aagcacagcc agcgcaccta
     8641 gcaggaagcc ggctccggag agtgggaagc gggccgtgag tcccttggag cttcccaggg
     8701 tgacgggagc cgtcaactcg gggatggcga gactggagaa gagcagcgcc ttcgccaggg
     8761 agtggacgat gagcccgact acagctccgg ctgttcccaa cgggcctccg aggctgatcc
     8821 ccagcaagag cactcccatg tcttcgatag tggagaaggc cagcatccgc ttgacgtccc
     8881 tctgggccag catcagaacg ccgcccgtga gggccgacag gacccctact accaaccatg
     8941 gggcgaccgg aacgaatatc gacggtgcgg tggcctgcag tgtgagcact tctccgaagc
     9001 ccgccacgtc taccacagcg acgatcaagc ccgcgatggg tgcaggggtg gactctgcca
     9061 tcttgggtag ccagagatag agcgggaaga gtgcgaactt cagggtgaga ccggccagca
     9121 gcagcgccgg ggccaatgta gccggtccat gcccgagcag cgtggttcca ccaaccgtgg
     9181 ccgccgcgga gagcaggatg gccagcaggt aggcgtgtgc ggcggaacgg ttcttaccct
     9241 ctcgccagac caggagtagc gccaccaggg aggccgactc cagcagcagc agccggatca
     9301 tctcgccgct cgttcccagc ccgaccaggg ccaggatgcc tgccacggcg aggaggggca
     9361 gcctcaggga gtctcgagcc gaggcgtgag ccaccagcag gaggccggcc agtgcgatgc
     9421 ctatcggcac catgagggcg cctgtgccgc cgccggcctg ggctacgtga gctgcgagcc
     9481 tgggcgcctg ggtagaggag accaactccg gaatcagcag cgctgccagg gcaaaggtgg
     9541 caacggtcag gatcaggagg gttgccagcg cccgctcttc cagccagaga ctggcacgac
     9601 ctatcctccc tgcgaggctg agcagccacc gccagatccc caggtagtac atgtccacgt
     9661 tgacccactg atagaagggg gccaggccgt gtttgaccat cagggagaag tccaccgcca
     9721 gtagccgact ggacccgacc agcggctc
//
LOCUS       DATALS010000177         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_241955, whole genome shotgun sequence.
ACCESSION   DATALS010000177 DATALS010000000
VERSION     DATALS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241955"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VHS06_03935"
     CDS             <1..203
                     /locus_tag="VHS06_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987302.1"
                     /translation="KAITRVVFANQQLDGFWPKAGGYSTVGTIAEGYDHPLDLPGGQQ
                     ADRGGRGDLSGRKRGAGGSAAG"
     gene            complement(214..>1621)
                     /gene="rfaE2"
                     /locus_tag="VHS06_03940"
     CDS             complement(214..>1621)
                     /gene="rfaE2"
                     /locus_tag="VHS06_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX2987303.1"
                     /translation="ERVAEILNQNFVSIKVDREERPDIDQIYMAALQAMTGGGGWPMS
                     LFLTPEGQPFFAGTYFPPRFSHGTHSFTDVLTSVIDAWQYRREELQRGGEQIVAALLA
                     HEEELEGATAEIGQEILARAYEAILRDFDAARGGWGEGPKFPQPMVLEFLLRHHLATG
                     LSEPLEMATQTLDAMARGGIYDQLGGGFHRYSVDGNWLVPHFEKMLYDNAQLARVYLH
                     GWQLTNALPVATGEAPEVDLVARCRKSDGVRKMQGYRAVRYHTDCRCRSGYPTGDVGA
                     DPAYPRRGVGTGLRARPRNAVRRSGFRGGGPVGKVLDRESLIQKVAALKAAGKKIVFT
                     NGCFDLIHTGHVRYLREAASFGDILVVGLNSDASIRLFKDPGRPLVPEADRAEVLAAL
                     EMVDYVSIFEERTSEALVAVVRPDIYVKGGDYRSEDLPEARIVDSYGGRVQLVGYVEG
                     RSTSQIVRDILESYCPKQ"
BASE COUNT          289 a          530 c          516 g          286 t
ORIGIN      
        1 ccaaggccat aacccgagtc gtattcgcca accagcagtt ggacggcttc tggccaaagg
       61 cggggggtta cagtacggtc gggaccatcg ccgagggtta cgaccatccg ctggacctgc
      121 cgggggggca gcaggcggat agaggggggc gaggtgatct atccggacgg aagagagggg
      181 cagggggaag cgccgcgggg taggcgatgc tgcctactgt ttgggacaat agctctccag
      241 gatatcccgg acaatctggc tggtggagcg accctccacg tagcccacca gttggacacg
      301 gccaccatag ctgtcgacta tccttgcctc cggcagatcc tcggagcggt agtcgccgcc
      361 cttcacatag atgtccggcc ggacgaccgc taccagcgct tccgaggtcc gctcctcgaa
      421 gatggagacg tagtccacca tctcgagagc ggcaagcacc tccgccctgt cggcctccgg
      481 tactagcggc ctaccggggt ccttgaacag ccggatagag gcgtcgctgt tgagcccaac
      541 cacgaggatg tcgccgaagg aggcagcctc ccgcaggtag cggacgtggc ctgtgtggat
      601 caggtcgaag cagccgttgg tgaagacgat cttcttaccg gcagccttca acgcggccac
      661 cttctggatc aatgactccc tatccagcac cttacccacg ggtccacctc ctcgaaagcc
      721 gctccggcgg acggcattgc gcgggcgggc acggaggccc gtccctacgc cacgtctcgg
      781 gtaagccgga tcggccccta cgtcacctgt cgggtagccg gaccggcatc tacagtctgt
      841 atgataccga accgcccgat agccttgcat ctttcgcacc ccatcgctct tgcggcatct
      901 cgcgaccagg tcgacctcag gggcctcgcc tgtcgccact ggaagggcat tggtcagttg
      961 ccagccgtgg agatagaccc tggccagttg agcgttgtcg tacagcatct tctcgaagtg
     1021 gggcaccagc cagttgccat ccacggagta gcggtggaaa cctcccccca gttggtcgta
     1081 gatcccgccg cgagccatgg cgtccagagt ctgcgtggcc atctccagcg gctcggaaag
     1141 gccggtagcc aggtggtggc ggagcaggaa ctccagcacc atcggctggg ggaacttcgg
     1201 cccctccccc catccccctc gggcggcatc gaagtcgcgg aggatggcct cgtaagccct
     1261 ggcgaggatt tcctggccga tctccgcagt agcaccctcc agttcttcct catgggcgag
     1321 tagtgccgcc actatctgct cgccacctcg ctgcaactcc tccctgcggt attgccacgc
     1381 gtcgatgacg gaggtgagca cgtcggtgaa ggagtgggtc ccgtggctga agcggggcgg
     1441 aaagtaggtg cccgcgaaga acggctgccc ctcgggcgtc aggaagaggg acatcggcca
     1501 gcctccgccc cccgtcatcg cctggagggc cgccatgtag atctgatcga tgtccgggcg
     1561 ctcctcgcga tccaccttga tgctgacgaa gttctggttg aggatctcgg ccacccgctc
     1621 g
//
LOCUS       DATALS010000178         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_151548, whole genome shotgun sequence.
ACCESSION   DATALS010000178 DATALS010000000
VERSION     DATALS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151548"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VHS06_03945"
     CDS             complement(<1..219)
                     /locus_tag="VHS06_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987304.1"
                     /translation="MRADGMRGVLSEKARTWLLLQGFALALLTAACIPSSPTYPFDIF
                     PEMHYSPAVRQEEPPRLSVPPGAVPVTGA"
     gene            complement(209..730)
                     /locus_tag="VHS06_03950"
     CDS             complement(209..730)
                     /locus_tag="VHS06_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HEX2987305.1"
                     /translation="MAVTSILALYSDAVAAADGVIALHEAGLSDDNFELLTDTPYPEG
                     AFGEQPVHHRLYVFPLVGAFCGLVVAVLLTTGTQLAYPLITGGKPILAIPPMLIIWFE
                     GIMLGAIGMTFVGFFFEARLPRIHLGLYDPRIAEGYIGLLVSTDESIIGDAEKALRSS
                     GAVDVIRQGEGEG"
     gene            complement(798..2123)
                     /gene="nrfD"
                     /locus_tag="VHS06_03955"
     CDS             complement(798..2123)
                     /gene="nrfD"
                     /locus_tag="VHS06_03955"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX2987306.1"
                     /translation="MQERYDLSAQRINRDLLSFVRSTPWWFLLLFLLLGGGVAAMLAA
                     TGHMVYLGLVVTGLDRPVYWGFLITNFVYWVGMSQAGVMMSSILRLTQAEWRRPITRM
                     AEVLTVVLLLTASYFPLIHTGRPWRTLYWILPYDFPRNIWPNIRSPLIWDPAAIFTYL
                     FASSMIVLLSLIPDMAAARTRATGWQRHFFALLSFGFRGTPRQWRVHSLATVLMAAMI
                     IPVFVTVHSIVAWDFGVQLVPGYHSTVLAPYFVIAAMNSGVAAIITFMVFFRFWLRLD
                     RYITADHFDAIGRLQIAVAVTWLFFYFLDFFGGMVSQEAREVAVWQLRLTVWPYPFML
                     AVMFLTSFFIPVPLLLIRRVRRNAAAILWIALLINVGMWIERLTVVVPPPCVQAALHL
                     RLGLVRADLGRDHHHPRIGCFRRVRNAGVGQDRTDRPPLRRKGGAGSEG"
     gene            complement(2134..>2810)
                     /locus_tag="VHS06_03960"
     CDS             complement(2134..>2810)
                     /locus_tag="VHS06_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987307.1"
                     /translation="LVPGWLSSIFPAFQITTAFEAAVAVDVLALYIWRRWGGLGRYIR
                     PDVFSGTGGILFCLALLSLYMWWSAFITQWYGRTPREQNLLFFQEFGVYFVPWVIFFL
                     LVIVIPIAVLLWRPIRHSILGPTFVSLGIIVGVFFDRIRAYVGAFTITDVTAQELQQL
                     PPARLPDALDILMVLGSLAGAVLLYMLGAKLVPPLSIWEIKEGLLLRLHRPFLRGEYS
                     VLGKPR"
BASE COUNT          618 a          811 c          941 g          440 t
ORIGIN      
        1 cgcccccgtt acgggcaccg cgccgggcgg gacggagagc ctcggcggct cctcctgccg
       61 aacggccggg gagtagtgca tctcggggaa gatgtcgaag ggataagttg ggctgctggg
      121 tatgcaggcc gcggtgagca gcgccagcgc aaatccctgc agcagcaacc aggtgcgagc
      181 cttctcagag agtaccccac gcatgccgtc agccctcacc ctcgccctgc ctgatcacat
      241 ccaccgcccc gctggagcga agcgccttct cggcgtcgcc gattatgctc tcgtcagtgg
      301 aaaccagcag gccgatgtag ccctcggcga tgcgtggatc gtacaggccc aggtggatgc
      361 gcggcagtcg tgcctcgaag aagaaaccca cgaaggtcat gccgatggcg cccagcatga
      421 tcccctcgaa ccagatgatg agcatgggcg ggatggcgag gatcggtttc ccgcccgtga
      481 tcaacgggta ggcgagttgc gtgccggtgg tcagcagcac cgcgacgacc agtccgcaga
      541 aggctcccac cagcgggaag acatagaggc ggtggtgcac aggctgctct ccaaaagccc
      601 cctccgggta gggcgtatct gtaagtagct cgaagttgtc gtcggagaga cctgcctcgt
      661 gcagggctat caccccgtcg gcggcggcca ccgcatcgga atagagcgcc aggattgagg
      721 ttactgccat ccgctcgcct ctcagtctct aatgactctt gatgatggcc gggacctctc
      781 tcttgcctac gtcgatctca gccttcagaa cctgcccctc ctttgcgtcg aagaggggga
      841 cgatcggtac gatcttggcc aacaccagca ttccgaaccc ggcgaaagca accgatccga
      901 gggtgatgat gatctcgacc caggtcggca cgtaccagcc ccagacgaag gtgaagggct
      961 gcttgtacgc aagggggggg gacgactact gtgagccgct cgatccacat ccccacgttt
     1021 atcaggaggg cgatccacaa tatcgctgcg gcgttgcggc ggacccgacg gatcagcagg
     1081 agcgggaccg gaatgaagaa ggacgtcagg aacatcactg ccagcatgaa tgggtatggc
     1141 cagacggtga gccgcagttg ccagacggcc acctctcgtg cctcctgaga gaccatcccc
     1201 ccgaagaaat cgaggaagta gaagaagagc caggtgaccg cgacggctat ctgtagccgg
     1261 ccgatggcat cgaagtggtc ggcggtgatg tagcgatcga gccgcagcca gaagcggaag
     1321 aacaccatga aggtgatgat ggcggcgacg ccggagttca tggcggcgat gacgaagtag
     1381 ggcgccagca ccgtggagtg gtagccggga accagctgta cgccgaagtc ccacgcgacg
     1441 atgctgtgaa ccgtcacgaa cacagggatg atcattgcgg ccatgaggac agtggcgagg
     1501 gagtggaccc tccattggcg gggcgtcccg cggaagccga aggagaggag ggcgaagaag
     1561 tgccgttgcc agccggtggc tcgtgttcgg gcagcggcca tgtccgggat cagcgacagc
     1621 aagacgatca tgctgctggc gaacagatag gtgaagatgg ctgctgggtc ccagatcagg
     1681 ggtgatctaa tgtttggcca tatgttacgt gggaagtcgt agggcaggat ccagtagagg
     1741 gtgcgccagg gccgaccggt gtggatgagc gggaagtagg aggcggtgag cagaaggacg
     1801 acggtgagga cctccgccat gcgggttatg gggcgccgcc attccgcctg ggtcaagcgc
     1861 aggatggagg acatcatcac ccctgcctga ctcattccca cccaatacac gaagttggtg
     1921 atcagaaagc cccagtagac gggcctgtcc agccccgtga ccaccagtcc caaatagacc
     1981 atgtgtccgg tggcggccag cattgccgcc acaccacccc ccagcaggag gaaaagcaga
     2041 aggaaccacc acggggtcga gcgcacgaaa cttaggaggt cgcggtttat tcgctgggcg
     2101 ctcaggtcgt agcgttcttg catgtcagat cacctatctc ggtttgccga ggacggaata
     2161 ttcccctcgg aggaacggcc ggtgcagcct cagcaggagg ccctccttga tctcccagat
     2221 ggaaaggggc ggaaccagct tggcccccag catgtagagc aaaacggcgc cggcgaggct
     2281 acccagtacc atcaggatgt cgagggcatc gggcagacgg gccggcggga gctgctgcaa
     2341 ctcctgtgcg gtcacgtcag tgatggtgaa ggcgcccaca tacgctcgga tccggtcgaa
     2401 gaagacgccc acgatgattc ccagtgaaac aaacgttggg cccagaatgc tgtgccggat
     2461 cggtcgccag agcagcaccg cgatcggaat gacgatgacg agaaggaaga agatgaccca
     2521 cggcacgaag tagacgccga actcctggaa gaagaggagg ttctgttctc gcggggtgcg
     2581 tccgtaccac tgggtgatga aggccgacca ccacatgtac agggagagga gggcgaggca
     2641 gaagaggatg ccgcctgttc ccgagaacac gtcgggacgg atgtagcgac ccagtcctcc
     2701 ccatcttctc cagatgtaga gggccagcac gtcgacggcg acggcggcct cgaaggcggt
     2761 ggtgatctga aaggccggga atatcgaact cagccagccc ggaaccaggg
//
LOCUS       DATALS010000179         5050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_17796,
            whole genome shotgun sequence.
ACCESSION   DATALS010000179 DATALS010000000
VERSION     DATALS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17796"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VHS06_03965"
     CDS             <1..202
                     /locus_tag="VHS06_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX2987308.1"
                     /translation="AVIATLIVYCLKGVDFAQFPSGIAELIAIAVVVLLHLWRRSFLL
                     SIGAGTVCYMVLVQRVFVVPLP"
     gene            753..1994
                     /locus_tag="VHS06_03970"
     CDS             753..1994
                     /locus_tag="VHS06_03970"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX2987309.1"
                     /translation="MTYRKLYRARTLRIAVLILSVVLTGLLAGFSPRGSESSLTEIKT
                     FEGGTAYRTSDDAYWVLSLNGTWREMGRQYGGLVGDDLRQFYADISADVATRGMDYDK
                     QLENAKAIADGLSSNLNELLKGMAEASDLSEDEVLVLNAGMANLAIKAMGIEIPSSCS
                     GLAAWDRYTPNGTLVFGRNWDMDRESMGKYMKYLSVVVFNPDSGNSFANVHPLGSVFL
                     ETGMNDKGLFVELNNGGSSDPHYVEGREDSASVLATLLNEYSSLDEASKYLSEVPADY
                     SYIIQLADTKEAVSVERPTFGTRVRRSDQNGVLAAYNSFIPPYPKEWEGKVADPPKEE
                     DDPRYSNLITLANSKEFFGNLDADGMKRLMGIWIRDGGAMHEGSVYQVVAVPQEFRLW
                     IRGLDYSDWQQVDLKDLFTNK"
     gene            2251..2646
                     /locus_tag="VHS06_03975"
     CDS             2251..2646
                     /locus_tag="VHS06_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2987310.1"
                     /translation="MDMESTVLLALLSLAVVGTILMIFVPAKGRRGMTASIKADGVQE
                     IEIVVKGRYRPDTVVVRKGVPARLLFNRQEDTPCSERVIFSEFRQERWLAPYATTAIE
                     FTPTQKGDFLFTCARGMYQGRMLVEDSRH"
     gene            2656..3513
                     /gene="lgt"
                     /locus_tag="VHS06_03980"
     CDS             2656..3513
                     /gene="lgt"
                     /locus_tag="VHS06_03980"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX2987311.1"
                     /translation="MGPIFVNVDPTIVRLGHFSLRWYGLFIGLAIVAAFLVALREAHR
                     KGVAEDDVYGLGMWAVLGGLVGARLLHVIDRWPDYAQDPVSILYIQNGGLAIYGAILG
                     GVAAGAVYSRRHGLSICRMADLAAPAIVLGQAIGRIGCLINGDALGSPTNLPWGVAYL
                     NPEAMAPSLGVAYHPTPAYEMIGDILIFALLWRLRHRVKAEGVLFLVYLALYSTLKFS
                     VTLVRQEVVWLGGLQEAQLVSMAGGLVAVALLVYLSRKHAITAQPEMQSSERGLERSG
                     SARNNRRSR"
     gene            3750..4709
                     /locus_tag="VHS06_03985"
     CDS             3750..4709
                     /locus_tag="VHS06_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HEX2987312.1"
                     /translation="MARQLFVRQGAKPLITPADLPFEANAVLNPGVAELDEGVVLLLR
                     IEDATGVSHIHVARSTNGVDGWRVDERPLLQANLPEYPFEEWGCEDPRVTRVGPEKWI
                     IAYTAYSRYGPAVALAATENFEAVTRLGVILSPTNKDATVFPEPFDGMWILLHRPVTG
                     GQEHIWYACSPELTHWSLPGVLLPQRSGPWWDGLRVGVGAPALRTDQGWLLIYHGVKD
                     MAGRPIYRLGLALLDLENPRKVLARASRWVFAPDTDYEQRGLVPGVVYTCGALLRGDE
                     VWMYYGAADTVIGLAIAKVSDLLAFVHQHDFLHQVGREKGTAS"
     gene            4782..>5050
                     /locus_tag="VHS06_03990"
     CDS             4782..>5050
                     /locus_tag="VHS06_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987313.1"
                     /translation="MAAHRKSGLYRPSLVGVLATGLLVLVLAKWPTWRRDPNRLFRAM
                     YNLVAPIYDLWAGEKSALAPLRQQLAEMMELEPGDQVLEVGAGTGA"
BASE COUNT         1037 a         1402 c         1569 g         1042 t
ORIGIN      
        1 agccgtcatc gccacactca tcgtctactg cttgaagggc gtagacttcg cgcagttccc
       61 tagtggtatc gcggaactga tcgccatcgc cgttgtggtg ctactgcact tatggagacg
      121 gagcttcctt ctgagtattg gcgcaggcac ggtgtgctac atggtgctcg tacagcgtgt
      181 attcgttgtg cccctaccgt agcctcacac taagcggctt tccctggatg caatcaatcc
      241 ttccatgcgg ggaaagtcga tacccacaag ggaaagtcgc ttagagcgcc cggttggcct
      301 ccgacagaca ggcgaatgat cattcgaccg tcgaggagcc aatgcgcagc cccgcctatg
      361 atcggcctac atagccggtg caggaccggc gtctacgctc tctccccact caccccaact
      421 ctgcccaacg ctcgcctgca agtccgcggc cgtgtacatc ctccacggtg tctgctgctc
      481 accccccaat cgcccctact gcgaccatgt aagggttcag cgtgccacgc ggtctgccga
      541 tataaaggat ggaggtacaa gagccgtgag acggctcttg acgcgcatgc tgacacggca
      601 tgccctccca aacctggtag ttgccgatgg tagagagact cgaacaaagg gtagtgatga
      661 tcaagtctat ggtgggcagg ctgctacgga ggccggtcta cctgatactg gtggcgggac
      721 tcgtcgccgc attcgaagga gaatctacaa ttatgaccta tcgtaaactc tatcgtgccc
      781 gcactcttcg gattgccgtc ctgatcttat ccgttgtttt gacaggccta cttgccggtt
      841 tcagccctcg gggaagcgaa agttcgctga cggaaatcaa gacatttgaa ggtggcacag
      901 catatcgaac cagtgacgat gcttactggg tgctctccct gaacggcacc tggcgagaga
      961 tggggcgtca gtacggaggg cttgttggag atgacctgcg ccagttctat gcggatatct
     1021 ccgcagacgt ggcaacgcgc ggaatggatt atgacaagca gcttgaaaac gcgaaggcca
     1081 tagccgatgg tctcagctcg aacctcaatg aattgctcaa aggtatggcc gaggcatcgg
     1141 acctcagtga agatgaggtg ctagtcctga atgcagggat ggctaacctg gcgatcaaag
     1201 ccatgggtat tgagatcccc agttcgtgca gcggtttggc ggcctgggat aggtacaccc
     1261 cgaacggcac gctcgtgttt ggcaggaatt gggacatgga cagagagtct atggggaaat
     1321 acatgaagta tctctccgtg gttgtgttca atcccgattc tgggaacagc tttgcgaacg
     1381 tacaccctct cggtagtgta tttctcgaaa caggcatgaa tgacaaaggg ctattcgtag
     1441 agctgaacaa tggaggatcc tccgaccctc attacgtaga aggtcgcgaa gatagtgctt
     1501 ctgtgctggc aacgctgctc aacgaataca gcagcctcga tgaagcgtcg aagtacttgt
     1561 cagaagtccc tgccgattat tcgtacatta tccagcttgc cgatacgaaa gaggccgtat
     1621 cagttgagag gccgactttc gggacccgtg tgagaaggag cgaccagaac ggcgtactgg
     1681 ccgcatacaa cagtttcatc ccaccgtacc cgaaggaatg ggagggcaaa gtagcggatc
     1741 cgcccaagga agaagatgac ccgcgataca gcaatctcat tacactagcc aactccaagg
     1801 agttcttcgg caatctcgat gccgacggca tgaagcggct catgggtatt tggataaggg
     1861 atggcggtgc tatgcacgag ggctctgtat atcaggttgt cgccgtaccc caagagttca
     1921 ggctgtggat tcgtggtctg gattattccg attggcagca ggtcgatcta aaggatctgt
     1981 tcacgaacaa gtagagacta agtctgcgga aaagggctaa agcgagaggc aaccggggcg
     2041 attatgatgc cgccccagtt gcctctcgct tcttgatggg gggagttgcc gggtcgatcg
     2101 gccatccatg agtatttcgc ctgaaaggca agagccacaa ctctaagccg tctcccaatt
     2161 ggcattcagt ccaaattaag gaatggcccg tacactgacc ggtgcagggt gacttacgcc
     2221 tcttgggtct caacactgga gatgaatgcc atggatatgg aatcgaccgt tctgctggca
     2281 cttctcagct tggccgtcgt cggtacgatc ctgatgatct tcgtgccggc caaaggaaga
     2341 cgagggatga cggcttcgat caaggctgat ggcgttcagg agatagagat agtcgtgaag
     2401 ggtcggtacc ggccggacac cgtggtagtg agaaagggag tgcctgctcg cctactcttc
     2461 aaccgccagg aggacactcc atgctcggag cgcgtgatct tctctgagtt tcgacaggag
     2521 cgatggctgg caccctatgc gaccacggcc atcgaattca cccccacaca gaagggcgac
     2581 tttctgttca cgtgcgctcg cggcatgtac cagggccgaa tgcttgtaga ggattctcga
     2641 cactaggagg caatcatggg tcccatattc gttaacgtcg acccgacaat cgtgcggctg
     2701 ggccatttct cccttcgatg gtatgggctg ttcatcggcc ttgccatcgt ggccgctttt
     2761 ctcgtcgccc tcagggaggc ccacagaaag ggcgtggccg aggacgacgt ctacggactc
     2821 ggcatgtggg cggttctggg aggcctcgtt ggggccaggc tgctgcacgt gatcgatcgc
     2881 tggccggact acgcgcagga tccggtgtcg atcctgtaca ttcaaaatgg aggtctggcg
     2941 atatacggtg ccatccttgg gggcgtggcc gcgggcgcag tctattcccg acgacacggt
     3001 ctgtccatct gcaggatggc ggacctggcg gcccctgcca tcgtgctggg tcaggcgata
     3061 ggccggattg gatgcctgat aaacggcgac gcgttgggct cacccaccaa cctcccctgg
     3121 ggggtggcct acctcaaccc cgaagccatg gctccgagcc tgggcgttgc ctaccacccc
     3181 actccggcct acgagatgat aggcgacatt ctcatctttg cactgctgtg gcgcctgcgg
     3241 catcgcgtga aggcagaagg agtcctcttc ctggtctacc tcgccctcta ctcgacgctc
     3301 aagttcagtg tgacactggt gcgccaggaa gtagtgtggc tgggcggact gcaggaggca
     3361 caactggtat cgatggcagg tgggctggtt gctgtagcgc tgctggtcta cctgtccagg
     3421 aagcatgcca ttaccgctca gccggagatg caaagcagcg agcgcggcct ggaacggagc
     3481 ggcagtgccc ggaacaacag gcgctcgcgg taacccataa gagggcgata gtggcaataa
     3541 ccaatgcgga ggcactcaca taatgaaccg gcttggaccg cgtaccggct actggattct
     3601 catggtgttg tccgttgtga tgatgttgtg ggttatgcac gggcagcaac cctgatgatg
     3661 ccgatcgaca tggctccgca cagcaccaac tcgtgccagg cattggtctt gcggccacga
     3721 tgggtgtaaa gaggtgttga ggatgaggga tggctcgtca actgttcgtg cgccaggggg
     3781 ctaagccgct gatcacgccg gccgatctcc ctttcgaagc caacgcagtg ctcaacccgg
     3841 gggttgccga actggatgag ggagtggtgc tgctgctacg gatcgaggat gcaacgggcg
     3901 tttcccacat ccatgtggcc agaagcacca acggggtgga tggctggcgc gtggacgagc
     3961 ggccgctgct gcaggcgaac ctgccggaat accccttcga ggagtgggga tgcgaggatc
     4021 ctcgggtgac tcgcgttgga cccgagaaat ggatcatcgc ctacactgcc tattcccgct
     4081 atggacccgc ggtggccctt gcagccacgg agaacttcga ggcggtgacc cggctcggtg
     4141 tgatcctatc gcccaccaac aaggatgcca cggtcttccc cgagccgttt gacggcatgt
     4201 ggatcctgct ccaccggccg gtgaccgggg gccaggagca catctggtat gcctgctccc
     4261 ccgaattgac ccactggagc ctgccggggg tcttgctgcc ccaacggagc ggcccatggt
     4321 gggatggctt gcgggttgga gttggggccc cggccctgcg aaccgaccag gggtggctgc
     4381 tgatctacca tggagtcaag gatatggccg gccggccgat ctacaggctc gggcttgcct
     4441 tgctcgatct ggagaaccct cgaaaggtgc tcgcccgtgc gtccaggtgg gtgttcgcgc
     4501 ctgataccga ctacgaacag cgagggctgg tgcccggcgt cgtatacacc tgcggggcgc
     4561 tcctgcgggg tgatgaggtg tggatgtact acggcgcggc agacacggtg atcggcctgg
     4621 caatagccaa agtgtcggat cttctcgcct tcgtccatca acacgacttc ttgcatcaag
     4681 tgggtcgaga aaaagggacg gcatcgtgac gtcaccgcga ttcgcgggtg gttcacggcc
     4741 gacacgtcgt caacgtactc ccgtgaacat ggagggtgag aatggcagcg caccgcaagt
     4801 ctgggctcta caggccatcg ttggtcggtg tcttggcaac cggcctgctg gtgctcgtac
     4861 tcgccaagtg gcccacgtgg cgacgagacc ccaaccgtct cttccgagca atgtacaacc
     4921 tcgtcgcccc aatctacgat ctctgggcgg gagagaagag cgcgctggct cctctgcgtc
     4981 aacagctggc ggagatgatg gagttggagc ccggagacca ggtgctggag gtgggtgcag
     5041 gcacgggggc
//
LOCUS       DATALS010000180         6754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_242929, whole genome shotgun sequence.
ACCESSION   DATALS010000180 DATALS010000000
VERSION     DATALS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242929"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VHS06_03995"
     CDS             <1..1121
                     /locus_tag="VHS06_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX2987314.1"
                     /translation="AAGHGCGHNLLGVGSLGAAVALKEAIRAEGLHATVRYYGCPAEE
                     TAIGKVFMAREGAFDDLDAALSWHPGGVNMATGCSSLALNSVKFAFHGRSAHASAAPH
                     MGISALDAVELMNVGVNYLREHVTPEARIHYVITDGGSEPNVVPANAEAWYYIRAPHR
                     HDVEEIYQRILKIAEGAALMTGAGLEVKFQAGLYDILPNPVLIELLERSMVEVGVPSF
                     TPEEMEFAKGIEASFSSGMKEAAIQGHHLPAEMATVILHDQVMPCSDRGTAMWASTDV
                     GDVSWIVPTGQVNAASYVFGTPAHSWQAVAAAGMSIGQKSMLTAARSLALGGYRLVTQ
                     PELLSQAWEAFDKARGGHKYVSPVPADVAPPDPHLDGH"
     gene            1220..2056
                     /locus_tag="VHS06_04000"
     CDS             1220..2056
                     /locus_tag="VHS06_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2987315.1"
                     /translation="MEKSMGDLSGKVAWVTGSSRGIGRSVAAYLAGMGATVIVHGTTP
                     TSTRAFNEADSLEAVAKSIAADTGAEVMAVHGDLTEEASVKRIVGEIRAKYGRIDILI
                     NCAGGDIGAAGTGAPAGGYPRGNDALNISFDDIKAVLNRNLLTCILVCREVVPEMQER
                     RDGCVVNFGSIAGHGPKTDGVIYAAAKAGVAQYSRDLAWQMRPFNVRVNVVAPGPIMS
                     ARFMVSRPIDQKRVEGGATLDRYGHPEEIATVVGFLVSDAASYISGQVLRVDGATQCW
                     SA"
     gene            complement(2133..3152)
                     /locus_tag="VHS06_04005"
     CDS             complement(2133..3152)
                     /locus_tag="VHS06_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifH"
                     /protein_id="HEX2987316.1"
                     /translation="MDANWKALLTGDPLPWLLEDDTPAVRHATLRTLMGKADDDPEVR
                     SALATAMSQGPIATILEAQNPDGYWAKPGAGYGPKYQGTVWQLVFLDQLSADPHAPAV
                     KLACEYVLAHCQTSTGAFGCSAARTLAPPPPSAAIHCLHGNLLRALLGFGYFDDVRVR
                     QAIDWQARSITGDGFGRYYQSSTSGPDCACAANGKKPCAWGAVKALLALARIPAEKRD
                     EPVASAIDRVVELLLSRDPAIADYPTTFEGQRPSGAWFQLGFPLGYVTDVLQNLEALC
                     EAGFAGDPRLRNAVDWLLSKQDRQGRWSNRHAYNGKTWGDIERQGAPSKWVTLRACHV
                     LQMMA"
     gene            3359..4444
                     /locus_tag="VHS06_04010"
     CDS             3359..4444
                     /locus_tag="VHS06_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2987317.1"
                     /translation="MKSYAIPMVPGPTSVPREVLAAYQFDYGSSDLEEEFCSLYGRVQ
                     DQLRQIMGTRNKMAIMTGEGMLALWAALKSCLSPGDRVLSVATGLFGFGVGDMAASLG
                     CEVETVGFDYDAIADAGRVEEAIARFRPKMVTMVHCETPSGTLNPVGEVGRLIEKHEV
                     PLFYVDAVSSAAGAPLRTDEWNIDLCLVGSQKALSAPSAISIVSISPRAWDVIGEVGY
                     QGYDALAPWEVALESRYFPYTPYWHGMAAMEVACQRILDEGMEKVIARHELVAAQCRH
                     GVMDLGLELYPREEAFCAPTVTAVRVPESIGWEEFDGRLRSRGVVVGGNYGVLAGKVF
                     RLGHMGTQARSELMAHTLDAIREVLKS"
     gene            complement(4536..5096)
                     /locus_tag="VHS06_04015"
     CDS             complement(4536..5096)
                     /locus_tag="VHS06_04015"
                     /inference="COORDINATES: protein motif:HMM:NF025601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HEX2987318.1"
                     /translation="MSERSTRFDKMAKESSDPEGLRDEAYVPQDVEEPRELSATSAED
                     SPTPTVSSMSSEMGGTGKPEGPTPDIPGSEGTEWQGRNEEIPVHTTESGGSTTGQKGR
                     TWTEQIEMAGGEVVNRIGQIVAQGNVRRLIFRRSDGRVLLEVPLTAGVAVTGVAVLAA
                     PVLAALSAMAALVAQVKVEIVRSSEE"
     gene            5370..6095
                     /locus_tag="VHS06_04020"
     CDS             5370..6095
                     /locus_tag="VHS06_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX2987319.1"
                     /translation="MNSGVESKSSEGSGVALRAVDLSRQVGSKILVDGVSVDVGHGDV
                     LAIVGPSGAGKSSFLRLLNRLDEPTGGTVMVDGKDYRSMAPRSLRRMLGMVLQSPFLF
                     SGTVSYNLRYGPAQWGESLSGEREEHLLERVGLAGYGARDISTLSGGEAQRVSLARTL
                     ANSPRVLLLDEPTSALDEGSKEGVEALIRDILAEQGLTCVMVTHDMGQAARMANRVML
                     MESGRMVRTGTVAEVLNASPIVH"
     gene            6073..>6754
                     /locus_tag="VHS06_04025"
     CDS             6073..>6754
                     /locus_tag="VHS06_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2987320.1"
                     /translation="MLPRLFTDPLSLGFAQASVAAAMALAVMWLSRSWAIHVERETVV
                     ALARGIVQVVLVGSVLVVLLQGPLWTSAIVLSVMVGAASATAARRAKDIPGALWASSW
                     GMAIGSGVVILLGAIIGVIDVRVASIVPVGSMIIANGMNAAALALDRFRAEVVSQTGR
                     IEASLALGADPAVSVAPYLQSAMHASMTDRTIAEVQSGPCNSTTSTEPTSTTCTMPLA
                     RATTVSRST"
BASE COUNT         1192 a         1929 c         2291 g         1342 t
ORIGIN      
        1 gggcagcggg acatggctgc ggacacaacc tgttgggagt tgggagcctc ggagccgcgg
       61 ttgcgctcaa ggaagcgatt cgggcagagg gactgcatgc gaccgtcagg tactacggct
      121 gccccgcgga ggagaccgcc attggcaagg tgttcatggc gcgggagggg gccttcgacg
      181 acctggatgc ggcgctgagc tggcatcctg gcggggtgaa catggccacc ggctgtagct
      241 ccctggccct caactcggtg aagttcgcct tccacggtcg cagcgcccat gcctccgctg
      301 ctcctcacat ggggataagt gcattggacg ccgttgagtt gatgaacgta ggcgtcaact
      361 atcttagaga gcacgtgact cccgaggcga ggatccacta cgtcatcacc gatggcggca
      421 gcgagccgaa cgtagtccct gccaacgccg aagcctggta ctacatcaga gcccctcaca
      481 ggcacgatgt ggaggagata taccagcgga tactgaagat agccgaaggc gcggcgctga
      541 tgaccggagc gggactggag gtcaagttcc aggccggact gtacgacatt cttcccaatc
      601 ccgtcctgat agagttgctg gagaggagca tggtcgaggt gggtgtgcca tccttcactc
      661 cggaggagat ggagttcgcg aaggggatcg aggcgagttt ctcgtccgga atgaaggagg
      721 cggccattca gggccaccac ctgcctgccg agatggctac tgtgatactc cacgaccagg
      781 tgatgccatg ttctgaccgt ggtacggcga tgtgggcttc tacggatgtt ggggacgtga
      841 gttggatcgt ccccacgggg caggtgaacg cggcgtccta cgtcttcggg actcctgccc
      901 actcctggca ggccgtcgct gctgcgggta tgagtatcgg gcagaagagc atgttgaccg
      961 cggcccgctc cctggctcta gggggctata ggctggttac ccaaccggag ttgctgtctc
     1021 aggcgtggga ggcatttgac aaggcgcgtg gtggccacaa gtatgtatcg cccgtaccgg
     1081 ccgatgtggc tccgcctgat cctcatctgg atgggcacta gatgctgcta tcagcgattg
     1141 aaacggacgg atggctaggg tacactcgcc gcgtctgtat gcctggctgt gatgccggga
     1201 gtgtgggagg agtagacgaa tggagaagag catgggcgat ctcagtggaa aggtggcatg
     1261 ggtcaccggt tcatcccgag gtatcggccg ctcggtagcc gcctatctgg caggcatggg
     1321 agcgaccgtc atagtacatg gcaccacccc cacctctact agagccttca acgaggcgga
     1381 ctcgctcgag gccgtagcca aaagcatcgc tgctgacacc ggtgccgaag tgatggcggt
     1441 tcacggcgac ctgactgaag aggcatccgt gaagaggatc gtcggtgaga tccgcgcgaa
     1501 gtacggccgg atagatatcc ttatcaattg cgccggtgga gacatcgggg cggcgggcac
     1561 tggggcaccg gctggagggt atccgcgggg gaacgacgcc ctcaacatct ccttcgacga
     1621 catcaaggcg gttctgaatc gcaacctctt gacatgcatt ctcgtatgcc gagaggtcgt
     1681 tcccgagatg caggagcgca gagatggttg cgtggtgaac tttgggagca tcgccggcca
     1741 tggtccgaag accgacggag tcatctatgc cgccgcgaaa gcaggagtgg ctcagtactc
     1801 ccgcgatctg gcttggcaga tgaggccgtt caacgtccga gtgaacgtgg tggcccccgg
     1861 cccgatcatg tccgctcgtt tcatggtgag ccggccgata gatcagaagc gtgtcgaagg
     1921 tggtgcgacg ctggaccggt acggccatcc cgaggagatt gcgacggtgg tgggcttcct
     1981 ggtctccgac gcggcttctt acatcagcgg gcaggttctc cgcgtcgacg gcgcaaccca
     2041 gtgctggtcg gcctagacgc gacgatcgga ctcagtggca ccacgagggc ggggggctgt
     2101 cgctccggca gttcccgccc tctcttttct tcttacgcca tcatctgaag gacgtggcag
     2161 gctcgcagcg tgacccactt gctgggggct ccctgccgtt cgatgtctcc ccaggtcttt
     2221 ccgttgtagg cgtggcggtt agaccaccgg ccctgtcggt cctgtttcga caacagccag
     2281 tcgaccgcgt tgcgcaatct gggatcgccg gcgaaacccg cttcgcatag cgcctccagg
     2341 ttttgcagta cgtcggtcac gtatcccaga ggaaagccca gttggaacca ggcgccgctc
     2401 ggtctctggc cctcaaaggt agtcgggtag tccgcgatcg ccggatcgcg gctgagaaga
     2461 agttccacaa cccgatcgat cgcgcttgcg accggctcgt cgcgcttctc cgccggtatc
     2521 ctcgcgaggg cgaggagcgc cttcaccgcg ccccaggcac acggcttctt cccgttcgcc
     2581 gcacaggcac agtcggggcc agaggtagat gactgatagt agcggccaaa gccgtctcca
     2641 gtgatggaac gagcctgcca gtctatcgcc tgtcggaccc tgacgtcgtc gaagtagccg
     2701 aagccgagca gagctcttag caggttaccg tgaagacagt ggatcgcggc ggatggaggc
     2761 ggcggggcga gtgttctggc cgcggagcat ccgaaggctc cggtactggt ctggcagtgg
     2821 gccagaacgt actcgcaggc gagcttgacc gcaggagcgt ggggatcggc actcagttga
     2881 tcgaggaaga caagttgcca gaccgttccc tggtacttgg gaccgtagcc cgcgccgggc
     2941 ttggcccagt acccatcggg attttgggct tcgaggattg tcgcgatcgg cccctggctc
     3001 atggctgtgg ccagggcgga gcgtacctcc ggatcgtcgt cagcctttcc catcagggtg
     3061 cgcagggttg catgtctgac tgccggggtg tcgtcttcca gaagccacgg cagcgggtct
     3121 ccggtgagca gcgccttcca attggcatcc atcgttcttc tccgtttcac cctttcccag
     3181 cggagttcct ctgctcacat gatagctcag gagcgcgctg ttgccatacc cccgtttgcg
     3241 ctgtagggcg ctgtggggga tacttcgggc atagagggag taatggatca ggagacgggc
     3301 ggcaaatggg gatctaccga tcgctccccg tcgcctgttc gcgaaagtga ggctgaaggt
     3361 gaagagctac gctattccca tggttccggg gcctacctcc gtgccgagag aggtgctggc
     3421 cgcgtaccag ttcgactacg gttcctccga tctggaggag gagttctgtt cgctgtacgg
     3481 acgcgtgcag gatcagttgc ggcagatcat gggcacccgg aacaagatgg ccatcatgac
     3541 cggcgagggg atgctcgcgc tatgggctgc gttgaagagt tgccttagcc ccggcgaccg
     3601 ggttctatcg gttgccacag ggctattcgg ttttggtgtt ggcgatatgg ctgcatctct
     3661 ggggtgtgag gtggagaccg tcgggttcga ctacgatgcc atcgcggatg ctggaagggt
     3721 ggaggaggcg atcgcacgct tcaggcccaa gatggtcacg atggtccact gcgagactcc
     3781 gtccggcaca ctgaaccctg tgggagaggt cggacggctg atcgagaagc atgaggtgcc
     3841 gctcttctac gtggacgcgg tttcgagcgc ggccggtgcc cctctgagga ccgacgagtg
     3901 gaatatagat ctgtgcctgg tcgggagcca gaaggcgttg tcggcgccct cggcgatctc
     3961 gatcgtttcc atcagtccaa gggcgtggga tgtgataggg gaggtgggct atcaggggta
     4021 cgacgccctg gctccctggg aggtggcact ggaaagccgc tacttcccgt acactcctta
     4081 ttggcacggg atggccgcca tggaggttgc ctgccagcgg atcctcgatg aagggatgga
     4141 gaaggtgatc gctcgccacg aactggtggc agcccagtgc aggcatggcg tgatggatct
     4201 gggcctagag ctgtacccac gagaggaggc tttctgcgct ccgacggtga cggcggtcag
     4261 ggtccctgag tccatcggtt gggaggagtt cgacggccgt cttcgatcgc gcggtgtggt
     4321 agttggaggc aactatggcg ttctcgcggg caaggtattc cgtctagggc acatgggaac
     4381 gcaggcgagg agcgaactaa tggcccatac tctggacgct atccgtgagg tgctgaagtc
     4441 gtagcgttcg cccgttagca gtgcggttgg agggcaggtc tgcgcctgcc ctccaacctg
     4501 attcgccggt accggggagc ggttcggaca ggtggctact cctcggacga cctcacgatc
     4561 tccaccttga cctgagcgac gagggcggcc atcgcgctga gggcggcaag caccggtgca
     4621 gcgagcaccg ccactccggt gaccgcgact ccagccgtga gcggcacttc gagaagaact
     4681 ctgccgtccg acctgcggaa tatcaggcgg cgaacgttcc cctgggcgac gatctggccg
     4741 atccggttga ccacctcgcc accagccatc tcgatctgct ccgtccaggt tcgccccttc
     4801 tgtccggttg tgctcccgcc tgactcagtc gtgtggaccg ggatttcctc attcctgccc
     4861 tgccactcgg tgccttccga gcccgggata tccggagtag gcccctccgg ctttcctgtg
     4921 ccacccatct ccgacgacat cgatgacacc gttggcgtag ggctatcctc cgctgaagtg
     4981 gcacttagtt ctctcggttc ctccacatct tgcggtacgt acgcctcgtc cctcagccct
     5041 tcgggatcgg agctttcctt ggccattttg tcgaaccggg tgcttctttc gctcatcctt
     5101 caccttcttt cgccgttgcc ggcctgtccg gcatcgtgac cgaagccccg tggtgctcgg
     5161 tcatcggtag cccctcgcac caccgaggct gctactcaac gatatatgag cagcagatgt
     5221 accagcgcta aatcaaggag caaatgttga ggaaatggcg ctccatcgga aggcgtgaac
     5281 tgcttcagtg atacaatcgt tctctgggat cagggctgaa gttcggcgca gcccgtgcgt
     5341 cgcgccgggg tggccagtgg aggtcgggag tgaatagtgg tgttgagtcg aagtcgagcg
     5401 aaggcagcgg cgtcgctctg cgcgccgtgg acctaagccg acaggtgggt agcaagattc
     5461 tcgtcgacgg agtatcggtg gacgttggcc acggagacgt gctggccata gtggggccga
     5521 gtggcgcggg gaagtcgtcg tttcttcgcc tcttgaacag gctggacgaa ccgacaggtg
     5581 gcaccgtcat ggtcgacggg aaggactatc ggagcatggc cccgaggagc ctcagaagga
     5641 tgctgggcat ggtccttcag tccccgtttc tgttctccgg aacggtgagc tacaacctcc
     5701 gctatgggcc tgctcagtgg ggcgagtcac tatcgggcga gagagaagag catctgctcg
     5761 agagggtggg actggcaggc tacggtgcca gggacatcag cactctgtcg ggcggcgagg
     5821 ctcagagagt ctcgctggct cgcaccctgg ccaactcccc gcgagtgctt ctgctggacg
     5881 agccgacctc tgcgctagac gagggatcga aagagggggt ggaagcacta atacgagata
     5941 tcctggcaga gcaggggttg acctgtgtga tggtcaccca cgacatgggc caagcagccc
     6001 ggatggcgaa ccgggtcatg ctgatggagt cgggccggat ggtgcgtacc ggtaccgttg
     6061 cggaggtgct gaatgcttcc ccgattgttc actgatccgc tatcgttggg gtttgcccag
     6121 gcatcggttg ccgctgcaat ggcgctggcg gtgatgtggc tgagccggtc gtgggctata
     6181 catgttgagc gggagacggt agttgccctg gcgaggggca tcgtgcaggt tgtgctggtt
     6241 ggctccgtgc tggtggtgct gttgcagggg ccgctctgga cttccgcgat cgtcctgtct
     6301 gtcatggtgg gggcagcatc cgcaacggca gcgcggcggg ccaaggatat cccaggtgcg
     6361 ctgtgggcct cctcctgggg gatggcgatt gggtcggggg tggtgattct tctgggggcg
     6421 atcatcggcg tcatcgacgt gagggtggcg tccatcgtgc cggtgggcag catgataatc
     6481 gccaatggga tgaacgccgc ggctctggca ctggatcgct tcagggctga ggtcgtatcg
     6541 cagacggggc gtatcgaggc gagtctagca cttggggctg atccggcggt gtcggttgcg
     6601 ccatatctcc aatccgcgat gcacgccagc atgacagaca ggacgatcgc ggaagtccag
     6661 agcggcccct gcaacagcac caccagcacg gagccaacca gcacaacctg cacgatgccc
     6721 ctcgccaggg caactaccgt ctcccgctca acat
//
LOCUS       DATALS010000181         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_242977, whole genome shotgun sequence.
ACCESSION   DATALS010000181 DATALS010000000
VERSION     DATALS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242977"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VHS06_04030"
     CDS             <1..302
                     /locus_tag="VHS06_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987321.1"
                     /translation="SLWDLEEAVILHSAYAGSTVHPTSRTPSSGIFFWYLAPAEVQPT
                     CRTGAWKDMELEMDEKSSTKDPGMPPDREMVSMDLPLLLLVLIVLLSPLLYDMAC"
     gene            366..1313
                     /locus_tag="VHS06_04035"
     CDS             366..1313
                     /locus_tag="VHS06_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2987322.1"
                     /translation="MKDPEALIRVEGLKKSYPGGVQALKGVSFQVHRGDRACILGPNG
                     AGKTTLIRLLGGVLSPDSGMAHLMGAQAGTPAFLDAKRSVGIVPEAPGMYQEIRVGEY
                     LQFVRDLYRRGSIGGVVGALELEPYLDRPMNTLSGGYQRRVVLAAALLPEPELLILDE
                     PTARLDPVASAQIRHYIRKVAEGRTLLLCTHNLAEAEELCDRVIILRQGTVLVQDELE
                     TLRSRFARHLDLEAVDGQDRMVQLLAEAGYRASTDGGRFRVVVSDYKKEVPALLSRLL
                     SEGIQLYGAHVQEPSLEEIFLRLMGEETELEAGDGEVRR"
     gene            1310..2050
                     /locus_tag="VHS06_04040"
     CDS             1310..2050
                     /locus_tag="VHS06_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2987323.1"
                     /translation="MSYELKILFAKEWKQAVRNRGALLTATLLPLFLLLIVPVAQAIL
                     ATQGLGTTGMNTGPLPPGLTDASPEAVFVQVTFPLFVVLGGLIVPSLAASHTVVSERE
                     KRTVDLLMALPVSLGQIVAGKVAAVVALALAITLPLVLIDFVLLSLLGLADLSLILFY
                     ALELLASLTFSVSSTVAIALLARDYRSANNLSGAFMGPMILLTMAFNLLLPWGLYRLA
                     LHAGLMLLVGFVTIWVTLKVVSLERYAL"
     gene            complement(2173..>2968)
                     /locus_tag="VHS06_04045"
     CDS             complement(2173..>2968)
                     /locus_tag="VHS06_04045"
                     /inference="COORDINATES: protein motif:HMM:NF027450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HEX2987324.1"
                     /translation="MAGKLRVIAATNAFGMGVDKHDVRFVIHRDVPASVEEYYQEVGR
                     AGRDGELALCSLIYRPGDLGRAAFFSGTGQLTLPEVRRARKGLLAHPEASWDEMEEAT
                     GLKEGDLARLVGFLVRDGVLEEKGGQIRMRVPDFDPERVSLQAEESRQAYEHSRLEMI
                     RGYAEAHDCRRRYLLNYFGQEYEPHRCGLCDNDIGEAEEAVEVDVEETAPSPFALGDR
                     VVHVDWGAGVVQRVAGDVVTVLFDKVGYKTLDIPIVEEQGLLKKAA"
BASE COUNT          511 a          921 c          904 g          632 t
ORIGIN      
        1 gctccctctg ggacctggaa gaagcagtca tactgcattc tgcgtatgcc ggctccaccg
       61 ttcatccgac gtcccgaact ccatcttctg gtatcttctt ctggtacctt gcccctgccg
      121 aagtgcagcc aacgtgccgg acaggcgcct ggaaagatat ggagctggag atggacgaga
      181 agtcgtcgac gaaagatcct ggaatgccac cagatcgcga aatggttagc atggatcttc
      241 ccctcttgtt gctggtgctc atcgttctgc tgtctccact cctgtacgat atggcgtgct
      301 gatgcacctg cttgcctcat gcgttttgct tgggaggaca gggcgatgag aggaggatcg
      361 agcggttgaa ggatcctgag gcactgatac gggtggaggg actcaagaag agctatcccg
      421 gcggggttca ggcgctgaag ggggtcagct ttcaggtgca ccgtggggac cgggcctgca
      481 tcctgggacc aaacggcgcg ggcaagacca cgctaattcg actgttgggc ggcgtcctct
      541 cccccgattc aggcatggct cacctgatgg gcgcccaggc cgggaccccc gccttcctgg
      601 atgccaagcg atcggtcggc atcgttcctg aggcccccgg tatgtatcag gagatcaggg
      661 ttggggaata tctgcagttc gtcagggatc tctaccgtcg cggctcaatc gggggtgtcg
      721 ttggcgctct ggaactggag ccctatctcg ataggccgat gaacaccctc tcaggcggct
      781 accagcgtcg ggtcgtcttg gcggcagcgc tgctgccgga gccggagttg ctcatactcg
      841 acgagcccac cgccaggctc gatccagtgg cctcggccca gatccgtcac tacatccgaa
      901 aggtcgccga gggtagaacc cttctactgt gcacccacaa cctggccgag gccgaggagc
      961 tgtgcgatcg ggtgatcatc ctgcggcagg gcacagtcct ggtgcaggac gaactggaga
     1021 cgctgaggag tcgcttcgcc cgccatttgg atctggaagc ggtggatgga caggatcgca
     1081 tggttcagct gcttgcggag gcaggctaca gggcctcgac cgacggtggt cgcttcaggg
     1141 tggtcgtgtc cgactacaag aaggaggtcc ccgcccttct ctcgagacta ctgagtgagg
     1201 gtatccagct ctatggggcc cacgtccagg agccatcact ggaggagata ttcctcagac
     1261 tgatgggcga ggaaaccgag ctggaagcgg gagatgggga ggtgcggcga tgagctacga
     1321 gttgaagatc ctgttcgcga aggagtggaa gcaggctgtg cggaaccgtg gagcactcct
     1381 caccgccaca cttttgcctc tcttcctgct gctcatagtt cctgttgcgc aggcaatcct
     1441 cgccactcag gggctcggga cgacgggaat gaacaccggt ccactaccac ctggattgac
     1501 cgacgcctcc cctgaggcag tcttcgtcca ggtcaccttc cctctcttcg tcgtgctggg
     1561 tggattgatc gttccctctc tggcggccag tcatactgtt gtctccgagc gggagaagcg
     1621 gaccgtggac ctgttgatgg cgttgcccgt atccctgggc cagatcgtcg cgggcaaggt
     1681 ggccgccgtc gtggccctcg ctctggcgat caccctgccg ctggtgctga tcgatttcgt
     1741 gctcctgtcg ctgctgggtc tggccgacct gtccctcatt ctcttctatg ccctggagtt
     1801 gctggcgtct ctgaccttct cggtgtcctc gaccgtggcg atagcgctgc tggcccgaga
     1861 ctacaggtcg gcgaacaacc tgagcggagc cttcatgggg ccgatgatcc tgctgacgat
     1921 ggccttcaat ctgttgctgc cctgggggct ctacaggctt gcgctgcacg ccgggctgat
     1981 gctgttggtg gggtttgtga ccatatgggt taccctgaag gtggtgagtc tggagcggta
     2041 cgccctctga cacgttgatc ccggttccag tggattagtc atcctgaacg gagtgaagga
     2101 tctctccggc aacgaggaga tccttcactc cgttcaggat gacacctgcc tcaggatcac
     2161 tgtgatctag gattaggcgg ccttcttcaa caggccctgc tcttcgacta tcgggatatc
     2221 cagcgtcttg tagccgacct tgtcgaagag caccgtcacg acatccccgg ccacccgttg
     2281 gactacccct gcaccccagt cgacgtgaac tacccggtcg cccagggcaa agggcgaagg
     2341 cgccgtttct tccacgtcga cttccactgc ttcctccgcc tcaccgatgt cgttgtcgca
     2401 caggccacag cgatggggct cgtactcctg cccaaagtag ttcagcaggt agcgacggcg
     2461 acaatcgtga gcctccgcgt agccgcgaat catctccaac cggctgtgct cgtatgcctg
     2521 tcggctctcc tccgcctgga gggagacacg ctccgggtcg aagtccggca ctcgcatccg
     2581 gatttgtcct cccttctcct ccaggacgcc atccctcact agaaaaccga ccagcctggc
     2641 gagatccccc tctttcaggc ccgtcgcctc ctccatctcg tcccaggatg cctcggggtg
     2701 agccagcagt ccctttcgag cacgcctcac ctcgggcagc gtgagctgcc ctgttccaga
     2761 gaagaaggcg gcacggccga ggtcgcccgg ccggtatatc agtgaacaga gggcaagttc
     2821 cccgtctctt cccgctcgac ccacctcctg gtagtattct tccacgctgg ccggtacgtc
     2881 gcggtgaatc acgaagcgca cgtcgtgctt gtcgaccccc atgccgaagg cgttggtagc
     2941 ggcgatgacc cgcagctttc ctgccatg
//
LOCUS       DATALS010000182         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_198912, whole genome shotgun sequence.
ACCESSION   DATALS010000182 DATALS010000000
VERSION     DATALS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198912"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VHS06_04050"
     CDS             complement(<1..984)
                     /locus_tag="VHS06_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987325.1"
                     /translation="MFRKVLLVLVLGIATLAAVGFVQRTNGLKVAAPVHGQDVGGKIA
                     YARAGALWIYSGGSAQQLTKGPQDRQDKRDTSPSFSPDGTEIVYARTDEGFSDLYKLS
                     LNNPAKTLALTSYRPDVQTGAQGYNKQALWALQPAWSPDGSKIAYLNDKGTEYPGLYL
                     MTAEGDNPHRIDFLDHSTQAVERPAWSPDGTKIAVANYVTNGAKGQIWVLNLDTGRWT
                     AVTNAPDGAYDPAWSPDGEWLAFTMRSGKANDIYVAPTDATKWTTEYPTPSQLTTDGN
                     SRSPAWSPDGKRLAFIGLQDNSFNLYAASVGQGTGGAPTLSSIQRLIQGGNL"
     gene            complement(1019..2224)
                     /locus_tag="VHS06_04055"
     CDS             complement(1019..2224)
                     /locus_tag="VHS06_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX2987326.1"
                     /translation="MTTSEGDVPGPKDGASLTPSGTAIASPLLLPSTDQTPGEWDRIV
                     RVAGGGLLQSWQWGEFKRQAGWEPVRLSLNRLGDVVGESASQTVTAAQVLFRAVPHLA
                     LPVSIAYVPRGPLDLTGALSSSPAGDVFWRDVHQEARRRGAIFLKVEPDIRLSDERRK
                     EEVDRTMAGLGFRPSGRLQPARTIVLDLHKSEEDLLKAMKPKTRYNLRLSERRGVRVR
                     RAESIGDLREFYSLLEVTGNRDEFGIHSLRYYEHLWKLFGPSGANSMLLLLADDPDDA
                     ERAAGPIAGLVVLQFGHEAIYMYGASADRGREHMPNYLLQWEAVRWARGQGCDVYDFW
                     GIPDPPDEEPGPNEAVSPTNTRSGLRGVYWFKKGFGGKEVEYPGAYDYVYNPLLYRLW
                     MRWRGSDLG"
     gene            complement(2211..3053)
                     /locus_tag="VHS06_04060"
     CDS             complement(2211..3053)
                     /locus_tag="VHS06_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX2987327.1"
                     /translation="MAHNVGVFVDVSNLFYSAKSAGVEVNYCGLLEQAVAGRDLIRAC
                     AYTGVDPENPNQRKFIDFLKSNGYKVVCKDIHKYEGGRIKANLDIEMAVDILLLSENL
                     DVVVIVSGDGDFVRLVEAVQMKGVRCEVIAFGISTSNELIEAADSFTEIGTLPGIFRN
                     GVPGPGVHIRSSQVQSESRQVYAYTSYSVSAGFGLPLERVSGEKERERLRAAERGAHG
                     GYLESRDDRNRAEAAPSTNGQTSAYNHATHDEFMLPDAEPCVDPHSIPVARLETDKTP
                     DDDE"
     gene            3717..3977
                     /locus_tag="VHS06_04065"
     CDS             3717..3977
                     /locus_tag="VHS06_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987328.1"
                     /translation="MPLEELTVGHKSPKVYYVHNGPLHLEWLAEGSDGRMYVFPAEPG
                     GWLRRNPYSGPSGDLRCVSPEKAQSIAWLVYGDIDTVTIASQ"
BASE COUNT          744 a         1233 c         1277 g          789 t
ORIGIN      
        1 gaggttgcca ccctgaatca accgctgaat cgaggagagt gtgggcgcgc cgccggtgcc
       61 ctggccgacg gaagcggcgt acaggttgaa cgagttgtcc tgcagcccga tgaaggccag
      121 gcgcttgcca tcgggcgacc aggcaggaga gcgcgagttg ccgtctgtag tcagctggct
      181 gggcgtcgga tattcggtgg tccacttcgt cgcatcagtg ggtgcaacgt agatgtcgtt
      241 ggccttgccg ctacgcatgg taaacgcgag ccactctccg tcgggcgacc aggcggggtc
      301 gtaagctcca tccggggcgt tagtgaccgc ggtccatcgt cccgtgtcga ggttcagcac
      361 ccagatctgc cctttagcgc cgttcgtcac atagttggcg acggcaatct tggtgccgtc
      421 gggcgaccac gcggggcgtt cgactgcctg ggtgctgtga tcgaggaagt cgatgcgatg
      481 agggttgtcg ccttcggcgg tcatgaggta cagtcccggg tattcggtgc ccttatcgtt
      541 gaggtaggcg atcttcgagc cgtcaggcga ccaggccggc tgcagtgccc agagtgcttg
      601 cttgttgtag ccttgggcgc cggtctgtac atccggacgg tagctggtca gcgcaagcgt
      661 cttggccggg ttgtttagac tgagtttgta gaggtcggag aagccctcgt cggtgcgggc
      721 gtagacgatc tcggtcccat caggcgagaa ggacgggctc gtatcgcgct tatcctggcg
      781 atcctggggt cctttggtca gctgctgtgc gctgccgccc gagtatatcc acaaggcgcc
      841 ggcgcgggcg taggcgatct tgcctccgac gtcctgacca tggacgggcg ccgcaacctt
      901 tagcccattc gtcctctgca cgaagccgac agccgcgagg gtagctatcc caaggaccag
      961 aacgagcaag acttttctaa acacgcaatt ctctcaattg gcggaacatg aaatcaggtc
     1021 agcccaggtc ggatccgcgc catcgcatcc acagcctgta gaggaggggg ttatatacgt
     1081 agtcatatgc ccccggatac tccacctctt tgccgccaaa gcctttcttg aaccagtata
     1141 caccgcgcag tcccgagcgt gtattcgttg gggaaacggc ctcgttggga ccgggttcct
     1201 cgtcgggcgg atcgggtatg ccccaaaagt catacacgtc gcagccctgt ccgcgagccc
     1261 agcgtacggc ctcccattgc agcaggtagt tgggcatgtg ctctcgcccg cggtcggcgg
     1321 aggcgccgta catgtagatc gcctcgtgcc cgaactgcaa gacaacaagc ccggcgatgg
     1381 ggccggccgc gcgctccgcg tcatcggggt cgtcggccag gaggagcagc atactattag
     1441 cgccggaggg gccgaagagc ttccacagat gctcgtagta ccgaagacta tgaatgccga
     1501 attcgtcgcg attgcccgtg acctcgagga gggagtagaa ctcccgcagg tcgccgattg
     1561 actcagctct gcgcacgcga acgccgcgcc tttccgacag gcgcagattg taccgcgttt
     1621 tgggcttcat cgctttgagg aggtcttcct cgctcttgtg gaggtcgaga acgatggtac
     1681 gcgcgggctg caatcgcccc gaagggcgga aaccgagccc tgccatcgtg cgatcgactt
     1741 cctccttgcg gcgctcgtcg ctcaggcgga tgtcaggctc gaccttgagg aagatcgcgc
     1801 ctcgccttcg cgcttcctgg tgcacgtcgc gccagaagac atcgccggcc ggcgatgaag
     1861 acaaagcacc ggtgagatcg aggggcccgc gcgggacgta ggcgatggat accggaaggg
     1921 cgagatgagg gaccgcgcga aagagcactt gcgcggccgt cacagtctgc gaggccgact
     1981 cgcccacgac atcaccgagc cggttcagcg agagtcgaac tggctcccac cctgcctgcc
     2041 tcttgaattc gccccactgc cacgactgga gcaagccgcc tcccgcgacg cggacaatcc
     2101 ggtcccactc gccgggagtc tgatctgtgg atggaagcag cagtgggctc gcgatggccg
     2161 tgccggaggg cgtcagcgag gccccgtcct tcgggcccgg cacatcgccc tcactcgtcg
     2221 tcatcaggcg tcttgtcggt ttcgaggcgg gcgaccggaa tcgagtgagg atcgacacaa
     2281 ggctcggcgt cgggaagcat gaactcgtca tgcgtagcgt ggttgtaggc cgacgtctgc
     2341 ccgttcgtcg aaggcgcagc ctcggcgcgg ttcctgtcgt cacgagactc aaggtagccg
     2401 ccgtgagcgc ctctttcggc agcgcgcaac cgctcgcgct ccttttcgcc cgagacgcgc
     2461 tccagcggca ggccaaagcc tgcgctcacg ctgtacgaag tataggcata tacctggcgg
     2521 gactcgctct ggacctggct gctgcgaata tgtacgcctg gacccggcac gccgttgcgg
     2581 aagatgccgg gtagggtgcc gatttcggtg aagctgtcgg cagcctcgat gagctcgttg
     2641 gaggtgctga tcccgaaggc gatcacctcg cagcgcacgc ccttcatctg gacggcctcg
     2701 accaaccgga cgaagtcgcc atcgccgctg actatgacaa cgacgtcaag gttttcggaa
     2761 agcaggagga tgtcgacggc catctctatg tcgagattgg ccttgatgcg ccctccctcg
     2821 tacttgtgta tgtctttgca gactaccttg tatccgttgg acttcaggaa gtcgatgaac
     2881 ttgcgctggt tgggattctc gggatcgacg ccagtgtagg cgcaggcgcg gatgagatcg
     2941 cgcccggcta cggcctgctc cagcagcccg caataattga cttccacccc cgccgatttg
     3001 gccgaataga acagattcga tacatctacg aagaccccca cgttgtgggc catactatta
     3061 tctccattag agtgatttat gtacggtctc ttagccgaat atgatgtcct ggaaattata
     3121 ccttaaaagc cgccagtgta aactggcgag ctcagacttt gcgcggagcg ggcctcgcag
     3181 cgatggcgaa ccacggcgcc accctggccg gaggattccg cagcttgaca caggccttcg
     3241 gcctttcagc ctggatgttt cttgccaatc gggttataat tggcgaacca ggcgagcgag
     3301 gtcttcgaac tctcggcctc actacctcga acacaatcaa acgtcgcaga tcgccgctcc
     3361 ccgggatcgc ttccggacca ttcaaaggcc gacactctct cggctggagc aatccccatt
     3421 accggccggc ccgcctgccc ttttggctgc aggaaccgcc gcccactccc accaaacgcc
     3481 cggccaacta ctttcgctgt aggaggcctg gtcgccgata cccccaccgg atgcctggta
     3541 tgtagaaggg cccgggtaaa cggctcgccg tttcccggaa cgcggtcgcc ggattgcacc
     3601 ggggccgtat cggacgagat caggcgcaag tagaattgcc gggggtcgag ttgatgcgtg
     3661 accgatcatc agcgtctaac ttgagggaac tgcctcggcg ggaaagggga cgagcaatgc
     3721 cgctcgagga attgaccgtc ggacacaagt ctccaaaagt gtactatgtg cacaacgggc
     3781 ctctccatct agagtggctg gctgagggta gtgatggccg catgtatgtc ttccccgccg
     3841 aaccgggcgg ctggctcagg cggaatccct attccggccc gagcggcgac ctgcgatgcg
     3901 tgtcgcccga aaaagcccag tctatcgcgt ggctggtata cggagatatc gacactgtga
     3961 ccatcgccag ccagtaaccg gtggagtcgt ccctcgtggc tgcccggatc ggcattgatc
     4021 cgggcagtcg cgattctcag agg
//
LOCUS       DATALS010000183         2598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_152891, whole genome shotgun sequence.
ACCESSION   DATALS010000183 DATALS010000000
VERSION     DATALS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152891"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VHS06_04070"
     CDS             <1..902
                     /locus_tag="VHS06_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2987329.1"
                     /translation="VAETRGHRVTELLPAIGEAVNGTIGSRNEGETEFELGCGRSAQQ
                     YRMNVTPLRGKHGEELGHLLLLHDITSERRTQARLLEQERVVATLQERERLARELHDG
                     VGQVLGYLSLQTHAVRKRLQEGEGEKVDSQLLRLATVAQNAHGEVRESIFALTAASSA
                     NWSFLSTLRRYLNEFQANYGLDAELTVADGVDESLFDSHSAVQLLRVVQEAVTNARKH
                     AGACTVRVIVDARGGHACIKICDDGCGFDADGLGHDRDGHFGLAFMRERMAHIGGSVE
                     IESRPGGGTQVTLDAPVRNGDGR"
     gene            906..1631
                     /locus_tag="VHS06_04075"
     CDS             906..1631
                     /locus_tag="VHS06_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2987330.1"
                     /translation="MRVLLADDHLLLLEGLCNLLEAHGIDVVGVAKNGWEAADRAKSL
                     RPDLILMDIRMPGCDGLAATRLIKSEMPEAKIVILTTSTESDDLFEAVKSGAMGYLLK
                     SMDGDALIEALRDAEEDVPPFAPGFAAKLLAEFARQAKSTVDPPPSPPTEATAVRTSA
                     TASGLGTWPPDEPLTARQIEVLTLVAQGLAYKQIAASISLSPRTVKYHMTEIMRKLHL
                     QNRAQVLAYAGALARSRGSDRPR"
     gene            1808..2161
                     /locus_tag="VHS06_04080"
     CDS             1808..2161
                     /locus_tag="VHS06_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987331.1"
                     /translation="MGKNTRVLLYGNSVILASVRATLHHEGTFGTVLVAPPLASAEEL
                     EAMAPDVILFDLEKDRPDAVFSLLESRPDIILLGMSPDGNLVRLWSGRQFRELSSKDL
                     ENVIEEELAMRSATL"
     gene            2282..>2598
                     /locus_tag="VHS06_04085"
     CDS             2282..>2598
                     /locus_tag="VHS06_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987332.1"
                     /translation="MKRSVLVVVLTLLLVLAFCTQALAASAPYTGVALYCGSATLTDS
                     SKVTSPLVGGQPSAATYVNGVLKTSKSASLSGTTLVVNSATTPVPLLGQFMPADLVAS
                     LTQA"
BASE COUNT          488 a          830 c          836 g          444 t
ORIGIN      
        1 gtgtcgccga gacgagaggg cacagggtaa cagagttgct gcctgccata ggcgaggccg
       61 tgaacggaac cataggctcg aggaacgagg gggagactga gttcgagttg ggctgcggcc
      121 gatcagcaca gcagtaccgc atgaacgtga ccccgttgcg tggcaagcac ggggaggagc
      181 tcggtcacct cctgcttcta catgacatca ccagcgagag gcgaacgcag gcgcggctac
      241 tcgaacagga acgggtcgtt gctacgctgc aggagcgcga acgattggcg cgagaactgc
      301 acgacggcgt gggccaggtc ctcggctacc tgagcctgca gacccacgcc gtccgcaaac
      361 ggttgcagga aggcgaaggc gagaaggtgg actcccaact cttgcgcttg gcgactgtag
      421 cccagaacgc acatggcgaa gtgagggagt ccatctttgc cctgacagcg gcgtcctctg
      481 ccaactggtc ctttctctcc acccttcggc gatacctgaa cgaattccaa gccaactacg
      541 gcctggacgc tgagctgaca gtggcagacg gcgtggacga gagcttgttc gactcacaca
      601 gcgccgtgca gctgctcagg gttgtccaag aggcggtcac gaacgctcgt aagcacgcgg
      661 gcgcctgcac ggtgcgggtc atcgtggacg ctcgtggagg acatgcctgc atcaagatat
      721 gcgacgatgg ttgcggcttc gacgccgacg gactcgggca cgaccgagac gggcacttcg
      781 gactggcgtt catgcgcgag cggatggcgc acattggcgg aagcgtcgag atcgagtcgc
      841 gccctggtgg aggaacccag gtgacgctgg acgctccagt ccggaatggt gacgggaggt
      901 aggggatgag agtccttctg gctgacgacc acctcctgct cctcgaaggc ctgtgcaacc
      961 tgttggaggc ccacggcatc gacgtcgtcg gcgttgccaa gaacggatgg gaagcggcgg
     1021 accgggccaa gtcccttcgg ccggacttga tcctcatgga catccggatg ccgggttgcg
     1081 atggcctggc ggcgacgcga ctcatcaagt ccgagatgcc cgaggcgaag atcgtcatcc
     1141 tcacgacgtc gacggaaagc gacgacctct tcgaggcagt caagtcggga gccatgggct
     1201 acctgttgaa aagcatggac ggagacgcct tgatcgaggc gcttcgtgac gccgaggagg
     1261 acgttcctcc tttcgcgccc ggtttcgccg ccaagctgct cgccgagttc gcacggcagg
     1321 cgaagtccac agtggacccg cccccgtcgc cacctacgga agcgacggca gtccgcacca
     1381 gtgccaccgc atcggggctc ggtacctggc ccccggacga gccgctgacg gcacggcaga
     1441 tcgaggtgct cacgctggtc gcccaaggcc tggcgtacaa gcagatcgca gcctcgatct
     1501 ctctgagccc gcgtaccgtc aagtaccaca tgacggagat catgcggaag ctgcatctgc
     1561 agaaccgagc gcaggtcctg gcctacgccg gtgctctggc ccgcagccgc gggagtgacc
     1621 gcccgaggta gctccggccg gcctccccgc ctccgcctca ccctcgcact cacccaccga
     1681 ctgtcccccg ggacagtcgt gcccctcgtc cggactgtcc cctgatggtt cccagggaca
     1741 ttgctcgctc cgtcgcttct tttcagactt tctccaaggc ggagctcatc gcgacaaggg
     1801 tggtgggatg ggaaagaaca cgcgcgtcct tctgtacggc aactcggtga tcctcgcgag
     1861 tgtccgcgcc acgcttcacc acgagggcac cttcgggacc gtcctcgtcg cccctcccct
     1921 ggccagtgcg gaggagctcg aggcgatggc tccggacgtc atcctcttcg acttggagaa
     1981 ggaccgcccg gacgcggtct tctctctgct cgaatcacgc ccggacatca tcctccttgg
     2041 catgagcccg gacgggaacc tcgtccggct ctggtccggc cggcaattcc gggagctgtc
     2101 gagcaaggac ctcgagaacg tcatcgagga agagctggcc atgcggagcg ccacgctatg
     2161 aacgaatcgc actaacgaag aacgtcgctc gaaagggagg gtggatagtg gccaggcatc
     2221 gtccgtaggt cggctcctgc cgcacgcgtc caagcaagaa agatttaggg aggtctttct
     2281 gatgaaacgc agtgtcctag tcgttgtgtt gactctcctg ctggtgctgg ccttttgtac
     2341 ccaggcactg gcggcgagcg ccccgtacac cggcgttgcc ctctattgcg gcagcgccac
     2401 tctcaccgac tcgagcaagg tcacgagccc gctggtcggc ggccagccct cggccgctac
     2461 gtacgtgaac ggagtactga agacaagtaa atcggcgagt ctcagtggca ccactctggt
     2521 agtgaactca gcgaccactc cggtccccct gctcgggcag ttcatgcctg ctgacctggt
     2581 ggcctcgctg acccaggc
//
LOCUS       DATALS010000184         4570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_375821, whole genome shotgun sequence.
ACCESSION   DATALS010000184 DATALS010000000
VERSION     DATALS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375821"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="VHS06_04090"
     CDS             complement(<1..1385)
                     /locus_tag="VHS06_04090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2987333.1"
                     /translation="MGSLLFTARAEGRWPYLLAAAQAHIATLSIAAVTLGAFLLRLNG
                     LASKSVWYDEAFVVALARMSLPDMVRGLIELDPHPPLYYTFMHFWLLLGTDPFTLRLP
                     SAVFSTLSIPLLYQIGARLVGRKVGLGAAVLVAVSSFHVQWAQEARMYALLGLLCLGS
                     LFFLLRALDHGGLGNWAAHSVLTAACMYTQLNAVFFLAAQALGVVTLIATGQCDKSKL
                     RPWFSSQAVAAALFLPWLPAFVAQNETYREPNLAWTSVRGVEGVLFQHAYGQLPYWRI
                     TMRLAVELKDLLVVGALAVSVLGAWMLRRGGRGTLLLWLSLGTVGLLAAMGCWKGILL
                     GKTIIAASFAWFILIVAGLGALKRQEVVAVGLAALLLLNCLGLAKYYVAGSEEDWKAA
                     AGYLSSQVKPGEVAFVDCSAGLLPLDYQLEGRKERFELHGVPFKPWAVEPPVLTEEDY
                     RQVDQVASGRSG"
     gene            complement(1530..2303)
                     /locus_tag="VHS06_04095"
     CDS             complement(1530..2303)
                     /locus_tag="VHS06_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEX2987334.1"
                     /translation="MADECEFSLVIPAFNEAHRIVPSLERLTEYLGQSGVSHEITVVD
                     DGSTDDTLALCTRYSSEHPAMRVMGYPVNRGKGYAVKMGVLHSSGRLVAFGDADLPVP
                     PEDFARFIGALGRGSYDMAIGSRYLPSSSWDTSANRKLMGTAFRLAVQLIVGTRVSDT
                     QFGFKLFTSRAASELFSSLTIEGFAFDAEIVRLAHRRGYRIVELPVRGESAPGSTVRP
                     FHDAVAMLLDLLSIRLRRKAARRLRGETGRFPGVPMYRA"
     gene            complement(2325..3980)
                     /locus_tag="VHS06_04100"
     CDS             complement(2325..3980)
                     /locus_tag="VHS06_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987335.1"
                     /translation="MVAIPRSRWQDKEDAWGYRRDAAAVAAILVGSVALVVFFLLQEM
                     RLAGVLGMPLDDAWIHFRIAENLATGQGFSYNPGVPTSASTSPAWTALLALAYLVTGQ
                     FVMPALLMGVGCYLLSSIAVYYLALQVKSDRRIALVAALLCAATGRWIWAAMSGMETA
                     MFTGLILWGLLLHCRGSAKGRMALLGAGLVAVSTLLRPEGYLFLGLLILDRALVALLA
                     RPFSLRDAVQRLVPVAVSGPVLLLAAAIVARLAYTLVTGGGVAGNTFLAQSLPQGDGP
                     YTGPRLLPDVWYLRSAVDSLRTDDFLLGMLITVGLLSWFRTGLIERRVRRLTFLSLIW
                     FVGLPLANSFMAPNLRHHERYLMPLIPFAALLGSFGVDFAANQVASALPRVRSWILGR
                     WLTAVNLFLLLSIVCLGDALFEARRWSTQYAGDVRSIQAINVHLGEWLRDNTPSDAYI
                     AMNDIGAIAFISDRRILDTVGIAEPGILPYISQRGREGVMQYVRERRPDYLVIWPEWY
                     PEMASMRDVFEPIYSARVDRPLTATRESMLGGSEMVVYRAHWR"
     gene            complement(4179..>4570)
                     /locus_tag="VHS06_04105"
     CDS             complement(4179..>4570)
                     /locus_tag="VHS06_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987336.1"
                     /translation="RMYTLPIKEEWQEATSYVASRAEPGELVLFVATDAQFPFDYYLE
                     GEGIDLERRGLPVDVFTVGPLEPEVRPEDLGRVDRLVAGRDSFWLIESHEEFADPEGR
                     VRRHSDDRYRQMERREFVGIVVSRYGT"
BASE COUNT          993 a         1613 c         1219 g          745 t
ORIGIN      
        1 ccggacctgc cactggcgac ctggtcgacc tgtctgtagt cctcctccgt caacacggga
       61 ggctcaaccg cccatggctt gaagggcacg ccgtgaagct caaacctctc cttgcgccct
      121 tcaagttggt agtcgagcgg cagcagcccg gcgctgcagt cgacaaatgc cacctccccc
      181 ggcttcactt ggctgctcag atacccggcc gccgccttcc aatcctcctc ggaaccggca
      241 acgtagtact tggcaagccc caggcagttc agcagcaaca gggctgccaa ccccaccgcc
      301 acaacctctt gccgtttcag agcgcccagt ccggcgacga tcaagatgaa ccacgcaaat
      361 gaggccgcaa tgattgtctt gccaagcagt atccccttcc agcagcccat cgccgctagc
      421 aaccccaccg taccaagcga tagccacagc agcagcgtcc cccgaccccc gcgcctgagc
      481 atccatgccc ccagcaccga gactgcaagc gcccccacca ccagaaggtc cttcaattcg
      541 acggcaagtc gcatagtgat gcgccagtac gggagctgcc cgtaggcgtg ttgaaacagg
      601 accccctcca caccacggac cgaagtccag gcaaggttgg gctctctgta ggtttcgttc
      661 tgggcaacga aggccggcag ccagggcaag aacagcgcgg ccgcaactgc ctgacttgag
      721 aaccagggtc gaagcttaga tttgtcacac tgcccggtgg cgatcagcgt caccacccct
      781 agcgcctggg ctgcgagaaa gaagaccgcg ttgagttgtg tgtacatgca ggcggccgtc
      841 agcaccgagt gcgccgccca gttgccgagg cccccatggt cgagagccct cagcagaaag
      901 aagagtgagc caaggcacag cagcccgagc aaggcgtaca tccgggcctc ctgcgcccac
      961 tgcacgtgga aggaggagac ggccaccaat accgccgccc ccaggccaac cttccttccc
     1021 accaaccgag ctccgatttg atacaggaga ggtatcgata gggtggagaa gaccgctgag
     1081 gggagccgca gggtgaacgg gtcggtaccc agcagcagcc agaagtgcat gaacgtgtag
     1141 tagagaggtg ggtggggatc gagctcgatt agaccgcgca ccatgtccgg aagagacatt
     1201 cgcgcgaggg caacgacaaa cgcctcgtcg taccacacac ttttcgaagc caggccattc
     1261 aatcggagaa gaaacgcacc cagcgtgaca gcggcgatcg atagtgtcgc gatatgtgct
     1321 tgggccgcgg ccaacagata tggccaacgc ccctccgccc gtgccgtgaa caacaaggat
     1381 cccactatcg gttttctccg ctgtaggccg cagtcatcgg ccttctgaca gtcttctgac
     1441 atgcggccag tctaggaaag cggagttaac ctcaggtgaa ttgctgagtt gcaggctctt
     1501 tcatttctgt caccgtcatc catgcaccac tacgcacggt acatcggcac ccctggaaat
     1561 ctccccgtct ccccgcgcag tctccgagcc gccttccgcc gcaaccttat tgagagaaga
     1621 tccaacagca tcgccacagc atcgtgaaac ggccgcacgg tgcttcccgg ggcactctcc
     1681 cctcgaacag gcagctcgac tatccgatag ccgcggcgat gcgccagtcg cactatctcg
     1741 gcgtcgaatg caaacccctc gatggtcaag ctcgagaaca gctcgctggc ggccctggaa
     1801 gtgaacagct tgaagccgaa ctgggtgtcg ctcacccggg tgccgacgat caactgcacg
     1861 gccagccgga acgcagtccc catcagcttt ctattggccg acgtatccca cgatgaagag
     1921 gggaggtatc gagacccgat agccatgtcg taagagcctc tccccaacgc cccgataaac
     1981 ctcgcaaaat cctccggtgg aaccggcaaa tccgcgtcgc caaacgccac caaacgccca
     2041 ctggagtgca gcacgcccat cttcacagcg tagcccttcc cccgattgac ggggtagccc
     2101 atcaccctca tggccggatg ctcagatgag taccgagtgc acagtgccaa cgtatcatcg
     2161 gtactgccat catccaccac ggttatctcg tggctaaccc cgctctgtcc caggtattcg
     2221 gtcagcctct cgagggaagg gacaatccgg tgcgcctcgt tgaaagccgg tatgaccagg
     2281 gagaactcgc actcatcagc catcccaaga atcctttccc ccatttatcg ccagtgtgcg
     2341 cggtagacca ccatctcgct tccgcccaac atcgactcac gagtcgccgt gagtggcctg
     2401 tcgaccctcg cagagtagat cggctcgaaa acatccctca tcgaggccat ctcggggtac
     2461 cactcgggcc atatcaccag atagtccggc cgcctctccc tcacatactg cattaccccc
     2521 tctcgacccc gctggctgat gtacggcagt atgccgggct cggcaatgcc gaccgtgtcc
     2581 agaatccgcc ggtccgagat gaatgcaatc gcccctatat cgttcatggc aatgtaggcg
     2641 tcgctggggg tgttgtccct cagccattcc ccaagatgta cgttgatggc ctggatactt
     2701 cggacatcgc ccgcgtactg cgtggaccac cgtcgagcct caaacagggc atccccgagg
     2761 cacactatgc taagcagcag gaacaggttg acagccgtga gccacctgcc aagtatccag
     2821 ctacggactc gaggcagtgc cgaagccacc tgattcgcgg caaagtcaac gccaaagctg
     2881 cccaacagcg ctgcgaacgg tatgagcggc atcaagtagc gctcatgatg ccgcagattg
     2941 ggcgccatga atgagttcgc tagcgggagg ccaacgaacc agattaagct cagaaacgtc
     3001 agtcgccgga cccgccgctc gatgaggccc gtccgaaacc atgacagcaa acccactgtg
     3061 atcagcatgc ccagcaggaa gtcatctgtg cgcaggctgt ccacagcaga tcgcaggtac
     3121 cagacgtccg gcagcagcct cggtcccgtg tacggtccat caccctgtgg aaggctctga
     3181 gccaggaagg tgttcccagc cacaccgccg cccgttacca gcgtgtacgc caaccttgcc
     3241 acgattgcgg ccgccaacag cagcaccggg ccggagacgg ccaccggcac cagccgttgg
     3301 accgcatcgc gcagcgaaaa cggccgagcc aacagcgcca caagcgccct atcgaggatc
     3361 agcaacccga ggaagagata cccctccggc ctgagcagcg tcgacacggc cacaaggccg
     3421 gcgcccagca gcgccatccg gcccttcgca ctaccccgac agtggagcag gagtccccac
     3481 aagatgagac cggtgaacat ggccgtctcc atccccgaca tcgccgccca gatccaccta
     3541 ccggtcgccg cgcagagcag agcagccacc agcgcaatcc tacggtcgga cttcacctgc
     3601 aaggccaggt agtacaccgc tatggatgag agcaaatagc agccgacgcc catcaggagc
     3661 gccggcatca cgaactgtcc ggtcaccaga taggccagcg caagcagcgc cgtccaggcc
     3721 gggctcgtgg aagcggaagt gggcactccg gggttgtaag agaagccctg tccagtcgcc
     3781 aggttctccg ctatccggaa atggatccag gcatcatcca gcggcatgcc cagcacaccg
     3841 gccagcctca tctcctgaag aaggaagaag accaccaggg ccaccgagcc gaccaggatt
     3901 gccgcaactg cggccgcatc ccttctatag ccccaagcat cctccttgtc ttgccatcta
     3961 cttctcggaa tcgcaaccaa ctcccctgct cctctcgcct actactccgc caatccagcc
     4021 cctgtcatga ttctccccct ctccctccgc aacccgtcca ctccctctcc cactgcggtg
     4081 ggcgagggtc ggggtgaggg ccgcccccgg tcctcccccc ctcccacacg gagaaggggc
     4141 ccggggggtg gcgtccgccc tctatgggag acagcgcgct acgtcccata ccgcgacacc
     4201 actatcccca caaattccct ccgctccatc tgtcgatacc tgtcatcgga gtggcgccgc
     4261 acccggccct ccgggtcggc gaactcctca tggctctcga tcaaccagaa cgaatcgcgc
     4321 cctgccacca accgatccac ccggcccaga tcctccggcc tcacctctgg ctccaacggc
     4381 cccacagtga acacatccac gggaagccct cgccgttcga ggtcgatccc ctcgccttcc
     4441 agatagtagt cgaagggaaa ctgagcgtcg gtcgccacga acagcaccaa ctccccaggc
     4501 tccgcccgag atgccacgta cgatgtcgcc tcctgccact cctccttgat tggaagcgtg
     4561 tacattcgat
//
LOCUS       DATALS010000185         4984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_414661, whole genome shotgun sequence.
ACCESSION   DATALS010000185 DATALS010000000
VERSION     DATALS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414661"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VHS06_04110"
     CDS             <1..1083
                     /locus_tag="VHS06_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987337.1"
                     /translation="DGGVSASRYSYIGGFDSTSNVKAAQLFDGLRAVGTHAHSWVQSF
                     MGLKDLKSRNIVGPDGREHDLVKLVLHYREELGGNRTNEGELASFVAYAQAYPQGFLA
                     LVDTYDTLRSGVPNFLSVALALREVGYRPMGIRIDSGDLSFLSKKARQLFREAGQRYG
                     ADLERLIIVASNDIDEETLLALGQQGHEIDSFGIGTRLVTAGGETLGGVYKLTVAAGV
                     PRIKVSGDSAKITLPGKKRAFRLYAGEAPTPVADLLTLADEAAPQPGERVLCRHPFDE
                     TKRLIVIPSRVEDLYEVIWDGDPNTQTVRPLNETRQYVLDQLAGFRRDHLRPVNPTPY
                     KVSVSAKLYDFLHSLMASEVPIAEIS"
     gene            complement(1175..2122)
                     /locus_tag="VHS06_04115"
     CDS             complement(1175..2122)
                     /locus_tag="VHS06_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX2987338.1"
                     /translation="MRLEELMKMERVVDEVNEWRHCPQNVYTRDGRSLVSKVSTTGRD
                     LKAAIRQSVEMIGGVRKALNPGDRVLLKPNLNSPDPFPAASDLPFLAAVIELLREEGI
                     TDISVGERSGWPWMPTAKVMGKLGVPQFAEEMGVKLIDFDAGPWMDVVLGEKANWWKR
                     VAFPKALKEFDKIVYLPCLKTHFLAGFTMSLKLIVGLTHPTEMLYLHADSRFGKIDEP
                     MFLKMLEMNLPIAPDLIIIDGRKAFVTEGPSKGEMVEPQLVLASADRIAVDAESVKVL
                     QGYPRENLLKGNVWEMPLIRRAIKLGLGARSESEYTVLM"
     gene            complement(2299..3222)
                     /locus_tag="VHS06_04120"
     CDS             complement(2299..3222)
                     /locus_tag="VHS06_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987339.1"
                     /translation="MRRWVPLGLIPLLAVLLSSWVVVCPVSASDGVLVEGDPPLTDGM
                     VARARGFYEWLLDARMNEEQRGDFQQSLVDSWESGDAGQIANILDVLNNADGVGGYSE
                     SEKALLRETIQPNVLDQLRSQAGEGDMGSAWLLALYDRSHQPIADGNPPLTRQVSDAT
                     AEALTFMIAKAFDQEYKVPGADVKDSFGVALVEGYSGYTPEEQEAMAGMPMYWAALGV
                     YWPDAPEEKRAQLRQQWREQLSPLVQEDDASSGGQAASGQQPSSDSDPKPMGWVTFNT
                     LQLAQHQTFLSSVNLIRGNYTAAYTYGLTPR"
     gene            complement(3456..4628)
                     /locus_tag="VHS06_04125"
     CDS             complement(3456..4628)
                     /locus_tag="VHS06_04125"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2987340.1"
                     /translation="MRRVSILGVVVLVCTLLASGSLMLPNVSPAGYTATSPDALEPTV
                     TVSPTANGQFGPRVEVVASGLEIPWAMAFAPDGRLFFTERPGRVRVIVNGQLLPDAVA
                     TLPAAAATGNENGLMGLAIDSDFADNGYIYVAYSYKNAKSQLKNRVSRLTVQGNQAGD
                     EKVLLDEIPGEQIHDGGRLKIGPDGLLYISMGDGSEPGRAQDMNTVSGKVLRINTDGS
                     IPDDNPFPGSPIFTLGHRNPQGLAFQPGTGRLFETEHGTAANDEVNVLQAGRNYGWPL
                     VQGVAKDSRFVDPIIYFTPTVAPSGATFYDGDQLAPWRGNLFFTTLVGQHLHRVVLGG
                     ADGQQVISDERLFEKQYGRLRDVIEGPDGFLYFCTNNRDGRGTPRPDDDRILRIVP"
BASE COUNT          952 a         1636 c         1478 g          918 t
ORIGIN      
        1 gacgggggtg tatcggcatc tcgctactcc tacatcggcg gtttcgactc caccagcaac
       61 gtaaaggctg ctcaactgtt cgacggactc cgcgccgtcg gaacccacgc ccactcctgg
      121 gtgcaaagct tcatggggct gaaggacctc aagagccgca acatcgtcgg gccggacggc
      181 cgcgaacacg atctggtgaa gctggtgctg cattatcgag aggagctagg aggcaatcgc
      241 accaacgagg gcgaacttgc ctccttcgtt gcctacgccc aggcataccc tcagggtttc
      301 ctcgcactgg tggacaccta cgacacgctg aggtccggtg tgccgaactt cctgagcgtt
      361 gccctggcgc tccgcgaggt gggctaccgg ccgatgggga taaggatcga ctccggtgac
      421 ctctccttcc tctccaagaa ggcgaggcag ctgttccgtg aagccgggca gcgctacggc
      481 gccgacttgg aaaggctgat catcgtggcc agcaacgaca tcgacgagga aacgctgctg
      541 gcgctgggcc agcagggaca cgagatcgac tccttcggga tcggaacgcg gctggtcacc
      601 gcggggggag aaaccctcgg gggcgtctac aagcttacag tggccgcggg agttccccgg
      661 atcaaggtat ccggcgactc cgccaagatc accctgccgg gcaagaagag ggcgttcagg
      721 ctgtacgcgg gcgaggcccc cacgcctgtg gccgatcttt tgaccctggc cgacgaggca
      781 gcgccgcagc cgggtgagcg ggttctgtgc cgccacccct tcgacgagac gaagaggttg
      841 atcgtcattc ccagtcgagt ggaggatctg tacgaggtaa tctgggatgg cgatccaaac
      901 acgcagacgg tacgaccgct aaacgaaacc cgacagtacg tcctggacca gctggccggc
      961 ttccggcggg atcacctgcg ccccgtcaac cccaccccct acaaggtgag cgttagcgcc
     1021 aagctgtacg actttctgca ctccctgatg gcctcggaag tgccgattgc ggagatcagc
     1081 taggagttgc gcatgccccg tcggggcacc atcgcctaca cgaatagtag ggcggcgtga
     1141 cgcgagcggg cacagcgtca cgccgcccta ctactcacat caggaccgtg tactcggact
     1201 cgctccgggc gccgaggccc aacttgatcg cccggcggat gaggggcatc tcccacacat
     1261 tccccttcag caggttctcc ctcgggtagc cctgcagcac cttcacgctc tccgcgtcta
     1321 cggcgattcg gtcggcggag gcgagaacta gctgaggttc caccatctcg cccttggagg
     1381 ggccttctgt cacgaatgcc ttccgcccat cgatgatgat caggtcgggg gcgatgggca
     1441 agttcatctc cagcatctta agaaacatcg gctcgtcgat cttgccaaac ctggagtcgg
     1501 catggaggta gagcatctcc gtggggtggg tcaatccgac gatcagcttg aggctcatgg
     1561 tgaagccggc caggaagtga gtcttcaagc agggcaggta gacaatcttg tcgaactcct
     1621 tcagcgcctt gggaaaggcc acccgcttcc accagttggc cttctcgccc aggacgacat
     1681 ccatccaggg gccggcatcg aagtcgatca gcttgacgcc catctcctcg gcaaactgag
     1741 gcacacccaa cttgcccatc accttcgccg tgggcatcca gggccagccc gagcgctcgc
     1801 ccacggagat gtcggtaatc ccctcctccc gcagcagttc gatcaccgcc gccaggaacg
     1861 gcagatcgga cgcggcaggg aagggatcgg ggctgttgag attgggtttc agcagcactc
     1921 ggtctccggg attcagcgct ttccgcacac cgcctatcat ctccacggac tgtctgatag
     1981 ccgccttcag gtcacgccct gtagtggaca ccttcgatac caaggagcga ccatccctgg
     2041 tgtagacgtt ctgagggcag tggcgccact cgttcacttc gtctacgacg cgctccatct
     2101 tcatcaactc ttcgagtcgc attaccgttc ctcctcccgg aattgcgcag ctattctgag
     2161 gcagatcggc cctccgcgca agctgccggc accgttatcc ctgtcgtaag atgaagatcc
     2221 gtagcacaga aggatggagc ccccggcagc gttaccgggg gctccatcct tctccatcga
     2281 acggctcaag gcatcaactc accggggagt cagcccatac gtgtacgccg cagtgtagtt
     2341 gccacgtatc agattcaccg aggagaggaa ggtctgatgc tgagccaact gtagggtgtt
     2401 gaaggtgacc caacccatag gcttcggatc ggagtccgaa gacggttgct gccccgatgc
     2461 cgcctgcccg ccggaagagg catcatcctc ctgcaccagc ggcgacagtt gctccctcca
     2521 ctgctgtcgc agctgagccc gcttctcctc cggggcatca ggccagtaga cgccaagggc
     2581 agcccagtac atcggcatac cggccatagc ctcctgttcc tccggtgtgt agccggagta
     2641 tccctcgacc agagcgaccc caaacgagtc cttcacgtcg gcgcccggca ccttgtactc
     2701 ttggtcgaat gccttggcga tcatgaaggt cagcgcctcc gcagtggcgt ccgatacctg
     2761 tctggtcagc ggcgggttgc catccgcgat cggttgatgg ctccggtcgt aaagcgccag
     2821 cagccaggca gatcccatat ctccttctcc agcctgcgac cggagttggt ccagcacgtt
     2881 gggctggatg gtctccctca gcaacgcctt ctctgactcg gaatacccgc ccaccccatc
     2941 ggcattattg agaacatcca ggatattggc aatctggccg gcatcgccag attcccagga
     3001 atccacaagg ctctgttgaa aatccccgcg ctgctcctcg ttcatcctgg catccaacag
     3061 ccactcgtag aagccccgcg ccctggcaac catgccatcg gtcagcgggg gatctccttc
     3121 caccagcacg ccatcagaag ccgacacagg acacaccacg acccaagacg acaacaggac
     3181 tgccagaagg gggattagcc ccagcggcac ccatcttctc attccatctc cctccacaac
     3241 tctacagtgg ctttgggaga gggtacaagg cgatgagggt tgcgtcagtg tcaaggccgt
     3301 ctcattcttc cgcaagccca tcccggaaag gtgagacgaa ttggttttac ctcaacgatg
     3361 aatcgcctgg tggtagaggc taacctgtct ggccacatct accgtgcgta cacggggccg
     3421 cccccaccat tcattggacg cggcgtgtca gagcactatg gcacgatgcg caggatccgg
     3481 tcgtcgtccg ggcggggggt gccccgaccg tcccggttgt tggtgcagaa gtagaggaag
     3541 ccatccggcc cctcgatcac gtccctgagg cgtccgtact gcttctcgaa cagccgctcg
     3601 tcggagatga cctgctggcc gtcggccccg cccagcacca ctcggtgcag gtgctgccca
     3661 accagcgtgg tgaagaacag gttgccccgc cagggtgcga gctgatcccc atcgtagaag
     3721 gtggctccag agggagcgac ggtcggggtg aagtagatga tcggatcgac gaagcgggaa
     3781 tccttcgcca ccccctgcac cagaggccag ccgtagttcc ttccggcctg gagaacgttg
     3841 acctcgtcgt tggccgccgt cccatgctcc gtctcgaaca gccgccccgt cccaggctgg
     3901 aaggcaagcc cctgagggtt gcggtgcccc agggtgaaga ttggagagcc ggggaaggga
     3961 ttgtcgtccg ggatggagcc atcggtattg atccgcaaca ccttcccgga cacggtgttc
     4021 atatcctgtg cacgcccggg ctccgagcca tcgcccatgc ttatgtacag caaaccatcc
     4081 ggaccgatct tcagccggcc gccgtcgtgg atctgctcgc ccgggatctc gtccagcaac
     4141 accttctcgt cccccgcctg gttcccctgg acggtgagac gcgagacccg gttcttgagt
     4201 tgggacttgg cgttcttgta actgtaggcc acgtagatgt agccgttgtc ggcgaagtcg
     4261 gagtcgatgg ccagccccat caggccattc tcgttgccgg tcgccgcggc cgcaggaagg
     4321 gtggcgacgg catccggaag cagttgcccg ttcacgatca cccgaactcg gcccggccgc
     4381 tccgtgaaga acagccggcc gtccggggcg aaggccatgg cccacggtat ctccaacccg
     4441 gaggccacca cctcaaccct cggtccaaac tggccgtttg ctgtggggct tacagtgacc
     4501 gtgggctcca aggcatccgg gctcgtcgcg gtgtaacctg caggcgacac gttgggtaac
     4561 atcaaggaac cgctggccag cagcgtacaa acgagaacga caacgcccag gatcgacact
     4621 cttcgcatgc agtccccctc gtcccaagtt gccatgcccc ctgagaggcg cgtggtggca
     4681 actcgggcag agagacttcg cagattgcgt acctctacct ctgcacagac tgtaggggcg
     4741 acgcatgcgt cgtcctgatg gctggggaga cccgacccgc cgggcaacgc acgcgtcgcc
     4801 cctacaatag agcgggggga atccgtcgac cccgcccgct catcaagttt ccagtacagt
     4861 cttgctagtt ctcgtacagc tgtatcgcgg aactcacgtc cttcttgccg aggcccgccg
     4921 cgctggcttg ctcgaagtgg gagaccgcca tctgcaccgc ggggaggtct acgccgagct
     4981 cctt
//
LOCUS       DATALS010000186         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_376133, whole genome shotgun sequence.
ACCESSION   DATALS010000186 DATALS010000000
VERSION     DATALS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376133"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VHS06_04130"
     CDS             complement(<1..1304)
                     /locus_tag="VHS06_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987341.1"
                     /translation="MSTMLDRRTDGSIALYIEGDLQFDSRDEAVYHEALALPALALAT
                     TRTRSKLRVLICGGGDGLIARELLKSPEVARIDLVDISPEVLELARTQLAPINGSSLD
                     DQRVRTTVEDAWEYVAKAHEDEEAYNLIISDFTVPQDRKSAGLHSVDWYRRLHHVLAP
                     RGVLAVNGVSPTGATDGYWSIYNSLRVARLHPRPYRIVLHSYREAGYGDDWGFFLASN
                     RPILPAELGDNLPLATPRRALKDVQQLRRLFVFPTEVAARRRSALPTRLDSDLLIRYI
                     YSSQGSSPATTQSGKMWNALAFHRDRAPIPIPDEGTALLPPELVPALAGSGETIDEKT
                     VLERVLRLMPALNRYQTREMISTFLEEPARFLHAIDLPGLVKQLLARASELPRRVVEE
                     LQLLSVKLGSQLVDRDTLLDMGMRVVTIITLVIILGNLLFPDT"
     gene            1539..>1917
                     /locus_tag="VHS06_04135"
     CDS             1539..>1917
                     /locus_tag="VHS06_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX2987342.1"
                     /translation="MSFSWLDLLVGLFVIGGAVRGYGRGLIREGMAFGGLAVGLILAT
                     EWSPEVAGFVKPFVGGGRVLDVLAYLLVLVAVLGAATLLTVLALRLVRAFFAGWVDRF
                     GGALFGACQGAVVAALVLILMIKF"
BASE COUNT          327 a          521 c          667 g          402 t
ORIGIN      
        1 gtgtcgggga agaggagatt ccccaggatg atcaccagtg taatgatggt cactacccgc
       61 atgcccatgt ccagcagggt atccctatcc accagttgcg agccgagttt caccgacagc
      121 agctgcaact cttcgacgac gcggcgaggc agttccgacg ctcgtgccag cagttgcttg
      181 accaggcccg gcaaatcgat ggcatggagg aagcgggccg gctcctccag gaatgtagag
      241 atcatctctc gggtctggta ccggttcagg gcaggcatta gccgcagcac tctctctagt
      301 acggtctttt cgtcgattgt ctcgccggac ccggcgagcg ccggcacgag ttcgggcggc
      361 aggagtgcgg tgccttcgtc ggggatgggg atgggtgcgc ggtctcggtg gaaggcaagg
      421 gcgttccaca tcttgccgct ctgtgtcgta gcgggagaag agccctggct gctgtagatg
      481 tatcgaatga gcaggtcgga gtcgagtctc gtgggaagcg ccgagcgccg ccgagccgcg
      541 acctccgtag ggaagacgaa gagccttcgc aattgctgga cgtccttcag tgcccgtcga
      601 ggtgtagcca gcggcaggtt gtcacccaat tctgcgggga gtatcggccg attggaggcg
      661 agaaagaagc cccagtcgtc gccgtatccg gcttcccgat aggaatggag aacgatgcga
      721 taggggcgag ggtgcagtcg cgccactcgg agggagttgt agatggacca gtagccgtcg
      781 gtggcgcctg tgggagagac cccgttgacg gcgagcaccc cccgtggagc gaggacgtgg
      841 tggagccgcc ggtaccagtc cacggagtgg agcccggccg attttcggtc ctgcggcacg
      901 gtgaaatcgc tgatgatgag attgtaggct tcctcgtcct cgtgtgcctt tgccacgtac
      961 tcccaggcgt cttccaccgt ggtgcgaacc cgttggtcgt ccaggctgct accgttgatg
     1021 ggcgccaatt gggtcctggc gagttccaac acctcggggg atatatcgac caggtcgatt
     1081 cgcgcgacct cgggggattt cagcagctcg cgagcgatca gcccatctcc tccgccacag
     1141 atcaagacgc gcagtttgga tctcgtccgt gtcgttgcga gcgccagggc ggggagcgcc
     1201 agtgcctcgt ggtacactgc ctcatcgcgg ctgtcgaact gaagatcacc ctcgatgtag
     1261 agggctatgg agccgtcggt gcgtctatcc aacatggtgc tcatggcgat gcctccatgg
     1321 cccggccgtc tatcaggccg acacgagttc gggtgttgcc ggccaaggtt ctgcagaaaa
     1381 cgagcagact gcctgagggg cgttaaggtg aggtcgaaag aattctacca tcaggtagat
     1441 gtcgatgtcg aagggtcgtg cgcggtaccg cgctattccc tatgcatggt ggcctcagca
     1501 tgctataact tctcggcaac ttgacggtgt gggggaccat gagctttagc tggttggatc
     1561 tgttggttgg cctattcgtg ataggcgggg cagtcagggg ctacggcaga ggactgattc
     1621 gcgaggggat ggccttcggc ggattggccg tgggcctgat tctggcgacg gaatggagcc
     1681 cggaagttgc cggtttcgtc aagccgttcg tcggcggcgg acgagtactg gacgttctgg
     1741 cctacctgct ggtgttggtg gctgtcctgg gtgccgcgac gctgctcacg gtgctcgccc
     1801 tgcgactggt gagggccttc ttcgcagggt gggttgaccg ttttggaggg gcgctgtttg
     1861 gcgcctgcca gggggcggta gtggctgccc tggtgctaat cttgatgatc aaattcc
//
LOCUS       DATALS010000187         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_279093, whole genome shotgun sequence.
ACCESSION   DATALS010000187 DATALS010000000
VERSION     DATALS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279093"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VHS06_04140"
     CDS             <1..664
                     /locus_tag="VHS06_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX2987343.1"
                     /translation="LRSLANPLNVQGMARYGINPQNTLGVSMVQLRAMARRVGRNHQL
                     ALELWASGIHEARILAALVDDPRLVTEEQAESWVGALDSWDVCDQLCGNLLDRTALAY
                     RKAEEWCGREETFVKRAGFVLIAQLAVHDKKAGDEPFLGFLPLVRREAGDDRNFVKKA
                     VNWALRHIGKRNARLNLAAIEMAREIRQMDSRSARWIASDALRELTGEAVQQKVRTGV
                     NN"
     gene            661..1599
                     /locus_tag="VHS06_04145"
     CDS             661..1599
                     /locus_tag="VHS06_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2987344.1"
                     /translation="MSETHVVFGATGALGSAIVRRLASEGKTVRAVVRDLELAKELLP
                     ASAGIVVHDVRDPAMARAACEGATVAYDCVNVRYSSWEKIMPVVTENLIAAARDAKAR
                     LVFPGNVYGYGPLQRVPAREDHPMAARSKKGRLRIRMEERLMEAHRAGEVPVVIPRFP
                     DFYGPNVTNPLVAPIFRQALKGEAAAWPAKLDVPHDLICIDDAARGCVLLAESEESFG
                     QSWHLPGAGPLTGGELIQLAFEAAGKRPKMQAMSPLMFRIFGIFAPDAGEMVELMYEF
                     EKPLLLDGGKFAAAFPDFSYTPHKVAVAETVKWFEG"
     gene            1596..1970
                     /locus_tag="VHS06_04150"
     CDS             1596..1970
                     /locus_tag="VHS06_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX2987345.1"
                     /translation="MRITFLPGSFSLCRLAPDQEIPNWAIADKSFCSVTYTPQELSIV
                     CPSDVVPADVHCDSGWVILGVEGPMDLSLIGVLASLSVPLADAGIPVFAISTYNTDYL
                     LIMEEKVHAARQALEAAGHQVV"
BASE COUNT          354 a          605 c          700 g          349 t
ORIGIN      
        1 gctacgctcc ctggccaacc ccctgaacgt tcagggaatg gctcgctacg gcatcaatcc
       61 ccagaacacg ctgggggtat ccatggtgca gctgagggcg atggcccgga gggttgggag
      121 gaaccaccaa ctcgcccttg agttatgggc ttctgggatc cacgaggcca ggatcctggc
      181 agcactggtg gacgatcccc gtctggtcac cgaggaacag gcggagagtt gggtcggcgc
      241 cctcgactcc tgggacgtct gcgaccagct ttgtggtaat cttttggaca gaaccgccct
      301 ggcctatcga aaagccgaag agtggtgcgg acgggaagag accttcgtca agcgggcagg
      361 cttcgtcctg atagcgcaac tggccgttca cgacaagaag gctggcgacg agccgtttct
      421 cggctttctg ccgctcgtga ggcgggaggc cggcgacgat cgcaacttcg tcaagaaggc
      481 ggtgaactgg gcgttgcgcc acatcggaaa gcgcaacgcg cggctcaacc tcgccgccat
      541 cgagatggct cgggagatcc ggcagatgga ctcgcgcagc gcccgttgga tagcctcgga
      601 cgccctgcgc gagctgaccg gcgaggcggt tcaacagaaa gtgagaaccg gagtcaacaa
      661 ttgagcgaaa ctcacgtagt ttttggcgcc accggcgccc tgggctcggc gatagtccgg
      721 cgactggcct ccgaagggaa gaccgttcgg gcagtggtgc gggacctgga gctggcgaag
      781 gagctgttgc ccgcgtcggc cggcatcgtg gtgcacgacg tacgggaccc ggcgatggcc
      841 cgtgcggcgt gcgagggggc aactgtagcc tacgactgcg tcaacgtgcg ctacagcagc
      901 tgggagaaga ttatgccggt ggtgacggag aatctgatcg ccgcggcacg tgacgcgaag
      961 gcgaggctgg tcttccccgg caacgtgtac ggttacggtc ctctccagag ggtgcctgcc
     1021 agggaggatc accctatggc agcccgcagc aagaaggggc ggctgcggat tcggatggag
     1081 gagcggctga tggaggcgca tcgggcaggg gaggtgccgg tggtgatccc tcgcttcccg
     1141 gacttctacg gcccgaacgt cacgaacccc ctggtggccc ccatcttccg ccaggcactc
     1201 aagggtgagg cagcggcgtg gcccgcgaag ttggacgtgc cccacgacct gatctgcatc
     1261 gacgacgcgg cacggggctg cgtgctcctc gccgagtcgg aggagagctt cggccagagc
     1321 tggcatctgc ccggggccgg tcccctgacc ggtggtgagc tgatccagct ggccttcgag
     1381 gccgcgggaa aacggcccaa gatgcaggcc atgtcgcctc tgatgttccg aatcttcggt
     1441 atcttcgccc cggacgcggg ggagatggtg gagctgatgt acgagttcga gaagccgctg
     1501 ctgctggacg ggggcaagtt cgccgccgcc ttcccggact tcagctacac gccgcataag
     1561 gtggccgtgg ccgagacggt gaagtggttc gagggatgag gatcaccttc ctcccgggca
     1621 gcttctcctt atgtcgcctt gcgcccgatc aggagatacc caactgggcg attgccgaca
     1681 agagcttctg ctcggtgacc tacactccgc aggagctctc catcgtctgc ccgagcgacg
     1741 tggtgccagc cgacgttcac tgcgactcgg gctgggtgat cctcggggta gaggggccga
     1801 tggacctctc cctgatcggg gtcctggcgt cgctgtcggt ccccctggcg gacgcgggga
     1861 tcccggtctt cgccatctcc acctacaaca ccgactacct gttgatcatg gaggagaagg
     1921 tgcatgcagc ccggcaggct ctggaggccg cggggcacca ggtcgtgtag ccgcacccat
     1981 tgtcggcgcg tactgaagat tgacgttg
//
LOCUS       DATALS010000188         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_199749, whole genome shotgun sequence.
ACCESSION   DATALS010000188 DATALS010000000
VERSION     DATALS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199749"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            474..>1503
                     /locus_tag="VHS06_04155"
     CDS             474..>1503
                     /locus_tag="VHS06_04155"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX2987346.1"
                     /translation="MANGRQGSMDRRRFLKMVGAAAGAVTLADLAAACSSSSPAPAAT
                     SAPAAAPAATKPAASAPAAPAASTKGKTIRVAYVHGAGGTSEMAANDFVKFISEQTKG
                     EYTAQIFPGGQLGGERDMVESQQMGSIEVGWYGGYLVSNLAPEYGNILETPYVMQSVA
                     HFRKVVDGSEIKPAYDKMLKDKGIRHIAWTNRGPRYLTSNKAVKTPADVKGMKIRVPE
                     IETYVAAWKALGATVVPMAFPEVFMALKQGTIDAQENPLENAMNGSFYDVQKYVNLTA
                     HIYTGFELTVSEKWYSALPADVQKIVTDGIVLLCKNQDKYQAEAEDKLAKDLQAKGMT
                     FNQPDLASF"
BASE COUNT          328 a          490 c          430 g          255 t
ORIGIN      
        1 ccaccatccg gcgcaataat acactcatat cgacgatctg tatagatgag tctgtgtttc
       61 tgttgacatc aggtggtctg atgatataat tccacttagt tgaagttcag cttagttcca
      121 gtccggcgct ctctggttgc cccgccacca cgctcgtctg gagagcaggt ctgccgggtc
      181 cccgtgagcg accgaccggg ggatcaggta gttcattttc gcacctcggg agctgtctct
      241 ccagtcatca gatcataaga tgaggagatt gtgacctacc atctgtcatt gctcagccca
      301 gctcgagatg agagcagcgt cagcaagggc tgaacacgac tcagccaata ccggcgacct
      361 gttggcccac atatccaatt ccaatgcgcc caaaatgggt gcggcaccca cggcatcctg
      421 catatgcttg cgtgaactaa gcgctagtag atatggatga aaggggagtt gagatggcca
      481 acggcagaca gggtagcatg gatcggcggc gcttcctcaa gatggtcggc gcagcggccg
      541 gggcagtgac cctggccgac ctcgcggccg cctgcagttc atcctcaccg gcgcccgcag
      601 caacctccgc ccccgccgcg gccccagcgg caaccaagcc cgcagcctct gccccagcgg
      661 ctccggcggc aagcaccaag ggcaagacca tccgggtcgc ctacgtgcac ggcgcgggtg
      721 gaacctcaga gatggccgcg aacgacttcg tcaagttcat ctccgagcag actaagggtg
      781 agtacactgc ccagatcttc ccgggcggcc agttgggcgg cgagcgcgac atggtggagt
      841 cgcagcagat gggctccatc gaggtcggct ggtacggcgg ctatctggtc tccaacctgg
      901 ctcccgagta cggcaacatc ctcgagaccc cctacgtcat gcagagcgtg gcgcacttcc
      961 gcaaggtagt cgacggctcc gagatcaagc cggcatacga caagatgctg aaagacaagg
     1021 gaatccgcca catcgcctgg accaaccgcg gcccccgcta cctcaccagc aacaaggcag
     1081 tcaagactcc ggccgacgtc aaggggatga agatccgcgt ccccgagatc gagacctacg
     1141 tggcggcatg gaaagccctc ggcgccaccg tcgtccccat ggccttcccc gaggtgttca
     1201 tggcgctaaa gcagggcacc atcgacgccc aggagaaccc cctcgagaac gccatgaacg
     1261 gctccttcta cgacgtgcag aagtacgtca acctcaccgc tcacatctac accggcttcg
     1321 agctcacggt cagcgagaag tggtactccg cgcttccggc cgacgtccag aagatcgtca
     1381 ccgacggcat cgtcctgctg tgcaagaacc aggacaagta ccaggccgag gccgaggaca
     1441 agctggccaa ggacctccag gccaagggca tgaccttcaa ccagcccgat ctggcatcat
     1501 tcc
//
LOCUS       DATALS010000189         3474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_243708, whole genome shotgun sequence.
ACCESSION   DATALS010000189 DATALS010000000
VERSION     DATALS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243708"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VHS06_04160"
     CDS             complement(<1..468)
                     /locus_tag="VHS06_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX2987347.1"
                     /translation="MYVIGTAGHVDHGKSTLVQALTGIDPDRLREEKERGMTIDLGFA
                     WLKLPSGQEVSIVDVPGHERFIKNMLAGVGGIDVALLVVAADEGPMPQTDEHLAILDL
                     LGISRGVVAITKSDLVDADWLELVKAEVEERLAPTSLANASLIPVSSVTREGLP"
     gene            579..1295
                     /locus_tag="VHS06_04165"
     CDS             579..1295
                     /locus_tag="VHS06_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2987348.1"
                     /translation="MARLRYLGISFVEIITDDGKVVYIDPCISINPDCPITLNDIEHA
                     DLVLVTHMAKDHSSDFIPLVKRTGAQLVCARDTARAAVSAGISEDRIRVVVSGVAVER
                     AGVRVKVLKTEHGSWWTEGDHPVFDLSLGYIVYAGDGKGVYHVGDTSIFGDFALFGRL
                     YRPKVMLTPIGMFPGAITEMDPWEAAIATGMVAPEIAIPIHYDRKTQEEYPRHFANHL
                     ATESPRTVVKALAPGETIEI"
     gene            1353..2009
                     /locus_tag="VHS06_04170"
     CDS             1353..2009
                     /locus_tag="VHS06_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2987349.1"
                     /translation="MSEEQSRRSGRFAWGVVIAFFLLLAVVNTTLTLSGAVDSWNQAT
                     TQQIYQLIRPWLNPPMIAITLLGYWEPLLAITLAVAIWAIVRGRKLDAWLLFGVYVVS
                     LGLQELTKQLVRYPRPHFIVPPSPLMPLESYAYVSSHALQAISVLGFATVIACGLIGR
                     RWVRVAVVAVSILLIAGIGFSRVYLGLHWVNDVLGGYLYGVVLLLVASRLHLSERRRS
                     "
     gene            complement(1987..3375)
                     /gene="selA"
                     /locus_tag="VHS06_04175"
     CDS             complement(1987..3375)
                     /gene="selA"
                     /locus_tag="VHS06_04175"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX2987350.1"
                     /translation="MSDTMASLRQLPSVNRLLREQQVEELSNLYSHELLVQAIRDTLQ
                     EARDAIRDGGNLPAITGLIEAVREHLEATFRPSLRPVINATGVIIHTNLGRAPLSDDA
                     LRAVRDVAAGYSNLEYRLEEGTRGSRHEHISDLVRRISGAEDALVVNNNASAVLLILS
                     ALAQGREVIVSRGQAVEIGGGFRIPDVLRQSGARLVEVGTTNRTYIRDYEEAITPETG
                     ILLYVHPSNFRVEGFVHSATIEELADLGRRLDIPVVDDLGSGSLLNSAEFGLQHEPMV
                     QERVAAGADLVAFSGDKLLGGPQAGLIAGKAELVEKLRHHPLARALRVDKMTIAALAA
                     TLNHYLRGEALTRVPVWRMISTPIEDLESRGGRLARRLRQAGLAAETMPGLSTIGGGS
                     LPGETLPTRLVVVSPTPDQPSAMEMAAALRGQDPPLVARLERDRLLLDLRTVPPQEDR
                     VVADHLKNVAAR"
BASE COUNT          594 a         1075 c         1103 g          702 t
ORIGIN      
        1 gggcagcccc tcacgggtaa ccgatgagac ggggatcagg gaggcgttgg cgagggatgt
       61 aggagcgagt cgttcctcca cctcggcttt caccagttca agccaatcgg catccaccag
      121 atccgactta gtgatggcga cgacgccgcg agagatgccc agcaggtcga ggattgccag
      181 atgctcgtcg gtctgtggca tggggccctc gtcggcggcg accaccagca gcgctacgtc
      241 tatgccgccc acgccggcca gcatattctt gatgaagcgc tcgtgtccgg gtacgtcgac
      301 tatgctcact tcctgcccgc tgggtagctt gagccaggcg aagcccaggt cgatcgtcat
      361 tcccctctcc ttctcctctc ggagtcggtc ggggtcgatg ccggtcagcg cctgtaccag
      421 tgtggatttt ccgtggtcca cgtgccccgc ggtcccgatg acgtacactc ttcttcctcg
      481 tcggtgaaat gcctataagg tattatagct tctgcgaggt gggaagcgcc tgaagcggcg
      541 gcttccatgc cgattgttgt gcgcaaggag gcgaggaaat ggccagactt cgttacctgg
      601 ggatctcctt cgtcgagatc atcaccgacg atggaaaggt cgtctacatc gatccgtgca
      661 tctccatcaa tccggattgc cctatcaccc tgaacgacat cgagcacgcc gacctggtgc
      721 tggtcaccca catggcgaag gatcactcca gcgacttcat cccgctggtg aagaggacgg
      781 gggcgcagct cgtgtgcgct cgggatacgg ctcgagcggc cgtaagcgcg ggcatatcag
      841 aagatcggat cagggtggtt gtgtccggcg tggcggtgga gagggcaggg gttcgtgtca
      901 aggtactgaa gacggagcat gggtcgtggt ggaccgaggg ggatcacccg gtcttcgatc
      961 tatcgctcgg ctacatcgtg tacgcgggcg acgggaaggg tgtctaccac gttggggata
     1021 cttccatctt cggcgacttc gcgctcttcg gacgccttta ccgcccgaag gtgatgctta
     1081 cgcccattgg catgttcccc ggcgcgatca ctgagatgga cccgtgggag gccgccatcg
     1141 cgacgggaat ggttgcaccg gaaatcgcca tccccattca ctacgaccgc aagacccagg
     1201 aggagtaccc gaggcacttc gccaaccacc tcgctaccga gtcgccccgt acggttgtga
     1261 aggcgctggc tccgggcgag acgatcgaga tataggcagg agagaacggg cccctatcga
     1321 cgccggtagg gggcaattgg tgacgattcg agttgagcga agagcagtct cgtcgatctg
     1381 gccgctttgc gtggggagtg gtcatcgctt tctttctgct gctggcggtg gtgaacacga
     1441 ccctcaccct atcaggcgcc gtagattcct ggaatcaggc aaccacgcag cagatctatc
     1501 aactgattcg gccatggctc aatcccccga tgatcgccat cactctactg ggttattggg
     1561 aaccgctgct ggcgatcacg ctggctgttg ccatctgggc aatcgtgagg gggagaaaac
     1621 tggacgcgtg gctgctcttc ggagtgtacg tcgtctctct aggccttcag gaattgacca
     1681 agcagttggt gcgttacccg cggccgcact tcatcgttcc gccttcaccg ctgatgccgt
     1741 tggagagcta cgcctacgtc agcagccatg ccttgcaggc catctccgtt ctcggctttg
     1801 ccacggtaat cgcctgtggt ctgatcggga gacggtgggt tcgggtggcg gtggtggctg
     1861 tatcgatcct cctgatcgcg ggcatcgggt ttagccgtgt ctatctcggg ttgcactggg
     1921 tgaacgacgt gctgggaggc tatttgtacg gcgtggtcct cttgctggtg gcgtcccggt
     1981 tgcacctcag cgagcggcga cgttcttgag atgatcggct accactcggt cctcctgcgg
     2041 cgggacggtg cgcagatcca gcaacaggcg gtcgcgctcc agtctggcca ccaacggcgg
     2101 atcctgtccg cggagggccg cggccatctc catggctgaa ggctgatccg gagtcgggct
     2161 cacgacgacc agccgggtgg ggagcgtctc accagggagt gagcctccgc cgatggtcga
     2221 gaggcccggc atggtctcgg cagccaggcc tgcctggcgg agccgccggg ccaaccttcc
     2281 gccgcgcgat tcgagatcct cgattggggt ggagatcatc cgccataccg gcaccctggt
     2341 cagcgcctct ccgcgcaggt agtgattgag tgtggctgcc agggcggcta tggtcatctt
     2401 gtccacgcgg agcgcccggg cgagcggatg gtggcgcaac ttctccacca gttcggcttt
     2461 ccctgcgatt agccctgctt gcggacctcc cagaagcttg tcgccgctga aggctaccag
     2521 gtccgcgccg gctgccactc gctcctgcac catcggctcg tgctgcagcc caaactcagc
     2581 gctgttcagc aacgatccgc tgcccaggtc gtccaccacg gggatgtcca gacggcggcc
     2641 gagatcggcc aattcctcga tggtcgccga gtggacgaac ccctccaccc ggaagttgct
     2701 ggggtggacg tagagcagga taccggtctc cggggtgatg gcctcctcgt agtcgcggat
     2761 gtaggtgcgg ttggtggtcc ccacctccac cagcctggcc ccgctctggc gaagcacgtc
     2821 cggtatcctg aatccgccgc cgatctcaac cgcctgtccc ctggagacga tcacctcccg
     2881 accctgtgcc agcgccgaga ggatcagcaa caccgcgctc gcgttgttat tcaccaccag
     2941 ggcgtcctcg gcgccgctta tccgccgcac caggtcggag atgtgttcgt gacgggagcc
     3001 tcgcgtgccc tcctccagcc tgtactccag gttgctatat ccggcggcga cgtcacgcac
     3061 cgcccgcagt gcatcgtcgc tcaatggggc ccggccgagg ttggtgtgga ttattacgcc
     3121 ggtagcgttg atcacggggc gcagcgaggg gcggaatgtg gcctccaggt gttcacgcac
     3181 agcttcgatc agaccggtga tcgctggcag gttgccgccg tcgcggatag cgtcgcgggc
     3241 ttcctgcagc gtatcccgga tcgcctgtac cagcagttca tgggagtata ggttcgagag
     3301 ttcctcaacc tgctgctccc tcagcagccg gttaacactg ggaagctgcc tcaaggatgc
     3361 catcgtgtca ctcatctttg ccgttctcac caccatccat caccaattcg gcatacgaca
     3421 agtatagtac agatacaacc cagccgaagc cagaccgcct acggggagac gggg
//
LOCUS       DATALS010000190         4127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_376654, whole genome shotgun sequence.
ACCESSION   DATALS010000190 DATALS010000000
VERSION     DATALS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376654"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..478)
                     /locus_tag="VHS06_04180"
     CDS             complement(107..478)
                     /locus_tag="VHS06_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2987351.1"
                     /translation="MAENIDRFEFPDFIKDLPEVDVPYAGLRGLSAQSDHGQVVFNEA
                     DQEVDVVEHSHGEQWGVVLEGRVDFVIRGQKYSYYAGDTYYIPAGISHGVKFFPGARS
                     LDFFSDRERYPIKHQKVHGRA"
     gene            complement(864..1172)
                     /locus_tag="VHS06_04185"
     CDS             complement(864..1172)
                     /locus_tag="VHS06_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987352.1"
                     /translation="MIRTTQLPVRKGSALSLSGLRRRERSITVRIFDGGHEVDSFQLS
                     TAQLLRSEMDAIYAHLPKRDATVLFHGVEVESLPLGIMKARREVELDNISAALSFLNN
                     "
     gene            1162..1884
                     /locus_tag="VHS06_04190"
     CDS             1162..1884
                     /locus_tag="VHS06_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX2987353.1"
                     /translation="MRIIVTTSMSPSTRNAITVGIAGLGDFQHFLEAPRLDTCYDMRG
                     FSSTQDRKYVTSTVPLKSLKYFGVMALDSEKLLDDVGWRILQELQQDARLSYAELGRR
                     VGLTLPAVAERVRRLEEAGVIVGYRARVNRAKLGMPITAFIRVATPSEKNGNVLVAKA
                     KELPEVLECHRVTGDDSYVLKVAVTSVLHLEKFISKITVYGQPTTSIILSSPVSHKVV
                     GPLDPEQIPEDHSHHEESASIG"
     gene            complement(1895..2635)
                     /locus_tag="VHS06_04195"
     CDS             complement(1895..2635)
                     /locus_tag="VHS06_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987354.1"
                     /translation="MSMIRPEEGPRTLDIVVSSPLSVTESLALDEENTRRAADSRQWT
                     LRLWWGGKPTVVMGRSEKSEVVANLDYCRRLGVEVLQRSSGGGTVLQTSDVLNYSITG
                     PAPHLLDVRGTFSIGAKLLIGALANWGIQAHPRGISDVAVGELKISGNAMAKRWGGLL
                     LHGTLLRDLDLDMVENCLLHPPREPDYRGRRSHRDFITSLRALGIEVSRTEMEETVVR
                     TACQLVEEGAFSLLPEAPTKAERNSGEP"
     gene            complement(2626..3870)
                     /locus_tag="VHS06_04200"
     CDS             complement(2626..3870)
                     /locus_tag="VHS06_04200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2987355.1"
                     /translation="MVSTARIGENGDIQLPDVYRRRWGLDPGADLMVQETPEGLLIRR
                     ADPPLTKLYVEPTNLCNLRCRTCVRNSWSDPQGTMSIELYRRMADGLRNLPSLRQVNF
                     WGIGEPLLHPDIVEMVELAKGLGARTQMITNGLLLTRELGAGLLSVGLDSLVVSVDGA
                     SPEVQADVRSGADLNRMKNNLIGLREVCLEREMAWPEIGIEFVAMRSNVDQLREIRSL
                     AVFLGASFIVVTNLLPYNREMSDEILYGMYAGGCYPMYRTRWTPEIILPRMDNRQEVM
                     EPVMRMLEHTNSNDFPRARLDGAGGYCRFVGEGAAAVGWSGDVSPCIALMHSYPVYVM
                     GRQKQIRRYVLGNVGEEEITSIWDKEEYVAFRRRVQRWEFSPCTDCGGCHLADFNEED
                     CFGNTHPVCGDCLWAKGVIQCP"
BASE COUNT          858 a         1320 c         1178 g          771 t
ORIGIN      
        1 gcggacacgc aggtccgccc ctaccaccta tcgggcactg gatggcgatg gaaaggcgtg
       61 agactggcga cagagaaagg cacccgccct acccccacac cccgcgtcag gcccgcccgt
      121 gcaccttctg gtgcttgatc gggtaacgct ctcgatccga gaagaagtcc agggatcggg
      181 cgcctggaaa gaacttgaca ccatgggaaa tacccgcggg aatgtaatag gtatccccag
      241 catagtacga gtacttctga ccgcggatga cgaagtccac ccttccttcc agcacaacgc
      301 cccactgctc gccatgagag tgctcgacca cgtccacctc ctggtctgcc tcgttgaaaa
      361 ccacctggcc gtgatcgctc tgtgcagaca atccgcgcag ccccgcatag ggcacatcta
      421 cttcaggaag atcctttatg aagtcaggga actcgaagcg atcgatgttt tccgccatca
      481 gggcaactcc tcaaacaatg agacgtccga aacaggggcg gcagcctccc aacacgatcc
      541 atgcacctcg ggcttataag accacagtgc aggagagcca acaggcgcga tcttggagta
      601 ggggcggcaa taccgcaacc aaacggcttt caggcaagtt gtgggcctgg caatccgcgg
      661 tggtgccgcg cagtagagcc gatgagtagg ggcaaagcat gcgtcgccct tgcaggtaga
      721 aaccattgtg gtcggtcaac agtggctcgg caagatgatc ccaaatagag ggggaaaggg
      781 ccgcagccca gacagaaggc ggcggcccta ggggggtatt gtataggttt cggcgtagcc
      841 gccgaagcct ttgttgcgcc aggtcagttg ttcaggaatg agagcgccgc gctgatatta
      901 tccagctcaa cctcgcggcg agccttcatg atgcccagcg gaagcgactc cacttcgacg
      961 ccgtgaaaca ggaccgttgc atcccgcttc ggcagatggg cgtaaatggc gtccatctcc
     1021 gagcgaagaa gctgggccgt ggaaagctgg aagctatcca cttcgtgccc gccgtcgaaa
     1081 atgcgaacgg tgatggacct ctcgcggcga cgcaaacccg acagtgaaag ggctgatccc
     1141 ttccgaacag gaagctgagt cgtgcggatc atcgtcacca cctccatgtc gccatcgaca
     1201 aggaacgcga taaccgtcgg aatagccggt ctcggcgatt ttcaacattt cctggaagca
     1261 cctcgcctcg acacgtgcta tgatatgcgc gggttctcct caacccaaga cagaaagtac
     1321 gttacttcca cggtcccact taagagccta aagtatttcg gggtaatggc tttggattct
     1381 gaaaagctcc tcgacgacgt tggctggcgc atcctgcagg agctccagca ggatgcacgg
     1441 ctgagctacg ccgagcttgg gcggcgggtc gggctcactc tacccgcagt agcagagaga
     1501 gttcgaagac tcgaagaggc gggggtgatt gttggctaca gggcaagggt gaaccgggca
     1561 aagctcggca tgcccattac cgccttcatt cgcgtggcca cccccagcga gaagaatggg
     1621 aatgtcctgg tggccaaggc aaaggagttg cccgaggtgt tggagtgcca cagggttacg
     1681 ggagacgaca gctacgtcct caaagtagcg gtcacgtcgg tgctccacct tgagaagttc
     1741 atctcaaaaa tcaccgtgta cggccagccg acgacgtcaa tcattctctc gtcaccggtg
     1801 tcccacaagg tggtcgggcc gctggatccg gagcaaattc cggaggacca ttcccatcac
     1861 gaggaatcgg cctcgatcgg atagaccaag gaactcaggg ttcgcctgag ttcctctcag
     1921 ctttcgtcgg ggcctctggg agcagactaa atgccccctc ctcgaccaat tggcaggcag
     1981 tccgcaccac cgtctcctcc atctcggtcc tagacacctc tatgcccagt gccctcaggc
     2041 tggtgatgaa gtcccggtgg ctcctacggc ctcggtaatc tggttcccgc ggggggtgaa
     2101 gcagacagtt ctcgaccata tccaggtcca ggtcgcgcag cagcgtgccg tggagcaaca
     2161 gaccacccca ccgcttggcc atggcgttgc ccgagatctt caattcgccc actgcgacgt
     2221 cggaaatccc gcgtgggtga gcctggatcc cccagttcgc cagggccccg atgagcagct
     2281 tcgccccgat tgagaaggtg ccccgtacat ccagcaggtg gggagccggc ccggtgatgg
     2341 agtagttgag gacatccgag gtctgtagca ccgttccgcc gcccgaggag cgctgcagca
     2401 cctctacccc caaccggcga cagtaatcca ggttcgccac cacctcgctc ttctccgaac
     2461 gccccatcac cacggtaggc ttcccgcccc accacagccg cagagtccac tgccggctgt
     2521 cggcagcccg tcgggtgttc tcctcgtcca gggccagact ctccgtcacg gagagtggcg
     2581 aagacaccac gatatcaagg gttcgcgggc cctcctcggg tcgaatcatg gacactggat
     2641 cacccccttg gcccagaggc agtcgccaca taccgggtgg gtgttgccga agcagtcttc
     2701 ctcgttgaag tcggcgagat ggcaccctcc acagtcggtg cacggtgaaa actcccagcg
     2761 ctgcacccgt cttcgaaacg ccacgtactc ctccttgtcc cagatagagg tgatctcctc
     2821 ctcgcccacg ttgcccagaa cgtaacggcg gatctgcttc tgcctgccca tcacgtacac
     2881 cgggtagctg tgcatcaagg cgatgcaggg gctcacgtca ccgctccagc ccactgccgc
     2941 tgcaccctca ccgacgaaac ggcagtaccc accggcgccg tccaaccgag ccctgggaaa
     3001 gtcgttggaa ttggtgtgct ccagcatccg catcaccggc tccatcacct cctgccggtt
     3061 gtccatcctg ggaaggatga tctccggggt ccaacgggta cgatacatag gataacagcc
     3121 gccggcgtac atcccgtaga ggatctcgtc gctcatctcg cggttgtagg gaagcaggtt
     3181 ggtgacgacg atgaagctcg cgcccaggaa taccgccaac gaccggatct ccctaagttg
     3241 gtcgacgttg ctgcgcatgg cgacgaactc gatgccgatt tccggccacg ccatctcgcg
     3301 ctccaggcag acttccctga gtccaatcag attgttcttc atgcggttga ggtccgcgcc
     3361 ggagcgaacg tccgcctgga cctccggtga ggcaccgtcc acagagacga cgaggctgtc
     3421 caacccgacg gaaagcaggc cggcgccgag ttcacgggtc agcagcagcc catttgtgat
     3481 catctgggtg cgcgcaccga gccctttcgc cagttccacc atctccacga tgtccgggtg
     3541 gagcagcggc tcgccgatcc cccagaagtt gacctgccgc agagacggca ggttgcgcag
     3601 cccatcagcc atccggcggt agagctctat gctcatggta ccctggggat cgctccagga
     3661 gttgcggacg caggtgcgac atctgagatt acacaggttg gtcggctcta cgtacagctt
     3721 cgtcagtgga gggtcggcac gccggatcag caacccttca ggggtctcct ggaccatgag
     3781 atccgcaccg gggtccagcc cccagcgacg tcgatagaca tcaggcaact ggatatcgcc
     3841 attttcgccg atccgcgctg ttgataccat cgtccgtatc ccctcagcac ttcttttccg
     3901 gacagcacac ggccgaccct cggtttccgg cggcctgtgc atagacgatt atctgtcttc
     3961 ccttggtttc tcccgccttc ggtatcagct cgtactatgc cgtcgcaacc ccagatcgtc
     4021 attataccag cccgagattc ggcaccgtac gcacgctcgg ttaaccccgg ccactcctaa
     4081 gtacatcacc gaaacgagat cgcatactcg gctgcatgat ctatgat
//
LOCUS       DATALS010000191         4695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_377011, whole genome shotgun sequence.
ACCESSION   DATALS010000191 DATALS010000000
VERSION     DATALS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377011"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1619)
                     /locus_tag="VHS06_04205"
     CDS             complement(240..1619)
                     /locus_tag="VHS06_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987356.1"
                     /translation="MTERPDPFSRRISRSQFLKYTVGLGAAMLSASLLPPPVAVAAAL
                     PPLPRPEGLHHALSHNPLPFGHEEFIAGLRRWEEANQEPAGFAADFRQLLLDTARRCV
                     ADQGAWINPGINYPEFFFCRDSFWLLAGLNDIHLSTVTVKRFREDQMLNADGHAATAF
                     YLDGSSPQWRDRDDESTLFYILHNYLLFRLGGEIGIPSMEAAHRFVAAHVREGRYLTT
                     GETRSFSEDGVPDERGAYHYWLDTLRIGTGEGSPEVIAYNQGLLCVALRALKSMGIAL
                     GEGELERAEAAYAGMVNPEDECSIPQREGARLMDVSSLVGEALSLYLFDRPILSRERV
                     EATFQRLNRCARVDYPDGAFLGYKVLCEADGKYLDSGQFLCSTFNSPGRYQNGGSWLL
                     YDGLALYAAARHGVEGAAELFAQRLRSEVRSERSSHEFLSTNPSHSGPNRPEELGYGW
                     NAFLVNLLP"
     gene            1815..2030
                     /locus_tag="VHS06_04210"
     CDS             1815..2030
                     /locus_tag="VHS06_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX2987357.1"
                     /translation="MSKRLYRSRGNRMIAGVCEGIAESYDLDPTIVRLGFVLLALFHG
                     IGLVAYLALWIVMPARSNANGSQGPRA"
     gene            2117..2737
                     /locus_tag="VHS06_04215"
     CDS             2117..2737
                     /locus_tag="VHS06_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX2987358.1"
                     /translation="MTKRLYKSRTDRMIAGVCGGLAEYFDIDPVLVRLLFLLLAFLTG
                     TGFIIYPILWIVMPEQTSLQRPPRDVVWENIGKMQEDVERIGEEIHAGTRGSTVATEG
                     SATEGTTVEERITENGGRQSKAGEPGLEGTVEDRSYYDTEPYNRPHRSRTDRQFIGGV
                     ILLVLGVILLMQNLGIFWWASVWRLWPLVLVAIGVYLLYKRSRPRL"
     gene            complement(2786..3697)
                     /locus_tag="VHS06_04220"
     CDS             complement(2786..3697)
                     /locus_tag="VHS06_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2987359.1"
                     /translation="MKGFRKLLVPLDGSRLAEAVLPATHLLAERFGATVSLLHVMEQG
                     APETIHGERHLDNLKEAEEYLNGVASECDCQASSVEIHVHPNQERDVVASIIGHAREF
                     EADLIILATHGWGGMRDLLVGSIAQQVLRRGVLPVLLIRPTPSGGAPPFEGKRILVPL
                     DGKQAHDGVVLPVVERVAEALGSSLHLLLVVPTLSTMPRNQAPVNTLSPSATAAALDM
                     EEDEGREYLARLAGEMMEKKITVSAEVRRGDAASSVVDVVREIDPDLIVMATHGRAGL
                     GAFWSASVGSKILARIQQPLLMVRVPE"
     gene            complement(3697..>4695)
                     /locus_tag="VHS06_04225"
     CDS             complement(3697..>4695)
                     /locus_tag="VHS06_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX2987360.1"
                     /translation="ERYPRPVVWGMWLLGELVAMATDLAEFLGAALGFNLLLGIPLFV
                     AAVITGVVTFIILGLQRYGFRPLEAVITVMVGIIAACYLLETIFSPPDWGQVGLAVVQ
                     PRFSGTESVLLATGILGATVMPHVIFLHSALTQDRIVVRDPARLRRLFHFEVVDVVIA
                     MGIAGLVNAAMLIMAASTFNAHGISGVGSIEEAHKTLTPLLGSASSVIFAVSLLASGL
                     SSTTVGTMSGQVIMQGFLHRTIPLWFRRLFTMVPALIVAGIGLDPTRTLVVSQVVLSF
                     AIPFALIPLVMFTRNRAIMGPLVNKRLTTVVASVVAALIVVLNLFLLYQTLFGGGA"
BASE COUNT          892 a         1493 c         1554 g          756 t
ORIGIN      
        1 gaatcctggg agcgtgggcg tctcgcccgc atggccatga tgtggctacg cgtggggggg
       61 gtacgattca cacgggacgg acaggatgag ccggatagac aggatgggga caaaatgatc
      121 ggatcctcat cctgtacatc cgtctcaccc tgtccatcct gttcggtttc tctctgcgga
      181 ccttcgcccc tacgcgagaa cggtaggggg cacctgtgcc aacgtcgcgc cgtttccggc
      241 tacggcagca ggttcaccag gaaggcgttc cagccgtagc ccagctcctc ggggcggttg
      301 ggcccgctgt gggaggggtt cgtgctgagg aactcgtggg aggagcgctc cgacctcacc
      361 tcggagcgga gccgctgggc gaacagctcc gccgcgccct ccaccccgtg gcgcgccgcg
      421 gcgtacagcg cgaggccgtc gtacagcagc cagctcccgc cgttctggta gcggccgggg
      481 ctgttgaaag tggaacagag gaactgcccc gaatccaaat acttcccgtc cgcctcacac
      541 agcaccttgt agcccaggaa ggcgccgtcc gggtagtcca ccctggcgca gcggttcagc
      601 cgctggaagg tcgcctccac ccgctcccgg gagagtatgg gccggtcgaa gaggtagagg
      661 gagagggcct ctcccaccag ggaggagacg tccatcaacc gcgcgccctc ccgctgggga
      721 atggagcact catcctccgg attcaccatg cctgcgtaag cggcctcggc gcgttccagc
      781 tcgccctccc ccagcgctat ccccatcgac ttcagcgccc gcagcgccac gcacagcagc
      841 ccctggttgt aggcgatcac ctccggcgac ccctcacccg tgccgatgcg cagcgtgtcc
      901 agccagtagt ggtaggcccc cctctcgtcg ggcaccccat cctcgctgaa ggagcgggtc
      961 tcaccggtgg tcaggtagcg cccctccctc acgtgcgccg cgacgaagcg gtgggcggcc
     1021 tccatcgacg ggatgccgat ctcacctccc agtctgaaca ggaggtagtt gtggaggatg
     1081 tagaaaagcg tgctctcgtc gtctcggtcc ctccactggg ggctggagcc gtccaggtag
     1141 aaggcggtgg ccgcgtggcc gtcggcgttg agcatctggt cctcgcggaa gcgcttcacc
     1201 gtgacggtgg agaggtggat atcgttcagc ccggcgagca gccagaagga atcgcggcag
     1261 aagaagaact ccgggtagtt gatacccggg ttgatccagg caccctggtc ggcaacacag
     1321 cggcgcgcgg tgtcgagaag cagctgcctg aagtcggcgg cgaagccggc cggctcctgg
     1381 ttggcctcct cccaacgccg aaggcctgcg atgaactcct cgtggccgaa gggaagcggg
     1441 ttgtgggaga gcgcgtggtg caacccctcc ggccgcggca gagggggcag ggccgcggcg
     1501 acggcgacgg gcggagggag aaggctggcc gagagcattg ccgcgccgag acccacggtg
     1561 tacttcagga actgagatcg gctgattcgg cgggagaagg gatcagggcg ttcagtcatg
     1621 cggaaccaaa ccaccagctg agcacagaga ttatctggat ggtacgggcg aagtcctcta
     1681 ccatccatcg tatggccgtg tgacaggata tcagtctagc acagcgggca gggggttgac
     1741 gaaaccttcc ggtcggctcc ctgtctaata gatcgacaac gcaagacgag ccgagctacg
     1801 gcgggaggga tagaatgtcc aagcggttgt acaggagcag agggaatcgg atgatcgccg
     1861 gcgtgtgcga ggggatagcc gagagctacg acctggaccc caccatcgta cgcctaggct
     1921 tcgtgctgct ggcgctgttc cacgggatcg ggcttgtggc ctacctcgcc ctctggatcg
     1981 taatgccggc acgttccaac gccaacggca gccagggacc aagggcatag cccggcaggg
     2041 caaaccaatg ccacccgttt ccacctcggt ggtgcgacga gactgaaccg agcgatgcgg
     2101 ctcgaggagg aataccatga ccaagaggct gtacaagagc cggacagacc ggatgatcgc
     2161 cggggtgtgt ggcggcctgg cggagtactt cgacatcgac ccggtgctgg tgaggctgct
     2221 cttcctgctg ctggcgttcc tcaccggcac gggcttcatc atctatccca tcctctggat
     2281 cgtgatgccg gagcagacga gcctgcagcg accaccccgg gatgtggtct gggagaacat
     2341 tggcaagatg caggaggatg tcgaacggat cggggaagag atccacgccg gcaccagggg
     2401 gtccaccgtc gcgacggagg gatccgccac ggagggcacc acagtcgagg agcgcatcac
     2461 ggagaatggc ggcaggcagt cgaaggcggg ggaacccggg ctcgagggga ccgtcgaaga
     2521 tcgttcctac tacgacacgg agccctacaa tcgcccccac cgctcgcgaa ccgaccgcca
     2581 gttcatcggg ggggtgatcc tcctcgtcct cggtgtgatc ctgctgatgc agaacctggg
     2641 catattctgg tgggcaagcg tctggcggct gtggcccctg gtgctggtgg cgataggcgt
     2701 gtacctgctc tataagaggt ccaggccgcg actttaatga caggcgtgaa accgtagggg
     2761 cgacgcatgc gtcgccccta catctttact ccggcacgcg caccatcagc agcggttgct
     2821 ggatcctggc aaggatcttg gagccgacgg aggccgacca gaacgccccc aggccggcac
     2881 gaccgtgggt cgccatcact atcaagtcag ggtcgatctc tcggacgacg tcgacgaccg
     2941 agcttgcagc gtcgcctctc cgcacctcgg ccgacaccgt gatcttcttc tccatcatct
     3001 ccccggccaa tctagcaagg tattccctcc cctcgtcctc ctccatgtcc agtgcggctg
     3061 cagtagcgct ggggctgagg gtgttcaccg gcgcctgatt ccgcggcatg gtggaaagcg
     3121 tgggcacgac cagcagaaga tggagggagg agcccaaggc ctcggccacc cgctccacca
     3181 ccggcagcac gaccccatcg tgcgcctgct tcccgtccag gggaaccagt atcctcttcc
     3241 cctcgaacgg gggcgcaccg ccggagggcg tgggccttat caacaacacc ggaagcacgc
     3301 cacggcgcag tacctgctgg gctatgctgc ccaccagcag gtcccgcatg ccaccccatc
     3361 cgtgggtggc caggatgatg aggtcggcct cgaactctcg agcgtggccg atgatgctcg
     3421 ccacaacgtc gcgctcctgg tttggatgca cgtggatctc caccgaggag gcttggcagt
     3481 cgcactcgct cgccaccccg ttcagatact cctctgcctc cttgaggtta tcgagatggc
     3541 gctccccgtg gatggtctcc ggcgccccct gctccatgac gtggagcagg gacacggtgg
     3601 cgccgaaccg ctcggcaagg aggtgtgtgg cgggcaacac ggcctcggcc aggcgggatc
     3661 catccaacgg caccagcagc tttcggaagc ctttcattac gcgccacccc cgaagagtgt
     3721 ttggtatagc aggaagaggt tcaggacgac gatgagcgcc gcgaccaccg acgccacgac
     3781 cgtagtaagc cgtttgttca ccaggggacc catgatagcg cgattgcggg tgaacatgac
     3841 caacggtatc agcgcgaagg ggattgcgaa gctcagcacc acctggctca ccaccagggt
     3901 gcgggtaggg tcgagcccaa tcccggccac aatcagcgcc ggcaccatgg tgaacagccg
     3961 cctgaaccag agcgggatag tacggtgcag gaagccctgc atgatcacct gaccgctcat
     4021 ggtgccgacc gtggtggagg agaggcccga agccagcagc gacacggcga agatgacgct
     4081 ggaggcgctt cccaggagcg gggtcagggt cttgtgtgcc tcctcgatac tccccactcc
     4141 agaaatcccg tgggcattga aggtggaggc tgccattatt agcattgcgg cgttcaccag
     4201 cccggctatc cccatggcga tgaccacgtc caccacctcg aagtggaaca gccgtcgcag
     4261 tcgcgccggg tccctcacca cgattcgatc ctgggtgagg gcagagtgga ggaagatgac
     4321 gtggggcatt acggtggccc ccaggatgcc ggtggcgagc agaacggact ccgtgccgga
     4381 gaagcggggc tgcaccacgg cgaggccgac ctgtccccaa tcgggcgggc tgaagatcgt
     4441 ctccaggaga tagcaggcgg caatgatgcc caccatcacg gtgataaccg cctccagcgg
     4501 cctgaagccg tagcgctgga gacccaggat gatgaaggtg acgacaccgg tgatgaccgc
     4561 ggcgacgaag agggggatgc ccagcaacag gttgaagccg agggccgccc ccaggaattc
     4621 ggctagatcg gtggccatgg ccaccagttc ccccagcagc cacatgcccc atacgacggg
     4681 cctcgggtac cgctc
//
LOCUS       DATALS010000192         6131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_279438, whole genome shotgun sequence.
ACCESSION   DATALS010000192 DATALS010000000
VERSION     DATALS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279438"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..360
                     /locus_tag="VHS06_04230"
     CDS             40..360
                     /locus_tag="VHS06_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HEX2987361.1"
                     /translation="MRSAVAAATRDPRFQPVCARELPLLHYSVDILSNPEPVEELGQL
                     DPKRYGVIVECGSRGGLLLPDLEGVSTVEMQVEIARRKAGIPSGAEVTLRRFTVRRVS
                     EDIH"
     gene            complement(385..1509)
                     /locus_tag="VHS06_04235"
                     /pseudo
     CDS             complement(385..1509)
                     /locus_tag="VHS06_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
     gene            complement(1539..2258)
                     /gene="tatC"
                     /locus_tag="VHS06_04240"
     CDS             complement(1539..2258)
                     /gene="tatC"
                     /locus_tag="VHS06_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX2987362.1"
                     /translation="METGAREDLDAKRLTIIEHLEELRSRLIISVLALLIATTLSFVF
                     TGKLLEVLLIPSGGIKPVFLKPTEMFITYFKVALIGGAALAMPVIVYQLIQFVVPGLK
                     PEEKKYLFFVVPGATFLFVLGIAFGFVVLLPFALKYLLTFGGDIASAFISIGEYVSFV
                     VTLLLWMGLAFETPMIIVFLARLHVVTQQQLRGYWKYALVGSFVIAAIITPTPDPFNQ
                     SLVAVPLYLLYEVGVLFARFV"
     gene            complement(2424..2663)
                     /gene="acpP"
                     /locus_tag="VHS06_04245"
     CDS             complement(2424..2663)
                     /gene="acpP"
                     /locus_tag="VHS06_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX2987363.1"
                     /translation="MDAQTQEKLKKIVSEQLSVDLEEVVPEASFIEDLNADSLDLVEL
                     IMTMEEEFNIKISDEDAEKIQTVQDALDYLQEHLD"
     gene            complement(2696..3160)
                     /gene="nusB"
                     /locus_tag="VHS06_04250"
     CDS             complement(2696..3160)
                     /gene="nusB"
                     /locus_tag="VHS06_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX2987364.1"
                     /translation="MSASQRQLHGRRAARTLALQSLFEVDTTGHKPDEVLKRRTAEDG
                     LALDAAKYAEELVNGVLQHLSAIDELIRKAAPAWPFAQMAGIDRNIIRIALYESLFGE
                     ERVPFKVAVNEAVELAKKFGSESSGRFVNGVVGKIVSQEEAKREEGTDSTAG"
     gene            complement(3182..3520)
                     /locus_tag="VHS06_04255"
     CDS             complement(3182..3520)
                     /locus_tag="VHS06_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX2987365.1"
                     /translation="MTPEADFCSVRISPGVLATIAGLTTMSVPGVLRTSRGLVGGVSR
                     LVGRDDPTQGVKLRVKDELVYLDIHVVVEQGADLVRVGGQIQRDVAQAIDKMVGLPVA
                     EINVYVQDME"
     gene            complement(3538..4932)
                     /gene="accC"
                     /locus_tag="VHS06_04260"
     CDS             complement(3538..4932)
                     /gene="accC"
                     /locus_tag="VHS06_04260"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX2987366.1"
                     /translation="MFHKILIANRGEIAVRIIRACRELGVATVVAHSEADRHSLAVDL
                     ADEAICIGPATSDKSYLNISNVVSAALVSGAEAIHPGYGFLSENAYLAEVCEQCQITF
                     IGPTAKGIERFHDKSAAREMMREAGLPVLPGIDRPVYTLDAAQKAAAEIGYPVMVKAC
                     AGGGGRGLRPAFDETELARSFPVAQREAQSAFGNGGLYLEKLVEEARHVEVQVLVDRY
                     GKSVHFGERDCSLQRRHQKMLEESPCIALDSKVRAEMGERAAAAAAAVGYEGVGTIEF
                     LVDGAGRYSFMEMNTRIQVEHPVSEMTTGVDLLKWQMRVAAGEPLGFNQSDIAPRGHA
                     IECRVNAEDPARGFAPQSGLITGYVAPGGPGIRVDSHLYPGYQMPPYYDSLLAKVIAW
                     GEDRAEAVARMRRALDEFRLNGVATNIPLQLELIGSPEFLEGRFDTHFVERWLADREA
                     AVAEAGSAPGLHGE"
     gene            complement(4932..5369)
                     /locus_tag="VHS06_04265"
                     /pseudo
     CDS             complement(4932..5369)
                     /locus_tag="VHS06_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955749.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            5391..5684
                     /locus_tag="VHS06_04270"
     CDS             5391..5684
                     /locus_tag="VHS06_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987367.1"
                     /translation="MVIPPKIPLVLNASAGVSSDPEEIRHELVVQVTSPVRWSDSVKT
                     MLDAGCTVFVELGPGQVLGGLIKRVTKDVIILNVEDEDGLSRTASKLAEVLGR"
     gene            5824..>6131
                     /locus_tag="VHS06_04275"
     CDS             5824..>6131
                     /locus_tag="VHS06_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2987368.1"
                     /translation="MENAKVALITGGSRGIGRAIALRLARDGIAVVVNYVSSEAAALE
                     VVDAVLAAGGRAFSVQANVAIAVEAEAMVDRVVKEFGSLDILVNNAGITRDGLLLRMS
                     "
BASE COUNT         1217 a         2070 c         1738 g         1106 t
ORIGIN      
        1 acattcctgc cctccgagga gtctgtggcg gcggagatcg tgcgtagcgc cgtcgccgca
       61 gccacgcgcg atccgaggtt ccagccggtg tgcgctcggg agttgccgtt gctgcactac
      121 tcagtggata tcctctcgaa cccggagccg gttgaggagc tggggcagct ggacccgaag
      181 cggtacggtg tgattgtgga gtgtgggagc aggggtggat tgctgcttcc cgacctggag
      241 ggggtgtcca cggtggagat gcaggtggag atcgctcggc ggaaggctgg gatcccctcg
      301 ggggcggagg tgacgctgag gcgtttcacg gtccggaggg tgtcggagga tatccactga
      361 gtagtgcagc aacggcaact cccgagcgca caccggctgg aacctcggat cgcgcgtggc
      421 tgcggcgacg gcgctacgca cgatctccgc cgccacagac tcctcggagg gcaggaatgt
      481 gcctatgcag cctcgcaact cgcccccaac cttcaggcag acgaacacgc ccgcccggtc
      541 tcccagcacc ccagcgggca gggcaggcgg ctcgatcacc cggccctccc gaacgtaggc
      601 ttcaaccgcg gaccgcgcaa gcttcaggat ctcccgctcc tccgccctgc cctcaatacc
      661 ggacacgtcg ctcgcggtaa cgtcacagtc ggcctcctgg agctcctgag gcacaaatcg
      721 agcaaccaga taccccaccc cgaagggccc ttcgtaggag agcacttccg acttcgactt
      781 gggcagcgca ccaagagcga ttaccaggcc tcgataacca cactccccgg ccctgcaccg
      841 caacccctcg tccatcgaga taatgcccga gtagtccccc cgtcgaagga gtgcggcgag
      901 ggtctggtca aactggtccc cagcgggatc gaatccggcc ggggcaccgg ggcaaagcct
      961 gtgggacata tcgccactcg ccaccaggac gactcttctc cgggacgagc aggcggcggc
     1021 ggctatggct tggccgaagc gtaggtggga gttgacgtcg agagaggaga aagacaccag
     1081 ggtgagcgcg ctgttcaccc ctgcctcctg gaagaagtga agcggaacac acgcgccgtg
     1141 gtccaaccgg tgggttgcgc cggaccaact catcgccacc agcggtaccc ccctggcggc
     1201 gcactccgct ctcaaggcag acaccaggga ggggtctgct tcagcagata ccctcaccga
     1261 tggagcacca aaatcagcga agctgccact gtagccgccg gcaaccgcga cgctcatgga
     1321 ccgaggatcc accggagcat ggggcgaaat caacagcagg gtatcgggag caagctcccg
     1381 aacccctgcg gccagacagc gcatcgcctc caggctttgc cgcacctgct cgacgcgccg
     1441 tccgccaaca gcgggcacca agatcggtgg atgtggagtc aagcagccaa gtattacacc
     1501 gacgcccatc acatcagctc ctcttgggag ccaccaccct acacaaaccg ggcgaacagc
     1561 accccaacct catagagaag gtagagcggc accgcaacca ggctctggtt gaatgggtcg
     1621 ggagtaggcg tgatgatagc cgcaatgacg aaggagccaa ccaaagcgta cttccagtaa
     1681 cctcgcagct gctgctgtgt cacgacatga agcctggcca ggaagacgat gatcatgggc
     1741 gtctcaaagg caagccccat ccagagcaac aacgtcacca cgaaggaaac gtactcgccg
     1801 atgctgatga aggcgctggc tatatcccca ccgaaggtca gcagatactt cagggcgaat
     1861 gggagcagca ccacgaagcc aaaggcgata ccaaggacaa acaggaaagt cgcaccgggc
     1921 accacaaaga acagatactt cttctcctcc ggcttcaggc ccggtaccac aaactggatc
     1981 aactgataca cgatcaccgg catcgccagc gccgcaccac cgatcagtgc caccttgaag
     2041 taggtgatga acatctcggt gggcttcagg aataccggct tgatcccacc cgaggggatc
     2101 agcaggacct ccaacagctt tccagtaaag acgaacgaga gggtcgtagc gatcagaagg
     2161 gctaagaccg agataatcag cctcgatcgc agctcctcca gatgctcaat tatcgtgagt
     2221 ctcttggcgt cgagatcttc cctagcaccg gtttccatgg cgtgatgata gcctcgtttc
     2281 ctattgcctt agctcactcc actactgccc ttcccgtcac cggtccaccg gagcaccgat
     2341 gagcgagggc cgtccgcaac ccaagagaga cgaacgtggc gactgccgga atcctgaccg
     2401 accagaatag cggctctgcc ggtctagtct aggtgctcct gcagatagtc cagcgcgtcc
     2461 tggaccgtct ggatcttctc cgcgtcctcg tcggagatct tgatgttgaa ctcctcttcc
     2521 atggtcataa tcagctcaac cagatcgagg gaatccgcat tcaggtcctc gataaacgag
     2581 gcctccggaa caacctcttc caagtcaacg ctgagctgct ccgaaacgat cttcttcagt
     2641 ttctcctgtg tctgtgcatc cagagccacc ctgtaccctc cttccttccc ggcctctagc
     2701 cggctgtcga gtctgtgccc tcttccctct tcgcctcctc ctgggagacg atcttcccaa
     2761 caacaccgtt cacgaaccgg cctgaactct cactgccgaa cttcttcgcc agttccacgg
     2821 cttcgttcac ggcaaccttg aaggggaccc gctcctctcc gaaaagcgat tcgtagaggg
     2881 ctattcgaat gatattccga tcgatgcccg ccatctgggc aaacggccac gcgggggcag
     2941 ccttcctgat gagttcgtct atggcggaga ggtgctggag aaccccgttc accaactcct
     3001 cggcatactt ggctgcgtcg agcgccagcc catcctccgc ggtacgccgc ttcagaacct
     3061 catccggctt gtggccggtg gtgtccacct caaagaggct ctgaagcgcc aatgtgcggg
     3121 cggctcgccg cccgtgcagc tgtcgctgcg aagctgacat cgctctttcc ccgccgagcc
     3181 gctactccat gtcctgaacg tacacgttaa tctcggccac cggcaatcca accatcttgt
     3241 cgatcgcctg cgccacgtcc cgctggatct ggccgccaac cctcaccaga tcggcaccct
     3301 gctccaccac cacgtgtatg tccaggtata ccagctcgtc cttgactctc aatttaacgc
     3361 cctgggtggg atcatcccgc ccaaccagcc tactgacccc gcccaccagg cccctgctgg
     3421 tcctcagcac cccgggtacc gacatcgtgg tcaggcccgc aatggtggcc agaacgccag
     3481 gcgagatcct tactgaacag aagtctgcct ccggagtcat tgtaccctct cccagtacta
     3541 ctccccgtgc aaccccggcg cgctgccggc ctcagccact gcagcttccc gatcagccag
     3601 ccatcgctcg acgaagtggg tatcgaatcg cccctccagg aactcagggc tgccaatcaa
     3661 ctccaactgg agcggaatgt tggtggccac accattcagc ctgaactcat ccaacgcccg
     3721 cctcatccga gccacagcct ctgcccgatc ctccccccag gctatcacct tcgccaacag
     3781 cgagtcgtaa tacggcggca tctgatagcc ggggtacaga tgggagtcca ccctgatccc
     3841 aggccctccc ggtgctacat agcccgtgat caatccggat tgcggcgcaa accccctggc
     3901 cggatcctcg gcgttcaccc tgcactctat ggcatgtccc cggggcgcta tgtccgactg
     3961 attgaacccc agcggctccc ccgcggccac ccgcatctgc cattttagca ggtctacgcc
     4021 cgtggtcatc tccgaaacgg ggtgttccac ctgaatccgc gtgttcatct ccatgaaact
     4081 gtagcgcccg gcgccgtcca ccaggaattc tatggtccca accccctcgt agcccaccgc
     4141 ggcagcagcg gctgccgccc gctcgcccat ctcagccctc accttagaat ccagggcgat
     4201 acagggggat tcctctagca tcttctggtg ccgtcgctgc agggagcaat cccgctcgcc
     4261 aaaatgcacc gatttgccgt acctgtccac cagaacctgg acctcgacgt ggcgagcttc
     4321 ctcgaccagc ttctccagat acagcccgcc gttgccgaag gcgctctgcg cctctcgctg
     4381 ggccacaggg aagcttcggg ccagctcggt ctcgtcgaaa gccggtctaa ggccccgccc
     4441 accaccgccg gcacacgcct taaccatgac cggataaccg atctcggcgg cggccttctg
     4501 ggcagcatcc agagtgtaca ccggcctgtc aatccccggc agcaccggca acccggcctc
     4561 tctcatcatc tcccgagccg cggacttatc gtgaaaacgc tcgatgccct tcgccgttgg
     4621 cccgatgaaa gtgatctggc actgctcgca cacctctgcc agatatgcat tctcagacag
     4681 gaaaccgtag ccgggatgga tggcctccgc gccgctcacc agtgccgcgc tcaccacgtt
     4741 ggagatgttg aggtagctct tgtcggatgt ggctggacct atgcagatgg cctcatctgc
     4801 cagatccacc gctaaggagt ggcggtccgc ctcagaatgt gcgaccacgg tcgccacccc
     4861 gagttcccgg caggccctga tgatccgcac ggcgatttcg ccccgattgg cgataaggat
     4921 cttgtggaac actagctcat caccattccg ccgtccacgg agagcacctg acccgtcata
     4981 tagccggagg catccgaggc gaggaaggcc accacctcgg ccacatcctc gggttgccct
     5041 gccctggcca ttgggatcaa ctgcagcagc cccgcccggg cctgctccgg aacaccgttc
     5101 cagatgtcag tctcaatgta gccgggagcc acggcattga ccgtgatgtt gcggctggca
     5161 acctctcgcg ccaccgcacg ggtgaagccg atcaatcccg ccttggaggc ggaatagttg
     5221 gcctggccgg cgttccccat aagccccgcc acggaactca cgttcacgat gcgaccgtac
     5281 ttccggcgca tcatgaagcg tatcgccgcc ttcgtgcagg cgaaggctcc cttgagattg
     5341 gtgtccagca ccgcatccca gtccgcttcc gcgcacccgt ggcggaggcg atggtcatcc
     5401 cccctaagat cccattggtt ctcaacgcca gcgccggcgt ctccagtgat ccggaggaga
     5461 tccggcacga actggtggtc caggtgacca gcccggttcg atggtcggac tctgtgaaga
     5521 ccatgctgga cgccggatgt accgtcttcg tggagttggg tcccggacag gttctgggcg
     5581 gattgatcaa gcgagtcacc aaagacgtta taatcctcaa cgtcgaggac gaagacggcc
     5641 tcagcagaac cgcgagtaag ctcgccgagg tgctgggccg ctaaaggcgg ctctctggta
     5701 gcggggtgac tctccatctc gccgtcgcag gagatgaacg acggcgagcc gcgtttatgt
     5761 ctccgccgga cacccccaaa atccatcacc ctttgggtgt ttcgacccgg gtgggaggtt
     5821 ctcttggaaa acgcaaaggt cgctctgatc accggtggat cccgcggtat aggcagggcc
     5881 atcgccttgc gcctagctcg tgatggcatc gccgtggtgg tgaactacgt ctccagcgag
     5941 gcggccgccc tcgaggtggt ggatgcggtg ttggcagcgg gtgggcgagc ctttagcgtc
     6001 caggccaacg tcgccattgc cgttgaggca gaggcgatgg tggatcgtgt ggtaaaggag
     6061 ttcggcagcc tggatatcct ggtcaacaac gccgggatca ccagggatgg tttgctgctg
     6121 cggatgtcgg a
//
LOCUS       DATALS010000193         3599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_20029,
            whole genome shotgun sequence.
ACCESSION   DATALS010000193 DATALS010000000
VERSION     DATALS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20029"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            514..1851
                     /locus_tag="VHS06_04280"
     CDS             514..1851
                     /locus_tag="VHS06_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2987369.1"
                     /translation="MEDLEHLFLDFMQMREFARDPFLFAEGDGIRVTDVQGKQYIDGL
                     SGVFVNSLGYRNQAVIEAVTAQMQRLHFAPPLHGSTPQALALAKELLEMAPAGMGAVK
                     FLSGGSEATETAMKMSRQYHRQTGHPRKFKIIARYGGYHGATMGALSASGGWERKSVF
                     EPLVAGFLHVHPPYCYRCPYDKSYPGCGITCARVVERTIQAEDPETVAAVIMEPMSIS
                     AAGFVVPPREFFAQLRDCCDRHGVLLIFDEVITGFGRLGTNFGADYYGVAPDLLCCGK
                     GMSGGYVPLAAVIISERVQSAFLGRGEDRVEFHHGHTFGGNPVACAAGLAAMAQIRDR
                     NLAENSREMGGHLRARLEAMAERFSFIGEVRGDGLLQGMDFVTDRQTREPFPPKVKPG
                     KLLERAAKERGLIMRTGNDFAALAPPLIVNRTDVDEMCDILEASLAAIAPAFV"
     gene            complement(1848..3089)
                     /locus_tag="VHS06_04285"
     CDS             complement(1848..3089)
                     /locus_tag="VHS06_04285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2987370.1"
                     /translation="MKDSLPRFRWVALALAMLSQTSSSFILSVLTPIVPLFQSDLGIS
                     KAEVGLLFSATSVGAWSVVLLAGYLTDRFGIRKVMSTALMVAGSMLLCMAAVGSFVQA
                     MVVMFVAGMFRGPVFPSSTKAVLTWFPPQARATAMGVKQMGLPVSGVVAATILPGLGL
                     ALGWRSAVAIPGLLIILAGVTAAVFYRAAPGEQTMARSSVGFKQAMGVMIRNRMLLMV
                     CLLCFCFLVVQLAMISYLAVYFKEVALLDTFPDESSRIIAAGGFLAVCQIGGAIARVG
                     WGAMSDRLFHGRRAVVLAIVGALTMSMSVLFASLGPGHPLWALGLSAFCFGVTAVGWN
                     GVYNALLTETVGSQYAATGVGLTMTLTELGTISGPPLFGLVLDVTASYQVAWLFLAVM
                     AAIGTLAALQVTRMEKPVISR"
BASE COUNT          754 a         1105 c         1140 g          600 t
ORIGIN      
        1 ccctctttca actggccggt aatcatggtt cgcctgcgca aaacgatgga cgagcgtgtg
       61 cggtttggac gtgcttcata gtgcacatcg gattgcgata atgaatttgc ccaacggtga
      121 tgttccgcgc atatgtcgat gatcgtcatg ggttgtcaga agcaatgagt gatcattact
      181 tgattctgct gcaataagga cttcttcccg ctttgagcta tactatgagc agaacatagg
      241 caaccaagca tggagtcgat tcggccatca taatcattcc tgtccacgtc ttgaggtgag
      301 tgctatggaa acaagcaatg atgacaaggg ccgagccggt acaagacgtg gtgcaccgtc
      361 cgaacagata cgcccattga cgcccactcc caatccccta caattgatca tgttgtatat
      421 gggtatagat gctcctaagt gaacgggaag ccaagacagg acctgctcac tgggccataa
      481 tctgaccgac attcatatat ggagggccgg ctgatggagg atctggagca tctcttcctg
      541 gacttcatgc agatgcggga gttcgcccgc gacccattcc tcttcgccga gggcgacggc
      601 atccgcgtca cggacgtgca gggcaagcag tacatcgacg gactctccgg ggtgttcgtc
      661 aacagcctgg ggtatcgaaa ccaggcagta atcgaggccg tgaccgcgca gatgcagcgg
      721 ctccacttcg cccctcccct ccacggctcg accccgcagg ccctcgccct cgccaaggaa
      781 ctgctggaga tggcacccgc cggaatgggg gcggtcaagt tcctgagcgg tggctccgag
      841 gcaaccgaaa ccgccatgaa gatgtcccgc cagtaccacc ggcagacggg ccacccccgc
      901 aagttcaaga tcatcgccag gtacggcggg taccacggcg caaccatggg cgccctctcc
      961 gccagcggcg gctgggagag gaaatcggtc ttcgagccgc tggtggccgg cttcctccac
     1021 gtccaccctc cttactgcta tcggtgtccc tacgacaaga gctacccagg ttgcgggatc
     1081 acctgcgccc gagtggtgga gcgcacgatc caggcggagg acccggagac ggtggccgcc
     1141 gtcatcatgg agccgatgtc gatctctgcc gcaggtttcg tcgtcccgcc gagggagttc
     1201 tttgcacagc tacgcgactg ctgcgaccgc cacggcgtgc tgctgatctt cgacgaggtg
     1261 atcaccgggt tcggccggct cggcaccaac ttcggcgcgg actactacgg cgtcgcgccg
     1321 gatctgctct gctgcggcaa ggggatgagc ggcggctatg tcccgctggc ggcggtgatc
     1381 atttccgagc gggtgcagag cgccttcctg ggcagggggg aggatcgagt ggagttccac
     1441 cacggccaca ccttcggtgg taacccggtg gcctgcgccg cagggctggc ggcgatggcg
     1501 cagatccgcg accgcaacct ggcagagaac tccagggaga tgggtggcca ccttagggcg
     1561 aggctggagg cgatggctga gcgcttcagc ttcatcggcg aggtgcgggg cgacgggctg
     1621 ctgcagggga tggatttcgt cacggacagg caaacccgcg agccgttccc gccgaaggtg
     1681 aaaccgggca aactgctgga gcgagcggcc aaggagcggg ggctgatcat gcgcaccgga
     1741 aacgacttcg ccgccctggc cccgcccctc atcgtgaacc gcacggacgt ggacgagatg
     1801 tgcgacatcc tggaggcgtc gctggccgcc atcgcgcccg cgttcgtcta acgggatatc
     1861 accggcttct ccatccgggt cacctggagg gcggcgaggg tgccgattgc ggccatcacg
     1921 gccaggaaga gccaggcgac ctggtatgag gcggtgacgt ccagaaccag tccgaagagt
     1981 ggggggccgg atatcgtccc aagctcggtc agggtcatgg tcagcccgac cccggtggcc
     2041 gcatactggc tccccaccgt ctcggtcagc agcgcgttgt agacgccgtt ccatccgacc
     2101 gcggtgacac caaaacagaa ggcggagaga cccagcgccc agagggggtg gccggggcct
     2161 agggaagcga acagcaccga catcgacatg gtgagcgcgc ccactatcgc cagcaccaca
     2221 gccctccgtc cgtggaacag ccgatcgctc attgcccccc agcctaccct tgcgatggcg
     2281 cccccaatct ggcatacggc caggaatccg cccgcggcga tgattcgcga ggactcatcg
     2341 gggaaggtgt ccagtagcgc aacctccttg aagtagacag ccaggtagct gatcatcgca
     2401 agctggacca ccaggaaaca gaagcatagg aggcagacca tcagcagcat cctgttgcgg
     2461 atcatcaccc ccatcgcctg cttgaagcca acgctgctcc gggccatcgt ctgctcgccg
     2521 ggcgcggctc ggtagaagac cgcggctgtg acacccgcca ggatgatgag gagaccgggg
     2581 atggccaccg cggagcgcca accaagggca agccccaacc ccggcagtat cgtggccgcc
     2641 accaccccgg aaaccggcag ccccatctgc ttgactccca tggccgtcgc cctcgcctgt
     2701 ggtgggaacc aggtaaggac ggccttggtg gagctgggaa agaccggccc tcggaacatc
     2761 cccgccacga acatgacaac cattgcctgg acgaaagaac caacggccgc catgcagagc
     2821 agcatcgagc cggcaaccat cagcgccgtg gacatcacct tacggatacc gaaacgatcg
     2881 gtgagatagc cggccagcag taccacgctc caggcgccga cggaggtagc cgaaaagagc
     2941 agccccacct ccgccttgga gatgcccaga tcggactgga agaggggtac gattggggtt
     3001 aagacagaca gaatgaacga tgaactggtc tgcgagagca tggcgagcgc cagggcaacc
     3061 catcgaaatc tgggaagaga gtccttcaaa gtggggatca caactccttg tagagctcgt
     3121 cggccggggg gagactccag aagggtgacc gcacggctgg ctagacgatt gtacaacaag
     3181 tcatctccga gcagaaccgg accgagttgc cggactgtac gtagactctt tgttcacgac
     3241 ccacaagctg gtatcatcaa gtgccccagg accaaggaca agaagtcgcc gcaggacggc
     3301 accgacaacc gccgccggct tcatcgctag tgctcgacca cagttattct ggaaggtgtc
     3361 atcctgagcg acagcgaagg atctctccgt tcacgaggag atgcttcgct tcgctcagca
     3421 tgacagtacg tgtcacctct actgtggcgg agcactagag atctatccgg cgagccatgc
     3481 gaggagggga tcaagtgggt cattgggttg cactggccgg ccagcagggg gggcaatgag
     3541 cgatcggctg ttccacggac ggagggctgt ggtgctggcg atagtgggcg cgctcacca
//
LOCUS       DATALS010000194        10925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_201323, whole genome shotgun sequence.
ACCESSION   DATALS010000194 DATALS010000000
VERSION     DATALS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201323"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VHS06_04290"
     CDS             complement(<1..663)
                     /locus_tag="VHS06_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987371.1"
                     /translation="MRRLRLWLALALAMSLTVLATSANAAPGDLDYDIANGHFFTQTN
                     GQPLGTSTAGYSITNDAGIPFWDEFQKLGGAQVLGYPVSKRFQYDGYVTQAMQKVVFQ
                     WRPDYKEVWFLNTFDALQDKGKNDWLLNYRQTPKTMDTTPDNGLTIEQVIRRHIGFLD
                     QNEAIKKVYNSDKQSIDHYGLPVAYADMGNSFVIRAQRATFQYWKEDVPWANKGDVTV
                     ANG"
     gene            1018..1485
                     /gene="greA"
                     /locus_tag="VHS06_04295"
     CDS             1018..1485
                     /gene="greA"
                     /locus_tag="VHS06_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX2987372.1"
                     /translation="MEKTVYLTREGFAKLEEELNHLRTVRRHEVAERIRKAKEFTNTV
                     DNAEYEDAKNEQSFVEGKIQSLERMLANAVVIDDKAAPSGYVRVGSHVTVTDSDGIEE
                     SYAIVGSAEADPRQGRISNESPIGRALLGKKVGETVSVMAPGGAFDLVIKSVS"
     gene            1615..3078
                     /gene="lysS"
                     /locus_tag="VHS06_04300"
     CDS             1615..3078
                     /gene="lysS"
                     /locus_tag="VHS06_04300"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX2987373.1"
                     /translation="MDSQIDPLVEQRIAKVKELREAGINPYPYRFDRTHLASQVVERF
                     DALEGTEVRVAGRMLTSRIMGKASFAHIQDQSGRIQIYVRRDLVGEEQYDLFKSLDSG
                     DFVGVGGKPFRTRTGEITVEAQQITLLCKALRGLPEKWHGLTDTEKRYRQRYLDLISN
                     DEVRVVFQQRSRIIDAIRRYLVDKGFLEVETPILQPIHGGAAARPFVTHHNALDRDLY
                     LRIALELYLKRCIIGGIEKVFEIGRNFRNEGISTRHNPEFTMMEVYQAYADYNDIMEL
                     VEQMVSTVAEQVLGQTTVEYNGHTIDFRPPWPRVEMRDAIRIESGIDYEDYPTADALW
                     AAMREKGVHADPNSSRAKLIDDLLSAFVEPKLVQPTFLIDYPVEMSPLAKRKRNDPSV
                     VERFEAYVGGMEIANAFSELNDPLEQRERFVQQMAERAAGDEETQPMDEDFLLAVEHG
                     MPPTGGLGVGIDRLVMLLTGNSSIREVILFPQLRTRE"
     gene            3087..5303
                     /locus_tag="VHS06_04305"
     CDS             3087..5303
                     /locus_tag="VHS06_04305"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEX2987374.1"
                     /translation="MRHPLYVAFVWHMHQPYYLDEGSGELLLPWVRLHATKDYLHMAE
                     VIEDYPQVHATFNFVPSLVEQLQGYVDGTSSDRAWRVSLKEKLTREDKEFVDNLFYSV
                     NRDNFIRSYPRYWQLLRLKEEAGGDLDLLGELYWRDLVAWFNLAWIDPSWIQRDPELR
                     ALVAKGSHFSLEDVSVILGKHRELMGATLTAFRHLQRRGQVELIVSPYYHPILPLLID
                     SNCAQEPSPWLQLPTERFVQRDDAMEQLRRAIAHHEGVFEKPPSGLWPSEGSVSQSLI
                     DLLGGIPEIRWLATDEAILARSLGQNIERNGWGYLSDPRFLYQPYGVQAGDRRVAMVF
                     RDHYLSDRIGFAYQGMTGRQAAEDLMFRFVAIHEMVKDDPEPYLVPIILDGENCWEYY
                     EKNGETFLRALYEMMSNQDRVRAVTVSEYLNEFPVRREIPRLATGSWINGNLETWIGE
                     DTQNRGWEYLYRTRAALAEAESRDDIPDLKLQRARRSMMAAEGSDWFWWYYSHNVSDQ
                     DAMFDELYRNFLAGVYRALDRPVPDWISIPIITQVPKLTSRKPRGYIRPQLSKTDLSP
                     APWDLAGYLEPKGSTGAMQQGSGLLKRLYFGYDDSKLYLRLDCHSSLAGYIVELYMSH
                     LEGISSVEGGEPSQGAEIRASAAAASRDLSIDAAARSAGLSLWQEGSGWLGIDGKLLL
                     MVTENVVELAVPLEQLGVGLGARLGLSVVLFREGLVVQRLPEEGEVVVDLALVELE"
     gene            5414..6697
                     /gene="serS"
                     /locus_tag="VHS06_04310"
     CDS             5414..6697
                     /gene="serS"
                     /locus_tag="VHS06_04310"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003392668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX2987375.1"
                     /translation="MLSLQFIRDHADVVRESLRKRHESAPVEEVLELDVRRRQLLQQL
                     ESMRAEQNRASAEVAKAKDKSAVADRIAELRQLSQRIKEMEPTIKEIEERLGQLLLLI
                     PNIPDDSVPEGKDDSENVVIRTWGEPRKFDFSPLPHWEIGERLGIMDFERAAKISGAR
                     FGVLKGLGAKLERALISFMLDLHTTEHGYTEVLPPYLVRRECMVGTGQLPKFEEDAYR
                     TEPDDLFLVPTAEVPVTNLHRDEILDAAALPIYYAAYTACFRREAGSAGRDTRGLIRV
                     HQFDKVELVKFVAPETSYDELEKLVGDAEDVLKRLGLPYHVAMMCTGDVGFTAAKKYD
                     PEVWMPGQNKYVEISSCSCFTDFQARRANIRYRPEAGGKTEFVHTLNGSGLAIGRSMA
                     AILENYQQADGSVTIPEALRPYMGGLEALVPPARA"
     gene            7368..8708
                     /locus_tag="VHS06_04315"
     CDS             7368..8708
                     /locus_tag="VHS06_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX2987376.1"
                     /translation="MARAEARVSGSASGNKETAGDSSATRVLAGQDRIREWQEDVYRT
                     LHQHPELSNQELNTAATAAGALREAGYEVHDKIGTTGVVGILRNGDGPTVLMRADMDA
                     LPMKEETGLPYASTDHQTDGSGQVVPVAHSCGHDVHVASLMGAARLLAANRSAWHGTF
                     VPLFQPAEEVGTGADEMVKGGLATLIPKPDVALAQHVLAYPAGTVGTLSGPFLSTAAS
                     MRVTVHGRGSHGSMPQLSIDPVVLASMIVVRLQAIVAREVAPDDFAVLTVGQVAAGTK
                     SNIIPDHAVIELNIRAYSDSTRTLLIDAVKRVVQGECMASGSPKDPEFELYDEYPLTS
                     NNPEVTAKVAAAFKGYFGNRAFTAPRQSASEDFSRIPAAFGVPYTYWALGGIDDTKWH
                     KAEAAGRIASDIPANHSPKFAPVIEPTLKTGTEAIVVAALAWLSSPAGVPRERP"
     gene            8830..10245
                     /locus_tag="VHS06_04320"
     CDS             8830..10245
                     /locus_tag="VHS06_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014292952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2987377.1"
                     /translation="MILGVLAFWLFAQTTLNIAPTMAADLGIAMNVMNIAVSITALFS
                     GIFIVVMGGLADRVGRVKVVQWGFVLSIVGSLLVGVTPSGALASTILVLGRICQGLSG
                     ACIMPASLALVKAYWEGAGRQRAVSLWSIGSWGGSGFAPLFGGLMAENVGWRWIFFSS
                     AAVSVIGMLMVRGTPESRAEAKGDYRFDTVGVLTFMIAMVALQVFVTQGAKLGWTSAA
                     ALGLLGMSLLFGIIFFRAESGNANAFVDFRLFRNRIYTGSTLSNLLLNGVAGILIVAM
                     TLVQIGGGMSAQGAGLLTLGYAIAIVAFIRVGEKLLQRFGPRKPMIWGSLIVGVSILL
                     LMPTYVLLGTYKILAIIAFTLFGIGLGFYATPSTDAALASLPDDEAGSGSGIYKMASS
                     LGGSFGVAISATIFTALSADNAPVNWISGVITFVGRQDNLALREAAFFALCANLLMVA
                     AAIISIVLTVPKGRMSEETRK"
     gene            10271..>10925
                     /locus_tag="VHS06_04325"
     CDS             10271..>10925
                     /locus_tag="VHS06_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX2987378.1"
                     /translation="METLVLALDLVGTFVFALSGAMTSVRHRLDLFGVFVLSFAAGNA
                     GGITRDLLIGAVPPAAIDDWRYIVVSLLAGLATFRWHPTVRRLQSLVLVFDAAGLALF
                     AVSGALKALAFHLNPLAAVLLGVVTGTGGGAMRDVLVAEIPTVLRAELYAVAALVGAT
                     VVVGGQLLQFPSTPLAIVGAVLCFGLRLLAIRRGWGLPVARLPEQPTDRSSLIPCGPP
                     "
BASE COUNT         2027 a         3153 c         3556 g         2189 t
ORIGIN      
        1 gccgttggcg acggtgacgt cgcccttgtt tgcccacggc acgtcttcct tccagtactg
       61 gaaggtagct cgctgggctc ggatcacgaa ggagttgccc atatctgcgt aggcgacggg
      121 cagcccgtag tggtcgatgg actgcttgtc cgagttgtaa accttcttga tagcttcgtt
      181 ctggtcgagg aatccgatgt gacgcctgat gacctgctcg atggtgaggc cgttgtcagg
      241 cgtggtatcc atcgtcttgg gcgtctgcct gtagttgagg agccaatcgt tcttgccctt
      301 atcctgcagg gcgtcgaagg tgttcaggaa ccagacttcc ttgtagtcgg gtcgccactg
      361 aaagaccacc ttctgcattg cctgggtcac atagccgtcg tactggaacc gcttggaaac
      421 ggggtagcca agcacctgag ccccgccgag cttctggaac tcatcccaga atgggatgcc
      481 agcgtcgttc gtgatggagt agccggcggt gctggtacca agaggttgac cattggtttg
      541 agtgaagaag tgaccattgg cgatgtcgta gtcgaggtcc ccgggagcag cattggccga
      601 ggtcgccagg accgtcaacg acatggccag tgccagagcg agccagagtc gcagtcttct
      661 caaccttccc tccacactga ttggaaatcg agaggcgtgt tcccgatggg cgtgccgtcg
      721 gcaggctgga tcgacggggt gatcagttga agcggcggca gttctccccg cacagggggg
      781 aagaacgtgg taattatagg ttcgggttgc acaactgaca agcagttgct gcatcaagtt
      841 ggggtggctt tgtgggcttc gcaggaggaa agctctttac aatactgtat gaatcgcgta
      901 taatcacatt accgcaacag actgagggcc cctgtgaccc tcagtcttta ctaattcccg
      961 ggtcctcggg tgaccgtccc agcgcgcacc tcggtgaacg caaacaggag acttgccgtg
     1021 gagaagaccg tttaccttac acgtgaaggg tttgccaagt tggaggagga gctgaatcac
     1081 ctccgcaccg ttcgtcgtca cgaggtagcg gagcgcatcc gaaaggcgaa ggaattcacc
     1141 aacacggtgg ataacgccga gtacgaggat gcgaagaacg agcaatcttt cgtcgagggc
     1201 aagatccaat cgctggagcg tatgctggcg aacgcggtgg tgatcgacga caaggcagca
     1261 ccttccggct atgtgcgggt tggctcccac gtaacggtga cggactcaga tgggatcgaa
     1321 gagagctacg ccattgtggg ttctgcagag gcggatccgc ggcagggccg gatttccaac
     1381 gagtcgccga tcggtcgagc tctgctgggg aagaaggtcg gcgagactgt ctcggtgatg
     1441 gcacctgggg gcgccttcga tctggtaatc aagtcagtgt cgtagggttg tctggctgcc
     1501 aacgccaact atgtcgagca ctgtatccga aaatgcaaat cgtgcgcact acaggggaag
     1561 gatggcttgt ccatccttct ctttgcatag ggcgcggagt ggaaggaact tggtgtggat
     1621 tcgcagatag atcccctcgt tgagcagcgc attgcaaagg taaaggagct acgagaggca
     1681 gggattaacc cctaccctta tcgcttcgat cgaacccacc tagcctctca ggtggtggag
     1741 aggtttgacg cactggaggg tacagaggtt cgtgtcgccg gccggatgct aacgtccagg
     1801 atcatgggca aggcgagctt cgcccatatt caggatcagt caggccgaat ccagatctac
     1861 gtgcgtcgcg acctggtggg cgaggaacag tacgatctgt ttaagtcgct ggatagcggc
     1921 gacttcgtcg gagtgggcgg caagcccttc cgcactcgca ccggtgagat aacggtggag
     1981 gcgcagcaga tcactctact ctgcaaggcg ctgaggggac tccccgagaa gtggcatgga
     2041 ctgaccgaca cggagaagcg gtaccgacag cgatacctgg acctgatctc gaatgacgag
     2101 gtccgggtcg ttttccagca gcgcagtcgc atcatcgacg cgatccgccg atacctggtg
     2161 gataagggct tcctggaggt cgagactccg atccttcagc cgatccacgg cggcgccgca
     2221 gctcggccct ttgtcaccca ccacaatgcg ctggacaggg acctgtatct gcggattgcg
     2281 ctggagttgt acctgaagcg gtgcatcatc ggtgggatcg agaaggtgtt cgagatcggc
     2341 cgcaacttcc gcaacgaggg catctctacc cggcataacc cggagttcac catgatggag
     2401 gtgtatcagg cgtatgccga ctacaatgac atcatggaac ttgtggagca gatggtatct
     2461 accgtggcgg agcaggtgct ggggcagacg acggtggagt acaacggcca caccatagat
     2521 ttccgtcctc cctggccccg tgtggagatg cgggatgcga tccgcatcga gagcgggatc
     2581 gactacgagg actatccgac ggccgatgcg ctctgggccg ccatgcgcga gaagggggtt
     2641 catgccgacc ccaacagttc cagggcgaag ctgatcgacg accttctgag cgccttcgtg
     2701 gagccaaagc ttgtccagcc caccttcttg atcgactatc cggtggagat gtcgccgctg
     2761 gcgaagcgca agaggaatga cccgtccgtg gtggagcggt tcgaggcgta cgtagggggc
     2821 atggagattg ccaacgcgtt cagtgaattg aatgatcctc tagagcagag ggaacgcttc
     2881 gttcaacaga tggcagagag ggctgccggt gacgaggaga cacagcccat ggacgaggac
     2941 ttccttctag cagtggaaca tggaatgcca cccaccggtg gcctgggagt cggcatcgat
     3001 cgtctggtca tgctgcttac aggcaatagt tccattcgcg aagtcattct cttcccgcag
     3061 ctgaggacca gggaataggt cagtctatgc gacacccgct ctacgttgcc ttcgtctggc
     3121 acatgcacca gccctactat ctggacgagg gcagcgggga gctgttgctg ccatgggtcc
     3181 ggctccacgc caccaaggat tacctccaca tggcggaggt aatcgaggac tatccccagg
     3241 tccatgccac gttcaacttc gtcccctccc tggtggaaca gcttcaggga tacgtggatg
     3301 ggacaagctc cgaccgagca tggcgggtca gcttgaagga gaagctgact cgcgaggata
     3361 aggagttcgt cgacaacctc ttctacagcg tcaaccgcga caacttcatc cgcagctacc
     3421 ctcgctactg gcagttgctg aggttgaagg aagaggccgg cggggatctg gatctgctgg
     3481 gtgagctgta ctggcgagac ctcgtcgcct ggttcaacct tgcatggatc gatccgagct
     3541 ggatccagcg cgatcctgag ctgcgcgcct tggtggcaaa gggttcccac ttctccctgg
     3601 aggacgtcag cgtgatcctg gggaagcaca gggaactgat gggtgccacc ctcacagcgt
     3661 tccgccatct ccaacggcga ggccaggtgg agctaatcgt ctccccctac taccatccta
     3721 tccttccgct cctgattgac tccaactgtg ctcaggagcc gagcccctgg ctccagcttc
     3781 ccaccgagcg cttcgtacag cgtgatgacg ctatggagca gttgagacgg gccatcgccc
     3841 atcacgaggg tgtcttcgag aagccgccct ccggcctatg gccatccgaa ggctcggtga
     3901 gccagtcgct gatcgacctt ttaggaggga tccctgagat ccgatggctg gctaccgacg
     3961 aggcaattct ggctcgatcc ctcgggcaga acatcgagcg caatggctgg ggctatctct
     4021 cagatccccg cttcctctat caaccctatg gtgtgcaggc cggcgatcgc cgtgttgcca
     4081 tggtcttccg cgaccactat ctctcggaca ggatcgggtt tgcctaccag gggatgacgg
     4141 ggcggcaggc ggccgaggac ctgatgttcc ggttcgttgc catccacgag atggtcaagg
     4201 atgaccctga accttatctc gtgcctatca ttctggatgg ggagaactgc tgggagtact
     4261 acgagaagaa cggcgagacc tttctgcggg cactctacga gatgatgtcc aaccaggatc
     4321 gggtgcgcgc ggtcaccgtc tccgagtatc tcaacgagtt cccggttcga agggagatcc
     4381 ccagactggc gaccggctcc tggattaacg gtaatctgga gacgtggatc ggcgaggaca
     4441 ctcagaatag gggttgggag tacctgtatc gcacaagggc agcgctggcg gaggccgaga
     4501 gtcgagatga tatcccagac cttaagctgc agagggcacg ccgctccatg atggcagcag
     4561 aggggagcga ctggttctgg tggtactaca gccacaacgt gtcggaccag gacgcgatgt
     4621 tcgacgagct atatcgcaac tttctggctg gggtgtacag ggcgttggac cgaccggtgc
     4681 ccgactggat ctcgatcccg atcatcacgc aggtcccgaa gcttaccagc agaaagcctc
     4741 ggggctacat ccgtccgcag ctgagcaaaa ccgatctttc tcctgctccc tgggatctgg
     4801 ccgggtacct ggagcctaaa ggctcaaccg gagccatgca gcagggcagc ggactgctga
     4861 aacggctata ctttggatac gatgacagca agctctactt gaggctggat tgccattcca
     4921 gtttggcagg gtacatcgtg gagctgtata tgtcgcacct ggagggcatc tcctctgtgg
     4981 aggggggcga accgtctcaa ggtgcggaga tacgtgcgtc tgccgcggct gcatctcgag
     5041 acctgtcgat agatgcagcc gcgcgttccg ccgggctgag tctctggcag gagggttcgg
     5101 gctggctggg aatagatggg aagcttctgc tgatggttac ggagaatgtt gtggagcttg
     5161 cggtccctct tgagcagttg ggtgtcggtc tcggggctcg ccttgggctg tctgtggttc
     5221 tgttccggga gggactggtg gttcagaggt tgccggaaga gggcgaggta gtggttgacc
     5281 tggcgctggt cgagttagaa tagtctttta gattgtgtct ttggaactag ccgcgtggaa
     5341 cactgagtga cgagggcccc tgcgaggagg ccggcgtgcc aagctgatcc gcggtttcgt
     5401 gagaggtgga aacttgctaa gcctgcagtt catccgagat cacgccgatg tggttaggga
     5461 gtcgctccgg aagcggcacg aatcggcgcc tgttgaagag gttctagagc tggacgtacg
     5521 gcgccggcag ctgctgcagc agttggagtc gatgcgagcc gagcagaacc gagcctcggc
     5581 agaggttgcg aaggcaaagg acaagtccgc agtggccgat cggatcgcgg agctccggca
     5641 gctctctcaa cgcatcaaag agatggagcc gaccatcaag gagatcgagg agcggcttgg
     5701 tcagttgctc ctgctgatcc ccaacattcc ggatgattcg gtcccggagg gcaaggatga
     5761 ctccgagaac gtcgtgatca gaacttgggg agagcctcgc aagttcgatt tttccccgct
     5821 gcctcactgg gagattggcg agcggctggg gatcatggat ttcgagcggg cggccaagat
     5881 ctccggtgcg cgctttgggg ttctaaaggg gctgggcgcg aagttggaga gggcgctgat
     5941 ctcctttatg cttgatctgc acaccacgga gcacggctac actgaggtcc ttcctcctta
     6001 tctggtgcgc cgcgagtgca tggtcggaac aggtcagctg ccgaagttcg aggaagacgc
     6061 ctacaggact gagccggacg atcttttcct ggtgcccacg gccgaagtgc ctgtaaccaa
     6121 tctgcatcga gatgagatcc tggacgctgc ggcgctcccc atctactatg ccgcctacac
     6181 ggcctgcttc cgccgagagg ccggctcggc cgggcgtgat acgagggggc tcatccgggt
     6241 tcaccagttc gacaaggtcg aactcgtgaa attcgtcgcg ccggagacct cctacgatga
     6301 gttggagaag ctggtcggcg acgcagagga tgtgctgaag cggctggggc tgccctacca
     6361 cgtagcgatg atgtgcaccg gcgatgtggg cttcacggcc gcgaagaagt acgaccccga
     6421 agtgtggatg cctgggcaga acaagtacgt ggagatctct tcctgcagct gtttcacgga
     6481 tttccaggca cgacgagcca acatccgcta caggccggag gcgggtggca agaccgagtt
     6541 cgtacatacc ctgaacgggt cgggtctggc catcggtcgc agcatggctg ccattctgga
     6601 gaactatcag caggcggacg ggtcggtgac gataccggag gcattgcggc catacatggg
     6661 tggcctcgag gcgctggttc cgcccgcacg agcttgactc tcggataccc agtctgacag
     6721 aagagcaggt gggagtaggg ggggaaggaa ggagtgacct ctctggcata attgcgggga
     6781 atccaatcct gcaacgaccg ccaaagaggc cgctccatgg gcccttacct tctgcgactg
     6841 accatcatca aggaggtcgc ccccacctgg ctcaagactc aactcaccaa cctggtgctt
     6901 ctggcttcgg ccctgttcca gtagcgcagc cccgtcctga ctgaactagc ccgggcctac
     6961 ccgatccccg ctgtccgtaa ggtcgctcgt cccagcctcg gccttctgca tcgcgacaga
     7021 cggctcggaa gcttctgtgc cgaccctcgg ctggacgatc ctgccctcat gctgatgctg
     7081 actcggctca gctactccgt ctccgcagtc cggggatgct tctacccgtt ctgctggatc
     7141 tgacctactt agagccctat gctgtcctgt ccgcttactg acttcttgac agtctcgaac
     7201 cctctggcac atgggctgtg gcacaggcga tgcccagagc caagcatgcc tgcacctccg
     7261 gcctccgagt gcgtcaagct aagctttagc acgtcacggg cggctcgcgg acacgatgag
     7321 gaggactgac cagcgcgtgg tggacgaggg catggagggt gagaagaatg gcgagagctg
     7381 aagcccgggt atcaggttcc gcatcgggca acaaggagac agccggcgat tcgtcggcta
     7441 cccgcgtgct tgccgggcaa gaccggatca gggagtggca ggaagacgtc tatcgcacac
     7501 tccaccagca cccggaactc tctaatcagg agttgaacac agcggcaacg gccgccggcg
     7561 cactgcgcga ggccgggtat gaggtgcatg acaaaatcgg gacgaccggt gtcgttggga
     7621 ttctgcggaa cggggacggt cccaccgtgc tcatgcgcgc cgacatggac gcgctgccga
     7681 tgaaggagga gacgggcctt ccgtacgcca gcaccgacca ccagaccgac ggctcgggcc
     7741 aggtcgtgcc ggtggcgcac tcttgcggcc acgacgtgca cgttgcgagc ctgatgggcg
     7801 ccgcccgcct gctcgccgcg aaccgatcgg cgtggcacgg caccttcgtc cccctcttcc
     7861 agccggccga ggaggttggt actggtgcag acgaaatggt caaaggcggc ctcgccacac
     7921 tgatccccaa gcccgacgtc gccctggccc agcacgtcct ggcctaccca gccggcacgg
     7981 tcggtaccct ctccggcccg tttctttcga cggctgccag catgcgcgtc acggtccatg
     8041 ggcgcggcag ccacgggtcg atgccccaac tctccatcga ccccgtcgtg ctggcctcga
     8101 tgatcgtggt gcgcctgcag gcgatcgttg cgcgggaagt ggccccggac gatttcgctg
     8161 tactgactgt cggccaggtc gccgccggga cgaagagcaa catcatcccc gatcacgctg
     8221 tcatcgagct caacatccgc gcctacagtg atagcacgcg cactttgctg atcgacgcgg
     8281 tcaagcgcgt cgtgcagggc gagtgcatgg cttccggctc gccgaaagat cccgagttcg
     8341 agctctacga cgagtatccg ctgacctcca acaatcccga ggtgacggcg aaggttgcgg
     8401 cggccttcaa gggatacttt ggcaaccggg cgttcacagc cccccgtcag tcggccagtg
     8461 aggacttcag ccggattccg gctgcatttg gggttccata cacctactgg gcgctgggtg
     8521 gcatcgacga cacgaagtgg cacaaggcgg aggcggcggg gcggatcgcg agcgacattc
     8581 ctgccaatca ctcgccgaag tttgccccgg tcatcgagcc taccttgaag acgggaaccg
     8641 aggccatcgt ggtcgccgcc ctggcctggc tgtcgtcccc tgccggagtt ccaagggagc
     8701 ggccatgagg cggtgacggg atggggccgg ggtgcgcagt tgcacactga caacgggagg
     8761 gtgggagtga cgaccttgac gacggatacg aaggtcatgc tccctgggaa cgatcggctt
     8821 ctcttcggga tgattctcgg cgtgctggcc ttctggctgt ttgctcagac gaccctgaac
     8881 atcgcgccaa ccatggcggc ggatctgggg atcgcgatga acgtgatgaa catcgctgtg
     8941 tccatcacgg ccctgttctc aggcatcttc atcgtcgtca tgggcgggct cgccgatcga
     9001 gtcggacggg tgaaggtcgt gcagtggggc ttcgtcctca gcatcgtcgg ctcgctgctg
     9061 gtcggtgtga cgccgtccgg ggcgttggca tccaccatcc tggtgctggg ccgcatctgc
     9121 caggggcttt cgggggcctg catcatgccg gcgagtctgg cactggtaaa agcctattgg
     9181 gaaggggccg ggcgccagcg cgcggtcagc ttgtggtcca tcgggtcgtg gggcggctcg
     9241 ggctttgccc cgctgtttgg gggcttgatg gcggaaaacg tcggctggcg gtggatcttc
     9301 ttctcgtccg cagcggtatc ggtgatcggc atgctgatgg tgcgcggcac cccggagagc
     9361 agggccgaag ccaaagggga ctaccggttc gacaccgtcg gcgtcttgac cttcatgatc
     9421 gcaatggtcg ctctgcaggt cttcgtgacg cagggggcga agctgggctg gacgagcgcc
     9481 gcagcactgg gcctgttggg gatgagcctc ctgttcggca tcattttctt ccgcgcggag
     9541 tccggcaatg ccaacgcgtt cgtggacttc cggctcttca ggaatcggat ctatacaggg
     9601 tcgacactct cgaaccttct gctcaacggt gtcgcgggca tccttatcgt ggccatgacg
     9661 ttggtgcaga tcggaggcgg catgagcgcg cagggggccg gcctgctgac actggggtat
     9721 gctatcgcca tcgtggcgtt catccgcgtt ggtgagaagc tgttgcagcg ctttggcccg
     9781 cgcaagccca tgatttgggg atcgctgatc gtgggagtgt cgatcctgct gctcatgccg
     9841 acctacgtcc tgctcggaac ctacaagatc ctggcgatta ttgccttcac actcttcggc
     9901 atcgggctgg gattctacgc cacgccgtcc accgacgcgg cgctcgcgag tctgccggat
     9961 gacgaggctg ggtctggatc gggcatatac aagatggctt catcccttgg gggatccttc
    10021 ggcgttgcca tttcggccac gatcttcacg gcgctcagcg ccgataacgc gccggtgaac
    10081 tggatttcgg gcgtgattac ctttgtcggc cggcaagata acctcgcgct gcgcgaagcg
    10141 gcgttcttcg ccctttgcgc aaatctcctg atggtcgcgg cggccatcat ctcgatcgtg
    10201 ctcaccgtgc caaagggcag gatgagcgag gaaacgcgca aatagcacgc gagcagatgc
    10261 aggaatgccg atggaaacac tcgttctggc actcgatctc gtcgggacct tcgtcttcgc
    10321 gctgagcggc gccatgacga gcgtccggca tcgcctcgac ctgttcgggg tcttcgtgct
    10381 gtccttcgcc gccggcaacg ccggtgggat cacccgcgac ctgctgatcg gggcggtgcc
    10441 cccggccgcg atcgacgatt ggcgctatat cgtggtgtct ctcctggctg gcctggcgac
    10501 gtttcggtgg catccgacgg tccggcggct acagagtctg gtgctggtct tcgacgcggc
    10561 cgggctggcg ctgttcgcgg tctccggcgc gctcaaagcg ctcgccttcc atctgaaccc
    10621 gctcgcggcg gtgttgctcg gggttgtcac cggcaccggc gggggtgcga tgcgcgacgt
    10681 gctcgtggcg gaaatcccca ccgtgctgcg cgccgagctg tacgcggttg ccgcgctggt
    10741 cggcgcgacc gtcgttgttg gtggccagct gctgcaattt ccgtccaccc cgctggcgat
    10801 cgttggtgcg gtcctctgct tcgggctgcg cctcctagca atccggcgtg gctgggggct
    10861 tccggtcgcg cggctgcccg agcaaccgac cgatcgatcg tccctcattc cgtgcggtcc
    10921 tccgt
//
LOCUS       DATALS010000195         4248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_99592,
            whole genome shotgun sequence.
ACCESSION   DATALS010000195 DATALS010000000
VERSION     DATALS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99592"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VHS06_04330"
     CDS             <1..512
                     /locus_tag="VHS06_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEX2987379.1"
                     /translation="QMQNQDPLNPMDDTAFMTQLVQFSTLESMQHMDEHISTLLSVEQ
                     LSQAESMVGKQVTGTVTTDGTTETIEGVVDSVKMVDGSPVLVMGDKSLKLSDVTKVVP
                     KQSSSSDIALASNMIGMQVEAKVGTDATSVSGTVDAVKVVDGEPVLEIGNQSVKLSEI
                     VKVKEGTTN"
     gene            515..895
                     /locus_tag="VHS06_04335"
     CDS             515..895
                     /locus_tag="VHS06_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HEX2987380.1"
                     /translation="MDVQRVQPSDSEGRPQQRLSGAGGGGSGVSFDQVLRAKAAHDQA
                     LRFSSHAQQRMESRGVNLDGEAMARLQRAVDAADQKGAKDALVLMDDLALLVSISNRT
                     VITAVDQSRRKNGVFTNIDSVVMA"
     gene            1043..1924
                     /locus_tag="VHS06_04340"
     CDS             1043..1924
                     /locus_tag="VHS06_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX2987381.1"
                     /translation="MTSGISGMRTHQSAMDVIGTNIANVNTVGYKAGRMSFKEAIYQS
                     YTKGTAPATDGTVGGVNPVQVGLGVTMGGIDNIMTQGTSTSTGKGTDLMIQGEGFFML
                     SKSTASTANGTPDCYTRDGSFSLDADNYLVDPTTGAYVLAADTTSATTSGSKIQIPST
                     WKSFSIGADGTITGVSATGELQEVGAAGGAAGGTAVAVGSGLKVGIAKFPNPAGLSKI
                     GGNYYEAASNAGTMAGGAPGASGNGSVVSGSLEASNVDLAEQFSRMIMAQRGFQANSR
                     IITTSDSMLEELVNLKR"
     gene            2000..4126
                     /locus_tag="VHS06_04345"
     CDS             2000..4126
                     /locus_tag="VHS06_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX2987382.1"
                     /translation="MSEGLSQEKDYYAVLGVPPTADRRTISNAYRSKVKAIHPDSSSS
                     VSDPAAGLHIAELVEAWRVLGDDDKRAEYDLRRAARPSRRAPRSAPPNPTPRPPVLEV
                     FPAVLDFGTVRRGEIVSEGIRIENHGGTATLLERGGTLPEWLSVSIPDVQRKGDPLRF
                     PMELTVRVDSGRLSPHVRHTVEFTLRLASLADGLRSGEVRVSIVVVVGVEEVPHPAVS
                     PGSWLVERAECLGPPRSLLLHLFVGNGGGGRLSVSVKSSPWVRVSSRSFGPIQNESAP
                     VHVAVEVDCVAWKESGWRDGRVALVTPQGDAREAVIISNRTLGGGRLGPFETLWLKWF
                     PLFLLLVAAVPWSVLLGVLPHQTVWFAVVAILLQPMALGPLRRMEMVARGPRVLADGW
                     SLGAPIGGTRFWSSIICWAGIGGALGVGVGTGVFPFLSPGHIPVLGLVAGAICGLSLA
                     SRSGPLARVTSGPELAVSLVEGVGWAAIAVAWCLLCGLGFQAALGGILRLAPIWGGCF
                     GWSVGCLLSIARNGAVPQTWRGRAEEIACAGAPMVVAGLGFAAALALALDLLAVTAKV
                     APVAGYVQGKSSALLALPGSSLTTALVAAVSCIGLVGGYELGLLTLLPAVPLPRPLGA
                     SARKAGTVAKWCAELLLEMAVSHAGLNGSGHEVESKSGPVSTMGIVTRIHWTSLLVTG
                     ITLMLGLYTVGNLLIALCAGVGIALG"
BASE COUNT          788 a         1164 c         1476 g          820 t
ORIGIN      
        1 ctcagatgca gaaccaggat cccctgaacc cgatggacga caccgcgttc atgactcagt
       61 tggtccagtt ctccaccctg gagagcatgc agcacatgga cgagcacata tccacgctgc
      121 tctccgtcga gcaattgagc caagcagagt cgatggtcgg caagcaggtc accggaaccg
      181 ttacgaccga tggtaccact gagaccatcg aaggcgtggt ggactccgtg aaaatggtgg
      241 acggcagccc cgtgctggta atgggcgata agtcgctgaa actgagcgat gtgacgaagg
      301 tcgttccgaa gcagagcagt agcagcgata tcgcccttgc gagcaacatg atcggcatgc
      361 aagtggaggc caaagtaggg accgacgcca cctcagtctc gggtaccgtg gacgccgtga
      421 aggtggtcga tggtgagccg gttctggaga ttgggaacca gagcgtgaag ttgagcgaga
      481 ttgtgaaggt gaaggagggg acgacgaact agaggtggat gttcaacggg tccagccctc
      541 tgacagcgag ggcagacccc agcagcgact gagtggtgct ggcggtggcg gatccggggt
      601 cagcttcgat caggtactgc gggcaaaggc tgcccacgat caggcattgc gcttctcatc
      661 gcacgctcaa cagaggatgg agagcagggg agtgaacctg gatggagagg cgatggccag
      721 actgcagcgg gcagtcgacg cggcggacca gaaaggggcc aaggatgccc tggtgctgat
      781 ggacgatctg gcactcctgg tgagcatcag caacaggacc gtcattacgg cagtagacca
      841 aagcagacgc aagaatgggg tcttcaccaa catcgacagc gtggtaatgg cgtaagtgag
      901 aaaggcgggt tgccggcagg caagaaaggt ctggacccag cagggaggac cgctcgccgc
      961 cgaacgagag aagtggcgag aagccggcga ctgcacgggg ctacaccccc cccgagaaag
     1021 gaaaagtgca gtgcttcgtt ctatgacttc tggtatctcc ggcatgcgaa cccatcagag
     1081 tgcgatggac gtgattggga ccaacatcgc caacgtgaat actgttgggt acaaggccgg
     1141 acgcatgagc ttcaaggagg cgatctacca gtcctacacc aagggcaccg ctccggccac
     1201 cgacggcacc gtcggtggag tgaacccggt ccaggttggg ctgggcgtga ccatgggcgg
     1261 tatcgacaac atcatgactc agggcacctc gacgagcacc ggcaagggca ccgacctcat
     1321 gatccagggt gaaggcttct tcatgctgag caagagcacg gccagcactg ccaacgggac
     1381 accggactgt tacacgagag atggttcgtt ctcgctggac gccgacaact accttgtgga
     1441 cccgactacc ggcgcgtatg tcctcgccgc agatacgacg tcggcaacga cgtcgggcag
     1501 caagatccag atcccttcta cctggaagtc cttcagtatc ggtgccgacg gtacgatcac
     1561 tggtgttagc gcgaccggcg agctccagga agtgggggct gccggcggcg cggcgggcgg
     1621 tacggcggtt gccgttggca gcggtctgaa ggtcggtatt gccaagttcc cgaacccggc
     1681 cggcctgagc aagatcggag gcaactacta cgaggccgcg tccaacgccg gcaccatggc
     1741 cggcggggca cccggcgcca gcgggaacgg ttccgtggtc tcaggtagtc tggaggcgtc
     1801 caacgtggac cttgccgagc agttcagccg aatgatcatg gcacagagag gcttccaggc
     1861 caactctcgt atcatcacta cctccgactc gatgctggaa gagttggtga acctgaagcg
     1921 gtagctgatt gagacgatag gatgatgacg ggtcacccgg cattagagtg ctgggtgact
     1981 ctgttttagg gggatccggt tgtcggaggg actctctcag gagaaggact actacgcggt
     2041 gttgggggtg cccccgacgg ccgacaggag gaccatatcc aacgcttatc gaagcaaggt
     2101 caaggcaatt cacccggatt cctctagcag cgtctccgat cccgcggctg ggctgcacat
     2161 cgccgagctt gtcgaggcat ggcgggtact gggcgacgat gacaagcgcg cagagtacga
     2221 cctgcgccgg gccgctagac cgtcgcgacg cgctccacgt tcggctcccc caaatccgac
     2281 tcccaggcct cctgttctgg aggtcttccc cgcggtcctg gatttcggca ccgtacgacg
     2341 tggtgagatc gtctcagagg gcataagaat agagaaccac ggcggcacgg ccacgctcct
     2401 ggagcgaggt gggactctcc ccgagtggtt gtctgtgtcc attcccgacg ttcagcgaaa
     2461 gggggatccg ctacgcttcc cgatggagtt gaccgtgagg gtggactcgg ggcgcctgtc
     2521 cccccacgtc aggcacactg tcgagttcac gcttcgcctc gccagtcttg cggacggcct
     2581 gcggagcggc gaggtgaggg tgtcgatcgt agtggttgtg ggcgtcgagg aggttcccca
     2641 ccccgcggtc agtccgggaa gttggctggt cgagcgggcc gagtgtctcg gcccgccgag
     2701 gtccctactg ttgcacctgt tcgtgggtaa cggcgggggc ggaaggcttt ccgtgtcggt
     2761 gaagagttcc ccatgggttc gtgtctctag caggtctttt ggccccatcc agaatgagtc
     2821 cgcgccggtc cacgtggccg tggaggtgga ttgcgtcgcc tggaaggaat caggctggcg
     2881 tgatggtcgt gtggcgttgg ttaccccaca gggcgatgcc agggaggcag tgatcatcag
     2941 caacagaaca ctgggcggtg ggcgattggg gccgttcgag accctgtggc tgaaatggtt
     3001 ccctctcttt ctgctgctgg tcgccgccgt gccatggtcg gtgttgctgg gtgtgttgcc
     3061 tcatcagacc gtatggtttg ccgttgtggc aatcctgctt cagcccatgg cgctgggtcc
     3121 attgcgaagg atggagatgg ttgctcgtgg ccctagggtg ctggcggatg ggtggagtct
     3181 gggcgcgcca atcgggggaa cgcggttctg gagcagcatc atctgttggg caggcattgg
     3241 gggggcgctg ggcgttgggg ttggcaccgg cgtctttccg ttcctctcgc caggtcacat
     3301 tcctgtgctt ggactagttg caggtgcgat ctgcgggctg agcctggcat ccagatcagg
     3361 ccccctggct cgggtaacgt ctgggcctga actggcggtg tctctagtgg aaggtgtggg
     3421 ctgggcggcc attgcagtgg cctggtgcct cttgtgcgga ctcggcttcc aggcggcgct
     3481 tgggggcata ctccggcttg cgcccatctg ggggggatgc ttcgggtggt ctgtgggctg
     3541 tctcctctcg atcgcccgca acggcgccgt ccctcagacg tggagagggc gggcagagga
     3601 gatcgcttgc gccggcgcac cgatggttgt tgcaggttta gggtttgccg ctgctcttgc
     3661 gctcgcgctt gatctgcttg ccgtgacggc gaaagtcgcg ccggtcgcgg gatatgtgca
     3721 ggggaagtcg tctgctctat tggcgctgcc ggggtccagc ctcacaacgg ccctggtagc
     3781 ggccgtatcc tgtatcgggt tggtgggcgg gtatgagctg ggcctactca cccttcttcc
     3841 ggcggtcccg cttccgcgtc ccctgggcgc gagcgcgagg aaagccggta ctgtggccaa
     3901 gtggtgcgcg gaattgctct tggagatggc agtgagccat gccggactga acggaagcgg
     3961 tcacgaggtg gagtcgaaga gtgggccggt gagcactatg gggatcgtca cgaggatcca
     4021 ttggacgagc cttctggtta caggaatcac actaatgctc ggcctgtaca cagtgggaaa
     4081 cttgctgatc gccctgtgcg ccggtgtcgg tatcgccttg ggctaagggc acttcaccca
     4141 gttgcgcagg gggccgaagg aggagtcgtg atttctagga gcgcagcgtc acgctcgacc
     4201 gatctattat gagtagtgtt tgcttttcga tctgataggg cgggtcgc
//
LOCUS       DATALS010000196         5460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_279723, whole genome shotgun sequence.
ACCESSION   DATALS010000196 DATALS010000000
VERSION     DATALS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279723"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VHS06_04350"
     CDS             <1..1022
                     /locus_tag="VHS06_04350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2987383.1"
                     /translation="IHWLKAVTPTKARVQVTTWIPAFAGMTVRNVVLLSSRVSCYVAA
                     GQIGQYSMIRSAYVGRWRWSDKTAMLLAFGGVSVTLIEGQVNAMFLLAFSLALLALKR
                     DRLITGGALLGVLWLKPQYAAPFALLLLVKCRWRELAGMVGVGAGIGALSFAMVGPEG
                     ILEYLKELQRIGAFRPPADALVSPQAMVNWRAMLLNLWPDVPDGAGSVLVLLLGGATI
                     IAALLNCRGEWAPGSPLFARQMLVMTLATILASPHSHFHGAVLLLAPLAALLARSPAE
                     AAPVRGWRMLLVGGLVLSIVAWPVGRGRWVMAPYYLVCLGMLICCRPPTLSMVRGDRM
                     ARSGD"
     gene            1088..2035
                     /locus_tag="VHS06_04355"
     CDS             1088..2035
                     /locus_tag="VHS06_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987384.1"
                     /translation="MHRCLTAVVTFVMAAIALTPSALSLPQHIDEVQYAYISDYFGGK
                     ISRLDFTPVSGSDEWLDPGWSPSSFWALTQPMGPRYLYAAALGITQAPPPARPWIWGD
                     FVSYNPLTQLTDGTRNTIRFTAVLLSALGLALIAYRLGWSAVVAIVVFLLIPHVREDL
                     ARGWAEGPLLFGLGLAAAAWGTRWFAPACGFAVAVKLNALPLLLLAFYGGFGRSRYRH
                     ILGVTVALVVWTILMPPAWYSYGPAFLVLMILMRWVEFGSQSRFADPEVWGGLFGHYF
                     PTRYLWPVELVVLLTAMYLVARYRHRLVRLIPLPRRRTA"
     gene            2433..2801
                     /locus_tag="VHS06_04360"
     CDS             2433..2801
                     /locus_tag="VHS06_04360"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEX2987385.1"
                     /translation="MSVDKMMMADNPFANVLSLIADGLRRNRVALLIALALNVGDAIS
                     THIGLTGGLQEGNAIPALLLANGGELAWVGSKFTLVAVVVLLVCLLGRRYPKLWHTFT
                     VTNFVLLAAVLSNSAQLLTQ"
     gene            3659..4573
                     /locus_tag="VHS06_04365"
     CDS             3659..4573
                     /locus_tag="VHS06_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX2987386.1"
                     /translation="MALFFLVCDLLLEGWSSNAFVDALLLIGGFVLVEALRVTAQWHW
                     FWWVLQVPEYPLFPKPFRLFGVLSSPNLLAGQLILILPFAILKFGRARSSVGALVWGC
                     WLLAADVVLFLTSCRGGWVATIVEVAICVGWLACRRECIREENAVGWIVERWRIWVAV
                     ATYVGLFGLLQILSSSASPLPIASHDNFSPQEPAGRIAIWAVAWQDFLSHPLVGSGPG
                     TYPWILVSQTATSSGGVASPHSHNIFLEALAEQGVLGMIALVWGLLAAASTLGPALLR
                     AVRTNRDAGSADSQLVVGVSADLASLQR"
     gene            complement(4667..>5460)
                     /locus_tag="VHS06_04370"
     CDS             complement(4667..>5460)
                     /locus_tag="VHS06_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987387.1"
                     /translation="SLMNASELADRVGFTDEADQLLMGALPRGRGRLLPALLLAERYA
                     DRGRASEAESLFSSALARAPGLVATAAVQGSAAGRDMASRLRPESRSSRNRDLAAHEL
                     PSEEEAAETLRSLSVVAPENADYSVWLDRAVAHMTLGETRQARHSLAVARDLGDETNS
                     AFALASAHYYEAAGQEGMAIKALERAVNIRGAQAAYSLFVYSRPTLPGELLPDLAMLE
                     RTADDLLAYRELARLYANQGRMGDADRVERDAEVLARLLEGDGSD"
BASE COUNT          893 a         1577 c         1678 g         1312 t
ORIGIN      
        1 ggatacactg gctcaaggcc gtcaccccca cgaaagcgag ggtccaggta actacatgga
       61 ttcccgcttt cgcgggaatg acggtacgga acgtggtgct cctctcctca agagtgagct
      121 gttacgtcgc agccggccag atcggacagt acagcatgat ccgctctgct tatgttggca
      181 ggtggaggtg gagcgacaag acggcgatgc tgctggcttt cggcggcgtg tcggtcacgc
      241 tgatcgaggg acaggtgaat gcaatgttcc tgttggcgtt cagcctggct ctgctggcgt
      301 tgaaaagaga caggttgatc acgggcggcg cgttgctcgg ggtactgtgg ctgaaaccac
      361 agtacgctgc gccctttgcg ctcctcttgt tggtcaagtg ccggtggaga gagttggctg
      421 gaatggtggg cgtcggcgca gggatcggcg cgctatcgtt tgcgatggtc ggccctgaag
      481 gcattctgga gtatctcaag gagttgcagc ggataggcgc cttccggcca ccggcggacg
      541 cgctggtcag cccgcaggcg atggtcaact ggcgcgccat gttgctcaat ctatggccgg
      601 atgttccaga tggcgccggc agtgtcctcg tgttgttact gggtggggcg acgatcatcg
      661 cggcgctgct gaactgccgt ggagagtggg cacctgggtc accgctgttc gcgaggcaga
      721 tgctcgtgat gactctggcg acgatattgg cgagcccgca cagccacttt catggggcgg
      781 tcctgctgct ggcgccgttg gcggcgctgt tagctcgttc gccggccgaa gcggcgcccg
      841 tgagaggatg gcgtatgttg cttgtgggag gactcgtgct ttcgatcgtg gcatggcctg
      901 ttggtcgagg tcgctgggtc atggctccgt actatttggt ttgccttggc atgctgattt
      961 gttgtcggcc accgacactg agtatggtac gcggtgatcg catggcgaga tcgggtgatt
     1021 aggttcgatg gaaggctcgc aaaccacggt catctgctag tttcagttcg gctatccgga
     1081 ggttggaatg catcgttgtt tgactgccgt tgtcacgttc gtgatggcgg cgattgctct
     1141 gacgccctct gccctctcgc tgcctcagca tatcgacgag gtgcagtacg cgtacatctc
     1201 cgactacttt ggtggcaaga tcagtcgcct cgacttcact ccggtcagtg gcagcgacga
     1261 atggcttgat ccgggttgga gcccttcctc attctgggcg ctcacccagc cgatgggacc
     1321 gcgctacctc tacgccgcgg cgctgggcat cacgcaggcg ccgccgccag ctcggccgtg
     1381 gatatggggc gacttcgttt cgtacaaccc tctgacgcag ctcaccgacg gaacacgtaa
     1441 cactatccgc ttcacggcag tgctgctatc cgccctcggg ctcgcgctga tcgcatatcg
     1501 cctgggatgg tcggccgtgg tggcgattgt cgtgtttctg ctgatcccgc acgtccgaga
     1561 ggacctcgct cgtggttggg cggaaggtcc gttgctcttc ggcctcggcc tcgccgctgc
     1621 agcgtggggg acgcgctggt tcgcgcccgc gtgtggtttc gcggtagcgg tgaagctaaa
     1681 cgccctgccc ctgttgttgc tcgccttcta tggtggcttc ggtcgctcgc ggtatcggca
     1741 catccttggc gtcaccgtgg cgttggtcgt gtggactatc ctgatgccgc ccgcctggta
     1801 ttcgtacggt cccgcgttcc tcgtgttgat gatcttgatg cgatgggtcg aattcggcag
     1861 ccagagtcgc tttgccgatc ccgaggtgtg gggtggcctc tttggccact acttccccac
     1921 ccgctatctc tggcccgtgg agttggtggt tctcctgact gcgatgtatc tggtcgctcg
     1981 ctatcggcat cgccttgtcc ggttgatacc actgcctcga agacgtaccg cctgaactgc
     2041 cgccatgacg ctgcgtggac cacggtcgac ctgactccct cggtttctcc cccactccga
     2101 cggggatggg cctcttccct agcggaggga tatcgcaagc ttcagccgag cgatggtgcg
     2161 ataccaaggg ctacaattct tctcccacgg gcctcgaccc ccagagttcc gactctgggg
     2221 ggtcgagata tcaacaacca ctaaagttct cgctacatct gccgatattt ctatgtagtt
     2281 ggagctatgc cgccagtccc tttgccggat atgcgaagtg gaaagaggtg cggcaggctg
     2341 tggcaacgat cttgcaccgc cagtttactg aagggttcac cgtacgctgg acctgtggtc
     2401 ttcctgtcgg tgcagaactg ggagtcgccc aaatgagcgt cgacaagatg atgatggctg
     2461 acaacccttt tgcgaatgtg ctgtccctga ttgctgatgg cctgcgccgc aaccgggtcg
     2521 ccttgcttat cgcgcttgct ctgaacgtgg gagacgccat ctcgacgcat atcgggctta
     2581 ccggtggttt gcaggagggg aacgcgatac ccgcgttgct gttggcgaac ggcggcgaac
     2641 tggcgtgggt tggctccaag ttcaccctcg tggcggtggt ggttcttctg gtgtgcttgc
     2701 tgggaagacg ttatcccaag ctctggcaca cctttaccgt gaccaacttc gtcctccttg
     2761 ctgcggttct ctcgaactcg gctcagctcc tcactcagta acggcacccc ttcgcctctc
     2821 gatcgactgt gagcctattt tggactctaa acagatggcc gaagatagcc tgcccttcgt
     2881 cacccccgcg gaagcggggg tccaggctat agtacgtgtc aatgcatgtc aatcgctctg
     2941 gattcccgct ttcgcgggaa tgacgatctg gttgttcaca cgatctcagg ctaggtgctc
     3001 aggaaggtga tgccggttag aacccgtctc catcgtgccc cctaaatact cagtcgacgg
     3061 caactgattt cggttgctgg ccgaattatg atgtactaag ctgtgcattg ccatatatca
     3121 atgcctcttg gcgcgacgtt cccattgtcg gcggaatggc gactaacgat gctcagacgc
     3181 ggttggcttc tgcatccgag cgatgacgta gccgccgtga tgccatgaac cctggtgttg
     3241 ccaatacctg ggcggtatcg ttgcctgtca catgcttgcc caagttttgg cacctgacta
     3301 cgagatgggc tcgcggacgg ttccccgaag tatgcgaagg aggaagcctg gcatggcgtt
     3361 gtttcgcagg tgggagcccg ctcacgtgct acggaccgct ttcctgttct cgctcttgtt
     3421 ctcatacacc cgactgatcg ccttcagaga atttccacct cttgtgttcc tcatacaggg
     3481 gggagtggtt ctcttgctct gcggttggtg gatctggaag gtataccggc gacagggaat
     3541 cgctcgttcg cctttggctc tcccagccgt agcgttggtg atggcctcag ttgcgaccac
     3601 gccattctct gtcgaccctc ggatcagtta cgcctagtct ctcggtctgt tcgcacaggt
     3661 ggcactgttc ttcctcgttt gtgacctcct tttggaggga tggagcagta atgcattcgt
     3721 cgatgcattg cttctgattg gaggtttcgt cctcgtcgag gcattgcggg tgactgctca
     3781 gtggcactgg ttctggtggg tgttgcaggt accggagtat cccctgtttc ccaaaccgtt
     3841 ccgattgttc ggagtgctgt ctagtcccaa tttgttggct gggcagttga tcctgattct
     3901 cccattcgcg atcttgaagt ttggccgggc acggtcgtct gtcggggcgt tggtgtgggg
     3961 atgctggtta ctggcagctg atgtcgtcct gttcttgacg agttgtcgag gtggttgggt
     4021 cgccaccatc gtggaggtgg cgatttgcgt tggctggtta gcctgccgaa gagagtgtat
     4081 tcgcgaagag aatgcggttg gctggatagt ggaacgctgg cggatatggg ttgccgttgc
     4141 cacctacgtt ggtctgttcg ggcttctcca gatcctgagc agctcggcct ctccgctgcc
     4201 catagcctct cacgacaact tcagccctca ggagccggca ggccggatcg ccatctgggc
     4261 cgtggcctgg caggactttc tttcccaccc actcgttggc agtggacctg ggacgtaccc
     4321 atggattctc gtttcgcaaa ccgccacatc gagcggtgga gtcgcttctc ctcattccca
     4381 caacatattt cttgaggccc tggctgagca gggtgtgctc gggatgattg ctcttgtgtg
     4441 gggactgctg gctgctgcct cgacccttgg tcctgccttg cttcgagcag tgaggacgaa
     4501 cagggacgca ggcagtgctg attcacaatt ggtggtcggg gtgagcgccg accttgcttc
     4561 actccagcgt tgaaccatgt caactgtacg taggacactg ctgagattgc ggcttcgatc
     4621 gctcgcggga ccgcccgtat cgctagaaag ccacacgttg gaacgctcag tcgctcccgt
     4681 cgccttcgag cagtcgggcc aacacctcag catcccgctc cacgcggtcg gcatcgccca
     4741 tccgaccttg attggcatac aatctcgcca actcgcggta cgcgaggaga tcgtctgcag
     4801 tccgttccag catagcaagg tctggcagta gttcaccagg taaagtgggg cgcgaataca
     4861 cgaagagact gtacgctgcc tgagcccccc ttatgttaac tgctcgctct agcgccttga
     4921 tagccatccc ttcctgtcca gcagcctcgt agtagtgggc actggcgaga gcgaacgcgg
     4981 agttggtctc atccccgagg tcccgggcaa cagccagcga atggcgggcc tgcctcgttt
     5041 ctcccaatgt catgtgcgcc acggctcgat ccagccaaac gctgtagtcg gcattctcgg
     5101 gagcgactac gctcagcgat cggagggttt cggctgcttc ctcctccgaa ggtaattcgt
     5161 gggcagctaa atcgcgattc ctgctcgacc tgctctcggg acgaagccgg gaagccatgt
     5221 cccttccagc ggcactgccc tgaacggcag cagtcgcgac taagccaggg gcccgagcaa
     5281 gtgcactgga gaacaacgac tctgcttcgg atgctcttcc tcggtcagca taccgctcag
     5341 ccagcaacaa ggctggaagt aatctccccc tgccgcgagg aagcgccccc atgagaagtt
     5401 ggtccgcttc gtccgtgaaa ccgacacgat cggccagctc agaggcgttc atcagacttt
//
LOCUS       DATALS010000197         4747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_202017, whole genome shotgun sequence.
ACCESSION   DATALS010000197 DATALS010000000
VERSION     DATALS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202017"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VHS06_04375"
     CDS             <1..345
                     /locus_tag="VHS06_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX2987388.1"
                     /translation="SVLLESSTQLIANHQSYADPAKRTEIEEIQTLLTGVLTARGKVL
                     IKLNVSEENLQAVVDVIPGMKSPTVSRLFGSGYYAVEAVAVKSEVNLLIPRLKAAGAE
                     DILELPISKIVL"
     gene            570..986
                     /locus_tag="VHS06_04380"
     CDS             570..986
                     /locus_tag="VHS06_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX2987389.1"
                     /translation="MADQRLLDILNRSIARELQVSIQYMWHHVQAVGLESPAVKDIFR
                     DIAIVEMKHAERFAERLDLLGGVPTTKPDPIMQGGSLQKMIEDDLKAEQEAVDMYSEA
                     VRIATEVNDPVTRLMYEEILDAEEDHRKIFGDLLKR"
     gene            1197..1418
                     /gene="infA"
                     /locus_tag="VHS06_04385"
     CDS             1197..1418
                     /gene="infA"
                     /locus_tag="VHS06_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX2987390.1"
                     /translation="MPKDQAIEIEGTVIEALPNATFRVQMEGGHEVLAHASGKMRKFF
                     IRILPGDRVKVELSPYDLTRGRIVYRYTK"
     gene            complement(1450..2517)
                     /locus_tag="VHS06_04390"
     CDS             complement(1450..2517)
                     /locus_tag="VHS06_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2987391.1"
                     /translation="MEDGYARLAAALDALPNGFPRTPGGKELRILQKIFSPEDAELVS
                     YLTGQMELPTSIAARAGIAPEQVNARLKALAKRGMVWLDMDRQARSWRFRLAPFVVGI
                     YEASLELMDHELAHLVEDYMMEGGAEGIMRPEPALQRVLPARHALKTEWVLPYEDVVK
                     ILEGARSFSVRECICRVQQEQMGERKCDFPTHNCLSFSGFGRPPSPDNISREEALDLL
                     DRCEELGLVHTVSNVVKDVYYVCNCCGCCCGILRGVTQRGIENSVARASYLAEVDAQM
                     CTGCGACTHRCQVGAVAIVDEVATISPERCIGCGLCVTGCTAGAATLQRRPDWEAIRP
                     PETFAEWEHQRLHHRGERPLL"
     gene            complement(2610..2963)
                     /gene="yedF"
                     /locus_tag="VHS06_04395"
     CDS             complement(2610..2963)
                     /gene="yedF"
                     /locus_tag="VHS06_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HEX2987392.1"
                     /translation="MEARMDGRTVLVLDAATLGRGDDELGAQLIVNFLRTMAFRDEVP
                     ETVVCYNGGVKLAEQGSPAVPMLEALSQKGAEILLCGTCVNYYQLGERLALGRVSNMQ
                     EIVEVISTAAKAIYV"
     gene            complement(3003..4352)
                     /gene="murA"
                     /locus_tag="VHS06_04400"
     CDS             complement(3003..4352)
                     /gene="murA"
                     /locus_tag="VHS06_04400"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX2987393.1"
                     /translation="MERFIIEGGAQLHGTVTPSGNKNSALPILAASLLTDEPLTVRNV
                     PRIRDVEDMLALLVDLGVEITELSRHDLVLQARDVRTTELSREICAQIRASILLAGPM
                     LARAHEIKLPPPGGDVIGRRRVDTHLTALRALGAQVSIGNGFEMRTSGLRGSEIFLDE
                     ASVTATENALMAAATAQGTTVLMNAASEPHVQELARCLNSMGARISGIGANALIIEGV
                     ERLHGADVTIGPDHIEVGSFIGMAAATHSELLIENAAPEHLSMVFIAFERLGVNCKVC
                     GESGTAALEPHERGVDFARRSTAGCNVLVPQDQSLKIESDLHNAVPKIDDGPWPAFPS
                     DLMSIALTVATQCDGTILMHEKMFEGRMFFVDKLLNMGARVVLCDPHRAVVVGPSKLH
                     GERLESPDIRAGMALLLAALSAHGRSIINNIGQIDRGYERIEEKLQRLGARIERVKV"
     gene            complement(4395..>4747)
                     /locus_tag="VHS06_04405"
     CDS             complement(4395..>4747)
                     /locus_tag="VHS06_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987394.1"
                     /translation="AMALDLPAQLRGRTMVRLGNLYKRLRQRQDAVEVWRKLASVGYS
                     YTVVAYVELAKHSEHVSRDYLAAERLTTQALAALELRATRGDPWRVEQERRELEHRLA
                     RLRRKLAKDGRQAG"
BASE COUNT          926 a         1553 c         1381 g          887 t
ORIGIN      
        1 tccgttctgc tggagtcctc cacccaactg atcgccaatc accaatccta cgcggacccc
       61 gccaagcgga cggagatcga ggagatccag acgctgctaa ccggcgtact caccgcgcgg
      121 gggaaggtgc tgatcaaact gaacgtcagc gaggagaacc tccaggcggt ggtggatgtg
      181 atcccaggta tgaaatcccc gacggtctct cggctcttcg gttccggcta ctatgccgtg
      241 gaagccgtcg ccgtgaagtc cgaggtaaac ctgctgatcc cgcgtctcaa ggcggctggg
      301 gcagaggata tcctggaact gccgatctcc aagatcgttc tctagctaga tagactggta
      361 cagaacagca aagacaccag gtgcaagatc ctggtgtctt tgtcatttgt cattcgtcca
      421 catcgtgttt gcgtatcact cctactttac agcttcgtgg catgcttgta gtatgaggtt
      481 gcttctttct tgcaagagat tcgtggaacc gtcaactcca caagaccgag acgagaatcc
      541 cagcgaagag tgtgtaggag gcaaggcaaa tggccgacca gagattactg gacattctca
      601 accgatccat cgccagggaa ctccaggtgt caattcagta catgtggcac cacgtgcagg
      661 cagtcgggct ggaaagccct gcggtgaagg acatcttcag ggatatcgcc atcgtggaga
      721 tgaagcacgc ggagcggttc gccgagcggt tggacctgct gggcggggtg ccaaccacga
      781 agccggaccc cataatgcag ggcggcagcc tgcagaagat gatcgaggat gacctcaagg
      841 cggagcagga agcggtggat atgtacagcg aggcggtccg aatagccaca gaggtcaacg
      901 atccggtaac caggttgatg tacgaggaga tcctggatgc cgaggaggac caccggaaga
      961 tcttcggcga cctactgaaa aggtagtggc ggaaccagcg acggagatcg atgtagattg
     1021 ccgaatggca aaccgaagac ggggcaaagc agcaggggcg acgaagccgt cgcccctgct
     1081 ttcttatcaa ccacactgtt aggaatggct cctgcgtgct aaactagatc gaatgcgtgg
     1141 cttgaggtca gactagagtg ggcggggcac gttgcgcccg tctcgaaagg agcgttatgc
     1201 cgaaggatca agcaatcgag attgagggaa ccgtcatcga ggcattgccg aatgccactt
     1261 tccgagttca gatggaaggt gggcacgagg tgcttgccca cgcctcgggc aagatgcgca
     1321 agttcttcat ccgcatactt cccggcgatc gagtgaaggt tgagctgtcc ccttacgacc
     1381 tgacccgagg ccggatcgta taccgctaca ccaagtaggg gatcgagggc gggggacagc
     1441 gccgtatcgc tatagcagcg gcctttctcc ccggtgatgt agtcgctggt gctcccactc
     1501 agcgaaagtc tcaggggggc gaatcgcctc ccaatcgggc ctgcgctgca gggtagctgc
     1561 accggcggtg caccctgtaa cgcacaaccc gcaaccgatg catcgctcgg ggcttatggt
     1621 cgcgacttcg tccacgatgg caacagcccc tacctggcac cggtgcgtgc aagcgccgca
     1681 tccggtgcac atttgggcat ccacttccgc caggtagctt gcccgggcca cggagttctc
     1741 tatcccacgc tgcgtaacac ctcggagtat cccgcagcag cacccgcaac agttgcaaac
     1801 gtagtagacg tccttcacga cgttgctgac ggtgtggacc aggcccaact cttcgcatcg
     1861 gtccaggagg tccaatgcct cctccctgga gatgttgtcc gggctggggg gacggccgaa
     1921 gcccgagaag gataggcagt tgtgggtggg gaaatcacat tttcgctctc ccatctgctc
     1981 ctgctgcacc ctgcagatgc actcacgaac ggagaagctg cgggcaccct ccaggatctt
     2041 caccacatcc tcgtagggca gaacccactc cgtcttcagg gcatgccggg cggggaggac
     2101 ccgttggaga gccggctcgg ggcgcattat cccctccgcg ccgccctcca tcatgtaatc
     2161 ctcaaccaga tgggccagct cgtggtccat caactccagc gaagcctcgt agatgccgac
     2221 aacgaagggg gcaagcctga agcgccagga gcgggcttgg cgatccatgt ccagccacac
     2281 cataccccgc ttggccagcg ccttcagtcg ggcgttcacc tgctccgggg cgatccctgc
     2341 ccgggccgcg atactggttg gaagctccat ctgtccggtc aggtagctga ccagctcagc
     2401 atcctcgggg gaaaagatct tctggagtat tcgaagctcc ttgccacccg gcgttcgagg
     2461 aaagccgttg ggcagggcgt ccagtgccgc cgcgagcctg gcgtagccgt cttccacagt
     2521 cccgcctcca atgcagttct gggttctggg ttctgagttc tgagacaatt ccactcagca
     2581 cccagaaccc agaactcaga acccagaacc tacacgtaga tcgccttggc cgccgtggag
     2641 ataacctcca cgatctcctg catgttgctc acccgcccca gcgcaagccg ctcgcccagt
     2701 tggtagtagt tgacgcaggt gccgcacagc aggatctccg cccccttctg ggacagcgcc
     2761 tccagcatcg gcaccgccgg cgagccctgc tccgccagct tcacgccgcc gttgtagcac
     2821 accaccgtct cgggaacctc atcccgaaag gccatcgtcc gaagaaagtt cactatcaac
     2881 tgagcgccca actcatcatc cccgcgccca agggtcgcgg cgtcgagcac caggacggtg
     2941 cgcccgtcca ttcttgcttc catctcatca tcccgcacgg ttcggccgct tcgagagcca
     3001 gcctatactt tcacccgctc gatcctggcg ccgagccgct gcaacttctc ctcgatccgc
     3061 tcgtagcctc gatcgatctg cccgatgttg ttgatgatgc ttcgcccgtg ggccgagagg
     3121 gccgccagca gcaacgccat gcccgcccga atgtccggcg actccagccg ctcgccgtgc
     3181 aacttgctgg gcccgaccac cacggcgcga tgcggatcgc acagcaccac ccttgccccc
     3241 atgttcagca gcttgtccac gaagaacatc cgcccctcga acatcttctc gtgcatcagg
     3301 atcgtcccat cgcactgggt ggccaccgtc agggcgatac tcatcaagtc cgaggggaag
     3361 gccggccagg ggccgtcgtc gatcttgggg acagcattgt gcaggtcact ctcgatcttc
     3421 agcgattgat cctgcggcac gagcacgtta catcccgccg tggagcgtcg agcgaaatcc
     3481 acgcccctct catgaggctc cagcgcagcc gtcccgctct caccacacac tttacagttg
     3541 acgccgagcc gctcgaaggc gataaacacc atggacaggt gctccggcgc ggcgttctcg
     3601 atcagcagct cactgtgggt tgcggcagcc atcccgatga agctgcctac ctcgatgtgg
     3661 tcaggcccga tggtgacgtc ggcaccatgc aggcgttcta ccccttcgat tatcagggca
     3721 ttggcgccga tgccgctgat gcgggcaccc atgctgttga gacaacgcgc cagctcctgc
     3781 acgtgaggct cactcgccgc gttcatcagc acagtcgtcc cctgtgccgt cgccgcggcc
     3841 atcagggcgt tctccgtcgc ggtcacgctg gcctcgtcca ggaatatctc gctgccgcgg
     3901 aggccggacg tacgcatctc gaagccattg ccgatgctca cctgggcgcc cagcgcacgc
     3961 agagcagtca ggtgggtatc cacccgccgc cgcccaatta cgtcaccgcc cggcgggggg
     4021 agcttgatct cgtgggcgcg ggccagcatg ggccctgcca gcaggatcga tgcacggatc
     4081 tgagcgcaga tctcgcggct caactccgtc gtgcggacgt cccgcgcctg cagcaccagg
     4141 tcatgcctgc tcaactccgt gatctcgacg ccgagatcca ccagtaaggc cagcatatcc
     4201 tcgacatcgc ggatccgcgg cacgttgcgt acggtgagcg gctcatcggt caacaggctc
     4261 gccgcgagga tcggcagcgc ggagttcttg ttgccgctgg gcgtgaccgt gccgtgaagt
     4321 tgtgctccgc cctctatgat gaacctttcc aatggccgtc tccataattc atgtcaatgg
     4381 tagagcgggt tgtctcaacc cgcctgtcgt ccatccttcg ccagcttgcg ccgcagccga
     4441 gccagccgat gctccagttc tcgccgctcc tgctccaccc gccacggatc tcccctggta
     4501 gcacgcagtt ccagtgcagc cagcgcctgg gtcgtaagcc gctccgccgc aaggtaatcg
     4561 cgcgagacat gctcgctgtg cttggccagc tccacgtagg ccacgacagt gtaactgtat
     4621 cctaccgagg ccagcttacg ccacacctcc acggcatcct gccgctggcg aagccgctta
     4681 tacaggttgc ccagcctcac catcgtccgg ccccttagct gcgccggcag atcgagcgcc
     4741 atggccc
//
LOCUS       DATALS010000198        15364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_279898, whole genome shotgun sequence.
ACCESSION   DATALS010000198 DATALS010000000
VERSION     DATALS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 15364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15364
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279898"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VHS06_04410"
     CDS             complement(<1..335)
                     /locus_tag="VHS06_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015224280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEX2987395.1"
                     /translation="MESPAALAESQNSPAEGAGSGERSRIRIWVVPHTHWDREWYFPF
                     QLFRLFLVRTTDELMDVLEADPSFSSFTFDGQGIALEDYLEIRPEAEARLARLIRRGR
                     IRIGPSYILP"
     gene            791..1216
                     /gene="msrB"
                     /locus_tag="VHS06_04415"
     CDS             791..1216
                     /gene="msrB"
                     /locus_tag="VHS06_04415"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX2987396.1"
                     /translation="MKSRVTKSDREWQETLTPEQYYVTRRKGTEPPYSGKYNRLHDKG
                     VFRCVCCGNELFSSDAKFESRTGWPSFTEPISEGSVTTREDRSLFMERTEVLCARCEA
                     HLGHVFDDGPRPTGLRYCMNSVALDFEAEDQEEGSRGEG"
     gene            complement(1314..1694)
                     /locus_tag="VHS06_04420"
     CDS             complement(1314..1694)
                     /locus_tag="VHS06_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX2987397.1"
                     /translation="MEGQENRGLSAVKAALQAELDGLNQHLREMADTAQRERRAEGNR
                     PGFGKRVGDYTAEVQEATNNRAVVQNMGRAMDELRRALEKVDEGSYGLCDGCGRPIAP
                     ERLEVMPSAVLCIECKRKREQRRR"
     gene            1772..2194
                     /locus_tag="VHS06_04425"
     CDS             1772..2194
                     /locus_tag="VHS06_04425"
                     /inference="COORDINATES: protein motif:HMM:NF033789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HEX2987398.1"
                     /translation="MTDEESTSPLHVVLAPLTRRQAYAMLISVFGEISKEWEMGVNDV
                     FRALSDPTRREILRLLQAGDMTAGEISDRFPLARSTLSGHFNVLKQAGLIVMERQGTR
                     IVYSLDTSAFDEAMATILDLFGVGKQERGGGNAHHVED"
     gene            2172..2849
                     /locus_tag="VHS06_04430"
     CDS             2172..2849
                     /locus_tag="VHS06_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEX2987399.1"
                     /translation="MRITWKTEWPNWVLILGMFVMAGLSWASAPDRIPVHWNITGQVD
                     RYGGKVEGLLAIPLITLSTYILLILTPLIDPGRANYERFAGVYSLIRTLLTVLFAAIY
                     AVVLLWVRGYQVDVSAIIPIAIGAFFIIIGNYMGKIRPNWFVGIRTPWTISSKRSWTK
                     THRLGGWIFILMGLVLILSGVVRTEWAAYLSFAVIIGGVIWTFVYSYLVWRNDPDRIP
                     PSGTLPG"
     gene            2929..4017
                     /locus_tag="VHS06_04435"
     CDS             2929..4017
                     /locus_tag="VHS06_04435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2987400.1"
                     /translation="MIASFSETDNRVNWRQVGAFVALTFGLTWALDAAIALTVGYRSP
                     AFVLLLQLQMLMPAFSAILLGSFFFTDSPIYRRVPLGSPRHFFTFFLVYTIIYMILGW
                     ASIAAPQQSQLFSTLGLALTILGLLFILLLRVRGGRQQFGTAGLAGGNPRYWLLFGVA
                     VLLFYSFQTLLNSLLGLGHPADLSALTGQTGMSDSVLLLIAAVQSVVVGPLIALPIAF
                     GEEYGWRGYLQGELVKLGKMRGVLLVGTIWGLWHAPIIAMGHNYPGHPVEGILLMVAY
                     CIGLGYFLGFAVLKSGSVWLAAFLHGLNNQALGYLSGLINTPNDPVYSFGVGIYGMVP
                     LAIVTLLILRDHVWKESTRAGTVSSQLS"
     gene            complement(4043..5353)
                     /gene="odhB"
                     /locus_tag="VHS06_04440"
     CDS             complement(4043..5353)
                     /gene="odhB"
                     /locus_tag="VHS06_04440"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX2987401.1"
                     /translation="MTAEIVVPVLGESVVEATVGKWLKSVGESVAAGEPVVELETEKV
                     NMQVAAEEPGVLSEIVHGEGDTVHPGDLLARLELGAPSPAPGAPPQEPQAVQPSPPRE
                     PAAPSAAAAPPSPVREVEKAHVSPVAQKLAAELKVDLSAVQGSGPGGRIMKEDVEAVA
                     RQREAAGAPPSPAPAPTPPAPTAPPTATAPAAAVRAVPSPVAGEREEIRQRMSRRRLT
                     IARRLVEAQHTAAMLTTFNEIDMSAVMDLRRRQRESFKAAMGVDLGFMSFFVKAVVGA
                     LKAFPEINAELQGEELVLKHYYDIGIALGDPEGLVVPVLRDADRMSFAEIEKAIALFV
                     QKSKERSLTLEELRGGTFTITNGGVFGSLMSTPILNPPQVGILGMHKISERPVVVNGQ
                     MAIRPMMYVALSYDHRVVDGREAVLFLVKVKELLEEPARMLLEG"
     gene            complement(5372..8254)
                     /locus_tag="VHS06_04445"
     CDS             complement(5372..8254)
                     /locus_tag="VHS06_04445"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX2987402.1"
                     /translation="MDGNVGRGWEEFSGLNAAYMAELFERYSADPASVDPEIRSFFDR
                     WGPPRLPVQAGEVAPTVAAPGVDCDLISAARALSTAIRIYGYRAARLDPLGSEPPGDP
                     ELLAENHGLREGQLSAMPPSVIGGPVAERAGSAKEAIARLRQVYQGTTGYEFEHVYNA
                     EERRWLRNAVESGRYSPPQDLIDARWLLERLTQAETFERFLHTAFPAQTRFSMEGLDM
                     LLPMLDELMGAAAEIGTQSVLLGMAHRGRLNILAHLLGKSYEEILAEFRGSLQRPNVS
                     ATDSSGVGWTGDVKYHLGARRAFRGGREVGMLVTMAPNPSHLEYVDPVVVGMTRANSE
                     RRDQRGPARLDERITLGVLIHGDASFVGEGIVAETLNLSMLPGYHTGGIVHIIANNQL
                     GFTTSSRLGRSTLFASDPAKGFEVPIVHVNADDPEACIAAVRLAHAYVERFHKDVLID
                     LIGYRRWGHNEGDEPTYTQPTMYAVISGHPAVRELWAKELVRRGSITPEEAEGMVRAT
                     MERLQAARRSLPSMDGVTTGDGQQAGMEAGGTRGGLGNQGVAQEVETRVPSETLAELN
                     RQLLELPPGFKLNSKLEPIYNRRRSAFASSAEEGQKGGVDWAQAEQLAFASLTAEGTP
                     VRLTGQDSARGTFSQRHLVLHDVSTDATYTPLQQIPAARASFDIWDTPLSEASTMGFE
                     FGYNVEASESLVLWEAQYGDFVDSAQVIIDSFIVSARSKWGELSSLVLLLPHGYEGQG
                     PDHSSARLERFLQMAAEDNLRIANCTTAAQYFHILRRQAGLLKADPRPLILLTPKSLL
                     RHPMAVSPVRDLTEGRFQPVIDDPAAGAHPEAVRRLSLCSGRVYVELAAAGQKMEGGV
                     VDRSQVAIIRVEELYPFPESELQKVISGYPALEEVVWVQEEPRNMGAWPWVAPRLRDM
                     LEGKLPLLYAGRPRRASPAEGSHEWHVEEQKRMVEAAFLREAPILR"
     gene            9216..9974
                     /locus_tag="VHS06_04450"
     CDS             9216..9974
                     /locus_tag="VHS06_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX2987403.1"
                     /translation="MLAWGSRNRSATEYLSWRPFGLRILVASFIATLALLGIRSVALP
                     AMAAPAAAILNSPSCQTAPEEASLADEAAGITADEAQSPAVEPPVDEFATDPEAGDST
                     QERITPTSRGGARPSDSASPAADSQTRGDRIASIAQKYVGYGYRWAGMSPRTGFDCSG
                     LMGYVYQEAGISVPLHSLAGMLNSGKRVNRDQLRAGDMVFFENTWQWGLSHGGIYIGN
                     GEFVHAESEGVGVVISDLDAYNWISHFVGGSRPW"
     gene            10623..11816
                     /locus_tag="VHS06_04455"
     CDS             10623..11816
                     /locus_tag="VHS06_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2987404.1"
                     /translation="MKRTLTFAAILVLIATLSYVLLGCAPSPAASPTAQSGSTGTQKS
                     AASSDPIVIGLVTEVTGPQAGTGDNQTNGLKLAVDEWNQKGGINGRQIKLVIMDDTST
                     SPGAVNGYNKLISESKPAAVVIPNFANFDMAIEPAIRKAGIPAITAASAVGVTKAGNP
                     WIFRVRTNDTIMGQLAANYAAKEMGAKKVGILYVTGEMGAPASKVVQEELQKAGVTVV
                     GMESYNQDDKDVTAQLLNLQKAGAELVIGWSYPPDAAMIMTNMLQLGVKMKLLGSPAY
                     GIADCLKLAKDAANGHTVIMDWVPGDDATTQEWVKKIEGRAKLSANFIQSVYYDGGNI
                     LFQTIEKVGTDPAAIRDGLKAVKGYNGITGEYTFDAEGNGLRQAYIAQIKDGKPVLIK
                     TVKGQ"
     gene            11982..12857
                     /locus_tag="VHS06_04460"
     CDS             11982..12857
                     /locus_tag="VHS06_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2987405.1"
                     /translation="MDILLQILISGLAMGSIYGLVALGFVLLVNAADIINFAQGEFVM
                     LGAFLVFTFADLWGLPFLLSVLLVVVVMAIFGLVMERIAYRPLQNTNAVTIIISTLGI
                     SVFLQNVALRTWGSVPEYFGLPFGRAMIDLGSIRLVPQHLLILGVAACLVLAQHWFFH
                     NTRLGKLMRATAQDKQTARLMGVNIGLMTALTFAFASALGGLAGILLAPTFYISVSMG
                     VRVGIKAFVASIIGGWGSIPGAMAGGLLIGLVEVLAAAYISSLYKDVFAFLLLILFLV
                     VMPRGIFGEKVAQKV"
     gene            12858..14714
                     /locus_tag="VHS06_04465"
     CDS             12858..14714
                     /locus_tag="VHS06_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX2987406.1"
                     /translation="MRLTQIQKAGKGSIASKWAVVAALLVGVALPWLFPSNYNIVLLN
                     MALIFTLVVLGLNFVYGFGGIFSLAQAAFWGIGAYTSALLTTDAHMPFLVGLLGALVV
                     TSLFGIVLGVPTLKLRSHYLTMATLGFAEAVQLVLLNADRLTHGAQGVRGIPPASLGP
                     LVFDTPVTSYYLSLAVVAVAILFTRRFLGSRLGRALEATRDDELAAGATGVNVTYCRV
                     LAFVLSAFFAGLAGSLWAHSSSFISPDTFDLFTTIRFVAMLLIGGSGTAMGPVVGSLL
                     LTYLPEWLRFLEAYYMAIYGLSIVLILVFAPRGIVGLLQSLVECTLHNSATEPTSVVA
                     TQPDRAASPASSQPLTPIPHPPSPTLLSTRSLTIAFGGLEAVGGVNLSVAPSEIHGII
                     GPNGSGKTTLLNLISGIYRPTSGEIHLAGERVDGCGPTTLVHKGIARTFQNIRLFPKL
                     TVLDNVKVAKFCNTHSGLGGTMLRTGNTVAEERRVEEEAVAALEFVGLAHRAADLPGD
                     LPYGQQRLLEIARALCTNPKLLLLDEPAAGMSLGEKQRLVRLVQAINKDRGIAILVIE
                     HDMRIISGLCDQVTVLNFGKTIAEGAPMVVRDDEAVIEAYLGRRDRKRARSR"
     gene            14698..>15364
                     /locus_tag="VHS06_04470"
     CDS             14698..>15364
                     /locus_tag="VHS06_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2987407.1"
                     /translation="MLVLDSISVCYGKVEALHGISLEVHQGEIVALIGANGAGKSTTL
                     RAISGVLRPNHGQIHFDGKPISALTPERVAAAGIAHVPEGRRVFPGFTVAENLELAGL
                     ALGRPTQEIAHDVEMILEMFPRLRERYKQYAWSLSGGEQQMLVIGRGLVAKPRLLMLD
                     EPSLGLAPNLVDQVFDVIDEIRSRGTTILLVEQNASMALDLADRGYVMELGKIVLDGP
                     ATDL"
BASE COUNT         2906 a         4973 c         4507 g         2978 t
ORIGIN      
        1 gggagtatgt aggagggtcc gatacggatc ctgccacgcc ggatgagtcg agctagcctc
       61 gcctccgctt cgggacgtat ctcgaggtag tcctccagcg cgatgccctg cccgtcgaag
      121 gtgaagctgg agaaggacgg atcggcctcg aggacatcca tcagctcgtc ggtggttcgc
      181 accaggaaga ggcggaagag ttgaaacggg aagtaccact cgcgatccca gtgggtatgg
      241 ggcacgaccc agatgcggat gcgcgacctt tcgccggagc cggctccctc tgccggtgaa
      301 ttctggctct ccgcaagcgc ggccgggctc tccattctgc tggtgtcctc cttgactatg
      361 gcttacggat gggcagctgc ggcatgcacc accgctagaa cagttgcgtc aaattatgcc
      421 atagcatact gcgcaccgca tcgcagcgac cgcacaggaa cgatatccaa gaaatggaga
      481 ctggccgcca agagggcccg aggtgggcac ggctcatcgc gcctctgtgt gacggatctt
      541 gctctaggag ggcgggttgc ccctaacccg ccatctcgtc ggcggggttg gggcaacccc
      601 gccgacgaga tggcggagga cttgggcgat tccacacttg accgcgaaga gccgcgagca
      661 ccctttagtc gttatttctc attgaaatag cgtataatct cccgtgcggg ctcgcctatt
      721 cggcgtcgtg gggctgccgt ttcgcaccgt tcgtccccga ctcagcgccc gcgcaaacag
      781 gaggtacagg atgaagtcga gagtgacaaa gagtgacagg gagtggcagg aaaccttgac
      841 tcccgagcag tactacgtta cgcgaaggaa agggacagag cccccctatt caggaaagta
      901 caaccgcctc cacgacaagg gggtcttccg atgcgtctgt tgcggaaatg agctgttcag
      961 ttccgacgcc aaattcgagt ccaggaccgg atggcccagc ttcacggaac cgatctcgga
     1021 aggcagcgtg acgaccaggg aggaccgcag cctcttcatg gaacgcaccg aggtgctgtg
     1081 cgcgcgctgc gaggcccatc tgggtcacgt cttcgacgat ggtcccaggc caaccggact
     1141 tcgctactgc atgaactccg tggctctgga tttcgaggcc gaagatcagg aagagggctc
     1201 cagaggggaa ggctaggtct taggactacc gacggcccac atcagatcca agcaggctgc
     1261 gaggacctcg catccacgta gcccgcccca taccgcgagt tttgccccac ggctcaacgt
     1321 cgcctctgct cccgcttccg tttgcactcg atgcatagga cagccgaggg catcacttcc
     1381 agccgctccg gggcaatagg ccgaccacag ccgtcgcaga gaccatagct cccctcgtcc
     1441 accttctcca gtgcacgtcg cagttcgtcc atggcgcgcc ccatgttctg gacgactgcc
     1501 ctgttgttgg tggcctcctg cacctcggcg gtgtaatcac caacccgctt tccgaaccca
     1561 ggtcggttgc cctcggcccg gcgctcacgc tgagccgtgt cggccatctc cctgagatgc
     1621 tggttcagcc cgtccagctc agcctgaagt gcagccttga cggcagaaag tcccctattc
     1681 tcttgccctt ccattccgat ctccatatcc gaagcacccg ttatggatac cgggcgccgc
     1741 aactcccacg ccattctatc agagacagaa ggtgaccgac gaagagtcga ccagccctct
     1801 gcacgtcgtc ctcgccccat tgacgcgtcg ccaggcatac gctatgctaa tttcggtgtt
     1861 tggcgaaata tcgaaggagt gggagatggg cgtcaacgac gtgttccgag ccctgagcga
     1921 tcccaccagg cgagagatcc tcaggctgct gcaggccggg gacatgaccg caggggaaat
     1981 ctccgaccgt ttcccgctgg ccaggtccac cctctccggc cacttcaacg tcctgaagca
     2041 ggcggggttg atcgtcatgg agcgacaggg caccaggatc gtgtacagtc tggatacttc
     2101 tgccttcgat gaggcgatgg ccaccatact cgacctcttc ggagttggga aacaggaaag
     2161 gggcggcggc aatgcgcatc acgtggaaga ctgaatggcc caactgggtg ctgatactgg
     2221 gcatgttcgt tatggccggc ctctcctggg cgtcggcccc cgaccgcatt ccggttcact
     2281 ggaacatcac cggccaggtc gaccgctacg gcggcaaggt ggagggtctc ctggcgatcc
     2341 cgctcataac cctcagcacc tacatcctgt tgatcctcac gccgctcata gatcccggca
     2401 gggccaacta cgagcgcttc gcgggtgtct actccctgat ccggaccctg ctcacggtgc
     2461 tgttcgcggc aatctacgct gtggtcctgc tgtgggtccg gggctaccag gtcgacgtgt
     2521 cggcgatcat ccccatcgcc atcggcgcct tcttcatcat aatcggcaac tacatgggga
     2581 agatccgccc caactggttc gtgggcatca ggaccccatg gacgatctcc agcaagcgat
     2641 cctggacaaa gacccaccgc ctgggtggat ggatcttcat cctgatgggg ctggtactga
     2701 tcctgtccgg ggtggtgcgc accgaatggg ccgcgtacct gtccttcgcg gtcatcattg
     2761 gtggggtcat ctggaccttc gtctactcct acctggtctg gcgaaacgac ccggatcgaa
     2821 tccccccctc cggcacgctg cccggctgag cacgacggca ggctggcaac gcggctgcgg
     2881 ggacaaagcg cttcgttcca tcaacaggaa atgggagggt gtctcgacat gatcgcttcc
     2941 ttctccgaga ccgacaatcg cgtcaactgg aggcaggtgg gcgccttcgt ggcccttacc
     3001 ttcggtctca cctgggcgtt ggatgccgcc atcgcgttaa ccgtgggata ccgaagcccg
     3061 gcatttgtcc ttcttctcca gcttcagatg ctgatgccgg ccttcagcgc gattctgctc
     3121 ggcagcttct tcttcaccga tagtcccatc taccggcggg ttcccttggg tagccccagg
     3181 cacttcttca ccttcttcct tgtctacacc atcatctaca tgatcctggg atgggcgtcc
     3241 atcgccgctc cccagcagtc gcagctgttc agcactctcg gactggccct caccatcctg
     3301 ggactcctct tcattctatt gctgcgggtc cgcggcggac gacagcagtt cggcaccgcc
     3361 ggtctggctg ggggcaaccc tcgctactgg ctgctcttcg gcgtcgccgt cctgctcttc
     3421 tacagctttc aaaccctgct caacagtctg ttgggtctgg gccaccccgc ggacctatcc
     3481 gcgctgaccg gccaaaccgg aatgtccgat tcggtgttgc tcctgatcgc agccgtgcaa
     3541 tctgtggtcg tgggcccatt gatagcactg ccgattgcat tcggggagga gtacggctgg
     3601 cgtggatacc ttcagggtga gctggtcaaa ctgggcaaga tgaggggcgt cctgctcgtc
     3661 ggaaccatct ggggcctgtg gcacgcaccc atcatcgcca tggggcacaa ctacccgggc
     3721 cacccagtgg agggaatcct gctgatggtc gcttactgca tcggcctcgg ctacttcctc
     3781 gggttcgcgg ttctcaagtc cggcagcgtg tggctggcgg ccttcctcca tggtctcaac
     3841 aaccaagccc tgggctatct ctccgggctc atcaataccc caaacgatcc cgtctactcc
     3901 ttcggtgtcg ggatctacgg catggtaccg ctggcaatcg tgacgctgct gatcctgcgg
     3961 gatcatgttt ggaaggaatc gaccagggct ggaacagtga gcagccagct ctcgtgatga
     4021 gagccggcag tcccctgccg gcctacccct ccagaagcat cctggccggt tcctcgagca
     4081 attccttcac cttcaccagg aagagcaccg cctctcggcc atctaccacc cggtgatcgt
     4141 agctcaatgc cacatacatc attgggcgaa tggccatctg cccattcacc accacaggac
     4201 gctcggagat cttgtgcatc ccgagtatcc ccacctgcgg ggggttcagg atcggagtgg
     4261 acatcaacga tccaaaaacg cccccgttcg tgatggtgaa ggtgccccct ctcaactcct
     4321 ccagggttag ggatcgctcc ttcgatttct gcacgaagag ggctatcgcc ttctcgatct
     4381 cggcgaagct catccgatcg gcatccctca atacaggaac caccaacccc tcgggatccc
     4441 ccagcgcgat accgatgtcg tagtagtgct tcagcaccag ttcctcgccc tgaagctcgg
     4501 cattaatctc gggaaacgcc ttcagcgcac caaccacggc cttcacgaag aaggacatga
     4561 agccgagatc cactcccatt gccgccttga aggactcccg ttgccggcgg cgcaagtcca
     4621 tcaccgcgct catgtcgatc tcgttgaagg tcgtcagcat cgcggccgtg tgctgcgcct
     4681 ccaccaaccg cctggctata gtcagccggc gccgcgacat tcgctgcctg atctcctcgc
     4741 gttccccggc gacaggagag gggactgcgc ggacggctgc cgctggagct gtggccgttg
     4801 gcggggcagt cggagcgggg ggagtcggag cgggtgcggg cgagggtggc gcgcctgccg
     4861 cttcgcgctg tcgcgcaaca gcctccacat cctccttcat tatccgtccg ccggggccgc
     4921 tcccctgcac cgcggagaga tcgaccttca actccgctgc cagcttctgg gccacgggcg
     4981 atacgtgcgc cttctccacc tcccttacag gggagggagg cgcagcggcg gccgatggag
     5041 ccgccggttc gcgaggtggt gatggttgga cagcctgcgg ctcctgcggt ggtgcacctg
     5101 gggcaggtga tggagcccca agttccagcc tggccagtag gtcgccgggg tgcacggtgt
     5161 ccccctcgcc gtgcactatc tcactcaata caccgggttc ctcggcggca acctgcatgt
     5221 ttaccttctc agtctccaac tcaaccaccg gctccccggc cgcgacggac tcgccaacgg
     5281 atttcagcca ctttcccaca gtggcctcga ccacagattc gccaaggacg ggcacaacaa
     5341 tctcagccgt cacaactgac acaactgcac ctcaacgcaa gattggggct tcccgaagga
     5401 aggcggcctc aaccattctc ttctgctctt cgacgtgcca ttcgtgtgaa ccctcggcag
     5461 gactcgcccg ccgcggcctg ccggcgtaca ggaggggcag tttgccctcc agcatgtccc
     5521 tcaatcgtgg ggcaacccag ggccaggccc ccatatttcg aggctcctcc tggacccaca
     5581 ccacctcctc aagcgcagga tagccgctga tcaccttctg caactccgac tctgggaagg
     5641 gatacagttc ctccacccga attatcgcaa cctgggagcg gtcgacgacg ccgccctcca
     5701 tcttctgccc cgctgcggcc aactccacgt agacgcgtcc gctgcacagg ctcagacggc
     5761 gaactgcttc tggatgcgca ccggccgcag gatcgtctat gaccggctgg aacctgccct
     5821 ccgtcaaatc acggaccggc gacacggcca tggggtggcg cagcaggctc tttggcgtca
     5881 gcaggatgag tggtcgaggg tctgccttca gcaacccggc ctgccggcgc aggatgtgga
     5941 agtactgagc ggccgtcgtg cagttggcga tccgcagatt gtcctctgcg gccatctgaa
     6001 ggaagcgctc cagtcttgca ctggaatgat ccggcccctg cccctcgtat ccatgcggca
     6061 gcaacagcac cagtgaagac agctcgcccc acttggagcg agcggacacg atgaagctgt
     6121 cgattattac ctgggcagag tcgacgaagt cgccgtattg agcctcccac agcaccaacg
     6181 attcgctcgc ctcgacgttg tagccgaact cgaagcccat ggtcgacgcc tcggacagcg
     6241 gggtatccca gatatcgaag gaagctctcg ccgcagggat ctgctgcaga ggggtatagg
     6301 tggcgtcggt gctcacgtcg tgcagcacga ggtggcgttg gctgaaagta ccacgagcgc
     6361 tatcctggcc cgtaagccga accggcgtcc cctccgctgt cagagatgcg aatgccagct
     6421 gctcggcctg agcccaatcc actccaccct tctgcccctc ttcagccgag gaggcaaagg
     6481 cggaccggcg ccgattgtag atcggctcga gcttggagtt cagcttgaaa ccgggcggca
     6541 actccagcag ttgacggttc agctcagcca gcgtttccga cggcacccgg gtttccacct
     6601 cctgggcgac tccctggttt cccaagccgc ccctagtgcc gccggcctcc atccccgcct
     6661 gctggccgtc gcccgtggtc acgccatcca tagaaggcag ggatctccga gcggcctgga
     6721 gtctctccat agtcgcccgc accatcccct ccgcctcttc gggggtgatc gaccctcttc
     6781 gcaccagttc ctttgcccag agctccctca ccgccgggtg gccggagata accgcgtaca
     6841 tcgtcggctg tgtgtaggtc ggttcatccc cctcgttgtg gccccaacga cgatatccga
     6901 tgagatcgat gagcacgtcc ttgtggaagc gctccacgta ggcatgggca agtctcacgg
     6961 ccgcgatgca tgcctccggg tcgtccgcgt tcacgtgaac gatcggaacc tcgaacccct
     7021 tggcaggatc actggcaaaa agggtggatc ttccaagtct agacgaggtc gtgaacccaa
     7081 gctggttgtt ggctatgatg tggacgatgc cgccggtgtg gtagcccgga agcatggaga
     7141 ggttgagcgt ctccgccact atcccctccc ccacaaagga ggcatccccg tggatcagga
     7201 caccaagggt gatcctctcg tccagcctcg cggggcctcg ctggtccctc cgctcgctgt
     7261 tcgccctggt catcccaacc acgacaggat cgacatattc caggtggctc gggttggggg
     7321 ccatggtgac cagcattccc acctcacgcc cgcctcggaa ggctcgccga gcgccgagat
     7381 ggtacttcac atcgccggtc cagccgacgc cgctgctgtc ggtggctgag acgtttggcc
     7441 gctgcagcga gccgcggaac tccgccagga tctcctcgta cgacttgccc agcagatgag
     7501 ccaggatgtt cagccggccg cggtgagcca tgcccaacag caccgactgc gtaccgatct
     7561 cggcagccgc ccccatcaac tcgtccagca tggggagcag catgtccagc ccttccatgg
     7621 agaagcgggt ttgcgcggga aatgccgtgt ggaggaaacg ctcgaacgtc tccgcctggg
     7681 tcaatcgctc cagtagccac cgtgcgtcga tcaagtcctg ggggggacta taccgtcccg
     7741 actccacggc gttgcgcaac caacgccgct cctcggcgtt gtagacgtgc tcgaactcgt
     7801 agcctgtcgt cccctggtac acctgccgga ggcgagcgat cgcctctttc gcgctgcctg
     7861 cccgctccgc caccggccct ccaatcaccg acgggggcat cgccgacagt tgaccctcgc
     7921 gcaatccatg gttctccgcc agaagctcag gatcgcccgg cggctcgctg ccaagaggat
     7981 ccagtctagc tgccctgtac ccgtatatcc tgatggccgt actgagcgcc ctggccgccg
     8041 agatgaggtc gcaatccacc cctggagctg ccaccgtcgg agcaacctct cctgcctgaa
     8101 ccggcaaccg agggggaccc catcgatcga agaacgacct gatttcggga tcaacggatg
     8161 cgggatccgc actgtatcgc tcgaacagtt ccgccatgta ggcggcgttc agtccggaga
     8221 actcctccca tcctctgcca acgttgccgt ccatattcct ccaaccaacg ggggcatatc
     8281 accaccacac accagcctac tactttgtgt ttgctacaag tttagcacaa tactacttgc
     8341 ttcattctca actggtatct tcttgtgctg cgttcacctg atggcgaagc gcaccacggc
     8401 gcatcgagcg gctccggcta cacgatgtcc atggttccta cacggggcag atcagcgagc
     8461 cccgccacaa cggatggcgg tttgagaaac gagccgcact tcttctacat ccatgctacc
     8521 gtggacaact agttacgcaa aatcgaggcc agggacggag gcagtagagg cggacggaca
     8581 acgagtcgag cagcgggtga gggcatcaca gggcacatag tgatggccat ggattacgtg
     8641 atgtggcttc aggcaataca acaaacggag ggcgaagagt aatgagagac taccgaggtc
     8701 ggcaaatccc cacccccggt ccgcgagaag gcggaccggg ggtggggagt agttttcccg
     8761 tgtccatcta cctacatagt aattatcggg atcaatgatg ctcccggcca ttacccaaat
     8821 gggtaatatt ccttttcggg acggagacgc tgccgcgacc atcccacaca ctgacggcca
     8881 cgtctcgccg cgccgatcgg cgctttccta ggggattccc ctatttttcg ggacgcatgc
     8941 ccgttttcac cgcaatgcgc cgtaaccttg gtgcactcct atgctatact ccctcctcaa
     9001 gaaccaccta ccacccttcg tacccatttg gcgttgccgc cctccggccc atccgccggc
     9061 gcgggcgcgc atcacttcag cttcccgaca taaccagaac cgaagaacac cggtccctta
     9121 gcaggcgaac gcgcgcgcgg ggagtctgac gaatctggag gtcgagaatt accagcatag
     9181 ataacccacg tagacgccac ttctcgaggc cgggcttgct ggcctggggc tcgaggaacc
     9241 gatcggcaac cgaatacctg tcctggcgcc cattcggatt gcgaattctc gttgcgtcat
     9301 tcatcgccac ccttgccctc ctggggattc ggtcggttgc gctccccgcc atggccgccc
     9361 cggctgcagc catcctgaac tccccatctt gccaaacggc tccggaggag gcatccctcg
     9421 ccgacgaggc ggccggcatt acagcagacg aggcccagag tcccgccgtc gaaccacccg
     9481 tcgatgaatt cgcgaccgat cccgaggcag gagactcaac tcaggaaagg atcacgccca
     9541 cctcccgcgg tggcgcgcgc ccgtcggact cagcgtctcc cgcggcagac tctcagacta
     9601 gaggcgatcg cattgcctcc atagctcaga aatatgtcgg ctacgggtac cgatgggccg
     9661 gcatgtcacc gcgcacgggt ttcgactgca gcggtctgat gggatacgtg tatcaggaag
     9721 ctgggatcag cgttcccctt cacagcctgg ccggcatgct caatagcggc aagagggtaa
     9781 atcgtgatca actgcgagcc ggcgacatgg tgttcttcga gaatacctgg cagtgggggc
     9841 tgtcccacgg cggcatctac atcggcaacg gagagttcgt tcacgcagag agcgaaggtg
     9901 tcggggtagt gatcagcgac cttgacgcct acaactggat ctcccacttc gtgggtggct
     9961 cgcgcccctg gtgagtcccc acgtcgggat atgcacatcc cttacgtacc gtcatgctga
    10021 gcgacagcga aggatctcac cgttcacgag gagatgcttc gcttcgctca gcattacaga
    10081 ctgtagtgat cgctacggca cagaaatcat ccccacatcc ccgcatcccc gtgtctccgt
    10141 gtctccgtat ccccgcgcct catagttgac acctctgggt cttcgtgcca tactgccgct
    10201 caagtttcat agtccatcgt cgatgaacgg gaatagtaag cctccagcga gcctcagcga
    10261 gttgggtaga gtgtgagcca acggtagcgc agcggcggaa tggacccggg agacgcagcc
    10321 cgaaaccgag aagggtagat ggatcggtcg tcggtccatc cacgagagat gaactcggga
    10381 gtagtagctg ccggagactc caccgttagc tgggagaggg catcggatcg tcgcgcgagg
    10441 tcccgtgcct gcagagcgtg gcccgcagga ttacttttcc tggcggccaa actagggtgg
    10501 taccgcggag cacccctccg tccctttgtc ggaccgaggg gtttttgatt cccctcgggt
    10561 ttcaggccgg gggtgccggc catctggcat cccgatccgt gatttaccga gggagaaacg
    10621 gaatgaaaag gacactcacc ttcgcggcaa tacttgtcct catagctacc ctttcgtacg
    10681 tgcttttggg atgcgcacca tcccctgccg cgtcgcccac agcacagtcc ggctcgaccg
    10741 gtactcaaaa gtcggctgcc tcaagtgatc caatcgtcat tggcctcgtc actgaagtca
    10801 cgggtcctca ggcaggtacc ggtgacaacc agacaaacgg cctcaagctc gccgtggacg
    10861 agtggaacca gaagggcggg atcaatggcc gccagatcaa gctcgtgata atggacgata
    10921 cctccacgtc accgggtgcc gtaaacggct acaacaagct catctctgag tcgaagcctg
    10981 cggcggtggt cattccgaac tttgccaact tcgacatggc gatcgagccg gccataagga
    11041 aggcggggat acccgctatc accgctgcca gcgccgtcgg cgtaaccaag gcaggcaatc
    11101 catggatctt ccgtgtgcgc accaacgaca ccataatggg ccaactggcg gcgaactacg
    11161 cggccaagga gatgggcgcc aagaaggtcg gcatactgta cgtaaccggc gagatgggtg
    11221 cacccgcatc taaggttgtc caggaagaat tgcagaaggc aggcgtcacc gtcgtcggca
    11281 tggaatccta caaccaggac gataaggacg tcaccgcaca gttgctgaat ctccagaagg
    11341 ccggcgctga gttggtcatt ggatggagct acccccccga tgcggcaatg ataatgacca
    11401 acatgttgca gctcggcgtc aagatgaaac tgctgggctc tcccgcttac ggcattgccg
    11461 actgcctcaa gctggccaag gacgccgcca acggccacac cgtcatcatg gactgggtcc
    11521 ccggtgacga cgcgacaacc caggagtggg tcaagaagat cgagggccgg gcgaagctct
    11581 ccgccaactt cattcagtcg gtctactatg acggcggcaa catcctgttc cagaccatag
    11641 agaaggtcgg aaccgaccct gcagccatcc gcgacgggtt gaaagcggtg aagggctaca
    11701 acgggatcac gggcgagtac accttcgatg ctgagggcaa cggtcttcgc caggcctaca
    11761 tcgcccagat taaagatggc aagcccgtgc tgatcaagac ggtcaaaggc caataaccgg
    11821 gggccaggtg tcgggtccca ggggccgggg aggaattgga gatcgggtgt tgagggattg
    11881 gagcaaggag tctatcactt ccatcccgta agccctcaac ctatctgcca gttccccggc
    11941 ccccggtccc cggtccctga caccctgcaa cgggggggca cttggacatc ctgttacaaa
    12001 tcctcatcag cggattggcc atgggcagca tctacggatt ggtggccctc ggcttcgtcc
    12061 tgctggtgaa cgcagccgat atcatcaact tcgcccaggg cgagttcgtt atgttgggcg
    12121 cgttcctggt gttcaccttt gccgacctat ggggactgcc gtttctactt tcggttctgc
    12181 tggtagtggt ggtaatggcg atcttcggcc tggtaatgga gcgaatagcc tacaggccat
    12241 tgcagaacac caacgccgtc accatcataa tcagcaccct gggcatctcg gtgtttctcc
    12301 agaacgtggc tctgcgcacc tggggctcgg tcccggagta cttcggcctc cccttcggtc
    12361 gggcgatgat cgacctcggt agcatccgcc tggttcccca gcatttgctg atcctggggg
    12421 tagctgcctg cctggtgctg gcgcagcact ggttctttca caacacacga ctgggcaagc
    12481 tgatgcgggc aacggcccag gacaaacaga ccgcaaggtt gatgggggtc aacattggcc
    12541 tgatgacggc tctcaccttc gctttcgcct cggcgctggg agggctagcc ggtatcctcc
    12601 ttgcgccaac cttctacata tcggtgagca tgggcgttcg agtcgggatc aaggcattcg
    12661 ttgcgagcat catcggcggg tggggcagca tccccggagc aatggccggc ggcctcctga
    12721 tcgggctggt cgaggtgctg gctgcggcct acatctcctc gctctacaaa gacgtcttcg
    12781 ccttcctgct gctgatcctc ttcctggtcg tcatgcccag gggaatcttc ggcgagaagg
    12841 tggcacagaa ggtatagatg cgtctgactc agatccaaaa ggcaggtaag gggagcatag
    12901 cgagcaagtg ggctgtcgtc gcagccctgc tggtgggcgt cgccctaccc tggctcttcc
    12961 cctcaaacta caacattgtc ctgctaaaca tggccctcat cttcaccctg gtagtcctcg
    13021 gcctgaactt cgtctatggg ttcggaggga tcttctccct cgcccaggcg gccttctggg
    13081 gtattggggc ctatacttca gccctgctca cgacggacgc ccatatgccc ttcctggtcg
    13141 gactgctggg agcacttgtg gtcacatccc tgttcggcat cgtgctgggc gtgcccacgc
    13201 tgaaactgcg cagccactac ctcactatgg caacgctggg cttcgcggag gcagtccagt
    13261 tggtgctgct gaatgcggat cgcctcaccc acggcgctca gggcgtcagg ggcatcccgc
    13321 cggcgtccct agggccgctg gtcttcgaca ccccagtgac ctcctactat ctgagtctgg
    13381 ccgtggtggc cgttgcgatc ctgttcacca ggcgcttcct ggggtcccgt ctgggcaggg
    13441 cgctcgaggc caccagggac gacgaactgg cggcaggggc aaccggcgtg aatgtgacct
    13501 actgcagggt gctcgccttt gtgctcagcg ccttcttcgc cggcctagct ggaagcctat
    13561 gggctcacag ttcatccttc atcagtcccg atacattcga cctcttcacc accatccgct
    13621 tcgtcgcaat gctgctcatc ggcgggtcgg gaacggccat ggggccggtg gtggggtcgc
    13681 tgctgctgac ctacctgccg gagtggctcc gcttcctgga agcctactac atggcaatct
    13741 acggcctctc catcgtcctg atcctggtct tcgccccacg ggggatcgtc ggcctgctgc
    13801 agtcgctggt cgagtgcact ctgcacaact ccgccacgga gcctacctcc gtcgtcgcta
    13861 cgcaacccga tcgcgccgcc tcgccggcat cctcgcagcc cctaaccccc atcccccatc
    13921 ccccatcccc cactctgctc tctacccgca gcctgactat cgctttcggg ggtctcgagg
    13981 ccgtgggtgg ggtgaacctc tcggtcgccc cgagcgagat ccatgggata atcggtccca
    14041 acgggtcggg caaaaccacc ctgctcaacc tcatcagcgg gatctacagg cccacgtcag
    14101 gagagatcca cctggcgggt gagcgggtgg atggttgtgg cccgactacg ctggttcaca
    14161 aagggatcgc tcgaaccttc cagaacatcc gtctcttccc aaagctgacc gttctggata
    14221 acgtcaaggt ggccaagttc tgcaacacgc actccggcct cggaggcacc atgctgcgga
    14281 caggcaacac ggtagccgaa gagcgccgag tcgaggagga ggctgtggcg gccctggagt
    14341 tcgttggatt ggcccatcgt gccgccgatc tccccggcga cctcccctat gggcaacaac
    14401 ggctgctgga gatagcccgc gcgctctgca ccaacccaaa gctgttgctt ctggatgaac
    14461 cggccgcagg catgagtctg ggtgaaaagc agcgactggt gcgactggtg caggccatca
    14521 acaaagatcg cggcatcgcc atcctggtga tcgagcacga catgcggata atcagtggcc
    14581 tctgtgacca ggtcacggtg ctcaacttcg gcaagaccat cgccgagggg gcccctatgg
    14641 tcgtgaggga cgacgaagcc gtcatcgagg cctatctcgg aagaagggac agaaaacgtg
    14701 ctcgttctag atagcatctc tgtttgctat gggaaggtag aggcacttca tgggatctcc
    14761 ctcgaagtac accaggggga gatcgtggcg ctcatcgggg ccaatggcgc aggaaagagc
    14821 accacgctcc gtgcaatatc cggcgtgctc cggccaaacc atggccagat ccatttcgac
    14881 gggaagccga tttcagcgct caccccggag agagtcgccg ccgccggcat tgcccacgtc
    14941 ccagagggca gacgagtgtt tccaggcttc acggtggcgg aaaacctcga actggcgggg
    15001 ctggcactgg gccgccccac tcaagagatc gcgcacgacg ttgagatgat ccttgagatg
    15061 ttccccaggc tgagggagcg atacaagcag tacgcgtggt ctctctccgg aggcgagcag
    15121 cagatgctgg tgatcggccg cggcctcgta gccaaaccac gactcctgat gctggacgag
    15181 ccatcgctcg ggctcgctcc caacctggtc gaccaggtct tcgacgtgat cgacgagatc
    15241 cgatccaggg ggacgaccat cctgttggtg gagcagaacg ccagcatggc gttggacctg
    15301 gccgaccgag gatacgtgat ggaactgggg aagatcgtgt tggacggacc tgccacggat
    15361 ctcg
//
LOCUS       DATALS010000199         4325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_20962,
            whole genome shotgun sequence.
ACCESSION   DATALS010000199 DATALS010000000
VERSION     DATALS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20962"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VHS06_04475"
     CDS             <1..929
                     /locus_tag="VHS06_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987408.1"
                     /translation="AVKAEVDALKGHVWLESREGLGTKVTMELPITLAFTHVLLIEVG
                     RGIFAVPCASTQGITEVPATAVRTLQSRESADLAGRAVPLVWAHNLLGGERPEIVGSR
                     FPALLMGSADRPIAFVVDRVVGDENVIVKPLGNLLRQVPNVSGGIILGDGRVALLLSV
                     GALVDAARGVVSAQPVVQKRSVKLTRRGRRVLVVDDSAITRELERSILATAGYDVETA
                     VDGQDALDRLHSREYALVVADVEMPRLDGFGLAGAIRSDPSLEKLPVVIVSSRESDED
                     RRRGMELGVQAYVGKGSFDQMTLLDTIESLIG"
     gene            1042..2544
                     /locus_tag="VHS06_04480"
     CDS             1042..2544
                     /locus_tag="VHS06_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX2987409.1"
                     /translation="MGSSSGIKNSKMGMTRAEYLLFQELIGQNSGLRLEDRDWTPLRG
                     AVFERVAAVGCSTLGEYYEMMRDPAGSLELGRLIELLTVHETAFFRNRLHFDLLIRHI
                     LPELMEKGDRSRPIRVWSAGCSTGQEAYSIAIALLESPLLSESVEFEVLATDISNRAL
                     DIARAGTYPPRALRNLDPQLLDRYFSPCDGGYEVNDRVRSRVRFEYLNLVKEPFPLAA
                     LSPFDVVFCENVTIYFKVESTRRVIKNFHDVLGDGGYLFLGYSETLWQIPNEFLLREL
                     DGTFVYQKSLGAEVSPPASIGTVAAGSALTTSAAPKPGAKPAARPVRSVPPAVARPPR
                     RRYSIEEATALYEGRCFEEALLAVESVLSTSPNDRRAHLLAAKLRADREEVGPALRHC
                     RRALDSDPLLEEGHYLMGVLQMRAGEYDSAVESLSRVIYLNSSGHRSALAHFHLAGLH
                     ADVERWELAAREYRNALRLLERFPKEELVGEFSADFLAGVCRRRLQELGQ"
     gene            2541..4040
                     /locus_tag="VHS06_04485"
     CDS             2541..4040
                     /locus_tag="VHS06_04485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2987410.1"
                     /translation="MTKLEAGMARARVLVADDSQTSLALVRDFLERSGYEVVTARDGL
                     EAIEAVYRENPDLVVLDVVMPRMTGYQVCRMLKLDEQTQHIPVVMLTSRDQPADQYWG
                     LQTGADCYVTKEQPLPYVLEAVGKVLAQRPERDGAAASSLGGSPFTAVDLLSRVNDIL
                     DRKLLEATILGEVTKLAWSATDLDKTTEEIMTLFAKLFDFSLACMLVRGDNSREGELV
                     WMIRSSIASGDLSACEDALYSSQGERSGAVIDRRAVKRRIVVAPGARVGADVKDPHPV
                     GRYYTVPLGSREQEAGVFAIWGGERTLESEESLQMISMLANAAYLVLDNARLYQEMRR
                     LATTDELTGLANYRQFEDALEREFQRSLRTGEPMAVLMMDLDNFKGINDVFGHKMGDT
                     VLHAVGSAFMGSARQYDIIARYGGEEFAVILPGTGARGAIEMGERLRLLVRTVGGRLK
                     LEKLSTSVGVALYPNGKVGDAQDLVREADAALYRAKAEGKDRVWLAAIS"
BASE COUNT          798 a         1146 c         1481 g          900 t
ORIGIN      
        1 atgcggtgaa ggcggaggtc gatgccttga aggggcacgt ctggctggag agccgcgagg
       61 gtctcggcac caaggtgact atggagttgc ccataaccct tgcctttacc catgttctgc
      121 tgatcgaggt gggccgtggg atcttcgcgg taccctgtgc ctccacacag gggatcacgg
      181 aggtgccggc cactgcggtc cgcacgcttc agtcacgcga gtcggcggac cttgccggaa
      241 gggcagtacc gctggtttgg gcgcataacc ttctgggggg tgagaggccc gagatcgttg
      301 ggtcgaggtt cccggctctc ttgatgggat ccgccgaccg gccgattgcg ttcgtggtcg
      361 atcgagtcgt aggcgacgag aacgtcatag tcaagccgtt ggggaatctc ttgaggcagg
      421 tgcccaatgt ttcagggggc atcatcctgg gagatggaag agtggctctg ttgctctcgg
      481 taggggctct ggtggatgcg gcccgcggcg tggtatcagc acaaccggtc gtgcagaagc
      541 gctcggtgaa gctgactcgt cgagggaggc gggtcctggt agtcgatgac tcggcaatca
      601 cgagggagtt ggagcggagc atattggcta ccgcaggcta cgatgtggag acggcggtgg
      661 acgggcagga tgccctggac aggctgcaca gtcgggaata cgccctcgtg gtggcggacg
      721 tggagatgcc tcggctggac ggtttcgggc ttgcgggggc gattcgcagc gatccgtcgt
      781 tggagaagct gcccgtggtg atcgtcagct ctcgcgagag cgatgaggac cgaaggcgag
      841 ggatggagtt gggcgtacag gcgtacgttg gcaaagggtc attcgatcag atgacgctgc
      901 tggatacgat cgagtcgcta atagggtagg tgttggccga ccgtggtcgc tttgtggacg
      961 tgagtgcgag agggcttgca gcgcaagtga tcggcgaaat gcttccgctg ggtgacgttg
     1021 ccggcagact ggtggattgg attgggcagt agttcaggga tcaagaattc caagatgggt
     1081 atgacgaggg ccgaatacct cttgttccag gagttgatcg gtcagaacag tggactgcgg
     1141 ctcgaggatc gggactggac tccgttgagg ggtgctgtgt tcgagagagt ggccgcggtt
     1201 ggttgctcta ccctgggcga atactacgag atgatgcgcg acccggcagg cagcctggag
     1261 ttgggccgtc tcatcgagtt gctgacagtc catgagacgg ctttcttccg gaatcggctc
     1321 cacttcgatc tgctcatccg gcatatcctc cccgaattga tggaaaaggg ggataggtct
     1381 cgcccaatcc gtgtttggag cgccggttgc tcgacggggc aggaagctta ttccatcgcc
     1441 atcgcattgc tggagagccc gcttctctcc gagtcggtag agtttgaggt tctggcaacc
     1501 gacatcagca atcgggcact tgacatagct agagccggga catatccacc tcgagccctg
     1561 cgcaacctgg acccgcagtt gctggatcga tacttctccc cctgtgatgg tgggtatgag
     1621 gtgaacgacc gagtcaggag ccgtgtcagg ttcgagtact tgaacctggt caaagagccg
     1681 tttcctctgg ctgccctcag ccccttcgac gtagtgttct gcgagaatgt gaccatctac
     1741 ttcaaggtgg agtcgacacg cagggtgatc aagaacttcc acgacgtcct gggcgacggc
     1801 gggtacctct tcctgggcta ttccgagacg ctgtggcaga tccccaacga attcctgctt
     1861 cgggaacttg acggcacctt cgtgtatcag aagtcgcttg gagccgaagt atccccgccg
     1921 gcttcaatcg gcaccgttgc tgccggctcg gctctaacga caagtgctgc gcctaagccc
     1981 ggtgcgaagc cggctgcgcg cccggtcagg agtgtgcctc ctgccgttgc gcgtcctcct
     2041 cgtaggagat actccatcga ggaggccact gctctgtacg agggaaggtg ttttgaagag
     2101 gctttgctgg ccgtagagag cgttctctcc acatctccga acgatcgaag ggcgcacctc
     2161 ttagccgcga agcttcgggc cgatcgcgag gaggttggac cggcactgcg gcactgccga
     2221 agggcgttgg actccgaccc tctgttggag gagggacact acctgatggg tgtcctgcag
     2281 atgcgtgcag gggagtacga ctcagccgtt gagtcgctct cgcgcgtgat ctatctgaac
     2341 tcatccgggc atcgctccgc gcttgcccac tttcacctgg cggggctcca tgccgatgtc
     2401 gagaggtggg aattggcggc cagagaatac cgcaacgctc tcaggctgct cgagcgattt
     2461 ccgaaggaag agttggtggg ggagttctcc gccgacttcc tggcgggggt gtgccgcaga
     2521 cggctgcagg agcttggaca gtgactaagc tggaggctgg tatggcaagg gcaagggtgc
     2581 tcgtagctga tgacagccag acatccctgg cgctggtgcg agatttcctc gagcggagcg
     2641 gatacgaggt ggtgacggcg cgtgatggtc tggaggcgat tgaggctgtt tacagggaga
     2701 acccagatct cgtcgtactg gatgtcgtca tgccacggat gaccgggtac caggtatgtc
     2761 ggatgctcaa gctggacgag cagacccagc atatcccagt ggtaatgctg acctccaggg
     2821 atcagcccgc ggaccaatac tggggccttc agacgggcgc ggactgctat gttacgaagg
     2881 agcagcctct cccctacgtg ctggaggcgg tgggaaaggt actggcccag cggccggaga
     2941 gggatggagc ggcggcatcc tctcttggag gaagtccctt caccgccgtc gatctgctca
     3001 gtcgggtcaa cgacatcctt gataggaagc tgttggaagc cacgatcctg ggcgaagtca
     3061 ccaagcttgc ctggtccgcg accgacctgg acaagactac cgaagagata atgaccctct
     3121 ttgccaagtt gttcgatttt tcccttgcct gcatgctggt tcgcggggac aactcccgtg
     3181 agggggagtt ggtgtggatg attcgctcat ccatcgcgtc gggggatctg tctgcctgtg
     3241 aagacgccct gtattcctct cagggtgagc gaagcggcgc tgtgatcgat aggcgtgcgg
     3301 taaagcggcg gatcgtcgtg gccccaggtg ctcgggtcgg tgccgacgtg aaggaccctc
     3361 acccggtggg gcgttactac acggtgcctc tgggctcccg cgagcaggag gcgggcgtct
     3421 tcgccatatg gggtggggag cgtaccctgg agtctgagga gtcgctccag atgataagca
     3481 tgctcgccaa tgctgcgtac ctcgtgctgg acaacgctcg cctctaccag gagatgcgcc
     3541 ggctcgctac cacggacgag ttgaccggcc tggcgaacta cagacagttc gaggacgcgc
     3601 tggagaggga gttccagcgg agcctgcgaa ccggcgagcc tatggccgtg ctcatgatgg
     3661 atctggacaa cttcaagggc atcaacgacg tcttcgggca caagatgggc gacacggtgt
     3721 tgcatgccgt gggatccgcc ttcatgggga gtgcgcgaca gtacgacatc atcgctcggt
     3781 acggcggcga ggagttcgcc gtcatcctgc cggggaccgg tgccagaggg gccatcgaga
     3841 tgggcgagcg gttgagactg ttggtgcgta cggttggggg gcggctaaag ctggagaagc
     3901 ttagcaccag cgtcggggtt gccctctatc ccaacggcaa ggtcggcgat gctcaagacc
     3961 tggttcggga ggcggatgcc gcgctgtacc gagcgaaggc ggaaggaaag gatcgcgtct
     4021 ggttggccgc gatcagttag cttccggcct gactctcctc tgactctccc attgaagggg
     4081 cgcgtgtccc cttgagtcgc gtcctcgtcg gaggatgcat gcccccagtg ggactcaact
     4141 acgtacagcc aacatttgtg ccatctttca cgcacaatat tttcgttatg gttgatcggc
     4201 gccgtggtta tgtgtaaaaa atgctcttgt gcccgccatg ggatgatcgg gtgcaaggcg
     4261 taatctgtga tctgatcacc cgatccacga tacagccggt gatctgatga tcggatctag
     4321 aaagg
//
LOCUS       DATALS010000200         5979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_202188, whole genome shotgun sequence.
ACCESSION   DATALS010000200 DATALS010000000
VERSION     DATALS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202188"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..1259)
                     /locus_tag="VHS06_04490"
     CDS             complement(267..1259)
                     /locus_tag="VHS06_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2987411.1"
                     /translation="MIARFTRAPQRLERFGLDTDRRRILYAFGAAILLFLLGELVHPG
                     FAAFKSVESILIIASFVGLVAAGQTFVILIGGIDLSVPWVLNGAAILMVTSSLGKDEN
                     ALYAIAVTLGMGLAVGLLNGLGIAFLSVPAVVMTLGMNGLMEGLTLGLSDGMTCHTCA
                     SYAPPVVRNTVHGNLFGISTDILFWIAVTLLVSFVLSYTVFGRRTYALGNNPQASYLA
                     GINVKLLTVALYMLSGMFSALAGIALVGFGGQASLGMGDPFLFQSIAAVVIGGVYILG
                     GRGHYLGTAAGAVTLVALLTVLMAVNMPEYGRNIIYGVVILLLLLLYGRQEQET"
     gene            complement(1256..2206)
                     /locus_tag="VHS06_04495"
     CDS             complement(1256..2206)
                     /locus_tag="VHS06_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2987412.1"
                     /translation="MQLASQNAPLLLALLILLATLALYGGLFFVAQGSIPGQFEVTQI
                     INNSMPLAVAAVAQTIIVLTGGIDLSVGGMMDLSNSLAAVNIQDNPASMVGWTIIILL
                     VGAAGGLANGLLVVKGRLQPILVTLATSAIFQGLAIRVLPQPGGSIPRSYTSLLVNPA
                     APTGVLAVALLILIWMLFTRTPFGVGVYAIGNDEQAARANGVPVQLTKVGAYVMSGVM
                     VAAAGIFLAANATAGDATSGNVFTMTSIAAAVLGGVNLAGGRGSAIGAVAGAFTLTIL
                     INVLFFAHIDPLYQSFYQGLFLILAVIISTLIGVFLRRRR"
     gene            complement(2232..3755)
                     /locus_tag="VHS06_04500"
     CDS             complement(2232..3755)
                     /locus_tag="VHS06_04500"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX2987413.1"
                     /translation="MIAFDKAIPAIEGLNVFKAFGAVQALDGASFQADFGEVHALVGE
                     NGAGKSTMIKILSGLIHPDSGVIRVRGQQVELSSPQAAQSLGIGTVFQELTLMPWMTV
                     AENLLLRHEPRNGLGLIRRGQMGHHAEEILAGLGIEQIDPMELVANLSLAQQQIVEIA
                     HAVIRDPDILFMDEPTSSLAEREVIWLFDLIKGLRQRGKCIIFTSHRWKEIESIADRI
                     TIFRNGVHVGTYTGLDEDQGITLMTGRKVDAMYPELAPRQSSKVVLEAKELTAPGVHG
                     ISFKLHQGEILGVGGLAGHGHRELFMTLFGVSKPTHGHILVDGKQVAIHSPRDAINAG
                     LGIALIPEDRKREGLLLPMSVRDNLTLAILDRVSALGVVRQANEQRLAGDMVAKLQVR
                     MGSPRRPVRTLSGGNQQKVLIGRWLLADSRVLLLYDITRGVDIATKHDIYDLMLQLID
                     EGRSIVFYSSDTEEIARLSHRVLVLREGRLSAELEGPGIDAEEIVAASIRENGTVKV"
     gene            complement(3791..5032)
                     /locus_tag="VHS06_04505"
     CDS             complement(3791..5032)
                     /locus_tag="VHS06_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX2987414.1"
                     /translation="MAKAYTRRYGDIRTLLRRSLIPLTAIAMLVAVAGCQSSPAPTAP
                     AAPTTAAASQAVAPATAAAPPAVAATTAPAAQAQPAAGKKLKIALANSYIGNKWRIEM
                     ANVFRAALDMEPYKSEVDGSIYSSGNDVSKQSQQLSNLISQKVDAIVLNAASPTGLNG
                     IVEQAAQRGILVVSFDNTVTTPAALKVNTDQFKFGAQLAQFLADKMGGKGNVIMVTGV
                     AGTTVDEQRNAGADSVWAKYPDIKVVNRFTGMWDSSVAERNAAGVLPSLPKIDGIWCQ
                     GGTDGVIKAFIAAGRPVPVVAGEAENGFRKFMLGDYMGQPKITAMSIGQPPFLSVVSL
                     ELARAILQNRYPKKDIEIPFPYVTTDTVKSGETVFPELEDSFFTDFTDSGDNATVQLC
                     VDAGLSGTACDGSLKVNLPKP"
     gene            5485..>5979
                     /locus_tag="VHS06_04510"
     CDS             5485..>5979
                     /locus_tag="VHS06_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2987415.1"
                     /translation="MDVQQIIAALNLQPHPKEGGYFVESFRSEGRLLAEVLPEGFSTP
                     RALSTAIYYLLTPATYSEMHRLPGDEIFHFYMGDPVEMLQLWPDGTGKVVELGTDLES
                     GQRPQVVVPGEVWQGSRLKQGGSYALLGTTMSPGFDYEDYESGQRTKTAISSKGGETY
                     KRVEK"
BASE COUNT         1180 a         1839 c         1858 g         1102 t
ORIGIN      
        1 actctcgtca agacgcccaa gatccctcca gaacagatca cccccttgcc aagagatatc
       61 atcctggtcc gtaagcccag atatggcaat tggaatcgta gctccagacc caccaaaccg
      121 aattagttgg aacagatggg cgtgaccgcg acgctacgcc cgctaccgtc gcgtgctcgc
      181 tcgcgtcacg agcccgacgc tacactgtgt gccgcccggt ccacgattcg gggcgggtcg
      241 gtggcgacgc gccctacgtc aattcgttac gtctcctgct cctgccggcc gtataggagc
      301 aacagcagca ggatgacgac gccgtagatg atgtttcggc catactcggg catgttcacc
      361 gccatcagca ccgtgagcag cgccaccagc gtcacggcac ctgcggccgt gcccaggtag
      421 tggccgcgcc cacccaggat gtacaccccc ccaattacaa ccgcggctat ggactggaac
      481 aggaagggat cgcccatccc gagggatgcc tgtccaccga accccaccag cgcgatgccc
      541 gccagggcgc tgaacatgcc gctcaacatg tagagggcga cggtcagcag cttcacgttg
      601 atccccgcca ggtagctggc ctgggggttg ttgcccagcg cataggtgcg ccggccgaag
      661 acggtgtagc tgagaacgaa gctgaccaac agcgtgaccg cgatccagaa caagatgtcg
      721 gtagagatgc cgaagaggtt gccgtgcacc gtgttccgaa cgaccggcgg cgcgtaggag
      781 gcgcaggtgt ggcaggtcat gccgtcgctg agccccaggg tcagcccctc catcaacccg
      841 ttcattccca acgtcatgac gacggcaggt accgagagga atgcgatgcc cagaccgttg
      901 agcagtccta ccgccagccc catcccaagt gtcacggcta tcgcgtacag cgcgttctcg
      961 tccttgccga gggaagaggt caccatcagg atggccgcgc cgttcagcac ccagggcacc
     1021 gacagatcga tgcctccgat cagaatgacg aaggtctggc cggccgctac cagtccgacg
     1081 aaggaggcga tgatgagtat ggactcgacg ctcttgaagg cggcgaagcc cgggtgcacc
     1141 aactctccca gcaagaagag caggatggcc gccccgaagg catacaggat ccggcggcgg
     1201 tcggtatcca gcccaaacct ttccagccgc tggggcgcgc gagtgaatct agcgatcatc
     1261 tacgcctcct gaggaacaca ccgatcaggg tgctgattat cacggccagg atcaggaaga
     1321 gcccctgata gaaggactgg tacagcggat cgatgtgggc aaagaacagc acgtttatca
     1381 ggatggtcag ggtgaaggca cccgcgacgg ccccaatagc gctgccccgt ccgccggcca
     1441 ggttcactcc accaagcacc gcggccgcta tggaggtcat ggtgaagacg ttaccggagg
     1501 ttgcatctcc cgccgtggcg ttggcagcca ggaatatgcc ggccgccgca accatcactc
     1561 cgctcatgac gtaggccccc accttggtca gctgtaccgg tacgccgttg gctcttgccg
     1621 cctgctcgtc gttgccgatg gcgtagacgc caaccccgaa gggggtgcga gtgaacagca
     1681 tccagatcag gatcagcagc gctacggcaa ggacgccggt cggggcagcg ggattgacga
     1741 gcaagctggt gtaggagcgc ggtatggaac cgcccggctg cggcagcacg cggatcgcca
     1801 gcccctggaa gatggcgctg gtggccaggg taacgaggat cggctgaagg cgccccttca
     1861 ccaccagcag cccgttggcc aggccgccgg cggcgcccac cagcaggatg atgatcgtcc
     1921 acccaaccat gctggccgga ttgtcctgaa tattgacggc ggccagactg ttggacagat
     1981 ccatcatgcc gccgacagag aggtcgatgc cgccggtcag cacgataatg gtctgggcga
     2041 cggcggcgac cgccaacggc atcgagttgt tgatgatctg cgtaacctcg aactggccgg
     2101 gtatcgagcc ttgggcgacg aagaagagcc cgccgtacag ggcaagcgtg gccagcagga
     2161 tcagcagcgc cagcaggaga ggggcgttct gagaggccag ctgcatccgg cgggcgtgag
     2221 agctgcgagg cctaaacttt gacggtaccg ttctctcgga ttgaagcagc aacgatctcc
     2281 tccgcgtcga tgcctggccc ctccaactcg gccgaaagcc tgccttcgcg aaggaccagc
     2341 actcggtggg agaggcgggc gatctcttcc gtatcgctcg agtagaagac gatggatcga
     2401 ccctcgtcga tcagctgtag catcaggtcg tagatgtcat gcttggtggc gatatccacc
     2461 ccgcgagtga tgtcgtagag cagcagaacc ctcgagtcgg ccaggagcca acgtccgatc
     2521 agtaccttct gctggtttcc accgctcagc gtcctcacag gtcggcgagg gcttcccatc
     2581 cgcacctgga gcttggcgac catatctcca gccagccgct gctcgttcgc ctgccgcacc
     2641 acgcccagag cggataccct gtcaaggatg gcaagggtga ggttgtcacg aaccgacatg
     2701 ggaaggagga ggccctctcg cttgcgatcc tcagggatca gcgcgatgcc cagccccgcg
     2761 tttatggcgt cgcgggggct gtggatggcg acctgcttgc cgtccaccag gatatggccg
     2821 tgggtcggct tgctgacgcc aaaaagggtc atgaacagtt cgcggtggcc gtgccctgcc
     2881 agaccaccca cgcccaggat ttccccctgg tgcaacttga aggagatgcc gtggacgccg
     2941 ggcgcggtaa gctcttttgc ctccaggaca accttgctcg actgccgcgg ggcaagctct
     3001 ggatacatcg catccacctt gcggcccgtc atcagggtga tcccctgatc ttcgtcaagt
     3061 cccgtatatg tgcccacgtg cacgccgttg cggaagatgg tgatccggtc ggcgatgctc
     3121 tcgatctcct tccagcggtg agaggtgaag atgatgcact tacccctttg ccgaagcccc
     3181 tttatcaggt cgaagagcca gatcacctcc cgctccgcga gcgaggaggt cggctcatcc
     3241 atgaacagga tgtcaggatc tcggatgacc gcatgagcga tctcgacgat ctgctgttgc
     3301 gccagagaca ggttggctac cagttccatg ggatctatct gctcgatccc cagaccggcc
     3361 aggatctctt cggcgtgatg gcccatctgc ccacgccgga tcagtcccag gccgttccgc
     3421 ggctcgtggc ggagaagcag gttctccgcc acggtcatcc acggcatgag ggtgagctct
     3481 tgaaagaccg tcccgatccc cagcgactga gccgcctgtg ggctgctcaa ctcgacctgc
     3541 tggcctcgaa cccgaatgac acccgagtca ggatggatca acccgctcag gatcttgatc
     3601 atggtgctct tgccggcacc gttctccccg accagggcgt gaacctcgcc gaagtcggcc
     3661 tggaagctgg cgccgtccag agcttgaacc gcaccaaagg ctttgaagac gttcagtcct
     3721 tcgatcgccg ggatggcttt atcgaaagcg atcactcgag tctcaccttc atcagttctg
     3781 gccactgcag ctacggcttc ggcaggttga ccttcaggct accgtcgcag gcggtgccgc
     3841 tcaggccggc atccacgcat agctgcacgg tggcgttgtc gccgctatcg gtgaagtcgg
     3901 tgaagaagct gtcctccaac tcagggaaca ccgtctcgcc cgacttaacc gtgtcggtgg
     3961 tcacgtaggg gaacgggatc tcgatatcct tcttgggata gcggttctgg aggattgctc
     4021 gcgcgagttc cagcgacacc acgctcaaga agggaggctg gccgatggac atggcggtga
     4081 tcttgggctg ccccatgtag tcgcccagca tgaacttgcg gaagccgttc tcggcctcgc
     4141 cggccacgac gggtaccggc ctgccggcag cgatgaacgc cttaatcaca ccgtctgtac
     4201 cgccctggca ccagatgccg tcgatcttgg gaagtgaggg caggacaccg gcggcattgc
     4261 gctcggcaac cgaggagtcc cacataccgg taaagcggtt gacgaccttg atatccggat
     4321 acttggccca gacggagtcg gccccagcgt tgcgctgctc atctacggtg gtaccggcca
     4381 caccggtgac catgatcacg ttgcccttgc cgcccatctt gtccgcgagg aactgggcga
     4441 gctgggcgcc gaacttgaac tggtcggtgt tgacctttag cgcggcggga gtggtcacgg
     4501 tattatcgaa ggagacgacc aggatccctc gctgcgcggc ctgttcgacg atgccgttga
     4561 gacccgtcgg ggaggctgca ttgaggacga tggcatccac cttctgggag atgaggttgg
     4621 aaagctgctg ggattgcttg ctgacgtcgt tgcccgagct gtagatggaa ccatcgacct
     4681 cagacttgta aggctccata tccagggcag cgcggaagac gttggccatc tcgatacgcc
     4741 acttgttccc gatgtacgag ttggccaggg caatcttcag cttcttgccg gcggcaggct
     4801 gtgcctgggc ggctggggca gtggtggccg caactgccgg cggggcggcc gctgtggcgg
     4861 gagccaccgc ttgagatgca gccgccgtgg taggtgcagc cggtgccgtt ggggcagggc
     4921 tggattggca tcctgccacc gcaaccagca tggcgatggc cgtgagtggg ataagggaac
     4981 gtcgtagaag ggtccgaata tccccgtacc gtcgggtgta agcttttgcc ataggagttc
     5041 tcctttattg ggcgcacgcc cgatagttgc gccttggatg aagtcccggt gtcttgtgcc
     5101 ccgaccgcga gggaatgcgc gagttcgggg ctcacacaaa tcgaggcctc agccttcgat
     5161 gggcggaagc ctccatgggt tcagaaccgc agaacaaaag gagctaccgg tctttctcta
     5221 cccgcttgta ggtctcacct cccttgctcg aaatcgcagt tttcgtcctt tgcagatcat
     5281 cttagccaca atatcttcct tgttcgtatt tgcagctaat tcttgcacaa tgatcatacg
     5341 catactttgt accatgttga agatagtcgt tattacagat agttatcgtg tttaccacta
     5401 ccatgggcgg catcggagtc gcccccatgg cggcccccat tcgcgttaca atgggatcaa
     5461 caacctacat gggaggtact gcccgtggac gtccagcaaa tcatcgccgc cctgaatctc
     5521 cagccccacc caaaggaggg cggctacttt gtggaaagct tccggtccga agggagactc
     5581 ctggcagaag tcctcccgga gggcttctcc accccccgcg ctctctcgac ggccatctac
     5641 tacctgctga ccccggccac ctactcggag atgcaccgac tcccgggcga cgaaatcttc
     5701 cacttctaca tgggtgaccc cgtagagatg ctgcagttgt ggccggacgg gaccggaaag
     5761 gtggtagagc tcgggacgga tctggagtct gggcagaggc cccaggtggt ggtgccgggt
     5821 gaggtgtggc aggggagcag gctcaagcag ggcggcagct acgcactgct gggcacgacc
     5881 atgtcgccgg gcttcgacta tgaggactac gagagcgggc aaaggacgaa aactgcgatt
     5941 tcgagcaagg gaggtgagac ctacaagcgg gtagagaaa
//
LOCUS       DATALS010000201         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_324636, whole genome shotgun sequence.
ACCESSION   DATALS010000201 DATALS010000000
VERSION     DATALS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324636"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2654
                     /locus_tag="VHS06_04515"
     CDS             <1..2654
                     /locus_tag="VHS06_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HEX2987416.1"
                     /translation="MMHGPFQFHGNALFYLIFGASDYTARMMAATFGVWIVFSPFFLR
                     REIGRAGALVASFLFAVSPSFLYFSRFTREDIYIAGFEMAIVIAIFGLLRTGKSRYAY
                     LLFAGTALAYATKESVFITGFIFLIFFAIEGIVAGTRKQDPALLRLLRSISARDWAIC
                     IAIFLTIYVLLYTTFFTNPKGLLSGSVGALTYWLAQQGVQRGGQPWFYYLLLIPLYEF
                     LPLLAALGGLVFLAVRRRLSSVGFFGGFMGVWVLGALVVYSWAGEKMPWLDIHITQPL
                     VFLAAMAIAGLLKRINRGLFTNRGGLVALVMTCVLAAVLLGGLVAAPPVGGTQLGMQS
                     ATLQRVAMWLVAGALFLALMAMARSRGSEAVLVPAATGFLVALLVLSIHTAWGVTYER
                     GDTPQDMLVYVQSSPDVTRTMKQVEDIAARTGSGKDMRILLDGGYTDTVGGQSVSHES
                     IAWPFEWYLRDWKGKSYYSRTLPNPTDAPIILAMTANEDPIRSQLSNYVAVRGRLNWW
                     YPEDYKGLTWSRVWDGLTDPSVRAKLWRYFLYKETLNPLGSRDFDLFVRSDLARGIPL
                     QPVSASAPAAQVTAAGAPPSEAVAQAAPGGITVFGKTASGTSVLVDPKGAALGSDGLL
                     YVVDSAACKVYGFRPDGTVAVQFGKKGNGDGEFNEPWGIAVARTGEIYVADTWNHRVQ
                     KFDRNGKLLAKWGSFADTKGQLNDQQGSFWGPRDIAVAPNGNVLVTDTGNKRVEVFDS
                     QGKFLSLFGGDGTAPGNLKEPVGITVDTSGNIYVADTWNQRIQKFDAQQRPIAQFAVP
                     GWNSQSIANKPYLAVDQDGRIYYTEPEKNRFVVLSPNGQQEGAKGVQGADPTSFNLPI
                     GIDVSPQGEVYVVDSRNARAVRYQSWR"
     gene            2656..>2907
                     /locus_tag="VHS06_04520"
     CDS             2656..>2907
                     /locus_tag="VHS06_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX2987417.1"
                     /translation="MSLRGRLGRLLGFLLTAFFLWIVARNVSLDELVNAFRGADYRMV
                     VPATLITLTGYILRTVRWQRIIKPVAALGFRDAFSVLMMG"
BASE COUNT          525 a          871 c          893 g          618 t
ORIGIN      
        1 caatgatgca cggtcccttc cagtttcacg ggaacgcgtt gttctacctg atcttcggcg
       61 caagcgatta caccgccagg atgatggccg ccacattcgg tgtgtggatt gtcttctcgc
      121 ccttcttcct gcgccgtgag atcggccgtg caggcgccct cgtcgcctca ttcctgttcg
      181 ctgtgtcgcc ctctttcctc tacttctccc gcttcacgcg agaggacatc tacatcgccg
      241 gcttcgagat ggccatcgtg atcgccatct tcggcctgct gcggaccgga aagagtcgct
      301 acgcctacct gctgttcgcg ggaaccgcgc tggcatacgc caccaaagag agcgtcttca
      361 tcacaggctt catcttcctt atcttcttcg ccatcgaggg gatcgtcgcc ggcacgcgca
      421 agcaagaccc cgccctgttg cgcctccttc ggagcatttc cgcgcgcgac tgggccatct
      481 gtatagccat cttccttacc atctacgttc tgctctatac gacgtttttc accaacccca
      541 agggtctcct gtccggtagc gtgggggccc tgacctactg gctggctcag cagggtgttc
      601 agcgaggcgg gcagccgtgg ttctactact tgctgctcat ccctctgtac gagttcctgc
      661 ctttactggc cgcgctcggt gggttggtgt tcctggcagt tcgtcgacga ctgagcagcg
      721 tgggcttctt cggcggcttc atgggcgttt gggtgctcgg cgctctggtc gtgtactcgt
      781 gggccggaga gaagatgccc tggctcgata ttcacataac ccaaccgctg gtcttcctgg
      841 ccgcgatggc catcgctgga ctgctcaaga ggatcaacag ggggttgttc accaatcgcg
      901 gcggtctcgt tgcgctggtt atgacctgcg tcctggccgc ggtgctgttg ggtggacttg
      961 tggccgcgcc accggtggga ggaactcaac tcgggatgca gtctgccaca ctccagcgag
     1021 tggccatgtg gttagtcgcc ggagcgctct tcctcgccct tatggcgatg gcgagaagtc
     1081 gagggtctga agcggtcctt gtgccggcgg caacagggtt cctggtggca ctgctggtac
     1141 tcagcatcca caccgcatgg ggcgttactt acgagcgtgg cgacacccct caagatatgc
     1201 tcgtgtacgt ccagtcctcg cccgacgtca cgcggacgat gaagcaggtc gaggacatag
     1261 ccgccaggac gggatcgggg aaggatatgc gcatcctgct cgacggcggc tacaccgata
     1321 cggtgggcgg acagtcggtt agccacgaga gcatcgcctg gcccttcgag tggtaccttc
     1381 gcgattggaa gggcaagtcc tattactcga ggacgctgcc aaacccaacc gacgcgccta
     1441 tcatcctggc aatgaccgca aacgaggacc ccatacggag tcagctttcc aactatgtgg
     1501 ccgtacgcgg gcgtttgaac tggtggtacc ccgaggacta caagggactc acgtggagta
     1561 gagtgtggga cggtcttacc gacccctccg ttcgggccaa gctatggcgc tacttcctgt
     1621 acaaggaaac gttgaacccg ctggggtcga gggatttcga tcttttcgtt cggtccgatc
     1681 tggccagagg gatccctctc caacccgtct cggcgtcggc accagccgcc caggtcacgg
     1741 ctgcaggcgc ccctccatct gaggcagtcg ctcaggccgc gccgggtggc ataacagtct
     1801 tcggaaagac cgcctccggc accagcgtgc tggtcgaccc gaagggcgcc gcactggggt
     1861 ctgacggtct gttgtatgtg gtggattcgg ccgcgtgcaa ggtctatggc ttcaggcccg
     1921 acggcaccgt cgcagtccag ttcggcaaga aggggaacgg ggacggggag ttcaacgaac
     1981 cgtgggggat agcggtagcc cggacggggg agatttacgt cgccgacacc tggaatcatc
     2041 gcgtgcagaa gttcgatagg aacgggaaac tgctggccaa gtggggcagc ttcgccgata
     2101 ccaagggaca actgaacgac cagcagggct ctttctgggg tccccgggat atcgccgttg
     2161 ccccgaacgg aaacgtgctg gtgaccgata ccggcaacaa gcgtgtcgag gtcttcgact
     2221 cgcagggcaa gttcctgagc ctattcggtg gagatggaac tgcgccgggt aacttgaagg
     2281 aacctgtagg cattacggtg gacaccagcg gcaacatcta cgtcgccgat acttggaacc
     2341 agcgcatcca gaagttcgac gcacagcagc gtccaatcgc gcagttcgca gtcccgggtt
     2401 ggaactctca gtctatagcc aataagccgt acctcgccgt cgaccaggat ggtcgtatct
     2461 actataccga accggagaag aaccgcttcg tcgtcctgag ccccaacggg caacaggaag
     2521 gagcgaaggg agtccagggc gcagacccta cctccttcaa cctcccaatc ggtatcgacg
     2581 tctcgcccca gggcgaggtc tacgtcgtgg atagccggaa cgcccgggcg gtaaggtatc
     2641 agtcctggag gtaagttgtc tctacggggt cgtcttggcc gtctgttggg attcctgctg
     2701 acggccttct ttctgtggat agtcgccagg aatgtcagcc tggatgaatt ggtcaatgca
     2761 ttcaggggtg cggactaccg catggtggtc cctgcaacgc tgattaccct cactgggtac
     2821 atcctgcgga cggtgcgctg gcagcgaata atcaaacctg tggccgctct cgggtttagg
     2881 gatgcatttt ccgtcctcat gatgggc
//
LOCUS       DATALS010000202         9054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_245432, whole genome shotgun sequence.
ACCESSION   DATALS010000202 DATALS010000000
VERSION     DATALS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9054
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245432"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2363)
                     /locus_tag="VHS06_04525"
     CDS             complement(<1..2363)
                     /locus_tag="VHS06_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2987418.1"
                     /translation="MDENEQQRARRTRRRPEADRLRDAMVAVALSLTQELEWDRVVKR
                     VLRTARDILRAEASMLWCDVARRGTVQLMAYEGVSEDDLAPLREIHLREPLLPSRGAR
                     KVSPVFSSPPADLPEEYSPFQGLERLGFSRLTILPLLARGHLEGVLTCLRHTPLDYGD
                     AEKDAISTIAGIMAVGLKNARQYADLKRSGEAVSRLAAIVESSEDAIIGKTLDGTIVS
                     WNPAAEDLYGYRSDEMIGKPISVIFPPERADEVQFILNRVRRGKRVTHYETVRQRKDG
                     SRLDVLIAVSPVRDSSGKIVGAATTAHDITARKRADRALRASEERFRAIFEQAAVGMA
                     IVSLDQRLLRVNQRLSDIVGYSPEQLVSMSVRDITLPEDRPKDDEYFGRLRRGELHTY
                     VREKRYVRRDAAVVWVGLAVSLVHTSDSGEPDSVVVVVEDINDRKTAQQERERLLAEL
                     DATISSIPDGILVYGPKGETVRMNPAAIRMLGGPQQRWEIPTEERLRLLRIETPDGTK
                     LSLEDFPSVRALRGETVQGLILVIHPPGLKPVWVSAAVAPIQGPSGECIGGVVAFTDI
                     TELHQLQEQREDLLRMVSHDLRSPLTAVQGQAQLLLRLMDRSGQDGPMRQGMEGILSS
                     ARRMNSMIQDLVDLARAESQQLQLKREPVQLRALVLDMKQRLAGALDPSRIRVEIPDD
                     LPAILADPGRLEQVLTNLIGNALKYSPPSTPVVVKAEASRSETTVSVTDMGRGIAAEE
                     VPHVFDRYYRTRSARGVEGLGLGLYITKMLVEAMGGRIWVDSEVGRGS"
     gene            2628..2804
                     /locus_tag="VHS06_04530"
     CDS             2628..2804
                     /locus_tag="VHS06_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987419.1"
                     /translation="MMRFGMMRMRPPGFPWFPFLFPMGLFMLMMGITTWFTYQSWHEL
                     DEIRRTGVSGSHRG"
     gene            complement(2893..3612)
                     /locus_tag="VHS06_04535"
     CDS             complement(2893..3612)
                     /locus_tag="VHS06_04535"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX2987420.1"
                     /translation="MRRTALKTALGVVTVMCIVGTASAQTFPQGRAGGQDPQGGPVAG
                     TIDSISDNSMVVTTRDGSTVTVDVTDAKVRSIEVGSSSDLASDQMVTVVGQKNSDGTV
                     TATTVEIEPADAPGEGQRPIDGQGQPPMGGQGQPPMGDQGQRPMGGQGQPPTDGQGQP
                     QGAGSRPIVMGTVVSLDGNTLTIKDMQGNSIQVDISSAEIRKTVDSSVSSLAAGQSVM
                     AMGQKNSDGTVTAINVVIRPS"
     gene            complement(3876..5102)
                     /locus_tag="VHS06_04540"
     CDS             complement(3876..5102)
                     /locus_tag="VHS06_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2987421.1"
                     /translation="MPDMTVDQARQFLEGFDHCAHPLSMRYLNMALDRIIVTMGLVPR
                     LQGNVRVLELGAMPYLMTALMMRRFPNYSMELANEPDRLKEDGGRARLIHRGEGIDLT
                     LEYKGFNLETDRFPYEDGSFDIVLYCEIIEHMMYDPTHSLYEIHRVLKPGGHMLITTP
                     NAFRYAHYVQLLTGENIYPPYSGYGPYARHHREFSPKELRLLLKECNFEMEELITAYD
                     PAYHDGKSRLDGLARWLYGLGFLREKMDVIHLRARAVGKPVFRYPPELYLDVHAYHPA
                     VDDSLEMGAKEDGRLESGFHAVESWPPTVRWTDRHARIRMESHGQAVLGIHFYSGPKE
                     LARQVEGNISLNGVAHRFSVAAGEWAELRFPIPAGTTGPLLVEIDLDQTWVPRDLKLD
                     SDSRQLGVALQRLWLE"
     gene            5349..6419
                     /locus_tag="VHS06_04545"
     CDS             5349..6419
                     /locus_tag="VHS06_04545"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX2987422.1"
                     /translation="MSQILTERIRALLVEETDAARMKGVVVAAALPGEEAEYLALGTD
                     ASGRELTTDTLFSVASITKLATSLAVLRLVDRGRISVDDPLSRHLPEADAAQEGVTVR
                     RLLSHSAGMPIDPPSELAPYEPGLDWSVLARACKHTPPERAPYGAVQYSNVGYGLLAL
                     IVEKETGLDFPEALRDLVLRPLGIEGYLGEEPPRLPAQLTGVRGRHVGTPLEPFNSPF
                     WRSLGMPWAGLVTTVAGALTLVRAFQGVPAGFLRAETVAEATRNQNDDLSGGYVQPLF
                     WKRCPWGLGPEIRGEKSPHWAPPEAGPTSFGHAGASGCLAWADPATGLSWAIMGSRTS
                     DSGWLLRRGTVMSRAFLEAARG"
     gene            complement(6469..7458)
                     /locus_tag="VHS06_04550"
     CDS             complement(6469..7458)
                     /locus_tag="VHS06_04550"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX2987423.1"
                     /translation="MRPNGTSPSTLHNDSIIVDTHCDALGTVVEGRRRLGERSSDGQL
                     DLPRALEGGLTAEFLAIYLDAPQPGNNLRKAIQYVDLFYREIESYPDQAIHVTRADDI
                     QLAKKQGKVGLLLSMEGAEPLEGDLGALRTFYRLGLRSLGITWNRRNEAADGVGELGT
                     GGGLSHFGEELVKECNRLGIMLDLSHLAPAGVEDVLELSETPPVATHANCHTLWQHPR
                     NLTDSQLEAIAAKDGVVGVAAVPLFLGEDRNRSSLSVMLDHLDHMVGVMGEDHVGLGT
                     DFDGVGDARVEGLDDASKLPNLTAALLDRSYSDEAISKFLGGNFFRVLRQVIG"
     gene            complement(7462..8067)
                     /locus_tag="VHS06_04555"
     CDS             complement(7462..8067)
                     /locus_tag="VHS06_04555"
                     /inference="COORDINATES: protein motif:HMM:NF020509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX2987424.1"
                     /translation="MARRVYFASYYEDVSDFRLRTVLSAWVAGSFRETSGIFNAAQWE
                     KAKLQGDAAVRAMIRRGMEGTSVTVVLIGTNSYSRRYTRYEIAKSFERGNAMIGIYVH
                     RIPEQGTIAIKGENPFEYMGFSLDPSRSKIGLLEKRVGTGKWLEYAELPSVHFGLAPY
                     NFLRLKEGAFSSLFSVHDWMDGQGMRNLAPWIETAAKQAGK"
     gene            complement(8369..>9054)
                     /locus_tag="VHS06_04560"
     CDS             complement(8369..>9054)
                     /locus_tag="VHS06_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2987425.1"
                     /translation="ADRRSIEGRNYLELVRSARASGHDLVAIGAHGLGRVERSVLGSV
                     CERVARLADCDLLVAREDRPIGDGPLLVALDGSDQAFAAMGVALQLARLFDNEVMAVA
                     AYDPFFHGGAFQSIATVLSPEASRLFRFREQERLHDEIIDDGLSTIYRSHLDQAERMA
                     ASAGVQLLCEVIEGKPFDAVAGYALKLHPSLLLAGRTGIHHCDGVTLGSTSENLLRCV
                     QCNVMIVSR"
BASE COUNT         1703 a         2944 c         2677 g         1730 t
ORIGIN      
        1 gagccccgcc ccacctcact atccacccag atacggcctc ccatcgcctc cacgagcatt
       61 ttggttatgt aaaggcccag ccccagaccc tccacgcccc gggcactccg cgtcctgtag
      121 tacctgtcga agacgtgcgg aacttcctcg gcggcaatac cgcgtcccat atccgtcacc
      181 gagacggttg tctccgagcg tgaagcctcc gccttcacca ccacgggggt tgaaggcggc
      241 gaatacttca gcgcgttccc gatcaggttg gtgagaacct gctccagccg gcccgggtcg
      301 gccaggatgg ccggcagatc gtccgggatc tccacccgaa tgcggctggg atccagcgcg
      361 cccgccagcc tctgcttcat gtccaggacg agggctcgca gttgaacggg ttcacgcttc
      421 aactgcagct gctgcgactc cgcacgggcc agatccacga ggtcctggat catcgagttc
      481 atcctcctgg cgctggagag tatgccctcc atgccttgcc gcatcgggcc gtcctgtccc
      541 gatcggtcca tcaaccgcag caacagctga gcctgcccct ggaccgcggt cagcgggctt
      601 ctcagatcgt gtgacaccat ccggagcagg tcctctcgct gttcctgaag ctggtgaagc
      661 tcggtaatat ccgtgaacgc cacgacgccg ccaatgcact ctcccgacgg cccctggata
      721 ggggcaaccg cggccgagac ccacacaggt ttgagacccg gcgggtggat gacgaggata
      781 agcccctgca cggtctcgcc cctcaacgca cggaccgagg gaaaatcctc cagcgacaac
      841 ttagtgccat ccggggtttc gattcgcagc agccttagcc gctcttctgt tgggatctcc
      901 caacgctgct gcgggccgcc aagcatccgg atcgccgcag ggttcatcct taccgtctcc
      961 cccttcggcc cgtagacgag gatcccgtcc ggtatggacg atatggtggc atccagttcc
     1021 gccagcagcc gctcccgctc ctgctgggcc gtcttgcgat cgtttatgtc ctcgacaacg
     1081 acgacgacgc tgtccggctc tccgctatcc gacgtgtgca ccagcgacac agccaggcct
     1141 acccaaacca cagcagcatc gcgacgaacg tagcgcttct cccgaacgta ggtgtggagc
     1201 tcgcccctcc gcagccgccc gaagtactca tcgtctttgg gtcggtcctc cgggagggtg
     1261 atgtcccgga cagacatgga cacaagctgc tcggggctgt aacccacgat gtcgctgagt
     1321 ctctgattga cccgaagcag ccgttggtcc aggctcacga tcgccatgcc gacggcagcc
     1381 tgctcgaaga tagccctgaa tcgctcttcg ctcgcccgca gcgccctatc ggctcgtttg
     1441 cgagcggtaa tgtcgtgcgc ggtggtggcc gcgcccacta tcttcccgga ggaatcccgt
     1501 accggagaga cggcgatcag gacgtccagc ctggagccat cctttcgctg ccgcaccgtc
     1561 tcgtagtggg tcactcgttt cccccgcctc accctgttca ggataaactg cacctcgtcg
     1621 gctcgctccg gcggaaagat gacggatatc ggcttcccga tcatctcatc cgaccggtag
     1681 ccgtacagat cctcggccgc agggttccag ctgactatcg tgccatccag agtcttgccg
     1741 ataatggcgt cttctgagga ttccacgata gccgccagac gggataccgc ctcacccgat
     1801 cgcttcaagt cggcatactg tcttgcgttc ttcagtccca cggccatgat gcctgcaatg
     1861 gtggaaatgg catccttctc agcgtcgccg taatcgagtg gcgtgtgcct gaggcaggtc
     1921 aggacccctt cgaggtgtcc cctcgccagc aacgggagga tagtaagccg gctgaatccc
     1981 agtctctcca acccttggaa tggcgaatac tcctcgggta gatcggcggg ggggctagag
     2041 aagacgggag atactttacg cgctcctctt gagggaagaa ggggttcgcg caggtggatc
     2101 tctcgtaggg gggccaggtc gtcctctgaa acgccctcgt aagccatcag ctgcactgtt
     2161 ccccgccggg ctacgtcgca ccatagcatc gatgcctcgg cccgcaagat gtctctcgcc
     2221 gtccgaagca ctctcttgac gacacgatcc cactccaact cctgtgtcag cgaaagtgca
     2281 acggccacca ttgcatccct cagccgatcg gcttcgggcc gcctcctcgt tcgtctagcg
     2341 cgctgctgct cgttctcgtc catgccgctc tcgttggtcc aggtgagacc gtgaccggcc
     2401 caccccacgt tggatgcaaa ggtagcatga tggtcagcat ggaccgtacc caggaggccg
     2461 gacctctacc ttctctactc ggtatcgatc ttgccatcac ccgcccccac gactgcggag
     2521 atgggccagg ccctggcagc catctctcca gcacgggttc tcagaccgcc gcacggcggt
     2581 ggctgaggaa gcgaagccga cttcagttta gacagtgagg ttgccatatg atgcggttcg
     2641 gaatgatgcg aatgcgccca cccggctttc cctggtttcc gttcctgttc ccgatgggcc
     2701 tcttcatgct aatgatgggg atcaccacct ggttcaccta ccagagctgg cacgaactgg
     2761 atgagatcag acgcaccggc gtgtccgggt cacacagagg ctgacgggac ggcacgacca
     2821 gacgaagaca gccacagatg tgggctgagg acagggccac ccgttcaacc gggtggccct
     2881 gcttcaagag gatcaggatg gccggatcac gacgttgatc gcggtcacgg tgccatcgga
     2941 gttcttctgg cccatggcca tcaccgactg tccggcggcg aggctgctga ccgacgagtc
     3001 cacggtcttt cgaatctcgg cagacgagat gtcgacctgg atagaattgc cctgcatgtc
     3061 cttgatagtc agggtgttac cgtccagcga taccactgtc cccatcacga tcggtcgaga
     3121 ccccgcacct tgcggctgcc cctggccatc agtcggcggt tgtccctggc cgcccatcgg
     3181 ccgctgtccc tgatcaccca tcggcggttg tccctggccg cccatcggcg gttgtccctg
     3241 gccgtcgatg ggccgctggc cctcgccggg tgcgtctgcg ggctcgatct caacggtggt
     3301 tgccgtgaca gtaccgtcgg agttcttctg accgacaacc gtcaccatct ggtccgacgc
     3361 caggtcggaa ctactcccaa cctcgatgct ccgtaccttg gcgtcggtga cgtcgacggt
     3421 gacggtgctg ccgtcgcggg tggtcaccac catgctgtta tcgctgatcg agtctatcgt
     3481 gcccgcgaca gggccacctt gcggatcctg gcctccagca cggccctgcg gaaaggtctg
     3541 cgcactggca gtgcccacga tacacatgac tgtgaccact cctaaggcgg tcttgagagc
     3601 cgttcttctc atgaaaaacc tccacattgc ttgctggctc cctggaggat gagcgccaag
     3661 cgagagggcc acgtggcgtc ctctcggccg gtagccgtca ctcttttggc gactaccgga
     3721 tcgaagaggt tccctctcac ctgggttgcg atcctctgtc caggtgcatg aggaggataa
     3781 tggaagcagg gtaaagatgg agtttaggcg gtgttaggat ggcgttaaga tgatcatccc
     3841 gccggccccc aggggttgag cgcccctgga ggtccctact cgagccagag acgctgaagc
     3901 gcgaccccca actgccggct gtcactgtcc agcttcaggt cgcgcggcac ccacgtctgg
     3961 tccaggtcga tttcgaccag caatggaccg gtcgtaccgg caggaatggg gaagcggagt
     4021 tccgcccact cgccggccgc gacagaaaat cggtgagcta ccccgttcaa cgagatgttg
     4081 ccctcgacct gcctcgccaa ctccttcggg ccggagtaga agtggatgcc caggacggcc
     4141 tgtccgtgtg actccatgcg gatccgagcg tggcgatcgg tccagcgcac cgtcggcggc
     4201 cagctttcaa cggcatggaa gccggattcc agccgtccgt cctccttagc ccccatctcc
     4261 aggctatcgt ccacggcggg gtggtaggca tgaacgtcca ggtacagttc gggtgggtag
     4321 cggaatacgg gcttccctac ggcccgcgct cgcagatgaa tgacatccat cttctcacgc
     4381 aggaacccca gaccgtatag ccatcgagcc aagccgtcca gacggctctt gccgtcgtga
     4441 taagccgggt cgtaggccgt tatcaactcc tccatctcga agttgcactc tttcagcagc
     4501 agccgcaact cctttgggct gaactcgcga tggtggcgag cataggggcc gtagcccgag
     4561 tagggcgggt agatattctc acctgtcagg agctgcacgt agtgagcgta gcgaaaagcg
     4621 ttgggggtgg tgatcagcat gtggccgcct ggcttgagca cccggtggat ttcgtagagc
     4681 gagtgggtgg ggtcgtacat catgtgctcg atgatctcgc agtagagcac gatgtcgaaa
     4741 ctgccatcct cgtagggaaa gcgatctgtc tcgagattga accccttgta ctccagggtg
     4801 aggtcgatcc cctcccctcg atggatcagc cgagcgcgac cgccgtcttc cttgaggcgg
     4861 tccggctcgt tggccagttc catgctgtag ttgggaaacc ggcgcatcat cagggcggtc
     4921 attaagtagg gcatcgcccc aagttccagc acccgtacgt tgccctgaag cctgggcacc
     4981 aggcccatcg tcacgattat ccggtccagg gccatgttca ggtagcgcat ggacagggga
     5041 tgggcgcagt ggtcgaatcc ctcgagaaac tggcgggcct ggtcaactgt catgtcaggc
     5101 atgaagatgg cgtattccaa tcatgttgaa tatccgtcgc ggatgcagtc gcatccaccg
     5161 gcctccagat tgaggcagcg acgcaccgcg tagggtagca cagagccgcg ggcaaccgcc
     5221 acgcaccggt ggcagccaac ttgggatagg gtgtatcgta ggcaacgcga atggagtcga
     5281 ggcgccggcc ggaacagaca gcaccgatct gagcctctcg ctccaccttc tggacggagg
     5341 taggttaggt gtcacagatc ctgacggaga gaatcagggc gctgctggtg gaggagaccg
     5401 acgcagccag aatgaagggg gtagtggtag ccgcggcgct accgggagag gaggcagagt
     5461 acctggcgct cggcactgac gcgtccggcc gagaactcac aaccgacacc ctcttctcgg
     5521 tggcctccat caccaagctg gcaacgtcgc tggcagtgct cagactggtc gaccgggggc
     5581 gaatctccgt ggacgatcct ctctcccgac atctcccgga agccgatgca gcccaagagg
     5641 gagtcacagt gaggaggctc ctaagccact ccgccgggat gccgatagat cctccctcgg
     5701 agttggcccc atatgagccc ggcctggatt ggtccgtcct ggctcgcgcc tgcaagcaca
     5761 cgccgccaga aagggctccc tacggcgccg tgcagtacag caacgtcggc tacgggctgc
     5821 tggctttgat cgtggagaag gagaccggcc tcgactttcc cgaagcgctc cgcgacctgg
     5881 tgctgcgacc gctggggatc gagggctacc tcggagagga gccgccccgc ctacctgcgc
     5941 agctcacagg cgtcagggga cgtcacgtcg gcacacccct ggagcccttc aactccccct
     6001 tctggcgcag cctgggaatg ccctgggcgg ggctggttac cacggtcgca ggggcgctga
     6061 cactggttcg cgcattccag ggggttccgg ccggatttct acgagcggag acggtggccg
     6121 aggcaacccg aaaccagaac gatgacctgt cgggcggtta cgtccagccc ctcttctgga
     6181 agcgctgtcc atggggactg ggccctgaga tccgtgggga gaagtcgccg cactgggcgc
     6241 caccggaggc cggcccgaca tccttcggcc atgccggcgc gtcgggatgc ctcgcctggg
     6301 cggacccggc gacaggacta tcctgggcaa tcatggggag ccgtaccagc gatagcggat
     6361 ggctgctccg ccgaggcacc gtcatgtcga gggcgttcct ggaagcggcc agggggtaac
     6421 acccacctgg agtcggggtt gtctcaaccc cgactcccag aatcaggatc agccgatcac
     6481 ctgtcggagc accctgaaga aattgccacc caggaacttg gagatggcct cgtcggagta
     6541 acttcggtcg agcagggcgg cagtcaggtt gggcaacttg ctcgcgtcgt ctaacccttc
     6601 gactctcgcg tcgcccactc catcgaagtc ggtgccgagc cccacatggt cctcgcccat
     6661 cacccctacc atatggtcca ggtgatccag cattacgctg agcgaagagc gattgcggtc
     6721 ctcaccgagg aagaggggga ctgccgcaac cccgacgaca ccgtccttcg cggcgatcgc
     6781 ctccaactgt gagtctgtca ggttccgagg atgctgccag agggtgtggc aattggcatg
     6841 agtcgcaacc ggtggggtct cggacagttc aagcacatcc tccacccctg caggggctag
     6901 atgcgacaga tccagcataa tccccagtcg attgcactcc ttcaccaact cctcgccgaa
     6961 gtggctcagc ccaccccccg tgcccaactc cccaacgcca tccgcggcct cgttgcgccg
     7021 gttccatgtg atccccaggc tgcgaagccc cagccgataa aacgtcctta gcgcccccag
     7081 atctccttcg aggggttccg ccccctccat gctgagcaaa aggcccactt tgccctgctt
     7141 ctttgccagt tggatgtcgt cggctcgagt aacatggata gcttgatccg gataggattc
     7201 gatttcgcga tagaaaaggt ccacgtactg gatcgcctta cgcaggttgt tgccgggttg
     7261 aggggcatcg aggtagatgg ccaggaactc tgccgtgagc ccgccttcta gcgcccgtgg
     7321 tagatctaac tgcccatcgg aggagcgctc tccaagcctc cgccggccct ccaccacggt
     7381 gcctagagca tcgcagtgag tgtcgacgat gatgctgtca ttatgaagcg tgctggggga
     7441 tgtaccgttc ggtctcatcg gctactttcc cgcctgcttg gctgccgtct cgatccaggg
     7501 cgcaaggttg cgcataccct gcccatccat ccagtcgtgg acggagaaca aactggagaa
     7561 tgcgccctct ttcaatctaa ggaaattgta gggcgcgaga ccgaagtgga cgctggggag
     7621 ttcggcatac tccaaccact tgccggtgcc gaccctcttc tccagaagac cgatcttgct
     7681 cctggatgga tcgaggctga agcccatata ctcgaaggga ttctcccctt tgatggcgat
     7741 cgtcccttgc tcagggatcc ggtgaacgta gatcccgatc atcgcattgc cgcgctcgaa
     7801 gctcttggcg atttcgtagc gggtgtatcg ccgggagtag ctgtttgtgc cgatcagcac
     7861 caccgttacg gaggtaccct ccatccctcg cctgatcatt gccctgacag cagcatcacc
     7921 ctgcagcttt gccttctccc attgcgccgc attgaagata ccagatgtct cccgaaaact
     7981 gccggctacc catgcggaaa gcacggtcct gagacggaaa tcggagacat cctcgtagta
     8041 cgaggcgaaa tacacccgcc tggccaatac tacaccctcc taagaacgtc tctgcccatc
     8101 atcctcgccg gtcaattagg tgaccccaag tcgccgcgtg aatgcagagg ggattcgata
     8161 acgccatgga atcgccaagg caacggcgaa ggcagggaga caaaaccggg agtccaaaca
     8221 ctcatagcat cgggcaaccg cgcgtcccag cgggttgatt gtaccatata cttcgatgaa
     8281 gaactgttag ggctgggact cccgcgagag tctcaacctc aatggacatc agaataccga
     8341 aggattagcc cgtgcggccc tcgcgggact acctactcac aatcatgacg ttgcactgta
     8401 cgcaacgcag caggttctcc gaagtgctcc caagggtgac gccatcacag tgatgtattc
     8461 cggtccgccc cgccaggaga agagagggat gcagcttcag ggcatacccc gctactgcgt
     8521 cgaaaggctt gccctcgatg acctcacaca gcaactgtac gccggcgctg gccgccatcc
     8581 gctccgcttg gtccaagtgc gaccggtaga ttgtggagag gccgtcgtcg atgatctcgt
     8641 cgtgcagcct ctcctgctcg cggaatcgga atagccgcga agcttccggc gacaggaccg
     8701 tggcgatgct ctggaaggcg ccaccgtgga agaatggatc atacgcggca acggccatca
     8761 cctcgttgtc gaataatcga gccaactgaa gggcaacacc catagcagca aatgcttgat
     8821 cgctgccgtc gagggctacc agcagcggac catctcctat cggccggtcc tctcgcgcca
     8881 ccagaaggtc acaatccgcc agcctggcta cgcgctcgca cacgctgccc agcacggagc
     8941 gctcgacccg ccccaatcca tgtgcaccga tggccacgag gtcgtggccg ctcgcccggg
     9001 cgctccggac caactccagg tagttgcgcc cctcgatgct ccttcggtct gcct
//
LOCUS       DATALS010000203         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_155184, whole genome shotgun sequence.
ACCESSION   DATALS010000203 DATALS010000000
VERSION     DATALS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155184"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VHS06_04565"
     CDS             complement(<1..239)
                     /locus_tag="VHS06_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987426.1"
                     /translation="MRNRLFALSILALAIAMVTLAVACSSSAPAPTAAPKAAEPTKPA
                     AAAPTTAPAAAAPTAAPKAAEPAKAAAPAWTPEKNV"
     gene            complement(620..1270)
                     /locus_tag="VHS06_04570"
     CDS             complement(620..1270)
                     /locus_tag="VHS06_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987427.1"
                     /translation="MTHTLHRQGPVETLEKDYCVLALVARQFDLANDERKASAVKRLQ
                     GICETMAKYGPANLGSLYVKNGTYSHDMSAEEIRAGLKPNGFVLCVYSDQNKFRDCLA
                     ELKEKDYGISITVSGLIGRVFGTCEEVGLMPHTISYSLGVWGKKSLLPKMELLEISSM
                     CGHAMTSPNLVNNAIQDIKAGRTTAQAAAKAIARPCACGCFNTDRAADLLQKAASA"
     gene            complement(1382..2098)
                     /locus_tag="VHS06_04575"
     CDS             complement(1382..2098)
                     /locus_tag="VHS06_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2987428.1"
                     /translation="MRVDEMTCTGCGECLPYCPVGAIELSDGVAVADQEVCVECGVCT
                     RAYNCPSDAMIQLSAEELGFPRGLRRYFSDPSAVSPVTMSAGRGTEECKTNDRTNRVK
                     VGQVGFGIELGRPGISTSFVDVEKVTMKAASIGVEFEQLNPIWPLIKDPKTGTLKDEI
                     KGERVLSLVVEFITDLDKTHKVLRALQEVEQEVDTVFTVAVMCRVSDDRSVPVLPMLQ
                     EMGLQPRPNAKINVGLGRAW"
BASE COUNT          541 a          885 c          832 g          550 t
ORIGIN      
        1 acgttctttt cgggggtcca ggccggcgcc gcggccttgg ccggctcggc agccttgggc
       61 gctgcagtcg gtgcagcagc agcgggggca gtagtggggg cggcagcagc cggcttggtc
      121 ggctcggcag ctttgggagc cgcggtcggc gccggagcag aggacgagca cgctacagct
      181 agggttacca ttgcgatcgc cagagccagg atagacagtg cgaagagacg gttccgcatc
      241 aagcttcact ccttccgtca cagctattcg acatgcctgg atcgatggtt atgcctcgcc
      301 gggattgctg gccggactgg ccccttctcc ggctgcgtca ctggaccagc agccagatca
      361 cctggcgact ccagccgact tctccttccg gctatatccg tgagccgcaa ggttacttcg
      421 gcagatcacc ccctttcaca aagaccccgt cgatatatct agtacccacc acggtggccc
      481 ggacagctgt catcctgagc cgcaggcgaa ggatctctcc cttcacgagg agatgcttcg
      541 ctcagcatga tggtgcgtgt cacctctact gtggcagagc gccaatagga tcggcgcacc
      601 aattgctcgc cacgtcactc taagctgaag ccgccttctg cagtaggtcg gcggctcgat
      661 cggtgttgaa acaaccacag gcacatggcc tggcaatcgc cttggccgcc gcctgcgccg
      721 tcgtgcgtcc cgccttgatg tcctggatcg cattgttgac caggttgggc gacgtcatgg
      781 catggccgca catgctgctg atctccaaca gttccatctt cggaagcaag ctcttcttgc
      841 cccatacgcc gagtgagtag gagatggtgt gaggcatcag cccaacttcc tcgcaggtac
      901 cgaacactct gccgatgagg ccgctcaccg tgatggagat cccgtagtcc ttctccttca
      961 gctcggcaag gcaatcccgg aacttgttct ggtcggagta gacgcacaga acgaaaccgt
     1021 tcggcttgag gccggccctg atctcctcgg ccgacatatc gtgagagtag gtcccattct
     1081 tcacgtagag gctgcccagg ttggccgggc catacttggc catcgtctcg cagatcccct
     1141 gcagccgctt gaccgcggag gccttccgct cgtcgttggc caggtcgaac tggcgagcca
     1201 cgagcgccag cacgcagtag tccttctcca gtgtctcgac cggcccctgt ctatgtaacg
     1261 tgtgagtcat ctctaactcc taacgatcta acgggtgctg agtgctgagt tctgagtgct
     1321 gggcgcgagg tgccggtaat ggcgactctg gcacccgacc ccccgacccc tggcacccgt
     1381 attaccaggc ccgaccgagg ccgacgttga tcttggcatt gggacgcggc tgaagcccca
     1441 tctcctggag catagggagg acagggaccg acctatcgtc gctgacccgg cacatcacgg
     1501 ctacggtgaa cacggtgtcc acctcctgct ccacctcctg gagcgccctg agcaccttgt
     1561 gggtcttgtc cagatctgtg atgaactcga caaccaggga gagcacccgc tcccccttga
     1621 tctcgtcctt gagggttccg gtcttggggt ccttgatgag gggccaaatg gggttcaact
     1681 gctcgaactc gacaccgatg gaggccgcct tcatggtgac cttctcgacg tcgacaaagc
     1741 tggtggagat gccgggcctg cccaactcga tcccgaagcc tacctgcccg accttgaccc
     1801 gatttgtgcg gtcgttggtc ttgcactcct cggtgcctcg accagccgac atggtgacag
     1861 gagaaacggc ggaaggatca gagaagtagc gtctgagtcc cctggggaaa cccaattctt
     1921 cggcggagag ttggatcatg gcgtcgctgg ggcagttgta cgccctagtg cacaccccgc
     1981 actcaacgca cacttcctgg tcggccacgg ccaccccgtc tgaaagctca atcgctccca
     2041 ccgggcagta agggagacac tccccgcacc cggtgcacgt catttcgtca acccgcatcg
     2101 atagcctccg agagtattag ctccgctagg gctaggattg aaagggcgtg aacgagtctg
     2161 aacgaggata gggtcccaga cctgacgcga gccggctcgc ctcgccggca gcgagttcga
     2221 acgaaggccg atcccacgat ggggtcaatc ggggttggtg gacggctaac gagacgagca
     2281 ggaggggctc atcccgctga acgggatgat ctcccgcacg acggcttcta ttatactctt
     2341 gaagaaagcc aagtcaacaa ttggctgctt tcagtgcata gagtcgccag cccaacccga
     2401 cgaaatggcc cgacagggaa accgcagcgt accaccccct tcccgcagcg ccctcgagag
     2461 gcaagtcacc cccgagagtt gtcccttcta cagggatgcc ctcccgcaga atggggctat
     2521 tatactactg ccgtggtctg catcaacctc taagtacttc tatacgacga atcgcccgat
     2581 atcagagagc atttccggcc ggcagtggag acaagatggc cgctgctgat ctcaaacgtg
     2641 gcccgctgcg agaagggcga gggggacagg cgccgcgttg cgatgaatgg atgtcaaatc
     2701 ccttctcgtt gccacattcc tgctccgagt acgtccctac gcatactggt gagtctcgga
     2761 gtagaaggga gattccggtt cagggagagc ggcccgacgc cgccaggg
//
LOCUS       DATALS010000204         2694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_202568, whole genome shotgun sequence.
ACCESSION   DATALS010000204 DATALS010000000
VERSION     DATALS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202568"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1159
                     /locus_tag="VHS06_04580"
     CDS             152..1159
                     /locus_tag="VHS06_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2987429.1"
                     /translation="MKARIVYNPLSGGARGEQELEEVERYLQSLGWEVDRRCSSHDLS
                     PTDLARDGVRDGCEAIIAAGGDGTINQVIQALAGTDVALGAIPRGTGNAWARQVALPW
                     NNPKAAARVLGECETRRIDLGRADGRYFLLMAGVGFDAEVTRQLDRRLKKRYGILAYW
                     IVGVRLAFKWRGVPVTLEFDGQRRRGRALMMVVSNCRLYGGTARIAYRALIDDGLLDV
                     TLLRGSSFLDSVAHLFRVMLGGRVPNPEMEYYRVQRIGIGSSHRLAVQIDGDPAGETP
                     MTFTIAPGALKVLIPRDAPRDIFVDSAKEPTPISPTVVLQQLREVTRNWFREWRRDRE
                     G"
     gene            1135..2313
                     /locus_tag="VHS06_04585"
     CDS             1135..2313
                     /locus_tag="VHS06_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX2987430.1"
                     /translation="MEKRPRGIVLSFVTAPRSGRERGHAYARVRRRLYIGESVLGLAY
                     LLFWLLSGVSQGLQGRLTEAVGSRYATIALYVLVFVVAMAVISLPLSYYSGLVLPRRH
                     GLSHQSVGSWLVDQAKGLGIGVLFAIVALEILYLLIRALPNLWWLPAAGAYLVFAVVL
                     TNLAPVLLLPIFNKLRPLENEDLVQRARRLAEREGIRLVGIFTMDMSRRTSTTNAALT
                     GLGNTRRMIVGDTMVDRYPDEEIETVLAHELGHHVHGDIWKGIALQGLVTLGVLYVIF
                     LILRAFSAPLGLGAPSDIATLPLLALSFTLLNLILSPFLSGFSRWLERKADLYALCVS
                     QPRAFASAMGRLADQNLAENKPPRWADLILADHPSIADRIYLAHNLATDEEHRRQTCA
                     "
     gene            complement(2310..>2694)
                     /locus_tag="VHS06_04590"
     CDS             complement(2310..>2694)
                     /locus_tag="VHS06_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987431.1"
                     /translation="GAAAITTEKVQNLVNDWVRRGDLSAEEGKTIVREYSSRGGRGAT
                     DFVKDMGARGQQQASDLGREARSRMRDLASDLGLATRDEVQRLAARVQALEDQLSKPV
                     ASSGAESGEPEHAEYNIPESPAEGD"
BASE COUNT          420 a          868 c          886 g          520 t
ORIGIN      
        1 tgcgtgatct acttgccggc aagtgactgt gcgtactggg gtcccggttg acacccgcgc
       61 gactcgacga gcatcatagc cgtgtacggt ggcagtgctg agtggagccg gtgccgaaca
      121 tagacgaagg aatcgtgcac gcgtatcagg tatgaaggct cgtatcgtct acaacccgct
      181 ctctggcggc gcgcgtggtg agcaggaact cgaggaagtc gaaaggtacc tccaatcgct
      241 gggttgggag gttgataggc gctgctcctc gcacgatctc tcgccgacgg atctcgcccg
      301 cgatggggtg cgcgatggct gcgaagccat catcgccgcg ggcggcgacg ggacgatcaa
      361 tcaggtgatc caggcattgg ctggcacgga tgtcgctctc ggtgcgattc cgcgcggcac
      421 cggcaacgca tgggcccgcc aggtcgcctt gccatggaac aacccaaagg ccgctgccag
      481 ggtcctgggt gaatgcgaga cccgtcggat cgatttgggc cgggctgatg ggcgctactt
      541 cctgctgatg gccggcgtgg gcttcgacgc cgaggtgacg cgccaactgg accgacgcct
      601 gaagaagcgc tacgggatcc tggcatactg gattgtcggt gtgcgcctgg ccttcaagtg
      661 gcgtggagtg ccggtcacgc tggagttcga tggccagcgg cggcgagggc gtgccctgat
      721 gatggtcgtc agcaactgcc gcctctatgg cggcaccgcc cgcatcgcct atcgtgccct
      781 gatcgacgac ggattgctgg acgtgacact cctgcgaggt tcctctttcc tggactcggt
      841 cgcccatctg ttccgcgtga tgctcggcgg gcgagtgccg aacccggaaa tggagtacta
      901 ccgcgtgcag cgcatcggca tcggttccag tcaccggctg gcggtgcaga tcgacggcga
      961 tccggctggg gagaccccca tgacgttcac catcgctccg ggagcgttga aggtgctgat
     1021 cccacgggac gctccacgcg acatcttcgt ggacagcgcg aaggagccca cgccgatctc
     1081 tcccacggtg gtgctacaac agcttcgtga agtgacaagg aactggttcc gtgaatggag
     1141 aagagaccgc gagggatagt gttatctttc gttaccgctc cgaggagcgg gcgcgagagg
     1201 gggcatgctt acgcccgggt acgccggcgg ctgtacatcg gcgagagcgt gttgggcctg
     1261 gcatatctgc tgttctggct gctgagtggc gtatcacaag gcctccaggg gcgcctgacc
     1321 gaggcggtgg gtagccgcta cgccacgatc gccctctacg tcctcgtctt cgtggtggcg
     1381 atggcggtca tctctctgcc cctgagctac tacagtgggc tggtgctgcc caggcgccac
     1441 ggtctctctc atcagtcggt cgggtcctgg ctcgtggatc aggccaaggg actgggcatc
     1501 ggcgtgctct tcgccatcgt ggcgctggag atcctctacc tgctgatccg cgcgctgccc
     1561 aacctgtggt ggctgcccgc ggcaggcgct tacctggtct ttgccgtggt gctgacgaac
     1621 ctggcgcccg ttctcctgct gccgatcttc aacaagctgc gaccgctgga gaacgaggac
     1681 ctcgtccaac gggcgcgccg cctggcggaa cgtgaaggca ttcggctggt gggcatcttc
     1741 acgatggaca tgagccgtcg cacctcgacc accaatgccg ccctgaccgg cctcggcaac
     1801 acgcggcgca tgatcgtggg ggacacgatg gtcgatcgtt acccggatga ggagatcgag
     1861 accgtgctgg cgcacgagtt ggggcatcac gtgcacgggg acatctggaa gggcatcgcc
     1921 ctgcaggggc tggtcaccct gggcgtgctc tacgtgatct ttctgatact gcgtgccttc
     1981 tcagccccac tcggcctggg agcgccaagc gacatcgcca cgctgccgct gctcgcgctc
     2041 tcgttcacgc tgctgaacct gatcctctcc cccttcctgt ccggcttctc ccgctggctg
     2101 gagaggaagg ctgatctcta tgccctctgt gtatcgcagc cgcgcgcatt cgcctcggcc
     2161 atgggtcggc tggccgacca gaacctggcg gagaacaagc cgccgcgctg ggcggatctg
     2221 atcctggccg atcatccctc catcgccgac cgcatctacc tggcgcataa cctggccacc
     2281 gatgaggagc accgccggca aacgtgtgcc tagtcgcctt cggcggggct ctcagggatg
     2341 ttgtactcgg cgtgctccgg ctcgccggac tcggcgcctg aggaagcaac gggcttggac
     2401 aactggtcct ccagcgcctg gacacgtgcc gccagtcgct gcacctcgtc acgtgtggcc
     2461 agaccgagat cggacgccag gtcgcgcatg cgcgagcgtg cttccctgcc caggtcgctc
     2521 gcctgttgtt gcccgcgcgc gcccatgtcc ttcacgaaat ccgttgctcc gcggcctcca
     2581 cggctggagt actcgcgtac aatcgtctta ccctcctccg ccgagaggtc gccgcgcctc
     2641 acccagtcgt tgaccaggtt ctgcaccttc tctgtggtga tggccgcggc gccc
//
LOCUS       DATALS010000205         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_100026, whole genome shotgun sequence.
ACCESSION   DATALS010000205 DATALS010000000
VERSION     DATALS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100026"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /gene="fdrA"
                     /locus_tag="VHS06_04595"
     CDS             <1..1405
                     /gene="fdrA"
                     /locus_tag="VHS06_04595"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HEX2987432.1"
                     /translation="EAGLLADEVKSAGANDLVVVIEASSAETAQQGLARAEALLSEAG
                     SQAAAREESVNRTMDRALRALPSANMALISIPGMYAKLEAAKALNAGMNVFLFSDNVT
                     LEEELELKQRAVSKGLLMMGPGCGTANINGIALAFGNVIRRGPIGLVGASGTGLQEVT
                     TLIDRAGSGITQAIGVGGRDLSETIGGLMMRQGISMLAADPDTRIIVLISKPPAETVV
                     NKMLDAARAGGKPVVVCFLGGKRSGREGQFTFTTTLEEATEAALRLVGVEEGVSFKMD
                     DRELDQLAAAERMQLSPGQLYLRGLYSGGTLCDEAMWILSSRLPAVYSNIPLRPEWAL
                     EDAWHSRENCFVDMGDEEFTRGRPHPMLDPRLRQERILKEAENPTVAVILLDVVIGYG
                     SHADPAGALAGTIQVAKSRASAQGRYLSVVAHVCGTQQDYQSLQRQEQLLRDAGVLVL
                     PTNSQAARVVAAVVAGS"
BASE COUNT          272 a          473 c          588 g          275 t
ORIGIN      
        1 ggaggccggg ttgctggcgg atgaagtcaa gtctgcgggg gccaatgacc tggtggtggt
       61 gatagaggct tcgtccgcgg agactgccca gcaggggctg gcacgcgctg aggcgttgct
      121 gagcgaggcc ggcagccagg ctgcggcccg cgaggagagc gtgaaccgca ccatggatcg
      181 ggccctcagg gcgttaccgt ctgccaacat ggcgctcata tccatccccg gcatgtatgc
      241 caagctggaa gccgccaagg cgctgaacgc cggcatgaac gtgttcctct tcagcgacaa
      301 cgtgacgctg gaggaggagc tcgagttgaa gcagcgagcg gtgtccaagg gactgttgat
      361 gatgggcccc ggttgcggca cggccaacat caacggcata gccctagcct tcggcaacgt
      421 gatccgccga ggcccgatag ggctggtcgg tgcctccggt actgggctcc aggaggtgac
      481 caccctgatc gaccgcgccg gaagcggcat cacccaggcc atcggtgtgg gtgggcgcga
      541 tctctcggag accatcggcg ggctcatgat gcgccagggc atctccatgc tggcggccga
      601 tccggacacc aggatcatcg tgttgatctc caagcccccc gcggagactg tggtcaacaa
      661 gatgctggat gccgccaggg ccggagggaa gccggtggtg gtgtgtttcc tgggcggcaa
      721 acgatcgggc cgggaggggc agttcacctt cacgaccaca ctggaagagg ccacggaggc
      781 cgcgctgcga ctggttgggg tcgaggaagg cgtgagcttc aagatggacg atcgggaatt
      841 ggatcaactg gcggctgccg agcggatgca gctctcgccg ggtcagctct accttcgggg
      901 attgtactcg ggcgggacgc tgtgcgacga ggcgatgtgg atcctctcca gccggttgcc
      961 ggccgtttac tccaacatcc cgttgcggcc cgagtgggcg ctggaagacg catggcacag
     1021 ccgggagaac tgcttcgtcg atatggggga cgaggagttt acccgtggcc ggccacaccc
     1081 gatgctggat cctcggctgc ggcaggagcg gatcctgaag gaggccgaga acccgaccgt
     1141 ggcggtcata cttctggacg tggtgatcgg ctacggatca catgccgatc cggcgggtgc
     1201 gctggctggg acgatccagg tagccaagag ccgggcgagc gcacaggggc gctatctttc
     1261 ggttgtggcc cacgtctgtg gaacgcagca ggactaccag agccttcagc ggcaggaaca
     1321 gctgctccgg gatgcaggcg tgctggtgct tcccaccaac tcccaggccg cccgcgtagt
     1381 ggcggcggtt gttgcgggca gttaggcgtg gggattgttc ctgcctcaat ccacggaacg
     1441 gtggacctgg tctttaggag cccgggtcgt ccctgacccg ggcggtctct tcgggggccg
     1501 tcggggtctg ggacgacccc gactcctaga acctgtagga cgcgaacgcg gtcggggcgt
     1561 gttctagtcg ggcgggtcgc ccctgacccg cccgtaatcc gggtggcc
//
LOCUS       DATALS010000206         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_155314, whole genome shotgun sequence.
ACCESSION   DATALS010000206 DATALS010000000
VERSION     DATALS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155314"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VHS06_04600"
     CDS             complement(<1..237)
                     /locus_tag="VHS06_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987433.1"
                     /translation="MDNFPNWDSTAMARSQELACLFQQPNSPVQRRYEICRAYFYEGS
                     TADQLAERFQLHVGTIRVIVRDFARDPNINSFFTA"
     gene            909..>1706
                     /locus_tag="VHS06_04605"
     CDS             909..>1706
                     /locus_tag="VHS06_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX2987434.1"
                     /translation="MEPLPEHTLKTIREAARLLTGPKRRRVQAQVALDYLDGNARRAE
                     RVFGWGRQTVQLGLQELRTGITCLDRYTARGRHATEEDHPQLARDIRDLVEPHIQGDP
                     TSPSSFAYTRITAKAVRRALIERKGYRDEDLPCERTLRTLLNRLGYRLRHVQKTRPLK
                     KIKQTDAIFAQVEAAHEQAAASEDTLRISIDTKAKVKVGEFSRDGEARGEEAVKALDH
                     DMEPVATMIPFGILEVVGGLLTILFGTTRLTSDFVVDCLEQWWQERRR"
BASE COUNT          355 a          511 c          556 g          284 t
ORIGIN      
        1 ggcggtgaag aacgaattga tatttgggtc gcgagcgaag tctcggacaa tgacacggat
       61 ggtgccgaca tggagctgga accgctcggc gagttggtcg gcggtcgagc cttcgtagaa
      121 gtaagcgcgg cagatctcgt agcggcgttg cactggggaa ttgggttgct ggaacaagca
      181 cgcgagttct tgggagcgag ccatcgctgt tgagtcccaa ttaggaaagt tgtccacccc
      241 ccgagagaca tcctgggagg ggtttgatcc cagggttagg ggaagggtgg cgtgcaagaa
      301 cagcaagaaa tgaagggggt ttcggcagga tcgacagggg aatggaggag aagcaaagga
      361 ggggcggaga ggacgggaat cgcggacaac ttgcctatgg ggcactcaag tcgcggacct
      421 caggagccgc aaaaaccggg ggaaaacggc acttttgacc gtgcacagtt gggtgagaaa
      481 taggaaagtt gggcgttcgc gaaaatcggc gaagaaagga caggccggat atcaaagccg
      541 tgcccatctg gccgccggtc tggcgcacca attggccgag ggcttgccgg agcggccttt
      601 ctggttggtc gccgatgcgg cctacatcaa ctccgtcttg acgaaggacc gccccaagaa
      661 ccttcaggtg ctcgggccgc tccgctggga tgcggcgttg tacgccgtgc ccggcccctg
      721 cagcgggacg ggacgcccgc ccaagaaggg ccagcggttg gccaccccgc tgaagatgat
      781 cgaggacacg acaaccttcc cggcccggtt aggcgatagg aaagagatag cctacccctc
      841 ctctccggcc agccatatac tctctccttt gtcattcttc tcgtgtcctt tcccagcctc
      901 ggtgaaccgt ggaaccgctc ccggaacaca ccctcaagac tattcgcgag gccgccagac
      961 tcctgaccgg tcccaaacgg cgacgcgtcc aggcccaagt cgctctcgat tacctcgacg
     1021 gcaacgcgcg tcgcgccgaa cgcgtcttcg gctggggacg tcagaccgtc caactcggcc
     1081 tccaggaact gcgcaccggg atcacctgcc tcgatcggta caccgctcgc ggacgacacg
     1141 ccacggaaga agaccacccg cagttggctc gagacatccg cgacctcgtc gaaccccaca
     1201 tccagggcga tcccacgtcc ccctcctcgt tcgcctacac gcgcatcacg gccaaggccg
     1261 tccgccgtgc cctgatcgag cgcaagggtt atcgggacga agacctgccg tgtgagcgca
     1321 ccctccgcac tctcctcaac cgtctgggct accgcctgcg tcacgtgcag aagacccggc
     1381 ccctcaagaa gatcaagcag accgatgcca tctttgccca ggtcgaagcg gcgcatgagc
     1441 aggcggcggc ttcggaggac acgctccgga tctcgatcga caccaaggcc aaagtgaaag
     1501 tcggggagtt ctctcgtgac ggtgaggcac ggggcgagga agccgtgaag gcgctggacc
     1561 acgacatgga gcccgtggcg acgatgatcc ccttcgggat cctggaggtc gtgggtggtt
     1621 tgctgaccat cttgttcggg acgacacgct tgacgagtga cttcgtggtg gactgcttgg
     1681 agcagtggtg gcaggagagg cgcagg
//
LOCUS       DATALS010000207         1543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_54689,
            whole genome shotgun sequence.
ACCESSION   DATALS010000207 DATALS010000000
VERSION     DATALS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54689"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /gene="murJ"
                     /locus_tag="VHS06_04610"
     CDS             complement(<1..1348)
                     /gene="murJ"
                     /locus_tag="VHS06_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX2987435.1"
                     /translation="MSSAEVAPKGKSLALGAAILMAGFVASRMLGLVRTMVISSQFGA
                     SREYEAYLAAIRVSDTLFQVLAGGAVASAFIPVFAGYLARKEPERAWRMASSLITVAA
                     VVMTPISLLLMILAPQVMSIITPGWHGDEANQQLAANLARILLFSPVIFAVSTFVASI
                     LNSFNRFLLASLAPMMYNLSIIGGALLLGPRFGVYGLAVGAAVGALLHLLVQIPGLVR
                     VKMDFHPMLDLAHEGVREVGRLMVPRTLGLGVVQVNYLVNVVLASRLEAGSLAYLDYA
                     WMLTMLPLGVFAMAISTAVFPTLAEQSALDRLDDLRSTFTTALRLILYLTIPASVGLI
                     MLGEPIVRLLLQRGDFSPEATRATAYALGFFAIGLAGQATVEIVDRVFYALHDTRTPV
                     SVAFGAFLINVILSLVLMNYLSFGGLALANALAGLVEGGTLVMLLGRRLEGVRLSPL"
     gene            complement(1351..>1543)
                     /locus_tag="VHS06_04615"
     CDS             complement(1351..>1543)
                     /locus_tag="VHS06_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987436.1"
                     /translation="CYLHGSLLPKNPCLTDHLLSVALRRRYGGEVSLQPLDDTLEERA
                     HEAVIARIRKLGKLSTGVV"
BASE COUNT          335 a          482 c          508 g          218 t
ORIGIN      
        1 tcagagggga aaggcgcaca ccttccaggc gacgccccag cagcatcacg agggtcccgc
       61 cctccaccag cccggccaac gcattggcca gcgccagccc gccgaaggag aggtagttca
      121 tcaacacgag ggagaggatc acgttgatca agaaggcgcc gaaggccacg gatacggggg
      181 ttcgggtatc gtggagagca tagaagaccc ggtcgacgat ctccacggtg gcctgcccgg
      241 ccaagccgat ggcgaagaag cccagggcgt atgccgtggc gcgagtggcc tcaggagaga
      301 agtcgccgcg ctgcaacaag agacgaacga tcggctcgcc cagcatgatc agaccgacgc
      361 tggccggtat ggtcaggtag aggatcaagc gtagtgcggt agtgaaggtg gacctcagat
      421 cgtccagccg gtcgagggcg ctttgctcgg ccagggttgg gaagaccgcc gtggatatcg
      481 ccatggcgaa caccccgagg ggaagcatgg tgagcatcca ggcgtagtcc aggtaggcca
      541 ggcttcccgc ctccagcctg gaggccaaca cgacgttgac caggtagttg acctgaacga
      601 ccccaagccc cagggttcgg ggcaccatca gccggccaac ctctcgcacc ccctcgtggg
      661 caaggtccag catcgggtga aagtccatct tcactcgaac gagtccgggg atctgcacca
      721 acagatggag cagcgccccc acggctgcgc cgacggcaag tccgtagaca ccgaagcgcg
      781 gccccagcaa cagggccccg ccaataatgg acaggttgta catcatggga gccagggagg
      841 ccagcaagaa gcgattgaag gagttcagga tgctggccac gaaggtggag acggcaaaga
      901 tcaccggcga gaacagcagt atccgggcaa ggttcgccgc caactgctgg ttagcttcgt
      961 cgccgtgcca ccccggtgtg atgatggaca tcacctgggg cgcaaggatc atcagcaaca
     1021 gggagatggg ggtcatcacc acggcggcaa cagtgatgag ggatgaggcc atgcgccagg
     1081 cacgttccgg ctccttgcgg gccaggtagc cggcgaagac cggaatgaag gccgaagcca
     1141 cggcaccacc ggccagcacc tggaaaaggg tgtcggagac tcggatggcg gccaggtatg
     1201 cctcgtactc gcggctggcg ccgaactggc tgctgatcac catggttcgc accagtccca
     1261 gcatgcggct cgccacgaaa cccgccatca ggatggcggc acccagcgcc aggctcttcc
     1321 ccttcggcgc gacctcagcg ctggacaaag ctaaaccacc cccgtgctca gcttccccag
     1381 cttcctgatt cgggcaatca ccgcctcgtg ggcacgctcc tccagggtgt cgtccagtgg
     1441 ctgcaggctc acctctcctc cgtaccggcg acgcagtgcg acggaaagca gatgatccgt
     1501 gaggcagggg ttcttgggca gcagcgagcc gtggaggtag cat
//
LOCUS       DATALS010000208         3526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_155541, whole genome shotgun sequence.
ACCESSION   DATALS010000208 DATALS010000000
VERSION     DATALS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155541"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VHS06_04620"
     CDS             <1..983
                     /locus_tag="VHS06_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987437.1"
                     /translation="QIPGVYPLSDLTARTFASGLDTHGESDPSFLSKLNDEILSVKDL
                     TTILEMHQEERQAVLAQLREIYDGRFDKVWGTGKELHWQGRLGFLAGVTPVIDQHYAV
                     MGLLGPRFVQLRLRGGDRQQVAIKAIANSRQEEMRQELAGATAAFVSAIPNRPPVVHE
                     CMRPWLAQVADLATKSRSPVVRDGWRREIEYAPEPESPARLARQLYALLQGVTLVCGR
                     EQAGADDARRVARVALDCIPAIRRSVLETLVKTESFLKTSDVSNSAQHANTTVRRALE
                     DLHALGLVMCRKGGEGRPDEWRLTDECRAMMAAATTVPEISEGCPNRLSD"
     gene            1606..2112
                     /locus_tag="VHS06_04625"
     CDS             1606..2112
                     /locus_tag="VHS06_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987438.1"
                     /translation="MAEESVDIDAVKAALTAAQNGDQSALVSLRQSFEVESRLWKTLD
                     KLTARSENTMIELLVGNNKLLAEKMRLGLAALKRELSGPNPTLLEELLVERIVVCWLD
                     VHYADFVSTTCLKVNGTDKQVAFCQKYQNHAQRRYLAAVKSLALVRRLLTPQVQVNIG
                     ARQVNVAQ"
     gene            2204..2335
                     /locus_tag="VHS06_04630"
     CDS             2204..2335
                     /locus_tag="VHS06_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987439.1"
                     /translation="MASKFIQADTAVLINGEGQDSKVAQEIASAMVKDSTSIRMLFG"
     gene            2745..3449
                     /locus_tag="VHS06_04635"
     CDS             2745..3449
                     /locus_tag="VHS06_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2987440.1"
                     /translation="MRHPAHVWVDPTFAAESLERLREEYVGFLKGRARSTSSATIDKY
                     SKTLLSFIRFLDRVGEPLVLSNLTSDTVSRWVTEQRERGMSEEGIASRLSALKVFSKR
                     FVFEHLELTTVDLLRKVARITPPERSYPVLTETEQARLLDAFDRGTYEDLRNRAMMAV
                     FLATGMRFSEVLNIDMASFDRVTAEIRVIGKGNKERWVRLSPGALREVKPYLKRRPDH
                     GPSDRMWLTEDGRALT"
BASE COUNT          754 a          887 c         1150 g          735 t
ORIGIN      
        1 ggcagatccc gggtgtctat cccctgtcgg acctgacggc tcgcaccttc gcctctggtc
       61 tggatacgca cggcgaaagc gatccttctt tcctctcgaa gctcaatgac gagattctct
      121 ctgtgaagga tctgacgaca attctggaga tgcaccagga ggaacggcag gccgtgttgg
      181 cgcagctcag ggagatctat gacggtcgtt ttgacaaggt gtggggtact ggcaaggagt
      241 tgcactggca gggccggctc ggcttcctag ctggtgtcac tcctgttatc gaccagcact
      301 atgctgtgat ggggctgtta ggacctcgct tcgtgcagct tagactgaga gggggcgacc
      361 ggcagcaggt ggccatcaag gcaatagcca actcccggca ggaggagatg cgccaggaac
      421 tggcaggagc gacggctgcc tttgtctcgg ctatccctaa cagacctccc gttgtgcatg
      481 aatgcatgcg gccgtggctc gctcaagtgg cggacttggc gactaagagt cgctcgccag
      541 tggtacggga cggctggaga cgcgaaatcg agtatgcacc ggagccggag agcccggcgc
      601 ggcttgccag gcagctatac gcattgcttc agggggtcac cctggtgtgt ggtcgggagc
      661 aggccggagc tgatgatgcc aggcgagtgg cgcgagttgc cctcgattgt attccggcga
      721 tacgccggtc ggtgttggag accctggtga aaaccgagtc tttcctcaag actagcgatg
      781 tctctaacag cgctcagcat gccaacacca ccgtgcgcag agctttggag gacttgcatg
      841 ccctcggtct agtgatgtgc cgaaaagggg gagagggccg gcccgacgaa tggaggctga
      901 ccgacgagtg ccgagccatg atggcggcag caacaaccgt tcccgagata tcggaggggt
      961 gtcccaacag actgtccgat tagcatactc acgagggtga ttgatgactg cgttgtgtac
     1021 cacctcggcg atcgtctggc cctcgaagtc gaacgggtag gtgcgtggcc aggagggaag
     1081 gaccccactc ctctttctgt cgataggctc tagcgatgcg gggcatctca ggaagcggcg
     1141 tggcgcgcct tgaacgaggt tcgagacgga gtgaaaacgg acgcctaggt ggggctggac
     1201 ggccaacaag tgatcatgct tggaggcgtc gaccgcgttg gcctgcggcg gaacgtcgcg
     1261 gttaggctcc taccggggca ctgatgggga ggtagcacaa ttccatggct tgggagaagc
     1321 gctcatcagg ccggctctac tactaccgga gtcgaagagt aggtcaccgg atagtgaagc
     1381 actacttggg ctgcgggcct gtcgcgaagt tggccgcggc catggacgcc gaagcccgca
     1441 agacacggaa agcaggcatg aaacaggcta gggaggaaca atcacgagtt aggcacgtaa
     1501 gttcacaggt ggagcggtta tctgaggctg ccgatctcat gacaaagatt gctctagtcc
     1561 tcgcggggta ccaccaacac caccgaggtg agtggaggca ttgccgtggc tgaagaatcg
     1621 gtcgacattg atgcggtgaa ggctgcgctg actgctgccc agaatggcga ccaatcggcc
     1681 ttggtctcgc tgcggcagtc attcgaggtt gagtctcgac tttggaagac gttggacaaa
     1741 ctgacagcca gatccgagaa cacgatgatt gagttgctgg ttggtaacaa caagttgctg
     1801 gcagagaaga tgcggctcgg gttggcagcc ctgaagcggg aactgtctgg accgaatccg
     1861 acactgctgg aggagttgtt ggtagagagg atcgtcgtct gctggctgga cgttcattat
     1921 gccgactttg tctccacgac ctgtctgaag gtgaatggca ccgacaagca ggtggcgttc
     1981 tgccagaagt accagaatca cgcccaacgc aggtatctgg ccgccgtcaa atcgttggca
     2041 ttagtgaggc gactgctgac cccgcaagtg caagtcaata ttggtgcccg gcaagtcaat
     2101 gttgcgcaat agcagcgcgg tccactgatc aaccggccag ttctcgcgga atatgtgatc
     2161 tttagccgct tgataattct tcagagtttt gtaggaggag aatatggctt cgaagttcat
     2221 ccaagctgac acggcggtcc tgatcaatgg agagggacag gactccaagg tggcccagga
     2281 gatcgcctcg gcgatggtga aggatagcac cagcattcgg atgctgttcg gttagcgcgg
     2341 atggctcggg gggcggtttg gcggtttgca ccattactgg gtgtctccct agtagttgga
     2401 aatgtgttgg ttgggtctag catcaattcc atcgtaggcc gttcgattgt gattgccgcc
     2461 gcgacccaag catctgagtc gactcctagc gccggaatgc cggagactca ggttagcgat
     2521 cgctacaggg ccgcgctggg cgcaagcata aggttcagca attctactca ggagatccag
     2581 atcggcgtac aggttgcgga aggaggcgtt cgacggtgcg gttggctgga acggccatat
     2641 cacatgatag tctgctcaag ttgccacgcc ccgcggcagt taacataaag tctgctacaa
     2701 tagactcgga ccgggcggtc ggcaaggtag aaagacatgg cgacatgaga catccagccc
     2761 atgtttgggt tgatcctacc tttgcagccg agtcactgga gcgacttaga gaggagtatg
     2821 ttggttttct gaaaggtcgg gctagatcca cctcttcagc gaccatcgat aagtacagca
     2881 agacgctgct gagcttcatt cgcttcctgg atcgtgtcgg agagccgttg gtcttgagca
     2941 accttacttc cgatacggtc agccgttggg tgacggaaca gagagaaagg gggatgagtg
     3001 aagagggcat cgcttccaga ttgtcagctc tgaaagtctt ctccaagcgc ttcgtgttcg
     3061 agcacttgga gttgactacg gtagatctcc tgaggaaggt tgcgaggatt actccgcccg
     3121 agcgctccta tccagtactg actgagactg agcaggcgcg cctgttggat gcattcgata
     3181 gaggcactta cgaggatctg cggaaccggg caatgatggc tgtctttctg gcgacgggga
     3241 tgcgtttctc tgaagtgttg aacattgata tggcgagctt tgacagggtg accgccgaga
     3301 tcagagtaat cgggaaggga aacaaggagc ggtgggtgcg gctcagccct ggtgctcttc
     3361 gagaagtgaa gccctatctg aaaaggcgcc ccgatcatgg cccttcagat cggatgtggc
     3421 tgaccgagga tggcagagcg ctgacctgag tctccggcat tccggcgcta ggagtcgact
     3481 cagatgcttg ggtcgcggcg gcaatcacaa tcgaacggcc tacgat
//
LOCUS       DATALS010000209         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_54771,
            whole genome shotgun sequence.
ACCESSION   DATALS010000209 DATALS010000000
VERSION     DATALS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54771"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1837)
                     /locus_tag="VHS06_04640"
     CDS             complement(<1..1837)
                     /locus_tag="VHS06_04640"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX2987441.1"
                     /translation="MLGSGLITGLAYFYTDWLWFKDLGYVSVLWTTLATTWAMRLLVG
                     AVFFLFLFFNLLLTKPVVGRALDRLEGTPLPAILTASRVTQAFAAVSAIVAVFAAMAS
                     SLYWEVVQKFLHGGTWGISDPLFGRDLGFYVFRLPFFKYLYSTGMGLLIVTLLAVGAV
                     YVLAGQLTFKGFTPSFQGRARHHLAILVAALFALKAWGYWLSQAHLLYSTRGVVFGAG
                     YTDVHVNLLGYRILLVVALACAAIVMLSWVAKSGRWLVAGVFLLAAVSLLLGSAYPAM
                     IQQFVVEPNELVKETPYIRQNIKYTRIGWGLDRVEERPFAVRNDLTYQQLQTEQETLQ
                     NIRLWDWRPLQATYGQLQGIRPYYDFLGVDVDRYRIGGQLRAVTLAARELNQEKIPAQ
                     ARTWLNLHLKYTHGFGLVMSPVNQVTQEGMPELWVKNLPPESPVGVKITRPEVYFGEE
                     TGDYVITNTRSPEFDYPLGDKNAYTQYQAKTGVSLASPLTKLAFAARFGTLNLLLATD
                     ITSNSRLQLYRNIKERVARLAPFLRYDSDPYLVVMDGKLYWMQDAYVTSDLFPYSEPM
                     GDWGNYVRNSVKVVIDAYSGATTFYLWDEREPLAATYARIFPHLFK"
BASE COUNT          384 a          574 c          584 g          317 t
ORIGIN      
        1 gcttgaacag atgcgggaag atgcgggcgt aggtcgcagc gaggggctcc cgctcatccc
       61 acaggtagaa ggtcgtcgca cccgagtagg cgtcaatgac caccttgacc gagttgcgca
      121 cgtaattgcc ccagtctccc atcggctccg agtacgggaa gagatcgctc gtgacgtagg
      181 cgtcctgcat ccagtaaagc ttgccgtcca tgaccacgag gtaggggtcg ctgtcgtaac
      241 ggaggaaggg agcgagccgt gccacccttt ccttgatgtt acggtacagc tgcagccggc
      301 tgttgctggt gatatcggtc gccaggagca ggttgagcgt cccgaagcgg gcggcgaagg
      361 ccagcttggt gaggggtgaa gccagggaga cccccgtctt ggcctggtac tgcgtgtacg
      421 cgttcttgtc gcccagcggg tagtcgaact cgggcgaacg ggtgttggtt atgacgtagt
      481 ccccggtctc ctcgccgaaa tacacctcag gccgggtgat cttgacgccc accggcgact
      541 cgggcggcag gttcttgacc cacagctccg gcatcccttc ctgggtcacc tggttgaccg
      601 gactcatgac cagtccgaac ccgtgtgtat acttcaggtg gaggttgagc caggttcgtg
      661 cctgcgccgg gatcttctcc tggttgagtt cacgagcggc cagggtcacc gccctcagct
      721 ggcctccgat gcggtaccgg tccacatcca ccccgagaaa atcgtagtag ggccggatcc
      781 cctgcaactg tccgtaggtg gcctgaagcg gccgccagtc ccagagccgg atgttctgga
      841 gtgtttcctg ttcggtctga agctgctgat aagtgaggtc gttccgcacc gcgaagggtc
      901 gttcctcaac ccggtccagc ccccacccaa tccgggtgta cttgatgttc tggcggatat
      961 acggcgtctc tttgaccagc tcgttcggtt cgaccacgaa ctgttggatc atggccggat
     1021 aggcgctccc caacagaagt gagaccgcag ccaagaggaa tacccccgcc accagccacc
     1081 ggccgctctt cgctacccaa gagagcatca cgatcgccgc acaggccagg gccacgacga
     1141 gcaggatccg gtaacccagg agattcacgt ggacgtccgt gtagccagcc ccgaagacca
     1201 cgccccgggt tgagtagagc agatgagcct ggctcagcca gtacccccag gccttcagag
     1261 cgaagagggc agccacgagg atggccagat gatggcgggc gcggccttga aacgaagggg
     1321 tgaaaccctt gaacgttagt tgcccagcca gaacgtacac cgcccccacc gccagcagcg
     1381 tgacgatgag cagacccatc cccgtactgt acaggtattt gaagaagggc agccggaaca
     1441 cgtagaaacc cagatcgcgg ccgaatagcg ggtcgcttat gccccaggtc ccgccgtgaa
     1501 ggaacttctg gaccacttcc cagtagagcg aggaggccat ggcggcgaag accgccacga
     1561 tcgccgagac cgcggcaaaa gcctgcgtca cccgggaggc ggtgaggata gccggcagcg
     1621 gcgtcccttc aagccggtcg agcgccctcc ccaccaccgg tttcgtcaag aggaggttga
     1681 agaagaggaa gaggaagaag accgctccca ccaggaggcg catcgcccag gtggtcgcca
     1741 gcgtggtcca gagaaccgag acatacccga ggtctttgaa ccacagccag tccgtgtaga
     1801 agtaggctaa tcccgtgatc aagccgctgc cgagcaatac caccaggatg agcagcgtc
//
LOCUS       DATALS010000210         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_280497, whole genome shotgun sequence.
ACCESSION   DATALS010000210 DATALS010000000
VERSION     DATALS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280497"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1944
                     /locus_tag="VHS06_04645"
     CDS             <1..1944
                     /locus_tag="VHS06_04645"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2987442.1"
                     /translation="AESAPAKEQTVDRGDDHPSPSDDVATEDAAMVPEPEEPPTSLAA
                     LPYSAVDAQPTGIESEGVNPVEVEPAGVEPVSEPPEAEDETESQEVPVDGEREAAAAE
                     SSTQEAEPEIQPEETAVELEPLEKPEEGEPAAPPLLETVEAESEPEPESLQEEALTQV
                     EPELEPEPEPELEPEPEPEPEPEPEPEPELEPETEPEPEPLLEETLAQAEPEPEHRPE
                     VDSTQTEPETEPEASEYGAEPEVETVEPEVAPPTVEDLLQQAIEARTEGRLEDALSIL
                     QGAAEPGTMPAGLLLELASVQAQLGRTDDSIASYTRVLELDPGAVEARLQLARLLRGA
                     GSRDEAERHCFVSLNASKTGDAEAAGKAVSLLLEIVREKAAEGDLPTAVESLLLLRSA
                     TACPQQPPLLSERAASASLELLGICGGEHLEELSVLPGEVLAGVVQQLKNAEDLALHG
                     QLRSAADILYELIAENPDFLPAQSLLGRVVAAAGSRDAALNRSDRVAVLYDMRGSPHQ
                     GMELYRWRVVDGLADADARARLLSELKDQNRLDEAEVLQSGCLPGRWKVERATSAFEL
                     LPLPEPLEDLLRRAEAAFAGGDSTEAIQLLHSGLEDAPQVDTPTVAALLRMLQVSGES
                     QVDGEQLWGALDEMGLPQGLADW"
     gene            1986..>2591
                     /locus_tag="VHS06_04650"
     CDS             1986..>2591
                     /locus_tag="VHS06_04650"
                     /inference="COORDINATES: protein motif:HMM:NF039908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEX2987443.1"
                     /translation="MTTFLQSALSTLSRLDFRAVIDIVIVAVIVYWILALIQGTTAVA
                     LVRGMATLILLAAVLGNVLDLVVLNWVLRNAIPAFLVSIPILFQPELRRALERLGRTS
                     RFVARAGPSNETAKALEQIAVACRRFSDRKWGALIVLERDTALGEYIDTGVEIDGLVS
                     VDFLMSIFFPNAQLHDGATIIRGDRVVAAACLLPLTETLLSD"
BASE COUNT          477 a          706 c          917 g          491 t
ORIGIN      
        1 gccgaatcgg cgccagccaa agaacagacg gtagatcggg gcgacgacca cccctccccc
       61 tccgacgatg tggcgactga ggatgcagcc atggtcccgg agccggaaga gccgccaacg
      121 tcactcgctg cattgccgta ctcagcggtt gatgcccagc ccacgggaat cgagtccgag
      181 ggtgtcaatc ccgtcgaggt cgagcccgcg ggtgtcgagc ctgtgtctga gccgccggag
      241 gctgaggacg agacagagtc tcaggaagtg cccgtggatg gtgagcggga ggcggcagct
      301 gctgagtcga gtactcagga ggctgaaccg gagattcagc cggaggaaac ggccgtcgag
      361 ctggaaccgt tggagaagcc tgaagagggt gagccggctg ccccacctct gttggagaca
      421 gtggaagccg agtcggaacc cgaacccgaa tcgctgcagg aggaggctct aactcaggtc
      481 gagccagaac tggagccaga accggaacca gaactggagc cagaaccgga gccagaaccg
      541 gaaccggaac cggagccaga accggagctg gaaccggaga cagaaccgga acccgagcca
      601 ctgctggagg agactctcgc ccaggccgag ccggagcccg agcatcggcc ggaggtggac
      661 tccacccaaa ctgagccgga aaccgagcct gaagcgtctg agtatggtgc agagcctgag
      721 gtcgagaccg tcgagccgga agttgcaccg ccgacagtcg aggatctgct gcagcaggcg
      781 atcgaggcaa ggacggaggg gagactggag gatgcgttgt cgatcctcca gggtgccgcg
      841 gagcctggta ccatgcctgc aggcctattg ctggaattgg cgtcggtcca agcgcagttg
      901 ggccggacgg atgactcgat tgccagctat acccgcgtct tggagttgga tcccggagcc
      961 gtagaagcac gtttgcagtt ggctcgcctg ctcaggggcg cggggagtcg ggatgaggca
     1021 gagcggcatt gcttcgtgtc gctgaatgcc tcgaagaccg gcgacgctga agcagcagga
     1081 aaggcagtct cccttttgct ggagattgtg cgggagaagg ccgctgaggg tgacctaccg
     1141 acagccgtcg agagtctcct gctgcttcgt tctgccactg cttgtccaca gcagcctccc
     1201 ttgctatctg agcgagcggc ctcggcctcc ctggagttgc tggggatttg cggcggcgag
     1261 cacctggagg agctctcggt tcttccgggg gaagtcttgg caggggtggt tcagcagttg
     1321 aagaatgccg aggatctggc cctgcatgga cagctgcgtt cggccgccga tatcctctac
     1381 gagttgatcg ctgagaatcc ggatttcttg cccgcgcaat cgttgctggg gagagtagtg
     1441 gcggcggcgg gatcgaggga tgcagcgctg aatcgctcag atcgggttgc cgtcctgtat
     1501 gatatgagag gtagtccgca tcagggcatg gagttgtatc ggtggcgggt cgtggacggg
     1561 ctcgccgatg ccgacgcccg ggcacgccta ctatcggagt tgaaggatca gaaccggctt
     1621 gatgaggcag aggtgttgca gagcggctgc ctcccgggac gatggaaagt cgagcgggcg
     1681 actagcgcgt tcgaattgtt gcctcttcct gaaccgttgg aggatctgtt gcggcgtgct
     1741 gaggcagcgt tcgccggtgg agactcgaca gaggcgatcc agttgctcca ttctggtctg
     1801 gaggatgcgc cccaggtgga tacgcccacc gtcgcggccc ttctaaggat gctgcaggtg
     1861 agcggggagt cgcaggtcga tggggagcag ttgtggggcg ccctggacga aatgggcctc
     1921 cctcaggggt tggcggattg gtgatagttg cgttgaggta gtcccacgga agggtgttcc
     1981 ggagtatgac cacttttctt cagtcggcgc tgtcgacgct ctcgcgtctc gactttcgcg
     2041 ccgtcatcga catagtaata gttgcggtga tcgtgtactg gatcctggcc ttgatccagg
     2101 gcacgacggc ggttgcgctg gttcgcggca tggccaccct gatcctgctg gcggcggtgc
     2161 tgggcaatgt cctcgacctg gttgtcttga actgggtgct gcggaacgcc atccctgcct
     2221 tcctggtctc cattccgatc ttgtttcagc ctgagttacg cagggcgttg gagaggttag
     2281 ggagaaccag ccgctttgtg gcccgggccg gtcccagcaa cgagacggcg aaggccctgg
     2341 agcagatcgc ggtcgcttgc cgccgctttt ccgacagaaa gtggggggcg ctgatcgtgt
     2401 tggaacgcga tactgcgttg ggggagtaca tcgacaccgg ggtcgagatt gatggtctgg
     2461 tgtccgtgga cttcctgatg tccatattct tccccaatgc gcagttgcac gatggtgcga
     2521 ccataataag gggggatcgg gtggtggcgg ccgcctgcct cttgccgttg accgaaaccc
     2581 tgctctcgga t
//
LOCUS       DATALS010000211         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_203550, whole genome shotgun sequence.
ACCESSION   DATALS010000211 DATALS010000000
VERSION     DATALS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203550"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VHS06_04655"
     CDS             <1..183
                     /locus_tag="VHS06_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987444.1"
                     /translation="EVGPLHRRFLDLFADAGRRTERVGDMVVRLGLRTVLELMELDPD
                     PAVLDEPTHQLFIVWP"
     gene            338..1435
                     /locus_tag="VHS06_04660"
     CDS             338..1435
                     /locus_tag="VHS06_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2987445.1"
                     /translation="MKRSSLPVGIMPDPKAQLPGLLLRNEGKWVRHEGVRPGVYRHVS
                     STGEECLTVRVQMPPNGLLSSGSYRRLALLVESHALVGRRTSRNAFELVGVDPSRVDC
                     LVKELETLGYPVGGTGHGLHQIKSCGGFVACQNAAVDAPTIAKLLGDHLFEDVTAQHY
                     PSWVKLSVTGCPNQCGGGIEADIGIMGAFKGLPQVDDVKLVESKCDVPLLCFWCPTGA
                     IKPKPVRGGMSVEINADRCTRCTSCANVCPAAIEMGGERGVAIAVGGCSGNSSSGPRL
                     ARLLVPFLSIEDPMECDGVIDVVDRVLKVWTDGAESGERLGDFIDRVGWPAFLRRVGI
                     AFDPNLIDNFVATSVRRNLQMRWDSGPGETR"
     gene            1452..1634
                     /locus_tag="VHS06_04665"
     CDS             1452..1634
                     /locus_tag="VHS06_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987446.1"
                     /translation="MEILVQCPRCGYVMSVFDEETLRANRALKRAYEHTLKNPVLCAG
                     CGRWVQRPKVEVKEDS"
     gene            1631..>2696
                     /locus_tag="VHS06_04670"
     CDS             1631..>2696
                     /locus_tag="VHS06_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEX2987447.1"
                     /translation="MSLPRLVVAAPHGRSGKTIVTIGLVAALAARGIAVQPFKKGPDY
                     IDPGWLSDASGRPCRNLDLFMMSEEVALRAFQRGIEGAEVAVVEGAMGLYDGLDLEGS
                     GSTAQLARLLSAPILLVVDATRMTRSVAALVTGFQRFEPGVNVAGVVLNNIARPRHEE
                     MLVRSIQHYCGIPVLGCIPRSESLAIPDRHLGLIPVAEDHGLTSIIQNARRIAEERLD
                     LGRILAVAGSAPELAPPMLGVAQCRKRLVRMAVLLDRVFSFYYPENLEALEAAGAVLE
                     HVDSLSDPSLPDVDGLYVGGGFPELFMEQLEANESLRRDIRARIDGGGTNIGAGGATR
                     ASICVDLDGHTAPDRLRLDGA"
BASE COUNT          454 a          746 c          933 g          563 t
ORIGIN      
        1 gaggtggggc cgctacacag acggtttctt gacctcttcg ccgacgctgg gaggcgtacg
       61 gagcgggtgg gcgacatggt ggtgcggctc ggtttgcgta ctgttctgga gttgatggag
      121 ttggatccgg acccggcggt gctggatgag ccgacccacc aactgttcat tgtgtggccg
      181 taggtgtcgg ctcaatgacg gatcgagtga tggtggggcg gacctgtgtg tccgccctcg
      241 gctctgggcg gacacacagg tccgcccctc ccgtgcgttg acgggcgggt gccgattacc
      301 ggctattgga tagacactgt gtgtgggctc gtgaggaatg aagaggtcta gtctccctgt
      361 tgggatcatg cccgatccga aggcgcagct tcccggattg ttgttgcgga acgaggggaa
      421 gtgggttcgc cacgagggcg tgcggcccgg ggtatatagg catgtctcct cgaccgggga
      481 ggagtgtttg acggtgaggg tacagatgcc ccccaacgga ttgctctcgt ccggcagcta
      541 tcgaaggttg gcattgctgg tcgagagtca tgctctcgtg gggcggcgga cctcccgcaa
      601 cgccttcgag ttggtcgggg tggatccatc gagggtagac tgcctggtga aggagttgga
      661 gacgcttggg tatcccgtcg gcggtacagg gcatggacta caccagatca agtcgtgcgg
      721 tggtttcgtg gcgtgccaga acgcggcggt agatgcccct accatcgcga agcttctcgg
      781 agaccatctg ttcgaggatg tgacggcgca gcactacccc tcctgggtga agctttcggt
      841 gaccggatgc cccaaccagt gcggtggagg catcgaggcg gacatcggca tcatgggggc
      901 tttcaaggga ctaccgcagg tcgacgacgt caagctggta gaatcgaagt gcgatgtgcc
      961 gcttctctgt ttctggtgtc ccaccggcgc catcaagccg aagcctgtcc ggggcggtat
     1021 gtccgtcgag atcaacgcag atagatgcac gcgttgcacc tcctgcgcca atgtttgtcc
     1081 cgccgccatc gagatgggtg gagagcgtgg ggtggctatc gccgtgggag ggtgctccgg
     1141 gaacagcagc agcggacctc gtctagctcg tctgttagtg cctttccttt ctatagagga
     1201 cccgatggag tgcgacggcg taatcgatgt ggtcgatcgg gtgctcaagg tgtggactga
     1261 tggggccgaa agcggcgagc ggttgggcga tttcatcgac cgtgtggggt ggcccgcctt
     1321 tctgcgcagg gtggggattg cctttgaccc caacctgatc gacaatttcg ttgccacctc
     1381 cgttcgtcgc aaccttcaga tgagatggga cagcggaccg ggggaaaccc ggtaagccga
     1441 ggtgggcggc cgtggagata ctggttcagt gcccgcgctg cggctacgtg atgtcggtgt
     1501 tcgacgagga aacccttcgg gccaacaggg cgctgaagag ggcctacgag cacacactca
     1561 agaaccctgt gctgtgcgcc ggatgtggcc ggtgggtgca gcgaccgaaa gttgaagtca
     1621 aggaagactc atgagtttgc ccagactggt tgtcgctgcc ccccacggga gatcgggcaa
     1681 gacaatcgtg accatcggct tggtcgctgc tcttgccgcc cggggcatcg cggtccagcc
     1741 tttcaagaag ggaccggact acatagaccc gggctggctg tccgatgctt ccggacggcc
     1801 ctgccggaac ctggacctct ttatgatgag cgaggaggtt gccctgcgtg cctttcagcg
     1861 aggcatcgag ggcgcggagg tcgccgtcgt ggagggggcg atgggactgt acgacggact
     1921 ggacctcgag ggcagcggga gcactgcaca gctggcgagg ctcctttccg cacccattct
     1981 gctggtcgtc gacgccaccc gtatgacccg aagtgttgca gcgctggtga ccggtttcca
     2041 gcggtttgag cctggggtca atgtggccgg cgtcgtgctg aacaacattg cccggcctcg
     2101 ccacgaggag atgctggtcc gctctatcca gcactactgc ggcatccccg ttctgggttg
     2161 catccccagg tctgaatccc tcgccatccc ggatcgacac cttggcctca tccccgtcgc
     2221 ggaggatcac gggcttacct ctattatcca aaatgctcga cggatcgcgg aagagcggct
     2281 agacctgggt cggatcctgg ctgtggccgg ctctgccccc gagttagcac ctcccatgct
     2341 tggggtggcc caatgccgga aacgtctcgt caggatggca gttcttttgg accgtgtgtt
     2401 cagcttctac tatcctgaga accttgaggc gctggaggcg gctggggcag tcctggagca
     2461 tgtggattct ctgtcggatc cctcgctccc cgatgtggac ggactgtacg ttggtggcgg
     2521 cttccctgaa ctgttcatgg agcagttgga ggcaaacgag tcgctgcgtc gggacattcg
     2581 cgctcggatc gatggcggcg ggacaaacat tggcgcagga ggtgcaacgc gtgcatctat
     2641 ctgcgttgat ctcgacggac ataccgcccc ggacaggctt cggcttgatg gcgccg
//
LOCUS       DATALS010000212         1864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_22409,
            whole genome shotgun sequence.
ACCESSION   DATALS010000212 DATALS010000000
VERSION     DATALS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22409"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..879
                     /locus_tag="VHS06_04675"
     CDS             121..879
                     /locus_tag="VHS06_04675"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX2987448.1"
                     /translation="MHSRWYGRDTGLTVRMFVTMFLLTAVYLGFLAALWYSGIDYVTL
                     AVIAVVLVGVQYYFSDRMVLWSTGARVVSPEEAPELHGAVERLVALADLPKPKVAIVD
                     TDVPNAFATGRNPENAVVAATAGLIRRLDPPELEAVLAHELTHVKNRDAMVITIASFL
                     STVAFFIMRSFMWGGFGGFGGYGRKGWSRRAKQPSLDAVDLSTRGEWMLALRLSRGDT
                     YCHAERSEASPRTRRDPSLSLRMTPATITVVEHQ"
     gene            1054..>1864
                     /locus_tag="VHS06_04680"
     CDS             1054..>1864
                     /locus_tag="VHS06_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2987449.1"
                     /translation="MKNWLKGFAAGIVAMALVAGAAAGYIHYVQPQMGSSALVAGASA
                     VVTRQSILDEDTVVSVYEKLSPTVVNITSKGIQVDTPLGQIPQQGAGSGVIIDDQGHI
                     LTNNHVVEGATELEVTLSNGDSVQGKLLGRDPGTDLAVIKIDVDKAKLTVAPLGDSNA
                     LKVGQLAIAIGNPFGLDRTVTVGVISSLGRTYASSTGSRPIRDMIQTDAAINPGNSGG
                     PLLNSKGEVIGINSVIESPVRGSVGIGFAIPINTAQRILPDLIAGKSITHPW"
BASE COUNT          378 a          572 c          569 g          345 t
ORIGIN      
        1 gggtgacaat cggaactgcc accgacttgt cggatgcacc acttagggct acaggaggca
       61 agggtgccga cagcctcccg accttgccgg ggccggcaat ggtagtaggg agcagcagcc
      121 atgcattcga gatggtacgg aagagatacc ggcctgacgg tgcgcatgtt cgtgacgatg
      181 ttcctcctga ccgcagtcta cctgggcttc ctcgccgccc tctggtactc tgggatcgac
      241 tacgtcacct tggctgtcat cgccgtcgtc ctggtgggag tgcagtatta cttctccgat
      301 aggatggtgc tctggtcgac cggagctcga gtggtgtcgc ccgaggaggc ccccgagctg
      361 cacggggctg tggagaggct ggtggccctg gccgaccttc cgaaaccgaa ggtggccata
      421 gtggatacgg acgtccccaa cgcattcgcg acggggcgta atccagagaa cgcagtcgtt
      481 gcggccaccg ccggactcat caggcggttg gatcctccag agctggaggc ggtgctcgcc
      541 cacgagctca cccacgtgaa gaaccgcgac gccatggtca tcacgatcgc gagcttcctc
      601 tccacggtgg ccttcttcat catgcgcagc ttcatgtggg gtggattcgg gggctttggc
      661 ggatacggtc gcaagggttg gtcacgccga gctaagcagc cttccctgga tgcagtcgac
      721 ctctccacga gaggagagtg gatgctagcc ctccgcctca gcagaggtga cacgtactgt
      781 catgccgagc gaagcgaagc atctcctcgt acccggagag atccttcgct atcgctcagg
      841 atgacaccgg ccacgatcac tgtggtcgag caccaatagc tgaaggctga gcgcggatag
      901 ctgattcaaa gatttaacag agtcctaaca actctgttgc gaccaactgc tagcatctac
      961 ctctagaaga gtgcagcgct gtagcggcac gcatggcgta ctccgatggc atctccggca
     1021 gtgacaccga cccggattct gggaggtact tcgatgaaga actggctgaa aggcttcgcc
     1081 gcggggatcg tggcaatggc gctggtcgcg ggggcagcag ccggctacat tcactacgtt
     1141 caaccccaaa tgggctcctc cgcgctggtg gcaggagctt cggcagtcgt caccaggcaa
     1201 agcatattgg acgaggacac tgtagtctcc gtctatgaga agctgagccc cacggtggtc
     1261 aacattacca gcaagggcat tcaggtcgac actcccctgg ggcaaatccc tcagcagggc
     1321 gcgggctcag gggtgatcat cgacgaccag ggccacattc tcaccaacaa tcacgtcgtg
     1381 gagggcgcga ctgaactgga ggtgaccctc tccaacggcg actcggtaca gggcaagttg
     1441 ctgggaaggg atcccggcac cgacctcgcc gtcatcaaga tcgatgtcga caaggccaag
     1501 ctcaccgtcg ccccattggg tgactccaat gcgctcaagg tgggacagtt ggccatagcc
     1561 atcggcaatc cattcgggct ggaccgcacg gtgactgtgg gcgtgatcag ctccctgggc
     1621 cgcacctatg cttcgagcac gggaagtcgc ccgatccgcg acatgatcca gaccgatgcc
     1681 gctattaatc ccggcaactc cggcggccca ctcctcaact ccaagggaga ggtgatcggc
     1741 atcaactcgg ttatcgagag cccggttcgt ggttccgtgg gcatcggctt cgccatcccc
     1801 atcaacacag cccagcgcat cctgcccgac ctgatcgcag gcaagagcat aacccacccc
     1861 tggc
//
LOCUS       DATALS010000213         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_100319, whole genome shotgun sequence.
ACCESSION   DATALS010000213 DATALS010000000
VERSION     DATALS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100319"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VHS06_04685"
     CDS             complement(<1..899)
                     /locus_tag="VHS06_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987450.1"
                     /translation="MNLRVRLFLAIIGPLVLVVSATTFFTARATSDSMLGQMEQDGLA
                     IAQMLAQSANFASRVPGRAEEVLANQMVVEARTMAELVAVAEKQAGMSPKEINAILND
                     IAAHTTLDEIWISDENGHVYLTNTGTDFTFSPNPQEQPQAYVFYTLLGQQDGTMIQEA
                     APRSLDSSVFKYVGVSGVDKPRIVQVGYRASILEQLSKDLNAQKLVDDLTGQGNVVNI
                     RLLDGYGRLVAGSAQPTRDTGQTISEDDRRMVDDAAASHQVRSALREGLLRVAAPVIS
                     QEGRTQMVALVYIATDSVQQAIR"
     gene            1212..>1975
                     /locus_tag="VHS06_04690"
     CDS             1212..>1975
                     /locus_tag="VHS06_04690"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX2987451.1"
                     /translation="MPTTPSGAVAWPQRVKWDLESGYRKTEGELLDLALLGTGGMTPL
                     PKRWLASLLVRHRGGLLLVDCGEGTQITLKMLGWGFKEISTILITHLHADHIAGLPGM
                     LLNIANSGRREPLLLYGPEELWRVVEGVRAIAPQLPYQLEWSELSSGDQIDLEGMEIS
                     CLAVEHSLPCLAYCLYVPRSRRFNPERASQLGIPMPLWGRLQAGQTVEFDGVRVSPEA
                     VLGPERRGIKLCYVTDTRPTPELPEFVQGADLLVCEG"
BASE COUNT          358 a          599 c          631 g          387 t
ORIGIN      
        1 tcccgaatag cctgctgcac gctgtcggtg gcgatgtaga ccagggcgac catctgggtc
       61 ctcccctcct ggctaatgac cggagctgcc accctcagga gaccttcccg cagcgccgac
      121 ctgacctggt gagaggcggc agcgtcgtcg accatccgcc ggtcgtcttc ggagatcgtc
      181 tggccggtat ccctcgtcgg ttgagcactg cctgccacca aacggccgta gccgtccagc
      241 aaccggatat tcaccacgtt cccctgccct gtgaggtcgt ccaccagttt ctgtgcattc
      301 aggtccttgg aaagctgctc caggatgctg gccctgtacc ctacctgcac gatccgcggc
      361 ttgtcgacgc cgcttactcc cacgtacttg aagacgctag agtctagcga gcgaggggcg
      421 gcctcctgga tcatggtgcc gtcctgctgt ccgagcaggg tgtagaagac gtatgcctgg
      481 ggctgctcct gggggttcgg gctgaaggtg aagtcggtcc ccgtgttggt gaggtacacg
      541 tgcccattct cgtcgctgat ccagatttcg tccaaagtgg tatgggcagc gatgtcattc
      601 aggattgcgt tgatctcttt cggagacatg ccggcctgct tctcggccac ggccaccaac
      661 tcggccatcg tacgagcctc taccaccatc tggttggcca gcacctcctc ggctcgccca
      721 ggcaccctcg aggcgaagtt ggcgctctgg gccagcatct gtgcgatggc caacccgtct
      781 tgctccatct gccccagcat agagtcggat gtggccctgg cagtgaagaa ggtcgtggcc
      841 gacacgacca ggaccagcgg tccgattatg gcgagaaaga gtctaaccct gagattcatt
      901 catattacct cgtcgatctc attgagcctc gttccatcgt tgcagaagat gaaacgaggc
      961 taccctatag ccgatatagc cgatcgagcc accgtctgca ccgtacgatc ggaacgaatg
     1021 tgggggactc cgagtggcga catgcacatg gagagatagg cgggacagtt ggtcatggac
     1081 gctgtggcga actcggatcg cccgtatcac tgcattgaga tatctatata gagatatcgg
     1141 caggcgatgt ccatgcctga agtcccacct ggccggctaa cgaccggctt cagcctttgg
     1201 acgagggctc cgtgccgacg acgccgagcg gtgcagttgc ctggccgcaa agggtaaaat
     1261 gggatctgga atcgggctac cggaagacgg agggcgagtt gctggatctg gctttgctgg
     1321 ggactggagg gatgacgccg ctgccaaaac gatggctggc gagcctgctg gtccgacatc
     1381 ggggcggtct gctcctcgtg gactgcggtg aggggacgca gataacgctg aagatgctgg
     1441 gttggggctt caaggagatt tccaccatcc tgatcaccca cctccacgcc gaccacatcg
     1501 ccggtcttcc cgggatgctg ctcaacatcg ccaactccgg ccgcagggag ccgctgctcc
     1561 tctacggtcc agaagagctg tggcgggtgg tcgagggggt gagggccatt gccccacagc
     1621 tcccatatca gctggagtgg agcgagctct caagcgggga tcagatcgat ctagagggga
     1681 tggagatctc ctgcctcgcc gtggagcaca gcctgccctg cctggcctac tgcctgtacg
     1741 tgcccaggag caggcgcttc aacccggagc gggcctcaca gctgggtatc ccgatgccgc
     1801 tatgggggag gctccaggcg ggacagacgg tggagttcga tggggtgagg gtctcacccg
     1861 aggcggtgct ggggcccgag cggcgaggga tcaagctgtg ctacgtcacc gacacccgcc
     1921 ccaccccgga gcttcccgag ttcgtgcagg gcgcggacct gctggtctgc gaagg
//
LOCUS       DATALS010000214         4982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_246702, whole genome shotgun sequence.
ACCESSION   DATALS010000214 DATALS010000000
VERSION     DATALS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246702"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VHS06_04695"
     CDS             <1..969
                     /locus_tag="VHS06_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987452.1"
                     /translation="VSWLVMAEEVAVQDLREGRFQRSMAAMAAFSAVVSGWEAYSQHL
                     RGAFSNWLMWTPVLLTPPMVIAALASPVAGTWGRLLLLAVSLVSLLDGVVGFYFHIRG
                     IGRLPGGFRIGQYNVVMGPPIFAPLLLCSVGILGLLATLLRPERLRPQSRLAEMVDDK
                     LPILLSGRRPVPLGRSPRMRWARTIARRVARGRFQGGMALIAAFLGLLSAGEVYFEHL
                     RGSFNQRVMWTPIWLTLPTVAAAVGAAQGRAPAAHRALTVLSVATFLDGLVGFGFHLR
                     GIRRMPGGFSNLQFNVTLGPPLFAPLLFTAVGLLGFISTILRRKEG"
     gene            975..1589
                     /locus_tag="VHS06_04700"
     CDS             975..1589
                     /locus_tag="VHS06_04700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX2987453.1"
                     /translation="MSERYPGYRVLEQRGHWDDVTRRVVMDRVHNVPAFSHFDDHQRA
                     TLEALCERVLPQGDRPISRRIPIAPWIDRNAGNDVVEGFRFDDMPPIAQAWEWGLEGV
                     DQTARALFDSPFVDLDGSRQDQVLESIRTGSPPGDVWRRMPAERWWRQVALRQICGVY
                     YAHPYAWDEIGFGGPAYPRGYFALNHGAPEPWEVHEASRGDGRR"
     gene            1642..2490
                     /locus_tag="VHS06_04705"
                     /pseudo
     CDS             1642..2490
                     /locus_tag="VHS06_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
     gene            2888..3259
                     /locus_tag="VHS06_04710"
     CDS             2888..3259
                     /locus_tag="VHS06_04710"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX2987454.1"
                     /translation="MPYVHFNLFENDRKMMRAGVARAEEVLRAAGARETHWVPRYAHL
                     VGTCRMGFTPSDSVVDRWCRSWDVPNLFICDGSLLPTQGSANPALTISALAARTADWI
                     REVAPKGELRRAATVEMGASL"
     gene            complement(3561..3887)
                     /locus_tag="VHS06_04715"
     CDS             complement(3561..3887)
                     /locus_tag="VHS06_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987455.1"
                     /translation="MNEYREPGFIDYDKLDTPLSRADLALLAIVLGFFAAIMATSLGN
                     TSGLPDLLLRGFVTAVVTVGAIASLRGTILWGSTTEDAKRAIESARLSQRSHEIDLFH
                     TQSRAI"
     gene            4048..4209
                     /locus_tag="VHS06_04720"
     CDS             4048..4209
                     /locus_tag="VHS06_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987456.1"
                     /translation="MPRNSDARLTDIHIGSHNAAGMAEESTELDPYTISFGRNMVEAI
                     AANSALAGR"
     gene            4222..>4982
                     /locus_tag="VHS06_04725"
     CDS             4222..>4982
                     /locus_tag="VHS06_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987457.1"
                     /translation="MIERKTAWNRGDGLSLLILFLGISAFHIRGISPGRTFLAVDLSN
                     NNLPWLRGSPQPLQNWLISDPVYQTYPFLVQAVESFQHGSWPLWNPHILIGHPSFADP
                     LAQSFYPLFILLGCLLGPARGLAIGLWLHALMASFLTYGLLRTVGCGRIGALVGGFTY
                     TLGGYMVNWFETAFWISTLTWLPGVLWCFELARRKRALHFLAAGGVAFSLALLGGQLA
                     FAFTFALFFCLYAAGRWVESMLLARSGRWDIAGAAA"
BASE COUNT          804 a         1493 c         1697 g          988 t
ORIGIN      
        1 gtaagctggc tcgtgatggc cgaagaggtc gccgtccagg acctgaggga gggccgcttt
       61 cagcgcagta tggcggccat ggctgccttt tccgccgttg tcagcggttg ggaggcatac
      121 agccagcacc ttcggggggc attctccaac tggctcatgt ggacgcctgt gctgctcact
      181 ccccccatgg tgatcgccgc gctggcttct cctgtggccg ggacctgggg gcgtctcctc
      241 ctgctggcgg tttctctagt atcactgttg gatggggtgg tggggttcta cttccacatc
      301 cgtggtatag ggcggttgcc cggcgggttc aggatcgggc agtacaacgt ggtgatgggc
      361 ccccccatct tcgcaccgct gttgttgtgt agcgtcggga tcctggggct gctggccacg
      421 ttgctgcgcc ccgaacggct gaggccccag agcaggctgg cggagatggt ggacgacaag
      481 ttgcctatcc tactctctgg ccgccggcct gttcctctgg ggcgctctcc acgcatgcga
      541 tgggcgagaa ccatcgcgag gagggtggcg cgaggccgtt tccagggcgg gatggcgctc
      601 atcgccgctt tcctggggct gctttcagcg ggagaggtgt acttcgagca tctgcgcgga
      661 agcttcaatc agcgggtgat gtggactcca atctggctga ccctgcccac cgtggctgcg
      721 gccgttgggg cggcacaggg gagggcaccc gccgctcatc gagcgctgac ggtcctctcc
      781 gtggcgacct tcctggacgg gttggtgggg ttcggcttcc acctgcgggg catacgcagg
      841 atgcccggcg gcttctcgaa tctgcagttc aacgtcaccc ttgggccgcc actctttgcc
      901 ccactcctct tcaccgcggt ggggctgttg ggtttcatct ccaccatcct gagaagaaag
      961 gagggctgac ggcgttgagc gagcggtatc cgggttatcg ggttctggag cagagggggc
     1021 actgggacga cgtcactcgc cgcgtcgtca tggaccgcgt ccacaacgta cccgccttta
     1081 gccatttcga cgaccaccag cgggctacgc tggaagcttt gtgcgagcgg gtgctgccgc
     1141 agggggaccg tcctatctcc aggcgcatcc ccattgcccc ctggatagac cggaacgctg
     1201 gaaacgacgt ggtcgaggga ttccgcttcg acgacatgcc acccatcgcc caagcatggg
     1261 agtggggact ggagggggtg gaccagacgg ctcgggccct cttcgacagc ccgtttgtcg
     1321 atctggatgg atccaggcag gatcaggtac tggagtcaat ccgcaccggc agccctcccg
     1381 gggatgtgtg gcggcggatg ccggccgagc ggtggtggag acaggtggcg ctacgccaga
     1441 tctgcggcgt gtattacgcc catccctacg cctgggacga gatcgggttt ggcggtccgg
     1501 cctaccctcg gggctacttc gccctgaacc acggcgcccc cgagccgtgg gaggttcacg
     1561 aggcgtctcg cggggacggg aggcgctgat atggcgaagc tgaacgtgtt cctttcccct
     1621 aactggttgc tggacgacga gatgaaggag taccggcccg gggaggaggt ggatttctgc
     1681 atcgtcgggt cgggggccgg aggcggcgtt cttgcccagc ggctggctcg ctacggtttc
     1741 tccgtggtgg tgctggaggc tggcccgtgg cacgacgccg agcgggatat ggtctccgac
     1801 gaggctggat ctcagcggct ctattggaat gaccttcgga tcaccggggg gaccgatccg
     1861 ttggagttgg gggccaacaa ctccggccgc ggcgtcggcg gaggcacgat ccactacgcc
     1921 ggcttctgcc ccaggtttca tccctccgac tttcgggtgc ggagtctgga cggcgtggca
     1981 gccgattggc ccatctccta cgaggagttg gagccctact acgagagcat ggagcgggag
     2041 taccccatct ccggtcccgc ctattacccc tggggcaagc cccacggcta cccctacgga
     2101 ccgctgcagg cgggcaccgc gggccagatg ctgatcgagg ggtgtgtgaa gttggggatc
     2161 cccgtcgttg ccggcgggcc ggtggcgatc cctgccggcg ccgtggacaa gcggccccat
     2221 tgcatcatgc gcggcttctg cctgctcggc tgcaaggtgg gtgcgaagag cagcatcatg
     2281 gtcagccaca tccccgacgc catccgccac ggcgccgaga ttcgcactcg ctccatggcc
     2341 tacgagatcc ccatcgacga cgagggcagg gcggtcggag tacgctacta ccgcagtgac
     2401 gggaacggca acctgaaaga ggaggagcag cgcgcgcggt cggtcgtggt ggcgggctat
     2461 gcgatcgaga cgcccaggct gcttctgaac tcaagctcgg ggcgctttcc acaggggctg
     2521 gccaactcca gcggtctggt cggcaagtac ctgatggcgc aggcggggcc ggtggtgtgg
     2581 ggccgcttcg atgagttgat ccgtcagtac aaggctccgc cggcctgcgc cctcacggag
     2641 gagttctacg agaccgatcc ccggaacgac ttcgtccgtg gctatgccct gcagacggtg
     2701 gctccattgc caatctccat gtcccacctg ctggtggacg aggatggcag cttcggaatg
     2761 ccgttgaggc gggcgatgga ggagtacaac cattacgcct gcatcggcgt actgggggga
     2821 gatcctcccg gatgtccgga actccgtctc tattcacccc actctcaagg accggtacgg
     2881 catccccatt ccctacgttc atttcaacct gttcgagaac gacaggaaga tgatgcgggc
     2941 gggagtggcc agggcggagg aggtgctgcg ggccgcgggg gcgagggaga cccactgggt
     3001 tccgcgctac gcccaccttg ttggaacctg ccggatgggc ttcaccccat ccgattcggt
     3061 ggtagacagg tggtgtcgga gttgggacgt tccaaacctc ttcatttgcg acgggagcct
     3121 gctgcctact cagggctccg ccaatcctgc cctcacaatc tctgcgctgg ctgcgcggac
     3181 cgcggattgg atcagggagg tggccccaaa gggcgagttg aggcgagcgg cgaccgtgga
     3241 gatgggggcg tccctgtgaa cgcgaaggat catcggctgc tcccacctgg acggaccgtc
     3301 tcccgctggc gggagttctg ggcagccctg gtttggatag tcggctcgtg gatccgttca
     3361 gcaggcagcg aggcagggtg gcagcagaag cgatgacggg ctcggcgatg tatggtcgcg
     3421 atcgaccgaa cgtctgagag tcttgtgcat acggcaaggc cggacaccca tcgtgcccgg
     3481 cctcgtggtt gaagggattc aggccgctcg tggtagagcg gcggccttag gcctgtatat
     3541 gttaagcggt agcaggaccg ctaaattgcc cgcgactggg tgtgaaagag atcgatttcg
     3601 tggctgcgct gcgacagccg ggcggactcg atcgcccgct tggcatcctc ggtagtgctt
     3661 ccccacagga tcgtaccccg cagcgaagcg atggcgccga ccgtcacgac ggcggtgacg
     3721 aagccacgga ggaggaggtc aggcagcccg ctggtgttgc cgaggctggt ggccatgatg
     3781 gcggcgaaga aacctagaac tatggcgagg agcgcgaggt ctgcgcggga aagtggggta
     3841 tcgagcttat cgtaatctat gaaaccgggc tcacggtatt cgttcattgt atcctccctg
     3901 gacccttcaa cccatctctg ttgcaggctg atgatgccac agccgaaacg ataagtccaa
     3961 tgttggttga agcgatatat gattacatcc aaggcatgga gcggggggtg gtcgctgccc
     4021 tcgccgacta agcgggcagg gctgcggatg ccgcggaaca gtgatgctcg attgaccgac
     4081 atacatatag gaagccataa tgcggcgggg atggcagagg agtctaccga actggaccct
     4141 tacacgatta gcttcgggcg caacatggtg gaggctattg ccgccaactc cgcgttggct
     4201 ggacgctgaa ctgtcgggga aatgattgag cggaagacgg cctggaatag gggagacgga
     4261 ctgtcgctgc tgatcctgtt cctggggatt tcggcgttcc atatccgcgg catctccccg
     4321 ggccggacct ttctggcggt cgacctatcg aacaacaacc ttccctggct ccgcggtagt
     4381 ccgcagccgc tacagaattg gctcatctcc gatccggttt accagaccta ccctttcctc
     4441 gtccaggccg tcgagtcgtt ccagcacggt agctggcccc tctggaaccc ccacatcctg
     4501 ataggccacc catcctttgc cgacccactg gctcagagct tctatcccct cttcattcta
     4561 ctgggttgcc tgctgggccc cgctcgaggt ctcgccatcg gcctctggct gcacgccctg
     4621 atggcgtcgt tcctgaccta tgggctgctg cgaactgtcg gctgcggccg tatcggagca
     4681 cttgtagggg gcttcaccta cacactgggc ggctatatgg tgaactggtt cgagacggcc
     4741 ttctggatct ccaccctgac ctggctgccg ggagtgctgt ggtgcttcga gttggcgcgc
     4801 cggaagcgcg ctctccattt cctggcggcg ggaggcgtag ccttctccct tgcactgttg
     4861 ggtggccagt tggcctttgc cttcaccttt gcgctcttct tctgcctgta cgccgcgggc
     4921 agatgggtgg agtcgatgct actggctcgg tctggacgat gggacatcgc cggggcagcg
     4981 gc
//
LOCUS       DATALS010000215         5086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_204452, whole genome shotgun sequence.
ACCESSION   DATALS010000215 DATALS010000000
VERSION     DATALS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204452"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VHS06_04730"
     CDS             <1..1182
                     /locus_tag="VHS06_04730"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2987458.1"
                     /translation="TLNAVRDQIRSAIDGKDYERAQTLVEDTLRRYPEDHETVGLLGQ
                     LHLECGRWDEAEPLFRSLLRVDPESILARSGLALIAEARGDLFDALDQFTLSLEIHPN
                     NQQLAGEVRRLLGQSKRPRLADPRYSKHAVARRLLADGMAGDAVPLFEASLEKALDPT
                     VVALGMAEALWLAGRRREAGDVAAEILAANPVCLKALALRAAAAAAEGESDLSPLLEK
                     AWRLDPDNGVVRRVFESAGLVAPDGDPSPELPDQVEHSPEGEVPELAGRPEEPDGFEP
                     QDLPCAEGDGEWVRDGGVAEPQKPSDREGTEPEEDGKSHLATGLAMQAQHSYDLAAVE
                     FRKALKLDPRLAPEVRIAAQSMVEESPGDPRLRWLAGDALAAEGQLRSAMDQYLKVLE
                     G"
     gene            1266..1715
                     /gene="ndk"
                     /locus_tag="VHS06_04735"
     CDS             1266..1715
                     /gene="ndk"
                     /locus_tag="VHS06_04735"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX2987459.1"
                     /translation="MERTLVIVKPDGVQRGLIGEVLKRLETRGLKMVGLKMMAVSPEL
                     AQRHYAVHQGKPFFEGLIRYITSSPVVVAVFEGKDAIAAVRSTVGATNPVQATPGTIR
                     GDFGMEIGRNLVHASDAPDTAKAEIALWFKEEELVSWSRDTDRWISE"
     gene            complement(1917..2264)
                     /gene="rsfS"
                     /locus_tag="VHS06_04740"
     CDS             complement(1917..2264)
                     /gene="rsfS"
                     /locus_tag="VHS06_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX2987460.1"
                     /translation="MESQALARKIVDIASDKQAVDIVLLDLRDVSLIADYFVICNGTS
                     DRQIKAIVDDIREILSREEKVKPLQIEGRPDSGWVLLDYGSVVVHVFSPELREYYRLE
                     KIWPQAVPVLRMA"
     gene            complement(2320..2706)
                     /locus_tag="VHS06_04745"
     CDS             complement(2320..2706)
                     /locus_tag="VHS06_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987461.1"
                     /translation="MAKKVLIVEDEPEVQEVERMVVEDLLGCEVTLASSGEEALELVS
                     GVAPDLVMLDLVLPGIDGFTVAERMRTQPELDRTRILALSGLTRPEDRERARAAGCND
                     VLDKPFDLDDLMKKIEGLVGTCTPTN"
     gene            complement(2829..4226)
                     /locus_tag="VHS06_04750"
     CDS             complement(2829..4226)
                     /locus_tag="VHS06_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2987462.1"
                     /translation="MVRVARRPKVSKRRHVQAKQRRAAQGETQKLIDQGIIRLVIQVI
                     QQALREEVTRLLGRAKGQRRDLSDRMVVEARCNRCGSQYRADFYRAGFYLRGLLSLAA
                     WGQIGVPRVSCRCGGMVDFEFDHLVPYGRLWFDMEERARELAAMCVSLRDGVRVLSWA
                     SGQPVSIATLNGLVNRTSDLAAAFHQGMLERIPAVVMLDGVWLKVLEPTGEEFLDKQG
                     RKRDRLKTRKFPLLVAYGADPTSGERWVLDWQKGREEDLESWRTLLMRLQERGISETR
                     GLRLFIHDGSLGLERALELVYFGDGVERQRCIFHKLQNVKRDVLGDEGMSPKERSQRR
                     KEVLSDAAEVYKGKDEQETRQRLERFRVKWQDKEPRAVETLERGFEKTLVYLKVAEKA
                     RRREEEWRVECLRTTSPLERVQRAFRQKARQAAIFHSHKGVDAAIQLVIAHRHLADNS
                     TQFWGHLLEEALLAA"
     gene            complement(4423..5046)
                     /locus_tag="VHS06_04755"
     CDS             complement(4423..5046)
                     /locus_tag="VHS06_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX2987463.1"
                     /translation="MAGNNNRTTTLITDPPIAQLLFNDTRLSWLWLIVRLYVGYSWLT
                     SGLGKVTNPAWTVTGEALRGFWTNAVQIPAQGNPQITFGWYRDFITFLLNGGHYVWFA
                     KLIVFGELAIGTALILGAFTGIAAFFGAFMNWNFLLAGTSSVNPILFTLSILLMLAWK
                     TAGYIGVDRWLLPRLGTPWGPNIVTRREVEEAIPHEDRDRERKRPAG"
BASE COUNT          977 a         1603 c         1497 g         1009 t
ORIGIN      
        1 actctcaatg ctgtccggga tcagatcagg tctgccattg acggaaagga ctacgaacgg
       61 gcgcagacgc tggtcgagga cacgcttcgc cgctaccctg aggaccatga gaccgtgggg
      121 ctgttgggac agctccacct ggaatgtggg cgctgggacg aggctgagcc actgttccgc
      181 tctctcctgc gagtggaccc ggagagtatc ctcgcccgtt ccggcctggc cctcattgcg
      241 gaggccaggg gcgacctgtt tgatgccctg gatcagttca ccctgagcct ggagatccat
      301 cccaacaacc aacagttggc gggagaggtc cgcaggttgc tcgggcagtc caagcgtccc
      361 aggttggccg atccacgtta ctccaaacat gctgttgccc gccggctgct ggccgatggg
      421 atggctggag atgccgtgcc cctcttcgag gcatcgctgg agaaagcgct ggatccgacg
      481 gttgtggcgt tggggatggc ggaggcgctg tggctcgccg ggcgaaggcg tgaggccggg
      541 gatgtcgccg cagaaatcct ggcggccaac cccgtctgtc tcaaggcact cgcacttcgc
      601 gccgccgctg ctgccgccga gggcgaatcc gacctgtctc cgctgctgga gaaggcttgg
      661 agactcgacc cggacaatgg ggtggtgagg agggtcttcg agtctgccgg ccttgtcgct
      721 ccggacggcg acccatcgcc cgaattgccc gatcaggttg agcattcgcc agagggtgag
      781 gtgccagagc tggctggccg gcctgaggaa ccggatggct tcgagcccca ggacctgccc
      841 tgtgctgagg gggatgggga gtgggtcagg gatggcggag ttgcggaacc ccagaagcca
      901 tctgatcggg agggtacgga gcccgaggag gatgggaagt cccatttggc tacgggcctt
      961 gccatgcagg cccagcactc ctacgacctg gctgcggtgg agttccgcaa ggccctgaag
     1021 ctggatccga ggcttgcacc ggaggttcgg atcgctgccc agagcatggt cgaggagtcg
     1081 ccgggcgacc cgcggttgcg ttggctggca ggggatgccc ttgctgcgga gggacagctg
     1141 cggagtgcaa tggatcagta tctgaaggtg ctggaaggtt gactcgccta ctagcgcaga
     1201 tgtgacggtg acgcgtacct tttggacgca ccacatagaa tttgttgata gagacaggga
     1261 gcaagatgga gagaacactc gtaatcgtga agcctgatgg agtgcagcgc ggcctcatag
     1321 gcgaggtcct gaagcggcta gagacccggg gcctcaagat ggtcggcctg aagatgatgg
     1381 cggtgagtcc agagctagcg cagcgccatt acgccgttca ccaggggaag cccttctttg
     1441 aaggcttgat ccgctacatc acttcgtcgc cggtggtggt ggcggtgttc gagggcaagg
     1501 acgctatcgc cgcggtgcga tcgactgtgg gagctaccaa cccggtacag gctacgccgg
     1561 gcaccatccg aggcgatttc gggatggaga tcgggcgcaa cctagtgcac gcctccgacg
     1621 ctccagatac ggcgaaggcg gagattgccc tctggttcaa ggaagaggaa ctggtaagct
     1681 ggagcagaga cacggatagg tggatctcgg agtaacgacc ttacagccat cggcagtcag
     1741 ctatcagcac ccggaagctg gctgctgata gctgactgcc gatggcttct aagcacgtag
     1801 ccataagtcc gccggtgcaa ggcgggtcca gggcactcaa cgcgagcgag cacagcgttg
     1861 aggcctggcg gggtctgggg tgtccccaga aaaccatatt acttttgatc acatgcttag
     1921 gccatgcgca gcactgggac agcttggggc cagatcttct ccagacgata gtactcgcga
     1981 agctcgggtg agaagacgtg caccacaacg ctgccatagt ccagaaggac ccagccggag
     2041 tcgggccttc cctcgatctg gagcggcttc accttctctt cccgagagag gatctccctg
     2101 atgtcgtcga cgatggcctt gatctgccga tcgctcgtgc cgttgcagat aacaaagtag
     2161 tccgcaatga gtgagacatc ccggagatcg agcagcacaa tgtcgacggc ctgcttgtcg
     2221 gaagctatgt cgacgatctt tctggcgagg gcttgggatt ccagtcctgt gaacctcctc
     2281 ttcttgcctc agcctctgta tggcggagga ttataccatc tagttggttg gtgtgcaggt
     2341 gccgaccagc ccttcgatct tcttcatgag gtcgtccagg tcgaagggct tgtccaggac
     2401 atcgttgcac ccagcagccc tggccctctc gcggtcctcc ggtcgagtca gcccgctcag
     2461 ggccaggatg cgggttctat ccaattcggg ctgtgtgcgc atccgttctg ccacggtgaa
     2521 gccgtcgatg ccgggaagca ccaggtcgag catcaccagg tcgggagcca ctcccgatac
     2581 caactctaga gcctcctccc cactggaggc cagggtgacc tcgcaaccca gaaggtcctc
     2641 cacgaccatc cgctcaacct cttggacctc tggttcatcc tcaactatca gcaccttctt
     2701 cgccaaggaa tacgcctccg cttagctgac ttgccacagc ccaagccagg gtctgcagga
     2761 atcgaaccag cgaaagggct atgccgccgg tggtaacgta cgcgggattg cggaaaagtc
     2821 gttgggactc aagcagcaag gagcgcctcc tccaggaggt gaccccagaa ctgggtggag
     2881 ttatcggcca ggtggcggtg ggcgatgacc agctggatcg cggcgtctac gcccttgtgg
     2941 gagtgaaaga tggcagcctg acgggctttc tgcctgaagg ctcgctgtac tcgctccaaa
     3001 gggctcgtgg ttcgcagaca ctccaccctc cactcttcct ctcgccgccg ggccttctcg
     3061 gccaccttta ggtagaccag ggtcttctcg aagccccgct ccagggtttc cacggccctt
     3121 ggctctttgt cctgccactt tacccggaac ctttccaacc gctgccgggt ctcctgctcg
     3181 tccttgccct tgtacacctc tgcggcatcc gagaggacct ctttgcgccg ctgactccgc
     3241 tccttcgggc tcatcccctc atctcccaac acatcccgct tcacgttttg gagtttgtgg
     3301 aatatgcagc gttgacgctc caccccatcc ccgaaataga ccaactccag ggctctctcc
     3361 agtccgaggg agccatcatg aataaacagc ctaagccctc gcgtctcgga aatccccctc
     3421 tcctgaagcc tcatcagcag ggtacgccaa ctctccaggt cctcttccct acccttctgc
     3481 cagtcgagca cccaccgctc tccactcgtc ggatccgctc cgtaggccac cagcaatggg
     3541 aacttcctcg tcttcagccg atctcgtttc cgtccctgct tgtccaggaa ctcttcgccg
     3601 gtaggttcca gcaccttcag ccacaccccg tccagcatca ccaccgccgg aatgcgctcc
     3661 agcattccct gatggaaagc cgctgccaga tcggaagttc tgttgaccag cccgttcagg
     3721 gtcgcaatgg agaccggctg cccgctagcc caggagagga ctcggacccc gtctctaagg
     3781 gagacgcaca tagccgccaa ttccctcgcc cgctcctcca tgtcgaacca cagccgaccg
     3841 tagggcacaa gatggtcgaa ctcgaagtcc accatccccc cgcaacgaca actcaccctc
     3901 ggtaccccga tctgccccca ggccgccaag gagaggagtc ccctaaggta gaaacccgcc
     3961 cgatagaaat cggcacgata ctgactgccg cagcggttgc agcgagcctc caccaccatc
     4021 ctgtctgaca gatctcgccg ttgacccttg gctcgaccca acagccgagt cacttcctcc
     4081 cgaagagcct gctggattac ctggatcacc agccggatga tcccttgatc gatcagcttc
     4141 tgggtctctc cctgcgctgc tcgacgctgc ttagcctgaa catgccgcct cttggaaacc
     4201 ttcggccgcc gtgctactct caccattgag cccagcctcc ttcctatcgc ttgttcctgc
     4261 aaacgaaacg atagtcaggt agctgggctc atacaatagc taagaccgct ttaagccggc
     4321 ctattcccgc tcgtttccct aacaacgaga gtcacctgct ccagaccact gccctctccc
     4381 cccggtacac cccatctccc tatttccgca attgttcata cactaccccg ccggtctctt
     4441 tctctctctg tccctgtcct catggggtat cgcctcctct acctcccgac gagtcacgat
     4501 gtttggcccc cagggggtgc caagtcgcgg tagcagccac cggtcgacgc cgatgtaccc
     4561 tgccgtcttc caggccagca tcaacagtat cgacagcgtg aagaggatag ggttgacgct
     4621 ggaagtaccc gccagcagga agttccagtt catgaatgcg ccgaagaatg cagctatgcc
     4681 ggtgaaggcg cccaggatca gggccgtgcc tatcgctaac tctccgaaga cgatcagctt
     4741 ggcgaaccag acgtagtggc ctccattcag caggaaggta atgaagtccc tataccagcc
     4801 gaaggtaatc tgcggattgc cctgggcagg gatctggacg gcattggtcc agaagccccg
     4861 cagggcctcc cctgttaccg tccaggctgg attggtgacc ttgcccagac cagaggtgag
     4921 ccaggagtag cccacgtaga gtcggacaat cagccacagc caggacagtc gcgtgtcgtt
     4981 gaacagcagc tgggcgatgg gtggatcggt tatcagggtt gtcgtacgat tgttgttgcc
     5041 agccatggga ccaactcctt tgtcagagta gggcttgcgc ggtctc
//
LOCUS       DATALS010000216         4247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_247020, whole genome shotgun sequence.
ACCESSION   DATALS010000216 DATALS010000000
VERSION     DATALS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247020"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..100
                     /locus_tag="VHS06_04760"
                     /pseudo
     CDS             11..100
                     /locus_tag="VHS06_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            234..542
                     /locus_tag="VHS06_04765"
     CDS             234..542
                     /locus_tag="VHS06_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2987464.1"
                     /translation="MEEAIDEDGHRVVLVEIVQITEKESNTMADELRMARDGLLRKVQ
                     IEDGADFLRDGGKVLSQALMELEVSQQLGVERYERSPERMGQRNGYWDRTWDTRVGIS
                     "
     gene            complement(670..1728)
                     /locus_tag="VHS06_04770"
     CDS             complement(670..1728)
                     /locus_tag="VHS06_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX2987465.1"
                     /translation="MEDTGRRFDPAKLASFCTTVLVKVGVPAENARIVADSLLCANLR
                     GVDTHGVTRLPIYVERLQQGLTNGNTEGAVVAESETTAVYDGQDGLGQVIGTKAMQLA
                     IKKAESAGVGMVTARNSTHYGTAAYYAMMALSHDMIGLSVTNSPSLMVPWGGKRPFLG
                     SNPLAIAVPAGEEPPFIFDMATSVVARGAIILAAKKGDPIPLTWAVNSEGEPTSNAKE
                     ANEGGGLLPLGGHKGFGLAMAIEVLVATLAGGPFGPHIGELYNNPTRPQGVGHFFTAM
                     RIDRFRPVAEFKANMDAMIREVKLQPLAKGFDKIMVAGEPEANTERDRREKGVPLSDA
                     VVKDLTKLGASTGVRFES"
     gene            complement(1807..2538)
                     /locus_tag="VHS06_04775"
     CDS             complement(1807..2538)
                     /locus_tag="VHS06_04775"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2987466.1"
                     /translation="MLAKDSPLTLWRQVKIELRDKALKELAPGDRIPTEPELCAQFGV
                     SRITVRQAVSSLVREGILATQRGRGTYVLPPKIASRLDSSTSSLEGKAAKVMLYSFDR
                     ISADEWQAGKLGVPKGEELYRIRRVRLVEDEPLAYVTLFLLPRLFPEFTRPELESESF
                     SQLLICKAGLTPEKGEETIECIQADSFRSNILRVPSGSPLLVVETRAFLPSGEVMGFF
                     RAYHRADRTRLTREASGSQVLATIW"
     gene            complement(2561..2857)
                     /locus_tag="VHS06_04780"
     CDS             complement(2561..2857)
                     /locus_tag="VHS06_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987467.1"
                     /translation="MTLQLQAGSELDRLIHEKVVKGEGEAPAYSTDIAVAWKLWQGLP
                     RPKRLHVDAARVHHCMCGGTDESADGVFKPDVWETADKMALAICRAALKMVEKS"
     gene            complement(2895..3299)
                     /locus_tag="VHS06_04785"
     CDS             complement(2895..3299)
                     /locus_tag="VHS06_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX2987468.1"
                     /translation="MALDLASAEKIAQKGLERAREMGLKVSFAVLDESGNLVYVARMD
                     GAPFFSPNIAMGKAWVSSAWRISSGDIEKKAQAAPYFYESVVTASGGRGVIRQGALPI
                     VIDGKVVGAAGVSGGTSVEDEEIVRAGLQAIA"
     gene            complement(3388..>4247)
                     /locus_tag="VHS06_04790"
     CDS             complement(3388..>4247)
                     /locus_tag="VHS06_04790"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2987469.1"
                     /translation="SMARRLLSGGYQVTVWNRSPDKVRPLVELGARAAASPKEVAAAS
                     DIVITMVANSHASEEVACGPDGVLEGAHPGMVLVDMGSIDPETSREISRKAEALGVSM
                     LDAPVTGNGSVAAEGNLGIMIGGPKEALERCLPVFERLGTKIVYAGPTGSGATLKIIN
                     NLVLNVAIEAACEALVLATKAGIDPALVIDITSVGGARTFAMQSRGPRILARDFEARS
                     SINTQHKDLTNAVRLAEQLQVPLPVTSGVREIFQAARAKGLGSLVTAAVVTVLEGLAD
                     VTVKSSVAS"
BASE COUNT          782 a         1404 c         1230 g          831 t
ORIGIN      
        1 cgacaaggcg ctgaaggaac tggcgccggg tgacaggatt ccaactgagc cggagctgtg
       61 cgctcaattc ggcgtaagcc gtattaccgt gcggcacgcg aatctatgtt gtgaacgtcc
      121 tcgtgccctc ggctagagcg cgctcggcgt cctagagaca gccttgtttg tcaaacggga
      181 gtggcccact tctccttcgc atcgccccga tagagtcgcc gtgagtgtga gaaatggaag
      241 aggccattga cgaagacggc catcgtgtgg ttctcgtcga gattgtccag atcacagaaa
      301 aggagagcaa caccatggcc gatgagctta ggatggcacg agacgggctt cttcgcaagg
      361 tccagataga ggacggtgca gacttcctgc gtgacggggg gaaagtgctc agccaggcgc
      421 tgatggagtt ggaggtcagt cagcagctgg gagtcgagag gtacgagcga agccctgaac
      481 ggatgggaca gcgcaacggg tattgggatc gaacctggga cacgagagtt gggatcagct
      541 aagtggcgca tccggcaagt ccatgacagt tccaattgtc accctgcgcg gagcgaaggg
      601 tctccagtcg cgaggagaag cttcgcttcg ctcagcttga cagtgccgtg aacttgccgg
      661 acaagccgct tagctctcga accgcacgcc cgtgctcgcg ccgagcttcg tgaggtcctt
      721 gacgaccgca tcgctcagag gcacgccctt ctccctgcga tcccgctcgg tgttggcctc
      781 gggctcgccc gccaccatga tcttgtcgaa gcccttagcc agcggctgca acttcacctc
      841 gcggatcatc gcgtccatgt tggccttgaa ctcggccact gggcggaacc ggtcgatccg
      901 catcgccgtg aagaagtgcc ccacgccctg cggcctcgtc gggttgttgt acaactcgcc
      961 gatgtggggg ccgaaaggac cgcccgcgag cgtcgcaacc agcacctcga tcgccatggc
     1021 gaggccgaag cccttgtgcc cgccgagggg cagcaatccg ccgccctcgt tcgcttcctt
     1081 cgcgttgctc gtcggctcac cctcggagtt cacagcccag gtcagcggga tcgggtcgcc
     1141 cttcttcgcc gcgaggatga tcgcacctcg cgccacgacg ctggttgcca tgtcgaagat
     1201 gaacggcggc tcctcgcccg ccggcaccgc tatcgccagc ggattgctcc cgaggaacgg
     1261 tcgcttccca ccccatggca ccatcagcga gggtgagttg gtcactgata ggccgatcat
     1321 gtcgtgtgag agcgccatca tggcgtaata ggcggcggtc ccatagtgcg tcgagttccg
     1381 cgccgtcacc atcccgacgc ccgcgctctc ggccttcttg atcgcgagtt gcatcgcctt
     1441 cgtgccgatc acttggccga ggccgtcttg accgtcgtac acagcggtag tctcgctctc
     1501 ggcgacgacc gcgccctcgg tgttgccgtt ggtcagcccc tgctggagcc gctcaacgta
     1561 aatcggcagc ctggtcacgc cgtgagtgtc cactccgcga aggtttgcgc atagtagtga
     1621 atcggccacg atcctagcgt tctcggcggg cactcccacc ttcaccagga ccgtcgtgca
     1681 gaaggatgcc agcttcgcgg gatcgaagcg acgtccggtg tcttccatta gtagtctctc
     1741 ctccaggcaa gtctcgtatc gacagacggg ccgctcgcca ggatgtggta gatcccagag
     1801 cagccgctac cagatggtcg ccaacacctg gcttccactg gcctctcggg tcaacctggt
     1861 ccggtcggct ctgtggtacg ccctgaagaa ccccatcacc tcacccgacg gaaggaaggc
     1921 cctcgtctcc accaccagca gaggagagcc cgatggaacc cgcaggatgt tggaccggaa
     1981 gctgtccgcc tgaatgcact cgatcgtctc ctctcccttc tcgggtgtca gacctgcctt
     2041 gcagatgagg agctgagaga agctctcgga ctcgagctcc ggccgagtga actcaggaaa
     2101 caggcggggc agaaggaaca gggtaacgta ggccaatggc tcatcctcca ccaggcgaac
     2161 ccggcgaatc ctgtagagct cctcaccctt cggcacgccc agcttgccag cctgccactc
     2221 gtcggccgag atgcggtcaa agctgtagag catgaccttg gcggccttcc cctccagcga
     2281 ggaggtgctg ctatccagcc ttgacgctat cttcggcgga agcacgtacg tgccccgccc
     2341 tctctgcgta gccaggattc cttcccgaac gagggacgag acggcctgcc gcacggtaat
     2401 acggcttacg ccgaattgag cgcacagctc cggctcagtt ggaatcctgt cacccggcgc
     2461 cagttccttc agtgccttgt cgcgcaactc gatcttaacc tgtcgccaca gagtgagcgg
     2521 cgaatctttg gcaagcatag ccagatgcct ccagccagta tcaggacttc tcgaccatct
     2581 tcagggccgc tcgacagatg gctagggcca tcttgtccgc ggtctcccag acatccggtt
     2641 tgaatactcc atccgcactc tcgtccgtgc cgccacacat gcagtgatgg acacgcgctg
     2701 cgtccacgtg gagtctcttc gggcgaggaa gcccttgcca cagtttccag gcaacggcga
     2761 tatcggtgga gtaggccggt gcctcgccct cacccttcac caccttctcg tgtatcaacc
     2821 tgtccaattc agatcctgcc tgaagttgca gggtcatcgg tatctcctca tcgtcaaaat
     2881 cactctggga aaggctaagc gatggcctgg agtcctgctc gtacgatctc ctcgtcctcg
     2941 accgaagttc cgccgctgac tcccgccgct ccgacgacct tgccgtcgat cacgatcggc
     3001 agagccccct gtcggatcac gccacggcca cccgatgcgg tcacgacgct ctcgtagaag
     3061 tacggcgcag cctgtgcctt cttctcgata tcgccgctgc ttatcctcca cgcggaggac
     3121 acccacgcct tgcccatggc gatgttcggc gagaagaagg gcgcgccatc catgcgcgcg
     3181 acgtagacga gattgccgga ttcatctagt acggcgaagc ttaccttgag gcccatttcc
     3241 ctagcgcgct cgagaccctt ctgggcgatc ttttccgcgc tagccaaatc cagagccatt
     3301 ttcatttctc cctatagtgc tgttctcgtt ttccgctcaa aatgttgtta ccgccgggcg
     3361 ggttgcccca acccgcccgc atggctctca ggacgccacg ctgctcttca ccgtcacgtc
     3421 cgcaagcccc tccaggacgg tcacgaccgc ggccgtaacc aggcttccca gccccttcgc
     3481 cctggccgcc tggaagatct ccctcacccc cgaggtgaca gggaggggca cctggagttg
     3541 ctccgccagc ctgaccgcgt tggtgaggtc cttgtgctgc gtattgatgg atgaacgcgc
     3601 ctcgaagtcc ctggccagga tcctcgggcc gcgggattgc attgcgaagg tccgcgctcc
     3661 tcccaccgag gtgatgtcga ttaccagagc cggatcgatt cctgccttgg tggccaggac
     3721 cagtgcctcg cacgctgcct caatggctac gttcaagacc aggttattga ttatcttgag
     3781 ggtagctccc gaaccagttg gcccggcata gactatcttg gtacccaacc gctcaaagac
     3841 cggcagacaa cgctcaagag cctctttcgg tcccccgatc atgatgccga ggttgccctc
     3901 cgccgccacg ctgccgttcc cggtaacggg cgcatcgagc attgagacgc ccagagcctc
     3961 ggccttcctg gagatctcgc gggaggtttc tggatctatg ctccccatat caacgaggac
     4021 catcccagga tgagcgccct ccagaactcc atcaggaccg caagcgacct cctcggaggc
     4081 atgggagttg gcaaccatgg tgatcacgat gtcggacgct gcagcgacct ctttagggga
     4141 agcggcggcc ctggcaccta actcaaccaa cggccgaacc ttgtctgggc tccggttcca
     4201 gaccgtcacc tgatacccac cggagaggag cctccgcgcc atggagt
//
LOCUS       DATALS010000217         4885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_379706, whole genome shotgun sequence.
ACCESSION   DATALS010000217 DATALS010000000
VERSION     DATALS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379706"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            530..1102
                     /locus_tag="VHS06_04795"
     CDS             530..1102
                     /locus_tag="VHS06_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX2987470.1"
                     /translation="MTQQDALKGMRVAIIVADDFEQAEMTEPRKALDQAGAHTVLVSP
                     VRGEVQGVHHDVKADRFKVDLPIDQARPEDFDALLLPGGALNADFIRMNEKAREFVRS
                     FDRSGKPMAVICHGAWLLVSSGVARGRTLTSYHTIQDDLRNAGANWVDQEVVHDGNLV
                     TSRQPSDIPAFNREMIQLFSQSLKNMRKAA"
     gene            1154..3181
                     /locus_tag="VHS06_04800"
     CDS             1154..3181
                     /locus_tag="VHS06_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005040652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2987471.1"
                     /translation="MHQHEHQAHEGAPSQAIPSGSHEGHVVEEFERRFWVCLALTIPI
                     LVYADLPQEVFHYSAPTFPGSQLLSPLLATVIYFYGGIVFLRGAGAELASRTPGMMTL
                     VALAITVAYGYSLAVQFVLGGMPLYWELATLVDIMLLGHWVEMRAVGRARGALAELAK
                     LMPDEAERVVQGGTELVPVQEIRVGDLVLARPGGRVPADGIVETGESHVNESMITGES
                     RPVPKAPGDRVIAGSVNEEGSLRVRVNRVGEDTALAGIMRLVERAQHSRSAAQALADR
                     AAGWITYAAVAVGLITLVGWLLLAAGVSFALERMVTVLVIACPHALGLAIPLVISIST
                     GLAARNGLLVRERMALEQARDLDVIVFDKTGTLTRGEQGVAGVAAIEGLTVEDALMLT
                     AAVEGDSEHAIARAIRGEAERRGLVLPAAEGFQALVGRGVRAVVRGRVLRVGGPRLLE
                     ATGVEPPTELRERAEDWGRQGKSVVFLLEDDRVMAALALADLIRPESKEAVSQLSSLG
                     IEVAMMTGDSEDVAASVARELGIHRYFARLLPEDKAGRVQELRRQGSRVGMVGDGIND
                     APALLMANVGIAVGAGTNVAIESAGIILVRDDPRDVVKVIRLSHASYRKMVENLAWAT
                     GYNVIAIPLAAGVLAPFGIFLAPAVGALLMSASTVIVALNAQLLRWTRLSQ"
     gene            complement(3285..4046)
                     /locus_tag="VHS06_04805"
     CDS             complement(3285..4046)
                     /locus_tag="VHS06_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2987472.1"
                     /translation="MPKILVVDDEPVLLNTLRPIIERAGFEVLAATDGDAALKMARQE
                     MPDLIILDVMLPRITGFEVCRILRQESTIPIIMLTAMGDELDKVVGLEAGADDYLTKP
                     FGAWELLARMKAVLRRSGMAQHGDKEAQAPDKASQTNILKEGDLQIDLISRRALLAGK
                     HLDLKPRVFDLLAFLVHNRGRVCSQQELLNRVWGYEDDSDTRTVAVHIRHLRRKLEAD
                     PSKPRLIKTVRGVGYCYSPHDPESIAIGAATVPEN"
     gene            4421..>4885
                     /locus_tag="VHS06_04810"
     CDS             4421..>4885
                     /locus_tag="VHS06_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEX2987473.1"
                     /translation="MRVTLQTKMLAGFAAVLLLTVISGGIGILGMGRVSGMMTSMYDD
                     NLLPIQYLGAAGSSMERSRVYLMEALGTDDKTATADAEKKIADNDAAMMKSVEYYRKS
                     KLGAEETGLLAKFDSSWKAYQEDRDSLLRLHREGKDQASISLALVSAREKLDT"
BASE COUNT          919 a         1358 c         1647 g          961 t
ORIGIN      
        1 acggaaacgc tcttgttggc accgatttct gttggatgat acttccatta ttgggtactc
       61 tggaaggcat gtcaatatta gtatccgaat actcgcagtt tgaaagctgc atcctaaagc
      121 cagtgttcat gcctgtttcg ctgttcattg tctcattgcg tattccgctg gcccaaggcc
      181 gaagtgagca ccaaccgcga agagcaaacg aacaggatgg acaggatgta caggataaga
      241 tcgggatcct cggtccatat cctgtctatc ctgtttaccc tgttaggatc ggtcccccac
      301 gcgtacccgc atcacggcct tgcgggcgag tcgcccgcgc tcccaggatt ttgcacgctg
      361 gcacaatcct tctcagccct catcccgtga acctgccctc tggcttacag ctcccgcaga
      421 gggagaggga gtacactctc tccgcgtctc cgggcaccgg tggcatagcg attgccgtct
      481 tccttggcgc ccaccaaggg acatgcaaaa agacgtcggg aggttctttg tgacccagca
      541 agacgctctg aaggggatgc gggtggccat catcgtggcc gacgatttcg agcaggcgga
      601 gatgaccgag cctcgcaagg cactcgatca ggcaggtgcc cacaccgtcc tggtctcacc
      661 ggtcagagga gaggtgcagg gggtccatca cgatgtaaag gccgatcggt tcaaggtgga
      721 tctgccgatc gaccaggcga ggccggagga tttcgacgcc cttttgctcc cgggtggcgc
      781 tctcaacgcc gacttcatcc ggatgaacga gaaggctcgg gagttcgttc gcagcttcga
      841 tcgatcgggg aagccgatgg cggtgatttg ccacggggcc tggctgctgg tgtcgtcagg
      901 ggtcgcacgg gggcgaaccc tcaccagcta tcacacgatc caggacgacc tgcggaatgc
      961 cggcgccaac tgggtcgacc aggaagtcgt gcatgacggc aacctggtca caagccgcca
     1021 gccatcggat atccccgcct tcaacaggga aatgatccag ctcttctcgc agagcttgaa
     1081 gaacatgcgg aaagccgctt agccgggccg gatggcaggg agggtccatc tggccctccc
     1141 tggacctagc tccatgcatc agcacgaaca ccaggcacac gaaggtgctc catcgcaggc
     1201 gatacccagt ggttctcacg aaggccacgt ggtggaggag ttcgagcgcc gcttctgggt
     1261 gtgcctggcg ctcacgattc ccattctggt gtatgcggac ctgccacagg aggtcttcca
     1321 ctactctgcc ccaaccttcc cgggcagcca actgctgtcg ccgctgctgg cgaccgtcat
     1381 ctatttctac ggaggcatcg tattcctgcg gggtgctggt gccgagctag cctcgcgtac
     1441 cccagggatg atgacgctgg tggcgctggc cataacggtg gcctacggat acagcctggc
     1501 agtgcagttc gtcctcgggg ggatgcccct ctactgggag ttggcgacgc tggtcgacat
     1561 aatgcttctc ggccactggg tagagatgag ggcggtcggg agggctagag gggcattggc
     1621 tgaactggcg aagttgatgc cggatgaggc ggagagggtg gtgcaggggg gcaccgaatt
     1681 ggtaccggtc caggagattc gggtgggaga cctggtgctg gcccgaccgg gggggagggt
     1741 tcccgccgac ggcatcgtgg agacgggcga gtcccacgtc aacgagtcga tgatcaccgg
     1801 ggagtcccgt ccggtgccca aggcccctgg cgacagggtg atcgcgggga gcgtcaacga
     1861 ggagggctct ctgcgggttc gggtgaaccg tgtcggcgag gacacggcgc tcgcggggat
     1921 catgcgcctg gtggagaggg cgcagcactc tcgctcggcc gctcaggctc tggccgaccg
     1981 tgcagccggc tggatcacct atgccgccgt cgccgtcggg cttatcacgc tggttggctg
     2041 gttgctgctt gcggctgggg tctcgtttgc cctggagagg atggtcacag tccttgtgat
     2101 cgcctgcccg cacgcgttgg ggctggccat accgctggta atctcgatat ccacgggact
     2161 cgcggcccgg aatggcctgt tggtaaggga gcggatggcg ttggagcagg cgcgtgacct
     2221 ggacgtgatc gtctttgaca agactgggac cttgacgcgt ggggaacagg gggtggccgg
     2281 cgtcgccgcc atcgaggggc tgacggttga agacgccctg atgttgacgg ctgcggtcga
     2341 gggcgactcg gagcacgcta tcgctcgagc gattcgaggc gaggccgagc ggcgaggcct
     2401 ggtgctgccg gcggcagagg gcttccaggc gctggttggc aggggcgttc gggcagtggt
     2461 gagggggcgg gtgttgcggg ttgggggccc gcggctgctg gaggcgacgg gcgtggagcc
     2521 gccgacggag ctgcgagagc gagctgagga ctgggggcgc cagggaaaga gcgtcgtatt
     2581 ccttctcgag gacgataggg tgatggccgc cctggcgttg gccgatctca ttcgcccgga
     2641 gtccaaggag gcggtctccc aattgagctc gctagggatc gaggtggcaa tgatgaccgg
     2701 tgacagcgag gacgtggctg cctcggtcgc gcgggaattg ggcatccatc gctactttgc
     2761 ccgccttctg cccgaggaca aggccgggcg ggtgcaagag ctgcgtcgcc aagggagtcg
     2821 cgtaggaatg gttggcgatg ggatcaacga cgcgcctgcg ctgctgatgg cgaacgtggg
     2881 gatcgccgtg ggcgccggaa cgaatgtggc catcgagagc gcggggatca tcctggtaag
     2941 ggacgaccca agggacgtgg tgaaggtgat aaggctcagc catgccagct accgcaagat
     3001 ggtggagaac ctggcctggg caaccggcta caatgtgata gcgatccccc tggccgcggg
     3061 agtgctggct cccttcggga tcttcctggc accggcggtt ggtgcactgc tgatgtccgc
     3121 cagcacggtg attgtcgcgc tgaacgcgca actgcttcgg tggacgaggc tctcgcaatg
     3181 atggactctg ggcatgtgcg gcgtaaagcc gggtccaggg tgcgcgatgc gagcgagcgc
     3241 agcgtcacgc cgggctccta tgatcaggag tacggtatca gggactagtt ctcaggaact
     3301 gtggcggcgc cgatggcgat cgactctgga tcgtgtggcg agtagcagta cccgaccccc
     3361 cttactgtct tgattaggcg gggcttagat gggtctgctt ccagtttccg tcgtaggtgg
     3421 cgaatatgga cggccacggt tcgagtgtcc gagtcgtcct cgtagcccca cactcgattc
     3481 aacaactctt gctgggaaca gacgcgtccg cggttgtgca ccaagaaggc cagcagatcg
     3541 aacactctgg gcttcagatc caggtgcttt ccagccagta gcgcacgacg gctgatcagg
     3601 tcgatctgaa ggtcgccctc tttgaggatg tttgtctgcg atgccttgtc tggggcctgc
     3661 gcctctttgt ctccgtgttg ggccatgcct gaacgccgaa gcacagcctt cattcgtgcc
     3721 agcagttccc acgcgccgaa gggtttcgtg aggtagtcgt cggcccctgc ttcaagtccg
     3781 accaccttgt ccagttcgtc gcccatagcc gttaacatga tgatggggat tgtgctctct
     3841 tgcctgagga tgcggcagac ctcgaatccg gtaatcctcg gcaacatgac gtccaggatg
     3901 atcaggtcgg gcatttcctg cctcgccatc ttgagagccg catcgccgtc tgtggcggcc
     3961 aggacctcga acccggccct ctcgataatg gggcgaaggg tgttcagcag aactggctca
     4021 tcgtcaacca cgagtatctt tggcacctta gctggatccc ctcccctggc gataccgacg
     4081 gggcgatggc cagccctact gtttcaggca gtcacggcac agtgcgcccc cagtcagata
     4141 tctgtgtcaa agaatgtgca acctgcaaac ggggtctgga tggggatgca taccgagacc
     4201 ttgcagcaac cttaacaagg attataccaa ctgggcccga gactgtgcga gcgcaattgt
     4261 tgagttagca gatgttagag caaggttatc gaccggtaag cgtccagggt tcttcaagac
     4321 cgtagaatac ctgtagaaga acgatcgccg taccggcgac cagaatcacg cccgagtcgt
     4381 cgagttgggt gtcaactcgg ttcgggaagg agaacccctg atgcgtgtca cacttcagac
     4441 aaagatgttg gccggcttcg cagcggtact gctgcttacg gtcataagcg gagggatcgg
     4501 cattctcggc atggggaggg tgagcggtat gatgacctcc atgtacgacg acaatctgct
     4561 gccgatccag tacctgggtg ctgccggatc ctccatggag aggtcgcggg tctacctcat
     4621 ggaggctctc ggtaccgatg acaagacagc gacggcggat gcagaaaaga agatcgcgga
     4681 caacgacgca gcgatgatga agagcgtaga gtattacagg aagtcgaaac ttggcgcaga
     4741 ggaaacgggt ctactggcca agttcgactc ttcctggaag gcctaccagg aagataggga
     4801 tagcctgctg cggcttcatc gagagggcaa ggaccaggca agtatcagtt tggctctggt
     4861 gtccgctcga gagaagttgg acacg
//
LOCUS       DATALS010000218         3723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_156769, whole genome shotgun sequence.
ACCESSION   DATALS010000218 DATALS010000000
VERSION     DATALS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156769"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..551)
                     /locus_tag="VHS06_04815"
     CDS             complement(114..551)
                     /locus_tag="VHS06_04815"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX2987474.1"
                     /translation="MLGIDARRTIVGLSLLGAADSIYMLSYHLGLIGSLVCPFFGEGC
                     NRVGRSSHARHFGVPNAAVGAAGYGAMAVLAAMSGGQKGRRWSAPLLAATSLSAFGAS
                     VFLTWEQPFRVGAWCFWCLSSAAINLAILPLALRGAARKGDRL"
     gene            complement(668..1378)
                     /locus_tag="VHS06_04820"
     CDS             complement(668..1378)
                     /locus_tag="VHS06_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEX2987475.1"
                     /translation="MTTEVEAAGAAGPKPLVTVEMVRLSNEIRCYQEAADQVAIQKGY
                     TEHGLRHVSLVSTVTHGILTQLQAPPRRAELGAIAGYIHDVGNLVGRQEHAASSGIIA
                     MHLLHDMGMPPEEIAVVVGAISNHDEGEGEPVSDVSAALILADKSDVHRSRVRNPKPE
                     TFDNHDRVNYAARRSYVRVDAVRRVIAYDLTIETECLPGGVLDYFEIFMPRMLLARKA
                     AKFLGCQFELIINNTRVF"
     gene            1609..1899
                     /locus_tag="VHS06_04825"
     CDS             1609..1899
                     /locus_tag="VHS06_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987476.1"
                     /translation="MTVRRLGALVAEAVASGVGVLVEAITGAALVGSTATVGMVLGVG
                     LDTKGRSREPLLAVGMGVTAAATGTVVGSCVEVGLTDALRWFIEMSPTKPRQ"
     gene            complement(2172..>3723)
                     /gene="serA"
                     /locus_tag="VHS06_04830"
     CDS             complement(2172..>3723)
                     /gene="serA"
                     /locus_tag="VHS06_04830"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX2987477.1"
                     /translation="AEDGIEYLKQHAEVDVKTGMAPRELIEIIPNYDALVVRSETKVT
                     SEVINAGTRLQVIGRAGVGIDNIDVTAATRRGIVVVNAPTGNNIAAAEHTIGMMLAVA
                     RHIPQAYLSLRSGAWKRSAFMGVELRDKVLGVVGLGKIGTEVAKRAQGLAMKVIAYDP
                     YLSAEHAKKLGIDAVTLDDLVQKSDFITIHVPLTAQTRNLIGAERLGKMKPTARIINC
                     ARGGIVDEAALLQALEAGTIAGAALDVFSKEPLPADSPLLKSDRVVVTPHLAGSTVEA
                     QVGVALDVAEQIVDVLSGKSARYAVNAPVILPETMELLSPYLELAGKLGSLATQLSDG
                     QLRSIEVTYAGELAELDTAPVKAMLIKGLLQPILAEEINLVNAPVIARSRGLNIVERK
                     TSQVNAYSNLITIQLDTDRSSHVVAGTVFFREPHLVRVDKYWVDVVLAGGHMLFSYHH
                     DRPGMIGHVGTVLGDANVNISFMAVGRLSAKGEALMVIGVDDVVGPELVPKLLAVPDI
                     FAIRTVNI"
BASE COUNT          744 a         1204 c         1076 g          699 t
ORIGIN      
        1 aagccgcaaa accggtcaat gagcttattg tcgataaccc tgtggatatt tcgacggtaa
       61 tgtggataaa aaccaagcgg acctcagtct ggcagagacg ctagatgccc gacctatagc
      121 cggtcacctt tcctcgccgc gcctctcagc gccaacggca ggattgccag attgatcgcc
      181 gcggaactca ggcaccaaaa gcaccatgcc cccacgcgga acggctgttc ccaggtcaga
      241 aacacactgg cgccgaaagc agaaagagag gtggcagcca gcagaggcgc actccagcgg
      301 cgccctttct gtcccccgga catcgccgcc agaaccgcca tggcaccata accggccgcg
      361 cctaccgctg cattgggaac cccaaagtgt cgagcatggg acgaccgccc caccctgttg
      421 cacccctctc cgaagaaagg gcaaactagg gagcctatca ggcccaggtg gtaggagagc
      481 atgtagatgg agtcggccgc acccagaagg gatagaccga cgatggttcg gcgagcatca
      541 atgcccagca actcaacctc ccacatttgg catcgtagga ggctggtagc agaagacgtg
      601 cctccgtcgc ggagggaggt gcgtgttcta agtgggaggg gcggaccttc gtgtccgccc
      661 ctcccactta gaacacgcgg gtgttattga tgatcagctc gaactggcag ccgaggaatt
      721 tcgcggcctt cctcgccaaa agcattcgcg gcatgaagat ctcgaagtag tccagcacgc
      781 cgccgggcag acactccgtc tcgatggtca agtcgtaggc gatgactcgc cttaccgcat
      841 ccacccgcac atagcttctt cgtgccgcat agttgaccct atcatgattg tcgaaggtct
      901 ccggtttggg gtttctaacc cggctgcgat gcacgtcaga cttgtccgcc aggatcaagg
      961 cagcactcac gtcactcacc ggctcgccct cgccctcgtc gtggttggag atggccccca
     1021 ctacgaccgc aatctcctcc ggaggcatgc ccatatcgtg cagcagatgc atagcgataa
     1081 tcccgctgga agcggcgtgc tcctgcctgc ccaccaggtt gccgacgtcg tggatgtatc
     1141 cggcgatggc ccccaactct gcccgtctgg gaggtgcctg cagttgcgtc agaatgccgt
     1201 gggtcaccgt ggaaaccagg ctgacgtggc gaagaccgtg ttcggtgtac cccttctgga
     1261 tggccacttg atccgccgcc tcctgatagc agcggatctc gttcgacaac ctcaccatct
     1321 ccacggtgac caacggcttc ggtcctgccg ctcctgcggc ttcgacttca gtggtcatga
     1381 tcggatccct cctgactact ctatcgagag acgggcgcgg aagtcggcac ggccgggaca
     1441 cgagtagctg tggcggccgg tgccgccgcg cgtggggtcg ccgtcgccgg caccggcgtg
     1501 gcaaggatca gtccctgccc ggtggttggc gtcgcgggga taggggtcga taccacctga
     1561 ctcggaacag ctgtcaccaa accaactggg gtggaactgg atactggggt gacggtacgc
     1621 cgactcggag ccctggttgc cgaggcagta gcctccggcg tcggcgtctt ggtggaagct
     1681 atcacgggag ccgctctggt cggctctact gccaccgtcg gtatggtcct cggggtggga
     1741 ttggacacga agggacgctc tagggaaccg ctgctggccg tgggcatggg agtcacggcc
     1801 gccgcgaccg gcaccgtcgt gggaagctgc gttgaggtgg gcttgaccga cgcattgcgc
     1861 tggttcatcg aaatgagtcc gacgaagccg agacagtaga gccaggagga aagaagaaac
     1921 aacgcggtcg cgctcgccac cgcgatcttc ttcaaatcgc tccactgctc aaaccgatcc
     1981 cacagaggaa gcagcctatc atcaaaccag tcgcccaaag cagccaatct atccacgggt
     2041 cgttacctca caaagctggc agccgtcagc tatcggttgc agcatctacc ggccggaacc
     2101 ggcagacact tattcactca cgaagcgcag aaagaagagg ggagagcaga actcttccgc
     2161 gtgatcgagc cctaaatgtt caccgtgcgg atggcaaaga tgtcgggcac cgccaacagc
     2221 ttcggcacca actccggccc aaccacgtcg tccacgccga tcaccatcag tgcctcaccc
     2281 ttcgcagaga gccgccccac cgccatgaat gagatgttta cgttggcgtc tcccagcacg
     2341 gtgccgacat ggccgatcat gcccggccgg tcatggtgat agctgaaaag catgtgccca
     2401 ccggcgagca cgacgtctac ccagtacttg tccacccgga cgaggtgagg ctccctgaag
     2461 aataccgttc ccgcaaccac gtgggaggaa cggtcggtat ccagttggat ggtgatcaaa
     2521 ttgctgtagg cgttaacctg gctggtcttc ctctccacga tgttgagacc cctgctccgc
     2581 gcaatgaccg gagcattgac caggttgatc tcctcggcaa ggatcggctg caacagcccc
     2641 ttgatcaaca ttgccttcac gggggcggta tccaactcag caagctcgcc ggcgtaggtg
     2701 acctctatac tgcgcagctg cccatctgag agctgagtgg ccagactccc cagcttgcct
     2761 gccagctcca ggtaggggct cagcagttcc atcgtctccg ggagtatcac cggtgcgttg
     2821 acggcatagc gagccgattt tccgctgaga acatccacga tctgctccgc cacgtccagc
     2881 gccactccca cctgagcctc gactgtggag ccggccagat gcggcgtcac caccacccga
     2941 tcgctcttca gcagggggct gtccgccggc aacggctcct tagaaaagac gtccagcgcc
     3001 gctcctgcaa tcgtccctgc ctccagcgct tgcagcagcg ccgcttcgtc gacgatgcca
     3061 cctcgagcgc agttgatgat ccgggcagtg ggcttcatct ttccaagccg ctccgccccg
     3121 atcagattgc gagtctgggc cgtcagcggc acatgaatag tgatgaagtc ggacttctgc
     3181 acaagatcgt ccagcgtcac ggcgtcgatc cccagcttct ttgcgtgctc tgcagacaga
     3241 taggggtcgt aagcgatcac cttcatcgcc agcccctggg cacgcttcgc gacctcagtc
     3301 cctatcttgc ccaggccgac gacacccagc actttgtccc tgagttccac tcccatgaag
     3361 gcagaacgct tccaggcgcc cgagcgaagg gaaaggtacg cctgcgggat atggcgcgcc
     3421 accgccagca tcattccaat ggtgtgctcc gcagcggcga tgttgttgcc ggtgggcgcg
     3481 ttcacgacca ctatcccacg acgggtagcg gctgttacgt cgatgttgtc gatccccaca
     3541 ccggcccgac cgatcacctg gagcctggtt ccggcattga taacctcgga ggtaaccttg
     3601 gtctcgctgc gcactaccag tgcatcgtag ttgggaatga tctctatcaa ctcccgaggc
     3661 gccatgccgg tcttcacgtc gacctcggca tgctgcttca ggtattctat cccgtcctct
     3721 gcc
//
LOCUS       DATALS010000219         4648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_247303, whole genome shotgun sequence.
ACCESSION   DATALS010000219 DATALS010000000
VERSION     DATALS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247303"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..2986
                     /locus_tag="VHS06_04835"
     CDS             95..2986
                     /locus_tag="VHS06_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2987478.1"
                     /translation="MGSSRSRNETDGGSWAFQAEMLLEVAALLNSTLERDQVLGTLAQ
                     QFLRMVAADWVVVVELSDSTDEEFSLVAGAYKGGRALELGQSARLALAANPFLSRALV
                     TRSPVWVQDVAEAQSSVGLLGVLGAESVLSVPLIFRDAALGVLAAGWEKPVGGPSSRE
                     MRLIQGLANQAATAMENARLYAAAERRAQQVTALFEIGRDISANLALSEILTSIVGKA
                     QELLESDTSFLALLSPDGQELEMVASVGLRSEAMRTLRLKREQGLAGVVVSRGEPIIV
                     DDYPREVIFKDPPMDAVHEEGLRSEIGVPLINGKQLLGVLYIGNREAKRFNPEHAQLL
                     MAFAKQATIAIQNARLYEVVVAQRERAEAGRRDLQVVIDSMPEGVVIAEGADGRISTI
                     NRAGKLLMGIADVVQLPREAAILSPERGSGLPSFFTPNGSPYPWEDMPLSRSIRSGEV
                     ILGVEVVIKRPDGTHVSVLSNSAPFRDLDDKVAGAVFVFQDISKSKEAEQLKDEFISL
                     VSHELRTPLTSIKGAARTILRHYNTLDPETRGELIRDIDEESDRLYRLVENLLDFSRA
                     EAGVLRLATEPVHLPKLLERVVGEASSRSTGYHFVLHLPADLPPAEADPIRVEQVLRN
                     LLDNAIKYSPNGGEIRVDAAVENGMLRVSIKDQGVGVPQEDRERVFGRFERGGGSDSS
                     RPHGVGLGLAICRRLVEAHGGTIWVDSGYGGGLDILVYPSACRGGVGMMKSCSILVVD
                     DEPKILRFVRAELEAAGFRVSTATDGHSALDLLAKDGADLVVMDVIMPVMDGFEVLKR
                     IRSSSSVPVILLTARGSDADKVRGLDLGADDYLTKPFSPDELSARVRAVLRRTAASSE
                     NPLGRPFSVGELKVDFDRRKVVVRGQEVSMSRTEWQLLQQLALNAGKVMLHEELLVRT
                     WGPEYRDDVEYLRVWVSRLRRKIEDDPARPVYLKTVPGVGYVMADGNPL"
     gene            complement(3157..3531)
                     /locus_tag="VHS06_04840"
     CDS             complement(3157..3531)
                     /locus_tag="VHS06_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987479.1"
                     /translation="MQDSSPKVLIAEEDDRLRRIVRLNLEQGGFSTTEAASLQECELA
                     IQRDHVALVIVSSQLPDFDGPHFTQWLRSHFPLDPVPVVLVSFEPEDRLYTVPLKQAA
                     FERKPFDPEELVDQVTRLLHTA"
     gene            3839..>4648
                     /locus_tag="VHS06_04845"
     CDS             3839..>4648
                     /locus_tag="VHS06_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987480.1"
                     /translation="MKKRSFVILLAIAAVPLLLGVSGGTPSSPGYRFARQSAPYTETV
                     AAWMAQNSVEKVAETLTVSPASVEQVRSVFRLMGRRLGTLPSTTSSSSSGIVQPVSVS
                     SEPLPSDRDLAEQQPAVEERIQRQVATVLHRRGVGFKVGDGSFLFPPVLFRFRDLPDL
                     LVVSPRSRIERLGTVLLTPNMPLTDAVKLEGEVSSGGYSALVTHIGGLGVYPSMVPDN
                     SDLRWTTRTVAHEWAHQFLALRPLGWRYAFGAERDPRMVMLNETAAEVIGRE"
BASE COUNT          865 a         1303 c         1540 g          940 t
ORIGIN      
        1 gtagcagagt ggcgtggtgg cggcgaacca ccggctgaga tatcatgaca gtgataaaat
       61 tgtcattctt tgccgcaatt taggggtgct gccggtgggt tcttcacgat ccagaaacga
      121 gaccgacggg ggatcctggg cctttcaggc cgagatgctg ctggaggtgg ctgcccttct
      181 caactccacc ctggagcgcg atcaggtgct cggcaccctc gcccagcagt tccttcggat
      241 ggtcgcggca gattgggttg tggtggtcga actttccgat agcaccgatg aggagttctc
      301 cctggtggcg ggggcgtaca agggtggccg cgcgctggag ttgggacaga gcgccagatt
      361 ggcattggcc gcaaacccct tcctgtctcg ggcattggtg acacgctctc cggtatgggt
      421 gcaagacgtg gccgaggcgc agtccagtgt cggcctgctg ggtgttcttg gcgcggagtc
      481 ggtactctcc gtcccgctca tcttcaggga tgccgctctc ggcgtgctgg cggccgggtg
      541 ggagaagccg gttgggggtc catccagccg ggagatgcgg ctcatccagg ggcttgccaa
      601 ccaggcggcc acagcaatgg agaacgcccg cctgtatgcg gcggccgagc ggcgggcgca
      661 gcaggtgact gccctcttcg agattggcag ggatatctcg gccaatctcg cgctgtcgga
      721 gatcctcact tccatcgtgg gcaaggcaca agagctcttg gagagtgata cgagcttcct
      781 cgcgctgctt tcccccgacg gccaggagtt ggagatggtt gcctcggtgg gccttcgcag
      841 cgaggcgatg cgcaccctga ggttgaagcg tgagcagggg ctcgccggag tggtggtgag
      901 tcgaggcgag ccgatcatcg tggacgatta cccgcgggag gtgatcttca aggacccgcc
      961 catggatgcg gtgcacgagg agggattgag gtctgagatt ggcgtgcccc taatcaacgg
     1021 gaagcagttg ctgggcgtgc tgtatatcgg taaccgcgaa gcgaaacggt tcaacccgga
     1081 gcatgcccag ttgttgatgg ccttcgccaa gcaggccact atcgccattc agaacgcacg
     1141 actgtacgag gtggtggttg cccagcgaga aagggcggag gcgggacggc gcgaccttca
     1201 ggtggtgatc gacagtatgc cggagggtgt ggtcattgcc gagggggcag atgggcggat
     1261 ttcgaccatc aaccgtgccg gcaagttgct gatgggcatt gcggatgtag tccagcttcc
     1321 cagggaggcg gccatcctgt cacctgagcg tggtagcggt cttcccagct tcttcacccc
     1381 caacggctcc ccgtatccgt gggaggacat gcccctgagc cggtcgatca ggtccggtga
     1441 ggtgatactc ggcgttgagg tggtgatcaa acggccggac ggcactcacg tgtcggtgct
     1501 cagcaacagt gcccctttca gggatctgga tgacaaggtc gccggtgccg tgttcgtatt
     1561 ccaggacatc agcaagtcga aggaggccga gcaactgaag gacgagttca tctccctggt
     1621 ctcccacgag ctgcggacgc cgctgacctc aatcaagggc gcggcgcgca ccatactccg
     1681 ccactacaat acccttgatc cggagacgcg cggagagctg atcagggata tcgacgagga
     1741 gtcggatcgg ctctatcggc tggtggaaaa cctgctggac ttctctcgcg ccgaggcagg
     1801 ggtgctgcga ttggccaccg agcccgtgca ccttccaaag ctgttggaga gggtcgtggg
     1861 tgaagcgagc agccgttcca ctggatacca tttcgtcttg cacctccccg cggaccttcc
     1921 ccctgcggag gcagatccca ttagggtgga gcaggtcctg cgcaaccttc tcgacaacgc
     1981 catcaagtac tctcccaacg gaggagagat tcgggtggac gcggccgttg agaatgggat
     2041 gctgagggta tcgatcaaag accagggcgt cggagtgccg caggaggacc gagaaagggt
     2101 gttcggccgg tttgagcgtg gcggtggcag cgattcgtca cgccctcatg gggttgggct
     2161 gggactggcg atctgtcgca gattggtgga agcgcacggc gggacaattt gggtagactc
     2221 aggatatggg ggggggctcg acattctcgt ttacccttcc gcttgtcgag gaggcgtggg
     2281 gatgatgaag agttgctcga tactggtggt ggatgacgag ccgaagatcc tccgtttcgt
     2341 tcgggcagag ctcgaggcgg ccggctttcg cgtctccacg gccacggatg gccattctgc
     2401 cctggacctt ctcgcaaaag atggtgccga cctggtggtg atggacgtga tcatgccggt
     2461 gatggacggc ttcgaggtgc taaagcggat tcgcagctct tcctcggtcc ccgtgatcct
     2521 actgacagca cgagggagtg atgccgacaa ggtccgaggg ttggatctgg gtgcggatga
     2581 ctacttgacc aagcccttca gccctgacga gctgtcggca cgagtgaggg cggtgctcag
     2641 acgaaccgct gccagcagcg aaaaccccct ggggcgtccc ttctctgtcg gtgagctcaa
     2701 ggtggacttc gatcgtcgca aggtggtggt ccgaggtcag gaggtgtcca tgtcccgtac
     2761 agaatggcag cttctacagc agcttgcgct gaatgcaggc aaggttatgc tgcacgagga
     2821 gttgctggtg cggacctggg gcccggagta ccgggacgac gttgagtacc tgcgggtgtg
     2881 ggttagcaga cttcgcagaa agatcgagga cgatcctgca cgcccggtct acctgaagac
     2941 ggtaccgggt gtgggctatg tgatggccga cggcaacccg ctgtagagac aatatcgggt
     3001 cgaaatcagg gagccggcac gccattgtgc aggctcccat catttgtgtg aagacccttg
     3061 aggcgagggg cgaaggcacc gtcgcctcgc cacttacctc tcggtaaccg caccccattg
     3121 tgcaggcggc tgggtttagc acattcggta ccgcagttac gcggtgtgga gaagcctggt
     3181 aacctggtca accaactctt cggggtcgaa cggttttcgc tcgaatgcgg cctgtttcaa
     3241 aggcaccgta tagagcctgt cctcaggctc gaaggaaacc agcaccaccg gcacgggatc
     3301 gagcggaaag tgagagcgca gccactgggt gaaatgcggt ccatcgaagt cgggaagttg
     3361 ggaactgacg atgaccagcg cgacatgatc cctctgaatt gccaactcgc actcttggag
     3421 ggacgctgct tcagtagtcg agaagccgcc ttgctccaga ttgagccgca cgatgcgtct
     3481 caaccgatcg tcctcctcgg ctatcaacac ctttggactg ctgtcttgca acgttggatc
     3541 tacctgcttc attgcccttg cccagcgtcc tcgccgacgc cgttccagtc ggtggagaaa
     3601 cggtaacact accaccctat caaaggcata aaacggcacc gtgaaaggtt gcaaaagcat
     3661 tacagcggac ggcagcacgc ccggttccct gccctccggg tgctacgagg atagccggcc
     3721 ggcgtgctgg agccgcacct accgacaaat tgctgaggtg tagttcctgc tgtagaatcg
     3781 tggccaggga aaccatgaag catccccatg ggcctcactc ccatcgtggc agacagcaat
     3841 gaagaagcgc tcgtttgtga tcttgctggc tattgcggct gttcccctcc tgttgggagt
     3901 gtcgggcggc acgccatcct cacccggcta ccgttttgcc agacaatctg ccccttacac
     3961 cgagaccgtg gcggcctgga tggcgcaaaa cagtgttgag aaagtcgcgg agactctcac
     4021 ggtctcaccc gcatccgtgg agcaggtgag gtccgttttt cgtctaatgg gacgccgtct
     4081 ggggactctg ccctctacca catcgtcctc ctcatcgggc atcgtgcagc cggtctctgt
     4141 tagttccgag cctttgccgt ccgatcgcga cctggcggaa caacagcctg ctgtggagga
     4201 acgtattcag cggcaggtag cgacggttct tcaccgaagg ggcgtcgggt tcaaggtagg
     4261 cgacggcagc tttctctttc cgccggtgct cttccgcttc agggatcttc cagatctgct
     4321 ggtagtctca cccaggtcgc gcatagagcg gctgggtacg gtgcttctta cacccaacat
     4381 gccattgaca gacgccgtga agttggaagg agaggtgtct tctgggggct actccgcgtt
     4441 ggtgacccac attggtgggc tcggcgtcta ccccagcatg gtaccggaca actccgacct
     4501 tcggtggacg acccgtaccg ttgcccacga gtgggcccat cagttccttg cgcttcggcc
     4561 gttgggatgg cgctacgctt ttggggcgga gcgggatccc aggatggtca tgctaaacga
     4621 gaccgcggcg gaggtgatcg gtcgagag
//
LOCUS       DATALS010000220         3970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_417037, whole genome shotgun sequence.
ACCESSION   DATALS010000220 DATALS010000000
VERSION     DATALS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417037"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2013
                     /locus_tag="VHS06_04850"
     CDS             <1..2013
                     /locus_tag="VHS06_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2987481.1"
                     /translation="NHPVNGPKHCARAEAALQGLYHPDRLQGPMRRTGERGSGQFQPI
                     SWEDGLRELIDRMRGLGAPDQAINLLAITAPLSGHRGLILNRFVSTLGGEQIVFEPFD
                     QATLRSAIKQLFGQDRLPDFEIDKTQYLISFGADFLSTWLSPVRYGAKYGEFRQGQSQ
                     RGTLVQVEPRFSMTAANADVWIPIRPGTEGMLALSMVQVAATEGLVDTAVLQSLTGGA
                     GTGALGPFGPEQVAQTTGVPPERIRQLARDFATKRPSLAIGGGSAAGQTNGLSNLQAI
                     YALNYLTGSVGAPGGIRFNPPPPLPDLSTMAAGGPLTEWQRIAQQLQTSNPKPVDLVM
                     VYGANPVYGLPASLGFGDALLHAGYIVSFSSFMDETTAMADLILPDNHFLESWGDEVP
                     DPGPGYEVVGFQQPVVQPVWDTRDTSNLLLAVADGLGGGLRQALPWESLLEAVQEGAQ
                     QLQLMGRGSVVDANFDQFWVSLLQQGVWWDQRRTAEMPPPSPPALQLQQRSPTFAGSE
                     ADYPFHLVVFPSNSLLGGQVAHLPWLQGLPDPTTTVAWQSWLEINPETSRNLGVNTGD
                     VVRVDSPDGSLRLPAYVHPATPPGIVAVPAGQGHTGFTRYADGRGVNPLKIVSTLTDG
                     DTGSLAWCATRVAIRKTGQRVDLPRFEGTVPAYQLSDAPVVPTVTS"
     gene            2037..2768
                     /locus_tag="VHS06_04855"
     CDS             2037..2768
                     /locus_tag="VHS06_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2987482.1"
                     /translation="MQEQPTPPKHRWGMIVDVDRCIGCQACVVACQAENNVMLNTEDT
                     FTQLRVKEWIRIERYWVGEYPDIKARFLPVLCQQCTNAPCEPVCPVYATSHNEEGLNM
                     QVYNRCVGTRFCGNNCPWQVRFFTFNDPDWPDPLTNQLNPDVTVRSKGIMEKCSYCIQ
                     RIRKAEMKARGEGRELRDMEFQTACAQACPPSALIFGDLNDPNSKVARLQGDRRRYLL
                     LGELGTEPNTIYLKKVDPYPPVQQG"
     gene            3124..>3970
                     /locus_tag="VHS06_04860"
     CDS             3124..>3970
                     /locus_tag="VHS06_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987483.1"
                     /translation="MAEVGRGFAPQEPETEIRSQEVTADVVDLLLTTGSVFRVWAIVL
                     AVLFLAGIGSFVARLLTTGLSDRAGFGYLAATFAFLLATVQAAPILSMGLRLTKANWQ
                     RPLARGSELFAVPAILCSLLYIPLLISLPPIQGRHTLWTVWPGWVLPVFDTIIVVAMV
                     VFGLAFLYLAAIPDFAAARDHIAGRRRGRFALLARGWRGTTSQWTALRFTLILLGAFY
                     AMLYASLQLLISYDFAMTLVPGWLSSIFPAFQITTAFEAAVAVDVLALYIWRRWGGLG
                     RYIRPD"
BASE COUNT          755 a         1284 c         1243 g          688 t
ORIGIN      
        1 aaccacccgg tcaacggccc caagcactgt gcccgcgccg aggccgccct ccagggactg
       61 taccaccccg acaggcttca agggccgatg cgaaggacgg gggagcgggg cagcggacag
      121 tttcagccaa tctcgtggga agacggcttg agggaactca tcgatcggat gcgtggactc
      181 ggagcacctg atcaggccat caacttgcta gcaatcactg cgccgttgag tgggcaccgg
      241 ggactgatcc tcaaccgttt cgtgagcact ctgggtggcg aacagatagt cttcgagccg
      301 ttcgatcagg ccacgctccg ctccgccatc aagcaactgt tcggacagga taggctcccg
      361 gacttcgaga tcgacaagac tcagtacctg atctccttcg gggctgattt cctgagcacc
      421 tggctgtccc ccgtacgata cggggcgaaa tacggcgagt tccgacaggg ccaatcccag
      481 cgaggaaccc tcgtccaggt tgagccgcga ttctcaatga ccgctgccaa cgccgacgtg
      541 tggatcccaa ttcggcccgg caccgaaggg atgctggcac tgagcatggt gcaggtggcg
      601 gcgacggagg ggttggtgga taccgccgtg ctccaatctc tgaccggcgg agccgggacc
      661 ggtgccctcg gcccctttgg tccggagcag gtcgcccaga ccaccggcgt ccccccagag
      721 cgcatcaggc aactggcacg ggacttcgcc acgaaacgac cgagcctcgc catcggcggc
      781 ggatccgcgg cgggccagac caatgggctc tccaacctgc aggcgatcta cgccctcaac
      841 tacctgaccg gcagcgtcgg cgcgcccggc gggatccggt tcaatccccc gcccccgctg
      901 ccggatcttt cgacgatggc cgcaggaggg ccgctcaccg agtggcagcg gatcgcccaa
      961 cagctacaga cctccaatcc taagcctgta gacctggtaa tggtgtacgg ggccaatcca
     1021 gtgtatggac ttccggcatc gctcgggttc ggggacgcgc tgctgcacgc cggctacatc
     1081 gtcagcttct cgagcttcat ggacgagact accgcaatgg ccgacctgat cctgcccgac
     1141 aaccacttcc tagagtcctg gggagacgaa gtgccggatc cagggccggg gtacgaggtg
     1201 gtcggtttcc agcaaccggt ggtccagccg gtgtgggaca cgcgagacac gtccaacctg
     1261 cttctggccg tggccgacgg gcttgggggc ggcctgcgtc aggcactgcc ctgggaaagt
     1321 ctcctagagg ccgtgcagga gggggcacag caactccagc tcatggggcg tgggtccgtg
     1381 gtcgatgcca acttcgacca gttctgggtg tcgctgctcc agcaaggggt gtggtgggac
     1441 cagcgtagga cagcggagat gcctcctccc agcccgccgg cgctccaact acagcagcgc
     1501 tcaccgacct tcgcgggatc cgaggcggac taccccttcc atctcgtggt gttcccgtcc
     1561 aactccctgc tgggcggaca ggtagcgcac ctaccctggc tgcagggctt gcccgatcct
     1621 accactaccg tggcctggca aagctggctg gagatcaacc ccgagacatc tcgaaacctg
     1681 ggcgtgaata ccggcgacgt ggtaagggtg gattccccgg acggctcgct gcgactgccg
     1741 gcctacgtcc accccgccac gcccccgggc atcgtcgccg tccctgccgg ccaggggcac
     1801 accggtttca ctcgctacgc ggatgggcga ggggtcaatc cgctaaagat cgtctccacg
     1861 ctgacggacg gcgataccgg atccctcgcg tggtgcgcga cgcgcgtggc aatcaggaaa
     1921 accggacagc gtgtcgacct tccgaggttc gagggcaccg tcccggcgta tcagctttcc
     1981 gatgcgccgg tagtgcctac agtgacctcg tagctttgtc accctctgga gaagagatgc
     2041 aggaacagcc gacaccgcca aagcacagat ggggaatgat agtcgacgtt gaccgctgca
     2101 tcggctgtca ggcctgcgtc gtggcctgtc aggcggaaaa caacgtcatg ctcaatacag
     2161 aggacacctt cacccagttg cgagtcaagg agtggatccg aatcgagcgc tactgggtcg
     2221 gggagtaccc ggacatcaag gctcgcttcc ttccggtgct gtgccagcaa tgcaccaacg
     2281 cgccctgcga gccggtctgc cccgtctacg caacctctca caatgaggag gggttgaaca
     2341 tgcaggtgta caaccgctgc gtagggaccc gcttctgcgg caacaactgt ccgtggcagg
     2401 tccgcttctt caccttcaac gacccggact ggccggatcc gctcaccaat cagctcaatc
     2461 ccgacgtgac ggtgcgcagc aaggggatca tggagaagtg cagctactgc atacagcgca
     2521 ttcgcaaggc agagatgaag gcgaggggag aggggcgcga gttgcgtgac atggagttcc
     2581 agacggcctg tgcacaagcc tgcccgccga gtgcgctgat cttcggcgac ctgaacgacc
     2641 ccaacagcaa ggtagcccgc ctgcaagggg atcgacgccg ctacctgttg ctgggggaac
     2701 tgggaaccga gccgaacacc atctacctga agaaggtcga cccgtacccg ccagtgcagc
     2761 aagggtgacg aaacatgcac ctgacaatcc agtccgatac ggttcgggcg gatggagtga
     2821 gacaacatga cacgtatcgt agggcggggt cttgtgcccc gcctggagcg tggtcggaat
     2881 caccacggag gtcgtaatca cggtgtggga ggcggggcac aagaccccgc cctacgtcac
     2941 aggataagtg attgcctgca cctaatagag acgctcctta gctccagagc gtgggagaaa
     3001 tcaccgtgga ggtcgcaggc acgccgtggg aggcggggca caagaccccg ccctacgaca
     3061 tggagagggt agaaccctcc agccaacgct tccacagcct aggacagaca gggatgacag
     3121 acagtggcag aagtaggtag ggggtttgcc ccgcaggagc cagaaaccga aatcaggtca
     3181 caggaggtca cggccgacgt cgtggacctg ctgctcacga ccggctcggt tttccgagtc
     3241 tgggcaatcg tgctggccgt cctcttcctg gctggaattg gctccttcgt ggcccgactc
     3301 ctgacaacgg ggttgagtga cagggcaggg ttcggctacc tcgccgccac cttcgcattc
     3361 ctgctggcga ccgtccaggc cgcaccgata ctatccatgg ggctccggct caccaaggcc
     3421 aactggcagc gtccgttagc caggggatcc gaactgttcg cggtcccggc catcctctgt
     3481 tcgcttctgt acatcccact gctaatctcc ctgcccccaa tccaggggcg ccacaccctg
     3541 tggacggtct ggccaggatg ggtactcccg gtcttcgaca ccattatcgt ggtggcaatg
     3601 gtcgtcttcg gcctggcatt cctctacctg gccgctatcc cggacttcgc ggctgcaaga
     3661 gaccacatag ccggcagacg gcgcggcagg ttcgccttgc tggcgcgcgg ttggcgaggc
     3721 acgaccagcc agtggacggc cctacgcttt acgctcatcc tgctgggggc gttctacgcc
     3781 atgctctacg ctagcctgca gctgctgatc tcctacgact tcgccatgac cctggttccg
     3841 ggctggctga gttcgatatt cccggccttt cagatcacca ccgccttcga ggccgccgtc
     3901 gccgtcgacg tgctggccct ctacatctgg agaagatggg gaggactggg tcgctacatc
     3961 cgtcccgacg
//
LOCUS       DATALS010000221         7032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_157297, whole genome shotgun sequence.
ACCESSION   DATALS010000221 DATALS010000000
VERSION     DATALS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157297"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VHS06_04865"
     CDS             complement(<1..802)
                     /locus_tag="VHS06_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX2987484.1"
                     /translation="MRLKEYEGKRILQSMGVALPKGKLVETPAQARAAAQEIGGPVVL
                     KVQVLATGRGKVGGVRFADTPEQAEQVAAQMLGMQIKGYTVQQLLVEEKLDVAQEIYV
                     AATFEEATKGRLMMASASGGMDINEVAEKDPNLIKRVHMDAWIDMHPFQAKELAFGLG
                     LTGKVNSAAQSLFLKLWATFVRFDAILAEINPVVVAKDGRVVAADAHIEIEGDSMFRQ
                     KDRLAALEIYDRDDNAKPPTPFEIEALKIDKADYRGVAGRVVEFPGSLG"
     gene            complement(990..1541)
                     /locus_tag="VHS06_04870"
     CDS             complement(990..1541)
                     /locus_tag="VHS06_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX2987485.1"
                     /translation="MANRREVRISGFGGQGIVLAGVILARAAAIYNNTEATQSESHGA
                     ESRGGACLSDVVLSDERVDYPMCLSPEVLVCMSPEAANRYVPTVKPGGLFIIDEDLVP
                     EPPKGQYSKMLKIPATRVAEEQFKRVMANVVMLGALIGIDPLVKRESMEKAVLASAPK
                     GTEDMNLAALRKGFELGEAALKA"
     gene            complement(1534..2406)
                     /locus_tag="VHS06_04875"
     CDS             complement(1534..2406)
                     /locus_tag="VHS06_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013099507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2987486.1"
                     /translation="MADAPVIATAESEALNHPAMKYFRHGAGGNPHIWCQGCGIGQIW
                     YYTATAIDELGLDPNTVVWVGGSGCTGRMCTYWKYDYMHTLHGRPLGFATGIKLANPD
                     LNVICHMGDGETSAIGGNHLIQTARRNANVTAICVNNYTYGMTGGQYSPTTPMGSISA
                     TSRYGLVEDGFDLCQLVAAAGATYVARTTTSNPRPSINMIKKAITHKGFSFVEIISQC
                     PTYYGRSNKLGNNLEMLDWMKRTYVRKEQFDTMTAEERVGKKPWGEFVDTERPEVVEG
                     LHKLAKAQLEAYRG"
     gene            complement(2407..3555)
                     /locus_tag="VHS06_04880"
     CDS             complement(2407..3555)
                     /locus_tag="VHS06_04880"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX2987487.1"
                     /translation="MANPRLLTGVHLMQGQQACAEGALAAGCNLFAGYPISPATEIAE
                     AMARRMPQAGGIYLQGADELDSLTIVIGSVWGGYKSMTATSGNGICLMQENIGYAAMT
                     ETPCVIVDMMRAGPGTGIATKSAQGDIYQVRYGSNGDYSVIALAPESPQEMFDLTVEA
                     FNLSEQYRVPVFVLGDEILSNLRERVVIPSEVKLTPRIKPTEKPAEGYKTWAIPAGKD
                     VAPLPAFGQGYHLAVSSFARTPEGAPSQAYAVQKEMASRLWNKIEMHSDELTRVEDGF
                     LDDAEVLVIGFGSVARSVKAGVRQARAEGLKVGFSRLIGIWPFPDNYIARIGKNVKKV
                     IVAEMNMGKVSREVQRCLGRDTEVILSSKPGVLMHTPAEILDEIRKVI"
     gene            complement(3555..3809)
                     /locus_tag="VHS06_04885"
     CDS             complement(3555..3809)
                     /locus_tag="VHS06_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX2987488.1"
                     /translation="MAPKAKTGHRIAVNHNYCTGCGNCVKFCPMHVLETDTKLNKRGV
                     HAPVVAAIEKCTGCQLCEMYCGNFAIAVGSVPSADEEGVV"
     gene            complement(3954..4643)
                     /locus_tag="VHS06_04890"
     CDS             complement(3954..4643)
                     /locus_tag="VHS06_04890"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2987489.1"
                     /translation="MAKPVLLANYPDLGERVYEAVREQIVTCELAPGSPLPVVDLARQ
                     FGVSLTPVRDALNRLAAEGLVEDVARKGYFVARLDPEDIADLLSARRLIELAAVEEGI
                     GRIQSDQLVELKRLFEEMRQLFDGNGNCLDYAEFSRRDSQFHQLVVGTAGNEHLVHIY
                     RSLSVHVHIYRTNLAAQGGYRRSLAGVREHEAILVAFESRDLPALKAAISAHVRGVLA
                     EFSGNLRSDPQ"
     gene            complement(5184..5807)
                     /locus_tag="VHS06_04895"
     CDS             complement(5184..5807)
                     /locus_tag="VHS06_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX2987490.1"
                     /translation="MARLDGSTIAIVVAHEFEDIELLYPIIRLGEEGAQMIVATLPLE
                     TPGHFHTRPYAPDKPVTGRFGATVPLVVMEEGKHHTHRLVTELKPSDFDALMFPGGFS
                     PDFLRIHQPTLQLVADAYAAGKVIAAICHGPQVLISADRRLGTDLVRGRRVTGYVAIE
                     DDLRNAGGEFVDVGAVRAGNVVTGRCPDDLAAFCHEITEALVAARQG"
     gene            complement(5825..6895)
                     /locus_tag="VHS06_04900"
     CDS             complement(5825..6895)
                     /locus_tag="VHS06_04900"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEX2987491.1"
                     /translation="MRAAVWYGRRDTRVVDVPDAPSPGPGEVKIKVAWVGVCGTDLHE
                     YAAGPIFMPIEPHPLTGKAAPLIQGHEFSGVIVEVGAGVGGFKPGDRVTADSATWCGE
                     CWACQRREYSLCDKAAFLGLGRDGAFAEYITIPTGALFHLPPSLSLEKASFCEPTAVA
                     LHALRKGRMTPGDSVLVIGAGNQGLLAWQILKHSGAHQVFVAARKGARASVARQLGAE
                     VLDPTEIDVAAEVRARTGGNGVDLTIEAAGRQETVAMALRATRKLGRIVEVGIFEEPI
                     SVDLNDLVFHERQWLGIMNNGGEFPQAIQMLADGRVDPTPMISNRISLEEVVERGLEE
                     CVVNKRANVKVIVNCNADLRDL"
BASE COUNT         1284 a         2305 c         2076 g         1367 t
ORIGIN      
        1 gtcccagaga accggggaat tcgacgacgc ggcccgcaac gccacggtaa tccgccttgt
       61 cgatcttcag agcctcaatc tcgaacggtg tcgggggctt ggcattatcg tcgcggtcgt
      121 agatctccag ggccgccagc ctatccttct ggcggaacat ggagtcgccc tcgatctcta
      181 tgtgcgcgtc ggcggccacc acgcggccgt ccttggccac gacaacgggg ttgatctcag
      241 ccaaaattgc gtcgaaacga acaaacgtag cccacagctt gaggaagagt gactgcgcgg
      301 cagagttgac cttgccggtg aggcccagcc caaacgccaa ctccttggcc tggaaggggt
      361 gcatatcgat ccaggcatcc atgtgcaccc gcttgatgag gttaggatcc ttctcggcga
      421 cctcgttgat atccataccg ccggaggcgc tggccatcat caaccggccc ttcgtcgcct
      481 cttcgaacgt tgccgctaca tagatctcct gggcaacatc cagcttctct tccaccagca
      541 gttgctgaac ggtgtagccc ttgatctgca tgcccagcat ctgggccgca acctgctccg
      601 cctgctccgg ggtatcggca aacctgaccc caccgacctt gccgcggccg gtagccagaa
      661 cctgaacctt caacacgacc gggccgccga tctcttgcgc cgcggccctt gcctgggccg
      721 gagtctctac cagcttgccc ttggggagtg ccacccccat gctctgcaag atgcgcttgc
      781 cttcgtattc cttcagtctc atccgcgtcc tccagtagga tgggatactg atcccaccta
      841 ccatcatgat gggtcaacta ctctatcacg tggaacccct ctccacaggg agagggtcgg
      901 ggtgaggaag aagggaaggt agaaacagcg ccgttgccgt cccccgccct tcgggcgggg
      961 gactaagccc ccgccctacg atgtcgatac tacgccttca gggcagcctc gcccaactcg
     1021 aaacccttgc ggagggcagc caggttcata tcctcggtgc ccttaggagc gctggccaga
     1081 acggccttct ccatggattc ccgcttgacc agcgggtcga tgccgatgag agcgcccagc
     1141 atgaccacgt tggccataac ccgcttgaac tgctcctctg ctacgcgggt cgccggtatc
     1201 ttcagcatct tggagtactg ccccttgggg ggctcgggaa ccaggtcctc gtcgatgatg
     1261 aacagcccac cgggcttgac ggtcgggacg taccggtttg cagcctcggg ggacatgcac
     1321 accaggactt ccggggacag gcacatcggg tagtctaccc gctcgtcgga gagcaccacg
     1381 tcggagaggc aggcgccgcc gcgagactcc gcaccgtggg actcagactg ggtcgcttcg
     1441 gtattgttgt agatggcggc ggccctggcc aggatgaccc cagccagcac gatgccctgg
     1501 ccgccgaagc cgctgattcg aacctctctt ctgttagcca cggtaagcct ccagctgagc
     1561 cttggcgagc ttatgcagcc cctcgaccac ctcaggccgc tcggtgtcga cgaactcgcc
     1621 ccaaggcttc ttgccgaccc gctcctcggc ggtcatcgtg tcgaactgct ccttgcgaac
     1681 gtaggtcctc ttcatccagt ccagcatctc caggttgttg cccagtttgt tggaccggcc
     1741 gtagtaggtg gggcactggg agatgatctc tacgaagctg aagcccttgt gcgtgatcgc
     1801 cttcttgatc atgttgatgg agggcctagg attgctggtg gtggtgcggg caacgtaggt
     1861 tgcgcccgcg gccgctacca actggcacag gtcgaaaccg tcttccacca gaccgtagcg
     1921 tgaggtcgcg gaaatcgacc ccatcggggt ggtgggggaa tactggccac cggtcatgcc
     1981 gtaggtgtag ttgttgacgc agatggcggt gacgttggcg ttccgccggg cagtctggat
     2041 caggtggttg ccaccgatag cggacgtctc gccatcaccc atgtggcaga tcacgttcag
     2101 gtccgggttt gccagcttga tgccggtagc gaagcccaga ggccgaccgt gcagggtgtg
     2161 catgtagtcg tatttccagt aggtgcacat tcggccggtg cagccgctac cgcccaccca
     2221 cactaccgta ttggggtcga gtcccagctc gtcgatggcc gtggcagtgt agtaccaaat
     2281 ctggccgatt ccacagccct gacaccagat gtgagggtta ccgccggcgc cgtggcggaa
     2341 gtacttcatg gcggggtggt tgagcgcctc ggactctgca gttgcgatca caggtgcatc
     2401 tgccacttag atcaccttcc ttatctcgtc caggatctcc gccggcgtgt gcatgagaac
     2461 accaggcttc gaggacagga tcacctcggt gtctcggccg aggcagcgct ggacctcgcg
     2521 cgacaccttg cccatgttca tctcggcgac gatcaccttc ttgacgttct tgcctatgcg
     2581 cgcgatgtag ttgtcgggga aaggccagat cccaatcaac cggctgaagc cgaccttcag
     2641 tccctctgcc cgagcctgcc ggacgcccgc cttgacggag cgagcgacgg agccgaagcc
     2701 gatgaccagc acttcggcgt catccaggaa gccgtcctcg acgcgggtca actcgtcgga
     2761 gtgcatctcg atcttgttcc agagcctgct ggccatttcc ttttggacgg cgtaggcctg
     2821 ggaaggagca ccctcggggg tgcgagcgaa gctggacacg gccaggtgat atccctgccc
     2881 gaaggccggc agcggcgcga cgtccttgcc tgccgggatt gcccaggtct tgtagccctc
     2941 agccggcttc tcagtcggct tgatgcgcgg ggtcagcttc acttcggagg ggatgacgac
     3001 gcgctcgcga aggttggaaa ggatctcgtc acccagaacg aagaccggga ctcggtactg
     3061 ctcggagagg ttgaacgcct cgaccgtgag gtcgaacatc tcctgcgggg actcgggagc
     3121 cagggctatc acggagtagt caccgttgct gccgtagcga acctggtaga tatcgccctg
     3181 agcgctcttg gtggcgatgc cggtgccggg tcctgcccgc atcatgtcca cgatcacgca
     3241 gggggtctcg gtcatggccg cgtagccgat gttctcctgc atcaggcaga tgccgttgcc
     3301 ggaggtagcg gtcatagact tgtagccgcc ccagaccgaa ccgataacga tagtcaggga
     3361 gtcaagctcg tcggcgccct gcagataaat gccgcctgcc tggggcattc gccgagccat
     3421 tgcctcggcg atctcggttg ccgggctgat gggatagccc gcgaagaggt tgcagccggc
     3481 agccagagcg ccctcggcgc aggcctgctg cccctgcatc agatgcacgc cggttagaag
     3541 tctcggattc gccatcagac tacaccttcc tcgtctgcac tcggcacgct gcccacagca
     3601 atggcgaagt tgccacagta catctcgcac agctggcagc cggtgcactt ctcaatggcc
     3661 gcgaccaccg gcgcatggac gccccgctta ttcagcttgg tgtccgtctc cagcacgtgc
     3721 attgggcaga acttcacgca gttgccgcag cccgtgcagt agttgtggtt caccgcgatt
     3781 cggtgcccag tctttgcctt tggagccacg ttgttcctcc cttacctgaa cactagcagt
     3841 ggaagactac gatcaagtta ctatagtgcg ccaccccctg accctcccgg gcgagaaggt
     3901 ggcgcaaagg acccgcaaag aaaatggtaa agtgcccgat atggtattgc ggtctactgt
     3961 ggatcgctac gcaaattccc gctgaactcc gccagcactc cacgaacgtg ggcactgata
     4021 gcggccttca gcgcgggcag gtcgcgagat tcgaaggcga ccaggatggc ctcatgctcg
     4081 cgcacgccgg cgagactgcg acgatagcca ccctgagccg cgagattagt cctgtagatg
     4141 tgcacgtgca cgctgagact gcggtaaatg tgaacgaggt gctcgtttcc ggcggtgccg
     4201 actaccaact ggtggaactg cgaatcgcgc cggctgaact cggcatagtc tagacagttg
     4261 ccattgccat cgaacagttg acgcatctct tcgaagagcc gcttcagctc cacgagttga
     4321 tcggactgga tccggccgat cccttcttcc accgcggcca actctatgag ccggcgggcg
     4381 ctcagtaaat cggctatgtc ctcgggatcc aacctggcca cgaagtagcc cttgcgagct
     4441 acatcctcca ccaacccctc ggcagccagg cgattcagcg cgtcccgaac cggagtgagg
     4501 ctgacgccga actggcgagc cagatccaca accgggagtg ggctgcctgg tgcgagctcg
     4561 caggtcacaa tctgctcgcg aacggcctcg tacaccctct cgccgagatc tggatagttg
     4621 gccagcagca ccggtttcgc catgaaaaac gcctcatccg agtagcgtga tcgcagggtt
     4681 ggtcgccagc ctaccatacg ccgcacatca tacgggatgc tcgatatgcg gcacacgata
     4741 tctgatatat ttggcgacga cacggatgtt atacgcctgc tccgtagggc tgtcaagaag
     4801 attctgttac gacccggttt cgatctagaa gggggaaagg caaggcgtct gcgggcacgg
     4861 agggattcgt cggtatggcc caaccgacgc cccgccggtt ggtcgcgtgg cctgtaggag
     4921 ccgcctctgg cgtctcgtag ggcggggtct tgtgccccgc ccagagcgtg attggtacca
     4981 cttatgtggt cgtggccaag gtgcgagagg cggggcacaa gaccccgccc tacgtctcca
     5041 tcaaccctgg tcaaacctcg ttgcagaacc gaatgccgct tgcggcgggg aacagacgtg
     5101 acgctacgct cgctcgcgcc ccccgcccta cgtcacgttc caaccgtgcc actgcctcaa
     5161 gggtagggca ccgtcccgag ccgtcagccc tgcctggccg ccaccagcgc ctccgtgatc
     5221 tcgtggcaga aggccgccag gtcgtcagga cagcgtccgg tcacgacgtt ccccgccctc
     5281 actgccccga cgtccacgaa ctcccctccc gcgttccgca gatcgtcctc tatggcaacg
     5341 tatccggtca cacggcggcc tcgaaccaga tccgtgccga gtcgacggtc ggctgaaatc
     5401 agcacctgcg ggccgtggca gatagcggcg atgaccttgc cggccgcgta tgcatccgcg
     5461 accaactgca aggttggctg atggatgcgc agaaagtccg gggagaagcc gcccgggaac
     5521 atcagcgcgt cgaaatcgga cggcttcaac tcggttacca gcctgtgagt gtggtgcttc
     5581 ccctcctcca tcaccaccag cggaaccgtc gccccaaagc gtccggtaac cggcttgtcg
     5641 ggcgcgtagg gtcgagtgtg gaaatggccg ggtgtctcga ggggcaacgt cgccacgatc
     5701 atctgggcgc cctcttcgcc aagccgaatg atcgggtaga ggagttcgat gtcctcgaac
     5761 tcgtgggcca ctactatcgc aatcgtagat ccgtccaacc tggccacggt gagaacctcc
     5821 tccctcacag gtcgcggagg tcagcgttac agttgacgat gaccttcaca ttcgcacgct
     5881 tgttgacgac gcactcctcg agtcccctct ccaccacctc ctccagggag atccggttgc
     5941 tgatcatcgg cgttggatcg acccgcccgt cggccagcat ctggatcgcc tgtgggaact
     6001 caccgccgtt gttcatgatc cccagccatt gtctctcgtg gaacaccagg tcgttcaggt
     6061 cgacggaaat cggctcctcg aagatgccaa cttcgacgat gcggcccagc ttccgggtcg
     6121 cccgcaacgc catggccacc gtctcctgcc tccccgccgc ctcgatggtg aggtcgacgc
     6181 cgttccctcc cgtgcgagcc cgcacctctg ccgccacgtc gatctccgtt ggatccagca
     6241 cctccgcacc caactgcctg gccaccgaag ccctggcccc cttccgtgcg gcgacaaaga
     6301 cctggtgcgc tccagagtgc ttcagtatct gccaggccag cagcccctgg ttcccagcgc
     6361 cgatgaccag cacgctgtca ccgggggtca tccttccctt cctcaaggca tggagggcaa
     6421 ccgcggttgg ctcgcagaag gatgccttct ccaacgagag gctgggaggc aggtggaaga
     6481 gagcgcccgt gggtatcgtg atgtactcgg cgaaagctcc gtcgcgcccg agccccaaaa
     6541 acgcggcctt gtcgcaaaga ctgtactcgc ggcgttgaca agcccagcac tccccgcacc
     6601 aggttgcgga gtctgcggtt acccggtcgc ccggcttgaa gccgcccaca ccagcgccga
     6661 cttcgacgat caccccgctg aactcatgtc cttgaatcag cggcgcggcc ttgccggtca
     6721 gagggtgggg ctcgataggc atgaagatcg ggcccgccgc atactcgtga aggtctgttc
     6781 cgcacacccc cacccaggca accttgatct tgacctcacc aggcccaggg ctgggcgcat
     6841 cgggcacatc gacgactcgc gtgtcccgcc taccgtacca gacagcagcc cgcatcggtc
     6901 ttctcctttc cgctcatcac tcgtcaaacc attgctgccg ccaacacagt tgcttctaac
     6961 ttcttgttgc tgcctatact agatttattt ttctaccacg tcaaggcact cttctacagt
     7021 atcgttcgca gc
//
LOCUS       DATALS010000222         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_23465,
            whole genome shotgun sequence.
ACCESSION   DATALS010000222 DATALS010000000
VERSION     DATALS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23465"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1959)
                     /locus_tag="VHS06_04905"
     CDS             complement(103..1959)
                     /locus_tag="VHS06_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX2987492.1"
                     /translation="MAAVFKFGGYAGQLLRVNLTTKVVSKEPLREDWARDFVGGVGYA
                     ARLLYDELASKIDPLGPENKLVFMTGPTCGTLIPSGSRGCVCGKSPATGSFFHSIYGG
                     YWAAELKFAGYDGVILEGKSDKPVYLWIDDDKIELRSAEGIWGKSTVDAEMAIRHELG
                     DDEIQIATIGKAGELGTPYAMILVGIRAAGRGGLGAVMGSKMLKAVAVRGSGNLHVPN
                     MLNMYNTSMKLCEALEAPTKGLLEYGTARNSGPLNAAGILPTHNWQTEVFSGIDGITG
                     ETMKKEVVKANRSCFACSVNCTKYSVVPSGPYKSVVEGPDYETLFGYGSMCEVGDIRA
                     SCKADELCDEYGLDLMSASCTIAWAMECYEKGIFTKEDTGGIDLRFGNAEAMIKMTEM
                     IGKREGLGALLAEGVREASKIVGKGSERFAVHVKGLELAGHTPRPFPATAVGYATGPR
                     GGSHHDSRTTMEKFGMVERKTLEGKGALAAEVNHFLIFTDSVILCHLPETFWGPSGIK
                     ETVVEALNAVTGWKLDVEQAHKIAERQWNMIRCFCAREGFTRKDDQLPVRMMEEPIPE
                     GPMKGEVMPLETLERLKNDYYSYRGWDLESGNPTREKLTELGLEFAIKDVCP"
     gene            complement(1959..2249)
                     /locus_tag="VHS06_04910"
     CDS             complement(1959..2249)
                     /locus_tag="VHS06_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX2987493.1"
                     /translation="MAKVRVRGFTVIRDIFQSEVVEVDVPRPETVKELFAALLRRYGS
                     PLKEIICDPKSGELSSFPVRLNDEMLTSVRDGDRRVSNGDELTIIFPIGGGC"
     gene            complement(2257..>2571)
                     /locus_tag="VHS06_04915"
     CDS             complement(2257..>2571)
                     /locus_tag="VHS06_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987494.1"
                     /translation="VEKHIEIHTELCTGCKLCELACSAVKTGEFNPREARIKVSLVNI
                     PEIPVPTILDSCDYCFGNPVCVRFCNPKAIVWKEMESKPVRSKVSEAKQIAESWLESV
                     SK"
BASE COUNT          444 a          883 c          738 g          506 t
ORIGIN      
        1 gcggggcaca agaccccgcc ctacgttgcg atcacgccgt atctctaggc ctggtcagta
       61 gaagagatgc gccgggtcag ggacgacccg gccctcctga tatcacgggc acacgtcctt
      121 gatggcgaac tccagcccca actccgtgag cttctccctg gtggggttgc cgctctccag
      181 gtcccagccg cggtaggagt agtagtcgtt cttcagccgc tccagcgtct ccagcggcat
      241 cacctcaccc ttcatcggcc cctcggggat cggctcctcc atcatgcgga cgggcagctg
      301 gtcgtccttg cgggtgaagc cctcgcgagc gcagaagcag cggatcatgt tccactgccg
      361 ctcggcgatc ttgtgggcct gctccacatc cagcttccag ccggtgaccg cgttcagcgc
      421 ctcgaccacc gtctccttga tcccgctggg cccccagaag gtctcgggca ggtggcagag
      481 gatcaccgag tcggtgaaga tcaggaagtg gttcacctcg gcagccagcg cacccttgcc
      541 ctccaacgtc ttccgctcca ccatcccgaa cttctccatc gtggtgcggg agtcgtggtg
      601 gctacctccc ctcgggccgg tggcatagcc cacggcagtc gcggggaagg gtctcggggt
      661 gtggcccgcc aactccagcc ccttgacgtg aacggcgaag cgctcgctgc ccttgcccac
      721 gatcttggag gcctccctga cgccctcggc cagcagagca cccaggccct ctcgcttgcc
      781 gatcatctcg gtcatcttga tcatggcttc cgcgttgccg aagcgcaggt cgatcccacc
      841 ggtgtcttcc ttggtgaaga tccccttctc gtagcactcc atcgcccagg cgatggtgca
      901 ggaggcggac atcagatcca gcccatactc gtcgcacagc tcgtcggcct tgcagctcgc
      961 ccggatgtcg cctacctcgc acatgctgcc gtagccgaag agggtctcgt agtccgggcc
     1021 ctcgaccacc gacttgtagg ggccactggg gacgacgctg tacttggtgc agttgacgga
     1081 gcacgcgaag caggaacggt tggccttcac cacctccttc ttcatcgtct cgcccgtgat
     1141 cccgtcgatg ccggagaaca cctcggtctg ccagttgtgg gtggggagga tccccgcggc
     1201 gttcaacgga ccggagttcc gagccgtgcc gtactccagc agccccttgg tgggggcctc
     1261 gagcgcctcg cacagcttca tggaggtgtt gtacatattg agcatgttgg ggacgtgcag
     1321 gttgccggag cccctcaccg cgaccgcctt cagcatcttg gagcccatga cggcgcccag
     1381 cccgccgcgg ccggccgccc tgatccccac caagatcatc gcgtaggggg ttcctaactc
     1441 gccggccttt ccgatggtgg cgatctggat ctcgtcgtca cccagctcat ggcggatcgc
     1501 catctccgcg tccacggtgc tcttgcccca gataccctcg gcgctcctca gttcgatctt
     1561 gtcgtcgtcg atccagaggt agaccggctt atccgacttg ccctcgagga tcacgccgtc
     1621 gtacccggca aacttcagct ccgcggccca atagccaccg tagatgctgt ggaagaagga
     1681 gccggtcgcc ggcgacttgc cgcagacgca gcccctggag ccggacggaa tcaacgtccc
     1741 gcaggtgggg ccggtcataa acaccagctt gttctcggga cccaggggat cgatcttcga
     1801 cgccaactcg tcgtagagca ggcgtgcggc gtaccccaca ccgccgacga aatctctggc
     1861 ccagtcctcc ctgagaggct ctttggagac caccttcgtg gtcaggttca ccctgagcag
     1921 ttggcccgcg tagccgccaa acttgaatac cgcagccatt agcaacctcc cccgatcgga
     1981 aagatgatgg tcaactcatc accattacta acgcgcctgt ccccatcccg gacagacgta
     2041 agcatctcgt cgttgagtcg aaccggaaag ctggatagct ccccgctctt tgggtcgcag
     2101 atgatctcct tcagcgggct tccatatcgc cgaagcagcg cggcgaacag ttccttcacc
     2161 gtctcgggtc gagggacatc gacctcgacc acctcggact ggaagatgtc ccgaattacc
     2221 gtgaagcccc tgactcgcac ctttgccaca tcacacctac ttactcaccg actccagcca
     2281 agactcggca atctgcttcg cctctgagac cttcgaccgg acgggcttgc tctccatctc
     2341 tttccagacg atcgccttgg ggttgcagaa gcggacacac acggggttgc cgaagcagta
     2401 gtcgcaggaa tccaggattg tgggaacggg gatctcggga atgttgacca ggctcacctt
     2461 gatcctggcc tcgcgcgggt tgaactctcc agtcttgacg gcagagcagg caagctcgca
     2521 cagcttgcag ccggtgcaca gttccgtgtg gatctcgatg tgtttttcca c
//
LOCUS       DATALS010000223         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_380295, whole genome shotgun sequence.
ACCESSION   DATALS010000223 DATALS010000000
VERSION     DATALS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380295"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..895
                     /locus_tag="VHS06_04920"
     CDS             194..895
                     /locus_tag="VHS06_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003961350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2987495.1"
                     /translation="MGRRLLVADDDRKTVELVKLYLERDGYQVVTAHDGRTALELARG
                     QHPDLVVLDLMMPELDGLDVCRLLRLESKVPIIMLTARTTEGDRITGLDLGADDYVTK
                     PFSPRELVARVRAVLRRIAEQEAEEGPQQVQIGSVLVDFRKHEAKADEEPIQLTPTEF
                     KLLKVLAEEPGRAYSRAQLVDRVLGNGYEGFERTIDVHVMNLRRKIEPDPSHPQYLLT
                     AYGVGYKLNENRHDS"
     gene            885..>1610
                     /locus_tag="VHS06_04925"
     CDS             885..>1610
                     /locus_tag="VHS06_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX2987496.1"
                     /translation="MIRSIRLRLLLAFMLVLGVALSGMSVLTSRSTATEFHGFMENRS
                     ANNFRRVMEGIVRYYGDNQGWTGIQGFVERTSQLTGDHIMVADNSGSIVADSDSAMVG
                     QPANQPAGVPVIVAGTQVGTAYVNPGQSTPQLPPPEKGPPPEIAAAAFVSSVNRSILM
                     SAVAAALLALLLTVILSRRILRPVEALTSAARAMEKGDLAQRVTIKTHDEVGDLAEAF
                     NSMAESLAKTERLRQNMVTDVAHE"
BASE COUNT          351 a          491 c          494 g          274 t
ORIGIN      
        1 tgcgtcgccc ctacactgtg ggttccgggc tcgcccgcac cgcagccaag atccccaaaa
       61 cttcataacc ccctgacacg ttcttaacat gatctttaca atccctccgt tatcataccc
      121 aggatggcac aggatcgccg aagccgtctg ctatcatgtt atccatcagc ggagtttcga
      181 aggagtcagg gcaatgggca ggagattact ggtcgccgac gacgacagga aaactgtgga
      241 gcttgtgaag ctgtacctgg agagggacgg ctaccaggtc gtaaccgccc acgacggtag
      301 gacggctctg gaactggctc gggggcaaca cccggacctg gtagtgctgg acctcatgat
      361 gccggaactg gacggtctgg acgtatgccg cctgctccgg ttggagtcca aggtaccgat
      421 tatcatgctc accgcgagga caaccgaggg ggaccggatc accggcctgg acctgggagc
      481 agacgactac gtgacgaagc ctttcagccc cagggaactg gtcgcccggg tgcgagcggt
      541 gcttcgaagg attgccgagc aggaggccga ggagggccca cagcaggtcc agattggatc
      601 cgtcttggtg gacttccgca agcatgaagc gaaggcagac gaagaaccca tccagctcac
      661 gcctacggag ttcaaattgc tgaaggtgct ggctgaagag ccgggtcgag cctacagtcg
      721 agcccaactg gtcgatcgag tgctcggcaa cggctacgag ggcttcgagc gaaccatcga
      781 cgtccacgta atgaacctgc gcaggaagat cgagccagat ccttcccatc cccaatacct
      841 gctgacagcc tacggcgtgg gctacaagct gaacgagaac cgccatgatt cgtagtatcc
      901 ggttacggct gctgctggcc ttcatgctgg tgctgggcgt cgccctgagc ggaatgtcgg
      961 tgctgaccag ccgaagcacc gcgaccgagt tccacggctt catggaaaac cgcagtgcca
     1021 acaacttccg gcgcgtcatg gagggaatag tccggtacta cggcgacaac cagggatgga
     1081 cggggatcca ggggttcgtg gagcgaacgt cgcaactgac gggcgaccat ataatggtcg
     1141 cagacaacag cggcagcatc gtcgccgact ccgatagtgc catggtcggg cagccggcca
     1201 atcaaccggc aggtgtgccc gtcatcgtcg ccggtactca ggtgggaacc gcctatgtga
     1261 accccggcca gagtacacca cagttaccgc caccggagaa agggccacca cccgagatag
     1321 ccgccgctgc cttcgtctcg tcggtcaatc gctcgattct aatgtctgcc gtggctgcag
     1381 cattgctggc actgcttctg accgtgatcc tctcgaggcg catcctccgc cccgtcgagg
     1441 cgctcacctc cgcggcgcga gcgatggaga agggggacct cgcccaacga gtcaccatca
     1501 agacccacga cgaagtgggg gatctcgcgg aggcgttcaa ctccatggca gagagtctcg
     1561 cgaagaccga gagactgcgc cagaacatgg tcaccgacgt cgcccacgag
//
LOCUS       DATALS010000224         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_247799, whole genome shotgun sequence.
ACCESSION   DATALS010000224 DATALS010000000
VERSION     DATALS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247799"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..497
                     /locus_tag="VHS06_04930"
     CDS             333..497
                     /locus_tag="VHS06_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987497.1"
                     /translation="MPENNHVNQTALVKVVESVDIQLCEQLKARAGGDEALLGKLLAQ
                     VIETWLAEDH"
     gene            522..1217
                     /locus_tag="VHS06_04935"
     CDS             522..1217
                     /locus_tag="VHS06_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX2987498.1"
                     /translation="MTRVPLILPATPSISWRVIHSARKPSSHFSLQQVMDTLSPLAQP
                     NRWIEAADPLRSSREPKGARRVQAIAGVAEGLMPSQFDASISSTEANAACGPAAAVAF
                     ARASGKNVSLRQAVDLARTVGWTVSGGMNGVVNQKRLLDKMGIPALLDTSGDWSKVAR
                     TVSAGSPVTISTPGHYFVADDYDPKTGRFHVGASGTAYQGGKEWMTRQEMEGAAGRIN
                     GTLVMKDRSRWMA"
     gene            complement(1214..1378)
                     /locus_tag="VHS06_04940"
     CDS             complement(1214..1378)
                     /locus_tag="VHS06_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987499.1"
                     /translation="MPENNHVNQTALVKVVESVDVQLCEQLKARAGGDEALLGKLLAQ
                     VIETWLAEDH"
     gene            1841..2644
                     /locus_tag="VHS06_04945"
     CDS             1841..2644
                     /locus_tag="VHS06_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987500.1"
                     /translation="MPDSSALSSSARRKREVMFVPQAEQGPVCHFAVDDAALIRFLRD
                     NCEALAPVISSITELRRPVNRSLGEAVARLLVVTDEPIQAAFVSRASNAIARMVSVLS
                     PSTLGEAVGESSDYEVLLDALEAQEVVTALQADDPLAAARLRGIQARNELLTAEGGVL
                     GVGEVAELLGITRQAVDKRRRNGTLLGLAKGQSKYAYPAWQFAGHRVLPGFEEVLACF
                     KVEDPWTRAGFFLSGNALLNGQRPLDVLRQGHIDQVCRAAGAFGEHASW"
     gene            2745..3290
                     /locus_tag="VHS06_04950"
     CDS             2745..3290
                     /locus_tag="VHS06_04950"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX2987501.1"
                     /translation="MTIRWYRLAANPRTPVYWGLTPPRIPSARFDAPKGEFGVLYAGV
                     DAYCAFIETYGHVTGTRWVTLAALRERSLFEIRFGHPLRLVNLTGHHLAGLGADSGLF
                     SGDYTVSQAWSLAFFNHPSAPDGLYYHTCHDPSRMSLALYQRGDIESQVSFTSLGTLA
                     DSGNRALLGKILDTYRFALLP"
BASE COUNT          688 a         1083 c         1089 g          741 t
ORIGIN      
        1 cggcacgatg agagcggttt cctcgttgac ctgtttggtc ccgttggggt cgccgtgggc
       61 aggtagcccc tccttctcat tggagaacca tgcgtgacag cagaaagagc cgcacatgac
      121 gtcgggcacc gatgccgatt cagatcgccc caagggccgc gaactgggcc acaagttttc
      181 ggcgattctg ccgatcctaa taagtgtagg tggagtgtgc ccacgaaggg atcgggcggt
      241 aaggcatcca ctgtcacgag ggccggggat cgatcagaga gagaacgtgc gtcgatccct
      301 aggctactga aagcgagcag aggatgggaa agatgccgga gaacaaccac gtcaaccaga
      361 ccgcgctggt gaaggtcgtc gagagtgtgg acatccagct ctgtgagcag ctcaaagcta
      421 gagcgggggg agacgaggcg ctgctgggca agctgctggc tcaggtgata gagacgtggc
      481 tggcagagga ccactaggcc atccagcgag acctgtcctt catgaccagg gtgccgttga
      541 tcctgcccgc caccccttcc atctcctggc gggtcatcca ctccgcgcga aaaccctctt
      601 ctcacttctc attgcagcag gttatggata cgctgtctcc cctggcccag ccgaaccgat
      661 ggatcgaggc cgccgatccc ttgagaagca gccgcgagcc caagggcgcc agacgcgtcc
      721 aggcgatcgc cggggtggct gaggggctga tgcccagcca gttcgatgcc tccatctctt
      781 ccactgaggc gaacgctgcc tgcggccccg ccgcggcggt ggcctttgcg cgagccagcg
      841 gcaagaacgt gtcgctgcgc caggcggtgg atctcgcacg gaccgtcggg tggacggtct
      901 ccgggggaat gaacggagtc gtcaaccaga agcggttgct ggataagatg gggatccctg
      961 cccttctgga cacctccggc gattggagca aggttgcccg caccgtgtcc gccggcagcc
     1021 cggtcaccat cagcaccccg ggacactact tcgtggccga cgactacgac ccgaagaccg
     1081 ggcgcttcca cgttggggca agcggaaccg cctaccaggg gggcaaggag tggatgaccc
     1141 gccaggagat ggaaggggcg gcgggcagga tcaacggcac cctggtcatg aaggacaggt
     1201 ctcgctggat ggcctagtgg tcctctgcca gccacgtctc tatcacctga gccagcagct
     1261 tgcccagcag cgcctcgtct cctccggctc tagctttgag ctgctcacag agctggacgt
     1321 ccacactctc gacgaccttc accagcgcgg tctggttgac gtggttgttt tccggcatct
     1381 ttcccatcct ctgctcgctt tcagtagcct agggatcgga gcacgttctc tctctgatcg
     1441 atccccggcg ctcgtgacag tggatgcctt accgaccgat ctcttcgtgg gcacactcca
     1501 cctacactta taaggatcgg cagaatcgcc gaaaacttgt gccccagttc gcggcccttg
     1561 ggctgatctg aatcggcgtc ggtgcccgac gtcacccctc gaggcagaaa tggttcacag
     1621 tggaactggg agttggggga aaagcgcgat tgggcgagtc gtctccgaga tgcacgcatc
     1681 gcgtcgctgg tactgcggga cttgtagctg tccgagatgg gggacggcag cacgagaatt
     1741 ggtggcccgg agaatgcaca ctgtttcagt tgtcacagta gtgtgataca atctgactct
     1801 actcgggaaa ccatcagcga gggtttccac ggaggtacca atgccggact cttccgcact
     1861 ctcatcgtct gccaggcgca aacgtgaggt catgtttgtt ccccaagcgg agcaaggccc
     1921 agtctgtcac tttgctgtcg atgatgcagc gctaattcgg ttcctgcggg acaattgcga
     1981 ggcgctggcc cccgtcatca gcagcatcac tgagttgcgg cgcccagtca acagaagcct
     2041 cggtgaggct gtcgcaagac tgttggtggt cactgacgag ccgattcaag ctgccttcgt
     2101 ttcgcgggca agcaatgcca tcgccaggat ggtttccgtt ctttcgccca gtaccctggg
     2161 agaggccgta ggggaaagct cggattatga ggtgcttcta gacgcgcttg aggcgcagga
     2221 agttgtgact gcacttcagg ccgacgaccc gctggcagca gcacgactgc gaggcataca
     2281 ggctcgcaat gaactcttga ctgctgaagg cggggtgctg ggtgtaggtg aagttgccga
     2341 gttgctgggc atcacccgtc aggcagtgga caagcgccgt cgaaacggca cactgttggg
     2401 actggcgaaa ggacagagca agtatgctta ccccgcgtgg caattcgctg gtcatagagt
     2461 gctcccaggc ttcgaagaag tcctggcttg tttcaaggtg gaagacccct ggactcgtgc
     2521 cggtttcttc ctaagtggaa atgctttgct gaatggacag cgaccgttgg atgtgcttcg
     2581 acagggccac atagaccagg tctgccgtgc cgcgggggct ttcggtgagc acgcctcctg
     2641 gtaaccccgg ggctggtgct caagccggcc cgggaacgag tgcgcggagt cctcacccac
     2701 tcccaccagc cacactcgcc gcagaggtac tccccataat cgaggtgacg atcagatggt
     2761 atcgtctggc agcaaacccg cgcacccccg tttactgggg gctaacacca ccacgcattc
     2821 cctcagcccg ctttgatgct cccaagggag agtttggcgt gctgtatgcc ggagtcgatg
     2881 cctactgcgc gtttatcgaa acctatggcc atgttacagg cactcgctgg gtcaccctag
     2941 ccgccctacg tgaaaggagc ctttttgaga tccgtttcgg ccatccgctg cggttggtaa
     3001 acctcacagg ccatcacctt gcggggctgg gggcggattc gggcctgttt tctggagact
     3061 acacagtctc gcaggcgtgg tcgctcgctt tcttcaacca ccccagtgcg ccggacggcc
     3121 tctactatca cacatgccac gacccgagcc ggatgtctct tgctctctac cagcggggcg
     3181 acattgagtc ccaggtatcc ttcacctccc tgggcactct cgcagattcc ggcaaccgtg
     3241 cgttgctggg aaagatactc gacacctatc gcttcgcctt gcttccctga cttcaacacg
     3301 agggcaaata ctctccgtaa tcccgcctgt gttcgtcaac ccaattgttg acggcttgtc
     3361 tgttacgtgc gttgtcctgg atgagtttct gaaccaactt ccagcgccgt agcctttgaa
     3421 cttagactcc acatgcactt acacagcaac gtcccatctg aatctcttct cggccgttcg
     3481 ttgttcacat cggtcattgc acttcttctt catacatgtt aagctgccga tactcggaag
     3541 aatgttgagt tgtgccctgc cggcgggcga gagccggcgc tccgggaggc accgtggctc
     3601 g
//
LOCUS       DATALS010000225         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_417412, whole genome shotgun sequence.
ACCESSION   DATALS010000225 DATALS010000000
VERSION     DATALS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417412"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..899)
                     /locus_tag="VHS06_04955"
     CDS             complement(213..899)
                     /locus_tag="VHS06_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX2987502.1"
                     /translation="MNTERTTATIVGVLYIVGTVAGFLSMVVTGGLLDAPDYLNVVAA
                     NASRTMLGALLVLVMGLALAMVPAMMFPILKRQNEALAIGYVVFRGALETVTYIASVI
                     GSLLLVPVSQAYLQAGVSDASSFQALGTLLLKARETSTTMTEIVFPLGALMFYYVLYQ
                     ARLVPRWISGWGIVAGIAYLSAGLIAVFSTNLVPLLLPMALQEMVMAVWLIARGFSRS
                     AIPSLSVQTN"
     gene            complement(1053..1760)
                     /locus_tag="VHS06_04960"
     CDS             complement(1053..1760)
                     /locus_tag="VHS06_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX2987503.1"
                     /translation="MKVCIVGASGKLGRYMVQHALDRGYEVVGVCRERSVSKLEAFKG
                     RIEVLPGATNDREVIEKAVAGCDGVLTVLFPWGAKYSSGTAQAVLDYARPGARLIFSC
                     GWHITRDGQDVYSPAFKATEKAMTWLTRLVPFVSIDDQVEACRRIFASDTLWTVVRGS
                     DLEEGDSEGLPAWSRHVGDAILESNLTRRVDFALFMVEALTDDRLIHETPAIVGCRTP
                     SALAHAAHAGRGVPRSA"
     gene            2249..2344
                     /locus_tag="VHS06_04965"
     tRNA            2249..2344
                     /locus_tag="VHS06_04965"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2283..2285,aa:Sec,seq:tca)
BASE COUNT          493 a          810 c          751 g          486 t
ORIGIN      
        1 aagcgctccc agcacgacta ggttcttgtt tgcagcaatc tgaacaaggt agtccggggc
       61 acccacgatg gatcccgtga ggccactacc cagtagggat gcagcggtcg cgatgatgaa
      121 caagactccc gcgatgatcg ctgttcttct gttcgtattc atttcttctc tccctgtcgt
      181 gactgagttt ggcgatgtcc tgttcactcg tttcagttcg tttgcaccga cagcgaaggg
      241 attgcggatc ggctaaaccc cctggcgatg agccagaccg ccatgaccat ctcctgcagg
      301 gccatcggga gcaacagggg caccaggttc gtgctgaata cggcgatcag gcccgcggac
      361 aggtacgcga tacctgcgac gatcccccag ccggagatcc agcgcggaac gagtctcgcc
      421 tgatacagca cgtagtagaa catcagcgca cccaggggaa agactatctc ggtcatcgta
      481 gtgctcgtct ccctcgcctt cagaagtagc gtgcccaggg cttgaaacga ggaggcatcc
      541 gagactccag cctgaaggta tgcctgactt accggcacca gtaggagtga gccgataacc
      601 gaggcgatgt aggtgaccgt ctccagcgcc cctcggaaga cgacgtaccc gatggccagt
      661 gcctcgttct gcctcttgag aatcgggaac atcatcgccg gaaccatggc aagcgcgaga
      721 cccatgacca gcactaggag ggctcccagc attgtccggc tcgcgttcgc ggccacgacg
      781 ttgaggtagt ccggagcgtc cagaaggccc cccgtgacaa ccatgctcag aaagccagca
      841 acggttccga cgatgtacag gacccccacg attgttgcgg ttgttcgttc tgtgttcacc
      901 tgctacctcc cctggtaggc gactcgtaca tgccaaagcg gctgagtgga acgcgacgcg
      961 gattcagaag cctttcggcc cttgccgtct cgttggccga gctcatctcg atcagtctcc
     1021 cccggattcg ccacaagcca gccgcatgct gttcaggctg atctcgggac tccacgtcct
     1081 gcgtgagcag cgtgtgctag cgcagagggc gtgcggcatc cgacaattgc cggggtttcg
     1141 tggatcaacc ggtcgtccgt aagagcctcc accatgaaga gggcgaagtc cacgcggcgc
     1201 gtcagattgc tctcgaggat ggcatcaccc acgtgccggc tccacgcggg cagaccctcg
     1261 ctgtccccct cttcgaggtc gcttccccgc acgacagtcc aaagggtgtc actggcgaat
     1321 atccgccggc aggcttccac ctgatcgtcg atgctcacaa aaggcaccag cctcgtgagc
     1381 caggtcatgg ccttctccgt cgccttaaag gcgggggagt acacgtcctg gccgtcccgc
     1441 gtgatatgcc agccacagga gaatatgagg cgcgccccgg ggcgcgcata gtcgagcacg
     1501 gcctgggccg tccccgacga gtatttagct ccccagggga acaggacggt gagcaccccg
     1561 tcgcagccgg cgacagcctt ctcgatgacc tcgcggtcgt tagtagcgcc gggaagcact
     1621 tcaatgcgcc ctttgaacgc ttcgagcttg cttacgctgc gctctcgaca gacaccaacc
     1681 acctcgtagc cgcggtcaag cgcgtgctgc accatgtacc tgccaagctt cccggaggcc
     1741 ccgacaatgc aaactttcat ctggcatcac cttgcacctg ttgtggccga ttacagtggc
     1801 agcgtatgcc ggaggatgac atgtggcatc aaccagaaga gggagtgtgg ctacgcgatt
     1861 cgctgactct tctcgttcag cattggagcg gacacgatcg gaaccccccc gacacccacg
     1921 acgcgatcga tccatggggg cactgcctgt ggcagaagcg tcttcgcgtc cacgctgtcc
     1981 tcacggacgg gctgaaagta gccataggcc tccggccgcc catctggaac gctcaacctg
     2041 gccggtgcaa tgacggggag gggggccggc cagggtcaat agcatgcccc cgacattcac
     2101 tgctgttccc cacgagtcgc tacgacctct acgggctcac cggaactcag acggaagagg
     2161 cggaacggct gcgctaggga ctaggatcgg ttacaatagt tgctagtggc cgttcgtcgt
     2221 gaggggcgat cagccacgag cacctcatgg aggcggacgg gtcccggtgg gcctcgcggt
     2281 cttcaaaacc gtctgcgggg cagtaatcgc tgtcccgggt gggttcgact cccatgcgcc
     2341 tccgccgtat acacaggccg ggcttgcttg cgtttagcag tgtttgcagg tccggcctga
     2401 tgattcgccg ccccttccac cgtcacggaa cgatgtggaa atgctgcagg cattctcccg
     2461 gtccaccacc tggatactcg gcccgtccga aagtcccctt atcttggaga tcgtcattgc
     2521 cgcgtctcct gcaggcgaag
//
LOCUS       DATALS010000226         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_24002,
            whole genome shotgun sequence.
ACCESSION   DATALS010000226 DATALS010000000
VERSION     DATALS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24002"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VHS06_04970"
     CDS             complement(<1..1010)
                     /locus_tag="VHS06_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987504.1"
                     /translation="MRRSSEFAEQLRTVYRPPIWLVIVGVVALVVGLAAWIVSIVIGP
                     GIDNWLWLVVWFLVFLGVSQGMLVWSATLRVAQARWAPAANRVGHSSIGMLPVLCAIL
                     IVLLVGLKGYMPWTVVPVPGKEFYLNLPFMVVRDLIGLGILFILDFLMVRWTLAADAR
                     ARRGEDVLRSEQFRLTAVGVAVIYTYVATASIISVDFIMAFSPEWVSTMFPPYYWCTN
                     VYAAMATVIVIAGLGRRLLGAGPYLGWGPFQDMGNLMLGFGFFCMGLFFAQYLTIWYE
                     NLPRETGFLVVRYYKGLWPALAWPAFALEFAIPFLLLQSRRLKRTPRLLVPVAILALI
                     GF"
     gene            complement(994..1569)
                     /locus_tag="VHS06_04975"
     CDS             complement(994..1569)
                     /locus_tag="VHS06_04975"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HEX2987505.1"
                     /translation="MAENFVRARYTEFLAFERALRTLKDAGVPDYEAFGPTNIEDLAD
                     LMPRREEPGGLLGRTKTLLGFGRTPADSPVRFWATLGGITGLLSFWLMCVLSSLLYVM
                     ITGGKPPVSNVPFVIPAYEGTILLGSLGAFAAAFYYARMWFGRRVSAHYDPHFSGDSF
                     GIVIRCEPRECRDMIQLLANSGAVEINEAEF"
     gene            complement(1572..2873)
                     /gene="nrfD"
                     /locus_tag="VHS06_04980"
     CDS             complement(1572..2873)
                     /gene="nrfD"
                     /locus_tag="VHS06_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX2987506.1"
                     /translation="MKLREIDEQVLGSLSPPKLGYMLALGLCMLLIGIGVVAYIVQYN
                     VGMGVTGLSIPVAWAVYITNFVWWVGIAHSGTLISAILYLFRTRWRNGINRAAEAMTI
                     FAIATAGLFPLIHLGRVWVFYWILPYPNWRHLWPNFKSPLVWDVVAVGTYFTVSLLFF
                     YLGMVPDLATARENSTGWRHSMYRILSLGWTGRYEQWRHYGRAYLALAALATPLVISV
                     HSVVSWDFAMSVLPGWHTTIFAPYFVAGAIHSGLAMVIMLMVPLRRILGLERVLTPYY
                     FEQMALLLILTGSIVGYAYVVEPFMAWYSADTYERYSITWRAVGTYAPFFWIMSIFNV
                     LTPATFFLKRLRTNLTYLFWASFLIQIAMWLERFVIIVTSLSRDFLPSSWRLYSPRPV
                     EILVTLGSLGFFLFLFLAFVKLFPSIPMSELKEQNLPHGED"
     gene            complement(2903..>4232)
                     /locus_tag="VHS06_04985"
     CDS             complement(2903..>4232)
                     /locus_tag="VHS06_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2987507.1"
                     /translation="AGAATAPGATLAPTPAPTATPAGAPPGPPPPLTPPGKRLRLWAY
                     PELYLYDGRGANRRWLQELPEPVIRGAWSSWAEIHSDTAKRMGVDPDDIIEITRGGSR
                     IQVPVYIWDGVQRDTVAVPIGEGHTAYGRYASGCGVNVWPVLGPGSPEITVKATGDRK
                     RVTRWKGDALQHKRDIVQTMPLGGSPGEREVTMPVPEGYGKLDFYPGHEHHLNRWAMA
                     IDVDRCIGCNACAAACYAENSLATVGEMGIYRAREMAWMRVDRYIDWSERSAPIHFLP
                     MLCQHCDAAPCEPVCPVYAAVHSEDGLNEQIYNRCIGTRYCSNNCPWKVRRFNWFDYE
                     WPEPLNYQLNPDVSVRSRGVMEKCTFCVQRIRQARIVAKREGRPIRDGEITPACVQTC
                     PTGVFTFGDLLDRNSRVARMIHGDPRAYQVLHELNTKTAVIYLKRIVERA"
BASE COUNT          946 a         1373 c         1188 g          725 t
ORIGIN      
        1 atgaacccta tgagagccag tatcgcgaca ggcacgagca gccggggggt tcgcttcaac
       61 ctgcggctct gaagcagcag gaagggaatg gcgaactcga gggcgaacgc cggccacgcc
      121 agcgccggcc acaacccttt gtagtatctc accaccagga accccgtctc cctcggcaga
      181 ttctcatacc agatggtcag atactgagcg aagaaaagcc ccatacagaa gaacccgaac
      241 ccgagcatca ggttgcccat gtcctggaac gggccccagc cgaggtacgg acccgccccc
      301 agcagccgcc gcccaagccc ggcgatcact atcaccgtag ccatcgcggc atagacgttg
      361 gtacaccagt agtaaggcgg gaacatggtg ctcacccact ccggggagaa cgccattatg
      421 aaatcgacgg agatgatgga tgctgtggcc acgtaagtgt atatgaccgc gactccaaca
      481 gccgtcagcc ggaactgctc cgaccgcagc acgtcctccc cccgtctcgc tctcgcatcc
      541 gccgcaaggg tccagcggac cataaggaag tcgaggatga acaggatgcc gaggccaatc
      601 agatctcgga ccaccataaa tgggagattc aggtagaact ccttcccggg tacgggcact
      661 acggtccacg gcatataccc cttcagcccg accagcagga ctatcagtat cgcgcaaagt
      721 accggcagca tcccaatgga ggagtgtccc acccgattcg ccgcaggcgc ccagcgtgcc
      781 tgggcgaccc tcagtgtcgc ggaccacaca agcatcccct gcgaaacccc gaggaacacc
      841 aggaaccata ccaccagcca cagccagtta tcgataccgg gaccgatgac tatcgagaca
      901 atccacgcag ccaaccctac gaccagcgca acaacgccca cgattacaag ccagatcggc
      961 ggccggtaga cagtcctcag ttgttccgca aactcagaac tccgcctcat ttatctccac
     1021 tgctccacta ttagccaata gctgaatcat gtctctgcac tccctcggct cgcatcgaat
     1081 cacgatccca aagctgtctc cgctgaagtg cggatcgtag tgcgccgaca cccgacgtcc
     1141 aaaccacatg cgcgcgtagt agaaggccgc cgcaaacgcc cccagcgacc ccagcagaat
     1201 ggttccctcg taggctggta tgacgaacgg gacgtttgat acaggtggct tgcccccggt
     1261 gatcatcaca tacagcagcg acgacaggac gcacataagc cagaacgaca acaggccggt
     1321 gatacctcca agagtcgccc agaacctcac cgggctgtcc gctggcgtgc ggccgaatcc
     1381 tagaagcgtc ttcgtccgtc ccaacagccc acccggctcc tcacgtctgg gcatcaagtc
     1441 cgccagatcc tcgatgttgg tcgggccgaa cgcctcatag tcgggcactc ccgcatcttt
     1501 caacgtgcgc agcgcacgct caaacgcaag gaactccgtg tagcgcgctc tgacaaagtt
     1561 ctctgccatc gctagtcctc accgtgcggc aggttctgtt ccttcagctc actcatcggg
     1621 atggacggga acagcttgac gaaagcgagg aacaggaaca ggaagaaccc cagcgaaccc
     1681 agtgtcacca gaatctcaac aggccttggc gaatagagcc gccaggatga aggaagaaaa
     1741 tcgcgcgaga ggctggtaac gatgatcacg aaccgctcga gccacatcgc tatctgaatc
     1801 aggaaactgg cccagaacag gtacgtgaga ttggtcctca gcctcttgag gaagaaggta
     1861 gcaggcgtga ggacattgaa tatgctcatt atccagaaga acggggcgta cgttccgact
     1921 gcacgccacg ttatcgagta gcgttcgtat gtgtcggcgc tgtaccaagc catgaagggc
     1981 tccacgacgt aagcatagcc cacgattgac cccgtcagaa tcagcagcag cgccatctgc
     2041 tcaaaataat agggcgtcag cacccgctcc aacccgagaa tcctgcgcag aggcaccatc
     2101 agcattatga ccatagcgag ccctgaatgg atggcgccgg ccacgaagta gggagcgaaa
     2161 atggttgtgt gccacccagg aagaaccgac atggcgaagt cccaagacac cacgctgtgg
     2221 acggagatga ccagcggggt ggcaagcgcc gccagggcaa gatacgcccg cccgtagtgc
     2281 cgccactgtt cgtaccgccc tgtccaacct agcgataaaa tcctatacat actgtgtctc
     2341 cacccggtgg agttctctct ggcagtcgcg aggtccggga ccatccccag gtagaagaag
     2401 agcagactca ccgtgaagta ggtgccgacc gctacaacgt cccagaccag aggcgacttg
     2461 aagttgggcc acagatgcct ccagttcggg tacggcagga tccagtagaa gacccacaca
     2521 cgcccgaggt ggatcagcgg aaacagaccg gccgtagcga tggcgaatat cgtcatagcc
     2581 tcagccgcac gattgatgcc gtttcgccat ctcgtgcgga acaggtagag gatcgccgat
     2641 atcagcgtgc ccgagtgcgc gattcctacc caccagacga agttggtgat gtagaccgcc
     2701 catgccaccg gtatactgag gcccgtaacg cccataccga cgttgtactg gacgatgtac
     2761 gcaaccactc cgattccgat gaggagcatg cagagcccca gcgccagcat atagccgagc
     2821 ttcggcgggc tgagcgagcc aaggacttgc tcgtcgatct ccctaagttt catcgcctct
     2881 tgagtcaaaa tgcccctccg tatcaggccc tctcaacgat gcgtttcaga tagatgactg
     2941 cagtcttggt gttcagctcg tgaagcacct ggtaggccct cgggtcgccg tgaatcatcc
     3001 tggcaaccct ggagttgcga tccaaaagat cgccgaaggt aaagaccccg gtcggacagg
     3061 tctggacaca ggccggcgtt atctcaccgt ctctgatcgg ccggccctca cgcttggcga
     3121 ctatcctcgc ctggcggatt cgctggacgc agaaggtgca tttctccata acgccgcgac
     3181 tcctcaccga gacatcgggg ttcagttggt agttcagggg ttcaggccac tcgtagtcga
     3241 accagttgaa gcgccgcacc ttccacgggc agttgttcga gcagtagcgt gtgcctatgc
     3301 agcggttgta gatctgctcg ttcagcccat cctcgctatg gacagccgcg tagaccggac
     3361 acaccggctc acacggtgca gcatcacagt gctggcagag catcggcagg aagtgaatgg
     3421 gagcggagcg ttcgctccag tcgatgtagc ggtcgacacg catccaggcc atctcacggg
     3481 cacggtagat gcccatctcc cctaccgtag ccaggctgtt ctctgcgtag cacgccgcgg
     3541 cgcaggcgtt gcagcctata cagcgatcca catctatcgc catagcccaa cggttgaggt
     3601 ggtgttcgtg ccccgggtag aagtccagct tcccgtagcc ttcgggcacg ggcatcgtta
     3661 cctcccgctc acccggcgac ccgccaagcg gcattgtctg aacgatgtcg cgtttgtgct
     3721 gcagcgcgtc ccccttccag cgcgtaaccc gcttgcggtc acccgtagcc ttgaccgtga
     3781 tctccggact ccccggaccg agaacaggcc acacgttgac gccgcagccc gacgcatagc
     3841 ggccgtaggc ggtgtgtccc tcaccgatgg gaaccgccac cgtgtcacgc tggaccccgt
     3901 cccagatgta gacaggaacc tggatcctgg aaccgcccct ggtgatctcg atgatgtcgt
     3961 ccgggtcgac ccccatccgc tttgccgtat cgctgtgtat ctcggcccac gacgaccacg
     4021 ctccccggat cacaggctcc ggaagctcct gcagccacct ccgattggcc ccccgtccgt
     4081 cgtagagata cagctccggg taagcccaca gccgcagccg cttgccgggc ggcgtcaggg
     4141 gcggtggtgg gcccgggggc gctccggcgg gtgtggcagt aggtgcaggt gtcggcgcaa
     4201 gtgtagcgcc tggcgcagtc gcagccccgg cg
//
LOCUS       DATALS010000227         9498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_157897, whole genome shotgun sequence.
ACCESSION   DATALS010000227 DATALS010000000
VERSION     DATALS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157897"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VHS06_04990"
     CDS             <1..1059
                     /locus_tag="VHS06_04990"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX2987508.1"
                     /translation="MQPEMEERARAIHRSAFVYDATLFDPGFLMDEKGEIQALTDGGL
                     SGANLTFCGHMHSFSKGVDSFHQHLKVIERNADRVRFCTSVEELEGCKKEGKVGIVAH
                     FQDGKPIEDSLDYLRLFHKMGLRILQLTYNVQNFIGAGCCERNDTGLSYFGLRVVEEC
                     NRLGILIDLSHCNHATAWDAVRHSKRPVAFTHVGIHQLCPAHGRNKPDDLLKAVVDTG
                     GVVGIVWFPPFVKRDPTTHQVLQATVDDVLDQMEYAINLLGADHVGIGTDLSDYNART
                     LEVPPDSSIRWYRTMRPDVFGAGPTDRYDPFPVGLDSHALMMNITRGLVKRGYSEDDI
                     RKILGGNWLRLQREIWGE"
     gene            1098..1478
                     /locus_tag="VHS06_04995"
     CDS             1098..1478
                     /locus_tag="VHS06_04995"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2987509.1"
                     /translation="MKKEIVAIPGVKPVKYLSRAVKFGNLVFVAGTTGRNLLTGEMPE
                     DAAGQTRVLLDNMKMVLESAGASMDSILKMTCYLANLADKPAFDEVYTSTFTADPPAR
                     ACFAVADLGAGVKVEIEAIAGIPQ"
     gene            complement(1566..2900)
                     /locus_tag="VHS06_05000"
     CDS             complement(1566..2900)
                     /locus_tag="VHS06_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2987510.1"
                     /translation="MQEQQPWVQKDLEYVLHSMHDHAHNVNGPLVLTKGQGSIVTDAS
                     GKEYIDCSAGLWCVNVGHGREDLAEAGAQQMRTLAYASGYAGTSNLPGIQLAEKLASL
                     APGDLKATFFTTGGAESTETAIKIARFYWKANGKSEKIKIISRLKGYHGTTAAAMVAT
                     GMSQYWKSFEPLQAGFVHIPTHYCFRCSLKQKYPECGVACADLLEQAILAEDPDTVAA
                     FIAEPIHGGGGVVVPPPEYFPRVREICDKYNVLFIADEVITGFGRTGKWFGMQQWDVV
                     PDILDFAKGVTSGYQPLGGVMVGEKIHKVFRELPEGASFNHAYTYSLHPTCCAVGVKN
                     LEIIEKEGLVERAAELGKRMQAGLKTLEDLPGVGEVRGMGLIAGIDLAADKSGTPMPP
                     AFGKKLVANAMADGLLLRSRGDVVWVSPPLVITESEMDRMIDGIAKAIKGAA"
     gene            complement(3018..5636)
                     /locus_tag="VHS06_05005"
     CDS             complement(3018..5636)
                     /locus_tag="VHS06_05005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2987511.1"
                     /translation="MDNRDMPVEGSGVRRRAARVVRFLAAGLALVLLLTLALSYLNVI
                     DWEQLGSEAGPPWDSVLNPALAPKALMLMAGEGLLFLSLWGLSRLLALRRIRLLLGEG
                     TVTVFRRLVQFGAWLPVVTAAYLLFRISPDTLRVDLDVHGDVVSLTLPTLAATLAFGL
                     AVMVTPPSPRAAYLPAPLSWAMALPFIPTALGAVLFLDQALGPSPAHGNVPLQLLVTQ
                     LSLLGVEILYLLMAAEAFSADWSDFRQANGWVQGGFALGRAWARLALFLAPLFLGLWL
                     ALGATLPAASVLRDGNGQFVQYYYPGSDFRLYTPSEAISPWVKTAIDAIEDPGVYTTP
                     FQHMPLKPVRIVGVFTGAARSVQDREGSYLSGASGIAAQSCKNYFGRRIVGMVWDLPD
                     WVPFRQLLAMAATVVHKLAFEFQCGWAYERSTLLLGAERSPVTFYLNAVYFGHGAYGV
                     EAASLTYFGKRAAELTLPEAALLAGLPQAPSVLDPWEHPDEVRARRTEVLQAMEREGY
                     ISQSDMLALDRSPLGVLAKPLEPNANAQRLGHYALFLADWLRSHGYGDPATAGLDVTI
                     SLDLERQKALDEQVRSAVNGLKGRNVNNGGAVVLDPRNGAVLAWFGGMQPIYGAEALP
                     DMAAAYPHQPGSALKPLLFACALQGGQLAPDERLDDTPREIGGRYIANWDGDPKGKGM
                     LSGAAALADSRNTIAASLVERMGTDGFVQCLRDRFQVRTDLAPEQHGVELGLGLAEMP
                     VLEVAAAFTVLANGGVYAEPTPSLMVRDRHGRELFRLQPAQGSRVLDPATLQWLREPL
                     SDISRRFGLPAGLYTKTGTTPSSSFAVGYGSDLVVAGWLGRTAPGRGPLDIDDVDGRE
                     AAERIWKPQALHQARP"
     gene            5891..7990
                     /locus_tag="VHS06_05010"
     CDS             5891..7990
                     /locus_tag="VHS06_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2987512.1"
                     /translation="MIRRLGSSSRILALSIIGLVLLLIGPVAVEAEQSQAKNAAAGSL
                     IAIEAPVPPIRAENPGQPTLPNKKRPKLDSRLGSVADELVKSGESPAVQAAQQHNLDV
                     IDNMVLVEVETRNQTRDSVRSSARQLGGQVEGEYANLLRILLPVSALERMAAHPDVLF
                     VGPPVRPVAESVAGQGVVASNTNLWHTAGIKGSGVKVAVVDLGFAGYTDRQASGDLPS
                     SLTIVDFTGGQFATGEPHGTAVAEIIHEMAPEAELYLIAISDRIGLGQAKDYAKSKGI
                     HIVNHSVAWFNTARGDGGGGPGTPDAIVADARSSGILWVNSAGNYAQRHWSGYFRDLD
                     EDNWHDFTREDDGNSFYLPSGSTVRIWLKWDSWPTTTQDFDMWLGRSSDNAMVANSWN
                     PQNGTEPPTEYIQYTNSGPSELFYVGVGKYGAITNPRLDLFISAADLEYQTPYGSVPE
                     PASSSNTMAVGAVYWQDNSLESFSSQGPTIDNRIKPDIAGQDRVYSASYDTGFAGTSA
                     SAPHAAGAAALVKQASPGFGPSQLQSYLEGRAIDRGTMGKDNLYGAGSLWLGAVPVQS
                     TPTSAPTSTPTAPEAATSTPTTTEPTPTATPVSTSEATAEPTNTATVAPVLTNTATAM
                     ATYTPTPTATAMPTEPPLPNLLQNPSFADHLTTGPCHPGCSQRCRSIQPRPMTATTMG
                     WCSRRQVAPSSHPRMSP"
     gene            7891..8919
                     /locus_tag="VHS06_05015"
     CDS             7891..8919
                     /locus_tag="VHS06_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987513.1"
                     /translation="MQIDPAAAYDGDDHGLVLSPSGSSFVASQDVAITPGGDFSLSGH
                     VQANQIDRGGFKVEMVFLNRYNGAVGSAVSKGYYQSTGAGIWDNFYIKGVAPTAAAKV
                     RVQLKSESLIGNVGVDALTLVQGEPSGPVATPTAGASPTPTAAAATPTPTTAAATPTT
                     TATATPTLANPTATPSATVSATATATPVPSATGNLLGNPSFRSPLAEDWIISSWAMSS
                     VRIDAAAAYDGDNSGLLQSSEGTTYNVTQDVTVTPGQKYRLSGFLKLPDLRSGSYRTE
                     LLFLNRYGGTVGSPVSLSYSRPTLDWQSFSQVVTVPAGAVKVRVQLKSNSMRGVAYVD
                     ALELAQTD"
     gene            9285..>9498
                     /locus_tag="VHS06_05020"
     CDS             9285..>9498
                     /locus_tag="VHS06_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987514.1"
                     /translation="MRIHPPLFPAIILGTLLLLASLPSSTLAAADWELPSGHHFTQAG
                     DYTVVDDAQAGFWTEFQRQGGVARIGY"
BASE COUNT         1918 a         3130 c         2859 g         1591 t
ORIGIN      
        1 atgcaaccag aaatggagga gcgggcgagg gcaatccacc ggtcggcgtt cgtctacgat
       61 gcaaccctct tcgatcccgg ctttctcatg gacgaaaagg gtgagatcca ggcccttacg
      121 gatgggggtc tcagcggagc caacctgacc ttctgcggcc acatgcacag cttctccaag
      181 ggggtggata gcttccatca gcacctgaag gtgatagagc ggaatgccga tagggtgcgc
      241 ttctgcacca gtgtcgagga actggaagga tgcaagaagg aagggaaagt gggcatcgtg
      301 gcccacttcc aggatggcaa gccgatagag gacagcctgg attacctgcg actcttccac
      361 aagatggggc tccggatcct gcagcttacc tataacgttc agaacttcat cggcgctggc
      421 tgctgtgagc gaaacgacac cggtctaagc tactttggcc ttcgggtggt ggaggaatgc
      481 aaccgactgg gcattctgat cgacctctct cactgcaacc acgccacggc ttgggacgcc
      541 gtccggcact ccaagcgacc agtggccttt actcacgtag gcatccacca actctgtcct
      601 gcgcacggcc gcaacaagcc cgacgatctg ctcaaggcgg tagtggacac agggggggta
      661 gtggggatcg tgtggttccc ccccttcgtc aagcgggacc ccacaaccca ccaggtgctt
      721 caggccacgg tggacgatgt gctcgatcag atggagtatg ccatcaacct gcttggcgcc
      781 gaccacgttg gcattggcac cgacctgtct gactataacg ccaggaccct ggaggttcca
      841 cccgattcca gcattcgctg gtaccgcacg atgcgacccg atgtcttcgg cgcaggtcca
      901 accgatcgat atgatccctt cccagtaggt cttgacagcc acgccttgat gatgaacatt
      961 actcgtggcc tggtgaagcg tggctactcg gaagacgata tccgcaagat cctcggaggt
     1021 aactggctcc gtttgcagag agaaatctgg ggcgagtagc caggaagctc gaccccgaaa
     1081 ctatgtagga ggacaccgtg aagaaggaaa tcgtggcgat cccaggcgtg aaaccggtca
     1141 agtacctgtc gcgggcggtc aagttcggaa acctggtctt cgtggctgga accacagggc
     1201 gcaacctgtt gactggcgag atgccggagg acgcagccgg ccagacacga gtgctgctgg
     1261 acaacatgaa gatggtccta gagagtgccg gggcatccat ggatagcatc ctgaagatga
     1321 cctgctacct cgccaacctg gccgacaagc cggccttcga cgaggtgtac accagcacct
     1381 tcacggcgga ccctccggca agagcctgct tcgctgtggc ggatctgggc gccggtgtga
     1441 aggtggagat cgaggcgatc gcgggcattc cccagtagaa acaccgcaac tggagagaca
     1501 cgaggaggag gcggaggacc atacgaatcc cccgcctcct ctccatgatt aggcttcgtt
     1561 ctcagttagg ccgcgccctt gatggccttg gctatgccgt cgatcatccg atccatctcc
     1621 gactcggtga tgaccagggg cggggagacc cacaccacgt caccgcggct ccgcagtaac
     1681 agtccgtccg ccatggcatt ggcaaccagc ttcttgccga atgcgggcgg catcggcgtg
     1741 ccggacttgt cggccgccag gtcgatccct gctatcagcc ccatgcccct gacctcaccc
     1801 acgccaggca ggtcctccag cgtcttcagg ccggcctgca tccgcttgcc cagctcggct
     1861 gcccgctcca ccaggccctc cttctcgatg atctcgaggt tcttcacacc caccgcgcag
     1921 caggtggggt ggagggaata ggtgtaggcg tggttaaagc tcgcaccctc cggcagttca
     1981 cggaagacct tatggatctt ctcgcccacc atcacgccac caagcggctg gtatccgctc
     2041 gtcacaccct tggcgaagtc gaggatgtca ggaaccacgt cccactgctg catgccgaac
     2101 cacttgccgg tgcggccaaa accggtgatc acctcgtcgg cgatgaacag cacgttgtac
     2161 ttgtcacaga tctctcgcac cctggggaag tactccggag gaggaacgac cacaccgcct
     2221 ccgccgtgga tcggctcggc gatgaaggcg gccacggtat ccggatcctc cgcgaggatc
     2281 gcctgctcca acaggtcggc gcaggccacg ccgcactcag ggtacttctg cttgaggctg
     2341 catcggaagc agtaatgggt ggggatgtgc acgaagccgg cctgcaacgg ctcgaagctc
     2401 ttccagtact ggctcatgcc ggtcgcgacc atggccgcgg ccgtcgtccc atggtagccc
     2461 ttgaggcgag agatgatctt gatcttctcg ctcttgccgt tggccttcca gtagaagcgg
     2521 gcgatcttga tcgccgtctc ggtggactct gcgccgccgg tggtgaagaa ggtggccttc
     2581 aggtcaccgg gggcgaggct ggccagcttc tcggccagct ggatgcccgg caggttggag
     2641 gtcccggcat agccggaggc atacgccagg gttcgcatct gctgcgcgcc cgcctcggcc
     2701 agatcctctc ggccgtggcc gacgttgacg caccagagtc cggcggagca atcgatgtac
     2761 tccttgccgg aggcgtccgt aacgatggat ccctgcccct tcgtcagtac caggggcccg
     2821 ttgacgttat gcgcgtggtc gtgcatcgag tgaaggacgt actcgaggtc cttctgcacc
     2881 cagggctgct gctcttgcat tccgcaactc ctcaagatga tactacctgg gagaggcgag
     2941 gctgaccgag aagaccagag caccgttggt caagtcctcc cagggaattc tttactgtcc
     3001 gagcatagca caaagggtca aggtcttgcc tggtgcagcg cctggggctt ccagatgcgc
     3061 tccgcggcct cccgaccgtc cacatcgtcg atatccaacg gtccccggcc gggcgccgtc
     3121 ctgcccagcc aacctgcaac cactagatca gacccatagc ccacagcgaa gctggaggag
     3181 ggcgtcgtgc ccgtcttggt atagagccct gctggaaggc cgaaccggcg tgagatgtcc
     3241 gagagcggct cccgcagcca ttgaagggtg gcgggatcca ggaccctgct gccctgagct
     3301 ggctgcaatc ggaacaactc tctgccatgg cggtctcgga ccattagaga gggcgtcggc
     3361 tcggcataca ctcccccatt ggccagcacc gtgaaagcag ccgccacctc cagtacgggc
     3421 atctcggcca gcccgagccc caactccaca ccgtgctgtt ctggggcgag gtccgttcgt
     3481 acctggaagc ggtccctcag acactgcacg aagccatctg tccccatccg ctccaccagg
     3541 gaggccgcga tggtgttgcg cgagtcggca agcgctgcag cacccgatag catccctttc
     3601 cccttgggat ccccatccca gttggcgatg tatcgccccc cgatctccct gggcgtgtca
     3661 tccagccgct cgtctggagc caactgccca ccctgcaggg cacaggcgaa caatagcggc
     3721 ttgagggcgc tgcccggctg atgtgggtag gcggcggcca tgtccggcaa cgcttcggcg
     3781 ccgtagatcg gctgcatccc cccaaaccag gcaagcacag cgccatttcg tggatccaga
     3841 acaacggctc cgccgttgtt cacgttgcgc cccttgagtc cattgactgc ggagcgcacc
     3901 tgttcatcca acgccttctg tctctcgaga tccagtgaga tggtcacgtc gaggccagcg
     3961 gttgcaggat ccccatagcc atgggagcga agccagtccg ccagaaagag ggcgtagtgg
     4021 cccagcctct gggcgttcgc gttgggctcg agcggcttgg cgagcactcc caggggtgat
     4081 cgatccaggg ccagcatgtc cgactgggag atgtaaccct cgcgctccat cgcctggagc
     4141 acctcggtgc ggcgcgccct cacctcatcg gggtgctccc agggatcgag aacgctgggc
     4201 gcctggggaa ggccggcgag caatgcagcc tcgggtagtg tcagctccgc cgcccgcttc
     4261 ccgaagtagg tgagggacgc agcttcgacg ccgtaggccc cgtggccgaa gtagaccgcg
     4321 ttcaggtaga aggtcacggg cgagcgttcg gcccccagca gaagggtaga gcgctcgtat
     4381 gcccagccac actggaactc gaaggccagc ttgtgcacca ctgtggcagc catggctagg
     4441 agttggcgga atggtaccca atccggcagg tcccaaacca tgcccactat ccgccgcccg
     4501 aagtagttct tgcacgactg tgcggctatc ccactggcgc cggagaggta gcttccctcg
     4561 cggtcctgca cggaccgggc cgcacctgtg aagacaccga caatcctcac cggcttgagg
     4621 ggcatgtgct ggaagggagt cgtgtatacc cctggatcct cgatggcgtc tatcgccgtc
     4681 ttcacccagg gcgagatcgc ctccgatggc gtgtagaggc ggaagtcgct gcccgggtag
     4741 tagtactgaa cgaattggcc gttgccatct cttagaacgc ttgctgcggg cagcgtagcg
     4801 cccagggcaa gccatagccc caggaagagc ggcgccagga agagggcgag ccgagcccat
     4861 gctcgcccga gcgcgaaacc gccctgcacc catccgttgg cctgtcggaa gtccgaccaa
     4921 tcggcgctga acgcctctgc ggccatcagc aggtagagga tttccacacc caacaggctc
     4981 aattgggtga cgagcagctg caggggcacg ttgccgtgcg caggcgaggg gccgagcgcc
     5041 tggtccagga agaggaccgc gcccagggct gtggggatga atggcagcgc catcgcccag
     5101 ctaagcggcg cggggagata ggcagccctg gggctgggag gggtcaccat gaccgccagg
     5161 ccgaaggcga gggtagccgc cagggtcggc agcgtgagcg aaaccacatc gccatgcacg
     5221 tccagatcca cgcgcagggt gtctggagat atgcggaaga gcagataggc ggccgttacc
     5281 acaggcagcc aggcgccgaa ctggaccagg cggcggaaca cggtgactgt gccctcaccg
     5341 agaagtaacc gaattctgcg gagcgcgagc agcctcgaaa gcccccagag cgaaaggaac
     5401 agcagcccct cgcccgccat cagcatgagc gccttgggag ccagggcagg gttcagtacc
     5461 gagtcccacg gcgggcccgc ctcgctgcca agctgctccc agtcgatcac attcaggtag
     5521 gacagcgcga gggtgagcag cagcaccaga gccaggccag cagcgaggaa ccggacgacc
     5581 ctggcagccc ggcgccgaac accactgccc tccaccggca tatctcggtt atccactacc
     5641 ggcgagcgac ctgtctcatc ggacgacaaa cgtcacctcg agcacccatg gaagtcgcct
     5701 gcagagcagt cgcacgagcc gtacccgcgc cgaacattca tccacttcta acctcgggct
     5761 gacacgacag tcatgaaggc gactctacat ccatgcaagt attcaccaca agtccttcgg
     5821 tcggcgctcg gaatggcctt ccgacatagc tgtcagcagg caatgccacc tgcggaggga
     5881 gattagggag atgataagac ggcttggctc gtcctcgcgc atcctcgccc tgtccataat
     5941 tgggctagtc ctgctgttga ttgggccggt ggcggtcgag gccgaacaga gtcaggcaaa
     6001 gaacgccgcc gcaggctcgc tcatcgccat cgaggcgccg gtacctccaa ttcgggcgga
     6061 gaacccgggt cagccaaccc ttcccaacaa gaagcggccg aagctggact ctcggcttgg
     6121 ctcggtcgcc gatgaactgg tgaagagcgg agagagcccg gcggtccaag ccgcgcagca
     6181 gcacaacctt gatgtgatcg acaacatggt cctggtcgag gtggaaacca gaaatcagac
     6241 ccgtgacagt gtgcgctcga gtgcaaggca gctcggcggg caggtagagg gagagtacgc
     6301 caatctcctc aggatcctgt taccggtttc tgcactggag cggatggccg ctcacccaga
     6361 cgtgctgttc gtcgggcctc cagtgcgccc ggtggccgag tcggtggcag ggcaaggggt
     6421 ggttgccagc aacaccaacc tctggcacac agccggaatc aaggggtcag gcgtaaaggt
     6481 agcggtggtc gacctgggct tcgcgggcta caccgaccgc caggcgagcg gcgatctgcc
     6541 atcctccctc accatcgtcg acttcacggg cggccagttc gctacaggcg aaccccacgg
     6601 cacagcggta gccgagatca tccacgagat ggcgcccgag gcggaactgt atctcatcgc
     6661 catatccgat aggatagggt tgggacaggc aaaggactat gcgaagagca agggcataca
     6721 tatagtcaac cattcggttg cgtggttcaa cacggccaga ggcgacggcg gaggagggcc
     6781 aggaacgccc gacgctatcg tggccgacgc gcgctccagc ggcatcctat gggtgaactc
     6841 agcgggtaac tatgcgcaga ggcactggag tggctacttc cgcgacctcg acgaggacaa
     6901 ctggcacgac ttcacgcgcg aagacgatgg gaacagcttc tatctgccaa gcggatccac
     6961 cgtccgcatc tggctaaaat gggacagctg gcccaccacg actcaggact tcgacatgtg
     7021 gctcggtagg tcgagcgaca atgccatggt ggcgaactcc tggaatccgc agaatgggac
     7081 cgagccgccc accgagtaca tccagtacac gaacagcggg ccatctgagc tgttctacgt
     7141 gggggtgggc aaatacggag cgatcaccaa ccccaggttg gacctcttca tctctgccgc
     7201 cgatctcgag taccagaccc catatggcag cgtccccgag ccagcctcat catcgaacac
     7261 catggcagtg ggcgcagtct actggcagga caacagcctc gagtcgttca gctcccaggg
     7321 gcccacgata gacaaccgca tcaaacccga tatcgcaggg caggataggg tgtactcggc
     7381 gtcctacgac acgggtttcg cgggcacctc cgccagcgcc ccgcatgcag ccggagcagc
     7441 cgcgctggtc aagcaggcaa gtcccggctt cggtccatcc caactgcaga gctacctgga
     7501 aggcagggcc atcgatcgcg gcacgatggg caaggacaac ctgtacggcg ccgggtcgct
     7561 ctggttgggc gcggtgcccg tccagagcac gcccacttcg gcgcccacca gcaccccaac
     7621 cgcgccggaa gctgccacca gcaccccaac cacaacggag ccaaccccga cagcaactcc
     7681 tgtcagcaca tcggaagcga ccgcagagcc aaccaacacg gcaacggtcg cacccgtctt
     7741 gaccaacacc gcgaccgcaa tggccaccta cactcccact ccaactgcga cggcgatgcc
     7801 gaccgaacca ccgcttccaa acctgctcca gaacccgtcc ttcgccgatc acctgacaac
     7861 tggaccatgc catcctggtt gctctcaaag gtgcagatcg atccagccgc ggcctatgac
     7921 ggcgacgacc atgggttggt gctctcgccg tcaggtagct ccttcgtcgc atcccaggat
     7981 gtcgccataa cacccggtgg agacttctcg ctgagcgggc acgtccaggc gaaccagatc
     8041 gacaggggcg gcttcaaggt ggagatggtc ttcctcaacc gatacaacgg cgcggtcggg
     8101 tcagccgtat ccaagggcta ctaccaatcg accggcgccg gaatctggga taacttctac
     8161 atcaaggggg tcgcccctac cgccgccgcc aaggtcaggg tgcagctgaa gagcgaatcg
     8221 ctgataggaa acgtcggagt cgatgccctg acactggttc agggggaacc cagcggcccc
     8281 gtcgccacgc cgacagctgg cgcatcgcca acgcccaccg cagccgcagc taccccaaca
     8341 cccaccacgg ctgcagccac cccaacgacg accgccaccg caacccctac cctcgccaac
     8401 ccgacggcca caccatcggc cactgtatca gccaccgcca cagctacccc cgtcccatcc
     8461 gccaccggga acctactggg caatccttcc ttccgctccc cgttggcaga ggactggatc
     8521 atctcctcat gggccatgtc cagtgtacgg atcgatgcag cggctgccta tgatggcgat
     8581 aatagtgggc tgctccagag cagcgagggc acaacctaca acgtgaccca ggacgtcacc
     8641 gttacaccag gtcagaagta ccggctcagc ggctttctca agctgccaga tcttcgttcc
     8701 ggctcctaca ggaccgagct gctgttcctg aaccgctacg gcggcaccgt cggatctccg
     8761 gtctcgctta gctactcacg gcccaccctc gattggcaga gtttcagcca ggtggtcacc
     8821 gtcccggccg gtgcagtgaa ggtccgagta cagctgaaga gcaacagcat gcgcggcgtc
     8881 gcctacgtcg acgccctgga gttggcacaa accgactgat ccatcctaga actccgccaa
     8941 actggtctca attgatcgcc ttcagcgatc caatgctggg agcgcgggcg gcccgcccgc
     9001 ccgttccccc aggcccccca tcccatttgt ccgatcggtc cgatccgact gatccgtccg
     9061 atccacccac tgcccccgca agccctcgcg gcgagccgcc cgcgctccca gacgtcacga
     9121 agtagcccct attggtgatt gcagcatcca caaccttgag ttagagtagt atctctcgat
     9181 catgtaacta catgcccggg cgcactcccg gctcatccgg aacaaggcac gcgctgaccc
     9241 accgcacctc agccaactac ctctcatcag gaggctacac tgcgatgcgc atccacccgc
     9301 cgctgttccc tgcaatcatc ctcggcacac tactcctgct cgcctcgctg ccatccagca
     9361 ccctcgctgc ggccgattgg gaactgccca gcgggcacca cttcactcag gcgggagact
     9421 acaccgtcgt cgacgatgcg caggcgggct tctggaccga gttccagcgg caggggggag
     9481 ttgcgagaat aggctatc
//
LOCUS       DATALS010000228         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_380844, whole genome shotgun sequence.
ACCESSION   DATALS010000228 DATALS010000000
VERSION     DATALS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380844"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VHS06_05025"
     CDS             <1..301
                     /locus_tag="VHS06_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2987515.1"
                     /translation="TAVDLSLEEVVERSQGSGGLCVPAHVDRPANGLLSVLGMLPEDL
                     RAPALEISPRIDIDGALKQYPQLAGNTLIRSSDAHTLEDIGKVATETASRSGQTG"
     gene            complement(536..886)
                     /locus_tag="VHS06_05030"
     CDS             complement(536..886)
                     /locus_tag="VHS06_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HEX2987516.1"
                     /translation="MMITVGDVAERLSAKVVAGEERLARPVTGGYVGDLLSCVMAGAS
                     AGDVWVTVQGHPNVVAVATLVGISAILVAEGARIDPGTVEKANQEGIPLLSSQKRSFV
                     LVEGLVGIGVRASD"
     gene            complement(886..>1687)
                     /locus_tag="VHS06_05035"
     CDS             complement(886..>1687)
                     /locus_tag="VHS06_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2987517.1"
                     /translation="RFHRISGLPVASNGSIDGILSIEDVIRWLESGGQEAPASDWMTR
                     KVLTVRGDEAAVQAISKFGRHKVGRLPVVDRAGALIGIVTSGDIIGRALRVLDARYRE
                     EEARRGAARCTLDELVGDGTSFVLRYVVAPRDFQHAGYAATKVKRILDGLGMDRKVVR
                     RVGIAAYEAEMNLSMHTERGGSLRAEIGPETIVIEAQDDGPGIADIEQALTAGYSSAP
                     DWIRELGFGAGMGLTNMRNASDRFDIDSELGRGTTVRVEINLIAGKGA"
BASE COUNT          295 a          592 c          474 g          326 t
ORIGIN      
        1 gacggcagtc gacctttcgc tggaggaggt ggtggagcga tcccaagggt ccggtgggct
       61 gtgtgtgccg gctcatgttg accggcccgc caacgggctt ctttccgtgc tgggtatgct
      121 tccggaggat ctccgggcgc ccgcgctgga gatatcgcct cgtatcgaca tagatggggc
      181 gttgaagcag tatccacaac ttgccggcaa cacactgatc aggtcgtcgg atgctcatac
      241 gctggaagac atcggcaagg ttgccactga aacagcttct cgatccggtc aaacagggtg
      301 aggacgtgga ccccctccct ggtctcgacc tcgatgccgg gaaggactct caccccgacg
      361 tccgcccctg cagctatcgc ggcctccgcg ttctccgcgg agttgtggtc cacgactccc
      421 agcaagctta tgcctcggtc cctggcccgg cgaacgatca ggtccgggac cattcggtag
      481 tcggcgcatg gcgacagcac cgtatgaacg tgcaggtccg ccacgaaacg gtccatcagt
      541 ccgaagccct tacccctatc cccaccaggc cttccaccag cacaaaggag cgcttctgac
      601 tcgagaggag cggaattcct tcctggtttg ccttctccac cgtaccaggg tctatccgcg
      661 ccccctccgc gaccagaatt gcggagatcc ccaccagtgt cgcgacggcc accacgttgg
      721 ggtgtccctg caccgtgacc cacacgtcgc ccgcggatgc ccctgccatc acgcaactga
      781 gcaggtcccc cacgtacccg cccgtcaccg ggcgagcgag gcgctcctcg ccggccacga
      841 cctttgccga cagtctttcc gccacgtcgc cgactgtgat catcattatg cccccttccc
      901 ggcgatcagg ttgatctcaa cccgaactgt ggtgcctcta cccaactccg agtcgatgtc
      961 gaagcgatcg gaggcattcc tcatgttggt cagccccatc cccgcaccga agcccagctc
     1021 tcggatccag tcaggagcag acgagtagcc ggccgtgagg gcctgctcga tgtcagcgat
     1081 cccagggccg tcatcctggg cttcgatgac tatggtctcc ggcccgatct cagccctaag
     1141 ggatcctcct ctttctgtgt gcatcgacag gttcatctcc gcctcgtagg cggcgatgcc
     1201 cacccttctt acgaccttgc ggtccattcc cagtccatcc aggattcgct tcaccttggt
     1261 ggcggcgtag ccggcgtgct gaaaatccct cggggccacc acgtagcgga gtacgaaaga
     1321 ggtgccatct cccaccagtt cgtccagggt gcagcgtgcg gcgcccctcc gtgcttcctc
     1381 ctcgcgatat cgggcgtcca gcacccggag tgctcttcca atgatgtcgc ccgacgtcac
     1441 gatgccgatt agtgcacccg ctcggtcgac cacaggaagc ctgccaacct tgtgccggcc
     1501 gaacttggaa atcgcctgga cggccgcctc gtcgccgcgg accgtcaaca ccttcctggt
     1561 catccagtca gacgcaggag cctcctggcc gccactctca agccaccgta tgacatcctc
     1621 gatgctgagg atcccgtcga tggagccgtt cgaggcaaca gggagtcctg agatcctgtg
     1681 aaagcgc
//
LOCUS       DATALS010000229         7855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_281189, whole genome shotgun sequence.
ACCESSION   DATALS010000229 DATALS010000000
VERSION     DATALS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7855
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281189"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VHS06_05040"
     CDS             complement(<1..552)
                     /locus_tag="VHS06_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2987518.1"
                     /translation="MSTVTLTIDGRQVQAEAGSSILEAAKQAGIHIPTLCYHPDLTVR
                     AVCRICVVEVKKSRTLQAACAYPVADGLEVTTNTPRVRAARKANLELLLSNHPFECPT
                     CVRNQNCELRALANEYNITQVRFEGERKQYLVDSSSYSLVRDPNKCILCRRCVDVCNS
                     LQEVGAIGPVHRGFDTVIGPAYDE"
     gene            complement(586..2382)
                     /gene="nuoF"
                     /locus_tag="VHS06_05045"
     CDS             complement(586..2382)
                     /gene="nuoF"
                     /locus_tag="VHS06_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX2987519.1"
                     /translation="MGTCGIAAGAREVMHAILHEIEALNLDVTVVATGCVGLCSQEPL
                     VDIEVPGGLRVTYGKVTPQMVPTLISDHLVKGHVVTQWAVARGSTTPAPVRPQPETVK
                     AIPTYDDIPFYGKQVRIALRNCGVINPEEIQDYIVRGGYEALGRAILEMTPQDIIDTV
                     KKAGLRGRGGAGFPAGLKWEFARKAAGDVKYIICNADEGDPGAFMDRSILEGDPHSVV
                     EGMCIAARAIGAQEGYIYCRAEYPLAIKRLERAIQQAHEHGLLGENILGSDFSFDLRI
                     KQGAGAFVCGEETALIASIEGRRGEPRPRPPFPAVSGLWGKPTSINNVETWANVPPIL
                     LHGPEWFSSIGTEKSKGTKVFALTGKVNKPGLVEVPMGITMKEIIYDIGGGIPNGKKF
                     KAVQTGGPLGGCLPTSMLDTPVDYDSLTSAGATMGSGGMIVIDEDTCMVEFAKFFLTF
                     ATDESCGQCTPCRIGGKRMLEVLTRITEGKGTMDDLKEIEQLARTMADTSLCALGQLT
                     PNPTLSSLKHFRDEFLAHIVDKKCPAGVCTALLHFEISPDKCKGCSLCLINCPSGAID
                     GARKQVHKIDQSKCIKCGSCYDVCRLDAVIKS"
     gene            complement(2570..3049)
                     /gene="nuoE"
                     /locus_tag="VHS06_05050"
     CDS             complement(2570..3049)
                     /gene="nuoE"
                     /locus_tag="VHS06_05050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX2987520.1"
                     /translation="MQMTASPAIDLSQVDAILERRKAEKGAVIPILQETQEAYGYLPR
                     EVIDRIAKTMGVPFSQLYGVATFYSQFYLSRRGKNIIKVCDGTACHVRGAARTVDTIH
                     SDLGVEPGGTTEDYGFTFEVVYCLGACALAPVAVVNGQVKARCTAERMRQTIKDMTK"
     gene            complement(3325..4836)
                     /locus_tag="VHS06_05055"
     CDS             complement(3325..4836)
                     /locus_tag="VHS06_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987521.1"
                     /translation="MLAMGRQANILVVDDEKGIREGCRRVLLAEGAQVAVAADAEAGL
                     ELASGGSFDLALIDVKMPGMGGIELVRRLQQIDPTLVAVMITGYATLETAIEATKNGA
                     YDFLPKPFAPDELVAKVNKGLERRWLMLEARRLRQERERRLLEISAEKSRLLTIINCM
                     QDGVLVTNQDGQLVLHNPAARTMLQLRVEGSGPQWVDSCIPLPDISALVASVLSSEAP
                     EGMLSQEVPGPDGHRTLMANVAAVRDERAEIIGAVTVVRDITGLKRLEKAKSQFVGMV
                     AHELAAPLGAIKGYLDVLIDGATGDDPVQHRQMMERSRDRAAALLDLIDDLLEDSQLE
                     SGQVSRRFESVSIPDAVSDAWEVVRPQAERRGIEYLPELPTGLPPVLADRRDMARVFT
                     NLLSNAIKYNREGGTIRVDARPDCNYLEVNVTDTGLGIPSDALGRLFEQFYRVKSPET
                     RSITGTGLGLSIVKRIVEAHHGFVRVESQVGVGSRFTVYLPAVGDNRPVAGPE"
     gene            complement(4796..5242)
                     /locus_tag="VHS06_05060"
     CDS             complement(4796..5242)
                     /locus_tag="VHS06_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987522.1"
                     /translation="MAVKGKILLIDDDVDFVTMNRAILEHGGYDVLAAFGGQEGIELA
                     RREQPDVVVMDVMMDDTTEGFELSRQLRTDPLLSRLPIILLTSINRHFRPLSFKPDDN
                     WIPVERLLDKPVRPQQLLNEIETVLKSRRPPHGDAGYGATGEHPGR"
     gene            complement(5268..7292)
                     /locus_tag="VHS06_05065"
     CDS             complement(5268..7292)
                     /locus_tag="VHS06_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEX2987523.1"
                     /translation="MAAIVTTIRDRCKRCYSCIRSCPAKAIKVEDGQAKVMEERCITC
                     GNCYKVCAQGAKQVQSSVGAVREMLRWKEPVIAAIAPSFPASFDGITPGQVIGAVKML
                     GFDEVMEVAFGAELVSREYVRLADRQRDVPIITTPCPAAVTLIQKFYPALIPFLAPIV
                     SPAIALGRAIKTEYRPDARVVFVGPCIAKKAEIHENAVAGAIDEVLTFAELKELFRQA
                     GISIDGAVEGEVDGPRPRIGRIYPLQGGLLRTAAMKSDVLESDIVVIEGKDNVLRVLR
                     EMADGKLAVRFLDILFCEGCIDGPVIESDHGLFARRQLVSHYVQSHLGEQSTEQFERT
                     VARFSAVDLSRGFEETSVVPPSPSDGEIDAILRQTGKMSKADELNCGACGYGTCREKA
                     TAVYQGLAELEMCLPYLLDRFQLELQAAQDQLVQSEKLASMGQLAAGVAHEINNPLGT
                     IMIYASLLLKEYHHDDQRWSDLKLIVEEAERCRSIVSGLLEFARQGKLELRQTDLNVL
                     LQDVVAQVNRLPCFDGVTVSLQLDTTLPTIAADPAQLRGVFSNIVVNAAEAMPDGGRI
                     TISSGVESGARSVWITVADTGCGIAEENLRKLFVPFFTTKEIGKGTGLGLAIAYGIVK
                     MHRGTLDVQSKLGQGSSFTVTLPVEGPQGRSSAKAMARGQLIGDITETPS"
     gene            complement(7531..>7855)
                     /locus_tag="VHS06_05070"
     CDS             complement(7531..>7855)
                     /locus_tag="VHS06_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HEX2987524.1"
                     /translation="LPVKVAVAHGLSNAKVLMDKVRAGEANYHFIEVMCCPGGCIGGG
                     GQPVPTSPEIRRKRAEAIYREDRGLPLRKSHENPEVQELYKEYLEKPLGHKSHELLHT
                     HYHAR"
BASE COUNT         1405 a         2520 c         2306 g         1624 t
ORIGIN      
        1 ctcgtcgtag gcgggaccga tcaccgtgtc gaagccccga tggacgggtc cgatggcccc
       61 gacttcctgc agggagttgc agacgtcgac gcagcgcctg cacaggatgc acttgttcgg
      121 atcccggacc agggagtatg aactactgtc caccaggtac tgcttgcgct cgccctcaaa
      181 gcgcacctgg gtgatgttgt actcgttggc caacgcacgc agttcgcagt tctggttgcg
      241 cacgcaggtg gggcactcaa agggatggtt ggacagtaga agctccaggt tggcctttct
      301 ggcagcccgg acgcgtgggg tgttggtggt aacctccaac ccgtcggcta cgggataggc
      361 gcaggcggcc tggagcgtcc tcgacttctt gacctcgacc acgcagatgc ggcagaccgc
      421 acgcaccgtg agatcggggt ggtagcacag tgtagggata tggatgcctg cctgcttcgc
      481 agcctcgagg atactgctgc cagcctccgc ctggacctgc cggccgtcaa tagtcagtgt
      541 caccgtcgac atttatcgtg acctcctcca gtatgctatt cgtctctatg acttaatgac
      601 ggcgtcgagt cggcagacgt cgtaacagga accgcacttg atgcacttgc tctggtcgat
      661 cttgtgcacc tgctttcgag ccccatcgat ggcaccggaa gggcagttga tcagacagag
      721 agagcagccc ttgcacttgt cgggtgagat ctcgaagtgg agcaaagccg tgcagacccc
      781 ggcagggcac ttcttgtcga cgatgtgagc caggaactca tctcggaagt gcttgagtga
      841 gctgagggtc gggtttggcg tcaactggcc cagcgcgcag agagaggtat cggccatcgt
      901 ccttgccagc tgctcgatct ccttgagatc gtccattgtc cccttgccct cggtgatgcg
      961 agtcagaacc tccagcatcc gcttgccgcc aatccggcag ggggtgcact ggccgcagga
     1021 ctcgtccgtg gcgaaggtaa ggaagaactt ggcgaactcg accatgcagg tgtcttcgtc
     1081 gatgacgatc atgccgccgg agcccatggt cgcgccggca ctggtgaggg agtcataatc
     1141 gaccggggtg tccagcatgg aggtagggag gcacccaccc aacgggccac ccgtctggac
     1201 agccttgaac ttcttgccgt tggggatgcc gccaccgata tcgtagatga tctccttcat
     1261 ggtgatgccc atcgggacct cgaccagtcc aggcttgttc accttgccgg tcagggcaaa
     1321 caccttagtt cccttgctct tctcagtccc gatgcttgag aaccattcag gcccatggag
     1381 aagaatgggc gggacgttgg cccaggtttc gacgttgttg atactagtcg gcttgcccca
     1441 gagaccagaa acggccggga acggaggccg cgggcgtggc tcgcctcgcc gcccctcgat
     1501 ggaggcgatc agcgccgttt cctcgccgca tacaaacgcg ccggctcctt gcttgatccg
     1561 cagatcgaac gagaagtcgc tgcccaggat gttctcaccg agtaggccgt gctcgtgcgc
     1621 ctgctggatc gctctctcca gccgcttgat cgccagcggg tactcggcgc ggcagtaaat
     1681 gtagccctcc tgagccccga tggcccgcgc cgcgatgcac atgccctcta ccacggagtg
     1741 cggatcgccc tcgaggatgc tgcggtccat gaaagcgccc gggtcgccct cgtcggcgtt
     1801 gcagatgatg tacttgacat caccagcggc cttccgggcg aactcccact tgaggcccgc
     1861 agggaagcct gcgccaccgc gaccgcgcag gccggccttc ttgacggtgt cgatgatgtc
     1921 ctgaggtgtc atctccagga ttgctcttcc cagcgcttcg tagccgcccc ttactatgta
     1981 gtcctggatc tcctcggggt tgattacgcc gcagttcctc agggcaatcc tgacctgctt
     2041 tccatagaag ggaatgtcat cgtaggttgg gatcgccttg acagtttcgg gctgcgggcg
     2101 gaccggcgcc ggggtggtgg agccgcgagc cacggcccac tgggtgacca cgtgcccctt
     2161 gaccagatgg tccgatatga gtgtggggac catctgcggg gtgaccttgc cgtaggtgac
     2221 ccgcagcccg ccggggacct cgatatccac cagcggctcc tgggagcaga gacctacgca
     2281 accggtagcg actacggtga cgtccaggtt cagcgcttcg atctcgtgaa ggatggcgtg
     2341 catgacttct cgggccccgg cggcgatccc gcaggttccc atgcctatgg tgatcttcgt
     2401 ggcggactcg ccacgcgcgg agatttcgct aatggcctgc tcttttagct gcctcaactg
     2461 ctccagtgca ctcggttgcg gcatcgaact cctcctcaat tggcgttagc gggacgcatg
     2521 catctgcgat ggctcgaaag gccaacacac caggggcggg aagcccccgt cacttggtca
     2581 tgtcttttat ggtctggcgc atccgctccg cggtgcagcg agccttgacc tgtccgttga
     2641 cgacggcaac cggtgccagg gcacaggcac ccaggcagta caccacctcg aaggtgaaac
     2701 cgtagtcctc cgtggtccca cccggttcga cgcccaggtc gctgtggatc gtgtcgacgg
     2761 tgcgggcggc gccgcgaacg tggcatgccg tgccgtcgca gaccttgatg atgttcttcc
     2821 ctcgcctgct gagatagaac tgagagtaga aggtggctac accgtagagt tggctgaaag
     2881 ggactcccat ggtcttcgcg atccggtcga tgacctctct cgggagatag ccataagcct
     2941 cctgagtctc ctgcaggatg gggatcaccg ctcccttctc ggccttgcgc cgctccagga
     3001 tggcgtccac ttgcgacagg tcgatggcgg gggatgctgt catctgcatg gacttggcct
     3061 ccatctgatt tcgattgggc ttctgctgtt actccagcat acttcgtgcc agaaggcaca
     3121 aactacggca caataaacgg ctccagcggc atgtcccgaa acatcgagga acgcgctgga
     3181 gccctccatc agccccggcg tatgaacttc gccaccattg gtgaagtttt tcacaggcat
     3241 tatatacctg ggaaacagtc tgtcaatggt ttgtttgttg tcgtcaatgg tttgttgctt
     3301 gaaacgaatg gtgccacgcg cgagtcattc agggcctgcg acggggcggt tgtcccctac
     3361 tgccggtagg tacacggtga agcgactccc cactcccacc tggctctcca ctctcacgaa
     3421 gccgtggtgt gcctccacta tcctcttcac gatagacaaa cccagccctg tgccggtgat
     3481 actcctcgtc tcggggctct tgacacgata gaactgctcg aaaagccggc cgagggcgtc
     3541 cgaaggtatc cccagccctg tgtcggtcac gttcacttcc aggtagttgc agtccggcct
     3601 ggcatctact cgaatagtcc ccccttctct gttgtacttg atcgcattgc tgagtagatt
     3661 ggtgaagact cttgccatat ccctgcggtc ggccaggacg ggcggaagtc cagtcggaag
     3721 ttccggaagg tattcaatcc cccgacgttc ggcctgaggg cgcaccacct cccatgcatc
     3781 ggagacggcg tcgggaatag acacggattc gaagcggcgg ctcacctgtc ccgattccag
     3841 ctgcgagtcc tcaaggaggt cgtctatcag atcaagcagt gctgccgctc tgtcccgcga
     3901 cctttccatc atctgacggt gctgcaccgg gtcgtcaccc gtggctccgt ctatcagcac
     3961 gtcgaggtat cccttgatcg cgcccaacgg cgcagcgagc tcgtgggcaa ccatgccgac
     4021 gaactgggac ttcgccttct ccagccgctt caagccggtg atgtcccgca cgaccgtgac
     4081 tgccccgatg atctcggccc tctcgtccct cacggcagct acgttggcca tcagcgtccg
     4141 atggccgtct gggccgggca cttcctggga gagcataccc tccggtgctt cagaggacag
     4201 caccgaggcg acgagggcag agatgtctgg gagagggatg caactgtcca cccactgtgg
     4261 ccctgagcct tccacacgga gctggagcat agtgcgagcg gcggggttgt ggaggaccaa
     4321 ctgtccgtcc tggttggtca ccagaacgcc gtcctgcatg cagttgatga tggtgaggag
     4381 gcggctcttc tcggccgaga tctccagtag gcggcgctcc cgctcctggc gaagccgtcg
     4441 cgcctccaac atcagccagc gacgctccaa ccccttgttc accttcgcga ccaactcgtc
     4501 aggggcaaaa ggctttggca ggaagtcgta cgcgccgttc ttggtggcct ctatggcggt
     4561 ctcgagggtg gcgtagccgg tgatcatcac ggcaacgaga gtagggtcta tctgctgaag
     4621 gcgccgaacc agctcaatgc cacccattcc cggcatcttg acgtcgatga gggccaggtc
     4681 gaagctgccg ccactggcta gctcgagacc cgcctccgca tcagcggcga ctgcgacctg
     4741 agcaccctcg gccagcagaa cgcgacggca cccttcccgg atgccctttt cgtcgtcaac
     4801 gaccaggatg ttcgcctgtc gccccatagc cagcatctcc gtgtggcggt ctccggctct
     4861 tgaggacagt ctcgatctcg ttcagcaact gctgcggtcg gaccggcttg tcgagcagcc
     4921 gctccaccgg gatccagttg tcgtccggct tgaagctgag agggcggaaa tgtcggttga
     4981 tcgaagtcag aaggatgatt ggaagtcgtg aaagaagtgg gtccgttcgg agttgtctgg
     5041 acagctcgaa cccctcggtg gtgtcgtcca tcatgacgtc catcaccacc acgtcgggct
     5101 gctcccggcg ggcgagttcg atcccctcct gacctccgaa ggcggcgagg acatcgtagc
     5161 caccgtgctc gaggatcgcc cggttcatgg tcacgaaatc gacgtcgtca tcgatcagca
     5221 ggatcttgcc cttgactgcc atcacacacc gcctctcaag aaccggccta gctcggcgtt
     5281 tcggtgatgt cgccaatcaa ttgtcccctg gccatcgcct tcgcagacga tctaccctgg
     5341 ggaccctcaa cagggagggt gacggtgaag ctgctgccct gtcccagctt gctctggaca
     5401 tccagggttc cgcgatgcat cttgactata ccataggcaa tggccagccc caagcctgtt
     5461 cccttgccaa tttccttggt ggtgaagaaa ggcacgaaca gctttctgag attctcctca
     5521 gctatcccgc accccgtgtc cgcgacggta atccacaccg atcgcgcccc actctcgacg
     5581 ccactggaga tggtgatccg gcccccgtcc ggcatcgcct ctgcggcgtt gacgacgata
     5641 ttggagaaga cccctcttag ctgggccggg tcggcagcta tggtaggcag agtggtgtcc
     5701 agctgaagcg acactgtgac tccgtcgaag cagggaagcc tgttcacttg tgccacgacg
     5761 tcctgtagaa gaacgttcag gtcggtctgg cgcagctcca gcttcccctg gcgggcgaac
     5821 tctagaagcc cggatacgat ggagcggcag cgctccgcct cttcgacgat cagcttcagg
     5881 tccgaccagc gctgatcatc gtgatggtac tccttcaaca gcaggctggc atagatcatt
     5941 atcgtgccca gggggttgtt gatctcatga gcaacccccg ccgcgagctg gcccatcgac
     6001 gccaacttct cagactggac gagttggtcc tgcgccgctt gcagctcaag ctggaaccgg
     6061 tcgagcaggt acggaaggca catctccagt tcggctaacc cctggtacac ggcggtggcc
     6121 ttctctcggc aggtcccgta gccgcatgct ccacagttca gttcgtccgc cttgctcatc
     6181 ttcccggtct gtcgcaggat ggcgtcgatc tccccatcag aagggctggg cggaacgacg
     6241 ctggtctcct cgaagccgcg agagagatcg acggccgaaa agcgcgcgac cgttctttcg
     6301 aactgctcgg tgctctgctc gcccaggtgg ctctggacat aatgtgagac cagttgtctt
     6361 cgcgcgaaca gtccgtggtc gctttcgatc accgggccat cgatacagcc ttcgcagaag
     6421 aggatatcca gaaacctgac cgccagcttg ccgtccgcca tctcccgcag cacccggaga
     6481 acgttgtcct tgccctcgat gacgacgatg tccgactcga ggacgtcgct cttcattgcg
     6541 gccgttctca agaggccacc ctggagggga tagatgcgtc cgattcgtgg ccgcgggccg
     6601 tcgacttcac cctcgacggc tccatcaatc gatatccctg cctgacggaa cagttccttc
     6661 agttccgcga aggtgagtac ttcgtcgatg gcaccggcca ccgcgttttc atggatctca
     6721 gccttcttgg cgatgcaggg gccgacgaat accacccgag cgtctggtct gtactcggtc
     6781 ttgatcgccc tccccagggc aatcgcggga gagacgatcg gcgcgagaaa ggggatcagt
     6841 gccggataga acttctggat caacgtcacc gcggccgggc acggtgtcgt gattatcggc
     6901 acgtcgcgtt ggcgatcagc caaccggaca tactctctgc tcaccaactc ggcgccaaag
     6961 gccacctcca tcacctcgtc gaagccgagc atcttcaccg caccgatgac ctgcccaggg
     7021 gtgatgccgt cgaaggaggc agggaaggag ggagcaatgg cagcgattac aggttctttc
     7081 caccgcaaca tctccctcac cgcgcccacg ctgctctgta cctgcttggc tccctgagca
     7141 cacaccttgt agcaattgcc acaggtgatg cagcgctcct ccatcacctt cgcttgtccg
     7201 tcctccacct ttatcgcctt ggccggacag cttcggatgc aactgtagca gcgcttgcag
     7261 cgatctctga ttgtcgtgac tatggctgcc atatcacgtt cgccgttctc gcagaaaccc
     7321 acacgtccac gccgcgttcg ggccgcctat gccgtctcgt agggcggggt cttgtgcccc
     7381 gcttcccaca ctgtggctac gcccaccatg gcagtctccg gcaacgccct gggcggggca
     7441 caagaccccg ccctacgacg cgtttacggc gtgcctctcc tcattgcaga gccgcatcat
     7501 gccttgcggc ccgttcacgg agtcctccca ctacctggcg tgatagtggg tgtggagcag
     7561 ttcgtgggac ttgtgtccca acggcttctc caggtactcc ttgtacagct cctgcacctc
     7621 ggggttctcg tgcgacttcc tcaagggcag ccccctgtcc tctcgataga tcgcctccgc
     7681 cctcttcctc cggatctccg ggctcgtcgg caccggctgc cctcctccac ctatgcaccc
     7741 gccgggacag cacatcacct cgatgaagtg atagttcgcc tccccagcac gaaccttgtc
     7801 catcagcacc ttcgcattcg acagcccgtg cgccaccgcc accttcacag gtaga
//
LOCUS       DATALS010000230         7041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_281190, whole genome shotgun sequence.
ACCESSION   DATALS010000230 DATALS010000000
VERSION     DATALS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281190"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VHS06_05075"
     CDS             <1..325
                     /locus_tag="VHS06_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HEX2987525.1"
                     /translation="LPVKVAVAHGLSNAKVLMDKVRAGEANYHFIEVMCCPGGCIGGG
                     GQPVPTSPEIRRKRAEAIYREDRGLPLRKSHENPEVQELYKEFLEKPLGHKSHELLHT
                     HYHAR"
     gene            679..2268
                     /locus_tag="VHS06_05080"
     CDS             679..2268
                     /locus_tag="VHS06_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2987526.1"
                     /translation="MARKLKSLTALVLPLVMSAVLVACVPQTTPESAPQSAPKPNAVV
                     YGTTFQPNTLNPITAPDIVSRSAIEMIFSGLVAANDKGELRPALGESWEESADGKVWT
                     FKLRHGVKWHDGQEFTAEDVKFTYDTVIDPNTKPTVAKSDYAGLQRVEVVDPYTVRFY
                     LAQPNAAFLARLVLGIAPKHLLQGQDLATATFNRQPVGTGPFVLDSWVKGESLTFKRN
                     TDYFGEKPKVEKIVWKIVPDSSVLALQASNGEVDGAPVFNPKDAAALQSSGKLALHET
                     LEGNTQISLQERNLLFQDVRVRQALAYAIDTHALIDAVLAGAAVPATSDILPSSWAYN
                     PDVPTYEYDPSKAKALLADAGWRPGSDGILTKDGKRFSMSLMTDAGHKVREQVMLAVR
                     QYWGEVGIEVKASTQERNSFIFDRVLKGDFDAVLLQTAVQIDPDLSRRFHTKSIKNGQ
                     NFLNYSNPKLDTLLEQALATSDQQQRKQAYAAAQKILAEDVPQICLFHPKTQYAFKSQ
                     LQGIKPSPTNLFWNAEQWEWK"
     gene            2259..3224
                     /locus_tag="VHS06_05085"
     CDS             2259..3224
                     /locus_tag="VHS06_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2987527.1"
                     /translation="MEVSSAILSRTWQAVLLLFLVSLVTFVLVRLAPGDPSAFFFGSS
                     ISAEDAAQFRARWGLDDPVHIQYVRWAANALGGDLGRSYQDGRPVLTVIAERVPASLL
                     LALSALVMATVLGVSLGVVAASRYYSMWDRAITLVATLCYSTPAFWLGIVLILLFSVR
                     LGWLPSGGIRNPAAASDWTDLLRHLTLPAAALAVRDVGRFARVTRSSMLEVLSQDYLR
                     TATAKGLGPRTITLRHTLRNALLPVITLLGMAIPGLLSGSVIIETVFGWPGMGRLAIE
                     SALQRNYPVIMGEVLIVAAMAIAGSLLADITCSLADPRLGRTGRG"
     gene            3228..4139
                     /locus_tag="VHS06_05090"
     CDS             3228..4139
                     /locus_tag="VHS06_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2987528.1"
                     /translation="MFGLVSLHPRGKSASGIVTPHKGLSQATFIVRRGSRLCDPLTIG
                     AVALLIALAIGAMLAPFLSSYDPTRIDLRQAGLPPGPVHPMGTDQLGRDVWSRALYGG
                     RVSLAVGLAAAAIAALLGGVLGVTAAYHGGWVDAAVTRLVDAALAMPAFFLLIAAQSI
                     MGQGVINIIVMVSLVGWMMVARVVRTLTLSLKEREFVLAARALGCSGARIVIRHLVPN
                     LAGQVGVLFALGVADALLLEAALSFLGIGVPPTEPSWGNMLNDAQAAILSGAWWIAAF
                     PGLLVLGTALSINLVGDRIQEMLTPRL"
     gene            4157..5071
                     /locus_tag="VHS06_05095"
     CDS             4157..5071
                     /locus_tag="VHS06_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX2987529.1"
                     /translation="MRRKNPNWIAFALLLLAVTAAVVGAWFLVADSNRRSGATPGDTG
                     LLLAVLDVGQADSIFLRSPSGRTMLVDAGNEKSDTEKVILPFLARLGVSKLDYLVLTH
                     PDQDHVGGMPYLLDSVAVGAFVDSVQPKITNQAYSQTLQRVQGKGVTPIKARRGQAEL
                     DLGTGIQTQILEPEDPLFSKVDSLTNNNGVVLRITYGTVSTLLTGDLGKEGEERLLSH
                     KEDIQSQILKIGHHGSESSSTNAFLEAVNPEVGLISVGSGNSYGHPHRPTLQRLERHK
                     VTVYRTDQNGTIQLRIDGRGYTVSPGRS"
     gene            5071..5319
                     /locus_tag="VHS06_05100"
     CDS             5071..5319
                     /locus_tag="VHS06_05100"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HEX2987530.1"
                     /translation="MRKMENGVHLWVTYDRVEEDERGRKIAVLLFDDGQELHLPAGYL
                     PRDSAPQQVLEVTLRADLQETERRAEDVRRRQQQLFGE"
     gene            5324..6835
                     /locus_tag="VHS06_05105"
     CDS             5324..6835
                     /locus_tag="VHS06_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2987531.1"
                     /translation="MDRTLWKAVQGVLLALLLVVAGYAIQSPPQATETKSAGVATVTK
                     AGAVSPGSSGTTPVATLIRSAAAAPTPGAVSSALPGSVGLSTPSLGTVAVPPTAAVSA
                     VTPTISSVRVAPTGTPIAFSDAEALRQLRQLADEIGARPTDSVSQRQAAAMLKERFFT
                     LGYSPTMQPYDFFTFQSRSSRLLLQKPETAEVIGTTFYFSGGGQVEGRLVACGLGRPS
                     DFPRAGLAGQVALIARGGGISFESKVANAVSEGAAAAVIYNDRTGDFKGSLRQPAAIP
                     VIGILQYDGERLLEYLKAGPTLVGLTVDAQSVTHSGENVIARLPARGNGKKVIVGAHY
                     DSFSSVPGANDNGSGVATLLEVARAARGRAFPFDVEFVLFGDEEVGLVGSKKYVASLS
                     KEQRSQLIAMVNLDMVGAGTRLEAGGDHALVGRVQQAGLRDDYQIAELPPGQNSTSDH
                     ASFLEAGIPAVMLHRGEDPNYHTSLDTSDRVLPANLDAAGTITLRLLGDFAKR"
BASE COUNT         1340 a         2131 c         2211 g         1359 t
ORIGIN      
        1 tctacctgtg aaggtggcgg tggcgcacgg gctgtcgaat gcgaaggtgc tgatggacaa
       61 ggttcgtgct ggggaggcga actatcactt catcgaggtg atgtgctgtc ccggcgggtg
      121 cataggtgga ggagggcagc cagtgccgac gagcccggag atccggagga agagggcgga
      181 ggcgatctat cgagaggaca gggggctgcc cttgaggaag tcgcacgaga accccgaggt
      241 gcaggagttg tacaaggagt ttctggagaa gccgttggga cacaagtccc acgaactgct
      301 ccacacccac taccacgcca ggtagctaga gagacgtagg gcgggcatcg ggccgccgtg
      361 aggtagtagg cgggattgat cgggacgtag ggcggggtct tgtgccccgc ccagagcgtc
      421 gccggagact gccatggtgg tcgtgaccgc cgtgtgggag gcggggcaca ggaccccgcc
      481 ctacgaccta ccataggtgg cctgcccagc atggacgtgt ggggtcgggt cgcaacgacc
      541 agtctagaga cggctacctt tcaagtctgg ggcggatcgg gaacggtccg ccctactcta
      601 ttcctgccgt gatacactat ccggaaccca gagatgacta caccggaccc tcgcaggccc
      661 tagagaggac tcttcaccat ggcaagaaag ctgaagtcac tcactgctct cgttctcccc
      721 ttggttatgt cggctgtcct tgtcgcttgc gtgccgcaga ccacgccgga gtcggcgcca
      781 caatcggcac ccaagccaaa cgcagtcgtg tatggcacaa ccttccagcc aaacacgctg
      841 aaccccatta ctgcacccga tatcgtctcc cggtcggcca tagagatgat cttctccggc
      901 ctcgtggccg ccaacgataa gggcgagctt cgccctgcac tgggcgagag ttgggaggaa
      961 tcggccgacg gcaaggtgtg gaccttcaag ttgaggcacg gggtgaagtg gcacgatgga
     1021 caggaattca ccgcagagga cgttaagttc acctacgata cggtcatcga cccgaacacc
     1081 aagccgacgg tggctaagtc ggactacgcc ggtctgcaga gggtggaggt cgtagaccct
     1141 tacaccgtcc gcttctacct cgcacagccg aacgccgcct ttctcgctcg tctggtgctg
     1201 ggcatagcgc cgaagcatct cctccagggg caggacctgg ccacggccac cttcaaccga
     1261 caaccggtcg gcaccgggcc ctttgtcctc gatagctggg tcaagggcga gagcctcacc
     1321 ttcaagcgta acacggacta cttcggcgag aagcccaagg tggagaagat cgtctggaag
     1381 atcgtgccgg acagcagtgt cctggccctg caggcgagca acggcgaggt ggacggcgcg
     1441 ccggtcttca acccgaagga tgctgcagcg ctgcagtcct cggggaaact cgcgttgcac
     1501 gagacgctgg agggcaacac acagatcagc ctgcaggaga ggaatttact cttccaggac
     1561 gtgcgggtcc ggcaggcgct ggcatatgca atcgacacgc atgcactaat cgatgccgtc
     1621 ctcgcagggg ccgcggttcc cgcgacttcc gacattctgc cctcatcgtg ggcctacaac
     1681 ccggacgtgc cgacttacga gtacgatccg agcaaggcaa aggcgctgtt ggccgatgcc
     1741 ggatggcggc cgggtagtga tgggattctt acgaaggacg gaaagcgctt ctccatgtcg
     1801 ctcatgaccg atgcgggcca caaggtaaga gagcaggtga tgctcgccgt ccgccagtat
     1861 tggggtgagg tgggaatcga ggtgaaggcg agcacccagg agaggaactc cttcattttc
     1921 gatagggtgc tgaagggcga tttcgacgca gtcctccttc agacagccgt gcagattgat
     1981 ccggacctct cacgaaggtt tcacaccaag tccataaaga atggacagaa cttcctgaac
     2041 tacagcaacc cgaagttgga tacgctgctg gaacaggcgc tagcaacttc cgatcagcaa
     2101 cagcgaaagc aggcgtacgc tgccgcgcag aagatactcg cggaggatgt gcctcagatc
     2161 tgcctcttcc acccaaagac gcaatacgct ttcaaatccc agttgcaggg gatcaagccg
     2221 tcgcccacga atctgttctg gaatgcagag cagtgggagt ggaagtgagc agtgcgatcc
     2281 tgagccgcac ctggcaagcc gtgctgttgc tctttctggt gagcctcgtg accttcgttc
     2341 tggtgcggct ggcgcccggc gatcccagtg cctttttttt cggtagctct atctcggctg
     2401 aggatgccgc ccagtttcgg gcgcgatggg gcctggacga ccctgtacat atccagtacg
     2461 tccgttgggc agcgaatgcg ctgggtggcg acctgggccg ttcctatcaa gacggtcggc
     2521 cagttctgac cgtgatcgcg gagcgagtgc cggcatcact gctgctggcg ctctccgcgt
     2581 tggtgatggc aacagtactt ggggtgagtc tgggcgtagt tgctgcctct cgctactact
     2641 cgatgtggga ccgtgccatc acgttggtgg ccacgctctg ctactcgacg cctgccttct
     2701 ggttggggat cgtgttgatc ctgctctttt ccgttaggct ggggtggctc ccgtccggag
     2761 gaatacggaa cccggcggca gcctcagatt ggaccgatct actgcgccac cttacgctgc
     2821 cggcggcggc tctcgccgtt cgagacgtgg ggcgctttgc ccgcgtcacc cgctcctcca
     2881 tgctggaggt gctatcccag gactacctgc ggaccgccac agccaaaggg ctcggtcccc
     2941 gcacaatcac cctgcgccac actttgcgca acgccctcct ccccgtcatc accctgttgg
     3001 gcatggccat acccggcctc ctcagcgggt cagtcatcat tgagacggtc ttcgggtggc
     3061 cgggcatggg gcgcctcgcg attgagtcgg cccttcagcg caactatccg gtgatcatgg
     3121 gagaggtcct aatcgtcgcc gctatggcca ttgcgggcag tctattggcg gacatcacat
     3181 gctctctggc cgacccccgg ctcgggcgaa ccgggagggg gtagctcatg ttcggtcttg
     3241 tgagcctgca ccctcgtggg aagtctgcct cggggatcgt cactccccac aaagggcttt
     3301 ctcaggcgac gttcatcgtc cgacgagggt cccgcctctg cgacccacta accatcggtg
     3361 cagtggctct ccttattgcc ctggccatcg gggcaatgtt ggccccattt ctttcgtcct
     3421 acgatcctac gcgcatcgac ttgaggcagg ctgggctccc acccggccct gttcacccca
     3481 tggggaccga ccaacttggt cgcgatgtat ggagccgcgc cctctacggg gggagggtct
     3541 ccctggccgt tgggcttgcc gccgccgcaa tcgccgcgct tcttggaggt gtcctcggtg
     3601 tgaccgccgc ctatcacggc ggctgggttg atgcggccgt cacgaggttg gtggatgccg
     3661 ccctcgcgat gccggccttc ttcctcctca tcgccgctca gtcaatcatg gggcagggcg
     3721 ttatcaacat catcgtaatg gtgtcgctgg tcggatggat gatggtagcc cgggtcgttc
     3781 gaacactcac gctcagcctg aaggagcggg agttcgttct ggctgcgcga gcgttggggt
     3841 gctccggtgc caggatcgtc attaggcacc tggtacccaa tctggctggg caggttggcg
     3901 tcctatttgc cctcggggtt gccgacgctc tgcttctgga agcggctctc agctttctag
     3961 ggatcggcgt gccgccgaca gagcccagtt ggggaaacat gctcaacgat gctcaggctg
     4021 ctatactgtc tggcgcctgg tggatcgccg cgttcccggg attgcttgtg ctgggaacgg
     4081 ccctctccat caatctggtg ggtgaccgca tccaggagat gctcactcca cgcctctgaa
     4141 gaggtccggc tgaagcatga gaagaaagaa ccccaattgg atagcctttg ccctgttgct
     4201 gcttgccgtc acggcggcgg tcgtgggtgc atggttcctc gtcgcagaca gcaataggcg
     4261 gagcggtgca acccccggcg acaccggcct ccttttggcc gtcctcgacg tcgggcaggc
     4321 cgatagcatc ttcctgcgat ctccctctgg ccgaactatg ctggtggacg caggaaacga
     4381 gaagtccgac accgagaagg tgatcctccc cttcttggcg cgactcggtg tctccaagct
     4441 cgactacctg gtgctgactc atccggacca ggaccacgtc ggcgggatgc cctaccttct
     4501 ggacagcgtt gccgttggag ccttcgtgga ttcggttcag ccgaagatta ccaatcaggc
     4561 ctacagccag acgcttcaac gggttcaagg gaagggggtc acaccaatca aggccagacg
     4621 cggccaggcc gaactggatc tgggcacggg gattcagacg cagatactgg agccggagga
     4681 tcctctcttc tcaaaggtgg attcgcttac caacaacaat ggagtcgtgc tgaggatcac
     4741 atacggcacg gtatccacgc tactgacggg agatctgggc aaggagggcg aggagaggct
     4801 tctctcccac aaggaggata ttcagagcca gatcctgaag atcggccacc atggtagcga
     4861 gtccagcagc accaacgcct tcctggaggc cgtgaacccc gaggttggcc tgatatcagt
     4921 gggaagcggc aactcgtatg gccatcccca ccgccccaca ctccaacggt tggagcgcca
     4981 caaggttacg gtctatcgga ccgaccagaa cggcacaatc cagcttcgga tcgacggccg
     5041 cgggtataca gtcagcccgg ggcgttcgtg atgagaaaga tggaaaacgg cgtacatctc
     5101 tgggtcacct acgacagggt ggaggaggac gagcgaggaa ggaagatagc cgtcctcctc
     5161 tttgacgacg ggcaggagtt gcatctccct gccggatact tgccacgaga ttcggctccg
     5221 cagcaggttc tggaggtcac ccttcgagcg gacctccagg aaaccgagag gcgagccgaa
     5281 gatgttcggc gccgccagca acaactcttc ggtgagtagc aacatggatc gaaccctttg
     5341 gaaggcagtt caaggggtct tgctggcgct gcttctggta gtggccggct acgccatcca
     5401 atccccacca caggccactg aaaccaaatc tgccggtgtg gctaccgtga cgaaggcggg
     5461 tgccgtgtcc cctggctcgt ccggcaccac acccgttgcc acactcattc gctcggcggc
     5521 tgccgctcca acaccgggcg ctgtctcctc tgctctgcct ggatcagtgg ggttatcgac
     5581 gccgagtctt gggacggtgg cggtgccccc cacagcagct gtttcggccg tcactcctac
     5641 catttcttcg gtgcgagtgg cgcccaccgg tacgccaatc gccttttcgg acgcagaggc
     5701 cctgcgacaa cttcgacagt tggcagacga gattggtgcg cgcccaaccg acagcgtatc
     5761 ccaaagacag gccgccgcca tgctcaagga gcgtttcttc acacttggct attcgcctac
     5821 aatgcaaccc tacgactttt tcactttcca gagtcgatcc tctcgccttc tcttgcagaa
     5881 gccggaaacc gccgaggtta tcggtacgac cttttacttc tcgggcggag ggcaggtgga
     5941 gggccggctg gtggcctgtg gattgggccg accgtccgat ttcccgcggg cagggcttgc
     6001 aggacaggtg gcgttaatcg cgcgaggcgg aggcatctca tttgagagca aggtcgccaa
     6061 cgccgtctcc gagggcgcgg ccgccgcggt gatctacaac gaccgaacgg gggacttcaa
     6121 gggaagcctg cgtcaacctg ctgcgattcc cgtgattggg atcctccagt atgatggcga
     6181 gcgacttctc gagtacctca aggctgggcc gacactggtt ggtctgaccg tggacgcgca
     6241 gtcggtcacc cactctgggg agaacgtgat cgccagattg cccgcaaggg gcaacgggaa
     6301 gaaggtcatc gtcggcgcac actacgattc cttttcgtcc gtgcctgggg caaacgataa
     6361 cgggtccggc gttgcgacac tgttagaggt tgccagggca gcgaggggac gcgctttccc
     6421 cttcgacgtc gagttcgtcc tcttcggaga cgaggaggtg gggttggtcg gcagcaagaa
     6481 gtatgtggcg tcgctctcca aggagcaacg atcgcaactg atagccatgg tgaacctaga
     6541 catggttggc gccggtacca ggctggaggc gggcggcgac catgccctgg tcggtcgcgt
     6601 tcagcaagcc ggcctccgcg acgactacca gatcgccgag ttgccgcccg gccagaacag
     6661 caccagcgac catgcgagct ttctggaggc cggcatacca gcggttatgc tgcaccgcgg
     6721 tgaggacccc aactatcaca cttcactcga cacctccgac agggtccttc cggctaacct
     6781 ggacgcggca ggaacgatca ccctcaggct gctgggcgat ttcgccaaga gatgactcac
     6841 cgctccagcg ctcgcctttt cccccgaagc ccctctgtac tacaatggct gtggccggca
     6901 ctgtagaggg ccgatcgttt tcgagcgccg aattcaccct ggacaagttg agggccagga
     6961 tggggggcgt gtagtccctc caaagagact ggagcaaggc aatgaaccaa ggaacccgaa
     7021 ccgaacgaga caccatgggg g
//
LOCUS       DATALS010000231         8786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_158159, whole genome shotgun sequence.
ACCESSION   DATALS010000231 DATALS010000000
VERSION     DATALS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8786
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158159"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VHS06_05110"
     CDS             complement(<1..766)
                     /locus_tag="VHS06_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987532.1"
                     /translation="MVVRLFGGGSKSQPAPDPDLDPRDPDPRQLVMAWDQEAREKAGS
                     ARAAKKTKAVPKTAASKDSKPSRGAKDIPPAKPASPTTKSTASAAKTTAGRVKASTGA
                     ATPTLFDEPKATLRNGAATGGRVATTVADPASEVSVEIPAQQRSAPAISTANSHAAVS
                     GQPEAVAGNGRNGNGNGNGSDDSASYTGDQIQILEGVQHIRQRPGMYIGTTSTSGLLH
                     LVYEAIDNVVDEFNAGYGTSMEVTIDSDGRVTVKDEA"
     gene            1309..1728
                     /locus_tag="VHS06_05115"
     CDS             1309..1728
                     /locus_tag="VHS06_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987533.1"
                     /translation="MTDGCTQYDDLHGSIDEVIEKLDSIRHWLRTYSESHEVSGRDSR
                     DTLGLMAGYAESLSQAMFKVQSSIGEERSVAADAIWTLLVVTGQVRNWLISNGRASAL
                     SRRDPDTQQLMLAYSVVAGQQADILKTILREYKDGQG"
     gene            complement(1759..2331)
                     /locus_tag="VHS06_05120"
     CDS             complement(1759..2331)
                     /locus_tag="VHS06_05120"
                     /inference="COORDINATES: protein motif:HMM:NF040096.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987534.1"
                     /translation="MNPNETVWTGNEASAKVASHTPGRLRVRLDRNRRSRESLHEARR
                     SLELREGIRQVEGNSTTGSLLLHYDPEQLNHDQLLSILKDAGVIVRDILEAGGTELPA
                     VGKSTTATSITDSLNDLDRRLSSITGRRLDLKLLFPATLGALGLRQVLTQGLGLNQVP
                     AYVLLWYAFDSFWKFHAGRSPEPVEAGAQQ"
     gene            2505..3035
                     /locus_tag="VHS06_05125"
     CDS             2505..3035
                     /locus_tag="VHS06_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX2987535.1"
                     /translation="MEGFFQLGAPAWQIVLRSVGIYLALLIGLRLFGKREVGQFTIFD
                     LAFVLLVANAVQPAMTGPDNSFVGGVLIIATLLVANWSVGWLRLHTPVFREAFESHPT
                     VIAEDGRWFTDVMDREGVNLQEAEMALREHGVENVQGARLVVLESDGGISVVPRESGG
                     KEGGHRRRVRYRKQQT"
     gene            complement(3087..4073)
                     /locus_tag="VHS06_05130"
     CDS             complement(3087..4073)
                     /locus_tag="VHS06_05130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX2987536.1"
                     /translation="MSKPKLFITRPVIDSTMARLSAQCEVSFWHEDSRPLQDEMERAI
                     ADAEACMGFAPWPAALMDKAPKLRIIANTAVGFDNVDVPAATQRGIVVTNTPNVLTDT
                     TADLAFTLMLTVSRRICEADRFVRGGKWTMQGGPATFLGSDVNHATLGIIGLGRIGAE
                     VAHRATGFHMTVLYYDPIRREELEKQFGYQYVDMDTLLAQSDFVSVHMPLMPQTKGLI
                     GAAQFAKMKPTAFLINAARGPVVDEKALIAALKEKRIAGAGLDVFEQEPTPTDNPLLK
                     MDNVVVAPHIGSATVATRQAMVDLAADNVLAVLAGKAPLTPVNPEVLSRLKK"
     gene            complement(4135..4800)
                     /locus_tag="VHS06_05135"
     CDS             complement(4135..4800)
                     /locus_tag="VHS06_05135"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HEX2987537.1"
                     /translation="MNEESRVSVTRGTEEDRRTTNRGRRLSDVLRALSVVADIGFQRS
                     SRGLSEMTGTPIEAKAAQVRKVPMPQVPQLVGGEEILTAAAYVKIVGDVEGHMLLLLP
                     MQDALRLVSMVLGEPEEAGAELSDMARSALGEVGNITCSFFLCALADSTGLEISPSPP
                     AVAVDMAGAVMDTVLADLATENDEVFVIDTHFAHSDNTVNAIFLVLPRLRYIDLILDR
                     LPK"
     gene            complement(5047..5526)
                     /locus_tag="VHS06_05140"
     CDS             complement(5047..5526)
                     /locus_tag="VHS06_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2987538.1"
                     /translation="MTLHIRPLTEKDIPTFLDLVQGLADYESLPGPDEEARARMAQDA
                     LADPPVFHVLLAEQDGRLVGYSLFFFTYSTFLARPTLWVEDIFVLPSERGNGNGRMLM
                     RELAREAVRRGCGRMEWSALDWNKTAIAFYEGLGAKQMSEWRLFRLTATELAIVAED"
     gene            5806..6438
                     /locus_tag="VHS06_05145"
     CDS             5806..6438
                     /locus_tag="VHS06_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX2987539.1"
                     /translation="MLPDLPTLALFMFATLMLNVTPGPDMLYVVANSVGRGQRAGVVS
                     AFGIGAGTLVHTLAAAFGLSALLMSSSLAFDVVRYGGAAYLIYLGIRSLTGRESPIPS
                     GRLEREGLGKIFRQGMATNVLNPKVALFFLAFLPQFVDPARGSVVVQFILLGTLFNTS
                     GTTVNSLVAVASGYAGERLGKSPGFARTQRWFSGGVFVALGARIALTGAR"
     gene            complement(6440..7420)
                     /locus_tag="VHS06_05150"
     CDS             complement(6440..7420)
                     /locus_tag="VHS06_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2987540.1"
                     /translation="MMERRNPVTTSTSIAWRIHGLLRLIWSPQAPCPIPRRRSTADDS
                     WWTPAKRNFLRTPALYLGVALIAMIAVGAVVVPLFCPYTPDQILPNSRLLPPNATHPF
                     GTDALGRDLLIRVAAGAWLAARIAVMSVGISLAIGLILGSLAGYYSGWTDQLISRAMD
                     GWLSLPGALVAIVIVARLGASLDNLILALGFMGVPAFYRIVRNTTLSARHMPYAEAAI
                     ALGADDRRVMWRHVFPNILSPVVVLTSMRMGTALLTGSGLSFIGLGAQPPLPEWGALM
                     SAGRNYLNNAWWLALFPGIAVTCTVVGLNLLGDGLRDLLDPRMGRSLNSD"
     gene            complement(7417..8346)
                     /locus_tag="VHS06_05155"
     CDS             complement(7417..8346)
                     /locus_tag="VHS06_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2987541.1"
                     /translation="MGYYLSRRIGGLLVTMLVVSFVAYVALTYAPGDAAEALAGENAS
                     AEQLAALRSQMHLDAPLAERYADYMTAALTRGDLGRSLISGRSVREMLEERIPPTLLL
                     AVVAMGLAAIAGVGSGLLAASRPGSKLDIVAMGGTALGLAVPPYWVALLLVLLFSLRL
                     HWLPVFGYGTAAHLVLPAITLALPGAAMVARLIRSSVVEVKGADFVRTAASKGLGPSQ
                     LMLRHILPNSLIPALTMLGLNLSHLIGGAFVIETIFAWPGLGRLTVQAIFDRDLPVLM
                     GAVLTVVPLCLTVNLLVDLLHGVLDPRLRHEAI"
     gene            complement(8494..>8786)
                     /locus_tag="VHS06_05160"
     CDS             complement(8494..>8786)
                     /locus_tag="VHS06_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987542.1"
                     /translation="PDALNIYLSSFRIRQTNRVFYSNPKVDALFEKGLREMDEAKRGQ
                     IYEDAQKQILADAPWQPLYYPMEGMVSRDRVQGLKVGAMGRMLVNDVTLSGK"
BASE COUNT         1598 a         2640 c         2806 g         1742 t
ORIGIN      
        1 gcgcctcgtc cttcaccgtc accctgccgt cggaatcgat ggtgacctcc atactggtcc
       61 catagccggc gttgaattcg tcgacgacgt tgtcgatagc ctcataaacc aggtggagga
      121 gcccggatgt ggaggtggtg ccgatgtaca tgccggggcg ctgccggatg tgctgaactc
      181 cctccaggat ctggatctga tcgccggtgt agctggccga gtcgtccgat ccgttgccgt
      241 tgccgtttcc gtttcttcca ttgccggcga cggcctcggg ttgtcccgag actgcagcgt
      301 gagagttggc tgtcgaaatg gcgggagcgg atcgctgctg tgcaggtatc tccacggaga
      361 cttcagaggc cggatcagca accgtcgtcg ctactctgcc gcctgtcgcg gcaccattcc
      421 tcaacgtggc ttttggttca tcgaagagag tgggcgtagc tgccccggtg gatgctttga
      481 cacggccggc agtcgtcttg gccgccgagg cagtcgactt cgtggtcggg gaggccggct
      541 tggctggggg aatgtccttc gcacccctgc tgggcttgct atccttggag gcggcggtct
      601 ttgggactgc ctttgtcttc ttcgcggcgc gcgccgaccc tgccttctcc ctggcctcct
      661 ggtcccacgc catcaccagt tgcctgggat ccgggtcgcg cggatccaga tccggatctg
      721 gggctggctg ggatttggaa ccgccgccga aaagcctgac taccaatgtc tacctccgtg
      781 gagatcgaag ctggactcga gaagcgtccc tccggttgga gcggccgcag gcacacgatg
      841 gagacgcgga gcacatggag agtgttggag tgcccacgtg ggacccgagt ctcggtggcc
      901 gtcgagggcg atggcagcat ggcgcgggct gcggtagccc atgtccatgc aagagctctc
      961 gctcgaacgc tggcatatta gcagaagagg cgcaagttcg acagtcctac ccatcctact
     1021 gccaggctga attgcagggg caagagcgac accaggtttg ccattagaac acgcgttcta
     1081 atactagcac ctcggttgcc ctttgtccag gctgtggggg agtcggactg ctactgatag
     1141 acttcgggtt tcccgcgaag aagaaccact gactcggcaa tagacaggat gcaatttcta
     1201 gatgcttatg gcattatgtg gcaaactgtt gaggcccctc cggatccgac acttttacgg
     1261 agaaatcccc ccttccggtt cggcatcacc tgaggagcgt tggacgtcat gactgacggc
     1321 tgtacccaat acgacgactt gcacggctcg attgacgagg tcatcgagaa gctcgactcg
     1381 atacggcact ggctgcgaac gtattccgaa tcccacgagg tctccggaag ggattcacgg
     1441 gacacgctag gcctgatggc aggctacgcg gagagcctgt ctcaggcgat gttcaaggtt
     1501 cagtcttcca taggggaaga gcgaagcgtc gcggcggatg caatctggac gctattagtg
     1561 gttacagggc aggtgcgcaa ctggctgatc tccaatggcc gtgcttcggc cctgtcccga
     1621 agggatcccg atactcagca gttgatgttg gcctatagcg tggtggcagg acagcaggcc
     1681 gatatcctca agaccattct tcgcgagtac aaagacggac agggctgacg gccgaaccgc
     1741 taccctgatc gacttctgct actgctgagc gccggcttct accggctcgg gggaccggcc
     1801 tgcgtggaat ttccagaagg agtcgaaggc gtaccagagc agaacgtatg cagggacctg
     1861 attcagtccc aacccctggg tgagaacctg gcgtagcccg agcgccccca aggttgcggg
     1921 aaacagcagc ttaaggtcca gccggcgtcc ggtaatactg gagagtcgac gatcaaggtc
     1981 gttcagggaa tcggtaatgg aagtggcggt agtgctcttg cctaccgctg gtagttcggt
     2041 tcccccggct tcgagaatgt cgcgcacgat cacccctgca tccttcagga tggagagtag
     2101 ttgatcgtgg ttcagttgct ccggatcgta gtggagcaga aggcttccag tcgtgctgtt
     2161 cccctctact tggcgaatcc cttctcgtag ctccagactg cggcgtgcct cgtgcagcga
     2221 ctcccggctg cgccggttcc ggtccagccg cacgcgcaat ctcccaggtg tgtggctggc
     2281 caccttggcc gaggcttcgt ttcccgtcca caccgtttcg ttggggttca ctgatcatta
     2341 cctcctgccg ccctgccgta tcgcgccgat gggatccgct tatggggcca aatctgactc
     2401 cagcaccatc tgtaccagca ggaccgggtt ggccaaggta tggcttctgc tttcgctgcc
     2461 ccctctgatg ctcgatattg ctcgaagaac ggagatggga tctcttggag ggcttctttc
     2521 agcttggagc accggcgtgg cagatcgttc tgagatccgt cgggatctat ctcgcgcttc
     2581 tgatcggact gcgactcttc gggaagcgtg aggtggggca gtttacgatc ttcgatcttg
     2641 ccttcgtgtt gctggtggcc aacgcagttc agcccgcgat gaccggcccg gacaattcct
     2701 tcgtcggtgg cgtcctgatt atcgccaccc tgctggtggc caactggtcg gtgggctggc
     2761 ttcggttgca cacgccggtc tttcgtgaag cgttcgagag ccaccccacg gtgatagccg
     2821 aggatggcag gtggtttacc gatgtgatgg acagggaggg ggtcaacctg caggaggcag
     2881 agatggcgct gcgggagcac ggcgtggaga acgttcaggg agcccggctc gtggtgctgg
     2941 aatccgacgg cggcatcagc gtcgtgccca gggagtcggg tggcaaggag ggaggccatc
     3001 ggcggagggt gagatacaga aagcagcaaa cgtgaaaatg gcccgcccat gtgcgcacag
     3061 gcgggccatt tctgtgtcta cggtgcctac ttcttcagcc gggagagcac ctcggggttt
     3121 accggggtga gcggggcctt gcctgccagg acggctagca cgttgtcggc ggccaggtct
     3181 accatcgcct ggcgagtcgc gacggtggcg cttccgatgt ggggcgcaac taccacgttg
     3241 tccatcttca gcagcggatt gtcggtaggg gttggctcct gctcgaacac gtccaggccg
     3301 gctccggcga tccgcttctc cttcagcgcc gcgatcagcg ctttctcgtc caccaccggc
     3361 cctcgagcgg cattgatcag gaatgcggtc ggcttcatct tcgcgaattg ggccgccccg
     3421 atgagtccct tggtctgggg catcagcggc atgtgaactg atacaaagtc ggactgggcc
     3481 agtagcgtgt ccatgtccac gtactggtaa ccgaactgct tttccagttc ttctctccgg
     3541 atcgggtcgt agtagagcac cgtcatgtgg aagcccgttg ccctgtgggc cacttcggcg
     3601 ccgatcctac ctaggccgat gatgcccagt gtggcgtggt tgacgtcgct gcccaggaag
     3661 gtggcgggac caccctgcat cgtccacttt ccgcctcgta cgaagcgatc ggcctcgcat
     3721 atgcgccgcg acaccgtgag catgagggtg aaggccaggt cggcggtggt gtcggtgagg
     3781 acgttggggg tgttggtgac aacgatgccg cgttgggttg cggcgggtac atccacgttg
     3841 tcgaacccca ctgccgtgtt ggcaataatg cggagcttgg gggccttgtc catgagtgcc
     3901 gccggccagg gggcaaatcc catgcacgcc tcggcatcgg caatcgctct ttccatctca
     3961 tcctgcagtg gacggctgtc ctcgtgccag aaggaaacct cgcattgggc ggaaagccgg
     4021 gccatggtgc tgtcgatcac cggcctggtg atgaagagct tcggcttgga catggatact
     4081 actccattat tgtttccgcc ggagtcactc cggcggaaat cgagtctgcg gccactactt
     4141 gggcagtctg tccaggatca ggtcgatata gcgcagccga ggcaggacga ggaatatcgc
     4201 gttgaccgtg ttgtcactat gggcgaagtg agtgtcgatc acgaacacct catcgttctc
     4261 cgtggccagg tccgcgagca cggtgtccat aaccgcgcct gccatgtcaa cggccaccgc
     4321 cggcggggag ggggagatct ccaaccctgt gctgtcggcc agggcacaca ggaagaagga
     4381 gcaggtaatg ttgccgacct ctccaagagc cgatcgggcc atgtcggaca gttcggcacc
     4441 ggcctcctca ggctcgccca agaccatgga gaccagcctt agggcgtcct gcatcggcag
     4501 cagcagcagc atgtggccct cgacgtctcc cacgatcttc acgtaggcgg cggcggtgag
     4561 gatctcctct ccgccgacca gctgcggcac ctgcggcatc ggcaccttcc tgacctgggc
     4621 cgccttcgcc tcgatgggag tgccggtcat ctcggatagg ccgcggctcg atctctgaaa
     4681 cccaatatcg gccacaacgg agagcgccct gagcacatcg ctcaggcggc ggccacggtt
     4741 ggtggttcgc cgatcctctt cggttccgcg ggtcaccgat actctgcttt cctcgttcat
     4801 ttttcctccc cgatcccctt acggttggtt gccggtagtc ccaccccgat cgcagccgtt
     4861 gtctccgcta ttgtggaaga cgggataggc aagcaaaacc aggacaaaca gacccactgt
     4921 ggaagggacc gaaagacgcg aaagatggaa tgcctggggc ggatagatac ttccaagcac
     4981 agtctatcac tacttgcacg atggcgaaac tggtagaaat cgccaaatgc gcccattcca
     5041 aggggcctag tcctcggcca ctatggcgag ctcggtggct gtcaaacgga acagtcgcca
     5101 ctcggacatc tgcttggcgc ccagcccctc gtagaaggcg atggccgttt tgttccaatc
     5161 gagtgccgac cattccatcc ggccgcagcc tcgccggacc gcttcgcgcg ccagttctct
     5221 catgagcatt cggccgtttc cgttgccacg ttcactcggt agaacgaaga tgtcctcgac
     5281 ccaaagtgtg gggcgcgcga ggaaggtgct gtaggtgaag aagaacaggg agtagcccac
     5341 caaccggccg tcctgttctg ctaagagcac gtggaagacc ggcgggtcgg ccagcgcgtc
     5401 ctgtgccatt cgcgcacgag cttcttcgtc gggaccgggc aggctttcgt agtcggcgag
     5461 cccctgcacc aggtcgagga aagttggaat gtccttctcg gtcagggggc gaatgtgaag
     5521 tgtcatgggc ggcaaacctc cttttggctg gcagatcgcg ctggctggca atgatagtcc
     5581 aaagacgcac cacgcgcacc ggcgcctgtg tggacctgcg acctcggtgc cgctgattag
     5641 gtctcttagt tgtcaccctg agcgcagcga agggcctcta cgtacattcg aggagattct
     5701 tcgctgccgc tttcagtgac agcccggaaa cgggaagcgc catccgcctt tcggtttgtc
     5761 gtatactgtt gagtcgggac tcagagttgt tcggcgagga gagggatgct tccagacttg
     5821 cccaccctgg ccctattcat gttcgcgacg ctgatgctga atgtaacccc cggaccggac
     5881 atgctgtacg ttgtcgccaa cagcgtcggc cgaggccaga gggctggcgt cgtctctgct
     5941 ttcggaattg gtgccggcac cttggtccac accctcgcgg ctgccttcgg cctgtccgcg
     6001 ctgttaatgt cctcttctct ggccttcgac gttgtccggt atggtggcgc agcctacctg
     6061 atctatctcg ggatcaggtc gctcaccggc agggagagcc ccatcccctc aggccgactg
     6121 gagcgggagg gtttgggcaa gatattcaga cagggcatgg cgacgaacgt cctgaacccg
     6181 aaggttgccc tcttcttcct tgctttcctt ccgcagtttg tggatcctgc gcgaggctcc
     6241 gtcgtcgtgc agttcatcct gctaggtacc ctcttcaaca cttcgggcac tactgtgaat
     6301 agcctggtag cggtggcgtc gggctacgcg ggggagcgac tgggcaagag ccccggcttt
     6361 gccaggacgc agcgctggtt ctctggaggc gtcttcgtgg cgctgggcgc ccgcatcgcg
     6421 ctcaccgggg cgcggtagtc tagtcggagt tcagggacct ccccattcgc gggtccaaca
     6481 ggtctcgcag tccatcaccc aacagattca gtcccaccac ggtacaggtc acagcgatgc
     6541 cagggaagag agccagccac caggcgttat tcaggtagtt gcgtcccgcc gacatcagcg
     6601 caccccactc cggcagcggg ggctgagccc caagaccgat gaagctgagg ccgctgccgg
     6661 tcagcagagc cgtacccatg cgcatggatg tgagtaccac gacaggggag aggatgtttg
     6721 gaaagacgtg acgccacatg acccgccgat catcggctcc cagggcgatg gctgcctctg
     6781 catagggcat gtggcgagcg ctcagggtag tgttgcggac gatgcggtag aaggcaggga
     6841 ctcccatgaa tcccagcgcc aggatcaggt tgtccagtga ggctcccagc ctggcgacga
     6901 tgactatggc caccagtgcg cctgggaggg acagccaacc atccatggcg cgggagatca
     6961 gttggtcggt ccagccggag tagtatccgg ctaggctgcc caggatcagg ccgatcgcga
     7021 gcgagatgcc cacgctcatc acggctatcc gtgccgcgag ccatgctccc gccgcgaccc
     7081 tgatcagaag gtcgcgaccg agggcgtcgg tcccgaaggg gtgagtcgcg ttgggaggga
     7141 gcagcctcga gttgggtagg atctggtcgg gggtgtatgg acagaacaac ggcaccacta
     7201 cggcgccgac ggctatcatg gcgatcagag ccacgcccag gtagagggcg ggcgtgcgaa
     7261 ggaagttcct tttggcgggc gtccaccagc tgtcgtcggc ggtggaacgc cttctcggaa
     7321 tgggacacgg tgcctggggg ctccagatga ggcgaagcag accgtggatc cgccaggcga
     7381 tgcttgtgga tgtcgtgacc gggttgcgcc tctccatcat attgcctcgt ggcgcaagcg
     7441 gggatccagg acaccgtgca gcagatcgac gagcaggttg acggtcaggc agagcggtac
     7501 cacggtcagc accgcaccca tcagcacggg aaggtctctg tcgaagatcg cctgcacggt
     7561 caatcggccc agtcccggcc aggcgaagat cgtctctatg acgaaggcac cgccgatcaa
     7621 gtggctgagg ttaagaccca gcatcgtgag ggccgggatc aggctgttag gcaggatgtg
     7681 gcgcagcatc aattggcttg gccccaaccc tttcgaggcc gccgtccgga cgaaatcggc
     7741 ccccttcacc tcgacgacgc tggagcgtat cagtcgggcg accatggcgg cgccggggag
     7801 cgcgagcgtg atcgccggca gaaccaggtg cgcggcagtg ccgtatccga agaccggcag
     7861 ccagtggaga cgaagggaga agagcaggac gagcagcagg gcgacccagt agggtgggac
     7921 ggcgagcccc agcgcggtgc cgcccatggc cactatgtcc aatttgctcc ctggcctaga
     7981 cgcagccagc agccccgatc cgactccggc gatggcggcc agccccatcg ccactaccgc
     8041 aagcagcaac gtgggcggga tgcgctcttc cagcatttcg cgtacggatc gcccgctgat
     8101 caacgatcga cccaggtcac cgcgggtgag ggcagcagtc atgtagtccg catagcgttc
     8161 cgccagcgga gcgtccaaat gcatctggga tcgcaacgca gccagttgct ccgccgaggc
     8221 gttctcgccg gcaagtgcct ccgctgcatc acccggcgcg taggttagcg caacgtaagc
     8281 gacgaaactg acaaccagca tcgtcaccag cagcccgcct atacgcctgg agaggtaata
     8341 acccattgcc tggtgctcca tcacgcggca tccgacggat gatagggact ctcctgtcaa
     8401 cgcacggtag gggcgcacct gcgtgtgcgc ccaggactga gggcggacac acaggtgcgc
     8461 ccctactatc cgtcgaacgt cgtcgaaacg acactacttg ccgctcaacg tcacgtcgtt
     8521 taccagcatt cgccccatcg cccccacctt tagcccctgc acccggtctc gggaaaccat
     8581 gccctccatg gggtagtaga gcggttgcca gggtgcgtcg gcgaggatct gcttctgggc
     8641 gtcctcgtag atctgcccgc gtttggcctc gtccatctcc ctcaacccct tctcgaacag
     8701 ggcatcgacc ttgggattgg agtagaaaac cctgttggtc tgccggatcc tgaacgagga
     8761 caggtagatg ttgagggcat ccggat
//
LOCUS       DATALS010000232         7545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_417674, whole genome shotgun sequence.
ACCESSION   DATALS010000232 DATALS010000000
VERSION     DATALS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417674"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VHS06_05165"
     CDS             <1..320
                     /locus_tag="VHS06_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987543.1"
                     /translation="PAIRPGGERGVAWVVGGKYGHRGANGPMVGFVLASFVPAVGPGY
                     SEVVQLHRRFLDLFADYGRRKERVGDMVVRLGLRTVLELMELDPDPAVLDEPTHQLFI
                     VWP"
     gene            complement(706..1662)
                     /locus_tag="VHS06_05170"
     CDS             complement(706..1662)
                     /locus_tag="VHS06_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987544.1"
                     /translation="MRLNRSQWGMGGYVSIPPLLSGVSVIGSNRPELSGEITGIRLLG
                     VPGGFLSPSLMRGLADIADEYGDGRLRWTTAGSIEIFVGRDRMLDAVEAANRLGMDVG
                     STGDDVRNICACPGSFRCDLALVNAPEIAFALGSAAIDDQQYPGLPNKVKSGVSGCPN
                     DCCRVQMEKDHAFVGVFRGAPEVDDSLMDWWQREGRFGGGSMGSSPMADLDLLIRKCP
                     GNAIERAGDSAVIDAASCRHCMMCISRCPAIRPGGERGVAWVVGGKYGHRGANGPMVG
                     FVLASFVPAVGPGYSFTRQSTLDGSTPTNSKALREVLRPTRA"
     gene            complement(2608..2952)
                     /locus_tag="VHS06_05175"
     CDS             complement(2608..2952)
                     /locus_tag="VHS06_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2987545.1"
                     /translation="MKVYTADSVAPSQVPHGEGVAIRWLITTEHGAPNFAMRIIEVQP
                     GGGTPFHTHPEEHEVYVLEGKGALLDEEEKEMALAPGDVAFVLPNEQHQFVNRGDTML
                     RFICVIPLLKRE"
     gene            3254..3622
                     /locus_tag="VHS06_05180"
     CDS             3254..3622
                     /locus_tag="VHS06_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987546.1"
                     /translation="MAKILVVDDDNYLRGMSVRLLQKAGYQVVEASDGVDGVTKFEDE
                     QPDAVLMDINMPRMDGLSALVAMRRINPKARVAMLTGLAEKKVALTAVQMGARDFVLK
                     PYKVERVLLAVQRLLGARQE"
     gene            3631..4338
                     /locus_tag="VHS06_05185"
     CDS             3631..4338
                     /locus_tag="VHS06_05185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2987547.1"
                     /translation="MSMPRALLFDLYDTLIWLDTDKSNRYRQQFATRIGLSLEQFLRH
                     WRQSIDHRMLGKGEGLPGHLATALSEMGIEPEPELISDLVEIERERLEQCAHPYPSTF
                     PVLNRLSAEGYTLGLLSNVSDGAAIPITFLGIDKLFDRMVLSHEVGLLKPDPAIYRLA
                     CEQLDVAPTETVFVADGGFGELDASYEMGIFSVMLEQDNQSRDYGFSTRYHAKIHDLS
                     ELESLLSRLRDGDSTGG"
     gene            4331..4951
                     /locus_tag="VHS06_05190"
     CDS             4331..4951
                     /locus_tag="VHS06_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987548.1"
                     /translation="MASALQLVRQHRLTLILLNVGFFSLVALGTIYAFANPSAQQTLI
                     QTIVEGFGTQPLNWARDAYLSRSVPAAAAVTFAVNSLLGSFLSITVTSLLIPFLGVAV
                     GFYRALVWGLALAPTSPELAWAMIPHSVTLVLEGEGYILAMFGVHLLWQSAFKGLQEG
                     GPRGLLAGYKAGLRANLHIYILVLLVLAVAAIYEAVELIYIVAGSR"
     gene            5019..6365
                     /locus_tag="VHS06_05195"
     CDS             5019..6365
                     /locus_tag="VHS06_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX2987549.1"
                     /translation="MLPSGFKRWLLIQAAFLLVAAAAASPHTYAQGDHVAVLSIKGII
                     NPVAAGYVERALNEAESNGATAAIIEMDTPGGLDTSMRAIIQRIIAAKIPVIVYVSPQ
                     GARAGSAGVYITYAAQLAAMAPNTNIGSAHPVAMGDNGNEQQLSDTMAEKITNDAVAY
                     IKGLAQTRGRNAEWAEKAVRESVNVTAQEALDLKVIDLVADDMNSLMDQVDGREVQLS
                     SGKVTVRTRGLPLERISMDAMESLLHTVSDPTIAYILLSLGTLGLVFELSNPGAILPG
                     VVGGISLLFALFGLGTLPVNIAGVMLIGFAFLLFVVDIFAPTHGVLTGGGVISFVLGS
                     MMLINTRNAPFLAISTSAIVAVSAALAGFFGFVVGAVVRSHRRRPVTGEAGLIGKVGR
                     VKSSLEPGGMVFVDGELWRAISESGPIAEGELVEVVSTRGLTLVVVPHPTEKQLRS"
     gene            6643..7329
                     /locus_tag="VHS06_05200"
                     /pseudo
     CDS             6643..7329
                     /locus_tag="VHS06_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
     gene            complement(7242..7523)
                     /locus_tag="VHS06_05205"
     CDS             complement(7242..7523)
                     /locus_tag="VHS06_05205"
                     /inference="COORDINATES: protein motif:HMM:NF039222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987550.1"
                     /translation="MGNGIAIVTNAGGPGIMASDAAERLGMKIAHLTPEITEKLQAAL
                     PPSASAPVWEEIAREPAAVVPDLMIRTGFCLVTLRAVSMNFWPLVMPSR"
BASE COUNT         1468 a         2345 c         2296 g         1436 t
ORIGIN      
        1 gtccggctat acgacctggt ggcgagcgag gggtggcatg ggtggtcggt gggaagtatg
       61 ggcaccgcgg cgccaacggg ccgatggtgg gcttcgtcct tgcttccttt gtcccggcag
      121 ttgggcccgg ctactccgag gtggtgcagc tacacagacg gtttcttgac ctcttcgccg
      181 actatgggag gcgtaaggag cgggtgggcg acatggtggt gcggctcggt ttgcgtactg
      241 ttctggagtt gatggagttg gatccggacc cggcggtgct ggatgagccg acccaccagc
      301 tgttcatagt gtggccgtag gtgtcggctc aatgacggat cgagtgatgg taggggcgga
      361 cctgtgtgtc cgccctcggc tctgagcgga cacacaggtc cgcccctacc gtgcgttgac
      421 gggcgggtgc cgattaccgg ctattggata gacactgtgt gtgggctcgt gaggaatgaa
      481 gaggtctagt ctccctgttg ggatcatgcc cgatccgaag gcgcagcttc ccgggttgct
      541 gttgcggaac gaggggaagt gggttcgcca cgagggcgtg cggcccgggg tatatgggca
      601 tgtctcctcg accggtgagg cgtgtttgac ggtgagggca cagatgcccc ccaacggatt
      661 gctctcgtcc ggcagctatc gaaggttggc atcgctggtc gagagttatg ctctcgtggg
      721 gcggagaacc tcccgcaacg ccttcgagtt ggtcggggtg gatccatcga gagtagactg
      781 cctggtgaag gagtagccgg gcccaactgc cgggacaaag gaagcaagga cgaagcccac
      841 catcggcccg ttggcgccgc ggtgcccata cttcccaccg accacccatg ccacccctcg
      901 ctcgccacca ggtcgtatag ccggacagcg actgatgcac atcatgcagt ggcggcagga
      961 cgcggcgtcg atgacggcgg aatctcccgc cctctcgatg gcgttgcccg ggcacttcct
     1021 gataagaaga tcaaggtcgg ccatggggga cgaccccatc gagccccccc cgaatcggcc
     1081 ctcccgctgc caccaatcca tcagggaatc gtccacctca ggggcacctc tgaacacccc
     1141 gacgaaggcg tggtccttct ccatctgaac tctgcagcaa tcgttgggac agccggacac
     1201 cccactctta accttgttgg gcagccccgg atactgctgg tcatctatcg ccgcactacc
     1261 cagtgcgaag gcgatctccg gggcattgac caacgcaaga tcacagcgaa aactcccagg
     1321 gcaggcgcag atgttgcgga cgtcgtcccc tgtggacccc acgtccatcc ccagcctatt
     1381 cgcggcctcc acggcatcca acatcctatc gcgcccgacg aagatctcga tactcccagc
     1441 cgtcgtccac ctcaaccggc cgtcaccata ctcgtcggcg atgtccgcca gccccctcat
     1501 gagagagggc gacaggaacc ctcccggcac cccgagcaat cttatgccgg taatctcccc
     1561 gctcaactcc ggccggttgc tcccgatgac ggacacaccc gacagcagcg gagggatcga
     1621 tacgtagcca cccatccccc actggctcct gttcagccgc aacccctcct ccagcatcgg
     1681 catcgggaat cgggtctcac gcaactcccg gagatggctc ggatatcggc cctccgtcgc
     1741 gcagttctcg cccgccgatg accgcaatgg ctgctgctct gcagatccca cgttatgccc
     1801 gagtccccct tggtctggtg ccgcccagca caatagagct ccacgagcgc ggactaagtg
     1861 gcgtatccgg caagtcccat ggccagccag cgctagcccg agtctatgcc ccgacttcgc
     1921 tcaggagaat aggtggaatg ccccaaaacg cgatgcataa aacgccctat cttccccgtc
     1981 cggcccccct cgcgtcatca aagatgccat acacgcttcg ggcacctccg accctacgtt
     2041 ggcgctctga tcggcgcatt ttgacgcccc atcgctccgt tggcgccaca aatggcgctc
     2101 cagaaaaccg ctatcggccc atttggcgcc ttttctcggc ctcacgatag cgccatcgac
     2161 cccgcaggcg cttcgccaag ggtcgaattg gcgggttctg cgggccgtgg ttggagcaac
     2221 caggatgaga tctggcatca ggaacgggcg ttcttcggaa gtcagccttt ctgaagacgg
     2281 ggccgcaagg atggcatcgg aggcagacgg cccgtgggat ggaaaggcag gcagggctga
     2341 agaacgagaa ctgcagagaa gaccggggca ggaggagaag ctccggccat agcagaatga
     2401 aacggtcggg gaaatggagt agagacttca gccaagaccg tgagggatac tccggccgaa
     2461 gtctctactc caccgggctg actttcgtcg gacagccctc gggcgtgccc tccagctatc
     2521 cgctgctgca cgagaacact ctgcaactag cgccgacact cgtgccgtgc catcccctcg
     2581 aatgggtatc tgcgtgtact gtcgacgcta ctccctcttg agcagaggga tgacgcagat
     2641 gaagcgcagc atcgtgtctc ccctgttcac aaactggtgc tgctcgttgg gcagaacgaa
     2701 ggcaacgtca cctggggcca gggccatctc cttctcttct tcatccagca gagcgccctt
     2761 gccttccaga acgtacactt cgtgctcctc gggatgggtg tggaacggag tcccgcctcc
     2821 tggctgcacc tcgatgatgc gcatagcgaa gttgggcgca ccatgctccg tggtgatcaa
     2881 ccatctgatg gcgacgccct caccatgagg aacctggctg ggtgctacag agtccgcggt
     2941 gtagaccttc atctcgacct cctgaaagaa tgggggtaca gggaaagagg gaaccacgtc
     3001 gatgccccag tccagagttg ggcggtctgg cttcggcaga tccgagaccg acccaactca
     3061 caggcgcttg tgcgcatgtc tcagtctaca gcgcttcccg catcgtttcc accctataat
     3121 atggtagtgc cacactagac tgtctcccgg tccaggatgt accattaagc gtgtagtgtg
     3181 acatcatcct tcccatggag atcaacggcg ggccagtaag cggtcggaaa ctgccttatt
     3241 ggagtcttta tagatggcaa agatcctcgt ggtcgacgac gacaactacc tgcgtgggat
     3301 gtcggtcaga ctactccaga aggccggcta tcaggtagtc gaagcgtcgg acggcgtcga
     3361 tggagtgacc aagttcgagg atgagcaacc agacgcggtg ctgatggaca tcaacatgcc
     3421 gcggatggat gggctcagtg cacttgtcgc gatgcgccgt atcaacccca aagctcgggt
     3481 agcgatgcta accggactcg ctgagaagaa ggtcgcgctc accgccgttc agatgggagc
     3541 acgcgacttc gtactcaagc catacaaggt tgagagggta ctactcgcgg tccagcgact
     3601 gctgggcgcc cgacaggagt agtctctgtc atgagcatgc ccagggcgct tctcttcgat
     3661 ctatacgaca ctctgatatg gctggacacc gacaagtcca accgatacag gcagcaattc
     3721 gccaccagga ttggcctctc gctcgagcag tttctgcgac actggcggca gtcgatcgac
     3781 cacaggatgc tcggcaaggg ggaagggtta cccgggcacc ttgccacagc cctttccgag
     3841 atgggcatcg agccggagcc tgaactgatc tccgacctgg tcgagatcga gagggagcga
     3901 ctggagcagt gtgctcaccc gtaccccagc acattcccgg tcctcaatcg gctatcggcc
     3961 gaaggctaca ccctgggtct gctaagcaac gtctccgacg gggcagccat acccatcacc
     4021 ttcctgggca tcgacaagct attcgacagg atggttctct cccacgaggt cgggctgttg
     4081 aaaccggatc ccgccatcta tcgcctcgcc tgcgagcagc ttgacgtcgc ccccaccgag
     4141 acggtcttcg tcgccgacgg tggtttcgga gagttagacg cgtcatacga gatggggatc
     4201 ttcagcgtca tgctggagca ggacaaccag agccgcgact atggcttcag cacacgttac
     4261 cacgccaaga tccacgacct gtccgagttg gagagtctac taagccggct tcgggacgga
     4321 gactccacag gtggctagcg ccctccagtt ggtccgacag cacaggctca ccctgatcct
     4381 gctgaacgtc ggcttcttct ccctggtggc gctgggaact atctacgcct tcgccaaccc
     4441 cagtgcccag cagaccctca ttcagaccat cgtggaaggc ttcggcacac agcccctcaa
     4501 ctgggccagg gatgcctacc tcagccgaag tgttcccgca gccgctgccg tgaccttcgc
     4561 cgtcaacagc ctcctgggct cgttcctgtc cataaccgtc acctccctcc tgattccgtt
     4621 cctgggcgtt gcagtcggct tctaccgagc gctggtatgg gggctggcac ttgcgccaac
     4681 atctccggaa ctggcgtggg cgatgatccc ccactccgtc accctcgtac tcgaggggga
     4741 aggctacatc ctggcaatgt tcggggttca cctcctttgg cagtcggcat tcaagggact
     4801 acaggaaggc ggaccgcgag ggctgctggc cggctacaag gcggggctca gagccaacct
     4861 ccacatctat atcctggttc tgctggtgct cgcagtcgcc gcgatctatg aggcagtgga
     4921 actgatctac atcgtcgccg gttcgcggta accgacggtc gagggccgac agtagactgc
     4981 tttgtgctat gatagccctc tggaagggtg ggggtttgat gctgccttca ggcttcaaac
     5041 gttggcttct gatccaggct gctttcctcc tggtggcggc agcagccgcc tctcctcata
     5101 catatgcaca gggcgatcac gtcgcggtcc tttcgatcaa ggggatcatc aatccggtcg
     5161 ccgccggcta cgtcgaacgg gccctgaacg aggccgagtc caacggcgcc accgctgcga
     5221 tcatagaaat ggacaccccc ggcgggctcg acacctccat gcgggcaatc atccagcgga
     5281 tcatcgccgc caagataccg gtgatcgtgt acgtctcgcc tcagggagcg agagccgggt
     5341 ccgcgggcgt ttacatcacc tacgcggctc aattggccgc catggcgccc aacaccaaca
     5401 tcggctccgc ccacccggtg gccatgggag acaacggcaa cgagcagcag ctgtcggaca
     5461 ccatggcgga gaagataacc aacgacgccg tggcttacat caaggggctg gcccaaacga
     5521 gggggcgaaa cgcggagtgg gctgaaaaag cggtccgcga gagcgtcaac gtgaccgcac
     5581 aggaagcgct cgacctcaag gtgatcgacc tggtggccga cgacatgaac tcgctgatgg
     5641 accaggtaga cggcagggaa gtccaactgt ccagcggcaa ggtcaccgtg agaacccgag
     5701 gtctccctct ggagagaatc agtatggatg cgatggagtc gctcctccac acggtaagcg
     5761 atcccaccat cgcctacata ctgctgagcc tcggaacgct ggggttggtc ttcgagctgt
     5821 cgaacccggg agccattctt ccaggtgtgg tcggtggcat ctccctcctc ttcgccctct
     5881 ttgggttggg cacgcttccg gtaaacattg cgggcgtgat gctaatcggg ttcgcgttcc
     5941 tgctgttcgt cgtggatatc ttcgcgccca cccatggtgt cctgacggga ggcggggtga
     6001 tatccttcgt gctgggatcg atgatgctca tcaacacccg caacgccccc ttcctggcca
     6061 tatccacgtc cgccatcgtg gccgtgagcg cagccctcgc gggcttcttc ggcttcgtcg
     6121 tcggggccgt cgtcaggtct cacagacgca ggccggtaac aggcgaggcg ggcctcatag
     6181 gcaaagtggg tcgggttaag agcagcctcg aacccggcgg catggtattc gtggatgggg
     6241 agctatggag ggcaatcagc gagtctggtc ccattgctga aggtgagttg gtcgaggttg
     6301 tatccacccg aggattgacc ctagtggtgg tgccccatcc cactgagaag cagttgagaa
     6361 gctgagaaga agcgtgttat ccttctggaa actggcgggc atatggccta gttcttgctc
     6421 gccaaatcta catagttgtc cctttacccg cgtgaaccca aaggagggct gtacccttga
     6481 tggaagacgt gggtagtcgc agacaatcga cgtagtagaa actgcaagga ggcagggagc
     6541 agcgtgtcag acaccgtttc gaagccgaaa tacgttgtgt tcgcaaagga gtcgggagag
     6601 tcagaggcca aggtgctaga cgtcttcttt aggccgaaat ccgtggccgt ggtcggagcc
     6661 acccgagacg tcaagggtct gggcggcagc gtcctggaca acatcatcaa ggcaggctac
     6721 accgggcccg tgttccccat caaccccaag gccccagaga tcgcagggtc gaaggcatac
     6781 ccctcggttc tggacgttcc ggagccggtt gacctggcag tggtcaccac tcccgctcgg
     6841 ttcgttcctc agatcgtcga gcagtgcggc cagaagggct gccgcggcat gatcatcatc
     6901 agcgctggct tccgagaagt gggagccgag ggccggaaga tggagaggga gatgggtgaa
     6961 gtagccaagc gctacggcac ccgcatcgtc ggccccaacg ttctgggtgt cgtggatacc
     7021 ttctccccct tgaacgcaac cttcgcccca tcgatgccgg gccagggccg aaccgccttc
     7081 atgtctcagt ccggcgcact gctgtccgcc atcctggact ggtcgctggg tgagggcttc
     7141 ggcttctcca gcctggtctc cctgggcaac aagctggacg tcgacgagaa cgacctgctg
     7201 gaagcgtggg tcgaggacga taacaccaag gttatcctgg gctacctgga aggcatcacc
     7261 aacggccaga agttcatcga gactgcccgc aaggtcacca agcagaagcc tgtcctgatc
     7321 atcaagtccg gcaccacggc agccggttcc cgtgcgatct cttcccacac cggcgcgctg
     7381 gcggagggcg ggagcgccgc ctgcagcttc tccgtgattt caggggtcag atgggcaatc
     7441 ttcatcccca gccgctcggc agcgtcgctg gccatgatgc cggggccgcc ggcgttggtc
     7501 acgatcgcga tgccgttccc catggggacc ggctggttgg cgaaa
//
LOCUS       DATALS010000233         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_381154, whole genome shotgun sequence.
ACCESSION   DATALS010000233 DATALS010000000
VERSION     DATALS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381154"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1067
                     /locus_tag="VHS06_05210"
     CDS             252..1067
                     /locus_tag="VHS06_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEX2987551.1"
                     /translation="MAIITISRGTFGGGEALAEALASRLGYECVSREALVESATWYGV
                     PAGSPGDAENRAGSLERLASERKAHLVSVRAALCDRARGGNLVYHGHAGHLLWGEDVS
                     HVIRLRVVADMEYRIRAAMKRHSLGREGAIAYIEKVDRERSEWTNFLYGVAWEDPSLY
                     DMVLNLARISIPSACEIVAQLTQLPEFRPTPRSMKAMEDIALSSRVLAHLLRDPRTAG
                     LDLKVTADDGRVTINCAAQPRDAQDAIPIVAAQVEGVKQIDCSPVPTVGSSPQ"
     gene            complement(1120..1488)
                     /locus_tag="VHS06_05215"
     CDS             complement(1120..1488)
                     /locus_tag="VHS06_05215"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HEX2987552.1"
                     /translation="MGDKLGSRAPRIFYVNWFRKTREGKWLWPGYGENSRVLKWMCDR
                     VDGRVDAVNTPIGLLPNPSDLELAGLDISQEELTELLRVDKDAWRSEIPDIEQHFAKF
                     GDRMPARLTSQLYDLQTRLG"
     gene            complement(1391..>2087)
                     /locus_tag="VHS06_05220"
     CDS             complement(1391..>2087)
                     /locus_tag="VHS06_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987553.1"
                     /translation="TLAGKTLQTTGAVLSATGAACWNPHTRSVGFMFATLGLSLSTRL
                     FDRPTVARTSLCAVLSASALAAHMELGLFVAYSLLLLAITHKRRVHALSLAALLLVLM
                     VAVSSPWWLTVLGRHGLAPFDAASLTSGWSSPIDRLETLERYLFLPGPFFGVLGASAL
                     LGVIICIIRGQLFLPLWPLVVFLMTPRSAESEGALPLARDGGQTRESGTQDLLRQLVP
                     EDPGGQVAVARLR"
BASE COUNT          418 a          599 c          689 g          381 t
ORIGIN      
        1 tcgcttgtgg gtgatggcca gcagtagcag gctataggcg acgaagagcc ccaactccat
       61 gtgggtagcc aacgctgagg cagataggac agcgcagaga gaagttctgg cgactgtgca
      121 ttagtagttg ttggttcccc acccataggc cagtggcgag accggcccga tgatttgcct
      181 cagggcgggg tagtttcctg ccggcgcatc gctatcggtt ctggacccat cttttccgca
      241 aggagggggc aatggcgatc attaccatat cccgaggcac gtttggcggc ggtgaggcac
      301 tagccgaggc tctggcaagt cgacttggct acgagtgtgt tagccgcgag gcgcttgtcg
      361 agtcggcgac ctggtatggg gttccagcag gcagccccgg tgacgctgag aaccgcgccg
      421 gtagtttgga gcgtcttgcc agcgagcgta aggcccacct cgttagcgtc cgcgcggcgt
      481 tatgcgatcg tgcgcgggga ggcaatcttg tctatcacgg acatgcgggc cacctactct
      541 ggggtgagga tgtatcgcac gtcatacgcc tcagggtcgt cgcggacatg gagtaccgga
      601 taagggccgc catgaagcga cacagtctgg ggagagaggg cgccatcgcc tatatcgaga
      661 aggtcgacag ggagcggagc gagtggacca atttcctgta cggcgtcgcg tgggaggatc
      721 cgtcccttta cgacatggtt ctgaatctcg ctcggatcag tattcccagc gcttgtgaga
      781 tagtggctca gttgactcaa ctgccggagt tcaggccgac tcccaggtcg atgaaagcga
      841 tggaggacat cgccctgagc agccgcgtcc tcgcccacct gcttcgggat cctcgcaccg
      901 caggtctgga tctgaaggtg acggccgacg acggtagggt cacgatcaac tgcgccgcgc
      961 agcctcgcga tgctcaggac gcgatcccca tcgtggccgc gcaggtggaa ggcgtcaagc
     1021 agattgactg cagccctgta ccgaccgtgg gctcctcacc tcagtaagag attacggccg
     1081 gggcgacgcg ttcgtcaccc cggccattcg gttgttaccc taccccaagc gggtctgcag
     1141 atcgtatagc tggctggtga gccgagccgg catgcggtcc ccgaatttcg cgaagtgttg
     1201 ttcgatatcg gggatctcag acctccaggc gtccttgtcc accctgagca gttccgtcag
     1261 ctcttcctgc gagatgtcca ggccggcaag ctccaggtcg gatggattgg gcaggagacc
     1321 gataggggtg ttcacggcat ccacccttcc gtccacgcga tcgcacatcc acttcaggac
     1381 gcggctgttc tcaccgtagc ctggccacag ccacttgccc tcccgggtct tccggaacca
     1441 attgacgtag aagatcctgg gtgcccgact cccgagtttg tcccccatct cgagccagtg
     1501 ggagcgcccc ctcagattca gcacttcggg gggtcattag gaagacgaca aggggccaga
     1561 ggggcaggaa cagttgccct cttatgatgc agatgatcac acccagaaga gccgaggcgc
     1621 caagaacgcc gaaaaagggg cccgggagga aaaggtagcg ctccaatgtc tcaagcctat
     1681 ctatgggtga ggaccatccg ctggtgaggc tggcggcatc gaaaggcgcg aggccgtggc
     1741 gaccgaggac tgtgagccac catggagagg agactgctac catgagcact agcagcaggg
     1801 cagcaagcga cagtgcatgc actcgtcgct tgtgggtgat ggccagcagt agcaggctat
     1861 aggcgacgaa gagccccaac tccatgtggg cagccaacgc tgaggcagac aggacagcgc
     1921 agagggaagt tctggcgact gtcggccggt cgaagagccg agtggagaga ctcaggccca
     1981 gcgtcgcaaa catgaagccc accgacctgg tgtgggggtt ccagcaggca gctccggtgg
     2041 cgctgagaac cgcgccggta gtttggagcg tcttgccagc gagcgta
//
LOCUS       DATALS010000234         7208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_24552,
            whole genome shotgun sequence.
ACCESSION   DATALS010000234 DATALS010000000
VERSION     DATALS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24552"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1611
                     /locus_tag="VHS06_05225"
     CDS             379..1611
                     /locus_tag="VHS06_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX2987554.1"
                     /translation="MEEQLYTQVIELEGHIIDSMMLPQIMDEVMDRGGDFEVQEIRVG
                     RKKFETSYARLEIRANSAALLDRLVANARRIGATVPAVEQVQAEPAPADGVFPEGFYS
                     TTNLETQVRLNSEWVTVANPEMDLGIVVDFEAGTARNVPIADVREGELVVVGHQGVRV
                     IPLARSREEKETFSFMGSAVSSEKPKANLIREIATEMKAIKARGGKTLVVSGPAVTHT
                     GAAPLMVRLIEAGYVNYLFAGNALATHDIEGAFFGTSLGISLSQGTPMEGGHQHHLRA
                     INRIRRAGSIKAAVEQGMLTRGIMHACVKNGVDFVLAGSIRDDGPLPDVITDVIQAQR
                     RMRSVIQQGVDLVLMISTMLHSIAVGNLLPATVTTVCVDINPAVVTKLADRGSFQAIG
                     LVADAEGFLRHLTTELAI"
     gene            complement(1658..1969)
                     /locus_tag="VHS06_05230"
     CDS             complement(1658..1969)
                     /locus_tag="VHS06_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX2987555.1"
                     /translation="MSFVDKSLRCRECGREFVFTAGEQEFYQLKGLLNEPARCPECRS
                     MRKAQRQGQDGRGMDARPAREMHPAVCADCGAQTLVPFLPRGDKPVYCSTCFDRVRSG
                     Q"
     gene            complement(2242..2457)
                     /locus_tag="VHS06_05235"
     CDS             complement(2242..2457)
                     /locus_tag="VHS06_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987556.1"
                     /translation="MKPMAGGRDRAAKYCDFLQSVRDNSQAKGDPEGRPIFRCQRRPG
                     IQLHSSYVSEVCSTPDHHECPFFDDSE"
     gene            complement(2832..5072)
                     /locus_tag="VHS06_05240"
     CDS             complement(2832..5072)
                     /locus_tag="VHS06_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX2987557.1"
                     /translation="MHRPAFRPREGWISLLLLMVVVLSPALSLASADWVDDLWIVPRL
                     ALAALLMGFLLSKLPVRGPVAHWCALEIGVLVVGGFFALHAPATDWESRTIWLGERVW
                     AWIQVAFGGGMSNDSMLFALLMALTGWFLGYLAAWLAFRRHHVWLTLVACGGALLVNL
                     SYGAPDPWVYFGAFLLASLLLLIRMTLHQKESDWVIADAQYGKSLPRVSLLGSSALSV
                     GLLAFAWALPIGTVNGDVAENWYKITGPWQGLQVEFDRLFAAIGTSTPRGEGNRFGKA
                     LALKGAIELGPDLVMLVSSPMPEYWAAQSYDRYTGQGWMSSADQSTRLDANDERLVTT
                     SVYKGRKDIEQRYKLMVGRMSSVFAANAAVKLSLPVYEDHFDTLEEMSALRSTIPMRQ
                     GQQYAVISSVSVASEEQLRDAGTEYPEWTKRYLELPSPAPRRVVSMARRLVRDASDPY
                     DAAVSIQQFLRRFRYEINVATPPTDRDAVDWFLFTAKEGYCDYFASAMAVMARSVGIP
                     ARVVAGYNTGTMNQQTGLYEVKQENAHSWPELFFPNYGWVRFEPTPSQPATERPETPV
                     EEAQTGPTDMEETNLEALSLDTSRRDALLYDDQFDLESGALPADSNSRGNVVQSTIEW
                     SRVVIPAGVVGLLMLAWWGWRRALARLSPSSRAYFQLCSVAALLGWRHSPSNTPAEYA
                     RVLADAAPSFGRELRTVVGCYEEETYGGRRPVGEQRAELSWIRMRRGLSLGLLKVAVK
                     RRLGRN"
     gene            complement(5272..6462)
                     /locus_tag="VHS06_05245"
     CDS             complement(5272..6462)
                     /locus_tag="VHS06_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2987558.1"
                     /translation="MKGLLLIGLTFLVLATALSTGWDLLYRLLYTMAAALAVSFVWAW
                     VNVRWLRFGHEIKNTRAQVGGSIEELLTLENSSWLPKLWLQIVDQSTLIGRRGNRVLS
                     LPGMARRRVSLVTPCTARGMYTLGPADVISGDPFGLFQRRRRLEIGGTVIVYPAITPL
                     NSFGHLPGELPGGSVQSQRTHFVTPNASGVREYQSGDALSRIHWLSSARQNRLMVKEF
                     ELDPLSDVWLILDLDTEVQIGTGQDSTEEVTVAIAATLANYFLREQREVGIIAQGEVL
                     PADRGHRQLHKALDLLAVIRSNSKIPLEQLVLSEETRFARGATAVIVTPSTDEKWLAA
                     CRLLAARGVTVFAVLLEVSTFGDKRSSVPLVSSLAASGVPTYLVKRGDDLALALSSPV
                     RATR"
     gene            complement(6459..>7208)
                     /locus_tag="VHS06_05250"
     CDS             complement(6459..>7208)
                     /locus_tag="VHS06_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2987559.1"
                     /translation="DLLPSDVTGVSIFNQKTAEFEFRPGPVLSQIVLADEINRATPKT
                     QAALLEAMEERQVTVDGVSYRMPRPFVVLATQNPIEYEGTFPLPEAQLDRFFMKIRLG
                     YPGRADELEILGRQERGHPVDQISPVAGEEQLKSAQTLVREVGMAPALRGYIVDIVAA
                     TRTHGDVYLGASPRGSLALFRASQARAMLSGRDYVIPDDVKALAEPVLAHRLIISPSA
                     RIRDVDASLVIREVLDSLPVPGNRIAVSVKR"
BASE COUNT         1490 a         2335 c         2132 g         1251 t
ORIGIN      
        1 catcaagtcc actgaggtaa tgatcggcaa gtagtcggtc cccacaacta cagcgcgtcc
       61 gatgcgcgca atacgagccc ctcccgtggt cacagttggc ctgccacggc gggggctcgt
      121 attgcgccgg tcggccctcg ccgttcagcg gcggaaagtc agcttcgaaa tgcaatggga
      181 atcgactgtc acccgtcccc ggtgccgcgc acctcgacta cagatcacag ttaggttcac
      241 caccccgggc cgctaccccg agccaaggtg gtataatcct cacgggagtc caggacaggg
      301 atctcctccc cgccttgcca tcagccatta tgagccgact gcggcgctgc cagtctcggt
      361 tcgtcgttag gagaagccat ggaagaacaa ctctacaccc aggtcatcga gctcgagggc
      421 cacataatcg actccatgat gctgccccag atcatggacg aggtgatgga ccggggcggc
      481 gatttcgaag tgcaggagat ccgagtcgga cggaagaagt tcgagaccag ctacgctcgg
      541 ttggaaattc gggccaactc cgccgcgctg ctggaccgac tcgtggcgaa cgcccgccgc
      601 atcggcgcca ccgtcccggc ggtggagcag gttcaggctg agcccgcacc cgccgacggc
      661 gtttttccag agggcttcta ctccacgacg aatctggaga cccaggtccg gctgaacagc
      721 gaatgggtca ccgtcgccaa ccccgagatg gacctgggca tcgtggtgga cttcgaggca
      781 ggcacagccc gcaacgtccc aatcgccgac gtgcgcgaag gggaattggt ggtagtcggg
      841 caccaggggg ttcgggtgat accgctggca aggagccgcg aagagaagga aacgttctcc
      901 ttcatgggaa gcgccgtttc cagcgaaaag ccgaaggcga acctgatcag agagatcgcg
      961 acagagatga aggcgatcaa ggcgcgcggt gggaagacgc tggtggtgtc cggcccggcc
     1021 gtcactcaca ccggtgcagc gccgctgatg gtgaggctga tcgaggcggg ctacgtgaac
     1081 tatctgttcg ccggcaatgc cctcgcgacc cacgacatcg agggggcctt cttcgggacc
     1141 tctctcggga tttccctctc ccagggaacg cccatggagg gcggacacca gcaccatctg
     1201 cgagcgatca accgaatacg acgcgcaggg agtatcaagg cggccgtgga gcagggaatg
     1261 ctgaccaggg ggatcatgca cgcttgcgtg aagaacggtg tcgacttcgt gctggcaggg
     1321 tccatccgcg acgacggtcc gctgcccgac gtgataaccg acgtgatcca ggcccagcga
     1381 aggatgcggt cggtgatcca acagggggtg gatctggtgt tgatgatttc caccatgctc
     1441 cactccatag ccgtaggcaa cctgttgccc gcgacggtga caacggtctg cgtggacatc
     1501 aaccccgccg tcgtgacgaa gctggccgac cgggggagct tccaggctat agggctcgta
     1561 gccgacgccg aagggttcct gcggcacctg acaacagagc tggcgatata ggcaggataa
     1621 ccaccgtacg atctctcttt gcctctcttc gatgccgcta ctggccagac ctgacgcggt
     1681 cgaagcaggt gctgcagtac accggcttgt cgcctctcgg caggaagggg acgagggtct
     1741 gggcgccgca atctgcacaa acggcaggat gcatctcacg ggcaggccga gcatccattc
     1801 ctcggccatc ctgtccctgc cgctgagcct tgcgcataga ccgacactct gggcaacggg
     1861 caggctcgtt cagcaagccc ttgagctggt agaactcctg ttcccctgcg gtgaacacga
     1921 actctcgccc acactcacga caccgaagag acttgtccac aaagctcacc ccctcttacc
     1981 tccttttacc tctttgtcgg acggtcctgc aggcacgagc tgttgcagac attcctcgtc
     2041 ggactaccac agtcggattg tggaccacgc agagctcagc cgggggtaat ggataccgcc
     2101 ggtccggaat atacaacaac gttgttgctg attcaatatc tagagcagca attaggttga
     2161 tgaaccgacg aatctggtac caatttctgc gtgaagaagg ggaatccggc actcagatgg
     2221 gcaagaaagg agatcgggca gctattccga atcgtcgaag aagggacact cgtggtgatc
     2281 cggggtgctg cacacctccg atacgtagct actatgaagc tggatgcctg gccggcgctg
     2341 gcaacggaag atgggtcgcc cctcgggatc gccctttgcc tgactgttgt cgcggacact
     2401 ctggaggaag tcacaatact tcgcggccct gtcgcgcccg cccgccatcg gcttcacaga
     2461 acccttgtcg ggcaggggga cgtacacaga tacggatccg tcgaacttgg aggaggcaat
     2521 cgagcactcg gagcagtcca ggtcgccgtg gcaacagggc ttgcgaccgg ggggggaagt
     2581 actcggctgc ccagagatcc cagcagttgc ggcggacgaa atgggcagag cagcgcttcc
     2641 tgcgctcctc gctgcatgac ggctcgatgt cccaacagtg ggttcgtttc tcctcccact
     2701 tctgctcgct gaccgcctcg ctgagagccg ccagcctccc aggtcgacga tcgagtcgtc
     2761 gccgctccgg cctaattatc ttctcgggca ttggtcacct cctgtcggtt gccagctcca
     2821 accgccgcct ttcagttccg ccccaacctg cgcttcaccg ctaccttcaa cagccccaga
     2881 gacagtcccc tgcgcatcct gatccaggac aactccgctc gctgctcccc cacgggacgt
     2941 cgaccgccat aggtctcctc ttcgtagcac cccaccaccg ttcggagctc gcggccgaat
     3001 gaaggtgcag cgtcggccag cactcgagcg tactcggcgg gggtgttgga cggactgtgt
     3061 ctccagccca gcaaagctgc cacagaacag agctggaagt aggctctgct ggagggactc
     3121 aggcgcgcca gtgcccggcg ccatccccac catgccagca tcaacagccc gaccacaccc
     3181 gctgggatca ccacacggga ccactcgata gtcgattgaa cgacattacc cctactatta
     3241 ctatcggcag gtagagcccc gctctctaga tcgaattgat catcgtacag aagagcgtct
     3301 cggcgagaag tatccaatga cagcgcctcg agattggttt cttccatgtc ggtaggacct
     3361 gtctgcgcct cctccaccgg ggtttccggc cgttccgtcg caggctgcga tggggtcggc
     3421 tcgaaacgaa cccatccgta gttggggaag aacagctccg gccaggagtg agcgttctcc
     3481 tgcttgacct cgtacagtcc ggtctgctgg ttcatcgttc cggtgttgta ccccgccaca
     3541 acccttgcgg ggatgccgac cgaccgcgcc atcaccgcca ttgcggaggc gaagtagtcg
     3601 caatagccct ccttggcagt gaataggaac cagtcgaccg catcgcgatc ggttggcggc
     3661 gtggcgacat tgatctcgta acgaaaacgg cggaggaact gctgtatgct caccgccgca
     3721 tcgtatggat ccgatgcatc ccggaccagc cgtctggcca tagacaccac ccgacgcggc
     3781 gcgggggagg gcaactccag gtagcgcttg gtccactcgg ggtactccgt ccccgcatct
     3841 cggagttgtt cctcgctcgc caccgaaacg gaggagatca cggcgtactg ctgcccctgg
     3901 cgcatcggta tggtgctcct gagggcagac atctcctcca gggtgtcgaa atggtcctca
     3961 tacaccggca ggctgagctt cacggcggcg ttggccgcaa agacggagct catccggccg
     4021 accatcagct tgtaacgttg ctcgatgtcc ttgcgcccct tgtagacgct tgtggtcacg
     4081 agacgctcat cgttggcatc caacctggtg ctctgatcgg ccgagctcat ccagccctgg
     4141 ccggtgtagc ggtcgtaact ctgcgccgcc cagtactcgg gcatcgggga cgagaccagc
     4201 atcaccagat ctggccccaa ctcgatggct cccttgagcg ccagtgcctt gccgaatcgg
     4261 ttcccctcac cgcgcggagt ggacgtgccg atggcggcaa agagtcgatc gaactccacc
     4321 tggagcccct gccacggtcc cgtgatcttg taccagttct cggccacgtc gccgttgaca
     4381 gtgccgatag gcagagccca ggcgaaggca agcagtccca cagaaagggc ggaactgccc
     4441 agcagcgaga cccgaggcag cgacttgccg tactgggcat cggcgatcac ccaatcgctt
     4501 tccttttggt gcagcgtcat cctgatcaag agcagcaagg aagccagcaa gaaggcaccg
     4561 aagtagaccc aggggtcggg ggcaccgtag gacaggttca ccagcagagc gccgccgcac
     4621 gcaaccagtg tcaaccagac gtggtgtcgc ctgaaggcga gccacgcggc caggtaaccc
     4681 aggaaccacc ctgtcagcgc catcagcagg gcgaacaaca tgctgtcatt gctcatgccg
     4741 ccgccgaatg ccacctggat ccaggcccac acccgctcgc caagccagat agtccgagac
     4801 tcccagtccg tcgcgggcgc gtgcagggca aaaaagcctc ccaccaccag cactccgatc
     4861 tccagagcac accagtgggc gacgggccct cgcaccggca acttgctcag caggaacccc
     4921 atgagcaacg ccgccagggc aagtctggga acgatccaca ggtcgtccac ccaatccgcg
     4981 ctcgccaggg agagcgcggg gctgagaacg acgaccatca gcagcaggag cgagatccac
     5041 ccctcccgcg ggcggaaggc aggtcgatgc atgagcaacc agctcctatc ttctacaacg
     5101 aggattgggc ttccccgtta caaaacaaca gcacgtctga ggcaggggcg acccgcccta
     5161 ctggaagagt cgcggtgcca cagcagggaa tatgcaactc caaggatttg gcccgctggt
     5221 acaatcctcc tcagcactca gaacccagaa cccagaaatc agaacccaga actacctggt
     5281 ggccctcacc ggacttgaga gtgccagcgc gagatcgtcg ccccgcttca ccaggtaggt
     5341 tggcacgcct gaagccgcca gcgagctgac cagcggaacg ctgctccgtt tgtccccaaa
     5401 ggtgctcacc tccagcagca ccgcgaacac cgtcacacca cgcgccgcca acagccggca
     5461 cgccgccagc cacttctcgt cggtggaagg ggtcacgatc accgcggtcg ccccgcgggc
     5521 aaagcgagtc tcctccgaca gaaccaactg ctccagcggg atcttgctgt tggagcggat
     5581 cacggcaagc aaatcgagcg ctttgtgaag ctgccgatgg ccacggtcgg ccggcagcac
     5641 ctccccttga gcgataatgc ctacctccct ctgctccctc agaaagtagt tggccagggt
     5701 ggcagctatc gcaaccgtaa cctcctccgt agagtcctgg ccggttccaa tctgcacctc
     5761 tgtgtccaga tccagaatga gccagacgtc cgacagcgga tccaactcga attccttcac
     5821 catcagccgg ttctgtctgg cggaactgag ccagtggatc cgactgaggg catcgcccga
     5881 ctgatattcc cggacacctg aagcattggg ggtcacgaag tgggtgcgct ggctctgaac
     5941 gctgccgccc ggcagttcac ccggcaggtg cccgaaagag ttgagggggg tgatggcagg
     6001 atagacgatg actgtgccgc ctatttccag ccgcctgcgc cgctggaata ggccaaaggg
     6061 atcgccgctg atcacgtcag cagggccgag ggtgtacata ccgcgagccg tgcacggcgt
     6121 aaccaacgac actcgccgcc tcgccatgcc tggcaaggag agtactcggt ttccccgacg
     6181 gccgatgagc gtagattggt cgactatctg gagccacagc ttcgggagcc aactactgtt
     6241 ctccagcgtc agcaactcct cgatgcttcc cccaacctgt gctctcgtgt tcttgatttc
     6301 gtgaccgaac cgaagccagc gcacattgac ccaggcccac acgaaggaga cggctaacgc
     6361 ggcggccatc gtgtagagca accgatacag tagatcccac ccggtgctga gtgcggtagc
     6421 cagcaccaag aaggtgagac ctatcagaag taagcctttc accgtttgac cgagaccgct
     6481 atccgattgc cgggaaccgg cagggagtcc agcacctccc gaataaccag agatgcgtct
     6541 acatccctta tgcgagccga cgggctgatg atcaacctgt gagccaggac cggctccgcc
     6601 aacgccttca cgtcgtccgg aatgacgtag tcgcgaccgg agagcatcgc tctagcctga
     6661 gacgcacgaa agagggcgag agagcctcga ggactggcac caaggtagac atctccatgg
     6721 gtacgggttg cggcgacgat atccacgatg tagcccctga gagcaggagc catacccacc
     6781 tcccggacca acgtctgagc agacttcagc tgctcctccc cggcaaccgg ggagatctga
     6841 tccacaggat gcccccgctc ctgccggcca aggatctcca gctcatcggc tcgaccggga
     6901 taacccaatc tgatcttcat gaagaagcga tccagttgag cctccggcag cgggaaggtg
     6961 ccctcgtatt cgatgggatt ctgggtagcc agcaccacaa agggtcgggg catcctgtag
     7021 gagaccccgt ccaccgttac ctggcgctcc tccatcgcct ccaacaacgc ggcctgggtc
     7081 ttaggcgtag cccgattgat ctcgtcggcc aggacgatct gtgaaaggac cgggcccggc
     7141 cgaaactcga actcggcggt cttctggttg aagatcgaca cgcccgtcac gtcgcttggc
     7201 agcaggtc
//
LOCUS       DATALS010000235         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_206255, whole genome shotgun sequence.
ACCESSION   DATALS010000235 DATALS010000000
VERSION     DATALS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206255"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VHS06_05255"
     CDS             <1..417
                     /locus_tag="VHS06_05255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2987560.1"
                     /translation="LSAALLLASLGRTPRRRAYLGSAAALGILQMLLLVVHQYLPALF
                     ILVGLGFSMIFFTTLSNTVLQTSTPDMLRGRVMSVYTTVFVGSTPLGSLFAGGIAENW
                     GVGAAFFACGVAALGMALVGIPLSRGSAKTVAPIRP"
     gene            complement(404..1288)
                     /locus_tag="VHS06_05260"
     CDS             complement(404..1288)
                     /locus_tag="VHS06_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2987561.1"
                     /translation="MIGEISGLACAAGWAVISTTMKAVSNGVSPVMVNAVRCAFSTLV
                     LAAIVVATGQIGTLSTLPLSAVAAIVASGVLGQAIGDALFISSMKLIGASRALPISGI
                     NPILTMVLAMLALSEQVSLLSAVGTFLVVGGVYFLAFPYGTPKQASHGAGSGDRVGIL
                     MTLAATVAWATSTVVLKAGIQDVNLLVANLVRISGATLFLFSIELFHSRGRVAIGLTP
                     RSIRVLALAGGLNGITGILYLVSLTYTGAAKAAVLSATSPVFALPISVFFLKERINRR
                     ILIGTGLCIVGIWMVMGG"
     gene            1479..1871
                     /locus_tag="VHS06_05265"
     CDS             1479..1871
                     /locus_tag="VHS06_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX2987562.1"
                     /translation="MRYDQIVGQVQNKARLASEGEALRAIQATLETLGERVYGGEAHN
                     LASQLPRELGQFLEEPAINESFDMDEFFRRVSLREGVDLPEAVFHARAVIDVLRQAVS
                     PGAIDKLLDQLPEEYDRLFVSGSTGRMP"
     gene            complement(1983..>2572)
                     /locus_tag="VHS06_05270"
     CDS             complement(1983..>2572)
                     /locus_tag="VHS06_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dynamin"
                     /protein_id="HEX2987563.1"
                     /translation="AEELARRRKASERSTPTSGFGGEFEESRRELIQNVARTARVVIA
                     GFDRESEARKLGDAMREAVAHTALAEVGALGLGTLVAVLIGTAAADVSGILAGTLIAG
                     LGLYIIPARKQRVQKQFRQRTDELRITLRDALAKQLKVELNSSLERIETAITPYLRFV
                     RSEQEKLSAFHDRLETLGDEIGALRRRVGTPPIES"
BASE COUNT          457 a          802 c          839 g          474 t
ORIGIN      
        1 ctttcggcgg cgctgctgtt ggcgtcgctg ggccggaccc cgcgtcgccg ggcctacctg
       61 ggcagcgctg ccgcgctggg gatcctccag atgctgttgc tggttgtgca ccagtacctg
      121 ccggcgctgt tcatactggt cgggctcggc ttctccatga tcttcttcac gaccctctcc
      181 aacacggtgc tccagacttc cacgccggat atgttgcgag ggcgtgtgat gagcgtttac
      241 accacggtct tcgtgggctc cacgccgctg ggcagcctct ttgccggtgg catcgccgag
      301 aattggggcg tgggggcggc cttcttcgcc tgcggcgtcg ccgccctggg gatggcgctg
      361 gtggggatcc cgctcagccg ggggagtgcg aagacggtag cccctatccg cccataacca
      421 tccatatccc cactatgcac agccccgtcc cgatcaggat tcggcggttg atccgctcct
      481 tgagaaagaa gacggagatg gggagggcga atacggggga ggtggcagag aggaccgcgg
      541 ctttcgccgc gccggtatag gtgagggaga cgaggtacag aattcccgtg atgccgttga
      601 ggccgccggc aagggccagg acgcggatcg atcggggtgt aagcccgatc gcaaccctgc
      661 cgcgagagtg gaacaattcg atgctgaaga ggaaaagcgt agcaccgctt atcctgacca
      721 ggttcgcgac caagaggttg acgtcctgga tgccggcctt caggaccacg gtggaggtgg
      781 cccacgcaac cgtggcggcc agcgtcatca ggatccccac acggtcaccg ctccctgccc
      841 cgtggctcgc ctgcttcggt gtcccatatg ggaaggccag aaagtagact ccacccacca
      901 ccaggaatgt cccgacggcg ctgagcaggc taacctgctc gctcagggcg agcatggcca
      961 gaaccatggt gaggatcggg ttgatgccgg agatgggcag tgccctggag gcgccgatta
     1021 gcttcatgct gctaatgaat agagcgtcgc cgatcgcttg ccccagcaca cccgatgcga
     1081 cgattgcggc cactgctgat agtggcaggg tggagagggt gcctatctgc cctgtggcga
     1141 cgacgatggc ggccagcacc agggtggaga aggcgcagcg cacggcgttc accatcacag
     1201 ggctgacgcc attggaaact gccttcatgg tggtgctgat aacggcccag ccggccgcgc
     1261 aggcgaggcc ggagatctct ccgatcaatt cgttgtgagc tcccgaaatg gtctctcgta
     1321 ggtgtcaaac cgtggaaaaa cgaggagcac tatcatagca gggacccttt gcctggcgcg
     1381 acccttgcgg acgtggcggc cctgcacccg gagcagtcgg ttccaactga agtggggctg
     1441 ccggttccgg tgtcatgcgt ggacgagagg aaagggagat gagatacgac cagatagttg
     1501 gacaggttca gaataaggct cgcctggcat ctgaggggga ggcgctgcgg gccatccagg
     1561 caaccctgga gactcttggc gagcgggtat acggcggcga ggctcacaac ctggcatccc
     1621 aactccctcg cgagttgggg caattcctgg aggagccggc gatcaacgaa agcttcgaca
     1681 tggatgagtt cttccgccgc gtcagcctgc gcgagggcgt ggacctacct gaggcagtct
     1741 tccacgccag ggcggttatc gatgtgcttc gccaggcagt ctcaccgggg gccatcgata
     1801 aactgctgga ccagcttccg gaggagtacg accgactctt cgtgtcgggg agcaccggcc
     1861 gaatgccgta aatggagcac gggggcgcga tctcgcgccc ccgtgccata actgagcggc
     1921 tttccctaga tgccgtcaac ctttccatga ggggaaagtg gacaccaaca agggaaagtc
     1981 gcttaactct cgatgggagg agtgcctacg cggcgccgta gcgccccgat ctcgtcaccg
     2041 agcgtttcca gacggtcgtg gaaggcgctc agcttttcct gctctgagcg cacgaagcgc
     2101 aggtaagggg taatggccgt ctcgatccgc tccagtgagg agttcagctc cacctttagc
     2161 tgtttggcca gtgcgtcccg aagggttatc ctcagttcgt cggtgcgctg ccggaactgc
     2221 ttctgtaccc gctgctttcg cgcggggatg atgtacagtc ccagccccgc gatcagggtg
     2281 ccggccagga tccccgacac gtccgccgcg gcggtaccta tcagcaccgc gaccagcgtc
     2341 ccgagaccaa gcgcgcccac ctcggccagc gccgtgtgcg ccaccgcttc tcgcatggcg
     2401 tctcccagct tccttgcctc agattcgcga tcgaagccgg ctatcaccac tctggcggtg
     2461 cgggccacgt tctggatcaa ctccctgcgg ctctcctcga actccccgcc gaacccactc
     2521 gtcggcgtgc ttcgctcgga cgcttttcgg cgacgggcca gctcctctgc cg
//
LOCUS       DATALS010000236        10562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_381182, whole genome shotgun sequence.
ACCESSION   DATALS010000236 DATALS010000000
VERSION     DATALS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10562
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381182"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..101
                     /locus_tag="VHS06_05275"
     tRNA            25..101
                     /locus_tag="VHS06_05275"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Trp,seq:cca)
     gene            163..384
                     /gene="secE"
                     /locus_tag="VHS06_05280"
     CDS             163..384
                     /gene="secE"
                     /locus_tag="VHS06_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX2987564.1"
                     /translation="MPVKEKDKKGRSAVDRTKDFIREVRTELRKVVWPTRQEAINLTA
                     IVIGVSVAVGVFLGFVDFVFAQGFQLLLR"
     gene            411..1013
                     /gene="nusG"
                     /locus_tag="VHS06_05285"
     CDS             411..1013
                     /gene="nusG"
                     /locus_tag="VHS06_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX2987565.1"
                     /translation="MEQEEAQAQVVEQEALEQQEAAERAHPDAQWFVIHTYSGYENKV
                     KTNLEHRVDSMGAQDQIFQVVIPTEDEIEIRDGQRRTVQKKVFPGYVLVLMLLTDHSW
                     HVVRNTPGVTGFVGSGSKPTPLMDQEVKQILKAMKREAPKIKVSFSRGERVKVVDGPF
                     TDFIGVVDDINLEKGKVRVLVSFFGRETPVELDFLQVSRV"
     gene            1092..1517
                     /gene="rplK"
                     /locus_tag="VHS06_05290"
     CDS             1092..1517
                     /gene="rplK"
                     /locus_tag="VHS06_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX2987566.1"
                     /translation="MAKKVKAIVKLQISAGKATPAPPVGPALGQHGVNIMAFCKEYNE
                     RTASQAGLVIPAEITIFEDRSFSFITKTPPTVDLIKRAISLDKGSSVPNKEKVGTLSA
                     AKVREIAEAKLKDLNAIDLDGAIKMVEGTARSMGVTVSE"
     gene            1575..2285
                     /gene="rplA"
                     /locus_tag="VHS06_05295"
     CDS             1575..2285
                     /gene="rplA"
                     /locus_tag="VHS06_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX2987567.1"
                     /translation="MPRHGKQYVEAAKKVEEDKLYTPAEAVKKLREVSFTKFDPTIEV
                     HIRTGVDPRHADQMVRGLAVLPAGTGKPVRVLAFAQGEKAKEAQDAGADFVGAQDMVA
                     KIEGGWLDFDTAVATPDMMGMVGKLGKILGRRGLMPNPKAGTISFDISKVVKEVKAGR
                     VEFRVDKTAVIHVPIGKLSFSDEQILTNLGSLIDAVVRAKPSGAKGQYVQTIALASTM
                     SPSIHLDLGPTLSMASGS"
     gene            2494..3051
                     /gene="rplJ"
                     /locus_tag="VHS06_05300"
     CDS             2494..3051
                     /gene="rplJ"
                     /locus_tag="VHS06_05300"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX2987568.1"
                     /translation="MPKGKKGQIVREKKAREVDELTGLLSRSNLVVLADYRGLSVTDM
                     AGLRRKLRGQGIELKVAKNTLTTFAAEKAGKGDLKTALTGPTAIAFVTADEAAGAKTL
                     SDFEHSSKVFKIKGGLLGAKALTAGEIGHLATMPPREVLLAQVVAGFQAPIAGMVGVL
                     GGVLSGFVGTLEARRRQLEEQGAAS"
     gene            3184..3567
                     /gene="rplL"
                     /locus_tag="VHS06_05305"
     CDS             3184..3567
                     /gene="rplL"
                     /locus_tag="VHS06_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX2987569.1"
                     /translation="MSTEEILSAIEKMTVLELADLVKAMQDRFGVVAAAPVAAAAAPG
                     AAAAAAPAAAAEEQTEFNVVLTNFGANKINVIKVVRELTGLGLKEAKDLVEGAPKPVK
                     EGVAKDEADKAAAKLKEAGATVEVK"
     gene            complement(3622..4488)
                     /locus_tag="VHS06_05310"
     CDS             complement(3622..4488)
                     /locus_tag="VHS06_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEX2987570.1"
                     /translation="MKQVTILGLGVMGTSLGLALKQLKSPPRVVGCDVQYDAAQGATR
                     LKAVDQIERDPLVAVRGSELVILAAPVGAIQELIQTIAPSLEQGSVVTDLGSTKREIV
                     RKGDELLSPNAAFVGGHPMTGPTTAGVDGPSASIYSGAIYCLTPTANTPPDAVQMVAN
                     MVHEIGAEAYFLDPSEHDGLVAAISHLPYLVSVAVMRCLAGQPGWREMSTLAAGGFDT
                     ATRLSGSGATMFADVLTTNADNVARQLDRLVEELQSFKLKLLNCDPGIERDLEEAQSL
                     RRQWEEERRPKQ"
     gene            complement(4501..5034)
                     /locus_tag="VHS06_05315"
     CDS             complement(4501..5034)
                     /locus_tag="VHS06_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX2987571.1"
                     /translation="MRPLYRVRQGLDNLWAGVGSRDLAIVRQQLSQEEFQLFLRMDSA
                     DQRHCIRVLRAIQAEGNCDANLTKAALLHDVGKARCHISIVHRTIAVLIGGVFRRLPP
                     LAFAPSSGSWWYPFYVIANHPRIGAAMLVRCGSPERVWRLAELHHLEPHQVGRLQKDN
                     EWVQRALLALRRADNQN"
     gene            complement(5038..5655)
                     /locus_tag="VHS06_05320"
     CDS             complement(5038..5655)
                     /locus_tag="VHS06_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2987572.1"
                     /translation="MDLTLDSLLLFVPAFVIAIAMHEASHAFAATILGDPTPRVQGRL
                     SLNPLRHLDPLGTITLFLVGFGWGKPVVVNPRYFKVNPLAGMALTAVAGPLMNVLLAL
                     LFALPFRMQWHMVGWPLAFAEAVASINLLLAVFNLIPLPPLDGFSVLMGILPRPLAIK
                     IAPLAQYGPGALLAIFALGYVFKLNILGAIMAPPYHLLQPLIFGG"
     gene            complement(5717..7018)
                     /locus_tag="VHS06_05325"
     CDS             complement(5717..7018)
                     /locus_tag="VHS06_05325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2987573.1"
                     /translation="MERRRTAEDAIRWEIEQSGPITFRRFMELALYGGPTGYYSSGTE
                     RVGRSGDFLTSPEVHPAFGSLLARQLEQLWRAMGRPDPFPVVEMGAGRGSLALDILSY
                     CAQWSPPLHNALAYTILEPNPTLADRQRELLASSSEAAAKVRWRITDSLDLPERSLEG
                     CVVTNELLDAFPVHRVRNQSGALQELYVGVEGHRLVDVPGKPSDPALEEYFDRLGLLP
                     PAGCKAEVNLDALQWMRRVASVLDRGAAITIDYGYPATDLYSRRHCDGTLLCFYRHTL
                     NADPYARIGEQDITTHLDFTSLMAEAERLGLQQAGLTTQREFLRALGIDGYRRRLSAL
                     GLRRRDLDANGLAMDALVAPQGLGRIGVMIQVKGVDEFFPAGLRPDGFRPAELGRDFT
                     LETPPLLRSQQLRLNASPTAELSQAETEDLWRDLMAEDDML"
     gene            complement(7255..8364)
                     /locus_tag="VHS06_05330"
     CDS             complement(7255..8364)
                     /locus_tag="VHS06_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX2987574.1"
                     /translation="MSIGYAFSTKDTVSAAVEELATKLAGVGPKAVLYFASPVYDPHT
                     LSAAVQTAFGDAVTIGCTTAGEIVSGHMLKHSIVAMAFGADCVEDIAVEVVERVREEN
                     RVPAALANLGKKFGTPMRRMEFEKYVGIILVDGLSGSEERLMDTIGDLTDVRFIGGSA
                     GDDVRFEATYVFVNGRALSDAAVLALIKPSARFDIIKTQSFVPTAKQLVATKVDTATR
                     TVLEFDNMPATKAYAKAVGVEVKDVANSFMQHPLGLMAGDEPFVRSPQQVKGDSIVFY
                     CNVLEGMSLSILDSTDLIADTRKAIEDKEKEFGKIAALLNFNCILRTLELEALGRTED
                     YGRLFSTIPTVGFSTYGEQLFGHINQTATMLVFGK"
     gene            complement(8379..8588)
                     /locus_tag="VHS06_05335"
     CDS             complement(8379..8588)
                     /locus_tag="VHS06_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987575.1"
                     /translation="MAGKIKVMIDRIIQERSKGNPTLASVTTTKLILKGINPASYGPT
                     SADDPAVMQKLESLARELGLSNIRL"
     gene            9016..9474
                     /locus_tag="VHS06_05340"
     CDS             9016..9474
                     /locus_tag="VHS06_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX2987576.1"
                     /translation="MDDQLVVFRLATDHYGVGISAVESIIKMQPITEVPLAQQFVEGV
                     INLRGKVVPVIDLRKRFGLGCEQVGKDTRVIVVNAGGVTIGMVVDEVSEVLRVDQGAV
                     EPPSPVVATAESAFVRGIAKVDERLIILLDLGRILTEEQRADLEQIPAAV"
     gene            9789..>10562
                     /locus_tag="VHS06_05345"
     CDS             9789..>10562
                     /locus_tag="VHS06_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX2987577.1"
                     /translation="MPDFQLASDFVPTGDQPQAIEQLVRGLQSGMHCQTLLGVTGSGK
                     TFTMANVIAQMQRPTLVMAHNKTLAAQLCSEFKEFFPENSVEYFVSYYDYYQPEAYVP
                     KSDLYIEKDSSLNEEIDRLRHAATKALFERRDVVIVASVSCIFGLGSPEDYGQTVLKI
                     KKGEVRKRDKVLRFLTDIHYERNDYNFARGKYRVRGDTLEIYPVGEDLAIRIEFWGDE
                     VERITEVDSLTGEVLVERTQVDVYPAKHFVTTQEKLEAAL"
BASE COUNT         2071 a         2952 c         3415 g         2124 t
ORIGIN      
        1 aagagatagt aagcaataca gtgtaggcct gtagctcaat taggcagagc agcggtctcc
       61 aaaaccgcag gttgtcggtt cgattccggc cgggcctgcc aaactgtctc taggacagct
      121 ttaacagctg gtttaactcc aggccaatcg agtaggagaa caatgcccgt caaggaaaaa
      181 gacaagaagg gcaggagtgc ggtcgaccga acgaaggatt tcattcgtga ggtgcgcacc
      241 gagctgcgca aggtggtatg gcccacccgc caggaggcga tcaacttgac ggcgatcgtg
      301 atcggcgtgt ccgtggccgt cggggtgttc cttgggtttg ttgactttgt cttcgcccag
      361 ggatttcaac tgctgttgcg gtaggcagcg aatccgtatt gagtggctta atggaacagg
      421 aagaagcgca agcccaggta gttgagcagg aagccctgga gcagcaggaa gcggcagagc
      481 gggctcatcc tgatgctcag tggttcgtta tccacaccta ttcgggctat gagaacaagg
      541 tgaagaccaa cctggagcac cgggtcgatt ccatgggtgc tcaggaccag atctttcagg
      601 tggtgattcc caccgaggac gagatcgaga tccgcgacgg gcagcggcgt accgtgcaga
      661 agaaggtatt ccctgggtac gtgctggtgc tgatgctgct gaccgatcat tcgtggcacg
      721 tcgtgcggaa tacgcctggc gttaccggtt tcgtcggatc tggtagcaag cccacgccgc
      781 tgatggatca agaggtcaag cagatcctga aggcgatgaa gcgtgaggcg ccaaagatca
      841 aggtcagctt ctctcgtggc gagcgggtca aggttgtgga cggaccgttc acagacttca
      901 tcggtgtcgt cgatgacatc aacctggaga agggtaaagt cagggtgttg gtgtccttct
      961 tcggtcgtga gactccggtg gagttggact tccttcaggt tagcagagtc taggcggcga
     1021 aagcggaaac aagcagggca ggcggcgacg actgcggtcg tcgcctgttt gtgagtgtaa
     1081 ggagacttct catggcgaag aaggtcaagg cgatagtaaa gctgcagata agcgccggca
     1141 aggccacccc cgcgccgccg gttgggcctg ccctcggcca gcatggtgtc aacatcatgg
     1201 ccttctgcaa ggagtacaac gagcgtaccg cttcacaggc ggggcttgtt attccggccg
     1261 agatcaccat tttcgaggac cggtcgttca gcttcatcac gaagacccct ccgacggtcg
     1321 acctgatcaa gcgagcgata tccttggaca aggggtcgtc ggttccgaac aaggagaagg
     1381 ttgggacgct gtccgcggcg aaggttcgag agatcgccga ggcgaagttg aaggacctga
     1441 acgctatcga cctggatggg gccatcaaga tggttgaggg caccgccagg agcatgggag
     1501 tgacggtatc cgagtagggc cgtcgatttt gtgggagggt gcgtcgcgcc cgtttgcacc
     1561 acgaggagga aacaatgcca aggcacggaa agcagtatgt tgaggccgcc aagaaggtag
     1621 aggaggacaa gctctacact cccgcggagg ccgtgaagaa gctacgagag gtttctttca
     1681 ccaagttcga ccccaccatt gaggtgcata tcaggaccgg cgtcgatcct cggcacgcgg
     1741 accagatggt cagggggctc gcggtcctac ctgccggaac agggaagccg gttcgggtgc
     1801 tggcgttcgc tcagggtgag aaggcgaagg aggctcagga cgcgggcgcg gacttcgtcg
     1861 gggcgcagga catggttgcc aagatcgagg gtggttggct ggattttgac acggctgttg
     1921 ccacccctga tatgatggga atggtcggta agcttggtaa gatcctgggt cgccgcggcc
     1981 tgatgccgaa ccccaaggcc ggcaccatct ccttcgacat cagcaaggtg gtcaaggaag
     2041 tcaaggctgg tcgggtggag ttcagggtcg acaagactgc cgttatccac gttccgatcg
     2101 ggaagttgag tttctccgat gagcagatcc tgacgaactt gggctcgctg atagacgctg
     2161 tcgtaagggc gaagccgagt ggtgcgaagg gtcagtacgt tcagacgatc gcccttgcct
     2221 ctacgatgag ccccagcatt cacctggatc tgggaccgac gttgtcgatg gcttcgggtt
     2281 cgtagcaggg ttctaaaccg aatatgtggt ctgcgctgta gacagcgggt gtgctgcaaa
     2341 ggcacttaat gtggcctgcc gaggcgagga cacgagatga cgttccggta gtacggtatg
     2401 agaatcttgg gtctttggct cagcagccaa gggccctttt cttttgtgtg cagcgcatca
     2461 ctctcgccat cttggtggaa aggaggtgaa gctttgccaa agggtaagaa gggtcagata
     2521 gtcagggaga agaaggcccg cgaagttgac gagttgaccg gtcttctgtc ccggagcaac
     2581 ctggtggttc tggcggacta tcgaggtcta tcggtgacgg atatggctgg tctgcgccgc
     2641 aagttgcgtg gccagggtat cgaactgaag gtcgcgaaga acacgttgac taccttcgct
     2701 gctgagaagg cggggaaggg tgatctgaag accgctttga cgggcccaac ggcgatcgca
     2761 tttgtgaccg ctgacgaggc agcaggcgcc aagaccctga gcgacttcga gcattcgtcg
     2821 aaggtgttca agatcaaggg cggcctgttg ggtgccaagg cgttgacagc cggcgagatc
     2881 gggcatctgg cgacgatgcc gccgcgcgag gttctgctcg cacaggtggt tgccggtttc
     2941 caggccccca tcgcgggcat ggtgggtgtt ctgggcggag tgctcagcgg ctttgtcgga
     3001 acgctggagg cgcgccggcg acagctggag gagcagggag cagccagcta gccttcacta
     3061 ttgaagctgc tggatttggc ccgggatggc agttccatcc cctgtgggcc gatgagtcgg
     3121 acggcccgag tgccgagccg gaattcgact tcacgtatgc caagtaatag ggaaggaatt
     3181 gagatgagca ctgaagagat cctctcggcc atcgaaaaga tgaccgttct cgagctggct
     3241 gacctggtta aggctatgca ggataggttt ggcgtagtgg ccgcggcccc cgtggctgcc
     3301 gccgccgctc cgggcgctgc cgccgccgct gctcccgctg ctgctgctga ggagcagacc
     3361 gagttcaacg ttgtcctgac caatttcggc gccaacaaga tcaacgtgat caaggtggtc
     3421 cgcgagttga ccggcctggg cctcaaggaa gcgaaagacc tggtcgaggg cgcgccgaag
     3481 cccgtgaagg aaggcgtggc caaggacgag gccgacaagg ctgccgccaa gctgaaggaa
     3541 gccggcgcaa ccgtcgaggt caagtaacta gaaatggcgt aggggcgacg catgcgtcgc
     3601 ccctacaacc ccaactagcc gctactgctt ggggcggcgt tcctcttccc attgtcttcg
     3661 caggctctgc gcttcttcta ggtcccgctc aataccggga tcacagttca gcagcttcag
     3721 cttgaacgac tgcaactcct ccaccaatct gtccaactgg cgggcgacat tgtccgcgtt
     3781 cgttgtcagc acatcggcga acatcgttgc tccgctgcct gaaagtctcg tggcggtgtc
     3841 gaagcctccg gccgcgagcg tggacatctc tctccacccc ggctgaccgg caagacagcg
     3901 catcaccgcg acggacacca ggtagggcag gtggctgatg gcggccacta gaccgtcatg
     3961 ctccgacgga tctaggaagt aggcctccgc gcctatctcg tggaccatat tggcgaccat
     4021 ctggacggca tctggagggg tgttggcagt tggggtgagg cagtagattg cccccgagta
     4081 gatggacgcg cttggtccat ccacgccggc agtggtgggc cccgtcatgg gatggccacc
     4141 gacgaaggct gcgttggggg acagcaattc gtcgcctttg cggacgatct ctcgcttggt
     4201 gctgccgagg tcggtcacga cgctgccctg ttccagcgac ggtgcgatag tctggatcaa
     4261 ttcttgaatc gcgcccaccg gtgccgccag gatcaccagc tccgagcctc tcacggcgac
     4321 gagtgggtct cgctcgattt ggtccacggc cttcagcctc gttgcccctt gtgcggcatc
     4381 gtactgcacg tcgcatccca cgacgcgcgg tggggacttg agttgcttca gggccagccc
     4441 gagagaggtt cccatcacac cgagcccaag gatggtaacc tgcttcatag catctctacc
     4501 tcagttttga ttgtcggcgc ggcgcagagc aagcagcgcc cgctgaaccc attcgttgtc
     4561 cttctgaagc ctgccgacct ggtgcggttc cagatggtgg agctccgcga gcctccagac
     4621 gcgctcgggc gagccgcacc tgacgagcat cgcggcacct attctcggat ggttggcgat
     4681 tacgtagaag ggataccacc aacttcccga ggaaggcgcg aaggcgagcg ggggcagcct
     4741 gcggaacacg ccgccaatga gtaccgcgat ggtgcgatgg acgatgctga tgtgacatcg
     4801 ggcctttccg acgtcgtgga gaagcgcggc cttggtcagg ttggcgtcgc agttgccttc
     4861 agcttggatc gcgcggagga cgcgaatgca gtggcgttga tccgctgagt ccattcgcaa
     4921 gaagagttga aactcctctt gcgagagttg ctgtcggacg atggccaggt ccctcgagcc
     4981 gacaccggcc cagaggttat cgaggccctg gcgcacccga tagagcggac gcactttcta
     5041 cccgccgaag atgagcggct ggagtagatg gtagggcgga gccatgatcg cacccaggat
     5101 gttcagcttg aagacgtagc ccagggcgaa tatggccagc aacgcgcctg gaccgtactg
     5161 ggcgagagga gcgatcttta tggccagtgg cctgggcagt atgcccatca gaacggagaa
     5221 gccgtccaga gggggaagcg ggatcaggtt gaacacggcc agcaggaggt tgatagaggc
     5281 cacagcctca gcgaatgcga gcggccaacc taccatgtgc cactgcatcc tgaacgggag
     5341 tgcaaaaagc agcgccagca gcacgttcat cagcggaccc gcgaccgccg tcaacgccat
     5401 gccggcgagg gggttaacct tgaagtaacg cggattcacc accacgggct tgccccaccc
     5461 aaacccgacg agaaagaggg tgatggtccc gaggggatcc aggtggcgca atgggttcag
     5521 gctcagccga ccctggacgc gcggggtggg gtcaccgaga atcgtagcgg cgaatgcgtg
     5581 gctggcctcg tgcatcgcga tggcgatgac gaatgccggg acgaacagca gcagactgtc
     5641 gagagtgagg tccatctatc ctccgacgaa aggggtcgtg atcgaccgcg accccggcga
     5701 aacggataac acttcattat agcatgtcgt cttcggccat caggtcacgc cacagatcct
     5761 ctgtctccgc ttgtgacagt tcggccgtag gggaggcatt gagccgtagc tgttgagacc
     5821 tcagcagcgg cggggtttcc aacgtgaagt cgcggccgag ttcagcgggc cggaaaccat
     5881 cgggccgcag ccctgcgggg aagaactcgt cgacaccctt gacctggatc atcacgccga
     5941 tgcgcccgag cccttgtggt gcgaccagtg cgtccatcgc caggccgttt gcgtcgaggt
     6001 cgcgccgtcg cagtcccagg gccgagagtc gccggcggta cccgtcgatc cccagcgcgc
     6061 gtaggaactc gcgctgcgtc gtcaggcccg cctgctgaag tcccagtcgc tcagcctcgg
     6121 ccatcaggct cgtgaagtcc aagtgagtcg tgatgtcctg ctctcctatt cgggcgtatg
     6181 ggtcggcgtt gagcgtatgc cgatagaagc agagtaaggt gccgtcacag tgccggcgag
     6241 agtacaggtc cgtggcggga tagccgtagt cgatcgtgat cgccgcacct ctatccagca
     6301 ccgacgccac ccgtcgcatc cattgcaggg cgtccaggtt cacttcggcc ttgcagcccg
     6361 cgggcgggag cagtcccaga cggtcaaagt actcctcaag tgcagggtca gagggcttgc
     6421 ccgggacgtc taccagcctg tgtccctcga ccccgacata cagctcctgc agagcccctg
     6481 actggtttct gactctgtgc acggggaagg catccaggag ttcgttcgtg acgacacagc
     6541 cctccaggga ccgctcgggg aggtcgaggg agtcggtaat ccgccaccgc accttggctg
     6601 ccgcctcaga tgacgaggcg agcagctcac gctgtcgatc ggcaagcgtc gggttgggct
     6661 ccaggatggt gtatgcaagg gcattgtgga gtgggggact ccactgtgcg cagtaggaaa
     6721 ggatatcgag ggcaagtgag cctcggcccg cgcccatctc gacgacgggg aaagggtcgg
     6781 gtcgtcccat ggcccgccag agttgctcca actgcctggc gaggagtgac ccaaaagcag
     6841 ggtgtacttc cggactggtc aagaagtctc cacttcttcc gactcgttcc gttcctgagc
     6901 tgtagtagcc tgtagggccg ccgtagaggg ccaattccat gaaccgacgg aaggtgatgg
     6961 ggccgctttg ctcgatctcc catctgatcg catcttcggc tgtcctgcgt cgttccatgg
     7021 gggctccaac ggggttgtgg cgctatggcg acatctggaa gagtagccct cgataccctt
     7081 gcctgcaagg gccgcttcgc cttgcctttc gcttcagggg ataggggcaa ttcctggacc
     7141 cccggctggg aagtccccgc tgcccggttg cagaaggttc ttgcggcgat tctggtctct
     7201 gcgcagaaat ggcccccgga ccttggtccg ggggccatca tgaagcacgc taccctactt
     7261 gccgaagacc agcatcgtcg ccgtttggtt gatgtggccg aaaagctgct ctccataagt
     7321 gctgaagcct acggtcggga ttgtggagaa cagtctaccg tagtcctcgg tcctcccaag
     7381 agcttccagt tcgagggttc ggagaatgca gttgaagttc aacagcgcgg ctatcttgcc
     7441 gaactccttc tccttatctt ctatcgcctt gcgggtgtct gctatcaggt ccgtcgagtc
     7501 tagtatcgag aggctcatcc cctcgaggac gttgcagtag aagacgatgg agtcgccctt
     7561 gacctgctgc ggcgagcgca cgaagggctc gtcgcccgcc atcagcccca gcgggtgctg
     7621 catgaaggag ttcgcaacgt ccttcacttc gacgcccacg gccttggcat atgcctttgt
     7681 ggcgggcatg ttgtcgaact cgaggaccgt tctggtcgcg gtgtccacct tcgtcgcgac
     7741 caactgcttc gcggttggga cgaagctctg tgtcttgatg atgtcgaaac gtgccgacgg
     7801 cttgatgagc gcaagcacag ccgcgtcgct caaggcccga ccgttgacga agacgtaggt
     7861 tgcctcgaac ctcacgtcgt cgccggcgga cccaccgatg aacctgacgt cggttaggtc
     7921 accgatagta tccatcagtc tctcttcgga cccgctgaga ccgtccacca ggatgatgcc
     7981 gacgtacttc tcgaactcca tgcggcgcat cggagtcccg aacttctttc ccaggttggc
     8041 gagggcggca ggcacgcggt tctcctcgcg gacgcgttcc acgacctcta ccgcgatgtc
     8101 ttctacacag tcggcgccga atgccattgc cacgatggag tgtttcagca tgtggccgct
     8161 gacgatctcg ccagctgtag tgcacccgat agtgaccgca tcaccgaagg ctgtctggac
     8221 tgctgcagag agcgtatgcg ggtcgtagac gggggacgcg aagtagagca ccgccttggg
     8281 accgacgcct gcgagcttcg tcgccagttc ctcgactgcc gcagacacag tgtctttcgt
     8341 cgaaaatgcg taaccaatgc tcatcttgat tgaactccct acagacgaat gttgctcaag
     8401 cctagctccc tcgccagact ctcgagtttt tgcatgaccg ccggatcgtc cgctgaggtg
     8461 gggccatagg aggccggatt gattcctttg aggattagct tcgtcgtggt gaccgaagcc
     8521 agtgtggggt tccccttgga cctctcctgg atgatgcgat cgatcatcac cttgatcttt
     8581 cccgccatac ctgtcacgtc tccttgtttc gtcactggat aggaggacag agggctacct
     8641 tcggtagccg tcaagcactc tactcctata tagaataggc agggttggcg ttttctgaag
     8701 gggctggcgg ctgtgttgcc gggggtgagg agaatgtggt ggtacgctcg ggcatgatca
     8761 cgtgcgatac ctgtttggca catctgtacc gtgacttgcc cctacttaat acggaagcta
     8821 tggccgattg tataggtagg acttctgcgt gttccgcagt cgagtcgcca tcagtgggga
     8881 tgtcggccgt atctacagga atgcgccgtg tgtcctggta caggaccacg atgagaagtc
     8941 gggagatgct cctgcctttg ggtccgtggg cccagaaggc aagtgtgtcg aatgaggatt
     9001 ggagaggacg ttctgatgga cgatcagcta gtagtgttcc ggctggctac cgaccactat
     9061 ggagtgggca ttagtgcggt ggagagcatc atcaagatgc agcccatcac ggaggtgccc
     9121 ctggctcagc agttcgtaga gggggtcatc aaccttcgtg ggaaggtggt tccggtaata
     9181 gatctgcgca aacggttcgg cctcggctgc gagcaggttg gaaaggacac gagagtcatc
     9241 gtggtgaatg caggtggcgt caccatcggt atggtggtgg acgaagtgag tgaggtgctt
     9301 cgtgtggacc agggggccgt ggagccgccc tcgccggtag tggccaccgc cgagtccgcc
     9361 ttcgtgaggg gcattgccaa ggtggacgag cgcctgatca tcctcctcga tctcggccga
     9421 attctcactg aagagcagag ggcggatctc gagcagattc ccgccgcggt ttgacccgtc
     9481 ggcccataag ctgcagaccc gtgtcctcgt cgatcgcgcc cgtatcgagc tcgacggccc
     9541 tgcccgtctg agcctcgctt ctgccgtaga ggggctcgat tggtgcgctt cgagacgagt
     9601 ctaagggggg ggtgatcagc ctccaccatc cagatagatc ggctctggag ggtggaggct
     9661 gatcaccccc ctttgtcgga ggtctgacac cctggaccct cctacgcgcc gcttggtata
     9721 attcctagat tgctcttcgc ggtgatagcc gcagtttcgg ctctttgcgg cagacttgga
     9781 gtggtccctt gcctgacttt cagcttgcgt cagatttcgt tcccacgggt gatcagcctc
     9841 aggccatcga acaactggtc cgagggctgc agtccggaat gcactgtcag acccttcttg
     9901 gtgtaacagg gtcggggaag accttcacca tggcgaacgt gatcgctcaa atgcagcggc
     9961 caaccctggt gatggctcat aacaagaccc tggccgccca gttgtgctct gagttcaagg
    10021 agttcttccc ggagaactcg gtagagtact tcgtctctta ctatgactac tatcagccgg
    10081 aggcctacgt tcccaagtcc gacctctaca tcgagaagga ctcatcgctg aacgaggaga
    10141 tcgataggct ccgacacgcg gccactaagg ccctcttcga gcgtcgcgac gtcgtgatcg
    10201 tcgcctctgt ctcctgcatc ttcggcctcg gttcccctga ggactatggg cagacagtgc
    10261 tcaagatcaa gaagggtgag gttcggaagc gagataaggt gctgcgcttc ctgactgaca
    10321 ttcactacga acgaaacgat tacaacttcg ctcggggaaa gtatcgggtg cgtggcgata
    10381 ccctggagat ctacccagtg ggcgaggatc tcgccatcag gatagagttc tggggcgatg
    10441 aggtggagcg gatcaccgag gtagactcgc tgacgggcga ggtgctcgtc gagcgcacgc
    10501 aggtggacgt gtacccagca aagcacttcg tgaccacgca ggagaagctt gaggccgccc
    10561 tt
//
LOCUS       DATALS010000237         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_24721,
            whole genome shotgun sequence.
ACCESSION   DATALS010000237 DATALS010000000
VERSION     DATALS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24721"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VHS06_05350"
     CDS             complement(<1..1244)
                     /locus_tag="VHS06_05350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2987578.1"
                     /translation="MEETNPQARTRVLLSLLAVIVAAAALRLFLLAGPQTELEADEAI
                     VGLMARHILHGERPLFYYMQPYMGSLEAYLIAALFALFGSSTFVLKLVPMLGALLFVA
                     LLFVTGYRLRGLTAAIIAGLFGALPPAFLAIWSLKARGGYIEILVMGQLLILMTMDLG
                     KRGEIGWGRGFLIGAIAGLGLWTNPLIAVYLIPVAIYLILVLRSRVFGWWLVPAAAGL
                     LLGSAPLICYNLATGFATGNLMDGGDGSGLENAPDYLRRTLRLCIPILAGLFQASSSP
                     RLFGDALAANPLNRALFAQSIAVPFVVLLIYGARKLPALLRGHGADRGELLLSLLMVT
                     VPTVFVVTRFREMISEPRYLLPLYSAIPLMGSTLASLRWPRARTIALGAFVLVIVANL
                     IGVARLDPKLNLPDTVVGSTAA"
     gene            complement(1357..2031)
                     /locus_tag="VHS06_05355"
     CDS             complement(1357..2031)
                     /locus_tag="VHS06_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX2987579.1"
                     /translation="MKALEGVFRTPLSGRSAKPRTTGYTMVIDKGTEGAGVLSYPGGT
                     FGEIAVWQDSVTAYLKRVKELGFDGVEVSDGTIEMTDDQRHDFVKRAVDMGFKVVTEV
                     GKKDPNDKVGIPKMHADIKMDLELGAFKVIVEARESGKGVGIFNSAGEPDSQEVENII
                     SGLEDPSVLIWEAPLKNQHIFLIKRFGINVNLGNVPPEEVLVLEALRNGMRGDTLKMA
                     YLGKMK"
     gene            complement(2031..>2269)
                     /locus_tag="VHS06_05360"
     CDS             complement(2031..>2269)
                     /locus_tag="VHS06_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987580.1"
                     /translation="IISDITSQSKLKWMLFNNATEKIGGTNLFYETVVVDTMDTPDLM
                     QKRLKAMERLVSGEVEILRAKNILAGTHNCMEGI"
BASE COUNT          493 a          704 c          658 g          414 t
ORIGIN      
        1 ttggcggcgg tgctgccgac gacggtgtcg ggcagattga gtttggggtc cagccgggcc
       61 acgccgatca gattggcaac aatcactaat acaaatgcgc ccagtgcaat tgtgcgagcc
      121 ctgggccatc tcaaggaggc aagcgtcgag cccatcagcg gaatggccga gtagagaggg
      181 agaaggtatc gaggctccga gatcatctcc ctgaaacggg tgaccacgaa caccgtggga
      241 accgtcacca tcagtaggga aaggagaagt tcaccccgat ccgcgccgtg cccgcgcagc
      301 agggcgggca acttgcgggc accgtagatc aacaacacca caaaaggcac cgcaatcgat
      361 tgggcgaaca aggccctgtt cagcgggttc gccgcaagcg cgtcgccgaa gagcctcggg
      421 gaagagcttg cctggaacag ccccgccagg atcggaatgc agaggcgcaa agtccttcgg
      481 agatagtccg gcgcgttttc caacccgctt ccatcgccgc catccatcag gttccccgtg
      541 gcgaagccgg tggcaaggtt gtagcagata agaggcgcgc tccccaggag tagtcctgcc
      601 gcagccggca caagccacca tccgaatacc cgactcctga gcaccaggat caggtaaatg
      661 gcaacgggaa tgaggtagac ggcgataagc gggttggtcc acaaccccag cccggcgatc
      721 gccccaatga ggaagccgcg cccccacccg atctcgcctc gtttgccgag gtccattgtc
      781 atcaggatca gcaactggcc cataaccaag atctcgatgt aaccgcctcg ggccttgagg
      841 ctccagatgg ccaggaaggc aggaggaagc gcgccaaaca ggccggcgat aatggcagct
      901 gtgagtccgc gcagccgata gccggtcacg aacagaagcg ccacgaacag cagggccccc
      961 agcatcggca ccagcttcag cacgaaggtg gagctgccga acaacgcgaa cagcgcagcg
     1021 atcaggtaag cctccaggct tcccatgtaa ggctgcatgt agtagaacag cggccgttcc
     1081 ccgtgaagga tgtggcgcgc catgagccca actatcgcct catccgcctc taactcggtc
     1141 tgaggaccgg ctagcaggaa gagtcgaagg gcagcggcag ccacaatgac cgcgagaagg
     1201 gaaaggagaa ctctggtgcg agcctgaggg tttgtttctt ccacacttat gtagtccgtc
     1261 ggcaggatgg cgcaatcata aggggcatcc accgaccgag gcaacagtcg gaggctgtag
     1321 gggacggagg gggccggcag ccccctccgc ggagtctcac ttcatcttcc cgaggtaggc
     1381 catcttgagg gtatcgcccc tcatcccgtt gcgcaacgcc tccagcacca gaacctcttc
     1441 gggcgggacg ttgcccaggt ttacgttgat cccgaaccgt ttgatcaaaa agatgtgctg
     1501 gttcttgaga ggggcctccc agatcaagac gctagggtcc tcgagcccgg agatgatatt
     1561 ctccacttcc tgcgaatccg gctctcccgc cgaattaaag atgccgacgc ccttaccgga
     1621 ctccctcgcc tccactatca ccttgaaggc gcccagttca aggtccatct tgatgtcggc
     1681 gtgcatcttg gggatgccca ctttatcgtt tggatccttc ttgccgacct ctgtcaccac
     1741 cttgaatccc atgtctaccg cacgcttgac gaaatcgtgc cgctggtcgt cggtcatctc
     1801 gatggtgccg tcggacacct cgacgccgtc gaaccccaac tccttgaccc gtttcaaata
     1861 ggcagtgacc gaatcctgcc agacggcgat ctcgccaaag gtaccgccgg ggtaggacag
     1921 cacgccggcc ccctccgtcc ccttgtcgat gaccatggta tagccggtgg tgcgaggctt
     1981 cgcgctcctc ccagatagag gagtcctgaa aacgccttcc agagccttca tcagataccc
     2041 tccatacagt tgtgggttcc cgccaagatg tttttggctc gaagtatctc gacctcacca
     2101 gacaccaggc gctccatcgc cttgagacgc ttttgcatga gatccggggt atccatcgta
     2161 tcgacgacta cagtctcgta gaacaggttg gtgccgccga tcttctccgt ggcgttgttg
     2221 aacagcatcc acttgagctt cgactggctt gtgatgtcgc tgatgatgg
//
LOCUS       DATALS010000238         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_417898, whole genome shotgun sequence.
ACCESSION   DATALS010000238 DATALS010000000
VERSION     DATALS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417898"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VHS06_05365"
     CDS             <1..373
                     /locus_tag="VHS06_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2987581.1"
                     /translation="FDGVRVSPEAVLGPERRGIKLCYVTDTRPTPELPEFVQGADLLV
                     CEGMYGSPEDLPKAEGNGHMLFAEAAEIAREGQVRELWLTHYSPSLADPEEWLEEATR
                     IFPNTRAGYDRMTGSLSFPEE"
     gene            complement(399..1439)
                     /gene="namA"
                     /locus_tag="VHS06_05370"
     CDS             complement(399..1439)
                     /gene="namA"
                     /locus_tag="VHS06_05370"
                     /EC_number="1.6.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase NamA"
                     /protein_id="HEX2987582.1"
                     /translation="MAHLFDPITLRGLTLKNRIAMAPMCQYSAGPEGMPNDWHLVHYV
                     SRAIGGVGLVIVEATAVEPVGRISERDLGIWDDRQTEALARIVSLCHERGARIGIQLG
                     HAGRKAWSEKRAFGPEQPVAPSAIPFDEDWNTPRALGAEEIDRIVAAFRKAARRATDA
                     GFDVVELHAAHGYLINQFLSPLSNKRDDQYGGSLEGRMRLLHRVVAEVREIWPERSPL
                     FVRVSASDYTPGGMDIEQMVEIARTLGERGVDLVDTSSGGVAPAAPPSIFPGYQVPFA
                     HRIKRDAGIATGTVGLITRPEMAEEIVRNKRADLVILGRELLRHPYWPLEAARELGVE
                     LDWPIQYRRAKR"
BASE COUNT          258 a          520 c          474 g          298 t
ORIGIN      
        1 gttcgatggg gtgagggtct cacccgaggc ggtgctgggg cccgagcggc gagggatcaa
       61 gctgtgctac gtcaccgaca cccgccccac cccggagctt cccgagttcg tgcagggcgc
      121 ggacctgctg gtctgcgaag ggatgtacgg ctcgccggag gatctgccga aggcggaggg
      181 gaacggtcac atgctctttg cggaggcggc ggagattgcg cgggagggac aagtgcgcga
      241 gttgtggctc acccactaca gtccctcact ggccgatccc gaggagtggc tggaggaggc
      301 cactcgcatc ttccccaaca ccagggcagg ctacgatcgc atgacgggca gcttgtcttt
      361 cccggaggag tagtgaactc gtattccctt cgcgttccct accgttttgc gcggcggtac
      421 tggatcggcc aatccagctc cacgcccagc tctctagcgg cctccagtgg ccagtagggg
      481 tgccgtaaca gttctcggcc gaggatcacc aggtcggccc gcttgttccg cacgatctcc
      541 tctgccatct ccggcctcgt tatgagcccc accgttcccg tggcgatgcc tgcgtctcgc
      601 ttgatgcgat gggcgaaggg aacctggtag cccgggaaga tggatggagg tgcggcggga
      661 gccacgcctc cagagctggt atccaccaga tcaacccccc gctcgcccag cgttcgggca
      721 atctccacca tctgctcgat gtccatgccg cccggagtgt agtccgaggc cgaaacccgc
      781 acgaagaggg gagaacgctc cggccagatc tcccgcacct ccgccacaac cctgtggaga
      841 agcctcattc gcccctcgag cgaccctccg tactggtcat ccctcttgtt ggacaggggc
      901 gagaggaact ggttgatcag gtagccgtgg gccgcgtgca gttccaccac gtcgaagccg
      961 gcgtccgttg ccctgcgggc ggcctttcgg aaggccgcaa cgatccggtc gatctcctcg
     1021 gcacccaggg ctcggggcgt gttccagtcc tcgtcgaagg ggatcgcgct gggggccacc
     1081 ggctgctcag ggccgaaagc ccgcttctcg ctccatgcct tccggcccgc atggcccagc
     1141 tgaatcccga tcctggctcc tcgttcgtgg catagggata cgatccgtgc caatgcctcc
     1201 gtctgccgat cgtcccatat gcccaggtca cgttcgctga tgcgtcctac cggctccacg
     1261 gccgtcgcct cgacgatcac cagccccacg cctccgattg cccggctgac gtaatggacc
     1321 aggtgccagt cgttgggcat gccttcggga ccggccgagt actgacacat gggcgccatg
     1381 gcgatccgat tcttcagggt caggccgcgc agcgttatgg gatcgaacag atgagccatt
     1441 gcctgcactc tacctccagc gagaacaaca gaatcctcat ctcattatag ctctttcact
     1501 tttcgccttt gacctttgac cttgctgcat acgccatgct atgctcgaag
//
LOCUS       DATALS010000239         7146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_417911, whole genome shotgun sequence.
ACCESSION   DATALS010000239 DATALS010000000
VERSION     DATALS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417911"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VHS06_05375"
     CDS             <1..186
                     /locus_tag="VHS06_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987583.1"
                     /translation="LARQAGVEVLTMGSDSHRPTTVGRNLAEAIVLARETGFTHYHTF
                     TARKPVPHFVGATHASP"
     gene            complement(191..754)
                     /locus_tag="VHS06_05380"
     CDS             complement(191..754)
                     /locus_tag="VHS06_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2987584.1"
                     /translation="MNGRGRATNQRRSIRLRGHDYSQPGAYFVTICTLDGRSLLWETA
                     VGQSGLSPCGKIALQCWENIPKHFPLLSLDAYVVMPNHVHGILMISECEPSSPSGGEI
                     SPRLSFSGSRRGSIAAIVGAYKSAVSRQTRGLPCAETGGFWQRGYYERIIRDEAELGR
                     VREYIANNPISWADDPENPLSGKGGMG"
     gene            complement(905..1960)
                     /locus_tag="VHS06_05385"
     CDS             complement(905..1960)
                     /locus_tag="VHS06_05385"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX2987585.1"
                     /translation="MDLIGKQPVPAELLPGARNAVVTCLAIKGGDRVAILTDGETERI
                     GLALREESLKAGAEVEMLFLEQFGQRPFTGMPEKLTEALRNWKPTATFFAARGQKGEI
                     AFRIPMRHVLVDELNVRHGHMIDIDERLMVEGMLANYDVVSQLVHRVTDRVRAAREIK
                     VTSPKGTAMRVRFSPELRWKPCPGIYHTQGEWGNLPEGETFTCPLDAEGVIAADVLGD
                     YFSRKYGVLDTPVIFMLSQGRVVGVRHDNKVLENELRGYLATGENSDRVGEFAIGANL
                     WVKRLSGNLLQDEKIPGVHVAFGDSYPEETGAKWAARTHVDVIPTECTIEVDGRMLMQ
                     DGRFEPDVLAGIQGLPE"
     gene            2267..2812
                     /locus_tag="VHS06_05390"
     CDS             2267..2812
                     /locus_tag="VHS06_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX2987586.1"
                     /translation="MSERMKSIVSIAIAIAVPLAAGGIGTIATAQAIPTWYRGIRKPS
                     WTPPSWVFGPVWTALYTLMGTAAWLVWKTGWKKRSVRTGIGLFGTQLFLNAVWSPIFF
                     GRRAFGAALADLALLWGAILATAIQFYRIRPLAGLLMLPYLLWVSYASTLNAGIWWLN
                     RTRRAEELTSGEAPLREFSSP"
     gene            2809..4224
                     /locus_tag="VHS06_05395"
     CDS             2809..4224
                     /locus_tag="VHS06_05395"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX2987587.1"
                     /translation="MTAAICWFRRDLRLKDNSAVAAAMRSADVVIPVLVLDQTQFSAS
                     LSGEKPLAFFLATMRSLDTELRRRDSYLIVRHGDPVRALGELARQTGARAIFAQEDVS
                     PFGRQRDEKVAERLPLTLVHGISVHPSGAVVRSDGRPYTRYASFRTAWKKLPLPSATD
                     LEHAPRFIPTPSGLSSESIPDRPALPAGVLFTPDEEEAQLRLHGFTEGEDAGITRYDA
                     HRDRLDENGTSQLSPYLRFGMLSPRQAAAAAAAAIGAAKDDAAHSGAESWLDQLIWRD
                     FFLSILYHFPEVLHEAFLPQMRGLPWETDERSLDAWKEGRTGYPIVDAAMRQLSQSGW
                     IHNRARMIVASFLVKDLLIDWKRGERWFMQQLIDGEPALNNGNWQWVAGTGADSMPHF
                     RVFNPILQSRKFDPRGWFIRRWLPEMDHVPEQFIHEPWTMDLHQQKAARCRIGIDYPA
                     PIVDHGFARMRAIELYRSRAA"
     gene            complement(4258..4707)
                     /locus_tag="VHS06_05400"
     CDS             complement(4258..4707)
                     /locus_tag="VHS06_05400"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX2987588.1"
                     /translation="MKAGTRDWLVPILAALGLADAGYLSITHLQGELPACGGYAGCAQ
                     VNDSPFAEVFGVPVALLGAALYLALLAVSLWRLKGPISHRPVATLAIYAMVLSATVVM
                     SYLTALEFFVLHAFCYWCLGLAAITLLLLVLIVSELQRLGALAGVRS"
     gene            complement(4704..5495)
                     /locus_tag="VHS06_05405"
     CDS             complement(4704..5495)
                     /locus_tag="VHS06_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX2987589.1"
                     /translation="MTGKRDKVGRPSQQRPAQKDQGRVRGIAVKNLPAGITTLPARWS
                     LMLVLASLFGILMGVAVVALDLISAAQPPKVSTTAGTTGAVGNQGADRRSKGSANAPV
                     VITEWSDYQCPACRVFALTRESALDEPYVKTSKVRIVYRNYAFLGPESFWAAEAAEAA
                     ADQGRYLEYRNLLWQRQKGENQGTFKIENLKAWAAELGLDMKKFNSSLDGHVYKDAIV
                     AEEKEGETLGIKATPTIMINGRIIDGVPSVDKMKSLIEEELAKKR"
     gene            5708..6748
                     /gene="namA"
                     /locus_tag="VHS06_05410"
     CDS             5708..6748
                     /gene="namA"
                     /locus_tag="VHS06_05410"
                     /EC_number="1.6.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase NamA"
                     /protein_id="HEX2987590.1"
                     /translation="MAHLFDPITLRGLTLKNRIAMAPMCQYSAGPEGMPNDWHLVHYV
                     SRAIGGVGLVIVEATAVEPVGRISERDLGIWDDRQTEALARIVTLCHERGARIGIQLG
                     HAGRKAWSEKQAFGPEQPVAPSAIPFDEGWNTPRALCAEEIDRIVAAFRKAARRALDA
                     GFDVVELHAAHGYLVNQFLSPLSNKRDDQYGGSLEGRMKLLHRVVGEVREVWPERSPL
                     FVRVSASDYTPGGMDIEQMVEIARTLRERGVDLVDTSSGGVAPAAPPSTGPGYQIPFA
                     HRIKREAGIATGAVGLITRPEMADEIVRNERADLVILGRELLRHPYWPLDAARELGVE
                     LDWPIQYRRAKR"
     gene            complement(6774..>7146)
                     /locus_tag="VHS06_05415"
     CDS             complement(6774..>7146)
                     /locus_tag="VHS06_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2987591.1"
                     /translation="FDGVRVSPEAVLGPERRGIKLCYVTDTRPTPELPEFVQGADLLV
                     CEGMYGSPEDLPKAEGNGHMLFAEAAEIAREGQVHELWLTHYSPSLADPEEWLEEATH
                     IFPNTRAGYDRMTGSLSFPEE"
BASE COUNT         1344 a         2363 c         2151 g         1288 t
ORIGIN      
        1 ctggccaggc aagcgggagt ggaggtcctg accatgggct ccgactcgca ccggcccacc
       61 accgttggcc gcaaccttgc ggaagccatc gtcctggccc gtgagacagg cttcacccac
      121 taccacacct tcaccgcccg caagcccgtc cctcacttcg taggggcgac gcatgcgtcg
      181 ccctgagcgt tcaccccatt ccacctttcc ctgacagtgg attctccggg tcgtcggccc
      241 aactgatagg gttgttcgca atatactccc gaacacggcc aagctcggcc tcgtctcgga
      301 tgatgcgctc gtagtaccct cgctgccaga aaccaccagt ttccgcgcag ggcagccctc
      361 tggtctgccg tgacaccgcc gacttgtacg ccccgacgat tgcagctatc gatcctctcc
      421 tcgacccaga aaacgagaga cgaggcgata tctctccgcc actcggtgaa gatggttcac
      481 actcactgat catgagtatg ccatgaacgt ggttgggcat cactacgtac gcatctagtg
      541 aaaggagtgg gaagtgtttg ggtatgttct cccaacactg gagtgcaatc ttgccgcacg
      601 gtgacagtcc tgattgtccc acagccgttt cccaaaggag tgaccggcca tcaagggtac
      661 agatggtaac gaaataggcg cccggttgtg agtaatcatg cccacggagc ctgatggaac
      721 gccgctgatt cgtcgcccgt ccacgcccat tcacagcggt tcccttccaa acaagggcga
      781 cgcatgcgtc gcccctacgt caatcaaaac gatcccgaca gccgaagcaa tcccagcgtc
      841 tcgcgaccac acctgtcaga gcgtctgcgc ctgtcacgcc agccccgccg gtcgcgtcgg
      901 ctccctactc cggcaatccc tggatccccg caagcacatc cggctcgaag cggccgtcct
      961 gcatcagcat ccggccgtcc acctcgatgg tgcactccgt cgggataacg tccacgtgag
     1021 tcctcgccgc ccacttggcc cccgtctcct ccgggtagga gtcgccgaag gccacgtgca
     1081 cgccggggat cttttcgtcc tgaaggagat tgccggaaag ccgcttcacc cacaggttgg
     1141 ccccaatcgc aaactccccc acccgatcgc tgttctcgcc ggtggccagg tagccccgaa
     1201 gctcgttctc cagcaccttg ttatcgtggc gaacccccac gacccgcccc tgagagagca
     1261 tgaagatgac cggcgtgtcc aggacgccat acttacggct gaagtagtcg cccaggacat
     1321 cggccgcgat taccccctcg gcatccaggg ggcaggtgaa ggtctccccc tccggaagat
     1381 tcccccactc tccctgggtg tggtagatcc ccgggcaggg cttccagcgc aactccggag
     1441 agaagcgaac ccgcatcgcc gttcccttgg ggctggtcac ctttatctct ctggccgccc
     1501 gcacccgatc cgtaacccga tggaccaact gggagacgac gtcgtagttc gccaacatcc
     1561 cctccaccat cagccgctcg tcgatgtcga tcatgtggcc atggcgaacg ttcagttcgt
     1621 ccaccagcac gtggcgcatg gggatgcgaa aggcgatctc ccccttctgg cctcgagccg
     1681 caaagaaggt cgcggtaggc ttccagttcc gcaacgcctc ggtcaacttc tcgggcatcc
     1741 cggtgaatgg gcgctggccg aactgctcca ggaacagcat ctccacctcg gcccccgcct
     1801 tcagcgattc ctccctaagc gccaggccga tgcgctcggt ctctccatcc gtgagaatgg
     1861 cgacccggtc gccccccttt atcgccaggc aggtaaccac ggcgttgcgt gccccgggca
     1921 gaagctcggc cggaaccggt tgcttcccaa tgagatccac cttgctgcct ccctattgga
     1981 tgatgcggcg aacagttcaa acggcatatc gaggcgagag gccccgcgca gccaaccaca
     2041 tctctcctat gctaaatccg ggccaattat atcccacccc gaagtagatg ccggcgcgtg
     2101 ccggatgggg cccccggtgc cttcgctgcg acctccggat acgtggcaat cgcgggcagg
     2161 tgtatatgag gcacgtccat tgcagtacta ttcgggatgg aatcccgcgc cgttttctgc
     2221 cctgatacgt ctaccgcctg ccgaaccaag cagggaggac aagccgatgt cggagagaat
     2281 gaagagcatc gtgtccatcg cgattgcgat agcagtcccg ctggcggcgg gaggcattgg
     2341 cactatcgcg accgctcagg ccatccccac ctggtaccgg ggcatccgaa agccctcctg
     2401 gaccccgccc agctgggtct tcggccccgt gtggaccgca ctctacaccc tcatgggcac
     2461 tgccgcctgg ttggtgtgga agaccggatg gaagaaacgc tcggtccgca ccggcatcgg
     2521 cctgttcgga acacagctgt tcctaaacgc cgtatggtcc cccatcttct tcggcaggcg
     2581 agcattcggc gctgcactgg cagatctcgc cctgctgtgg ggcgcgatcc tcgcaaccgc
     2641 aatccagttt taccggatac gcccgctggc tgggctgctg atgctcccct acctgctgtg
     2701 ggtgagctac gcctccaccc tcaacgccgg aatatggtgg ctaaatcgga ctcgccgcgc
     2761 ggaagagctg acttccggcg aagcgccgct tcgggagttt tcctccccat gaccgcggcc
     2821 atctgctggt tcagaagaga cctccggttg aaggacaatt ccgccgtcgc ggcagcgatg
     2881 cgctccgcgg atgtcgtcat accggtgctc gtgctggacc aaacgcagtt ctctgcctcg
     2941 ctgtcgggtg agaaaccgtt ggctttcttt ctggcaacca tgaggagcct ggataccgag
     3001 ttgcgcaggc gcgatagcta cctgatcgtc cggcatgggg atcccgtcag ggcactgggg
     3061 gaactggcca gacagacagg ggcgagggct atcttcgccc aggaagacgt ctcccccttc
     3121 ggccgccagc gggacgagaa ggtggccgag cggctcccgc tcacgctggt ccacggcatc
     3181 tccgttcacc catcgggggc agttgtcagg tccgatggac gaccgtacac ccgctacgcc
     3241 tccttccgca cggcctggaa aaagctgccg cttccgagcg ctacggactt ggagcatgca
     3301 ccccgcttca ttccgacacc ttccggtctg tcctcagagt ctatccctga tcgaccggcg
     3361 ctgcccgcgg gggtgctctt cacccctgat gaggaggagg cccagttgag gctgcacggc
     3421 ttcaccgagg gcgaggatgc gggcataacc cgatacgatg cccatcgcga tcggctggac
     3481 gagaacggca cctctcaact ctccccctac ctgcgcttcg gtatgctgtc tccgcgccag
     3541 gcagcggcgg ccgcagcagc tgccatcggc gccgccaaag atgatgcggc ccacagcggc
     3601 gcggaaagct ggctcgacca actgatctgg cgcgacttct tcctgtccat cctgtaccac
     3661 ttccccgagg tgctgcacga agcctttctg ccgcagatga ggggactacc ctgggagacg
     3721 gatgagagaa gcctggatgc ctggaaggag ggccggacgg gctaccccat cgtcgacgcg
     3781 gccatgcgac agctatccca gagcggctgg atccacaaca gggcccgcat gatcgtcgct
     3841 tccttcctcg tgaaggacct gctgatcgat tggaagcggg gggagaggtg gttcatgcag
     3901 caactgatag acggggagcc ggccctcaac aacggcaact ggcagtgggt ggcggggacc
     3961 ggcgccgact caatgcccca cttccgggtg ttcaacccca ttttgcagag ccgcaagttc
     4021 gatccccgcg gctggttcat tcgcagatgg cttccagaga tggaccacgt gccagagcag
     4081 ttcatccacg agccgtggac gatggatctc caccagcaga aggccgctcg ctgtcgtata
     4141 ggtatcgact acccggcccc catcgtggac cacggctttg ccaggatgcg ggcaatagag
     4201 ctgtaccgca gccgtgcagc ttgaggtacg cgatctagcg aaacgccgca ctccacatca
     4261 gctgcgcaca ccggcaaggg cgcccagcct ctgcaactcg ctcacaatca acaccagcag
     4321 cagcagggtg attgctgcaa ggcccaggca ccagtagcag aaggcgtgca ggacgaagaa
     4381 ctcgagggcc gtgaggtagg acatcaccac ggtggcagag agcaccatgg catagatggc
     4441 tagggttgcc acgggcctgt gggaaatcgg ccctttcaat ctccagagag agacggccag
     4501 tagcgccagg tagagagcag ccccgaggag tgcaacagga acgccgaaaa cctcagcgaa
     4561 ggggctgtcg ttcacctgag cgcacccggc gtatccaccg cacgcgggca actcgccctg
     4621 caggtgtgtt atcgacaggt atcccgcgtc ggccagcccg agcgcggcca ggatcggcac
     4681 cagccagtct ctcgtccctg ctttcaccgc ttcttcgcca gctcctcttc gatcaaggac
     4741 ttcatcttgt ccacgctcgg cacaccgtcg atgatccggc cgttgatcat gatggtggga
     4801 gtggccttga tacccagggt ctccccctcc ttctcctcgg cgacgatcgc atccttgtag
     4861 acatggccat ccaggctgct gttgaacttc ttcatgtcca ggccgagctc ggccgcccac
     4921 gccttcagat tctctatctt gaaggtcccc tggttttctc ccttctgccg ctgccacagg
     4981 aggtttcggt actccagata gcgaccctgg tcggcagccg cttctgccgc ctcggccgcc
     5041 cagaaggact ccgggcccag gaaggcgtag ttgcgataga cgatgcgcac cttgctcgtc
     5101 ttgacgtatg gctcatccag cgcggactcc ctggttaagg cgaagacccg acaggcggga
     5161 cactggtagt cagaccactc ggtgatgacc acgggggcgt tggcacttcc cttggagcga
     5221 cgatcggctc cctgattgcc gacggcgccg gtggttccag cagtcgtgga gaccttaggg
     5281 ggttgggcgg cggagatcag atccagcgcg actaccgcca cgcccatgag gataccgaac
     5341 agtgaggcca gcacaagcat cagcgaccag cgggccggca gcgtagtaat cccggcaggg
     5401 aggttcttga cggcaatgcc tcgaactctc ccctggtcct tttgggcggg ccgctgctga
     5461 ctcggccggc cgaccttgtc tctctttccg gtcatcaccc aacggactcc tgctgaagtg
     5521 gatccagatc accaatggga gggacagtat gccgcgcaca taggatcgct taaggaccgc
     5581 ctcaggtcac gtacctcttc gagcatagca tggcgtatgc agcaaggtca aaggtcaaag
     5641 gcgaaaagtg aaagagctat aatgagatga ggattctgtt gttctcgctg gaggtagagt
     5701 gcaggcaatg gctcatctgt tcgatcccat aacactgcgc ggcctgaccc tgaagaatcg
     5761 gatcgccatg gctcccatgt gtcagtactc ggccggtccc gaaggcatgc ccaacgactg
     5821 gcacctggtc cattacgtca gccgggcaat cggaggcgtg gggctggtga tcgtcgaggc
     5881 gacggccgta gaaccggtag ggcgcatcag cgaacgtgac ctgggcatat gggacgatcg
     5941 gcagacggag gcattggccc ggatcgtcac cctctgccac gagcgaggcg ccaggatcgg
     6001 gattcagctg ggccatgccg gccggaaggc gtggagcgag aagcaagctt tcggcccgga
     6061 gcagccggta gcccccagcg cgatcccctt cgacgagggc tggaacacac cccgagccct
     6121 gtgtgccgag gagatcgacc ggattgttgc cgccttccga aaggccgccc gcagggcatt
     6181 ggacgccggc ttcgacgtgg tggagctgca cgcggcccac ggctacctgg tcaaccagtt
     6241 cctctccccc ctgtccaaca agagggatga ccagtacgga gggtcgctcg aggggcgaat
     6301 gaagcttctc cacagggttg tgggggaggt gcgggaggtc tggccggaac ggtcgcccct
     6361 cttcgtgcgg gtttcggcct cggactacac ccccggcggc atggacatcg agcagatggt
     6421 agagattgcc cgaacgctgc gcgagcgggg ggttgatctg gtggatacca gttccggagg
     6481 cgtggcgccc gccgcacccc cgtctaccgg cccgggctac cagattccct tcgcccatcg
     6541 catcaagcga gaggccggca tcgccacggg agcggtggga ctgataacga ggccggagat
     6601 ggcggacgag atcgtgcgga acgagcgggc cgacctggtg atcctcggcc gagaattgtt
     6661 acggcacccc tactggccgc tggatgccgc cagagagctg ggcgtggagc tggattggcc
     6721 gatccagtac cgccgcgcaa agcggtaggg aacacgaaga gaatacgagt tcactactcc
     6781 tccgggaagg agaggctgcc ggtcattcga tcgtagccgg ccctggtgtt ggggaagatg
     6841 tgagtggcct cctccagcca ctcctcggga tcggccagcg agggactgta gtgggtgagc
     6901 cacaactcgt gcacctgtcc ctcccgggca atctccgccg cctccgcaaa gagcatgtga
     6961 ccgttcccct ccgccttcgg caaatcctcc ggcgagccgt acatgccttc gcagaccagc
     7021 aggtccgcgc cctgcacgaa ctcgggaagc tccggggtgg ggcgggtgtc ggtgacgtag
     7081 cacagcttga tccctcgccg ctcgggcccc agcaccgcct cgggtgagac cctcacccca
     7141 tcgaac
//
LOCUS       DATALS010000240         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_249061, whole genome shotgun sequence.
ACCESSION   DATALS010000240 DATALS010000000
VERSION     DATALS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249061"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..554)
                     /locus_tag="VHS06_05420"
     CDS             complement(159..554)
                     /locus_tag="VHS06_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987592.1"
                     /translation="MPYLHPWYGPDQWAGFIGAKMTMMPETDTSWVEPFVDDWDAQGA
                     LAIAPDNPWWNGVLELAESAAERGEGKFLVSTIDTHSNLDCLSAIRGPSQLCIDLIEN
                     PGGCASGARTDRQALRARLRRRVESRPHG"
     gene            1673..>3548
                     /locus_tag="VHS06_05425"
     CDS             1673..>3548
                     /locus_tag="VHS06_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX2987593.1"
                     /translation="MDTLAARVLCVAVLATILSLAVLSSPLYARTIFVNCTRPGNEGD
                     GLTWATARKTVQAGLNSATSGDQVWVARGTYLERITLGDGVAVYGGFVGTESDLSQRP
                     AFPRPPSDPDEAILDGYRGGSVVTSPPGATAATRLDGFTIRNGSDAGAHGGGICCESS
                     SPTISNNLIKENTAGMGGGILCVLSSPTITNNIIAANVGNYGGGGICCAQFSSPFISG
                     NTIIGNHGSYGGGINCDRSSPTVSSNRITANLAGSGGGVLCQNAASPILSSNAILGNI
                     AVWDSAVCCNGSSPTISNNTIAGNGGGGIDCGSSSPSVSNNIVAMNAWGIRSSGGVPV
                     LQNNDVYNPGGDDYSGLDAGVGDRSVDPRLTGLQYGSCRIHRESLCRNAGFSGAPGVG
                     STDIDGQPRDDGGGVDIGADESYGEDPATVSVVVRVDGTNGSDSNSGSSWALAKRTVR
                     SAIEAACGTGGEVWVMAGTYHERVKLEPYAHVYGGFSGTETARDGRNWTANPTILDGG
                     GGGSVVTASYIGNRTSTIDGFTIRNGKSQDGAGVYCSWCSPTISNNVIRENSAAGGQG
                     GGLLCTGAAPDVSGNAVAQNSSAYGGGIFIELGSPSICGNAIVRNSATYGAAIYCRSS
                     C"
BASE COUNT          705 a         1085 c         1095 g          663 t
ORIGIN      
        1 gatggtatac ggcgtgatcc aggcaagata tctcgtactc cagtgatggc agcacgaatg
       61 tcttgaagtg ctcgggggag accatatagg agaaatcgca ctggactgtc tgtgtgcggc
      121 cctcgctgta gatgtcggac cacgacgtgc tgccacgctc agccatgcgg cctgctttcc
      181 acacggcgtc gtagacgggc tcgtagagcc tgtctatctg tccgagcgcc cgaagcacac
      241 ccccccggat tctcgatcag atcaatgcac agctgcgagg gtccgcgtat tgccgagagg
      301 caatccaggt tggaatgtgt gtctatcgtg gagaccagaa acttgccctc acctctctca
      361 gcagcggatt cagcaagctc cagtacaccg ttccaccacg gattatcggg ggcgatggcg
      421 agagcgccct gagcatccca atcatcgacg aacggctcga cccaagaggt gtctgtctcc
      481 ggcatcatgg tcatctttgc cccgatgaag cccgcccact gatccggccc gtaccagggg
      541 tgcaggtaag gcattgattc gccaccgaag aacgtgcatg acgcccactc ctcaaactcc
      601 cctatcgcag gctcgaagct gcgactgtgt ggagcgatca gcgtgcgctc gggctgaagc
      661 atgacggacc gaggagtccg tactgaaacg caagggcggt ctatcatctc tcctgcccag
      721 tacgcccgca atcgttgttg agcctcggcc gcatctggct tatacgccag atcgagcggc
      781 gtggcattac gagccatctc actcaatgcg caacctcgca gtctgccatt cgcaccgact
      841 accccagcct cgcctgcacc tcagtgatcg actgctcagc gatgcccgtc gcttctgctg
      901 ctccgttggg catcgtcggg gcgatatcct cagaacgtga tccgcaggtg tcgacgccag
      961 gctcgtgcgg aagggtgttt gatagccagc ttcgccgcca cacggaacgg cgccttggtc
     1021 cgatcgctct ccaccagttg gatggcacct gacatgtcag gtgccacaac ggcatacttc
     1081 ggtgacccac tccccaatct cctgcccaag gggccgcagt tcaaagcccg tgccggtatg
     1141 ctgtcagccg ctgccggggc cgaggactgg gcacaggacc tctaccatag gcacgggtca
     1201 cagaaggggc cgcatggata gcagcagccg gcatgtgagg ccggggggtt cgccagtgag
     1261 tggacaggag cgccgccaga ccgttctgat gtgagagtta ggagcaaggg ctgtgcgtgt
     1321 cacgtggaat aggcagtcgc tccgccaacg tcgcatcgtg gacgcgcgtt gccgccaatg
     1381 aagctgatca tggacgaggt cacgttctgg tgcgatgacg gccgtagggt catgctcacc
     1441 aagtgtctgc gcaacaaggc ggatcagtcg ctccggccgt cgccagcacc ttcgcccgcc
     1501 ggttctcttc cctgctcaat cctaccgcct ccctgcccat ccatagatca tgccacctcc
     1561 acccaacccc aaccaggaac ccagaaacgc cattgacata cggactgtcc cagagtagac
     1621 tcccactgga ctcagagcac acgtcattgc tgcgtcaagg gagactgcaa ctatggacac
     1681 actggccgct cgtgttcttt gtgtcgccgt tttggcgacg atcctctcgc tcgccgtcct
     1741 ctcatcgcca ctgtacgcgc gtacaatctt cgtcaactgc actcgccccg gcaatgaggg
     1801 cgatgggctg acatgggcca ctgccagaaa gacggtacaa gccggactca actcggccac
     1861 ctccggcgat caggtgtggg tggccagggg gacctatctc gagcgcatca cccttgggga
     1921 cggagttgcg gtttacggtg gattcgtcgg aacagagagc gacctcagcc agcggccggc
     1981 gttcccaagg ccgccctcgg atccggacga ggcgatcctc gacggctaca gagggggcag
     2041 cgtggtgacc tcgccgccag gcgcgactgc agctaccagg cttgacggat tcaccatccg
     2101 taacggcagc gatgccggcg cccacggcgg cggcatttgt tgtgagagct catcgcccac
     2161 gatatcgaac aacctgatca aagagaacac agccggtatg ggcggcggca tcctgtgtgt
     2221 gctcagctca cctactatca ccaacaacat catcgctgcg aatgtgggaa actacggtgg
     2281 tggtggcata tgctgcgcgc agttctcgtc accattcatc tcgggcaaca cgatcatagg
     2341 aaaccacggc agctacggcg gtggcatcaa ctgtgatcga tcgtcaccaa cggtgagcag
     2401 caacaggatc acggccaacc tcgcaggcag cgggggcggt gtgctgtgtc agaacgccgc
     2461 atccccgatt ctgtcgagca acgcgatact gggcaatatt gccgtctggg actctgcagt
     2521 gtgctgcaac gggtcttcac cgacgatctc aaacaacacg atcgcaggga acggcggggg
     2581 tggtatcgac tgcggctcgt cgtcgccttc ggtttcgaac aacatagtcg cgatgaatgc
     2641 ctgggggata cgcagttcgg gcggcgtgcc agtcctccag aacaacgatg tctacaatcc
     2701 cggcggagat gattactcgg ggcttgacgc gggcgtgggt gatcggtcgg tggacccaag
     2761 gctcacaggc ttacagtatg gcagctgccg gatccatcgg gagtcgctct gcaggaacgc
     2821 ggggttcagc ggcgcgccag gcgtcggctc gacggacatt gacgggcagc ccagggatga
     2881 cggcggcggg gttgacattg gagcagatga gtcatacggc gaagacccgg cgaccgtgtc
     2941 ggttgttgtg cgggtcgatg ggaccaacgg cagtgactcc aacagcggat caagctgggc
     3001 tctggccaag agaacggttc ggtcggcgat cgaggctgct tgtggaactg gcggcgaagt
     3061 ctgggtgatg gcaggcacct accacgagcg ggtgaagctg gagccctacg cgcatgtcta
     3121 cggcggattc tcgggcacag agaccgctag ggatgggcgc aactggacgg cgaaccccac
     3181 gatcctcgat ggcggtgggg gtggcagcgt agtgactgct tcatacatcg gcaaccgcac
     3241 cagcaccata gacggcttca ccatccgcaa tggcaagtcc caggacggcg cgggtgtata
     3301 ctgctcctgg tgctctccca cgatatccaa caacgtgatc agggaaaact ccgctgcggg
     3361 cggtcagggc ggtggtcttc tctgcacggg ggctgccccg gatgtctctg gcaacgctgt
     3421 agcgcagaac agctctgcct acggcggagg gatcttcatc gagttgggct cgccgagcat
     3481 ttgcggcaac gcgatcgtca ggaacagcgc tacctacggt gctgctatct actgccgttc
     3541 gtcgtgtt
//
LOCUS       DATALS010000241         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_326513, whole genome shotgun sequence.
ACCESSION   DATALS010000241 DATALS010000000
VERSION     DATALS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326513"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1630
                     /locus_tag="VHS06_05430"
     CDS             113..1630
                     /locus_tag="VHS06_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2987594.1"
                     /translation="MVIGDGEDIHLRKAVSVEITAVRGFSSLRALVVGDAMLDSYYLG
                     SASRLSPEGPVPVIRSHEKHHLPGGAANTAANIRALGARTVLLSVVGPDHEGELLRSQ
                     LSRMGVDVTHLMVDPRRPTTSKLRILGDNQFLARFDEEEDSDLEGETEADFLARFRGL
                     FGESDVVVVSDYMKGTITPALVAELSRLNQGGDRPVVVDSKDLVRRGFHNVSVITPNH
                     LEAQRAISRREELDGTRGKGPNPEELGRRLLDAVGTRWVIVTLGSDGALLFEREQPTV
                     RVTAREVRSPDAVGAGDSFTAALALGLASGLEIEVASRIAVEAAAISVGKRMTAVVEQ
                     QELLQRLSLSVGDVPAGSLADRLEKYRSDGARLVFTNGAFDGLHSGHIDFLKEARRLG
                     DVLIVGVNSDESMRRRTGQSPRHSEEERLSAVASLESVDHSLLFDEETPSALIREIRP
                     QIHVKGGDYQEVELPEAEAVREVGGQVVILPLLERRSSRLLSGKVTRSVRRSRAA"
BASE COUNT          292 a          438 c          601 g          307 t
ORIGIN      
        1 cgctttggca cggtttagcc acgctccgcg acgtttatcg ccgattgcgt tcatttgtgc
       61 acaaatggca cacctcttgc ccttaggcgg gacctgtccc gatgaggtcg cagtggtgat
      121 cggggatggc gaagacatcc acttgaggaa ggctgtgagc gtcgagatta ccgccgttcg
      181 tggtttttcg tccttgcggg cacttgtggt gggcgatgcc atgctggaca gctactacct
      241 tggcagcgcc agccgtcttt cgccggaagg ccctgtgccg gtgatccgct cgcacgagaa
      301 gcaccacctg ccgggtggcg cggcgaatac ggccgccaat atccgcgccc tgggggcaag
      361 gaccgttctc ttatccgtcg tcggcccgga ccacgagggt gaactgctgc ggagccagtt
      421 atcgaggatg ggagtagacg ttacccatct catggtcgat ccgcggaggc cgaccacgag
      481 caagctccgg atccttgggg acaaccagtt cttggctcgg ttcgacgagg aggaggactc
      541 cgatcttgaa ggagagacgg aggcagactt cctcgctcgt ttccgaggac tgtttgggga
      601 gagcgacgtg gtggtggtgt cggactacat gaagggcacg atcacgccgg cgctggtggc
      661 agagttgagc aggctgaacc agggcggtga tcggcctgtg gttgtggact ctaaggatct
      721 tgtgaggcgc ggcttccaca acgtcagcgt cataacccca aaccacctcg aggcgcagcg
      781 ggcaatcagc cgccgagagg agctagacgg tactcggggc aagggaccga accctgagga
      841 gttgggcagg aggttgctgg atgcggtcgg caccagatgg gtcatcgtca cgctaggatc
      901 ggatggagct ctgctgttcg agcgggagca gccgactgtt cgggtaacgg ctcgcgaggt
      961 gcggagcccg gatgccgtgg gggctggcga tagcttcacg gccgcactgg ctcttggtct
     1021 ggcttcaggg ttggagatcg aggtcgcttc caggatcgcc gtggaggccg ccgccatttc
     1081 ggtcgggaag cgtatgacgg ccgtcgtgga gcaacaggaa ctgcttcagc ggctcagctt
     1141 gagtgtcggg gacgtgccgg ccggaagcct cgccgatcgg ctggagaagt acaggtcgga
     1201 tggtgcaagg ctggtgttca ccaacggggc cttcgacggg ctgcacagcg ggcacatcga
     1261 cttcctaaag gaggcgagga ggctgggcga cgtgctgatt gtgggagtca actccgacga
     1321 gagcatgcgc aggcgaaccg gccagagccc aagacactcg gaggaggagc ggctttccgc
     1381 ggtggcgtcg ttggagtcgg tggaccatag cctgctcttt gacgaggaga ccccgagcgc
     1441 gctgattcgg gagattcgtc ctcagatcca tgtgaagggg ggggactacc aggaggtgga
     1501 gttgccggag gcggaggcgg tacgcgaggt aggtggccag gtagtcatcc ttccgctact
     1561 ggagcggcgg tcgtcccggc tcctgagtgg aaaggtcacg cggtctgtgc ggcgctcccg
     1621 tgcggcgtag aggaggag
//
LOCUS       DATALS010000242         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_159081, whole genome shotgun sequence.
ACCESSION   DATALS010000242 DATALS010000000
VERSION     DATALS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159081"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VHS06_05435"
     CDS             complement(<1..904)
                     /locus_tag="VHS06_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX2987595.1"
                     /translation="MDKDWLLELYRRMVRIRKFEDGVIQLYTRGIMPGLAHCYPGEEA
                     VAVGVCAALRQDDYITSTHRGHGHLIAKGGKTDRMMAEVLGKAAGYNKGRGGTMHIVD
                     LSLGILGANGIVGGSIGIAAGAGWSIQQRGTDQVCVCFYGDGASNQGVTLESMNLSKI
                     WNLPVIYVCENNLYGMYTAASRVVGNSLTARAQGVGLRATKLDGQDVLGVYDAMVQVV
                     DRVRNGGGPEFVECMTYRRYGHHVGDQGKAYRPAEEIAEWNNNRDPLKLLSARLLADR
                     VCTAAEIEAVENEIQKEVDDAVEFA"
     gene            complement(982..>1933)
                     /locus_tag="VHS06_05440"
     CDS             complement(982..>1933)
                     /locus_tag="VHS06_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX2987596.1"
                     /translation="ADTAAIGSIMLPSMVRRGYPRPLATAIVAAAGGMGILIPPCILM
                     IIFALAANVSVGYLFAAGFVPGALMGLSLMVMVYMVAGREGLPREERSNCGEMFRAFV
                     EAVPPLLMPVIILGGIISGIFTATESAVVAVLYGALLSLLVYRELHLSDIPRILLDSA
                     KLTGVVTLLIGMSSTFAWILATQQIPQAIASGMLSISKEPWAFLMLVNILLLFVGTFM
                     DATPAVIILVPILFPVATKLGIDPIHFGIVMVANLGIGFITPPVGTCLYVAAGIGKVS
                     IEQLVKPLLPFLAMMILTLIIITYMPDVTLFVPRLTGYSR"
BASE COUNT          391 a          615 c          581 g          346 t
ORIGIN      
        1 tggcgaactc caccgcgtcg tccacttcct tctggatttc gttctctacg gcctcgatct
       61 ctgcagccgt gcagacccgg tcggccagca gccgggcaga caacaacttg agcgggtctc
      121 ggttgttgtt ccactcggcg atctcctccg cgggacggta tgccttcccc tggtcgccga
      181 cgtggtggcc gtagcgccgg taggtcatgc actccacgaa ctcgggcccg ccgccgttgc
      241 gcactcgatc caccacctgg accatggcat cgtagacacc cagcacgtcc tggccatcca
      301 atttggtggc gcgcaacccc acgccctggg ccctggcggt caggctgttg cccaccactc
      361 gggatgcggc ggtgtacatg ccgtacaggt tgttctcgca gacgtagatc accggcaggt
      421 tccagatctt tgacagattc atcgactcca gggtcacccc ctggttggaa gccccgtcgc
      481 cgtagaagca gacacacacc tgatcggtgc cccgctgctg gatggaccat ccggctcctg
      541 ccgcaatgcc gatgctcccg ccgacgatgc cgttggcccc caggataccc agggagagat
      601 cgacgatgtg catggtgcct ccgcgaccct tgttgtagcc ggccgccttc cccagcacct
      661 ccgccatcat ccggtccgtc ttgccgccct tggcaatcag gtggccgtgc cctcggtggg
      721 tgctcgttat gtagtcgtcc tgtctcaggg cggcgcagac gcccacagcc accgcttctt
      781 cgcccgggta gcagtgggcc aggcccggca tgatccctct tgtgtacagc tggatcacgc
      841 catcttcgaa cttgcgaatg cgaaccatcc gccggtagag ctcaagtagc cagtccttgt
      901 ccatgttgcc tccggatcgt agatccttcg agagtagggc cggggtcgca ttgccaagag
      961 gacgacaggt cccctcagcc gctagcgact gtacccggtg agtctaggaa caaaaagggt
     1021 cacatcaggc atataggtaa tgatgatcag cgtcaggatc atcattgcca ggaagggcag
     1081 cagtggcttc accaactgct cgatggaaac cttgccgatg cccgcggcca cgtagaggca
     1141 ggtgccgacg ggcggagtga tgaacccgat gcccaagttt gccaccatca ctattccgaa
     1201 gtggatcgga tcgatgccga gtttcgtggc cacagggaac aggatgggca ccaggatgat
     1261 gactgcgggc gtggcatcca tgaaagtccc cacgaacagt agaaggatgt tcaccagcat
     1321 caggaacgcc cagggctcct tcgatatgga aagcatcccc gaggcgatcg cctgcgggat
     1381 ctgctgagtt gccagaatcc atgcaaaggt ggatgacatg ccgatcaaca acgtcaccac
     1441 acccgtgagc ttggccgaat ccaggaggat gcgaggaatg tcgctgagat gcagttccct
     1501 gtacaccagc aaggagagaa gggcgccgta gagcacggca accaccgcgg actcggtcgc
     1561 ggtgaagatc cccgagataa tgccgccgag gatgatgacc ggcatcagca gcgggggaac
     1621 ggcctcaacg aaggccctga acatttctcc gcagttggag cgctcctccc tgggaaggcc
     1681 ctcccgtccg gcgaccatgt agaccatcac cataagggat agccccatca gcgcgccggg
     1741 aacaaaaccc gccgcgaaga ggtagcccac cgagacgttg gcggcgaggg cgaagattat
     1801 catcagaatg cagggaggaa tgagtatacc catgcccccg gccgcagcga cgatggccgt
     1861 cgcaaggggt cgaggatagc ccctcctcac catggacggg agcatgatgg agccgatggc
     1921 cgcggtgtct gcc
//
LOCUS       DATALS010000243         9079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_56643,
            whole genome shotgun sequence.
ACCESSION   DATALS010000243 DATALS010000000
VERSION     DATALS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9079
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56643"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VHS06_05445"
     CDS             <1..932
                     /locus_tag="VHS06_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2987597.1"
                     /translation="LLSSEKDSHSEPRTQNSEPEAVPLPLRPPVLCPGCPHTGAFYAL
                     KRLGFYRGTGEVSQNLPGQILAKLKRSGVVISGDIGCYTLAVLPPLLAMDSCGCMGAS
                     IGGAMGMEKAGLANKVVAVIGDSTFLHSGMTPLLDVVYNGGTITTIILDNSTTAMTGH
                     QEHPGTGRSVTGAHARKVNLETLVRGLGVEDVKVVNAFAVDEVSAAIRDSVERDEPSV
                     VIVKGECSLKAKVAGEPFVVDPALCDGCGACLRTGCPAIVLRDEKAEIQAGICVGIEC
                     GVCAQVCPKGAILAPGVAGQRTSDRGMGRSERG"
     gene            934..1530
                     /locus_tag="VHS06_05450"
     CDS             934..1530
                     /locus_tag="VHS06_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HEX2987598.1"
                     /translation="MDFLLAGVGGQGIVLMSDLLAEAALEAGYDVKKSDVFGMAQRGG
                     SVTSYVRIGEKVYSPLPRPGEVDFLVGLEKLEAARWASHLVPGGTAVVNDYSLFPLEV
                     SSGAERYPTDQQVIGSFESRGCRVHLVDGTGMARQLGNPKVLNVAVLGQLASLLPLPW
                     EAWTRTVERRIPARFRELNLRALALGQETARSELRTAD"
     gene            1668..2966
                     /locus_tag="VHS06_05455"
     CDS             1668..2966
                     /locus_tag="VHS06_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX2987599.1"
                     /translation="MYWDQQHETLPRAEMEALQVERLRRKVREVYEKVPFYRNAFQER
                     GITPEDISSLDDLTKLPFTNKADFRDNYPFGLLTIPLKDVVRVHGSSGTTGKPTVVAY
                     ARADLELWADLMARGMTMGDVTDEDIVQNAYGYGLFTGGLGFHYGAERIGATVIPISG
                     GNTKRQIMLMQDMKTTVLTCTPSYSLFLAETAREMGVDVRELPLRVGFFGAEPWSYRM
                     RQEIEEALGILALDTYGLSEIIGPGVAQECPHKRGLHIYEDHFLPEIVDPVTGESVPD
                     GVKGELVFTTLTKVALPVIRYRTRDITSLHREKCDCGRTLVRMEKVTGRTDDMLIVRG
                     INVFPSQIESVLLEVDGVDPHYQIVVDRARGLDDLEIQVEVSETVFSDEIGRLESLTK
                     KVRVEIESTLGIQALVKLVEPGAIPRSEGKAKRVIDRREL"
     gene            2992..3378
                     /locus_tag="VHS06_05460"
     CDS             2992..3378
                     /locus_tag="VHS06_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX2987600.1"
                     /translation="MKQVSVFAENRAGRLKTLLAVLARAEINIRALSIADTADFGIVR
                     MILSDTDGGLEAIRAAGFAASTNDVLRVEVPDQPGGLLQAVAEPLAAAGVNIEYVYAF
                     ADNPEKHAMVVLKVGDIDAAERLLSK"
     gene            3381..5255
                     /locus_tag="VHS06_05465"
     CDS             3381..5255
                     /locus_tag="VHS06_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX2987601.1"
                     /translation="MSSFLGKLLVVDLSARAAREVSLDPELCRRYLGGIGMATKLLWD
                     YSRPRTDPFSPENPLVFAAGPLAGTMAPSGSKYAVATKSPLTGFIGDSLSSGGWPLAL
                     KRAGYDAMVVQGAADVPTYVFVDDDRVYFRSARRLWGGKCWETEEAIRRELGDDGVRV
                     ATVGPAGERLVRYACIGNDYGRQAGRTGPGAVMGSKNLKAIAVRGTKPVGVSDPGRVK
                     AASLDLYARSQGAATTKYRVLGTPANVLALDALAALPAYNFRQSTFEKANRVSGEYFY
                     ERFKAKVVACAGCPIGCDHIYRVTDGQYAGTEVGMDYESLYALGPLCGIDDAAAILKA
                     SELCDQYGLDTMSTGASIAWAMESYERGLISRDEAEGLDLRFGSAEAVVTMVEQIGLR
                     RGLGRLLGEGVRRAAATLGSGSDFWAMHVKGLELPGYEPRSLKTLALGLAVGTRGACH
                     NRSAGYEVDLSAKVDRLSSERGRGPLAMAQEDYAATLDSLGVCKFLRRCFDAFHVEAA
                     DLFRATTGLELTAEELQKVGERVSNLKKAYNIREGWTRADDTLPPRILEEKLPTGVAR
                     GTGLTRSELDEMIDGYYEARGWTTEGLIPPAKLAELGMEELGAGVSEGASDAKTLHSM
                     "
     gene            5230..5757
                     /locus_tag="VHS06_05470"
     CDS             5230..5757
                     /locus_tag="VHS06_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2987602.1"
                     /translation="MQRRYIVCDPGECTGCRLCEFACALEKTGSFDLELSRIRVASPQ
                     PTAAISIACQLCEGAPCVSACPRDALSVRPQDGTIGLEKALCTGCGWCIEACDFGAIA
                     MDPGTKSVVICDLCSTLPTPACVEACPKKALSLSPPSAVAGRSRRTAAEKLLKDVPVT
                     EADGRGRRLSAISYM"
     gene            complement(5748..6614)
                     /locus_tag="VHS06_05475"
     CDS             complement(5748..6614)
                     /locus_tag="VHS06_05475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX2987603.1"
                     /translation="MGSLEGKVALVTGASRGIGRAIAVELARSGARVAANYRSGEAEA
                     KALAEEIAAMKAAAVVAESRALAQARAIVDELSTRVGTVPEARTGDGNSAPTDTIMLV
                     RADVSASGEAREMIRKVVERWGRLDVLVNNAGITRDHVLRKMTDDEWQEVLNTNLNSV
                     FYTTTAAIPVMVEQKFGRIVNIASAIGQTGNIGQANYGAAKGGIIAFTKSAALEVARY
                     NVTVNCISPGFTATEMLLRVPENILEQIRAKIPMGRFAQPDEIAKAVTFLASDGDYIT
                     GQQINVNGGIYM"
     gene            complement(7033..7488)
                     /locus_tag="VHS06_05480"
     CDS             complement(7033..7488)
                     /locus_tag="VHS06_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987604.1"
                     /translation="MIATFETFDILLTEATRQAARNAEFANDTVRRYMDEVSTANSNQ
                     FAALDALQQALFKSVFDIQTASLQLSRTVGDAASTLNRAIARWALTAIFPGITLGSLT
                     EPARTEKRSWDRTPESDDEATRIGDVATSALRFQGPMSVISSGMGEYEG"
     gene            complement(7703..8059)
                     /locus_tag="VHS06_05485"
     CDS             complement(7703..8059)
                     /locus_tag="VHS06_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987605.1"
                     /translation="MASPKTVGQFYVETTRQTAKNAASAQDAIVRYLDELTGVGKAYL
                     SAWSSAQQLSLQTAFQLQDTAIQAAQAMLDASAQANLSLFDEWAKAVANGQVAMTKVV
                     ATGVSMLDNAMPRRIG"
     gene            complement(8075..8413)
                     /locus_tag="VHS06_05490"
     CDS             complement(8075..8413)
                     /locus_tag="VHS06_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987606.1"
                     /translation="MATVTETMESTTIEATKLAQDAARRYMDESAAIGRTYFNNWAAA
                     AQASLRIAFDVQNAMIQASRSILEATTQANRNWFDQGADSVRKGQDATTKMVAAGFDA
                     MGSAVPKSRG"
     gene            8580..>9079
                     /locus_tag="VHS06_05495"
     CDS             8580..>9079
                     /locus_tag="VHS06_05495"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2987607.1"
                     /translation="MAGKTARTREETGSRSSARPKQERRESAENPNQPAAENGTGEGL
                     FSWTFRDFLIPYILLALSMQRAHGYFIEQYLRGLGLVNVEMSTLYRTLRQLERDGLVY
                     STWEPGPEGPARRIYSLTDAGRWWLDAGAAALSGITLGSLTEPARTEKRSWDRTPESD
                     DEATRIG"
BASE COUNT         1632 a         2630 c         3086 g         1731 t
ORIGIN      
        1 agttgctgag ttccgagaag gactctcact cagaacccag aacccagaac tcagaacctg
       61 aggccgttcc gttgccgttg cggccgccgg tgttgtgccc ggggtgcccg cacacgggag
      121 ccttctacgc gctgaagagg ctcggcttct atcgcggtac cggtgaggtg tcgcagaacc
      181 tgcctggcca gatactggcg aagctgaagc ggtcgggcgt ggtgatctca ggggacattg
      241 gctgttacac tctggccgtt ctgccgccgc tcctggcgat ggacagctgc ggctgtatgg
      301 gagcaagcat cggcggggcg atggggatgg aaaaggcggg ccttgccaac aaggtggtgg
      361 cggtgatcgg agattccact ttcctccact ccgggatgac gccgctgttg gacgtggtgt
      421 acaacggcgg aaccattacc acgatcatac tcgacaactc caccaccgcc atgaccggcc
      481 accaggagca cccggggacg ggccggtccg tcacgggcgc ccatgcccgg aaggtcaacc
      541 ttgagacact ggtgagaggg ctgggcgtgg aggatgtgaa ggtggtgaac gccttcgcgg
      601 tggacgaggt ctcggctgcc atccgggata gcgtcgagcg ggacgagccg tcggtcgtca
      661 tcgtcaaggg ggagtgctcg ctgaaggcga aagtggcggg cgagccgttc gtggtggatc
      721 ccgcgctgtg tgatgggtgt ggcgcttgcc tgcgcactgg gtgccctgcc attgtcctgc
      781 gggacgagaa agcggagatt caggccggca tctgcgtggg catcgagtgc ggtgtgtgtg
      841 cacaggtgtg ccctaagggc gccattttgg caccaggagt cgccggacag aggacttcgg
      901 acaggggcat ggggcggagc gaacgtggat aaaatggact tcttgctggc gggcgtgggt
      961 gggcagggga ttgtgctgat gagcgacctc ctggccgagg ctgcactgga agcgggctac
     1021 gacgtaaaga agtccgacgt cttcgggatg gcacagcgcg gcggcagcgt caccagctac
     1081 gtgcggatcg gtgagaaggt gtactccccg ctacccaggc ctggcgaggt ggactttcta
     1141 gtggggttgg agaagttgga ggcggccagg tgggcgtctc acctggtgcc cggcggcacc
     1201 gccgtggtga acgactactc cctcttccct ctggaagtga gctcgggggc cgagcgatat
     1261 cccacggacc agcaggtgat tggcagcttc gagtcgcgcg ggtgccgggt gcatctggtg
     1321 gacggcaccg ggatggcgag gcagctcggc aatcccaagg tactgaatgt cgccgtgctg
     1381 gggcagctgg cttcgctgct tcctctgccc tgggaggcgt ggacgcggac ggtggagcgg
     1441 cgtatcccgg cccggtttcg ggaattgaat ctgagggcgc tggcgcttgg acaggaaacg
     1501 gcgagatcag aactgaggac cgcggactga ggactgagtc ccgtaacaag cactcagtcc
     1561 tgggcaggga tgcctacccc actacgacgg agtagcggat cacaagtgac tttggggtcg
     1621 cgaggcgacc gggccagacg cacaattcgc actcactgga ggtgggcatg tactgggatc
     1681 agcagcacga gacactgccg agagccgaga tggaagcact ccaggtggag cgactccggc
     1741 gcaaggttcg agaggtctac gagaaggtgc ccttctacag gaacgcgttc caggagaggg
     1801 gcatcacgcc tgaggatatc tcctcgctgg acgacctcac caagctccct ttcaccaaca
     1861 aggccgattt ccgtgacaac tacccctttg gtctgctgac gattccgctg aaggatgtgg
     1921 tcagggttca cggctctagt ggaactaccg gcaaacccac ggtcgtcgcg tacgcgcgtg
     1981 ccgacctgga actgtgggcc gacctcatgg cgaggggcat gaccatgggc gacgtcacgg
     2041 atgaagatat cgtccagaac gcctacgggt acgggctctt cacgggcggg cttggcttcc
     2101 attacggcgc cgagcgcatc ggagccacgg taatccccat atctggcggc aacaccaagc
     2161 gccagataat gcttatgcag gacatgaaga ccacggtcct cacttgcact ccctcctact
     2221 ccctcttcct tgcggagacc gcgcgcgaga tgggggtgga cgtgcgcgaa cttcccctgc
     2281 gggttggctt cttcggcgct gagccgtggt cataccggat gcgccaggag atcgaggagg
     2341 cgctggggat tctggccctg gacacctacg gcctgagcga gataataggc ccaggcgttg
     2401 cccaggagtg ccctcacaag cgcgggctgc acatctacga ggaccacttc ctacctgaga
     2461 tcgtggaccc tgttactggc gagtcggttc ccgacggcgt gaagggcgag ttggtgttca
     2521 cgacactgac gaaggtggcg ctgcctgtaa tccgataccg gacgcgggat atcaccagcc
     2581 tgcatcgcga gaaatgcgac tgcggcagga ccctggtacg tatggagaag gtgaccggac
     2641 gcacagacga tatgctgatc gtccggggca tcaacgtctt cccctcccag atcgagagcg
     2701 tgctgctgga ggtggatggc gtggatcccc actaccagat cgttgtagac agggcgcgtg
     2761 gtctcgacga cctggagatc caggtagagg tctcggagac ggtcttctcc gacgagatcg
     2821 ggcggttgga aagcctgacg aagaaggtcc gagtggagat cgagagcacg ctcggcatcc
     2881 aggcgctggt gaagttggtg gagccgggtg cgatccctcg aagcgagggc aaggcgaagc
     2941 gggtcatcga ccggcgagag ttgtgagaag ggggagagta cggccatgag gatgaagcag
     3001 gtttcggtat tcgccgagaa tcgggcggga cggctgaaga cgctactggc agtacttgcc
     3061 agggcagaga tcaacatccg cgcgctgtcg atcgctgaca ccgcggactt cggcatagtg
     3121 cggatgatcc tgtccgacac cgacgggggg cttgaggcca ttcgcgcggc tgggttcgct
     3181 gccagcacca acgatgtgct tcgtgtcgag gttccggacc agccgggtgg gctgctgcag
     3241 gccgtcgccg agccgctggc cgcggccggg gtcaacatag aatacgttta tgccttcgcg
     3301 gacaatcctg agaagcacgc gatggtggta ctgaaggtgg gagatatcga cgcggcagag
     3361 aggcttctca gcaagtgagc atgagcagct ttctggggaa gcttctggta gtcgacctct
     3421 cggcgagggc cgctcgtgag gtatccctgg atccagagtt gtgcagacgc tatttgggtg
     3481 gcataggtat ggccaccaag ctcctgtggg actactcgcg tccgcggacc gaccccttct
     3541 cgcccgagaa cccgctggtc tttgcggccg ggccccttgc tgggacgatg gcgccgtcgg
     3601 ggagcaaata cgcggtcgct accaagtcgc cactaactgg gtttatcggg gactcccttt
     3661 cctcgggagg gtggcccctg gcgctgaagc gagccggata tgatgcgatg gttgtgcagg
     3721 gtgcggcgga cgtgccaacc tacgtgttcg tggatgacga tcgcgtctac ttcaggtcgg
     3781 cgcggcgtct ttggggtggc aagtgctggg aaaccgagga agcgatccgg cgagagctcg
     3841 gggacgacgg ggttcgagtc gcgaccgtgg ggcccgccgg cgagcggctg gtccgatatg
     3901 cctgcatagg gaacgactat gggcgtcagg caggaagaac cggtcccggg gccgtcatgg
     3961 gttcaaagaa cctcaaagcg atcgctgtcc gtggcaccaa gcccgtcggg gtatccgacc
     4021 ccgggcgcgt gaaggcggcg agcctcgacc tgtatgcgcg ctctcaggga gcggccacca
     4081 ccaagtatcg cgtgctaggg acgccagcga acgtgctggc tctcgatgcg ctggcggcgt
     4141 tgccggccta caacttccgg cagtccacct tcgagaaggc gaaccgcgtg agtggagagt
     4201 acttctacga gagattcaag gcgaaggtgg tggcgtgcgc ggggtgtccc attggttgcg
     4261 atcacatcta cagggtgacg gacggccagt acgcggggac cgaggtcggg atggactatg
     4321 agtcgttgta cgcactgggt ccgctctgtg gcatagacga cgcggcagct atcttgaagg
     4381 cgtccgaact gtgtgatcag tacggactcg atactatgag caccggcgcc tccatcgcct
     4441 gggccatgga gagctacgag cgcgggttaa tctcccgaga tgaggccgag gggctcgatc
     4501 ttcgcttcgg gagcgccgag gcagtagtga ccatggtgga gcagattggc ctcagacggg
     4561 gcctaggcag gctactgggc gagggcgtca ggcgcgcagc ggctacgctt gggagcggat
     4621 cagacttctg ggcgatgcac gtcaaggggc tggagttacc gggctacgag ccgcgaagtc
     4681 tgaagactct ggctctcggc ctagccgtgg gaacccgtgg cgcctgccac aatcggtccg
     4741 cgggctacga ggtggacctt tccgctaagg tggacaggct ctcaagcgag cgtggccgcg
     4801 gcccgcttgc catggcgcag gaggactacg ctgcgacgct ggactccctt ggcgtctgca
     4861 aattcctgcg acgctgcttc gacgccttcc acgtagaggc ggcagacctg ttcagggcca
     4921 cgaccgggct ggagttgaca gcggaggagt tgcagaaggt cggagagcgc gtttcgaact
     4981 tgaagaaggc gtacaacatc cgggaggggt ggactcgagc ggacgacacg ctgcctccca
     5041 ggattctcga ggaaaagctg ccgaccggcg tggccagagg gaccggcctg acccgcagcg
     5101 agctcgacga gatgatcgac ggctactacg aggcgagggg ctggactacg gaggggctga
     5161 taccaccagc aaagctggcc gagcttggca tggaagaact cggggcggga gtgagtgagg
     5221 gcgcgagtga tgcaaagacg ctacatagta tgtgatcccg gcgagtgcac aggctgccgt
     5281 ctgtgtgagt tcgcctgtgc cctggagaag accgggagct tcgacctgga gctatccagg
     5341 attcgggtgg cctcacccca gcccactgct gccataagca tcgcctgcca actgtgcgaa
     5401 ggcgcccctt gcgtctcggc ctgtccgcgg gatgcgctct cggttcggcc gcaggatggc
     5461 acgataggcc tggagaaggc cctctgtact ggttgcggct ggtgtatcga agcttgcgac
     5521 ttcggggcga tcgccatgga tccggggacc aagtccgtcg tgatctgtga tctgtgcagt
     5581 acccttccta ctccggcgtg tgtcgaggca tgccccaaga aagccctcag cctgtccccg
     5641 ccctcggccg tcgccgggcg ctcccgcagg accgccgcgg agaagctgct caaagatgtg
     5701 ccagtaactg aagccgacgg ccgcggccgt cggctctcag ccatctctta catgtagatt
     5761 ccgccgttga cgttgatttg ctggccggtg atgtagtccc cgtcagacgc gaggaatgtc
     5821 actgccttgg cgatctcgtc gggctgagcg aaacggccca ttggtatctt cgctcgaatc
     5881 tgttccagga tgttctccgg gactctcaac agcatttcgg tggcggtgaa gccggggctg
     5941 atgcagttca ccgtcacgtt gtacctggct acctccagcg ctgcgctctt ggtgaaggcg
     6001 atgattccgc ccttcgcggc gccgtagttg gcctgcccga tgttaccggt ctgccctatg
     6061 gccgaggcga tgttcacgat gcgcccgaac ttctgctcca ccattacggg aatagcggcc
     6121 gtggtcgtgt agaagacgct gttgaggttc gtgttgagaa cctcctgcca ctcatcatcg
     6181 gtcattttcc gcagcacgtg gtcccttgtg atgccggcgt tgttgacgag gacatcaagc
     6241 ctgccccagc gctcgaccac cttacggatc atctccctcg cctcgccaga cgctgagacg
     6301 tctgctctca ccagcattat ggtgtcggta ggagccgagt tcccgtcgcc cgtgcgagcc
     6361 tctggcactg tcccgaccct ggtcgatagt tcgtccacga ttgcccgtgc ttgggccaga
     6421 gccctgctct cagccactac cgccgcagcc ttcattgccg cgatttcctc agccagcgcc
     6481 ttcgcttcgg cctccccgga tcggtagttc gctgctaccc gcgcgccgga gcgggccagt
     6541 tccaccgcga tggccctccc gatgcctctt gatgcgcccg tgaccagtgc caccttgcct
     6601 tccagtgagc ccatgtgaag cctccccctc tcctaggttg gtgtgagttg tgctcggata
     6661 tgtgcggtgg acgaacggac gaatgaggat gtcagtgccc ctactctgcg ggagtacccc
     6721 ccctttcggt aagacgattg aaccggactg ggaaagacat gtagagtcaa tgcggccaag
     6781 cttgttcgct tgcaaagttc atgccgcaac taccacttat cccgggaaat tcgtgcgctg
     6841 atttcttacg cctggagagg tagggcggac gacgggagga ggaaagtcgc gggggcgagg
     6901 tagggttctc cgaagcccag ccggggggcg cctcataagt gcttcttggg cgggtgctcc
     6961 ctcccgagca agtttccgtg gccgcggcag gcttctcggg ctgatcgcgg tgagggttcc
     7021 agagaattct gtttagccct cgtattcccc catcccggaa gagatgacgc tcatcggccc
     7081 ctggaatcgt agcgcgctcg tggccacgtc tccgatgcgg gttgcctcgt cgtcagattc
     7141 tggagttcga tcccaggagc gtttctcagt gcgagcaggc tctgtcagag atcccaaggt
     7201 tatccctggg aagatcgcgg ttaaggccca ccgcgcgatg gcgcggttaa gagtcgatgc
     7261 agcatcgcct actgttcgag aaagctgcag cgaggcggtc tggatatcga agaccgattt
     7321 gaaaagcgct tgctggaggg cgtcgagggc cgcgaactga ttgctgttgg cggttgacac
     7381 ctcatccatg tatcgacgta cggtgtcgtt ggcaaactcc gcgttcctgg cggcctgcct
     7441 ggttgcctcg gtaagcagaa tgtcgaaggt ctcgaatgtg gcgatcatgg cgctgtcgac
     7501 ctcctagcct ggccgataga tgatcgctac gtcgcccgcg tgggtagccg cgccttcatc
     7561 tgcccactcg gccagggcgc actgaatcgg cgactcaggt tcgagccgct ggacgcggtc
     7621 gggctggagg cggcagacac aggacgcctg ccgctctctc ccagcacgtc accgaaaacg
     7681 agccgctgct caccggtgtc ggctatccga tccggcgcgg catcgcgttg tcgagcatgc
     7741 tcacgcccgt ggcgaccacc ttagtcattg caacctggcc gttcgccacg gccttcgccc
     7801 actcatcaaa gagcgacaag tttgcctgag cagacgcgtc taacatggcc tgagctgcct
     7861 gaatcgccgt gtcctggagc tggaacgcag tctgcaagct gagttgctga gcgctgctcc
     7921 aggcggacag ataggccttg ccaactccag tcagctcgtc caagtagcgg acgatggcgt
     7981 cctgggcgga ggcggcgttc ttggctgtct ggcgcgtcgt ctcgacgtag aactgaccga
     8041 cggtcttggg gctggccatg ttagtcactc cttactagcc gcggctcttc ggcactgcgg
     8101 agcccatggc atcgaagcca gccgccacca ttttggtcgt ggcatcctgg cccttgcgga
     8161 cggagtcggc gccctgatcg aaccagttgc ggttggcctg agtggtggcc tccagaatgg
     8221 agcgggacgc ctggatcata gcgttctgga cgtcgaaggc gatgcggagg ctggcctggg
     8281 ccgcggcggc ccagttgttg aagtaggtgc gtccgatggc ggccgactcg tccatgtagc
     8341 ggcgggccgc atcctgggca agcttggtgg cctcgatggt ggtgctctcc atggtctcgg
     8401 taacggttgc cattgccgtg cttcctcctc ttgctggtgg gcggcctcgc gattcttgac
     8461 gggccgtgct agaatagctg cggatagatc acattgcagc tatatgcatt gtacacttag
     8521 gtggtgagat gtcaagcccc gattccaaaa ggcgtcgcgg atctcgagag gagcctccga
     8581 tggcgggaaa gacggccagg actcgcgaag aaaccgggtc ccgatcctcc gcacggccca
     8641 agcaggagcg tcgggaaagt gcggagaacc ctaaccagcc ggcagccgag aacggtaccg
     8701 gggagggtct cttcagttgg accttccgcg atttcctgat cccgtacatc ctgctcgcgc
     8761 tgtcgatgca gagggcgcac ggctacttca tcgagcagta tctccggggc ctggggctag
     8821 tgaacgtcga gatgagcacg ctctaccgca cgctccggca attggagcgg gatggtctgg
     8881 tgtactccac ttgggagccg ggtcccgagg ggccggctcg tcgcatctac agccttaccg
     8941 acgcagggcg ctggtggctc gacgcggggg cagctgccct ctcagggata accttgggat
     9001 ctctgacaga gcctgctcgc actgagaaac gctcctggga tcgaactcca gaatctgacg
     9061 acgaggcaac ccgcatcgg
//
LOCUS       DATALS010000244         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_102014, whole genome shotgun sequence.
ACCESSION   DATALS010000244 DATALS010000000
VERSION     DATALS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102014"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1638)
                     /locus_tag="VHS06_05500"
     CDS             complement(91..1638)
                     /locus_tag="VHS06_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX2987608.1"
                     /translation="MKIVTVQQMRTLEQASVQKGVSLDQLQLNAAAAVAKELEQLFPD
                     PERPALFLVGPGNNGRDALIAAGILMQTGWAVRVYLAPGVGSEDVLAVLRAGGAAIQR
                     HADAVGWATLQSLVAESEVVVDGLLGIGIHGSVREPMAGIVTVASAEAAANRVPVVAV
                     DLPSGIDADSGEVAGVALRADYTISLGCVKTGLLRFPAAEYVGTLIPVGIGLPAGIDS
                     DIRVELLTGADVAPLIPPRPLDGHKGTFGRVLLVAGSSNFVGASYLAGAAAARSGCGL
                     VTLAVPEWQRAALVPLLPEATYIPLLDGKEAQQGANLRAVMDALPACNALAIGPGLGQ
                     GDGIVELVLGVLEGNRVGPKVRTVVDADGLNALATVDRWWERIGAGNVVTPHPGEMSR
                     LVGIPTVEVNANRVDVALDAARRWGQVVVLKGAFTVVADPDGAAWINPAAMPALGSGG
                     TGDVLTGVLAGLMAQGASPLDAARAAVYLHTAAAELTLASNATDRLLASDLLTSIPRA
                     ICDLACL"
     gene            complement(1647..>2009)
                     /gene="acpS"
                     /locus_tag="VHS06_05505"
     CDS             complement(1647..>2009)
                     /gene="acpS"
                     /locus_tag="VHS06_05505"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX2987609.1"
                     /translation="RGMVLETGIDIIEIRRIEEAISRHGARFLDRVYTQGEIRQCRGK
                     AESLAARFAAKEAAFKALGERVGWREVEIQRERSGKPRVLLHGRAAEVADRLGLRSWS
                     VSLSHSREDAVAMVVAGK"
BASE COUNT          381 a          759 c          543 g          326 t
ORIGIN      
        1 ggagaccgga gaggctcccc agtctgaacc ttccctcgcc gacgagatcc cacccaggac
       61 cgagcacccc acggagcccg agcaggaagc ttagagacag gccaggtcgc agatcgccct
      121 ggggatcgac gtcagcagat cgcttgccag caaccggtcg gtcgcgttgc tggcaagtgt
      181 caattcagct gcagcagtgt ggagatacac ggccgctcgc gcagcgtcca atggcgaagc
      241 cccctgagcc atcagtccgg ccagcacccc cgtcaacacg tccccggtgc caccactgcc
      301 caatgccggc atcgcggcgg ggttaatcca cgctgccccg tcggggtcgg ccaccacagt
      361 gaacgcaccc ttcagcacca ctacctgccc ccatcgccgc gcggcatcca gcgccacgtc
      421 caccctgttg gcattgacct ccaccgtcgg tatccccacc aacctggaca tctcgcccgg
      481 gtgaggggtg acgacgttgc cagcaccgat ccgctcccac catcggtcca cggtcgccag
      541 cgcgttcaat ccatcggcgt ccacgacggt acgaaccttc gggccaaccc ggttaccctc
      601 caggacgccc agaaccaact ccacaatccc gtcgccctgg cccagccccg gaccgatagc
      661 caacgcgttg catgcgggca gggcatccat cacagcccgg aggttagccc cctgctgagc
      721 ctctttcccg tccagcaggg gaatataggt cgcctccggc aacagcggca ccagcgctgc
      781 tctctgccac tcgggcacgg ccagggtcac caacccacag cccgatcttg cggccgcggc
      841 acctgcaagg taagacgcgc ccacaaagtt gctggaaccc gccaccagta gaacacgtcc
      901 gaatgtgccc ttgtggccgt cgagtggtct gggcgggata agaggtgcaa cgtccgcccc
      961 tgtcagcaac tcaactcgaa tgtcggaatc gattcccgca ggaagtccga tgcccaccgg
     1021 gatcaacgtg cccacatact ccgcggcggg aaacctcagc aggccggtct tcacgcatcc
     1081 gagggaaatg gtgtagtcgg ctcgtagggc aacgccggcc acctcgccgc tgtcggcgtc
     1141 gatgccgctg ggaaggtcga cggccacgac cggcactcga ttcgccgcgg cttccgcgct
     1201 ggcgacggtc acgatccccg ccatcggctc cctgaccgat ccatgtatcc caatccccag
     1261 caggccgtca accactactt cggactcggc taccaaactc tgcagggtgg cccacccaac
     1321 agcatcggca tgccgctgga ttgcagctcc gcccgcccgc agcaccgcca gtacatcctc
     1381 agagccgaca cccggtgcca aatacactct caccgcccat cccgtctgca tgaggatgcc
     1441 ggcggcaatg agggcgtcgc ggccgttgtt gccgggcccg accaaaaaga gcgcgggcct
     1501 ctccgggtca gggaacagct gctcaagctc cttcgccact gcagcagccg cgttgagctg
     1561 aagctgatcc agggagacac ccttctggac cgacgcttgc tcgagtgtcc tcatctgctg
     1621 cacggtcacg atcttcatct ctgcacctac ttcccggcga ctaccatcgc cacggcatct
     1681 tcccgactgt gagagaggct tacagaccaa ctcctaagcc ccagcctatc cgcgacctcg
     1741 gcggcgcgac cgtgcagcag tacacgaggc ttcccactac gctcccgctg tatctcgacc
     1801 tcgcgccacc caactctttc accgagcgcc ttgaaggcag cttccttcgc cgcaaagcgg
     1861 gcagccagcg actccgcctt cccccggcat tgccggatct ccccctgcgt gtagacgcga
     1921 tcgagaaacc gagcgccgtg cctggagata gcctcttcta tccgccgtat ctcgattata
     1981 tctatgccgg tttccagtac catgcccct
//
LOCUS       DATALS010000245         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_382475, whole genome shotgun sequence.
ACCESSION   DATALS010000245 DATALS010000000
VERSION     DATALS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382475"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VHS06_05510"
     CDS             complement(<1..477)
                     /locus_tag="VHS06_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX2987610.1"
                     /translation="MAEPFLSEIRLFSFNFPPKGWAFCNGQLLPINQNQALFSLLGTT
                     YGGNGQTTFALPNLQGRVPMNAGPGHSLGESGGEQAHTLTINETPAHNHGAIAATSEE
                     ASSNQLAANCWAASDAVAYSSSAGGAGMGLGGAGTSAFGGSQAHMNLPPYLTINFCI"
     gene            complement(558..1058)
                     /locus_tag="VHS06_05515"
     CDS             complement(558..1058)
                     /locus_tag="VHS06_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX2987611.1"
                     /translation="MGQPYVGEIRMFAGNFPPVGWMFCEGQLLSISEYETLFNLIGTT
                     YGGDGQSTFGLPDLRGRLPIHQGAGYTLAQTGGVETVTLTPSQIQAHSHPVSCVPGPG
                     NQRNPIGNVWAGSATGEKPYSSELGNVALQPLAASGGSQPHNNLPPYLCVSFIICLFG
                     IYPSQV"
     gene            complement(1084..1590)
                     /locus_tag="VHS06_05520"
     CDS             complement(1084..1590)
                     /locus_tag="VHS06_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987612.1"
                     /translation="MTQSNEDVAARKRPRAITRREFGRRVGILIAGCGVLSAAQKVGI
                     AAASPASHTLSRPLDLLATLDRSAFSALAGQSFLVRSGAPVPVQMQLVEVAEVRQPTA
                     SAGQPGGAVPSTECFSLRFRGPSANPLSQNSYLFEHKVGSFQLFIVPSAPSADEQQYV
                     AIVNRLIS"
     gene            1696..2028
                     /locus_tag="VHS06_05525"
     CDS             1696..2028
                     /locus_tag="VHS06_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987613.1"
                     /translation="MLEDFTYATFAEQLGSRFRVGLDETKVVELELVEAIELSPSSAV
                     SGDAGAPKSRGESFSLRFLGSRQQFIPQGTYPFGHDEMGDFPMFITPVESTPAGIKYE
                     AVFNRLPG"
     gene            complement(2009..2434)
                     /locus_tag="VHS06_05530"
     CDS             complement(2009..2434)
                     /locus_tag="VHS06_05530"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2987614.1"
                     /translation="MESPSPCGFSVPASSSSTREAFLTSQYAAQRHHYLTHYSNASFQ
                     VILQGGQPAGRLYVARWEHEIRIMDVAVLPEHRNAGIGSALIKALQEEAGRDKKSVTI
                     HVEMFNRAARLYERLGFCNKALNGVYWLMEWTPPSQATG"
BASE COUNT          429 a          763 c          811 g          507 t
ORIGIN      
        1 gatgcagaag ttgatggtga gatatggcgg gaggttcata tgggcttggc taccgccgaa
       61 ggccgaggtg ccggcgccgc ctagccccat tccggcgccc ccggcgctgc tggagtaggc
      121 cacggcatcg cttgccgccc agcagttggc cgcgagttga ttcgagctgg cctcctcgga
      181 cgtggccgcg atggccccgt ggttgtgcgc cggggtttcg ttgatggtca gcgtgtgggc
      241 ttgctcaccc ccgctctcac ccagggagtg tcctggacct gcgttcatgg gaacccgacc
      301 ctggaggttc ggcagggcga aggtcgtctg cccgttgccg ccgtaggtgg tgcccagcag
      361 ggagaagagg gcctggtttt gatttatcgg cagcaactgc ccattgcaga aggcccagcc
      421 cttcggcggg aagttgaatg agaagagcct gatctcggac aggaacggct cagccatgtc
      481 tcgtttctcc ttgtcttggt tcgggactcg ccgaatgtgt catgcgagcg gcaatcggcc
      541 caccgggccg gacgcctcta aacctgtgac gggtatatcc cgaacaggca tatgatgaag
      601 ctgacgcaca ggtatggcgg caggttgtta tggggttggg accctccgct ggccgccagg
      661 ggttgcagcg cgacgtttcc cagttcgctc gaatagggct tttccccggt ggcggagcct
      721 gcccacacgt tgcctatcgg gtttcgctga ttccccggcc ccgggacgca gctcacggga
      781 tgggagtgag cctgtatttg gctgggggtc agggtgacag tctcaacccc accggtctgg
      841 gcaagggtgt aaccggcgcc ctggtgaatc ggcaggcgac cgcggaggtc tggcagcccg
      901 aaggtactct ggccgtcacc cccgtaggtc gttccgatga gattgaagag ggtctcgtac
      961 tcggatatgg acagcaattg gccctcgcag aacatccagc ccacgggtgg gaagttgccg
     1021 gcgaacatcc tgatctcgcc tacgtatggt tgtcccattg gagcctcctt ctggtcgtat
     1081 tccctaggag atcaaccgat tgacgatcgc cacgtactgc tgctcgtcgg cgctgggcgc
     1141 cgagggcacg atgaacagtt gaaagctccc gaccttgtgc tcgaagagat agctgttttg
     1201 cgacagcggg ttggccgatg ggccgcggaa acggagcgag aagcactccg tcgatgggac
     1261 tgccccgccc ggttgccccg ctgacgccgt gggttgtctc acttcagcga cctcgaccag
     1321 ttgcatctgt accgggaccg gagcccccga ccggaccagg aagctctgcc cggcgagagc
     1381 cgagaaggcc gacctgtcca gcgttgcgag tagatccagt ggtctggaga gggtgtgcga
     1441 tgcaggggac gcggcggcga tgcccacctt ctgggcggcg gacagaacgc cgcagccggc
     1501 gataaggata cctactcgcc ttccgaactc gcggcgagtg atcgctctcg gccgcttccg
     1561 cgcggccacg tcttcgttgg actgtgtcac ctggcacctc cttcatcgtc ggtagccggt
     1621 tgccccggat cgacggcgat gggataatcc gcgacgaacc gccgatccgc tatccttttg
     1681 caggggttac ttgccatgtt ggaagacttc acatacgcga ccttcgccga gcagttgggc
     1741 tcgcgttttc gtgtcggact tgacgagacg aaggtagtcg aactggaact cgtcgaggcg
     1801 atagagctat ctccatcctc ggccgtgtca ggtgatgccg gagccccgaa gagccgtgga
     1861 gagtccttct ccctgcggtt tctcggttcc cgccagcagt tcatccccca gggaacgtac
     1921 cccttcggac acgatgagat gggggatttc ccaatgttca tcacacccgt cgagtcgacc
     1981 cctgccggca tcaagtacga ggccgtgttc aaccggttgc ctggctaggc ggtgtccact
     2041 ccatcagcca gtagacacca ttgagagcct tgttgcagaa ccccagccgc tcgtataggc
     2101 gagcggcccg gttgaacatc tccacgtgga tggtcaccga cttcttgtcc cggcccgcct
     2161 cttcctgcaa cgccttgatg agggcgctgc ctatccccgc attccgatgt tccggcagca
     2221 ccgcgacgtc catgatgcgg atctcgtgtt cccacctggc cacgtacagc cgccccgccg
     2281 gttgtccgcc ctgcaagatg acctgaaagc tcgcgttgct gtagtgggtg aggtagtggt
     2341 ggcgctgtgc agcgtactgt gaggtgagaa acgcttccct ggtggatgaa ctgctggcgg
     2401 gaaccgagaa accgcaggga gaaggactct ccacggctct tcggggctcc ggcatcacct
     2461 gacacggccg aggatggaga tagctctatc gcctcgacga gttccagttc
//
LOCUS       DATALS010000246         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_25835,
            whole genome shotgun sequence.
ACCESSION   DATALS010000246 DATALS010000000
VERSION     DATALS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25835"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VHS06_05535"
     CDS             <1..222
                     /locus_tag="VHS06_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2987615.1"
                     /translation="YLRTTNPIESTFAGVRLRTDTTKGCGTRSACLAMVFKLCELAQK
                     HWRKLNGSAWLPQLLNRAIFEDGELKQAA"
     gene            442..1236
                     /locus_tag="VHS06_05540"
     CDS             442..1236
                     /locus_tag="VHS06_05540"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX2987616.1"
                     /translation="MSQPSSIEPRIGLDMRGRPTHGHLPHLPLCLNGHGPFWFLFDTG
                     AQGCDISPRVIKALGVEATDGSVPLSELRIGSLVWNDLRMGTGDTQRIEQVLDHPIDG
                     MLGGALMSWGKLAIRIDYPCSQSALWRSDGQPLPDAITIQIKDHYLLVPVHVNETGPH
                     LFLLDTGSSFCAICPELAQTLGLPLIRPTSTRGVLSILPGHDSIASSIRLAGVEARDL
                     DIIVEPFSLTERRVGAPIVGRIGHSFLRHCTMTLDYPQQLLSLHRP"
BASE COUNT          413 a          732 c          618 g          431 t
ORIGIN      
        1 tacctgcgga cgaccaatcc gatcgagagc acctttgccg gggtgcggct gcggacggac
       61 acgaccaagg gctgcgggac gagaagtgcc tgcctcgcga tggtgttcaa gctctgcgag
      121 ttagcccaga agcactggcg gaaactcaac ggcagcgcgt ggctgccgca actgctcaac
      181 agagcgatct tcgaagacgg agaactgaaa caggccgctt aggctccacc cgatcccatc
      241 cacaacattt gacagtacct ctcctcccca gcgccccagc ggcttcccga ggggtacgat
      301 gggggttgcg gggggtgggt tggggctgtt gcgggcggac acaataccag caggttggcg
      361 tcgccatcgc ctgatggcca ggcaagctgt cccgatgata cgagccgtgg agcacggcct
      421 gcggtatggt actatgttgc gatgtctcag cccagctcta tagagccccg catcggcctc
      481 gacatgcgcg gccgccccac gcacggccat ctcccccacc ttccgctctg cctcaacggg
      541 cacggaccct tctggttcct gtttgacacc ggtgctcaag gctgtgacat ctcgccccgc
      601 gtcatcaagg ccttgggcgt ggaggccacc gacggctcgg tgccactgag cgaactcagg
      661 atcgggtccc tcgtctggaa cgacctgcga atgggcactg gcgataccca gcggatcgag
      721 caggttctcg accatcccat cgacggcatg cttggcggag cccttatgag ttggggcaag
      781 ctggcgatcc gaatcgacta cccgtgctcg cagagtgccc tttggcgcag tgacggccag
      841 ccgctccccg atgccattac gatccagatc aaagaccact acctcctggt tcccgtgcac
      901 gtgaacgaaa ccggccccca tctcttcctg ctcgatacgg gatcctcctt ttgtgccatc
      961 tgcccggaac tggcccagac cctcggcctg cccctgatcc gtcccacctc cacgcgaggt
     1021 gtcctgagca tccttcccgg ccacgattcg atcgcctcct cgatccgcct ggctggcgtc
     1081 gaggcccggg accttgacat cattgtcgag ccgttctcac tcaccgagcg acgggtcggt
     1141 gccccgatcg tggggaggat cggccattcc ttcctgcgcc actgcaccat gaccttggac
     1201 tacccgcaac aactcctatc cctgcatcgc ccatagagtt cccgatcacc atccccaacc
     1261 tgcgccacca tggtcctccc ctcagggggc agtaccccgt actatgttca atgctttaca
     1321 acgttagcag gttacgtccg ctcggctcgg gagcacgatt ccgcatttga ggggatcgat
     1381 atgccgcact cagggcagcg gtccttgctt gctcggagat cgtagccgca ttttgagcag
     1441 tgaccatgtt tgtgacgctt tcgctccgtc cacgcattcc aagagtacgt tgcaggtaga
     1501 acggctgcta ccagcatggg cagccaatag gggattctga ccatccagta agacgggcca
     1561 taccaataac cgaacccaaa ccgttggtta gtacgtgccg aggagacgaa gtctcccgcc
     1621 gccatgctct catagttgag ccctgccgtg tatctctggc taacggacgg aatcggcttg
     1681 tcattggcga ccaggtcaat tgaaccccaa tccgaactca gaccacagag atgagggggc
     1741 gattgatgga cactgcagta ggtgattccc tccaaaatcc agtagcttcg cacccacaac
     1801 acgcccatcg ctacgcacag cagcagcgac agggccgcga ggatgttgaa gaggcggcgt
     1861 ttcattggcc cggtgattct accatgccct gactccgcat cgagccttct cgttcttccc
     1921 ggcggctgcc cagcgttgcc ccgaggggca tgacgggggt gcgggagtgg gcaggtcccc
     1981 agatcacccg catgaaccgc tgcaggtcag catgatccgc ctctccgcac cttgattgcc
     2041 atcgtgtgct tctatggcaa ggatcggccc gccaacgctt cgccgatccc gccgccgagt
     2101 tgtggcggct ctggcggctc gatactcacg ccgagttcct ttagcaccaa aatacggcgt
     2161 tgctcaagcg tcagatgccc cttggcctta acct
//
LOCUS       DATALS010000247         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_418368, whole genome shotgun sequence.
ACCESSION   DATALS010000247 DATALS010000000
VERSION     DATALS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418368"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VHS06_05545"
     CDS             <1..487
                     /locus_tag="VHS06_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2987617.1"
                     /translation="FDDLTNERFVPICIESSAGVDRTLLTFLVDSYENDVANERVVLH
                     LHPRLAPVKVAVFPLSKKADLAEKAHALKAELKQEWVTAYDDAGSIGKRYRRQDEIGT
                     PFCVTVDFDSLEDGKATVRDRDAMTQDRVDLSQIRAYLRERLYPKETPAAPIPSGPTK
                     E"
     gene            499..1413
                     /gene="hslO"
                     /locus_tag="VHS06_05550"
     CDS             499..1413
                     /gene="hslO"
                     /locus_tag="VHS06_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018759454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX2987618.1"
                     /translation="MAQEDYLVRTMAADGQIRAFCCRTTNLSEEAHLRHGTLPTATAA
                     LGRVLTGAAMLGALLKREERVTLQLIGDGPLGSLVGEADAHGHVRGYVQNPEVHLPST
                     PAGKLDVAGAVGHNGNLYVLRDLGLKEPYRGSVSLVSGEIAEDLTYYFVRSEQTPSAV
                     ALGVLVETTNSVRAAGGYLLQLMPGADPHLGRELEEIVRRTAPASTLIDQGQTPEAIL
                     SDLLGSLGVGEMTHQPLVYQCRCSRERLSRALVTLGPTELSELAGEPAGAELICRFCG
                     AKYQFSPDDLRALAEATAQESGTELGTH"
BASE COUNT          267 a          470 c          517 g          278 t
ORIGIN      
        1 tttcgacgat ctgaccaacg agcggttcgt ccccatctgt atcgagtcct cggccggagt
       61 ggaccggacg ctgcttacct tcctggtgga ctcctatgag aacgacgtgg ccaacgaacg
      121 ggtcgttctg caccttcatc cgcgcttggc tccggtgaag gtggcagtct ttccgctttc
      181 gaagaaggcc gatctcgcag agaaggcgca cgccctcaag gccgagttga agcaggagtg
      241 ggtgacggcg tacgacgacg ctggttccat cgggaagcgg tatcgtcgcc aggacgagat
      301 tgggacgccg ttctgcgtga cggtggactt cgattcgctg gaagatggga aggcgaccgt
      361 acgcgaccgc gacgccatga cccaggaccg tgtggacctg agccagattc gagcctatct
      421 ccgggagcgg ctctatccca aggagacgcc cgctgccccg attccctcgg gcccgaccaa
      481 agaatgaggg ggacgaccgt ggcccaagaa gattatctcg tgaggacgat ggccgcagat
      541 ggtcagatcc gggctttctg ctgccgtacc acgaacctgt ccgaggaggc gcacctgcgc
      601 cacggcaccc tgcctacggc caccgcagcc ctgggacgtg tgctcacggg tgcggcgatg
      661 ctgggcgccc tgctcaaacg ggaagaacgg gtcacattgc agctcatcgg cgacgggccg
      721 ctcggctcgc tggtcggtga ggcggatgcc cacggccacg tccgcggcta tgtacagaac
      781 cctgaggtcc atctgccttc tacgccggcg gggaagctgg acgtagccgg ggcggtaggg
      841 cacaacggaa acctgtatgt cctgcgtgac ctcggactga aggaacccta ccggggttcg
      901 gtgtctctgg tgagtgggga gattgccgag gacctgacct actactttgt ccggtcggag
      961 cagaccccct cagcagtagc ccttggggtt ctggtcgaga ccaccaactc ggtgagggcg
     1021 gccggaggtt acctccttca gctgatgccg ggggcggatc cccatcttgg gcgggaactg
     1081 gaggagatcg tgcgccggac ggcaccggcc agcaccctga tcgatcaagg ccagactccc
     1141 gaagcgatcc tctctgatct gctcggatcc ctaggggtgg gggagatgac ccaccagcct
     1201 ctggtttacc agtgccggtg cagccgggag cggctcagcc gggccttggt gaccctcggc
     1261 cccaccgaac tctccgagct ggccggtgag ccagccgggg cggagttgat ctgccgcttc
     1321 tgcggcgcca agtaccagtt ctctccggat gacctgcggg ccttggccga agccacggcc
     1381 caggagtcgg ggacggaact cggcactcac tgaactacgg aggggggagc ggcatgggtc
     1441 gccgttcgtt gacgctgctc atcctggtgg tattgctcgg cagcggcttg atcacgggat
     1501 tagcctactt ctacacggac tggctgtggt tc
//
LOCUS       DATALS010000248         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_207764, whole genome shotgun sequence.
ACCESSION   DATALS010000248 DATALS010000000
VERSION     DATALS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207764"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VHS06_05555"
     CDS             <1..531
                     /locus_tag="VHS06_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987619.1"
                     /translation="RRGCGLGLSIVQAVVEDHHGYIDLESEIGKGTTFTVYLPIHRAP
                     DEEASADGLQGGTETILVVDDDQIQRGVSTALLETLGYQVRTASSGEEAVEYLRSHSV
                     DLLMLDMIIEDKIDGTETYRRVLEVRPGQRAIVVSGYAESDRVREALALGVGAYLRKP
                     LTIVDLARAVRAELDR"
     gene            complement(642..>1590)
                     /locus_tag="VHS06_05560"
     CDS             complement(642..>1590)
                     /locus_tag="VHS06_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX2987620.1"
                     /translation="DTYRPAAINQLITLGKQIDMPVFSDERAKPPQICESAVKHAKNT
                     GLTAVLLDTAGRLHVDDSMMGELQEIQRRTSPTETLLVADAMTGQDAVRVAEEFNSKV
                     KLTGLILTKMDGDARGGAALSMRAVTGVPIKFVGVGEKLDALEPFFPERMASRILGMG
                     DVLSLIEKAQENYDQAQAQKLEKKLKAGQLDLEDFLEQMQQLKNMGPLSQVLDMIPGL
                     NKLARTPEAAKALDGNQMKRVEAIVLSMTSEERRNPQMIGGSRKRRIARGSGTTPADV
                     NRLLNQFFEMQKMMKAMASGKGPFGRGKMPPGLPGGFGL"
BASE COUNT          280 a          489 c          500 g          321 t
ORIGIN      
        1 aggcgaggtt gcgggctggg tttgagcatc gtccaggcgg tcgtcgaaga ccatcacggt
       61 tacatcgacc tggagagcga gatcgggaaa gggaccacct tcaccgtcta cctgcccatc
      121 catcgagcac ccgacgagga agcgtcggcg gatgggttgc agggtggcac agaaaccata
      181 ctggtagtcg atgacgatca gattcagcga ggggtgtcga cggcgctgct cgagacgctg
      241 ggctatcagg tgcgaacggc gtcgagtgga gaggaagcag ttgagtatct caggagccat
      301 tccgtcgatc tcttgatgct ggacatgatc atagaagaca agatcgacgg gacggagacc
      361 tatcgccgcg tgctagaggt gaggcccggc cagcgtgcaa tcgtcgtgtc gggttatgcc
      421 gagtcggatc gggtgcgcga ggccctggcg ctgggtgtgg gggcttacct gcgcaaaccg
      481 ctgactatcg tggatctggc aagagcggta cgggcggaac tggaccgata agatcgccac
      541 gcgtccgaca cgacggttgc cgctcttgcg gctgtcaaac ggagccgagg cacagccatt
      601 agggcgcgga accgatcggt caggttccgc gccccaacag tctagagacc gaagccgccg
      661 ggcaggccgg ggggcatctt gcccctcccg aaaggcccct tgcccgaggc catcgccttc
      721 atcatcttct gcatttcgaa gaattggttc agcaaccggt tcacgtcggc cggcgtcgtt
      781 ccgctccccc gggctatccg tcgcttccgg ctacccccga tcatctgcgg gttgcgtcgc
      841 tcctctgagg tcatggagag gacgatagcc tccacccgct tcatctggtt cccatccagt
      901 gccttggctg cctcgggagt gcgggcgagt ttgttcaggc cggggatcat atcgagtacc
      961 tgggagagcg gacccatgtt cttcagctgt tgcatctgct ccaggaagtc ttccaggtcc
     1021 aactgaccgg ccttgagctt cttttccagc ttttgtgcct gagcctggtc gtaattctcc
     1081 tgcgccttct cgatgaggct cagcacgtcg cccattccca ggatccggga ggccatccgt
     1141 tcggggaaga acggctccag cgcgtccagc ttctccccga cacctacgaa cttgatcggc
     1201 acgccggtta cggcccgcat ggagagtgcg gcgccaccac gggcatcgcc atccatcttc
     1261 gtgagtatga gcccggtcag cttcaccttg gagttgaact cctcggcgac cctcaccgca
     1321 tcctggccgg tcatcgcgtc ggccaccagc aacgtctcgg tcgggctggt gcgccgctgg
     1381 atctcctgaa gctcacccat catggagtcg tccacgtgga gacgccctgc cgtgtccagc
     1441 aagaccgccg tgaggccggt attcttggca tgcttgacgg cggactcgca gatctgcggc
     1501 ggcttcgccc tctcatcgct gaacaccggc atgtctatct gcttgccgag ggtgatcaac
     1561 tgattgatgg cagccggtcg gtaggtatcc
//
LOCUS       DATALS010000249         8727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_249897, whole genome shotgun sequence.
ACCESSION   DATALS010000249 DATALS010000000
VERSION     DATALS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249897"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..823
                     /locus_tag="VHS06_05565"
     CDS             323..823
                     /locus_tag="VHS06_05565"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX2987621.1"
                     /translation="MPVQNPRDLFIYDLSTVLAAEQSISQQLSQMEQACQNPQVKQMF
                     SQHITDTQRQVSRVQDCFRLIGAQPMNVSCYAVDCMQQDMQQFVQQGPSQDMIDMFCL
                     GAALKTEHYEIAAYRGLVEKARMMGETQLVSVLEDNLREEIDTCKQVRQMGTQMGSQV
                     IHRMQP"
     gene            complement(820..1041)
                     /locus_tag="VHS06_05570"
     CDS             complement(820..1041)
                     /locus_tag="VHS06_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987622.1"
                     /translation="MNTAPDTIAAETEGADRPVALIALLAIREWLGDRLGVIAMEVDE
                     RSNPREMTTGPTFFLSADRPLSRCCPDSC"
     gene            1325..2494
                     /locus_tag="VHS06_05575"
     CDS             1325..2494
                     /locus_tag="VHS06_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX2987623.1"
                     /translation="MSFDENGKGKERVIATLERNWKAEMEGAATYRELASMENDKRRQ
                     SLLIKLAEAEERHAKKWSDQIVQLGGEDPSKREIAIPASRGVILTAKAAGLETALRRA
                     ESTEEEHVRQYESQARELGDEGIAAVLHELAEDESSHATTLRAMTGPAQQVQSRLESI
                     LKREKWHGKGGGWIGDAIYGVNDGLTAVFGIVSGVAAASSNNEFIVVAGLAGVLSSAL
                     SMGASAYLANKAEREVYEAELARESREIEENPEEEREELELFYQLKGFSEEESRTLVE
                     RMAEKPEQFLKALAHEELGLSEERLPNPNLAAVVGSVSTGLGGIVPVIPFFFLAGMTG
                     IIVSLVVSIIAHFAVGASKSIVTLRSWWKSGLEMTGVALLVAVVAYAVGNALAIH"
     gene            complement(2947..5292)
                     /locus_tag="VHS06_05580"
     CDS             complement(2947..5292)
                     /locus_tag="VHS06_05580"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX2987624.1"
                     /translation="MDLIFLCIGWLFGLCLGQAAVTGTQANDVLAVDPWLLGPWLGAL
                     AACALGAALLAGSDVRLRITALSLTTALLGIWRAFSVVPEADTLLERTGRVSIQGTVS
                     NRPEAQDNSLLLRLDADRLQPEGVGEWMGTRGRLLVRTSRYQEWGYGDRLVVRGELRA
                     VQPVSGYWADYLARQGIHTTLEYPTIGLLERASPDDPMTLIEAVRTRLDELCVELLPE
                     PHASLLSGILVGSRSRMPVEFRDALNVTSTSHVVAVSGFNVTVVAGIAQLLALRLLAR
                     REATLLAIVAVWVYSLLTGLPPSALRAALMATMGLLAILVGRGGDALSFLLFSGAVMV
                     GLDPPLLYDLGFQLSFLATTGLVLLEPVLRTCLSKLPGWLASSLSVTLAAQLATMPVL
                     VSSFHTLSLVSPLTNLLIAPMLPGIMAVGTVVVAAGAVAKPLGEFLAPVAWLYLTYLV
                     AVILWTARLPAASVQLGAPAAFYVAAYWLSLLGVALWPLPELRSFRERLLAAVSCVPR
                     WLLAGGAVTLISLMVLAISGRPAGRLPVYFLELGGGESTLIKGPAGHTVLVDGGGSPS
                     ALAGALGRYQGIGDSGLDAVVLTGYGRNRLDGLIEVAHRRRVDMVVQPGHPPSSAAGR
                     AWAELLEERQIPTFEPLAGQRILLGDDVWLEVDAVSSVGNGAEQPTLSMRLLSGSTSL
                     LFPGDIPRSVQEEIAVAWPQRASLLRVPGQGASGALEERFAQTVSPTVAVLSGKAGER
                     FAHTSEEVADLLRGATILRTDRVGTVEVVVGKEGYEVYTER"
     gene            complement(5329..5955)
                     /locus_tag="VHS06_05585"
     CDS             complement(5329..5955)
                     /locus_tag="VHS06_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEX2987625.1"
                     /translation="MVEWLERNRSNLATVVVVLVAVAAFTLLQLPRRPALQLISPAAT
                     PLPASIKVHVTGAVAGPGVYSLPVDARVEDALKAAGGPTDTAETAFLNLATPLKDGQQ
                     VVIPAAGADSQASSEQPVQTAGAGEKPLPVGIAVASSSTPVTSKLDLNSATRAQLEDL
                     PGIGTVLSQRILDYRAKNGRFSSVEELKEAKLVNASTFEKIRGLVEVR"
     gene            complement(6005..7138)
                     /locus_tag="VHS06_05590"
     CDS             complement(6005..7138)
                     /locus_tag="VHS06_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2987626.1"
                     /translation="MGILPRHLYSDTVGKAMTNSQYNLHPVGTGPFKLTRIGGDGITL
                     EPHPEYYGPVPHLAQLSFRFYPDYSGALAALEKGDVDAFANIDSSDVARLKANEKLAL
                     YSSPDYLRYTVLFINNTSPLFQDKSVRQAIAYAINRDGLIRTVMDGQGSPGKGPISPG
                     SWAFDTKAKSYEYEPKKAESLLDAVGWRDSNGDGIRDRDGQPLTFVILTNDNRRRVKA
                     GEMIAEDLRKVGFKCELQALGWSDLLKEYMAKRTFQGVIAEQWLMTADPDVYTLWHSS
                     QVGNGGFNFSGLANEKIDKLLEDAGRTIDRSIRTQQYAEFQQLWEDESPAVILYYPQF
                     NWAVSRTIKDVKLSAFIDSTSRFRHVSEWYTKTKSVTPTPDKK"
     gene            complement(7797..8021)
                     /gene="secG"
                     /locus_tag="VHS06_05595"
     CDS             complement(7797..8021)
                     /gene="secG"
                     /locus_tag="VHS06_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX2987627.1"
                     /translation="MQTYLNLVQILISIVLILVVLLQAKGSGFAGALGGGDSSVYRSR
                     RGVERTLYNFTIGLAVAFVLLSIVSVKFFS"
     gene            8195..>8727
                     /locus_tag="VHS06_05600"
     CDS             8195..>8727
                     /locus_tag="VHS06_05600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX2987628.1"
                     /translation="MAEVEIGILTRGKATLGMTLCSLLLQQVHDIRIFIVDTSEKPVI
                     QRDDVAFALRLAFDRQIQCNYEYLRDRERAFSVGRLKILEALKGRNLCFMDDDIVLPS
                     SSLARLLEDTIPESKYGFVAPYCRHSAFPPGFASDTPLYAPGTLFCQDAMVREILLDY
                     YRTTVDVLDRAPSPNKV"
BASE COUNT         1858 a         2722 c         2642 g         1505 t
ORIGIN      
        1 ctccaccgct cccctcttcg gcgtccccat gtccctactg acaggagaga agctgacatg
       61 gcagatcgtg ctcggcagca tcatatccgt cgcgggcatc tggatggtgg tcgcaggctg
      121 acttgatcgg cacggttcct gcgggctctt caacaccgga tgatcaccaa cgatgaaccg
      181 atcgatgcca ccgtcttgac atcacatgtc ggctcatgcc gccggcgtct tgtgcggagc
      241 gaaactggca cctatcttgc ccgatctttt gcgtctctcg cttcacgcga gaagccttgc
      301 tcagtcaaga aaggagccgg taatgcccgt gcagaaccct cgggacctgt ttatctacga
      361 cctcagcacc gtcctcgcgg cagagcaatc gatctcacag caactgtcac agatggagca
      421 ggcctgccag aacccgcagg tcaagcagat gttcagccag cacatcaccg acacccagcg
      481 acaggtgtca cgggtacagg actgcttccg gctgatcggc gcccaaccga tgaacgtgag
      541 ttgctacgcc gtagattgca tgcagcagga catgcaacag ttcgtccaac aggggcccag
      601 ccaggacatg atcgacatgt tctgcctggg ggcggctttg aagaccgagc actacgagat
      661 cgctgcctac agggggctcg tggagaaggc caggatgatg ggagagaccc agctagtctc
      721 cgtgctggag gacaacctga gggaagagat agacacctgc aagcaggtcc gccagatggg
      781 gacgcagatg ggctcccagg tgatccatag gatgcagcct taacagctat cagggcaaca
      841 gcggctgagg ggacggtcgg cggagaggaa gaaggtaggc ccggtagtca tctctcgggg
      901 gttggagcgt tcgtccacct ccatggcaat cacccccaat cgatccccca gccactctct
      961 gatcgccagc aaagcgatca gggctaccgg cctgtcggca ccctcggttt cagctgctat
     1021 cgtatctgga gcggtgttca cagtccggct tggatcgccc ttagtgtgag ggggaagtcc
     1081 atagctagtg aggtccttcc gctcggagcg gagaagaccc actccagttg gggatcgagc
     1141 gacacctctg agatcagccc taccctgtag caggttctgc tttccgcttc cgaagcgatt
     1201 tacgaaatcc ttacgcccgg gccaattctg gcaacagggc attaaccctg ttcccttgct
     1261 attgagatcg agtgcctgtt acgatcaacg gtaacaacac cgatcgatct ggaggtcggc
     1321 aagaatgtca tttgacgaaa atggcaaagg caaagaacgt gtaatcgcaa cgctggagcg
     1381 caactggaag gccgagatgg aaggcgcggc cacctatcgc gagctggcgt ccatggaaaa
     1441 tgacaagcgc cgccaatcgc tgctgatcaa gctggccgag gctgaagagc gccacgcgaa
     1501 gaagtggtcc gaccagatcg tgcagcttgg cggtgaggac ccatccaaga gggaaatcgc
     1561 catccccgca tctcgagggg taatcctcac cgcgaaggcg gcgggattgg agaccgcgtt
     1621 gcggcgagcc gagtccaccg aggaagaaca cgtccgtcag tacgagtctc aggctcgaga
     1681 actgggtgac gaggggatcg ccgccgtgct ccacgaactg gccgaggacg agtcgtccca
     1741 cgcaaccacc ctcagggcca tgacgggccc agcccagcag gtgcaaagcc ggctagaatc
     1801 gatcctgaag agggaaaagt ggcacggcaa gggcgggggg tggatcgggg acgctatata
     1861 cggtgtcaac gacgggctga ccgccgtctt cggcatcgtc tcgggcgtgg cggcggccag
     1921 cagcaacaac gagttcatcg tcgtggcagg actggccggc gttctctcaa gcgccctctc
     1981 catgggcgcc agcgcctacc tggccaacaa agcagagcgc gaggtatacg aggccgaact
     2041 cgcaagagaa agcagagaga tcgaggagaa ccccgaagag gaacgagagg aactggaact
     2101 attctaccag ctcaaggggt tcagcgagga agagtctcgc acgctggtgg agcggatggc
     2161 cgagaaacct gagcagttcc tcaaagccct ggcccatgag gagttgggcc tatccgagga
     2221 gcggctgcca aacccgaacc tggccgcagt tgtcggctcc gtctccacag ggttgggtgg
     2281 tatcgttccg gtaatcccct tcttcttcct ggccgggatg acggggatca tcgtctccct
     2341 ggtagtcagc atcattgccc acttcgcggt cggggcgtcc aagagcatag tgacgctgcg
     2401 aagttggtgg aagagcggct tggagatgac cggcgtggca ctgctggtgg cggtcgtcgc
     2461 ctacgcggtg ggcaacgcgc tggcgatcca ctgagtgctg ggtgctgaaa gatattgtac
     2521 cagcgggatc gagacgtagg gcacgtgacg cgagcgagcg cagcgtcgcg cttgtgcccc
     2581 gcctcccacg gcgccgccac gaccagcacg gtgattacga cctcgccgat ggcggggcac
     2641 aagaccccgc cctacgagac ggcgctgcgg gcgtcttgcc cgcagtagcg tgatgtagcc
     2701 acgcgtgggg gaccgattca atcaggatat acaggatatc ccggatgtac aggatgagaa
     2761 tggagaagac tcgtccttat cctgtatatc cgtctcatcc tgtacatcct gttcgtttcc
     2821 tctgtgcggg ccttcggccc gcctgcgggc gagacgcccg cgctcccagg attcatcacg
     2881 ccgtcgcact tcgcaacggt gtgccggtcc ccggccccta tcccccatcc cccaaccccc
     2941 atcccctcac cgttccgtgt agacttcgta cccctccttc cccaccacta cctcgacagt
     3001 cccaacccga tcggtccgaa ggatcgtcgc accacgcagc aggtctgcga cttcctctga
     3061 tgtgtgcgcg aaccgttctc cggctttccc ggagagaacc gccaccgtag gagacaccgt
     3121 ctgggcaaag cgctcctcca gcgcccctga agccccctgt cccggcactc ttagcagact
     3181 agccctctgc ggccaagcaa cggcgatctc ctcttggacg gaccgaggta tgtcaccggg
     3241 gaagagcaga ctggtcgagc cggagagcag cctcatcgac aaggtcggct gctctgcccc
     3301 attccccacc gaagagacag catcgacctc cagccacacg tcgtctccca acaaaatacg
     3361 ctgcccggcc agcggctcga aggtcggtat ctgccgctcc tccagcagtt cggcccaggc
     3421 tcttccagcg gcgctggacg gcggatggcc cggctgaacc accatgtcca cgcgccgccg
     3481 gtgggccacc tcgatgagac catccagccg gtttcggccg tagccggtaa gcaccaccgc
     3541 atccagcccg ctgtctccaa tcccctgata ccgccccagt gctcctgcaa gggccgatgg
     3601 actgccgccg ccatccacca gaacggtatg ccccgcaggg cccttgatca gcgtcgactc
     3661 cccgccaccc aactccagga aataaacggg cagcctgcca gcgggtcgac ccgaaatggc
     3721 gagaaccatt aggctgatga gggtaacggc cccgcccgcc aacaaccagc ggggcacgca
     3781 actcacggcc gcaagcaacc gctcacggaa gctcctcaac tccggcagcg gccagagtgc
     3841 aacacccagc agcgaaagcc aataggcggc gacgtaaaac gctgccggag cgcccagttg
     3901 gacggaagca gcaggcaacc gggcagtcca caggatcacc gccacgagat aggtaaggta
     3961 gagccatgca accggcgcaa gaaactcccc cagcggcttg gccacggcgc cggcggccac
     4021 gaccaccgtg ccgaccgcca tgatccccgg cagcattggc gcgatgagca ggttggtcaa
     4081 gggcgagacc agtgaaaggg tgtggaagct ggagaccaac accggcatcg ttgccagctg
     4141 tgcagcaagg gtcacggaca gagaggaggc cagccagccg ggcaacttgg acagacaggt
     4201 gcgcaggaca ggctccagca acaccagccc cgtggtggcg agaaaagata actggaagcc
     4261 gaggtcgtag agcaggggag gatccaatcc gaccatgacc gcaccggaga agaggaggaa
     4321 ggaaagggcg tcaccacccc gaccaaccag tatcgccagc agccccatgg tcgccatgag
     4381 ggcagcgcgt agcgcggatg gaggcagccc cgtgagcagg gaatagaccc agacggcgac
     4441 gatggccagc agcgtcgctt cgcgacgcgc cagcagtcga agcgccaaca gttgcgcaat
     4501 cccggcaact accgtgacgt tgaatccgga aacggcaacc acgtgactgg tgctggtaac
     4561 gttgagggca tcgcggaact ccaccggcat tctcgagcgt gagccaacga ggatcccgga
     4621 tagtaacgat gcgtgaggct ccggaagaag ctccacgcac aattcgtcca gccgggtccg
     4681 gacggcctca atcaaggtca tcggatcgtc agggctcgcc ctctccagaa gaccgatggt
     4741 cgggtactcg agagtcgtgt ggatgccctg tctggccagg taatccgccc agtagccgga
     4801 aaccggctgt acggcgcgaa gctccccccg aaccaccaac cggtccccat agccccactc
     4861 ctggtagcgg ctggtgcgca ccagcaggcg gccgcgagtc cccatccact cccccacccc
     4921 ctccggttgc agccggtcgg catccaggcg cagcagcaac gagttgtcct gggcctcggg
     4981 cctgttggaa acggtgccct ggatcgatac cctgccggtg cgttccagca gggtatccgc
     5041 ctcgggcacc acactgaagg cccgccagat ccccagcagt gctgtcgtca acgacaaggc
     5101 ggtgatcctg agcctgacat cgctaccggc gagcaatgcg gcgcccaggg cgcaggcggc
     5161 cagagccccc aaccatggac ccaacaacca cggatcgacc gcaaggacgt cgttcgcctg
     5221 tgtcccagtc acggccgcct ggccgaggca gagcccgaag agccagccga tacagaggaa
     5281 gatcagatcc acccgagagc cctccagcgg caagatcggg cgatggtcct acctgacctc
     5341 caccagcccc cgaatcttct cgaacgtgga ggcgttgacc agcttcgcct ccttcaattc
     5401 ttcaaccgaa ctgaagcggc cgttcttggc acgatagtcc agtatcctct gcgagagcac
     5461 cgtcccgatc ccgggcaagt cctccagctg cgcccgggta gcgctgttca gatccagctt
     5521 ggaagtgact ggagtggagg acgaagcgac ggcaatccca accggcaagg gcttttcccc
     5581 ggcaccagct gtctgaaccg gctgctcgga actggcttgg ctgtccgctc cggccgctgg
     5641 gatgacgacc tgttgtccgt ccttgagggg agttgccagg ttgaggaagg cggtttccgc
     5701 agtatcggta ggtccgccgg ccgccttcag ggcgtcctcg accctcgcat ctacaggcag
     5761 gctatacact ccggggccgg cgacggcccc agtcacgtgg accttgatgg atgccggcaa
     5821 gggggttgcg gcaggagata tcagttgcag tgcgggacgg cggggaagtt ggagaagggt
     5881 gaaggccgcg accgcgacca gcaccaccac tacagtagca aggttcgagc ggttccgctc
     5941 cagccattcc accatgggag caccccctct cgccgagaca cggtgacacg gtgacgaggc
     6001 ctggttactt cttgtcaggg gtgggtgtaa cgctcttggt cttggtgtac cactcagaaa
     6061 cgtgccggaa cctggaggtg gagtctatga aggcggagag cttgacatcc tttatggtcc
     6121 ggctcactgc ccagttgaat tgagggtaat agaggatcac ggccgggctt tcgtcctccc
     6181 agagttgctg gaactccgcg tactgttgag tccggatgct ccgatctatc gtgcggccgg
     6241 catcctccag cagcttgtct atcttctcgt tggccagacc ggagaagttg aagcccccgt
     6301 ttcccacctg gctggagtgc cacagggtat agacatcggg atcggcggtc atcagccact
     6361 gctcggctat gaccccctgg aaggtgcgct tcgccatgta ttctttcagc aaatcggacc
     6421 agccgagggc ctgcaactca catttgaacc ccaccttgcg cagatcctcg gcgatcatct
     6481 cacccgcctt gacccgccgg cggttgtcgt tggtgaggat cacgaaggtg agcggctggc
     6541 cgtccctgtc gcgaatccca tccccgttgc tgtccctcca ccccacggcg tccaagagcg
     6601 attcggcctt cttgggctca tactcgtagc tcttcgcctt ggtgtcaaac gcccaggagc
     6661 cgggggagat ggggcccttt cccggggatc cctggccatc catcacggtt cggatcagac
     6721 catcgcggtt gatggcatag gcaattgcct gccggacaga cttgtcttgg aagagggggg
     6781 aggtattgtt tatgaagagg accgtgtacc gaagatagtc gggcgatgag tatagcgcca
     6841 gcttctcgtt ggccttcagc ctggcaacat ccgaagaatc gatgttggca aaagcgtcca
     6901 catccccctt ctccaatgcc gcgagggcgc cggagtagtc tggatagaaa cggaaggaca
     6961 actgagccag gtggggcaca ggtccatagt attcggggtg aggctcgagg gtgataccat
     7021 cgccaccaat ccgggtcagc ttgaaaggtc ccgtgcccac gggatgcaag ttgtactggc
     7081 tgttggtcat cgccttgccg acggtatcgg aatacaggtg gcgcggtagt atgcccaccg
     7141 acgcaaactc caggaagggg ggcatagggc tccttcatgg taaagcgcac ggagtagtcg
     7201 tccacctgct ccacctggac gttgcgccac aggtcggcga catcctggct tccccggaaa
     7261 tcttccctct gcatctcccg tacagtgaaa accacatcgt cggcagtgaa gggggtgcgg
     7321 tcgtgccagc gcacgtctct ccgcaactga aagaggtagg tcttgccgcc atcggaaatg
     7381 tcccaccgct cggcgagatc ggggacaatg ttcccgttct cgtcaaaacg ggtaagaccg
     7441 ctgaagatga gagacgagac atctctctcc acctggttga attggagaag gatggggttc
     7501 agataggagg gattcccgat caccgcctcg gtgtagctcc cgccggagtc ggggaccgaa
     7561 accgtgggga tctccctggg aaacttgctg gctgccacgg ggatcaacac ggccaagagt
     7621 gccacgacaa tcagcacccg gcgcacgcta atacccctct tggcgactct gtacgccctc
     7681 gagtccatgc agatcgaact ccaccagggc aagagtgtgg ggactggaga aagctccggc
     7741 cccatttggg gccggcccaa ctgtaagcac gcagaagcag cctggaaccc gacggcctag
     7801 gagaagaact tcaccgagac gatgctgagc agcacgaagg ccaccgccag gccgatagtg
     7861 aagttgtaca gggtgcgctc caccccgcgc cggctgcgat agacagagga gtcgcccccg
     7921 cccaaggccc cagcgaagcc agaccccttt gcctggagaa gcactaccag gatcaacact
     7981 atcgaaatga gtatctgcac caggttcaga taggtctgca aaagacgcac ctccagtaat
     8041 tcaggcaacg atgattataa cacgggccaa actcaggcag caaacgccct tggtgctcca
     8101 agaagaaagt gctacactcg ccgccatcag tcaggcatct ggtgtccttc gacagcaaag
     8161 gcaaccagtg gagagggatt gaaaggaggt ctttttggca gaagtagaga taggaatact
     8221 gactcgcggc aaggctaccc ttggcatgac gctttgcagc ctgctgcttc agcaggttca
     8281 cgatatacgc atcttcatag tggatacctc tgagaagccg gtcattcaga gggatgacgt
     8341 ggcctttgcc ctgaggctgg ccttcgaccg gcagattcag tgcaactacg agtacctcag
     8401 ggacagggaa agggccttca gcgtcggaag attgaagatc ctggaggcgt tgaagggaag
     8461 gaatctctgt ttcatggatg acgacatagt cctgccgtcg tcttcacttg ccaggctgct
     8521 ggaggacacc attccggagt cgaaatacgg cttcgtcgcg ccctactgcc ggcactcagc
     8581 ctttccaccg ggcttcgctt ccgacacccc cctctacgcc ccaggaaccc tcttctgcca
     8641 ggacgctatg gtgagggaga tcctgctcga ctactatcga accaccgtgg acgtactgga
     8701 cagggctccg tcgcccaaca aggtgtg
//
LOCUS       DATALS010000250         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_249937, whole genome shotgun sequence.
ACCESSION   DATALS010000250 DATALS010000000
VERSION     DATALS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249937"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..581
                     /locus_tag="VHS06_05605"
     CDS             186..581
                     /locus_tag="VHS06_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX2987629.1"
                     /translation="MGDWHTEIDVRGVHKFEGDESHKDGGMDAGPSPGELLLASVATC
                     MCLAVKHIAQKRRVAIRGLSVDATGEADREAFRYGEVHLVVHAELPQEELNAIVAVAK
                     RYCWVSNTLAGGCPVVTTAEPVVMVEADK"
     gene            578..2119
                     /locus_tag="VHS06_05610"
     CDS             578..2119
                     /locus_tag="VHS06_05610"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEX2987630.1"
                     /translation="MKTDNGGSIDTALFRTPLQVVNLGIQDFARELRQQSVPVAEVDW
                     RPPAGGNVEMAELLKQLYGAPRGFVPAVGAPRPLVDLIDSANREVIDRIVRSQPRVVD
                     VAPAREAMGLPPYTVLHSGPPISWERMCGPQKRGILGAILFEGWASDPRSAEEMVKSG
                     RVTFGPSHEYDAVGPMTGIVSPSMPVLVVRNETFGNHGFATFNEGRGNVLWFGVCNEG
                     SLDRLRWMRDTLGPAVKATILKNGPIDVFDVDAQGLQMGDECHARSAACTATLIKTMA
                     MGMLDADLDSRTIASVIRFIADNSHFFLNFTMAAVKATMDAAHGVPYSTIVTAISRNG
                     EDCMVRVSGLGDRWTVVPAARMQEALFYTGFSVADAAGDIGDSAIIETCGLGGMAIPA
                     APSMAVFVGGSLNDEVAAVRELRDITVSRHPKFTLPTMGFEQTPLGIDVRRVVETRTT
                     PFITTGVLHESSETTGQIGTGVAHIPVEAFEQALIALAMEWGLATPLPEPTSIVRIQS
                     RRGVQ"
     gene            2116..>2776
                     /locus_tag="VHS06_05615"
     CDS             2116..>2776
                     /locus_tag="VHS06_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987631.1"
                     /translation="MTFEADLRECVIAWRRAEDLYYRSVLQEPSLYTSSINLVRAIAD
                     SLAEITELAALVGEYRVADPELVATIADSLELPHREFIDYDLALDAGYYLRYQEIAEI
                     ESAAEIRDRIEKARSEGDEWVVLYDNKTRSRGHTYFERLEMQLSNGIGLRTSSELDWE
                     KGRIFTVEPINLDPETGQMRRGVRSSDPMQEFTTLGEMAAAVEALRVKYSGTGRDATD
                     AG"
BASE COUNT          524 a          786 c          954 g          512 t
ORIGIN      
        1 cggcaccggg acgaagccct atcccaagtc gggttggatc tatccactga tgcgcgttgg
       61 gttcctgccc gcctaccgcc tggtgccctg gccaatgcgc aaatggttcc tgaggctgtt
      121 ttttgtccgc ggcccgcagc actgggcgaa ctggtaggca tatggagcca ataagagttc
      181 gctggttggg cgattggcac acggagatcg acgttcgggg cgttcacaag ttcgagggag
      241 acgagagcca caaggacggc ggaatggatg cggggccatc gcccggcgaa ctactcctgg
      301 cgtctgtagc gacctgtatg tgtctggcgg tgaagcatat tgctcagaag cggcgtgtgg
      361 ctattcgcgg tctctcggtg gatgctacgg gagaggccga tcgcgaggca tttcggtacg
      421 gcgaggtcca cctggtggta cacgccgagt tgccgcagga ggagctcaat gccatcgtcg
      481 ccgtggccaa acgctactgt tgggtgagca acaccctggc tggtggctgc ccggtggtga
      541 cgacagccga acctgttgtc atggtggagg cagataagtg aagacagaca atgggggctc
      601 gatcgacact gcgctgtttc gcacgcccct ccaggtggtg aacctgggta tccaggactt
      661 tgcccgcgag ctgcgccagc agtcggtccc tgtggcagag gtggactggc gtccaccggc
      721 agggggaaac gtcgagatgg cggaactgct gaagcagctg tacggcgccc ccaggggctt
      781 tgtacctgct gtcggcgcgc cgcgaccgct ggtggacctg atcgactccg ctaaccgtga
      841 ggttatcgac aggatagttc gatcccagcc cagggtggtg gacgttgcgc ctgctcggga
      901 ggcgatgggc ctcccgccct acacagtact gcattccggg ccaccaataa gctgggagcg
      961 gatgtgcgga ccgcagaaac gggggattct gggcgccatc ctgttcgagg ggtgggcgag
     1021 cgatccccgc tccgccgagg agatggtgaa gagcgggcgc gtaaccttcg gtccatccca
     1081 cgagtacgac gccgtgggac ctatgaccgg gatagtctcg ccatccatgc ccgtcctggt
     1141 cgtgcgcaac gagaccttcg gcaaccacgg cttcgccacc ttcaacgagg gacgtggcaa
     1201 cgtcctctgg tttggggtgt gcaacgaagg ttccctggat cggttgcgct ggatgagaga
     1261 tacgctgggg cccgcggtga aggcgacaat cctgaaaaac gggccgatag acgtgttcga
     1321 cgtggatgcg cagggactgc aaatgggtga cgagtgccac gcccgaagcg ccgcctgcac
     1381 ggctacgctg atcaagacga tggcgatggg aatgctggac gccgacctcg atagtcgcac
     1441 catcgcctcg gtgatccgct tcattgccga caacagccac ttcttcttga acttcaccat
     1501 ggccgcggtg aaggcgacga tggatgccgc ccatggcgtc ccttacagca ccatcgtcac
     1561 ggcaatatcc cgcaacggcg aggattgcat ggtacgggtg agcgggctgg gcgaccgctg
     1621 gaccgtggtg ccggccgcga ggatgcagga ggcgctcttc tacaccggct tttccgtcgc
     1681 ggacgcggcg ggggatatcg gggacagcgc catcatagag acctgcgggc tggggggaat
     1741 ggcaatcccg gcggccccaa gcatggcggt cttcgtcggt ggcagcctga acgacgaggt
     1801 ggccgcggtg cgggagctac gcgacataac ggtgtcgcgc catcccaagt ttaccctgcc
     1861 gaccatgggc ttcgagcaga cgccgctggg gatcgacgtt cgccgcgtcg tcgagactcg
     1921 cactacgccc ttcatcacga ccggcgtgct gcacgaaagc agcgagacta cggggcagat
     1981 tgggactggg gttgcccaca tcccggtgga agcatttgag caagccttga tagcgctggc
     2041 catggaatgg ggattggcaa ctccgttgcc cgaacctacc tccatcgtga ggatccagtc
     2101 caggagaggc gtgcagtgac attcgaggcc gaccttcgcg agtgtgtgat tgcctggcgc
     2161 cgagcggagg atctctacta ccggtcggtg ttgcaggagc cttctctata caccagcagc
     2221 atcaatctgg tgcgggcgat tgccgacagc ctggcggaga tcaccgagtt ggctgcgctg
     2281 gtcggtgagt accgggtggc cgatcccgag ttggtggcaa ccatcgccga ttcgttggaa
     2341 ctgcctcacc gagagttcat cgactacgac cttgcgctcg atgccgggta ttaccttcga
     2401 taccaggaga tcgcggagat cgagagcgct gccgagatac gggatcggat agagaaggct
     2461 cgatccgaag gtgacgagtg ggtggtgctg tacgacaaca agacgcgcag tcgtggccac
     2521 acctacttcg agcgattgga gatgcagctg tccaacggga tcgggttgcg taccagctcc
     2581 gagttggatt gggagaaggg ccgcatcttt accgttgagc cgatcaacct ggacccggaa
     2641 acggggcaga tgagacgggg tgtacgttcc tccgacccga tgcaggagtt cacgacgctg
     2701 ggagagatgg cggcggccgt tgaggcgcta cgggtgaagt actccggcac aggtcgggac
     2761 gctactgatg ctggac
//
LOCUS       DATALS010000251         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_207998, whole genome shotgun sequence.
ACCESSION   DATALS010000251 DATALS010000000
VERSION     DATALS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207998"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VHS06_05620"
     CDS             <1..311
                     /locus_tag="VHS06_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX2987632.1"
                     /translation="ADRRFVHARAIREDEAVDLELEESATAAIRLYESYLLEAGASDL
                     DAPPPHKAIMTAFWESHNAQVLRRVGLAEDVVYCSQLDISNVVPQLQIEGDRMVLSRE
                     "
     gene            353..1135
                     /gene="nth"
                     /locus_tag="VHS06_05625"
     CDS             353..1135
                     /gene="nth"
                     /locus_tag="VHS06_05625"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX2987633.1"
                     /translation="MPATVMEERKGDWLVSEELARVEVAGLRERIDEIGRLLESEYGR
                     PAPRRKLRPLDELILTILSQHTTDANSGRAFERLKSAFPSWEEARDAEPDEIAEAIRC
                     GGLARIKAVRIKQLLQHLDAELGRLDLDFLCDMEMEEARRYLLGLEGVGPKTAACVLL
                     FSCNLPAFPVDTHVHRVSRRLGLIPPTASAEDAHQILQKAVPVGEVYSFHVNLITHGR
                     RVCKAQRPLCDQCGLALHCDYFTGRLGTEPPEGDGTASDRGM"
     gene            complement(1417..>1732)
                     /locus_tag="VHS06_05630"
     CDS             complement(1417..>1732)
                     /locus_tag="VHS06_05630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX2987634.1"
                     /translation="GEGGHSGLDIATAMGSPVRAAAGGTVVNLQQTQIDYGWYVVIDH
                     GNGLKTLYAHLSAFNVKLGDTVSRGQQVGAVGTTGRATGPHLHFEVRSSGRLVNPLNY
                     FP"
BASE COUNT          309 a          508 c          601 g          314 t
ORIGIN      
        1 aggccgatag acgcttcgtg cacgcgcggg ccattcggga ggatgaggcg gtcgacctgg
       61 agcttgagga gtcggccacg gcggccatcc gtctttacga gagctacctg ctggaggccg
      121 gggcctcgga cctggacgcc ccaccgcctc acaaggcgat aatgacggcc ttctgggagt
      181 cgcacaacgc ccaggtcctg cgcagggtgg gactggccga ggatgtggtg tactgctcgc
      241 aactcgacat aagcaatgta gtgccgcagc ttcagatcga gggcgatcgc atggtgctgt
      301 cccgagaata gcaggtatgg gcggtcggag acgccccggt tgcggtggtg gaatgccggc
      361 gacggtcatg gaagaacgga agggggactg gctggtgtcg gaggaactgg ctcgggttga
      421 agtagcgggg ctgcgggaga ggatagacga aatagggaga ctgttggagt cggagtatgg
      481 acggcctgcc ccccggcgaa agttgcgacc gctggacgag ctgattctga ccattctctc
      541 tcagcacacc accgacgcaa actccggccg ggccttcgag aggctaaagt cggccttccc
      601 ctcctgggag gaagcccgcg acgccgagcc ggatgagatc gccgaagcga tccgatgtgg
      661 ggggcttgcc cgtatcaagg cggtgcgcat caaacagctg ctgcagcacc tcgatgccga
      721 gttggggaga ttggatctgg acttcctgtg cgacatggag atggaggagg ccaggcgata
      781 cctgcttgga ttggaagggg tagggccgaa gacggcggcc tgtgtgctgc tcttttcctg
      841 taatcttccg gcgttccccg tggacaccca cgtccatcgt gtgtccaggc gactggggct
      901 tatccctccc acggcctctg ccgaggacgc gcatcagatc ctgcagaagg cggttccggt
      961 aggggaggtg tattccttcc acgtgaacct gatcacccac ggccgcaggg tctgcaaggc
     1021 gcagcgtccg ctgtgtgacc agtgcggtct ggcacttcat tgcgactact tcaccggcag
     1081 gctcggaaca gagccgccgg agggggacgg caccgcatcc gaccgaggga tgtgacggta
     1141 gcgagcctgg catttcggac acgcatgcgc cccgccaaat gcatgggtat agtagccgag
     1201 gagggtggtg cgatccttgg aaggagcgta gggcggggtc ttgtgccccg cctacagcat
     1261 ggccgaaatc atcgtggggg tcttatccgc ggtgtgggcg gcggggcaca agaccccgcc
     1321 ctacgagacg tcatgagtgg cccctcggct cctcgcagtt gccagcgagg cgtcaaccgg
     1381 gcagtggcgg ttgacaggga tggttgccct cagggactac gggaagtaat tcagcgggtt
     1441 gaccagcctg ccgctggatc gcacctcgaa gtgcaggtgc gggccagttg cccttcctgt
     1501 ggttccgact gcgccgacct gctgcccccg actcaccgtg tcccccagct tgacgttgaa
     1561 ggcactcaag tgggcataca gtgtcttcag accgttgcca tggtcgatca cgacgtacca
     1621 gccgtagtcg atctgcgtct gctgcaggtt caccaccgta ccgcccgctg cagcgcgcac
     1681 ggggctcccc atggctgttg cgatgtccaa tccggagtgg ccgccctcgc cg
//
LOCUS       DATALS010000252         4036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_418451, whole genome shotgun sequence.
ACCESSION   DATALS010000252 DATALS010000000
VERSION     DATALS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418451"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VHS06_05635"
     CDS             complement(<1..858)
                     /locus_tag="VHS06_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX2987635.1"
                     /translation="MKILIVYTNSYRMLAAAPLGASLVASRLRKDGHEVRLLDLMFDK
                     TPARSAAWAARAYQPDLIGFSIRNVDNQSPSQFYDPLPAIKEIVSSVRAAWPVPTLLG
                     GTALTTFPGQFLTELEADFGIAGDDLDPISRFVASLESGSPDLSVPGLVYRDGEGIHC
                     NPYQIRGYADTVFDGWDLMRLRPYRRSFDAMWDAGIVVRSGCPFQCVFCDTYRSYGRE
                     WVLRDPKQVAEELLALKRIHGVRSVFLADAGFNRPLDHAKAVLEEIIRANVGVGISAV
                     FEHGEGDREF"
     gene            complement(855..1670)
                     /locus_tag="VHS06_05640"
     CDS             complement(855..1670)
                     /locus_tag="VHS06_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2987636.1"
                     /translation="MTQNREPMGNCTGNCAYCETRGTPRRDGTMPMCVRVESETVLRM
                     SGTAGLETIGPERGLDPYDQGEGRCPETRQRLEKLLQDGRPARVITRSARIVEDLPLL
                     SEIARRASATVLIGVATVDAELARVWEPQLLAPDRRLALMRRIREAGVSAGLMAAPLV
                     PFLMDDHPQLRSLLWAAQQSGAQYYCVDPTAWGEELALDRLMPIFAERWPQLLEPLQR
                     LYGGEKSPPRAYTDKIAGQIERLAPGARLSDRNPAAWSPGALLANAFASHYQR"
     gene            complement(1667..2629)
                     /locus_tag="VHS06_05645"
     CDS             complement(1667..2629)
                     /locus_tag="VHS06_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX2987637.1"
                     /translation="MTAPPIAFWRHWPIDDQEAESLARATLEFQRRFDWDFIKVTPSH
                     TFCLDDYGAKHAYRGKPIGDRDHLERVVQQPEDWDKIEPVDVHKGAYSRQLRSLRMVI
                     DGREGDTPVLQTLFNPLSMARYLAGDEAYLVHLRREPQRVKRALDALTKTCSDFAAAA
                     IAEGADGIFLSTSSASYEAMSEEEYRGFGMPGDLAILQAAGKGWFNLLHLHGQNPMFN
                     LAASYPIQAINWHDRTAWPSLSEAATIFPGALVGGVEQYQLLHFGTPTEVESQVHDAI
                     AQMGGRRLIVAAGCTFPLTVPEGNLRAARRAVDSYQAKQVGTQA"
     gene            3094..3435
                     /locus_tag="VHS06_05650"
     CDS             3094..3435
                     /locus_tag="VHS06_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX2987638.1"
                     /translation="MPYSERTLTCRDCGRSFQFTVEEQEFFAQKGFTNDPVRCPECRA
                     ARRAERNAGSRGGYGSGSGYGRESRQMYPAVCARCGKDTEVPFQPRGDKPVYCSDCFA
                     QERGSSFGWGR"
     gene            3423..3674
                     /gene="rpsU"
                     /locus_tag="VHS06_05655"
     CDS             3423..3674
                     /gene="rpsU"
                     /locus_tag="VHS06_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019502896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX2987639.1"
                     /translation="MGTVNGDMQVIARDGEPIEELLSRFKRGMVRSGILRDLKKHRFF
                     VGPGEQRRLKAKEAARKMKRRQQKAADRRKRGGFGSRSR"
     gene            3759..>4036
                     /locus_tag="VHS06_05660"
     CDS             3759..>4036
                     /locus_tag="VHS06_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987640.1"
                     /translation="MLEISDEACELLLDKLSEALEGRPELRRRPTPPGLKLCIHKNAA
                     HLSLSFPSEGDKVVSYQGKSVLIIAAKDLDELSGACFLVRHCGNEDRLA"
BASE COUNT          685 a         1231 c         1360 g          760 t
ORIGIN      
        1 gaactcccgg tccccttcgc cgtgctcgaa gacggcgctg atgcccacgc ccacgttggc
       61 ccggatgatc tcttccagaa cggccttggc gtggtccagg ggacggttga agccggcgtc
      121 ggcgaggaag acggagcgca cgccgtggat ccgtttcagc gccagcagct cttcggctac
      181 ctgcttcgga tcgcgcagca cccattccct gccgtagctg cgataggtgt cgcagaacac
      241 acactggaat gggcagccgg atcgcacgac gatcccggca tcccacatgg catcgaagga
      301 gcggcggtag gggcgcagtc tcatcaggtc ccagccgtcg aagacggtgt cggcgtatcc
      361 gcggatctgg taggggttgc agtggatccc ttctccgtcg cggtagacga ggcccggtac
      421 ggagaggtcg gggctgccgg attccagcga cgccacgaat cgggagatgg ggtccaggtc
      481 atctccggcg atgccgaagt cggcctccag ctcggtcaga aactggccgg ggaaggtggt
      541 taaggccgtt ccgcccagca gcgtggggac tggccaggcc gcgcgcaccg aggaaacgat
      601 ctccttgatg gcggggagcg gatcgtagaa ctggctgggg gactggttgt ccacgttgcg
      661 gatggagaag ccgatcaggt cgggttggta ggccctggcg gcccatgcgg cgctccgagc
      721 cggcgtcttg tcgaacatca ggtccagcag ccgcacctcg tgcccatcct ttcggagacg
      781 cgaggcgacg agggatgctc ccagcggggc ggccgccagc atccgataac tattggtgta
      841 gacgatcagt atcttcatcg ttggtagtga ctcgcgaagg cgttggccag cagggcgcct
      901 gggctccaag cggccgggtt ccggtcggaa agccgcgcgc ccggcgccag tcgctcgatt
      961 tggccggcga tcttatccgt gtatgccctg gggggggact tctccccacc gtacagccgc
     1021 tgcagcggct caagcagctg tggccaacgt tcggcgaaga tcggcatcag ccggtcgagg
     1081 gccagttctt ccccccatgc cgtggggtcc acgcagtagt actgtgcgcc cgactgttgt
     1141 gccgcccaca gcaggctccg cagctgaggg tggtcgtcca tcaggaatgg caccagcggc
     1201 gccgccatca ggccggcact caccccagcc tcccgtatcc ggcgcatcag cgccagccgc
     1261 ctgtcgggag ccaacagctg tggctcccag acccgggcaa gttcggcatc cacggtcgca
     1321 acgccgatca gcaccgtggc agaggcgcgc cgcgcgatct ccgacagcag cggaaggtcc
     1381 tccacgattc gggcggagcg ggtgatcacc cgcgcagggc ggccgtcctg cagcagtttt
     1441 tcgagcctct gtcgcgtctc cgggcaacgc ccctccccct gatcgtaggg atcgaggcct
     1501 ctttctgggc ctatggtctc caaccccgcg gtgcccgaca tccggagtac cgtctctgac
     1561 tcgacacgaa cgcacatcgg catcgtgccg tcccgtcgcg gggtccccct ggtctcacag
     1621 taggcgcagt tcccggtgca gttgcccatc ggttccctgt tctgggtcat gcttgtgtcc
     1681 ccacctgttt tgcctggtag ctatccacgg cgcggcgtgc cgctcggagg ttgccctcgg
     1741 gcacggtcag cgggaaggtg cagccggcag ccactatcag cctgcggccg cccatctggg
     1801 cgatggcgtc gtgaacctga gactccacct ccgtcggggt cccgaagtgg agcaactggt
     1861 actgctccac gccgcctacc agcgccccgg ggaagatggt cgcggcctcc gaaaggctcg
     1921 gccacgccgt gcggtcgtgc cagttgatgg cctggatggg gtaggatgcg gcgaggttga
     1981 acatcgggtt ctggccgtgc aggtgcagca ggttgaacca gccctttccg gccgcctgca
     2041 ggatggcgag gtcgccgggc ataccgaagc cgcgatactc ctcctcgctc atagcctcgt
     2101 agctggccga tgaggtggag aggaagatgc cgtccgcgcc ctccgcgata gcggccgcgg
     2161 cgaagtcgga gcaggtcttg gtgagagcgt ccagtgcccg cttcaccctc tgcggctccc
     2221 gccgaagatg gacgaggtat gcttcgtcgc ccgccaggta tcgggccatg ctgagcgggt
     2281 tgaagagggt ctggagcacc ggggtgtccc cttcccgccc gtctatcacc attcgaaggc
     2341 tgcgcagctg cctgctgtat gcccccttgt ggacgtctac cggctcgatc ttgtcccagt
     2401 cctcgggttg ttggaccacc cgctccaggt ggtcccggtc gcctatcggc ttgccacggt
     2461 aggcgtgctt cgccccgtag tcgtcgaggc agaaggtgtg ggagggggtg accttgatga
     2521 agtcccagtc gaagcgacgc tggaactcca gggtggcccg tgccagcgac tcagcttcct
     2581 ggtcgtcgat gggccagtgc cgccagaagg cgatgggggg ggcggtcaac tgcttcccct
     2641 gccagggttg cccggattcg ttccctcttg tccatgtcgg ttcctcccaa gcgatgggcg
     2701 gggctgccct tccagggacg ccctgcaagg aagacggcga ggaatggagt tgtagaagtt
     2761 gtccgtgggc caccctatca tccggccggt ttgccgtcaa tcatcgcgtg gtctgctgcc
     2821 ccgggttggc gagccgattg acggatgggt tgagtccgcg catatgcccg aggcatgaat
     2881 agtgagaagg tatgacaaag gaatcgccta gatgaataga atgtggtaga ctactacaga
     2941 tggtggcatc gttggaccat atttgtgggt acccgcttgt tcctctcgcg gaggccctca
     3001 cgaagagtcg acgattcccc ctgatccgga tcgaccatgg ggtcgtattg tgctgtctgc
     3061 cagcggatag cgattagtta ctaaggagaa gaaatgccct attcggagag gaccctcacc
     3121 tgccgcgact gcgggcgctc tttccagttc acggtagaag agcaggagtt cttcgcgcaa
     3181 aaaggcttca ccaacgaccc ggttcgttgt ccggagtgcc gtgcagcgcg cagagcagag
     3241 cggaatgctg gttcgcgcgg cggttatggc agtggtagcg gctacggtcg tgagtcccgg
     3301 cagatgtatc cggcagtatg tgctcgctgc ggcaaggaca ctgaagttcc gttccagccc
     3361 cgcggcgaca agccagtgta ctgctccgac tgcttcgcgc aggaacgggg ttctagcttt
     3421 ggatggggac ggtaaacgga gatatgcagg taattgcacg agacggcgaa ccgattgagg
     3481 agcttctctc tcgcttcaag cgaggaatgg tcaggtctgg gatcctccgc gatctcaaga
     3541 agcatcgctt cttcgtgggc cctggcgagc agcgccggct caaggctaag gaagcggcca
     3601 ggaagatgaa gaggcgccag cagaaggcgg ctgacaggcg aaagcgtggg ggcttcgggt
     3661 cccgctccag gtagacagcc ggtgaggcta cactccggcg actgaatcgg tcggctttag
     3721 tttcatcgtg ctcatgggcg taagggcgga ggtatccaat gttagagatc tctgacgagg
     3781 cctgcgagct gttgttagac aagctctccg aggcgctgga ggggcgaccg gaactgaggc
     3841 gccgcccgac tccgcccggc ctcaagctgt gcattcataa gaatgccgcc cacctgtccc
     3901 tcagctttcc cagcgagggg gacaaggttg tgagttatca gggcaagtcg gtgttgatca
     3961 tcgctgccaa ggatctggac gagctctcag gggcttgctt cttggtccgc cattgcggca
     4021 acgaggatcg gctggc
//
LOCUS       DATALS010000253         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_282202, whole genome shotgun sequence.
ACCESSION   DATALS010000253 DATALS010000000
VERSION     DATALS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282202"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VHS06_05665"
     CDS             complement(<1..711)
                     /locus_tag="VHS06_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2987641.1"
                     /translation="MTHWVIWARRGIQGAALLLFLYLAVAARFPLASALPVDLFFRLD
                     PLAQITASLASRQLAPYALWALPLLVSTALIGRFFCGWLCPLGASLDLLRLRVASRPQ
                     FKREKELRGLKYLLLAAVLVAGLAGSTAPMLLDPITLLVRSFVVSLYPALNAAVTGVL
                     FTLYDHGILPELMAWIDTDLRTNLLPAAQPVYQLGWLFLTIFAAVIATNLVAHRFWCR
                     YLCPLGAMLSLLSRISFFG"
     gene            complement(708..1757)
                     /locus_tag="VHS06_05670"
     CDS             complement(708..1757)
                     /locus_tag="VHS06_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX2987642.1"
                     /translation="MSRPINRRRFLRNLLLLGAGYGILPNVACSPSGQAVPTPAVRLV
                     PTAAPTSTALSSPAGVPNAAAVATIGPTVASTVATSSSQLVVARGSSPEAITRSAIET
                     LGGMSRFVKPGAEVIVKPNICVANYSFEYAATTNPEVVGTIVKLCVEAGAKRVRVMDF
                     PFGGTAEQAYVKSGIGDAARAAGGQMETMSSLKYGDVAIPNGRDIKTWKIYRDAVDPN
                     VVLINVPIVKHHSLAGVTIGMKNLMGLIQDRGGIHTNMDQRLPDLLTVIRPTLTVVDA
                     VRILTRNGPTGGNLADVKKLDTVVASADPVAADSYGATLFGRSGSDLGYIRNGAAMGL
                     GVMDLSQVAIKEVTA"
     gene            complement(1929..>2243)
                     /locus_tag="VHS06_05675"
     CDS             complement(1929..>2243)
                     /locus_tag="VHS06_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEX2987643.1"
                     /translation="LDGKGQRRTIPPLTLTMNDLFRREKFAHLPRAISEDGRRTHTYE
                     VGDIAYWSPGPDVATFYRHDGQEIPNPGIILIGKIDSGVEALDVAGSVKVKTEEDRTS
                     GR"
BASE COUNT          435 a          676 c          720 g          412 t
ORIGIN      
        1 accgaagaag gagattcgcg ataggagaga gagcatcgcc cccagcgggc acaggtagcg
       61 gcaccagaat cgatgggcca ccaggttggt agcgatcacg gcggcgaaga ttgtcaggaa
      121 cagccagccg agctgataca caggctgggc ggccggcaga agatttgtgc gcaggtctgt
      181 gtcgatccac gccatcagtt ccggcaggat gccgtggtcg tagagtgtga acagcacgcc
      241 tgtgacggcg gcattaaggg ccgggtagag cgatacaacg aagctgcgaa ccaacaaggt
      301 gatggggtcc agcagcatcg gcgccgtcga tccggcaagc ccggccacca ggacggccgc
      361 gaggagtaga tacttgaggc cccgcaactc cttttcccgc ttgaactgtg gccgggacgc
      421 cacccgaagc cgcaacaggt ccaggctagc gcccaagggg cagagccacc cacagaagaa
      481 gcggccgatg agcgcggtgg acacgaggag cggcagagcc cacagggcgt acggcgcgag
      541 ttgtcgcgag gccagggatg cggtgatttg ggcgagcgga tcgagcctga agaagagatc
      601 caccggcaac gccgaagcca atgggaagcg tgctgccacg gcgaggtaga gaaagagaag
      661 cagcgccgcg ccctggatac ctcggcgcgc ccagatgacc cagtgagtca tgcggtcacc
      721 tctttgatgg ccacctgcga gaggtccatc actcccagcc ccatggctgc accgttacgg
      781 atgtagccga ggtcggagcc gctcctgcca aacagggtcg caccgtagga atcggccgcg
      841 acagggtcgg cactcgcgac cacggtgtcg agcttcttga cgtccgcgag gttgccgccg
      901 gtgggaccgt tgcgggtcag gatgcgcacg gcgtccacca cggtgagggt aggacgtatc
      961 actgtgagta ggtcgggaag gcgctggtcc atgttggtgt gaatccctcc cctgtcctgg
     1021 ataagcccca tcaggttctt catgccaatg gtgacaccgg ccaggctgtg gtgcttcact
     1081 atcggcacgt tgatcaggac gacgttcggg tccaccgcat cgcggtagat cttccacgtc
     1141 ttgatgtcgc ggccgttggg gatggctacg tcgccgtact tcaggctgga catcgtctcc
     1201 atctgaccgc ccgcggccct ggctgcatca ccgatgccgc tcttcacgta ggcctgctcg
     1261 gccgtcccgc cgaagggaaa gtccatcact cgcacccttt tcgccccggc ctccacgcag
     1321 agcttcacga tggtgcccac gacctcgggg ttagtcgtgg cggcgtattc gaaggagtag
     1381 ttggcgacgc agatgttcgg cttgactatg acctccgccc cgggcttcac gaaccgactc
     1441 ataccaccga gggtctctat cgcggatcga gtgatcgcct ccgggctgga accacgcgcc
     1501 accacaagct gggagctact ggtcgcgacg gttgacgcaa cggtaggccc gattgtggcg
     1561 accgccgcgg cattagggac accggcgggc gacgaaagcg ccgtggatgt tggggccgcc
     1621 gtgggcacca agcgcacggc gggtgtaggg acggcctgtc ccgatgggga gcaggcgacg
     1681 ttcggcaaga tgccgtagcc cgctcccaga agcagaaggt tccgcaggaa cctgcgacga
     1741 ttaataggtc ggctcatctt cgagtgtccc ttcttactga tacgaggatg ggctcgtgaa
     1801 ggtgaccttg gatctacaat ggtgcacgag cactactttc gccgatcagc gaccggtcat
     1861 tcccatgcag ccgagcctga gcgtcgaggc ctcaaggccg cttttcccca gtctacgctt
     1921 ctccatcgtt atctcccaga tgtcctgtct tcctcagtct tcaccttcac cgagccagct
     1981 acatcgaggg cctctacgcc agagtctatc ttccctatga ggatgatgcc tgggttgggg
     2041 atttcctggc cgtcgtggcg atagaaggtg gcgacgtcag ggcctggaga ccagtaggcg
     2101 atgtccccga cttcgtacgt gtgcgtgcgt cttccatctt ccgaaatcgc tctcggaaga
     2161 tgggcgaact tctctctacg aaacagatca ttcattgtca gcgttagcgg cggtattgtt
     2221 cgtcgctgac cttttccatc cag
//
LOCUS       DATALS010000254         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_418546, whole genome shotgun sequence.
ACCESSION   DATALS010000254 DATALS010000000
VERSION     DATALS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418546"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..543)
                     /locus_tag="VHS06_05680"
     CDS             complement(184..543)
                     /locus_tag="VHS06_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987644.1"
                     /translation="MGHNRVEDALNRLGERGLLWGLMVVALASHAFNMFGYPLYLGDE
                     GIYMEQAWSVIRLGKLAPYTYFYDHAPAGWLQIALWLAILPKGAYTFGMAVASGRVLM
                     LILHLTSVPELFCDRSI"
     gene            complement(544..>1773)
                     /locus_tag="VHS06_05685"
     CDS             complement(544..>1773)
                     /locus_tag="VHS06_05685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2987645.1"
                     /translation="IESRTQNIMMYTWDRPEAYQRARAPDTFRVPEKSMVVLLPARHE
                     EEVIQETIGRVVHANYPSRLMEVLVICEHTDAGTIEKVDEEIAALKREHINNVRLVVF
                     NDTPVNKPHGLNIGLRDSTNEVVTVFDAEDEIHPEIFNIVNTVMLEEGVRVVQCGVQL
                     MNYDSSWYSALNVLEYFFWFKSRLHYHAKLGATPLGGNTVFFERPLLDEIGGWDEHNL
                     TEDADVGMRISAMSEPVRIVYDDQYVTREETPPTVGHFIRQRTRWSQGFLQTLRKGDW
                     TRLPNWKQRVMAAYTLGFPTAQAVLALYVPISLWMMFSTKVPVGQALLLLLPAYLLVS
                     HYFMSLIGLYEFADAHGLHATWKTPVAMALAYLPYQWMLAYAAFRAFIREVRGVSNWE
                     KTQHVGAHRADQGQRAA"
BASE COUNT          359 a          561 c          516 g          337 t
ORIGIN      
        1 cttcgaactg acggggaaga acacgctaat cttcatcggg gagaagagcc aggcgaccgc
       61 tacggcgggc ttcgacgttc tgccctatac accgaacgtg cagccatcca cttacggtgg
      121 ccggcctggt actgccatta cgttctacgg tgacggtttc gccgccaacg aagtggtgcg
      181 cttctatata gaccgatcac agaacagttc cggcaccgag gtaaggtgca ggatcagcat
      241 cagcacccgg ccgctggcga cggccatgcc gaaggtatac gcccccttgg gaaggatcgc
      301 cagccagagg gcaatctgta accagccggc aggggcgtgg tcgtagaagt aggtgtaggg
      361 ggccagcttg cccagccgga tcaccgacca cgcctgctcc atgtagatcc cctcatcgcc
      421 caggtatagg ggatacccga acatgttgaa ggcgtgggag gcgagagcca cgaccatgag
      481 tccccacagc agccccctct cgcccaaccg attcagagcg tcttctaccc gattgtgtcc
      541 catctaggcc gccctctgtc cctgatccgc cctgtgagcc ccaacatgct gagtcttctc
      601 ccagttggaa accccgcgca cctccctgat gaaagccctg aaggccgcgt acgccagcat
      661 ccactggtag gggagatagg ccagcgccat cgcgaccggg gtcttccagg tcgcgtggag
      721 tccgtgggca tcggcgaact cgtacaggcc gatcagcgac atgaagtagt ggctgaccaa
      781 caggtaggcc ggcaacagaa gcagcagcgc ttggcccacc ggcaccttag tggagaacat
      841 catccagaga gatatcggta catagagggc cagcaccgcc tgagcggtgg gaaagcccag
      901 cgtgtaggcg gccatcaccc gctgcttcca gttgggcagt ctggtccagt cgcccttccg
      961 cagcgtctgg aggaagccct ggctccaacg ggtccgctga cgtatgaaat ggccgacggt
     1021 gggcggcgtc tcttcacggg tgacatactg gtcgtcgtag acgattcgca ccggctccga
     1081 catcgctgag atccgcatcc ccacatcggc atcctcggta aggttgtgct catcccatcc
     1141 cccgatctcg tccagcagcg gcctctcgaa gaagaccgtg ttgccgccga gaggggtggc
     1201 ccccaacttg gcatggtagt gaagccgcga cttgaaccag aagaagtact ccaacacgtt
     1261 caacgctgag taccaggagg aatcgtagtt catcaactgt acgccgcact gtacgacccg
     1321 cactccctcc tccagcatca ccgtattgac gatgttgaag atctccggat ggatctcatc
     1381 ctcggcatcg aacaccgtga ccacctcgtt ggtggagtcc cggagcccga tattcaaccc
     1441 atggggcttg ttgacggggg tgtcgttgaa caccacgaga cgaacgttgt ttatgtgctc
     1501 tctcttcagg gccgcaatct cctcatccac cttctcgatg gtgccggcgt cggtgtgctc
     1561 gcagatcacc agcacctcca tcaggcggct cggatagttg gcgtgcacca ccctgccgat
     1621 agtctcctga atcacctctt cctcgtggcg agcagggagc agcaccacca tggacttctc
     1681 gggcactcgg aaagtgtcgg gggcgcgggc gcgctgatac gcctcggggc gatcccaggt
     1741 gtacatcatg atgttctggg ttctactctc gat
//
LOCUS       DATALS010000255         6462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_26384,
            whole genome shotgun sequence.
ACCESSION   DATALS010000255 DATALS010000000
VERSION     DATALS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26384"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2321)
                     /locus_tag="VHS06_05690"
     CDS             complement(<1..2321)
                     /locus_tag="VHS06_05690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2987646.1"
                     /translation="MSTATLVEEARQLRGLVAAALALSLAIIAQGYVAAHDYLFDGLL
                     LYLFALALTYRFIPDIPLQSSPSPRAENAEPVRLRGRSFLVVLAFLLCWATSMLLFHS
                     NSMLTPAWTLYALSVIGAPLSVWLATGRPRCSTAAASSSGYVVALGAVMLLAALVRFV
                     GLGSLPFGLWWDEAYSGLQIQRLMTDPSFRPVYVGGMAQEPSLIWYLTVPFFALFGPG
                     AVALRTAAALGGVLGVLSIYLLGGELFGRRAGIVAAALLAVMSWHLGFSRIAFNAVWS
                     VALDGLAAYFFLRAMRTGSMVRFALAGTAMGIGANMYYTTRLLPLVLLLYLGHRLVVE
                     RTAFFRRHLRGLLLFALVVVVTAGPLIQFALQNTQEFGSRVDQVSILKEVTSRGNLQP
                     LAENLRKHLLMFNYQGDANGRHNLPSAPMLDQVTGALFVLGLLLALRRAYRGPYFFTL
                     AWSVVMLTGGVFSLSFEAPQGLRSIDETLAVALLAALPIVGMWEGLDRVWSGEFRLVP
                     PRGLGHVGLPMAALPVIGLLALIGFLNYDRYFVKQASDPKSWSVFSTAETQVSREINA
                     LGSNYAVHLGPTFTSHPTIEFLTGRRGLPAFDPVAQLPLRDARGVALFLEPNQTEAIS
                     AIRRLYPQAEIRSFSPPSGGDPLLYSVVVRQPDVLRMQGMTASYYANSDWSGEPASTE
                     QIPSTSIELGAGLPAMPFSARWESTLASPTYGRYRFKVEGPTSVNVWLDGSPLEHTND
                     VLEVVMAKGNHSLVISAIVTEATAIRLLWQPPNT"
     gene            2658..2912
                     /locus_tag="VHS06_05695"
     CDS             2658..2912
                     /locus_tag="VHS06_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987647.1"
                     /translation="MARVELHFTHTVVHGLMVVEKEFEEGEIAELISRVDEDLVWSAD
                     LPREDFVVTVYQGKELGVYSDPDVEEDDEEEEEEDDDRDS"
     gene            3163..4953
                     /locus_tag="VHS06_05700"
     CDS             3163..4953
                     /locus_tag="VHS06_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX2987648.1"
                     /translation="MSRHRRLAAPMVILVSLALILVACQAQPSPAPVATSAPAAQGTK
                     APAATGGASAGTAAPAAPSAAEMDPNQILRVNIDGEPPTLDPDMASWDTSIAIIHQVF
                     EGLLKFDKDLNLLPGLAKEVPTVANGGISADGRTYTYKLRSDAKYSDGKPVVAKDIEY
                     SLKRTLDPTLATEYASFYYTVVGAEEYNTSKEKDSAKLQTLRDGVGVKAIDDTTLQVK
                     LKEARASFNDLAALWPMYPVREDLVKSFGTADKPDKWTEDPKNYIGNGPYKITEWVHQ
                     DHITLVPNENYYGNKPKIQKMLLTMVTDAQSEYAAYLNGEREMGKVPTALIEQVQKDP
                     NLSKQLVRSPRLSTFAIQFNLKQKPFDNVKVRQAFGMAMDRESLIANVRKGVGKPAYS
                     WIPPGNPGYQADLGKEYKFDPAKAKQLLADAGFPNGQGLPPITFQFANTRTNPIVAQF
                     AQEQWKTNLGVDVKLEPMEPKAFSEAVNKGQYMMGFYGWNADYPDPDNWLPELFGTGA
                     GNNHTYYSNAKLDDLMKKAIVEPDEKKRLQMWADAQKTVVDDSPIIFLFHDENFVLVK
                     PYVKDAFLTGMDGTALPAQYSTDRIWLAKH"
     gene            5077..5994
                     /locus_tag="VHS06_05705"
     CDS             5077..5994
                     /locus_tag="VHS06_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2987649.1"
                     /translation="MQQYIIRRVLWLIPVLFFISLITFALMHSVPGGPWDREKKLAPS
                     VVENLNARYGLDKPLAEQYLSFVGNALHGDLGVSFSYQDRKVTSVILEGLPVTATLAV
                     SALLVAMLVGIPLGILAALKQNSWADYLCLFFATVGASVPNFVMAMILIIVFSVGLHW
                     LPTNGWGTWQKAVMPALALGLYHAALLARMTRASMIEALRQDFVRTARAKGLVEHLIL
                     FRHIIKNALIPVVTILGPVAAGLIAGSFIIESIFAVPGVGKFFIQGIFARDYGLMMGT
                     TLFYAVVIALANLAVDILYGFIDPRIRYQ"
     gene            6028..>6462
                     /locus_tag="VHS06_05710"
     CDS             6028..>6462
                     /locus_tag="VHS06_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929199.1"
                     /note="with OppABDF is involved in the transport of
                     oligopeptides of up to 5 amino acids into the cell;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HEX2987650.1"
                     /translation="MQSESLALPRRQASLWGDAWRRLIRNRMAVLGGIIVILLLLVAL
                     LADVIAPYSPTKQDYTAIFQTPGSKYLLGTDNLGRDQLSRLIYGARVAVMVGIFTQLV
                     VLAIGLPIGAVAALAGGKVDNLLMRFTDIVYAFPDLLLIILLS"
BASE COUNT         1398 a         1899 c         1981 g         1184 t
ORIGIN      
        1 gtattgggag gctgccacag cagcctgata gccgttgcct cggttacgat ggccgagatg
       61 accagggagt ggttgccctt cgccatcaca acctccagga cgtcgttcgt gtgttccagg
      121 ggggatccgt ccagccagac gttgaccgat gtggggccct ctaccttgaa gcggtacctt
      181 ccgtaggtag gtgaggcgag cgttgactcc catctagctg agaacggcat cgccggaaga
      241 cccgcaccta gctcgatcga agtggatggg atctgttctg tcgatgccgg ctcacccgac
      301 cagtcgctgt tagcgtagta gcttgcggtc atcccctgca tgcgaaggac gtcgggctgt
      361 cggacgacca cagagtagag cagtgggtct ccgccggaag gcggagagaa gctgcggatc
      421 tccgcctgcg ggtagagtcg acggattgcc gatattgcct cagtctgatt gggctccagg
      481 aacaaggcca cgcccctagc atcccgcaga ggcaactggg caaccgggtc gaacgccggc
      541 aggccacgcc gtccagtcag gaactctatg gtcgggtgac tcgtgaaggt ggggccgagg
      601 tggacggcat agttggagcc gagggcgttg atctcgcgac taacctgggt ttctgcggtg
      661 gagaagacgc tccaactctt gggatccgac gcctgcttta cgaagtaccg atcgtagttg
      721 aggaacccta tcagcgccag gaggccgatg acggggagcg ccgccatggg tagacccaca
      781 tgcccgagtc ctcgaggcgg caccagacga aactcaccgg accacaccct gtccaatccc
      841 tcccacatac ccacaatggg cagagctgcc aggagggcta ccgctaacgt ttcgtcgatg
      901 gatcgaagcc cttgcggagc ctcgaaagag agggagaaca cgccgcccgt gagcatgacg
      961 acgctccagg ccagggtgaa gaagtagggg ccgcggtatg ctcttcggag cgccagcagt
     1021 aacccgagga cgaagagcgc gcccgtgacc tggtcgagca tcggagcaga gggaagattg
     1081 tgtcgaccat tggcgtcacc ctgatagttg aacatcagca ggtgcttgcg aaggttctcc
     1141 gccagcggct gcaggttgcc gcgactggtg acctctttca gaatggacac ctgatccacc
     1201 ctggagccga attcctgggt gttctggagg gcgaactgta tgagcggacc ggcggtcacg
     1261 acgacgacga gggcgaagag tagcagccct ctcaggtgac gccggaagaa ggcggtgcgc
     1321 tccacgacta accggtgacc cagatagaga agcagaacga ggggaaggag cctagtcgta
     1381 tagtacatgt tagcgccgat gcccatggct gtgccggcaa gggcgaagcg caccatgctc
     1441 cccgtgcgca tcgcacgcag gaagaagtag gcggccagcc catccagtgc gactgaccag
     1501 accgcgttga aggcgatcct gctgaagccc aggtgccagc tcataactgc tagcagagcc
     1561 gccgcgacga tgccggcccg acggccgaac agctcgccgc ccaggagata gatggacagc
     1621 acgccaagca cccctcccag cgcggctgcc gtcctgagtg ccaccgcgcc ggggccgaac
     1681 agggcgaaga atggcacggt gaggtaccag atcagggagg gctcctgtgc cattcccccc
     1741 acgtacacgg gtctgaagga tggatcggtc atcagacgct ggatctgaag tccagagtac
     1801 gcctcgtccc accagagacc gaagggcagt gagcccaacc caacgaagcg aactagtgca
     1861 gctagcagca tcaccgctcc gagggcgaca acgtagcccg aagaggatgc agccgccgtg
     1921 ctgcagcggg gtctgccagt ggccaaccac acagagagcg gggcgccgat cacactcagg
     1981 gcgtagagtg tccacgccgg ggtgagcatg ctgtttgagt ggaagagcaa catcgaagtg
     2041 gcccaacaga gaaggaatgc caagacgacc aggaagctgc ggcccctcag ccggactggc
     2101 tcggcgtttt ctgcccgtgg ggagggtgac gattgcagcg ggatgtcggg gatgaaccgg
     2161 taggtcaggg cgagtgcgaa caggtacagc aatagtccat cgaagaggta gtcgtgcgcg
     2221 gccacgtagc cctgggcgat aatggccagg gataatgcca acgcggccgc taccaatccc
     2281 ctcaactgcc gcgcctcttc cactaatgtc gccgttgaca tccgcctccc accacccgca
     2341 acagtaggat tggagcactt ggtggaaggt tagagagatt tgcagtccga atcaagggtt
     2401 gaaatggggt gttgcaggtg actatcttgc aggaacgata ggaactcggc cgagactccc
     2461 acctgagcca gtatctcgtc gttgaccggc ctccgcgctc tcatctatga ttcccgcaga
     2521 acatcaactc ggatggtagc tgctgggccg gttgcccatc ggatgaacgg aggagagaag
     2581 tgcaggacct tcactacgat cgtatctgtc gtaccccgcg ctctgaggct tacttgataa
     2641 gccaggggga cgagcccgtg gctcgtgtcg aactccattt cacccataca gtcgtgcacg
     2701 gactgatggt ggtggagaag gagttcgagg aaggggagat tgccgagctt atcagccggg
     2761 ttgatgaaga cctggtctgg agtgcggacc ttcctcgaga agacttcgtc gtgaccgtgt
     2821 accagggcaa ggaactgggc gtgtacagcg acccagacgt cgaggaggac gacgaggaag
     2881 aagaggagga agacgacgat cgcgattcct gaccatcgca accgattgtg tcgcttgcat
     2941 gcaatcgttg cctcaggttg acgggaatta caaaagtgat atgatggggc ccacttgtgg
     3001 tgaaagtgga cagattatct gaagggaccc atagagttgc ccttcgccca gtggagggtg
     3061 ctatggcgag atcccgtatc ccggcagcgc agggcagtgc tgctgcaggg gttgcactgc
     3121 cccaagaatc caccccaact cttcactgat atggaggagc atgtgtctag acatcgaaga
     3181 ctagctgccc ccatggtgat cctggtctcc ttagcgctca ttctggtggc atgccaggcg
     3241 cagccgtcgc ctgcgcctgt ggccacctcg gcgccggcag cccagggaac caaggcccca
     3301 gcagccaccg gcggtgcttc cgccggaaca gcggcacctg ccgccccatc ggcggccgaa
     3361 atggatccca atcagattct ccgagtgaac atcgatggcg agccgcccac cctcgatccg
     3421 gacatggcat cctgggacac ctcaattgcc atcatccacc aggtcttcga gggcttgctg
     3481 aagttcgaca aggacctcaa cctgttgccg gggctggcca aggaagtccc gacggtggcc
     3541 aacggtggaa tctcagccga tggcaggacc tacacctata agctgcgcag cgatgccaag
     3601 tacagcgacg gaaagcccgt ggtggcaaag gacatagagt actcgctgaa gaggactctc
     3661 gacccaactc tggccaccga gtacgcctcc ttctactaca cggtcgtggg cgccgaggag
     3721 tacaacacct ccaaggagaa ggactccgcc aagttgcaga cgctgcgcga cggcgtgggc
     3781 gtcaaggcga tcgatgacac caccctgcag gtcaagttga aggaggccag ggcaagcttc
     3841 aacgacctgg ccgctctgtg gccgatgtac ccggttcgtg aggacctggt caagagtttc
     3901 ggcaccgccg acaagcccga caagtggacc gaggatccca agaactacat cgggaacggt
     3961 ccctacaaga tcacggagtg ggttcatcag gaccacataa ccctggtccc caacgagaac
     4021 tactacggga acaagccgaa gatccagaag atgcttctga ccatggtcac cgacgctcag
     4081 tctgagtatg ccgcctattt gaacggcgag cgcgagatgg gcaaggtgcc gaccgccctg
     4141 atcgagcagg ttcagaagga tcccaacctg agcaagcaat tggtccgcag tccgaggctc
     4201 tcgaccttcg ccattcagtt caacttgaag cagaagccgt tcgacaacgt caaggtgcgg
     4261 caggcattcg gtatggcgat ggaccgcgag tcgttgatcg ctaacgtccg caagggcgtg
     4321 ggcaagcctg cctacagctg gataccaccc ggcaacccgg gctatcaggc tgacctgggc
     4381 aaggagtaca agttcgaccc ggcgaaggcg aagcagttgc tcgccgatgc cggcttcccc
     4441 aacggacagg gactgccgcc catcaccttc cagtttgcca atacccgaac caaccccata
     4501 gttgcgcagt tcgcgcagga gcagtggaag accaacctgg gagtcgacgt gaaactggag
     4561 cctatggagc ccaaggcttt ctcggaagcc gtgaacaagg ggcaatacat gatgggcttc
     4621 tatggctgga acgcggatta cccagatccg gacaactggc tgccggaact gttcggcacg
     4681 ggtgcgggca acaaccacac ctactacagc aacgcgaagc tcgacgacct gatgaagaag
     4741 gctatagtcg agccggacga gaagaagcgc cttcagatgt gggccgacgc gcagaagacg
     4801 gttgtggatg actctccgat catcttcctg ttccacgacg agaacttcgt cctggttaag
     4861 ccctacgtca aggacgcctt cctgaccgga atggacggca cagcccttcc ggctcagtac
     4921 tcgaccgatc gtatctggct ggccaagcac taggactgga caatggcatc cttctggggc
     4981 cggcagcaat gccggcccca gcctctatca ctcgttcgtg aaatgcgggg cgctccggcc
     5041 tcgtactgag cactactcgg caaccagcag gtgaccatgc aacagtacat catcagaaga
     5101 gtgctctggc tcattccggt cctgttcttc atatctctga ttacctttgc cctgatgcac
     5161 tctgtcccag gaggaccgtg ggatagggag aagaagttgg ctccctctgt ggttgagaac
     5221 ctcaacgctc ggtatgggct ggacaaaccg ttggccgagc agtacctcag ttttgtcggt
     5281 aacgcgctgc acggcgacct gggcgtctcc ttcagctatc aggaccggaa ggtcacaagc
     5341 gtgatccttg aaggtctccc cgtaaccgcg acgttggccg tgtcggcgct tctggtagct
     5401 atgctggtcg gtataccgct tgggattctc gctgccttga aacagaactc gtgggccgat
     5461 tacctgtgtc tcttcttcgc aaccgtggga gccagtgtgc cgaatttcgt catggccatg
     5521 atcctgatca tagtattctc tgttgggttg cactggcttc cgaccaacgg atggggaacg
     5581 tggcagaagg ccgtcatgcc cgctctcgca ctggggctct atcacgcggc acttctggcc
     5641 aggatgacaa gggcaagcat gatcgaagcc ttgcgccagg acttcgtgcg taccgcgcgg
     5701 gcaaaagggc tcgtggaaca tctcatcctg ttccggcaca tcatcaagaa tgccttgatc
     5761 ccggtggtga cgatcctcgg cccggtggcc gcaggtctca tagcaggatc gttcatcatc
     5821 gagagcatct tcgccgtccc cggcgtcggc aagttcttca tacagggcat tttcgcgcgg
     5881 gactacgggt tgatgatggg cactaccctc ttctatgcgg tagtgatagc cctcgccaat
     5941 ctggcagtgg acatcctgta cggcttcata gatccgcgca tcaggtacca atagaggagc
     6001 ctatccttga cagccgctgt cactacaatg cagtccgaat cacttgcact acctcgccgt
     6061 caggcaagcc tgtggggaga cgcctggcga cgtttgatcc gcaaccgcat ggccgtcttg
     6121 ggcggcataa tcgtgatctt gcttctcctc gtcgcgctgc tggcggatgt gatcgctcca
     6181 tacagcccga ccaagcagga ctatacggcc atcttccaga ccccaggcag caagtacctg
     6241 ctggggacag acaacctcgg tagggaccaa ctatccaggc tgatctacgg ggccagggtc
     6301 gcggtgatgg tgggaatctt cactcaactc gttgtactgg ccattggctt gcccatcggc
     6361 gccgtcgctg cactggccgg gggaaaggtg gacaacctcc tgatgcgttt cacggacatc
     6421 gtctacgcgt tccccgatct gctgctgatc atcctcctgt cg
//
LOCUS       DATALS010000256         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_250382, whole genome shotgun sequence.
ACCESSION   DATALS010000256 DATALS010000000
VERSION     DATALS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250382"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VHS06_05715"
     CDS             complement(<1..852)
                     /locus_tag="VHS06_05715"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2987651.1"
                     /translation="MKHLIVGNGPAGISAARTLRRLAPGDQVALVAEESQPFYSKVLT
                     SYFVGGKVPYENMLLSNAEGFQEKGIQLLMGKQAVRIDPATRELLLSGGERVSCDRLL
                     LATGASPFIPPIEGIKLPGVFTLWTHDDSVKMKEWIQEGQHAVVVGAGLVGLKAAEAF
                     LMRGLTVTVVELLDRVLPQVLSLEDAAIVKRELEKRGVVIRTSTGVSQISGEDRVREV
                     TLNDGSTLPCDVVVVATGVRPNLSLAKSAGISFGRGILVGEFMETSVPGIYAAGDVTE
                     AYDLARGR"
     gene            complement(941..1591)
                     /locus_tag="VHS06_05720"
     CDS             complement(941..1591)
                     /locus_tag="VHS06_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987652.1"
                     /translation="MTHSLHRRGDVESLKKDFLILSCSAKGFNDGWYGPLGSEFVRIC
                     TSHKPVNGGDMKTGNLLYVDPEEIANKVTNTTILEFTFDNKQNVIDCINELKARDLGI
                     SVVISGMIEEVNDIVEKAGLPKVHTREFSLGTHGKTRLLPEWEVLEFTTMCGHAMVAS
                     ESVKKAIVDARKGHTSPRAAAVQMGKCCSCGNFNVNRAVELMEKNAPLWTADYPVY"
     gene            complement(1588..2337)
                     /locus_tag="VHS06_05725"
     CDS             complement(1588..2337)
                     /locus_tag="VHS06_05725"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2987653.1"
                     /translation="MVIDETKCIGCGKCLPFCPVQARAFGRAANGGRTFVVIDKEKCI
                     ECGVCERAGVCPVDAMHMEELPWPRMVRGILSNPLLEFKATGVPGRGTEEIKTNEITG
                     RVKHGRVGVGIEMGRPGISARWSEVEKVCMAVAKMGIAFCEGNPITHFMADKKTGKLD
                     PAVLNEASLSAIVEFEMAEDQIPELFRILRDEVNDQINTVYTFEIAARLYPDGTTPVT
                     KLIEDGGYQMLKVSKNNLGLGRPRFQEEVSA"
     gene            complement(2487..>2807)
                     /locus_tag="VHS06_05730"
     CDS             complement(2487..>2807)
                     /locus_tag="VHS06_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987654.1"
                     /translation="PAVLDAGSCWEMPAALEMAKLFKEGLKLPLAAAMPELSRPASWC
                     SALAIASQGVPTYVGPVLPLDGGLETVGALNEMLKGKGGALVGPGQIGDPEAVVNLIT
                     GARS"
BASE COUNT          452 a          933 c          847 g          575 t
ORIGIN      
        1 tctaccccgg gccaggtcgt acgcctcggt gacgtcgccg gcggcataga tccccggcac
       61 gctggtttcc atgaactcgc ccaccaggat cccccgccca aaggagatcc cggccgactt
      121 cgccagggag aggttgggac gtaccccggt ggcgacgacc accacatcac acggcagggt
      181 ggagccgtcg ttgagggtca cctctcgcac tctatcctcc ccggagatct ggctgacccc
      241 cgtggaggtt cggattacga ctcccctctt ctccagctcc cgcttgacga tggccgcgtc
      301 ctccaggctc agcacctggg gtagcacacg gtccagtagc tccaccacgg tgactgtcag
      361 ccccctcatc aggaaggctt cggccgcctt cagccccacc agtccggccc caacgaccac
      421 ggcgtgttgt ccttcctgga tccactcctt catcttgacc gagtcgtcgt gggtccagag
      481 ggtaaacact ccgggcagct ttataccctc tatggggggt atgaagggcg aggccccggt
      541 ggccagcagc agccggtcgc agcttacccg ttcaccaccg gagagcagaa gctctcgggt
      601 ggccggatcg atcctcaccg cctgctttcc catcagcagc tggatcccct tttcctggaa
      661 tccctccgca ttggagagca gcatattctc gtagggcacc ttgccgccga cgaagtagga
      721 ggtgagcacc ttggagtaga agggctggga ctcttcggcc accagcgcca cctgatcccc
      781 cggagccaac cttcgcaggg ttctggcagc gctgataccc gccggtccgt tgcccactat
      841 cagatgtttc atgctcgtat cccttcgtgg gggttgggtg ttgggggatg ggggttggga
      901 attcttctgc ccccaacccc tatcccctat cccctatccc ctagtagact gggtagtcgg
      961 cggtccagag cggcgcgttc ttctccatca gttccacggc ccggttgacg ttgaagttgc
     1021 cacaggagca gcactttccc atctggaccg cggccgccct gggcgaggtg tgtcccttcc
     1081 tcgcatccac gatcgccttc ttgacggact cagaggccac catggcgtgt ccgcacatgg
     1141 tggtgaactc cagcacctcc cactcgggca gcagcctggt cttgccgtgg gtgcccagcg
     1201 agaactctcg ggtgtggacc ttgggaaggc cggccttctc gacgatgtcg ttgacctctt
     1261 cgatcatgcc ggagatcacg acggagatcc ccagatcccg cgctttcagc tcattgatgc
     1321 agtcgatgac gttctgcttg ttgtcgaagg tgaactccag gatggtcgtg ttggtgacct
     1381 tattggcgat ctcctcggga tcgacgtaca gcaggttgcc ggtcttcatg tcgccgccgt
     1441 tgaccggctt gtggctggtg cagatccgca cgaactccga cccgagggga ccgtaccagc
     1501 cgtcgttgaa acccttggca gagcaggaga ggatcaggaa gtccttcttc aggctctcga
     1561 cgtcgcctcg gcgatgcaga ctgtgagtca tgctgacacc tcctcctgga agcggggccg
     1621 gccaaggccg aggttgttct tgctgacctt gagcatctga tagccgccgt cctcgatcag
     1681 cttggtgacc ggggtcgtgc catccgggta gaggcgggcg gctatctcga aggtgtagac
     1741 cgtgttgatc tggtcgttaa cctcgtcccg aaggatgcgg aacagctccg ggatctggtc
     1801 ttcagccatc tcgaactcga ctatggccga gagggatgcc tcgttcaaca cggcggggtc
     1861 gagcttgccg gtcttcttat cggccatgaa gtgggtgatg gggttgccct cgcagaaggc
     1921 tatgcccatc ttggcgacgg ccatgcacac cttttccacc tcggaccacc tggcactgat
     1981 gcccgggcgg cccatctcga tgcccacgcc gacccggccg tgcttgaccc ggccggtgat
     2041 ctcgttggtc ttgatctcct cggtgcctcg gccgggaacg ccggtggcct tgaactcgag
     2101 gagtgggttg gagaggatgc ccctgaccat ccgtggccag ggaagctcct ccatgtgcat
     2161 ggcatccact gggcagacgc cggcgcgctc gcagacgccg cactcgatgc acttctcttt
     2221 atctatgacc acgaatgttc gccccccgtt cgccgctcga ccgaaagcgc gggcctgcac
     2281 gggacagaag gggaggcact tgccgcagcc tatgcatttt gtctcgtcaa taaccattta
     2341 ggctacctct tatctgtagg ggcggaccta tgtgtccgcc ctgggcgcac acgcaggtgc
     2401 gcccctaccg ttggatagcg gttgtccgcc cttggcctgg acgcacgtgg aactgcgccc
     2461 ctaccgttgg tggcggttgt ccgcccctac gacctcgcgc ccgtgatgag gttgacgacg
     2521 gcctcgggat caccgatctg gcccggcccc acgagggcgc cgcccttgcc cttcaacatc
     2581 tcgttcaggg cgccgacggt ctccaggccg ccatccagcg gcaaaacggg tccgacgtag
     2641 gtgggcaccc cctgggaggc gatggccagg gcgctgcacc agcttgcggg cctggacagt
     2701 tcgggcatcg ccgctgctag aggcagcttc agcccttcct tgaacagctt tgccatctcc
     2761 agcgccgccg gcatctccca gcagcttccg gcgtccagta ccgcggg
//
LOCUS       DATALS010000257         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_160397, whole genome shotgun sequence.
ACCESSION   DATALS010000257 DATALS010000000
VERSION     DATALS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160397"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2124
                     /locus_tag="VHS06_05735"
     CDS             <1..>2124
                     /locus_tag="VHS06_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2987655.1"
                     /translation="LPGPVALAILDINGKEIARSDDAPLGLPDPAGSSLLTDARHSLS
                     ISTALTDSTSGRAPVVAFGARMVDQTGHFSGMVVVRADASLLSLMLSHATSNAEEKRF
                     LVDGQGRLLARTVVGASSLEDLSGLPPVAALMAGEGAAGSVSYTGEDGEWLAGYSRVP
                     GFGWGIVVEQRKDYALGSVRAGRDLASRILLLAIVLSIVVGSVAAGLLTTQLRVFTEA
                     VERLGIGDLNAPIPYSGISEFSRLATVFRTMRERLAFRTAESERAVKALRARERQQAA
                     VAQVGQWALAGTEIQTLFDGVVALVAITLNVPFAQLLELRPHGRSLVLRASVGMEDEM
                     ASRLEIPSGDASQAGYTLMADEPVIVEDLSAETRFSCPPIYSQHGLVSGVSVLVSGRE
                     RHFGVLSAHTVARRAFTRDDVHFLQSIANHLAMAIDRMRSEEARNFLSEASGLLASSL
                     DYETTLESVVRLAVPRLADWCSVDVVDDAGLPQQVAVAHVNPAKERLLEELRANPRLG
                     DWSQHPVLKVVRSGLPLLFDEIPDAMIDGMGKGSIDPNTIREIGMKSAAILPLRARDR
                     TLGAITLVSTGTSHRYGQAEMLLAEELARRCAIAVDNTLLYQESREAVRARDEFLSVA
                     AHELKTPVTSLRGYAQLSLYQMEREGRLNEDRAVRAFQVIDAQSQKRGEKLHEKEGEP
                     RNEKGRPIPGEIRKRDPVEIRGKDPG"
BASE COUNT          396 a          605 c          720 g          403 t
ORIGIN      
        1 cctgccgggc ccagtcgcgc tggcgatcct ggatatcaat ggaaaggaga ttgcccgcag
       61 cgacgatgcc cccctcggtc tgcccgaccc ggcgggaagc agccttctca ccgacgctcg
      121 gcatagtctc agcatctcga ccgcgttgac ggattcgacc tcggggcgag cgccggtggt
      181 ggcattcggt gcaaggatgg tggaccagac cgggcacttt tccggcatgg tagtagtacg
      241 cgcggacgcc agcctgcttt cattgatgtt gtctcatgcg acgtcgaatg ccgaggagaa
      301 gcggttcctg gtggacggcc aagggcgcct gctcgcccgg actgtggtgg gggcgtcatc
      361 gctcgaggat ctctcgggtc tacctccagt ggctgcactg atggctgggg aaggagcggc
      421 ggggtcggtt tcatacaccg gcgaggacgg cgaatggctg gccggatatt cacgcgtccc
      481 cggttttggc tgggggattg tagtagagca gcgtaaggac tacgcgttgg gctccgttcg
      541 tgcaggccgg gacctcgcct ccaggatact gctgctggcg atcgtcctat ccatcgtggt
      601 tggatccgtg gccgccggcc tgttgaccac ccaactgcgt gtctttacgg aggccgtgga
      661 gcggctgggc atcggggatc tgaacgctcc catcccttac tccggcatct ccgagttctc
      721 tcgactggcg acggtgttca ggacgatgcg agaaaggctt gcattcagaa cggctgagag
      781 tgagcgggcc gtgaaggcgc tgagggcgag ggaacgacaa caggccgcgg tcgcccaggt
      841 cggacagtgg gcgctggcgg gcacagagat ccaaacgctg ttcgacggag tagtagcact
      901 ggttgcaatc acactgaacg tcccatttgc tcaattgctg gagttgcggc ctcacggtcg
      961 gtcgttggtg cttcgcgcgt cggtgggtat ggaggacgag atggcgtctc gcctcgagat
     1021 cccttcaggg gatgcctctc aggccggtta caccttgatg gcggacgaac ctgtgatagt
     1081 cgaggatctt tccgcggaga ctcgcttctc ctgtcctccg atctacagcc agcatgggct
     1141 ggtgagtggc gtgagcgtgc tggtgtctgg gcgggagcgc cactttggcg ttctgagtgc
     1201 acatacggtc gctcgtcgtg ccttcactcg agacgacgtc cacttcctgc agtcgatcgc
     1261 gaaccacctg gcgatggcga tcgatcgcat gcgctcggaa gaggcccgga actttctctc
     1321 ggaagccagc gggctgctgg cctcatcgtt ggactacgag acgactctcg aaagcgtggt
     1381 acggttggcc gtgccacgcc ttgccgactg gtgcagcgtg gacgtcgtgg atgatgccgg
     1441 tttaccccaa caggtcgccg tggcccacgt gaaccctgcc aaagagcggc tactagagga
     1501 gttgcgcgcg aatccccgcc tcggcgattg gtcgcaacac ccggtgctga aggtagtgcg
     1561 cagcgggcta ccgctgcttt tcgacgagat cccagacgcc atgatagatg gcatggggaa
     1621 agggagtatc gacccgaaca ccatccgaga gatagggatg aagtcggcgg caatcctgcc
     1681 gctgcgagcg agggaccgca cactcggggc gataaccctg gtttccacag gcacaagcca
     1741 tcgctacggg caggcagaga tgctgctggc cgaggagttg gcacgccgat gtgcgatagc
     1801 ggtggacaat accctgctct atcaggagtc gcgcgaggcg gttcgtgctc gagacgagtt
     1861 cctgtcggta gccgcccatg agttgaagac acctgttacg agtctcaggg gctacgccca
     1921 actctccctc tatcagatgg aacgggaagg caggctgaac gaggatcgcg ctgtgcgtgc
     1981 cttccaggta atcgatgctc agtcgcagaa gcgaggggaa aagctgcacg aaaaggaggg
     2041 cgagccccga aacgagaaag gccgccccat accaggagag attcggaagc gagatccggt
     2101 agaaatccgc ggtaaagacc ccgg
//
LOCUS       DATALS010000258         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_327335, whole genome shotgun sequence.
ACCESSION   DATALS010000258 DATALS010000000
VERSION     DATALS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327335"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..338)
                     /locus_tag="VHS06_05740"
     CDS             complement(15..338)
                     /locus_tag="VHS06_05740"
                     /inference="COORDINATES: protein motif:HMM:NF025696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2987656.1"
                     /translation="MAVPDFQSITLPLLQLMSDGAEHALWETVEPLSNEFKLTGEERR
                     ELIPWSGERRFDNGVLWAATYLSQSGLLEMTEPGRYRITARGREVLAEKPERVDLKYL
                     ERFRD"
     gene            complement(553..1020)
                     /locus_tag="VHS06_05745"
     CDS             complement(553..1020)
                     /locus_tag="VHS06_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987657.1"
                     /translation="MKRLLMPLLILALLLAVASATLTACGGSSTSSADRVFAEKVMKM
                     WSSNDAATAKQLFAENATIYWPEDAAEAPVKGIDAIASTIASYPLDPVLMGDSVYTFV
                     PSTEDMKLLASTYKDSRFIAFPAALGNDAFLTWLELKNGKIQNEWISYMYPFK"
     gene            complement(1092..>2381)
                     /locus_tag="VHS06_05750"
     CDS             complement(1092..>2381)
                     /locus_tag="VHS06_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX2987658.1"
                     /translation="GSRSSRLRVAAYACAATVLVAALTAASYEALQHLGGDQNVVVIT
                     DDTASARTPGQTVSQAPESTAAIYRGNLERTGVYPSGGPTGPPELLWKSKLPYADRDD
                     WVPPPSPVISEGVLYIGAHYSLYAFDAQSGRQKWEFPTASWVRTSPAVAGGVVYFWSE
                     DTYLYALDARSGQEKWRHLIKGVGCTLAVSDGVVYFGGLGAFDAQTGQPLWGVEAGVA
                     FSLSIAGDVAYVGCSPYGFSAFMGLYTVDLDGEQPPSQSSVTRWANSGLDLPLAIRGS
                     VAIVDGVAYYASVYENSFDAVELRTLQRKWSFRTGDVHRPDPGLDHGAAIVSPAVAGG
                     LLYGGSEGGPLYALDIENGRERWRFAMDITQMSSPAVSGQVLYVGSKDGYLYALNATT
                     GKQDWSFKTGDAISTSPAIADGVVYFASDDGYLYAVR"
BASE COUNT          456 a          734 c          731 g          460 t
ORIGIN      
        1 tggcacatcc ataatcaatc ccggaatcgt tccaggtact tcagatcgac gcgctcaggc
       61 ttttcagcca gcacttcgcg tccccgtgca gtgatccgat atcttccagg ctctgtcatc
      121 tcgagaagac ccgattggga caaataggtc gcggcccaga gcacaccgtt gtcaaaccgg
      181 cgttccccac tccaggggat cagctctctc cgctcttctc ctgtgagctt gaattcgttg
      241 gacaacggct cgaccgtctc ccaaagagcg tgctcggctc catcgctcat caattggaga
      301 agggggagtg tgattgactg gaaatcagga accgccatct cccccaccct cgttagcatc
      361 ccgattgcag ctcagtcatg tgtgaaacgt gacgacgtac acgtgacgga tggtagtaca
      421 cggatcagcc gcgttccagg gatgaggtac aagagggcag gggtgcgggg tcattctcaa
      481 gaagtggaac ggtcaggcac tcatgtgcaa atcgctgcct ctggctgggt tagtatcgct
      541 attacgaccg atctacttga acggatacat gtaggagatc cattcgttct ggatcttgcc
      601 atttttgagt tccagccagg tcaagaaagc gtcattcccc agcgccgccg ggaaggcgat
      661 gaaacgtgaa tccttgtagg tcgaagccag aagcttcatg tcctcggtcg aagggacaaa
      721 ggtgtagacg ctgtcgccca tgagaacggg atcgagagga tagctcgcga tggtcgatgc
      781 gatggcgtcg atgcccttga caggggcctc ggcggcgtct tccggccagt agatcgtggc
      841 gttctcggcg aacagttgct tggcggtggc ggcatcgttc gaggaccaca tcttcatgac
      901 cttctcggcg aagacccggt cggcactgct cgtgctgctc ccgccgcagg ctgtgagcgt
      961 ggccgatgcc acggctagga gcagggcgag gatcagcagc ggcatcagta gacgtttcat
     1021 ggttcctccc ttcgttccca cacgtggctg caaaaaagca cgagccgtga gctgttcgag
     1081 agccctgcgg cctagcgcac cgcgtagagg tagccgtcgt cactggcgaa atagacgact
     1141 ccgtcagcga tggccggcga cgtgctgatc gcatctccag tcttgaaact ccagtcctgc
     1201 ttcccggtgg tggcattcag cgcgtataga tagccgtctt tgctgccgac gtagagcacc
     1261 tgccccgaga cggccgggga ggacatctgg gtgatgtcca ttgcgaacct ccaccgctcc
     1321 cgcccgttct cgatgtccag cgcgtagagg gggccgccct cgctgccgcc atagagcagt
     1381 ccgcccgcca ccgctgggga gactatcgcc gctccgtgat cgaggcctgg atcggggcgg
     1441 tgcacgtcgc ccgtccggaa gctccatttc ctctgaaggg tccggagttc cacggcgtcg
     1501 aagctatttt cgtagacaga ggcgtagtag gccactccgt ccacgatggc caccgagcct
     1561 ctgatcgcca gcggcaagtc gagcccggag ttggcccacc gggtgaccga actctgggag
     1621 gggggttgct caccgtcgag gtcgaccgta tacagaccca tgaacgcgga gaacccgtag
     1681 gggctgcacc cgacatacgc gacgtcgcct gcgatggata gggagaaagc aacccctgcc
     1741 tccacgcccc acagtggttg gccggtctgg gcgtcgaagg ccccgagccc accgaagtag
     1801 accacgccgt cgctgacggc aagggtgcat ccaaccccct tgatgagatg cctccacttc
     1861 tcctgtccgc ttcgcgcgtc caaggcgtag agatacgtgt cttcactcca gaagtacacc
     1921 acccccccag ccacggccgg ggaggtgcgc acccatgagg ccgtcgggaa ctcccatttc
     1981 tgccggccgc tctgcgcgtc gaacgcatag agggaatagt gcgcgccgat gtaaaggact
     2041 ccctcggaga tgaccgggga gggtgggggt acccagtcat ccctatctgc gtacggcaac
     2101 ttggacttcc acagcagttc gggtggtccg gtcggtccgc cgctcgggta cacccctgtt
     2161 cgctcgagat tgccccggta gatggcggcc gtgctttcgg gggcctgact gacggtctga
     2221 ccgggtgtcc gagcactggc cgtgtcgtca gtgatgacca cgacgttttg gtctccgccc
     2281 aggtgctgca gcgcctcata actagcggca gtcagggcag ccacaaggac cgtcgccgcg
     2341 caagcatagg cagcgacgcg taggcggctg ctccgagaac c
//
LOCUS       DATALS010000259         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_57237,
            whole genome shotgun sequence.
ACCESSION   DATALS010000259 DATALS010000000
VERSION     DATALS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57237"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VHS06_05755"
     CDS             <1..553
                     /locus_tag="VHS06_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HEX2987659.1"
                     /translation="ALAVADRLLPRKGHELLAVDIGTGSGAVAIAIAACRPSVRVLAI
                     DSSAAALEVAHSNVDRHGLSDRVMLRQGDLLQGVAERVDLLVANLPYIPSSDIDGLMP
                     DVRDFEPRSALDGGPDGTVPIRATLEQAATRMERPGALLFEIGDGQGDALIQAAAAIY
                     PDANVRVDRDYAGFERILAVELP"
     gene            712..1380
                     /locus_tag="VHS06_05760"
     CDS             712..1380
                     /locus_tag="VHS06_05760"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX2987660.1"
                     /translation="MAETKRAEVVRWPEDTAGQRKLIQRAADLIRAGEVVAFPTDTVY
                     GIGVHAWNHAAIQRLYAVKERPATKAIALLISGHDQVRAVSDYSSADLGRLATRFWPG
                     GLTLILPWKPQAQSAAQQPFATVGLRMPDHPIALALIAEVGVPLATTSANLSGAPSAV
                     TAGEVDRQIGDRVAMIIDGGTCPGGKDSTVVDLTTRPPVVRRLGAVSVEELESVLGTV
                     AVET"
     gene            1438..1908
                     /gene="rpiB"
                     /locus_tag="VHS06_05765"
     CDS             1438..1908
                     /gene="rpiB"
                     /locus_tag="VHS06_05765"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016740177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX2987661.1"
                     /translation="MKIAIGSDHAGLTLKKEIIALLKDLNVKVQDFGGSDTWPVDYPD
                     VARPVAEAVAQGEFDRGILICGTGIGMSITANKVKGIRAALCHDTFSAHASREHNDAN
                     VLCMGQRVIGVGPALDIVKTWIATPFSSEERHARRVHKIEEPDAEEVGSDAQSE"
     gene            1969..2223
                     /locus_tag="VHS06_05770"
     CDS             1969..2223
                     /locus_tag="VHS06_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987662.1"
                     /translation="MAILNLRYEALPDEVQNNLTPEQFELAIPDIVAEGETIPETTQY
                     IDLASGVKLIHTRGERAPGNLLPTHDLSGARGKDDSQWQS"
BASE COUNT          454 a          672 c          747 g          399 t
ORIGIN      
        1 ggccctggca gtagccgaca ggttgctgcc gcggaagggg cacgaacttc tcgccgtaga
       61 cattggcacg gggtcgggtg cagttgccat cgccatagcg gcctgtcgcc cttccgttcg
      121 ggtgctcgcg atcgatagct ccgccgctgc attggaagtc gcccactcca acgtcgaccg
      181 ccacggtctc tctgatcgtg tgatgctcag gcagggggat ctcctgcagg gtgttgccga
      241 gcgagtggac ctgctggtag cgaaccttcc ctacatacct tcgtccgata tcgatgggct
      301 gatgccggac gtgcgggatt tcgagccgag gtcggcgctc gatggcggcc cggatggaac
      361 ggttcccatt cgtgccacgt tggagcaggc agcaaccagg atggaacggc ccggtgccct
      421 cctattcgag ataggggatg gacagggcga tgcgttgatt caggcggccg ctgcgatcta
      481 cccggatgcg aacgtccgag tggacaggga ctatgcgggc tttgagcgca tcctggcagt
      541 cgaattgccg tagtgttcga acaggacgtg tggcatttct ctaggagtgg gggtcgtccc
      601 tgacccccac gggctcctat gcgaccgcct gggtcaggga cgacccaggc tcctagaaga
      661 ggagcagtcc aggcgcggat agtacagcat catctcgtgt gggttacatt gatggcagag
      721 acaaagagag cagaggtagt ccgctggccc gaagacactg ccggccagcg taagctgata
      781 cagagggcgg ccgacctcat ccgagccggc gaggtggtcg cgtttcccac cgacaccgtc
      841 tatggtatcg gtgtccacgc gtggaaccac gccgccatcc aaaggctgta cgcagtcaaa
      901 gagcgaccgg cgaccaaggc gatcgccctt ctcatatccg gccacgacca ggtcagggcg
      961 gtgtccgact actcctcggc cgatctcggc cggctggcga ctcgtttctg gcctggaggg
     1021 ttgacactca tccttccctg gaaaccgcag gcacagagcg ctgcacagca accttttgcc
     1081 acggtcgggc tgcggatgcc ggatcaccct atagcgctgg cactgattgc ggaggtcggc
     1141 gtcccgctgg ccactacaag tgccaatctg tccggggcac caagcgcggt aaccgccggc
     1201 gaggtggatc gccagatcgg tgatagggtg gccatgataa tcgacggtgg aacctgcccg
     1261 ggaggcaagg actccacggt ggtagacctg acaacccggc cgccggtggt cagacggttg
     1321 ggagccgtgt cggtggaaga actggagtca gtcctcggca cggttgcggt ggagacatga
     1381 gccgcctgct tcaggaaccc gtccgcaaca gctgtttgtt tgaaaggaga acgcgacgtg
     1441 aagattgcca tcggtagcga ccatgccggc ttgaccctaa agaaggagat catcgccctc
     1501 ctgaaggatc tcaacgtaaa agtacaggac ttcggcgggt cggacacctg gccggtggac
     1561 tatcccgacg ttgcgaggcc tgttgccgag gcagtggcgc agggggagtt cgatcgcggg
     1621 atcctcatct gcggcaccgg gatcggcatg agcataacgg ccaacaaggt gaaggggatc
     1681 cgggccgcgc tctgtcacga caccttcagc gcgcacgcct cccgtgagca caacgacgca
     1741 aacgtcctct gcatgggaca gagggtcatt ggcgttgggc cggcactgga catagtcaag
     1801 acctggatcg cgacgccctt cagcagcgag gagaggcacg cccggcgcgt tcacaagatc
     1861 gaagagccgg acgccgaaga agtgggttcg gatgcccagt ccgagtaggc tgggcacgaa
     1921 tcctgccgcc cccgataccc cttgtcaaca agctggaggt atcgaagaat ggcgatactg
     1981 aacttgcgct acgaagcgct gccggacgag gtccagaaca atctcactcc ggaacaattc
     2041 gagttggcga ttccggacat cgtggctgag ggggagacta ttcccgagac cacgcaatac
     2101 atcgacctgg cctctggtgt aaagctcatt cacacccggg gcgaaagggc acctggtaac
     2161 ctgttgccca cccacgacct ctccggtgca cggggcaaag acgactctca gtggcagagt
     2221 tgaaagcgac cacgggcggg tcgggggcga cccgccctac gtgtgtccgg ta
//
LOCUS       DATALS010000260         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_250583, whole genome shotgun sequence.
ACCESSION   DATALS010000260 DATALS010000000
VERSION     DATALS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250583"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VHS06_05775"
     CDS             complement(<1..821)
                     /locus_tag="VHS06_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987663.1"
                     /translation="MPAFSDNPDPSVIRFLPDPAKADDPHIGELLKGIAANIKEMEER
                     NRDLEEQNNQLLAALRHAHERIHAFEHASVANPELTRLALTTAAQLIESARESIEAAQ
                     KAVKESNADIYEKTIDQVIDYEKRSLTLLENARREADRRKQEVEHNAMEVFLQARDSV
                     LSMARDLETTEVGLDPDRIKSVMESSLTHSGHDKFNEVERLEPSGHSIELTASPFRTF
                     AAISAFHKSILRLPETLDWMARLPWGRGRPRTPRFALRMARAPLFVARASPSWQV"
     gene            1135..2142
                     /locus_tag="VHS06_05780"
     CDS             1135..2142
                     /locus_tag="VHS06_05780"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2987664.1"
                     /translation="MGLLPGLIISGGCLALLLWNVDWSGLLAALKGANYWWLSASLVS
                     LLLAMGFKVLKWQQLLAPVRDCSRLKLFYSIFVGYLINTVLPGRLGEVARAYLLARLE
                     HIGLAVVLSSVAVDRVLDITVLALMLGGVLPLIDLPEWVAASGIGMGSVGLLLLLLCV
                     LLAMPSGNGFFFAMLDAIPRFPGKPVLRKLAAELILGVAGLKGVRQFVAVSFTSVGIW
                     LATALSFYFGQLAFHIQAPMESALLVTSLTSLGMVVPSSPSYLGVFHYLTVLAMVAYG
                     VDKEVALGFAIVMHLQQTLLRGTLGAFSLWRCGLTLMSWRELPVDLESEASVPSRTNL
                     S"
     gene            2367..>2744
                     /locus_tag="VHS06_05785"
     CDS             2367..>2744
                     /locus_tag="VHS06_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987665.1"
                     /translation="MRYTVAAAIAAALFAFVFQAGVVSADPVPTFQLGFLQLASVIPT
                     VVGQPLEQERYDPIRGDALQRTSSGLLVWRKADNWTAFTDGATTWIYGPNGVESRPND
                     ERFSWEQDGGAVVAGAPPAAPPVF"
BASE COUNT          434 a          793 c          864 g          653 t
ORIGIN      
        1 tacacctgcc acgacggaga cgcccgagcc acgaacagag gtgcccgagc catccgcagc
       61 gcaaatcgag gcgtccgggg tcgacctcgg ccccagggca atcgtgccat ccaatccaga
      121 gtctcgggca gacgaagtat cgatttgtgg aaggcgctga ttgcggcgaa ggtgcggaat
      181 ggtgaggcag tcagctcgat cgagtgaccg gatggctcca atcgctcgac ctcgttgaac
      241 ttatcgtgcc cactgtgtgt aaggctggac tccatcacgg acttgatccg atcgggatcg
      301 agtccgacct cagttgtctc caggtcccga gccatggata gaacggagtc cctcgcctgg
      361 aggaagactt ccatggcgtt gtgctcgacc tcttgcttgc ggcggtcggc ttctcgtcga
      421 gcgttctcca acagtgtcag cgatcgcttt tcgtagtcga taacctggtc gattgtcttc
      481 tcgtagatgt cggcgttgct ctccttgact gctttctggg cagcttcaat cgactcccgg
      541 gccgattcga tcaactgtgc ggcagtagtg agggccagcc tggtgagctc tggattggcc
      601 accgaggcgt gctcgaaagc atggatcctt tcgtgggcgt gtctcagcgc agccagcagc
      661 tggttgttct gttcctcgag gtcgcggttc ctctcctcca tctccttgat gtttgcggcg
      721 atccccttca gcagttcgcc gatgtggggg tcgtctgcct tagctggatc agggaggaat
      781 cggatgacac tggggtcagg gttgtcagag aaggcgggca tagctcactt ccgacctatc
      841 tgggcctggg tttctgaatg gttgatcaac gcgacagaag ccgcgcccga cacgccgata
      901 ggaatgacca ttatacatag gcccatagta tcattccaga cactgaagcg ggcggatcct
      961 gcctcagcat acttgcacca gatcgatgct cgatcagtcc tcggtacggc tgctgcatct
     1021 ggatagcccc gcgtgcaaca atgctggctg ccgttctggt ttagcatgta tcgtaacgcg
     1081 aggtttccga tattgactgg tagtggacga gcacgtcctg gctggatgag acacttgggg
     1141 ttacttcccg gactgatcat cagtggcggg tgcctggccc tgctgttgtg gaacgtggat
     1201 tggagcggac tcctggctgc gctgaagggt gcgaactact ggtggctctc tgcgtcgctg
     1261 gttagcctgc tgctggcgat ggggttcaag gtgttgaagt ggcaacagtt gctggcgccc
     1321 gtccgcgact gctcgcgtct gaagctgttc tacagcatat tcgttggtta cctgatcaat
     1381 accgttctcc ccggccgcct cggggaggtg gcgagggcgt acctgctggc tcggttggag
     1441 cacatcggtt tggcggtggt gctctcctcg gtggccgtgg accgggtact ggacataact
     1501 gtgctggcct tgatgctcgg cggtgtgttg cccctcatcg acctgccaga gtgggttgcc
     1561 gcttcgggca ttgggatggg ttccgtagga ttactcttgc tcttgctttg tgtcttgctg
     1621 gcgatgccgt cgggcaacgg cttctttttc gcgatgctag acgccatccc ccgattcccg
     1681 ggcaagccgg ttcttcgcaa actggccgcg gagttgattc tcggcgtagc cgggctcaag
     1741 ggagtcagac agtttgtggc agtgagcttc accagtgttg gcatctggtt ggccacggcg
     1801 ctctccttct actttgggca gcttgccttc cacattcagg cgcccatgga gtctgccctg
     1861 ctggtgacgt ccctcaccag ccttggaatg gtggttcctt cgtcgcccag ctacctgggg
     1921 gtgttccact acctgacggt gctggcaatg gtggcctacg gagtggacaa ggaggttgcc
     1981 ctcgggttcg ccatcgtgat gcaccttcag cagacgctgc tgaggggtac cctgggtgcg
     2041 ttctccctct ggcgatgcgg cctcacgttg atgagttggc gtgaactacc agtcgatctg
     2101 gaatcggaag cgtcggtccc ctcccgtaca aatctgtcct aaccggaagt tgccgccgtc
     2161 tcctgcggcg acttcactcc ctctctcccc tcccccgggt tgcggtccat tttctgcctt
     2221 ttcttcttga tgggctgagc cgagtagatg gcattcctgc tccgtgcggg gcctttcggc
     2281 agggggtccc atccccggtg tcccacatga gggaagctgc gtgaaccggg ttgtggtgtc
     2341 gctgttagca tctggggagg gaccaggtga ggtacacagt cgcggccgca attgcggcag
     2401 ccctgtttgc gttcgttttt caggccggcg tggtctccgc ggaccccgta cccacttttc
     2461 agctggggtt cctgcaactc gcttctgtga tccctacggt cgtcgggcaa cccttggagc
     2521 aagagcgcta cgatccgatc cgcggtgatg ccctgcagcg cacctccagc ggcctgctcg
     2581 tgtggcgcaa ggcggacaac tggaccgcgt tcaccgatgg tgccacgacc tggatctacg
     2641 gtcccaatgg ggtggagagc cgacccaacg atgagcggtt ttcctgggaa caggatggcg
     2701 gtgccgtagt ggccggggca cctccggcag caccaccggt attc
//
LOCUS       DATALS010000261         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_208624, whole genome shotgun sequence.
ACCESSION   DATALS010000261 DATALS010000000
VERSION     DATALS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208624"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..>1805
                     /locus_tag="VHS06_05790"
     CDS             71..>1805
                     /locus_tag="VHS06_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2987666.1"
                     /translation="MPEDWIAQTQLSALAADAREALGDLREAFASASRALLQAEVSGN
                     QSMVVDAMLRLGRLSQLRGDYNQGLALLGRAVQRSQVTGTESAIIGRAAGVAAAIYLE
                     RGQRREAVRVANLAKESGLRFRDNAMQARAALATSLAMQQMGQVEIASSSMARALKLA
                     NSLPQQAPERFLVLQAHAAAMLRDGKRVQGLAETRKALHTVSSPPIKPLVEQCELMVS
                     LAGSPRRPSLSGLLRLRRLCGAMRKTDSRRWLSGGLALVAQSYARLGLRWFAASSLRR
                     ALALAQEESYIGAPLGLPLDSARLLGVAVQAGVAQEVAGTLLGVEPEAGERVLAPWLA
                     HKRPVLQTRAEQAFKGIQAGAGRAGEALLPWPGIAVDRDGASTGGKMVSPVAPVEIVA
                     LGGFRPLVGGRAEEWPSVDARDLAAYLVVNRSSRTTRDRLFRDVWPAAKAEEANVRLH
                     VALYRLREFLGDGYPAVELESEARGLYRWNGLPQLIDVERFRGLLRRVAEAGTADGHP
                     RLSDEALRRLEEAVALYQGEFLDGMGFQWCLAQREELRAMLIAATRSLIDHYMALRQW
                     DDAVRHGLKSLA"
BASE COUNT          304 a          508 c          656 g          337 t
ORIGIN      
        1 gaaggtgcgt ctgcaggagg ggcgacattc agcatcgctt gcctcggttg aggaggcggt
       61 tcgaacggca atgccggagg attggatcgc gcagacccaa ttgagcgcgt tggccgcaga
      121 tgcgcgggag gcgttggggg acctcaggga ggcatttgca tccgcatccc gcgccctgct
      181 gcaggcggag gtgagtggaa accagtccat ggtagtcgac gcgatgctgc gccttggccg
      241 gttgtcacag ctgaggggcg actacaacca gggattggcg ctgcttggca gggccgtgca
      301 gcggtctcag gtcaccggaa cggaatcggc catcatcggc agggctgccg gagttgctgc
      361 cgcaatatac ctggagcgcg ggcagcggcg agaggcagtg agggtggcca accttgccaa
      421 agagtcggga ctcaggtttc gagacaacgc aatgcaggcg cgggcggcac tggcaacatc
      481 gttggcgatg cagcagatgg ggcaggttga aatcgcatcc tcttccatgg ctcgtgccct
      541 gaagttggcg aactcgctgc cccagcaagc accggagcgc tttttagtgc ttcaggcgca
      601 tgctgcggcg atgctgcgcg acggcaaacg ggttcaggga cttgccgaga ccaggaaggc
      661 gctgcacaca gtctcatccc cgcccatcaa gccgctcgtc gagcagtgcg agctgatggt
      721 ttctcttgcg ggatcgcctc gccggccttc tctttcaggg ctccttcgac tgcggcggtt
      781 gtgtggcgcg atgcgcaaga ccgacagccg cagatggtta tccggcggcc ttgccctcgt
      841 ggcccagagt tatgctcggc tcggcctgcg ctggtttgct gcttctagtc ttcgcagggc
      901 cctggcgctt gcccaggagg agtcgtacat cggcgcaccc cttggactgc ccttggatag
      961 cgctcggctg ctgggagtgg ccgtccaggc tggcgtcgct caggaggttg ccgggactct
     1021 gctgggtgtg gagccagaag ccggcgaacg ggtgctggct ccatggctgg cacacaaacg
     1081 acccgttctg caaacgcggg ccgagcaggc atttaaggga atacaggctg gggcagggcg
     1141 tgccggggaa gccttacttc cgtggccggg catcgcggtg gaccgggatg gtgcgagtac
     1201 aggggggaag atggtgtccc ctgtcgcacc cgtcgagata gtcgcgctgg gtgggttccg
     1261 tcccctggtg ggagggcgag ccgaagagtg gccatcggtc gacgcgcggg atctcgcggc
     1321 ctacctggtc gtcaaccgct ccagtaggac gaccagggat cgtttgttcc gtgacgtctg
     1381 gcctgctgct aaagccgaag aggcaaacgt tcgcctgcac gttgcgcttt accggctcag
     1441 ggagtttctg ggggacggtt atccggcggt ggagttggaa tcggaggcaa gggggctcta
     1501 ccgatggaat gggttgccac aactcatcga cgtcgagcgc ttccgagggc tgctgagacg
     1561 ggtggccgag gcgggtacgg cagacggtca cccgcggctg agcgacgaag cgttgcggcg
     1621 cctggaggag gccgtagcgc tctaccaggg cgagttcctc gacggcatgg ggtttcagtg
     1681 gtgtctggcg cagagggagg aactccgtgc catgttgatt gccgcgaccc ggtcgctcat
     1741 agaccactac atggcactca ggcagtggga tgatgccgtt cgacatggtc tcaagtcgct
     1801 cgcca
//
LOCUS       DATALS010000262         3619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_57318,
            whole genome shotgun sequence.
ACCESSION   DATALS010000262 DATALS010000000
VERSION     DATALS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57318"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /gene="rpsE"
                     /locus_tag="VHS06_05795"
     CDS             <1..306
                     /gene="rpsE"
                     /locus_tag="VHS06_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544716.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX2987667.1"
                     /translation="VRVPLRANTIPHEVLAQFGAAKVMLKPASPGTGVIAGGGVRAVV
                     EAAGIKDILTKSLGSSNAINVVQAAMIGLKGLKDPETVAQQRGKKLADLVGRAATSG"
     gene            299..487
                     /gene="rpmD"
                     /locus_tag="VHS06_05800"
     CDS             299..487
                     /gene="rpmD"
                     /locus_tag="VHS06_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX2987668.1"
                     /translation="MAKKLVITWTKSAIGYSHRQKDTIRSLGLHRLNETVEKPDTSEF
                     RGMVDRVRHLVKVEEEEE"
     gene            484..933
                     /gene="rplO"
                     /locus_tag="VHS06_05805"
     CDS             484..933
                     /gene="rplO"
                     /locus_tag="VHS06_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX2987669.1"
                     /translation="MKLHELAPTEGAKKARKRVGRGNGSGHGTFSGRGSKGQKARSGG
                     QVNPRFEGGQIPIVLRLPRKRGFNNIFKQRFHIVNLDSLDQFAAKTEVTPELLAEAGL
                     IRDTFLPVKLLGTGELAKPMTIRVHRWSQSAQQKVEAAGGALEKVQG"
     gene            930..2216
                     /gene="secY"
                     /locus_tag="VHS06_05810"
     CDS             930..2216
                     /gene="secY"
                     /locus_tag="VHS06_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX2987670.1"
                     /translation="MIDSLVNAFRIPDLRRKLLYTFGMLVIFRFISHVPVPGVNAEAL
                     RRLFESNQLIGMLDLFSGGAMSNFSVAAMGVYPYITATIIMQLLIPMVPALQDLSKEG
                     ESGRNKLNQYMHWLTVPLAFLQALGTGSLLASSQLGVIPNFNLVSNTIPTLAIIMTMT
                     AGTVLLVWIGELITENGIGNGVSIIIFAGIVAKLPSSVVSTLVAGTNYLGVIVFAILG
                     LLTVAAIVVVQEGQRRIPVQFAKRIRGNRMYGGSATHIPMRVNSAGMIPLIFAMSIML
                     FPGTVASYFVYTGGFVGDVANFFANVFNPAHIVYWVLYFFMVVAFTFFYTMVIFQQQN
                     IPESLQKNGGFVPGIRPGRPTGEYLNKVLIRITWFGAIFLGVIAILPFFVQNLTSVQA
                     LTLSSTALLIVVGVVLDTMKQLESQLLMRNYQGFIK"
     gene            2230..2898
                     /locus_tag="VHS06_05815"
     CDS             2230..2898
                     /locus_tag="VHS06_05815"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX2987671.1"
                     /translation="MYVVFLGAPGAGKGTQAGVLGEKMGVLHLATGDVLRAAVKQETP
                     LGLEAKGYMERGELVPDSLVVNLVVDRVAEGDAANGAILDGFPRNLSQARALDEALKR
                     RGAKVDLVLYLKVREEALVARLAGRWECGTCRTPYHEQTKPPKAAGVCDQCGGALTQR
                     PDDVPETVRRRLQVYFEQTAPLLDYYRAQDVLREINGERPIAEVTAQIQSVVASVEGK
                     SRGA"
     gene            2900..3118
                     /gene="infA"
                     /locus_tag="VHS06_05820"
     CDS             2900..3118
                     /gene="infA"
                     /locus_tag="VHS06_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX2987672.1"
                     /translation="MAKSDAVEVEGTVVEALPNGMFRIEIPAGHKVLAHVSGKIRLNF
                     IKILPGDKVKVELSPYDVSRGRIVYRSR"
     gene            3138..3251
                     /gene="rpmJ"
                     /locus_tag="VHS06_05825"
     CDS             3138..3251
                     /gene="rpmJ"
                     /locus_tag="VHS06_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX2987673.1"
                     /translation="MKVSASVKPRCEKCKVIRRRGVVMVICKNPRHKQRQG"
     gene            3389..>3619
                     /gene="rpsM"
                     /locus_tag="VHS06_05830"
     CDS             3389..>3619
                     /gene="rpsM"
                     /locus_tag="VHS06_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX2987674.1"
                     /translation="MARIAGVDIPRDKRVEVALTYIYGIGPTTSQKILARTRISPDTR
                     GCLLEIHLEPAPYRLGHIIRPLGEGSSALVANA"
BASE COUNT          731 a          942 c         1208 g          738 t
ORIGIN      
        1 gtgcgtgtgc ctctgcgagc caataccatc ccgcacgagg tgctggcgca gtttggcgcg
       61 gcaaaggtga tgctcaagcc tgcgtcccct ggtaccggcg ttatcgctgg cggcggtgtg
      121 cgagccgtcg tcgaagctgc cggcatcaag gacatcctca ccaagtcgct ggggagctcc
      181 aacgccatca acgtggtcca ggctgccatg atcggtctga agggacttaa ggatcccgag
      241 accgttgcac agcagcgggg caagaagctt gccgacctgg tagggagggc tgctaccagt
      301 ggctaagaag ctagtaatca cctggacgaa gagtgccatc gggtactccc accggcagaa
      361 ggacacaatc cggtccctcg gtctgcatag gctgaacgaa acggtggaga agcccgatac
      421 gtcggagttc cggggcatgg ttgatagggt gagacacctg gtcaaggtgg aagaagagga
      481 agaatgaaac tgcacgaact ggcgccaacc gagggtgcca agaaggctag aaagagagtg
      541 ggccgcggga acgggtccgg ccacggcacc ttttccggtc gcggctccaa ggggcagaaa
      601 gcgcgatccg gcggccaggt gaacccccgg ttcgagggtg gccagatccc catcgtgctg
      661 cggttgcctc gtaagagggg cttcaacaat attttcaagc agcgcttcca catcgtgaac
      721 ctggacagcc tggatcagtt tgcggcgaag actgaggtca ctcctgagtt gctggccgag
      781 gccggcctca tccgcgacac ttttcttccc gtaaagctgt tgggcacagg ggagttggcg
      841 aagccgatga cgatccgggt ccacaggtgg tcccagagcg ctcagcagaa agtcgaagcg
      901 gcgggcggcg cccttgaaaa ggtccaagga tgatcgattc cctagtcaac gcattcagga
      961 taccggatct tcggcgcaag cttctgtaca ccttcggaat gctggttata ttccgattta
     1021 tctcgcatgt gccggttccc ggggtgaatg cagaggcgtt gcgtaggctg ttcgagagca
     1081 accagttgat tggaatgctg gacctgttct cgggcggtgc aatgtctaac ttctcggtag
     1141 ctgcaatggg cgtctacccg tacatcacgg cgacgatcat catgcagctg ctgataccga
     1201 tggtgccggc gctgcaggac ctctccaagg agggggagtc gggcaggaac aagctcaacc
     1261 agtacatgca ctggttgacg gttccactgg cgttcctgca ggctctgggc acggggtcgc
     1321 tgcttgccag tagccagttg ggggttatcc ccaacttcaa cttggtttcc aacaccatcc
     1381 ccacgctggc gatcatcatg acgatgacag ccggtacggt gctgctggtg tggataggcg
     1441 agttgattac ggagaacggg atcgggaacg gggtctcgat catcatcttc gccggtatag
     1501 tagcgaagtt gcccagttcg gtggtctcga ctctggtggc cggcaccaac tacctgggcg
     1561 tgatcgtgtt tgcgatcctc ggcctgctta ctgtggccgc catcgtggtg gtgcaggagg
     1621 ggcagaggcg gatccctgtg cagtttgcca agcggatccg tggcaacagg atgtacggcg
     1681 gcagtgccac ccacattccg atgagggtga attcggccgg tatgatcccg ctgattttcg
     1741 cgatgtcgat catgctgttc ccaggtacgg ttgccagcta cttcgtatac acaggtgggt
     1801 tcgtggggga tgtggcgaac ttcttcgcca acgtcttcaa tcctgcgcat atcgtgtact
     1861 gggtgctgta tttcttcatg gtggtggcgt ttaccttctt ctacaccatg gtgatcttcc
     1921 agcagcagaa catccctgag agtttgcaga agaacggcgg gtttgttccg ggcatcagac
     1981 ctggtcggcc caccggtgag tatctgaaca aggtactgat acggataacc tggttcggtg
     2041 cgatattcct gggcgtgatc gcgatcctgc cattcttcgt gcagaacctg accagtgttc
     2101 aggctctcac cctgagtagc acggcgctgc tgattgtagt cggtgtggtg ctggacacga
     2161 tgaagcagct ggagtcgcag ctacttatgc ggaactacca ggggttcatc aagtaggagc
     2221 ggtttgacga tgtacgtggt gtttctcggg gctcccggtg ccggcaaggg cacccaggcg
     2281 ggggtgctgg gcgagaagat gggtgtgctt cacctggcaa ccggcgacgt gttgcgagca
     2341 gcggtgaagc aggagacccc gcttggcctg gaagccaagg gatacatgga acgcggagag
     2401 cttgtgccgg actcgctcgt ggtcaacctg gtggtcgatc gagtggcgga aggcgatgcg
     2461 gcgaatggag cgatcctgga cggttttcca aggaacctga gccaggctcg ggccttggat
     2521 gaggcgctca agaggcgcgg ggcgaaggtg gacctggttc tctacctgaa ggtgcgtgaa
     2581 gaagcgttgg ttgccaggct tgccgggcgg tgggagtgcg gtacctgccg aactccttac
     2641 cacgagcaga cgaagccccc caaggcggcg ggcgtttgcg accagtgcgg aggagccctc
     2701 acccagcggc ctgatgatgt gcccgagacg gtacggcgca ggctccaggt gtatttcgag
     2761 caaacagccc cgcttctgga ctactaccgg gcgcaggatg tcctgcggga gatcaacggt
     2821 gagcggccga tagcggaagt caccgctcag attcagtccg tggtggcgtc tgtggaaggc
     2881 aagtctcggg gagcgtagag tggccaaatc cgatgccgtt gaggttgagg ggactgtggt
     2941 tgaggctctg ccgaatggaa tgttcaggat agagatccca gccggccaca aggtgcttgc
     3001 tcacgtgtcc gggaagatca ggctgaattt cattaagata cttcctggag acaaggtcaa
     3061 ggtggagcta tcaccgtacg atgtctctcg cggacggatc gtgtacaggt cgagatagat
     3121 acgtggagta gggaccaatg aaggtatcgg catcggtaaa gccgcgctgc gaaaagtgca
     3181 aggtcattag gcggcgaggc gttgttatgg taatatgtaa gaatccccgt cacaagcagc
     3241 ggcaggggta agaagctgct aggggtgtat ggcggccgcg agcgcgaccc acttgagggg
     3301 tttgcgcgag ttgcgccgat ccttactgcg gcaagaactc ggttctcctg gttcagggga
     3361 cggtttgaaa tcgggaaagg aggcggtagt ggcccgcatc gcaggggttg atataccgcg
     3421 ggacaaaaga gttgaggtcg ctctgaccta catttacggt atcggtccga cgaccagcca
     3481 gaagatcctg gctcgcacca ggatcagtcc ggacacgcgg ggctgtttgc tcgaaataca
     3541 cctggagcct gcgccgtacc gtctcgggca catcatcagg ccgctgggtg agggctcctc
     3601 cgcactggtc gcaaacgcc
//
LOCUS       DATALS010000263         3512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_26960,
            whole genome shotgun sequence.
ACCESSION   DATALS010000263 DATALS010000000
VERSION     DATALS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26960"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VHS06_05835"
     CDS             complement(<1..347)
                     /locus_tag="VHS06_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987675.1"
                     /translation="MPNPTLNRRYQKLIDQLDHVHPDTREMFLYLFAMVAAEQGIISV
                     ERTQDERGRQVVVMLDPKKKQEFKVAYPEGLDPTFARVAVAEMERLLSDDSVGTSMDS
                     TLFRQLYENHPCR"
     gene            complement(378..1004)
                     /locus_tag="VHS06_05840"
     CDS             complement(378..1004)
                     /locus_tag="VHS06_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2987676.1"
                     /translation="MEIVWLGHSCFRLKGRDTTVITDPFDRSSGYSLGNVSADLVTVS
                     HTAPDHSNVAAVDGNPQVVDGPGEYEVRGVLITGVATRRDGEKGRNTAYLMEIDDLTV
                     CHLGDLGHILTPPQVEQMNSADILLIPVGGNNTINASQAAEVISQIEPRIVIPMHFKT
                     EATTLELDPISKFQREMGLQELKPQPRLVVSKGSLPEETTVVLLDYKR"
     gene            complement(1319..1711)
                     /locus_tag="VHS06_05845"
     CDS             complement(1319..1711)
                     /locus_tag="VHS06_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987677.1"
                     /translation="MQPKKLLLIDDEPAIQTLVRLVMEGDGWQVLVAGEGTPGLALAR
                     EQHPDVILLDVALPDISGLEVCRQLKSDPGTSSIPVAMLTAMAQETDRRAAMALGADD
                     YVTKPWRPAALIARISGLMEKSAGAKEN"
     gene            complement(1838..>3512)
                     /locus_tag="VHS06_05850"
     CDS             complement(1838..>3512)
                     /locus_tag="VHS06_05850"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX2987678.1"
                     /translation="HQEFLLLLLLFVTLRLMLLVISRPQGYFYDASDYDFYLEFGKLA
                     DRGLYPMLHYWTEYPPVFPWAAVAAYRAVEAIPNLNGSPIFWFRIVLGSGLLLFDVGN
                     LILIYWLGRAIFTPARAIQIGWSYALLFAPLHVWLGWFDTSPLFFLLLSVALTVSGRH
                     QLAAVASGFGFMVKVFPILTMPLIMKSERRPAGRAKLLAACALIVGAIAAPFMVMAPA
                     YLLASFKSMVSRSPWETPWAILEHYYGYGQVAPLQDRTDPASASFVAYPSHLPWPLIS
                     LTFALIFILIWTRPFDARRPLNAVTFTGLSVNLFLLYSRGYSPQFLVYLVPFALLSLP
                     AFRSVAYISVLSAINLIEYPAYLTLFRGQNWLLTDLVAMRTAILLLLCWEYLATLQLL
                     PSFQLARRTAAAVTAGIFVLWAILSYPAAGQAWEQNSLTQHPDAALVAYLQANAGQGS
                     VVVFTDQYLYRELYPYLYESTHLVLMDPATEQQVVSNSGVGSPAKEGGPTMVEQLALL
                     STQYREIYGVRQMDDLNGNRLEQLLAFVGRLEAGRRINDLSVSRWAIGR"
BASE COUNT          738 a         1018 c         1067 g          689 t
ORIGIN      
        1 ctgcggcagg ggtggttctc gtacagttgg cggaagaggg tgctatccat ggatgttccg
       61 accgagtcgt cactcaggag acgttccatc tccgccaccg cgacacgggc gaaggtgggg
      121 tccaacccct ctgggtaggc gaccttgaac tcctgctttt tctttggatc cagcataacc
      181 acaacctgcc gtccccgttc atcctgggtc ctctccacgc ttatgatccc ctgctccgcg
      241 gccaccatgg cgaagaggta gaggaacatc tccctggtgt cagggtgtac gtggtccagc
      301 tggtcgatca gcttctggta tcggcggttc agggtcgggt ttggcatcga tggatccttc
      361 cagtagtgcg cagtgctcta gcgcttgtag tcgaggagga cgacggttgt ctcctcgggg
      421 aggctgcctt tgctaactac cagcctcggc tgcggcttca attcctgaag gcccatctct
      481 cgctgaaact tgctgatggg gtccagttca agggtcgtag cctcagtctt gaagtgcatg
      541 gggattacga tccgcggctc gatttggctg atcacctctg cggcctggct ggcgttgatg
      601 gtgttgttgc caccgaccgg gatcaacaga atatccgcgc tgttcatctg ctccacctga
      661 gggggagtca agatgtgccc caggtctccg agatggcata ccgtgagatc gtcgatctcc
      721 atgaggtacg cggtgtttcg tcccttctcc ccgtctctac gggtggcaac ccccgtgatc
      781 aacacgcctc tcacctcgta ctccccaggc ccatccacga cctgtgggtt accatcaacc
      841 gcagcaacgt tgctgtgatc aggggcagtg tgggagacgg tgaccaggtc tgccgaaaca
      901 ttgccgaggc tatagcctga gcttcggtcg aaggggtcgg tgattactgt tgtgtcgcgg
      961 cccttcagcc tgaagcagct gtggccgagc caaacgattt ccaatgcggc actcctgtcg
     1021 aaaacgagct gtcgcccacc tgtccggatc tgccggacga taccctcgcg agaattcgga
     1081 cgccccagcc gttcacgctc ggtgtgacgg ccgtgccgaa agagacgaaa gcgccgtatc
     1141 cgaaaaaccg ccgagacccg agagctagaa aagagagggg gtgggcggag gtctcttgag
     1201 aatcaaccct gtttagattc tatctccgcc ccaatgcagt gtcaacaata gaagcatatg
     1261 gggacgtaaa gcaagcgaaa gccagtcggt ctcaggaaga caaatcagct cgggctggtc
     1321 agttctcttt ggcgccggcg ctcttctcca ttaggccgga aatcctggcg atcagcgccg
     1381 ccggcctcca cggcttcgta acgtagtcgt cggctcccag tgccatggct gcccgtcggt
     1441 cggtctcctg agccatcgcc gtgagcatgg cgaccgggat cgagctggta cccgggtcgg
     1501 acttcaactg ccggcagact tcgagcccag atatatcagg gagcgcgaca tccaaaagaa
     1561 ttacgtcggg gtgttgctct cgcgccaggg ctagtccggg tgttccttca cctgccacca
     1621 gcacctgcca gccgtcgccc tccatgacca ggcgaaccag cgtctggatg gctggctcat
     1681 cgtcaatgag cagcagcttc ttaggttgca accacctacc tccaggccga tagtgctagt
     1741 cctgagcgaa ttgccgtttg ggaaacccgc aggggagaga agcgtttgca gtatagtagc
     1801 tacgatgact ggtggcaata tgtcgacaaa agacatccta tctgccgatc gcccatcgac
     1861 tcaccgacag gtcattgatc cttctgccgg cctccaggcg gccaacgaag gcaagtagtt
     1921 gctccaggcg gttgccgttc aggtcgtcca tctgcctgac tccgtagatc tctctgtact
     1981 gagttgagag aagggcgagt tgctccacca tagtggggcc cccctccttt gctggggaac
     2041 ctaccccact attggacacg acctgttgct ccgtcgccgg atccatcagc accaggtggg
     2101 tgctctcgta caggtagggg tatagctccc tgtagaggta ctgatcggtg aagaccacca
     2161 cggaaccttg ccctgcattg gcttgcaggt aggccaccag cgcggcatcg gggtgttggg
     2221 taagcgaatt ctgctcccat gcctgaccgg ctgcggggta actgagtatt gcccatagaa
     2281 cgaatattcc tgccgtgact gctgccgcag tccttctcgc gagctgaaac gatggaagca
     2341 gctgcagggt tgcaaggtat tcccagcaga gcaacagcag gatggcggtg cgcatcgcaa
     2401 cgaggtcggt cagcagccag ttctgccccc tgaacagcgt gaggtatgcg gggtactcga
     2461 tcaggttgat ggcgctgagc acggagatgt atgccacaga ccggaaggcg ggcagcgaca
     2521 agagggcaaa tggcaccagg tagaccagga actgcggact gtagcccctc gagtagagca
     2581 ggaacagatt cactgaaagt cccgtgaacg tcacggcatt tagcggcctg cgggcatcga
     2641 atggccgtgt ccagatgagg atgaagatca aggcaaaggt caggctgata agtggccacg
     2701 gcaagtggct tggatacgcc acaaaggaag ccgaggccgg atctgttcta tcctgcagtg
     2761 gcgcaacctg accgtagccg taatagtgct ccaggatagc ccagggagtc tcccaggggg
     2821 atcgggagac catggacttg aacgatgcca gcaggtaggc aggcgccatg accatgaagg
     2881 gagcagcgat tgcccccacg atcaaagcgc aagctgccag caacttcgcc cgccctgccg
     2941 gccggcgctc cgacttcatg atgagcggca tggtgaggat tgggaacacc ttgaccatga
     3001 agccgaagcc cgatgcgacg gccgccagtt gatgccttcc gctcacggtc aacgccactg
     3061 aaaggagtag gaagaagagc ggggacgtat cgaaccaccc cagccacacg tgcagcggcg
     3121 cgaaaaggag cgcgtagctc caaccgatct ggatcgctct ggctggagtg aagatcgcgc
     3181 gccccagcca gtagatgagg atgagattcc ccacatcgaa tagcaggagc ccgcttccga
     3241 ggactattct gaaccagaag atggggctcc cgttgagatt ggggatcgcc tccacggccc
     3301 tgtaggctgc tacggctgcc cacgggaaca ccggcgggta ttctgtccag tagtggagca
     3361 tgggatacag cccgcgatcg gccagcttcc cgaactccag gtagaaatcg tagtcagacg
     3421 catcgtagaa gtacccctgc ggacgtgaga tcaccagcag catcaaccgc agggtgacga
     3481 agagcagcag gaggagcagg aactcctgat gg
//
LOCUS       DATALS010000264         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_383754, whole genome shotgun sequence.
ACCESSION   DATALS010000264 DATALS010000000
VERSION     DATALS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383754"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..2327)
                     /locus_tag="VHS06_05855"
     CDS             complement(204..2327)
                     /locus_tag="VHS06_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX2987679.1"
                     /translation="MRFTVQKKILSGFFVVLVLMGIIGWIGLNKTNTFAQQLNDMYSD
                     SVVPVSYAGELESLVMTRGRDLRNVIIGALTKDEKALSAGVAAMADDDRQISDILKKY
                     DALSMSQEQKDSLASYKTAYSDYKTISDSIAKAASEGKVDTALAQQNSAATSAAKVWD
                     AAQKLTKYSVDGAAKESQEASQAAGQATVSTVAILAVAVLLGLGIAAYIGRDIAGVVG
                     LMSIVVNRLTVGDLNRDMDERTKDRMRNRRDELGDIGKGLGKTMIYIRSMAGHAQSIA
                     NGDLTEEIHPNSEKDELGVAFRDMTAGLREMIGQVASSSTALAEASHQLSSASTQAGA
                     ATQQIATTIQQVASGNQETSASVQETTASVEQLARAIDQIARGAEDQSQSIQKVSSSV
                     AQLGGSITQVASLSREVSSSTEQSRTAATAGGESIKKTIRGMASIKESTGTVGVKIQD
                     LQGYSEQIGAIVEAIDDIAEQTNLLALNAAIEAARAGEHGRGFAVVADEVRKLAERSG
                     QETKQIAGLIAQVQKGTREAVEASGQGHDAVEQGLKLAEEAGDALKDILSGAEAVATQ
                     VGQISVAVQQMESASQEVVGLMDSVSAVVEESTAATEEMAASSQQVNGAIEKVAAVSE
                     ETSASAEEVSASTEEMRSQVEEMVAQSEELSRMAEELQAAVSRFKVGEQAEVVMKRRQ
                     ADWQRQPQVLRQPTRQNDVKPLPIG"
     gene            complement(2505..>2939)
                     /locus_tag="VHS06_05860"
     CDS             complement(2505..>2939)
                     /locus_tag="VHS06_05860"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HEX2987680.1"
                     /translation="PLDADIAPRPRATTLRSTSASSATATVFSDGACQGNPGPGGYGV
                     IVKLPGQPPRELSGGKAHTTNNEMEMTAAIEGLKAAVAGGAKEITVISDSEYLVKGMT
                     GWIKGWLRNGWKTSSGSRPGTAAYYPHSFRADILAERNATTF"
BASE COUNT          516 a          977 c          779 g          667 t
ORIGIN      
        1 cggatagagg cttcaatggg aagctatggt cgcgctccat cctgctcgtt ctagaacaat
       61 gcgacgctca ggtcaaccac gcgtaccacc cggtcaccgc gatcgcccat acccctcttc
      121 gagtcgaagc gactccgaca aaaggggatc ggcgggaaaa tcgccccgcc gatcccctgt
      181 catgtaggag gcttgaggat cgtctaccct atcgggagcg gcttgacatc gttttgcctg
      241 gtcggttgcc tcagaacctg cggctgtcgc tgccagtcgg cttgccgccg tttcatcacc
      301 acttcggcct gctcccccac cttgaaccgg ctcaccgcag cctgaagctc ctcggccatt
      361 cgtgagagct cctccgactg ggccaccatc tcctccacct gcgacctcat ctcctcggtg
      421 gaggcgctca cctcttccgc cgaggcgctc gtctcctcac tcaccgccgc caccttctcg
      481 attgcgccgt tgacctgctg ggatgaggca gccatctcct cggttgccgc cgtcgactcc
      541 tccaccaccg cggagaccga gtccatcaga cccactactt cctgagaggc cgactccatc
      601 tgctgcacgg cgacggatat ctggcccact tgagtggcaa cggcctccgc tcccgagagg
      661 atgtccttga gggcatcccc tgcttcctcg gccagcttca gcccctgctc caccgcgtcg
      721 tggccctggc ccgaggcctc caccgcctcg cgggtcccct tctgaacctg cgcaatcaga
      781 cccgcaatct gcttggtctc ctgcccgctc ctctcggcca gcttcctgac ctcatcagcc
      841 acaactgcga agcctcgtcc gtgctcgccc gctctggcag cctcgatcgc agcattcagt
      901 gccaacaggt tcgtctgctc cgcgatgtcg tctatcgcct ccacgatggc gccgatctgc
      961 tccgagtagc cctggagatc ctggatcttc acccctactg tgccggtgct ctccttgatg
     1021 ctcgccattc cccggatggt cttcttgatc gactctcctc cggcggtggc tgcagtccgg
     1081 gactgctcgg tagaagaact gacctccctg gatagagacg ccacctgggt aatcgagcca
     1141 cccaactggg ccaccgagga ggaaaccttc tgaatggact gcgactgatc ctcggctccg
     1201 cgggcaattt gatcgatggc gcgggcgagc tgctctacag atgcagtggt ttcctgaacc
     1261 gaggcactcg tttcctggtt gccggaggcc acctgttgga tagtggtggc gatttgctga
     1321 gtcgcggcac cggcctgggt ggatgccgat gagagctgat gtgatgcttc agccaaggcc
     1381 gtcgacgagg aagccacctg gccgatcatc tctcgcagac cggcggtcat gtctcggaaa
     1441 gccaccccga gctcgtcctt ctccgaattg ggatggatct cttctgtcag atccccattc
     1501 gcgatgctct gggcgtgacc tgccatggag cggatgtata tcatagtctt accaaggcct
     1561 tttccaatgt cacccaattc gtccctacgg tttcgcatcc ggtccttggt tcgttcgtcc
     1621 atgtcgcggt tgaggtcgcc aaccgtgagc cggttgacca caatcgacat aagccccact
     1681 acaccagcga tgtctcgccc gatatacgcc gcaattccca gccccaacag aacagccaca
     1741 gccaggatcg caactgtgga tacggttgcc tgtcccgccg cctggctggc ctcctggctc
     1801 tccttcgcgg cgccatcgac gctgtatttc gtgagcttct gcgccgcatc ccagactttg
     1861 gccgcactgg tagcagcact gttctgttgg gctagtgctg tatctacctt tccctcggag
     1921 gccgccttgg caatgctatc gctgatggtc ttgtaatctg agtaggcagt cttgtaggaa
     1981 gccagactgt ccttctgctc ctgggacatc gagagagcat cgtacttttt caggatatcg
     2041 gagatttgcc tatcgtcgtc ggccatggca gccacaccag ccgacagcgc cttctcgtct
     2101 ttagtgagtg caccgatgat tacgttccga aggtcccgcc ccctggtcat cacaaggctt
     2161 tccaactcac ccgcatagga cacgggcacc accgagtcgg agtacatgtc gttcaactgt
     2221 tgggcaaatg tattggtttt gttcagccct atccatccga taatccccat caacaccaga
     2281 accacgaaga aaccagacag tatcttcttc tgaaccgtga atcgcactgc gctctctcct
     2341 tctcatagta ctgttggctg ggtgcaaacc gttctattaa ttaacgatcg gcaggctttg
     2401 tcatctatcg tagaagcaat cggcatatgt aaagcttaat aattgttatc cttgacaagg
     2461 aaggcatcta agttggcctc catagacagg ctacagttta gctactaaaa agtggtagca
     2521 ttgcgctcgg cgagaatgtc agcccgaaag gagtgagggt aatatgcagc ggttccaggt
     2581 cttgaccccg aactggtttt ccagccgttg cgcagccatc ccttgatcca gccggtcatc
     2641 cccttcacca ggtactcact gtcggagatc accgtgatct ccttcgcccc gccggccacg
     2701 gccgctttca gtccctctat ggcggcagtc atctccatct cgttgttggt cgtgtgggcc
     2761 ttgccgcccg atagctccct gggcggttgc cccggcagct tcactatcac cccgtagcct
     2821 cccggccccg ggttcccctg gcaggcgccg tcggagaaga ccgtggccgt ggcggacgat
     2881 gcggaggtgg acctgagcgt cgtcgcccgt gggcggggtg cgatgtcggc atccagcgg
//
LOCUS       DATALS010000265         5616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_383803, whole genome shotgun sequence.
ACCESSION   DATALS010000265 DATALS010000000
VERSION     DATALS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383803"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VHS06_05865"
     CDS             complement(<1..562)
                     /locus_tag="VHS06_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEX2987681.1"
                     /translation="MAWGTQGVDFEVRVDYDKLRKERLSKARAQMERAGLGAILCLDP
                     NNVRYLTSTHLGEWSRDKFTRYCVLPRGGDPVLFDFGSAVKVKKQHNPWIADHVRPAV
                     GWMRGAVPQLESSSDRFMEEIVGILSEYGVQKEPLGIDLADATLLEALRKSRLEVADG
                     QDAMLEARLRKSPDEIQLLKAAAAMAD"
     gene            complement(577..1464)
                     /locus_tag="VHS06_05870"
     CDS             complement(577..1464)
                     /locus_tag="VHS06_05870"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2987682.1"
                     /translation="MGRVGFVGMGNMGSRMAARLVQAGHEVAVYNRTRERAVPLAAIG
                     ARIAGSPAEACAEADIAFTNVADGTALKAVCLGSSGVLSAGPAPKILVDMSTVGPVES
                     AEVAAVADPLGVAYLRSPVSGSTVLAEAGKLTIFASGNKEAYDAAEPYLAAIGETRFY
                     VGPGDNARYLKLVINMMVATQVEILSEAVVLGEKAGLDWAEMIEVISNSVVASPLVKY
                     KAAPLRARNFAPAFALGLMIKDLDLAIAAAQKKGVELPVTERVREFYSQAAEAGLADK
                     DFLAATLLLEKRAAIAPTA"
     gene            complement(2011..2337)
                     /locus_tag="VHS06_05875"
     CDS             complement(2011..2337)
                     /locus_tag="VHS06_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987683.1"
                     /translation="MKVCALIGKSTCPWFRPPSGAYDDRVVQLAGQQGYYTIYWTADS
                     ADWRPGIDAAAVKERLLRYARPGAILVQHLTSVQSAEVLPEVLRTLKERGYELVTVSE
                     FLATAD"
     gene            complement(2334..2762)
                     /locus_tag="VHS06_05880"
     CDS             complement(2334..2762)
                     /locus_tag="VHS06_05880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX2987684.1"
                     /translation="MSRLLLPAVVFLLLTLCPQVTAAFDPAQTQPRLSQLSETTPTPS
                     ADWQPLPPSLVVQTDSNKVALTFNAASYPPFQPAILDALREAGVRCTVFLTGDFVDSY
                     PELVVQMAREGNEIGNHGDTHADMPGQTDEEMVAELGGWT"
     gene            complement(2870..3697)
                     /locus_tag="VHS06_05885"
     CDS             complement(2870..3697)
                     /locus_tag="VHS06_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX2987685.1"
                     /translation="MSYVEEMGYPGIDLDLEGINAEDRDAFSDLVARAADALHKRGKM
                     LTLAIPAKGSDVRTGWAGPYDYAALGKSADMILLMTYDYSWPGGPPGSIAPQTWVDKV
                     AAYAVSQMPAEKVLIGLAFYGYDWNKTLGGRAKSLLYPQAAALAKQHETEIATDDASR
                     SGTFSYSAKAGDGVPPPPAVPAVQHDIAVRAPAQCKVQPAAPTPQPPATGPAPTPRPA
                     PPTPAVQEHEVWLEDATSVAGKLQIATCYSLGGVGAWRLGQEDPSVWPELAGYRSGK"
     gene            3790..5217
                     /locus_tag="VHS06_05890"
     CDS             3790..5217
                     /locus_tag="VHS06_05890"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2987686.1"
                     /translation="MDVHSHTDSTIQVNPTAESTIRQGTTTEIVGNCGSSMAPLTDAS
                     RDRTTARLRKYGYDGPIDWATFGQYLESVQRMGTSINLAHLVGHNALRGAAEVSGPNA
                     TEEQLRAMEGMVQEAMEAGALGISTGLEFEPGRQAPTTEVIRLARVVGRYDGHYVSHI
                     RNRDARVQEAVEEFLEIQRAGGTRGQISHLNTRHNTGVPEGGWQRAADTMEQARRDGL
                     NVLADMTPFAFGTGSMASILPPWVRDGGPTRTAELLRDPSVRTRLRTECDRYWRFIHR
                     GEWDRVRLLSSAEYPELCSKTFVEISQLWDKDPWDCYFDILAAAGPKLDALSLVAILF
                     TEGHLKETISHPLFCLGVDGVSSRIDGPLAEQTRTPINFCGMTHYLTYHVREKHTLSL
                     EEAIRKMTSMTACHFGLRGRGLLQADCFADVVVFDFDRLEDVSTLEQPVAYVRGVEHV
                     LVNGVPIIDGGNHTGARPGRNLLRG"
     gene            complement(5349..>5616)
                     /locus_tag="VHS06_05895"
     CDS             complement(5349..>5616)
                     /locus_tag="VHS06_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987687.1"
                     /translation="AVRAPAQCKVQPAAPTPQPPATGPAPTPRPAPPTPDVQEHEVWL
                     EDATSVAGKLQIATRYGLGGVGAWRLGQEDPGVWPELAGYRSGK"
BASE COUNT         1049 a         1820 c         1740 g         1007 t
ORIGIN      
        1 cgtctgccat tgccgcggcc gccttcagca gctggatctc atcgggagac ttacgaagcc
       61 tcgcctccag catcgcgtct tgcccatccg cgacctctag ccgggatttc cgcaaggcct
      121 cgagaagagt ggcatcggcg aggtcgatgc cgaggggttc cttctggacg ccgtactcac
      181 ttagaattcc aacgatctcc tccatgaatc ggtcggagga ggactccaac tgaggcaccg
      241 cacccctcat ccatccgaca gcgggacgaa cgtggtcggc aatccatgga ttgtgctgct
      301 tcttgacctt tacggcgctg ccgaagtcga acagaaccgg atctccgccc cttgggagaa
      361 cacagtagcg agtaaacttg tcgcggctcc actcaccgag atgggttgag gtcaggtagc
      421 gaacattgtt gggatccagg cacaagatgg cgcccaaccc ggccctctcc atctgcgccc
      481 gagctttgct cagccgctcc ttcctcaact tgtcgtagtc aacgcgcact tcgaagtcga
      541 cgccctgagt accccatgcc atggtgcctt acctccttac gcagtaggtg cgatggcagc
      601 cctcttctcg agcagtaggg tggcagccag gaagtccttg tcagccaggc ctgcttcggc
      661 cgcctggctg taaaactccc gaactcgctc cgtaaccggc agttcaaccc ctttcttctg
      721 tgcagcggca atggccaggt caagatcttt gatcatcagc cctagcgcaa atgccggagc
      781 aaagtttcgc gcacgcagag gagctgcctt gtacttgacc aggggtgacg ccactacact
      841 gttgctgatt acctctatca tctcggccca gtcgagtccg gccttctctc cgagcacaac
      901 cgcttcggac aggatctcaa cttgagtcgc gaccatcatg ttgataacca gcttcaagta
      961 gcgagcgttg tctccggggc cgacgtagaa gcgggtttcg ccgatggccg ccaggtatgg
     1021 ctccgcagcg tcgtatgctt ccttgttgcc cgaggcgaag atcgtcagct tgccagcctc
     1081 ggcgagtacc gtgctgccgc taacggggga tctaagatac gcgacaccca ggggatcagc
     1141 gactgcggcg acctcagccg actccacagg accaaccgtg ctcatgtcca caagaatctt
     1201 gggggctggt ccggctgaca gaacaccact agaaccgagg catactgcct tcagcgcagt
     1261 cccatctgcg acgttggtga aggcgatgtc cgcttcggca catgcctctg ccgggctgcc
     1321 ggcaatcctt gcacctatcg ctgcaagggg cacggcccgt tcacgcgtac gattgtagac
     1381 agcgacctca tggcctgcct ggacgagccg ggctgccatc ctcgagccca tgttccccat
     1441 tccgacgaag cctacacgac ccattgccat ccctcctcac ctttactatc cccctgatgg
     1501 accgcaagtg gatgaagata tcgacatgtg ttaatcgcca ctgcactggg cacaacttta
     1561 tggagggaac taacacagcc tcttctgaac aaatcctggg agggggtcca acccaaaagc
     1621 tcaagcctcg acgcgctcag agagctgccc tctagggtgc aaggtgcggc gaggacgcga
     1681 atcgagcatc tgcaaccaga accagagccg tggggtgaga gggattcgca gggcaccttt
     1741 gcctgagcac gtcagcatcc ccatcaacat ggtagcgcga ccagacaaag gtgtcaagtg
     1801 ccactttcca ttcaatgcag tttcactgca cacttgggct cagattgagt cgagatgtgg
     1861 gttcgggggc gtatggttag cacggcacac gtgccgcctc tccagtgctt cgggatgaag
     1921 gcgaatcggt aaggagggaa gagaagagag ggagccaaac attcttgggg actgccaccg
     1981 gcaggcaacc gtgggtactg ctgtagctgc ttagtctgcg gtcgccagga actccgagac
     2041 ggtgaccaac tcgtagcccc gctccttgag ggtgcgaagc acctcgggaa gcacctcggc
     2101 agactggacg ctggtcaggt gctggaccag gatcgccccc ggcctcgcgt agcgaagtaa
     2161 cctctccttc accgcggcag cgtcgatccc cggtctccag tcggcgctgt cagcggtcca
     2221 atagatggtg tagtagccct gctggcccgc caactggacc acgcggtcgt cataggcgcc
     2281 ggagggaggc cggaaccagg gacaggtgct ctttccgatg agggcgcaca ccttcacgtc
     2341 cagcccccca actcggcgac catctcctcg tcggtctggc cgggcatgtc ggcgtgggtg
     2401 tcaccgtggt tcccgatctc gttgccctct cgggccatct gcaccaccaa ctccgggtag
     2461 ctgtccacga agtcgccggt caggaatacg gtgcagcgca ctccggcctc ccgaagcgcg
     2521 tctaggatcg ccggttgaaa tggaggatag gaggcggcgt tgaaggtcag ggccaccttg
     2581 ttgctgtcgg tttgcaccac gaggctggga ggcagcggct gccaatcggc cgagggcgtc
     2641 ggcgtggttt cggaaagctg ggacaaacgg ggctgagttt gggcagggtc gaaggcagca
     2701 gtcacctggg gacagagagt cagcaggaga aagacaacgg ccggcagcag caatcgggac
     2761 aagtggcagt ctccaatctg atgtcgatca tctaattgga tgcgatcttg ccacaaacgt
     2821 caactgccgg ccgttgccga cttcgaagta gctgtgcgtt cgatcctccc tacttcccag
     2881 accggtagcc cgccaactct ggccagacgc tggggtcctc ctggccgaga cgccaggccc
     2941 cgacacctcc aaggctgtaa caggtcgcga tctgtagctt gccggcaaca ctggttgcat
     3001 cttccaacca cacctcgtgc tcctgcacgg cgggcgtggg cggtgcaggg cgtggcgtag
     3061 gcgccggacc ggtggccggt ggctgcgggg ttggggccgc aggttgaacc ttgcactgcg
     3121 ccggggcccg aaccgcgatg tcgtgctgca cggccgggac cgccggcggc ggcggtacac
     3181 cgtcgcccgc cttcgcggag tagctgaagg tgccggagcg gctggcgtcg tccgtcgcga
     3241 tctccgtctc gtgttgcttg gcgagggcag cggcctgcgg atagagcaac gacttcgccc
     3301 tcccgcccag cgtcttgttc cagtcgtacc cgtagaaggc gagcccgatc agcaccttct
     3361 ccgcgggcat ctggctcacc gcgtacgccg cgaccttgtc aacccacgtc tgtggcgcaa
     3421 tggagcccgg aggtcccccc ggccaggagt agtcgtaggt catcagcagg atcatgtcgg
     3481 cagacttgcc cagcgccgcg tagtcatagg ggcctgccca gcccgttcga acgtcggaac
     3541 cctttgcggg gatcgccagc gtcagcatct tcccgcgctt gtgcagcgcg tcggccgccc
     3601 tggcgacaag gtccgagaag gcgtccctat cctccgcgtt gatcccctcc aggtcgaggt
     3661 caatgcccgg ataccccatc tcttccacat agctcactat ctgcgtcagg aatcgaaatc
     3721 ggccggcttg aaggggccga ggcgaccagg gtcatagacg ccgcgggcaa gatcgtctgc
     3781 cccgggttca tcgacgtcca cagccacacc gacagcacga tccaggtcaa ccccacggcg
     3841 gagagcacca tccgccaggg taccaccacc gagatagttg gcaactgcgg cagcagcatg
     3901 gcgcccctca ccgatgcctc ccgcgaccgc accacggccc gacttcgcaa gtacggctac
     3961 gacggcccca tcgattgggc gaccttcggc cagtacctgg agtccgtgca acggatgggc
     4021 acttccatca acctcgccca cctggtcggc cacaacgcgt tgcgcggcgc cgccgaggtc
     4081 tccggaccca atgctaccga ggaacagctg cgggccatgg aaggtatggt gcaggaggca
     4141 atggaggcag gtgccctggg gatctccacc gggctggagt tcgagcctgg ccggcaggcg
     4201 cccaccacag aggtgatccg gctggccagg gtggtgggaa ggtacgacgg ccactatgtc
     4261 agccacatcc ggaaccggga cgctcgcgtc caggaggccg tcgaggagtt cctggagatc
     4321 caacgggccg gcgggactcg tggccagatt tcccacctga acacccgcca caacaccggg
     4381 gtgccagagg gaggatggca gcgagccgcg gataccatgg agcaggcccg gcgagatggg
     4441 ctaaacgtcc tggcggacat gacccccttc gccttcggca ccggatccat ggcctccatt
     4501 ctgccgcctt gggtcaggga tgggggaccg acacgaactg cggaactgct tcgcgatccc
     4561 tccgttcgga cccgtctgcg caccgagtgc gaccgctact ggcgcttcat ccatcggggt
     4621 gagtgggatc gtgtgcggct gctgagcagc gccgagtacc ctgagctgtg ctcgaaaacc
     4681 ttcgtcgaga tctcccaact gtgggacaag gatccctggg actgctactt tgacatcctt
     4741 gcggccgccg gtccgaagct ggatgccctc agcctggtcg ccatcctgtt cactgaaggg
     4801 catctaaagg agaccatcag ccatcccctc ttctgcctgg gcgtggacgg ggtcagcagc
     4861 cgaatcgatg gccccctggc tgagcagacc cgcaccccca tcaacttctg cggcatgacc
     4921 cactacctga cctaccacgt cagggagaag cacaccctct cgctggaaga ggcgatccgg
     4981 aagatgacca gcatgacggc gtgccacttc gggctgcgag gccgagggct cctgcaagcc
     5041 gactgcttcg ccgacgtcgt ggtcttcgac ttcgaccggc tggaggacgt atcgaccctg
     5101 gagcagccgg tggcctacgt gcgaggggtg gagcacgtcc tggtaaacgg ggtgccgatc
     5161 atcgacggtg gcaaccatac cggcgcgagg cccggccgca acctgttgcg tggctgaaac
     5221 gaccagtgcc gccttcaccc ccgtgcagca tagtgggatt gaaggcggca cctcagttgg
     5281 aaggcaactg caggtccgaa acagtggtca gttcggggcc ccgtgccttt aggatgcgaa
     5341 gcttctcgct acttcccaga ccggtagccc gccaactccg gccagacgcc ggggtcctct
     5401 tggccgaggc gccaggcccc gacaccccca aggccgtaac gggtcgcgat ctgtagcttg
     5461 ccggcaacac tggttgcatc ttccaaccac acctcgtgct cctgcacgtc gggcgtgggc
     5521 ggtgcagggc gtggcgtagg cgccggaccg gtggccggtg gctgcggggt tggggccgca
     5581 ggttgaacct tgcactgcgc cggggcccga accgcg
//
LOCUS       DATALS010000266         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_102899, whole genome shotgun sequence.
ACCESSION   DATALS010000266 DATALS010000000
VERSION     DATALS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102899"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /gene="ftsH"
                     /locus_tag="VHS06_05900"
     CDS             <1..1295
                     /gene="ftsH"
                     /locus_tag="VHS06_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX2987688.1"
                     /translation="PPQLSNWLGLLSTFLPLIFFGAILVFMMRQAQGSNNQALSFGKS
                     RARMFVGNKPTITFSDVAGVEEAKQELAEVVEFLKYPEKFAALGARIPKGVLLVGPPG
                     TGKTLLSRAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNAPCIVFVDEI
                     DAVGRQRGAGLGGSHDEREQTLNQILVEMDGFDTNTNVIVIAATNRPDVLDPALLRPG
                     RFDRQVVLDRPDIAGRRAILEVHSKGKPMEKEISLDVLAKQTPGFSGADLANLVNEAA
                     ILAARRNKKVISLQEFEEAVDRVIAGPERKSRIISEKEKEITAYHEAGHALVARYLEH
                     SDPVHKISIIADGDVGRLPIRGREHGDKQSDHCHEDDQIYETVTQPLAIHAAPPPQST
                     LGPGETIAQRPWRPRPGSCASRISDLDELYHYLARAKT"
     gene            complement(1329..1853)
                     /gene="hpt"
                     /locus_tag="VHS06_05905"
     CDS             complement(1329..1853)
                     /gene="hpt"
                     /locus_tag="VHS06_05905"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX2987689.1"
                     /translation="MANEVESVLISQEVLQKRIQELGRQLTEEYAGRNLLLLGVLKGA
                     VVFLVDLARAIDLPLQMDFMAVSSYGSSTQSSGVVRILKDLDAAVDGRDILVVEDIID
                     TGLTLKYILENLSMRNPASVKVCALLYKEKARPDGIKPDYVGFEIPDKFVVGYGLDYA
                     ELYRNLPFIGVLKS"
BASE COUNT          406 a          609 c          606 g          379 t
ORIGIN      
        1 acccaccgca actgtccaac tggctcgggt tgctgtccac cttccttccg ctgatctttt
       61 tcggcgccat cctggtgttc atgatgcgtc aggcccaggg tagcaacaac caggcgctat
      121 ccttcgggaa gagccgcgcg cggatgtttg ttgggaataa gcccaccata accttcagcg
      181 atgtggcagg cgtggaggag gcgaagcagg aactggccga ggtggtggag ttcctgaagt
      241 accccgagaa gtttgccgcg ctgggtgcca ggatccccaa gggtgtgctg ttagtgggcc
      301 ctcccggtac tggcaagacg ctcctttcca gggcggtggc cggtgaggcg ggtgttcctt
      361 tcttcagcat aagtggctcc gaatttgtgg agatgttcgt cggcgtcggc gcctcgcggg
      421 tcagggatct ttttgaccag gcgaaacgca acgctccgtg catcgtgttc gtggacgaga
      481 tcgacgccgt gggacggcag cgaggtgccg ggttgggcgg cagccacgat gagcgagagc
      541 agacgctgaa ccagatcctg gtggagatgg acggcttcga caccaacacc aacgtgatcg
      601 tgatcgcggc caccaaccgg ccagacgtgt tggatccggc gctgctgcgc ccgggtcgct
      661 tcgaccggca ggtggttctg gacaggccgg acatcgccgg aaggcgggcg atcctcgagg
      721 tacacagcaa gggcaagccg atggagaagg agatcagcct ggacgttctg gccaagcaga
      781 ctcccggctt ctccggtgcc gacctggcca acctggtcaa cgaggcggcc atcctggctg
      841 cgcggcgcaa taagaaggtc atcagcctcc aggagttcga ggaggctgtg gatagggtga
      901 tcgcgggtcc ggagcgcaag agccggatca tcagcgagaa ggagaaggag atcactgcct
      961 accacgaggc gggacatgcc ctggtagcga ggtacctgga acactcggat ccggtccaca
     1021 agatctccat catcgccgat ggggatgtag gcaggctccc gatcagaggt cgggaacatg
     1081 gtgataaaca gagcgatcac tgccacgagg atgatcagat atacgaaact gttacgcagc
     1141 cacttgctat ccacgcagcc cctccgcctc agtcaacact cggacctggc gaaaccatcg
     1201 cccaacgccc atggcgaccg aggcctggta gttgtgcgag tagaatctca gatttggacg
     1261 aattatacca ttatctggcg cgagccaaaa cctagcccca tccatccggc taatcagccg
     1321 ttgcgtggct acgatttcag taccccgatg aaggggaggt tgcgatacag ttccgcgtaa
     1381 tccagcccgt atcccacgac gaacttgtca gggatctcga acccgacgta gtcgggcttg
     1441 atcccgtccg gcctcgcctt ctccttgtac agcagtgcgc acaccttcac actggcgggg
     1501 ttcctcatgg acagattctc aaggatgtac ttcagagtga ggccggtatc gatgatatcc
     1561 tcgaccacca gaatgtcgcg gccgtccacc gccgcatcga ggtccttcag gatccgcacc
     1621 acaccgctgc tctgagtgct gctcccgtag ctggagactg ccatgaaatc catctgcagt
     1681 ggcagatcta tcgccctggc cagatccaca agaaagacca ccgcgccctt cagcacgccc
     1741 agcagcagga ggtttcggcc cgcatattcc tccgtcaatt gccggcccaa ttcctggatc
     1801 cgcttctgca atacctcttg cgagatcagt acactctcga cttcgttcgc catcgaaccc
     1861 ctctctccaa atgatgaacg gtaaccaccg accgcgtacg ggcatcgcgc ccaatatggc
     1921 tccattggcg gctcggccgc cagtcgggcg ggctagcgag gccaccgggg ccacctcgta
     1981 gggcggggtc ttgtgccccg
//
LOCUS       DATALS010000267         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_419011, whole genome shotgun sequence.
ACCESSION   DATALS010000267 DATALS010000000
VERSION     DATALS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419011"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VHS06_05910"
     CDS             <1..700
                     /locus_tag="VHS06_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2987690.1"
                     /translation="FLAKAVAGEITASFIHLELQAILSMWVGESEQNLHDVFEKAREE
                     KPCILFIDELDALGGSRQKMGQHHERMLVNQLLVELDGLQSYNQGVYVIGATNMPWYL
                     DPALRRPGRFDHLVFVPPPSGEEREQILRLKLKGKPQQPLDLNRIASQAELFSGADLE
                     QLVKDACEAALERSLEAGELQPLSESDLRGALQSRRATTREWFSTARNYAMFSDVNKD
                     YQPVLDYIKENRIK"
     gene            712..1578
                     /locus_tag="VHS06_05915"
     CDS             712..1578
                     /locus_tag="VHS06_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2987691.1"
                     /translation="METYAYYSRVSALLGMERYDEAIREAEEWIQADPEDADAYALMA
                     RAYRYLDKYKEALHWSKEALRLDPENVIGWEVRTGVYYTLEDWPSFRPAVEEALRLFP
                     AEPYFYFLRAGMHSKEGDFKEALADLEKCLELEPEDALYLANYSYMLAMTGEDEKSRE
                     IQKQTLAADPENAYVFLYLGWAADRRGDYREAGEFIKEAIRLEPGDAQIREEYLGILQ
                     KQYRFYRIVLAPIAAYQRLDRAGRILVWIVAWLVFKPFVLLLLLLYFTAHWFTKLLVH
                     VKVFGWNFHWKR"
     gene            1604..>2119
                     /locus_tag="VHS06_05920"
     CDS             1604..>2119
                     /locus_tag="VHS06_05920"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX2987692.1"
                     /translation="MVCRCSWAGEDPLYIDYHDREWGVPVHDERLLFEFLVLEGAQAG
                     LSWITVLRKREAYRQAFDGFDPARVAAYGEEKVAELLNNPGIVRNRRKILSAIRNANA
                     FLKVQEEFGSFDRYLWSFVGGKPKVNAWEQLAQLPARTEESDALSRDLQRRGFNFVGS
                     TICYAMMQAAGL"
BASE COUNT          412 a          578 c          714 g          415 t
ORIGIN      
        1 cttcctggcg aaggcggtgg ccggagagat cacagccagc ttcatccacc tggagctgca
       61 ggccatcctc tccatgtggg taggcgagag tgaacagaac ctgcacgatg tctttgagaa
      121 ggcgcgggag gagaagccct gcatcctttt tatcgatgag ctggacgcgc tgggcggcag
      181 ccgccagaag atggggcagc atcatgaacg gatgctggtg aaccaactgc tggtggagct
      241 ggacggactg cagtcctaca accagggggt ctatgtcatc ggcgcgacca atatgccctg
      301 gtacctggac ccggccctgc ggcggcccgg ccgtttcgac catctggttt ttgtgccgcc
      361 gccttcgggg gaggaacgcg agcagatcct gcgcctgaag ctgaagggga aaccgcagca
      421 gcccctggac ctgaaccgga tcgcctccca ggcggagctt ttctcggggg ccgacctgga
      481 gcagttggtc aaggatgcct gcgaggccgc cctggagcgg tctttggagg ccggggagct
      541 gcagccgctg agcgagagcg acctgcgcgg agccctccag agccgccggg ccaccacgcg
      601 ggagtggttt tccacggccc gcaactatgc catgttcagc gatgtcaaca aagactacca
      661 gccggtattg gattatatca aggagaaccg catcaaatag ggagggatcc catggagacc
      721 tacgcctatt actccagagt gagcgcgctg ctggggatgg agcgatacga tgaggcgatt
      781 cgggaggctg aagaatggat tcaggccgac ccggaggacg ccgatgccta tgccctgatg
      841 gcccgggctt accggtattt ggataagtac aaggaggccc ttcactggtc gaaggaggcc
      901 ctgcggctcg atccggaaaa cgttatcggt tgggaggtta ggaccggagt ctattacacg
      961 ctggaggatt ggccgtcctt ccggcccgct gtggaagagg ccctgcgcct gttcccggcg
     1021 gagccttact tctatttcct gcgggcgggg atgcactcca aggaaggaga cttcaaggaa
     1081 gccctggccg acctggaaaa gtgtctggaa ctggagccgg aggatgccct ctatctggcc
     1141 aactacagct atatgctggc catgaccgga gaggatgaga aatcacggga gatccagaag
     1201 cagaccctgg cagccgaccc ggagaacgcc tatgtctttc tttacctggg ctgggcggcg
     1261 gaccggcggg gggattatcg agaggctggg gagtttatca aggaagccat ccgcctggag
     1321 cccggggatg cacagattcg ggaggagtat ttgggtatcc tccagaagca gtaccggttc
     1381 tatcgtatcg tgttagcgcc gatcgctgcc tatcagcggt tggacagggc ggggcgcatc
     1441 ctggtctgga tcgttgcctg gctcgtcttc aagccctttg tcctgttgct gctgctctta
     1501 tattttacgg ctcattggtt taccaagctg ttagtccatg tcaaggtttt cggctggaac
     1561 tttcattgga aaagatgacg ccatcgacgg aggaggaagg atcatggttt gtcgctgcag
     1621 ttgggcgggg gaggatccgc tctatatcga ctatcacgac cgggagtggg gggtgcccgt
     1681 ccacgacgag cggctcctct tcgaattcct ggtgctggag ggggcccagg ccgggctgag
     1741 ctggatcact gtcctgcgca agcgggaagc ctaccggcag gccttcgacg gctttgaccc
     1801 ggccagggtg gcggcctacg gggaggaaaa ggtggcggag cttctgaata atcccggcat
     1861 cgtccgcaac cgccgcaaga tcctctcggc gatccggaac gctaacgcct tcctgaaggt
     1921 gcaggaggag ttcggcagct tcgaccgcta tctctggagc tttgtcggcg ggaagccgaa
     1981 ggtcaacgct tgggagcagt tggcgcagct tccggcgcgt acggaagaat cggacgccct
     2041 cagccgggac ctgcagcgca ggggattcaa tttcgtcggt tctaccatct gctatgcgat
     2101 gatgcaggcg gccgggctg
//
LOCUS       DATALS010000268         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_384013, whole genome shotgun sequence.
ACCESSION   DATALS010000268 DATALS010000000
VERSION     DATALS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384013"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VHS06_05925"
     CDS             <1..376
                     /locus_tag="VHS06_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987693.1"
                     /translation="AVFLLPELTDYDRAQAKEWLAAQCTPGSVVNIMHERAQQNNEAA
                     RRWLPLWMFAGWHTYKRPIPTAQNPVTAGDPSQFLPDAENPDLVVLDQEQETRNPWSR
                     WERAKRWVVRMVWGGGEQEEYW"
     gene            complement(431..1174)
                     /locus_tag="VHS06_05930"
     CDS             complement(431..1174)
                     /locus_tag="VHS06_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987694.1"
                     /translation="MNWKIIELAGVLAVLMSLVHIFAGRIRFLNVVPRSRWLSMSGGI
                     AVAYVFVHLLPEISRGQSLVIQKGSFTRFFREQHIYLTALAGLVAFYGLERAAKISKP
                     QTPAGQNEALPNKQVFWLHIVSFGLYNMLIGYLLVHREVTGLRSLLFFWFAMSLHFLT
                     NDYDLRQDFKDYYRHVGRWILAAAVLAGWGIGILFALNDVALAFLFAFLAGGVVLNVL
                     KGELPAERESNFWAFVSGVALYTILLLFT"
     gene            1338..>2564
                     /locus_tag="VHS06_05935"
     CDS             1338..>2564
                     /locus_tag="VHS06_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEX2987695.1"
                     /translation="MSSAELRHEDKSRRSFYLAAVVVALGGVLFGYGTAGLSGAILFM
                     SRVFRLSANGVQVVTSLVIIGGILGSAVAGWMGDRYGRRTSLILGAGIFALGSLVTAL
                     SGSIVWIGIGRWITGVGVGITSVNTPLYLSEMAPPDLRGSVVSFNQIALTLGILGAYL
                     VGYALSGTGDWRWMIGVGAGPAVILGIGMVFLPESVRWLVKHGELSRARAALSRFRPS
                     AEVEDELADIQMTMQHHADSLSQLWCPALRIPLVIGVGLAVWQQVTGINAVIFYAPMI
                     LESVGLASAGKALLASSIVGIINVLATVAAAWLLDRVGRRPLLLVGLAGMAVGMAILI
                     LGFRWLGIASFSKPIALGGLVLYVISFAIGLGPVFWLLISSRLRPLVSGTRKYEKTQA
                     AAHRTPYIRNVAGRPTD"
BASE COUNT          521 a          640 c          836 g          567 t
ORIGIN      
        1 ggcggtgttt ctgctcccgg agttgaccga ctatgaccgg gcacaggcca aggaatggct
       61 ggcggcccag tgcacacccg gtagtgttgt gaacatcatg catgagaggg cgcagcagaa
      121 caatgaggcc gctcgtcgat ggctgcctct gtggatgttc gccggatggc atacatacaa
      181 gcgtccgatc ccgacggcgc agaatccagt gacggcagga gaccccagtc agttcctgcc
      241 ggatgctgag aacccggacc tggtggttct tgatcaggag caggagacga ggaacccgtg
      301 gagtcgatgg gagagagcga agagatgggt ggtcaggatg gtctggggag gcggcgagca
      361 ggaagagtat tggtaggggt ggacttgaac gcccgggtgt atgcatcgtg cctgcaaagc
      421 gttggtgggc ttaggtgaag aggaggagga tggtgtagag ggctactccg gacacgaagg
      481 cccagaagtt gctctcccgt tcggccggga gttcgccttt caggacattc agaacgactc
      541 cacccgcaag gaatgcgaag aggaaggcaa gggcgacgtc gttcaatgcg aacaagattc
      601 cgattcccca acctgccaga acagccgcgg cgaggatcca ccgcccgacg tgcctgtagt
      661 agtctttgaa gtcctgacga aggtcataat cgttggtcag gaagtgcaga gacatggcga
      721 accagaagaa gaggagactt cggaggccgg tgacctctct gtgtaccagg agatacccga
      781 tcagcatgtt gtacagacca aaggagacga tgtgcagcca gaacacctgc ttgttcggca
      841 gggcttcgtt ctgtcccgcc ggggtttgcg gtttggagat tttggccgct ctctccagac
      901 cgtagaacgc cacaagcccc gcaagtgctg tgaggtagat gtgctgctcc cgaaagaacc
      961 tcgtgaaact gcccttctgt atcacaagtg actgccctcg gcttatctcc ggaagcaggt
     1021 gcacgaagac gtaagcgacc gcaatccccc cggacatgga cagccatctg ctgcgtggaa
     1081 cgacgttcag aaaacgaatc cttccggcga agatatgcac taacgacatc agtacagcaa
     1141 gaacaccagc cagttcaatg atcttccagt tcatgatcag accgccttcg agaaccctca
     1201 cagacctctt tctaggaagg tgctacacgt cagatgtgct cgttccgggt aacctattct
     1261 tgttatgcgt agagtccggg ctgcataaac ccggtggtta agcatcatca cgggctgtgc
     1321 ggacagggga gattggcgtg agttctgcag aactgaggca cgaggacaag agcaggaggt
     1381 cattctacct cgccgccgtc gtagtggctc taggcggcgt gctgtttgga tatgggacgg
     1441 ccgggctgtc gggggcgatt ctcttcatga gcagagtatt ccggctctct gctaatggcg
     1501 tacaggtggt taccagcctg gtgattatcg gtggaattct cggttcggcc gttgccggat
     1561 ggatgggtga tcgctatgga cgccggacca gccttatact cggagcggga atctttgctc
     1621 tcggctcgtt ggtgacggca ctttcggggt cgatcgtgtg gattggcatc ggtcggtgga
     1681 tcactggtgt cggtgtagga atcacgtcgg tgaatactcc tctttaccta tccgaaatgg
     1741 cgccgccgga cctaagaggg tcggtagttt cattcaacca gatagcgctt actcttggta
     1801 tactgggggc gtatttggtt ggatatgctc tgtccggcac gggtgattgg cggtggatga
     1861 tcggggtggg ggctggcccg gcggttatcc taggtatcgg aatggtgttc ctgccggaga
     1921 gcgtgcgctg gttggtgaag catggggagt tatccagggc tcgggcagct ttgtccagat
     1981 tccgaccttc tgccgaggtg gaggacgagt tggcagatat tcagatgacc atgcagcatc
     2041 atgccgacag cctctcgcag ctttggtgtc ctgcactgcg catcccactt gtgatagggg
     2101 taggtttggc ggtctggcag caggtgacgg ggataaacgc ggtcatcttc tatgcgccca
     2161 tgatcctgga atcggtcggg cttgcatctg caggaaaagc gttgttggca agcagcatcg
     2221 tcgggattat caacgtgctg gctactgttg cggcggcgtg gctgctggac agagtgggtc
     2281 gccgaccact ccttctggtt gggttggccg ggatggcggt cggcatggcg atacttatcc
     2341 tggggttcag gtggttaggc atagcatcat tctcgaagcc tatagctttg ggcgggctgg
     2401 tgctctacgt aatctcgttt gctatcgggc tgggtccggt attctggctt ctcatctcct
     2461 cgagactccg ccccttggtc tccggcacta ggaaatatga gaagacccag gcggcggcac
     2521 ataggacgcc gtatatcaga aatgtcgctg gccggccgac tgac
//
LOCUS       DATALS010000269         6757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_251082, whole genome shotgun sequence.
ACCESSION   DATALS010000269 DATALS010000000
VERSION     DATALS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251082"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VHS06_05940"
     CDS             <1..931
                     /locus_tag="VHS06_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX2987696.1"
                     /translation="DLVSVERALTQAVESEGPTMRRLLGLVLPGSGKRLRPALALVCG
                     RVNDFRPDRLIPFAAALEMLHTATLVHDDIVDEADVRRGAPTLNAHFNNSIAVLVGDF
                     MFAQAADHAVRTEDFRVITRFTRAAMTILGGQIDESWANGSLGMTKEQYFQRIGNKTA
                     ALFALSAEGGAILSGASQPVIEAVKQYGHLMGLAFQIVDDILDVVGDEATLGKPVGSD
                     VRQGTVTLPALMVRDRLTPEQFRAAFNGDGRREEPIRVLLETIRQQGGIELSYREADR
                     LVAEACAALKPLPAGEVRDSLEDLAHFVVRRDV"
     gene            1088..2179
                     /locus_tag="VHS06_05945"
     CDS             1088..2179
                     /locus_tag="VHS06_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HEX2987697.1"
                     /translation="MKVTFPHAGQAWVPLSTVFAKSGVECIVPPPTSKRTLSLGVKYS
                     PEWVCLPFKLNLGNFLEAMEMGADTLAYVSGAGLCRLGYYSKVSEQILRDAGYQFEMV
                     TFDWQDTGIMGLADFLRRVLPDRSLRQIAGDIKFGLSQMKVMDDLERMSHRLRPRADN
                     PVEITRIWRTAGARVAKAWSPSDLKRTKAELIDEFNSVPLNPYADPLRVGLLGEFFMV
                     VESFVNMDVEDRLGRMGVEVHRHAFFGDWARTWLFLEAIGLSHGKKVKKAAAPYLSRD
                     VSGDAVQTVGETILHQQEGFDGIIHLLPFTCMPEIIAQNVMPTVTRDHNIPVLSIVLD
                     EQLGQAGFITRIEAFVDLMNRRRTVKRAS"
     gene            complement(2534..2818)
                     /locus_tag="VHS06_05950"
     CDS             complement(2534..2818)
                     /locus_tag="VHS06_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987698.1"
                     /translation="MSSADYWDPNSIGDRRIGRQGRVKPYSEVQLFFLLRLERLLRVR
                     REHAALSTSNDWWAKLLSKAIYSTYCDCIELGVGEDARSLLSRDQAIAQS"
     gene            complement(2856..3095)
                     /locus_tag="VHS06_05955"
     CDS             complement(2856..3095)
                     /locus_tag="VHS06_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX2987699.1"
                     /translation="MQRAYVLIETLGGKSKEVADALRKVSGIAASDVVTGPYDVIAVA
                     QGADANVIGRLVLNEVRGIPGISKTMTCLAVDSQE"
     gene            complement(3377..4723)
                     /locus_tag="VHS06_05960"
     CDS             complement(3377..4723)
                     /locus_tag="VHS06_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX2987700.1"
                     /translation="MGATRRLSEFAIQSTFDRLPSEVVRESKRCILNYLGVALGAADH
                     QAVRLLMEALAEEGGRQQATILGHGKRCSARQAAMINGTMSHVMDYDDTHLPTVIHPT
                     GPVLSAALATAEWKGLSGRELVAALAAGMEVELRVGAAVYPSHYDVGWHITGTAGTFG
                     AAAAAGRLLGLGVEEMEWAMGMAGSQAAGLREMFGSMTKALHVGKAASNGLLSAMLAN
                     RGFTASPEVLEGRRGFCAVTASEYDLGQATVGLGDEYLMLQNGIKPYACGVVTHPTID
                     GVRKLRERFRLQPDDVLEIQLKVHPLVRELTGKKSPRTGLEGKFSVYFCAAIALIEGN
                     ARESQFTDEKVNRPDVVSMMDRVSAHVEPGVLEEQAIVSIRTVDGRVLVERVEAATGT
                     PDNPMPDDQLMEKYMELATRTLSRERAEQLAEQVDRLEQMEELSLLIGTATNRRRK"
     gene            4949..5902
                     /gene="xerA"
                     /locus_tag="VHS06_05965"
     CDS             4949..5902
                     /gene="xerA"
                     /locus_tag="VHS06_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HEX2987701.1"
                     /translation="MKQQVNAFLGYLAAERGLAANTLAAYNNDLNQFAEYLSSRDQTG
                     LELSGSAGGRQDDQGALPDMGRDSVMGFFLHLREKGYTPSTIARKTAAIKSFFHFLAS
                     KGYVSEDPTASIDSPRISKSLPRAASVEEIDELLEAPTKVASPEAMRDKAMFELLYAT
                     GMRVTELVSLNLGDVDIAQGSVQCAGRSAGKGARLRVIPVHATAIKAVHDYLDNARPM
                     LVREGDEMALFVNHRGDRLTRQGFWLILKQWAKEAGITTQITPHTLRHSFALHMLRNG
                     VDLRAVQQLLGHAHISTTQIYTYPSEDGLKRLYETAHPRAK"
     gene            5903..6628
                     /locus_tag="VHS06_05970"
     CDS             5903..6628
                     /locus_tag="VHS06_05970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2987702.1"
                     /translation="MAGVEEHPIGEPVPAFSAVLFDAGNTLIYMPQTAEEILMDLCHQ
                     LGAPIDLDAARSAYHQSEQFYARNYLGYRGDQGSFWHRYHGEALRYLGIDDPDGRKAA
                     FLSHSFGQIGIWQPYPEAGRVCSTLQAMGVRMGVVSNGSTTVSDLLSQAGLLPFLETV
                     VDSQSIGIQKPDVRIFHEALGRMGLASGDALFVGDLYDVDVVGARAAGMKAVLIDHDG
                     DGADWDCPVIHTLDELIPLVARS"
BASE COUNT         1239 a         2091 c         2056 g         1371 t
ORIGIN      
        1 tgatctggtt tcggtcgagc gagctctcac tcaggcagtg gagtccgagg gcccgaccat
       61 gcgacgactg ttgggcctgg ttcttccagg cagcggcaag cgccttcgcc ctgccctcgc
      121 cctggtctgt ggacgggtaa acgattttcg gccggatagg ttgatcccct ttgccgccgc
      181 cctggagatg ctccacactg cgacgttggt ccacgacgat atcgtggatg aggccgatgt
      241 gagacgggga gccccgactc tcaatgctca cttcaataac tctatcgcgg tcctggtcgg
      301 cgatttcatg tttgcccagg cagccgatca tgcggtcagg accgaggact ttcgagtgat
      361 cacccgcttc actcgagcgg caatgaccat ccttggcggc cagatcgacg agtcctgggc
      421 taacggcagc cttggtatga ccaaggaaca gtacttccag cgaatcggca acaagaccgc
      481 cgcgctgttc gccctgtccg ctgagggcgg cgcaatactc agcggcgcct ctcagccggt
      541 aatcgaggcg gtgaagcagt acggccacct catgggattg gctttccaga tagtcgacga
      601 catcctggac gtggttggcg atgaggcgac cctgggcaag ccggtaggga gcgacgtgag
      661 gcagggaacg gtgacgctac cggccctgat ggtgcgcgat cgcctcacgc cggagcagtt
      721 tagggcggcc ttcaacggcg acggacgcag ggaagagccg ataagggtgc tgctggagac
      781 gatccggcag cagggcggca tagagttgtc ataccgggag gcagataggc ttgtggccga
      841 ggcttgcgcc gcactgaagc cgctgccagc cggcgaggtc cgcgactccc tcgaagattt
      901 ggcgcacttc gtcgtccgcc gcgacgtgta acgcaaaagg gttccactca acaggataca
      961 taggatcgat tggatagaca ggatcgagaa agggcccccg gcgcatcctg cgcatccctg
     1021 gtcatcctgt ccatcctgtt tcgttttttc ctccacgctg gctgtcaatc gctaatggag
     1081 aagtctgatg aaggtaacct tcccccacgc cgggcaggcg tgggtccccc tgtctaccgt
     1141 cttcgcgaag tcgggtgtgg agtgtatcgt cccccctccc accagcaagc gcaccctctc
     1201 ccttggagtg aagtactccc cggagtgggt ctgcctgccc ttcaagctga acctcgggaa
     1261 cttcctcgag gcgatggaga tgggcgctga taccctggcc tacgtgtccg gcgcggggct
     1321 ttgccgcctg ggctactact ccaaggtgtc cgagcagatc ctgcgggatg ccggctacca
     1381 gttcgagatg gtcaccttcg attggcagga cacggggatc atgggactgg ccgatttcct
     1441 ccgcagggtg ctgcccgatc gttcgctgcg gcagattgcc ggagacatca agttcggtct
     1501 ctctcagatg aaggtgatgg acgacctgga gcgcatgagc cacaggctgc gcccgagggc
     1561 ggacaaccca gtcgagatca ctcgcatctg gcgaaccgcg ggggctcgtg tagctaaggc
     1621 gtggtcgccg tcggacttga agcggacgaa ggccgagttg atcgatgagt ttaactcggt
     1681 accgctgaat ccctacgccg atccccttcg tgttgggttg ctgggagagt tctttatggt
     1741 ggtcgagtcc ttcgtgaaca tggacgtgga ggatcggctc ggacgcatgg gggtggaggt
     1801 gcatcggcat gccttctttg gagactgggc gcgcacctgg ctcttcctgg aggctatagg
     1861 gctcagccat gggaagaagg tgaagaaggc cgccgccccc tatctcagtc gcgacgtcag
     1921 tggcgatgca gttcagaccg tgggagagac catccttcac cagcaggaag ggtttgacgg
     1981 cattattcac cttttgccct ttacctgcat gccggagatc atagctcaga acgtgatgcc
     2041 cacggttacc cgggatcaca acatccctgt gctgtccata gtgctggacg agcagttggg
     2101 gcaggccggc ttcataaccc ggatcgaggc atttgtggac ctgatgaacc ggcgacgaac
     2161 ggtcaagcgg gcaagctgac cccatcttgg agtgtggcgg ctcccgcgtg gaggctgccg
     2221 aacagggcga aacgggccac cgttttggag tgcggcggct tgacgccgca aggtggggcg
     2281 gagtcgcgtg gaggacggcg gcaaacgcga tcccctgatg gcaaggtgtg ttgggcgaca
     2341 ttgcggcgtc aagccgccgc actccagaaa gacggccacc gccagggcaa tcgcaacata
     2401 gcgcaaatcg atagaggctt cgaccggaag aacgacgatc cggccgaagc ctctactcaa
     2461 tttgtggtca gttcggtagg tttcttggag atgtgcgggg cgacagaggg tccagagggt
     2521 tcgaagcagg gcgtcagctc tgagctatgg cctggtcccg ggaaagcagg ctgcgggcat
     2581 cctcacccac tcccagttcg atgcagtcac agtaggtcga gtaaatcgcc ttgctcagca
     2641 gctttgccca ccaatcgttg gatgtggata gggccgcatg ctcacggcgc acacgcagca
     2701 gccgctccag ccgcaggagg aagaacagtt gaacctcgct gtaaggcttg actctgccct
     2761 gccgtccaat cctccgatca cctattgagt tcggatccca ataatcagcg ctactcatct
     2821 cagttctccc cagcaggccg ctgccggttc tggccctact cctgcgagtc cacggccagg
     2881 caggtcatcg tcttgctgat gccgggaatg cctcggacct cattcagcac cagccgtccg
     2941 atgacgtttg cgtcggcgcc ctgggccacg gcgattacgt cgtacggccc ggtcaccaca
     3001 tcggaggccg caatgccgga cacttttcgc agcgcgtccg ccacctcttt gctcttgccc
     3061 cccaacgttt cgatcagaac gtaggctctt tgcatcgccg ttctacctcc gctgcagcgc
     3121 tcgattccgc gagccgatgg ccatcaccac catcctcata ttaggctttc cagcgacgat
     3181 gtcaagacct tttggcccct ccagcagtgc acttggcgtg ccgaacgtcc agaattgcct
     3241 gcaactcagt gtagctcgta gtagtgcgct ttccagtatt cgcttctggc ccatgatgtt
     3301 cgtgaagcgc ttacaatagc taaccagcag taagcatgaa taatgatgtt gttgcatttt
     3361 cgggggccgg cttcgcctat ttccgccgtc ggttggtagc tgttccgatg aggagagaca
     3421 gctcctccat ctgctctaga cggtcaacct gctccgccaa ctgctcggcc ctttcccggg
     3481 aaagcgtcct ggtggccagc tccatgtact tctccatcag ctggtcgtcg ggcatcgggt
     3541 tgtccggggt tcctgtagcg gcctcgactc gctccaccaa cacccggccg tccactgttc
     3601 gaatggacac tatcgcctgc tcttccagta ctcccggttc cacatgcgcg ctgaccctgt
     3661 ccatcatgga tacgacatcg ggccggttca ccttctcgtc ggtgaactgc gactcccgcg
     3721 cgttgccctc gatcagcgca atggcggcgc agaagtagac gctgaatttg ccctccagtc
     3781 ctgtgcgagg ggatttcttg ccggtgagct ctcgcaccag cgggtgaacc ttcaactgta
     3841 tttcgagcac gtcgtccggc tgcagtcgaa aacgttctct cagcttgcgc acgccatcaa
     3901 tggtcgggtg cgtcacgacg ccgcaggcgt agggcttgat cccgttctgg agcatcagat
     3961 actcgtcacc caaccccacc gttgcctgcc ccaggtcgta ctctgatgcg gtcaccgcgc
     4021 agaagcccct tcgcccctcc agcacctccg gagatgcggt gaatcccctg ttggccagca
     4081 tagccgagag tagaccgttc gaggccgcct tgcccacatg cagcgccttc gtcatagaac
     4141 cgaacatttc ccgaagcccg gcggcctggc tcccggccat ccccatggcc cactccattt
     4201 cctccacgcc cagtcccagc agcctgcctg ccgccgcggc cgcgccgaag gtgccggcgg
     4261 tgccggtgat gtgccatccc acgtcgtagt gggagggata gaccgccgct cctaccctca
     4321 actccacctc catgccggcg gccagcgcgg cgaccagctc tcgcccgctc aaccccttcc
     4381 actcggccgt cgccagggcg gcggagagta ccggtccggt gggatggatg acggtgggaa
     4441 gatgggtgtc gtcgtaatcc attacatggg acatcgtccc gttgatcatt gctgcctgtc
     4501 tggccgagca gcgcttgcca tgccccagga tggtagcctg ctgtcttccc ccttcctcgg
     4561 ccaacgcctc catcaatagc cggactgcct ggtgatcggc ggcgcccaga gccaccccca
     4621 ggtagttcag aatgcacctc ttggactctc gaacaacctc ggaggggagt ctgtcgaagg
     4681 ttgactggat ggcgaactcg gacagccggc gggtagcgcc cataagatga tgcctccttc
     4741 tatacattca attcggcacg accctgcctc aggattaggg ttacgcgaaa ggtttccgac
     4801 aaagctggcg tgggtggcac cagaagaaca gatgctacta taatggaaag ctccgaccgg
     4861 tctccggtcg cgtggcgtga cttttcgcaa caccttatgg taaactcgcg cgtcacttca
     4921 gtcgtttcgg cggtggaggt tgtccctcat gaagcagcag gtgaatgcct ttctcggata
     4981 tctggcggcc gagaggggtt tggcggccaa tacgctggcg gcctacaata acgatctcaa
     5041 ccagttcgca gagtacttat cttctagaga ccagaccggg ctcgaattat ccggctccgc
     5101 gggcggccgc caggatgatc agggggcgtt gccggatatg ggtagggact ctgtgatggg
     5161 tttctttctt cacctgcgag agaagggcta tacaccctct acgatagcca ggaagaccgc
     5221 cgccatcaag tcctttttcc atttcctcgc ctccaagggc tacgtttccg aggaccccac
     5281 cgccagtatc gattccccga ggatcagcaa gtcgctcccg cgggccgctt cggtagagga
     5341 gatcgacgaa ctgctggagg ctcctaccaa ggttgccagc cccgaggcaa tgagggacaa
     5401 ggcgatgttc gagttgctgt atgccaccgg catgcgcgtc accgagttgg tctcgctgaa
     5461 cctgggcgac gtggatatcg ctcagggctc cgttcagtgc gccgggcgca gcgccggcaa
     5521 gggagccagg ctgcgggtga taccggtgca tgctacggcg atcaaggcgg tgcatgatta
     5581 tctggacaac gccaggccga tgctggtgcg cgagggcgac gagatggcgc tcttcgtcaa
     5641 ccatcgtggg gaccgcctga ctcgccaggg tttctggctg atcctgaagc agtgggccaa
     5701 ggaggcgggc ataaccacac agattacccc ccacaccctg cgccactcct tcgcgctgca
     5761 catgctgcgc aacggcgtgg accttcgggc ggtgcagcag ttgctggggc acgcccacat
     5821 ctcgaccacc cagatctaca cctaccccag cgaagacggc ctgaagcgcc tctacgagac
     5881 cgcccatccg cgggcaaagt agatggctgg ggttgaggag caccctatag gggagcccgt
     5941 tccggctttc agcgcggtcc tgttcgacgc ggggaacacc ctcatctaca tgccccagac
     6001 tgccgaggag atactgatgg atctctgcca tcagttgggt gcccctatcg acctcgacgc
     6061 tgcccggtcc gcctaccacc agagtgagca gttctacgct cgcaactacc tggggtacag
     6121 aggtgaccag ggttctttct ggcatcggta ccacggtgag gcgcttcgct accttgggat
     6181 cgacgacccc gatgggcgga aagcggcctt tctatctcac tccttcgggc agatcggcat
     6241 ctggcaaccg taccccgagg ccggacgcgt gtgctccacg cttcaggcaa tgggggtgcg
     6301 aatgggtgtc gtctccaacg gttccacgac cgtgtcggac ctgctttctc aggccggact
     6361 tctccccttc ctcgaaaccg ttgtcgattc ccaatcgatc ggcatccaga agccggatgt
     6421 gcggatcttc catgaggcgt tgggtcggat ggggctggcc tcaggtgatg cactgttcgt
     6481 aggggacctg tatgatgtgg acgtggtggg cgctcgtgcc gcggggatga aggccgtgct
     6541 gatcgatcat gacggcgacg gcgcggattg ggattgcccg gtaatccaca ccctggacga
     6601 gttgatcccg ctggttgcac ggtcgtaggg cggggtcttg tgccccgcct cccacaccgg
     6661 ggttgcgacc acgatggtga ctccggccac gctctaggcg gggcacaaga cgcgtaatgt
     6721 acatcgccga ctcgatccgc catcttttgg aacacac
//
LOCUS       DATALS010000270         6062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_161196, whole genome shotgun sequence.
ACCESSION   DATALS010000270 DATALS010000000
VERSION     DATALS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161196"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            602..1630
                     /locus_tag="VHS06_05975"
     CDS             602..1630
                     /locus_tag="VHS06_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2987703.1"
                     /translation="MTDTLRDVALHAGVSISTASRVLSGSSHPVAADARERVLRAAEE
                     LGFTPNAFARGLSKREFRLIGLVIPDMRDPYFVEIARGAEELANQRGYLTILCNTNRE
                     PDKERSYVQTLRAMRAGIILTGGGIDREAHLRDLATHPAPVVVIGRHALPCSSVLIDN
                     LRGAMDATAHLIGLGRRRIAFIGGPSSSATAADRLVGFRRAMEENGLSIDEDLVVDGD
                     FSLEGGARAARALLRQTHAPDAIFAANDEMALGVMREARHQGIRIPEELAIVGFNGIA
                     ATAETDPPLTTIQLSLGRIGQIATDLLLKQVEKKEKEQVAVTIKGELVVAGSTVPQCE
                     ELHCAKRM"
     gene            1864..2625
                     /locus_tag="VHS06_05980"
     CDS             1864..2625
                     /locus_tag="VHS06_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2987704.1"
                     /translation="MPYFTNNGHRLHYREAGSGPLLLLLHGNTASSVNHEGELAHFCD
                     RFHVVALDFLGTGLSDRMKEWPDDWWAQGARDGAVLAEHLGYERTFVAGTSGGGIAAL
                     QMAIFFPDLVDAVIADSCPAHTTPEERRNEVAFRSRRSPRQIEFWHRAHGDDWEEVVE
                     SDSQFLLRTAERNEDPLLGRLSSIRCPVLFTGSFRDKSIPTLDTQICGMAKEIPGSQI
                     FMSNDGAHPLMWSAPNIFHRIADPFLDSLVRAAVS"
     gene            complement(2698..3681)
                     /locus_tag="VHS06_05985"
     CDS             complement(2698..3681)
                     /locus_tag="VHS06_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX2987705.1"
                     /translation="MDFLVIALPTEDSLPSASKELIGGAKGIVSSSGGTVSVAVLGKG
                     GAEACREAAAFGADRAYLVESSRLVDYDAEAYLQALEIVVRESKPSVVLFTADAFTSE
                     LAPRLAHRLGTGAATGCAGMRVAEKGQMAFTAPVFGGKAMAEMSITGSPQIATVAGGS
                     LEALERDDTRQIETTAIELPAETSARATKVLERRKEEVEGVRLEDAKVIVAGGRGLGE
                     AANFRYVEQLAQVVGGAVGASRPAVDAGWVPPNMQVGQTGKTVCPDLYIAVGISGASQ
                     HLAGMGHSKHIVVINKDPEAPFFKVAELGVVADYKKVVPILTEKLRGLCSK"
     gene            complement(3722..4522)
                     /locus_tag="VHS06_05990"
     CDS             complement(3722..4522)
                     /locus_tag="VHS06_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX2987706.1"
                     /translation="MHIAVCINHIPDPEAPAKQFKLDEQTNRPLLQRANRVVGPFDEN
                     ALETALQLKDLHGAKVTALTVGPASNVEALRRALATRCDAAVHVKEDGASELDSLGIA
                     RLLKAAVQKLGDVDLVLCGRQVGDWDSGQVGQLLAEEMGIPCVTLARQIEPSGESALR
                     VTREVPGGTALVESQLPAVLTVTNAPTNQLRIPKVKDVMLAHRAPITTWTSADLGIDM
                     SELAGGARVVVGRMFIPESSIQVELIRGETDEEVAETLAQRIVGLKVI"
     gene            complement(4533..4988)
                     /locus_tag="VHS06_05995"
     CDS             complement(4533..4988)
                     /locus_tag="VHS06_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014933919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX2987707.1"
                     /translation="MIIHKSMDDLQVGDSACFSKTVTETDALMYVGATGDFSPIHVDE
                     QYSQGTRFGHRIAPGIMVAGMITAVLGNDLVGCDPISVGDEFRFKAPIYYGDTITAEM
                     RIVEKIPEKRVLKLEATCTNQNDVVVLTAKAVMVFPKPVPAPSTGGGQA"
     gene            complement(5053..5493)
                     /locus_tag="VHS06_06000"
     CDS             complement(5053..5493)
                     /locus_tag="VHS06_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX2987708.1"
                     /translation="MTYHFVEKTIDSFNVGDSYCFSKTVTEADVVMFAAVSGDMAPQH
                     VNAEYAKGTPSGERVAHGMLTASLVSAALNRMLSPGATIKSCEFSFAGPVRFGDTINA
                     RVEVIEKHPSSRQLKLRVVCTNQREEIVLEGTSVQVMAGRAAIS"
     gene            5844..6023
                     /locus_tag="VHS06_06005"
     CDS             5844..6023
                     /locus_tag="VHS06_06005"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX2987709.1"
                     /translation="MTPHVGAVLYVAAGIGKKTIEDVSKNLRSFFGVLVLALFVITYL
                     PDVTLWAPRMFGYEG"
BASE COUNT         1172 a         1920 c         1750 g         1220 t
ORIGIN      
        1 actgccgcgc catgtcccgg atcatcttct gggtttcggt tggctggaaa tccatctcct
       61 ctggatcctc ctcctcgaat tgtatcggtg tgcgggaaag cagtcggaag cgccgcttcc
      121 aatcgagatc actcggcgcc ccaaagagtt aggcaaccgg gtgccattat acacacttcg
      181 gcccccactg tccgtccaat tccaaaccag gccgccgatc tcacggctct catccgcggc
      241 cccgaggatc cgatggacgc ggctaagcgg ctgtcgtcac agcattcgat caacacccga
      301 ccataggtgt atggcgtcta ccccacggtc gcgatgcata tcctgatcgt catgcgaagt
      361 accgggttga gatacttggt tagaatggct cggtgggcta tactcatgta gaggtagaag
      421 ctcgggagaa atgtgcagac gtatggacat ggagtcttga gaggtggcgc ggcatagggt
      481 agaatggaat ggcaatcggt tgccgttgcc gcgatcggcg ggagccaaca acctccatct
      541 cgcggatcag cgcctcccgt agctaggagc catcaacttt gattgagggt ggccaataac
      601 gatgaccgat actcttcggg atgtcgccct gcatgcaggg gtctcaataa gcaccgcttc
      661 ccgagtcttg agtggaagca gccatcccgt agctgccgac gcgcgtgaac gcgtcctgcg
      721 agctgcggag gagttggggt tcaccccaaa cgcatttgct cgtggactct ccaaacggga
      781 attccggttg atcggcctgg tgatccccga catgcgcgac ccttactttg tcgaaatcgc
      841 ccgcggtgcc gaggaattgg ccaaccagcg gggctatttg accatcctat gtaacaccaa
      901 ccgcgagccg gacaaggaac gcagctacgt gcagaccctc cgggcgatgc gagccgggat
      961 tatactgacc ggcggcggga tcgatcgtga ggcacacctc cgggacctgg cgacccaccc
     1021 cgctcccgtg gtggtgatag gtcgtcacgc gctaccctgc tcctcggtcc tcatagacaa
     1081 cctgcggggt gccatggacg ccactgccca cctcatcgga ctgggccggc gacgaatcgc
     1141 ctttatcggc ggccccagca gttccgccac ggcagccgac cgcctggtgg gttttcgccg
     1201 agccatggag gaaaacggcc tgtccatcga tgaagacctc gtggttgacg gggatttctc
     1261 cctggagggc ggcgcccgcg cggcccgagc actgcttcgg cagactcatg ccccggacgc
     1321 gatctttgcc gccaacgatg aaatggcact gggagtaatg cgcgaagcga gacaccaggg
     1381 gataaggatc ccagaagagc tggccatagt tgggttcaat ggcattgcgg ccacggccga
     1441 gaccgaccca ccgctgacga cgatacagct ctctctcggt cggatagggc agatcgcgac
     1501 agacctgctg cttaaacagg ttgagaagaa ggaaaaagag caggttgccg tgaccatcaa
     1561 gggcgaactc gtggtagcag gatccaccgt gccgcaatgc gaggagctgc actgcgcgaa
     1621 gaggatgtga ccggtccccg tccccggtag cccattggct ctcttcactt cggcatctgg
     1681 ctcagtcctc acctgatcca gtttcaccag gtccagatca ccgctccctg tcgagggaac
     1741 cgctcgaagc ggcccctccg gtgaagtgct gcctgcgagt tgtgtggggc gaacgtgtgg
     1801 gaaacggctg ccaggcggtg agatcacggt cgtgacgata agaggaggtc caatagcata
     1861 acaatgccat acttcaccaa caacggccac agactccact atcgagaggc gggaagtggc
     1921 ccgttgctcc tgctgctcca cggcaacact gcttcatccg tcaaccatga gggggagctg
     1981 gctcatttct gcgatcgatt ccacgtggtc gcgctggact tcctggggac agggctgagc
     2041 gatcggatga aagagtggcc cgacgattgg tgggcccagg gtgcccgaga cggggcggtg
     2101 ctggcggagc acctgggcta cgagcgaaca ttcgtggccg gcaccagcgg cgggggcatc
     2161 gccgccctcc agatggcaat cttcttccct gatctggtgg atgcagtcat cgccgattct
     2221 tgccccgcgc acaccactcc tgaagagcgc cgcaacgagg tcgcgttccg atctcgccgg
     2281 tcaccccgcc agatcgagtt ctggcatcgg gcacatgggg acgactggga ggaggtggtg
     2341 gagagcgaca gccagttcct gctccgaacc gcagaaagga acgaagatcc tcttctcggc
     2401 cgtctttcct ccattcgctg tccggtgctg ttcaccggca gcttccgcga caagtccata
     2461 ccgaccctgg acacgcagat ctgtggcatg gcaaaggaaa tccccggcag ccagatcttt
     2521 atgtcaaacg atggggcaca tccactaatg tggagtgccc cgaacatctt ccaccggatt
     2581 gcggacccgt tcttggactc tctggtgcga gcggcagtct cgtaattggc caaaagtgga
     2641 gagccggcgg cgccggctct ccactacttc ggactgcacg agagatagcg tggttactta
     2701 cttagagcag aggccacgca acttctcggt caggataggc actaccttct tgtagtcggc
     2761 tacgaccccc agttccgcca ccttgaagaa tggcgcctcc gggtccttgt tgatcaccac
     2821 gatgtgcttc gagtgcccca tccctgccag gtgctggctc gcccccgaga tgcccaccgc
     2881 tatgtagagg tcagggcata ccgtcttacc cgtctgcccc acctgcatgt tgggtggcac
     2941 ccatccggca tccaccgcgg ggcgggaagc gccgacggca ccacccacaa cctgagccaa
     3001 ctgctcgaca taccggaagt tggcagcctc acccagccca cggccacccg ccacgatcac
     3061 cttcgcgtct tccagacgaa ccccctcgac ctcctctttg cgtcgctcga ggaccttcgt
     3121 tgcccgagcc gatgtctctg ccggcaactc tatggccgtc gtctcgatct gcctcgtgtc
     3181 gtcgcgctcc aacgcctcca gactgccacc tgccaccgta gctatctggg gagagcctgt
     3241 tatcgacatc tccgccatgg cctttccacc gaagaccggc gccgtgaatg ccatctgtcc
     3301 cttctcggcc actctcatcc cggcgcagcc cgttgccgcc cccgtcccca atcggtgcgc
     3361 caacctcggg gcaagctcgg aggtgaaggc atcggctgtg aagaggacga ccgaaggctt
     3421 gctctctcgg accacgatct ccagtgcctg caggtaggct tccgcatcgt agtcgacgag
     3481 tctgctgctt tctaccaggt aggctcggtc cgcaccaaag gctgctgcct cacgacacgc
     3541 ctcggcgccc cccttcccca ggacggcgac cgacacggtg ccccctgagc tggagacaat
     3601 ccccttcgcg cctccgataa gctccttgga cgcggatgga agcgagtctt ctgttggcag
     3661 tgcgattacc aagaaatcca ttgcgattct ccagagtctg gtctagacct cttcaattcg
     3721 actagatgac cttcagtccg acgatacgct gggccagagt ctccgctacc tcttcgtcgg
     3781 tttcgccccg gatcaactcg acctgtatgc tgctctcagg aatgaacatg cggcccacga
     3841 cgactcgagc gccaccggca agctccgaca tgtcaatgcc caggtcggcg gaagtccacg
     3901 tggtgatcgg cgcccgatgc gcgagcatca catccttgac cttggggatg cgaagctggt
     3961 tcgtcggtgc attggtgacc gtcagcaccg ccggcagttg actctccacc agggcggtgc
     4021 cgcctgggac ctcccgggtt actcgcaggg cactctcgcc gctcggctcg atctgccgag
     4081 ccagcgtgac acatgggatc cccatctcct ctgccaacag ttggccaacc tgcccgctgt
     4141 cccagtcccc aacctggcgg ccacacagaa ccagatccac gtcccccaac ttctggacgg
     4201 ctgccttgag gagacgagcg atacccagcg aatccaattc cgaggcgccg tcctccttaa
     4261 cgtgcacggc agcgtcgcag cgagtcgcga gagcgcgtcg cagggcctcg acgttggatg
     4321 ccggccccac cgtcagtgcc gtcaccttgg ctccgtgcag atccttcaac tggagggccg
     4381 tctcgagagc gttctcgtcg aaaggcccaa ccaccctgtt cgcccgctgc agcagcggcc
     4441 tgttcgtctg ctcgtccagc ttgaactgct tggctggagc ttccggatcg ggaatatgat
     4501 tgatacaaac tgcaatatgc atggaatgcc tcctacgctt gccctccgcc cgtcgaaggc
     4561 gcgggcacag gctttgggaa aaccataacg gccttggcgg tcaacaccac cacgtcgttc
     4621 tgattggtgc aggtggcctc gagcttgagg accctcttct cggggatctt ctcgacgatt
     4681 cgcatctcgg ccgtgattgt gtcgccgtaa tagatcggcg ccttgaatcg gaattcgtct
     4741 ccaacagaaa tcggatcgca gcctacaagg tcgttcccca gcacagcggt gatcatccct
     4801 gccaccatga tgcccggagc gatgcgatgg ccaaacctgg tcccctggga gtactgctcg
     4861 tcaacatgga tcgggctgaa atctccagtg gctcccacgt acatcagtgc atcggtctcg
     4921 gtaaccgtct tggagaagca ggccgagtcg ccgacctgga gatcgtccat ggatttgtga
     4981 attatcactt ctacccccta cagtcctgga agtcgattgc cgagtcggac tatgtgttgc
     5041 ggccccatcg gctcagctta tcgctgctcg ccccgccatg acctgcactg acgttccttc
     5101 gagaactatc tcttctctct ggttggtgca tacgactcgc agcttgagct gtctgcttga
     5161 cgggtgcttc tcgatcacct ctaccctagc gttaatcgtg tccccaaatc gtaccggacc
     5221 agcaaaactg aactcgcacg acttaatggt tgccccaggg gagagcatcc ggttgagggc
     5281 cgcagaaacc agggaggcag tcagcattcc gtgggcgacc cgttcaccgg agggcgtgcc
     5341 cttggcatac tccgcgttga catgctgagg ggccatgtca cccgagactg ccgcaaacat
     5401 cacgacgtcg gcttcagtga cggtcttgga gaaacagtag ctgtcgccaa cattgaagct
     5461 atcgatggtt ttctccacga agtggtaggt catacgaaac ccctttcaca actggtgctg
     5521 tggcactggt agctggccat cgaggaggga cgcgccggat gccaaatggc aaccgctttc
     5581 catcagcggt agcgccatga tagattggat cattcgccct gtcaatgcgc cgcgctcacc
     5641 cgctgatgac tcggcagaca ggtcacccaa ctccaccaaa aagcattggc gaagaagtca
     5701 cgtttcattg acacggtagg acggccgaat tatcatgtcg tcgccgtggc aaccgcttgc
     5761 ccttgggcgc cttctgcgtc gggcagtcca ccttccacgg cggttcgccg gttcaccggg
     5821 tttcgtcgag taccgacgct gtgatgacgc cccatgttgg agctgtcctt tacgtggcgg
     5881 ccggcatcgg gaagaagacc atcgaggatg tcagcaagaa tctgcgatca ttctttgggg
     5941 tgttggtact ggctctgttt gttatcactt atctgcccga cgtcaccctc tgggccccca
     6001 ggatgtttgg gtacgaggga tagtgggatt agatcggcag tggaggggag tgtttaaaga
     6061 tg
//
LOCUS       DATALS010000271         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_27861,
            whole genome shotgun sequence.
ACCESSION   DATALS010000271 DATALS010000000
VERSION     DATALS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27861"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VHS06_06010"
     CDS             <1..236
                     /locus_tag="VHS06_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2987710.1"
                     /translation="ASIKKTGRLVTVEESRVCGGWGAELAARVATDGFDSLDAPIQRI
                     GAPDVPIPSSPALEKLYVPSEEQIVAAVRKTLQ"
     gene            451..2538
                     /locus_tag="VHS06_06015"
     CDS             451..2538
                     /locus_tag="VHS06_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX2987711.1"
                     /translation="MTRSGIDAFLKPRSIAVLGASAHEGKIGNIVVRQLAGKGFTLYP
                     VNPKGGSVEGIPILRSLREAPEAPELAVLTLPAEAAVPAAAECVERGVRGVIAIAGGF
                     GETGEEGRKLEAQLADVIKGSHTRLLGPNTLGVLSPGTGLDTVFLSPERFQRPGAGAL
                     ALISQSGSAVLGELDNQALHGITISAFVGLGNRLDVDEEEWIRYFADDPASTVIGLYL
                     ESFSDATQLLDTCERVVATKPIVLLKAGCSESGARAVQLHTGSLAGSDQVTDGALRQA
                     GILRVRDCEELLDVGRTLAYARPLEGRNVAVLTNGGGWGILAADCIESKEWGAGLRLA
                     RLTPETTGRLGKVTLPYAALRNPVDVTASADNKMFADALEILQDAPEVDAVLCTFGFQ
                     PPAIDERLTDVLCHYGKSGRKPLVVSLMGSESAMHSLRRLEATGVPAYPSTKRAVRAI
                     AALAQRGEYLRRRRDGTQTGIAAIRSRGPEIDDQFRPGTPLAEDEVKALLRARGIATP
                     NGVVLDRGLLPETLYLAYPLALKVRSAAILHKTDRGGVMLDIKDRPQLETAVEEMRSR
                     FPGEDLLAEEMEPKGVELIVGLISDPTFGLSIMCGIGGVLAELYRDVTFRRLPIDRID
                     ADEMIGELNAHAILEGFRGIKASREAVVELLLRVSALGVELGEGVDQLDLNPVIVHED
                     GAVVVDAKLLWKW"
     gene            complement(2561..3103)
                     /locus_tag="VHS06_06020"
     CDS             complement(2561..3103)
                     /locus_tag="VHS06_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX2987712.1"
                     /translation="MALIPYRGVMPKIAEGVFIAEGAQIIGDVTIGAGSSVWYNTVIR
                     GDVASVTIGQNVNVQDNSTVHVGRGVPCVIHDNVSIGHGAVVHGCTVEEGAMIAIHAT
                     VLTGAVVGAGSIVGAGAMVGEKKTIPPRSLAVGIPARVIRELTDEDVAMSAATARNYA
                     QLSKEHIAESGAATDGLDRE"
     gene            complement(3266..>3797)
                     /locus_tag="VHS06_06025"
     CDS             complement(3266..>3797)
                     /locus_tag="VHS06_06025"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX2987713.1"
                     /translation="RPLLFRTRSSFRDGGTASATSLLRCQMWEVELLGTGARRTIVGL
                     SLLGAADSIYMLSYHLGLIGSLVCPFFGEGCNRVGRSSHARHFGVPNAAVGAAGYGAM
                     AVLAAMSGRQEGRRWSAPLLAATSLSAFGASVFLTWEQPFRVGAWCFWCLSSAAINLA
                     ILPLALRGAARKGDRL"
BASE COUNT          754 a         1160 c         1233 g          650 t
ORIGIN      
        1 tcgcatcgat caagaagacg gggcggctgg taaccgtgga ggagagccgc gtgtgcggag
       61 gctggggcgc tgaacttgcg gcgcgggtgg ccaccgacgg cttcgacagc ctggacgcac
      121 caatccagag gatcggtgct ccggacgtgc cgatccccag tagccccgcg ctggagaagc
      181 tatacgtgcc atccgaggag cagatcgtag ccgcagtgag gaagaccctg cagtagcagg
      241 gggtatccaa ttgaagtagg ggtggtacgt gcgccgcccc tgcttcagtg gcaatacatc
      301 tcacaacggg tccagggcac gcgatgcgag cgagcggagc atcgcggcct ggcggggcgc
      361 aacgctacgc ccgctcgcat tgcggggtgt ccccaaagtt acggtctctt tctactacct
      421 acctagcatt cgttctgagg ggtgcagcag atgacgagga gcgggatcga tgcctttctg
      481 aagcccaggt cgatcgcggt attgggggct tccgcccacg agggaaagat cggcaacata
      541 gttgtgagac agcttgccgg caagggtttc acgctctacc cggttaatcc caagggggga
      601 agcgtggagg gcatcccgat ccttcggagt ctgcgggagg ctccggaggc cccggaactg
      661 gcggtcctca cactgcctgc cgaggcggcc gtcccggctg cagctgagtg cgtcgagcgc
      721 ggagtccgtg gagtgatagc catagccgga ggattcggcg aaacagggga ggaagggcgg
      781 aagttagagg cacaactcgc ggatgtgatc aagggcagcc atactcgcct gctcggtccc
      841 aacaccctgg gggttctctc ccccggaacg ggtctggata cggtgttcct ctcgcccgaa
      901 cggttccagc gccccggggc cggtgccctg gcactgattt cccagagcgg gtccgcggtg
      961 ctaggtgaac tggacaacca ggcgctgcac ggcataacga tatcggcctt cgtggggctg
     1021 ggcaaccgac tggacgtaga cgaggaggag tggatccgtt acttcgccga cgatccggcc
     1081 tccacagtga tcgggctcta cctggaaagc ttctccgacg cgacacagtt gctggatacc
     1141 tgcgagcgcg tggttgccac caagcccata gtcctcctga aggcaggctg ctcggagtct
     1201 ggagcccggg cggttcaact ccataccgga tcgctggccg gatcggacca ggtcaccgac
     1261 ggcgcgctcc gtcaggcagg gatcctgagg gtgcgagact gtgaggaact gctggatgta
     1321 ggccgtaccc tggcgtacgc ccgtccgctg gagggtcgca acgttgccgt actcaccaac
     1381 ggaggcggct ggggcatact tgcagccgac tgcatcgagt cgaaggagtg gggcgccggg
     1441 cttcggctgg caaggctgac cccagagaca acggggcgac tcggaaaggt caccctgccc
     1501 tatgcagcac tgagaaaccc ggtagacgtg actgccagcg ccgacaacaa gatgtttgcc
     1561 gatgcgctgg aaatcctgca ggacgccccc gaggtagatg ctgtgctgtg cacctttggg
     1621 ttccaaccgc cggccatcga cgagaggctg acggatgttc tgtgccacta cggcaagagt
     1681 ggcagaaagc cgttggtcgt gagcctgatg ggctcggagt cggccatgca cagtcttcgc
     1741 cggctggaag caacaggtgt cccggcatat ccctccacca agagggcagt gcgcgccatc
     1801 gccgccctcg ctcaacgagg tgagtacctg cgccgccgcc gcgatggaac gcaaactggc
     1861 attgcagcca tacgttctcg tggtccggag atcgacgacc agttccggcc tgggactccc
     1921 ctggccgagg atgaggtaaa ggcactgctg cgagcaaggg gaatagcgac accgaacggc
     1981 gtcgtcctgg ataggggact ccttcccgag accctctatt tggcgtaccc cctggcgctc
     2041 aaggttcgct ccgcggcaat cctgcacaag accgaccgag gcggcgtgat gctcgacatc
     2101 aaggatcgac cgcagttgga gacggccgtg gaggagatgc gatctcgctt cccgggcgaa
     2161 gacctcctgg cagaggagat ggagccgaag ggcgtggagc tgatcgtcgg gctgatatcg
     2221 gatcccacct ttgggctgtc gatcatgtgc gggatcggag gggtactggc cgagctgtac
     2281 agggacgtta ccttccgccg tctccccata gaccggatcg atgcagacga gatgataggc
     2341 gagctgaacg cccacgccat tctggaaggc ttccgcggga tcaaggcaag ccgcgaagcc
     2401 gtggtagagc tactgctcag ggtgtccgca cttggcgtgg agctggggga gggcgtcgac
     2461 cagctagatc tgaacccggt gatagtgcac gaggacggcg cggtggtggt cgacgccaag
     2521 ctgctgtgga agtggtgagg gcggttgccc tcaccctcta ctactcgcgg tccaggccgt
     2581 cggtggcggc gccgctctct gcaatatgct ccttggagag ttgggcatag tttcgcgccg
     2641 tcgccgccga catcgccaca tcctcgtccg tcaactctcg aatgacccgc gcggggattc
     2701 ccacagccag ggaacggggt gggatcgtct tcttctcgcc caccatcgcg cctgccccga
     2761 cgatgctgcc cgcacctacc accgctccag tcagaaccgt ggcgtggatg gcgatcatcg
     2821 cgccctcctc gacggtgcag ccgtgaacca ccgcgccgtg cccgatggac acgttgtcgt
     2881 ggatcacaca gggaacaccc cgcccgacgt gcacggtgga gttgtcctga acgttgacat
     2941 tctgcccgat ggtcacgctg gccacatcgc cccggatgac ggtgttgtac caaacgctgc
     3001 tgcccgcccc aatagtcacg tcgccgatta tctgggcacc ctccgcgatg aagacgccct
     3061 cggcgatctt cggcatcaca cccctgtaag ggatcaaagc catctaagta gcctcctatc
     3121 ggcagaaccc tgcctagggc gattctaagt cgaagccgca aaaccggtca atgagcttat
     3181 tgtcgataac cctgtggata tttcgacggt aatgtggata aaaaccaagc ggacctcagt
     3241 ctggcagagg cgctagatgc ccgatctata gccggtcacc tttcctcgcc gctcctctca
     3301 gcgccaacgg caggattgcc agattgatcg ccgcggagct caggcaccaa aagcaccaag
     3361 cccccacgcg gaacggctgt tcccaggtca gaaacacact ggcgccgaag gcagaaagag
     3421 aggtggcagc cagcagaggc gcactccagc gacggccttc ctgtctcccg gacatcgcgg
     3481 ccagaaccgc catggcacca tagccggctg cgcctaccgc tgcattggga accccaaagt
     3541 gtcgagcatg agacgacctc cccaccctgt tgcatccctc tccgaagaaa gggcagacca
     3601 gggagcctat caggcccagg tggtaggaga gcatgtagat ggagtcggct gcgcccagaa
     3661 gggatagacc gacaatggtt cggcgagcac cagtgcccaa caactcaacc tcccacattt
     3721 ggcaccgtag gaggctggta gcagaagccg tgcctccatc gcggaaggag gaacgtgttc
     3781 taaataggag gggccga
//
LOCUS       DATALS010000272         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_209827, whole genome shotgun sequence.
ACCESSION   DATALS010000272 DATALS010000000
VERSION     DATALS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209827"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VHS06_06030"
     CDS             <1..897
                     /locus_tag="VHS06_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2987714.1"
                     /translation="TVEPGKGINPVIEDGVIHSDGTTILGADCKASIAAILESIRVIL
                     DEKTPHGDVEVLVSYGEEKGLLGAKTMDHTRLRAKYGFVADSSNRPGTIILSAPSQDS
                     MSVVFHGRCAHAGLEPEKGIDALRAAALAITRMPLGRIDDETTANIGLIRGGLGRNSV
                     PDRVALEGEARSRDNEKLDRQTRAMVEAMESAAADIGATVDIDISRQYESYRFAEDAP
                     IVQMAKSAAEAAGLRPRFGLSGGGADANVFNARGVTSLVLGTGMSQPHSVEESISIED
                     LLGLTRWMIELIRIAATMQVSP"
     gene            1057..2223
                     /locus_tag="VHS06_06035"
     CDS             1057..2223
                     /locus_tag="VHS06_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2987715.1"
                     /translation="MHGWIGQILRVDLTAGSCRVEPLDKGIAGDYLGGRGLGLRLLAD
                     EVPWNADPLGPENRMVFAAGPLTGTSAPAGGRFSLTTLSPLTGTIFDANSGGRWGVAL
                     KAAGFDAIVVQGVSERPVVLEVDIDGARLVGAEQLWGAGVAKTTATLGVGGRSVACIG
                     PAGERQVRLASIMNDGTRALARGGVGAVMGSRRLKAIVLSGNGKGSVADPDRFDFYRY
                     ESEKLLKASPLTAHGLPEFGTAVLVNVLNEAHALPTRNYQECRFEGAEAISGEALRER
                     FVTGKRGCWSCSIACTRRIEVDGVKGEGPEYETIWAMGADCGIDDLKVIVQANALCNE
                     LGIDTISMGATIACAMELTQRGKLPEGPTSARRTSPAWGGSSRDCRVRTGRKRA"
     gene            complement(2237..3157)
                     /locus_tag="VHS06_06040"
                     /pseudo
     CDS             complement(2237..3157)
                     /locus_tag="VHS06_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
BASE COUNT          569 a          967 c         1063 g          561 t
ORIGIN      
        1 accgtcgaac cggggaaagg gatcaaccct gtgatcgagg acggcgtgat ccacagtgac
       61 ggcaccacta tcctcggagc cgactgcaag gcgagcatcg cggccatcct ggagtccatc
      121 cgtgtgatcc tggatgagaa gacccctcat ggcgacgtcg aggtgctggt cagctatggg
      181 gaggagaagg ggttgctggg cgccaagaca atggatcaca cacggctccg ggcgaagtac
      241 ggctttgttg ccgacagctc caaccggccg ggcaccatca tcctctccgc gccgtctcag
      301 gacagcatgt cggtggtgtt tcacggcagg tgcgcccacg ctgggttgga gccggagaag
      361 ggaatcgacg ccctccgagc cgctgccttg gcgataaccc gaatgccgct gggaaggata
      421 gatgacgaga ctactgccaa catcgggctg atcaggggtg ggctggggcg gaactcggtg
      481 cccgaccgtg tggcgttgga gggcgaggct cgaagtcggg acaacgagaa gttggatcga
      541 cagacccgtg cgatggtgga ggcgatggag tcggcagccg ccgacattgg ggccaccgtg
      601 gacatcgata tatcgcggca gtacgagagc tatcggtttg ccgaggatgc ccccatagtg
      661 cagatggcga agagtgccgc tgaagcggcc gggctaaggc cccgcttcgg cctctctgga
      721 ggcggcgcag atgccaacgt cttcaatgct cggggggtga cctcgctggt gctgggtacc
      781 ggaatgtcgc agccccacag tgtggaagag agcatctcca ttgaggacct gcttgggctg
      841 acccgatgga tgattgagtt gatccgcata gccgccacta tgcaagtcag cccctaatcg
      901 gggaaaatgg gccgcagcaa catgaggcgg cgcaccccaa cactctgacc cacttccttt
      961 gtgtagatgg gatcgacccg atctttgcgc cctgtagcgc ggcgagggga ttccgtcgtt
     1021 ctatagcggt gggcgcgcgg ctggaaagga atggaaatgc atggctggat cgggcagatt
     1081 cttcgcgtcg atctgaccgc gggaagctgt cgcgttgagc cgttagacaa agggatcgcc
     1141 ggggactatc tggggggacg cgggcttggc ctgcggctcc tggccgacga ggtgccctgg
     1201 aacgccgatc ccctgggacc ggagaatcga atggtcttcg ccgcgggccc actgaccggg
     1261 acgtccgcac cggccggcgg aaggttctca ctcacgaccc tgtcgcccct caccgggacc
     1321 atcttcgacg ccaactctgg agggcgctgg ggggttgcgc tgaaggcggc gggtttcgac
     1381 gccatcgtcg tgcagggggt ttcggagcgg cccgtcgtgc tggaggtaga catcgacggg
     1441 gcccgtttgg ttggcgccga gcagttgtgg ggggccgggg tagctaagac cacggcgacg
     1501 ttgggagtag gcggccggag cgtagcctgc atcgggcctg ccggagagcg gcaggtacgg
     1561 ttggcctcga tcatgaacga cggcacgagg gcgctggcgc gcgggggcgt cggggcagtg
     1621 atgggctcca ggcggctgaa ggcaatcgtg ctttccggca atggaaaggg ttctgtcgcc
     1681 gatccggacc gcttcgactt ctatcgctac gagtcggaga agttgctgaa ggcgagcccc
     1741 ttgacggcgc acggcttgcc cgaatttggc accgcggtgc tggtgaacgt cctgaacgag
     1801 gcgcacgcgc tgccaactcg caactaccag gagtgtcgct tcgagggcgc ggaggccata
     1861 tccggcgagg cgctgaggga gcgcttcgtg acgggcaagc gtgggtgctg gagttgttcc
     1921 atcgcctgca ctcggcgcat cgaggtggat ggggtcaagg gagagggccc tgagtacgag
     1981 acgatctggg cgatgggtgc cgactgcggc atcgacgatc tgaaagtgat cgttcaggcc
     2041 aatgccctct gcaacgaatt gggcatcgat accatctcca tgggggccac gatcgcgtgt
     2101 gcgatggagt tgacgcagcg gggaaagcta cctgaaggac cgacttcagc tcggcgcact
     2161 tccccggcgt ggggaggatc ttcccgggat tgcagagtcc ggacgggtcg aaagcgtgct
     2221 tgagtcggct catcgcctcc agatcgcgct ccgagaatag ctccgacatc aattccttct
     2281 tctccacgcc gataccgtgc tcccctgaaa gcacgccccc cgcggctata cagatccgca
     2341 tgatttcctc tccggccgcc ctcgcccggt ccacctcacc gggcttctcc ccatcgaaga
     2401 gcaccagggg gtggaggttg ccatcgccgg cgtggaagac attcgcgatc ggaaggccgt
     2461 gcttcttgga gacgtcacct atggctacga gcacccccgg cagtttcgtg cgagggacga
     2521 ccccgtccac cacgtagtac tcagggctca accgccccat cgcgccgaaa gcgcccttac
     2581 ggcccgccca cagtaggtca cgctcctcag cgctttgagc tacccgcacc accctggcgt
     2641 tgtgtgctac cgcgatctcg cggattgcag cggcctgctc cgccagtccg tcccgaagtc
     2701 catccacctc cacgagcagc acggccccgg catcctcggg atatcccgcg tggtaggcag
     2761 cctccacggc cttgatggcc aactggtcca tcatctctat ggccgcgggc actatgccgc
     2821 ttccgattac gtctgttacc gtggcactgg cgtcgttgac gtcgtcgaag accgccagca
     2881 gagtctttac tgcctcggga agcggcacta tccgcactac cactttggtg gcgatgccca
     2941 gcgtgccctc acctccaacg aagaggcctg tgaggtcgta gcccggaaga tccaaggcgc
     3001 gcccaccgaa ccacacgacc tcaccgtccg gcatcacaac ctcgaccccc aggacgtggt
     3061 tggtcgtcac cccatacagg agggtgtggg gacccccggc gttctcgccg acgtttccac
     3121 cgatggtgca cgccttctgg ctggaggggt ccggcacgta
//
LOCUS       DATALS010000273         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_251599, whole genome shotgun sequence.
ACCESSION   DATALS010000273 DATALS010000000
VERSION     DATALS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251599"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VHS06_06045"
     CDS             <1..790
                     /locus_tag="VHS06_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2987716.1"
                     /translation="DLRFYEGPPATLSLLALHIAPEERALSRDERLTALLAQLAQVVP
                     DLEFGDLLPNRVVPSPVQRSEETLPPAAMQTAIAQPQCPTEEPLQSAVDGLLFQTMEL
                     ARNGHSHKALRALEELLHNYPTNGPAWKLREEIRLLERREKRRQKDPRNPQTILEVGF
                     SYLILERNRDAAEALRSAIRLDPKTHLSHLLLGIALHREGERDGAREAYLQAARLQPE
                     ADSVCNDLLRALERGEPPMPLTESNRASRPRPQPRTTFQWARAV"
     gene            complement(963..1937)
                     /locus_tag="VHS06_06050"
     CDS             complement(963..1937)
                     /locus_tag="VHS06_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX2987717.1"
                     /translation="MPLRALLPIFAALTLLSAACQSAPAADKGGAPTAAPAAPTAASV
                     APTKAPAAPTVAPTAAPTAAVNPFLLMSSTIGPIDAGIVGALEDAYTKKTGVKIRHVG
                     AGTGATLEMAKNGQFDLVLVHARALEDKFIADGFGVDRRDLMYNDFVILGQASDPAGI
                     KGEKDATVALKKIAAAKALCVTRGDNSGTDVKEKELWAKAGIKPAGDWYVVYEKGSTG
                     NGPTTKYADQRQAYVLMDRATIITLRKDIKLEILVEKGEDLLNYISLIKVNPAKFPKV
                     NAAGANQYVDWLVSDEAQQLIKNFGVAQYGEPLFFPNSAEWRKSHPAN"
     gene            complement(2218..2616)
                     /locus_tag="VHS06_06055"
     CDS             complement(2218..2616)
                     /locus_tag="VHS06_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2987718.1"
                     /translation="MLTGFGHIGIGVKDIEKSVAALCKLLEIPVPPIKEVPEKKAKVA
                     LVDLWGIGLEFIQDDSEDGMLGRFNREKGNGIHHFCLLTDDIEGDIKWMMNRGVKMMH
                     ETPRVGLRDKRVAFISPDSNEGVPIELSEP"
BASE COUNT          500 a          918 c          819 g          508 t
ORIGIN      
        1 cgatctgcgt ttctacgagg gccctcccgc gacgttatcc ctgctcgcgc tccacatcgc
       61 gcctgaagag cgggcgctct cacgcgatga gcggctgacc gccctcctgg ctcaactcgc
      121 gcaggtcgtc cccgacctcg agttcggcga cctgctgcca aatcgggttg tcccttcgcc
      181 cgtgcagagg agcgaagaga cgctaccacc cgccgccatg caaaccgcga ttgcccaacc
      241 tcaatgcccg accgaagaac ccctgcaatc cgccgtcgat ggcctccttt tccagacgat
      301 ggagctagca cgcaacgggc actcccacaa agcgctacgc gcgctggaag aactgttgca
      361 caactacccc accaacggcc cggcctggaa gctgagggaa gagatccggc tgcttgaacg
      421 gcgtgaaaag aggcgacaga aagaccccag gaacccccag accatcctgg aagtcgggtt
      481 ctcctacctt atcttagagc gcaaccgcga tgccgcggag gccctcaggt ccgcgattcg
      541 actggatccg aaaacccacc tctcccacct gctgctgggc atcgcgctcc accgggaagg
      601 tgagcgtgat ggcgccaggg aagcctacct gcaagcggct cgactccaac cggaagcgga
      661 cagcgtatgc aatgacctgc tgcgggccct ggaaaggggc gaaccgccga tgccgctgac
      721 cgagtccaac cgggcgagcc gccctcgtcc ccaaccacga acgacgttcc aatgggctcg
      781 agcggtctaa ccctccggcg cggtggtctt ctcctgtggg gcagaggctt cgcgccctgc
      841 cgccccgtcg ccgttgccgc gcgattgcgg cggggcggag ccgtgacgag gcgctcgcta
      901 ccgtcacgcg ccctacaggt catccctact ttgttcgaac cgtgctcact tatgccaagt
      961 ggtcagttgg ccggatggct ctttcgccac tcggcggagt tggggaagaa gagcggctcg
     1021 ccgtactggg ccacgccgaa gttcttgatc aactgctgcg cctcatcgga caccagccag
     1081 tcgacgtact ggttggcgcc agcggcgttc accttgggga acttggcggg gttcaccttg
     1141 atcagcgaga tgtagttcag cagatcctcg cccttctcca ccaggatctc cagcttgata
     1201 tccttcctca aggtgatgat ggttgctcga tccatcagca cgtaggcctg gcgctggtcg
     1261 gcatacttgg tggtggggcc gttgcccgta gagcccttct cgtacaccac gtaccagtcg
     1321 cccgcgggct tgatccccgc cttggcccac aactccttct ccttgacgtc ggtgccggag
     1381 ttgtcgccgc gggtcacgca cagcgccttg gccgcggcga tcttcttcag ggctacagtc
     1441 gcatccttct cacccttgat ccccgcggga tcggaagcct ggccgaggat gacgaagtcg
     1501 ttgtacatca ggtcgcgccg gtccaccccg aaaccgtcgg caatgaactt gtcctcaagg
     1561 gcgcgggcgt gcaccaggac gaggtcgaac tggccgttct tggccatctc cagggtggcg
     1621 ccggtccccg cgcccacgtg ccggatcttg accccggtct tcttggtgta ggcatcctcc
     1681 agcgcgccga cgatgccggc gtcgatggga ccgatggtgc tggacatcag caggaagggg
     1741 ttaacggcgg ctgtgggtgc cgcagtgggc gcaacggtgg gggcagccgg cgcctttgtc
     1801 ggtgcgacgg aagcggcggt gggagctgca ggggcggcag ttggagcacc gcccttgtcg
     1861 gcagctggtg cagattgaca ggcagcggag agcagggtca gcgcggcaaa gatcgggagg
     1921 agcgcacgta agggcatggt aaacctccac ctcttggccc ccgccacctc ggatccggtc
     1981 gcccgcctcc aggtagcgcg aaggccgact agcacgcaaa agcggcagtc gctgctgccg
     2041 ctctcgcgct ggctggttac accggaggga gagaactctg cagaaaaccc tgccgtcctt
     2101 catgccggcc ctcgccccaa gggccagcat gaagagcaac caggtctaag aggagttgag
     2161 tcttcccttc gctgtcgggg ttatcatact acatgtagta caattgtcaa gagactctca
     2221 cggctcggac aactcgatcg gcaccccttc gttggagtcc gggctgatga aggcgaccct
     2281 cttgtcgcgg aggccgaccc gcggcgtctc gtgcatcatc ttcacaccgc gattcatcat
     2341 ccactttatg tcaccctcga tgtcatctgt cagcaagcag aagtggtgga tgccgttccc
     2401 cttctctcgg ttgaaccggc ccagcatccc atcctcgctg tcatcctgga tgaactccaa
     2461 gccgatgccc cacagatcca ccaacgccac cttcgccttc ttctcgggca cctccttgat
     2521 gggcggaacg gggatctcca gcagtttgca cagcgccgcc acgctcttct cgatatcctt
     2581 gacgccaatg ccgatgtggc cgaaaccggt tagcatgtga ctctgccccc actcacgatt
     2641 ctgagaatga ttgtggtccg aggtcggtcg cggagcccat cagagctgcc ccaaccgcac
     2701 cactctcatg atgccgaaaa ggattgtaga cgcaagagtg cgacc
//
LOCUS       DATALS010000274         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_28064,
            whole genome shotgun sequence.
ACCESSION   DATALS010000274 DATALS010000000
VERSION     DATALS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28064"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VHS06_06060"
     CDS             <1..657
                     /locus_tag="VHS06_06060"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2987719.1"
                     /translation="PDSAQPFGLNPLVLESVQKDDRLVGGLWVSLLPKDKERVIESLS
                     HAGSPRIKILKMTCLLGGTWDVNKWDEATQEIGDMIVDTAEKHNYVIHIHTSPGGSSD
                     ISAAFPFAEKYGKRVKIHLVHMGGGVSGHIKMVPRFLQMVKEGYQIYTDCSWSVGFGP
                     RFLLTEIERTGIGADRVLFSSDIPWSDFMGEYWKIEGAPVSEELKENIFWNNAEKLYG
                     "
     gene            792..1277
                     /locus_tag="VHS06_06065"
     CDS             792..1277
                     /locus_tag="VHS06_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572/Sll0783 family nitrogen starvation
                     response protein"
                     /protein_id="HEX2987720.1"
                     /translation="MAMVEVPRLGEGDEIVNLQEKLFPDYKANPGDKALIMMHTVPFE
                     GSVGLVNMLTATRIRRKGFDTSFVLYGPGVLMAAAGRGFPKVGMEGFPGNLSVNKQLT
                     TIMNEGGKVYACRFAMAALYGMRETDLMEGVRAFSPLDVLDITIENWRAGALILSTWT
                     V"
     gene            1603..2682
                     /locus_tag="VHS06_06070"
     CDS             1603..2682
                     /locus_tag="VHS06_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003351901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0568 family radical SAM protein"
                     /protein_id="HEX2987721.1"
                     /translation="MADQAIVTELQSLGARIEVPIKERGRRGGAGPSDGKPFVIGEVA
                     TTVPTLGEFAEKSPYAVVESEDGYQLTRNGRRLMPVRFPPTPCFYDLTTADGIPYRQI
                     ALMHGVDTLATTVFQKCVHWHRPGERCQFCAIELSLERGATIVMKTPEQLAEVAEAAG
                     RLDGASHFILTTGTINLKDKGVRYLARCAQAIKASTSIGIQAQFEPPDDLEDMVLLRD
                     AGVESVGLHLESFDQSVRERVAPGKAKIGIERYFEAYRRAVELFGRNQVSSYIIVGLG
                     EGPESIVEGCRSLAKIGVYPFVVPLRPIIGTLMQDARTPSPELMASIYTQVAAINREQ
                     GLSWRNSKAGCERCGACSALPAYEG"
BASE COUNT          515 a          757 c          908 g          517 t
ORIGIN      
        1 ccggattcgg cccagccctt cggcctgaat ccccttgtgc tggagagcgt gcagaaagac
       61 gatcgcctgg ttggaggttt gtgggtatcc ctcctgccaa aggacaagga gcgggtgatc
      121 gagagcctca gccacgcggg ctccccgcgc atcaagatcc tcaagatgac ctgcctattg
      181 ggtggcacct gggatgtcaa caagtgggac gaggcgaccc aggaaatcgg cgacatgatt
      241 gtcgacaccg ccgagaagca caactacgtg atccacattc acaccagccc gggcggcagt
      301 tccgacatct ccgcggcctt ccccttcgcg gagaagtacg gcaagcgagt caagatccac
      361 ctggtccaca tgggtggcgg cgtcagcggg cacatcaaga tggtgcctcg cttcctccag
      421 atggtgaagg agggctacca gatctacacc gactgctcct ggtcggtggg cttcggcccg
      481 cgcttcctcc tcaccgagat cgagcgcacc ggcatcggtg cggatcgtgt cctcttctct
      541 tccgacatcc cgtggagcga tttcatgggc gagtactgga agatcgaggg cgcgcctgtc
      601 tccgaggagt tgaaggagaa catcttctgg aacaacgcgg agaagctgta cggatagacc
      661 gaggtttcgg cggggtgatc gcagattgcg ccggtgggct cccggggggt gggccgcggc
      721 tgcggtagcg ttggtttctg gtgtgtgcac cagacgtttg tgcagcagaa taggtaagga
      781 gggaactggc gatggcaatg gtggaagtgc ctcgattggg cgaaggggac gagatcgtaa
      841 accttcagga gaagctgttc ccagactaca aggcaaaccc gggtgacaag gccctgatca
      901 tgatgcacac cgttcccttc gaagggtcgg ttgggctggt gaacatgctc acggcgaccc
      961 ggatccgccg caaggggttc gacaccagct tcgttctgta tggccccggc gtcctgatgg
     1021 cagcggccgg ccgcggcttc cccaaggtcg ggatggaggg gttccccggc aacctttccg
     1081 tcaacaagca gctcaccacc atcatgaacg agggtgggaa ggtctacgct tgccgtttcg
     1141 ccatggccgc gctgtacggc atgcgtgaga ccgacctgat ggagggcgtg cgcgccttca
     1201 gcccgctgga tgttctcgac ataaccatcg agaactggag agcgggtgcc ttgatcctga
     1261 gcacctggac ggtctagcaa cgattaccgg ctaagcagtc ctgggacacg gtgaactcga
     1321 caccaggagt cgaggtcacg ccgggctcct ataggtcgag ggccaccgtg cctgaggact
     1381 ctatcgtggt tcgagccggg tccggcggcg gatggttgaa ggcagtcagt gaccaatcac
     1441 gtcggcactc tcacccggga ggccgaggtg gttgtgcatc ggcagtcgtc tacctctgca
     1501 agggctagct gccggtcccg gcgccatttt aaccataggc tgatgatgct cgtggtcggc
     1561 gctttctacc tgttcaaggc gaccccaagg aggggatgag aaatggcgga tcaggcaatc
     1621 gtgaccgagc ttcagagtct gggtgctcgt atagaggtgc cgatcaagga gcgtggccgg
     1681 cgtggcgggg cgggcccttc ggatgggaag ccgttcgtga taggggaagt cgcgactacg
     1741 gtgccgactc tgggtgagtt cgctgagaaa tcgccctacg ccgtcgtgga gagcgaggac
     1801 ggctatcagt tgacgcggaa cggccggcgg ttgatgccgg tccggtttcc gccgacgcct
     1861 tgtttctacg acctcaccac cgcggacggc attccgtacc ggcagatcgc gctgatgcat
     1921 ggcgtggaca ccctggcgac cacggttttc cagaagtgcg ttcactggca ccgtcctggc
     1981 gagcgctgtc agttctgtgc tatcgagctg tcgttggagc gtggggccac catagtgatg
     2041 aagaccccgg aacagttggc cgaggttgcc gaggcagcgg gccggctgga cggcgcgagc
     2101 catttcatcc tcaccaccgg caccatcaac ctgaaagata agggcgtgcg ctatctggct
     2161 cgctgcgctc aggcaatcaa agcgtccacc agcatcggca ttcaggcgca gttcgaaccc
     2221 cccgatgatc tggaagacat ggtgctgctg cgcgatgccg gcgtggagag cgtggggctg
     2281 cacctcgaaa gcttcgacca gtcggtgcgc gagcgcgtgg cgccgggcaa ggcgaagata
     2341 ggcatagagc gttacttcga ggcttaccgg cgggccgttg aacttttcgg tcgaaaccag
     2401 gtttccagct acataatcgt cgggcttggg gagggccctg agtctatcgt cgagggttgc
     2461 cggagtctgg cgaagatcgg tgtataccct ttcgtcgtgc cgttgcggcc gattatcggc
     2521 accctgatgc aggatgcgag aacgccttcg cccgaactga tggcatctat ctacactcag
     2581 gtagcggcta tcaaccgaga acagggattg agttggcgca acagcaaagc gggatgtgag
     2641 cgctgtgggg cttgctctgc gttgccggcc tacgagggct gagggaggta gagatgg
//
LOCUS       DATALS010000275         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_161762, whole genome shotgun sequence.
ACCESSION   DATALS010000275 DATALS010000000
VERSION     DATALS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161762"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VHS06_06075"
     CDS             complement(<1..620)
                     /locus_tag="VHS06_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987722.1"
                     /translation="MAGRSGARWRSVGKAFAIAAVLAAPVVLGSCKSLSEMLRGTQQP
                     QQPATTPAPTPAPAPATPTPAAPPPTQAAPATTSGPVAAAVSQEQAQALVRDWFGALQ
                     AGDYQRAQSLTTGSANQQTRTLTDTIQREAAQRGVQASIAVNRLDLSPAPQPAQGQAV
                     RSAFDISVNVQVGPVTVPARQFQGSATFVVQQTDQGLKITDIRDTQG"
     gene            complement(822..>1503)
                     /locus_tag="VHS06_06080"
     CDS             complement(822..>1503)
                     /locus_tag="VHS06_06080"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX2987723.1"
                     /translation="YRDMGYLPETLVNYLALLGWAFDATTEIMSREELIRNFSIEKIN
                     KTGAVFDINKLDWMNGYYIRQLSLPDLASRLMPFMEKAGLATEIDKAYFERVLPLVQE
                     RIKKLSEIVEFTDLFFREKLEYDSSLLVAKGLTAESSLAALRTATERLEGVTAWDHPT
                     LEEVIRPLAAELELKTGQLFGILRIASTGRTAAPPLFETMEVLGRDRVMERLAGAEQR
                     LAELAKAS"
BASE COUNT          226 a          477 c          476 g          324 t
ORIGIN      
        1 ccctgcgtgt ccctgatgtc ggtgatcttg agcccctggt cggtttgctg cacgacgaag
       61 gtggcgctcc cctggaactg gcgcgcgggc acggttaccg gccctacctg cacgttcacg
      121 ctgatgtcga aggccgatct cacagcttgc ccctgtgcag gttgcggcgc tggggataga
      181 tccagcctat tcactgcgat gctcgcctgc actccccgct gagccgcctc ccgctggatg
      241 gtgtcagtga gcgtcctggt ttgctggttg gcgcttccgg ttgtcaggct ctgcgctcgc
      301 tgatagtcgc cggcctgcag tgctccgaac caatcgcgca ccagggcctg agcctgctcc
      361 tggctgacag cggcggccac agggccagaa gtggtcgcgg gtgccgcttg tgtagggggt
      421 ggtgccgccg gggtgggagt cgccggtgct ggtgccggtg tcggcgccgg cgtagtggcg
      481 ggttgctgcg gttgctgggt tcctcgtagc atctcactca gggatttgca gcttcccagc
      541 accaccggcg ccgccaggac cgcggctatg gcaaacgctt tgccaacact tcgccatctc
      601 gccccacttc ttcctgccac gacagcctcc acgcaactct ggggatcgtt ccctgtctct
      661 gggcgctcga tagagcacat tctacccgct cagatcgagg gactgtggca aggtgcggac
      721 caagagggag tggcttggcg atgcgcgcca aatggagtag aggcttcagc cggaacattc
      781 ttcttccggc tgaagcctct actcgtggtg actatgtgag gctagctcgc ttttgccaac
      841 tcggccagcc gctgctcggc gccggccagc ctttccatga cgcgatcgcg acccagcacc
      901 tccatagtct cgaacaacgg cggtgcggcg gtgcgccctg tggaggcgat cctcaggatt
      961 ccgaagagtt ggccggtctt cagttccagt tccgcggcca gcggccggat cacctcttcc
     1021 agcgtcgggt gatcccatgc tgtgacgcct tccagccgtt ccgtggcagt gcgcaacgcg
     1081 gccaggctgg attccgcggt cagccccttg gcgaccagca ggctgctgtc gtactccaac
     1141 ttctccctga agaagaggtc ggtgaactcc acgatctcgc tgagcttctt gatccgctcc
     1201 tggaccagcg gcagtacccg ttcgaagtat gccttgtcga tctcggtggc cagtcctgcc
     1261 ttctccatga agggcatcag cctggaggcc aggtcgggaa ggctgagctg gcggatgtag
     1321 tagccgttca tccagtccag cttgttgatg tcgaagacgg ctccggtctt gtttatcttc
     1381 tcgatggaga agttgcggat caactcctcg cgcgacatga tctccgtggt ggcgtcgaag
     1441 gcccagccca gcagcgccag gtagttcacc agtgtctcgg gcaggtaacc catgtctcgg
     1501 tag
//
LOCUS       DATALS010000276         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_103225, whole genome shotgun sequence.
ACCESSION   DATALS010000276 DATALS010000000
VERSION     DATALS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103225"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2786)
                     /locus_tag="VHS06_06085"
     CDS             complement(<1..2786)
                     /locus_tag="VHS06_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX2987724.1"
                     /translation="MFLRREPTRNWKLILVPHTHWDREWYLPFQEFRAVLVEAVDSLL
                     DIMSANPEFRYFTLDGQTIILEDYLEVRPEREDELKRRIQEGRILVGPWYVMPDEFLV
                     SGESLIRNYLEGQRTAARFGSSMDVGYLPDTFGHIAQMPQLLRGLGMDTTVLWRGVGK
                     AIQKSEASWVAPDGSSVLLEYTPVGYANAALLPTASDPLMERLSHIREQLEPHATTNF
                     LLLMNGDDHMHPQRDLPAIIQEANRRLRDAELVLGTLPIFLDGMKAAAAASGVELETI
                     EGELRSCETAHLLAGVLSSRMEIKQRNFNCETLLERWAEPFTNFAALLPRDPAAEQPR
                     STAPALLRLAWRYLLKNQPHDSICGCSIDQVHQEMQTRYDSCEQIGNRVLDRAVEAIA
                     AATDSEAILGNREGEGAVTVFNSEEGPRTDFVEATAQLPGEPGEVALVAPDGSVVPYQ
                     VLRGQQVELASSTLRRSEIQGYLRLAGPGRDWPRWKLRILEKIVKTALRDRLPQLVVD
                     DMDVVPGTDPSTVDVAVEVKAGREHNYDALSTGLRQLSSLVDRGDAQLFRLRIRRRDQ
                     FHVGFVAPRVPAHGLKLFRFEVAAPPRHTPLHNHGEATIENESLSLQVSPEDGTARLI
                     DRETGAIFWGLNSFVDVGDAGDEYTYSPPTHDRIVEGPDAPPSIVLEESGPARQRVRI
                     EMELRLPKGLSEDRQSRAEETVLCRVVSRFSIYPGVPRVDVHTTVTNRASDHRLRVRF
                     PTQLDARFSHADGQFAVIHRPIAFPPPSEGWVEKPTTSNPQLNFVDLSDGESGLMIAN
                     RGLPEYEVEQMAKGPCLSVTLLRCIGWLSRDDLSTRTGAAGPLLPTPGAQMPGIHSFD
                     YSIIPHAGEWDQAASQAFWFARPLRSRWTGRHPGPIGQEASFLALSPSSLQLSAVKQA
                     DDGSDTLIVRLYNML"
BASE COUNT          545 a          899 c          930 g          605 t
ORIGIN      
        1 agcatgttgt acagtcgaac gatcagcgta tcgctgccgt cgtctgcctg cttgacggcg
       61 gagagctgca gggaggatgg cgacagcgcc aggaagctcg cttcctgtcc gatgggaccg
      121 gggtgtctgc ctgtccacct ggacctcagg ggtctggcaa accagaacgc ctgactggct
      181 gcctgatccc actcgcccgc gtgtggaatg atcgagtaat cgaagctgtg gatgccgggc
      241 atttgggcac caggcgtagg aagcaacggg ccggcggccc cggtgcgagt ggacaagtca
      301 tcccgtgaga gccatccgat gcaccggagc agggtaacac tcaggcaggg ccccttggcc
      361 atctgctcga cctcgtactc cggtagtcct cggttagcta tcatcaatcc ggactcccca
      421 tcggaaagat ccacgaagtt gagttggggg ttgctggtcg ttggcttctc gacccaccct
      481 tccgatggag gaggaaaggc tatgggcctg tggatcacgg cgaactggcc gtcggcatgg
      541 ctgaagcgag cgtccagttg agtcggaaag cggaccctga gccggtggtc ggaagcgcgg
      601 ttggttacgg tggtgtggac atccacccgc ggaacacccg ggtatatgga gaaccggctc
      661 accaccctgc agaggacagt ctcctctgcg cgcgattgtc ggtcctccga tagccccttc
      721 gggagccgta gctccatctc gatccgcact cgctgacgtg ccggcccact ttcctccagg
      781 acaatacttg ggggagcatc cggaccttct acgatccgat cgtgagtcgg cggggagtag
      841 gtgtattcgt ctcccgcatc tcctacatcg acgaagctgt tgagccccca gaagatggcc
      901 cccgtttccc ggtcgatcag cctggccgtc ccatcctcgg gactgacctg cagtgagaga
      961 gactcatttt cgatggttgc ttcgccatga ttgtggaggg gggtatgtcg tggaggggca
     1021 gcgacttcga atctgaacag cttcagtcca tgagccggga cccgtggggc aacaaaaccg
     1081 acatggaact ggtcgcgtct cctgatcctg agtcggaaca gctgtgcgtc gccgcggtcc
     1141 accaggctgg acagttgacg aagtccggtc gacagggcat cgtagttgtg ctctcgtccc
     1201 gccttcacct ccacggccac gtcgactgtg gatggatcgg ttcctgggac gacgtccatg
     1261 tcgtccacga cgagctgtgg cagccggtcc ctgagagccg tcttgacgat cttctctagt
     1321 attctcagct tccagcgggg ccaatcgcgc cctgggccgg cgagccgcaa gtatccctga
     1381 atctcggacc gtctcagagt ggaactggcc agttccacct gttggccgcg caatacctga
     1441 taggggacga cgctcccgtc cggggccacg agggcgactt ctccgggctc gcccgggagt
     1501 tgcgctgtgg cctccacgaa gtcggtcctt gggccttcct cagagttgaa tactgttact
     1561 gccccttctc cctcgcgatt cccaaggatg gcctcgctgt ccgtcgcggc ggcaatcgcc
     1621 tccaccgccc tgtccagcac ccggttcccg atttgctcgc aggagtcgta gcgagtctgc
     1681 atctcctggt gaacctggtc gatgctgcag ccacagatgg agtcatgcgg ctggttcttc
     1741 aacaggtacc tccaggccag tctcagcaat gctggggcgg tggagcgtgg ttgctccgct
     1801 gccggatctc gtggaagcag ggcggcgaag ttggtaaacg gctcggccca ccgctcaagc
     1861 agcgtctcgc agttgaagtt gcgctgcttt atctccatgc gtgaggagag cacccctgcc
     1921 agcaggtgag ccgtctcaca ggaccgcagt tcgccttcga tcgtctcgag ttcgacacca
     1981 gatgctgcag cagcggcctt catgccgtcg aggaagatgg gtagcgttcc cagtacgagt
     2041 tccgcgtccc tgagtctgcg attcgcctcc tggatgatgg ccggcaggtc acgctgcggg
     2101 tgcatgtgat cgtcaccgtt catcaggagc agaaagttgg tggttgcgtg cggctccagt
     2161 tgctccctga tgtgagacag ccgttccatc agagggtccg atgccgtcgg aagcagcgcc
     2221 gcgttggcat agcccactgg ggtgtattcc aggagaaccg aactgccgtc aggggcaacc
     2281 caggaagctt cgctcttctg gatagccttt cccacgccgc gccacagcac ggtcgtgtcc
     2341 attccgaggc ccctcagcag ttgaggcatc tgtgctatgt gcccgaaggt gtcggggagg
     2401 tagcccacat ccatggagga accaaatcgc gctgcggtcc gttggccctc gagatagttg
     2461 cggatcagtg attccccgct gacaaggaac tcgtccggca tgacgtacca cggaccgacg
     2521 aggatgcgtc cctcctggat ccgccgtttc agttcgtcct ctcgctcagg ccgcacctcc
     2581 agatagtcct cgaggatgat tgtctggcca tccaatgtga agtagcggaa ttcgggattg
     2641 gccgacatga tgtcgagcag actgtccaca gcctcgacca gcacagcacg gaactcctgg
     2701 aagggaaggt accattcccg atcccagtga gtgtggggga ctaggatcag tttccaattc
     2761 cgggtgggtt ctcgacggag gaacaaccgt gactcctctg gcagactgct cttcaacgcc
     2821 ggcctaccac ggcgcaacaa acatgccaac tgaggctttg gagaaggggc acgtcctttg
     2881 ccagggagaa gacgagacga gaagagtgga ggctgcaact tgtccgcgag gcgaggaagc
     2941 gacggcgcaa gacgattgtt ggttattccc tttgccccc
//
LOCUS       DATALS010000277         5422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_162098, whole genome shotgun sequence.
ACCESSION   DATALS010000277 DATALS010000000
VERSION     DATALS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162098"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VHS06_06090"
     CDS             complement(<1..1167)
                     /locus_tag="VHS06_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2987725.1"
                     /translation="MGQGLNRRGMVVSRFWLEGAILTFVAGFAVLTFLAITLYRDRPP
                     MPGQVLDPSGQLLFTRQDIFAGQGVFQKYGLMEYGSIFGHGAYLGPDFTAEYLHLQAE
                     EMSGPTGSGGLGAEGPTPLERVAAELHQNRYDPASDTLIFGSGQAGAFERLRAFYAAR
                     FNSPTGAGIVPPRYIQDPRELQQLTSYFAWSAWVATANRPGYGYSYTANWPPEILAGN
                     LPTSDAVVWSILSIVALLGGVGLVLFLFGRYDWLGWRGAELEGRRVHFFPPGEVALTP
                     GQRSTAWFFLVVALLFLVQTLNGGLIAHYRADPTGGFYGLNLSALLPYNLARTWHLQL
                     SIFWVATAYLGAGIFIAPLISGWEPRGQKVLSYLLLGALVVVVVGSLLGEAGSMH"
     gene            complement(1231..2001)
                     /locus_tag="VHS06_06095"
     CDS             complement(1231..2001)
                     /locus_tag="VHS06_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2987726.1"
                     /translation="MAEERPILRMEGVSYSYMGRYPALVGVDLSVARGERLVILGANG
                     CGKSTLLKIADGLIRPDAGRAETCGEDASAAVDDLAVAHRLHRQVGLVFQDADVQLFS
                     PTVWDDVAFGPLQMGWDDGEVRRRVESALELMGIEALARRAPYELSEGEKKRASIATV
                     LSLDPDLLLLDEPTANLDPRSKGVLVELIARLADEGKTVVTTTQELEIAPEVGRRALV
                     FGYSERRQVAEGPVLRVLDDTDLLVRANLIHPRLLWRR"
     gene            complement(2133..3002)
                     /gene="cbiQ"
                     /locus_tag="VHS06_06100"
     CDS             complement(2133..3002)
                     /gene="cbiQ"
                     /locus_tag="VHS06_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEX2987727.1"
                     /translation="MDQPLVRQSRRRGGGDFVARTVAGVSGALERALFSEEQARLPGL
                     LQGMDPRAKVVAALSLLLAVSLARHTTIVVVVCGLALLLAGVSRLSLPLFAKRVWLGI
                     PLFAGVVVLPSLFMLPGRPLLTIVDLQFLRLVVSDNGLAGALLLIARVGASVSVALLL
                     VFTTPWTELVRAFRVLRLPGSFVVVLGMTYRYLFLFLHSANSLFLSRASRTVGETSGA
                     EQRRWAAGAAGALMGRSLKMSGDVLVAMRARGFDGEVRSACLPALRDEDWLLVACSVA
                     LSAGLLLADMGIR"
     gene            complement(3010..4044)
                     /gene="cbiM"
                     /locus_tag="VHS06_06105"
     CDS             complement(3010..4044)
                     /gene="cbiM"
                     /locus_tag="VHS06_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HEX2987728.1"
                     /translation="MHIPDGYLSPATVAVAYGAAAPFWYQAGKRVKETLRGQSAPLVA
                     LFAAFSFTIQMFNIPVPGGTTAHAVGGTLMAVVLGPWAAVVGVSTALVIQALFFGDGG
                     ITAIGANCFNMGIALPLVGYALYRLLAGHSPSPRRRAVAAAVGGYIGINTAGLLAGLE
                     LGIQPLLWSEGGRALYNPYGLDVTLPAMMLVHLTLAGFAEAALTGLGVAFLLRSHPAL
                     LGEAAAGASGNGLGRAGWSLVSVLGAMVVLAPLGLLAPGGAEFEWGKSELEGMVGYVP
                     SGLARLADSWQLAPLRGYQAPGMGPDSSFLQQAPAYILSAVVGVACIFVLLFAARVAL
                     GRPASAARRD"
     gene            complement(4267..4737)
                     /locus_tag="VHS06_06110"
     CDS             complement(4267..4737)
                     /locus_tag="VHS06_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX2987729.1"
                     /translation="MCTSTDPGVGKRLATEARGQIVAALQGRGLRLTTQREAVCEAIF
                     GCSGHICAEHILESVTVHHPGLRMNKTTVYRALDLLLELGLVSEHKCGDGRAQYEPAS
                     HGPHSHMICRRCGRLLDMDESVASAIRRRLGEEHGFQVELESYPVFGLCPECRT"
     gene            5025..5300
                     /locus_tag="VHS06_06115"
     CDS             5025..5300
                     /locus_tag="VHS06_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987730.1"
                     /translation="MVRFEVVPRCNGCKSCEMACSFRATGCFWPPASAIHTRRTGSKG
                     AYRYEIGIDADECDLCENEAIALCQKACGPCILTGEVISRIRGEVAV"
BASE COUNT         1108 a         1871 c         1642 g          801 t
ORIGIN      
        1 gtgcatactg cctgcttccc ccagcaggct tcccaccacc acaaccacca acgcgcccag
       61 cagcaggtaa gacaggacct tttgccctct cggctcccat ccagagataa ggggtgcgat
      121 gaagatcccg gcgcccaggt aggcggtggc tacccaaaag atggagagct gcaggtgcca
      181 ggtgcgggcg aggttgtagg gaagcagcgc cgagaggttg agaccgtaga agccgccggt
      241 gggatcggcc cgatagtggg cgatcagtcc gccgttgagt gtctgaacca ggaaaaggag
      301 cgccacgacc aggaagaacc aggcggtaga tcgctggccc ggtgttaatg cgacctcgcc
      361 gggcgggaag aagtgcaccc gacgtccctc tagctcggcc cctcgccagc ccagccaatc
      421 atatcgcccg aagaggaaca ggacgagtcc gacgcccccc agcagcgcga ctatcgagag
      481 gatactccag actaccgcgt ccgaggtggg aagattgccg gccaggatct cgggaggcca
      541 gttggccgtg taggagtagc cgtagcccgg ccgattggcg gtagccaccc aggccgacca
      601 cgcgaagtag gaggtgagct gctggagctc tctggggtcc tggatgtagc gcggggggac
      661 aatgccggcc ccggtcgggg agttgaaacg agcggcgtag aaagctcgga gccgctcgaa
      721 agctccggcc tggccgctgc cgaagatcag cgtatccgag gccgggtcgt accgattctg
      781 gtgcaactcc gccgcgactc gctccagcgg cgtcggaccc tccgctccca ggccgcccga
      841 gccggtcggg ccgctcatct cttccgcctg gaggtgcagg tactctgccg tgaaatcggg
      901 gccaaggtag gcaccgtggc cgaagatgga gccgtactcc atcagaccgt acttctggaa
      961 gactccctgg ccggcgaata tatcctgcct ggtaaacagc agctgcccgg aaggatccag
     1021 gacttgcccc ggcatcggcg gtctgtcgcg gtagagggta atggccagaa aggtcaggac
     1081 cgcgaatccg gctacgaagg tgaggatcgc accctccagc cagaagcgcg agacgaccat
     1141 tcccctgcgg ttcagcccct gtcccattgc gcacacccca tgtaagctta cagggttggc
     1201 ctcaacagga gcctcaacga ggctgcccgg ctatcgcctc cagagcagcc tggggtgtat
     1261 caggttcgct ctgaccagca gatcggtgtc atcgaggacg cgaagaaccg ggccctccgc
     1321 cacctggcgc cgctcgctgt agccgaaaac gagggctcgg cgacccacct ccggagcgat
     1381 ctccaactcc tgtgtggtgg taaccaccgt cttcccctcg tcggccaaac gcgctatcaa
     1441 ctccacaagc acgcccttgc tccgggggtc caggttggcc gtgggctcat ccagcagaag
     1501 cagatctgga tccagcgaga gaaccgtggc gatggatgcc ctcttcttct ccccctcgga
     1561 cagctcgtag ggagcccttc gggccagcgc ctcgatcccc atcagttcga gcgccgactc
     1621 cacccggcgg cggacctcgc cgtcatccca tcccatttga agagggccga aggccacgtc
     1681 gtcccacaca gtgggggaga aaagctgcac gtccgcatcc tggaagacca gtccaacctg
     1741 gcgatggagc ctatgggcca cggcgaggtc atccacggcg gcggaagcat cctcaccgca
     1801 cgtctccgcc cgtccggcgt ccggacggat caggccgtcg gctatcttca acagtgtgga
     1861 cttgccgcag ccgttcgccc ccagtatcac cagccgctcc ccacgcgcca ccgacaggtc
     1921 cacaccgacc agcgcggggt atctgcccat gtaggagtag gacacgccct ccatcctcag
     1981 tatcggtcgc tcttccgcca cggctcaaag gatccttcca tacattatgt aaaccatatt
     2041 acgcaaacac acgctgccac agcgacactc agtggtgcat ccgccgagtc gctggcaggg
     2101 ctgcgggccg ctaatcggta gttcggcggc ctttacctga tccccatatc tgccaaaagc
     2161 aacccggccg acagcgccac gctgcaggcg accaacagcc aatcttcgtc ccgtaaggcc
     2221 ggcaaacatg cagaccgcac ctcgccgtcg aagcctcgcg cccgcattgc caccagcacg
     2281 tccccgctca tcttcagaga ccggcccatc aatgcacccg cggccccggc cgcccagcgc
     2341 cgctgctccg ccccagaggt ctccccaacg gtgcggctgg ccctcgagag gaacaggctg
     2401 ttcgccgagt gaaggaagag aaagaggtag cgataggtca tgccgagtac gacgacgaag
     2461 ctcccaggca gacgcagaac ccggaaggcc cgcaccaact cagtccaggg agtggtgaag
     2521 accagcagaa gcgccacgga caccgatgcg ccaacccgag cgatcagcaa cagggcgccg
     2581 gccaacccgt tatccgagac caccaggcgc agaaactgga gatccacaat ggtgagcaac
     2641 ggcctgccgg gcagcatgaa gagggacggc aacacaacca ctccggcgaa caggggaata
     2701 cccagccaca ccctcttggc gaagaggggc agcgataggc gcgaaacccc ggccagcagc
     2761 aacgccagcc cgcagaccac gaccacgatg gtcgtatggc gagccagact caccgccaac
     2821 agcagcgaca gggcagccac cacctttgcc cggggatcca tcccctgaag cagcccgggc
     2881 aaccgggcct gctcctcgct gaagagcgct cgctccagcg cacccgacac accggccacg
     2941 gtgcgagcca cgaagtcccc gccgccgcgg cggcgagact gccgcacaag aggttggtcc
     3001 atactcccgc taatccctgc gcgccgcgga ggccggtcgc cccagcgcca cgcgcgccgc
     3061 gaaaagcagc acaaagatgc acgccacccc aaccacggcg gagagaatgt aggcaggagc
     3121 ctgctgcagg aaggaggagt ccggccccat gccgggcgcc tggtagccac gcaagggggc
     3181 caactgccac gaatcggcca gccgggctaa cccgctgggc acgtagccca ccattccctc
     3241 caactccgat tttccccact cgaactccgc tccgcccggc gccagcaacc ccaggggcgc
     3301 aagaactacc atggcgccca ggaccgacac caacgaccac cccgcccgcc cgaggccatt
     3361 gcccgacgcc ccggcagcgg cctcgcccag cagcgccgga tgggatcgaa gcaggaaggc
     3421 cacccccagc cccgtcagcg cagcctcggc aaagcccgcc agggtcaggt gcaccagcat
     3481 catggccggc agggtgacgt cgaggccgta ggggttgtag agggccctcc ctccctcgct
     3541 ccacagcagc ggctggatgc ccaactccag ccctgccagc agccccgcgg tgttgatccc
     3601 gatatagcca cccaccgccg ccgcgacggc cctgcgcctt ggggaggggc tgtgcccggc
     3661 gagcagtcgg tagagggcgt acccgaccag cggaagcgca atccccatat taaagcagtt
     3721 ggcgccgatg gctgtgatgc ccccatcccc aaagaagagg gcctggataa ccagggcggt
     3781 ggaaaccccg accaccgccg cccacgggcc aagcacgacg gccatgagcg tgccaccaac
     3841 ggcgtgggcg gtggtgcccc ccggcaccgg gatgttgaac atctggatgg tgaagctgaa
     3901 ggcggcgaat agcgccacca gtggtgccga ctgcccccgc agcgtctcct tcaccctctt
     3961 gccggcctga taccagaagg gcgctgccgc gccgtaggcg acggccacgg tcgccgggct
     4021 gaggtaacca tccggtatgt gcatctcaac ctcccgcaac tccgcagatg ccgcgagcta
     4081 accacgaaat acccgaaaca cacgaaatac gggccctgcc gccaggtagg ggcgacgcac
     4141 gcctcaccct atccagccgc tgccgccgaa atccgtaggg gcgacgcgtg cgtcgcccaa
     4201 gccagacgcc gttggcgatc ctccacgggc tgcaactgag ttgcaactcg tgagcaaaag
     4261 aaaaggtcag gtcctgcact ccgggcagag gccgaagact ggatagctct ccagctccac
     4321 ctggaagccg tgctcttccc ccaaccgtcg ccgtatcgcg gatgccaccg attcgtccat
     4381 atccagaagc cggccgcacc gccggcatat catatggcta tggggtccgt gggatgcggg
     4441 ctcgtactgg gcgcggccgt cgccgcactt atgctcagac accagaccca actccagcag
     4501 cagatccagc gcccgataca cggtggtctt gttcatccga agcccgggat ggtggaccgt
     4561 aaccgactcc aggatgtgct ccgcgcagat gtggccggag cagccgaaga tggcctcaca
     4621 gacggcctcc cgctgagtgg tcagccgaag tcccctgccc tggagcgccg ccactatttg
     4681 cccgcgggcc tcagtcgcca gccttttccc cacccctgga tccgtggagg tgcacatcga
     4741 cctctccccc ctgcctcacc agttgcaact cagttgcaga actgatacgc cattatacct
     4801 ggatggaccg gtgggctaca atctgcttga cttgatggcg ctccaaacgt acgatgctga
     4861 tgccaccgct tcgttccgtt tgctcgttca aaagggaata tccagcaaga aggggcgtat
     4921 tctgccagtt gcccggcgtt accttcgtta ggtccctccc aatgccagga gggatccctc
     4981 cacattctgg aaacacctga ctcagtgaag ggaaggtggt tgggatggtg aggtttgaag
     5041 tagttccgag atgcaacggc tgcaagtcgt gcgagatggc ctgctccttt cgggcgacgg
     5101 ggtgcttctg gcctccggcc tccgcgatcc acacccggag aacgggctcg aagggcgcgt
     5161 atcggtacga gatcgggata gacgccgacg agtgcgacct gtgcgagaat gaggcaatcg
     5221 ccctctgcca gaaggcatgc ggcccatgca tattgaccgg ggaggtcatc tctcgcatcc
     5281 ggggagaggt cgccgtgtag ggtttggaaa gcctacagga ccggcaccgg agacggcttg
     5341 tctctcttag ctggacagcc atagacccaa gacgctatca tctggccgcg tcgaatatcg
     5401 agccagggtg aacaggcttc ta
//
LOCUS       DATALS010000278         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_58062,
            whole genome shotgun sequence.
ACCESSION   DATALS010000278 DATALS010000000
VERSION     DATALS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58062"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..173
                     /locus_tag="VHS06_06120"
     CDS             15..173
                     /locus_tag="VHS06_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987731.1"
                     /translation="MNREGEIVHLVREMGAFRMSRYVFSARTVLELPAGTIQESGVRV
                     GDRLVLED"
     gene            163..849
                     /locus_tag="VHS06_06125"
     CDS             163..849
                     /locus_tag="VHS06_06125"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX2987732.1"
                     /translation="MKIDPQRLLAFLFPSRCVGCGLRGVDLCERCFSTIRPLDSTCCP
                     RCAGPSRFGAICPSCRRYDGPMAGVRAATSYDGVVRKAIHSFKYRHRQTLAASLAALV
                     GMEFRRRPLQVDILVPVPLHPRRLAARGYNQSALLAGELSSLTGAPVADLLERGRETA
                     AQAGLKASQRRTNVRGVFRCSGSAELVGARVGGVDDVCTTGATLEDCARALREAGCTS
                     GWGIVVARDL"
     gene            1009..2370
                     /locus_tag="VHS06_06130"
     CDS             1009..2370
                     /locus_tag="VHS06_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987733.1"
                     /translation="MAQKGADRMGDEAVSQVPGPLRSSAALSPDDAKLPVHSPKSSND
                     RPTTPNPLSSIPYPPSPTPRCGGLGTTRLLELVLLAGIFILNFFMSTDPDMWWHMAAG
                     RFILETGTVPQTDPLSYTAAGWPWVAHEWLAEIAMYLLYQAGGYLLPVVVFAALVTAT
                     FWVLFRTLRLLAMSAAASAAITFWAAFMSLAGWNVRPQIFSYLFFAIYLFLLLRSRQR
                     PDRLIWAMPAIMALWVNLHAGYVMGLFIIGCFVVGEGINQLRVVSRQRSAISTQHSSW
                     LGAARRCARLGRVGGDPPHWKAPNTQHTALSTQFRQWIAVGVATLAATAINPQGLSML
                     LYPLTYAGTQNASMRYITEWQSPNFHDYFYFVFGASLMVLMLLPGRRAVDWALGIPLL
                     LLTAMSLQSARVIPFYAIAMAPFLALRLRPPALGGPHPAFGRPLPEGEAVTEFPLSGR
                     GSG"
     gene            2410..>2602
                     /locus_tag="VHS06_06135"
     CDS             2410..>2602
                     /locus_tag="VHS06_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX2987734.1"
                     /translation="MTAQLLQGQPVAEQIRAELAEELFNFKREYGVTPQLAIVQVGDN
                     SAATAYVRRIEKAFAAAEMV"
BASE COUNT          456 a          823 c          856 g          467 t
ORIGIN      
        1 tcgatgcact cttcctgaac agggagggag agatagtcca cctggtgcga gagatgggcg
       61 ccttccggat gagccgctac gtcttcagcg ccagaacggt gctggaactc cctgccggca
      121 ccattcagga gagcggggtt cgagtaggag acaggctggt acttgaagat tgacccccaa
      181 cggcttctgg cgttcctctt cccctcgcgg tgcgtcgggt gcggactccg aggggtagat
      241 ttgtgtgagc gctgcttctc cacgatccgg ccgttggatt ccacctgctg cccacgctgt
      301 gccggcccct cgcgattcgg ggccatttgt cccagctgcc gccgctacga cggccccatg
      361 gccggggttc gtgccgctac cagctacgac ggggtcgtca gaaaagcgat ccacagcttc
      421 aagtaccgcc accggcaaac cctggccgcc tcattggcgg ctttggtcgg gatggagttc
      481 cgccgaagac cgctccaggt ggacattctg gtgccggttc ccctccaccc cagacggctg
      541 gccgcgagag ggtacaacca gtcggcgctg ctggccggcg agctatcctc gctgacagga
      601 gcgccggtag ccgacctgct cgaaaggggc agggagacgg ccgcgcaggc tggactgaag
      661 gcgtcccaac gccgaaccaa cgtgcggggg gtcttccgct gctcagggtc cgcggaactg
      721 gttggggcga gggtgggagg tgtggacgac gtctgcacaa ccggcgcaac gctggaggat
      781 tgcgcccgag cgctgaggga agccggatgc acctccgggt gggggatcgt ggtggcgagg
      841 gacctctgac gggggatggg ggttggggga ccgaagaccg aagacgaggg acgagggacg
      901 agggacgagg gacgagggac gagggacgag ggacgaggga cggaaagatg gtactacgga
      961 tgtggccggg cgtggccgaa gattcttgga gaggggatga gtaggtagat ggcgcagaaa
     1021 ggggcagata gaatgggcga cgaagcagta agccaggtac ccggcccgct gcggtcgtct
     1081 gccgccctct ccccggacga cgccaagctc cctgtccact cgccgaaatc atccaacgac
     1141 cgacccacaa cccccaatcc cctatcctcc atcccctatc ccccatcccc caccccccgc
     1201 tgcggcgggc tgggcactac gaggctgctg gagctggtgc tgctggccgg catcttcatc
     1261 ctgaacttct ttatgtcgac cgaccccgac atgtggtggc acatggcggc cggaaggttc
     1321 atattggaga cgggcacagt cccgcagacg gatcccctct cctacaccgc cgccggctgg
     1381 ccctgggtgg cgcacgagtg gctggccgag atagccatgt accttctcta ccaggccgga
     1441 gggtatctgc tgccggtagt cgtcttcgct gcgctggtaa cggccacctt ctgggtgctg
     1501 tttcgaacac tgcggcttct ggcgatgagc gccgcggcat ccgcggccat cacattctgg
     1561 gccgccttca tgtcgctggc aggctggaac gtacgcccac agatcttctc ctacctcttc
     1621 ttcgccatct acctgttcct tctactgcgc tccaggcagc ggccggaccg attgatctgg
     1681 gcgatgcctg ccatcatggc gctgtgggtg aacctccatg cgggctacgt catgggacta
     1741 ttcatcatcg gatgtttcgt ggttggggag gggatcaacc aacttagagt tgtcagccgt
     1801 cagcggtcag cgatcagcac tcagcactca tcgtggctcg gggcggcgcg acgctgcgct
     1861 cggctcggga gggtcggggg cgatccgccc cactggaaag cacccaacac ccagcacaca
     1921 gcgctcagca cacagttcag gcagtggatc gcggtggggg tcgcaactct ggcggcgacc
     1981 gcgatcaacc cgcaggggct ctcgatgctg ctctaccccc tcacctacgc cggaacgcag
     2041 aacgcctcca tgcgatacat cacggagtgg cagtcgccca atttccacga ctacttctac
     2101 ttcgtcttcg gcgccagcct gatggtgttg atgttgctcc cgggacgaag ggcggtcgac
     2161 tgggctctgg ggatcccgct cctcctgctg acggcgatgt ccctccaatc cgctcgggtc
     2221 attcccttct atgccattgc catggcaccc ttcttagcgc taaggctgcg cccacccgcg
     2281 ctggggggcc ctcacccggc cttcggccgg cctctcccag agggagaggc ggttacggaa
     2341 ttccctctct ctgggagagg gtcagggtga gggcagcact cagcacccag caccatcgga
     2401 gggggtcgca tgaccgcaca attgcttcag ggccagccag ttgccgagca gatcagggcg
     2461 gaactggcgg aagagctgtt caacttcaag agggagtacg gggtcacccc tcaactcgcc
     2521 atcgtccagg tgggagacaa ctcggccgcg accgcctatg tcaggcggat cgaaaaggca
     2581 ttcgcggccg cggagatggt tt
//
LOCUS       DATALS010000279         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_252509, whole genome shotgun sequence.
ACCESSION   DATALS010000279 DATALS010000000
VERSION     DATALS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252509"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..834)
                     /locus_tag="VHS06_06140"
     CDS             complement(52..834)
                     /locus_tag="VHS06_06140"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX2987735.1"
                     /translation="MRENKVKKILNSGGTVVGTMISEVRGPGVMHMLGTAGLDFVYID
                     MEHSTYSMETVGDMILAAKATDMMAFVRVPGYENRCALSHPLDAGAEGLLCPQTESVE
                     QAEYIIRETKYYPMGQRGAALRRGHSAFAGVKFGEYAPHANAETMIILQIESEKGVQD
                     IDRLMAIDGVDAAFIGPADLSQSYGIPGQAKDPRIIEAIHKVLAGCQKAGKACGIHLY
                     NMDDVKYWTQAGMRLICYNNDIAMITDTASKFNKEIREVVGR"
     gene            complement(837..1538)
                     /locus_tag="VHS06_06145"
     CDS             complement(837..1538)
                     /locus_tag="VHS06_06145"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2987736.1"
                     /translation="MTAGQTFRLETYRTLQELVYQSLREAILDGRLVPGQRLVAGNLA
                     AELGVSRMPVREALSRLQQDGFVKATPHREVVVAGFSERDVVEIYDIRSVLEAHAAGL
                     CAVHATPGELEQLRELVGGMQQALEDGDLERVRRLDNDFHHTLFNTCGSGQLAQLLTQ
                     LWDQCLYYRSLAVSLNRNPARSLEHHQQIVAALERHDHQNAEKWIHTHTQVARDTLLD
                     HLRARRKSQEKDREA"
     gene            complement(1577..1849)
                     /locus_tag="VHS06_06150"
     CDS             complement(1577..1849)
                     /locus_tag="VHS06_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987737.1"
                     /translation="MDSDTKIDPFLMLSSGPIITEHSFASNRSPAGFEAIAAFLGWSS
                     TAAAYSSHGRTRREFLKVQEEFGGIDIIITTDALYPISDMTYPMPE"
BASE COUNT          377 a          658 c          599 g          433 t
ORIGIN      
        1 actcatcgga gggggtcacc atcgccggga tgatgccttt cgggacgaag ttcatcgacc
       61 cacgacctcc cggatctcct tgttgaactt ggaggcggtg tcggtgatca tggcgatgtc
      121 gttgttgtag cagatgagcc gcatgccggc ctgcgtccaa tacttgacgt cgtccatgtt
      181 gtacaagtgg atgccacagg ccttgcccgc cttctggcag cccgccagca ctttgtggat
      241 cgcctcgatg atgcgcggat ctttcgcctg gccggggatc ccatacgact gggagaggtc
      301 cgccgggccg ataaatgcgg cgtccacccc atcgatggcc atgagtctgt caatgtcctg
      361 aacacccttc tccgactcga tctgaaggat gatcatggtc tcggcgttgg cgtggggcgc
      421 atactcgccg aacttcaccc cagcgaaggc cgagtgaccc cggcgcaggg ccgcgccacg
      481 ctgtcccatc ggatagtact tggtctccct gatgatgtac tcagcctgct ccacggattc
      541 ggtctgtggg caaagcagcc cctcggcgcc ggcgtccagc ggatgagaga gggcgcagcg
      601 attctcgtag cccgggacgc gaacgaaggc catcatgtcc gtcgccttgg cggccaggat
      661 catgtcgccg accgtctcca tcgagtaggt cgagtgctcc atgtcgatgt acacgaagtc
      721 cagccctgcc gttccgagca tgtgcatgac tcccggcccg cggacctccg agatcatcgt
      781 cccgacaact gtcccgcccg agttcagaat cttcttgacc ttgttctcgc gcatgtctac
      841 gcctccctat ccttttcttg agacttcctt cgcgctctca agtggtccag gagggtgtcc
      901 cgggccacct gcgtatgcgt gtgaatccat ttttcggcgt tctgatggtc atgccgctcc
      961 agcgccgcca cgatctgctg gtggtgctcc aacgaccggg ccgggttgcg gttgaggctg
     1021 acggcgaggc tccggtagta gaggcactgg tcccaaagct gcgtcagcag ttgggccagt
     1081 tggccggagc cacaggtgtt gaagagagtg tgatggaagt cattgtcgag ccgtcggacc
     1141 cgctccagat cgccatcctc aagcgcctgt tgcatcccgc cgaccagttc cctgagttgt
     1201 tcgagttccc cgggagtggc gtggacggcg cacagaccgg cagcgtgagc ctccagaacc
     1261 gagcggatgt cgtagatctc caccacgtcc cgttccgaga accccgccac cactacctcc
     1321 cggtgaggcg tggctttgac gaacccatcc tgctgaagac gggacagggc ctcccgaacg
     1381 ggcatccggc taacccctag ttctgcagct aagttgccgg cgaccagccg ttgaccgggg
     1441 accaagcgtc cgtcgaggat cgcctcgcgc aggctctgat agaccagctc ctgcaaggtg
     1501 cgatacgttt ccaacctgaa cgtttgcccg gcagtcatcc gatctccccc agtcaagtca
     1561 ggccccggtg cagaggctac tctggcatgg gataagtcat atcggatatt ggatacaatg
     1621 cgtcagttgt gataattatg tctattccgc caaattcctc ctgaaccttc aggaattccc
     1681 ttctcgtcct accgtgtgaa ctgtacgcag ccgctgttga cgaccatccg agaaaggcgg
     1741 caattgcctc aaagccagct ggcgagcgat tgctggcgaa cgaatgttcg gttataatgg
     1801 gtccgctgga taacattagg aaggggtcga tcttcgtgtc gctgtccact gcccgcctca
     1861 actacctggt gaatcacatc ccagactacg ccctggagca ggtccccaaa tcatcggacg
     1921 gacggccacc ctgtccccac tgtcacggat ccgcggcgta cagttggggc acgttcgccg
     1981 gccggtcgcg ctaccggtgc aaacagtgtc gcaggacgta taccgccctt accgccacac
     2041 ctctggccaa gctcaggtac cgtgacc
//
LOCUS       DATALS010000280         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_103575, whole genome shotgun sequence.
ACCESSION   DATALS010000280 DATALS010000000
VERSION     DATALS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103575"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1074)
                     /locus_tag="VHS06_06155"
     CDS             complement(169..1074)
                     /locus_tag="VHS06_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEX2987738.1"
                     /translation="MSTDGGGVSRGEAIRVRLESLKAQSPGMEDAIAFYQQLLPALLE
                     AQDTVSPIAIEREVADARLRSGTPLLVGEDLPLNLEETRYTLLRLCRLVEALPSAKHG
                     IDGDLPRAQQAEAIRSSVESGALDPEKVWDALLSGNQGRLDSIATAHGLHLGMLQLLA
                     QFSLRPSFRGWAESLKESADWSLWERGACPICGSDPGLAELQGKERAPHLRCTNCGAD
                     WPYPRVKCAFCGNDEPQSLHIFSLSGEEERRQVQACDRCHGYIKRIATFDPTPVEMLQ
                     VEDLAALHLDIMAADKGFRRGTVNR"
     gene            complement(1240..2145)
                     /gene="nrfD"
                     /locus_tag="VHS06_06160"
     CDS             complement(1240..2145)
                     /gene="nrfD"
                     /locus_tag="VHS06_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX2987739.1"
                     /translation="MAGELQIPHWEWWIVLYFFMGGVAGGAYFLSAVLGLVGTPEDRP
                     VARLGYYIAFPAALVCALLLIVDLGRPERFWHMMVYSNDLLPWPKWDSPMSVGSYALL
                     FFGGFSFLSFLDALVETGRIPWAPFRLHYTGWPSMVYSVVGGLFGFFVTAYTGELLAT
                     SQLPAWENNPFLGALFAASGASTGMAAIALVAVLTGRHVGRSWDKLRRADSVAIVFEF
                     LALLAFLAALGASALVFLSGVNALVLVGGVVLVGLTFPLVCQIRCGFRESHLSQRMTA
                     VTAVLVLLGGLCLRTTIVMGGQGLL"
     gene            complement(2148..>2496)
                     /locus_tag="VHS06_06165"
     CDS             complement(2148..>2496)
                     /locus_tag="VHS06_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987740.1"
                     /translation="AKVCPTGSIAFGDVDALRVKARQRVSTLQSQGYQKAQLYGDTNI
                     LGGLNVMYLLLDDPEVYGQPRNPQVPQRGLVSSSLVSIGTAVALGVSALVAFRERGKG
                     PGIGPAKVGKKEE"
BASE COUNT          503 a          849 c          719 g          425 t
ORIGIN      
        1 ggggaaagag agggcaccgc atctgcgctg caccaattgc ggagcagatt ggccctatcc
       61 gcgggtcaaa tgtgcctttt gcggcaacga tgagccacag agccttcaca tcttcagcct
      121 cagcggggag gtcgaaaacg caaatggcga ggtgaacagg gcagtcggct accggtttac
      181 agtgccccgc cggaatccct tatcagcagc cattatgtca agatgcaggg cggccaggtc
      241 ctctacctgc agcatctcca cgggtgtggg atcgaaggtc gcgattcgct ttatgtagcc
      301 gtggcatcgg tcgcacgcct ggacctgccg cctctcctcc tccccgctga ggctgaagat
      361 gtgaaggctc tgtggctcat cgttgccgca aaaggcacat ttgacccgcg gatagggcca
      421 atctgctccg caattggtgc agcgcagatg cggtgccctc tctttcccct gaagctcagc
      481 gagcccagga tcgctgccgc atatgggaca tgcccccctc tcccagaggc tccagtccgc
      541 gctctccttc aggctctctg cccagcccct gaaggaagga cgcagggaga actgcgcgag
      601 gagttgtagc atcccgagat gcagcccatg ggcggtggct atgctgtcca atcggccctg
      661 gtttccggat agcagggcgt cccacacctt ctccggatcc agcgcaccac tttcgacaga
      721 actgcgaatc gcctccgcct gttgggcacg aggcagatca ccgtcgatcc catgcttagc
      781 cgacggcagc gcctccacga gccggcatag ccgcaacagg gtgtagcggg tctcctccag
      841 attcagcggc agatcctcac ccaccaggag tggcgttccg gagcgcagtc tggcgtcggc
      901 cacctcccgt tcgatagcga taggggatac ggtgtcctgt gcctccaaca gcgccggaag
      961 gagttgctgg tagaaggcga tcgcgtcctc catcccgggg ctctgggcct tcaacgattc
     1021 cagtcgcacg cggatcgctt cacccctgga cactcccccg ccgtctgtgc tcattttgaa
     1081 gtcccacctt tccgaagttg ccggctgcca acagatacgt ccgacggcct tgtgtccagg
     1141 agcccgggtc gtccctgacc cgggcgtaat accgttggtt tgccatggcc cgggcgggtc
     1201 aggggcgacc cgccctaatc cctccgcgag caggttgtgt tacagcagcc cctgccctcc
     1261 catgacgatg gttgtccgca ggcacaagcc tcccagcaat accaggaccg ccgtcacagc
     1321 cgtcatccgc tgggagaggt gcgactcccg gaagccgcac ctgatctgac acaccagcgg
     1381 gaacgtaagg ccgacgagca cgactccacc taccaacact agggcgttca ccccactcag
     1441 aaagaccaag gcggatgctc ccaacgccgc caggaacgcc agcagcgcaa ggaactcgaa
     1501 cacgatcgcc acgctgtctg ctcggcgcag cttgtcccac gacctgccca cgtgccgccc
     1561 ggtcagcacc gctaccaggg caatggccgc cattcccgtg gacgcgccgg aggccgcgaa
     1621 cagggcaccg aggaacggat tgttctccca ggcgggaagc tggctggtgg ccagcaactc
     1681 gccggtgtag gctgtcacga agaagccgaa cagccctccc accaccgaat agaccatgct
     1741 gggccagcct gtgtagtgca gccggaacgg agcccagggg atccgtccgg tttccacgag
     1801 cgcatccagg aacgagagaa agctgaagcc accgaagaag agcagcgcat aggacccaac
     1861 cgacatgggg gagtcccact tgggccacgg cagcaaatcg ttggagtaca ccatcatgtg
     1921 ccagaagcgc tccggcctgc ccaggtccac gattagcagc agggcacaca ccagcgcggc
     1981 cggaaaggcg atgtagtagc ccagccgcgc cacaggccta tcctccggcg tgccaaccag
     2041 cccgagcacg gcggacagga agtaggcacc tccggcaacg ccgcccatga agaagtagag
     2101 aacgatccac cattcccagt gggggatttg caactcgccc gccatctcta ctcctccttc
     2161 tttcccactt tggccggtcc aataccaggt cccttgcccc gctctcgaaa agccaccaac
     2221 gcagacacgc ccagggccac cgcggtccct atactgacga gagaacttga caccaagcct
     2281 cgctgcggca cttgtgggtt ccgcggctgg ccatatacct caggatcgtc cagcagcagg
     2341 tacatcacgt tcaggccgcc gaggatattc gtgtcgccat agagctgcgc cttttgataa
     2401 ccctgactct gcaaagtgga cacccgttgc ctggctttga ccctcagtgc gtccacgtcg
     2461 ccaaaggcta tggatcccgt cgggcacacc ttggct
//
LOCUS       DATALS010000281         9995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_28923,
            whole genome shotgun sequence.
ACCESSION   DATALS010000281 DATALS010000000
VERSION     DATALS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28923"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VHS06_06170"
     CDS             complement(<1..776)
                     /locus_tag="VHS06_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2987741.1"
                     /translation="MRLHEFKLERYFARWEFDAPYLLCCSDLEGYRLDDLLALADDES
                     RELWHNLALGYTESAGHPLLRAEIARLYDGVSPEEILTFAGAEEAIFVLMNVLLGQGD
                     HAVAVWPAYQSLHEVARAAGAEVTLLPLEESNGWALDLNALRRAMRPNTRLIVVNFPH
                     NPTGYLPDSATFSALLELAEESGATLFSDEVYRLLEFDPAYRLPAAVDRSRRAVSLGV
                     MSKAFGLAGLRIGWVASHDHDLLARMAAYKDYTSICNSAP"
     gene            1021..2280
                     /locus_tag="VHS06_06175"
     CDS             1021..2280
                     /locus_tag="VHS06_06175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2987742.1"
                     /translation="MAVKETVYRYRWVMLGIAFVVQFSNALATQAVAPLAPLFQPELG
                     LTKSEVGLFSSIIFAGSWGVLVVAGSLCDRFGLRRLMSVALVIVGLVVLYMVRVGSFF
                     EAAAVMFLAGVGGGVVMPGSTKAIMDWFPPRARATGMGIKQAGVPLAGIVTASTLPVI
                     GLTVGWRAAIAGVGFAIIASGVIACLFCRDLPRTTKEVERKTPFRVVFREVVKNRGLW
                     AVSSISVLYVTAQLSLITYVALFLEEVVLVDAIPDEHARIVAAGGYLALCQAGGVVGR
                     IFWGVVSDRVFHGRRMPVMAVIGTLSAALSLLMAVMGPGFPLVVMAPMMFLFGATAIG
                     WNGIYHAAMAETSGQKYAATGTGMSMTLNQFGVVGGPPLFGFIVDISGSYPAGWAMLA
                     VCCAVGVVVSLFRAKGEVRNDEAALGR"
     gene            2255..3181
                     /locus_tag="VHS06_06180"
     CDS             2255..3181
                     /locus_tag="VHS06_06180"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX2987743.1"
                     /translation="MTRRLWDGRVESAGGIAGVRLAVGAALLLLVCLLIPPAAGAREL
                     VTPDVGTLTQTALGVDDPEAIAQWIDTEAVPIPQEPQEALSTLRARGASGWSRFRTPF
                     PKVPVWNPPCPKRVGLQAGHWLFNEAPDELADLRSNPGTSGGGKAEWEVTLDIAQRTA
                     TLLQGAGVQVDILPTTIPVRYRANAFVAIHADGDNGGALNGYKVAGSGWTGTPEADAA
                     LVDAINQEYAATTGLPRRDEQISLRMRWYYGFNARRYQHAVAPGVPQAIIETGFLTNA
                     GDRQLLIGNPDRIAAGIAAGILDYLNWEAATQ"
     gene            3178..3534
                     /locus_tag="VHS06_06185"
     CDS             3178..3534
                     /locus_tag="VHS06_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987744.1"
                     /translation="MMARSPREFCEAMRDLVVRMQHHPATAVSDQAYEMKLRVLEYLI
                     AKNPNEEGFERLLMERIDDRDPAKELSRGICCQVLNSWRAGSCHYTPEGRLVLRALYP
                     ADQSADFEGDDAKLEE"
     gene            3537..4679
                     /locus_tag="VHS06_06190"
     CDS             3537..4679
                     /locus_tag="VHS06_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2987745.1"
                     /translation="MVMESAALDARRRADEGRSGSLVKVTDLKVRRGETLVLDIPGLE
                     ISEGEVLAVIGPNGAGKSTLLHTLALLLPASSGEISFGGRSISKGDDLVQFRRRMAVV
                     FQEALLLNCSVRENVAAGLKLRGVPKGKAEERASYWMKRFGVDSLADRPSRQLSGGEA
                     QRVSLARAFALEPELLLLDEPFSALDAPTRAALFEDFERVLRDSGITTVFVTHDRMEA
                     MRLGDRIAVVIGGCIAQVGEPEAVFSTPASQQVAAFVGMENIIPGRVSEQREGLALVS
                     LRSSMTIEVVSDAPAGQRVLACLRPEDVVVAPMPSELPSSSARNKLRARITRITPLGS
                     QSRLSLDCGFPLEALITRRSAEELSLQVGLEVLASFKATAVHLLSR"
     gene            complement(4669..5160)
                     /locus_tag="VHS06_06195"
     CDS             complement(4669..5160)
                     /locus_tag="VHS06_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2987746.1"
                     /translation="MLKTVHAESDVHLKAIRELFLEYASSLDFDLDFQDFGEELAGLP
                     GKYSPPEGRLILVETEIGFIGCVALRPLEDKICEMKRLYVRPEARGQGVGRLLGETIV
                     REARRAGYHRMRLDTVEWMTEAKALYRSLGFATIAAYCYNPIEGAEYMELLLQDPHTK
                     TTC"
     gene            complement(5164..5718)
                     /locus_tag="VHS06_06200"
     CDS             complement(5164..5718)
                     /locus_tag="VHS06_06200"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX2987747.1"
                     /translation="MGSIGFRKAIETETRQLSDLEKRLQDKIRHGETISRNVNRLHDD
                     QLTFGQRLADRLAEVAGSWNFIIVFLILLAIWIGTNSFLLIAQPWDPYPFILLNLLLS
                     FLAGLQAPVIMMSQNRQESKDRIRAEHDYEVNLKAEMEIQELHRKIDQLRETQWAGLV
                     EMQERQIALLEEQIRLLQEMQGRG"
     gene            complement(5847..6989)
                     /locus_tag="VHS06_06205"
     CDS             complement(5847..6989)
                     /locus_tag="VHS06_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987748.1"
                     /translation="MRRLLIALALTLAVALPMVEGTEAQAQPQFQFGFSTLASLIPSI
                     VGQPLENEHFDANSGASLQRTGNGLMVWRKADNVTAFTDGSTTWVIGPQGLQTRPNDT
                     RFDWETAAAPTSTQTVTNGVSLTFSRAADGSWAGRGNVQNTLGVTEDVEVNVVGFDPS
                     SGNPVIDAPTVYVVGLAQGGSVPIRVSVPTTANAVNWQTFVTSHPTSADANFTMDVGA
                     SQPLSVDPALAGAITELRRVDNGDALVKSAADNGVVISESSLPASILGGFAPEESEIT
                     IAAALEDSSAWVRATVLAHELQHAADAASGFVPQTTQQCLQEETNAFQRQSVVWNDFW
                     RNRLPAGVDQLHAELNDVTNTVDNNPQAFAALLARRYRSECGASRQ"
     gene            complement(7261..8757)
                     /locus_tag="VHS06_06210"
     CDS             complement(7261..8757)
                     /locus_tag="VHS06_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX2987749.1"
                     /translation="METFGLLMNGFATAMQPTNLIFAVMGCLMGTMIGVLPGVGPAAG
                     TAMLIPVTFVLDPTPAIIMLAAIYYGAMYGGTITSVLVNVPGEAASAITCIEGYEMAK
                     RGRAGAALSIAAIGSFIGGGIATLGLVVLALPLTALALKFGPPEFFALMVMGLSLVTG
                     LASKSMVRALMAAVFGLMIAMVGIDPVMGAPRFNFGQTELLDGFNFVPVVMGLFGISE
                     IFANAEHPIADVFETSLSSLIISWKDLKASIWPIIRGTFIGFFLGLIPGVGAVVPTFM
                     SYVAEKKTSKYPEKFGTGLIEGVAGPETANNAYANAAMIPLFTLGIPGSPTIAILMGA
                     FMMNGLIPGPFLFKEHADFVWAVIASMYVGNLILLILNLPLIPLWVALLKVPYSVLFA
                     IILGFCVLGSYSIDSSVFDVGVMLAFGVVGYILKKLDFPLAPICLTLILGPLMERGLR
                     QSLEISRGDFGIFFQRPIALGLLIISVLFVLGATFQMISKVQGDDSQL"
     gene            complement(8767..9225)
                     /locus_tag="VHS06_06215"
     CDS             complement(8767..9225)
                     /locus_tag="VHS06_06215"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX2987750.1"
                     /translation="MKRPYQITAVVFILFAAFIARDSMELKFYTALGPGPSFFPFWLS
                     VLMMILAAMMFFNATFKNAGPMPDDFWASKVGYLRMLAIFLAISFAVFFMETIGFRLT
                     MLAFYLFLLFALGRQNLIVTAIVAIAGSWGVFQVFTEYLKVPLPIGIFGI"
     gene            complement(9796..>9995)
                     /locus_tag="VHS06_06220"
     CDS             complement(9796..>9995)
                     /locus_tag="VHS06_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004010298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2987751.1"
                     /translation="VIDEIRSRGTTILLVEQNASMALDLADRGYVMELGKIVLDGPAT
                     DLAESDLIKHSYLGTGAEGLF"
BASE COUNT         1905 a         2849 c         3262 g         1979 t
ORIGIN      
        1 ctcggcgcgc tgttgcagat ggaggtgtag tccttgtagg ccgccattcg agcaagcagg
       61 tcgtggtcgt gggaggcgac ccagccgatc cgcagccccg caagcccgaa ggctttggac
      121 atgaccccca ggctaacggc acgacgcgac ctgtccaccg cagcgggcag ccggtaggcc
      181 ggatcgaact ccagcagccg gtacacctcg tcggagaaga gtgtcgcgcc cgactcctcg
      241 gccaattcga gcagcgccga gaaggtcgcg ctgtccggga ggtagccggt gggattgtgg
      301 gggaagttca ctacgatcaa gcgagtgttt gggcgcatcg cgcgtcgcag agcgttcaga
      361 tcgagggccc agccgttgga ctcctccagt gggagcagcg tcacctcggc gccggcagcg
      421 cgagctacct cgtggagtga ttggtaggcc ggccatacgg ctaccgcgtg gtcgccctgc
      481 cccagcagga cgttcatcag aacgaagatc gcttcctcgg ctccggcgaa ggtgaggatc
      541 tcctccggcg aaaccccgtc atacagacgc gcgatctcgg ctcgcagcag ggggtgacct
      601 gccgactcgg tgtagccgag ggcgaggttg tgccagagct cacggctctc gtcatcggct
      661 aatgccaaca gatcgtccag acggtagccc tccaggtcgg agcagcaaag caggtatggg
      721 gcgtcgaact cccagcgagc gaagtagcgt tccagcttga actcatgaag cctcaacgtc
      781 tgggcctcca taggaggatg tacatgcgag ggaagtgtac cacgacgggg acttagaggg
      841 actcctggaa ctctgaggag ctgagagggc cgtggtgcta gggcttgaca gccgctccat
      901 tcccagaagc gcaaggctcc acgagttccc cagagttccg caaaaggtat agaattacat
      961 acttcgtaag ccgttacccc gcacgttccc cactatcgtg gtgaaggagc catagagact
     1021 gtggccgtga aagagaccgt ctaccgttat cgctgggtga tgctgggcat cgcctttgtc
     1081 gtgcagttca gcaacgctct ggctacccag gcagtagctc ccctagctcc gctgtttcag
     1141 cccgagcttg gtctgaccaa gtcggaggtc ggcctcttct cgtctatcat cttcgctgga
     1201 tcgtggggcg tattggtggt tgccggctcg ctgtgcgatc gcttcgggct tcgacgcctg
     1261 atgtccgttg cactggtgat cgtgggcctg gtcgttctct acatggtcag ggttggctcc
     1321 ttcttcgagg ccgcagccgt gatgtttctc gcaggggtcg ggggtggagt ggtgatgccc
     1381 ggctcgacca aggcgatcat ggattggttc ccgccgcgcg ccagggcaac agggatgggg
     1441 atcaaacagg ccggcgtgcc gctggccggg atcgtcacgg catctacctt gcctgtaatc
     1501 ggattgacgg tcggctggcg agcagccatc gctggagttg gcttcgccat catagccagc
     1561 ggcgtgattg cctgcctgtt ctgccgggat ttgccccgaa ccaccaagga ggtcgagagg
     1621 aagaccccct tcagggtcgt ctttcgtgag gtggtgaaga accgcggatt gtgggccgtc
     1681 agctccatat cggtgctata tgtcaccgca caactgtcgc tcataacgta tgtggcgctg
     1741 ttcctggagg aagtggtgtt ggtggacgcg atcccggacg agcacgcgcg gatagttgcg
     1801 gcggggggct atctggccct atgccaggcc gggggtgtcg tcggtaggat cttctggggc
     1861 gtagtgagcg acagagtgtt ccacggcagg cgcatgcctg tgatggcagt catcggtacc
     1921 ctgtccgcgg ccttgtcgct gctgatggca gtgatgggcc ccggtttccc gctggtggtt
     1981 atggctccga tgatgttcct atttggcgcc acggccatcg gttggaacgg gatctatcac
     2041 gcggccatgg cggagacatc gggccagaag tatgccgcca ccggaaccgg gatgagcatg
     2101 acgctcaacc agtttggggt tgtgggtggg ccgccgctgt ttggtttcat cgtcgacata
     2161 agtggttcat atcccgcggg atgggccatg ctggcggtgt gctgtgccgt cggtgtggta
     2221 gtatcacttt tcagggccaa aggagaggtc cgtaatgacg aggcggctct gggacggtag
     2281 agtcgagagt gccggtggga ttgccggcgt taggttggct gtgggcgcgg cacttctcct
     2341 gttggtatgc ttgctgatcc cacctgcggc gggagcgcgg gagttggtca cacccgatgt
     2401 ggggacgttg acccaaacgg cactgggcgt ggatgatccc gaggctatcg cccagtggat
     2461 cgacaccgag gcggtcccca ttccccagga acctcaggag gcgctatcga ccctgagggc
     2521 tcgcggtgct tcgggatggt cacgatttcg cactccattc ccgaaggttc cagtgtggaa
     2581 cccgccctgc cccaagcgcg tcggtttgca ggccggccat tggctgttca acgaggcccc
     2641 agacgaactt gcagacctga ggagcaaccc agggacttct gggggcggga aggcggagtg
     2701 ggaggtcacc ctggacatcg cccaacggac cgccacgcta ctccagggtg cgggtgttca
     2761 ggtggatatc ctgcccacta ctattcccgt gcgctaccgg gcaaatgcct tcgtcgctat
     2821 ccatgccgat ggcgacaacg gcggtgcgct caatggctac aaggtggcgg gatcaggttg
     2881 gaccggtacc cccgaggcgg atgcagcgct cgtggacgcg atcaatcagg agtacgcggc
     2941 cacaaccggg ctgccccgtc gcgatgagca gatttcgctt cggatgcgct ggtattacgg
     3001 cttcaacgca cgacgatacc agcacgcggt ggcccccggc gtgccgcagg cgataatcga
     3061 gaccggcttt ctgaccaacg ccggtgatcg gcaactgctg atcggcaatc cagatcgcat
     3121 agcagcgggc atcgccgccg ggatcctcga ttacctcaat tgggaggcgg caacacaatg
     3181 atggctcgaa gccccaggga attctgcgag gcgatgcgag acctggtggt gcgtatgcag
     3241 catcatccgg ccacggccgt gagcgaccag gcctatgaga tgaagttgag ggtgctggag
     3301 tacctgatcg ccaagaaccc caatgaggaa ggcttcgagc gattgttgat ggagcgcatc
     3361 gacgatagag accccgcgaa ggagcttagc cgaggcatct gttgccaggt attgaacagc
     3421 tggcgagccg gcagttgcca ctacacccct gagggacggc tcgtattgag agcactctac
     3481 cccgccgatc aatctgccga ttttgaaggc gacgacgcga agctggagga atagtgatgg
     3541 tgatggagag cgcggcgctt gatgctcgaa gaagagcgga tgaggggcga tcgggctcgc
     3601 tcgtcaaggt gaccgatctg aaggtgcggc gcggcgagac gttggtgttg gacattcccg
     3661 gcctggagat ctccgaggga gaggtgctgg ccgtgatcgg tcccaacggc gctggcaaga
     3721 gcacgctgct tcacaccctc gcgctcctcc ttccggcatc ctccggcgag atcagtttcg
     3781 gcggtcgctc catatccaag ggcgacgatc tggtgcagtt tcgccggcgg atggccgtgg
     3841 tgttccagga ggcgctgttg ctgaattgct cggtcaggga gaatgtcgcg gctggtctga
     3901 agctgcgcgg cgttcccaag gggaaagccg aagagcgtgc ctcgtactgg atgaagcgtt
     3961 tcggcgtgga ttctcttgcg gataggccgt ctcgtcagtt atccggcggg gaggcgcaga
     4021 gggtaagcct cgcccgtgcc ttcgccctgg agccggagtt gttgctgctg gatgagcctt
     4081 tttccgcgct ggacgccccg acgcgggcag cactcttcga ggattttgag cgggtgttgc
     4141 gcgacagcgg catcaccacg gtcttcgtca ctcacgatcg tatggaggcc atgcggttgg
     4201 gagaccgaat cgccgtagtc ataggcgggt gcatcgccca ggtgggcgag ccggaggcag
     4261 tcttttccac tcctgccagc cagcaggtgg cggcttttgt gggtatggag aacattatcc
     4321 ccggccgggt ttcggagcag cgagaagggc ttgcactggt atccctccgt agctccatga
     4381 ccattgaagt ggtgagcgat gcccccgcgg gccaacgggt gctggcctgc ctgcgtccag
     4441 aggacgtggt tgtggcaccc atgccttctg agttgccatc cagcagcgcc cgcaacaagc
     4501 ttcgcgccag gataacccgc ataacccctc tcgggtctca atccaggctg tctctggatt
     4561 gcggtttccc cctcgaggcg ttgatcacca ggcgttctgc cgaggagctt tcccttcagg
     4621 ttgggctgga ggtgctggcc tcctttaagg caactgctgt ccacctactc agcaggtagt
     4681 cttcgtgtga ggatcctgaa gcaacaactc catgtactcc gcgccttcga tggggttgta
     4741 gcagtaagcg gcaatggtgg caaagccgag ggaacgatag agcgccttcg cttcggtcat
     4801 ccactcgact gtgtccagcc gcatccggtg atagccggcc cggcgggcct ctctcacgat
     4861 ggtttctccc agcaggcgac ctaccccttg ccctctcgcc tccggcctga cgtacagccg
     4921 cttcatctcg cagatcttgt cctcgagtgg gcgcaacgca acgcagccga taaaccctat
     4981 ctcggtctct accaggatca accttccctc ggggggagag tacttgcccg gtaagcctgc
     5041 aagctcttcg ccgaagtcct ggaagtcgag gtcgaagtcg agggatgagg cgtactcgag
     5101 aaacagctct cgaattgcct tcagatgcac atcgctttca gcgtggacgg tcttcagcac
     5161 tgcttaccct ctaccctgca tttcttggag aagcctaatc tgctcttcca gaagggcgat
     5221 ctggcgctct tgcatctcca ccagtcctgc ccactgagtc tctcttaact ggtcgatctt
     5281 ccggtggagt tcctggattt ccatctccgc cttcagattc acctcatagt cgtgctccgc
     5341 ccggatcctg tccttcgatt cctgacggtt ctggctcatc atgatcacgg gggcctgcag
     5401 cccagccagg aacgacagaa gcaggttcag aaggatgaac gggtagggat cccaaggctg
     5461 agcgatgaga aggaagctgt tggtgccgat ccagatcgcc agcagtatca gaaatactat
     5521 gatgaagttc caactgccag ccacttctgc aaggcggtct gccagtcgtt ggccgaaggt
     5581 gagttgatca tcgtgaagtc ggttgacgtt gcggcttatc gtctcgccat gtctaatctt
     5641 gtcttggagc ctcttctcga gatcggagag ctgcctggtc tcggtttcga tggcctttct
     5701 gaacccaatg ctgcccatgt gctctccttt cggcaacaca agtttcactg gttgagcaat
     5761 ggccggcgac tggaaacaat cataacatag aaggcagggc gggccttttc cggcccgccc
     5821 tgcgccgcgc cgtggagttg ggctcgttat tggcgcgatg ccccgcactc cgaccggtac
     5881 cttctcgcca gcagagcggc gaacgcctgg ggattgttgt ctacggtgtt cgtcacgtca
     5941 ttcaactcgg cgtggagttg atcgacgccg gcggggagac ggtttcgcca gaagtcgttc
     6001 cacacgacgg actgccgctg gaatgcgttg gtttcctcct ggagacactg ctgcgtggtc
     6061 tgcggcacaa agccggaggc cgcgtctgcc gcgtgctgca gttcgtgcgc tagcactgtg
     6121 gccctgaccc atgccgagga atcctccagc gccgcggcga tcgtgatttc gctctcttct
     6181 ggagcgaagc cgcccagaat gctggccggc aaggaggatt cgctgatgac cacaccgtta
     6241 tccgcggcgc tcttcaccag tgcgtcgccg ttgtccaccc tgcgcagttc agttatggct
     6301 ccggctaggg ctggatctac gctcagtggt tgacttgctc ccacatccat cgtgaagttg
     6361 gcgtctgccg aggttgggtg tgatgtgacg aaggtctgcc agttcacagc gttggctgtc
     6421 gtaggaacgc tcacacggat tgggacggag ccaccctgtg ccagtcccac cacatacacg
     6481 gtgggggcgt cgatcaccgg gttgccgctg gaggggtcga agcccaccac attgacctcg
     6541 acgtcctccg tcactcccag agtgttctgc acgttgcctc gccctgccca actgccgtcg
     6601 gccgccctcg agaaggtgag ggaaaccccg ttggtcaccg tctgcgtgct cgtgggcgcg
     6661 gccgcggtct cccagtcgaa tcgagtgtcg ttgggtcgag tctgtagccc ctggggaccg
     6721 atcacccagg tcgttgagcc gtcggtgaag gcagtgacat tgtctgcctt tctccagacc
     6781 atcaggccat taccagttcg ttggagcgag gcaccgctgt tggcatcgaa gtgctcgttc
     6841 tccagcggct gcccaactat gctggggatt aaggatgcga gggtcgagaa gccgaattgg
     6901 aactggggct gtgcttgggc ctcagttcct tccaccatgg ggagggctac ggccaatgtc
     6961 agggctaaag ctatcagcag acgcctcatt cctatgcctc catctgcctg tctgctacgc
     7021 acctcgaaca acggttggac ggcgcgggca gttgcaacgt gtatgccact atcccgacac
     7081 cccggcggtg cgactgcgga ccgcgcagta gggaaccggt atcggaagct aggcggtatt
     7141 gggcctggga gatcaggaac cgggcctggg gggacactgg gggaatggga gaggccggtc
     7201 cggatatctc caggccggcc tcgttaccaa cgagtaaccc tgtaccttac atcgttgctg
     7261 ctagagctgc gagtcgtctc cctgaacctt gctgatcatc tggaaggttg cgcccaggac
     7321 gaagagcacc gagatgatca ggagaccgag ggcgatcggc cgctggaaga agatgccgaa
     7381 gtcaccgcgg gagatctcca gcgactgcct gagacctcgc tccatcaacg gacccaggat
     7441 caaggtgagg cagatgggag ccagcgggaa gtccagcttc ttcagaatgt agccgacgac
     7501 gccgaaggcg agcatcacac ccacgtcgaa gacgctgctg tcgatgctgt aggagcccag
     7561 gacgcagaag cccaggatga tcgcgaacag gacggagtat ggaaccttca gcagcgccac
     7621 ccacaacggg atcagcggca ggttcaggat cagcaggatc aggttgccca cgtacatgga
     7681 ggcgatgacg gcccagacga agtcggcgtg ctccttgaag aggaaggggc cggggatcag
     7741 accgttcatc atgaaggcgc ccatcaggat ggcgatggtg ggcgagcccg ggatacccag
     7801 ggtgaacaga gggatcatgg cggcgttggc gtaggcgttg ttggccgtct cggggcccgc
     7861 cacaccctcg atcaagcctg tgccgaactt ctcggggtac ttggaggtct tcttctcggc
     7921 cacgtacgac atgaaggtcg ggaccacggc gccgacgccg gggatgaggc cgaggaagaa
     7981 gccgatgaag gtaccgcgga tgatcggcca gatggaggcc ttcaggtcct tccatgagat
     8041 gatcaaggag gacagagagg tctcgaagac atcggcgatg gggtgctcgg cgttggcgaa
     8101 gatctcgctg atgccgaaga ggcccatgac gacggggacg aagttgaagc cgtcgagcag
     8161 ctcggtctgg ccgaagttga agcgaggggc acccatgacg gggtcgatgc cgaccatggc
     8221 gatcatgagg ccgaagacgg cggccataag ggctcggacc atggacttgg aggcgaggcc
     8281 tgtgaccagg gagaggccca tgaccattag tgcgaagaac tcgggcgggc cgaacttcag
     8341 ggccagtgcg gtcagaggca gggccaggac gacgaggccg agggtggcga taccgccgcc
     8401 gatgaaggag ccgatggcag cgatggagag ggcggccccc gcgcgtcccc tcttggccat
     8461 ctcgtagccc tcgatgcagg tgatggccga ggcagcctcg cctgggacgt tcaccagcac
     8521 tgaggtgatg gttccgccgt acatggcgcc gtagtagatg gcggcgagca tgatgatggc
     8581 gggagtagga tcaaggacga aggtgacagg gatcagcatg gcagtgcctg cagccggacc
     8641 cacacctgga agcacaccga tcatggtgcc catgaggcaa cccatcaccg cgaagatcag
     8701 gttagtcggc tgcatcgcgg tggcgaagcc gttcatcaaa agaccgaagg tttccactta
     8761 accctcctat atcccgaaga tgccgatggg cagcgggacc ttcaggtact cggtgaacac
     8821 ctggaagact ccccagctac ccgcgatagc gacgattgcc gtcacgatca ggttctgccg
     8881 gccaagggca aagagcagga agaggtagaa ggccagcatt gtgagccgga agccgatcgt
     8941 ctccataaag aagacggcga aggagatcgc caggaagatg gccagcatcc gcaggtagcc
     9001 gaccttggag gcccagaagt cgtccggcat cggtccggcg ttcttgaatg tcgcgttgaa
     9061 gaacatcatt gccgccagga tcatcatgag gacggagagc cagaagggga agaaactggg
     9121 tccgggtccc agagcggtgt agaacttcag ttccattgaa tctctggcga tgaacgccgc
     9181 gaagaggatg aacaccactg ctgtgatctg ataaggacgt ttcataatcc accctttagt
     9241 ccatgatcga tggtttgccg gtacagggtt ttgggaatga gtcaacgcca atggtggaag
     9301 cacgactggc agctgtgcca gggatggttc ggcatctcgt cgcgccggtc aactatgacc
     9361 gtgatcggag cactgtctct gccgctgcca cgatattccg aggagtaatt aacgggttgc
     9421 tgcggattgc ccgcaccgga ggccagccag cagcaagccc gacatgatgc gcattacctg
     9481 accagggcgc aaatctcctt tctatgaaca aattgcgagg attatggtgt caaaggtagc
     9541 ttcaggtgcg tgtgtagaac cttagggaaa gcttaaggaa ggctgaggtt gagcttatgg
     9601 ggggtgtctg ttattttcag aatctcgcgg gcagttggag ccttgggcaa gatgcgcacc
     9661 ggtccagaac gactcgtcta tgcaggagtt gactcgcgca ggtatagtgc gcggaagcgg
     9721 tgaaccgggg gtgactcatc gtcctcgcac cgttcgaagg agaccaccct gcggtcggcg
     9781 tgcggaatgc gtccctcaga ataggccctc ggcgccggtg ccgagatacg agtgcttgat
     9841 gaggtcgctt tcggcgagat ccgtggcagg tccgtccaac acgatcttcc ccagttccat
     9901 cacgtatcct cggtcggcca ggtccaacgc catgctggcg ttctgctcca ccaacaggat
     9961 ggtcgtcccc ctggatcgga tctcgtcgat cacgt
//
LOCUS       DATALS010000282         9669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_162657, whole genome shotgun sequence.
ACCESSION   DATALS010000282 DATALS010000000
VERSION     DATALS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162657"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VHS06_06225"
     CDS             <1..456
                     /locus_tag="VHS06_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2987752.1"
                     /translation="VSTTAEQVHARHILVDSEDKAKEIVDKLKGGASFEDLAKSDSTD
                     TGTKDKGGDLGWFSREAMVQQFSDAAFKLEVNQVSDPVQTSFGWHVIQVLEKDPNRKL
                     DDQQLAQAKSNAVSKWLDTALQSSDVKRSLNESQKTWVYKRIKWTPPQS"
     gene            complement(510..1217)
                     /locus_tag="VHS06_06230"
     CDS             complement(510..1217)
                     /locus_tag="VHS06_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2987753.1"
                     /translation="MTAPRLVVLFDVDGTLIATEGRSRHSRAFTTAFEMVYGVPCRFT
                     SALHGMTDMQIFRHVAKSMGLDDDRGSELAREACRRMVEVYGVADPGDGRYIMLPGVR
                     ETLERLVAEGALLGLVTGNPPEIAEHKLSSVGVNDYFRFGAFGTEAEDRGELPPLAIR
                     RAEELIGESIPPQEVFVIGDTPRDIACAQESSCRSVAVATGMFAPADLEPLGPDLVLP
                     DLSDQSKLWRMMGIEDE"
     gene            complement(1510..2721)
                     /locus_tag="VHS06_06235"
     CDS             complement(1510..2721)
                     /locus_tag="VHS06_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987754.1"
                     /translation="MRTRPSYCVGLAISVLSALLLWSSLARAATTAGNPWSWGYNAYG
                     QLGDGTSELSNVPIQVGGVEDVIAISGGLAHTLTLKSDGTLWAWGNNSVGQVGNGGAS
                     DAPSGPTEVQGIDHVIGIGTGVEHSVALKSDGTVWAWGGNRYGQLGVGTTEDRALPVQ
                     VANLDRVTNIAAGGYRNLARRSDGTFWGWGTPGLGGAGFSSEAVRLSPVQIGGPAGVV
                     ELAVGIDHSMVVKDGMVYSWGLNKYGQLGDGTFDDHTGDLVAAKGLKDVVAVAAGSYH
                     SLALKKTGSLVAWGWNGSGQLGNGTTTDSNTPVQVKGLSGVVAMAAGWYHSIAVTQDG
                     SVWVWGDNLYGKLGDGSYKQSSAPRKLPGFAGVTGVGAGADYSLAVRVPPMPTPTAAS
                     PSTRTVTRRAR"
     gene            complement(2727..5084)
                     /locus_tag="VHS06_06240"
     CDS             complement(2727..5084)
                     /locus_tag="VHS06_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987755.1"
                     /translation="MGILAIATPALSGVASLVAAAQMGVAAWGSNSSYQLGTGSNASY
                     ANTPVTANTLELMTAIAAGGGHSLALRSDGTVWAWGSEERGQLGDGRPGIGFNSKTPV
                     QVMTDAVTPLTEVAAISAGEYHSLAVRGDGTVWSWGANDCGQLGYGTFEDSPYAQQVI
                     GFPPGTYISAIAAGQSHSMALDSVGNLWAWGFDGNGQLGNGPTLTPTVTPVPTETPGP
                     VTTATATNTPTPTRTPTPPATATPQLTPFATPVAINALPGVVAIGSGWAHSLAVRSDA
                     VWSWGANDYGQLGDGTKLRRDTPVQVMVGASALTGVTAVSGGRSHSLALKADGTVYAW
                     GRNSSGQLGDGSATDRTKPVRVVNLSRVTAIAAGQYHNLALSSDGTVWAWGDNAFGKL
                     GDGTLTRRTSPVKVVGFGTSNAAGAIAAGSNHSAAAPQPPTATSLPTSTPTQTSTPTQ
                     TSTPTETPTPTATGTTTITPTETVTPTETSTPTVTMTPTVTKTATPTRVSGTPGAAGS
                     TSGSRGASSPPPGPPSLSLPAVAGADQRYFSQTGYRVDQDAFWDYFQHRGGVRTFGYP
                     VSRTFTLLGCTVQVFQRGVVQMQPGGSVTVMNLLDQGLMPYTRINSSTFPAPDQELAQ
                     SAPSVYSANYADMALNFVRDNAPDYWNGLPVGFEQTFLNTVTYSDAFPNGGGEAGLLA
                     LMDLEMLGLPTSRPVPDPNNGDFVYQRFQRGILHYDRGTGQTQGLLLGDYFKAVLTGQ
                     GIPSDLDAEASGSPFYRQYNPGSPGSLARPDELPGSNLDGAFDPE"
     gene            complement(5255..5731)
                     /locus_tag="VHS06_06245"
     CDS             complement(5255..5731)
                     /locus_tag="VHS06_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX2987756.1"
                     /translation="MAETQVLDAEEQLVIFELANEVYGVDISRVQEIIRMTTITRLPR
                     APEFVEGVINLRGKVIPVVDLKRRFGLAEVDRTKASRIVVVDVGDHTIGMVVDAVSEV
                     LRVPSSAVEPPSPVVTTIESDYIRGIAKLEGRLIILLDLDKVLSWEEKRKLREVAA"
     gene            complement(5829..6536)
                     /locus_tag="VHS06_06250"
     CDS             complement(5829..6536)
                     /locus_tag="VHS06_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEX2987757.1"
                     /translation="MRKKIVAVLVLAVSGLAVFFYQSCGVGASGQGGITASGLGQTAP
                     KGTASPTKSPLRPTPTAVTPDEELSRALGKLTAKPEPGNAARETAVELPKRLRIPSIG
                     VDAGVEYVGLAADGAMDVPRDANHVAWYKLGPRPGAVGNAVIAGHVDWGGKVAVFWQL
                     GQLKPGDTIEVVAADDRKYEFVVQWQKWYDASTAPVQEVFGQAGVAEVTLISCGGEFD
                     KKSRQYLSRIVVRGILR"
     gene            6947..7363
                     /locus_tag="VHS06_06255"
     CDS             6947..7363
                     /locus_tag="VHS06_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987758.1"
                     /translation="MPDTPLGSAANGAGETDPPTAVVVDDQKGLAELLAERLSMDGFQ
                     ASVATSAKDALALCAQQDFDVAFIDLKLPDMNGLTAARQMRKLSAALKIILVTGFAVS
                     VDDQEVGSADINGVLPKPWRPAELEAILKTIGRRQP"
     gene            7360..8481
                     /locus_tag="VHS06_06260"
     CDS             7360..8481
                     /locus_tag="VHS06_06260"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX2987759.1"
                     /translation="MTHRRRLCPLPALLLLLAMVVLTTAQASANGVGTVYIACETDSS
                     ISVVDTTTNTVVESIPLAQPPLSLAFSSDAHFVYISHLNSNVISYLDTSIEDISATIK
                     VGKQVGQIVLTAGGTWLVATDPQEGVVRVIDPSARAELFAIPVGGQPSTIAASPDGRM
                     ALVADATSGQVSAVDLKSRSLLRTQRAAEGPHQISVSPSGQEFYVTSLEGNELLVFDA
                     ATGQLKRTLSAGQASAPVAFSTSGDVVYMPRKGKNEVAIIDGATGNTTGTIQLPAEAA
                     GFGANKSGRLAFASLKDASKLAVIDLTRRSVTVQVSQDKGPTFVAVSPLQPTTDNTVV
                     LPGALPNTGDPSTRIAASALLLGLLLTTVGLGLRAAGAR"
     gene            complement(8742..>9669)
                     /locus_tag="VHS06_06265"
     CDS             complement(8742..>9669)
                     /locus_tag="VHS06_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987760.1"
                     /translation="RGSGAASSVGQALGGALGVGVGTGVFPFLSPGHIPVLGLVAGAI
                     CGLSLASRSGPLARVTSGPELAVSLVEGVGWAAIAVAWCLLCGLGFQAALGGILRLAP
                     LWGGCFGWSVGCLLSIARNGAVPQMWRGRAEEIACAGAPMVVAGLGFAAALALALDLL
                     AVTAKVAPVAGYVQAKSSALLALPGSSITSALVAAVSCIGLVGGYELGLLTLLPAVPL
                     PRPLGASARKAGPVAKWCAELLLEMAVSHAGLNGPGHDVELKSRPVSTMGIVTRIHWT
                     SLLVTGITLMLGLYTVGNLLIALCAGVGIALG"
BASE COUNT         1894 a         3259 c         2682 g         1834 t
ORIGIN      
        1 gtttcgacca ccgccgagca ggttcacgcc cgccacatcc tcgtggacag cgaggacaag
       61 gccaaggaga tcgtcgacaa gttgaagggc ggggcaagct tcgaggatct cgccaagtcc
      121 gactccaccg acaccggcac caaggataag ggtggcgatc tcggatggtt ctctcgggag
      181 gccatggttc agcagttcag cgacgcggcc ttcaagctgg aagtcaatca ggttagcgat
      241 ccggtccaga caagcttcgg ctggcatgta atccaggtat tggagaagga tcccaaccga
      301 aagctggacg accagcagtt ggcgcaggcg aagagcaatg ccgtctccaa gtggctggat
      361 acggcactgc aatcgtccga cgtgaagcgc tccctcaacg agagtcagaa gacctgggtg
      421 tacaaacgga tcaagtggac tccgccacag tcgtagcaga tacggctaac agacacaagc
      481 gccggaggcg gaaacgtccc cggcgcttgt tattcgtcct caatccccat catacgccac
      541 agcttcgact gatcggacag atccggcaag acgaggtctg gccccagagg ctcaagatcg
      601 gcgggcgcaa acatcccggt ggccaccgca accgacctgc aactgctctc ctgggcgcac
      661 gcaatatctc gcggcgtgtc cccgatcacg aatacctcct ggggcgggat gctttccccg
      721 atcagttcct ccgccctgcg gatagcaagc ggcgggagtt ccccgcgatc ttcggcctcg
      781 gtcccaaaag ccccaaatcg aaagtagtcg ttcactccga cgctggatag cttgtgttcg
      841 gcgatctcgg gcgggtttcc ggtcaccagg cccaatagcg ccccctcagc caccagcctc
      901 tccagggtct cccttacgcc cggtaacatt atgtagcgtc catcgcctgg atcggccacg
      961 ccatacactt cgaccatccg acggcaggcc tctcgagcca gctcactgcc ccgatcatca
     1021 tccaagccca tggacttcgc cacgtgccgg aagatctgca tgtccgtcat cccatgcagt
     1081 gccgaagtga accgacatgg gaccccgtag accatctcaa aagcggtagt gaacgctctg
     1141 gagtgcctcg atcgtccctc cgttgctatg agcgttccgt ccacatcgaa caggacaacc
     1201 agtcggggtg cggtcaccta accctcccgt gcgccataca tcttgattct cttccccttg
     1261 ataatacgcg gtggccccag atcgaggcaa gaagagcgcc gctatcgatc cgcctcaatg
     1321 caaagaagcg acccccgatt ctggaggtcg ctcttctgtc tggagctcca ctagattaga
     1381 gccgatacct actgtcatgc cgaacgaagc gaggcatccc ctcgtgaacg gagagatcct
     1441 tcgctgtcgc tcaggttgac acaatcacag tggtcataca ctacagactc gcctgcagta
     1501 cgttgatccc tatcgcgctc gcctggtcac tgtcctcgta gacggagagg cagccgtcgg
     1561 tgtcggcata ggaggtaccc taactgcgag gctgtagtcc gcacccgctc caacacccgt
     1621 cacccccgca aacccgggga gcttacgagg agcggagctc tgcttgtagc tgccatcgcc
     1681 cagcttcccg tacaggttgt ccccccacac ccagaccgag ccatcctgcg taacggcaat
     1741 gctgtgatac catccggcgg ccatggcaac cacaccgctg agccccttta cctgaaccgg
     1801 agtattgctg tctgtggtgg tgccgtttcc caattgccct gagccgttcc atccccacgc
     1861 cacaagtgaa ccggtcttct ttagcgcaag gctgtgatag ctcccagcgg cgaccgcgac
     1921 aacatccttc agccccttcg ccgcaacaag atcgcccgtg tggtcatcaa aggttccatc
     1981 acctaattgt ccgtacttgt tcagccccca cgagtagacc atcccgtctt tcacaaccat
     2041 gctgtggtct atccccaccg ccagctctac cacgccggcc ggccccccta tctgaacggg
     2101 actcagcctg accgcctccg agctgaaccc agcgccgccg aggccgggtg tcccccaacc
     2161 ccagaaggta ccgtccgatc ttcgcgccag attccgatac cctcccgctg cgatgttcgt
     2221 gacgcggtcg aggtttgcga cctgcacggg cagtgccctg tcttctgtcg tacccacgcc
     2281 caactgcccg tatcggttcc ctccccaggc ccatacagtc ccatccgact tcaacgcaac
     2341 gctgtgctcc acgccggtac cgataccgat gacgtggtcg atgccctgca cctccgtcgg
     2401 gccgctcggc gcgtcgctcg ccccaccgtt tccgacctga ccgacgctgt tgttgcccca
     2461 ggcccacagc gtgccatcgg acttcagtgt cagcgtgtgg gcgagacctc ctgagatggc
     2521 aatcacgtcc tcgactccgc cgacctggat agggacgttg ctcaactcac tggtcccatc
     2581 ccccaactgg ccataggcgt tgtaccccca ggaccagggg ttacctgccg tcgtcgccgc
     2641 ccgcgcaagg gacgaccaca gcagcagggc gctcagcacg ctgatcgcca gcccaacaca
     2701 gtagctcggc ctcgttctca tcactgctac tccggatcga aagcgccatc caggttactg
     2761 ccgggcaact cgtccggcct cgccaggctt ccgggagacc ccgggttgta ctgtcggtag
     2821 aagggactac cgctcgcttc tgcatccagg tcagaaggaa taccctgacc tgtcagaacg
     2881 gccttaaaat agtcgcccag caagagccct tgcgtctgtc cagtccccct atcgtaatgc
     2941 aggatgccac gctggaagcg ctggtagacg aagtctccgt tgttggggtc aggaaccggg
     3001 cggctcgtcg gcagtccaag catctccagg tccatgagcg ccagaagtcc ggcctcccca
     3061 ccgccgttgg ggaatgcgtc gctataggtg acggtgttca ggaaggtttg ctcgaacccc
     3121 accggcaacc cattccaata gtccggggca ttgtccctca cgaagttcag tgccatgtcg
     3181 gcgtagttcg cgctgtacac ggacggcgcc gattgagcca actcttggtc tggggcaggg
     3241 aacgtgctgg aattgatcct cgtgtagggc atcagcccct gatcgagcag gttcatgacc
     3301 gtgacgcttc cgccgggctg catctgcact acccctcgct ggaagacctg aaccgtacag
     3361 cccaagagag tgaaggtgcg ggacacgggg taaccaaatg tccgtactcc gcctcgatgc
     3421 tggaagtagt cccagaatgc gtcctggtcc actctatagc cggtttggct aaagtagcgc
     3481 tggtcggccc ccgctacagc cggtaacgac agactgggag gcccgggcgg gggagaactc
     3541 gcaccacgag acccgctggt ggagccggct gcgcctggcg tccctgagac tcgcgtaggc
     3601 gtcgcggtct tcgtaaccgt tggggtcatg gtgacagtcg gagtcgaagt ctcggtgggc
     3661 gtcacagtct ccgtcggcgt aatcgtcgtc gtgccggtcg cggtgggggt cggggtctcc
     3721 gtcggcgtgc tcgtttgcgt gggcgtgctc gtctgggtag gcgtactcgt cggcaacgac
     3781 gtcgccgtcg gaggctgcgg cgctgctgcg ctatgattag acccagccgc gatggcacca
     3841 gccgcgttgc tggtgccgaa acccactacc ttgaccggcg acgtcctgcg tgtcagggtg
     3901 ccgtcgccca acttgccgaa ggcattgtcc ccccaggccc acacggtgcc gtcggatgac
     3961 agggccagat tatgatactg cccggcggct atggctgtga ccctggatag gtttaccacc
     4021 ctcacgggct tcgtccgatc cgtggcactg ccgtctccca actgcccgga gctgttccta
     4081 ccccaggcgt agaccgtgcc gtccgccttg agcgccagcg agtggctcct gccgcccgac
     4141 acggcggtca caccggtcag cgcacttgcc cctaccataa cctggacggg agtatctcgc
     4201 cgtagcttgg tcccatcgcc caactgtccg tagtcgttgg cgccccaact ccacacagcg
     4261 tcggacctga ccgcgaggct gtgggcccag cccgacccga tggcaaccac cccaggaaga
     4321 gcgttgatcg ccaccggcgt ggcaaaggga gtcaactgcg gagtagcggt agccggagga
     4381 gtgggtgtcc gtgtcggtgt aggcgtattg gtcgcggtag ctgtggtaac agggcccggc
     4441 gtttcagtcg gcacgggggt tacagtcggc gtgagagtcg gaccgttgcc cagttgtccg
     4501 ttgccgtcga aaccccatgc ccacaggttg cctacgctgt ccagcgccat cgaatgggac
     4561 tggcccgccg cgattgccga gatgtaggtg ccggggggaa acccgattac ctgctgggcg
     4621 tacggactgt cctcgaaggt accgtaaccg agttggccac aatcgttggc cccccaactc
     4681 cacacggtgc catcacccct gacggcgagc gaatggtact cgcctgcgct gattgcagcc
     4741 acctcggtca gtggcgtcac ggcgtcggtc atcacctgga ctggggtctt gctgttgaag
     4801 ccgatccccg gcctgccatc tcccagctgt ccccgctctt ccgaacccca cgcccagact
     4861 gttccatcac tgcgcaaagc cagagagtgc ccacccccgg cggcgatggc tgtcatgagc
     4921 tccaacgtgt tagccgtcac gggggtgttg gcgtagctag cgttagatcc agttcccaac
     4981 tggtacgaac tgttgctgcc ccacgctgcg acacccatct gagcggcagc cactagcgac
     5041 gccaccccgg acagagcagg agtggcgatc gcaagtatcc ccacacaaat caccagggtg
     5101 agggcatggc atctggactt tctaggcggc aacagcttca cgggagtagt ctccaacaac
     5161 ctgtatgtaa agggataaac cacagcaggc aggacggcgg ggagtgcgac cccttctccc
     5221 catgtccgtc ctgcctggct agtcaacgac tggactacgc cgcaacttcc ctcagcttcc
     5281 gcttctcttc ccaggagagt accttatcga ggtcgagcag tatgatcagc cggccctcga
     5341 gcttcgcgat accgcgaatg tagtccgact cgatggtcgt gaccacaggg gaaggaggct
     5401 cgacggcgct gctgggaacc ctcagcactt cggacacagc atccacgacc atgccgatgg
     5461 tatggtctcc aacgtccacc accacgatgc ggctggcctt ggttcggtcc acctcggcca
     5521 ggccaaacct cctcttgagg tcgaccaccg ggatcacctt gccgcgcagg ttgatgacac
     5581 cttccacgaa ctctggcgct cgcggcagtc tggtgatagt agtcatccgg atgatttcct
     5641 gtaccctgct tatgtctacc ccgtatacct cgttggccaa ctcaaaaatg actagctgct
     5701 cttcagcatc tagcacctgg gtttccgcca tccctctcgt ctcctttcac ccgccgctca
     5761 gactgtgcga gagctactac cattgataat cggcagattc gccaaatgct taaccctatt
     5821 cggcctgatt acctcagaat tccacggacc acgatccggc taagatactg ccggctcttc
     5881 ttgtcgaact cgccaccaca gctaatcagg gttacctccg ccacgcccgc ctgtccgaac
     5941 acctcctgca cgggagcagt cgatgcgtcg taccacttct gccactgcac gacgaactcg
     6001 tacttcctgt cgtcggcggc taccacctcg atcgtatccc ccggcttcag ttgccccaac
     6061 tgccagaaca ccgcgacctt gccgccccag tccacgtggc cggcaatcac cgcgttaccc
     6121 actgcgccag gtctcggccc tagcttgtac catgcgacgt ggttagcgtc ccgagggaca
     6181 tccatggcgc cgtcggcggc aagtcctaca tactcgactc ctgcgtccac gcctatggac
     6241 ggaattcgca gccgcttcgg caattcgacg gcagtctcgc gcgcggcatt gccgggctcc
     6301 ggcttcgccg tgagcttgcc caacgccctg ctcaactctt cgtccggtgt gacggccgtt
     6361 ggagtaggcc tcagggggct cttggtcggt gatgcggtgc cctttggcgc agtttgaccc
     6421 aaccctgagg cggtgatccc accttgcccg gaggccccca ccccacacga ctggtagaag
     6481 aagactgcca gtcccgatac ggcaagcacc aacaccgcaa cgattttctt tctcatggcg
     6541 gtctcccatc aaccccatta ctgcgtctag ataatatgaa ggaccgccgt ggtgcgtaac
     6601 ggaccaaagg ccccgaaacc ggccccattt cgtccctctt tcgatggccc agccggcccg
     6661 gaaaacacgc gcaccacgca atgacttatg cggtcaaacg ccactcgtcg cgccggcagt
     6721 tcttcaactt tcgcgtcacc gcgcaagttg acgactattc cggcatgacc tagaatccgc
     6781 tatagcttga caggggggat gtgtggcact gaaccatcac ggcctctctt ccgcgctctc
     6841 tccgtctgag catattcctc gcaccgcagt tgccgtccgg ttccttatcc tggccggcac
     6901 acccctccaa tttcgatcga tttcgatgtg tctgaggtga ctgaaattgc cagatacccc
     6961 tttggggtct gcggcaaacg gcgctggtga gacagatcct cccaccgccg tagtcgtaga
     7021 cgaccagaaa gggctcgccg agttgctggc tgaacgtctg tccatggacg gcttccaagc
     7081 cagcgtcgcc accagcgcga aggatgccct ggcgctttgt gcccagcagg acttcgacgt
     7141 tgccttcatc gatctcaaac ttccggacat gaacgggcta accgccgcaa gacaaatgag
     7201 gaaactatct gcagccctca agatcattct cgttacgggc ttcgccgtct cagtagacga
     7261 ccaggaggtg ggatccgcag acatcaacgg cgttcttccc aaaccctggc gtccggcgga
     7321 gctggaagca atcctgaaga cgattgggag gcgtcaacca tgacacatcg acgacgactg
     7381 tgcccactac ccgccttgct gctcctcttg gccatggtcg tgctcactac cgcccaggct
     7441 tcggccaacg gggtgggcac ggtgtacatc gcgtgtgaga cagacagctc catttcggtc
     7501 gtcgacacga caaccaatac tgtcgtcgag tcaatccctt tggctcaacc tcccctatct
     7561 cttgccttca gttctgatgc ccacttcgtc tatatcagcc acctgaactc caacgtcatc
     7621 tcttacttgg acaccagcat cgaagatata tcggccacaa tcaaggtcgg caagcaggta
     7681 gggcagatcg tgctaacagc gggtggcaca tggctcgtgg ccacggatcc tcaggagggc
     7741 gttgtcaggg tcatcgatcc gtccgcccgg gcggagttgt ttgccattcc ggttggcggt
     7801 cagccctcaa ccatagccgc gtctcccgat gggcgaatgg cacttgtcgc cgacgcgact
     7861 tcagggcagg tctccgcggt cgatctcaag tccagatcgc ttctgcgcac ccagcgagca
     7921 gccgaaggcc cgcatcagat atcggtttcc cccagcggcc aggagttcta cgtgacatcc
     7981 ttggaaggga acgagctact ggttttcgac gccgccaccg gacaactcaa acgcaccctc
     8041 agcgcgggac aggcctcggc tccagttgcc ttctccactt cgggtgatgt ggtctatatg
     8101 cctcgcaagg gtaagaacga ggttgccatc attgacggtg caaccggcaa caccaccggc
     8161 accatccagc ttcccgctga ggcggcgggc ttcggtgcaa ataagagcgg ccgtcttgcc
     8221 ttcgcatccc tcaaagacgc gagcaagttg gcggtaatcg acctcacacg gcggtcggtc
     8281 acagtacagg tgagtcagga caaggggccg accttcgtcg cggtctctcc acttcagccc
     8341 actactgaca acacggtggt cttgcctgga gcgctcccca acaccggcga tccttccacc
     8401 cgcattgcgg catcggctct actgctgggt ctgctgctga caacggttgg gttgggcctc
     8461 agggcagccg gcgcgaggta gtgcgtcatt caacagtaag gggctcggca ttagtgccgg
     8521 tccacaagac tagtcctctt acacgctgat cttgacacta gaagttgcat agtgcccctt
     8581 acggggcagt atcgcttaca tgaacagtag ggcgggtcag gggcgacccg ccctaccaga
     8641 tcgaaaagca agcactactc atagatcagt cgagcgtgac gctgcgctcc tagaaatcac
     8701 gactccacct tcggcaccct gccctactgg ttgaagtgcc cttagcccaa ggcaataccg
     8761 acaccggcgc acagggcgat cagcaagttt cccactgtgt acaggccgag catcagggtg
     8821 attccggtga ccagaaggct cgtccaatgg atcctcgtga cgatccccat agtgctcacc
     8881 ggcctgctct tcaactccac gtcgtgaccg ggtccgttca gtccggcatg gctcactgcc
     8941 atctccaaga gcaattccgc gcaccacttg gccacaggac cggctttcct cgcgctcgcg
     9001 cccaggggac gcggaagcgg gaccgccgga agaagcgtga gtaggcccag ctcatacccg
     9061 cccaccaacc cgatacagga tacagccgct accagggccg atgtgatgct ggatcctggc
     9121 agcgccaata gagcagacga cttcgcctgc acatatcccg cgactggcgc gactttcgcc
     9181 gtcacggcaa gcagatcaag cgcgagcgca agagcagcgg caaaccctaa acctgcaaca
     9241 accatcggtg cgccggcgca agcgatctcc tcagcccgcc ctctccacat ctgagggacg
     9301 gcgccgttgc gggcgatcga gaggagacag cccacagacc acccgaagca tcccccccag
     9361 aggggcgcga gccggagtat gcccccaagc gccgcttgga agccaagtcc gcacaagagg
     9421 caccaggcca ctgcaatggc tgcccagccc acaccttcca ctagagacac cgccagttca
     9481 ggcccagacg ttacccgagc cagggggcct gatctggatg ccaggctcag cccgcagatc
     9541 gcccctgcaa ctagtccaag cacaggaatg tgacctggcg agaggaacgg aaagacgccg
     9601 gtgccaaccc caacgcccag cgcccccccc aatgcctgcc caacagatga tgctgctcca
     9661 gaaccgcgt
//
LOCUS       DATALS010000283         3728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_58324,
            whole genome shotgun sequence.
ACCESSION   DATALS010000283 DATALS010000000
VERSION     DATALS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58324"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VHS06_06270"
     CDS             <1..272
                     /locus_tag="VHS06_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX2987761.1"
                     /translation="GGFETSLMMALQPELVREDHIVSFRPPDRGPFFSAPLLEEPKVT
                     LVRPRRPGGTGLGGDPSLGARDKGERLLEMAVASVAEFVVYYASA"
     gene            324..2471
                     /locus_tag="VHS06_06275"
     CDS             324..2471
                     /locus_tag="VHS06_06275"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX2987762.1"
                     /translation="MAAEVAGGPKVLFPYGAMYYRRPNPPPEDWERDFQTMQAHGFNM
                     VKCWAMWNVMHTSEGEFDFSEFDGLLEVAARHGIKVVINTILDSTAPHWLWRTHPESW
                     YQSQDGRRRHPVSDGHNVTGGARGLCLDNDPVWEQGQEFLRAIARRYKDHPSLWGYDV
                     WNEVNSREDPPGPEALYYCYCEATSAKFRDSLRQKYGSLAALEAAWHHHFRTWDEVYP
                     PYGRGAFPDNMDWQDFKVADRVRLLRERVQVLRSEDPVHPMISHGIGGVSRKLYDYMG
                     DEWFWASEVDEWGITVAPGRRDNDPRNFFQMVDLVRSAARGKRIWLGELQGGHMFLTP
                     ELEGRPAGDARIATAGDISMWNWITLMTGAKGVLYWRYRPERVGPLTDFFGLCERDGS
                     PTPRLEMAGRFARLTGGHPEIVEAQPVRGDMAILVLHESQRYANVAHARLFHAWADAI
                     RGIYQACIDHGIQADMVELSDIGEYPLVYLPYPLMVLKENAARLREYVENGGCLVSEA
                     GLASYSDLGFACPQLPGLGLDQVFGTEAEIVHLMPDILQDLKLNINGGAIQANGVTIQ
                     GRVGRQVLRPTTGRAIASFPDGSIAGVENSLGQGKAMLVGSYPSVAYRESQDRATGAW
                     MAGLIEWAGQEPRLRTSAPASVARLHRRGEATYLYVLNTSHDEVQASVSLAGPSGAFR
                     QGTSMEDSRVFPFQGNKIRVPLPAQDGMILRLD"
     gene            3416..3673
                     /locus_tag="VHS06_06280"
     CDS             3416..3673
                     /locus_tag="VHS06_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987763.1"
                     /translation="MNARWFVALTLIVALATLLPGVAGAAPTPAPTPTSTPFATVGSG
                     GQNSSKTPTVATAATAPATATPVTATSTPAPTVKPNNQAGD"
BASE COUNT          702 a         1201 c         1189 g          636 t
ORIGIN      
        1 cggggggatt cgagacctcg ctgatgatgg cgctgcagcc ggagctggtg cgggaagacc
       61 acatcgtttc cttccggccc ccagaccggg gtcccttctt ctctgccccg ctgctagagg
      121 agccgaaagt gaccctggta cgcccgcggc ggcccggggg caccgggctg ggcggcgacc
      181 cctcgctggg agccagggac aagggtgagc gcctgctgga gatggccgtg gccagcgtgg
      241 ccgagttcgt ggtgtactac gccagcgctt aaactactgc ttgcgatttg gcagtacctt
      301 caccaggcga tgaggcatcg catttggctg cagaagtagc cggaggaccg aaagtgctat
      361 tcccctacgg tgccatgtac taccggcgtc ccaaccctcc gccagaggac tgggaacgcg
      421 actttcagac catgcaggcg cacggcttca acatggtcaa gtgctgggcg atgtggaatg
      481 tcatgcacac ctcggagggc gagttcgatt tctcggagtt cgacggcctg ctcgaagtcg
      541 ccgcccgcca cgggatcaag gtggtgatca acaccatcct ggattcgacg gcccctcact
      601 ggctgtggcg cacccatccg gagtcctggt atcagtccca ggacgggcgg cgtcgccatc
      661 cagtttccga tggccacaat gttacaggcg gcgccagggg gctgtgcctg gacaacgacc
      721 cggtctggga gcaggggcag gagtttctgc gggccatcgc ccgcaggtac aaagaccacc
      781 cgtccctctg gggctacgat gtctggaacg aagtgaactc ccgcgaggac ccgcccgggc
      841 cggaagccct ctactactgc tactgcgagg ccaccagcgc caagttccgg gactctctcc
      901 gccagaagta tgggtcgctg gcggctctgg aagctgcctg gcaccatcat ttccgcacct
      961 gggacgaggt ctacccgccc tacgggcgag gtgccttccc ggacaatatg gactggcagg
     1021 acttcaaagt ggctgacagg gtgcggctgc tgcgggagcg cgtccaggtg ctgcgctccg
     1081 aggacccggt ccatcctatg atctcgcacg gtatcggcgg cgtttctcgg aagctgtatg
     1141 actatatggg ggatgagtgg ttctgggcca gcgaggtcga cgaatggggg attacggtgg
     1201 cccccggccg ccgggacaac gacccccgca acttcttcca gatggtggac ctggtgcgat
     1261 ctgcagcccg aggcaagcgc atctggctgg gagagctgca gggcggccac atgtttctga
     1321 cgcctgagct ggaaggccgg ccggctggcg atgcccgcat agccaccgcg ggcgacatca
     1381 gcatgtggaa ctggataacg ctgatgaccg gcgccaaagg ggtgctctac tggcgttacc
     1441 ggccagagag agtgggcccg ctcaccgact tctttggcct ctgcgagcgg gatggcagcc
     1501 ccacgccccg cctggagatg gccggccgct tcgcccgcct taccggcggc catcctgaga
     1561 tcgtcgaggc gcagccggta cgcggagaca tggccattct ggtcctgcac gagagccagc
     1621 gatatgccaa cgtggcccac gcccgcctct tccacgcctg ggccgatgct atcaggggga
     1681 tctaccaggc ctgcatcgac catggcatcc aggccgacat ggtggagctg agcgatatcg
     1741 gcgaataccc gttggtctac ctgccatacc ccttgatggt cctgaaagag aacgcggcga
     1801 ggctgagaga gtatgtagaa aatggtggct gcctggtatc cgaggccggg ctcgcctcct
     1861 acagcgacct gggcttcgcc tgcccgcagc tgccgggcct gggcctggac caggtattcg
     1921 ggaccgaggc cgagattgtc cacctgatgc cagacatcct ccaagacctg aagctcaaca
     1981 ttaatggggg ggctatccaa gctaacgggg taactatcca aggaagggta ggccggcagg
     2041 tactgaggcc cacgaccggc cgcgccatcg cctctttccc ggacggctcc attgcgggag
     2101 tcgagaacag cctcggacag ggcaaggcta tgctggtagg cagctatccc agcgtcgcct
     2161 accgggagag ccaggacagg gctacggggg cgtggatggc cgggttgatc gagtgggccg
     2221 gccaggagcc gcgcctgcgg accagcgctc cggcgagcgt agctcggctg caccgccgcg
     2281 gcgaagccac ttacctgtat gtgttgaaca ccagccatga tgaggtacag gcaagcgtct
     2341 ccctagctgg ccccagcgga gcgttccggc agggcaccag catggaggac agccgggtgt
     2401 tcccattcca gggcaataag atccgggtgc cgctccccgc ccaggacggg atgatcttga
     2461 ggctggattg acatatttca acttcatctg gctcgaaaat taggcgcagg taacggctca
     2521 cccccgccga ggggattggc ggggcctgtg gcgggcgcca cggctggcgc ccgactcagc
     2581 ccatgttaat gtgcgttcat gtggctgtta atgtgagcgt gagataatga tcgcgggtct
     2641 ggacctctca ggctcagttt ccgtccgagg ccaggctggt cgcgccccag cctggccatc
     2701 tcgttatccc tggcgcagag acatctccgt tcccaggcta cgctagatta cccccggttg
     2761 ccctgcggca acctggcgaa cggcccggcc ggcacccacc ctacaagagc accccttttc
     2821 caccccggcc cccgaccggc ccggcggacc ccgtccaggc ccatcacctt ggttcaggca
     2881 gacacgaatg cgggcccgag cttcccctgc acacccccgc acccatagca cgttgccttt
     2941 gcctgcagac ggtagcggaa ggcgattttg tcagcattaa cagcccggta aaagcatttg
     3001 ttacgcacct ctaaccgcat ttggcagaga aatcagttgt tcagcaaaaa aacccgtgtt
     3061 acaatttagc acagctaaac tagctgggga gcgttcggag cggcctgcaa ggagcccgcc
     3121 ggtaaccccc ccggagatcg gcaggggcaa atcatccccc acagccaagt caggtacttg
     3181 gaccacctag tcaacgacct agccgaatac aggtacgctg atggaggctg tgatccgagg
     3241 cgaccgaggc gccctgtgat agggtgcgcc ccgcattggt cgctggtgcc ctcggagagc
     3301 cagtagcgaa accggccatc agctcgaatg ggccggtttt ttttggcccg tggttgtatg
     3361 cgggtggcac cggcgcccac ccaggaacct ggcgaggaca agggaggcaa agtaaatgaa
     3421 cgcaagatgg ttcgtagccc tgacgttgat cgtcgctctg gcgacgctgc tgcccggcgt
     3481 agccggggcc gcacccacgc cagcgcccac ccctaccagc acgccctttg ccactgtggg
     3541 gtctggtggc caaaacagct ccaagacgcc caccgtggcc acggccgcca cagcgccggc
     3601 tacggctacc ccggtaacgg ccacgagcac cccggcacct accgttaaac ccaacaacca
     3661 ggccggcgac taagctacgg ccaccgccac cacgggtact acggctacag cgatgcccac
     3721 gggtacca
//
LOCUS       DATALS010000284         5443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_211355, whole genome shotgun sequence.
ACCESSION   DATALS010000284 DATALS010000000
VERSION     DATALS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211355"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VHS06_06285"
     CDS             <1..245
                     /locus_tag="VHS06_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987764.1"
                     /translation="PEDVDALADASGPLNKLSPDQLADRLRRFGLAEEDARGYRDAVL
                     RGGVFIAVSTPKGAESAAQEILADADATRVRLLAVP"
     gene            complement(645..1097)
                     /locus_tag="VHS06_06290"
     CDS             complement(645..1097)
                     /locus_tag="VHS06_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987765.1"
                     /translation="MPSSGRVQRTRFNVRWTPAPLSVAERRAESTMQPMAIGLFPSPT
                     EASLGLDNLSEAEFGPSMISLVMKRPEDVDALADASGPLNKLSPDQLADRLRRFGLAE
                     EDARGYRDAVLHLSLLKGDGTRAHLLVEIVLYPILLVVTRARLRRLRP"
     gene            1292..3181
                     /locus_tag="VHS06_06295"
     CDS             1292..3181
                     /locus_tag="VHS06_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987766.1"
                     /translation="MRRHSLLICLALAAVLLLGTSASAEMLSPGDLDYDIPNGHFFTQ
                     TNGRPLGTDSTGYAVTNDDGVPFWDAFQRYGGKDVLGYPVTKRFMYDGFVTQAMQKVV
                     FQWRPDVKQVWFLNTFDALQDKGKNDWLLVYRQTPKPFDTSPDNGLSWDQVVARHLAL
                     LDQNQPIKDRYFSDPQYIDHFGLPVSTADMGNSFVIRAQRATFQYWKEDVPWAKAGDV
                     SVANGGDLAKEAGLWPADALTLSTPDGRPAGQPAAALQAAATPKPAATPQPANFPPYN
                     PTPGLYRAASPEYGIHSFVFSHPDTAVRTFRQVTDLGFGWNKSLVEWKWVEPLKKGEF
                     DWYATDGVVYGSAGQGLKLVVRVDLPPAWATKKNPGQPESPPDYLQDYADFVYALVSR
                     YNNSNPRGRIYAIEVWNEPNLADYWGGKKPNAAEYVQMLKVAYEAAKRADPSVVVLSA
                     GLGPTGTNNDSAIPDDVFLQQMYDAGARSYFDVLGAHAPGFKAPPEMSGDQVAADPAY
                     GGHRVFTFRRVEDLRNVMVKNGDSGKQVWITEFGWTSDTVHPAYAWHRVTEEQKGQYL
                     VRAMQWAKNNWAPWIGVMCVWNLPSQDWTNAREELWWSIANPDGSPRTAYTMIQQARR
                     SGVLP"
     gene            complement(3419..4840)
                     /locus_tag="VHS06_06300"
     CDS             complement(3419..4840)
                     /locus_tag="VHS06_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX2987767.1"
                     /translation="MKAGLQWIEEKTPDLISLASAIWEYAEPSLWEERSSQLLADYLA
                     QEGFEVRRGVGGMPTAFVASWGSGRPIIGYLGEYDALPGLSQRAIPRREPLVPGAPGH
                     GCGHNLLGVGSLGAAVALKRELQAHGTAGTVRYYGCPAEETAIGKVFMAKEGVFDDLD
                     VALSWHPNAINMPNSGTSLALNSAKFAFHGRSSHASAAPHLGVSALDAVELMNVGVNF
                     LREHVPPETRIHYVITNGGSEPNVVPALAEVWYYVRAPHRVEVESVYQRIVKIAEGAA
                     LMTGAELEIKFQTGLYDLLPNNVLSDLLEESLRTVGVPSFTEEEREFARGIEDSFTLG
                     QKAAMLRTRHLPQEFLDITLHDGVAWDFDRGETMWGSTDVGDVSWIVPTGQITTACYV
                     VGTGGHSWQAAAASGTSIGHKGMITAARALGFAGYQLVSRPEFLAQAREAFEKSRGDK
                     RYQSPLPQELNAPLAQLPDHGYH"
     gene            complement(4837..5391)
                     /locus_tag="VHS06_06305"
     CDS             complement(4837..5391)
                     /locus_tag="VHS06_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2987768.1"
                     /translation="MEGGFQFTVNTDVDREAKFQERPDLTLRNRLKAENPGLEAQKRI
                     IRAADRKWTGNTQTLVDASNGFGDKAVACFLRRIKPGESSDIHRHNFEALGYVVKGRG
                     YEIHDGERIDWAEGDALFIPANVWHQHFNSDPKEDAVLLLITNWPLLLHLGICTLEPA
                     PTWEDALSRPSAIPDPVLKKVEAK"
BASE COUNT         1001 a         1832 c         1641 g          969 t
ORIGIN      
        1 gcccggagga tgtcgatgcg cttgccgacg cctcgggccc gctgaacaag ctgtcgccgg
       61 accaattggc cgataggctt cgccgattcg ggttggcgga ggaggatgcg cgtggttacc
      121 gggacgctgt tctgcgcggc ggcgtcttca ttgccgtgtc tacgcctaaa ggggccgagt
      181 ccgctgctca ggagatcctg gccgacgccg atgccacccg agttcggctg ctggctgttc
      241 cttaggagtg cgtagagatg cttttccaca gctatcgacc ggcacttgct ctgctcgccg
      301 ccctcctcct tctctcaccg ctctctgcat gtagctctca aggggctacc ggcccttctg
      361 ccagccctac cgctggtgtc cagatcggtc agcggacaaa gacctccggg tgccaggcaa
      421 acggacccct gccggacaac gcctgtacgc cgggggcaat cttcgcctcc gtcacgaaag
      481 accaggtttg tcagtccggc tacgcgaaga gtgtcaggga tgtgccagat tcggagaaga
      541 accaggttta cgcggagtac gggatcacct ctcacaatcc gggggagtac gaggtggacc
      601 acctgatcag cctggagctg ggagggtcca acgacatagc caacctatgg ccggaggcgg
      661 cggagccgcg cccgggttac cacgagaagg atagggtaga gaactatctc caccagcaga
      721 tgtgctcggg tgccatctcc cttcaagagg ctcaagtgca gaacagcgtc ccggtaacca
      781 cgcgcatcct cctccgccaa cccgaatcgg cgaagcctat cggccaattg gtccggcgac
      841 agcttgttca gcgggcccga ggcgtcggca agcgcatcga catcctccgg gcgcttcatc
      901 accagcgaga tcatcgacgg gccgaactcg gcctccgaga ggttatccag tcccagggac
      961 gcctcggtgg ggctcggaaa cagtcctatg gccatcggct gcatggtcga ctccgcgcgg
     1021 cgctccgcca ccgataacgg cgcgggcgtc catctcacat tgaaccgagt ccgctgcacg
     1081 cgcccactgg acggcaagac caactaccac ctggcaggtt ttgtgcccac cgcaccttcg
     1141 ctgtgtccga tcgcggccga tacggccaac cccgtcacaa caagtatgtt gcaatccaac
     1201 actacgcttg tataatctgc tcaccttgcc tctacgcggg ccgatacccc ttccgccctt
     1261 caccgacaac tgttccgacg gaggactacc cttgcgcagg cactctctac tcatctgcct
     1321 agccctggca gcggtgctgc tgctaggcac ctccgccagc gcggagatgc tctcgcccgg
     1381 agacctggac tacgacatcc ccaacggtca cttcttcact cagaccaacg gccggccact
     1441 ggggacagac tccaccggct acgccgtcac caacgacgac ggcgtgccct tctgggatgc
     1501 tttccagagg tacggtggca aggacgtgct gggctatccc gtcaccaagc ggttcatgta
     1561 cgacggcttc gtcacccagg ccatgcagaa ggtggtcttc cagtggcggc cggacgtgaa
     1621 gcaggtctgg ttcctgaaca ccttcgacgc cctccaggac aagggcaaga acgattggct
     1681 gcttgtgtat cgccagaccc ccaaaccctt cgacacttcc ccggacaacg gtcttagctg
     1741 ggaccaggtg gtggcgcggc acctggcgct gctggatcag aaccagccca tcaaggatcg
     1801 ctacttctcc gatccccagt acatcgacca cttcgggctg cccgtttcca cggccgacat
     1861 gggcaactcc ttcgtgatcc gagcccagcg agccaccttc cagtactgga aggaagacgt
     1921 gccgtgggcc aaggcgggtg acgtgagcgt ggccaacggc ggcgacctgg ccaaggaagc
     1981 cgggctgtgg cccgccgacg cgttgaccct ctccacgccg gatggccgcc ctgccggcca
     2041 gcctgccgcg gcgctccagg cggctgccac gcccaagccc gcggccacgc cgcaaccggc
     2101 caacttccca ccatacaatc ccacccccgg cctctacagg gccgccagcc cggagtacgg
     2161 gatccacagc ttcgtgttca gccacccgga cactgccgtc cgaaccttca ggcaggtcac
     2221 ggacctcggc ttcggctgga ataagagcct cgtcgagtgg aagtgggtgg agccgctgaa
     2281 gaagggagag ttcgactggt acgccacgga tggagtggtg tacggatcgg cgggccaggg
     2341 gctgaagctg gtcgtcaggg tcgatctccc gcccgcctgg gccaccaaga agaaccccgg
     2401 ccagccggag agcccacccg actacctcca ggactacgcc gacttcgtat acgccctggt
     2461 cagccgatac aacaacagca accccagggg gcggatctat gccatcgagg tgtggaacga
     2521 gcccaacctg gccgactact ggggaggcaa gaagcccaac gctgcagagt acgttcagat
     2581 gctgaaggtg gcctacgagg cggccaagcg ggcggatccc agcgtcgtgg tgctctccgc
     2641 gggcctcggc cccacgggca cgaacaacga cagcgcaatc ccggacgacg tcttcctgca
     2701 gcagatgtac gatgccggcg cccgcagcta cttcgacgtc ctgggcgccc acgcgcccgg
     2761 cttcaaggcc cccccggaga tgagcgggga ccaggtggcg gcggatcctg cctacggcgg
     2821 ccatcgggtc ttcaccttcc ggcgggtcga ggatctacgg aacgtcatgg tgaagaacgg
     2881 cgactccggc aagcaggttt ggataaccga gttcggctgg accagcgaca ccgtccaccc
     2941 ggcctatgcg tggcacaggg tcaccgagga gcagaagggg cagtacctcg tccgggcgat
     3001 gcagtgggcc aagaacaact gggcaccctg gatcggggtg atgtgcgtct ggaacctccc
     3061 ctcgcaggat tggaccaatg ccagagagga actctggtgg tccatagcca acccggacgg
     3121 aagcccgcgt accgcctata ccatgatcca gcaggcacgg cggagcggag tactgccctg
     3181 acggagtgct gagtgctggg tgctgagtgc tgaggatgag aggccggggt cgttaccgac
     3241 cccggccgtt cgtacggatg acctgacgcc aaagacggca gctacaggca cgccagcacc
     3301 gactcctcca cggtgatcca cccagggcag gggcgtctca gctggacagc cgacctccat
     3361 gcagatgatg gtgcgcagcg gtgtcggatc caagatccgc gggtcctctt tcgtatgttc
     3421 aatggtagcc atggtcgggc agctgggcga gcggggcgtt caactcctgc ggcaacggag
     3481 actggtaccg cttatcgccc ctggacttct cgaatgcctc ccgcgcctgc gctagaaact
     3541 ccggcctgct taccagctga taaccggcaa accccagggc tcgtgccgcc gttatcatgc
     3601 ctttgtggcc aatgctggtt cccgatgcgg cggcggcctg ccacgagtgg ccgccggttc
     3661 ccaccacgta acaagccgtc gtgatctggc ccgtgggcac gatccagctg acgtcgccaa
     3721 catccgtcga tccccacatc gtctctcccc tgtcgaaatc ccaggcaacg ccgtcgtgca
     3781 gcgttatgtc caggaactcc tgagggagat gtcgggtccg aagcatcgcg gccttctgcc
     3841 ctagagtgaa ggagtcctcg atacccctgg cgaactccct ttcctcctct gtgaaggatg
     3901 gaacccccac cgtccgtagg ctctcctcca ggagatcgga tagaacgttg ttgggcaaca
     3961 ggtcgtacaa ccctgtctgg aacttgatct ctagctcggc accggtcatg agggcggctc
     4021 cctccgcgat cttgacgatg cgctggtaga cgctctccac ctccactctg tgtggcgccc
     4081 gcacgtagta ccacacctcg gccagggccg gcacgacgtt aggttcgctc cccccgttgg
     4141 ttatcacgta gtggattctg gtctcggggg gcacgtgctc ccgaaggaag ttcacgccga
     4201 cgttcatcag ctcgacggca tccagcgcgc tgaccccgag atggggagcc gcggaggcgt
     4261 gggaactcct gccgtgaaat gcgaacttcg ccgagttgag tgccaacgaa gtgccgctat
     4321 tcggcatgtt gatggcgttg ggatgccacg agagggcgac atccaggtcg tcgaacaccc
     4381 cctccttcgc catgaatacc ttgccgatgg ccgtttcctc ggccgggcag ccgtagtagc
     4441 gaaccgttcc tgccgtcccg tgcgcctgaa gctccctctt gagcgcaacg gcggcaccaa
     4501 ggctccctac tcccagcagg ttgtggccgc acccgtggcc cggggctccg gggacaagcg
     4561 gctccctacg aggaatcgcc cgctgggaca acccggggag ggcgtcgtac tccccgagat
     4621 agccgatgat cggccggccc gagccccagg acgccacgaa ggcggtgggc atgcccccga
     4681 cgccacgcct gacctcgaac ccctcctgcg cgaggtagtc ggccagcaat tgcgacgatc
     4741 gctcttccca caggctcggc tccgcgtact cccagatggc acttgccagc gagatcaggt
     4801 cgggagtctt ctcttcgatc cactgcaacc ccgccttcat tttgcctcca ccttcttgag
     4861 aacagggtcc ggtatcgccg aaggacggct cagcgcatcc tcccaggtgg gtgccggctc
     4921 cagggtgcag atgcccaggt ggagcaagag tggccagttg gttatcagca gaaggacggc
     4981 atcctccttc ggatcggagt tgaagtgctg gtgccagacg ttggcgggga tgaacagcgc
     5041 atctccctcc gcccagtcga tcctctcgcc gtcgtggatc tcgtatcccc gacccttcac
     5101 cacgtagccc agcgcctcaa agttgtggcg atgtatatcc gagctctccc ctggcttgat
     5161 tcgccgaagg aagcaggcaa cggccttgtc gccgaagccg ttggaggcat ctaccagggt
     5221 ctgtgtgttg cccgtccact tgcgatcggc cgccctgatg atcctcttct gcgcttccag
     5281 ccccgggttc tccgccttga gccgattgcg gagcgtcaga tcgggacgtt cctggaactt
     5341 cgcctcgcga tctacatcgg tattgactgt gaactgaaaa ccaccttcca tgattacctc
     5401 tccttatcta acctaatctg cgcgccacgc cagggcccgc ttc
//
LOCUS       DATALS010000285         3777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_283700, whole genome shotgun sequence.
ACCESSION   DATALS010000285 DATALS010000000
VERSION     DATALS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283700"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1494)
                     /gene="hisD"
                     /locus_tag="VHS06_06310"
     CDS             complement(88..1494)
                     /gene="hisD"
                     /locus_tag="VHS06_06310"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX2987769.1"
                     /translation="MINLRTARSLQEGRRLLLAHRGLDELSAPPGLAARIQTTFGEPL
                     TPGQVVERIVTAVKAEGDVALERYCRLIDGSFPRPLKVDRSEMEAARRAISPELLSAF
                     QTSAERILAFHRKALRQSWFETSPSGSYGQIVRPLSRVGLYVPGGTAAYPSSLLMSAL
                     PAKAAGVQDVIVTTPPARDGSVPAVVLAAADVAGVSAVYRVGGAQAIAAMAYGTETIP
                     GVEKILGPGNLFVALAKRMVAGTVGIDAITGPTETLIVADDSTSLEHVAADLLAQAEH
                     DVLAQAVLITTSSALLDGLPAELERQLAGLSRASIARESMEGRGVAVLVSTLDEALEL
                     ANEYAPEHLCLEVEEPWSVLDSVRAAGGVFLGTRSIEALGDYVAGPSHVMPTGGSARF
                     SSPLTVDDFLKVSSLFAVSEEGLRTLGPAAVLMARSEGLDAHAASVQRRLDERIRRGV
                     DATSPDPSANANSADEGM"
     gene            1719..2633
                     /locus_tag="VHS06_06315"
     CDS             1719..2633
                     /locus_tag="VHS06_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987770.1"
                     /translation="MVDNGRGFGLAEVAGRHSGNQGLISTPERADLIDARLHVMTAPG
                     RGTRVSLVVPVKPDQNTTHPLESPMPKKTPAQNNIRILVVDDHPLFRQGVRGALDAEP
                     DMEVVGEAADGEQALTMAAELLPDVVLSDVNIPGPDGVEITRSLKGVLPRTPVILMTA
                     YDDEEQLFDAIKVGAAAYFLKDLAPNELMDGIRRVSRGEYLINENVLSKPMVASRVLK
                     QFTDFSPDEKEVEPLFVPLSNREIEVLDQIAKGNSNKEIARALKISDQTVKNHITSIL
                     RKLAVNDRTQAVVYAIRHGWIKMEELPK"
     gene            2638..3732
                     /locus_tag="VHS06_06320"
     CDS             2638..3732
                     /locus_tag="VHS06_06320"
                     /inference="COORDINATES: protein motif:HMM:NF017221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2987771.1"
                     /translation="MTDEANSNLQTRSPLGDALDSFRQEIASMAEQSKEISLLIKQTA
                     DEIDRLTQINREQSSQMRQLQANIDAYPRTEIQQRYTTWQEAQMRLFMQQNQMEQLRN
                     RQANLERSQQMAMGLLEVVDSLTGSMEAGLAITSGSQLMGQGNDSGGQGPVTALIQAT
                     ELVYHRLSRELQDSAAQMLSDLILRAEICERLVDRDPSMAKGEISQLRQATAQALKSV
                     RKLIQALEPPALKELGLPTALRRYVSAEMVGESPRVLLEINGKERRLPHVEEIGVFRI
                     LQEGLTNAVQHSGGECIKLTLHFETDRVTAQLSDDGHGFDVATALKQATTRIRSGLVE
                     MRVRADSIGASLEINSYPGRGCTINLALPA"
BASE COUNT          827 a         1162 c         1118 g          670 t
ORIGIN      
        1 gcggggcaca agaccccgcc ctacatctat tcatccgacc tagatgcctt cgtccgccgg
       61 ttctccaccc aacggatcgg atgggcccta catcccttcg tccgccgaat ttgcattggc
      121 ggatggatcc ggtgaagtgg catcgacacc ccggcgaatc ctctcgtcca agcgtcgctg
      181 gacagaggca gcgtgggcat cgagtccctc ggaccttgcc atgaggacgg ccgcggggcc
      241 gagggtgcgc agcccttctt cgctaacggc aaagaggctg ctcaccttca ggaagtcatc
      301 cacggtgagt ggcgatgaga agcgggcgct gccgccggtg ggcatcacat ggctgggacc
      361 ggccacgtag tcgcccagcg cctcgatgga tcgcgtgccc aggaacacgc cccctgccgc
      421 ccgcacggaa tccagcacag accagggctc ctccacctcc aggcagagat gctccggggc
      481 atactcgttc gccagttcca gtgcctcgtc cagggtcgat accagcacgg caacccctcg
      541 accctccatg gattccctgg cgatggaggc gcgactcagt ccggccaact gccgctccaa
      601 ctcagcgggt agcccatcca gcagtgccga agaggtggtg ataagaacgg cctgcgccaa
      661 cacatcgtgc tccgcctgcg caagtaggtc cgccgcgacg tgctcaagag aggtagaatc
      721 gtctgcaacg atcagcgtct cggtcgggcc ggtgatcgca tcgatcccca cggtccctgc
      781 aaccatccgc ttggccagtg ccacgaagag gttacccggg ccaaggatct tctcgacccc
      841 gggaatcgtc tctgttccat aggccattgc ggctattgcc tgagcaccac cgaccctgta
      901 aaccgcagag acgcctgcaa catccgcggc tgcaagcacc accgccggta cactcccgtc
      961 cctggcgggc ggcgtcgtca cgatcacgtc ctgaacgccc gcggccttag cagggagtgc
     1021 gctcatcaat agcgagctgg gataggcagc tgttccaccc ggcacgtaca gtccgacccg
     1081 agagagagga cggacgattt ggccatagct tccggacgga gaggtctcga accaggattg
     1141 tcgcagcgcc ttgcggtgga acgccagtat tcgctccgcc gacgtctgga aagcagagag
     1201 cagctccggg gagatagccc gccgagccgc ctccatctcc gagcggtcga ctttgagcgg
     1261 cctgggaaag ctgccgtcta tcagccgaca gtagcgctcc agggcaacat cgccctcagc
     1321 tttcacagcc gtgacgatcc gctcgactac ctggccgggg gttaggggtt caccgaaggt
     1381 agtctggatg cgagcggcta gccctggggg cgccgataac tcgtcgagac ctcggtgagc
     1441 cagcaggagc cgacggccct cctgcaaaga tcgtgcggtg cgcaggttta tcatcgtaag
     1501 ctctcaagat gcaagcttca ctgatcaaga atggccatta gccaacaact tacacgttag
     1561 gttgaaaggt gatggtcaat ggagcacaac tttgcgcgat tgtagcactt tgtacagaaa
     1621 aggtgtagcg tgccttgccg gtgatctcag ccgaatgcta tacttttcgc taggagtagc
     1681 tagtcttcga tatcgaccca gttgtgcttc agctctctat ggtagacaat ggtcgcggct
     1741 tcggcttggc agaggtcgcc ggccggcact caggaaatca gggactgatt agcacgccgg
     1801 agcgggcgga tctgatagac gctcgactcc atgtcatgac tgctcctggc aggggaacca
     1861 gggtctcact ggttgtcccg gtgaagccag atcagaatac cactcaccct ctcgaatcgc
     1921 cgatgccgaa gaagacgcct gcccagaaca acatacgtat cctcgtcgtg gatgaccacc
     1981 ccctcttcag acagggggtg cgcggagcgc tggacgccga gcccgacatg gaagtggtgg
     2041 gagaggccgc ggatggcgaa caggcgctga ccatggccgc ggaactgctg ccggacgtgg
     2101 tgctcagcga tgtgaacatc cccgggccgg atggcgtcga gatcacccgt tcattgaagg
     2161 gggtgttgcc tcgcaccccg gtgattctca tgaccgccta cgacgatgaa gagcaactct
     2221 tcgacgccat caaggtaggc gcggccgcct attttctcaa ggatcttgca cccaatgaac
     2281 tgatggacgg aatccgccgc gtcagcaggg gtgagtacct catcaacgag aacgttctct
     2341 ccaagccgat ggtagcgtcc agagtgctga agcagttcac agacttctct ccagatgaga
     2401 aagaggtgga gcccttattc gttccactct ccaatcgcga gatcgaagtg ctggaccaga
     2461 tcgccaaggg caacagcaac aaggagatcg cgcgggcgct gaagatcagc gaccagacgg
     2521 tcaagaacca catcacttcc atcctccgca agcttgccgt gaacgatcgc acccaggcgg
     2581 ttgtgtacgc catacggcac gggtggatta agatggaaga actgccgaag tgatgcgatg
     2641 accgacgaag ctaactccaa ccttcaaacg cggagcccgc tgggagatgc tctggactca
     2701 ttccgccagg agatcgcctc catggcggag cagagtaagg agatatctct tctgatcaag
     2761 cagacggcgg acgagatcga tcgcctcacg cagatcaacc gtgaacaatc ctcccagatg
     2821 cgccagctcc aggccaacat cgacgcctac ccacgcaccg agatccagca acggtatact
     2881 acgtggcaag aagctcagat gcggctcttc atgcagcaga accagatgga gcagctgagg
     2941 aaccggcaag ccaatctcga acgaagccag cagatggcca tggggcttct tgaagtggtg
     3001 gactccctga ctgggagcat ggaagcaggt cttgccatca cctctggcag ccagcttatg
     3061 gggcaaggca atgactcggg aggacagggg ccggtgactg ccctgatcca ggcgacagaa
     3121 ctggtctacc accgcctatc tcgagagttg caggatagcg ctgcccagat gctgagcgac
     3181 ctgatcctgc gagcagagat ctgtgaacgg ctggtggaca gggacccttc catggccaaa
     3241 ggagagatct cccaactgag gcaggcgacg gcccaagcat tgaaatcggt caggaagcta
     3301 attcaggcgc tggaacctcc tgcgttgaag gaactcgggc tgccgaccgc cctccgacgc
     3361 tatgtgtctg cggagatggt gggagagagt ccaagggtgc tcctagaaat caacggcaag
     3421 gagcggaggc tacctcacgt tgaggagatc ggggtgttcc gtatcctcca agagggcctc
     3481 accaacgcgg tacagcactc cggtggggag tgcatcaagc tgactcttca cttcgagact
     3541 gaccgggtga ccgcgcaact ctcagatgac ggccacggct tcgacgtagc aaccgccctg
     3601 aagcaagcca caaccagaat acgttccgga ttggtcgaga tgcgcgtgcg cgccgacagt
     3661 atcggcgcat cgctggagat taactcctac cccggccggg gttgcacaat caatctagca
     3721 ctccccgcct gacccctctt ctcatcagat cccgccaata actagtacga tcctcct
//
LOCUS       DATALS010000286         7756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_58550,
            whole genome shotgun sequence.
ACCESSION   DATALS010000286 DATALS010000000
VERSION     DATALS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58550"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..718)
                     /locus_tag="VHS06_06325"
     CDS             complement(26..718)
                     /locus_tag="VHS06_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987772.1"
                     /translation="MDSFDSDTNLTTLHAVRGDLERPAAEGARLLDALVVAFTQKADE
                     MGQRLVRRLSELERSLLANREVLKLWMADDMVQHLFPAIDEARRLLAAEEYGPLKALL
                     DGLEKQVAERSTQAQELEEKQQRRLYLLKAVRQVCAEMGFCEISLPHYQREGDRGSPI
                     SFSVDTLDRGRIQFTLSLEAISSFSEIADDRCFEEFSELSRYLSEQFGISTRSSVSTL
                     KLIGLPRSPSRW"
     gene            complement(960..1328)
                     /locus_tag="VHS06_06330"
     CDS             complement(960..1328)
                     /locus_tag="VHS06_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987773.1"
                     /translation="MAIDRGSKVTFYRCRLCGESHGMVDVREVLCVLDYRMTEKQEMV
                     DGSLRINWHKHGGMFDFDRIEIIRATDEDVMRLAMDAGNDIDPHRFPHYAGMPCYIDP
                     ECSLSENVRRILQDRFGKSC"
     gene            complement(2510..5767)
                     /locus_tag="VHS06_06335"
     CDS             complement(2510..5767)
                     /locus_tag="VHS06_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987774.1"
                     /translation="MPNTKPLNPTRAIWQAWRNTSDAVLAGKLLPLVSSLPIGQALDR
                     LFAAISMTYGAVSGLTFGILAANLPLADDYLHVSWPQAAAFAAVGAFCSGVWRVVRHR
                     RRGRLPFWGEILGMFVPPNSVSFWLPGAALAVILLGLRLRGNAGDLVVGVGCPLTWWY
                     IRVLLDHRSYLCRMPVTHTCGWFTLFTLGCWWAGLPCRFALIALLFALPHVVVTQALI
                     RRYRQDGSLWRHRSWFFWWRTWPDPLTLEQAIRMQCTGNPEYASAFAEHLAYIDAHLN
                     EPRPVTESVSALRGWESTRAGVWPQEDYVSWLPKQYWIERTVEGRALLSMLGGDAQSE
                     IYGRYRQAVRVWSWAERTRACHALLRADGAAVGPLLAQLQERDPELRQTAAHLLRRIA
                     ANTSPHFAADTAGLFCPRCLTVFHANRIRLGRTETITIHGCRSCYQRESAIVRPQQII
                     AVLDEHMDNDAECVDGTLRANAFVRDGSFDFDAVEIIGASHQQIERFVRLIADDKDAL
                     RRANYRGVRVLVRDTCRLTEYSLWLLKKVLGEVVIEGGGAAACEVGEDRCLRPMKAWR
                     RSRWRRVLWCQWRYTSDASFAAELFETIHQGPAHWLATCVLLLLGALGGSIACIVVGI
                     PAANWNIRAARLVVPWSHVGHLARLGAFAGTAVAAWQRCHAGRRISWGLFLEWVVPWL
                     DVRGDPRPGTNDELRVGLLQGFLFGTAFALLAQCVCACVIILVRVSADGAYMPVHRLS
                     IGNTGVYVLVFGLAFGVFGALGFGPIRALAAGMVGVFFGLLFAGMVGGIVGGLALGLG
                     VEVLCVLDRVFSQPCGSGCTGDDALPHRHVCLWWRRQPDLLTLEYALREACLADGHAR
                     LRWTKPLAALSIEPASARPADEIILSLQHRDWMEQIRAANELLAIGGEAIEPLLAAST
                     GEGRELRRNAAHMIRAIAEDTTRHLVPGIATLLCSRCLVGVASRRVGCGPGRKVSVYG
                     CRACGQSHSFIERPHQITVVLDDQMSANQEMIGGILRVNWLCRGEVFDFNHVEVLHAT
                     DELVLRFAVAVGNDTDPYRLARYPRVHCHVSPGCALSDNSLRILAARFGRVSATP"
     gene            complement(5917..6534)
                     /locus_tag="VHS06_06340"
     CDS             complement(5917..6534)
                     /locus_tag="VHS06_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX2987775.1"
                     /translation="MQLGVDRTGFPLLRLPLTGIEMHALPITKVQFERFLADPNEYGD
                     SWYEAVLAVNPRVAPYAFSAENRERLLLTGILPFEVEAFCAWLGEGFRVPTAEEWRAA
                     YNELGSIPREAVAGLQDLPAIAAGILHRLAAQIPTGSAREFALLAGGVVEWVRRGDSW
                     LGLGAPRYQFHRNAWNPLFNEVRPIRAGDRLPFFGFRLVRGGGAG"
     gene            complement(6566..>7756)
                     /locus_tag="VHS06_06345"
     CDS             complement(6566..>7756)
                     /locus_tag="VHS06_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2987776.1"
                     /translation="HPTPRRRYASRRTLRLVVLLLLTGTIYRIIHHNHLNALKVKERQ
                     AAQSDEHARQWAAAIEHYNHLLDSDPNDPDSRARRAQAYERLGEWNAALADFDRLVKV
                     RPQDRDIWLGLARADWALGHYDKSVQSYTKTLAIAPHDTIALNERGMIHAARARWKDA
                     AADLVEAARLEIDNPADAKTGEERGWTTAQLCFRAAAMQLATGDQAGYRRLCVTLFAH
                     LDQIEAAGAARGPGSPASNQSRRASVLGDAALTFVLAPGALPDYTRLLRSLSGLVASD
                     PRNSVNRILYGAALCRARQYAAAIAELDEGSRLSGTTGTILDRPFLALAHRGLGHSRK
                     AARYAADTARLEAQIRRAQANASPPLALEVDVACQVLAREVGPLSLDEANVLARDTGR
                     PPSR"
BASE COUNT         1635 a         2496 c         2275 g         1350 t
ORIGIN      
        1 gcttctgtga gatcagcctg ccgcattacc aacgggaagg tgatcgcggt agcccaatca
       61 gcttcagcgt tgacacgctt gatcgggtgc tgatcccgaa ttgctcgctg aggtaacgtg
      121 atagctccga gaactcctca aaacagcggt cgtcggcgat ctcggagaaa ctactgatcg
      181 cctccaacga gagtgtgaat tggatccgcc cccgatcaag cgtgtcaacg ctgaagctga
      241 ttgggctacc gcgatcacct tcccgttggt aatgcggcag gctgatctca cagaagccca
      301 tctcggcgca tacctgccgc acggccttca gtagatagag acgcctctgc tgcttctcct
      361 ccagctcctg agcctgagtg cttctttccg cgacctgctt ctccagtcca tccaggagtg
      421 ctttgagagg cccgtactcc tcggcggcaa ggagtcgacg cgcctcgtcg atagcaggaa
      481 agagatgctg caccatatca tccgccatcc atagcttgag cacctcccga tttgccagga
      541 gagaccgctc caattcggat agccgccgca caaggcgctg tcccatctca tccgccttct
      601 gcgtgaaggc gaccaccagg gcgtccagta ggcgggcacc ttccgcggcc gggcgctcca
      661 agtcaccgcg caccgcgtgc agcgtcgtca agttagtgtc actgtcgaat gaatccaccg
      721 tagggatccc acccgaccta agccacgatt cggcagcggc cacttctgca tggaaggtag
      781 cgcgcaatcc tggcgatgcg ccagcaagca tagcatccat gcgctcgcgc agacccagga
      841 tctcggcgtg aagatcgcgc aatctgccta caagacgtaa cttctcctca cgctctctct
      901 gtaagcgata ttgcgagcat ttgataccac tcatccggca cctttcctcc ccttgtcaat
      961 caacaggatt tcccgaacct atcctgcaag atcctccgca cgttctctga gagagaacac
     1021 tcgggatcaa tgtagcaggg catcccagcg tagtggggaa atcgatgggg atcgatgtca
     1081 ttcccagcat ccatggccaa gcgcattacg tcttcgtcag tcgcacggat gatctcaatg
     1141 cggtcgaagt caaacatgcc tccgtgcttg tgccagttga tccgcaggga gccatctacc
     1201 atctcctgct tttcggtcat tcggtagtcc agcacacaca agacctcccg tacgtccacc
     1261 atgccgtggc tttcgccgca cagccgacag cgatagaacg tcaccttcga cccgcgatcg
     1321 atcgccatga catatgggct gcaggtggcc aggcagcgtg agcagaagag gtgcatccca
     1381 cttgcatcta gccgagccag tgtgttccgg ctgatttgct ctagtaggga agcacccgtc
     1441 cgccggagtg cagggctcca atgggtgatc atctgctgta aggccggtac tgcatcctct
     1501 cccatattga caagcgtgtg gcatacccta gctacctcga tccagtccca cgcgggccga
     1561 tcgcccacac cggccgtcca gggtcgtcct acaaagcgga gtgaatgcag gcagtgcaac
     1621 agagggggca cattccggct gccaagcggc tcggctgcgc gcaggaggaa cctgtctaag
     1681 ggagcatttc tgataggatc ctgatgcacg agaacggcca gtgcctcttc cacatcgatt
     1741 gggtgcggcg ctagaggcca ccaccaacag tggtcgcgta actcgtgcag tcccgcaccg
     1801 cgccgtgacg ttgcaccccc gggcagaagg gcggctccag ctacggcgaa gaagagaacc
     1861 cccatgccgt tgccgccaag aggtatcagc ccgcacgtgg cgcaagtaag caggagaagc
     1921 cggagctgga actcgactcg cggggcgcgc aacggcgcca gcagcagaac acccagcagt
     1981 cccaagcaga tgctgatggc tgccccgggt cctggtgccg ttactgccac caccacgaat
     2041 gcaacaacac ctgcgcccaa gccgcgtagg aggagcggaa cgatgccagc aggccgaacg
     2101 accccttctg gcagccagcg tctaatcgaa agcagagcaa gcaaccagga ttcccagcgt
     2161 atccgccgca ggcccaacgc ataggctgca gccatcgtta gcgcgcctac tgccatccct
     2221 aggaaagggt agggcccggg gccgccccac ggcgacgtag acgcctggcg ctgacagaca
     2281 agaaccaccc accatacgac gacaccgaga gccgctccat aggctaaggc gagtggtagc
     2341 aggtagatca gtatgccggc gcccgccggt tcgtccatca ggtggatctc cagctgcgcc
     2401 acgaaatcgg tatcggacgt gtgcttccat gcctgccgga gaacagctct ccaacggtgc
     2461 ttggcatctc tttgtcgacc actggtaaga tgcatggtcc tacacctgct cagggagttg
     2521 cgctcaccct tccgaacctc gccgccaaga tcctcaggct gttatccgag agcgcgcacc
     2581 ccggcgagac atggcaatgc accctcggat agcgggccaa gcggtaggga tccgtatcat
     2641 tgcccacggc aacagcaaag cgcaacacca attcgtccgt tgcatggagt acctcaacat
     2701 ggttgaaatc gaacacctca ccgcgacaca gccagttcac acgcaagatg ccaccaatca
     2761 tctcctgatt cgcagacatc tggtcgtcta gcacgacggt gatctggtgg gggcgctcta
     2821 tgaaggaatg gctctgcccg caggcacgac acccgtagac ggatacctta cgcccgggtc
     2881 cgcacccaac ccggcgtgat gccaccccca ccaggcagcg ggagcagagc agcgtagcga
     2941 tgcctggcac taggtgccgc gtagtatcct cagcgatggc gcggatcata tgggccgcgt
     3001 tccgccgcaa ctcccgaccc tcgccggtcg atgccgctag cagtggctcg atcgcttcac
     3061 caccaatggc cagaagctcg ttagcagccc taatctgctc catccagtcg cggtgctgca
     3121 gcgagaggat gatctcgtcg gcagggcgag ctgatgcagg ttcgatggaa agggcagcca
     3181 acggcttcgt ccagcggagc cgtgcgtgcc catccgcgag gcaggcctcg cgcaaggcat
     3241 actctagcgt cagcagatcc ggctgccggc gccaccatag gcacacatgg cggtgtggaa
     3301 gggcatcatc cccggtgcat cctgagccgc aaggctgtga aaacacccga tccaaaacgc
     3361 atagaacctc aactcccaag cctaatgcaa gcccgcccac gatcccaccg accatccccg
     3421 cgaacagcag gccgaagaag acgccaacca tccccgcagc gagggcgcgg attggaccga
     3481 acccgagagc gccgaacacg ccgaaggcaa ggccgaacac cagaacgtat actcccgtgt
     3541 tcccgatgga aaggcggtga accggcatat aggcgccatc ggcgctgaca cgaaccagga
     3601 tgatgacaca ggcacagaca cactgagcaa ggagcgcgaa ggcagtaccg aagaggaatc
     3661 cttgcaggag cccgacacgc aactcgtcgt tcgtgcctgg gcgtgggtcg cctcgcacat
     3721 ccagccaagg gaccacccat tccagaaaaa ggccccagga aatccgcctt ccagcatggc
     3781 agcgttgcca cgcggcaaca gccgtcccgg cgaaggcacc gagccttgcc aggtggccca
     3841 cgtgtgacca cggcacaact aggcgtgccg cccgaatgtt ccagtttgcc gcagggatgc
     3901 cgactactat gcaggcgatg gatccaccca gggcacccaa cagtaacaag acacacgtgg
     3961 caagccagtg ggcaggtcct tggtggatcg tctcgaacaa ctcggcggca aacgacgcgt
     4021 cggatgtata ccgccactgg caccagagca ctctacgcca ccgagagcgg cgccacgctt
     4081 tcattggcct cagacagcgg tcttccccca cttcgcacgc cgcagcaccg ccgccctcga
     4141 tcactacttc gcctaacacc ttcttcagaa gccacaggct atactctgtc aggcggcacg
     4201 tgtccctcac cagaacccgt acaccccggt agttggctcg gcgtagcgca tccttgtcat
     4261 ctgcgataag cctgacgaag cgttctatct gttgatggct tgcgccgatg atctcgacag
     4321 cgtcgaaatc gaaggatcca tcgcgaacga aggcgttcgc ccgcagtgtg ccatccacgc
     4381 attcggcgtc gttatccata tgctcgtcca ggactgcgat gatctgctgc gggcggacaa
     4441 tcgccgattc gcgctggtag caactgcggc agccgtgaat ggtaatggtc tcggtccgcc
     4501 ctagccgaat acggttggca tggaagacgg tgagacagcg cgggcaaaaa agccctgccg
     4561 tgtcggctgc aaagtgcgga gaagtgttgg cggcgatacg gcgcagaagg tgcgcggccg
     4621 tctgccgcaa ctcgggatct cgttcctgga gttgggctag cagcggccca acagcagcgc
     4681 catctgcgcg gagaagcgca tggcacgcgc gggtcctctc tgcccaggac cacacccgaa
     4741 ccgcttggcg gtaacggccg tatatctccg actgggcatc gccgccgagc atggagagga
     4801 gtgccctccc ttccaccgtt cgctctatcc agtactgttt gggtagccaa gacacgtagt
     4861 cctcttgagg ccacacgcca gccctggtag attcccatcc ccggagtgcg gagacagatt
     4921 ccgtgactgg gcgcggttcg ttgaggtgag catcgatgta ggcaaggtgc tcggcgaagg
     4981 ctgaggcata ctcaggattc ccagtgcatt gcatgcggat cgcttgctcg agtgtaagtg
     5041 ggtcgggcca ggtgcgccac cagaagaacc atgaacggtg gcgccatagc gagccgtctt
     5101 ggcgatatcg tcggatcagg gcctgcgtaa cgacgacgtg aggaagagcg aacaacaaag
     5161 cgatgagagc aaagcggcac ggcaaaccgg cccaccagca tcccaatgtg aacagtgtga
     5221 accagccgca ggtgtgggta actggcattc ggcacagata cgagcggtga tcaagaagca
     5281 cccgtatgta ccaccatgtg agagggcatc caacccccac cacgaggtcc ccagcgtttc
     5341 ctctgagcct gagcccaaga aggatgacgg caagtgctgc tcccggaagc cagaaagaga
     5401 cgctgtttgg aggaacgaac atgccaagga tctcgcccca gaaaggcagc ctgccacgcc
     5461 gccgatggcg gacgacacgc cacactccgc tgcagaacgc cccaacggcc gcaaaggccg
     5521 ccgcctgcgg ccaagacacg tgcagatagt cgtcggccag ggggaggttt gcggccaata
     5581 tgccgaaagt cagtccggaa accgcaccgt aggtcatcga gatggctgcg aagagccggt
     5641 cgagtgcttg cccaatcggc aggctggata ccagtggcaa gagcttgccg gccagcacag
     5701 catcggacgt attccgccag gcctgccaga tggcgcgagt cgggttcagc ggtttcgtgt
     5761 tcggcaagag gaatgctggc tgaggggggt gcccctgggg ggcggatgga gttccggtcc
     5821 aacgcctagt ccgccgacgt ggcggccccc ccggaattcg tgggatggca gaccaaccat
     5881 cccgccgact cggcggagat ctgcgacgcc gtcgtatcat ccggcccctc cacctcgcac
     5941 cagccgaaac ccaaagaacg gcaggcgatc accagcacgg atcggcctga cctcgttaaa
     6001 cagggggttc cacgcgtttc gatggaactg gtagcgcggc gcacccaagc ccagccagga
     6061 atccccacgc cggacccact caaccacccc tcccgccaga agagcgaact cccttgcgga
     6121 tccggtcggt atctgggcag ccagccggtg tagaatccct gcagcaatcg ctggcagatc
     6181 ttgcagaccg gctacggcct cgcgtgggat gcttcccagt tcgttgtagg ccgcgcgcca
     6241 ctcttctgcc gtcggcacgc gaaatccctc acccagccaa gcacagaacg cctccacctc
     6301 aaatggtagg atgcccgtca acaggagtcg ctctcggttc tccgcgctga aagcgtaggg
     6361 tgcaacacgc gggttcacag caagcacggc ctcataccag gagtccccat actcgttcgg
     6421 atctgccagg aaccgttcga actgcacctt ggtgattgga agcgcatgca tctcgattcc
     6481 ggtgagcggc agtcttagca gagggaatcc cgttctatcc acgcccaatt gcatgtcgta
     6541 cctccatcgg cccgtcgtct gcatatcagc gggagggtgg ccggcctgta tccctcgcca
     6601 gcacgtttgc ctcatccagg ctaagcggcc ctacctcacg tgcaaggacc tggcatgcca
     6661 cgtctacctc cagtgcgagg gggggcgatg cgttcgcctg tgcccggcgg atttgtgcct
     6721 ccaagcgtgc tgtgtcggcg gcgtagcgcg ctgcttttcg gctatgtccc aggcctcgat
     6781 gcgccagcgc caagaagggc cgatccaaga tggtgccggt ggttcccgac agccgggatc
     6841 cttcgtctag ctccgcgatg gcggcggcgt actggcgagc acggcagagt gctgcgccgt
     6901 agaggatcct gtttacgctg ttcctgggat cacttgcgac cagtcctgag agcgaccgca
     6961 gcaggcgtgt gtagtcaggc aacgccccag gggccaggac gaaggttaag gcagcgtccc
     7021 ccagaacgga tgctcggcgc gactggttcg atgctggaga tccggggccg cgagccgcac
     7081 cagcagcctc gatctggtca agatgtgcga acaacgtcac acataggcgg cggtagccgg
     7141 cctgatcccc tgtggcaagc tgcatggccg cagcacgaaa acagagttgc gccgttgtcc
     7201 atccccgctc ttcgccagtc ttggcatccg ccggattgtc gatctccaat cgagcggctt
     7261 ctaccaaatc ggcggcagcg tccttccaac gcgcacgggc agcgtgaatc atgccacgct
     7321 cgttcagcgc gattgtgtca tgcggggcaa tcgccagcgt cttcgtgtaa gattgcaccg
     7381 acttgtcata gtgccccagc gcccagtcag ctcgggccag acccaaccag atgtctcggt
     7441 cctgtggccg caccttaacc agtctatcga agtcggcaag tgcggcgttc cactccccta
     7501 ggcgttcgta tgcttgtgcg cggcgcgcac ggctatccgg atcgttgggg tcactgtcaa
     7561 gcagatggtt gtaatgctca atcgcagctg cccactggcg cgcatgctcg tcagactggg
     7621 cagcctgtcg ctccttcact ttgagagcat tcagatgatt gtgatgaata attcggtaga
     7681 tggtgccagt cagaagcagc agcaccacca accggagggt acgccgagat gcgtatcgtc
     7741 gccgtggggt ggggtg
//
LOCUS       DATALS010000287         7212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_163317, whole genome shotgun sequence.
ACCESSION   DATALS010000287 DATALS010000000
VERSION     DATALS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163317"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1241
                     /locus_tag="VHS06_06350"
                     /pseudo
     CDS             65..1241
                     /locus_tag="VHS06_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
     gene            1244..2407
                     /locus_tag="VHS06_06355"
     CDS             1244..2407
                     /locus_tag="VHS06_06355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2987777.1"
                     /translation="MDFELTPEQKSLQEEAQAFAAAEFDPAYAMRCEQDRRYPSDLRQ
                     KAGTRGLLAVYIPREYGGRGLGLLESVLVIEAMCRKDPGLGMALSLASLGTPVTLRYG
                     SEEQKRTILPRVAAGEIMTGIALTEPGHGSDLTDVATTATRTSSGYVVEGRKTLISNG
                     QNARYVTLLCKTDPEARPPHRGLSLILLDTNRPGYKASDLGLKMGMRMMSTAEISLQG
                     VKVPASNLIGMENRGFYHTLEFLNESRIEVAAQALGDAEAAFDRALARVKERRQFGKR
                     LADLQVTQHKIADMATKIETARLLTYMAAWQSDQGGSDPVQASMAKLHAARVAVEVAE
                     EAIQLFGGYGYFLENEVERIYRDARVTEIYEGTREIQKNTIARYLLGSWREEA"
     gene            2413..3609
                     /locus_tag="VHS06_06360"
     CDS             2413..3609
                     /locus_tag="VHS06_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX2987778.1"
                     /translation="MAGRVGIVGAAVTPFHSKWYEKTYYELAQMATAEALKDAGLSPE
                     EVDGVFYGIYNDIFQRTCIPEHPLQGLIGMQNKFGIRISNGGATGAYTLSAAHAYVAA
                     GRFKAALVLGVEKSTDCFDYQSMSATPEVIKTIGWSGDSFFEQKLGWTASDSYAEVVL
                     AYMDEHPDDLKPEITAQVSALLSRHVKGNSFAQRQHDQVTPEEVMNSRIVVYPFKELE
                     ICVYSEGAAALIVAEEDTAKAISRNTGNPVIWISGVGESNEHSFAGKNQKVMHRIMSD
                     HLAAYRAYEMAGVKDPLKEIDVVEAHDAFVPQLEITMAEMGFAPLGRADSLIEEGIVT
                     PGGPIILNPSGGLIYCGHAVGASNIMSAWSARKELINRGLRRALVHGTGSTVAQYGVA
                     FVLEHD"
     gene            3623..4144
                     /locus_tag="VHS06_06365"
     CDS             3623..4144
                     /locus_tag="VHS06_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010979867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987779.1"
                     /translation="MAKKLIPTEAIPEQGGEIVSIDGKGYLMLNDAMFTFYRRSMGEL
                     TPFFLALRDEKKILGARCTNCGIVRVPPFATRCPDCNFAPTELVEVGDVGRMLNTPPI
                     TYFANALFQQQVPFGRGRVVLEGADTAISVNIYTSKGILVPGLITKDTEVKVVFRKDR
                     TGEITDLFCPSVT"
     gene            complement(4126..4227)
                     /locus_tag="VHS06_06370"
                     /pseudo
     CDS             complement(4126..4227)
                     /locus_tag="VHS06_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
     gene            complement(4238..5218)
                     /locus_tag="VHS06_06375"
     CDS             complement(4238..5218)
                     /locus_tag="VHS06_06375"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX2987780.1"
                     /translation="MAKMTMVQALNLALRQEMEKDDRVIILGEDIGKDGGVFRVTEGL
                     VEQFGEDRVVDTPLAESGIVGTSIGMAVYGLRPVCEIQFAGFDYLNFSQIESHASRLR
                     HRSQGRHTVPMVLRAPYGAGVRALEHHSESREAFWSHIPGLKMVIPSGPRNARALLVS
                     AIRDPDPVIFYEPKAVYRAFREEVPEAEETLPLGKSQVVREGKDITIICYGAMLHPTL
                     EAAKELKEKDGVEAEVIDLLTISPLDDELFSQSVRKTGRAMVVHEAPRSFGPGAEVAT
                     RLMERSFFHLEAPVKRVTGYDVIIPLFSRENTYLPNVQRILSAARETLRV"
     gene            complement(5211..6428)
                     /gene="pdhA"
                     /locus_tag="VHS06_06380"
     CDS             complement(5211..6428)
                     /gene="pdhA"
                     /locus_tag="VHS06_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX2987781.1"
                     /translation="MPRKAIPLPYQVDYLSVLDENGHLDKELEPDMPDELLLKLHRAM
                     LLTRRFDERMLDLQRQGRIGTFGPIKGHEAAHLGAVATIRDSDWMVPSFRENGAELWR
                     GRTMESFLLYYAGYDEGARIEDGRNDLPIAVPIASQTLHAVGLGFGINYRKTDQVVLV
                     FFGDGATSEGDFHEAMNFAAVFQTPVVFVCQNNQWAISVPRDRQTRSETLAQKVVAYG
                     MPGIQVDGNDILSVYAATKEAVDRARSGGGPTMIECVTYRVVMHTTSDDPKRYRSETE
                     AQDWMKKDPLVRFQRYLIGRKLLTDKKIEALEAEVKAEIQASVERFEQQAAALGDPLD
                     MFNHAYAEMPSTLLEQREELRRELGANGHTTAADMDDTAAIIRQIFTEQPLKATATLR
                     PEVAANGEETGRG"
     gene            complement(6443..>7212)
                     /locus_tag="VHS06_06385"
     CDS             complement(6443..>7212)
                     /locus_tag="VHS06_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987782.1"
                     /translation="TSELTAEQVAKKGLEEGDIDWEAATEPRLQPASAKDQAGFSEEH
                     RRLKQLVVEMNGCERARRDIAGWRRDIQIRSRGGNLGLRLSDGFLEITDLFCLPTSEL
                     TAEQVAKKGLEEGDIDWEAATEPRLQPASAKDQAGFSEEHRRLKQLVVEMNGCERARR
                     DIAGWQRDIQVKSRGGNLGLRLSDGFLEITEGEIGSPDFVMVCDDLSVLTRSLAYQGS
                     LTQAIINRELWISKNGEFTTVFKLERMARSLARSRKG"
BASE COUNT         1371 a         2245 c         2262 g         1334 t
ORIGIN      
        1 gtcatcccga gcgtggcgaa cggaaacgcg ggcgcttcgc tcaacaggga caggggacac
       61 aaccatggaa gaggtcgtga tcgtcggggc ggctaggacc cccataggga aactgctggg
      121 ggcgctctcg acgctaccca ccccccaact gggggcgacg gctattcgcg ctgcgttaga
      181 gcggggtggg attgcaccgg agaatgtgga tgaggtcgtg atgggcaacg tcctggcggc
      241 agggataggg atggccccgg ccaggcaggc ggcaatcgcc gccgggatcc ccgcgtcggc
      301 ctcggcgcta accgtcaaca aggtgtgcgc ctcgggatta gctgcggtcg tcgtggcgat
      361 ccaggagata agggatggag cggcgaacgt ggttgtggca ggcgggatgg agaacatgag
      421 cctcgccccc ccacctgctg ccgggcacac gcaccggggt caagctaggt ggaatggggc
      481 tcatcgactc ggcaatccac gatggcctct ggtgcgcgtg ggaagaccac cacatgggat
      541 gcgccgccga ggagatcgcg cggaagtacg ggatcacccg tgctgagcaa gacgctcacg
      601 ccttcgagag ccacagaagg gccctggcag ccatagacgc gggccgcttc cgtcccgaga
      661 tcgcgccggt ggaactgcaa ctgaaaggta aggcggtgct gatagacacc gacgaatgcc
      721 cccgccgaga cacgtccatg gaggcgctgg gacgcttggc gtccgcattc gaatccggcg
      781 ggtcggtgac cgcgggcaac tctccaggct tgtgtgatgg cgcagcagcg ttcgtgatga
      841 cctcctccgg gctcgccagg gagagagggt ggcagaccag ggcgcgcatc accggttacg
      901 tgagcgcgaa cctggacccg cggtggctgt tcgacgcgcc tgccgaggcc gtgcagcgac
      961 tgctgaagaa aacgggccaa tccctggccg acttcgatct gatcgaaata aacgaagcct
     1021 tctccgccgt cgttctggcc gcccagaggg tgctgaagtg ggaccccgac agggtgaacg
     1081 tgaagggcgg ggccgtagcg ctgggacatc ccataggatg cagtggcgca cggatccttg
     1141 tgaccctgat ccacgccctg gaggagatgg atctgcacaa agggctggcc gtgctctgcc
     1201 acggaggagg cggtgcggtg gcagtgagcg tggagcggta aacatggatt tcgaactcac
     1261 accagaacag aaatcgcttc aagaggaggc ccaggcattc gcggcagcag agttcgaccc
     1321 tgcttatgcg atgcgttgtg agcaggaccg ccgctatcca tctgatctgc gccagaaggc
     1381 agggactcgc ggtctgctgg ctgtgtacat accccgggag tatggcgggc gagggttggg
     1441 cctcctggaa tcggtgctgg taatcgaggc gatgtgccgc aaggatccgg gtctgggcat
     1501 ggccctcagc cttgcctcgc tgggaacccc cgtcaccctg aggtacggga gtgaagaaca
     1561 aaagaggacc attctcccca gggtagctgc cggggagatc atgacgggca tcgcactcac
     1621 cgaaccgggc cacgggagcg acctgacgga cgtagccacg accgccacca ggacgagcag
     1681 cggctacgtc gtcgagggcc gcaagacgct catttccaac gggcagaatg cccgctacgt
     1741 cacgttgcta tgtaagacgg accccgaggc gaggccgccc catcgtgggc tcagcctgat
     1801 ccttctggat accaatcggc cgggctacaa ggcatccgac ctggggttga agatgggcat
     1861 gcggatgatg tccacggctg agatcagtct ccagggcgtc aaggtcccgg caagtaacct
     1921 gatcgggatg gagaatcgag gcttctacca cacactcgag ttcctgaacg agtccaggat
     1981 cgaggtggct gctcaggcgt tgggggatgc ggaggccgcc ttcgaccgcg cgctggcccg
     2041 tgtcaaggag aggcgccagt tcgggaaaag gctcgcggac ctacaagtga cgcaacacaa
     2101 gatcgcagac atggcgacca agatcgagac ggctcgactc cttacctaca tggccgcatg
     2161 gcaatctgac caaggtggaa gcgatccggt gcaggcatct atggcaaagc ttcatgccgc
     2221 tcgcgtcgcc gtcgaggtag ccgaagaggc tatacagctg ttcggcggat acggctactt
     2281 cctggagaac gaggtagaac ggatctaccg agacgcacgc gtgacggaga tctacgaggg
     2341 cacccgagag atccagaaga acaccatcgc ccgatatctg ctgggatctt ggcgagagga
     2401 agcgtgagga atatggcggg gcgggttggg atagtcgggg cggctgtgac cccattccac
     2461 agcaagtggt acgagaagac ctactacgaa ctggcccaga tggccacagc ggaggcactc
     2521 aaggacgccg gactgtcgcc agaagaggtg gacggcgtct tttacgggat atacaacgac
     2581 atctttcagc ggacctgcat tccggagcac cccctccagg ggttgatcgg aatgcagaac
     2641 aagtttggga tccgcatctc caacggaggt gccaccgggg cctacaccct atccgccgcg
     2701 cacgcctacg tagccgcggg gcgattcaag gccgcgctgg tgctgggggt ggagaaatcc
     2761 accgactgtt tcgactatca atccatgtcg gcaacaccgg aggtgatcaa gaccatcggc
     2821 tggtccggcg acagcttctt cgagcagaag ctggggtgga ccgcctcgga ctcgtacgcc
     2881 gaggtggtgc tggcatacat ggacgagcac ccggatgacc tgaagccgga gatcaccgcg
     2941 caggtctcgg cgttgctgag ccggcatgtg aagggcaact ccttcgccca acgccagcac
     3001 gaccaggtca cgcctgaaga ggtgatgaac tcccgcatag tcgtgtaccc cttcaaggag
     3061 ttggagatct gcgtctactc cgaaggggcc gcggcactca tcgtcgccga ggaggatacg
     3121 gcgaaggcca tctcccgcaa caccggcaat ccggttatct ggatctcggg cgtcggcgag
     3181 tcgaacgaac actccttcgc tgggaagaat cagaaggtga tgcaccggat catgtccgac
     3241 catctggcag cctaccgagc ctacgagatg gcaggggtga aggatccgct taaggagata
     3301 gatgtggtgg aggcacacga tgccttcgtc ccgcagctag agataaccat ggcagagatg
     3361 ggcttcgcgc ccctggggag ggcggacagt ctgatcgagg aggggatcgt cactcccggt
     3421 ggtccgatca tcctgaaccc cagcggtggc ctgatctact gtggccacgc cgtgggcgcc
     3481 agcaacatca tgtcggcatg gtcggccagg aaagagttga tcaatcgggg attgcgccgg
     3541 gccctggttc acggcaccgg cagcacggtt gcccagtacg gagtcgcctt cgtgctggag
     3601 cacgactaat cggagccaca caatggccaa gaaactgata cctaccgagg caatccccga
     3661 gcagggcggg gagatcgtca gcatcgacgg caagggctac ctgatgctaa acgatgcaat
     3721 gttcaccttc taccgccgat ccatggggga gctaacccca ttcttcctcg ccttacgaga
     3781 cgaaaagaag atcctgggcg cccgctgcac gaactgcggc attgtgcggg tgcccccctt
     3841 cgccacccgc tgcccggact gcaacttcgc ccccaccgaa ttggtcgagg ttggagacgt
     3901 cggcaggatg ctgaacaccc cgcccatcac ctacttcgcc aacgccctct ttcagcagca
     3961 ggttcccttc ggcaggggca gggtggttct ggaaggggca gacaccgcca taagcgtcaa
     4021 catatacacc agcaaaggga tcctggtgcc cggtctgatc accaaggaca ccgaggtgaa
     4081 ggtggttttc cgtaaggatc gcaccggcga gataaccgat ctcttctgcc cttcggtgac
     4141 gtgatcgccc tcggacacca ggacggcgat cacctcgccc tcgtggatac cttctcccag
     4201 gtcaggcagt ttgaacggcc gagccatcgc actcctccta cacccgcagc gtctctcggg
     4261 ccgcgctcag tatcctctgc acgttgggca ggtaggtgtt ctctcgactg aagagcggga
     4321 tgatcacgtc gtagccggtg actcgtttca ccggcgcctc cagatggaag aaggatcttt
     4381 ccatgaggcg ggtagccacc tcggcacccg gcccaaagct tcgcggtgcc tcgtgtacga
     4441 ccatggcgcg gccggtcttt cgcacggatt ggctgaacaa ttcgtcgtcc aggggcgaga
     4501 tggtcaagag gtcgatcact tcggcttcga ccccatcttt ctccttcaac tccttagccg
     4561 cctcgagggt cggatggagc atggcgccgt agcagattat ggtgatgtct ttcccttccc
     4621 gaactacctg ggacttgccg aggggcaagg tctcctcggc ctccggcacc tcttctctga
     4681 atgccctgta cacggccttc ggctcgtaga agatcaccgg atctgggtcg cggatggcgc
     4741 tcaccagcag agcgcgggca ttgcggggac cggatgggat caccatcttc agccccggga
     4801 tgtgggacca gaacgcctca cggctttccg agtggtgctc caacgccctc acgccggccc
     4861 catagggagc acgcagcacc atgggcacgg tgtgtcttcc ctgggagcga tggcgcagcc
     4921 gcgaggcatg actctcgatc tgggaaaagt tcaggtagtc gaaccccgcg aactgtatct
     4981 cacacaccgg ccgaagcccg taaaccgcca ttccgatgga ggtgccgacg atgccagatt
     5041 ctgccagcgg cgtgtccacg acgcggtcct cgccgaactg ctccaccagc ccctctgtga
     5101 ctcgaaagac gccgccgtcc ttccctatgt cctcgccgag gatgatgacc cggtcgtcct
     5161 tctccatctc ctgtcgcagc gccaggttta atgcctggac catagtcatc ttagccacgg
     5221 ccggtctcct ctccgttcgc cgcgacctcc ggacgcaggg ttgcggtcgc cttgaggggt
     5281 tgttctgtga agatctgcct gatgattgcg gctgtatcgt ccatgtcggc ggccgtggtg
     5341 tgcccgttcg ccccgagctc tctccgcagc tcctcgcgct gctccagcag cgtcgagggc
     5401 atctcggcat aggcgtggtt gaacatgtcg agaggatcgc ccagggccgc ggcctgctgc
     5461 tcgaagcgct ccacggatgc ctgtatctcg gccttgacct cggcctccag cgcctcgatc
     5521 ttcttgtctg ttagcagctt ccggccgatc agataccgtt ggaatcggac gagcggatcc
     5581 ttcttcatcc aatcctgcgc ttccgtctcg gagcgatatc gcttgggatc atcggaggtg
     5641 gtgtgcatga ctacccggta ggtcacgcac tcgatcatag tgggaccgcc accggagcgt
     5701 gcccgatcca ccgcctcctt cgttgccgcg tacaccgaca ggatgtcgtt gccgtccacc
     5761 tggatccccg gcatcccata ggcgaccacc ttctgcgcca gggtctcgga gcgggtctgt
     5821 ctgtcgcgcg ggacggagat ggcccactgg ttgttctggc agacaaacac caccggggtc
     5881 tgaaagacgg cggcgaagtt catcgcctcg tggaagtcgc cctcggaggt ggcgccgtcc
     5941 ccgaagaaga cgaggaccac ctgatcggtc ttcctatagt tgatcccgaa tccgagtccg
     6001 actgcgtgga gtgtctggga cgcgataggt actgcgatgg ggagatcgtt gcggccatcc
     6061 tcgatcctgg cgccctcgtc gtagccggcg tagtagagca ggaagctctc catggtgcgg
     6121 ccgcgccaca gttccgctcc attctccctg aacgagggga ccatccagtc ggagtcgcgg
     6181 atggtagcca cggcgccgag atgagcagcc tcgtgcccct tgatcggccc gaaggtgccg
     6241 attcttccct gccgctgcag atcgagcatc ctctcgtcga atcgcctggt cagtagcatc
     6301 gcccgatgga gtttcagcag aagctcgtcg ggcatatccg gttcaagctc cttgtccagg
     6361 tggccgttct cgtctaagac ggataggtag tccacctggt aagggagcgg tattgctttc
     6421 ctgggcatct ctcgaaccct tcctaacctt ttctagagcg ggcgagagag cgggccattc
     6481 gctccagctt gaatactgtc gtgaactccc cgttcttgct gatccacagt tcgcgattga
     6541 tgatggcctg ggtaagcgat ccctggtagg caaggctgcg ggtcaacact gacaggtcgt
     6601 cgcacaccat cacgaagtcg ggcgaaccga tctctccctc ggttatctcc aggaatccgt
     6661 cgctcaatct cagtcccaga ttgccgcccc gcgacttgac ctggatgtcg cgctgccatc
     6721 cggcgatgtc tcgccgagcc cgctcgcatc cgttcatctc cacgaccagc tgtttcagcc
     6781 ttcgatgctc ctcggaaaag cctgcctggt cctttgccga cgccggctgc agccgcggct
     6841 cggttgcggc ctcccagtcg atgtcgccct cctccagccc cttcttagcc acctgctccg
     6901 ccgtcagttc cgaagtgggt aggcagaaga gatcggttat ctccaggaat ccgtcgctca
     6961 atctcaggcc cagattgccg ccccgcgacc tgatctggat gtcgcgcctc cacccggcga
     7021 tgtcccgccg agcccgctcg catccgttca tctccacgac cagctgtttc agccttcgat
     7081 gctcctcgga aaagcctgcc tggtcctttg ccgatgccgg ctgcagccgc ggctcggttg
     7141 cggcctccca gtcgatgtcg ccctcctcca gccccttctt agccacctgc tccgccgtca
     7201 gttccgaagt gg
//
LOCUS       DATALS010000288         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_163379, whole genome shotgun sequence.
ACCESSION   DATALS010000288 DATALS010000000
VERSION     DATALS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163379"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..>2151
                     /locus_tag="VHS06_06390"
     CDS             116..>2151
                     /locus_tag="VHS06_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987783.1"
                     /translation="MVRESMGRHGDSETRRNGDAETRGRGDAGSAHPLFERLLPEGEA
                     VTKSPLPLGEGRGEGSTQHSAPSTHDSAPSTQHPTAPRTQNPEPRTHPPLRRRLPGIL
                     LPALWTGMALALLAACDYILFRPAIVDGQIYSRSDTVTYYFPIANRLIDVLKEGRLIL
                     WTRYLFGGFPLFADGEAGMLYPPHLLTYLLLPEQDAIIWSRVLRHALAALFCFGYLRT
                     LRLNRFGAVIGAIIFAYGSFLITQMHHSNVANTAIWLPLTLAMIEMSLRNVRRWRWIY
                     AGAAGMSVGVQALGLHIQPLLMSGLFLAFYIPFRVMLCPIAWPEGWVNRLPSCLGRGS
                     FVGSVVGRAGRGLWTALSPFRRLLAPVAGRGTRWLRHRWRRDFFGLRLILLGPDLVMG
                     AGDGQAVMTDRPSGEEGDGKGQELSPAVVVPWWSRHPALTLSSRGGVWAGRFISRFVA
                     AAFHRGVLALLLGIGIPAIAFGLAAVQILPLVELGTFSFRGPGVNYQFATSYSFPIQN
                     LIDLVFPYFFRYTNRYYWSLWSEWETTVYVSIAGLVLASIALIFVRNRLVLFFAAFAT
                     FSMLLCFGGYSPYPLIDWVRQLPGFSSLRVPGRYSMLFTFCIAVLAAYGADWLCKNLR
                     PDVRTAHWHWRARISRSLSVNGFAVYLVGLLASMAFVVWWLLSFRIWIEKEPWAA"
BASE COUNT          322 a          669 c          676 g          484 t
ORIGIN      
        1 cgagatcgaa ccggctggcc gtgggtaact cctacctgta tgagccctcc accggcatcc
       61 tgcgggttcg ctacaaccat cgacgtcctc acaccctgga agtgaggtac ttgccgtggt
      121 gagggagagt atggggcgac acggcgactc ggagacacgg cgaaacggag acgcggagac
      181 gcggggacgc ggagacgcgg ggagcgctca cccgctcttc gagcggcttc tcccagaggg
      241 agaggcggtt accaagtccc ctctccctct gggagagggt cggggtgagg gcagcaccca
      301 gcactcagca cccagcaccc acgattcagc acccagcact cagcacccga ctgcacccag
      361 aacccagaac ccagaaccca gaactcatcc cccgctccgg cgccgccttc cgggtatcct
      421 gctccccgcc ctgtggaccg gtatggcgct ggcgctccta gccgcctgcg actacatctt
      481 gttcagaccg gccatagtcg atggccagat ctactcacgc tcggacaccg tcacctacta
      541 cttccccata gccaaccggc tgatcgacgt gctgaaggag gggaggctga tcctgtggac
      601 ccgttacctg tttggcgggt ttcccctttt cgcggatggc gaggccggga tgctctaccc
      661 accgcatttg ctcacctacc tgttgttgcc tgaacaggac gcgatcatct ggtcgagggt
      721 gctgcgtcac gcgctggccg cgctcttctg ctttggttac ctgcgcacgc tccgcctcaa
      781 ccgtttcggc gcggttatcg gtgccatcat cttcgcctat ggcagcttcc tgatcactca
      841 gatgcaccac agcaatgtgg ccaataccgc catctggctg ccgctcacgc tggcgatgat
      901 agagatgtcg ctgcggaacg ttaggcgctg gcgctggatc tatgccggcg cggccggcat
      961 gtccgtcggc gttcaggcat tggggctgca catccagccg ctgctgatga gcggcctgtt
     1021 cctcgctttc tacatcccgt tccgagtgat gctgtgccct attgcgtggc ctgagggctg
     1081 ggtaaacagg ctgccgtctt gccttgggcg tggcagcttc gtgggaagcg tggtcggtcg
     1141 tgctggccgg ggcctgtgga cagcgctgtc cccgtttcgg aggctcctgg ctccagtggc
     1201 cggtcgtgga acgaggtggc tgcggcatcg gtggaggcgg gatttctttg ggcttcggct
     1261 gatactgctt ggccccgacc tcgtgatggg tgccggggat gggcaggcgg tcatgaccga
     1321 taggccgtca ggcgaggagg gtgacgggaa aggtcaggag ttgtctcctg ctgttgtggt
     1381 gccatggtgg tcacgtcatc ctgccttaac gctctccagt cgagggggag tctgggctgg
     1441 acggttcatc tctcgtttcg tcgcggctgc cttccatcgt ggggttctgg cactgttgtt
     1501 ggggatcggg atcccggcga tcgctttcgg gcttgcagct gtgcagattc tgccgctcgt
     1561 cgagttgggc accttctcct tccgtggccc gggggtcaac taccagtttg ccacctccta
     1621 ctcgttcccg attcagaact tgatcgatct ggtattcccg tacttcttcc gctacaccaa
     1681 tcgctactac tggtcgctct ggtcggaatg ggagactacg gtctacgtga gcatcgcggg
     1741 gttggtgctg gcgagcatcg ccctcatctt tgtccgcaat cggctggtgc tcttcttcgc
     1801 cgcctttgcg accttcagca tgctgctctg cttcggtggg tactcgccat accctttgat
     1861 cgactgggtt aggcaactgc ccggcttctc gtcgctacgg gttccgggcc gctacagcat
     1921 gctgttcacc ttctgcatcg cggtgctggc cgcctacggg gccgattggc tctgcaaaaa
     1981 cttgcgaccg gatgtacgga cagcgcactg gcactggagg gcgagaatct ccagatcgct
     2041 gagcgtcaac ggctttgccg tctatctggt tgggttgctc gcctccatgg ccttcgtcgt
     2101 gtggtggctg ctcagtttcc gcatctggat cgagaaggag ccctgggcag c
//
LOCUS       DATALS010000289         6215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_253505, whole genome shotgun sequence.
ACCESSION   DATALS010000289 DATALS010000000
VERSION     DATALS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253505"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2076
                     /locus_tag="VHS06_06395"
     CDS             <1..2076
                     /locus_tag="VHS06_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2987784.1"
                     /translation="LLDKQDVEYCQYLITIGGTLGPNIGSAHSIRALSGARERGMKIV
                     VVDPRCGHEASMADEWIPIRPGTDLAFSLAMLNVMMNEIGVYDEQFIKRRTNAVYLIG
                     PDGYYLKDGASGKPLVWDSVEGRAKCFDDPSIVDYALEGSFLVEGLQADVAFGLIKKD
                     VRQYTPEWAEEITTVPAATLQRISREFVEAAHIGETIELDGFQFPFRPAVMKAERGTL
                     GKKGGAYQHLASKMICMLVGALDVPGGYLGSQTGPVLSPGPDGVVSPKSEAVGVPFSY
                     PPDVDLKQYYPNRHTMPHMAWKAILDPAKYGVPYDVEALMIYGGNPITNNANPDEAIA
                     ALSKIPFVVTIAYLYDEAAELSDILLPESALLERHGIQKLGDHFVQAVDEISLRVHGV
                     LVRQPVVKPLHNTRQADEIYMELAERIGFLYGEGGLNDRINRCWSIEEPHKLELDRRY
                     TTPEIMDRMLKSVYGVEDGLEHFQNAAVEYHMTSKAESYNYFYYPMGKTRHPFYFEHL
                     LRTGDTLRRNLDEHGATIPGQDMADVWSYYRPIPRWTGRPDDSAQPQYDLVAMNWATP
                     QFRMNCGDQTGNPLLNEVVDRTDPYQFAVLINRETARQKGLNDGDRVVVEAYWGGKAE
                     GTLRVTDLIHPDAVGIPAIHGFRSIHRNPVTRRGPHFNSLLSSAEGTFDPVHGGIDRS
                     PRVRVYRAT"
     gene            2215..2838
                     /locus_tag="VHS06_06400"
     CDS             2215..2838
                     /locus_tag="VHS06_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2987785.1"
                     /translation="MRYAMVIDLRKCVGCNACTVACKQENATPAGVYWSKVLQYEVGS
                     YPNARLRFLPMLCMHCANAACLEACPTGATHRGPGGEVLVDDDLCICCRYCMMACPYE
                     ARSYNETSPREYHPDRGLTPAEEQGYISHPRGAIEKCTFCSGRVEQGKQPACAATCPA
                     GARIFGDLDDPNSEVAKLVASGLAKPRLAELGTEPSVFYIDLETHSS"
     gene            complement(2956..4026)
                     /locus_tag="VHS06_06405"
     CDS             complement(2956..4026)
                     /locus_tag="VHS06_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2987786.1"
                     /translation="MSPAAGIPSTMKAAVIRAWNEVHVEEVPIPAFGPGEVLIRVKAC
                     GICPTDLRIVAGAYAPHWPKELPFIYGHEWVGQVAAVGEGVTNVKVGDRVAGENHIGC
                     RECRMCRQGRYNLCDRAEDGTQVVYGHDAPGAFSEYISRPAMALHPMPDAVSFEDGVI
                     INQGAMVLHGANRAEIGPGDSVAISGPGLCGLLALQIARAKGATSIIMIGRGHRLEVA
                     KELGADHVVDITREDPAKAVQALTGGLGADVGMECSGNPDALEPLTYAVRRGARVAVI
                     GLNGKKQVPIITDRVALDEVDLIGVRGSPGEYPRFISLMAAGMVSARGLVTHTFPLAE
                     INEAMQAFRERRGNAIRVAIQI"
     gene            complement(4026..4784)
                     /locus_tag="VHS06_06410"
     CDS             complement(4026..4784)
                     /locus_tag="VHS06_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX2987787.1"
                     /translation="MGWGNSLFEITAAEAEQKFDECNIAVIPMGSVEQHGPHLPMGTD
                     AMAGIAFGKRVAEKLNAVFVPFSVIGVTPYHMDWKGTITLQNSTFIALFKDVVESLYH
                     HGTRNILVSNGHEGNIAPVRVASDELQQKHRDLRIIIADTWVVVNNLFPELERTHAAE
                     MEAQEAMCYDPALCHLERGTNPSDLEFGRVGHERFRKKDVYTMFRNFKEVAATGWYGS
                     LDRAKVERVPEVLEKVSDVIAEQASAMFKDLEGF"
     gene            complement(4850..5401)
                     /locus_tag="VHS06_06415"
     CDS             complement(4850..5401)
                     /locus_tag="VHS06_06415"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX2987788.1"
                     /translation="MRLDRSAHWIESWARPFSAGMSTVGAVALLIMMFWVVADVLLRF
                     LFDRPLLGSYEIVEYMMVAFVFMSFAYAQFCKAHISVPVVVERLGPRARAVLDIITGI
                     ITLLVTAVMVWGALKQTQDMWAAHMTSAVLFIPKWPFQLITGIGLLAFCVAKLVDVLM
                     DLYQISAGKVVVPESSAEELRSI"
     gene            complement(5505..>6215)
                     /gene="dctP"
                     /locus_tag="VHS06_06420"
     CDS             complement(5505..>6215)
                     /gene="dctP"
                     /locus_tag="VHS06_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX2987789.1"
                     /translation="YTLPVTGITTSKWGALSMWDLYQGNADIQTEGESVKLLGFTSSG
                     TQWISTAKKPVRTIDDVKGLKLRIAGWGGTEYLKSLNASPIAMAPPDMYESLSKGILD
                     GIVFDWQGINSSRLYEVVSYASTMPVVMQPQAIMMNQAKWKALPPDIQKVFTDMSGKW
                     LAETGGNEAFDAADQEGIDNFKKLNKEIITYSPEEKAKWQEAAKPIWAAWVKNVKDKG
                     LDGQKALDGLQAAIAKNK"
BASE COUNT         1166 a         2101 c         1853 g         1095 t
ORIGIN      
        1 ctcctggaca agcaggacgt ggagtactgc cagtacctca tcaccatcgg gggaaccctc
       61 gggccaaaca tcggcagcgc ccactccatc cgcgccctca gcggcgcgcg ggagcggggc
      121 atgaagatcg tggttgtgga ccctcgctgc ggccacgagg catccatggc ggacgagtgg
      181 atcccaattc gccccggtac ggacctggcc ttcagcctgg cgatgctcaa cgtgatgatg
      241 aacgagatcg gcgtgtacga cgagcagttc atcaagcgcc ggaccaacgc cgtctacctg
      301 atcggcccgg acggctacta cctcaaggac ggcgccagcg ggaagccgtt ggtctgggac
      361 tcggttgagg gcagggccaa gtgcttcgac gatccctcca tcgtggacta cgccctggag
      421 ggaagcttcc tggtggaggg gctacaggcc gacgtggcct tcggcctgat caagaaggac
      481 gtgagacagt acacccccga gtgggcggag gagatcacca ccgtcccggc cgcaacgctt
      541 cagcgcatct ccagagagtt cgtcgaggcc gcgcacatcg gggagaccat cgagctggat
      601 ggattccagt tccccttccg accggccgtg atgaaggccg agcgcgggac gctgggcaag
      661 aagggcggag cctaccagca cctggcgagc aagatgatct gcatgctggt gggggccctg
      721 gacgtgcccg gcggctacct gggatcgcag accggcccgg tcctgagccc ggggccggat
      781 ggagtggtat cccccaagag cgaggccgtg ggggtcccct tcagctaccc gccggacgtg
      841 gacctcaagc agtactaccc caaccgccac accatgcccc acatggcgtg gaaggccatc
      901 ctggaccctg ccaagtacgg cgtcccgtac gacgtggaag cgttgatgat ctatggcggc
      961 aaccccataa ccaacaacgc caatccggat gaggcgatcg ccgccctctc caagataccg
     1021 ttcgtggtga ccattgctta cctctacgac gaggccgcgg agctttccga catcctgctg
     1081 ccggagagcg cactgctgga aaggcacggg atacagaagc tgggcgacca cttcgtccag
     1141 gccgtcgacg agatcagcct gcgggtccac ggtgtgctgg tccggcaacc ggtggtgaag
     1201 ccgctgcaca acacccgaca ggcggacgag atctacatgg agctggcgga gcgaatcggc
     1261 ttcctgtacg gagagggcgg gctcaacgac cgcatcaacc gctgctggag tatcgaggag
     1321 ccccacaagc tggagctgga caggcgctac accactccgg agatcatgga ccggatgctg
     1381 aaatccgtct acggcgtgga ggatgggctg gagcatttcc agaacgccgc cgtggaatac
     1441 catatgacca gcaaggcaga gtcgtacaac tacttctact accccatggg caaaacccgc
     1501 caccccttct acttcgagca cctgctccgc acgggggata ccctccgccg aaacctggac
     1561 gagcacggcg caaccatccc cggccaggac atggcggacg tctggagcta ctaccggccg
     1621 atccctcgct ggaccggccg gccggatgat tccgcccagc cccagtacga tctcgtggcg
     1681 atgaactggg caacccccca gttccgcatg aactgcggcg accagacggg caatccgctg
     1741 ctgaacgagg tggtggaccg gaccgaccct taccagttcg cggtgctgat caacagggag
     1801 acggcccggc agaaagggct gaacgacggc gatcgagtgg tggtggaggc gtactggggg
     1861 ggcaaggcgg agggcacgct gagagtgacc gacctgatcc acccggatgc ggtagggata
     1921 ccggctatcc acggcttccg ctccatccac cgcaacccgg tgacgcggcg gggtccgcac
     1981 ttcaactcgc tgctctccag cgcggagggc accttcgacc ccgtgcacgg cgggatagac
     2041 cggagccccc gcgtgcgggt gtacagagcc acgtaaacca aatggagtgg aggcttcagc
     2101 cggaaggcac tcgtatcaac aggtacgttc cggctaaagc ctccactcca tctattcatt
     2161 gaagccgcgc gggcggcaca ccaaccatgg gaaccgatcc gaagaggtag acctatgaga
     2221 tacgccatgg tcatagatct gcgcaagtgc gttggatgca acgcctgcac cgtcgcgtgc
     2281 aagcaggaga acgccacccc cgcgggggtc tactggagca aggtgctcca gtacgaggta
     2341 ggctcctatc ccaacgcgcg cctccgattc ctccccatgc tctgcatgca ctgcgcgaac
     2401 gccgcctgcc tcgaagcctg ccccaccggc gccacacacc gagggccggg gggtgaggtg
     2461 ctggtggacg acgacctctg catctgctgc cgctactgca tgatggcctg cccgtacgag
     2521 gcgcgcagct acaacgaaac ctcgccacgc gagtaccacc ccgatcgggg cctcaccccc
     2581 gccgaggagc agggctacat atcccaccca cgcggcgcta tagagaagtg caccttctgc
     2641 tccggcaggg ttgagcaggg gaaacagccg gcatgcgcgg ccacctgccc cgccggcgcc
     2701 agaatcttcg gggacctgga cgatcccaac tcggaggtgg ctaagctggt ggccagcgga
     2761 ctggccaaac cgcggttggc ggagcttggc accgagccct cggtgttcta catagacctc
     2821 gagacgcact cctcgtagca cgcgccgcac tccatatccc tcgatgcgca caggaagaac
     2881 cccgctcctg caccgaggtg ctacggagcg gggttctcca ttgcctccaa cttggactat
     2941 gccctcatcc tgggcttaga tctggatggc tacccggatc gcgtttccgc gtcgctcgcg
     3001 gaatgcctgc atcgcctcgt tgatctccgc cagcgggaag gtgtgggtca ccaaccccct
     3061 ggcgctgacc atacccgccg ccatcagcga gatgaacctg ggatactcgc cggggctccc
     3121 acgcacccca atcagatcca cctcatccag agccacccga tccgtgatga tgggcacctg
     3181 cttctttccg ttcaggccga tcaccgccac acgggcgccc ctccgaacgg cataggtgag
     3241 gggctcaagc gcgtccgggt tcccggagca ctccatcccc acgtccgcac cgagaccacc
     3301 ggtcaacgcc tgcaccgcct tcgccggatc ctcgcgcgtt atgtccacta catggtccgc
     3361 gcccagctcc ttcgcgacct cgaggcgatg ccctcgcccg atcatgatga tgctggtggc
     3421 gcccttagct cgggcaattt gcagcgccag caggccgcac agtccagggc cggagatagc
     3481 aacggagtcg ccggggccga tctcggcccg attggcgccg tggagcacca tcgccccctg
     3541 gttgatgatc acgccgtcct caaagctcac cgcatccggc atggggtgaa gcgccatggc
     3601 cggccgcgaa atgtactcgc tgaacgcacc gggcgcatcg tggccgtaga caacctgggt
     3661 cccatcctcg gcccgatcac acaggttgta ccgcccctgg cggcacatcc ggcactcacg
     3721 gcagccgata tggttctcgc cggctacgcg gtcgcccacc ttgacgttgg tgaccccctc
     3781 gccaaccgcc gccacctggc cgacccactc gtggccgtat atgaagggca gctccttcgg
     3841 ccagtgagga gcgtaggctc cagccacaat ccgcagatcg gtagggcaga tgccgcaggc
     3901 cttcaccctg atcagcacct ccccgggccc gaacgcggga attggaacct cctccacgtg
     3961 cacttcattc caggcgcgga tcaccgcggc cttcattgtg gaagggatcc cagctgccgg
     4021 agacattaaa agccctccag gtccttgaac atcgcgctgg cctgctccgc gatgacgtcg
     4081 gagaccttct ccagaacctc aggaaccctc tctaccttgg cccgatccag ggagccatac
     4141 catccggtgg cggcgacttc cttgaagttg cggaacatgg tgtacacgtc cttcttccgg
     4201 aaccgctcgt gcccgacccg accgaactcc agatcagacg ggttggtgcc cctctccaga
     4261 tggcagaggg cgggatcgta gcacatggct tcctgggcct ccatctccgc ggcgtgagtc
     4321 ctctccagct cggggaacag gttgttcacc acaacccagg tgtcggcgat gatgatgcgc
     4381 agatcccgat gcttctgctg caactcgtcc gaggccactc ggacgggggc aatgttcccc
     4441 tcatgcccat tgctcaccag gatgtttcgg gtgccgtgat ggtaaaggct ctccaccacg
     4501 tccttgaaca gggcgatgaa ggtcgagttc tgcagggtta tggtgccctt ccagtccatg
     4561 tggtaggggg ttacgccgat gaccgagaag gggacaaaca ccgcgttcag cttctccgcc
     4621 actcgcttgc cgaaggcgat gcccgccatc gcatcggtgc ccatcggcag gtgaggtccg
     4681 tgctgctcca cgctgcccat tggaatgacg gcgatgttgc actcgtcgaa cttctgctcc
     4741 gcctcggccg cggtgatctc gaacagggag tttccccagc ccatgatatt tcctcctgca
     4801 ccttgagatt tattgtctcg cctccaggcg gtaagacccc gtgtcgcccc tagatactcc
     4861 tcagctcctc tgcgctactc tccggtacga cgacttttcc ggcgctgatc tggtacaggt
     4921 ccataagcac atccaccagc ttggcgacgc agaaggccag cagcccaatg ccggtgatca
     4981 gttggaacgg ccacttggga ataaacagga ccgcggaggt catgtgggcc gcccacatgt
     5041 cctgggtctg cttgagggcc ccccagacca tcaccgctgt caccagcagc gtgatgatcc
     5101 cggtgattat gtccagcacg gccctggccc tcggccccag cctctccacc accacgggta
     5161 cgctgatgtg cgccttgcag aattgggcgt aggcaaagga catgaagacg aaggcgacca
     5221 tcatgtactc tacgatctcg taagagccaa gcagcggccg gtcgaacagg aaacgcagca
     5281 gcacgtcggc aacgacccag aacatcatta tcagcagggc gaccgcgcca accgtgctca
     5341 ttccggcact gaaaggccgg gcccaactct cgatccagtg cgccgaacga tccagcctca
     5401 tctccctggc cctcctcgat catccgaaag gtgcaaaccc accttgtgcc gcttccgtgg
     5461 ggccggctcg cgcccggccc cgagtgttca accaggatcg agtgttactt gttcttggcg
     5521 atggctgcct gcaacccgtc cagcgccttc tggccatcca gccccttatc cttcacgttc
     5581 ttcacccagg cggcccagat gggcttggca gcttcttgcc acttggcctt ctcctcggga
     5641 gagtaggtga tgatctcctt gttgagcttc ttgaagttgt ctatgccctc ctgatcggct
     5701 gcatcgaatg cctcgttgcc gccggtctcg gccagccact tgccgctcat gtcggtgaac
     5761 accttctgga tgtccggcgg cagcgccttc cactttgcct ggttcatcat gatggcctgg
     5821 ggctgcatca ccaccggcat ggtggaggcg tagctcacga cctcatacag ccggctgctg
     5881 ttgatcccct gccagtcgaa cactatgccg tccaggatgc ccttgctgag cgactcatac
     5941 atgtccggcg gcgccatggc gatggggctg gcgttcaggc tcttcaggta ctccgtgcct
     6001 ccccagcccg ctatgcgcag cttcaaaccc ttcacatcgt cgatggtgcg gaccggcttc
     6061 ttggcggtgc tgatccactg ggtgccgctg ctggtgaagc cgagcagctt tacgctctcc
     6121 ccctccgtct ggatgtccgc gttcccctgg tagaggtccc acatggacag cgcgccccac
     6181 ttggaggtgg taatgcccgt gacgggcagg gtgta
//
LOCUS       DATALS010000290         6541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_212046, whole genome shotgun sequence.
ACCESSION   DATALS010000290 DATALS010000000
VERSION     DATALS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212046"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..2439
                     /locus_tag="VHS06_06425"
     CDS             379..2439
                     /locus_tag="VHS06_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987790.1"
                     /translation="MLRSVQIGLVAIFVLSACLVPSTAFATVPGEAPAATASGDTQAA
                     APAAPKEVRFGAVEAYTTPFRARESGVSWERAIYRWDQFQPSSAQDWKSNIWFPDSSL
                     AVELAEGRKVVGIVLGTPQWLAGPDPNAAFPNLNLPYDSPDNYWGQFMRRLVAQYAGR
                     IDDWIIWNEPDIWNNSANNQQFNGSVEQYYQMVKVAYQAAKSVNPKSRIVLAGLTYWW
                     DQAFGREQYFHRFLNVAKQDPTARANGFYFDVAAIHLYGNPQDLYDVTLLFKKMMREV
                     DLDKPVWINETNVVPHDDGGVVLGRDSYRASMEEQASYIIQAFACAMAAGDDRISVYK
                     MQDDTDRPGAEPYGLVRADEGAAPRPAYQAYQVVTNYMSGARSVRYFKQGSLAAIRIE
                     REKDWVTVLWDSSPTGIQAVLPARSPKALLVDKYGHGREALPKNGKYFVDLEGATANT
                     VPGAPAVYHIGGSPLLLVEGKVPGVTPYAPVQDWSAPGVNAGKSWVSQVTGYSVSGEW
                     LDYYRSHGDSDVLGHPLGSVRPDPANAKQYVQYFERGILEWHPENAAGQRVQKRLMGD
                     VFYPGYVEAAVDPQNAAQKPQGDVTFFPNQPGQGFGHYVANFASDGTPTHFREFFGSH
                     GGVESFGYPKEEPKLRNGRWTQRFQAGMLVAYPDAGKDGGSLVRLDTLGEKALGELTL
                     PMDW"
     gene            2735..3973
                     /locus_tag="VHS06_06430"
     CDS             2735..3973
                     /locus_tag="VHS06_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2987791.1"
                     /translation="MEVVHERCCGLDIHKKIVVACLMVSGGEGSPIKELRTFGTMTQD
                     LLALTDWLEGAGCTVVAMESTGVYWKPVYNLLEEHFELMLVNAQHIKAVPGRKTDVRD
                     AEWIADLLRHGLLKASFVPNREQRELRELIRFRRSLVEERASVLNRLQKVLEGANIKL
                     ASVVSSVDGRSARAMLEELVAGSTDTAAMAELAKGRLKEKIPELQKALEGKFGPHQRF
                     VVSELLAHLDYLEAAIERASVEVRERTRPFEEAVEQLDTIPGVGRETAEVIVAEVGTD
                     MSRFPNERHLAAWAGVAPGNNESAGKKKSSKARKGSKSLRTALVQAAQAAGRSKDNYL
                     SARYHRLAARRGNNRAALAVAHTILIIAYHLLNRHEEYRDLGPHYLDERNKESVQRRL
                     IRRLQDLGYKVSLDPIVEAA"
     gene            4149..4730
                     /locus_tag="VHS06_06435"
     CDS             4149..4730
                     /locus_tag="VHS06_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX2987792.1"
                     /translation="MAISASDLERLGIREKPEVVERLILEAMSGLLREMRPMDPGQDL
                     TAAEREALCKGGLTLDGRQLGKKDPILRTAVDYSALVASSLTVGLASEMLGVDESRIR
                     HRIAVRTLYGIRFRGGWRLPIFQFHGGDLLPGIEEVLPKVPRDLHPLELLNWFTSPNP
                     ELLLPGALEPVSPRDWLLAGGDPKVAARLAGEI"
     gene            4737..5363
                     /locus_tag="VHS06_06440"
     CDS             4737..5363
                     /locus_tag="VHS06_06440"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX2987793.1"
                     /translation="MARIADSPSSERLARVGPRIKRLPAGTELWRVYLFGGSHAGRWN
                     DFRPYGPVLSSRFDHHVPPPRPQDRAIMYAATEIATCLAEVFHGRKVIDRWRCSPWLV
                     GFSLQRELVLHDLSSDWLGAAGVDGDLNSGSHSRGQRWSHAIHDSYPEVQGLWYPSVR
                     HPDRAAIALYERAKPSLPVAPFFHRALSDPVLLIPLKMAAARLDLGLV"
     gene            5479..5892
                     /locus_tag="VHS06_06445"
     CDS             5479..5892
                     /locus_tag="VHS06_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987794.1"
                     /translation="MKGVGKQKGEGARQRSEAKHARRERRSGTSGRDARRTDRMDSSV
                     RRMIAYAGYISAVLLIMQSVNGGNAEPTIGYRWSPALLGAVLGVAVALIRNEMGLADR
                     LDALDAAKPGRLAYTLAWGLLGVAGIIGVMTLPRY"
     gene            complement(6200..>6541)
                     /locus_tag="VHS06_06450"
     CDS             complement(6200..>6541)
                     /locus_tag="VHS06_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987795.1"
                     /translation="AVFTLLSEWLTQGLTGVIVSERSMPNDGAIVVVVIALLVCWTLM
                     AVPAVILLERLRWKGPLRPVMGWVAASFFLWLAGAMMGGVGPLLFLLFWLAFGLAAGA
                     HFNPAPKRRQA"
BASE COUNT         1256 a         1895 c         2168 g         1222 t
ORIGIN      
        1 gtaggggcga cgcatgcgtc gcccaggcct ttcgctgtag ggcggcgtga ccgcgacgcc
       61 acgctcgcta ccgtcgcgtg ctcgctcgcg tcacgccgct gacccgcccg ttacccacca
      121 gcccgggcag gtcaggggcg acctgcccta ctaggggact tccctcgctc ctccgtgaag
      181 gagaatcgcc aaatgagggt tgtagaacgt cctcccgggt tgtaacctat agcggggcca
      241 gatctcggcc attccccagt ttgccgtcgg ccgcgaaccc tcttgccgat gcgacagcta
      301 tggtggtgct ttcctgtctc gacctcaggg cggatctttg gcccactctc ctctcgaggt
      361 aatgttgtct tgcggagtat gttaagaagc gtccagatcg gccttgtggc catattcgtg
      421 ctttcggcgt gtctagtccc ttcgacggcc tttgccactg tgcccggcga ggcgccggcg
      481 gccactgcat ccggtgatac ccaggcggct gctcccgccg ccccaaagga ggtcaggttc
      541 ggtgctgtgg aggcatacac cactcctttc cgagccagag agtccggggt tagctgggaa
      601 agggccatct atcgctggga ccagttccag cccagctccg cgcaggactg gaagagcaac
      661 atctggttcc ccgattcgag tttggccgtc gagttggcgg aggggcgcaa ggttgtgggc
      721 atcgtactgg ggacccctca gtggcttgcc ggtcccgacc ccaacgcggc tttcccgaac
      781 ctgaatctcc cctacgactc cccggacaac tactgggggc agttcatgcg ccggctggtg
      841 gctcagtatg ccggtcgtat cgatgactgg atcatctgga acgagccgga catctggaac
      901 aacagcgcca acaaccagca gttcaacggt tcggtggagc agtactacca gatggtgaag
      961 gtcgcttacc aggcggccaa gtcggtcaac cccaagagtc gcatagtgct cgccggcctc
     1021 acctactggt gggaccaggc attcggccgg gagcagtact tccatcgttt cctgaacgtt
     1081 gccaagcagg atccgacggc acgggctaac ggcttctact tcgacgttgc ggcgattcac
     1141 ctttacggca atcctcagga cctgtacgac gtcacccttc tgttcaagaa gatgatgcgt
     1201 gaggtggacc tggacaagcc ggtctggatc aacgagacca acgtggtgcc ccacgacgac
     1261 ggtggggtgg ttctcggcag agactcctat cgagcatcga tggaggaaca agcctcctac
     1321 atcatccagg catttgcttg cgcaatggcg gcgggtgatg atcgcatctc cgtctacaag
     1381 atgcaggacg acacggatcg gcccggcgcc gagccttacg gcctcgtgag ggcggacgag
     1441 ggtgctgcac cgcgtcccgc ttaccaggcg taccaggtgg tcaccaacta catgtccggt
     1501 gcgaggtccg tgcgctactt caagcagggt agcctggcag cgatcaggat cgagcgcgaa
     1561 aaggactggg tcaccgtact gtgggatagc tctccgaccg ggatccaggc cgtcttgccg
     1621 gccaggtcgc ccaaggcgtt gctggtggac aagtatggcc acggccgaga ggctttgccc
     1681 aagaacggta agtacttcgt ggatctggaa ggtgcaacgg caaacacggt tcccggtgcc
     1741 cctgccgtct accacatcgg gggcagtccg ctgttgttgg tagagggcaa ggtgcctgga
     1801 gtgaccccct acgctcctgt tcaagattgg tctgcgccgg gcgtcaacgc cggcaagagt
     1861 tgggtatcgc aggtgacggg ctactcggtt tccggcgagt ggctggacta ctaccgatct
     1921 cacggcgact cggacgttct gggacacccg cttgggtcgg ttcggcccga tccggcaaac
     1981 gccaagcagt atgtgcagta cttcgagcga gggatcctgg agtggcaccc ggagaacgcc
     2041 gccgggcagc gggttcagaa gcggctgatg ggggatgtct tctatccggg ctatgtcgag
     2101 gcggcagtcg atccacagaa tgcggcgcag aagcctcagg gggacgtgac cttcttccct
     2161 aaccagccgg ggcagggatt cggccactac gtggccaact tcgcgtccga cgggacgccg
     2221 acccatttca gggagttctt tggcagccat ggaggtgttg agagcttcgg ctatcccaag
     2281 gaggagccca agctgaggaa cgggcggtgg acgcaacgat tccaggcggg gatgctggtg
     2341 gcctaccccg atgcgggcaa agacggcgga tccctggttc gactggacac gttgggcgaa
     2401 aaggcactgg gcgagttgac gttgcccatg gactggtagc ccgctagcac gagaccatcg
     2461 gttttcgatc ttcgaggcgg tgatgttgac ttctaggagt tgcgcatgcc ccacgagggg
     2521 catcaccaat gatgaaaatg gctgaatagc ctgctaagct ccttggtgtt ggcgaggcat
     2581 aaggtcgagc cgggttggtg ctgtgagccc gtctaggagt ttgacgtggg tgtcctgagg
     2641 cagcaccgat tgttgccccc gaatggggag cacgtagatc agactctgaa ggatgaaggg
     2701 caccctgcct cgccacagca gggaggtcag caggatggaa gtcgtccacg agcggtgctg
     2761 tggcctggac attcacaaga agattgtggt cgcctgtctg atggtgtcgg gaggtgaagg
     2821 gagccccatc aaggaacttc gcaccttcgg cacgatgacc caggatctct tggccctgac
     2881 cgattggctc gaaggtgccg gctgcacggt ggtggccatg gaaagcacgg gggtgtactg
     2941 gaagcccgtg tacaacttgc tggaagagca cttcgagctg atgctggtca acgcccagca
     3001 catcaaggcg gtgcctggac gcaagacgga tgtgcgggat gccgagtgga tcgctgatct
     3061 gctgaggcac gggctgctga aggcaagctt tgttccgaac agggagcagc gggaacttcg
     3121 agagctgatc cgatttcggc gttctctggt ggaggagcga gcttccgtgc tgaaccgact
     3181 ccagaaagtg ctggaggggg cgaacatcaa gctggcttcg gtggtcagca gtgtggatgg
     3241 acgctctgcc agagccatgc tggaagagtt ggtggcagga tcgactgaca cggcagcgat
     3301 ggcggaactg gccaaaggga gactgaagga gaagatacca gaactccaga aggcgctgga
     3361 gggcaagttc ggaccccacc aacggttcgt ggtgtcggag ttgttggcgc acctggacta
     3421 cctggaggct gccatcgagc gagccagcgt cgaggtgagg gagcggacac gcccttttga
     3481 ggaagcggtt gagcaactgg acaccattcc tggggtgggc agggagacgg cagaggtgat
     3541 tgtggcggaa gttgggacag acatgagccg atttcccaac gagaggcact tggcggcatg
     3601 ggcgggagtt gcgcctggaa acaacgagag cgcgggcaag aagaagagca gcaaggcacg
     3661 caagggtagc aagagcctga gaaccgccct tgtccaagca gctcaagctg ccggcagaag
     3721 caaggacaac tatctctctg cccgctacca tcggctggct gctcgaaggg gaaacaaccg
     3781 agctgccctt gctgtcgccc atactatcct gatcatcgcc taccatctgc tcaaccgcca
     3841 tgaagaatac cgggatctgg gtccccacta cctggacgag cgcaacaagg aatctgtcca
     3901 acgtcgtctc atcaggcggc tccaagacct gggctacaag gtctcactcg atcccatcgt
     3961 ggaggccgcc taacgagtta cgttctattt tcagatcagc ccgggtcgtc cctgacccgg
     4021 gcggctctgc tctccatcgt cggggttggg acaaccccga ctcctagtgt tagggtattc
     4081 agatcgccac ggctctcacg cgtacgctgg cgctcggtat gcgatagagc gcagcggagg
     4141 tgattgccgt ggcgatctct gctagcgacc tggagaggtt ggggatacga gagaagccgg
     4201 aggtagtgga gcggctgata ctggaggcga tgtccggctt gcttcgcgag atgcggccga
     4261 tggatcccgg acaggacctc acggccgccg agcgggaagc gctgtgcaag ggcgggttga
     4321 cgctggatgg tagacagctg ggaaagaagg atcccatcct gaggacggcg gtggactatt
     4381 ctgccctggt agcttccagt ctgaccgtcg ggttggcctc ggagatgctg ggtgtggatg
     4441 agagccggat ccgccatagg attgccgtgc gaaccctgta cggcatcagg tttcgaggag
     4501 ggtggaggct tccgatcttt caattccacg gcggtgactt gcttccagga atcgaggagg
     4561 ttctcccgaa ggtccctcgc gatctgcacc ctctggagtt gctgaactgg tttacctcgc
     4621 caaaccccga gttgctgttg ccgggtgcgc tcgagcccgt cagtcccagg gactggctgc
     4681 tcgccggagg cgatccgaag gttgccgcac ggctggcagg ggagatctag cgccccatgg
     4741 cccgaatagc cgactcaccg tcctccgagc ggctggctcg agttgggccg agaatcaaga
     4801 ggttgccggc gggaacggaa ttgtggaggg tgtacctctt tggcggcagc cacgcgggcc
     4861 gctggaacga cttcagaccc tacggccccg tactatcgtc gcgcttcgat caccacgtcc
     4921 ccccgccccg cccgcaggat cgggccatca tgtacgcggc gactgagatc gcgacctgcc
     4981 tggctgaggt gttccacgga aggaaggtca tcgatagatg gcgctgttct ccgtggctgg
     5041 tcggattctc actgcaaaga gagttggtgc tgcacgatct ctccagcgat tggctggggg
     5101 ctgccggcgt ggacggcgac ctcaactccg ggagccactc cagggggcag agatggtctc
     5161 atgcgattca tgatagttac cctgaggtgc agggactctg gtatccatcc gtgagacacc
     5221 ccgatagggc agccatcgca ctgtacgagc gcgccaagcc ctccctcccc gttgccccat
     5281 tctttcacag ggcgttgtcc gaccctgtgc tgctcatccc gctgaagatg gccgctgcca
     5341 ggctggacct cgggttggtg tagcgatcag cggacaactg ctgccggctc gtctttcgtc
     5401 aactactatc ttcctgtggg acttgtgtcg tgctatcctt cccgccaacc acgatggcag
     5461 ccgaaggggg cccgaacaat gaagggcgtc ggaaagcaga agggcgaggg tgcccggcag
     5521 aggagcgagg cgaagcacgc acggagggag cgacgctccg gcacgtccgg acgcgacgcg
     5581 cggaggacag accgtatgga tagcagcgtg cggcggatga tcgcctacgc tggctacatc
     5641 tcggcagttc tgttgataat gcagagcgta aatggcggca acgcggagcc gacaatcggg
     5701 taccgatgga gtccggcgtt acttggggcg gttctcggcg tagccgtcgc gcttatccga
     5761 aacgagatgg ggttggccga taggctggat gccctggacg cggcgaagcc cggccggctt
     5821 gcctacaccc tggcctgggg actcctcggc gtcgccggga taatcggggt gatgacgcta
     5881 ccgcggtact gagagggatc ctggagtgcg gcggctcgac gccgcaacgc tacccggcac
     5941 atcctgccgg ctacggagac gcccctctca ttccgggagt ggggcatact gtgtggcgcg
     6001 gtttcgttgg gtggagagag acgccgtgat catgattgta gggcggggtc ttgtgccccg
     6061 cctcccttgg cgtggtcacg accgcccaac gattttctgc cgacgctatt ggcggggcac
     6121 aagaccccgc cctacgagag ttaccaagtg gcttcaacgt ggtgggcgat caccaaccgt
     6181 gcctgtaaga agcgaccggt tacgcctggc gccgcttggg cgcagggttg aagtgtgctc
     6241 ctgctgccag gccgaaggcg agccagaaca gcaggaaaag cagcgggcca accccaccca
     6301 tcatcgcccc cgccagccat aggaagaacg aggccgccac ccaccccatc accggtcgca
     6361 gagggccctt ccatcggagt cgttccagca gaatcacggc ggggacggcc atcagcgtcc
     6421 agcataccag cagcgcgatc accacgacga caatggcgcc gtcgttgggc atcgatctct
     6481 cagagacgat tacccctgtg agaccttgag tcagccactc cgagagtagg gtgaatacgg
     6541 c
//
LOCUS       DATALS010000291        14188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_164154, whole genome shotgun sequence.
ACCESSION   DATALS010000291 DATALS010000000
VERSION     DATALS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 14188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14188
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164154"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..852
                     /locus_tag="VHS06_06455"
     CDS             358..852
                     /locus_tag="VHS06_06455"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX2987796.1"
                     /translation="MKAAELLGMPVISIAGARKLGSVQDVVLNPAEQKVVAIRVKLAQ
                     EGIVRTVPASEITVGRDAITIPELKSGAEDESLGGKGAVDLGKLRGKKALSHDGNVLG
                     SVDDVVIDGSDMSIKGYEISKGVLSGLFGGKSHLPSVKGFNYGRDILMVPDAAISQEH
                     RRAA"
     gene            1036..1788
                     /locus_tag="VHS06_06460"
     CDS             1036..1788
                     /locus_tag="VHS06_06460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX2987797.1"
                     /translation="MRLAGKRALVTGGTRGMGRAIAVGFAREGADVAIFYRQSEEAAR
                     EVVEEIRKLGRRGIALRVDTSSRSQVEAGVASVLSEFGELDVLVNNAGIATRCHFLEL
                     TDEEWDRVHSTNLRGYFIVGQTVARHMADRRSGVIINVSSLNQQKARRNRSHYLASKG
                     GVLMLTKGMAVDLAPYGIRVNVLAPGTIETDINRQDLADPAFRRERESNILLGRLGRP
                     EDIVGAALFLASDDSSFVTGASLLVDGGATAV"
     gene            1916..3310
                     /locus_tag="VHS06_06465"
     CDS             1916..3310
                     /locus_tag="VHS06_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2987798.1"
                     /translation="MLRSDLLAELGHIVGVDNIFYSRRDRLSYEYDGTAVTSQPDVVL
                     FPRDSHDTSAIMRVARREGIPVVPRGAGTNLSGGTVPLEGGIVVCFTRMNRILHFDTG
                     DERAVVEPGVVNLDLEGAVAPFGYLYAPDPSSQKVSTMGGNIAENAGGPHCLKYGVTT
                     NHVYGLEVVLPDGQIVQTGGAVEDCPGYDLTGLIVGAEGTLGMVTSITVRLLRQPEAI
                     RTMLAIFDDLDSCGDAVSDIIAQGIIPATLELMDSTTVGAIEDSGKAAGYPRDADGIL
                     LIELDGMRDGQDRQIQQIREICRAHNAREVQVAKTDADRDRLWSGRRAAYSALVALDS
                     GSSIHDTTVPRTNMTEALHRIREIGNRHRVRVSETGHAGDGNLHPLIVYNLADPDEVE
                     RVRRADHEIVELCVEMGGSPTGEHGIGREKIAYMSAVYSEVDLELMRMAKRAIDPNGL
                     LNPGKLIPPASPRS"
     gene            3448..4839
                     /locus_tag="VHS06_06470"
     CDS             3448..4839
                     /locus_tag="VHS06_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEX2987799.1"
                     /translation="MSGSADPLQRIAALGPVEPYRVKMVEPIRTSTREERERWLAEAG
                     LNLFRVPAECVFIDLLTDSGTGAMSHHQWSGLMQGDESYAGARSFFRFRDTVRELFGL
                     PEVIPTHQGRAAEHLLFSELVRPGKRVISNTFFDTTRANAEAAGAVADDLPSPDARDL
                     GSEQPFKGDIDLNRLADVLKRAPAGEIVGVVMTVTNNAGGGQPVSMANLRGASELCRS
                     YGIPLILDASRIAENGFFIRERERGYADKTVKEIVLAMGECADIMTMSAKKDGLVNIG
                     GLLALRDHDLAERIRRQMVVTEGFPTYGGLAGRDLEAMAVGLREAQDEDYLAHRIGQV
                     RLLADLLRDLGVPIIEPPGGHGVFVDAAGFLPHIPRSRYPGQALAVALYLEGGVRSCE
                     IGSVMFGDDPDARWPELVRLAIPRRTYSNAQLGVVASSFASLRERRSSIRGVAIVDAP
                     PVLRHFTAGFAFV"
     gene            complement(5046..6815)
                     /locus_tag="VHS06_06475"
     CDS             complement(5046..6815)
                     /locus_tag="VHS06_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX2987800.1"
                     /translation="MIRYTIREKRKGPGCWLPATVLVLVLVAATMSLNLGDLRSRFVS
                     SLGDDIRNEITALYSSLTMRHVETADMVPIRYTGMNPMGVNTFLEQEVEIAKRRQTLQ
                     MIKDAGIGFIRQQFPWEDIERPTKGRFWDEKYLKSSWEKYDNIVDLANEYGIQMIVRL
                     DLPPKWAHPNNEWPHTPPENLSDYGDFVYSVVSRYKGKVKYYQLWNEPNLAIEWGGKE
                     VNANDYVQLLKIGYQRAKEADPDAVIISAALAPTIEESWQALNDQIYLQEMYDAGAKD
                     YFDILSVMAYGLRFGPDDRRLALKDVNFSRPLLIREIMVKNGDASKPIWASEMGWNAQ
                     PDSTTAEPVFGRVSEALQARYTVRGFQRAAQEWPWMGVMNIWFFKRADDHEVDQPMYY
                     FRLVNPDFTPQPVYEAVKALGAEPAILHRGFVQEDHRALVFQGEWKMVSDPQASLGSM
                     KSTAQAGATISAQFEGTSISLVVPEGPEWGQAYVEIDGSPQRANLLPKDGSARATVDL
                     ASPEKRWQQQIPVASDLSGGTHQLVIKTGGPFALDGIVVDSPPPWWAQWLVPAIGGAA
                     AVLLVFLLALVRSQIANRGRLRL"
     gene            complement(6931..7224)
                     /locus_tag="VHS06_06480"
     CDS             complement(6931..7224)
                     /locus_tag="VHS06_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX2987801.1"
                     /translation="MIVGACRIVLHLPDAHSLKEKRHVVKSIIARLHNQFNVSAAEVE
                     SQDLWQQAVIGVAVITNDTQHANEVLSTAVNFVEGATPEAEMTDYQIEITPVL"
     gene            complement(7256..8470)
                     /locus_tag="VHS06_06485"
     CDS             complement(7256..8470)
                     /locus_tag="VHS06_06485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2987802.1"
                     /translation="MRILFLTPQLPFPARQGTQMRNLQIIQAAARAHQVDLVSFARKG
                     ETVDADSPLAKLCGEIRTVPAPTTRGRKSRLRDLALSPEPDLAFRLRSPEFESALRFM
                     LSVWKYDAVQVEALEMATYLPVVRLFAPHAAVIFDAHNCEYLIQARAAAVDARLPSTW
                     PKAAYSLIQCGKLKEYEAAVCRRAGLVLAVSQSDADTLYNLGLSARPLVVPNGVDASS
                     YRPDLVLHPEHDSLLFTGTMDYRPNVDAVEWFAKKVLPIIVRKRPGVTLRIVGRAPSP
                     RVMALAEDPMIEVTGEVDDVRPYFSRSSLFVVPIRMAGGARLKILEAMAMGLPVVSTT
                     MGAEGIDLLDGEDALLADDADGFADAVVKLLEDPAFAGKLAFNGRRLVEYQYDWEQVA
                     PLLLKSYSSIGA"
     gene            complement(8467..9573)
                     /locus_tag="VHS06_06490"
     CDS             complement(8467..9573)
                     /locus_tag="VHS06_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX2987803.1"
                     /translation="MRIAIDARLISYAQGGISQYTIRLVRALAELGSGDELLLLESRR
                     GIEDVQWPESVRHVPMVTPPHNRFEQITLPMELMRVRPDLIHNPDFIPPFRRNCRSVI
                     TIHDLAFIRYPHLLTDESARYYGQVKRAVVSADQIIAVSHSTAQDILELLDADPAKVN
                     VVYSAAAPDCRPLTPEQVRHYRSLLGLPERYLLFVGTLEPRKNLPTLLEAFAPVWQRH
                     GVPLLVVGGKGWLYQAVFETRDRLGLEEGVGFVGAVSSNQLPYYYGCADCLVMPSLYE
                     GFGLPALEAMACRTPVIVSNVSSLPEIVGDAGLTVDPTDIDGLTKALERVVTDNELAT
                     RLGRLGQERAAQFSWQKTAAETLAVYRKAAEVTT"
     gene            complement(9743..10612)
                     /locus_tag="VHS06_06495"
     CDS             complement(9743..10612)
                     /locus_tag="VHS06_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2987804.1"
                     /translation="MTTASAKTRSVRLGDIDLALISDGLMRVDGGGMFGVVPRQMWEA
                     KHAPDELNRVAIVANCLLIRDGEHTIVIDTGIGSKLRPRTMSIMGIESAGELPVLLAE
                     QGVKPADVDIVINTHLHFDHAGGNTLLQSGRAIPTFPNATYIAQRGEWEDATHPNERT
                     RASYLAENLLPVWETGRLELIEGDTQITPHVRCVLTPGHTPNHQSILIESGEGERALY
                     LADVAPLAVHMERLTWLAAYDLEPLRTMESKRRLQRQVVEDHTLLIFPHDPEIGMGYL
                     RADSGRFWVDGYK"
     gene            complement(10609..11370)
                     /locus_tag="VHS06_06500"
     CDS             complement(10609..11370)
                     /locus_tag="VHS06_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2987805.1"
                     /translation="MELVVMGSGAAYPGPHQASSGYLIRDGQTGLLVDCGNGVVSKLQ
                     EAGEMDNVTALLFSHLHADHLLDIFPLFYARLYGKGKVYPTLPVFMPPGEVERFARLA
                     EVLRVDTRKLCEQVFSVREYDPAGPLSVDALSISFLKTVHPVPAYALRIEDGRGSMVF
                     SADTGPLPELADFARGCDLLLCEASIGPEDFDPDHAIHLTPAMAAEVAASAGARQLLL
                     THIWPFYDRQEMLQQAKAVFPQAELAEELNRYQIA"
     gene            complement(11525..12937)
                     /locus_tag="VHS06_06505"
     CDS             complement(11525..12937)
                     /locus_tag="VHS06_06505"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX2987806.1"
                     /translation="MKEYSNRFIIFLFVADIFFTELALFLAEITRGVMPFGRESIGMS
                     FLDLYILLAVAIFWSFFLRLFGAYDPRCLSSIVDEGRAVALATAAGLLTLASFFYLFN
                     VEFRSRMLFTYFGAIDLALLLNYRLATRYVVKSMVAGGYYVRRVVLVGSTKVGKELAY
                     IIGSQPWSGLSVVGFVDDDPELQGKEIEGRPVLGTTNDLTHVIEQNSVHDVIVALGGE
                     EHDKIAQIILSLQSYPVRVRVVPDLFEMVSVRAQVEDFWGIPLIGLRDPVITGFDRAF
                     KRAFDVVLSSALLLILGPVMLAIALAIKTDSKGPALFKQRRVGENGRLFWMYKFRTMV
                     NGADKMVAKLESDGVYADGVYKVKEDPRVTRVGKILRRMSADEFPQLFNVLKGDMSLV
                     GPRPEQPWIVERYQPWQRKRLSVMPGMTGWWQVNGRSDRPMFLNTEYDLYYIQNYSPV
                     LDLLILWKTIWVVLRGKGAY"
     gene            complement(12961..14085)
                     /locus_tag="VHS06_06510"
     CDS             complement(12961..14085)
                     /locus_tag="VHS06_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2987807.1"
                     /translation="MEATIGGTKRHLLALIRGLDRNQFEVEVAAPEIRSEAFDDVTFT
                     KEVREAGIKQHFVPMQRAISPGSDLRSFSQLVALMARGRYDLVHTHSSKAGFLGRFAA
                     RAAGVKVTVHTPHGFYFLNQKPGRKRSFYLQLERMAGLTTSRIIALSPTEMAEAVDNG
                     IIKSDRIALIENGIRVPAVPSDGIVARLRETLAPEAEYLVGTVARFTPQKGPFDFVRM
                     TSHLVQLVPGVHVLWCGDGELREQVESLAGELGVSDRLHFLGYRTDVLDIMSTLDLFV
                     LTSLWEGLPYTVLDAMALGKPVVATAAVGTRDIVQEGVTGLLTPVGEPAAAAEAIARL
                     LRAPAERQAMGTAGHETILRRFSQEAMVKRTGQLYVDLVG"
BASE COUNT         2591 a         4272 c         4596 g         2729 t
ORIGIN      
        1 cgactgtcgt cggagcacct ccgtcatatc ggtcgcgggg cgcagcgcca ccgccttctc
       61 gtggctgact gagaaggagg tgtggccggc caaccggtcc agaaccttgt tcagttccag
      121 agtatgcagg tagtgttcgt ccatagtagt catcgctaat cagcacggta gcttctcccg
      181 gctgagagaa gctaccctct cacctcaacg tacgttagtt caattttacc gcatcgtggc
      241 tgcctgttgc aggggccgtg gttcttcgaa cggctggcac atgcaatgcc cagtctccgt
      301 ctcagcgtcg ggggtacccg cgcgccgacc gactacggag aggggagggt tcgctagatg
      361 aaggcagcgg agttgctggg aatgccggtg atcagcatcg ctggagctag gaagctcggg
      421 tccgtccagg acgtcgtgct gaatccggcg gagcagaagg ttgtggctat cagggtcaag
      481 ttggcgcagg aggggatcgt taggaccgtt ccggcttccg agataacggt tgggcgcgat
      541 gcaatcacga tcccggagtt gaagtccggc gccgaggacg agtcgcttgg gggcaaggga
      601 gcggtcgatc tgggcaagct gcggggcaag aaggcgttga gccacgacgg caacgtcctg
      661 gggagtgtcg acgatgtcgt gatcgacggc tccgacatga gtatcaaggg gtacgagatc
      721 agcaagggcg ttctgtccgg actctttgga gggaagagcc acctaccctc cgtcaagggg
      781 ttcaactacg gcagggatat tctgatggtg cccgacgcgg cgatctccca ggagcatcgc
      841 agggcggcgt gaatcctggg cgatccggca tcttctactt gccgtgcgcg cgagggcgct
      901 gtaggatcag ggcgactggc gaaggtgtaa tccgtgccat tttcccccga cagggcgctg
      961 atgtgttccc tgttgccgcg attggttatc tccgggacat ctctacaggc gagttttgag
     1021 gtggagggga ttgtcatgag gttggccggc aaacgtgccc tggtcaccgg cgggaccagg
     1081 ggcatggggc gagccatcgc cgtgggtttt gcgcgggagg gcgccgacgt tgccatcttc
     1141 taccggcaga gcgaagaggc cgcgcgggag gtggtggagg agattcggaa gctggggcgt
     1201 cgtggtatcg cgttgcgcgt cgacacctct tctcggtccc aggtggaagc gggcgtagcc
     1261 tctgtcctgt ccgagttcgg ggagctggat gtcctggtca acaatgcggg tatcgccacg
     1321 cgctgccact ttctggagtt gaccgacgaa gagtgggaca gggtccactc caccaacctg
     1381 agaggctact tcatcgtggg gcagacggtg gcccgccata tggcggatag gcgctccggg
     1441 gtcattatca acgtatcctc cctgaaccag cagaaggcgc ggcgtaatcg ctcccactat
     1501 ctcgccagca agggcggtgt gctgatgctg accaagggca tggctgtgga tcttgctccc
     1561 tatgggattc gggtgaacgt cctggcgccg ggtaccatcg agacggacat caatcgccag
     1621 gacctggcgg atccggcctt tcggagagag cgcgagtcta acatcttgct gggccggctg
     1681 ggcaggccgg aggacatcgt cggcgcggcc ctcttcctcg cctcggacga tagttccttc
     1741 gtcactggag ccagcctgct ggtggatggg ggcgcaaccg cggtatgatt ccctttccgt
     1801 gggggcgatg gacgtattgt ccccacggat gccgccgata actctccttc cgtagtacaa
     1861 tcctttcggc gggcattgat gccggccgaa agcttctggg cgttggagac cgagtatgct
     1921 gcgtagcgac ctgttggcgg agttgggcca cattgtcggc gtcgacaaca tcttctactc
     1981 acgacgtgac cgtctgagtt acgagtacga cggcaccgcc gtgacctcgc agcccgacgt
     2041 tgtgctgttc cccagggata gccacgacac ctccgcgata atgcgggtgg ctcgccgaga
     2101 ggggatcccc gtggtaccca ggggagccgg caccaatctc tccggcggca cagtccccct
     2161 ggaggggggc atcgtcgtct gttttacgcg catgaaccgg atactccact tcgacaccgg
     2221 cgacgagagg gcggtggtcg agccgggtgt cgtgaacctg gacctggagg gcgcggtggc
     2281 ccccttcggg tacctgtacg cgccggaccc ctccagccag aaggtctcca cgatgggtgg
     2341 caatatcgcc gagaacgcgg gcgggccgca ctgcctaaag tacggcgtca caaccaacca
     2401 cgtttatggc ctggaggtgg tactgccgga tggacagata gtgcagaccg gcggggcagt
     2461 ggaggattgc cctgggtacg acctcaccgg cctgatagtc ggcgctgagg gcaccctggg
     2521 gatggtaacc tctatcaccg tgaggttgct gcggcagcct gaggcgatcc gcaccatgct
     2581 ggccatcttc gacgacctgg attcctgcgg agacgccgtg tcggacatta tcgctcaggg
     2641 gatcatccct gccactctgg agttgatgga tagcacaact gtgggggcaa tcgaggacag
     2701 cgggaaggcg gctggctacc cacgggatgc ggacggcata cttctgatcg aattggacgg
     2761 catgagggat ggccaggatc ggcagatcca gcagataagg gagatttgcc gcgcgcacaa
     2821 cgcccgcgag gttcaggttg cgaagaccga cgccgacagg gatcggcttt ggtcggggcg
     2881 tcgcgctgct tacagcgccc tggttgccct ggactccggg tcctcgatcc acgacaccac
     2941 ggttccacgg accaacatga cggaggcgct gcaccggatt cgggagattg ggaacaggca
     3001 tagggtgcgg gtctccgaga ccgggcacgc gggcgacggc aacctacacc ccctgattgt
     3061 ctacaacctc gccgacccag acgaggtaga gcgggttcgc cgtgccgacc acgagatcgt
     3121 ggagctgtgt gtggagatgg gtggctcccc taccggcgag cacggcatcg gccgcgagaa
     3181 gatcgcctac atgtcggcag tgtactcaga agtggacctg gagttgatgc ggatggccaa
     3241 gcgggccatc gacccgaacg ggctgctcaa cccgggtaag ctgataccac ccgcatctcc
     3301 tcgctcgtag atgtcagtag cgattttgga ttgacagccc cactggcaag gtctagcatc
     3361 ccgcctggct aagtaaatgc cggaagaacg ctcggttctg gtccggcagg ccgctgcctg
     3421 cactacttaa ttcgcgaggt gttaagtatg tcgggatccg ccgatccact tcagaggatc
     3481 gccgcactgg gacctgtcga gccgtacagg gtgaagatgg ttgagccgat ccggaccagc
     3541 acccgcgagg agcgggagcg ttggttggcc gaggccgggc tgaacctctt ccgcgttcct
     3601 gccgagtgcg tcttcataga cctgctcacc gattccggca ctggagctat gagccaccac
     3661 cagtggagcg ggctgatgca gggcgacgag agctacgcgg gtgcgcgcag cttctttcgg
     3721 tttcgggaca ccgtgaggga actgttcggg ctgcccgagg tgattcctac ccatcaggga
     3781 agggcagcgg aacacctcct gttctcggag ttggtcaggc cgggcaagcg ggtcatctcc
     3841 aacaccttct tcgatacgac gagggcgaat gccgaggctg caggtgccgt cgccgacgac
     3901 ttgccctccc cagatgcccg agacctgggg agcgaacagc ccttcaaggg cgatatcgac
     3961 ctgaaccggc tggccgatgt gttgaagcgt gcgcccgccg gagagatcgt cggtgtggtt
     4021 atgaccgtca ctaacaacgc aggcgggggc cagccggtgt ccatggctaa cctgcgtggc
     4081 gccagcgaac tgtgccggag ctacgggatc ccgttgatcc tggatgccag ccgtatcgcc
     4141 gagaacggct tcttcatccg cgagagggag aggggatacg ccgacaagac ggtcaaggag
     4201 atcgtcctcg ccatgggtga gtgcgccgac atcatgacca tgagcgccaa gaaggatggc
     4261 ctggtgaaca tcggcgggct gttggcgctg cgggaccacg acctggccga gcggattcgg
     4321 cggcagatgg tggtgacgga gggtttccca acctatggcg gcctggccgg tcgagacctg
     4381 gaggccatgg cggtcgggct acgggaggct caagacgagg actaccttgc ccatcgaatt
     4441 ggacaggtgc gtttgttggc tgatcttctg cgggatctcg gcgtgccgat catcgagccg
     4501 cccggcgggc acggtgtctt cgtggatgcg gctggattcc tgccgcatat cccgcggagt
     4561 cggtacccgg ggcaggcact ggctgtggcg ctctacctgg agggtggggt gcgaagctgc
     4621 gagatcggct cggtgatgtt tggagacgat cccgacgccc ggtggccgga gttggtgcga
     4681 ttggcgatcc cgaggcgtac ttactccaac gcacaactgg gggtggtggc gtccagcttc
     4741 gcgtcgctgc gggagcgacg gtcatcgatc cgtggtgtgg ccatcgtgga tgcgccgccg
     4801 gtgctgcgcc acttcacggc cggcttcgcc ttcgtgtaga tatggagtgc ggcgtgtaaa
     4861 cgccgctctt ccgtgtggaa tgcgaacggc gcgtaagcgc cgcactccga agcgggccgg
     4921 cgtggggtaa aaggaggcgg tgccgtgaga cctctatgag tgagggccgc gccacccacc
     4981 atggggcgct tacgcgccct gcctagacgc tgctggaaag cggtgctcac gcaccgcact
     5041 ccacatcaca gcctcaggcg tccgcggttc gctatctggc ttcggaccag cgccagcagg
     5101 aacaccagga gaacggcggc tgcccctccg atggccggta ccagccactg cgcccaccat
     5161 gggggagggg agtccacaac gatcccgtcc agggcgaagg gaccgccggt cttgatgacc
     5221 agttggtgag ttccgcccga aaggtccgag gccaccggta tctgttgctg ccatctcttc
     5281 tcgggagacg cgagatcgac cgtcgccctt gcggagccgt ctttgggcag cagattggcc
     5341 ctctgcgggg agccgtctat ctcgacataa gcctgccccc actcggggcc ctccggcacg
     5401 acaagggata tagaggttcc ctcgaactgt gccgaaatcg ttgcccccgc ctgggccgtc
     5461 gatttcatgg agccaagact ggcttgaggg tcggacacca tcttccactc gccctggaac
     5521 accagcgctc gatggtcctc ctggacgaag cctcggtgga ggatcgccgg ctcggccccc
     5581 agcgctttca ctgcctcata cactggttga ggagtgaagt cggggtttac gagacggaag
     5641 tagtacatcg gttgatcaac ctcgtggtcg tcggcccgct tgaagaacca gatgttcatg
     5701 actcccatcc agggccactc ctgggcggcc cgctggaagc ccctcacggt gtacctggct
     5761 tgaagggcct cgctcacgcg accgaacacg ggctcggccg tcgttgaatc cggctgggcg
     5821 ttccagccca tctcggaggc ccagatcggt ttggaggcat cgccgttctt caccatgatc
     5881 tcgcgaatca agagcggtcg cgaaaagttg acgtctttca gcgccagccg gcgatcgtcc
     5941 ggaccgaacc gcagcccgta ggccatcacg gagagtatgt cgaagtagtc cttcgccccg
     6001 gcgtcgtaca tctcttgcag gtagatctgg tcgttcagcg cctgccagct ctcctcgatg
     6061 gttggggcca gcgctgctga gatgatcacg gcatcggggt ctgcctcctt tgccctctgg
     6121 tacccgatct tcagcagttg gacgtaatca ttggcgttga cctctttgcc gccccactca
     6181 atggctaggt tcggctcgtt ccaaagctga tagtacttta ccttgccctt gtaccggctg
     6241 acgacgctgt agacgaagtc gccgtagtcg ctcaggttct ccggcggggt gtggggccac
     6301 tcgttgttgg ggtgcgccca cttgggtggg aggtccagcc gcacgatcat ctgaatgccg
     6361 tactcgttgg ccagatcgac gatgttgtcg tatttctccc aactgctttt gaggtacttt
     6421 tcgtcccaga atctcccctt ggtaggacgc tcgatatcct cccatgggaa ctgctgtcga
     6481 atgaagccga tcccggcatc cttgatcatc tgcagcgtct ggcgtcgctt cgctatctcc
     6541 acctcctgct ccaggaaggt gttgacgccc atcgggttca tgccggtgta gcggatggga
     6601 accatgtcgg cagtctcgac atgccgcatg gtgagactgg agtagagtgc ggtaatttcg
     6661 ttgcgtatat cgtcaccaag ggaactgaca aatctcgacc tcaggtcgcc gagattgagt
     6721 gacatggttg ccgctaccag aacaagcacc aataccgtcg ccggcagcca gcatccggga
     6781 ccctttcgct tttctcgaat ggtgtatcga atcatcggag gttgcactct ccgtggaaag
     6841 ctggaagcag aacggggcgc agaacaggga atgcgcaatc cctactttac acgcttagag
     6901 ggcgtgtggg aataggggaa cggtggacac ctatagaacc ggggtgattt cgatctggta
     6961 gtcggtcatc tcggcttccg gggtcgcccc ctccacgaag ttgaccgctg tggagagcac
     7021 ttcgttggcg tgctgcgtat cgttggtgat taccgcgacg ccgatcacgg cctgctgcca
     7081 gagatcctgg ctctcgacct cggcggcgga gacgttgaac tggttgtgca gcctggcgat
     7141 gatagacttg acgacgtgcc gcttctcctt gagggagtga gcatccggaa ggtggagtac
     7201 tatcctgcag gctccgacta tcacttcttc ccccttttcc ggcgggccta cgatctcacg
     7261 cccctatgga agagtatgac ttgagcaaga ggggggccac ctgttcccag tcgtactgat
     7321 actccacgag ccgcctgccg ttgaaggcca gcttgccggc gaatgccgga tcctccaaca
     7381 gcttcaccac cgcgtcggcg aagccgtccg cgtcgtcggc cagcagcgcg tcctcgccgt
     7441 cgagcaggtc gatcccctcc gctcccatgg tggtggaaac cacgggaagc cccatggcca
     7501 ttgcctccag gatcttcagt cgggctccac cggccatgcg gatggggacc acgaagagcg
     7561 aactgcggct gaagtaaggg cggacgtcgt ctacctcacc ggttacttcg atcatcggat
     7621 cttcggccag cgccatcacc ctgggcgatg gtgcccgccc gacgatgcgc agggtgactc
     7681 ctggtctctt tcggacaatt atggggagaa ccttctttgc gaaccactcc acggcatcga
     7741 cgttggggcg gtaatccatg gttccggtga agagcagact gtcgtgctcc ggatggagca
     7801 ccaggtcggg acgatagctg gaggcatcaa cgccgttggg taccaccagg ggacgggccg
     7861 aaagcccgag gttgtacagc gtgtcggcat cggactggga gacggccagt accagccctg
     7921 cgcgcctgca aacggcggct tcgtactcct tcagcttccc gcactggatc agggagtagg
     7981 cggctttcgg ccacgtcgag ggcaggcgag cgtctaccgc cgcggctcgt gcctggatca
     8041 ggtactcgca gttgtgggcg tcgaagatca ccgcggcgtg tggcgcaaac agccgcacga
     8101 ccggcaggta ggtcgccatc tcgagcgcct cgacctgaac ggcgtcgtac ttccagaccg
     8161 agagcatgaa gcgcagggcc gattcgaact ccggtgaccg gagccggaat gccaggtcgg
     8221 gctcaggcga gagcgcaagg tcgcggagcc ggctcttcct tccacgggtt gtgggtgccg
     8281 gtaccgtccg tatctcgccg cacaacttgg ccaggggtga gtcggcgtca accgtttctc
     8341 cctttctggc gaaggagacc aggtcgacct ggtgagctct ggcggcggcc tggatgatct
     8401 ggagattgcg catctgagtg ccctgccgcg ccgggaaggg cagttgtggg gtcagaaaga
     8461 gtatcctcaa gtcgtgacct ccgccgcttt gcggtacacc gccaacgtct ccgcggcggt
     8521 cttctgccag gagaactgcg ccgccctctc ctgtcccaat cggcccaacc tggtcgcaag
     8581 ctcgttgtcc gtcacgaccc gctccagggc cttggtcagc ccatcgatgt cggtggggtc
     8641 caccgtcagc cccgcgtccc ccacgatttc ggggagggaa gagacgttgg agacgatcac
     8701 cggggtgcgg caggccatag cctccagcgc agggaggccg aagccctcgt acagcgaggg
     8761 cataaccagg caatcggcgc agccgtagta gtagggtagc tggttggatg aaaccgcgcc
     8821 tacgaagccg acaccttcct ccagtcccag cctgtcgcgg gtctcgaaca ctgcctggta
     8881 cagccagcct ttgcccccga cgaccagcag cggtactcca tggcgctgcc agactggggc
     8941 aaacgcttcc aggagggttg ggaggttctt gcgcggctcc agcgtcccga cgaacagcag
     9001 gtagcgctcc ggtagcccga gcagggagcg gtaatggcgc acctgctcgg gggtcaacgg
     9061 acgacagtcc ggagcagccg ccgagtaaac cacgttgacc tttgccggat ctgcatccag
     9121 caactccagg atatcctggg ccgtggaatg cgatacggcg atgatctgat ccgcgctcac
     9181 gaccgcgcgc tttacctggc cgtagtagcg agcactctcg tcggtcagca ggtgcgggta
     9241 ccggatgaac gccaggtcgt ggatggtgat gacggagcga cagttcctcc ggaagggagg
     9301 gatgaagtcg gggttgtgta tcaggtccgg ccgcaccctc atcaactcca tgggcagggt
     9361 aatctgctcg aagcgattgt ggggaggggt gaccatgggg acgtgccgga ccgattccgg
     9421 ccactggaca tcctcgatcc cccttcggct ctccaacagc agcagctcgt ccccggagcc
     9481 aagctccgcc agggcgcgca ccagccgtat cgtgtactgg ctgatcccgc cctgggcata
     9541 tgagatgagt ctggcgtcta tcgcaatgcg catggaactc cccgatcggc ggatgacaat
     9601 ggactattgg cgtggatggt ccttgcccag ggtagggcgg tcacctggga catatcgtag
     9661 ggcggggtct tgtgccccgc ccagagcgtt accattatta ccgtcgtggt cgcagccacg
     9721 acgtgggagg cggggcacaa gactacttgt acccatccac ccagaaccgc cctgagtcgg
     9781 ctctcaggta gcccataccg atctcgggat cgtgtgggaa gatcagcagc gtgtggtctt
     9841 ccaccacctg cctctgaagc cgccgtttgc tctccatggt gcgaagcggc tccagatcgt
     9901 aggcagccag ccaggtcagc cgctccatgt ggacggccag aggtgccacg tctgccaggt
     9961 acagcgctct ctcaccctcg ccggattcta tcagtatact ctggtggttg ggagtgtggc
    10021 cgggcgtgag tacgcaccgg acgtggggcg tgatctgcgt gtcgccctcg atcagttcca
    10081 gacgacctgt ctcccagacc ggcagtagat tctcggccag gtaagaagca cgagtgcgtt
    10141 cgttcggatg ggtggcatcc tcccactcgc ccctctgtgc gatgtaggtg gcgttgggga
    10201 atgtcgggat tgctctgccg gactgcagga gcgtgttgcc accggcgtgg tcgaagtgca
    10261 ggtgggtatt gatcacgatg tccacgtctg ccggtttgac gccctgttcc gcgagcagca
    10321 cgggcaattc gccggcgctc tcgatcccca taatggacat cgttcgcggg cgcagcttgc
    10381 tgcctatgcc cgtgtcgatg actatcgtgt gctccccatc ccgaatcagc aggcagttgg
    10441 ccacgattgc cacccggttg agttcgtccg gggcgtgctt ggcctcccac atctgccttg
    10501 gaacgacgcc gaacatgccg ccgccgtcca cccgcatgag accgtccgag atgagggcca
    10561 gatctatgtc gccgagccgg acacttcttg tctttgctga ggctgtcgtc acgcgatttg
    10621 gtacctgttg agttcctccg ccagctcggc ctgagggaag acggccttgg cctgctgcag
    10681 catctcctgc cgatcgtaga acggccagat gtgggtcagc agcagttgtc tggcaccagc
    10741 cgaagctgcc acttcggcgg ccatcgccgg tgtcagatgg atggcgtgat cgggatcgaa
    10801 gtcctcgggc ccgatggatg cctcgcacag cagcagatcg caccctcggg cgaagtccgc
    10861 taactcgggg agtggacccg tatcggcgga gaagaccatg gagccgcggc catcctcgat
    10921 gcgaagggcg taggcaggga ccgggtgtac cgtcttgaga aaggagatcg acagtgcgtc
    10981 gacggacagg gggccggccg gatcgtactc ccgcaccgag aagacctgct cgcacagctt
    11041 gcgagtgtcc accctcagca cctctgcaag tcgggcgaat cgttccacct cgccgggtgg
    11101 catgaatacc ggcagggttg gatagacctt tcccttgccg tacagccgag cgtagaagag
    11161 gggaaagata tccagcaggt ggtcggcgtg aaggtgactg aagagcagcg cggtgacgtt
    11221 gtccatctcg ccggcctctt gcagcttgct caccaccccg ttgccgcagt caaccagcaa
    11281 tccggtttgc ccgtcccgaa tcaggtatcc cgacgatgcc tggtggggcc ccgggtaggc
    11341 ggcaccggat cccatcacta ccagttccat ggcggcgagc cctccctctc tcatgctctt
    11401 ttggcatagt gagggaaata gtagcacaat ccctacctga gatggcttcg cggagctgta
    11461 ggaacaaggt ggcgtcaagt tgacgcgctg atctgcaaat ggcggcagcc cttcaattac
    11521 ctacctagta cgctcccttg cccctgagga ccacccagat cgtcttccag aggatcagca
    11581 gatccagaac cggggagtag ttctggatgt agtagaggtc gtactccgtg ttgaggaaca
    11641 tcggacggtc gctgcggccg ttcacctgcc accatccggt catgccgggc atgacggaga
    11701 gccgcttgcg ctgccacggc tggtagcgct ccacgatcca gggttgctcg gggcgcggcc
    11761 ccaccaggct catatcgccc ttcaggacgt tgaacagctg cgggaactcg tccgcgctca
    11821 tcctgcgcag tatcttccct acgcgggtga cccgcgggtc ttccttgacc ttgtagaccc
    11881 cgtcggcgta gacgccgtcg ctctccagct tggccaccat cttatcggcg ccattcacca
    11941 tcgtccggaa cttgtacatc cagaacagcc ggccgttctc tccgacccgc cgctgcttga
    12001 acagcgcagg acccttggag tctgtcttga tagccagggc gatggcgagc atcacgggcc
    12061 ccaggatgag caggagtgcc gacgagagaa ccacgtcgaa cgcgcgcttg aaggcccggt
    12121 cgaagccggt aatgacaggg tccctgagac cgatcagcgg aatcccccag aaatcttcta
    12181 cttgggctcg cacggagacc atctcgaaca ggtccggcac cacccgcacc ctcaccgggt
    12241 acgactggag actgaggatg atctgggcga tcttgtcgtg ctcctcgcct cccagggcaa
    12301 cgatgacatc gtggacactg ttctgctcga tcacgtgggt aagatcgttg gtggtgccga
    12361 gaaccggccg cccctcgatc tccttaccct gtagctcggg gtcatcgtcc acgaagccga
    12421 cgacgctcag tccggaccag ggttgggagc cgatgatgta ggccagctct ttgccgacct
    12481 ttgtggatcc caccagcaca acccttcgga cgtagtagcc tccggcgacc atcgacttta
    12541 ccacatacct ggtggccagc cgatagttga ggagcagggc cagatcgatg gctccgaagt
    12601 aggtgaagag catcctggag cggaactcga cgttgaacaa gtagaagaag gacgccaggg
    12661 tgaggagacc agccgccgta gccagcgcca cggcacgccc ctcgtcgacg atactggata
    12721 gacagcgcgg gtcgtaggcg ccgaacagcc tcaggaagaa gctccagaag atggcgacag
    12781 ccaggagaat atacagatcc agaaagctca tcccgatgct ttctcgaccg aagggcatta
    12841 cccctcgcgt gatctcagcc aggaagagcg ccagctctgt aaagaagatg tccgctacaa
    12901 agaggaatat gatgaagcgg ttgctgtatt ccttcaaagg ggcaacctcc tcgcggggaa
    12961 ctatcctaca agatcgacat agagctgtcc agtcctcttg accatcgcct cctgcgagaa
    13021 gcggcggagg atggtttcgt ggccggcagt gcccatcgct tgtctctcgg cgggagctcg
    13081 cagtagcctt gctatcgctt cagcagctgc cgcgggctca ccgaccggtg tgagcagccc
    13141 ggttacgcct tcctggacga tgtctcgcgt gcctaccgcg gccgtggcta caaccggttt
    13201 gcccaatgcc atggcgtcca gcaccgtgta gggcaaccct tcccagaggg aagtcaggac
    13261 gaacaggtcc agcgtgctca tgatgtccag cacgtcggtc cggtacccca ggaagtgcag
    13321 gcggtcggag actcccaact cgcctgccag ggactcgacc tgctccctca gttcgccgtc
    13381 gccgcaccag aggacgtgaa ccccgggtac caactgcacc aggtggctgg tcattcggac
    13441 gaagtcaaag gggcccttct gaggtgtgaa acgagcgacc gtacccacca ggtactcggc
    13501 ctcgggtgcc agggtctccc ggagtcgggc gacgatacca tcgctaggaa cggcgggcac
    13561 gcggatgccg ttctcgatca gcgctatcct atcggacttg atgatcccgt tatccacggc
    13621 ctcggccatc tcggttggtg agagggcaat tatccggctg gtcgttaacc ccgccatccg
    13681 ttccagctgc aggtagaaac tccgtttgcg gcccggcttc tggttgagga agtagaagcc
    13741 gtggggggtg tggactgtaa ccttgacccc tgccgcccgc gccgcgaaac ggcccaggaa
    13801 gccggccttg gagctgtggg tgtgaaccag gtcgtagcgc ccccgtgcca ttagcgccac
    13861 caactgtgag aatgagcgca gatctgagcc cggcgaaatg gcgcgctgca tcggcacgaa
    13921 gtgctgcttt atgccggcct cccgcacctc cttcgtgaag gtgacgtcgt cgaatgcctc
    13981 agatcgtatc tccggcgcgg ccacctccac ctcgaactgg ttgcgatcca gccctcggat
    14041 cagcgccagg agatggcgct tggtcccgcc tattgtggct tccatcacct ccaggaccct
    14101 tatgggtctg gagttgattg atttacccaa ttgctctcac caacatggag aagatgagag
    14161 ggaagggggg gggggggggg gggggggg
//
LOCUS       DATALS010000292         4387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_329152, whole genome shotgun sequence.
ACCESSION   DATALS010000292 DATALS010000000
VERSION     DATALS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329152"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..715)
                     /locus_tag="VHS06_06515"
     CDS             complement(26..715)
                     /locus_tag="VHS06_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX2987808.1"
                     /translation="MAAVGGNESSIDGRGTSPHEANILAVEGTTPAETDALCLELAAA
                     RQEIERLRSDLLSTVSHELRTPLTLIRTSAGLLLDTEPDPAMRERLLRNIKQSADRMQ
                     ALVTDMLDLVRLRNGRAELAIELLDVGELVESAASLMKPLLDEKEQRLELDMPLVAPR
                     VMGDQRRLEQVLLNLLSNANKFAPQVATVRISVSEDAESVMLSVADSGPGTIVTYSQY
                     TSEKVAAVPAY"
     gene            948..1415
                     /locus_tag="VHS06_06520"
     CDS             948..1415
                     /locus_tag="VHS06_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX2987809.1"
                     /translation="MSTVESVQLTREGWEQLHKELVLCKKARDSRLAGLMNVAREAEP
                     GGAVVRHEQQEIDSADRRIAYLEDILSRAIPISAADRTLGVIGVGSRVTVSWEGGEEE
                     SYLIVGPPEVDLATNRISYESPVGRALMERSEGEWVEVKTPGGMSRLEIAKVI"
     gene            1789..2259
                     /locus_tag="VHS06_06525"
     CDS             1789..2259
                     /locus_tag="VHS06_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX2987810.1"
                     /translation="MRALEKPLPFTREGTKELELEIALLEHCRKQIDDECRAAPDNVR
                     ESLQRELAAVDDQVSRLREVLIHGEIVQNTGLTVAVGSEVTVEAEYGKPTFTIVGPVA
                     ATPRRGAISYESPLGKALLGAALGDWVEVPWEDGHRRLRIVAISTPALENQLHR"
     gene            complement(2444..3439)
                     /locus_tag="VHS06_06530"
     CDS             complement(2444..3439)
                     /locus_tag="VHS06_06530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2987811.1"
                     /translation="MVQEETYLLMREHVAERYRMNWTKSLKDAYALDPDFLTVDPASI
                     DFLVSNRETLLELFADPATFDDLVDLFVELSLEFSYNSNQFIRVSNSNRDRMVEIYRS
                     YLRGMERLVAQERSTESLSRSMEALVRDHFVELRRYLDDFSAEGLRSGSQDNVFLASV
                     VCREYSPELQLSILGIDPSELLEPILDIGCGKSGQLVRFLRGLGLAAFGADRVVEASD
                     YLMKADWLDLPMEPGSWGTVISHMGFSNHFIFQHLYRYGSPEDYARQYAAILASLKVG
                     GSFWYTPGLSFVESYLPPQQFSVVRRRVSLPSAIGDTPAPSAAGNLPYSTQVTKK"
     gene            complement(3557..>4387)
                     /locus_tag="VHS06_06535"
     CDS             complement(3557..>4387)
                     /locus_tag="VHS06_06535"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX2987812.1"
                     /translation="DREGVKKHIDFVVNAGVDGIFILGSIGEGPFVRPHVAAAMAEAT
                     VEAVAGRVPVIAGVIEPSTARTIDAVKNLSGRGLAGYVAAAPFYYGGYVNRELDKHFR
                     KIADAADLPVLLYNIPVNTKTPMNANLILGLQNHPNIAGIKDSSGDWPVIQPVLLNKE
                     KDFVFFQGNQYMCAVSLMMGADGLVPGHANVWPKLVVDLMKAGKERDFKAAFALQNTL
                     DKLVTLRGRAPAYTYKIVTKALGLTDEYVSTPLPMLTPEETDAFIKTSIELGVPIPRE
                     "
BASE COUNT          912 a         1311 c         1345 g          819 t
ORIGIN      
        1 cgagagttgt acggccgtga tgcactcaat atgcagggac tgctgccacc ttctcgctgg
       61 tatactgtga gtatgtgaca attgtgccgg ggcccgagtc cgccacgctc agcatcacgc
      121 tttccgcatc ctccgagaca gagatgcgga cggtcgcgac ctggggggcg aacttgttgg
      181 cattggagag gaggttcagc agcacttgct ccagacggcg ctggtcgccc atcaccctgg
      241 gcgcgaccaa cggcatgtcc aactccagac gctgctcctt ctcgtcgagt agcggcttca
      301 tcagcgaggc cgcactctca accaactcac ctacgtcgag caactcgatg gccaactcgg
      361 ccctgccgtt gcgtagcctg accaggtcga gcatgtcggt gaccagcgcc tgcatccgat
      421 ccgcgctctg ctttatgtta cgcagcagcc gctcacgcat ggcgggatct ggttcggtgt
      481 ccagcagcaa acctgcagag gtacggatta gcgtgagggg agtgcgcaac tcgtgcgata
      541 ccgtcgacaa caggtcgctg cgcagacgct ctatctcctg tctagccgcg gctaactcca
      601 ggcaaagagc gtccgtctct gcgggcgtcg ttccctcgac ggcgaggata ttcgcctcat
      661 gtggagacgt gccacgccca tctatcgacg attcgtttcc accgaccgca gccatgtaca
      721 cctctttcca ctaggccaac gacacaattg tcacatactc acagtatacc agcgagaagg
      781 tggcagcagt ccctgcatat tgagtgcatc acggccgtac aactctcgcc gacgccggcg
      841 ccgtaatccc ctcttccggc aaccttatcg cccttttcac gttccgttca tgtggtttcg
      901 gcggggtttt cacggacaaa agataggatc ttcccaggag gagaacgatg agtaccgtcg
      961 aaagcgtcca actgacaaga gaaggctggg agcaacttca caaggagttg gtgctctgca
     1021 agaaggcacg cgactcgcgt ctggccggcc tcatgaatgt tgccagggag gcagaacctg
     1081 gaggcgcggt cgttcgccac gagcagcagg agatcgactc cgccgatcgg aggatagcat
     1141 atctggagga cattctttcc cgggcgatac ccattagcgc tgccgacagg acactgggcg
     1201 tcattggcgt cggatcccgg gtcacggttt cttgggaagg cggcgaagag gaaagctacc
     1261 tgatcgtcgg cccgccggag gtcgatctcg cgacgaaccg gatatcttac gagtcgcccg
     1321 tcggccgtgc cctgatggaa aggagtgaag gggagtgggt cgaggtgaag acgccgggcg
     1381 gaatgtcgcg gctggagata gcaaaagtga tatgacaact ccgcattcgt cgccgcggag
     1441 aatcgccgca cgggatcaga acggacaaag gggggtggcc cacgacaacc gaaccgaaca
     1501 tcgttgcctc aactgcggtc tggcaatctc ctggccgtcg atcatcgtga acgggatgga
     1561 gtactgctgc ggcggatgcg cccttggggg cccttgctac tgctcctacg acgcggcggg
     1621 ttcggaggaa ccgatcgaag acccggtaaa cagggtagat gggccaagga gatcgataca
     1681 gtgggaaaga tagctgctgg cggccggccg gattcgcgcc gaagtgatca cgcacagcgg
     1741 agagtggtca cgaggcaaga tgagctgcca gaaggagagc tgagcgacat gcgcgcatta
     1801 gagaagcctt tgccgttcac tcgagaagga acgaaggaac tggaacttga gatcgccctg
     1861 ctggagcact gccgaaagca gatcgacgac gagtgccggg ctgccccaga taatgtccga
     1921 gagtcgctgc agagggaact ggccgccgtg gacgaccagg tttcccggct gcgcgaggtg
     1981 ctcatccatg gagagatcgt ccagaacacc ggcctcacgg tagccgtggg cagcgaggtg
     2041 acagtagagg ctgagtatgg aaagcccacc ttcaccattg tcggtccagt tgcagccacc
     2101 cccaggagag gcgccatctc ctacgagtcc ccactgggca aagcgctgct aggggctgcg
     2161 ctgggcgact gggtggaggt tccgtgggag gatggccatc gtcggctcag gatcgtggcc
     2221 atttccacgc cggcattgga gaatcagctg cataggtaga aactggatca cgctgacgtg
     2281 ggagcagggg gatctgaccc tcgttctgac gaacccctac gcccccccgg gcacccacgc
     2341 tacacgccgg acgagttaag gaaggtcgtg gagtcgatca ggtagtggac cccatcaggc
     2401 aaggagtgca ggcgagcttt gtgtctgcac tccctgccgg gcgctacttc ttggtaactt
     2461 gggtcgagta agggaggttg ccggccgccg agggtgctgg agtatcccct attgctgagg
     2521 gaagactgac tcttcgccga acgacggaga actgctgagg gggcaagtac gactcgacga
     2581 aggagagtcc aggagtgtac cagaaggatc cgcccacctt gagcgacgcc aggatcgccg
     2641 cgtactgacg ggcgtaatcc tcgggtgaac cgtacctata cagatgctgg aagatgaagt
     2701 ggttggagaa gcccatgtga gagatcaccg tcccccaact ccccggctcc atcggcaagt
     2761 ccagccaatc cgctttcatc agatagtcgg aggcctcgac caccctgtcg gcgccgaatg
     2821 cggcaaggcc aagtcccctc aagaaccgaa ccaactgccc cgacttgccg cacccgatgt
     2881 ccaagatcgg ctccagcagt tccgaaggat cgatgcccag gatagagagc tgtagctccg
     2941 gcgagtactc ccggcaaaca accgacgcga ggaagacgtt gtcctgcgaa ccggatcgga
     3001 gaccctcagc cgaaaagtcg tccaggtagc ggcgcagttc cacgaaatga tccctgacca
     3061 gcgcctccat gctccgagag agagactcgg tcgagcgttc ctgggcaacc agccgttcca
     3121 ttcctcgcag gtacgatcgg tagatctcca ccatacgatc acggttagag ttgctgaccc
     3181 tgatgaactg gttgctgtta tagctgaact ccagagacag ttccacgaag agatcgacca
     3241 gatcgtcgaa ggtagcaggg tcggcgaaga gctccagcag cgtctcgcgg ttggacacta
     3301 agaagtcgat gctggccgga tccaccgtta agaaatcggg gtccagcgcg taggcgtcct
     3361 tgagggactt ggtccagttc atcctgtagc gctccgcgac gtgctctcgc atgagcaggt
     3421 aggtctcttc ctgcaccacc tcgctgacct cccgtgaatt cgtcccatca cagccggagc
     3481 caggctctac gcaagcctgg ctccaaagca ttccacgttg gcgataatgc cccgtggtcg
     3541 tctcacctca aggtggctat tcccgcggga tcggcacgcc cagctcgata ctggtcttga
     3601 tgaacgcgtc ggtttcttcg ggggtcagca tcggcagcgg cgtgctgacg tattcgtccg
     3661 tcagtcccag agccttggtc acgatcttgt aggtgtaggc cggtgcccta ccacgcagcg
     3721 tcaccaactt gtccagggtg ttctggaggg caaacgccgc cttgaagtcc ctctccttgc
     3781 ccgccttcat caggtccaca accagctttg gccaaacgtt ggcatggccc ggcaccaacc
     3841 catcggcacc catcattagg ctgacggcgc acatgtactg attgccctgg aagaagacga
     3901 aatccttctc cttgttgagc aacaccggct gaatcaccgg ccaatcgcct gaggaatcct
     3961 tgatgcccgc gatgttggga tggttctgga ggcccagtat caggttcgcg ttcatcgggg
     4021 tcttggtatt gacggggatg ttgtacagca gcaccggaag atctgccgcg tcggcaatct
     4081 tgcggaagtg cttgtccagc tcgcggttga cgtagcctcc atagtagaag ggcgccgcgg
     4141 caacgtagcc cgccagcccc ctgccggaga ggttcttcac ggcgtcgatc gtccgcgcgg
     4201 tgctcggctc gatgacaccg gcgatcaccg gcacccgacc ggctaccgcc tcgaccgtag
     4261 cctcggccat ggccgcggca acgtgaggtc ggacgaaggg gccctcaccg atgctgccca
     4321 ggataaagat gccgtcgacg ccggcattca cgacgaagtc gatgtgcttt ttcacgccct
     4381 cgcggtc
//
LOCUS       DATALS010000293         3827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_254248, whole genome shotgun sequence.
ACCESSION   DATALS010000293 DATALS010000000
VERSION     DATALS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254248"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VHS06_06540"
     CDS             <1..242
                     /locus_tag="VHS06_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEX2987813.1"
                     /translation="NCLEPGVIRTKLLRAGWGGSFGGDPEAGASKVVYLATSADVEGV
                     TGKYFRYNQFVAPSPLAEDPEVRRRLWDLSVQLTS"
     gene            287..922
                     /locus_tag="VHS06_06545"
     CDS             287..922
                     /locus_tag="VHS06_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEX2987814.1"
                     /translation="MSVEETLRRRARAHLLAGSDVRRLVADQCADQILEAARVVADCF
                     RAGGKLLICGNGGSAADSQHMAAEFVSRLSADFERPALPAIALTTDSSFLTAYANDYG
                     FEGIFSRQVEALGKAGDVLVGISTSGNSANVIRAMEAASSQGMRTIALVGNGGRMAGL
                     ADVALVVPSRNTQQVQETHLALEHLVCDLAERFLYGVSGQKKGTLPRRIAS"
     gene            complement(868..2181)
                     /locus_tag="VHS06_06550"
     CDS             complement(868..2181)
                     /locus_tag="VHS06_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015233951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HEX2987815.1"
                     /translation="MNRPELIEEARQRAIDILRGNSRDIGLLSGRHAYQQVNGRDGMI
                     CGLGLMLQEDRDLWDVHRHSIESLAAGQSRLGNIPFGVGFPDVPDESFIATGGTFAKV
                     RTDDDRRRPVVDTIHAGCVDNCLWYILGHYMYQTSTSDTTFLGRYWSSIERAYLWLQY
                     QDVNECGLLEVHEAMDWADLFANRYNVLYDNALWCAAHRAMGYLAAAIGKDPAPYFAE
                     AESTTLKLNLLLWVGPEVDRNLEWVQENRTEWLYPIKRTDTELVVRPYYLPYMGFRDF
                     ADRFDTLGNLLAILLGVASPEQSARILDYAHGVGLDEPYPIQVLYPVIRPGEPDWRHY
                     YLVRNLNQPHHYHNGGIWPFVGGFYVAALVKAGRVEEAERQLARLAELNSLGRNEPWE
                     FNEWFHGVSGRPMGFDGQSWSAAIYLYAYEAIRRGNVPFFWPDTP"
     gene            complement(2185..3090)
                     /locus_tag="VHS06_06555"
     CDS             complement(2185..3090)
                     /locus_tag="VHS06_06555"
                     /inference="COORDINATES: protein motif:HMM:NF036773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HEX2987816.1"
                     /translation="MWGRGASTFRTQEVSLPVRLPKELAAGRRERARTIVACPITFRV
                     RLAAGVDRVEFHTEVDNRARDHRLRVLFASPDSPQKVRAEGHFALLRRPAQPVWNGRW
                     REPPARTHHTLGLVAAGDLAVVSRGLPEYEAVPKVGGGVDVALTLLRCVGWLSWPDLS
                     TRPGAEEPHVPTPDAQCLGTHSFQYALTLRGDDDAATLLRASQDYRVELVRGPEGIDL
                     ERTLAVEGSSFAFSALKGAEDGDGVVVRLFNPGDGPAIAQVTGAEVAVHRCRLDEEET
                     QTQQVRAIAMKAGEIATARIIPGGD"
     gene            complement(3054..3284)
                     /locus_tag="VHS06_06560"
                     /pseudo
     CDS             complement(3054..3284)
                     /locus_tag="VHS06_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
BASE COUNT          671 a         1214 c         1216 g          726 t
ORIGIN      
        1 ccaactgcct ggagccgggc gtgatcagga ccaagctctt gcgggccgga tggggaggct
       61 catttggagg ggaccccgag gcgggggcga gcaaggtggt ctacctcgcc acctcggccg
      121 atgtcgaggg agtgaccggc aagtacttcc gttacaatca gtttgtggca ccatcacccc
      181 ttgccgagga ccccgaggtc aggcggaggt tgtgggatct gagcgttcag ctaacgtcct
      241 aggcacgcgg cgtgatattg atctcgctcc tggaggtcgg ccgatcatgt ccgtcgagga
      301 aacgctgcgt cggagggccc gcgcccatct gctggcgggc tcggatgtcc ggagactggt
      361 cgcggaccag tgcgccgacc agatccttga ggctgccagg gttgtggcag actgttttcg
      421 cgcaggcggg aagttgctga tctgtggcaa cggcgggagt gccgccgact ctcagcatat
      481 ggcggcggag tttgtcagcc ggttgagcgc cgacttcgag cggcccgcgc taccggccat
      541 agcgctgacc accgacagct cctttcttac tgcgtacgcc aacgattacg gcttcgaggg
      601 gatcttctcc cgccaggtgg aggcgctagg gaaggccggc gatgtcctgg tggggatcag
      661 caccagcggc aactcggcca acgtgattcg cgcgatggag gcggcctcgt ctcagggaat
      721 gcgcacgatc gcgctggtcg ggaacggcgg ccgtatggcg ggtctggccg acgtcgcgct
      781 ggtagtgccc agccgcaaca ctcaacaggt acaggagact cacctggcgc tggagcacct
      841 ggtgtgcgac ctcgccgagc gattcctcta cggggtgtcc ggccagaaaa agggtacgtt
      901 gccgcgccgg attgcctcgt aggcgtagag gtagatcgcc gcagaccagg actggccatc
      961 gaaccccatg ggccggccgc taacgccgtg gaaccactcg ttgaactccc agggctcatt
     1021 ccgtcctaag ctgttcaact ctgccagtct agccagttgg cgttccgcct cctccacccg
     1081 accggccttt accagcgctg ccacgtagaa ccctcccacg aagggccaga tcccgccgtt
     1141 atggtagtga tggggctgat tcaggttgcg caccaggtag tagtggcgcc aatcgggctc
     1201 ccctgggcgg atcacagggt agagcacctg gatcgggtag ggctcgtcga gccccacccc
     1261 gtgggcgtag tccaggatcc tcgcggactg ctccgggctt gccaccccaa ggagtatggc
     1321 cagcaggttc cccagggtgt cgaacctgtc ggcgaagtcg cggaagccca tgtagggcag
     1381 gtagtaggga cggaccacca gttccgtgtc ggtccgcttg atggggtaca gccactccgt
     1441 gcggttctcc tgcacccact cgagattgcg gtcaacctcg gggccgaccc acagcaggag
     1501 gttcagcttg agggtagtgg actcggcctc ggcgaaatat ggggcgggat ctttgccaat
     1561 agccgcggcc aggtagccca tggcgcgatg tgcagcgcac cacagggcgt tgtcgtagag
     1621 gacgttgtag cggttggcga agagatcggc ccagtccatc gcctcgtgga cctccagcaa
     1681 gccgcactcg ttcacgtcct ggtactgcag ccagaggtag gctcgttcta tgctggacca
     1741 gtaccggccg aggaaggtcg tgtcgctcgt gctggtctgg tacatgtagt ggcccaggat
     1801 gtaccagagg cagttgtcga cgcagcccgc gtgaatggtg tccaccaccg gccggcggcg
     1861 atcatcgtcg gttcggacct tggcgaacgt gccccctgtg gctatgaagg actcgtccgg
     1921 cacgtccggg aacccgacgc cgaaggggat gttacccagc ctgctctgcc cggccgccag
     1981 cgattcgatg gagtgacggt gtacatccca caggtcgcga tcctcctgca gcatcaatcc
     2041 aagtccgcag atcatgccgt ccctgccgtt gacctgctgg taggcatgac gaccgctgag
     2101 caatcctatg tcgcgggagt ttcccctcaa gatgtcgatg gccctttgcc tggcctcctc
     2161 gatcaactcc ggacggttca tcgatcagtc cccccctggg atgatgcgtg cggtggcgat
     2221 ctctcctgct ttcatggcga tggcccggac ctgttgagtc tgcgtttcct cttcgtccag
     2281 gcggcatcgg tggacggcaa cctccgcccc tgtgacctgt gctatggccg gcccatcgcc
     2341 tggattgaag agccgtacca cgacgccgtc tccgtcctct gcccctttga gcgcggagaa
     2401 ggcaaaggag gagccctcga cggccagggt tcgctccagg tcgatcccct ctggaccccg
     2461 gaccaactcc actcggtagt cctgagatgc cctcagcagc gttgcggcgt catcgtctcc
     2521 ccgtagagta agggcgtact ggaagctgtg tgtcccgaga cattgggcat cgggagtcgg
     2581 gacgtggggc tcctcggcgc ccggcctggt ggagagatcc ggccagctga gccatcccac
     2641 gcagcgcagc agcgtgagcg ccacgtccac cccaccgccc actttcggca cagcctcgta
     2701 ctcggggagc ccgcgggaga cgaccgcgag gtctcccgcg gcgacgagtc cgagggtgtg
     2761 gtgggttcgg gcgggtggtt ccctccaacg cccgttccag acgggttggg ccggccgccg
     2821 cagcagcgcg aagtgcccct cggccctcac cttctggggc gagtccggcg aggcgaagag
     2881 cacccgcagt cggtggtcgc gagcgcggtt gtccacctcc gtgtggaact cgacgcgatc
     2941 gactcccgcc gccagccgca cccgaaaggt gatcgggcag gccacgatgg tgcgagccct
     3001 ctccctcctg cctgccgcca gttccttcgg gagtctgacg ggcagcgaga cttcctgggt
     3061 acgaaaggta cttgcgcctc ggccccacat tgcgcgcaga tcaccgtgtg catctcacga
     3121 ggatcgcgcc ccgaacgctc gcgacgacgg gctgctcggc aagcagggca cctctgcggc
     3181 tcattcatga gtcccttttc tgcaaagaac tcctgttcgc cgacagtgaa cacgaattcc
     3241 tgcccgcagt ctcggcattg cagggtcttg tccgtaaagc tcacttttcc ctgaccccct
     3301 tcgcttgtgg agaagtctgg tcacagttta gtcacttgac ggaaccgcat gcgggatgcc
     3361 tttgccagaa aaccaggacg aaggtccgct gggtcgcgat tgcctaaacg tgccggggct
     3421 acaatagcac gtcgaatttg agcgtgcaac cattccagaa ggcccaaacg gccaaaccac
     3481 tgtacattat gaacattatc cgaccacacg atcgacatag tgcacacaga taagctaaga
     3541 ggccacattc ggcctctatt cgggtctatt cgcacttctg cctggcaata gctgtctact
     3601 agaagttcct cgcattcgta tgagcattag agcggccgca gcagcggttt gacaccttac
     3661 gccgccgaaa tgtaagctag gccttacgga tgccgatagg gccagcaggg aggagccgtt
     3721 gcagcactac aagaccgtta tgagccgtgg tcttcgggtc gtgaccactc ctatgcccta
     3781 cacccgatcg gccagtatct ccctcttcgt cggcacgggc tcgcgtt
//
LOCUS       DATALS010000294        10193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_284381, whole genome shotgun sequence.
ACCESSION   DATALS010000294 DATALS010000000
VERSION     DATALS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284381"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="VHS06_06565"
     CDS             <1..1203
                     /locus_tag="VHS06_06565"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX2987817.1"
                     /translation="ILQIEVPAERVERLKKLAQVDIPGISDEVFTFLIKQQVQFANIQ
                     YARFLAGAKFSEQDVDDVLSNSIDIHIHGGSDPMERLMLEDDIAMDYTDIGMRAMVVK
                     TWYSPSASRNALVQKAVNRYAEERGLRPIQCFGGVTLAYSMGGINPEAVKKCLGFPGM
                     KYVWMPMVDSYHHRRVVYDDWSGFGLHFLDENRKVVPAMQEILRIVADNDLILGTGHY
                     PYEDSAILFEEARRLGVTRMEVVHPAHIHSKHTIENMKAQVARGAKIMLSGLGAMAFP
                     LHESGPLYAARVVKEVGPENVVYGSDFNQLQNFPPSMAARWMVKLLLAYGCTKEELYQ
                     VFQVTPAKHLGIELPPPLGTPAHPGWPRDDSAKAYVGHPQADPTVRMRSHGLYKPAEW
                     TIPSNGES"
     gene            1253..1453
                     /locus_tag="VHS06_06570"
     CDS             1253..1453
                     /locus_tag="VHS06_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987818.1"
                     /translation="MKRLVTFLLAITTATVLLAGCSQAQPAATPAPTKAPASAAEPTK
                     AAAPTVQPTIAAQPTAAPRQEG"
     gene            1512..2342
                     /locus_tag="VHS06_06575"
     CDS             1512..2342
                     /locus_tag="VHS06_06575"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX2987819.1"
                     /translation="MDVGTRLLTPYLEKELGVPIQVVNKAGASGQIGEMEMLKSKPDG
                     YTICATLVPGTITVYLDPERKATYGRKDFQPLALHVSEPAILVVKGDSPYKTAKEFIE
                     AAKANPEKVKISSSGIMSPAHMATLQFQKVTGSKFAIAQFDGAAPALTALLGGHVDAN
                     IGWGGNFVGPAKNGDIRALAVMESQRSEFFPNVPTMEELGYKVSFSSARGFSVPAGTP
                     KDVVDVLDAAFKKAINNDEHKKKIQEASLPLKYMDAAAYGTYWDETETIIKPMMANPQ
                     "
     gene            2497..3198
                     /locus_tag="VHS06_06580"
     CDS             2497..3198
                     /locus_tag="VHS06_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX2987820.1"
                     /translation="MGRIWDDVLTEKDKLVYEAAGYGQRAGLGRKPAIIIIDVNYNFV
                     GDKPEPILESIKRFHMSCGEAGWDAVRGLQQILPLARERKVPVFYSTGKPRSGAQRIF
                     AGQWAAKNHRTGEAGEGDGNQIVAEIAPGPDDILIPKEKPSLFFATPLLSYLIYHNVD
                     TVFVCGTTTSGCVRATVIDAFSYNFKVGVIEECTFDRGEVPHKINLFDMNSKYADVMR
                     VTEVMDYLRSLPADR"
     gene            3323..4315
                     /locus_tag="VHS06_06585"
     CDS             3323..4315
                     /locus_tag="VHS06_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2987821.1"
                     /translation="MSKVRITLACGDYDRTRALLDGRVVPDGIDLNYLPLEPEETFWR
                     MIRHQEFDVSEMSLAMYAIECARSTSPFVAIPVFPSRMFRHSSLYVRQDSSIERPEDL
                     IGKRVGVPEYQITAAVWVRGILKDQYGVSPEQIEWVTGGQEHPDRKEKIPLQPPPGVH
                     VEQAPPGRSLNDMLLDGEIAALLAARTPSIFRTRSSQIRRLFRDCRQVEKEYFNQTGI
                     FPIMHTIVVRRDVHQQYPWVASSILKAFIQAKRLCYQMISSPTSLRYLLPWMVADVEE
                     MVNLMGEDPWPDGLSPNQKTLQTLMGYLWDQGLTPRRLDPEELFAPNTLESFKI"
     gene            4488..5570
                     /locus_tag="VHS06_06590"
     CDS             4488..5570
                     /locus_tag="VHS06_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2987822.1"
                     /translation="MSTIKDVAERTGLSVCTVSHAIHGTRHVKPETRERVLAVAEQIG
                     YRPNRLARGLKTKRTNTVGCLVSHVRVPSVGVLVTGAEEVLVAHGYSPILSCTHLQSS
                     FELNAIDTLTDLRVDGLLATGCSEMFVNKMAGFTEFPVVLVHCHPASATVDCVDIDDE
                     LAMRRATEHLLSLGHRRIGFVGRASPRAMFAARRTGYSLALLSHGIPFRDELICEVQP
                     EPREVWQGMSRLLSAAPRPTALVVAHAQLAFEVVRFLREKGVRVPDDIAVVGMGSDPW
                     NDLVDPPLTRISVPSLDLGRAAADLLVSRLEGRGPDRPQSVLLDAPLIIGGSCGSPMA
                     STPGRVAGFNQDGSGVFSSSVENSVE"
     gene            5665..6153
                     /locus_tag="VHS06_06595"
     CDS             5665..6153
                     /locus_tag="VHS06_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2987823.1"
                     /translation="MDEQEFLDSREEMEQILREEVVGYLGLSDNGKPYVVPLNYAYMS
                     GKIVFHCALTGQKLDAIRRNPHVCFTVGRQVGDVRDHANDPVCHPDSDSVICYGMARL
                     LEDHTEREAALNAFNRRFRPSEPDLPMKRIERCMAVEITITKMTGRRERARHRAYWRS
                     TF"
     gene            complement(6625..8544)
                     /locus_tag="VHS06_06600"
     CDS             complement(6625..8544)
                     /locus_tag="VHS06_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987824.1"
                     /translation="MILDYWGQHRGVHVNPPRLDACLKNRKGYTDDKGVIQERAVECG
                     REFGIGNLSAVKPYKDKNTLISDLLERIPAKLQVQEFHHVLALGVSDAPNGDDTFYIS
                     DPAVKSTKPDSIDRYVPLSMVLYDRYNNDYIQAMSAYDNPAASKGSFALRLFSPAELL
                     VRDAQGRRSGIDPRTGAKYDEMPDCVYYEDGIDDHSSQPLAIGSTKTFYCYGPQSGRY
                     DIQVIGIGTGSYRVEVLAYDDTGQPSTGELSGQAQQGAVDSYRAEYSSVPGVPVRVGP
                     TNEPPTLTAPASLQVEYSDNMLFMVSATDPDDSSSDLRFSAKGLPADLTLTDNLNGTA
                     SVSGTVTASAWTYPVDLTVTDPHGLTDTKQVSVVVAKEKAATEYTGSLFALTSSMTSL
                     TTQLQLAARLTEEADGAAGDPTKAKVTFELFKSSNLSTTPDQVISGVPVNADGTAQTM
                     VSLGVDSWVVNVKISSDNGYWTTNSVGSSVVMVALPDPSRRVVGGGWIPYDGSLNGKA
                     NFGFTVNYQKTGNPKGQSVFVYGSKGDGFNYVIKSNSWQGGGLGFASDPSKATFSGKC
                     TVQKIDRVTGSVAPSWGNYSYTVGLTDGDQANPKGPDKFAITIMDANNNVWKQVGTAN
                     LPVQLGGGQVTVQSK"
BASE COUNT         2191 a         3012 c         2819 g         2171 t
ORIGIN      
        1 atactgcaga tcgaggtccc cgccgagaga gtcgagcggc tgaagaaact ggcgcaggtg
       61 gacatacccg gtattagcga cgaggtcttc acctttctga tcaagcagca ggttcagttc
      121 gccaatatcc agtacgctcg ctttctagct ggcgccaagt tctccgaaca ggatgtcgac
      181 gacgtactct ccaactccat cgacatccac atccacggcg gctccgaccc gatggagcgg
      241 ctaatgctgg aagacgacat cgccatggac tacaccgaca ttggcatgcg agccatggtg
      301 gtgaagacct ggtacagccc atccgcatcc cgcaacgctc tggtacagaa ggctgtgaac
      361 cgctatgccg aggaacgagg gctacgcccg atccaatgct tcggaggcgt caccctcgcc
      421 tactctatgg gcgggatcaa tccagaagcc gtgaagaagt gcctgggctt tcccggcatg
      481 aagtacgtct ggatgccgat ggtggactcc taccatcaca gacgggtagt ctacgacgat
      541 tggtccgggt tcgggctgca tttcctggac gagaaccgca aggtagtccc agcgatgcag
      601 gagatcctgc gcatcgtagc ggataacgac ctgatactag gcaccggtca ctacccctac
      661 gaggatagcg ccattctgtt cgaggaagct cgacggctcg gggtaacccg aatggaggtg
      721 gtgcatccgg cccatatcca ctccaagcac accatcgaga acatgaaggc ccaggtcgcc
      781 agaggtgcca agatcatgct atccggcctc ggcgctatgg ccttcccgct tcacgaaagc
      841 ggcccgctgt acgccgcacg cgtcgtgaag gaggtaggtc ctgagaacgt ggtctacgga
      901 tccgacttca accaactgca gaacttccca ccatccatgg ccgctcgctg gatggtgaag
      961 ttgctgctcg cctacggctg cactaaggaa gagctatacc aagttttcca ggtcacgccg
     1021 gcgaagcacc tgggcatcga attgccccct ccgcttggca cccccgctca ccccggctgg
     1081 cctcgtgacg atagcgccaa ggcttacgtc ggccaccccc aggccgatcc gaccgtccga
     1141 atgcgatccc atgggctcta caaaccggca gagtggacaa tccccagcaa cggtgagtcg
     1201 tgattcaatt gcccagactc gaagcgtaat gtcgtggagg tagaaagaaa ggatgaagcg
     1261 tctggtcacc ttcctactgg caatcaccac cgcaacagtc ctgctcgctg gctgctctca
     1321 agctcagcca gcagccacgc cggcacccac caaggcgccg gcaagcgccg ccgagcccac
     1381 aaaagccgcc gcgcctactg tacagcccac aatcgcagca cagcctacgg ccgccccccg
     1441 ccaagaaggt tgatttcccg cagaagggca agtcgatcac gatgatcgtg ccatggccgg
     1501 cgggaggcgg tgtggacgta ggtacccggc tgctaacccc ctatctggaa aaggagttgg
     1561 gtgtacctat ccaggtagtg aacaaggcag gcgccagcgg acagatcggc gaaatggaga
     1621 tgctgaagtc caagccggac ggttacacca tctgtgcgac cctggttccc ggcaccatca
     1681 ccgtctatct ggatccagag cgcaaggcga cttatgggcg caaggacttc cagcccctcg
     1741 ccctccatgt ttccgagccg gccatcctcg ttgtcaaagg cgatagccca tacaagaccg
     1801 caaaggagtt cattgaggct gccaaggcca accctgagaa ggtgaagata tcctcctcgg
     1861 gcattatgag tccggcccat atggcgacgc tgcagttcca gaaggtcact ggctccaagt
     1921 tcgccatcgc ccagttcgac ggtgcggccc ccgccttgac tgccctgtta ggtggccacg
     1981 tggatgctaa catcggttgg ggcggcaact tcgtaggacc tgctaagaac ggcgacattc
     2041 gggctctggc tgtgatggag agtcagcgca gtgagttctt ccccaacgtc ccgacaatgg
     2101 aagaactggg gtacaaggta agcttcagtt ctgcccgagg cttctccgtg ccggccggca
     2161 caccgaaaga tgttgtggac gtcctggacg ccgcattcaa gaaggcaatc aacaacgacg
     2221 agcacaagaa gaagatacag gaggcgtctc tgcctctcaa gtacatggat gccgcagcgt
     2281 acggtaccta ctgggacgaa accgagacca taatcaagcc gatgatggct aatcctcagt
     2341 agttcaacaa ccgacatcca gcgtggtggc gactcgcatt atctgcgagg taggctcctc
     2401 ggagatcact gatgtgggcc tcgacctctc cagcgagtcg ccaccaacca agcacaacgg
     2461 tcttgcctgg ggatttcaca gtcgagagga tgagcgatgg gaagaatctg ggatgatgtc
     2521 ctcaccgaga aggacaagct ggtatatgaa gcggccggct acggtcaacg tgctggactc
     2581 ggccgtaagc cggcgattat catcatagat gtgaactaca actttgtggg ggataagccc
     2641 gagccaatcc tggagtcaat caagcgattc cacatgtcct gtggcgaggc tgggtgggat
     2701 gcggtgcgtg ggctgcagca gatcctaccc ctggcgaggg agcggaaggt cccggtcttc
     2761 tactccacgg ggaaacctag gagcggagcg caacggatct tcgccgggca gtgggcggcc
     2821 aagaatcacc gcacgggcga ggccggagag ggcgacggca accagatagt ggccgaaatc
     2881 gctccagggc ctgatgacat tctcattccg aaggagaagc cctccctctt cttcgcaacc
     2941 cccctgctga gctacctcat atatcacaac gttgacacag tcttcgtctg cggcaccacg
     3001 accagtggat gtgtgcgcgc aacggtcata gatgccttct catacaattt caaggtgggc
     3061 gtcattgagg agtgcacctt cgaccgcggc gaagtccccc acaagatcaa cctgttcgac
     3121 atgaactcga agtatgccga cgtcatgagg gtgacagaag tgatggatta tctacgctct
     3181 ctacctgccg acagatagcg gcaggagagc cggcgaggac gatgaattcg ctcatcccct
     3241 cgatcagttc cactttcgtc ctgcggaacc agagagttcg acaacaatcg gcactcgaga
     3301 tcactgaggt atgaggccag ccatgtcgaa agtcagaatc accctggctt gtggtgacta
     3361 cgaccgaact cgggcactgc tagacggacg ggtagtgccg gacggcatag acttgaacta
     3421 cctgcccctc gagccggaag agaccttctg gcgaatgatc cgtcaccagg agttcgacgt
     3481 ctccgaaatg tcacttgcca tgtacgccat agagtgcgcc cgaagcacct cgccctttgt
     3541 ggccatccca gtgttcccct cccggatgtt caggcattcg tcgctctatg ttcgacagga
     3601 ttccagcatc gaacggccag aggacctcat tgggaagcgg gttggtgtac cagagtacca
     3661 gatcacggca gcggtatggg tgaggggcat tctgaaggat cagtacggcg tcagtcccga
     3721 acaaatcgag tgggtgaccg gcggacaaga acaccctgac cgtaaggaga agattccgct
     3781 tcagccacct ccgggggttc atgtcgagca agctccgcct ggtagatccc tgaacgatat
     3841 gcttctcgat ggcgaaattg cggctttgct ggcagcccgt accccttcca tctttcgcac
     3901 gagaagcagc cagataagac ggttgttccg agactgtcga caggtggaga aggagtactt
     3961 caaccagacg ggtatcttcc cgatcatgca taccatcgtc gtccggagag atgtccatca
     4021 gcagtaccca tgggtggcct ccagcattct caaggcattc atccaggcaa aacggctctg
     4081 ctaccagatg atctccagtc ccacatcact tcgctacctg cttccatgga tggtcgccga
     4141 cgtggaggag atggtcaacc tcatgggcga agatccatgg ccggatgggc tcagccccaa
     4201 ccagaaaacc ctccaaactc tgatgggtta cctctgggac caggggctga ctccgcgccg
     4261 cctggatccc gaggaactgt tcgcgcccaa cactctggag agcttcaaga tctagcagtg
     4321 agggatccac gtatctatcg gtgtgtcatg ccccacctcc acaccgtcac gtggacgatg
     4381 cgttggtcgg aggactgctc tccatctgct atcatagtcg ccaagctagt gttgcccggg
     4441 ctttggagcc ccttcatcga gaattcgacc tttgggtgga gcagccagtg tctaccatca
     4501 aggatgtagc cgagcgcacc ggactctcag tctgtacggt ctcccacgcc atacatggca
     4561 ctcgccatgt caaaccggag actcgagagc gagtgctggc ggtggcggag cagattggat
     4621 accgccccaa caggcttgct cgggggctga agacgaagcg aacgaataca gttgggtgtc
     4681 tcgtttccca cgttcgcgtc ccgtctgtcg gtgtcttggt aactggtgcc gaggaagtgc
     4741 tggttgcgca tggatactcg cccatcctct cctgcacgca tctgcagtct tcctttgagt
     4801 tgaacgccat cgacactctg acagatctgc gtgtagacgg gctcttggca acgggatgct
     4861 ctgagatgtt cgtcaacaag atggcaggtt tcaccgaatt cccagttgtg ctggttcact
     4921 gccatccggc ctcggccacc gtggactgcg tggacatcga tgacgagcta gctatgagga
     4981 gggctacgga gcacctgttg tcgctcggtc accggcgaat cgggttcgtt ggacgggcaa
     5041 gcccacgcgc catgttcgcc gcccgccgga ctggatactc cctggcgctc ctgtcgcacg
     5101 gtatcccttt ccgggatgaa ctcatatgtg aagttcagcc tgaaccccgc gaggtgtggc
     5161 aggggatgag tcgtctacta tctgcagcgc ccagaccaac ggctctggtt gttgcccatg
     5221 cccagttggc attcgaggtc gtccgtttcc tcagagaaaa gggcgtacgc gtgccggacg
     5281 acattgcggt ggtgggcatg ggaagcgacc cctggaatga tttggtcgat ccaccgttga
     5341 ccaggatctc agtcccctct ctggatcttg ggcgagcagc cgcggacctt ctggtatcgc
     5401 ggttggaggg tagaggacct gacagacccc agtcggtgct tctcgatgcg ccgctcatca
     5461 ttggtggatc ctgcggcagc cccatggcat ccaccccggg tcgtgtcgca gggttcaacc
     5521 aggatggaag tggtgtcttc tctagtagtg tcgagaatag tgtagagtag attagtattc
     5581 tcccacgcgc caaagaggtc tcgtacacca tctgctatac tgcggccagt tttgctgtct
     5641 cgtgcagagg gaagggagag gctgatggat gaacaggaat tcctcgactc gagggaggag
     5701 atggagcaga ttctccggga ggaggtggta ggctacctgg ggctctcgga caacggcaag
     5761 ccttacgtcg taccactgaa ttacgcctac atgtccggca agatcgtctt tcactgcgcg
     5821 ctcaccggcc aaaaactcga cgccatccgc cgcaaccccc acgtttgttt taccgtcggc
     5881 cgtcaggtag gcgacgtccg cgaccacgcc aacgaccccg tgtgccatcc cgacagtgac
     5941 agtgtgatct gctatggtat ggcgaggctg ctcgaagacc atacagaacg ggaggctgca
     6001 ctaaacgcct tcaatcggcg atttaggcca agcgagcccg acctaccaat gaagcggata
     6061 gagcgttgca tggcggtcga gatcacaatc accaagatga ctggccgaag ggagagggct
     6121 cgccatcgcg catattggcg ctccaccttc tagccccctt cgcgtctagc aacgcttaga
     6181 tatctttgag tcctcagcct ttgccccgta gcacaggacg gctcaggcat cagtcgcagc
     6241 gtggtagtag tagtgaagga cgtcgccaca gacgttccgt agatcttcta ccttctcctc
     6301 ctgcatagtg tcccgtgctg ctcccagttg accactgtca tctcatcgat tcgtaccgcc
     6361 ttggctggtc tcttctgtgt catcccagtc ttgagcctag ccctctcgat tcactagccg
     6421 caggtcgatg aagaaggctg ggagctgctg gacgagatcg gcaacgtcct ctcgatgctg
     6481 caacagaaaa ctgcacgggt ggaacatccg aatcccgagc agcaccagca gtcaattccc
     6541 cgtttccgtg tcacagattc atccggaaga tagatggagc cggaccgatc ggtccggctc
     6601 cataatcctc agatcagacc cactctactt gctctggacg gtcacctgcc caccaccaag
     6661 ctggacgggc aagttggcgg tccccacctg cttccacacg ttgttgttcg cgtccatgat
     6721 cgtgatggcg aatttgtcgg gtcccttcgg attggcctgg tcgccatccg tgaggccgac
     6781 ggtgtaggag tagttgcccc aggatggggc gacgctacca gtaacacggt caatcttctg
     6841 cacggtgcat tttccactga aggtcgcctt gctcgggtcg ctcgcgaacc caaggccccc
     6901 tccctgccag ctgttgctct tgatcacgta gttgaacccg tcgcccttgc tcccatacac
     6961 gaacaccgac tggcccttgg gattgcccgt cttctgatag ttgacggtga agccgaagtt
     7021 cgccttgccg ttgagactgc cgtcgtaggg aatccagcca cctcccacaa cacgcctgct
     7081 ggggtcaggc agcgccacca tgactaccga ggatccgaca gaattggtgg tccagtagcc
     7141 attgtccgag ctgattttga cgttcacgac ccaagagtcc acgcctagcg agaccattgt
     7201 ctgtgccgtg ccgtcggcat tcactggcac accgctaatc acctgatctg gagtggtaga
     7261 caggttgctc gacttgaaga gctcgaaggt gaccttagcc ttggtcggat cacccgccgc
     7321 cccatcggcc tcctcggtta gtctcgctgc cagctgcagc tgagtggtca agctcgtcat
     7381 acttgatgtg agcgcaaaga ggcttccagt gtactcggta gcggctttct ccttagcaac
     7441 caccacggac acctgcttgg tatccgtgag tccatgcggg tctgtcaccg tcaaatcgac
     7501 cggataggtc caggcagaag cagtgaccgt accagatacc gaagcggtgc cattgaggtt
     7561 gtctgttagg gtaaggtccg caggtagccc cttggctgag aagcggagat ccgacgaaga
     7621 atcatctgga tcggtggctg acaccatgaa aagcatattg tcgctgtatt cgacttgaag
     7681 cgatgccgga gctgtgagag ttggaggttc attcgttggc cccactctta ccggaactcc
     7741 tgggaccgag gagtactctg cccgataaga atccacggct ccctgttggg cctgtccaga
     7801 tagctctcca gtactaggtt gcccagtatc atcgtaggcc aacacttcca ctctatagga
     7861 acctgtccct attccgatca cttggatgtc gtaacggcca gactgtggac cgtaacaata
     7921 gaacgtcttg gtgcttccaa ttgccaacgg ctgtgaagag tgatcgtcaa ttccgtcttc
     7981 gtagtagacg cagtcaggca tctcgtcata cttggctccc gttcgcggat ctatgcccga
     8041 acgtcggccc tgagcatctc tcaccagcaa ctctgctggt gagaagagcc taagggcaaa
     8101 cgaccccttt gaagcggcag gattgtcata ggcgctcatg gcctgtatgt aatcgttgtt
     8161 atacctgtca tacaacacca tggataacgg tacatagcga tcgattgaat ctggcttcgt
     8221 gctcttaact gcggggtcac ttatgtagaa agtatcgtct ccattcggag catcgctaac
     8281 ccccagtgcc agaacatggt ggaattcctg gacttgtagc ttcgctggaa ttctttcgag
     8341 taggtcggag atgagcgtgt tcttgtcctt atatggtttc acagcactga gattcccaat
     8401 cccaaactct cggccgcatt ccaccgctct ctcttggatg acccctttgt catccgtata
     8461 gccctttctg ttcttgaggc acgcatcaag tcttggcgga ttcacgtgaa caccgcggtg
     8521 ctgcccccag tagtccaaga tcatggccca actggtgagc gcgcatccac tgcccccgat
     8581 cgtcatattg ctcgatcccc cataggggag gctgccccag ttagcttggg gggtgctcgg
     8641 aaagccagat tgcaggaaat gttctatccc caacgccagt acaggaggag ccgaataggc
     8701 tcggccctcg attatttcga cccttagggc ccctacatta tcaccacggc aggcgctgca
     8761 gtcggctatt tcgaacgtga gttcctgatc attgcctgca atttctcccg tataaaggtg
     8821 gtcgctacga tagggaccga agttcgcctt gagcccattg atcataaggt agacgacacc
     8881 tgcccaatag gacggtgaga agcctaccca atgatcatct gttaggtacc ccgcatcacc
     8941 ccaacgcagg tactcgggtc cacctcctgg gcaactgtaa ggttcgcacc ctccccaaac
     9001 gaatactccg ctaacgcgaa tagtataggt ggtccctctg gataatctgg gcgacttgac
     9061 ttgggatccg gtcggtgaca ctgtcaagac ccccaatact cttccgacag ccgccgtttg
     9121 gacgttgacc ccagcatcca tttgggagga cggggtgaga accaattccg cggcgggcgc
     9181 tccgtcactt ccggaggttg ggagtggtac ctttgtcgga gtagggctag ttaaggcttc
     9241 gtctggccag atccccgtaa tgctaacagt ctgagcggac agatcagagg ctgctagcac
     9301 tgttacaact gccagcactc cgatgacgac tactgctagt ttcattgcct tcatctcctt
     9361 gaggctcaac ccagaccgaa ccagctagac acgggttcct ctactcgaca tcgtcgggac
     9421 tttagggcgg aaggcacgag gatgatcaaa cagggattac tagggcgaga tcgggatgac
     9481 tgcgcgctgc ctatcggctc cgcccgacac gcgtatccag cgcgcggcaa ctccattcct
     9541 tctcacaaga taaggccagc cgagttagta gcagctaact cggctggcct tatgcgccta
     9601 ccctcctaga tacctagaca ggcaccactc agcaatgcat atgctactat accccgcata
     9661 actgtcttgt caatttctat actatgccca gttgcattaa tatctgttag gacttcttac
     9721 atcctttggt caaaccgtgt ggatgtatca tggactgtcc ggtaagctgg cggagaagaa
     9781 agtactaagc tttccggcca gccacctgcc ctgcccacga cctcccattc accgccggaa
     9841 gtgataccca ccttcgtcga gttcatctac gactagtctt tctatctagg gatacagtgc
     9901 gttcagtccc tcgaaatcca accccagttg ctcacagagc tctttcatcc ggcggacgcc
     9961 ttggtgtggc actgtctcgt agagcaccca gttcggtatg tttccttgtt gactccgact
    10021 gtctcagcca gttgcttttg ggttaatctc ttcttcagcc tctccttcct gatacactcg
    10081 ccgagggttg aagtaggagc tggagttggt cggggtggat aggtccgttc gatactccaa
    10141 cagaaacctg cacgggtgcc ctaacatcgc tgctacctct cactaagtgc gtc
//
LOCUS       DATALS010000295         3698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_104704, whole genome shotgun sequence.
ACCESSION   DATALS010000295 DATALS010000000
VERSION     DATALS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104704"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..2978)
                     /locus_tag="VHS06_06605"
     CDS             complement(447..2978)
                     /locus_tag="VHS06_06605"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX2987825.1"
                     /translation="MLYCGGGRIAALAIPNSTRFLVLHLYRNAAHTAGPASGERLGAL
                     RGSRGLSDWALAHAIDLAAILLHAVFTVVFLWPVAVQGMVPAGYDLVTYFYPYKAYLG
                     EMVRQGVPPLWNPSIFMGAPLLANIQAAVFYPPDILFYLLPTTDALRISVVLHVFFAA
                     TFSYLFARWSLSLSPVAAWVCGAVFAYGGFVGAQVGHLNQLHAAVWLPLLLLCLERAL
                     SARSVAAVALGGVVLACQILAGHTQEVYYSCVALGLFALCQAAFGREGGLKDRLLPLG
                     AMLGVVGIGAAVAGVQLLPSLELAGQSYRSGGIPFGEAVGYSVHLRDLLDSLLPLYTS
                     LPYVEVAGYTGVLSLALLPAALATRRRDQLHWFFLCLAVLALLLSFGDGTPLYGVLFR
                     LVPGFDLFRAPGRWLFLYGFSVAALAGIGLDSLIRHRRAEEWTEWMGRYGLAMGVGLA
                     AFAALRLWMGTQGEEFGLPHGRVVMTWSLLLGAGVALSLAAFSRQAHRTALLLLVILL
                     MADLYQAREPMEYNRPLDANVYSGSRPILSLLAGQPPARVLTLAVEQYQMVDEDDLKA
                     RLARQSPSVDVTSYLGYTRLREIVAPNTGMSSGLSSVDGYDGGLLPTRRYAELKQTLM
                     GSREYKPDLSMRGEPGQIPDSRTLGAFGVGYLLLGPERGDPGAGWEEIGAGTGGPVRV
                     LRNRNLLPRAFVVHRAQLFGDDSQLLAALRQADLSQTVLLRDDLQFPDVEAPGVDRVT
                     VVADVPNAVDLEATLEKPGFLVLSDSYYPGWKVYVDGSESHLLRADHAIRAVQLPAGS
                     HQVRFVYDPLSFKAGLGLSAMGLLAVLVILTAGRRWRSAEASAGV"
     gene            3120..>3698
                     /locus_tag="VHS06_06610"
     CDS             3120..>3698
                     /locus_tag="VHS06_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX2987826.1"
                     /translation="MEDTGRRFDGVKIAAYCESILKKVGAPAEHAHMVASSLIAANFR
                     GVDTHGVTRLPIYVERIQAGLMDAKASGTVVSEGPASMVYDGQNGMGQVVGTKAMRLC
                     LDKAEKSGVAFTTVRNSNHFGTAAYYAMMALERDMIGFSITNSPPVMAPWGGKKGFLG
                     TNPLAIAVPAGEERPFIFDMATSVVARGAVILA"
BASE COUNT          782 a         1224 c         1123 g          569 t
ORIGIN      
        1 gtctgccgaa gcctcggccg gtgtttgatt cgacggccca tttgtcctac aatcatcatc
       61 aagctcccct tcgaccaatc cgtgagccag aggccgcggc tgtcgctcac caactcctca
      121 gccggaaagg tttgtacgtc cgatgctccg ccgccgctcc ttgggccgct cacccgttcc
      181 gagccagttg cacccttcgg ggcagcagat ctgccctgct ccccgatcga aggttgtgcc
      241 gttatgtata gtcccagcca cggactcacc actccctgga cggacacgga caccaggaga
      301 agtagggaga ggaatacaca agcccgggca cggtccaacg acagagtact ccgaaagaaa
      361 gcagttggcg gcggaacggc aggaatggaa agggtggttc tcgaaggaga gcttgatgat
      421 gattgtagga caaatgggcc gtcgaatcaa acaccggccg aggcttcggc agaccgccac
      481 ctcctacccg ctgtcaggat caccagcacc gcgagaagac ccatggccga gagcccgagg
      541 ccagccttga aggatagcgg gtcgtagaca aaacggacct ggtggctgcc ggcgggcagt
      601 tgcaccgccc ggatggcgtg gtccgccctg agcagatggg actcgctccc gtccacgtac
      661 accttccagc ccgggtagta ggagtcggac agcaccaaga agcccggctt ctcgagcgtc
      721 gcctccagat cgacggcatt ggggacgtcc gccaccacgg tcacacggtc caccccgggc
      781 gcctccacgt ccgggaactg caagtcgtcc cgcagcagca ccgtttgact caggtctgcc
      841 tgccggaggg ccgccagaag ttggctatca tcgccgaaca gttgtgcccg atgaaccaca
      901 aaagccctgg ggagaaggtt tcggtttcgc aggacgcgca ccgggccgcc ggtccccgcg
      961 ccgatctcct cccaacccgc tcccggatct cctctctctg gaccgagcag caggtaaccc
     1021 actccgaaag cccccagcgt acggctgtcc gggatttgcc caggctctcc tctcatgctc
     1081 aggtccggct tgtactcccg ggaccccatc agcgtctgct tcaactccgc gtatcgtcga
     1141 gtcggaagga ggccgccgtc gtagccatcg acgctggaca ggccggagct catgcccgtg
     1201 ttgggagcca caatctccct cagccgggta tagccgaggt agctggtaac gtcgaccgac
     1261 ggagactgtc gcgccagtcg ggccttcaag tcgtcctcgt ccaccatctg atactgttcg
     1321 acggccaggg tgagcacgcg ggccggcggt tgcccggcca ggagcgagag tattggccgc
     1381 gatcccgagt acacatttgc atctagcggt cggttgtact ccatcggctc ccgagcctgg
     1441 tagagatctg ccataagaag gatgaccaac agcagaagcg cagtcctatg ggcttgacgg
     1501 ctgaaagcgg caagggagag agccaccccc gcgccgagaa gcaacgacca cgtcatcacc
     1561 acacgcccgt gaggaaggcc aaactcctct ccctgagtcc ccatccagag ccgcagcgcg
     1621 gcaaatgccg ctaaccccac tcccatggca aggccgtagc gtcccatcca ctctgtccac
     1681 tcctccgcgc gacggtggcg aatgagggag tccagaccga taccagcgag ggccgcaacg
     1741 gagaagccat acaggaatag ccagcgcccc ggagcgcgga acagatcgaa cccgggaacc
     1801 agccggaaaa ggacgccgta aagtggagtg ccatcgccaa agctcaacag cagcgccagc
     1861 actgccaggc acaggaagaa ccagtgaagt tggtcgcggc ggcgtgtcgc tagagcggcg
     1921 ggcaggagcg ccagggagag cacaccggtg taaccggcta cctcgacgta gggcagcgac
     1981 gtatagagag gcagaaggct gtccagcaga tcgcgaaggt gtacggagta accgacggct
     2041 tccccgaagg gaataccccc tgaacggtac gattgaccgg ccagttccag actgggaagc
     2101 agttgcacgc ccgccacggc cgcgccgata cccaccactc ccagcatcgc acccaggggt
     2161 agcaggcggt cctttagtcc cccttcccgc ccaaaggcgg cctggcagag cgcgaagagc
     2221 cctagggcga cgcaggagta gtacacctcc tgggtgtgcc ccgccagtat ctggcatgcc
     2281 agcaccaccc ctcccagcgc aactgcggcc acgcttctgg cggatagcgc tctctccagg
     2341 cagagcaaca gcaatggaag ccacacggcg gcgtgaagct ggttgagatg cccaacctga
     2401 gccccaacga agccgccgta ggcgaagacc gccccgcaga cccacgccgc aacaggagag
     2461 agtgacagtg accacctagc aaatagatag ctgaaggtcg cggcaaaaaa gacgtgcagc
     2521 acgacgctga tccgcagggc atccgtggtg ggcagcaggt agaacagaat gtctggaggg
     2581 tagaagaccg cggcctggat gttggccagt agcggcgccc ccatgaagat cgatgggttc
     2641 cacagcggcg gcaccccctg cctcaccatc tctccgagat aggctttgta ggggtagaaa
     2701 taggtaacca gatcgtagcc cgcgggcacc ataccctgca ccgccactgg ccagagaaag
     2761 acgacggtga agacggcatg cagcagaatc gcggcgagat cgatcgcatg ggccaacgcc
     2821 cagtccgaca atccacgaga gccgcgcagg gcacccaaac gctccccgga ggcgggcccg
     2881 gcagtatgcg cggcgttgcg atagagatgc aagacgagga acctcgtgga attggggatg
     2941 gcgagagcgg caatccgccc accgccgcag tatagcatgg cccagccacc cgcgacggtt
     3001 agccaatctc ccgtgtctct gctatgatat tcggcggccg ctgcggccaa acgggaacca
     3061 tgtcccccaa cccctggttc cctaccactc gatttacttt ctgattggag gcccatccaa
     3121 tggaagacac cggccgtcgc tttgacggtg tgaagatcgc tgcctactgc gagtccatcc
     3181 ttaagaaggt gggagcgccc gccgagcacg cccacatggt ggcatcctct ctgattgccg
     3241 ccaacttccg cggcgtcgac actcacggcg tcacccgcct gccgatctac gttgagcgga
     3301 tccaggcagg gctcatggac gcgaaggcct ccggtacagt ggtctcggaa ggccccgcat
     3361 ccatggttta cgacggccag aacggtatgg gccaggtggt gggcaccaag gccatgcggc
     3421 tctgcctcga caaggcggag aagtccggcg tcgcgttcac gactgttcgc aactccaacc
     3481 acttcgggac cgcggcgtat tacgccatga tggccctgga gcgcgacatg ataggcttct
     3541 ccatcacgaa ctccccgccg gtcatggccc cgtggggtgg caagaagggc ttcctgggaa
     3601 ccaaccccct cgccatcgcc gtccctgccg gcgaggagcg ccccttcatc ttcgatatgg
     3661 ccaccagcgt cgtcgcccgc ggtgcggtga tcttggcg
//
LOCUS       DATALS010000296         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_213741, whole genome shotgun sequence.
ACCESSION   DATALS010000296 DATALS010000000
VERSION     DATALS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213741"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1654
                     /locus_tag="VHS06_06615"
     CDS             <1..>1654
                     /locus_tag="VHS06_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2987827.1"
                     /translation="LPSFIREGDGFDAAAVVASNHEGPVQVTVSVDGNQTGSWNVRLA
                     AGANQVSVPQQSLSLGFHSVEVQISGDGDTVQENNRAQGFVVVKPKGRVLVVEGHPNA
                     DGYLLRQLEGSGLQVDKLPASQLPIQMPQLVGYDGIIMDDVSGPSLSLDQVKTLQSYV
                     RDQGRGLLVLGGKNSYGLGDYLSTPLEEMLPVSSEPPASKQRGNMALILLIDKSGSMD
                     DSSNGVTKIAMAREAAIQATKELKPDDQIAVIAFDTDPQWIVPPQKVGNNLNSIEDRI
                     SSLDSSGGTDIYTALQTAFTTMQGLQATTKHITLLTDGQSWKGPYQQLMQQMQQARVT
                     LSTIGVGGDADKDWLSELATLGQGRFYFTERFTDIPKIVFREVNAATRIAQMEGNVGP
                     QFSAPSPILRGIGKDALPMLNGYVATKPKDGATVVLKSDQGDPLLAQWQYGLGRVVAW
                     TSDTQGIWSSQWVTQPQYAHIWDQAVRWTMAPPIDRGLQLSTEVDGAHVLLTADSIDL
                     NGQFVNLADTRAILWEPDGSQQNLAMQESAPGRYQANLPADKA"
BASE COUNT          359 a          503 c          534 g          258 t
ORIGIN      
        1 ctaccctctt tcatccgaga gggcgacggg ttcgacgccg cagccgtcgt ggccagcaac
       61 cacgagggac cggtgcaggt cacggtctcc gtggacggca atcagaccgg aagctggaat
      121 gtccggcttg cagccggggc aaaccaggtg agcgttccgc agcagtcgct gagcctcggc
      181 ttccactcag tggaggtgca gatctcaggg gatggtgaca ccgttcagga gaacaaccgg
      241 gcccaaggtt tcgtggtggt gaagccgaaa ggacgcgtac tggtggtgga ggggcacccc
      301 aacgccgacg gctacctgct ccggcaactg gagggatcgg gtcttcaggt ggataagctc
      361 ccggccagtc aactgcctat ccagatgcca caactcgtcg gatatgacgg catcatcatg
      421 gacgacgtct ccgggcccag cctctccctg gaccaggtca agacgctgca gtcctacgtg
      481 cgcgatcagg gtcgcgggct gctggtgttg ggaggaaaga actcttacgg gctcggcgac
      541 tacctcagca cgccgctgga ggagatgctg ccggtgtcct cggagcctcc cgcctccaag
      601 cagcggggca acatggcgct gatactgttg atagacaagt ccgggagcat ggacgactcc
      661 agcaatggtg tgaccaagat cgccatggcc agggaagcgg cgatccaggc taccaaggag
      721 ctgaagccgg acgaccagat cgcggtaatc gccttcgaca ccgaccccca gtggatagtg
      781 ccgccccaaa aggtgggcaa caatctgaac tccatagagg acaggatctc cagcctggac
      841 tccagcggcg ggacggatat ctacaccgca cttcaaacgg cgttcacgac catgcagggg
      901 ttgcaggcta ccaccaagca cataacgctg ctgacggacg gccagtcgtg gaagggcccc
      961 taccagcagt tgatgcagca gatgcagcag gctcgagtga cgctatccac catcggggta
     1021 ggaggagatg ccgacaagga ctggctctcc gagttggcta ctctgggcca gggccgcttc
     1081 tacttcaccg agcgcttcac cgacatcccg aagatcgtct tcagggaagt gaacgccgcg
     1141 acgaggatcg cccagatgga ggggaacgtc gggccacagt tctccgcccc cagccccata
     1201 ctgagaggga tcggcaagga cgcgctgccg atgctgaacg gctacgtggc aaccaagccg
     1261 aaggacgggg ccaccgtggt actgaaatcg gaccagggcg acccactgtt ggcccagtgg
     1321 cagtacgggc tcggcagggt ggtagcgtgg acctcggata cccagggaat ctggagctcc
     1381 cagtgggtca cacagcctca gtatgcccat atctgggatc aggctgtccg ctggacgatg
     1441 gccccaccca tcgaccgagg gttgcagctg agcacagagg ttgatggagc gcacgtgctg
     1501 ctgaccgccg acagcatcga cctgaacgga caattcgtga acctggcgga taccagggcc
     1561 atcctctggg aaccggatgg cagccagcag aacctggcta tgcaggaaag cgcacctggc
     1621 cggtaccagg ccaacctacc ggccgacaag gccg
//
LOCUS       DATALS010000297         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_214049, whole genome shotgun sequence.
ACCESSION   DATALS010000297 DATALS010000000
VERSION     DATALS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214049"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VHS06_06620"
     CDS             complement(<1..198)
                     /locus_tag="VHS06_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987828.1"
                     /translation="MVYVPGSPTEATTVGVYRQLLEEEGYPFELVEASTLAFDTAQSL
                     KKRFVALIIPEDVNRSLETSVG"
     gene            683..1678
                     /locus_tag="VHS06_06625"
     CDS             683..1678
                     /locus_tag="VHS06_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX2987829.1"
                     /translation="MRKISVGRLTLGLILAVLGIALLLDINYGRDTLQTVVKYWPVAL
                     ILLGLEYVVASRDPESTARVSIGSIIAMAVVLCLAWVYVEAPVSFLGFQGFGFSYPGQ
                     EEYVIELPVNEAFGTASTRVNVEAIHDVTITGSADNSVVGTARITVRARTIADAKRVA
                     EQLRVVGRPSGSTLYIEISKPSDLTRFISIQPSFVLTMPSTGSVTSNTISGDTSIAGI
                     TGDVSVDSISGNILVDGAPSSVSANAISGNVHVTLNSGMKSVYAKTISGEILINAPEG
                     TGGNMTFSSVSGNVETRLSGIQVTSSPGRRTASGRFGTGDTTIQLNTVSGGAVIR"
     gene            complement(1675..2373)
                     /locus_tag="VHS06_06630"
     CDS             complement(1675..2373)
                     /locus_tag="VHS06_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX2987830.1"
                     /translation="MDIKERAPAIDAVLFDLDGTLLDTTEAIISSLKHTVHEFTGRTP
                     ELSELRPYMGLPLADTFACLTPGRVQEACDCYVEHNLAVHKDLVRAYPGVNCTIEALR
                     RCGIKRGLVTSKRRRTAQLGLEIAGLAGAFDAMVFYGDTEKSKPDPEPVMLALKLLGL
                     DTAEGRVIFVGDSPWDVRAARNASPLGPGLVIMAAAVTYGATAAEVLKKENPDYLLDS
                     ITQILPLCGCPSET"
     gene            2446..>3485
                     /locus_tag="VHS06_06635"
     CDS             2446..>3485
                     /locus_tag="VHS06_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987831.1"
                     /translation="MLQTLRPEDLYKFRNPGDAHLSPDGSRVVYVISQADKKTDRNLT
                     SIWVASTGGGEPSRLTNSGKDRSPRWSPDGRRIALISERSGKSQIWILDVAGGEAWHL
                     STDETVRGVPVWSPDGRSIAFTGRAFDKADDWTPYPGAPVWDRERAISQAEQALSGKT
                     PKDDEPKVSDVKVVTRFRYRFDGVGYFGDLRSHIFLVDVPESQEDSAGARANVRRITK
                     GDFDHDAPSFSPDGKYLVFSATRRDDADYLQKSDLWQAEIATSRLTLLMDGAGPSNQP
                     RWSPDGAKVAFVGHDNSYGGSTSPSLWVKEPGRHARNLTGSMDRPVGNPVSSDMRQDG
                     GTTFQWVDNDTI"
BASE COUNT          732 a         1020 c         1057 g          676 t
ORIGIN      
        1 gccaacgctg gtctcaaggg acctgttgac atcctcgggg atgatcaagg caacgaaccg
       61 cttcttcagg gattgggccg tgtcgaacgc cagagtggac gcctccacta gttcgaaagg
      121 gtacccctcc tcctcaagca gctgccggta gacgccgacc gttgtggctt cggtggggct
      181 accggggacg tagaccaatc cgaccctcag gacctgtccc atcgggaggt caagagtgga
      241 ctcgggcgac acattaggaa ccaacgtcac gctctcgtgt ccagggaagg caaccgtagc
      301 tactattgaa gcagcaatga gcgcgagagc ggccttgcgt ctccaagtga atagcagagc
      361 gatgccaccc ccaaatatcc tatgagcaag cggacgggca gcattagcgt ttcagcggtt
      421 gacaaggata atccgtggca agggggcaaa agagaggggg tgatgtgggt ttggggttca
      481 cctctaaggg cgggtgaatc gctagtaccg acattcccaa atcacccccg gcagtcaggg
      541 gcccggtgga agggttaccc ttcgcgagaa gattcgaggc cgactcgtta ccctccttca
      601 actcctgcgt attaccgctc ggtagtcaaa ggagcgcctg tccaggtacg acgtttcggg
      661 tgccccaagg aggatctcga aaatgcgaaa gatcagcgta gggagattga ccctcggcct
      721 tatcttggcg gtactcggaa tcgcacttct actggacatc aactacgggc gcgacactct
      781 gcagactgtt gtgaagtact ggccggttgc gcttatcctc ctagggcttg agtacgtggt
      841 tgcctctcgc gatcctgaaa gcacagctcg agtgagcatt ggctccataa tcgcgatggc
      901 tgtggtcctt tgtcttgcat gggtctacgt cgaagcccca gtctccttct tgggctttca
      961 gggctttggc ttcagttacc ccgggcagga ggaatacgtg atcgagttgc cggtcaacga
     1021 ggcattcggc accgcgtcaa cccgggttaa cgtggaggcc attcacgatg tcacgatcac
     1081 cggaagcgct gacaactcgg tagtagggac agccaggata acggttcggg cgcggacgat
     1141 tgccgacgcg aagcgtgtgg ccgaacaact cagagtggtt gggcgtccgt ccggctcgac
     1201 cctttacatt gaaataagca agccgagtga cttaaccagg tttatatcaa tccagccgtc
     1261 tttcgtcctc acgatgccgt cgaccggtag cgtcacctca aataccatct caggcgacac
     1321 ttcgatcgca gggatcacgg gcgatgtcag tgtcgacagc atctcaggca acatcctcgt
     1381 ggacggcgcg ccgtcctcag tttcggccaa cgccatctca ggcaatgtcc acgtcactct
     1441 caatagcggc atgaagagcg tgtatgccaa gacgatcagc ggcgaaatcc ttatcaacgc
     1501 gcccgagggc acgggcggca atatgacctt cagttcagtc agcggcaacg tggagacgag
     1561 actatcggga atccaggtca cgagcagccc cggcaggcgt acggcctcag ggcggttcgg
     1621 gacgggagat accaccattc agctgaacac ggtcagcgga ggcgcggtaa tccgttaggt
     1681 ttcggatggg cagccgcaca ggggcagtat ctgggtaata gagtccagca ggtagtcagg
     1741 gttctccttc tttagcactt ccgcggcggt ggccccgtag gtcaccgccg ctgccattat
     1801 cacaaggccc ggccccagcg gcgacgcgtt cctggccgcc ctcacatccc acgggctatc
     1861 gcccacaaag atcacccggc cctcggcggt atccagacca aggagtttga gcgcgagcat
     1921 gaccggctca ggatccggct tggatttctc ggtgtctccg tagaagacca tggcgtcgaa
     1981 tgccccagcc agccccgcga tctccaaccc caattgagcg gtccgccgcc tcttggaagt
     2041 cacaagcccc cgctttatgc cgcagcgccg cagggcttct atggtgcagt tgactcccgg
     2101 gtatgcccgg accaggtctt tgtgcacggc taaattgtgc tctacataac agtcgcaggc
     2161 ctcttggacc ctacctgggg ttaggcaggc gaaggtgtcc gccagaggaa ggcccatgta
     2221 cgggcggagc tctgagagtt caggagtccg tccggtgaac tcatggacgg tgtgcttgag
     2281 ggaactaatg atggcctctg tcgtatccag aagcgtccca tccaggtcaa ataacacagc
     2341 gtcgatggcc ggtgctcgct ctttgatatc catgagacta tggtttcccc gaaggagaag
     2401 cgggttcctt cattgaagtc acacgcaaat cagggagggg atagcgtgct gcagactctg
     2461 cgacccgaag acctgtacaa gttcagaaat ccaggcgacg ctcacttgtc gcccgacggc
     2521 tcaagggtcg tgtatgtcat atcgcaagcc gacaagaaga ccgaccgtaa ccttacgtcc
     2581 atctgggtgg cgtccaccgg cggaggggag ccctcccggc tgaccaactc aggcaaggac
     2641 cgctctccac gctggtcgcc cgacgggaga aggatcgctc tcatctcgga gcggtccgga
     2701 aagagccaga tctggatact cgatgtcgca ggcggcgagg cttggcacct ttcgaccgat
     2761 gagaccgtaa ggggtgtgcc cgtctggtcg cctgatggca ggtcaatagc cttcacgggc
     2821 agggcctttg ataaggcaga tgattggact ccgtatcccg gggctccggt gtgggacaga
     2881 gagcgggcca tctctcaggc ggagcaggcc ctgtcgggca agactcccaa ggacgacgag
     2941 cccaaggtct cagacgtcaa ggtagtcacc cggttccggt accggttcga tggggtgggg
     3001 tatttcggcg atctccgctc gcacatcttc ctggttgatg tgcctgagtc gcaagaggat
     3061 tctgccggag ccagggcgaa cgtgcgccgc atcaccaaag gcgatttcga ccacgacgcc
     3121 ccctcctttt cgcccgacgg gaagtacctg gtattctcgg cgacccgcag ggatgatgcc
     3181 gactatcttc agaaatccga cctatggcag gcggagattg cgacgtcgcg attgaccctg
     3241 ctcatggacg gagcaggtcc ctccaaccag cctagatggt ctccggacgg ggccaaggta
     3301 gccttcgtag gccacgacaa ctcctacggt gggtcgacct cgcccagcct ttgggtgaaa
     3361 gagcctggaa ggcatgcccg aaacctgact gggtccatgg accgccccgt cggcaacccc
     3421 gtgagctcgg atatgcgcca ggacggggga accacgttcc agtgggtaga caatgacacc
     3481 atcat
//
LOCUS       DATALS010000298         3756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_387937, whole genome shotgun sequence.
ACCESSION   DATALS010000298 DATALS010000000
VERSION     DATALS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387937"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1060
                     /locus_tag="VHS06_06640"
     CDS             83..1060
                     /locus_tag="VHS06_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX2987832.1"
                     /translation="MGVDVASETERGVLSILSQDNSYFSSGGFYPSGGITTIDQAALN
                     ATNAGFPGLRIAVEIRRILQQEADLELYAHYETMLNSYLPCSQVTILCLFDRAACSPA
                     TLATALKTHPTAILREHCCQNLYYEPPELLFGDVEANRIDWMISRIRQAREAGGALEE
                     ANAELLQTMEERAEELSIEIERRAEIEEALLEMNHTLKAMYEITPVAITALDTHNRVT
                     LWNPAAERMMGWAQHEVRGLNYPTTPRPAFGDFGALKAAVMGGSGFANISADVTRRDG
                     TIIQVNLSASPLRDGQGNINAVIMVMSDPIELEMAEPMRSKNGHSHAVA"
     gene            1094..1606
                     /gene="dcd"
                     /locus_tag="VHS06_06645"
     CDS             1094..1606
                     /gene="dcd"
                     /locus_tag="VHS06_06645"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX2987833.1"
                     /translation="MAVLTRSEILRLIHDGTIKIDPFDEGHVGPASIDLHLDNKFRVF
                     RALPHVHAVTEDADYEDVTELIEVKDSFLLLPGQAVIGITVEKITLPDDICGWLEGRS
                     RFARIGLMVHMTAGFMQPGISNKQVLEMINVGPIPLGIYPGTAVCQFIFEECRGHARY
                     EGRFQNQVVP"
     gene            complement(1784..3292)
                     /gene="ftcD"
                     /locus_tag="VHS06_06650"
     CDS             complement(1784..3292)
                     /gene="ftcD"
                     /locus_tag="VHS06_06650"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX2987834.1"
                     /translation="MAGLVECVPNFSEGKDAAVVDAIVAAMAAVPGIRLLDRSMDAGH
                     NRSVVTILGSPEAVGEAAFRGVAEAARLIDLRSHRGEHPRMGAADVVPFVPVRGVSTE
                     ECVELARRVAERIGRELSIPVFLYEAAATRPERKNLADVRAPQFEGLSELIGTDPSRD
                     PDYGPRAIHPTAGAVAVGARPLLVAYNVYLNSSDLSVAKAIARAVRNQTGGLRYVKAL
                     GIAPNEEGRVHVSMNLVNTAGTPIHRVVEMIRSEAARYGVSVAESEVVGLVSADALLD
                     AAEHYLQLNRFSRSQVLEYRLLEDAAPSGLLDETVAGFAGRVASSAAVPGGGTVAALM
                     GALAAALVAMVARLTIGRKRFADVEEKMKELEARGEAVRERLQRLAEADSAAYLEYMD
                     AAKLAKSGGAKAQARLQAASLRAAEVPLESARAALEAMEIARDGCRLGNPVARTDACV
                     AVLGGYAAVMGAGLNVKVNLPSLSPDQHPETLEAEVASLEERAKAILAEVVP"
     gene            complement(3400..>3756)
                     /locus_tag="VHS06_06655"
     CDS             complement(3400..>3756)
                     /locus_tag="VHS06_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987835.1"
                     /translation="EDYQVQVWDDAAERYVAKAVFSGGARDQFSLALRLSFALATLPQ
                     ELGTTPGFLFLDEPLSSFDVPRTEALVRLITSGQVAANFSQLFVISHNRSFDLGSFPY
                     RIVMRDGRVVESNLPQ"
BASE COUNT          686 a         1327 c         1140 g          603 t
ORIGIN      
        1 agcggggatc aacgctggag agcagtgcat ctacgtggcc gacgagcacg cagcctagac
       61 agcctgtctg gctctggagg ccataggggt ggacgtcgcc tcggagacag agcgtggagt
      121 gttgagtatc ctgagccagg acaacagcta cttcagcagc ggggggttct accccagcgg
      181 agggattacc actatagatc aggcagctct caatgcaacg aacgccggtt tcccgggcct
      241 gaggatagct gtggagatcc gccgcatcct ccagcaggag gccgatctgg aactgtatgc
      301 ccactacgag acgatgctca acagctacct cccctgctcg caggttacca tcctctgcct
      361 cttcgatcga gccgcttgct cacccgctac cctggccacc gccttgaaga cccatcccac
      421 ggcgatcctg agggagcact gctgccagaa cctctactac gagccccccg agttgctgtt
      481 tggcgacgtc gaggcgaaca ggatcgattg gatgatctcc cggatacgcc aggctcgaga
      541 ggccggcggg gcgctcgaag aggccaacgc ggaactcctg cagacgatgg aggagagggc
      601 ggaagagcta tcgatagaga tcgagcggcg agcggagatt gaagaggcgc tgctggagat
      661 gaaccacacc ctcaaggcga tgtacgagat cactccggtc gccatcacag ccctggacac
      721 acacaacagg gtgaccctct ggaaccccgc ggccgaaagg atgatgggct gggctcaaca
      781 cgaggtgcgc gggctcaact accccaccac acccagaccc gccttcggcg acttcggcgc
      841 cctcaaggcc gccgtgatgg gcggcagcgg gttcgccaac atcagcgccg acgtaacccg
      901 aagagacggc actatcatcc aggtcaacct ctccgcatcc ccactccgag atggccaggg
      961 caacatcaac gcggtcatca tggtgatgtc cgacccgatc gagctggaga tggcggagcc
     1021 gatgaggtcc aagaacggcc actcccacgc tgtggcatag acgctgcttc agccaggttg
     1081 gaggtgatcg gccatggcag tgctcacccg ctctgagatc ctgcgtctca tccacgacgg
     1141 gacgatcaag atcgacccat ttgacgaagg ccacgttggc cccgcgtcca tcgaccttca
     1201 tctggacaac aagttcaggg tcttccgggc cctcccccac gtccacgccg tcaccgagga
     1261 cgcagactac gaggatgtca ccgaactgat cgaggtgaag gacagcttcc ttcttctgcc
     1321 aggacaggcg gttattggga ttacggtgga gaagatcacc ctccccgacg acatctgcgg
     1381 gtggctggag ggcaggagcc gcttcgcccg catcggcctg atggtccaca tgaccgccgg
     1441 cttcatgcag ccgggcatct ccaacaagca ggtgctggag atgataaacg tggggcctat
     1501 ccccctgggc atctatccgg ggaccgccgt gtgccagttc atcttcgagg aatgccgcgg
     1561 ccacgcccgc tacgagggcc gcttccagaa ccaggtggtc ccgtagagac gggcatgtgc
     1621 gccgctgctg gatgccgtcc accgagccat ccgtagggac cggcgtctct gccggcccgc
     1681 gcgacgcagg atgtgggtgc ggtacgcgtt tagcgacggc ccttgtccat ggcgtaaccc
     1741 gggcggttgc caccatcgcg cgggccggca gggacgccgg cccctacggg accacctcgg
     1801 cgaggatcgc cttggcgcgc tcttccaggg aggcgacttc ggcttctagc gtctccgggt
     1861 gctggtccgg cgacaacgag ggtaggttca ccttgacgtt cagccccgcg cccatcaccg
     1921 ccgcgtagcc ccccagcacc gccacgcagg catcggtacg agcgacgggg ttacccagcc
     1981 ggcagccatc tcgggcgatc tccatcgcct ccagcgccgc tcgcgcgctc tccagaggca
     2041 cctcggcggc gcgcaggcta gccgcctgca gtcgggcctg agccttggct ccgccactct
     2101 tcgccagctt cgccgcatcc atatactcta gatacgctgc ggaatcggcc tccgccagcc
     2161 gctgcagccg ctcgcgaacc gcctcgcccc tggcctccag ttccttcatc ttctcttcca
     2221 cgtcggcgaa gcgcttcctc ccgatggtga gcctcgccac catcgccaca agcgccgccg
     2281 ccagcgcccc catcagggcg gcgacggtgc cgccccccgg cacggccgcg gacgaggcga
     2341 cccttccggc aaaaccggcc accgtctcgt ccagcaatcc ggagggcgct gcatcctcga
     2401 gcagcctgta ctccagcacc tgactccggg agaaacggtt caactgcaga tagtgctctg
     2461 ccgcatccag cagcgcgtcg gccgagacca gccccaccac ctccgactcg gcgaccgaga
     2521 ctccgtagcg cgccgcttcc gaacggatca tctccacaac ccggtggatc ggggtgccgg
     2581 ccgtattcac gaggttcata gagacgtgaa cgcgaccctc ctcgttgggc gcgatcccca
     2641 gcgccttcac gtagcggagt ccacccgtct ggttgcgcac cgccctggcg atagccttcg
     2701 ccaccgagag gtcggagctg ttcaggtaga cgttgtaggc caccagcagc ggcctggcgc
     2761 ccacggcgac ggctccggca gtcggatgga tggctcgagg gccgtagtcc ggatcgcggg
     2821 acggatcggt gccgatcagc tcggagagcc cctcgaactg cggagcgcgc acatcggcca
     2881 ggttcttccg ctccggccgg gtcgcagccg cctcgtatag gaagacgggg atggaaagct
     2941 ccctcccgat cctttccgcc accctccgcg cgagttccac gcactcctcc gtggagacgc
     3001 cgcgcacggg gacgaatgga accacgtctg ctgcgcccat cctcgggtgc tcgcccctgt
     3061 ggctccgcag gtcgatcagc ctggcggcct ccgccacgcc ccggaaggcc gcctcgccca
     3121 cggcctccgg gctgccgagt atcgtcacga cagagcggtt atggcccgcg tccatactcc
     3181 gatccagcag ccggatgccc ggcaccgccg ccatcgcggc gacgatcgca tccacgaccg
     3241 cggcgtcctt accctcgctg aaattgggca cgcactccac gagtccggcc atcaactatc
     3301 tccttcaatc tcgatctaca gggatcgacg ctgtccaccg atcccgcccg gacggcccgt
     3361 tcccagcgct ccggccacta ccgtccccca ttctctcacc tactgcggca ggttgctctc
     3421 caccaccctg ccatccctca tcacgatccg gtaggggaag ctccccaggt cgaaggagcg
     3481 gttgtgggag atgacgaaga gctgactgaa gttcgccgcc acctgcccgg aggtgatcag
     3541 cctcacaagc gcctcggtcc gaggtacgtc gaaggagctg agtggctcgt ccaggaagag
     3601 gaagcccggt gtcgtcccca actcctgagg cagggtggcg agagcgaagg agagccgcag
     3661 cgccagggag aactggtcgc gagccccacc cgagaacacg gcctttgcga cgtaccgctc
     3721 ggcggcatca tcccacacct gtacctggta gtcctc
//
LOCUS       DATALS010000299         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_329576, whole genome shotgun sequence.
ACCESSION   DATALS010000299 DATALS010000000
VERSION     DATALS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329576"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VHS06_06660"
     CDS             <1..489
                     /locus_tag="VHS06_06660"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX2987836.1"
                     /translation="EGWILLPSIALIAIVTVAIGLAASMGGRARFAHWAAGTGHLKDP
                     RILVSVMALTVVGLGLGLVVNLAVLQALDLPAGTAIVLTVLVSGYAAGLLPAGPGQLG
                     VFELAISAPLMAQGFPPASAVAAALTLHLVMLTMLLLGGALSMPVSLRSGSERKAAGE
                     RQ"
     gene            527..>1920
                     /locus_tag="VHS06_06665"
     CDS             527..>1920
                     /locus_tag="VHS06_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX2987837.1"
                     /translation="MRIASRSTLIVAVALLLGLSAALIPLKTAAQQEGPAFAVANQAF
                     WDYYQKRGGVRSLGYPISRQFTLRGYQVQLFQRGVLQLMPDGSVSSMNLLGEELLPYS
                     SFNGSLVPSPQRQLMDSAPSPADPDYAGKAVAFVQAYAPDQWEGLPVNFGRTFMSTVR
                     YEDAFPAGDGNPTLLPLLNLEMWGLPTSEPARDPNNGNFVYQRFQRGIMQFDATTGTT
                     QGLLLGDYLKAIMTGQGLPADIAARAVSNPLYGQYDAANHRGPLHPELLSATNLDGAF
                     QPGWGDPRCGVVVAGPCTNDPNYVARSLASLGAGSWYDFSNGSAWPDTGRAEVVRPGA
                     KLSALADRARANPGTAWLVGNEPNVPGQDDLKPSEYADFLYRVAAAIKGADPTAILVG
                     PNSLNWEDTCTSCPGFTKGRSWSEEFVTNYRQRYGALPLDVWGIHAYSLDWENLPLTH
                     ATAVEAQIAGAGEW"
BASE COUNT          344 a          628 c          603 g          345 t
ORIGIN      
        1 gagggctgga tccttctgcc ctccatcgcc ctcatcgcga ttgtcaccgt ggcaatcggg
       61 ctcgcggcga gcatgggcgg ccgagcgagg ttcgcccatt gggctgcggg aacaggccat
      121 ctgaaggacc ctcgcattct cgtgtcggtg atggcactca ccgtagtcgg cctggggctg
      181 ggcctcgtgg tgaacctcgc ggtgctgcag gccctggacc taccggccgg aaccgcgatt
      241 gtacttacgg tgctggtgag cggctacgct gcgggactgt tgcccgccgg ccccggccag
      301 ctgggcgtct tcgaactggc tatctcggca ccgctgatgg cacaaggttt cccgcccgca
      361 tccgcggtcg ccgcagccct gaccctgcat ctggtgatgc ttaccatgct gctgttgggc
      421 ggagcactct cgatgccagt cagcctgcgc tctggatctg aaagaaaggc tgctggggaa
      481 agacagtgaa atggggccgt cccgcggcaa caacagggag aagtggatga ggattgcatc
      541 acgatcgaca ctaatcgtcg ccgttgcgct gctgctcggt ctgagcgcgg cgttgatacc
      601 actcaagacg gcggcacagc aagagggtcc ggccttcgcc gtcgccaacc aggctttctg
      661 ggactactat cagaaaaggg gcggcgtgcg tagcctcgga tatcccatct cccgacagtt
      721 cacgcttcga gggtaccagg tgcagctgtt ccagcgggga gtcctccaac tgatgcccga
      781 cggtagcgtc tcatccatga acctgttggg cgaggaactg ctcccctact ccagcttcaa
      841 cggctcgcta gtcccctcgc cccagcgcca actgatggat tcagccccat cccctgccga
      901 tcccgactat gcaggaaagg cggtcgcgtt cgtgcaggcc tatgcccccg accagtggga
      961 agggctgccc gtcaacttcg gccgcacatt tatgtcaacc gtcaggtatg aagacgcctt
     1021 ccccgccggt gatggcaacc cgacactcct gccgctcctg aatctcgaaa tgtgggggct
     1081 gccaaccagc gagcccgcgc gggaccccaa caacggcaac ttcgtctacc agcggtttca
     1141 gcgcgggatc atgcagttcg acgcgacaac ggggacaact cagggattgc tgctgggcga
     1201 ctacctcaag gcgatcatga ccgggcaggg ccttccggcc gacatagcgg cccgggccgt
     1261 ctccaatccc ctgtacggcc agtacgatgc tgccaaccat cgcggtccgc tacatccgga
     1321 gttgctttca gcgaccaacc tcgacggtgc gttccagccc ggctggggcg atcctcggtg
     1381 tggggtggtc gtcgccggcc cctgcaccaa cgatcccaac tatgtggcca gatccctggc
     1441 atccctgggc gcgggcagtt ggtacgactt ctccaatggc agcgcttggc ccgatacggg
     1501 acgggcagag gtggttcgtc ccggggctaa gctgagcgct ctggcagatc gagccagagc
     1561 caaccctggg accgcctggc tggtcggcaa cgagcccaac gtgcccggcc aggacgacct
     1621 gaagccctcc gagtacgccg acttcctata tagggtggct gcggcaatca agggcgccga
     1681 ccccactgcc atcctggtgg ggcccaactc tctgaactgg gaagacacct gcacaagctg
     1741 cccgggcttc accaagggcc gcagttggtc cgaggagttc gtcacaaatt accggcagag
     1801 atacggtgcc ctgccgctgg acgtgtgggg gatccacgcc tatagccttg attgggagaa
     1861 cctgccgctc actcatgcca ccgccgtcga ggcgcagata gcaggagccg gggagtggga
//
LOCUS       DATALS010000300        10497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_255164, whole genome shotgun sequence.
ACCESSION   DATALS010000300 DATALS010000000
VERSION     DATALS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255164"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1061
                     /locus_tag="VHS06_06670"
     CDS             258..1061
                     /locus_tag="VHS06_06670"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX2987838.1"
                     /translation="MILAPREPAESVFRLETDQAIAQLTEFIQKEVKAAGRQRAVLGM
                     SGGIDSALVAHLATRALGPANVVGILLPFRTSSHESLLHAQMEIQKLGILHKTIEITP
                     IAEALFHQIPSMDKRRMGNAMARLRMVVLYDQSEENDALVIGTSNRTETLLGYGTLHG
                     DMAWGFNPIGCLYKTQVRQLAREIGVDPAIVGKTPTADLWAGQTDEGEIGVSYDVIDR
                     VLYLMVDRGMSRADVVALGYAPEAVDRVAGLVAGSEFKRRTPPVATVKP"
     gene            1200..2647
                     /gene="proS"
                     /locus_tag="VHS06_06675"
                     /pseudo
     CDS             1200..2647
                     /gene="proS"
                     /locus_tag="VHS06_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
     gene            2730..3662
                     /locus_tag="VHS06_06680"
     CDS             2730..3662
                     /locus_tag="VHS06_06680"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX2987839.1"
                     /translation="MPDFKDTIDALAAHQRMRRHLLQQLTDLSPEQLQQLKTVWSTLP
                     DPERINLLAYLRRQAEEDSLMDFDAVYEMAMEDPNSDVRRMAISAAVDAEDVSLMDRL
                     LEVVASDPDVMVRAAAAERLGPFACDAEVGKLPEEDGKRLETALLDKVKSETEDIRVR
                     AAALASAGYFSTDEVSAEIQRALTRSGLKTAALRAIGRNINPEEWTETLKESMGSDNP
                     DVRREAAAAAADYEDTVGDLGDLVDDPDRSVRLAAIASLGQIGGPEARDFLVYCYESE
                     DPEIRKAAAAAMDEIDSAESPLERFGPYDDTEEL"
     gene            4165..5019
                     /locus_tag="VHS06_06685"
     CDS             4165..5019
                     /locus_tag="VHS06_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2987840.1"
                     /translation="MDYRALAARTCTIVLWASAFAGIRAGLDGYNPGQLALLRFLFAS
                     AALAVYAALTHMRLPARRDILPLTIIGIVGLAGYHVALNYGEVSITAGAASLLIATTP
                     IMTAILAIVVLHEKVAPMAWIGIMISFAGAAMIALGEGGGITFDPAAVIVLGAAFTQA
                     AFFVAEKPYLAKYSALEVTSYTIWAATVALLFFLPGLPSAVVIAPLHATLAVAFLGIF
                     PAAVANVTWSYLLSRAPASNATVQLYLIPAVAILIAWGWLGEMPSALSLVGGAVALVG
                     VLLVTTRR"
     gene            complement(5162..6076)
                     /locus_tag="VHS06_06690"
     CDS             complement(5162..6076)
                     /locus_tag="VHS06_06690"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX2987841.1"
                     /translation="MPLLVTGGTGFVGSHLVRRLARQRSDIRLLVRSEAKAAAVRAMG
                     MEAVVGTVTDVDLLRRACDGVDSVVHLVAVIVERDGATFEAINVAGTANLIRVAESAG
                     VGRFVHLSALGAGEDPRFPYLYSKWRGAEIVRGSRMPYTILEPSILYGEGDDFLNRLA
                     GVARLSPVFLIAGSGATRFQPLWVEDLVTCLERCLDGKRHVGRTIALGGPEYLTYEQM
                     VDLLIRELGLRRWKIHMPLSLMRLAAGVMERTMSNPTVTPSQLDMLEIDNLTALDAVS
                     STFEFSPARLEEKIAYLNRPQAGSILGG"
     gene            6324..8198
                     /gene="ftsH"
                     /locus_tag="VHS06_06695"
     CDS             6324..8198
                     /gene="ftsH"
                     /locus_tag="VHS06_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX2987842.1"
                     /translation="MRFRNLQKNRRWIVLLAAVAILIVASTVLIFRSNSLSGKVDDQP
                     RPVNVDQVARLAKDGEIKTLSLNGDVVTARLTDGSSVISRKEAQVSILDTLRSYGVPD
                     SSLQQIGLEVRDPLAGNSQSGTWVLLLVSGVSVLLVLGVIYIGTKNGGAGGRYGSFTR
                     SGARVSKGASQNTRTAPSVTFRQVAGVDEAKQELEEVVEFLKSPQKFTALGATIPKGA
                     LLVGPPGTGKTLLAKAVAGEAGVPFFSISGSEFVEMYVGVGASRVRDLFSRARRSAPC
                     IVFIDEIDAVGRHRGSGMRHGHGGNDEREQTLNQILVEMDGFDGRSGVVVLAATNRPD
                     VLDEALLRPGRFDRQVTIDSPDIRGRKAILAIHARGKPISNEVSLDTLARQTPGFSGA
                     DLANLLNEAAILAARRGRKSIARADAEEAIDRCIAGPQRKSRVISEKERQITAYHELG
                     HALVATSLPNCDPVHKVTIIGRGRAGGFTQFLPTEDRSLWTRSQFIDTMASILGGHAA
                     EELVFGEVTTGASNDIEKATELAQRMVCNYGMSERFGPISLMKSDSSRSSSEPRMLSD
                     DLSRDIDREIHQLIDDARNRAKELLASRRETLESLTALLLERETLSGEEFRELAGTAK
                     "
     gene            complement(8359..9363)
                     /locus_tag="VHS06_06700"
     CDS             complement(8359..9363)
                     /locus_tag="VHS06_06700"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HEX2987843.1"
                     /translation="MEDRIDRLVNLDIGGRGAEGLYQAAREATGGALAMNAARGLVER
                     LKPGDPILVTTGFMARALVAPSIGETDGPPGTAVLVRALQKTLGVLPVFVTDELLVPA
                     MGSVLQAAGFSLLDLDEARQATTPGGRRTASAVLASFPAEDSEAAESADTLLEKLHPR
                     FAIAVERPGRNVAGYYHDMRGQGFQAGPARTDLLMERAQELGIPLLAVGDGGNEIGMG
                     LVRDAVMEHVPYGRSCRCPCGRGLAARTRADLLVTAAVSNWGCYAIVACLAILKEDAS
                     ILHTAKAEQRLLAASARAGLIDSVSGCVDDGVDGLPAETHMAVVQLLHTMAGQSIAEK
                     "
     gene            complement(9664..>10497)
                     /locus_tag="VHS06_06705"
     CDS             complement(9664..>10497)
                     /locus_tag="VHS06_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2987844.1"
                     /translation="KGEDYYLSFLPPSMQEMESPPEFIIQQMAHAGIDRVVLQNAHPY
                     GRLEEYFADAVSRYPDKFIPLAGVDETRASEADEIRRLRVSVKELGMRGLYYANRGLF
                     LEGHRRSFDDESFEPFWHEVETLGIPVFWEMGAVPEPTPPMLLREIDRLNRWAERHPT
                     VPCLYTHGLAPDLLEGNTPEPVGRLLENEQFMIELLYPIHWARDHQYPFPELRPVLRT
                     LYDAVGSERLAWGSDMPNVERNCTYKQSLQYIHPGFDFLPSSDIDRILGKNVLRLLKL
                     S"
BASE COUNT         2141 a         3260 c         3190 g         1906 t
ORIGIN      
        1 gcgcaagccg tggatcgcgt ggtctgcaag ggccacctat gccgtctcgt agggcggggg
       61 cttgtgcccg gcccagaacg cggtcggaat caccgtggtg gtcgtagcca cggtgtaaga
      121 ggcggggcac aagaccccgc cctacgtcca agcaacggcg aacccccttt tccccgcgta
      181 catagaagcg tacacggaac agacatgcga tcctacccaa ccacgtccca gattaatctc
      241 cttcggaggt gactaccgtg atcctcgcgc cccgggaacc ggctgaatcc gtcttcaggc
      301 tggagactga tcaggccatc gcccaactaa ccgagttcat acagaaggaa gtgaaggccg
      361 cggggaggca acgggcggtg ctgggaatgt ccggcggcat agactcggcc ctcgtcgccc
      421 atctggcaac ccgtgccctc ggccctgcca acgtcgtcgg gatcttgctt ccgttcagaa
      481 ccagcagcca cgagagtctc ctccacgccc aaatggagat ccaaaagttg ggtattctgc
      541 acaagaccat cgagattact cctatagcag aggcactgtt ccaccagatc ccctccatgg
      601 acaaacgccg catggggaac gccatggcca gattgcggat ggtcgtgctg tacgatcagt
      661 ctgaagagaa cgacgccctg gtcatcggta ccagcaacag gacggagacg ctgcttgggt
      721 acggcacgct ccatggagac atggcatggg gtttcaatcc catcggatgc ctctacaaga
      781 cgcaggtacg ccaactggca cgagagatcg gggtagatcc cgcgatagtc ggcaagacgc
      841 cgacagccga tctctgggcc ggacaaacgg atgagggcga gatcggcgtc tcctacgatg
      901 taatagaccg tgtgctatac ttgatggttg accggggtat gagccgcgcc gatgtggtag
      961 cgctgggata cgcccccgaa gccgtggacc gcgttgccgg attggtggcc ggctccgaat
     1021 tcaagcgccg tacgccgccg gtcgcaacag tgaagccgta ggtcaaagcg gccgcaacca
     1081 gttccaccga caatgtggtt taccgctgca tggaggcccc tggaggcctc cttttcgccc
     1141 ccggagcggg cacggtttga gcggttccac cgactcgcag accactgatg agaggaaaga
     1201 tgtccgaaca ggaatccttt gttgcagagc tgaccgacaa gagcgacgac tactcacgct
     1261 ggtacaccga ggtgatcctg aaggcgcagc tggcggatta cgccccggtg cgcggatgca
     1321 tggtcattcg cccctacggt tacgccctct gggagaacat gcagcggctc ttggatgccc
     1381 gcttcaaggc cactggccat actaacgctt acttcccgct cctgattccg gaaagcttcc
     1441 tcgcgcgcga ggccgagcac gtggccggat ttgccccaga agtggcctgg gttacccagg
     1501 gcggtaacga gaaactggag gaacggctcg ccattcgtcc cacctccgag gccatcatcg
     1561 gcatgatgta ctccaagtgg gtccagtcgt ggcgcgacct gccgatcctg atcaaccagt
     1621 ggtgcaacgt gctgcgctgg gagaaggtca cgcgactctt cctgcgcact accgagttcc
     1681 tctggcagga aggccacaca gtgcaccgga ccgaggaaga ggcacaggaa gagaccatga
     1741 agatcctcgg cgtctatcgc aactttgtcg aggaggatct ggccgtaccg gtaatcagcg
     1801 gacaaaagac ggaggccgag aaggtcaagg gcgccgagag gacctactcc atcgaggcga
     1861 tgatggcgac ggtaaagcgc tccaggccgg cacgtcccac aacctcggcc agcacttcgc
     1921 caaggtgttc gatatcacct tccaggacat agatggggag cgaaggcacg cgtggcagac
     1981 cagctgggga gtatccacca ggctgatcgg tgcgatcatc atggtccacg gtgacgattc
     2041 cggtctcatc atgcctccga aggtggcccc cactcaggca gtggtgatcc caatctggcg
     2101 caagcagcac gagagggaag aggtcgccgc tttcgtcgac aagttgaagg agacactggg
     2161 cgcggagatt cgcttccagg tagactggga cgacgagcat acccccggtt ggaagtacaa
     2221 cgagtgggag atgcggggcg tgccgctgcg gatagaggtc ggtccccgcg acgtcaagtc
     2281 caaccaggtg gtgatggtgc gaagggacaa ccgcgctaag gaattcgtgc ccatggatgc
     2341 cgtgcacgcg cgcaccatcg aactgctgga tcagatccag aaggacatgc tccagaaggc
     2401 tcgcgacttc atggcccaga gaacctttac ggtacacagc ttcgaggagt tcaaagctca
     2461 actcgagaag cagaggggct tcctgatggc ccactggtgc ggaagtcccg agtgcgaggc
     2521 gaagatcaaa gaggagaccg ccgccaccat ccgctgcatc ccgatggacg cgaaaatgga
     2581 ggagggcaag tgcgtctggt gtggcgcctc tagctccagg cgtgtggtct tcggcagagc
     2641 atactagctg aacaacagtc gtcactgcag gccgcgggtg tccgatccgc ggcctgtgtt
     2701 tcgacactgc atgggagaag gtaactcgga tgcccgactt caaggatact atcgacgccc
     2761 tggccgccca ccaacggatg cggcgccatc tgctacaaca actgacagac ctctcgcccg
     2821 agcagttgca gcagttgaag acggtgtggt ccacgctccc cgacccagag aggatcaacc
     2881 tgctggccta cctccgtaga caggccgaag aggattccct gatggatttc gacgccgtct
     2941 acgagatggc gatggaagat ccgaacagcg acgttcgccg catggccatc tcggcagcgg
     3001 tagatgccga ggatgtgtcg ctgatggatc ggctgctcga agtcgttgcc tccgaccccg
     3061 acgtcatggt aagagccgcc gccgccgaaa gactagggcc gttcgcctgt gatgccgagg
     3121 tggggaaact cccggaggaa gatggcaagc ggctcgagac agcgctgctg gacaaagtga
     3181 aatcagagac cgaagacatt cgcgttcgtg ccgcagcact ggcgtccgcc ggctatttct
     3241 ctaccgacga ggtctctgcc gagatacagc gagcgctgac tcgatccggg ctgaagaccg
     3301 cggcgctgcg agccattggc cgaaacataa accctgaaga gtggaccgag actctcaagg
     3361 agtctatggg cagcgacaac cctgatgtga ggcgcgaagc cgcggcggca gcggcagact
     3421 acgaggatac ggtcggcgat ctcggcgacc tcgtggacga ccccgatcgt tcggtgcggc
     3481 tggcagccat cgcatcactg ggccagattg ggggccccga ggctagagat tttctcgtct
     3541 actgctatga gagtgaagat ccggaaattc ggaaggccgc agctgcagcg atggacgaga
     3601 tcgactcggc agaatcgccg cttgagcgct tcggcccgta cgatgacact gaggaactct
     3661 gaggaactga ggggaactcg ggggaactct aggaattcag ggagacagca cctcgaccac
     3721 tcggctagaa gaccctctga gtccctcgga gttccaagag ttcttctaac ttccttggct
     3781 cttagttgag cggcggcggc ttcaggttgc taccggcttg aggccacctg tggaggctga
     3841 actgcccaca ccagtctccg ccgtaagtct ctggccacac cccccgtcta tcaggagccc
     3901 gggacggcgc tgtggatgga acatacacgg gcgggttcac cctgcactcc ccgaccgtgt
     3961 cgtccttggg ctgctggcgt ccagccaatc gcgaatgcca gtataggcag taacggcact
     4021 ttctctccgc catagtagcg aacctcgggg tctaagactg tggccagggc atatcgacgt
     4081 cgcccatttg ggatcggttg cccggaagac cctgcccgat tatagcgcct ttccagtagt
     4141 cacaggaaat ggacaactgc cgccatggat tatcgtgccc tcgcagcacg cacgtgcaca
     4201 atcgtcctct gggcatcggc cttcgccgga atccgggctg gcctcgacgg ctacaacccg
     4261 gggcaactgg cgttgctgcg gtttctcttc gcctccgcag ccttggccgt ctatgccgcg
     4321 ctgacccaca tgcggctccc ggccaggcgt gacatcctgc ccctgacgat catcggcata
     4381 gtcgggctag caggttacca cgtggcattg aactacggtg aggtgagcat cactgccggg
     4441 gcagcaagcc tgctgatcgc gactaccccc atcatgacgg ccatcctggc catcgtcgtg
     4501 ctccacgaga aggtcgcgcc catggcatgg attggcatta tgatcagctt cgccggggcc
     4561 gcgatgattg cccttgggga gggaggtggg atcaccttcg atccggcagc ggtgattgtg
     4621 ctgggcgctg ccttcaccca ggccgccttc ttcgtcgctg agaagcccta cctggccaag
     4681 tactcagcgc tggaagtcac ttcctacacc atctgggcag ccactgttgc cctgctcttc
     4741 ttccttccgg gactcccatc ggccgtagtc attgccccgc tccacgcaac ccttgccgtc
     4801 gcattcctgg gcatctttcc agcggctgtc gccaacgtca cgtggagcta ccttctatcc
     4861 cgtgcaccgg cctccaatgc cacggttcag ctctacctga tcccggcagt cgcaattctg
     4921 atcgcatggg gttggctcgg ggagatgccc agcgccctct ccttggtcgg cggggccgtc
     4981 gccctggtag gcgtgctgct cgtgaccacc cgccggtagc aattcgaccc catgatcctc
     5041 acagtagggc gggtcgctcc cgcaacgcga gcgagcgcag cgttgcgccg cccgtcctca
     5101 tcccgtggct cgtggctatc ggccggtagt gatcagacgc cgtgatctcc tacaaccaag
     5161 actatcctcc aaggatggag ccggcctggg gccgattgag ataagcgatc ttctcttcca
     5221 gccttgccgg agagaactcg aatgtgctac tgacggcatc cagagccgtc aggttgtcta
     5281 tctccagcat gtccagctga gatggcgtca ctgtggggtt ggacatcgtc cgctccatga
     5341 ccccggcagc cagcctcatc agggaaaggg gcatgtggat cttccagcgg cgcagtccca
     5401 actcccgaat caacaggtcc accatctgct cgtaggtcag gtactccggt ccgccaagcg
     5461 caatcgtcct gcccacatgg cgcttgccat ccagacagcg ctccaggcag gtgaccagat
     5521 cttccaccca gagcggctga aagcgggtgg cgcccgagcc ggctatgagg aagacgggcg
     5581 agagtctagc cacccctgcc aggcggttca ggaagtcgtc accttcccca tacaggatcg
     5641 acggctccag gatcgtgtag ggcatgcggc tcccacgtac gatctccgcg cccctccact
     5701 tggaatagag gtatgggaag cgtgggtcct cgcctgcacc cagcgccgat aggtgcacga
     5761 agcggccaac ccctgccgac tcggccaccc tgatcagatt ggccgtgcct gcgacgttga
     5821 tcgcttcgaa ggtcgctccg tccctctcaa cgattaccgc caccaggtgt accaccgagt
     5881 ccacgccatc gcatgcacgg cgaagcaaat cgacgtccgt aacggtcccg accacggcct
     5941 ccatgcccat agcccgaacc gcagccgcct tggcctccga gcgaaccagc aaccgtatat
     6001 ccgaacgctg tcgggccagc ctcctgacga ggtgagagcc gacgaaccct gtgccgcccg
     6061 tgaccagcag aggcatctca taccccccaa cgcactgctt cttctcccat cctgagtgct
     6121 gtagatccta tcatcatttg tcgtgcgtgt gccatagtgt ataattcctt tacgcacttg
     6181 ctcacaggga atcaggtttc actccattcc cactgtcacc aggtgatctc tcttcggcat
     6241 cagtcgatcc gctatggaca cccgtgtgac cgagcagttg tgttcgatct ccctcaagtc
     6301 cgtaccagaa cgtgaggcta gtgatgcgtt tccgcaattt gcagaagaac cgacgatgga
     6361 tagtgttgct cgctgccgta gccatcttga ttgtggccag cacggtactg atctttagga
     6421 gcaacagcct gtcgggcaag gttgacgatc agccccgacc ggtcaacgtg gaccaagttg
     6481 cccgactggc caaggacggg gaaatcaaga cgctctctct gaacggagat gttgtcactg
     6541 ctaggctaac cgatggctcc agcgtcattt cccgcaagga agcccaggta tcgatcctgg
     6601 acaccctgcg cagctacggt gttccggaca gcagccttca gcagatcggt ctagaggtca
     6661 gggatccgct ggcaggcaac agccagagtg ggacctgggt cttgctcctg gtctccggtg
     6721 tttccgtgct cctggtgctg ggtgtcatct atatcggcac caagaatggt ggagccggcg
     6781 gcaggtacgg atccttcaca agaagtgggg cgcgtgtttc gaagggcgct tcccagaaca
     6841 ccagaacggc cccatcggtc acattccggc aggtcgctgg agttgacgag gcaaagcagg
     6901 aactggaaga ggtggtggag ttcctcaaga gccctcagaa gttcactgcg ctgggcgcga
     6961 ccatccccaa gggagccctg ctggttggtc ctccaggcac gggcaagacg ttgctcgcaa
     7021 aggccgtggc cggcgaggca ggcgtaccct tcttcagcat cagtggatct gagtttgtgg
     7081 agatgtacgt gggcgtaggc gcgtccaggg tgagagatct cttctccaga gcgagaaggt
     7141 ctgccccctg catcgtcttc atcgacgaga tcgacgccgt cggccgccac cgcggctccg
     7201 ggatgcgcca tgggcacggc ggcaacgacg agcgagagca gactcttaac cagatcctgg
     7261 tcgagatgga cggcttcgat ggccgctccg gtgtcgtggt gctggctgcc accaaccggc
     7321 cggacgtgct ggatgaggcg ctgttgcgcc caggcaggtt cgaccgacaa gtgacgatcg
     7381 actccccgga catcagggga cgaaaggcca tactcgccat ccacgcccgc gggaagccga
     7441 tctccaatga ggtgagcctc gacacgctgg cgaggcagac tccaggcttc tctggggcag
     7501 atctcgccaa tctgttgaac gaggcggcca tcctggccgc aaggcgaggc aggaagtcca
     7561 tcgccagggc agacgccgag gaggctatcg accgctgcat cgccggacct cagcgcaaga
     7621 gcagggtgat cagcgagaag gagcggcaga taaccgctta ccatgaactg ggccatgcgt
     7681 tggtcgcgac ctcgctgccc aactgcgacc cggtccacaa ggtcacaatc ataggcagag
     7741 gtagggcggg cggctttacg cagttccttc ccaccgagga tcggtcactc tggactcgat
     7801 cgcagttcat agacacgatg gcctcgatcc tggggggcca cgcggccgag gaattggtgt
     7861 tcggggaggt cacaactggg gcctccaacg acatcgagaa ggcgacggaa ctcgcacagc
     7921 gcatggtgtg caactatggg atgagcgagc gcttcggccc catctcgctg atgaagtcgg
     7981 actccagcag gagttcgtcg gagcctagaa tgctgagcga cgatctatcg cgcgacatcg
     8041 acagggagat tcatcaactg atcgacgacg cacgcaatag ggcgaaggag ttgctggcat
     8101 cccggcgaga gacattagag tcgctgaccg ccctgctgct ggagcgcgag acgctgagcg
     8161 gcgaggagtt cagggagctg gccggcaccg caaagtagcg ccccgcacca ctagaggtac
     8221 acggttctgt catgctgagc gaagcgaagc atctcctcgt agacggagag atccttcgct
     8281 gccgctcagg acgacagctg taaggatcag tgcggtgggg aacgcctaga acgagtaggg
     8341 gcgacgcatg cgtcgcccct atttttctgc aatcgactgg ccggccattg tgtgcagcaa
     8401 ctgcacgacc gccatgtggg tctctgcagg caacccatct accccgtcgt cgacacatcc
     8461 gctcaccgag tctatcaatc cagccctggc gctggccgcc agcaaccgct gctcggcttt
     8521 cgcagtgtgg aggatcgagg catcctcctt gaggatggcc agacacgcga cgatagcata
     8581 gcagccccag ttggagaccg ccgccgtcac caaaagatct gctctggtcc gagccgcaag
     8641 cccgcgtccg catgggcagc ggcaggaacg gccgtagggc acgtgctcca ttaccgcatc
     8701 ccgcaccaac cccatgccga tctcgtttcc accatccccc accgccagga gcgggatgcc
     8761 caactcctga gcccgttcca tcagcaggtc cgtgcgtgca gggccggcct gaaagccctg
     8821 tccgcgcata tcgtgatagt agccggcaac gtttctcccc ggcctttcca ccgcgatggc
     8881 gaagcgaggg tgaagcttct ccagcagcgt gtctgccgat tccgcggctt cggaatcctc
     8941 ggcagggaag gaagccagca ccgcagacgc ggtcctgcgt ccgccgggag tggtagcctg
     9001 ccgcgcttca tccagatcca gaaggctgaa tcccgcagcc tgcaggacgc tccccattgc
     9061 agggacgagc agttcgtccg tgacgaacac gggaagtact cccagggtct tctggagcgc
     9121 cctcacaagt actgcggtac cgggaggccc gtcggtctcc ccaatggaag gcgccaccaa
     9181 agcccttgcc atgaagccgg tagtgaccag aatggggtca cccggcttca gccgctctac
     9241 taagcccctg gccgcgttca tggccagcgc tccccccgtt gcctccctcg ccgcctgata
     9301 gagcccctcg gcgcccctac ccccgatgtc caggttaacc agccgatcga tacgatcctc
     9361 catgatcgtc acacaaacct ccccgagtta ggcaagcgcc agggatatgt cggagactcc
     9421 acgcctgggt gcacgagcgc acaacgcaac tcgcggccaa attgagtgcg agcaagagtg
     9481 gggcaacaca ggcgtgcaca ggcataatac cagttttccg aagtgaaggc ttccacagtc
     9541 gatccggcgt ttgggccgat cggtgtgatc cacagagtgt agcgattggg ggctcgcgac
     9601 tccggcacaa cttctgctaa ctacaaccgt gccggagccg ccgcacaagc ctcgataccc
     9661 atactaggac agcttcagca gccgcagaac attcttgcca aggatgcgat cgatatcgga
     9721 cgaaggcagg aagtcgaagc caggatgaat gtactgcaaa gactgcttgt aggtgcagtt
     9781 ccgctccaca ttgggcatgt cggatcccca ggcaagccgt tcggatccca cggcgtcata
     9841 cagtgtgcgc agaacgggcc gtagttcggg gaaggggtac tggtgatcgc gcgcccagtg
     9901 tatggggtac agaagctcaa tcatgaactg ctcgttttcc agcagacggc ctaccggctc
     9961 gggagtgttt ccttccagta gatccggggc aagtccatga gtgtaaagac agggaacagt
    10021 gggatggcgt tcggcccatc ggttcagccg atcgatctcc cgaagcaaca tcggcggggt
    10081 gggctcgggc acggctccca tctcccagaa gacggggatg cccagggtct ccacctcgtg
    10141 ccagaagggt tcgaaggact cgtcgtcgaa ggatctgcga tggccctcca ggaagagtcc
    10201 ccggttcgcg tagtagagtc cgcgcatccc caactccttg acggatacgc gcaaccgccg
    10261 aatctcgtcg gcctcggagg cccgagtctc gtccacacct gccaggggaa tgaacttgtc
    10321 tggatagcgg ctcacggcgt cggcgaaata ctcctccagc cgcccgtagg ggtgggcatt
    10381 ctggaggaca acccggtcga tgcctgcatg cgccatttgc tggatgatga actcgggcgg
    10441 gctctccatc tcctgcatac tgggtggcag aaagctcagg tagtaatcct ctccttt
//
LOCUS       DATALS010000301         5814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_165724, whole genome shotgun sequence.
ACCESSION   DATALS010000301 DATALS010000000
VERSION     DATALS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165724"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /locus_tag="VHS06_06710"
     CDS             <1..1617
                     /locus_tag="VHS06_06710"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HEX2987845.1"
                     /translation="VRLPEGTNPALGELTVETSPSLAAGMRYSARFLDEFPYECIEQT
                     VSRFLPRVVMQSAFDKLKLSDREGIAGRLPSIVQRSLQKLYSGQSRDGGWGWWPGESG
                     DQWITAYAVQGLSEARRAGYTVDDSVLDRAASFLRKSLDTRYDAEHPENANSRAYVLY
                     ALALAGKGDVGLTNSLYDRRATLGNYGRAYLALAFQTLGASDQDGKVKSLISDLSSAA
                     ISSAAGSHWEEATIDYRAMNSNTRSTAVVLDALVRLAPDNPLIQPAVRWLMVARKEGH
                     WETTQETAASLLALTDYLSASGELKGDFAYRVSLNGKDLATQTVTPSNVDEAKRLVVE
                     LKELVGGGDSLVNIARANPSGGQSGDGKLYYTMALRYFLPGDKVPAVAEGIGLIREYY
                     RLGDEASGPVTQVAPGETVKVKLTMVALRDLHYFAVEDPLPSGLEAIDTRLKTSSVSA
                     RGETGMVDKSGKEGAVLPWWKYDYFTQVEPRDDRVTLFATYLPKGTYEYTYLAKATSQ
                     GEFQALPARGYEMYFPEVWGRSEGSKFTVR"
     gene            complement(1704..2786)
                     /locus_tag="VHS06_06715"
     CDS             complement(1704..2786)
                     /locus_tag="VHS06_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2987846.1"
                     /translation="MANRKEQHVDLDVLISKGKEQGYLRLHEIEEAVGSADPDVVTAV
                     DDAIAAEGIEVVEDEDEEEAVQGDNALDHDLESMESSGQVASEDPLLSYMRDIADVPL
                     LTAAQEVELAKRIEQGDAEALEKFIMSNLRLVVSVAKRYTGRGLPLLDLIQDGNIGLM
                     RAVQKYDWRKGFKFSTYATWWIRQAITRAIADKARTIRLPVHMGEAITRLNQVSQRLT
                     QQLGREPTEDELAEALGIGVQKVRDAIKAAHVPSSLEAPIGEEEESELAEMVADEISQ
                     HPEDAVYEMLLKRETQDALGSVLTPREQLVLQLRFGLGNGHVYPLEKIGEVLGITRER
                     VRQIEAKALEKLRHPSVAKSLRSYLE"
     gene            complement(3003..4100)
                     /locus_tag="VHS06_06720"
     CDS             complement(3003..4100)
                     /locus_tag="VHS06_06720"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX2987847.1"
                     /translation="MRDKSIGLVLVTGGAGFIGCNLAAHYLSKGWSVRLFDNLSRPGT
                     EKNLSWLKSIGDGRLEVAIADVRDDGELRRAASDADVIFHMASQVGVTTSVSDPRYDF
                     EVNALGTLNMLEAARASGRFPVVVYASTNKVYGSMPTAGVELTDGGYRYSKLPMGVSE
                     EWPLDFHSPYGCSKGAADQYVRDYARIYGLPTVVFRQSCVYGPHQFGNEDQGWVCHFA
                     ISALRGLPITLYGDGHQVRDVLHVDDLVAAYELAVANSEISRGRVYNIGGGLTNAISL
                     QGLLQLLQAEVGYPLSVSFGEWRPGDQRVYISDMRKAKGELGWEPRVGARRGIAGLLA
                     WLRENWSLFSRIDGVAMVDLATLTRERLPVG"
     gene            complement(4313..4921)
                     /gene="gmhB"
                     /locus_tag="VHS06_06725"
     CDS             complement(4313..4921)
                     /gene="gmhB"
                     /locus_tag="VHS06_06725"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEX2987848.1"
                     /translation="MSRVVFIDRDGVINRNLGSHVKRWEEFVFLPGAIEALAALTRHG
                     FQSIVLTNQAAINRGIITRETLEGIHTRMLGRIEAVGARIDGVFYCPHRPDEHCSCRK
                     PQPGLLYQAAERFGVKLSESYLVGDAITDIMAAKTAGCWPILVMTGRGIPAYLSPTAR
                     RIEGYSVARNLQHAVHRILLEEGLVEPSFLDRVWRRPLRQPR"
     gene            complement(4939..>5814)
                     /locus_tag="VHS06_06730"
     CDS             complement(4939..>5814)
                     /locus_tag="VHS06_06730"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2987849.1"
                     /translation="GTVLISVWFKVVKWHMLLLPTGNLSKSNLFYAINVGYLVSTVLP
                     GRLGELARAYMLARVERVSPVAVLSTVALDRVLDVVAMALLLAVVLPTTDLPPQIGQS
                     GFLIGAGGVAVLAVCMALAYPAGRELFLRLLRGLPKFPGKPLAEHFAEALCDGMSGLR
                     GAGAVMKIALATLGVWLTTVVTAYGSMQAFHISAPIWAAALVVAITNLGMAVPSTPGY
                     FGVSEYLTVLALSAFGVDKESALGCALIYHLAWIVPVNLLGVFAMWRFGMSLMGWQKL
                     DPEDQGDPAAVGSGK"
BASE COUNT         1122 a         1900 c         1715 g         1077 t
ORIGIN      
        1 gtccggctgc cggaggggac gaatccggcg ctgggggagt tgacggtgga gacctcccca
       61 tctctggcgg cagggatgcg ctactcggcg cgcttcctgg atgagtttcc ctacgagtgt
      121 atcgagcaga cggtgagccg cttccttccc agggttgtga tgcaaagtgc gttcgacaag
      181 ctcaagcttt ccgaccgcga ggggatcgcc ggcagactcc ccagtatcgt tcagcgcagc
      241 ctgcagaagt tgtactcggg acaatcccgg gacggcggct ggggctggtg gccgggagag
      301 agcggcgacc agtggattac cgcctacgcg gtgcaggggc tgtctgaggc tcgccgtgcg
      361 ggctacacgg tggacgactc cgtgctagat cgtgcggcct cgttcctcag gaagtcgctg
      421 gataccaggt acgacgcgga gcaccctgag aacgccaact ctcgggccta tgtactctac
      481 gccctggccc tggccgggaa gggggatgtg ggcctgacca attccctcta cgatcgcagg
      541 gcgacgctgg gcaactacgg gcgagcctat ctggcgctgg ccttccagac gctgggcgcg
      601 tcggaccagg atggcaaggt caagagcctg atctccgacc tctcctcggc cgcgatcagc
      661 agcgcggccg gcagccattg ggaggaggcg actatcgact atcgagccat gaacagcaat
      721 acccgcagca ctgcagtggt gctggatgcg ctggtgcggc tcgcgccgga caatccgctg
      781 atccagccgg ccgttcgatg gttgatggtg gcccgcaagg aaggccactg ggagacaacc
      841 caggagactg ccgcaagcct gctggcgctc acggactacc tctcggccag cggcgagttg
      901 aagggcgact tcgcgtatcg ggtcagcctc aacgggaagg atcttgccac ccagaccgta
      961 acgccgtcca acgtggacga ggcgaagcgg ctcgtcgtgg agttgaagga gttggtgggt
     1021 ggaggcgata gcctggtgaa catcgcccgc gcgaaccctt ctggcgggca gagcggcgac
     1081 ggcaaactgt actacaccat ggcgctgcgc tacttcctcc ctggcgacaa ggtgccggcc
     1141 gtggcagagg ggatcggcct gatccgggag tactaccggc tgggcgacga ggcctccgga
     1201 ccggttactc aggtggcccc cggtgagacg gtgaaggtga agctgaccat ggtggcgctg
     1261 cgagacctgc actactttgc ggtggaggat cctctgcctt ccggtctgga ggcgatagac
     1321 actcgcctga agaccagcag cgtatcggcc cgcggcgaga cagggatggt ggacaagagt
     1381 ggtaaggagg gggcggttct tccctggtgg aagtacgatt actttacaca ggtggagccg
     1441 cgggatgatc gggtcaccct ctttgccacc tatttgccga aggggaccta cgaatacacg
     1501 tatcttgcaa aggccaccag ccagggagag ttccaggctt tgcctgccag gggttacgag
     1561 atgtacttcc ccgaggtgtg gggacgcagc gaggggagca agttcacagt gaggtaggtc
     1621 tcgctgtcgt agggcggggc acaagacccc gccctacaca ctcgggcacc gcgtccgcaa
     1681 cctcgacact accatcgccg cggctactcc aggtagctcc tcagcgattt cgccacagag
     1741 gggtggcgaa gcttctccaa cgccttcgcc tctatctgcc gcacccgctc ccgcgtgatc
     1801 ccaagcacct cgccgatctt ctccagtgga tacacgtgcc cgtttccgag cccgaagcgt
     1861 agctgaagca ccaactgctc tcggggcgtc agcaccgagc ccaacgcgtc ctgagtctct
     1921 cgcttcaaca gcatttcgta gacggcatcc tcgggatgct gagaaatctc gtcggcaacc
     1981 atctctgcca actccgactc ctcctcctcc ccaataggag cctccagcga ggatggcacg
     2041 tgagccgctt tgatcgcgtc ccgcaccttc tgcacaccga tccccaacgc ctcagccagt
     2101 tcgtcctcgg tgggctccct gccaagctgc tgggtgagcc gctgggatac ctggttcagc
     2161 cgggtgatcg cctcacccat gtgaacgggc agtctaatgg tcctcgcctt gtcggcgatg
     2221 gcgcgggtga tcgcctgcct gatccaccag gtcgcgtagg tggaaaactt gaaccccttg
     2281 cgccaatcgt acttctggac ggctcgcatc aacccgatgt tgccgtcctg gatcagatct
     2341 agaagcggaa gacctctgcc cgtgtaccgc ttcgcgacgc tgaccacaag tcgcaggttg
     2401 gacatgatga acttttcgag cgcctctgcg tccccctgct cgatcctctt cgccagttcc
     2461 acctcctggg cagcggtcaa cagcggcacg tcggcaatgt cccgcatgta ggagagcagc
     2521 gggtcctcgc ttgccacctg gcccgaagac tccatagatt cgagatcgtg gtcgagggca
     2581 ttgtcccctt gaacagcctc ttcctcgtcc tcgtcttcta ccacctcgat gccctctgcc
     2641 gcgatggcat cgtccactgc agtcacgaca tccggatccg cactgccgac ggcttcctct
     2701 atctcgtgca atctgagata gccctgctct ttccccttac tgatcaggac gtccaagtca
     2761 acatgttgct cttttcgatt tgccattggg taccccccgc cggcgggcaa caagtattgc
     2821 cgctcgccct tccgggctcg cggcacgctg ttcctcgtcc ccgccgtctc tgggacgtcg
     2881 acggcgacgc ttcctagagg ctagttcggg gacggcaaga tgcgtgcccc ccgaccatgc
     2941 atttagctgg tcagggggca cttgctcgcc gcaattctca tcgaaacgta gctggatcgc
     3001 ttctagccta ccggcagcct ctccctagtg agcgtcgcca aatccaccat cgcgacaccg
     3061 tctatcctgc tgaagaggct ccagttctcc ctgagccatg ccaggagccc cgcaattcct
     3121 ctcctcgcac ccaccctcgg ctcccagcca agctcgccct tcgccttccg catgtcactt
     3181 atgtaaacac gctggtcgcc cggtctccac tcgccgaagg atacgctcaa cgggtatccc
     3241 acctcagcct gaagcaactg cagcaggccc tgcaaggaga tcgcgttggt caaaccaccg
     3301 ccgatgttgt acaccctgcc ccgggagatc tcgctgttcg ccaccgcaag ctcgtaggca
     3361 gccaccagat cgtctacgtg cagcacatcc ctgacctgat gcccatcccc gtacagcgtt
     3421 attggcaacc cacgaagcgc cgatatggca aagtggcaca cccagccctg gtcctcattg
     3481 ccaaactggt gcgggccgta aacacacgac tgtctgaaga ccactgtcgg caacccatag
     3541 atgcgggcgt agtctcgcac gtactgatcg gcagccccct tggaacagcc gtacggcgag
     3601 tggaagtcca gtggccactc ctcggaaacc cccataggaa gcttgctgta gcgatagccc
     3661 ccatccgtca actccacgcc cgccgtaggc atgcttccat agaccttgtt ggtcgaggcg
     3721 tataccacca ccgggaaccg gccagaagcc ctcgcggcct ccagcatatt gagagttccc
     3781 agggcgttca cctcgaaatc gtagcgaggg tccgacaccg aggtcgttac gcccacctgg
     3841 gaagccatat gaaagatcac atctgcgtca gaggcggctc ggcgcagttc accatcatcc
     3901 ctgacatcgg cgatggcaac ctcgagccgt ccgtccccaa tcgacttcag ccagctgagg
     3961 ttcttctcgg tgcccggtcg agacaggtta tcgaacaggc gaacggacca gcccttggag
     4021 aggtagtggg cggcgaggtt gcacccgatg aatccggcac ccccggtcac caggaccagg
     4081 ccaatactct tgtcccgcaa tgactcttct cccccttgat cgaagctggt gcctatccgt
     4141 agagcagtct actgctattc gccgaggaaa gcactcgcag gctgttgcca cgcagcataa
     4201 tgccagtacc tgcttctttc acgtgtatca agatcagtac caggaaagta catcctcgcc
     4261 ggcggaagga tgcgtggggc tgttcatcgg ccgaggctga tcccctggcc ggctatcgtg
     4321 gctgtctgag gggccggcgc cagactcggt cgaggaaact cggctccaca agcccctctt
     4381 ccaaaaggat cctgtggaca gcatgttgca agttcctggc cacggagtag ccctcgatcc
     4441 gcctggcagt aggagagagg tacgctggaa taccgcgccc ggtcatgacc agaattggcc
     4501 agcatcccgc ggtctttgct gccattatgt cggtgatggc atcgccgacc aggtaggact
     4561 cggacagctt caccccaaaa cgctcggccg cctggtacag cagccccggc tgtggctttc
     4621 tgcaggagca gtgctcatct gggcggtgag ggcagtagaa tacgccgtcg atccgcgctc
     4681 cgaccgcctc gatccgcccc agcatccggg tgtggatccc ctccagagtc tctcgcgtga
     4741 taatcccgcg attgatggcg gcctggttgg tcaagacgat gctttgaaag ccatgcctgg
     4801 tcaacgccgc cagcgcctcg atagcccccg gcaggaagac aaactcttcc cagcgcttta
     4861 cgtggctgcc aaggtttctg ttgatcacac catcgcgatc gatgaagaca actctcgaca
     4921 tgtcggcccc tgagggtgct acttaccgct acccactgcg gcgggatcgc cctggtcttc
     4981 aggatccagc ttctgccatc ccatgaggga catgccgaag cgccacatgg caaacacccc
     5041 cagcaggttg accggaacga tccaggccag gtgatagatc agcgcgcagc ctagagccga
     5101 ctccttgtct acgccgaaag cgctcagggc aagcaccgtg aggtactccg agacgccgaa
     5161 gtacccgggg gtggagggca cagccatgcc gagattggtg atggcaacca ccaacgcggc
     5221 tgcccagatt ggggcggaga tgtggaatgc ctgcatggat ccgtatgcgg tcacgaccgt
     5281 ggtcagccaa acgcctaagg tggccagcgc gatcttcatc acggccccgg cgccccgcag
     5341 cccggacatg ccgtcacaca gcgcttcagc aaaatgctcc gccagcggct tgcccgggaa
     5401 ctttggcaga ccgcgcaata gtctcagaaa caactctctc cctgctggat aggcgagggc
     5461 catgcagacg gcaaggacgg ctacccctcc cgcgccgatc aggaaacccg actggccgat
     5521 ctggggcggc agatcggtgg tggggagcac aacggccagt aacaacgcca tggcaacgac
     5581 gtccagcacc ctgtccagcg ccaccgtgga gagcaccgcc acggggctta cccgctcgac
     5641 cctcgcaagc atgtacgcgc gggccagttc acccagtctg ccgggaagca ctgtgctaac
     5701 cagatagccg acgttgatcg cgtaaaacag gttgctcttg gagaggttgc cggtggggag
     5761 cagcaacatg tgccacttga ccaccttaaa ccacaccgag atcagcaccg tgcc
//
LOCUS       DATALS010000302         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_284837, whole genome shotgun sequence.
ACCESSION   DATALS010000302 DATALS010000000
VERSION     DATALS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284837"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            473..1804
                     /locus_tag="VHS06_06735"
     CDS             473..1804
                     /locus_tag="VHS06_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005485011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HEX2987850.1"
                     /translation="MDPDTPQPASSMAGVPVYPDVKLYANILDNMRIWEGDGWKPETM
                     SWKTGCYLHSGLSGFTEFTFRGPEAARFLSMLSINGSDWPIGTSKHLVMLDEEGLIAN
                     HTLGVRDSEDSFRHFACPPWSIYKYQTAGMNVELGVREIFILQVAGPTSLQVLERATG
                     ESLRDVGFLDVRPTRVPDVDTAIEVTRIGMAGTLAYELRGDFSAGPAVYDRVYRAGHD
                     LGIKRLGWRSYMVNHVEGGFPQVLGTFMYSGARDPQFVKMLPPTVANTEWTGSVDPSD
                     LRARFRTPVELGWEWMARFDHDFIGREALEKEMAHPKRTIVTLRWNRDDVIDIYASLF
                     HPGEEYRTLDLPSSPQPPAGGHADRVTKGGQQIGVSSGTTYSYYFREVISHCTIDRDQ
                     AEIGNEVIVHWGDYGKRIKEVRATVERFPYLDLPRNQTYDLRTVPSGVPAV"
BASE COUNT          385 a          537 c          569 g          352 t
ORIGIN      
        1 aacaacgaag caacaagtgc attgagcaag gcattattag acggacttgc agtcgctgtc
       61 aagttgccga cacagctaac tatgccgccc gttcatcgaa cgcatccgga cgagagcagg
      121 ctattgggcc gggccgggta gaaccagcga taggggtcgt ctcgacggcg ggcgcgcctt
      181 ggaactccga tcaagcgagc tgggagatgg aaacgacatc aacatgatgc ttatgaggac
      241 taacttcgag caccggtgac ctccaatagc ttgtcgccga agccttgaca cgaatgctcg
      301 tctggcgtat atggttcacg caacgacagc tcgtgtatgt tccttgagcc acgaaatggc
      361 atggccctcc gagcacacgc gccggcaagg aacggtggtc ggcatcatcc gttcggagtg
      421 tgattccact tcggcgagag gcgacgtcca gcatttagga ggtcaacaca ttatggatcc
      481 cgacacgccg caaccagcat cgtccatggc aggggtgccc gtctatcccg acgttaagtt
      541 gtacgccaac atactggaca acatgcgcat ttgggagggc gatggctgga agccggagac
      601 catgtcctgg aagacaggct gctacctcca ctcggggctc tccggcttca ccgagttcac
      661 tttcagaggt ccggaggctg ctcggtttct ctcgatgctc agcattaatg ggtcggactg
      721 gccgattggt acgtcgaaac acctagtgat gctggacgag gaggggctga tcgccaacca
      781 cacgctgggg gtgcgggaca gcgaggatag cttccgccac ttcgcctgcc ctccttggtc
      841 gatctacaag taccagacgg ccggtatgaa tgtggaactg ggcgtccggg agatcttcat
      901 cctccaagta gccggaccca cctcgctgca ggtgctcgaa cgcgcgaccg gcgaaagcct
      961 cagggacgtc gggttccttg atgtccggcc gactcgtgtg ccggacgtcg acacggcaat
     1021 cgaggtgacc cgcatcggaa tggccggcac gctcgcctat gagctacgcg gtgacttctc
     1081 tgccgggccc gcggtatacg acagggtcta ccgtgccggg cacgacttag gcatcaagcg
     1141 tctgggctgg cggagctaca tggtcaacca cgtcgagggc gggtttcctc aggttcttgg
     1201 cacattcatg tactcaggcg cgagggatcc tcagttcgtc aagatgttgc ctccgacggt
     1261 cgcgaatacg gagtggacgg gtagtgtgga cccgtctgat ctccgggccc gtttccgcac
     1321 tccggtcgag ctgggctggg aatggatggc ccgcttcgac cacgacttca tcgggcggga
     1381 agcgctcgag aaggagatgg cccatcccaa gcgaacaatc gtgactctac gctggaatcg
     1441 ggacgacgtc atcgacatat atgcctcgct cttccatccg ggcgaggagt atcggacgct
     1501 tgacctaccg agtagtcctc agccgccggc cggcgggcac gcggatcgtg tcacaaaggg
     1561 cggtcagcag atcggggtct cctcagggac aacctatagc tactacttcc gcgaagtcat
     1621 ctcccactgc accatcgacc gcgaccaagc tgagatcggt aatgaggtca tcgttcattg
     1681 gggcgactac gggaagcgca tcaaggaagt gcgggccacg gtcgagcgtt tcccctatct
     1741 cgatctgcca cgcaaccaga cctacgatct gaggaccgtc ccgtcgggcg ttccggcggt
     1801 ttgagccgga gatcgcgagc aaagcaggac ggagatgata acc
//
LOCUS       DATALS010000303         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_59810,
            whole genome shotgun sequence.
ACCESSION   DATALS010000303 DATALS010000000
VERSION     DATALS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59810"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VHS06_06740"
     CDS             <1..659
                     /locus_tag="VHS06_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2987851.1"
                     /translation="DDPKVLSLMKRGLSFAGYTPDVAGTGEEALAIARDRPPALVILD
                     VMLPGMDGVEVCRRMRAGDRDLPILMLTARDRVPDRVAGLDAGADDYLIKPFAFDELL
                     ARIRALLRRTQQEEDKAPSLKFADLTVDPGSREVSRGRRQIELTSREYELLEFFVRHP
                     KQVLTRDTIFEMVWGSDFLGGSNLIDVHVKRLRDKLEANGEDRLLHTIRGAGYSLREK
                     "
     gene            703..1431
                     /locus_tag="VHS06_06745"
     CDS             703..1431
                     /locus_tag="VHS06_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX2987852.1"
                     /translation="MALGYALMMVAIVGVIGLYLESTFENSLLAEIDDALGLRAFHVE
                     RAIAPDGGDSIDRRVTNEALQELASVEEFSEPGIYVQVLAPDGSSITSSPNLPAGGLP
                     GVAGIVLTARPGQEAHITTPVGTDRIRALARPVKSDGQLIGIVVVGESLHLIDVAIRR
                     LQQLLTIVAAVAAVTALFGGWWLTMRALGPITEVTRVARRIASTGHFEQRITPHPTRD
                     ELGELTETFNDMLERLEKTLGLVK"
     gene            complement(1587..2561)
                     /locus_tag="VHS06_06750"
     CDS             complement(1587..2561)
                     /locus_tag="VHS06_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2987853.1"
                     /translation="MPADRVVADARTRIESCRADLDSLLGEAKLGYRKTLMELERLTA
                     QPVDVSGLPLPARLEALRHSQDLNRAAAEYDGGLCRLHAAIACLDDAVEAIEGRIVQR
                     DRDEAAPEMSMEAVAIQAQEEERYRLAREIHDGPAQILANVALQLEYISKLATREPVR
                     ARDELTNIQRDLRMAVGEVRRFMYDLRPPALAQQGVGPAVESHCQRLAERFGMKVQVQ
                     WESTLPFAPSHDTAVFRMVQEALQNVVKHAQATEVQVRAVDKDSTIEVSVSDNGKGFD
                     ATQVRKLDPKHFGLAGMRARAQQIGATLDVTSTPGSGTTIYIRVAAAV"
BASE COUNT          515 a          822 c          828 g          490 t
ORIGIN      
        1 acgatgaccc gaaagtgctg agtctcatga agagggggct ctccttcgcc ggctacacac
       61 ccgacgtagc gggaaccggc gaggaggcac tggctattgc cagggatcgt ccaccagcac
      121 tggttatcct ggacgtcatg ctgccgggca tggatggagt cgaggtgtgc cggcggatga
      181 gggcaggtga ccgcgacctg ccgatcctca tgttgacggc tagggacagg gttccggata
      241 gggtggccgg cctggacgcc ggtgccgacg actacctcat aaagccgttc gccttcgacg
      301 aactgctcgc caggatccga gcactgttgc gacgcactca acaagaggag gacaaggcgc
      361 cctcgctgaa gtttgccgat ctcaccgtcg atcctggaag cagagaggtc tcccgcgggc
      421 gacgacagat cgagctgacc agccgagaat acgaactgct ggagttcttc gtgcggcacc
      481 ccaagcaggt gctcaccaga gatacgatct tcgagatggt gtggggctcc gacttcctcg
      541 gtgggtccaa tctcatagac gtgcacgtca agcggttgcg tgacaaactg gaggccaacg
      601 gcgaagatag actcctccac actattcgag gcgccggcta cagcctgcgc gagaagtgat
      661 ctgggaggaa ctgtgcttca ccgtgtccct ctgaggtggc ggttggcgct cggctatgca
      721 ctcatgatgg tcgccatcgt cggcgtcatc ggtctgtatc tcgagtcgac cttcgaaaac
      781 agccttctgg cggagatcga cgacgccctt gggctgagag cattccacgt agaacgggcc
      841 atcgctcccg acggtggcga ctccatcgac cgtcgggtga cgaatgaggc gctccaggag
      901 cttgcctccg tggaggaatt ctcagaaccg gggatctacg tacaggtgct ggcgcccgac
      961 gggtcctcca tcacctcctc ccccaacctt ccggcaggtg ggctgccggg agtagccggc
     1021 atcgtgctca cagccagacc ggggcaggaa gcgcacatca cgactccagt cggcacggat
     1081 aggattaggg ccctcgcgcg tccggtgaaa agcgacgggc aactgatcgg aattgtcgtc
     1141 gtgggggagt ctctgcacct gatcgacgtc gcgatccggc gactgcagca actgttgact
     1201 atcgtagccg cagtcgcagc tgtaaccgcc cttttcgggg gatggtggct cacgatgcga
     1261 gcactagggc caatcacgga ggtcacccga gttgcccggc gcatcgcatc cacagggcat
     1321 ttcgagcagc gcatcacccc ccatcccacg agagatgagc tcggcgagtt gaccgagacc
     1381 ttcaacgata tgctggaacg gttggagaag acgctggggc tggtaaagta acctcgaggg
     1441 agcaggaacc tggcatttgg gaatccccga agctacttgt tgaacacgga ggctgcgcta
     1501 tctcggcgca gcctccgctc tgtttcatgg ggcaactgaa cagctctccg tggagatcgc
     1561 agctgttcaa cggctatgct cgaggactag accgcagcgg cgactcgaat gtagatggtg
     1621 gtgccggagc ccggcgtcga ggtgacatcc agcgtcgccc caatctgttg ggcgcgagcg
     1681 cgcatcccgg ccaggccaaa atgcttggga tccaacttgc gcacctgtgt ggcgtcgaac
     1741 cccttgccgt tgtccgaaac tgagacttcg atcgtcgagt ccttgtccac tgcccggacc
     1801 tgcacctcgg tcgcctgggc gtgcttcacg acgttctgca acgcctcctg aaccattcgg
     1861 aagactgcgg tgtcgtgaga gggggcaaag ggaagcgtag actcccactg tacctggacc
     1921 ttcatcccaa agcgctcggc cagccgctgg cagtggctct ccacggccgg gccaaccccc
     1981 tgctgcgcga gagccggcgg gcgcaggtcg tacataaagc ggcgcacctc gcccacagcc
     2041 atccgtagat ccctctggat gttggtcagc tcgtccctgg cgcgcacagg ctcccgggtg
     2101 gccagcttgg agatatactc caactgcaga gccacgttgg ccaggatctg tgcagggcca
     2161 tcgtggatct cccgagccag ccgatagcgc tcctcctcct gtgcctggat cgcgacggcc
     2221 tccatagaca tctcgggagc agcttcatcc cggtctcgct ggactatgcg cccctctatc
     2281 gcctccacgg cgtcatcgag gcacgctatg gccgcgtgca atcggcacag cccaccatcg
     2341 tattcggcag ccgcccggtt cagatcttgg ctgtggcgaa gcgcctcgag tcgagcaggg
     2401 agcggcagcc cagaaacgtc cacaggctgg gcggtaagcc tctccagctc catcagagtc
     2461 ttcctgtatc ccaacttggc ctcaccaagg agagagtcca ggtcggcccg gcaagactcg
     2521 atccgagtgc gggcatccgc gaccacgcgg tcggcaggca tgggagattg ctgatcgttc
     2581 ataacttcac tttaactgcc ggaagggagg ccgtcaagca tctgccctgt ggtaaggtat
     2641 agagagtaca cttcg
//
LOCUS       DATALS010000304         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_388775, whole genome shotgun sequence.
ACCESSION   DATALS010000304 DATALS010000000
VERSION     DATALS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388775"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..998)
                     /gene="narI"
                     /locus_tag="VHS06_06755"
     CDS             complement(315..998)
                     /gene="narI"
                     /locus_tag="VHS06_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX2987854.1"
                     /translation="MFDLLLYGVWPYVAFALAIVGGLYRYYRDRFSYSSFSSQLLEQR
                     LLFWGSVPWHYGIVLLLIGHLIGWAIPGGVAAFNGQPLRLYILEITAFGLGLFALLGI
                     VTLVVRRLSVPAARAVTTRMDAILLALLLLQVVLGLFTALFLRWGSWWYLSNAVPYLS
                     SLLRLNPDVRYAATLPLIAQLHTINAFLLVAIFPFSRLVHIFTVPITYLWRPYQTVIW
                     NLRRGVLPW"
     gene            complement(1271..1804)
                     /gene="narJ"
                     /locus_tag="VHS06_06760"
     CDS             complement(1271..1804)
                     /gene="narJ"
                     /locus_tag="VHS06_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEX2987855.1"
                     /translation="MDTRRLLGGLGEMMRYPTPELPEKASEWVAAADCIPADARDAIA
                     RFAAFVESTPPGRLEEVYSASFDLAPACCPYVGHQLLGEDHRRTALLLMLKQCYREVG
                     LELDGELPDHLSLLLCYLAMAGGGEVESELMGRLVLPALEKMVSALEESNGYRALLQG
                     ATALCRAALAESTNAVS"
     gene            complement(1804..>3022)
                     /locus_tag="VHS06_06765"
     CDS             complement(1804..>3022)
                     /locus_tag="VHS06_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2987856.1"
                     /translation="YLPALDDYYEPWSYRYRDLIDAPPGDDQPTARPISMVTDRYLDI
                     EAGPNWDDDLGGSPVYAANDPALVDLTAEERRELFQLERLNFFYIPRICNHCLNPGCV
                     AACPSGALYKRGEDGIVLVSQEKCRGWRFCVSGCPYKKVYYNWTTGKSEKCILCYPRQ
                     EVGLAPACFHACVGRIRYLGPVLYNAEDIEYASSSPDNRLVDAHRNLILDPFDPEVIA
                     EARANGIHDRWILAAQRSPVYKLIKLWRIALPLRDEFRTMPMLFYVPPLLPVMASVGE
                     RGYNLEVEELFAGLEQSRIPFRYLARLFGAGNREVVREALAKLVAVRVYMRSKTVGDV
                     RATLVQETLREAGLAEEEAEAIFRLTTVPGPGERHAVPPYQREMAIQESEAPNIHRGE
                     TGLGTRQPPWRRF"
BASE COUNT          573 a          963 c          970 g          516 t
ORIGIN      
        1 taacccttcc tctcaggcac tacccgttcc gccgcgatga tgagaagggg actgtgccct
       61 tcccacaaca gaaaggatag atgggagggc ggggaattgc tgtgactttt gtcaccaagg
      121 gacctggatt tggagtgtgg cgcggtaggg gcgaggcatg cctcgccctg gctttcgtgg
      181 gggaggcgtg agtcgacccc gaagcgtgaa ccgggccgaa tctgggagcg ccgtctcctc
      241 agtcgacagg gtgtgcaggg caacattgtg acgtcaagcc gctgcactcc agaagctgcc
      301 tgcgggtcag atccttacca cggcagaacc cctcgccgca ggttccagat caccgtctgg
      361 tatgggcgcc acaggtaggt aatcgggacg gtgaagatgt ggaccagccg ggagaagggg
      421 aagatcgcca caagcaggaa ggcgttgatg gtgtgtagtt gggcgatcag cggtagggtt
      481 gccgcgtatc gcacgtcggg attcagcctc agcagtgagc tgaggtaggg tactgcgttc
      541 gagaggtacc accaggagcc ccatcgcaag aagagggcgg tgaacagtcc gagcaccacc
      601 tgaagcagca gcaacgccag cagtatggcg tccattcggg tggtgacggc cctcgccgcc
      661 gggacgctga gccttcggac caccagtgtg acgatgccga gaagggcgaa cagccccaga
      721 ccgaaagcgg tgatctcgag gatgtagagc cgcaggggct gcccgttgaa ggcggcgacc
      781 ccgcccggga tggcccaccc gatcaggtgg ccgatcagca gaaggacgat gccgtagtgc
      841 cagggaaccg atccccagaa cagcagccgc tgctccaaca gttgtgagga gaagctcgag
      901 tagctgaagc ggtctcgata gtagcgatac agcccaccca cgatggccag cgcgaacgcc
      961 acgtagggcc acaccccgta cagcagcaga tcaaacatgt tgagcctcca tgcatcacgg
     1021 cttgatgcgc cgtagcttcg cgcctgatgt cgtgtctatg gctgcgggca ccgaaatgta
     1081 gcgtcggggc ttgtctcgac gtcgtactcc gtcgaacagg ctacccgtcg gggacaagcc
     1141 ccgacgctac agaagcgtgg tcctaatcca ccatagtggt cgtagccgct gcgaaggcgg
     1201 cggggcacaa gaccccgccc tacgagacgc aataggtggc ctcgaccacc atgacaggcc
     1261 gatacgtgcc ctatgagacg gcattggtcg actccgccag cgcggcacgg cacagcgccg
     1321 tggcaccctg gagaagcgcc ctgtagccgt tgctctcctc cagcgcggat accattttct
     1381 ccagcgctgg cagcaccagg cggcccatca actccgactc gacctccccg cctcctgcca
     1441 tcgccaggta gcacagcagc agcgagaggt ggtccgggag ttccccgtcc aattctagcc
     1501 cgacctcccg gtagcactgc ttgagcatca gcaggagcgc agttcgcctg tgatcctcgc
     1561 ccaacagttg gtgcccgacg taggggcagc atgccggcgc gaggtcgaaa ctggcgctgt
     1621 acacctcctc caagcggccc ggcggcgtgg actcaacgaa ggcggcaaac cgggcaattg
     1681 cgtctctagc gtccgccggt atgcagtcag ccgcggctac ccattcgctg gccttttcgg
     1741 gcaactcagg agtggggtac cgcatcatct cccccagccc tcccagcagc cggcgtgtat
     1801 ccatcagaac ctcctccagg gcggctgccg tgtgcccagg ccggtctctc ccctatggat
     1861 gttgggggct tcgctctcct ggatggccat ctctcgttgg taggggggga cggcgtgacg
     1921 ctcgccggga ccgggtacgg tggttagccg gaagatcgct tctgcctcct cctcggcgag
     1981 ccctgcctcc ctcagcgtct cctgaaccag ggttgccctc acatctccca ccgtcttgga
     2041 gcgcatgtag acgcgcacgg cgaccagttt ggccagcgcc tctcgcacca cctcccggtt
     2101 gccggccccg aagagccgtg ccaggtatcg gaagggaatc ctcgattgct ccagccccgc
     2161 gaacagctcc tccacctcca ggttgtaacc ccgttccccc acagaggcca tgacgggcag
     2221 caggggcggg acgtagaaca gcatcggcat ggtacggaac tcgtcacgaa ggggcagggc
     2281 tatccgccac agcttgatca gtttgtagac aggcgaacgc tgcgcggcca ggatccacct
     2341 gtcgtggatc ccgttcgccc tggcctcggc gatcacctcc ggatcgaagg ggtccaggat
     2401 caggttgcgg tgggcatcca caagccggtt gtcggggctc gacgaggcat actcaatatc
     2461 ctctgcgttg tagagcaccg ggcccaggta ccgaatacgg cccacgcagg cgtggaagca
     2521 ggccggagct aagccaactt cctgacgggg gtagcagaga atgcacttct ccgactttcc
     2581 ggtggtccag ttgtagtaca ccttcttgta ggggcagccg gacacgcaga agcgccaccc
     2641 gcggcacttt tcctgactca ccagcactat gccatcctcg ccccgcttgt acagggcacc
     2701 ggatggacag gcggcaacgc accccgggtt cagacagtgg ttgcagatgc gcggtatgta
     2761 gaagaagttc agcctctcca gctggaacag ctcccggcgc tcctcggcgg tcaggtcgac
     2821 cagcgcgggg tcgttggcgg cgtagaccgg cgacccgccc aggtcgtcgt cccagtttgg
     2881 gccggcctcg atatccaggt accgatccgt gaccatggag atggggcgtg cggtgggctg
     2941 atcgtcgccc ggcggcgcat cgatcaggtc cctgtagcgg tagctccagg gctcgtagta
     3001 gtcgtccagc gcgggcaggt ac
//
LOCUS       DATALS010000305         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_215591, whole genome shotgun sequence.
ACCESSION   DATALS010000305 DATALS010000000
VERSION     DATALS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215591"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..624
                     /locus_tag="VHS06_06770"
     CDS             265..624
                     /locus_tag="VHS06_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0567/Sll0786 family nitrogen starvation
                     N-acetyltransferase"
                     /protein_id="HEX2987857.1"
                     /translation="MTPCTSWQWTRTQVRSWEPCVATRSGGGTWYGGRLAVLKQYRRV
                     SGLTGCHLVKMAEDVVRQRGCTRFLAYVQLQNVRFFQHMGWRSLGEPEEHYGQPHMVM
                     EANLMPSTVAVRSLVHA"
     gene            617..1630
                     /locus_tag="VHS06_06775"
     CDS             617..1630
                     /locus_tag="VHS06_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sll0787 family AIR synthase-like protein"
                     /protein_id="HEX2987858.1"
                     /translation="MPDLALNSIVATLRAAPAWQRKNEISLLRDSLVPHAPMVDGRPV
                     LVGDDTAAIETEDGYLLLAAEIMYTPLVKSNPYLAGNYAVLANTNDVYAMGGYPIALV
                     DVILAQDTEGAADLLQGLRDGCSRYGFPLVGGHLTANGDFSSVGACILGKAKRILSSF
                     NARPGDALLHATNLNGAFHPRYTFFNCTDHLPDAELRCDLAILPTLAEAGLCDACRDV
                     SMAGILGSAMMLLEPSGVGAVIDLQAIPRPPQAVARFIDWLLAFPSYGFILSVRPEHV
                     VAVRGAFASRSIACAVIGEVTASREVIVRDGPEESLLWDFGMEPFMGFSPNQHKNGTT
                     IRR"
     gene            1633..>2820
                     /locus_tag="VHS06_06780"
     CDS             1633..>2820
                     /locus_tag="VHS06_06780"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2987859.1"
                     /translation="MTRIVVIGGGPGGTAAATNAIQKDRSASVTLITDFEDIAYSPCG
                     IPYAFGREVPTFESLFLQNADFYAKMGLDLRTSTTVTRIDAQEKVVFTDNGQFPYDKL
                     VIATGWGYEVPAVPGAGLKGIWYLKNIRRAMEIDPTLDQCKSALVYKGKPLATEMAFA
                     LTHRGIQVHVVDEYPWLCADFVDPEIMKPMQDALEKMGVQFHLGQHLEEIKGTNGKVS
                     SFRTGDGEFPCDVVFVTGPVKPNTDLARSIGVKCGSTGGVVVDDHMRTNVPDVYAVGG
                     CVEIMHGCLNIPIHILPSTFAYPQGRIAGINAVGGNLAYYPVFGPWGMIVGNVQASGV
                     TICETMAKALGLPYFIGQATGLTAARYHPASEKLFVKLIADTKTHRLIGAEMVGGEGA
                     KERA"
BASE COUNT          537 a          827 c          900 g          556 t
ORIGIN      
        1 ctgatggcat ctatctacac tcaggtagcg gctatcaacc gagaacaggg attgagttgg
       61 cgcaacagca aagcgggatg tgagcgctgt ggggcttgct ctgcgttgcc ggcctacgag
      121 ggctgaggga ggtagagatg gccagcatcg tctacaggat agccgagacc gaaaaggagc
      181 ttcagggcca ctatgctgtg cggcacgcca tctttgccgg ggagcaggga ctcttcgtgg
      241 gctctgatcg agacgagcac gacgatgacg ccgtgcacat cgtggcagtg gaccaggaca
      301 caggtgaggt cgtgggagcc gtgcgttgct acgaggtcgg gggggggcac ctggtacggc
      361 ggacggctgg ccgtattgaa gcagtatcgc cgggtgtcgg gactgaccgg gtgccatctg
      421 gtgaagatgg ccgaggatgt ggtccggcag cggggctgta cccgttttct cgcctacgtg
      481 cagcttcaga acgtgcgttt cttccagcac atgggttggc gctcgcttgg agagccggaa
      541 gagcactacg gacagccgca catggtcatg gaagcgaatc tcatgccctc tactgtagcg
      601 gtgaggtctc tagttcatgc ctgatctcgc tttgaacagc atcgtcgcaa ccttgcgggc
      661 cgcacctgcc tggcagcgga agaacgagat ctccttgctg agggattctc tcgttcctca
      721 tgcccccatg gtcgatggaa ggccggttct tgtgggtgac gacaccgccg caatcgagac
      781 cgaggacggg tatctgttgc tggcggctga gatcatgtac acaccgctgg tgaagtccaa
      841 tccctatctg gcgggcaact atgccgtcct ggccaacacc aacgatgtct acgccatggg
      901 cggctatccc attgccctgg tcgatgtgat cctggctcag gataccgagg gcgccgccga
      961 tctgttgcag ggtttgcgcg acggctgttc tcgatacggt ttccccctcg tgggtggaca
     1021 tctcacggcc aacggagatt tcagctctgt cggggcctgc atactgggta aggcgaagcg
     1081 aatcctgagc agcttcaacg ccagacccgg cgacgcgctg ctccatgcca ccaacctgaa
     1141 tggggccttt cacccgcggt acaccttctt caactgtacc gaccacctgc cggatgccga
     1201 gttgcgatgc gatcttgcca tcctgcccac cctggccgag gctggtttgt gcgatgcctg
     1261 cagggacgtc agcatggcgg ggatcctggg atcggcgatg atgctcctgg agccctccgg
     1321 cgtgggtgcg gtgatcgacc tccaggccat accgcgaccg cctcaggcgg tcgctcgttt
     1381 catcgactgg ctgcttgcct tccccagcta tgggttcatc ctgtcggtgc gtcccgagca
     1441 cgttgtggca gttcggggcg cctttgcttc ccgttccatt gcctgtgccg tgataggcga
     1501 ggttacagcc tctcgtgagg tcatcgttcg ggatggcccc gaggagtcgc tattgtggga
     1561 tttcggaatg gagccattca tgggcttctc tccaaaccag cataagaacg gaacaacgat
     1621 caggaggtaa ttatgacccg gatcgtcgtc atcggtggag gtcccggggg gacggctgca
     1681 gcgacaaacg ccattcagaa agatcgctca gccagcgtta ccctgattac cgacttcgag
     1741 gacatcgcct atagcccctg cgggataccc tacgctttcg gacgcgaggt tccaactttc
     1801 gagagcctct tcctgcagaa cgccgacttc tacgccaaga tggggctgga cctgcgcacc
     1861 agcacaacgg tcacacggat cgatgcgcag gagaaggtag tcttcaccga caatggccag
     1921 tttccctacg acaagctggt gattgccacc ggttggggat acgaagttcc tgccgttcct
     1981 ggtgccggcc tgaaggggat ctggtacctc aagaacatcc gacgtgctat ggagatagac
     2041 ccgacgctgg accagtgcaa gagcgcgctc gtctacaagg gcaagccgct cgcaaccgag
     2101 atggcatttg cactgaccca ccgtgggatt caggtgcacg tggtagacga gtacccctgg
     2161 ctctgcgccg acttcgtcga ccctgagatc atgaagccga tgcaggacgc gctggagaag
     2221 atgggcgttc agttccacct gggccagcac ctggaggaga tcaagggtac caacggcaag
     2281 gtttcatcat tccggaccgg cgatggggag ttcccctgcg acgtggtatt cgtgaccggg
     2341 ccggtgaagc ccaacaccga tctcgcccgc tccatcggag tcaagtgcgg atccaccggt
     2401 ggggtcgtcg tagacgacca catgcggacc aacgtgccgg acgtgtacgc cgtgggtggc
     2461 tgtgtcgaga taatgcacgg ctgcctgaac atcccaatcc acatactccc cagcaccttc
     2521 gcctacccgc aggggaggat cgcagggatc aatgcggtgg gcggcaacct ggcctactac
     2581 cccgtcttcg gcccctgggg gatgatcgtc ggaaacgtcc aggccagcgg cgtcaccatt
     2641 tgcgagacca tggccaaggc gctcggtttg ccctacttca tcgggcaggc gacggggctg
     2701 accgcggcgc gctaccaccc tgccagcgag aagctgttcg tgaagttgat cgccgacaca
     2761 aagacgcacc ggttgatcgg cgcagaaatg gtcggcggcg agggggctaa ggagcgggcc
//
LOCUS       DATALS010000306         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_284967, whole genome shotgun sequence.
ACCESSION   DATALS010000306 DATALS010000000
VERSION     DATALS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284967"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VHS06_06785"
     CDS             <1..243
                     /locus_tag="VHS06_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987860.1"
                     /translation="WSCGDEFLLRHIPHAYPEAALDVLRSLQHQLNECDDILKAVIQD
                     VDALVTDALATYGRGRFITRCDREQCCRGFCIYVQE"
     gene            complement(612..>1509)
                     /locus_tag="VHS06_06790"
     CDS             complement(612..>1509)
                     /locus_tag="VHS06_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX2987861.1"
                     /translation="VAYALQDGAVHSVNVDGSGDRVLARVKSGPGELDALEFSRDGSI
                     VRVSRRYGEDKCYFINTRDGTVTERRAKDRLDALRSEWQIGTDAFLGQDLQFVRVVRT
                     DRSKGAAVAPATATRPVGLPQGGKVERNQTTDLDGDGTLETAYFAAAQGGHALWVEKD
                     QAVVWKASDRFSSDYYWVYCMDTIDVDGDGRPEICIFQSPGDVGSGAFHAYTWHKGGF
                     TPVTNLAGVRPMYKDATRGVPAQFAILRTSSTGASTLHRYRWDGQGFAPTSAGQVEGS
                     APRAAAEAMHREGFRDIPVDQE"
BASE COUNT          280 a          484 c          419 g          326 t
ORIGIN      
        1 tggagctgtg gcgacgagtt tttgctgcgg catatcccgc acgcataccc ggaggcggcg
       61 ctggacgtat tgcgcagctt acagcaccag ttgaatgagt gtgacgacat cttaaaggct
      121 gttatccagg atgtagatgc gttggtcaca gacgcacttg ccacctatgg gcggggacgt
      181 ttcattacca ggtgcgaccg cgagcaatgt tgccgaggct tctgtatcta cgtgcaagaa
      241 tagggcaggg aataaacgcg atagcggtgg gcggtatggc acctcgccgc ctacctggtc
      301 ctccacaggc ggatcggcaa ccgggtgaac acgacgcgca tcatcaacca cgccctcgcc
      361 ttggcagcac ctgtacgtct gcttgagacc aggctggatg aatggcctac gcagtcagtc
      421 actgacctgc gaggtgagac catccctcga ctgcctctca ctcgttgcat caggcgacga
      481 gttccccgtc ccggtcatct cgcgcgttcc accaaggacg ccaagtgaac ttgctacgaa
      541 ttagtggaca cctctctatc tcctgttcgc ccttgtagga catgtgtcca ctaatacgga
      601 gcaagttcac ttcattcctg gtcgaccggg atgtcacgga acccctctcg gtgcatggcc
      661 tcggcggcag cgcgtggcgc gctaccctcg acctgtcctg cgctcgtggg cgcgaacccc
      721 tgcccatccc acctgtagcg atgcagagtg cttgcgcccg tgctagatgt cctgaggatc
      781 gcgaactggg cagggactcc acgcgttgca tccttgtaca ttggcctgac tcccgccagg
      841 ttggtaaccg gcgtgaagcc acccttgtgc catgtatagg cgtggaaagc cccgctgcca
      901 acatcgccgg ggctttggaa gatgcagatc tccgggcgac cgtcaccatc gacgtcgata
      961 gtatccatac agtagaccca gtagtagtcg ctgctgaacc tgtccgatgc cttccagacg
     1021 actgcctgat ccttctcgac ccacagcgcg tgaccgcctt gtgctgcggc gaagtaggct
     1081 gtctccagtg ttccgtcgcc atcaaggtcc gttgtctggt ttctctcgac cttgccgccc
     1141 tgcggaagcc ccacgggtcg ggttgcagtg gccggggcta ccgctgcgcc cttgcttctg
     1201 tcagtacgga caacgcggac gaactgcaag tcttgaccga gaaacgcatc ggtaccgatc
     1261 tgccactccg atctcagtgc gtcgagtctg tccttcgctc gccgctccgt tacggtgccg
     1321 tcgcgagtgt tgataaagta gcacttatcc tctccatacc ttcggctcac ccgaacgatg
     1381 ctaccatctc tgctgaactc cagagcgtca agctcccctg ggccgctctt gacacgagcc
     1441 aggactcggt cgccgctccc atctacgttc accgaatgga ctgccccatc ttgaagcgca
     1501 tacgctacg
//
LOCUS       DATALS010000307         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_215982, whole genome shotgun sequence.
ACCESSION   DATALS010000307 DATALS010000000
VERSION     DATALS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215982"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VHS06_06795"
     CDS             <1..217
                     /locus_tag="VHS06_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987862.1"
                     /translation="LGDRDLVDAQVVAVHLDQLQGMYGYDCRTFCSAMQPSLFVDLEV
                     LLEGFSRHKEEMPVLPSLTQISSATSR"
     gene            complement(320..397)
                     /locus_tag="VHS06_06800"
     tRNA            complement(320..397)
                     /locus_tag="VHS06_06800"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(360..362),aa:Pro,seq:cgg)
     gene            complement(562..2457)
                     /locus_tag="VHS06_06805"
     CDS             complement(562..2457)
                     /locus_tag="VHS06_06805"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987863.1"
                     /translation="MKVRLYSFLLPLMLVASTLTGFGSQGMAVAAPPAPAQEIIPQTQ
                     LPWRDLADLAVRLKGVSPDSVNQQPPQDVPEKQVGDKASFWVADQSNNRYYSVDATLK
                     VVTPHAYMYVADDSMVDIPSLQEAAQGFEDRVYAADRHYFGSEPALGLDGDAHISILH
                     ATIPGLGGYFTSVDDYPRAVQGYSNERKVIYINIQAAPPASLGYYSILAHEFEHMIHW
                     NTNRAEQTWVKEGAAEEATEATSLGGGSSVRAFENASDTQLDAWADSQGDVAPHYGAA
                     YLFLSYFLEHYGGYEGAADLLTGDTRGPDTFDKYLSTHGYTQSFEDVFKDWVVANYLD
                     TRAVDPRYRYGNVKVQLPATDRVNAPTREARKSVHQFAADYIELGGGFSSATLHFKGD
                     QSTRVISANAPGGSSFWWSNRGDLVDTKLTRDFDLTGVSGATLNFSAWYDLEDGYDYG
                     YVMVSEDGGATWKTLSSPDTTSDDPNGNNLGQGFTGKSRGADSGQWVQESMDLTPYAG
                     KEVMVRFEQVTDDAYNAPGFAVDDISVPEIGYSSGAELDEDGWTADGFIRTDGRLPQP
                     FSLQLIQMNGNNLSVDQIPISEDGTADVVVKNPNGDLNKAVVVVSALSRYTTEVAGYR
                     YSVDMTR"
     gene            complement(2576..>3538)
                     /locus_tag="VHS06_06810"
     CDS             complement(2576..>3538)
                     /locus_tag="VHS06_06810"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX2987864.1"
                     /translation="RRAQVAATTMVTTITLMAGHKTPPYDMPMVAECHRALDKGNPLE
                     YPMAELERAVELVSSITAVRPRVGIVLGSGLGPVAEGIEGAVTIPYRQIPGFPSTTVG
                     GHRGELVLGTLDGLEVAALMGRPHLYEGYVPTQLGFPIQLLGALGAEVLVVTNAAGGL
                     TPNLEPGTLMLIEDHVNLPGIGGHNPLVGIGGGNERFVPMAGAYDEELRRVALEAAAE
                     RGIRAATGVYVMVAGPNYETAAEARFLRLLGADAVGMSTVPEVLVARWLGMRVVGISC
                     ITNSLLGPPSHAVDGHEGVLSVAATAAPGMAHLVRSIVRYLARM"
BASE COUNT          663 a         1126 c         1106 g          643 t
ORIGIN      
        1 cctcggagat cgggatctgg tcgacgctca ggttgttgcc gttcatctgg atcagctgca
       61 ggggatgtac gggtatgact gccgcacctt ctgttctgcc atgcaaccct ccttgtttgt
      121 cgatcttgaa gtgctgcttg aaggcttcag ccgccacaaa gaagaaatgc cagtgctacc
      181 atcgctgacg caaataagta gtgcgacatc ccgttagata ccataacggt gttgctggca
      241 agcgtggttg aagaggagac tactggacca gattggcagc agtagggccc tcaagcacgc
      301 caatgcctga aggccctctt ggtcggggcg agtggatttg aaccaccggc ctcagcgtcc
      361 cgaacgctgc gcgctaacca ggctgcgcta cgccccgatt acccgttgat tctaccatac
      421 ctccacggta tgggcaaact gcggaccacc tctgcgctcc agaccgtccc aggccggggg
      481 agcacccacc agccacacgg gtgctcggtc ttccatcaca tcccagccct cagtgtcgcc
      541 ctcgaaggat catccccacg tctaccgggt catgtccacc gagtaccggt agcccgcgac
      601 ctcggtcgta tagcgcgaga gagcggagac gaccaccacc gccttattca ggtcgccgtt
      661 ggggtttttc accaccacgt cggcagtgcc atcctcggag atcgggatct ggtcgacgct
      721 caggttgttg ccgttcatct ggatcagctg cagggagaag ggctggggca gcctgccatc
      781 ggtgcggatg aagccatcgg cagtccagcc gtcttcatcc agttccgcgc ccgagctgta
      841 gccgatctcc ggcacggaga tgtcgtccac ggcgaagccc ggggcgttgt aggcgtcgtc
      901 ggtcacctgc tcgaagcgaa ccatcacctc tttccccgcg tagggagtca ggtccatcga
      961 ctcctgcacc cactggccgg agtcggcgcc gcggctcttg ccggtgaacc cctggccgag
     1021 gttattgccg ttgggatcgt ccgaggtggt atcgggcgac gagagggtct tccaggtggc
     1081 cccgccgtcc tcggacacca tcacgtagcc gtagtcgtag ccgtcctcca ggtcgtacca
     1141 ggccgagaag ttcagggttg caccggacac accggtcaga tcgaaatcgc gggtcagctt
     1201 cgtatccacc agatcgcctc gattgctcca ccagaagctg ctgccgccgg gggcattcgc
     1261 ggagatgact cgagtgctct gatcgccctt gaagtggagg gtggcgctgg agaagccacc
     1321 tcccagctca atatagtccg ccgcgaactg gtgaacgctc ttgcgagctt cgcgagtcgg
     1381 ggcgttgacc cggtcggtgg ccggcaactg aaccttcaca ttgccgtatc ggtagcgcgg
     1441 atcgacggcg cgggtgtcca ggtagttggc cacaacccag tccttgaaaa cgtcctcgaa
     1501 gctctgggtg tagccgtgcg tcgaaagata cttgtcgaag gtgtcggggc cacgggtgtc
     1561 gccggtgagg agatcggccg caccctcgta gccgccgtag tgctccagga agtagctcaa
     1621 gaagaggtag gccgcgccgt aatggggtgc cacgtcaccc tgactatcgg cccatgcgtc
     1681 caactgggta tccgaggcgt tctcgaaagc acgaacagag ctgcctccgc ctaaactggt
     1741 tgcctccgtg gcctcctcgg cggcgccctc cttgacccag gtctgctccg cccgattggt
     1801 attccagtgg atcatgtgct cgaactcgtg agcgaggatg gagtagtagc ccagggaggc
     1861 agggggcgca gcttgaatgt ttatgtaaat aaccttgcgc tcgttgctgt agccctggac
     1921 ggcacgaggg tagtcgtcga cggaggtgaa gtagcccccc aggccgggga tggtggcgtg
     1981 cagaatggag atgtgggcat cgccgtccaa ccccaacgcg ggctcgctgc caaagtagtg
     2041 ccgatcagca gcgtagaccc gatcctcgaa gccctgggcc gcctcctgca aactggggat
     2101 atccaccatg gagtcgtcgg ccacgtacat ataggcgtgt ggagtcacca ccttcagggt
     2161 ggcgtccaca gagtagtagc ggttgttgga ctgatccgcc acccagaagg aggccttatc
     2221 cccaacctgc ttctcaggga cgtcctgcgg gggctgctga tttaccgagt cgggcgatac
     2281 cccttttagc cgaacggcca gatccgccag atcccgccag ggcagttggg tctggggaat
     2341 gatctcctga gcgggcgccg gaggggcagc cacagccatt ccctgggaac caaacccggt
     2401 gagggtgctc gctacgagca tgagcggtag caggaaggag taaagtctca ctttcatttc
     2461 actgcccctc ataagaatcg gttttgagac tgctgaaccc gggaagacgg ctgcgggtaa
     2521 gcaagatagg gcgaacggct actgggcaac gaactccaga acccagcact caacactaca
     2581 ttcgggccag gtagcgcacg atcgaccgaa caagatgggc catcccgggc gcggcggtcg
     2641 ccgcgacgga gaggactccc tcgtgcccat cgacggcgtg gctcggcggg ccgagcaacg
     2701 aattggtgat gcaggagatg cccaccactc gcatccccag ccatcgcgcg accaacacct
     2761 cgggcaccgt ggacatcccg accgcatctg ccccgagaag gcgcaagaag cgagcctcag
     2821 cggcggtctc gtagttgggg ccggcgacca tcacgtacac gccggtcgcc gccctgatcc
     2881 cccgttcggc ggcagcctcc agggcgacgc gacgcaactc ctcgtcgtag gcgccggcca
     2941 tcggcacaaa gcgttcgttt ccgcccccta tgcccaccag agggttgtgg ccgccgatgc
     3001 ccggcaggtt gacgtgatcc tcgatcagca tcagcgttcc gggctccagg ttgggggtca
     3061 gccccccggc tgcgttggta accaccagca cctcggcgcc cagcgccccc agcaactgaa
     3121 tgggaaaccc aagttgggtg gggacatacc cctcgtacag gtggggccgg cccatcaggg
     3181 cggcgacctc caacccgtcg agggtgccca gcaccaactc gcctcggtgg ccgcccaccg
     3241 tcgtggaggg gaagcccgga atctgccggt aggggatagt gacggcgccc tcaatcccct
     3301 cggccaccgg tccgagaccg gagccgagca cgatcccgac ccgcggccgg accgcggtga
     3361 ttgaggagac cagttcgacg gccctttcca gctcagccat ggggtactcc aaaggattgc
     3421 ctttgtcgag ggctcgatga cattcagcca ccatcggcat gtcgtagggc ggggtcttgt
     3481 gccccgccat cagcgtgatc gttgtcacca tggtggtcgc agccacttga gcccgtct
//
LOCUS       DATALS010000308         4708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_216031, whole genome shotgun sequence.
ACCESSION   DATALS010000308 DATALS010000000
VERSION     DATALS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216031"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VHS06_06815"
     CDS             <1..314
                     /locus_tag="VHS06_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987865.1"
                     /translation="FLLPVGVGTITMGIIGFTTGLGEANMYRTNFIISLVAIFLATVF
                     YHSVVMLSLQAVGWNVEWISTLALQTIPGAVFNAALAPLVFPFVRRLGAAGGEEEQMR
                     W"
     gene            308..2233
                     /gene="mrdA"
                     /locus_tag="VHS06_06820"
     CDS             308..2233
                     /gene="mrdA"
                     /locus_tag="VHS06_06820"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX2987866.1"
                     /translation="MVESGPVKWSAITAHMEKEKKQGPDWRLWALRFTIAVAFAVLLA
                     QLWRLQIVEGHSFRQLADINRFRVSPVAAPRGVIYDRNHKIVAANTPSFQVAIVPAAL
                     PKDNPGRVYSELGNMLNIDPAEIADKVTRRHGDDFTPVTIKSDVERDVVLRVEERHLR
                     LPGVIVQPESSRLYPYGTLLSHILGYMLPITEEQLAANQSDKQSGYVAEDRVGATGLE
                     SSYEKDLRGTPGKKLYEVDATERPVADLRIDNPDPGHNLALSIDVDLQKDVARILSDG
                     MGKSLSAAAIVMDPRNGQVIAMVSIPSYDNNLFSSKVKDSDLQALLNDPGKPMIDYAM
                     GGTFAPGSTYKLVTAAASLQEGVANANTRILCQGALIIPNQYNPALSQRLPCWGVHGL
                     QDFVTGLANSCDVYYWTLGGGFKDFKGVGNERLAKWGNLMGYGTPTGIDIPGEAKGLV
                     PTADWKKETWSEDWLTGDTYNMSIGQGFVLATPLQVANATNAIANGGHLYKPKLVASI
                     RDSEENLLKNVEPEEVRQIPLSQENLDIIKAGMKIVPTNDDVKNVNIPALKIAGKTGT
                     AEFPGERDAKGILPTHGWFTAFAPYDDPQVSVTVFVQRGGGPSDAAPIAMKIFARYFN
                     YSAPTAAPTPTPQQTGR"
     gene            2247..3374
                     /gene="rodA"
                     /locus_tag="VHS06_06825"
     CDS             2247..3374
                     /gene="rodA"
                     /locus_tag="VHS06_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX2987867.1"
                     /translation="MIASILRRWRYADFLLLFILLLILAYGLALVYSATFPSTNTETI
                     AFSSYFVTQVVYGVVGILVLVLMASTDYRLLYASAYVIYVAVLLLLAVVLFTAHGQAE
                     YGSQRWIDLKVFRLQPSELAKPALVIALARYYADRQPAIGALRHYLGSVLLMAPPVVL
                     VYAQPDLGTSSTFVVIWFLMSLAAGVRVVYLGITAVLGFASIPVIWSLLKEYMRDRIT
                     IFLHPESDPWGQGYNIIQAQISIGSGGMTGRGFLAGTQSQGHFLRIQYSDFIFSVLAE
                     ELGFIGAVVLFALFLLLLLRILRIAGRARDGFGRLLAAGIVVMLLFSITVNIAANLRL
                     MPVTGIPLPFISYGGSSLLTNLACIGIVQSVLMRRKRFLPG"
     gene            3726..4571
                     /locus_tag="VHS06_06830"
     CDS             3726..4571
                     /locus_tag="VHS06_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX2987868.1"
                     /translation="MGFLSSAGTGFLILLVLAIFLFMSAVKVVQEYERGVIFRLGRLI
                     GAKGPGLFFIIPGIDKMMKVDLRTVTMDVPAQEVITRDNVTVKVNAVIYFKVVDPNQA
                     VVKVLDYIRATSQIAQTTLRAVLGQSELDELLAQRDKINRHLQQIIDEQTEPWGIKVS
                     VVEIKDVELPQTMQRAMAKQAEAEREKRAKVIHAQGEFDASARLADAADQLNRTPTAL
                     QLRYMQTLTEIAVEKNSTIIFPLPIDILTGVLPNLTKMNGHAPASEVPPASPMDPLEP
                     RESRE"
BASE COUNT          965 a         1502 c         1341 g          900 t
ORIGIN      
        1 gtttccttct tccagtcggc gtcggcacca tcaccatggg gatcatagga tttaccaccg
       61 gcctcggcga ggccaacatg taccgcacca acttcatcat ctccctggtg gccatcttcc
      121 tggccaccgt cttctaccac agtgtcgtta tgttgagtct tcaggcggtc ggatggaatg
      181 tcgagtggat cagcaccctc gcgttgcaga ccatcccggg cgccgtcttc aatgccgcgc
      241 tcgcccccct ggtcttccca ttcgtccgcc gacttggggc cgcaggcggg gaagaggagc
      301 aaatgcgatg gtagagtcgg gtccggtcaa gtggagcgcg atcaccgccc acatggaaaa
      361 ggagaagaag cagggcccag actggcggtt gtgggcgctg cgtttcacca tcgccgttgc
      421 cttcgctgtt ctgctggccc aactgtggcg gttgcagatt gtggagggtc acagctttcg
      481 ccagttggcc gatatcaatc ggttccgcgt cagcccggtt gctgccccca ggggcgtgat
      541 ctacgatcgt aaccacaaga tcgtagccgc aaacaccccc agcttccagg tagccatcgt
      601 tccggccgca cttcccaagg acaacccggg ccgcgtttac agtgaactgg gtaacatgct
      661 caacatcgac ccagccgaga tcgccgacaa ggtgacccgc cgccacggcg acgatttcac
      721 cccggtcacc atcaaatccg acgtggaacg cgatgtggtg cttcgagtcg aggagcgaca
      781 ccttcggtta cccggcgtca tcgtccaacc cgaatccagc cggctgtatc catacggcac
      841 gttactctcg cacattctgg gctacatgct ccccatcacc gaggagcaac tggcagccaa
      901 ccagtccgac aagcaatccg gatacgtggc tgaagatcga gtcggggcca caggattgga
      961 gtccagctac gagaaagacc tgcgaggaac gccgggcaag aagctgtacg aggtggacgc
     1021 cacagagcgg ccggtagccg acctgcgtat cgataacccg gaccccggac acaacctcgc
     1081 tctgagcatc gacgtggacc ttcagaaaga tgtcgccaga atcctcagcg atggcatggg
     1141 caagtcgctg tcggccgcgg ccatcgtgat ggatccacga aacggccagg tcatcgcgat
     1201 ggtcagcatc cccagctacg ataacaacct cttctccagc aaggtgaagg attccgacct
     1261 ccaggcactg ctgaacgacc ccggcaagcc gatgatcgac tacgccatgg gaggcacctt
     1321 cgcgcccggt tcaacctaca agctggttac tgcggcggcc tccctccagg agggcgtagc
     1381 caacgccaac actcgaatcc tctgccaggg ggcactcata attcccaacc agtacaaccc
     1441 ggccctcagc cagcgactcc catgctgggg cgtccacggc ctccaggact tcgtcaccgg
     1501 cctggccaac tcttgcgacg tctactactg gacgctgggg ggcggcttca aggacttcaa
     1561 gggagtgggc aacgaacggt tggccaagtg gggcaacctg atgggctacg gcacgccgac
     1621 cggtatcgac atccccggag aggccaaagg actggtgcca accgccgact ggaagaagga
     1681 aacctggagc gaggactggc tgaccggcga tacctacaat atgtccatcg gccagggctt
     1741 cgtgcttgcc acgccgctac aggtagccaa tgcgaccaac gccatagcca acggcggcca
     1801 cctatacaag ccaaagctgg tcgcctccat ccgagactca gaagagaacc ttctcaagaa
     1861 cgtcgagccc gaggaagtcc gccagatccc gttaagccag gaaaacctgg acatcatcaa
     1921 ggcgggaatg aagattgtcc cgaccaacga cgacgtcaag aatgtcaaca tcccggccct
     1981 caagatcgcc ggcaagaccg gtaccgctga gttcccgggt gagagggatg ccaagggcat
     2041 cctgccaacc cacggttggt tcacggcctt cgcgccgtac gatgatcccc aggtctcagt
     2101 aacggtgttc gtccagcggg gtggtggtcc gagcgacgct gcgcccatcg ccatgaagat
     2161 cttcgccagg tacttcaatt actcggctcc aactgcggcc cccacgccga cgccacagca
     2221 gaccggcagg tagaggagta tcggacatga tcgcatccat actcaggcgc tggcgctacg
     2281 ccgatttcct gctgctcttc atactgctgc tgatcctggc ctacggcctg gcgctggtgt
     2341 acagcgccac cttcccgtcc accaataccg aaaccatcgc cttcagcagc tactttgtaa
     2401 cccaggtcgt atacggggtc gtggggatcc tcgtcctggt gttgatggcg tcgaccgact
     2461 atcggctcct gtacgcctct gcctacgtga tctacgtggc cgttcttctg ctgctcgcag
     2521 tcgtgctctt cacggcccac ggccaggcag agtacggatc gcagcggtgg atcgacctca
     2581 aggtcttccg gctccagcca tctgagctgg cgaagcccgc gctggtcatc gccctcgcgc
     2641 gctactatgc ggaccgccag ccggccatcg gcgccctccg ccactacctc ggctccgtgc
     2701 tactgatggc tccccctgtg gtgctggtat acgcccagcc cgatctggga acctcgtcca
     2761 ccttcgtggt catctggttt ctgatgtcgc tggcggcagg agtacgggtg gtctacctgg
     2821 ggatcaccgc cgtgctgggg ttcgcgtcca tccctgtcat ctggtcgcta ctcaaagagt
     2881 atatgcggga ccgcatcacg atcttcctgc acccggagag cgacccctgg gggcagggct
     2941 acaacatcat ccaagcccag atcagcattg ggtcgggcgg catgacggga agaggtttcc
     3001 ttgccggcac tcagagtcag ggacacttcc tgcgcataca gtactccgac ttcatcttct
     3061 ccgtgctggc agaggaactg gggttcatcg gcgccgtggt gctcttcgcc ctctttcttc
     3121 tcctgctgct gcgcatcctc cggattgcag ggcgcgctcg ggacggtttc gggaggctgc
     3181 tggcggccgg gatcgtggtc atgctgctgt tcagcattac cgtgaacatc gctgccaatc
     3241 ttcggctgat gccggtgaca gggatcccgc tacccttcat tagctacgga ggtagttctc
     3301 tgctcaccaa cctggcctgt atcgggatcg ttcagagcgt attgatgcgc cgcaagcgat
     3361 tccttcccgg ctaggcaggt agtagaaaga aacaataact ttggggacac ccgcgaaggc
     3421 tgcgctcgct cgccttcgcc cccgccaggc gctacgccct ggacccgttt ctttctacga
     3481 cgtacttagt gtcgcgtcaa cggatcgcct gaccgtaggt gcggacctgc gtgcccccta
     3541 ccatgccatg ccaaatgcgt tccctgccat ccacccgcca aatgctgtat aacaaacgct
     3601 agtcccccct gaaacccgcc tctatgataa gatttatctg agaaatgctc ggtgccacgc
     3661 cacgtatggg gtgggcgccg cctctggcag cgaagagctg cgtcgcaacg aagggagaag
     3721 tgcagatggg tttcctctca tcggcgggta cgggtttcct gatccttctg gtactggcta
     3781 tcttcctgtt catgtccgca gtcaaggtgg tccaggaata cgagcgaggg gtcatcttca
     3841 gactggggcg gctgatagga gccaagggac cagggctatt cttcatcatc ccagggatcg
     3901 acaagatgat gaaggttgac ctgcgaacag tgactatgga cgtcccggct caggaagtaa
     3961 ttaccagaga caacgtcacc gtaaaggtca acgctgtcat ctacttcaaa gtggtcgacc
     4021 cgaatcaggc ggtcgtgaag gtgctggatt acattcgagc cacctcgcag atcgcgcaga
     4081 ccaccctcag agctgtcctc ggccaatccg aactggacga actgctcgcc cagcgcgaca
     4141 agatcaatcg ccacctccag cagatcatcg acgagcagac cgagccatgg ggtattaagg
     4201 tcagcgttgt ggagatcaag gacgtggaac tgccacagac gatgcagcgc gcgatggcga
     4261 agcaggctga agcagagcgc gagaagcggg caaaggtcat tcacgcccag ggcgagttcg
     4321 atgcctcggc ccgactggcc gacgctgccg atcaactcaa tcgcacgccc actgcgctgc
     4381 agctgcgcta catgcaaaca ctcacggaga tcgcggtcga gaagaactcc accatcatct
     4441 tcccgttgcc catcgacatc ctgaccggcg tcctaccaaa cctgacaaag atgaacggac
     4501 acgctcctgc cagcgaggtt ccgccggcct ctccaatgga tccgctggag ccgagggagt
     4561 cgcgagagta gtgacttcaa cacaacgctg aagagatggt ccggcagtac cgcttgcctg
     4621 gactgcccca cgcacaacgg ctacatgtag ggcgggatct tgtgtcccgc cctttcattg
     4681 ctcggaaatc gccaggtggt tgtatcca
//
LOCUS       DATALS010000309         7074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_216257, whole genome shotgun sequence.
ACCESSION   DATALS010000309 DATALS010000000
VERSION     DATALS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216257"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1446)
                     /gene="melA"
                     /locus_tag="VHS06_06835"
     CDS             complement(121..1446)
                     /gene="melA"
                     /locus_tag="VHS06_06835"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEX2987869.1"
                     /translation="MRKIAFIGAGSFGFTRGLVRDILTYPALADSTIALMDIDPERLD
                     FVKKAVDRIVAAGKYPAKVIATTSRKEALEGADGVLCTILQGGVEVFRSDIEIPKKYG
                     VDTNIGDTRGPSGIFRALRTIPVMVDIAKDIERYCPNAIFLNYTNPMAMLCRAVQGET
                     KVVTSGLCHSVQGTAMMLAGWIGAPYNEITYHCAGINHQAWYLDFKWNGKDAYPMIRE
                     AVETKPEIYNHEQVRNEMYLHLGYYVTESSGHNSEYVAWFRKRPDLIEKYCKTGTGWN
                     PGEYAYILNHYLAVAETWRENIKKDLEAEVDLKRGHEYAASIFNACFGDGEMFEFNGN
                     VRNFGLIKNLPEGCCVEVPVLASKRGLDPIAVGSLPPQLALLNNITAMIEEMTVDGCL
                     TGDPQKIFHAICYDPLTSAVCSLAEIKSMVNEMFEASKDYLTTFKCFKA"
     gene            complement(1621..2676)
                     /locus_tag="VHS06_06840"
     CDS             complement(1621..2676)
                     /locus_tag="VHS06_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX2987870.1"
                     /translation="MMSFRGRKLATEPIPMNIAQLVGKINEYKGMQQLFRQQSPQVLE
                     SLSEVAAVQSTESSNRIEGIEVPTGKLSALMADRAAPQNRPEGEIAGYRDVLATIHAS
                     HEHIPVTPNTILQFHRDLFRYVPSEGGRWKSVDNTIDDVLPDGTHSVRFRPVGAVATP
                     RAVEELCSHLKQHRQDEDVDPLLLGGAFVFDFLCIHPFRDGNGRLSRLLALLLLYSGG
                     YEVGRYISLERIIEKSKESYYGALAMSSEGWHDAKHSLLPWWEYHLGVILSAYREFER
                     RVGVVQNGRGAKSAMVRESVANMVGDFSVTELMELCPTVSRDMVRTVLERMRDEGVVQ
                     CNGTGRSARWQKVESQL"
     gene            complement(2954..5722)
                     /locus_tag="VHS06_06845"
     CDS             complement(2954..5722)
                     /locus_tag="VHS06_06845"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX2987871.1"
                     /translation="MEAIVILVLIILGLIVAALIVPFMALSKVNTLERRLDAMQHELR
                     RVRDSVAPSVEPPAQPAQEAPTQAARQEPPSSPSRIPPRTLDRVAHPTEEAERPIVAP
                     PTPPQQPRTSAPMPSMQPVSSARPSAPRHTRTAGEWEALIGGNLFNRIGAIALLIGVA
                     FLLKYGFDHNWITTWMLVGSGFALGVGLMAAGDRFHKGGAKVFAQGLMGTGVSILYLT
                     VYASANMYHLIPQLPALGLMCVVTAAAFMQAVRTDAPIVALLGLAGGFMTPVLLGGGG
                     GGGGSALGLLAYMAVLDAGLLAMALKKDSWVALEPLTLAATYIVYFGWHAESYSPQML
                     WTVVPFLTVYWLLFYALDVYRSLKSVSTYTDVRMAVAVASSIAFYGAMYGVIYQRYPQ
                     IMGLVTILIASTYFVTMLIMARRQVDAMAAIPRYAVTALTLLAVGTWVQFHHQEYVLA
                     SLWAIEALALVWCGLRYGMRYVWGCALALFAASLIALLDEGLGAVVPKGSVFVANPRF
                     AAFAVVSAASALAAVLFARSEEGSANVVAACLHYAWCGLVFALIGEEIDDYLPCNAVV
                     QQLATGLGWAVFSLPLVWYGLRKRLMPLAYAGLLVMVAGVVAVAISEVVFGSAKGFVP
                     ILNFRAAAFAAVMLCVLAEQFLISRLAGELEWAAWVGGIAQFVLVALGFELISTEIAS
                     YIDKLDALAVPTCFGMYLNQARALLLGAAWAIYSVFLVWYGLARKQDVFTACGYNVLL
                     AATVAVAGWGYRYVPYDHFTLVTNLRAAAFAILTLGMLAHYALLAARSGELQWSSRAL
                     SVLQVVVGLVVFELITAETWSALSVSDNTGSLRQMGLSVVWVVYSILLMSFGIWRRMM
                     ALRFLALALFEITIIKVFLYDLSSLEPLYRIFSFIGLGLFLLATSYLYQRYKSFIFGD
                     AAGRELEG"
     gene            6042..6968
                     /locus_tag="VHS06_06850"
     CDS             6042..6968
                     /locus_tag="VHS06_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX2987872.1"
                     /translation="MPIRLLPVLVLLALTLPAHAELRNVALASDGALIASSSDYGAQA
                     APIAIDGKWNGPGDPADANRWHSALGRPHPHWLWIRFRQPAEISKFVVRRADLLGYPV
                     DFRVEYEPVGGGAMRTLFEVKDNKMTADLWVFERSFEPVVADGVRLWISRSSNEQYTN
                     WSQISEMEVFGEYVGGARKEQNAKAPVDKLPQPQLKPTAHEGLTVTKGPDEIEFRSKW
                     LRIVASRKSARVNALCWDSLGEGKVDTNLLKPGPNGGARFGLTPIFSNGSEQDDVRVD
                     TNGNVIRYTLSLPGGIAARWADELGKGHRALD"
BASE COUNT         1541 a         2311 c         1974 g         1248 t
ORIGIN      
        1 tccggttttg caatgcaaat tctgaaaatc gcagaaaagt ggttacacgt cagaccactc
       61 gagggccaag cacaagggcc ctccgaagag ggcccccata tcgtttccgc caatcacagg
      121 ctacgccttg aagcacttga aagtcgtcag gtagtcctta ctggcctcga acatctcgtt
      181 caccatagac ttgatctctg ccaggctgca caccgctgag gtcagcggat cgtagcagat
      241 agcgtggaag atcttctgcg ggtcgcccgt caggcagcca tccaccgtca tctcctcgat
      301 catggcggtt atgttgttca gcagcgcgag ctgcggcggc agcgaaccga ccgcgatcgg
      361 gtccaggccc ctcttcgatg caagaaccgg tacctctacg caacagcctt ccggaaggtt
      421 cttgatcagg ccgaagttgc gcacgttgcc gttgaactcg aacatctcac cgtcgccgaa
      481 gcaggcgttg aaaatgctcg ccgcgtactc gtggccacgc ttgaggtcga cttccgcctc
      541 aagatccttc ttgatgttct cgcgccaggt ctcggcaacc gcgaggtaat ggttgagtat
      601 atacgcatac tcgcccgggt tccatccggt gccggtcttg cagtatttct cgatgaggtc
      661 aggtcgcttg cggaaccacg ccacatactc cgagttatgc ccgcttgact cggtaacata
      721 gtaaccgagg tgcaggtaca tctcgttgcg gacctgctcg tggttgtaga tctcaggttt
      781 ggtctctacg gcctcgcgga tcatcgggta ggcatccttg ccgttccact tgaaatccag
      841 ataccacgcc tggtgattga taccggcgca atggtaggtg atctcgttat aaggcgcgcc
      901 tatccaaccc gccagcatca tcgcagtacc ctgcacgctg tggcagaggc cgctggtgac
      961 taccttggtc tcgccctgca ccgcgcggca cagcatcgcc atcgggttgg tgtagttcag
     1021 gaagatggcg ttggggcagt agcgctcgat atctttggcg atatcgacca tcacgggaat
     1081 ggtgcgcaga gcccggaaga tgcccgacgg cccgcgggtg tcgccgatgt tggtatccac
     1141 cccatacttc ttgggaatct caatgtccga gcggaatacc tccaccccac cctgcagaat
     1201 ggtgcacaga acgccgtccg cgccctcgag cgcttccttg cggctggtgg tggcgataac
     1261 ctttgcaggg tacttgcccg cagccacaat ccggtccaca gccttcttca cgaaatcgag
     1321 gcgctccgga tctatgtcca taagcgcgat ggtgctatct gcgagagccg gataggtgag
     1381 aatgtctctc acaagccccc tggtgaagcc gaagctgccg gctccgatga atgcgatctt
     1441 tcgcatgact gatctccttg tgtgatttga aagctccaag ctttgcgttc ggcactacga
     1501 cgcgggtatc ctgcaccaaa gcagatgctc aatggtaaat gatcagcgct ccataacgga
     1561 tgcgcaaacg tggtaatatg gagggcacaa cgtggtaatg aacgcgccct attaccaagt
     1621 ttatagctgc gactccactt tctgccacct ggcagagcga cccgtgccat tgcactgaac
     1681 gaccccctcg tcccgcatcc tctcaagcac cgtcctgacc atgtccctgc taacagtcgg
     1741 acacagctcc attagctcag tgaccgagaa atcaccaacc atattggcca cggattcgcg
     1801 caccatggcg gacttagccc ctcgcccatt ctgcacgacg ccgacgcggc gctcgaactc
     1861 cctataggcg gacagtatca ctcccaggtg atactcccac cagggcagca gagagtgctt
     1921 ggcatcgtgc caaccctcag acgacatcgc cagcgccccg tagtacgact ccttgctctt
     1981 ctcgatgatg cgctccaagc ttatgtaacg cccgacttca tacccccccg agtagagcag
     2041 aagcagcgcc agcagccgtg acagccggcc gttgccgtcg cggaagggat ggatgcacag
     2101 aaagtcgaag acgaacgccc ctcccaggag cagcgggtcc acgtcctcat cctggcggtg
     2161 ctgcttcaga tgcgaacaca gctcctccac tgcgcgcggt gtggcaacgg cgccgacggg
     2221 gcggaagcgc acgctatgag tgccgtccgg caacacatcg tctatcgtgt tatcaactga
     2281 cttccatctg ccgccttcgg acggcacata cctgaacaag tcgcgatgaa actggagaat
     2341 ggtgttcggc gtcaccggga tgtgctcatg cgaggcgtga atcgttgcga gcacgtcccg
     2401 gtacccggca atctcgccct cgggccgatt ctgcggggca gcccggtccg ccatcagagc
     2461 actaagcttg ccggtaggga cctcgatgcc ctcgattcgg ttcgaggact cggtgctctg
     2521 caccgccgcc acctcgctca gcgactccag gacctgcggc gactgctgcc ggaagagctg
     2581 ctgcatcccc ttatactcgt tgatcttgcc cacaagctgg gcaatgttca tagggatcgg
     2641 ctccgtcgcc agcttacgac ctctgaatga catcatcctg gtaaacctct gcccggacaa
     2701 acgtggtagt actaacacta aacgtggtaa tacgagctac atattaccac gtttagcaaa
     2761 cgagaacaac cagaccagcc ctcgcgtgtc tgttcattac tgcgggagcg aaccccgcta
     2821 gcactgagta ggcccgagga acgagggccg tatcgaagtg cgggctgata tccgactgcc
     2881 cctcgataca ccgactgcgt cggcactcgg ggctaacgga gtcaggttcc cgcagaactg
     2941 aactcactcg ccctcacccc tccagctcgc gcccggcagc gtcaccgaag atgaagctct
     3001 tgtatctctg atacaggtac gaggtcgcca gcaggaatag gccaaggccg atgaatgaga
     3061 atatccggta gagcggctcc agcgacgaca ggtcatagag aaacaccttg atgatcgtga
     3121 tctcgaacag agccagagcc agaaacctca gcgccatcat cctccgccag atgccaaaac
     3181 tcatcagcag tatcgaatag accacccaca ccaccgacag ccccatctgc cgcaggctgc
     3241 ccgtattgtc cgagacgctc agcgcgctcc aggtctcggc tgtgatcagc tcgaacacaa
     3301 ccagacccac cacaacctga agaacgctga gtgctctgga gctccactgc agctcccccg
     3361 aacgcgccgc cagcagcgca tagtgcgcca gcattccaag cgtgagtatg gcgaacgccg
     3421 cggcgcgcag attcgtgacg agggtgaagt ggtcgtaggg cacgtatctg tatccccacc
     3481 ccgccacggc gaccgtcgcc gcgaggagca cgttgtagcc gcaggcggtg aacacgtcct
     3541 gcttccgcgc cagaccatac cataccagga acaccgaata gatcgcccac gccgcaccca
     3601 gcagaagcgc tctggcctgg ttcagataca tgccaaagca ggtgggaacc gccagggcgt
     3661 ccagcttgtc tatgtatgac gctatctcag tagatatcag ctcaaagcca agggcgacca
     3721 gcacgaactg cgctatgccc ccaacccagg ctgcccactc cagctcgccg gcgagcctgc
     3781 ttatcaggaa ctgctccgcc aacacacaca gcatcaccgc cgcgaacgcc gccgcccgga
     3841 agttcagaat cggcacaaac cccttggctg atccaaacac tacttcgctg atcgccacgg
     3901 caaccacccc cgcaaccatc accagcaatc cggcataggc cagcggcatc aggcgtttcc
     3961 tcagaccata ccaaaccaac ggcagcgaga acacagccca tccaaggccg gtggccagtt
     4021 gctgaacgac cgcgttgcac ggcagatagt cgtcgatctc ctcgccgatc agagcaaaca
     4081 ccaagccgca ccacgcatag tgcaagcacg cagcgaccac gttcgcagag ccctcttcag
     4141 accgggcgaa aagcacagcc gccagcgcgg acgcggcaga cacaacggcg aacgccgcga
     4201 agcgaggatt agctacgaaa accgagccct tgggcacaac agcgcccagg ccttcgtcaa
     4261 gcaatgcgat cagagacgcc gcgaataggg cgagagcgca tccccacacg tatcgcattc
     4321 cataccttag cccgcaccat accagcgcca gagcctcgat ggcccacagg ctcgcaagca
     4381 catactcctg atggtggaac tgcacccacg tcccgacagc cagcagcgtg agcgccgtca
     4441 ctgcgtagcg cggtatcgcc gccatggcat caacctgtcg ccgggccatt atgagcatag
     4501 taacgaagta ggtggacgcg atgagtatcg tgaccaaccc catgatctgt gggtagcgct
     4561 ggtagatcac gccgtacatc gcgccgtaga acgcaatcga actggcgacc gcaacagcca
     4621 ttctcacatc cgtgtatgtc gacactgact tcaggctccg atagacatcc agagcataga
     4681 acagcagcca gtacacagta agaaacggca ccaccgtcca cagcatctgc ggcgagtaag
     4741 actcggcgtg ccagccgaag taaacgatgt aggtagccgc cagcgtcagc ggctcgagtg
     4801 ctacccatga gtccttcttc agcgccatcg cgagaagccc agcatcgagc accgccatat
     4861 aggcaagcag ccccagtgcg ctgcctccgc cgccaccacc gccacccagc aggaccggcg
     4921 tcatgaaccc acccgccagc ccgaggagcg caacgatagg cgcatcggtg cgaacagcct
     4981 gcatgaacgc cgccgccgtg accacgcaca tcagcccgag cgcgggcagt tgcggtatga
     5041 gatgatacat attggcggag gcgtagacgg tgaggtatag aatcgacacc cccgttccca
     5101 tcaacccctg cgcgaacacc ttcgccccac ccttgtggaa ccgatccccg gcagccatca
     5161 ggcccacgcc tagcgcaaat cccgacccca ccagcatcca cgtggtgatc caattgtggt
     5221 cgaagccata tttgagcagg aaagcaacac cgatcaggag cgcaatcgcc ccgatgcggt
     5281 tgaacagatt cccgccgatc agagcctccc actctcctgc tgtgcgtgtg tgacgcggtg
     5341 cggatggcct ggcggacgac accggctgca tcgatggcat cggagctgag gtacgaggct
     5401 gctgcggcgg agtcggcgga gcaacaatcg gcctctcggc ctcctcggtc ggatgcgcca
     5461 cccgatccag tgttcgcggc ggtatccggc ttggcgaaga gggcggctcc tgccgagctg
     5521 cctgagtcgg cgcctcctgc gcgggctgtg cggggggctc cacggacgga gcaacgctgt
     5581 cacgcacccg ccgaagctcg tgctgcatgg catctagccg cctctccaag gtattcacct
     5641 tggacagagc catgaacgga acgatgagag cggccacgat caacccaagt ataatcagca
     5701 ccagaatgac gatggcttcc acgagcgcct ccaccacccg tttgcctcaa ggctgccggg
     5761 ctgctgttga gtttacgctg cgcaagcacg gctgtcaact caagacccag cttggtgtct
     5821 gttcagtctt gcgggagtga tccgctagtg ctgagtggtc cgcaggaccg tatcgaagca
     5881 cgggctgcat tcgaccgccc ctcgatacac cgactggcgt cggcactcgg ggctagcgga
     5941 gtcaggttcc cgcagaactg aactcacacc cagcttgacg ccaagccgag ccgttggtat
     6001 catccgccat atcacagacc tatggaatgg tgacaaccga gatgcctatc agactcctgc
     6061 ccgttctcgt tctccttgcc cttaccctgc cggcccacgc cgagctccgc aacgttgccc
     6121 ttgcaagcga tggcgcactg atcgcctcaa gctccgacta cggcgcacag gccgcgccca
     6181 tagccattga cggcaaatgg aacggccccg gcgacccggc cgatgccaac cgctggcaca
     6241 gcgccctcgg caggccgcac ccgcactggc tatggattcg cttccgccag ccggctgaga
     6301 tatccaagtt cgtagtacgc agagccgatc tgctcgggta tccggtggat ttcagggtcg
     6361 agtatgaacc ggtcggcggc ggagctatgc gcacgctgtt cgaggtcaaa gacaacaaga
     6421 tgaccgccga tctgtgggtg ttcgagcggt cgtttgagcc ggtggtggcg gacggcgtcc
     6481 ggttgtggat ctcccgatcc tcaaatgagc agtatacgaa ctggagccag atatcggaga
     6541 tggaggtgtt cggcgagtac gtgggcggcg ctcgaaagga gcagaacgcc aaagcgcccg
     6601 tcgataagct gccccaaccc caactcaagc cgaccgcgca cgaaggtctg acagtaacca
     6661 aaggccccga cgagatcgag ttccggtcga aatggctgag aatcgtggca tcgcgcaagt
     6721 cggcgcgggt caatgcgctc tgctgggaca gcctcggcga gggcaaggtg gacacaaacc
     6781 tgctcaagcc cggcccaaac ggcggagcga ggttcgggct gacgccgatc ttctcaaacg
     6841 ggtccgagca agatgacgtg cgagtcgaca ccaacggaaa cgtcatccga tacaccctct
     6901 ccctccccgg aggaatcgcc gcccgctggg ccgatgagct tggcaagggg catcgcgctc
     6961 tcgactgaga gcgttaggtc cagccccgac ttgcccggcg cgagggcgaa gagcccgtcc
     7021 tcgccacgcg gagcgttgtc gatgaggttc gcattccact gcgccagcca gcgg
//
LOCUS       DATALS010000310         7250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_216456, whole genome shotgun sequence.
ACCESSION   DATALS010000310 DATALS010000000
VERSION     DATALS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216456"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VHS06_06855"
     CDS             <1..849
                     /locus_tag="VHS06_06855"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HEX2987873.1"
                     /translation="MMLASAFVHPEREGIIASRKGLLVMNLVGSAVPLLLFTYWSQAL
                     PLLASPDGELTPLKYGLEYVIIGGMLAGAVAHYANPIGVRDEPRGMVVAALVVSALGE
                     MPFTVGVTSFNIHLLVGHLLKALSYYLVFRALLVTSVRRPYVQLRIANDSLEETVSEL
                     DSRNRELDALVDVAITLTGSLNPSQVLRSAIDKATKVMGARAGAIFLRDEDSNRLRLV
                     AWEGLPQEMVDQFVAVPPMLRTSFEAPGAGGGTSALDDKAVLRSLGGGRRQDRTAGGL
                     PLQPYR"
     gene            878..2710
                     /locus_tag="VHS06_06860"
     CDS             878..2710
                     /locus_tag="VHS06_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2987874.1"
                     /translation="MTAEDGRRFTLADADLLTAISFQLGLAIENARLYERTDERLREK
                     LEELSTAERRSRFLAEVGVLLGSSMDADTVMEMVASKTTEMVGDACAIYLIDGENDRL
                     LRLKALANAVAGDDEVESVKELLVRRPLEVGRGAIGLVAQDGQPHLFVQVDREQLARE
                     NRYLAQSVEDIAVLSKVAPKSRIAAPLRAHGRTNGVLAIYSFAESEPLQEPDLALVTE
                     LADRLGVAVENMRLFHESQARRLRLEAIIGQMADGVVVTNEVGEAVVVNVAAEKMLGG
                     LIAPSSGMKPQNGCKSKKGTISPSIRALVMRALAGEMILGEEFNVGAPPLRRVLNATA
                     SPVRDDDREITGAVLVLRDVTVEREVDRMKEEFVATVSHELRTPITAVLGYTDILLRG
                     LRGPLEPKQVDALSSVRTAGERLLALINDLLDISRLEAGAQDMSIRPLDLAESINRAI
                     EAFSGLALSREIEMLQAIPVSLPLVQGDEELLQRILGNLLSNALKFTPEGGTVVVSAM
                     AGMPSHANARGAQRRQDHGQDVVSVMVTDTGIGVPREQQEKIWEKFQQADSSSRRSFG
                     GTGLGLAIAKGLVELHGGTVWLESEGLPGKGSTFGFTLRVAKSA"
     gene            complement(2688..3551)
                     /locus_tag="VHS06_06865"
     CDS             complement(2688..3551)
                     /locus_tag="VHS06_06865"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX2987875.1"
                     /translation="MRIGAHVSTSGGVHTAIGRAEEIGANCVQIFAGAPQRWAEARFP
                     DADVAAFRRLAEEKDVRPAFIHSSYLLNFASADETLREKAVISLASGLGWADRLGAAG
                     VITHLGSSKEGDPNAAVEQVGRELSRVLAASPTGANLLMETCAGQGNTIGRRFEQIGA
                     LVRMLDGDSRVQVCVDTAHIFEAGYDISSESGLAATLEEFDRHIGLSRLSAIHINDSK
                     TALGSNVDRHENIGFGMIGEEGLGRVLQHPVLSGIPFLLEVPGMEKQGPDLPNVNALR
                     RLAGLKPISRP"
     gene            3820..4887
                     /locus_tag="VHS06_06870"
     CDS             3820..4887
                     /locus_tag="VHS06_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987876.1"
                     /translation="MVSQTAAKVADSIASKPASQESVRGFLALSSRSAQLRDDLNSGN
                     PRDPAAESEELAEIDRQMSSEEAGVERLIQDQVSAVLARSGVGYRLPGSAGLFPPVVF
                     RYTPLPRLLVVSPRDRIDRVASVLLDPRTAPDEVEELEARVSKKGYIGLVVPIGGLGV
                     FPSMVPDGTDLRNSFIAVAHEWTHHYLALRPLGWKYAVGSESDQRMVTVNETVAEIVG
                     QQIGDEVYRDIYRGAEGETQAQSSSGRTDRFRSEMRAIRLEVDRLLAEGKIGDAEAFM
                     ASSRDRLQAEGFYIRKLNQAYFAFYGSYAAEPSLSGPQGERISERVHALRDGSPTLGA
                     FLSRVSEVGSYQAFSQLSVSR"
     gene            4969..5544
                     /locus_tag="VHS06_06875"
     CDS             4969..5544
                     /locus_tag="VHS06_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX2987877.1"
                     /translation="MDPNREQAMELLTEFTKSDSLIKHALAVEAAMRAYARRFGEDEE
                     KWGVVGLIHDFDYEQNQSWEQHVYVGARILRERGWPEEYVQGMESHANYMNVPRDTNM
                     RKSLFAVDELSGFISAVALVRPSKSIMDVEASSVKKKMKDKAFARSVSREDILEGAKE
                     LGIPLEEHISFVIEAMKPVAKDLGLDGSAAR"
     gene            5818..6063
                     /locus_tag="VHS06_06880"
     CDS             5818..6063
                     /locus_tag="VHS06_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987878.1"
                     /translation="MAKLRILSHQGDTSVEWDVSRAEVGDPEALEAIREAERIFEENR
                     AKGATAFKVEPGKPAEKLERFDQTAEQIIMVPRIAGG"
     gene            6060..6557
                     /locus_tag="VHS06_06885"
     CDS             6060..6557
                     /locus_tag="VHS06_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987879.1"
                     /translation="MIDAVGSTLHWELAAWVGLAILAVQFVGLVFWVMLGSRGRVDVG
                     TGHSLQLEAERRAEALLQEVLPEEQNRDLDSRGYLEVPSPSRQNRVYRVPRGRGMVSV
                     YEDGRAIMSLCVQPVEWVPGADVVLMHKLMIEGNEEDYLRIANQFEPGYPYYLRWDRR
                     TGRPA"
     gene            complement(6659..>7250)
                     /locus_tag="VHS06_06890"
     CDS             complement(6659..>7250)
                     /locus_tag="VHS06_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX2987880.1"
                     /translation="IPLPSEIIMPLAGWMVYRGVFDFWIAVIAGTVGNTLGSTIAYWV
                     GSIGGRPIIAKYGRYVLISEHDLDIADRWFAKYGHSAVLFSRLLPVVRTFISFPAGVT
                     RMNFGKFVLYSTLGALPWVAALCYAGKLMGDNWVQVRGVLHNLDYPILAVLIALVGYY
                     VYRHMKPKKKHSEKMKAAEIVKPKSKELYHSGRNQD"
BASE COUNT         1393 a         2035 c         2425 g         1397 t
ORIGIN      
        1 atgatgctcg catccgcctt cgtccaccct gagagggagg gcatcatcgc tagccggaag
       61 gggttgctcg tgatgaacct tgttggttcc gcggtccccc ttctgctttt tacctactgg
      121 tcgcaggctc ttcctcttct agcgtcaccg gatggcgagt tgactccgct gaaatatgga
      181 ctggagtacg tgatcatcgg ggggatgctg gccggcgccg tcgcgcatta cgcgaatccg
      241 attggggtca gggatgaacc gcgggggatg gtcgtggcgg cgctggtggt gagcgcactg
      301 ggagagatgc ccttcacggt gggagtgacc tctttcaaca tacacctgct ggttgggcat
      361 ctgctgaagg cattgagcta ctacctcgtg ttccgggcac tcctcgtaac gtcggttcgc
      421 aggccttacg tgcaactccg catcgcgaac gatagcctcg aggagacggt ctccgagttg
      481 gacagccgca accgtgaatt ggatgccctg gtggatgttg ccatcaccct taccggcagc
      541 ctcaacccat cgcaggtgct gcgctcagct atcgataagg ctacgaaggt catgggcgcc
      601 agagcggggg cgatattcct gagggatgag gactccaacc gactccggct ggttgcctgg
      661 gaagggctgc cacaggagat ggtagaccag ttcgtcgcgg tgccccctat gctacgcacc
      721 agcttcgagg cccccggggc tggtggtggt accagcgcac tggatgacaa ggcggtcttg
      781 cggagtcttg gggggggccg acgccaggat cgcaccgctg gggggctgcc tctgcagccc
      841 tatcgctagc aagggccgcg ttcgcggcgt cgtagcgatg accgccgagg atgggcggcg
      901 cttcaccctg gcggatgccg acctgctgac tgcgatcagc ttccagcttg gcctggctat
      961 cgagaacgcc cgtctttacg agaggacgga cgagagactg agggagaagc tcgaggagtt
     1021 gagcactgcg gagcgcaggt cgcgcttcct tgccgaggta ggagtactcc tggggagttc
     1081 gatggacgca gatacggtga tggagatggt ggcgagcaag accacggaga tggtcggcga
     1141 cgcatgcgcc atctacctca tcgacggcga gaacgaccgg cttcttcggt taaaggcgct
     1201 tgccaacgca gtcgccggtg acgatgaggt cgagtccgtg aaggagttgc tggtccgtcg
     1261 accgctggag gtaggacgcg gagcgatcgg attggtggcg caggacggtc agcctcatct
     1321 gttcgtgcag gtcgatcgcg agcagttggc tcgcgagaac cgttacctgg cccagtctgt
     1381 ggaggacata gccgtcctga gcaaggtagc tcccaagtct cggattgcgg caccccttcg
     1441 agcccatggc aggaccaatg gggttctcgc catctactcc ttcgccgagt cggagccttt
     1501 gcaggaaccg gatctggccc tcgtgacgga gttggcggac cggctgggag tggccgtgga
     1561 gaatatgcgg ttattccatg agagtcaggc gaggagactg cgcctggagg cgatcatcgg
     1621 tcagatggcg gatggagtgg tggtgacgaa tgaggtcgga gaggcagtgg tggtcaatgt
     1681 cgctgccgag aagatgctcg gggggctgat cgctccctcc tcgggtatga agccgcagaa
     1741 cggctgcaag agtaagaagg ggacgatctc accctctatc cgtgcactgg tgatgcgagc
     1801 gctagcgggg gagatgatcc tgggggagga gttcaacgtc ggcgcgccgc ctttgcggcg
     1861 tgtcctcaac gctacagcga gccctgtccg cgacgacgac cgagagataa ccggtgctgt
     1921 gctagtgctg cgggacgtga ccgttgagcg tgaggtggac aggatgaagg aggagttcgt
     1981 ggctaccgtc tcccacgagc tacggactcc cattaccgcc gtccttggct atacggatat
     2041 cttgctccgg gggctgcgag gtccgctgga gccgaagcag gttgacgcgc tctcgtccgt
     2101 tcgcaccgca ggggagcggc tactggcact gatcaacgac cttctcgaca tatcgcggct
     2161 ggaggcgggg gcccaggata tgtcgatccg gccgcttgac ctggcggagt ccatcaaccg
     2221 ggctatcgag gcgttttcgg ggctcgctct gtcgagggag atcgagatgc tgcaggcgat
     2281 tccggtttcc ctaccgctgg tgcaggggga cgaggaactg cttcagcgaa tcctcggcaa
     2341 cctgttgtcc aacgcactga agttcacccc ggagggggga accgtggtag tgtccgcgat
     2401 ggctggcatg ccgagccatg ccaacgctcg gggcgcacag cgcaggcagg accacggaca
     2461 ggatgtggtg tcggtcatgg tgacagacac cggaatcggc gtgccaaggg agcagcagga
     2521 gaagatctgg gagaagttcc agcaggcgga ttcctcctcc cgacgctcct ttggagggac
     2581 cggtctgggt ctggcgatag cgaaggggct cgtggagctg catggcggca ccgtgtggct
     2641 cgagagcgag gggctgcccg gcaagggcag caccttcggt ttcaccctca gggtcgcgaa
     2701 atcggcttga ggccggcgag tctgcgaagt gcgttaacgt tgggcaggtc gggcccctgc
     2761 ttctccatgc caggtacctc gagaaggaac gggatgccgc tcagcactgg gtgttgaagt
     2821 accctcccca atccctcctc cccaatcatt ccgaacccga tgttctcatg tctatctacg
     2881 ttggatccga gggcggtctt ggagtcgttg atgtggatcg cgctcagcct cgacagcccg
     2941 atatgccggt cgaactcctc cagcgttgca gccagcccgg attcggagct gatatcgtag
     3001 cctgcctcga agatgtgggc ggtatccaca caaacctgca cacggctgtc gccatccagc
     3061 atcctgacga gggcaccaat ctgctcgaag cgtcggccga tagtattccc ctggccggca
     3121 caggtctcca ttaggaggtt ggccccggtt ggcgacgcgg ccagcactct ggacagctct
     3181 ctccctacct gctcgacggc tgcattcgga tctccctcct tgctgctgcc caggtgggtt
     3241 atcacgcccg ccgctcccag tctatcagcc catcccagtc cggaagcgag cgagatgact
     3301 gccttctctc gcagagtctc gtctgccgag gcgaagttca gaagatagga gctgtggatg
     3361 aaggcggggc ggacatcctt ctcctctgcg agccgcctga aggccgcaac atccgcatca
     3421 gggaagcggg cctcggccca gcgctgcgga gcacccgcga atatctggac acagtttgcc
     3481 ccgatctcct ctgctcgacc aatcgccgtg tgcacaccgc cgctggttga tacgtgcgcg
     3541 cctatcctca tcgacattac ctcttgtctg catctgctgg cggcgatttt agcagagcga
     3601 gtcggccatg tccctagcgg aacaatcatg atcctccggc cataggcggc ggcacccggg
     3661 ctgcggtata gtcttcacat gagagtccga accattctgc tgctattcgt tctgtctctg
     3721 cttcccgttg tcctcgggat cgcagggggg agttcggatt ctgtcgcttt ccgcttcgat
     3781 agactctcat atccgtatgt ggagagcgtt actgggtgga tggtgagcca gactgccgcg
     3841 aaggtggcgg actcgatagc atcaaagccg gcctctcagg agagcgtccg cggtttcctc
     3901 gccctctcca gccgaagcgc acagctcagg gacgacctga acagcggaaa tcctcgtgat
     3961 ccggccgcag aatcggagga gttggctgag atcgacaggc agatgtcttc ggaggaagcg
     4021 ggagtcgagc ggctgataca ggaccaggtc tccgccgttc tcgcgcgctc cggtgttggt
     4081 tatcgactcc ccggcagtgc cggcctcttt cctcccgtgg tcttcagata cacaccgttg
     4141 ccgaggctgc tggtcgtttc ccccagagac aggatagaca gggtcgcatc ggtgctgctg
     4201 gatccacgca ctgcccctga tgaggtggag gaactggagg ccagggtgtc aaagaagggc
     4261 tacatagggc tcgtggttcc tatcgggggg ctgggcgtat tccccagtat ggtgcctgac
     4321 ggcacagatc tgcgcaacag ctttatcgcc gtggctcacg agtggaccca ccactacctg
     4381 gcgctgaggc cgcttggatg gaagtacgcc gtgggctcgg agtccgatca gcgcatggtg
     4441 acggtcaacg agaccgtggc cgagatcgtg ggccagcaga taggagacga ggtctacaga
     4501 gacatatacc ggggggcgga gggagagact caggcgcagt cctccagcgg acggacggac
     4561 cggttccgta gcgagatgcg cgccatcagg ctggaggtgg ataggctgct ggccgagggg
     4621 aagatcggcg acgctgaggc gttcatggcg tcgtcccgag acaggctcca ggccgagggc
     4681 ttttacatcc ggaagctgaa ccaggcgtac ttcgctttct acgggagcta tgccgcggag
     4741 ccgtctctct cagggcccca gggcgagcgg atcagcgaga gggtacacgc gctgagagac
     4801 ggatccccga ccctgggggc cttcctctcg cgtgtgtccg aggtcgggag ctatcaggcg
     4861 ttctcgcagc tcagcgtctc tcgttgaggt agggctataa ttggcgcagc acgggccgcc
     4921 gcgacggata gaacggcgga acccctcgat atgttggtgg aggtcggtat ggatcccaac
     4981 cgagagcagg cgatggaact gctcaccgag tttaccaaga gcgacagtct gatcaagcat
     5041 gcactcgcgg tagaagccgc gatgcgggcc tacgcccgcc gcttcgggga ggacgaggag
     5101 aagtggggtg tggtcggctt gatccacgac ttcgattacg agcagaacca gagctgggag
     5161 cagcacgtct acgtcggggc caggatactg agggagcgcg gctggcccga ggagtacgtc
     5221 cagggcatgg agtcgcacgc caactacatg aacgtaccgc gtgacaccaa catgcgcaag
     5281 agcctgttcg ccgtggacga actctctggt tttatctcgg ccgtggcgct agtgaggccg
     5341 agcaagagca tcatggatgt ggaagcatct tcggtaaaga agaagatgaa agacaaggct
     5401 ttcgcgcgat cggtctcgag ggaagacatc ctggaagggg ccaaggagct tggcatccca
     5461 ctggaggagc acatctcctt tgtcatagag gctatgaagc ctgtagcgaa ggaccttggc
     5521 ttggacggtt cggccgccag gtagttagcc cgctatcctc tccatcaata tgcccggatg
     5581 caatgcccgc ggacttcgcg ggcatttctc ttgcatagcc tacgttagct acggtgggcc
     5641 ggatgagcct ggcagcagcg actcaagcag gtggaggttg gtacgcattc tgcgacgcct
     5701 tcctgctcct acgagcgcga acctcagtaa tgagggagcg gccgtgggtg gttgatccca
     5761 tcccgaaatc gagccggatt tcgccaggga acacttggag aggagacgga accgataatg
     5821 gcgaagctga ggatactaag ccatcaaggc gacaccagcg tagagtggga cgtcagccgg
     5881 gcggaagtgg gcgatcccga ggcgctggaa gcgattcgag aggctgagag aatcttcgag
     5941 gagaacaggg ccaaaggtgc aacggccttc aaggttgagc ccggcaagcc tgctgaaaag
     6001 ctcgagaggt tcgatcagac agccgaacag atcatcatgg ttcccaggat cgcaggcgga
     6061 tgatcgacgc agtgggctct actctccact gggaactggc tgcctgggta gggcttgcca
     6121 tcctggcggt ccagttcgtc ggtctggtct tctgggtcat gctgggctcg cgggggcggg
     6181 tggacgtcgg cactggccac agtctgcagt tggaggccga acggcgagcc gaagcactgc
     6241 tgcaggaggt gctgcctgag gagcagaaca gagacctgga ctctcgcggc tatctggagg
     6301 tgccaagccc gagccggcag aatagagtgt accgtgtgcc caggggacgc ggcatggtga
     6361 gtgtctacga ggacggacgg gcaatcatga gcctgtgcgt tcagccagtg gagtgggtcc
     6421 caggtgccga cgtcgtcttg atgcacaaac tgatgattga gggaaacgaa gaggactatc
     6481 tcaggatagc caatcaattc gagcccggat atccctacta cctccggtgg gatcgccgga
     6541 ccgggaggcc cgcgtagacc tccatagatg acagactgcg cagggacgaa agccccccgt
     6601 cgtcgatctc ctaatggtcg acgacggggg gcttctcttt gggtcctttg tcgagagtct
     6661 agtcctggtt tcggccggag tggtaaagct cctttgactt gggcttcacg atctccgcag
     6721 ccttcatctt ttccgagtgc ttcttcttcg gcttcatgtg gcggtagacg tagtaaccga
     6781 ccagcgcgat aagcacggcg aggatagggt aatcgaggtt gtgaagtaca ccccgaacct
     6841 gtacccaatt atctcccatc agtttgcccg cgtagcagag ggctgccacc cacggtaaag
     6901 cgccgagggt ggagtagagg acgaacttgc cgaagttcat ccgcgtcacg ccagcgggga
     6961 aggagatgaa ggtgcgtacc acaggcagca acctggagaa cagtacggcg ctgtggccgt
     7021 acttcgcgaa ccagcgatcg gcgatatcca ggtcatgctc ggagatgagg acgtagcgtc
     7081 cgtacttggc gatgatgggt cggcctccaa tcgaccccac ccagtaggcg atggtcgatc
     7141 ccagggtatt acctacggtt ccggctatga cggcaatcca gaaatcgaag actccgcggt
     7201 acaccatcca gccggctagc ggcatgatga tctcgctggg tagggggatg
//
LOCUS       DATALS010000311         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_33295,
            whole genome shotgun sequence.
ACCESSION   DATALS010000311 DATALS010000000
VERSION     DATALS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33295"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VHS06_06895"
     CDS             <1..377
                     /locus_tag="VHS06_06895"
                     /inference="COORDINATES: protein motif:HMM:NF025210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX2987881.1"
                     /translation="PLAEAGISVDGIVQNVTVEGATALSFTVARGDLPKAMRIIEPVA
                     QRIGAKQLLSSDNQGKVSVVGAGIQSAPGVAAATFQALFEAGINIEMITTSEIRITCI
                     VDRERVQEAVQKLHSTFELDQA"
     gene            complement(390..641)
                     /locus_tag="VHS06_06900"
     CDS             complement(390..641)
                     /locus_tag="VHS06_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987882.1"
                     /translation="MAARALQAAIYLANVLLGISILALLLGLVLAARPELFAGFGDSA
                     SSRTGLAIALAGGFGAILCTVMAHLLELIAGSVDNGDKV"
     gene            complement(784..2226)
                     /locus_tag="VHS06_06905"
     CDS             complement(784..2226)
                     /locus_tag="VHS06_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2987883.1"
                     /translation="MRQSAPVEVELTQRIHWLIQLRWLAATTVLYGTFLATTVAGIRL
                     APLPLYLIGAAIAMYNALFIIGLKWTEEASKALRLDHAKGMAHGQIVTDLVFLTLLIH
                     FTGGVENPLSFYFIFHVIIASILLSRWETFVQSTVAIFLFGALVLGEYAGAIPHITLF
                     GPVRPDLATDGQFVAVALLVFSSTIYLSAYMATSIVKRLRERDQEILQLTQELQQKAR
                     ELEVAYNHLAELEQLKSLHMGRMSQEMKAPLTAIQSSLKGVLEGLTGEVSATQREMVS
                     RAETRTRALLDLVTDLLILSRAREAKLLTDRRAVSPREIVEKVTGIENSRALSKDVTL
                     SAQFSEDVPTMYADPETIKQLVHNLVANAVTYTLSGGRVELKVDSIGEAIRFRISDSG
                     IGIPAAEIPRIFNEFYRAENARRYSDEGTGLGLSIVRSTVDARGGDIKVESQVGTGTT
                     FTVILPVGKAPDDEGEGAVEGNQSETRTLP"
     gene            complement(2424..>2836)
                     /gene="hypB"
                     /locus_tag="VHS06_06910"
     CDS             complement(2424..>2836)
                     /gene="hypB"
                     /locus_tag="VHS06_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009378576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX2987884.1"
                     /translation="INTGGDCHLNANMVRSALEDLPLEEIDLLFIENVGNLVCPAGFD
                     IGEEIKVVILSVAEGDDKPEKYPLIFQQSSAMVLNKLDLTGMVDFDFERARSAARATN
                     RELPIFELSARTGRGMEGWLEWLRSRVKSGMVNS"
BASE COUNT          534 a          896 c          865 g          541 t
ORIGIN      
        1 agccgctggc ggaagcgggg ataagcgtcg acgggatcgt gcagaatgtc accgtcgagg
       61 gtgccacggc cctctccttc accgtggcgc gaggcgattt gcctaaggcg atgcggatca
      121 tcgagccggt ggcgcagcgg attggggcga agcagctgct cagctccgac aaccagggga
      181 aggtgtcggt cgtgggggcc ggcatccaga gcgcgccggg cgtcgcggcg gcaaccttcc
      241 aggcgctgtt cgaggcgggg atcaacatcg agatgatcac cacctcggag attcggataa
      301 cctgcattgt ggatagggaa agggtgcagg aggcggtgca gaagctgcac tccaccttcg
      361 agctggacca ggcgtaggta tactaattcc tagaccttgt cgccgttgtc caccgagccc
      421 gctatcaact ccagcagatg ggccatcacc gtgcagagga tggccccgaa gcctccggcg
      481 agggcgatgg ctagaccagt tcgcgaggaa gccgaatcac cgaagccggc gaaaagctcc
      541 ggcctggcgg ccagcaccag ccctagaagc agggcgagga tggagatccc cagcaggacg
      601 tttgccaggt atatcgctgc ctggagcgcg cgagccgcca tgtagccctc ctgccccaag
      661 tcccgggcag cccaatagca gcatccacaa ccactctatc cagcaagggg cgtacctgtc
      721 cggttgggac cctcctagcg ccagatggac aggatggcga cgccggttct gctctcctga
      781 tccttatggc agtgtcctgg tttcagactg gttcccttcc acggcgccct ccccctcatc
      841 gtcaggagcc ttgccgacag ggaggatgac ggtgaaggta gtgccggttc ccacctggct
      901 ctccaccttg atatcaccac cccgagcatc caccgtgctc cgcacgatgg acaacccgag
      961 gcctgtgccc tcgtcgctgt accttcgggc gttctcggct cggtagaact cgttgaagat
     1021 ccttgggatt tcggcggcgg gaatgccgat gccgctatcg ctgatgcgga agcggatagc
     1081 ctctccaatg gagtccacct tgagctccac tctcccccca ctgagagtgt aggtcacggc
     1141 gttggccacc aggttgtgga ccaactgctt tatggtttcc ggatcggcat acatggtggg
     1201 aacgtcttcc gagaactgcg cggatagggt gacatccttg gacaacgccc gcgagttctc
     1261 gatccctgtc accttctcca caatctctcg cgggctcacc gctcttcggt ccgtcagtag
     1321 cttggcctct ctggcccggg agaggatcag cagatcggtg accaggtcta agagcgccct
     1381 tgtgcgcgtc tccgccctgg agaccatctc ccgctgggtg gcggatacct ctccggtcag
     1441 tccttccagc acacccttca aagagctctg gatggccgtg aggggtgcct tcatctcttg
     1501 ggacatccgg cccatgtgca ggctcttaag ctgctccagc tctgccaggt ggttgtaggc
     1561 cacctccagc tctcgggctt tctgctgcag ctcctgggtc aactggagga tctcctggtc
     1621 ccgctccctg agccgcttca ctatggaggt ggccatgtac gcggacaggt agatcgtgct
     1681 gctgaacacc agcagggcta cggcgacgaa ctggccgtcc gtggccaggt cgggtcggac
     1741 cggcccgaac agggtgatgt gggggatcgc gccggcgtac tcgcccagca ccagcgcgcc
     1801 gaataggaaa atggctacag tggactggac gaaggtctcc cacctggaga gcaggatgct
     1861 ggcgatgatg acgtggaaga tgaagtagaa ggagagcggg ttctccacgc cgcccgtgaa
     1921 gtgaataagc aatgtgagga agaccagatc ggtcacgatc tgaccgtgcg ccatcccctt
     1981 ggcgtggtct aggcggaggg ccttgcttgc ctcctcggtc cacttgagac cgatgataaa
     2041 gagggcgttg tacatggcga tggcggcgcc gatgagataa aggggcagtg gggccaacct
     2101 tatccctgcc actgtagtgg ccaggaacgt gccgtagagg actgtcgtgg cagccagcca
     2161 gcggagctgg ataagccagt ggatccgctg ggttagctcc acctccacag gggccgattg
     2221 tcgcaacttc tcctccactc ttcggggccg gcatctactc ggccccttcc ctaaatgtaa
     2281 agcgcccctt ccacggcatg cgaccgcacg gtcaggtgcc gggaaggagg cgctcatcac
     2341 ctccggtatc aggcgcccgg tttggaacgc ctcccgaaag atgctaccaa atcaacccca
     2401 aaatggcaag atatgtgaag tagctaacta ttaaccatgc cggacttcac cctgctgcgc
     2461 agccactcca gccagccctc catcccccgg cccgtcctgg ccgacagctc gaagatcggc
     2521 agctctcggt tggtggcccg ggcggcggac ctggccctct cgaagtcgaa gtccaccatc
     2581 cccgtcagat ccagcttgtt cagcaccatc gcgctggact gctggaagat cagagggtac
     2641 ttttccggct tgtcgtcacc ctccgccacg ctcaggatca ccaccttgat ctcctcgccg
     2701 atgtcgaaac ccgcgggaca taccaggttg cccacgttct cgatgaacag caggtcgatc
     2761 tcctcgagcg gcagatcctc cagcgccgag cgcaccatgt ttgcgttcag gtggcagtcc
     2821 cctccggtgt tgatct
//
LOCUS       DATALS010000312         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_256969, whole genome shotgun sequence.
ACCESSION   DATALS010000312 DATALS010000000
VERSION     DATALS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256969"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VHS06_06915"
     CDS             complement(<1..445)
                     /locus_tag="VHS06_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987885.1"
                     /translation="MNTPRDAAKGSTSTIAAIVILAIIVGVGAALAAGYAQGVPPWNA
                     PDEPAHYNYIRHIATTGQLPELKHGNWDAPLLERLKSAKFPQSESVDSIAYESHQPPL
                     FYLMATPIYSLTASAPLLERVHALRRLSVVLATATLLLGFLAVRAI"
     gene            complement(722..>1690)
                     /locus_tag="VHS06_06920"
     CDS             complement(722..>1690)
                     /locus_tag="VHS06_06920"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2987886.1"
                     /translation="KEGLKDRPITRDIEWGVPIPLPGYENKRIYVWFDAVTGYLSASI
                     EWAKLRGEPDKWKEWWEEPCRAYYFVGKDNIPFHAIIWPAMLMGRGGLNLPYDVPANE
                     YLNLEGRQFSTSRNWAAWLPDMLGQFDPDPIRYALTINMPETRDTDFSMAEFVRRNND
                     ELVATYGNLVHRTLTFLQRYYEGKVPEVGPDYQGDERVIARVQETFDTVGDLLAGCHF
                     KDSLKEVMNLAQFGNRYLEETAPWKQRKVDPEAAGKSLYECLRMIDSLKVLTYPYLPF
                     SAEKLHGLLGYTDDLVSRGWKPTVPAPGTKLPEPAPLFTKLELPEA"
BASE COUNT          277 a          525 c          566 g          322 t
ORIGIN      
        1 agatcgcccg cacggcaagg aatcccagca gcagggtcgc tgtggcgagc accaccgaga
       61 gcctcctcag cgcgtggacg cgctccagca gcggtgcgct ggcggtgagg ctgtagatcg
      121 gcgtggccat caggtagaag aggggtggct ggtggctctc gtaggcgatg gagtccaccg
      181 attccgattg ggggaacttt gcactcttca gccgctccag cagcggcgca tcccagttac
      241 cgtgcttcag ctcgggcagt tgtccggtgg tggcgatgtg gcggatgtag ttgtagtgcg
      301 cgggctcgtc gggcgcgttc caggggggaa caccctgggc gtaacctgct gccagcgccg
      361 cccccacccc cacgatgatg gcgaggatca cgattgcggc gatggtggat gtggaccctt
      421 tggcggcgtc gcggggtgta ttcaaacgtg gcctcacggt aaatcgacgg ggaatggtag
      481 cctcaaccga agaggctgga accacgtatc gtgaatcgta gggcggggtc ttgtgccccg
      541 cctagagcgt ggtcagaatc accacggtgg tcgtgactgc ggcgtgggag gcggggcaca
      601 agaccccgcc ctacattctt ggacaatgcg ggcgacttgc cctcaccagt gtcgcccaat
      661 cacgctttgg gggcgacgtg agtcgcccca aacaacgcca cgcgccgcgc tccaagcgat
      721 cctacgcctc gggtagctcc agcttggtga agaggggtgc cggttccggc agcttcgtgc
      781 cgggtgcggg caccgtcggc ttccagcccc gcgataccag gtcgtccgtg tagcccagca
      841 ggccgtgcag cttctcggcg gagaagggca ggtatgggta ggtgagcacc ttcagcgagt
      901 ctatcatccg cagacactcg tacaggctct tgcccgccgc ctccgggtcc accttccgct
      961 gcttccaggg tgccgtctcc tcaaggtagc ggttgccgaa ttgggccagg ttcataacct
     1021 ctttcagcga gtccttgaag tggcagcccg ccagcagatc gcccacggtg tcgaaggtct
     1081 cctggacgcg cgcgatcacc ctctcgtcgc cctggtagtc cggccccacc tcggggacct
     1141 tcccctcgta gtagcgctgg aggaaggtga gggtccggtg gaccaggttg ccgtaggtgg
     1201 ccaccagttc gtcgttgttt cgccgcacga actccgccat tgagaagtcg gtgtcccgag
     1261 tctccggcat gttgatggtc agcgcgtagc ggatcgggtc cggatcgaac tgccccagca
     1321 tgtcgggcaa ccaggccgcc cagttgcggc tggtggagaa ctgccggccc tccaggttca
     1381 ggtactcgtt ggccggtacg tcgtagggta ggttgagtcc accccgaccc atcagcatcg
     1441 cgggccaaat gatggcgtgg aaggggatgt tgtccttacc cacgaagtag taggcgcggc
     1501 agggctcctc ccaccactcc ttccatttgt cgggttcgcc ccgcagcttg gcccattcga
     1561 tggacgcgga gaggtagcct gtaacggcgt cgaaccagac gtagatccgc ttgttctcgt
     1621 agccgggcag cgggatcggc acgccccact cgatgtcgcg ggtgatgggc cgatccttca
     1681 acccctcctt
//
LOCUS       DATALS010000313         7180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_167446, whole genome shotgun sequence.
ACCESSION   DATALS010000313 DATALS010000000
VERSION     DATALS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167446"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VHS06_06925"
     CDS             <1..351
                     /locus_tag="VHS06_06925"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEX2987887.1"
                     /translation="RTPEKVREYAKAAKERGIQLIIAGAGMAAHLPGVIAAWTTLPII
                     GVPLPAGELRGQDALYSMVQMPSGVPVATFGIGNSGARNAAYFAASVLSLSDESVRRS
                     YEDFRKRQSGEPVG"
     gene            456..1748
                     /gene="purB"
                     /locus_tag="VHS06_06930"
     CDS             456..1748
                     /gene="purB"
                     /locus_tag="VHS06_06930"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX2987888.1"
                     /translation="MIPKYSLPEMSRIWSEENKMDKWLAVEIASCEGWAELGEISQDD
                     IAKIRKAKYDLKRIDEIQAVSHHDVISFLRSVAESLGPESRFVHLGLTSSDVIDTALS
                     LQLLDAVDLIHRDLEKLEAALARQAVTYKDTVMIGRTHGIHAEPTTFGFKLAGWVAEA
                     RRNLKRLEQAREEVAVGKISGAVGTHANVPPDVEDFVCKKLGLQPVEVSTQIIQRDRH
                     ARLMVTLAVIASSLEKMATEIRALQRSDIGEAAEPFSEGQQGSSAMPHKRNPELTERV
                     TGLARLIRGYSIPAMEDVALWHERDISHSSVERVIFQDAFLALDYILNLFTGVMDRLD
                     VFSDRMRENLESTRGLVYSGRVLTALIEKGMKRQDAYEMVQRNAKKVWAEKVSLYDLL
                     AADPEVRKLLSEEELRELFDYRWHLRYIDVGFKRLGLI"
     gene            1745..2674
                     /locus_tag="VHS06_06935"
     CDS             1745..2674
                     /locus_tag="VHS06_06935"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX2987889.1"
                     /translation="MRVHSYLAGTGPAPEPVVRTDLPGVPLFARGKVREIYDLGDRLL
                     LVASDRISAFDVVMNEPIPGKGIVLTSLSAFWFEATADLLANHYISVDPADFPAELRS
                     YSEMLSGRAMLVKKAERIDIECVVRGYLAGSAWSEYEKDGTVAGVALPVGLRESDKLE
                     KPIFTPATKAESGHDINISVDEMKKLAGVDLTETLVRKSIDLYNFAEQRARSRGIILA
                     DTKFEFGLIDGKATLIDEVFTPDSSRFWPADQYEPGRSQPSFDKQYLRDYLLSVGWNK
                     EPPPPTLPAEVVAKTGEKYRDAYVRIVGRIPED"
     gene            2792..3040
                     /gene="purS"
                     /locus_tag="VHS06_06940"
     CDS             2792..3040
                     /gene="purS"
                     /locus_tag="VHS06_06940"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX2987890.1"
                     /translation="MWLAKVHVTLKPVVLDPQGQAVQGGLQQLGFDTVISVRAGKYLE
                     IKLEAPDKADAERLVNEMCDKLLANPVIEQYRFEVAES"
     gene            3332..3793
                     /locus_tag="VHS06_06945"
     CDS             3332..3793
                     /locus_tag="VHS06_06945"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX2987891.1"
                     /translation="MAGSGRERGQGIVEFALVIPIVVALILGVVELGFLLYDYESLVA
                     GTRAAARAGAVYKIVEGCPNNDQNRFYGTGCTPAYGDNMVATLQRNTSVRLGNPPTIV
                     DPPVWTLAVPPIDTRQGDLMTITVNYQHRFLTGFFGQATIPLSASASARIE"
     gene            3832..4335
                     /locus_tag="VHS06_06950"
     CDS             3832..4335
                     /locus_tag="VHS06_06950"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX2987892.1"
                     /translation="MARREKGQSLVELALTLPVVLLLLTGVVDLGFVLYAHIQVASAT
                     GEASRVAAHFVGDATANCGNNDTARLTLVKKAVYDSTVSPATSSLGRLSPTSPYFGIG
                     SYTAANAGDDVWIDGYAACGAGTETRSGQEMVVHVRYREPVLFGAIPGLSSMRFTLSS
                     AARVRIP"
     gene            4336..6255
                     /locus_tag="VHS06_06955"
     CDS             4336..6255
                     /locus_tag="VHS06_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX2987893.1"
                     /translation="MKTLNSRSESGQVLIIVAAAIFFVMLGAAAVAVDIGNGFLNKQR
                     LQNTADAAALAAAYRVASGGSDAAAVRLAAVTAAQSIVTQNTGGTVTLTDRGTGSDPN
                     LADGIQISGNDVRVVLNKNDVKTFFAGAMGVGNISVIARSRAKVNAGGGVMPIAVKRY
                     SAGNTLYQLGTNGNPGSVTDYLAQATNGPSGSWPGSWPLDLLVVDASPAASLAAPGPV
                     IPILGRDAQANEAQGNDFHFWVAPDVRNITWSYPPGPTYTNGITAGSSAQDLKNLESS
                     YFSPIRPGYNSVEGLPHQRDQVAVLNGVDTQNTVGEMEKYYGPGKYVIAMIYDGSVHR
                     KPEFQLQVNPTLRQDNHGTLLPLDYTVTLHQTNATYPYWGNGIGLSVVGIESDFATWN
                     FSQNPVDVAGADKSVTLTITAAHRTDPTPPATEPLKTSGARSFLIRAYDNLNHVQRTA
                     TAAVMFGNTAAYMATCSSAYQQIQQGSSGQFIVDLTGYNGYTNHTISVGSPQWEGPAP
                     SSGTVHVGYVNHNPDSVQTTSVNNVRDGHTSTFNVYLDVDPGASTGQYILRIPMSDGS
                     VTQNMYLSVEIYPTTSGTTVGNMVSFVYILGYAAFEIDHYTNNTVYAKAVSGLVADPS
                     SLMVGSAVRLLGWNQ"
     gene            complement(6280..>7180)
                     /locus_tag="VHS06_06960"
     CDS             complement(6280..>7180)
                     /locus_tag="VHS06_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2987894.1"
                     /translation="SIPAAVVMSKADSLPTAITAALVLGFALGMYLPLGMGLIVDMGR
                     GGRGPAYMSVHELAATLASFIGSAAVALLLAWTDWHGSILAWCVVGVLATVAFVAVRD
                     PEGDRRHVEKVREHVPVDFRLFCAVFSYGIGTMLVMGLVSMLALIMVRAQGLDQVHAA
                     SVLGYTRLAGLLGVVAVALLADRLGHVRVLMGLQMVALAGLVLMSLDGFGLVFVAGVV
                     LLAVGASGNITLNPVVIAEAFPSGQRERVMAYATGPGALLGTVVAPALFGVMLDAGLS
                     TGPVLFAAGATLVAIFLTGRIAR"
BASE COUNT         1528 a         2199 c         2201 g         1252 t
ORIGIN      
        1 cgcacgccgg agaaggtgcg cgagtacgcc aaggccgcca aggaacgggg catccagttg
       61 ataatcgccg gagcaggcat ggcagcccat ctacccggag tcatcgcggc ctggacaact
      121 ctacccatca tcggcgttcc ccttcccgcg ggtgagctac gggggcagga tgccctctac
      181 tccatggtgc agatgccctc aggggtgccg gtggccactt tcggcatagg taacagcggc
      241 gctaggaacg cggcctactt cgcggcatcg gtgctgagcc tgagcgacga gtccgtccgg
      301 cgcagctacg aggatttccg caagaggcag agcggcgagc cagtaggata gacgaagtga
      361 tgagtaatga gtaatgagtg atagacacag acagctcatg actcatcact cataactcat
      421 cactcattac ttggagttgg cgaggaggca gacccttgat accgaagtac tctcttccgg
      481 agatgagccg catctggtcg gaagagaaca agatggacaa atggctcgcc gtcgagatcg
      541 cgtcatgcga gggctgggca gagctcggcg aaatctcaca ggacgacatc gcgaagatcc
      601 gcaaggcgaa gtacgacctc aagcggatcg acgagatcca ggccgtcagc caccacgacg
      661 tcatctcctt cctccgctcc gtggcggaga gcctggggcc ggaatcgcgt ttcgtccacc
      721 tgggactcac ctcctccgac gtcatcgaca cggcgctctc cctccaactt ctggatgcgg
      781 tggacctgat ccaccgcgac ctcgagaagc tggaagccgc cctcgcccgg caggccgtga
      841 cgtacaagga cacggtgatg atcggccgga cgcacgggat ccacgcggag ccgacgacgt
      901 tcggcttcaa gctcgcgggg tgggtcgcgg aggcgaggcg caacctgaag cgcctggagc
      961 aggcgaggga ggaggtcgcg gtcgggaaga tatctggagc ggtgggaacc cacgcaaacg
     1021 tgccgccgga tgtggaggac ttcgtctgca aaaaactggg gctccagccg gtggaggtct
     1081 ccacgcaaat catccagcgc gaccgccacg cccggcttat ggtgacactc gccgtgatcg
     1141 cttcctcgct ggagaagatg gcgacggaga tcagggcgct ccagcgcagc gacatcgggg
     1201 aggccgccga gccgttttcc gagggacagc agggctcctc cgcgatgccc cacaagcgca
     1261 accctgagct gaccgagcgg gtcaccggtc tcgcgcgcct tatccgcggg tactccatcc
     1321 cggcgatgga ggatgtggcg ctatggcacg agcgcgacat cagccactcc tcggtggagc
     1381 gcgtgatctt ccaggacgcc ttccttgcgc tggactacat cctcaacctg ttcaccgggg
     1441 ttatggaccg gctggacgtc ttctccgacc ggatgagaga gaacctggag tccacccgcg
     1501 gcctcgtgta ctccggccgc gtcctcacgg ctctgataga gaaagggatg aagcgccagg
     1561 acgcctacga gatggtccag cggaacgcga agaaggtctg ggcagagaag gtgagcctgt
     1621 acgacttgct ggcggcggat cctgaggtcc gcaagctcct ctccgaggaa gagcttcggg
     1681 agctgttcga ctaccgctgg cacctgcgat acattgacgt gggcttcaag aggttaggac
     1741 tgatatgaga gtgcattcgt acctggccgg gaccggcccc gcccccgaac ccgtcgtccg
     1801 caccgatctt ccaggcgtcc cgctcttcgc gcgcggcaaa gtgcgagaga tctacgacct
     1861 gggagacagg ttgctactgg tcgcctccga ccgcatctcc gccttcgacg tcgtgatgaa
     1921 cgagccgatc cccggaaagg gcatcgtgct gacctcgctc tccgccttct ggttcgaggc
     1981 gacggcagac cttctggcga atcactacat ctccgtggac ccggcggact tccccgcgga
     2041 gctccggtca tactccgaga tgctctccgg ccgggcgatg ctggtgaaga aggccgagcg
     2101 aatcgacatc gagtgcgtcg ttcgcggcta tctcgcaggg tcagcgtggt cggaatacga
     2161 gaaggatggc acggtcgcgg gagtggcgct gccggtggga ctgcgagagt cggacaagct
     2221 agagaagccg atctttaccc ccgcgaccaa ggccgagtcc ggacatgaca ttaacatttc
     2281 tgtcgacgag atgaagaagc tggcaggcgt ggatctcacc gagaccctgg tgaggaagag
     2341 catcgacctc tacaacttcg ccgagcaacg cgcccgctcg cgcggcatca ttctggccga
     2401 cacgaagttc gagttcgggc tgatcgacgg caaggcgacg ctgatcgatg aggtgttcac
     2461 gccggactcc tcgcgcttct ggccggcgga tcagtacgag cccggcaggt cgcagccaag
     2521 cttcgacaag caatacctcc gcgactacct gctcagcgta ggctggaaca aggagccgcc
     2581 cccgcccacg ctgccagccg aggtggtcgc gaagaccgga gaaaagtacc gcgacgccta
     2641 cgtccgcatc gtcggtagaa ttcctgagga ctgaggactg aggactgagg actgaggact
     2701 gagaggagtg tagcgtggag cgcggccgtc ccgcccgtcc ccggtccccg acggtaggat
     2761 tcctcccacg aggagatgag gtgagactag aatgtggcta gctaaggtac acgtgacact
     2821 caagccggtg gtactggatc cacagggtca ggccgtgcaa ggtggactcc agcagctcgg
     2881 cttcgatacg gtgatcagcg tccgcgccgg aaagtacctg gagataaagc tggaggcgcc
     2941 ggataaagcc gatgctgagc gccttgtgaa cgagatgtgc gacaagctcc tcgccaatcc
     3001 cgtcatcgag cagtaccggt tcgaagttgc cgaaagctga ggccgcgagt caagaacccg
     3061 cagctgtcag aaacctgcca ttatcatgac aattctttca tgatcgtgca ggtggggtgc
     3121 cccgcgctca gaagatctga tttgaatgac aatcctgtaa tcctcgccag tatcttcata
     3181 cacctctttc ttatcctctc gaattaggct acattacaga tccgttcttg acagtcgctg
     3241 gagtctccgt ttaatcggta ctgcattcac gataatggtg ttgatgatgt catctagtcc
     3301 ttgccgcgag cagaagaggc gcggcgcact cttggccggg tccgggcgcg agcgagggca
     3361 gggcatcgta gagttcgccc tggtaattcc aatagtggtt gcactcattt tgggagtggt
     3421 ggagttgggt ttcctcctct acgactatga gagcctagtc gcgggcacga gggcggcagc
     3481 gcgggcgggg gcagtctaca agatcgtcga ggggtgtccg aacaacgacc agaatcggtt
     3541 ctatggtaca ggttgcacac cggcgtacgg ggataacatg gttgccacgc tgcaaagaaa
     3601 tacgagcgta cgtctcggta atccccccac aatagttgac ccgcccgttt ggactctagc
     3661 cgttccgccc atagacactc gtcagggcga cctgatgacg attactgtga actaccagca
     3721 tcggttcctg acggggtttt tcgggcaggc gacgatcccg ctctctgcca gcgcttcggc
     3781 aaggatcgag taataatggg aatttgcatc atccccgaac gcattgcgaa gatggcccgc
     3841 cgagagaaag gccagtccct agtggaatta gcgctgactc tcccagtagt cttgcttctg
     3901 ttgacgggag tggtcgactt gggctttgtc ctctatgccc acatccaggt ggcctcagcc
     3961 acgggcgagg catcccgggt cgcagctcac ttcgtgggcg acgccacggc gaactgcggg
     4021 aacaacgaca ccgccaggtt gaccctggtc aagaaggcag tttacgactc gaccgtgagc
     4081 ccggcgacca gctcgcttgg acgcctctcg ccgacatccc cctacttcgg catcggcagc
     4141 tacacagcag ccaatgctgg agacgatgtg tggatcgatg gttacgccgc atgcggtgcg
     4201 ggcaccgaga caaggagcgg ccaggagatg gtcgtacacg ttagatacag ggagccggtg
     4261 ctgtttgggg caatcccagg gctgagcagc atgcgattca cgctgagcag tgccgcgaga
     4321 gtgaggatcc cgtagatgaa gacgctgaac agcagatcgg aaagcggcca ggtcctgata
     4381 atcgtcgccg cggcgatctt ctttgtcatg ctcggcgcgg cggccgttgc cgtggacatt
     4441 ggcaatggct tcctgaacaa gcagaggctt cagaacactg cggacgccgc ggcgttggcc
     4501 gccgcgtacc gggtagccag cggtggctca gacgcggcgg cggtacgcct ggctgcagtg
     4561 acggccgccc agagcatcgt gacacagaac actggcggta ccgtgaccct gacagaccgc
     4621 ggcaccggct ccgatccgaa cctcgctgac ggtatacaga tctccggtaa cgacgttcgt
     4681 gtagtgctca ataagaacga tgtgaagacg ttttttgccg gcgcgatggg cgttggcaat
     4741 atctcggtga tcgccaggtc tagagccaag gtcaacgcgg gcggtggcgt gatgcccatc
     4801 gcggtgaagc gatactcggc cgggaacacg ctttaccaac ttggaaccaa cggaaacccg
     4861 ggctccgtaa ccgactatct ggcgcaggcc acaaatggtc caagcggaag ttggccaggg
     4921 tcatggcccc tggatcttct cgttgtcgac gcaagccccg cggctagcct ggcggcacca
     4981 ggtccggtta tcccgattct gggtagggat gcacaagcga acgaagctca gggcaacgac
     5041 ttccactttt gggtagcgcc ggacgtacgg aatatcacct ggtcataccc gcccggaccc
     5101 acgtacacca acggcataac cgcgggcagc tcagctcaag acctcaagaa tctggagagc
     5161 tcgtatttct ctcccataag accaggatac aactccgtcg aaggccttcc ccaccaacgc
     5221 gaccaggttg cggtgcttaa tggcgtcgac acacagaaca ctgttggaga gatggaaaag
     5281 tactacgggc cgggcaagta cgtcatcgcc atgatctacg acggttcggt gcaccgaaag
     5341 cctgagttcc agttgcaggt gaacccaacc ttgaggcagg acaaccatgg caccctactg
     5401 ccgctcgatt acacagtgac tctgcaccag accaacgcga cctaccctta ctggggtaac
     5461 ggcatcggcc tctctgtagt agggatcgag tctgacttcg cgacgtggaa cttctcccaa
     5521 aatccagtgg acgtggccgg cgcggacaag agcgtgacat tgaccatcac agcagctcac
     5581 cgcactgacc cgacgccacc cgcgacggag cccctgaaga cctcgggggc caggtccttc
     5641 ctcatccggg catacgacaa cctgaaccac gtccagcgca cggccactgc agccgttatg
     5701 ttcgggaaca cagcggccta tatggcaacg tgcagttcgg cgtaccagca gatccagcag
     5761 gggtcgtcgg ggcagttcat agtagacctg acgggttata acggctacac caaccacacg
     5821 atttcggttg gttccccgca atgggagggg cccgcccctt cctcaggcac agtccatgtc
     5881 ggctatgtta accataatcc agacagcgtt cagaccacct cagtaaacaa cgtgagggac
     5941 ggccatacaa gcaccttcaa tgtctatctt gatgtcgatc ctggggcatc gaccggacag
     6001 tacatcctga ggataccgat gagcgacggc tcggtgaccc agaacatgta cctgtcggtc
     6061 gagatctacc ccacgacgag cggaacgacc gtcgggaata tggtgtcctt cgtctacatt
     6121 ttgggctacg ccgccttcga gatcgaccac tacaccaaca acaccgtcta cgccaaggca
     6181 gttagcggcc tggtcgccga tccgtcctct ctaatggtgg gttcggcagt acgactttta
     6241 ggttggaacc agtaggggac gttcgcggga accaggaggc tatctcgcta tccgtcccgt
     6301 gaggaagatc gccaccaggg tcgcccccgc ggcgaagagg acggggcccg tggaaagccc
     6361 cgcgtcgagc atgaccccga agagggcggg cgccacgacc gtcccgagca acgcccccgg
     6421 cccggtcgcg taggccatca ccctttcgcg ctggcccgat gggaatgcct cggcgatcac
     6481 caccggattc aaggtgatgt tgccagacgc gccgaccgcg agtagcacca ccccagcgac
     6541 gaacaccagg ccgaagccgt ccaacgacat cagtacgagg ccagctaacg cgaccatctg
     6601 aagtcccatg agcacacgaa cgtgccccag gcggtccgcc agaagagcga ctgccaccac
     6661 tccaagcagc cccgccagtc tggtgtaccc gaggaccgac gcggcatgca cctggtccag
     6721 cccctgggcg cggaccatga tcagcgccag catagacaca agccccatca ccaacatcgt
     6781 gccgatgccg tagctgaaga cagcgcagaa cagcctgaag tcgaccggca cgtgctctcg
     6841 aaccttctcc acgtggcgtc gatcgccctc gggatcccgc acggcaacga atgccaccgt
     6901 ggccaggact ccaaccacgc accaggccag tatcgacccg tgccagtcgg tccaggccag
     6961 caagagcgcc actgcggccg aaccgataaa cgaggcaagt gtggccgcga gttcatggac
     7021 ggacatgtag gcgggaccgc gtccgccgcg tcccatgtct acgatcaagc ccatgcccag
     7081 gggcaggtac atccccaggg caaatcccag gaccagggca gccgttatgg cggttgggag
     7141 actgtccgcc ttcgacatca ctacggcagc cggtatggag
//
LOCUS       DATALS010000314         5205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_60565,
            whole genome shotgun sequence.
ACCESSION   DATALS010000314 DATALS010000000
VERSION     DATALS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60565"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VHS06_06965"
     CDS             complement(<1..566)
                     /locus_tag="VHS06_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2987895.1"
                     /translation="MAKKVLVTEFIHPAGIEMLKKECEVVTPTGISVPELKAAIADVD
                     GVLVRVAPMNREVMEAGKKLKVIGKHGVGTDNIDIPVATELGIAVCNTPQANGEGVAS
                     LALTLMLSLSRHVTQCDAFVRGGGWKDKDSLMGTELLGKTLSIVGTGRIGGRLAQMCQ
                     SAFDMKVYAFDPYVSKEAMAEKGIIKVET"
     gene            complement(698..1387)
                     /locus_tag="VHS06_06970"
     CDS             complement(698..1387)
                     /locus_tag="VHS06_06970"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX2987896.1"
                     /translation="MKINVGSSVKTSEGEEIGHVERVILDPDTKDVDAIVVHRGLILT
                     RDVIVPLSLVQYADQSGVHLRMTKDQLHELPDFVERHYVARPGESSVAYPYTPGSILF
                     PLAPTYGAPGLPANYQPLGQQEVREPSEDVEIAEGTEVRAVDGTIGRVGEVRTDPEAD
                     RVTEFTVRRGFGLTRDAVIPIEYVDQVADDHIKLSLTIDQVEHLPIPITDRYITREKP
                     KGAKPRRRKKG"
     gene            1658..2059
                     /locus_tag="VHS06_06975"
     CDS             1658..2059
                     /locus_tag="VHS06_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987897.1"
                     /translation="MWNPTPTIGDLGIDPAAVRKLLPHLDSFVKWDLLRFLHEAPDIP
                     TRVEDLARYLGRDETELKPAASALAVAGILKQEEKGGSYRYSLVEDEEIRELLGHLVE
                     RYQEDRLVRLAISAGILKAQRNANRLIYAAR"
     gene            complement(2120..2764)
                     /locus_tag="VHS06_06980"
     CDS             complement(2120..2764)
                     /locus_tag="VHS06_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEX2987898.1"
                     /translation="MRRITSIEPQSRPGSRRINLYLDGQYAFSLDEEVAAALSVDTMI
                     SDAEIADLQRRDRLHQVYDAALTLLTYRPRSASELRSRLLRRSFDPELVDEALQRLKT
                     QKVVDDELFAQFWVENRMSYSPRGGRLLKAELRAKGIEREIIEGALPEAEEEEQAAYK
                     VAERKARSLSGLEWQEFRHKLGDHLVRRGFDYDTTSSIVRRIWEEIHQQVPDEE"
     gene            complement(2827..3882)
                     /gene="recA"
                     /locus_tag="VHS06_06985"
     CDS             complement(2827..3882)
                     /gene="recA"
                     /locus_tag="VHS06_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX2987899.1"
                     /translation="MVLDKEKEKALDLAIGQIERSFGKGSIMKLGEDSSRLGVEVIPT
                     GSIALDIALGVGGIPRGRVVEIYGPESSGKTTVVQHIMAEAQKMGGVAALIDAEHALD
                     PGYAQHCGVNIDDLLVSQPDTGEQGLEIAEALVRSGAVDVVAIDSVAALVPRAELEGD
                     MGDSHVGLQARLMSQALRKLAGAINRSKTCVIFTNQLREKVGVMFGNPETTPGGRALK
                     FYASIRLDIRRIESIKQGTEVMGNRTRVKVVKNKVAPPFRQAEFDIMYSQGISKEGGL
                     LDVGTELGIVKKSGAFFSFGDSRIGQGRENSKEFLRKNPEMAGEIERQIRQTVSAGKP
                     LPLTAAVTVGAESEDAE"
     gene            complement(3961..4455)
                     /locus_tag="VHS06_06990"
     CDS             complement(3961..4455)
                     /locus_tag="VHS06_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009181575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX2987900.1"
                     /translation="MSGQAEEELIASRSAALGPGLRARGWTLATAESCTGGGLSDAIT
                     NVAGSSDYFLGGVVSYSNAVKEALLGVDSGVLATQGAVSEPVARQMAEGVRRVIGADL
                     GIGITGVAGPGGGSPGKPVGLVFIAVSGPAGTEVRRFQWGGDRIANKRHSVAAALEML
                     LALL"
     gene            complement(4458..>5205)
                     /locus_tag="VHS06_06995"
     CDS             complement(4458..>5205)
                     /locus_tag="VHS06_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2987901.1"
                     /translation="LLYSIAERVPDLPWIRVLYLYPQRITPTLMRAMAELPQICNYVD
                     IPLQHTHPDLLKRMRRPHDEVNRVVDSIREAIPEVAIRTTFIVGFPGETEEEHRHLLA
                     TIDALRFDHVGVFTYSPQAGTPAAAMDGQIPETTKERRWRETMELAQRISLEQNRRLV
                     GQEMDVLVEGVGRGGRRHQSTMAGRSYRDAPEVDGLVFFNGKAHVGDLVRVRITSALE
                     YDLVGEPTNGDEVLGKRQRGGKPHRKERRG"
BASE COUNT          971 a         1751 c         1463 g         1020 t
ORIGIN      
        1 gtctcgacct tgatgatccc cttctccgcc atggcctctt tgctgacgta cggatcgaag
       61 gcatagacct tcatgtcgaa agccgactgg cacatctgag caaggcggcc gccgatacgg
      121 ccagtcccga cgatggagag ggtcttgccc agaagctcgg tgcccatcag cgagtccttg
      181 tccttccagc ctccgcctcg gacaaacgca tcgcactgag tgacgtgcct ggacagcgac
      241 agcatcagcg tcagagcgag gctggcgaca ccctcgccgt tagcctgcgg ggtgttgcag
      301 accgcgatcc ccaactcggt ggcaaccggg atatcgatgt tgtcggtacc gactccgtgc
      361 ttgccgatca ccttcaactt cttgccggct tccatgacct cgcggttcat gggcgcaacc
      421 cggaccagaa caccgtcgac atccgcgatg gctgccttca actcggggac ggaaatgccg
      481 gtgggagtaa ccacctcaca ttccttcttc aacatctcga tgccagccgg gtggatgaac
      541 tcagtaacca gcaccttctt cgccatacta tgcactcctt cgtggctatc gatcttaggg
      601 gataaccggc gtgcataata gcagcaactg acatctcagg ccaacagtgc ggacgggggg
      661 ggaactcggc gaaccccgag ttcccccgaa ttccccctca ccccttcttc cgtctccggg
      721 gcttcgcacc cttaggcttc tctcgggtga tgtacctatc ggtaatcggt atggggagat
      781 gctccacctg gtcgatggtg agagagagct tgatgtgatc gtccgccacc tgatctacat
      841 actcgatagg aatcacggca tccctcgtca acccgaagcc tcgcctgacg gtaaattctg
      901 tgacccggtc cgcctctgga tcggtgcgca cctctccaac ccttccgatg gtgccgtcaa
      961 cagcacgcac ctctgtgccc tccgcaatct cgacatcctc gctgggttcg cgcacctcct
     1021 gctgccccag gggctggtag ttcgccggca agccgggcgc tccataggtc ggcgccaggg
     1081 ggaacaggat ggagcccgga gtatagggat aggccacact gctctccccc ggacgcgcca
     1141 cgtagtgccg ttccacgaag tccggcaact cgtgcagctg atcctttgtc atccgcagat
     1201 ggacccccga ctgatcggcg tactggacga gagagagggg cacaatcacg tcccgcgtga
     1261 ggatgagtcc cctgtgcacc acgattgcat ccacgtcctt ggtgtccggg tccaggatca
     1321 cccgttccac atgcccaatc tcttcgccct cgctcgtctt cacgctggaa ccgacattga
     1381 tcttcatcgc cgcctccctc tggaaaggtt ggagactggg ggcggcaata tgcgtaccaa
     1441 acaggggatg gcggttgggg atgggggaca ggagggcacc aagatccagc ccccaacccc
     1501 tatcccccaa ccctcgcgca gagaaatggc atagaacctg cacccctgct cttcggcaga
     1561 gtgagacgag gtttcgctga agcctgtcga acaaggatag tctcgggcag tgtcccaggg
     1621 ttcactgcga ggcacagccc accgaaaggg ggatccgatg tggaacccca ctccaaccat
     1681 aggtgatctg gggattgacc cagcggcggt gcggaagctt ctcccccacc tggactcctt
     1741 cgtgaagtgg gacctcctgc gcttccttca cgaagcaccg gacattccta ccagggtcga
     1801 ggacttagcc cgctatctgg gaagggatga aaccgaattg aagccggccg caagcgcact
     1861 ggctgtggcg ggaatactca aacaagagga gaagggggga agctatcgct attctctggt
     1921 cgaagacgag gagatcaggg agttgctcgg tcacctggta gagaggtacc aggaggatcg
     1981 gctggtcagg ctggccatct ctgcagggat cctgaaagcg cagagaaatg ccaatcgact
     2041 catctacgcc gcccgctgac ccagcggacc tgcagccgga ctccgcggct acagagcccc
     2101 aacggcattc gtgctaccgt cactcctcgt ccggcacctg ctgatggatc tcctcccaga
     2161 tgcgacgcac tatcgaagag gtagtatcgt agtcgaaacc acgtcggacc agatgatcgc
     2221 ccagtttgtg ccggaactcc tgccactcca gccctgagag cgagcgcgcc ttcctctcgg
     2281 ccaccttgta ggccgcttgc tcctcttcct ctgcctctgg aagggcaccc tcgatgatct
     2341 ccctctcgat ccccttggcc ctcaactcgg ccttcaggag cctgcctcct ctcggactgt
     2401 aactcattcg gttctcgacc cagaattggg caaacagctc gtcgtcgaca accttctgcg
     2461 tcttcagcct ctgcaatgcc tcgtccacga gctcggggtc gaaggagcgc cgaagcagcc
     2521 ggcttcgcaa ttccgatgcg ctgcgaggcc gataggtgag gagggttagg gccgcgtcgt
     2581 acacctgatg caggcgatcc cgacgctgca agtcggcaat ctcggcatca gagatcatgg
     2641 tgtctacgga gagggccgcg gcgacttctt cgtccagcga gaaggcatac tgcccgtcca
     2701 ggtacaggtt gatccggcgg ctgccgggcc tggattgggg ttcgatggag gttatgcgtc
     2761 gcatgcggat gttcccaggg gtaggggcga cgtatgcgtc gcccctactt tcgacgttga
     2821 ggttggctac tcggcgtctt cgctctcggc gccgacggtc acggccgcgg tcaagggcag
     2881 cggcttgccg gcagacacgg tctggcggat ctgccgttcg atctcccccg ccatctctgg
     2941 gttcttccgc aggaactcct tggagttctc gcgaccctgg ccgatcctgg agtcaccgaa
     3001 ggagaagaag gctccactct tcttgacgat ccccaactcg gtgccaacgt ccaacaggcc
     3061 gccctccttg gagatcccct ggctatacat tatgtcgaac tcagcctgtc ggaaaggtgg
     3121 cgcaaccttg ttcttgacca ccttcactcg agtcctgttc cccatcacct ctgtaccctg
     3181 cttgatcgac tcgatccggc ggatatccag tctgatggag gcgtagaact taagcgcgcg
     3241 gccgccgggc gtcgtttccg gattcccgaa cattacgccc accttctcgc gaagctggtt
     3301 ggtgaagata acgcaggtct tggagcggtt gatcgctccc gccaacttgc gcagcgcctg
     3361 ggacatcagg cgagcctgca ggccgacgtg ggagtcgccc atgtcgccct ccaactccgc
     3421 cctggggacc agcgccgcta ccgagtcgat tgccaccaca tccaccgcgc cgctgcgcac
     3481 cagcgcctca gcgatctcca acccctgctc gcccgtgtcc ggctgagaga ccagcaggtc
     3541 gtcgatgttg acgccacaat gctgcgcata gcccgggtcc agggcatgct cggcgtcgat
     3601 cagggccgcc acgccgccca tcttctgggc ctcggccatg atgtgctgga ctacggtggt
     3661 ctttcccgag gactcaggac cgtaaatctc gacgaccctg ccccttggga ttccaccgac
     3721 gcccagggcg atgtccagag cgattgagcc ggtagggatg acctcaacgc cgaggcggga
     3781 agagtcctcg cccagcttca tgatcgaccc cttgccaaag gagcgctcga tctgtccaat
     3841 agctaaatcc agcgccttct ccttttcttt gtcgagcacc atctgcccct cccgcgttca
     3901 tactgcgaca acactaacgc acaattcggt ggcactatta tagcacaaat gttcgaggac
     3961 tcagagaagt gccagcagca tctccaacgc cgcggccacc gagtggcgct tgttggcgat
     4021 gcgatcgccc ccccattgaa agcgacgcac ctccgtgccg gcaggtccgg atacggcaat
     4081 gaacaccagg cccaccggtt tccccgggct cccgcctcct ggtcctgcca caccggtgat
     4141 gccgatgccc aagtcggccc caatcacgcg cctcacccct tcagccatct gccgagccac
     4201 cggctcgctg accgcgccct gggtcgccag caccccggag tccaccccca gcagcgcttc
     4261 cttcaccgca ttcgaatagg acaccacacc gccgaggaag tagtccgaac ttccggccac
     4321 gttcgtaatg gcatcggaca atccgcctcc ggtacaggat tcggcggtgg ccagcgtcca
     4381 tccccgggcc ctcaggcccg gtcccaatgc agccgatcga gaggcaatca actcctcttc
     4441 cgcctggcca gacatcctca acctctcctc tccttgcgat gtggcttgcc gccccgctgt
     4501 ctcttgccca acacctcatc accgttcgtg ggctctccca caaggtcgta ctccagtgcg
     4561 gaggtgatgc gtaccctcac gaggtcgccc acatgcgcct ttccattgaa gaagaccagc
     4621 ccatctacct cgggggcgtc ccgatagctt cggcccgcca tcgtcgattg gtgacggcgc
     4681 ccgccgcgac cgactccctc cacaagcaca tccatctcct gtcccaccag gcgacggttc
     4741 tgctccagcg agatccgctg agccaactcc atcgtctctc tccaccgtcg ctccttggtc
     4801 gtctcgggta tctgcccatc catggctgcc gccggcgtcc cagcctgggg cgaataggtg
     4861 aagactccca cgtggtcgaa ccgcagcgca tcgatggtcg ccagaagatg tcggtgttcc
     4921 tcctccgtct ctcccgggaa gccgacgata aaggtcgtcc ggatagccac ctcagggatc
     4981 gcctcgcgga tggagtccac aactctattc acctcatcgt gagggcgtct catccgcttc
     5041 aggaggtcgg ggtgggtgtg ttgcagcgga atgtctacgt agttgcagat ctgtggcaac
     5101 tccgccatgg cacgcatcag cgtcggcgtg atccgctgcg ggtagaggta cagaacccga
     5161 atccagggca ggtcgggtac tctctcggcg atgctgtaga gaagg
//
LOCUS       DATALS010000315         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_330713, whole genome shotgun sequence.
ACCESSION   DATALS010000315 DATALS010000000
VERSION     DATALS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330713"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VHS06_07000"
     CDS             complement(<1..155)
                     /locus_tag="VHS06_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2987902.1"
                     /translation="MYKGSVLMTMTAVATRVETDAILTDRQIEILSLLLEGKSSKEVA
                     RMLYVSKR"
     gene            411..1526
                     /locus_tag="VHS06_07005"
     CDS             411..1526
                     /locus_tag="VHS06_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX2987903.1"
                     /translation="MTRESLSASPLEMVEKEQRVRHFLADQKLDGLVLTTQSNFAWFT
                     CGGDSHVGIGTDNGSVSILVTPDAKYVLCDNVEAPRFLNEELAGQGFEFRVFNWWEGG
                     PSDEIAKLVSGPVGADTPIPGTTLVAGKIPPLRYSLTAPEIERYRWLGKATGECLSEA
                     AHEIRPGMTEHEIAAVLGGKIMSRGIIPQLVLIAVDERISKYRHPIPTDKTLDRYAML
                     VAGAKKWGLVVSATRIVHFGAVPCELKQKHDAVMAVDAEFIANTRPGTSVDYIFRKGM
                     AAYERAGYPEEWHLHHQGGPTGYAGRDYRVKPGIQESVQPNQAFAWNPTITGTKCEDT
                     IIVLNDRTEIISAYGDWPMVPIEAESMTIVREDILEL"
BASE COUNT          339 a          484 c          494 g          318 t
ORIGIN      
        1 cgtttgctga cgtacagcat tctcgcgact tctttgctgg atttgccctc cagcaagaga
       61 ctcagaatct cgatctgacg gtccgtgagg atagcgtcag tctcgacgcg cgttgccact
      121 gcggtcatcg tcatgagtac actcccctta tacatcggga gcgtgcagac taccctgcag
      181 acgcccctat cccttgctct gcctccattt tacccacaac tgatgagttc taaccctact
      241 gctgcgtctt ctcctctgcg catggcttgg gtatgggact ccgtgggtcg ccgcctctat
      301 tagtgcaagt tgcgtgccaa aagtgccgta ttgagcgact gtgcaggaaa ggtttgtggc
      361 acgaacgaac aagtccttgt acgctcaaaa catggatcgg aggcttcacg gtgaccaggg
      421 aatctctctc ggcaagtcca ctggagatgg tggaaaagga gcagcgggta aggcactttc
      481 tcgctgacca gaagctggac ggacttgtcc tcaccaccca gtccaacttc gcctggttca
      541 cctgcggagg cgacagccac gttgggatcg gcacggacaa cggcagcgtg tccatcctcg
      601 tgactcccga cgcgaagtac gtcctctgcg acaacgtgga ggccccgcgc ttcctcaatg
      661 aggaactggc ggggcagggg ttcgagttcc gtgtgttcaa ctggtgggag ggcggcccgt
      721 ccgacgagat cgccaaactc gtgagcggac cagttggcgc cgacaccccg atcccaggga
      781 cgaccctcgt cgcgggcaag atccctccgc ttcggtattc gctgaccgca cccgagatcg
      841 agcgctaccg atggctgggc aaggccaccg gcgagtgcct gtcagaggcg gctcacgaga
      901 tccgtccggg catgacagag catgagatcg cggccgttct tggcggcaag atcatgagcc
      961 ggggcatcat cccacaactg gtgctgattg ctgtggacga gcgtatatcc aagtacaggc
     1021 acccgattcc caccgacaaa accttggacc ggtacgccat gctggtggcc ggcgcgaaaa
     1081 agtgggggct ggtcgtctcc gcgacgcgca tcgtgcactt cggcgcggtg ccgtgtgaac
     1141 tcaagcagaa gcacgacgcg gtcatggcgg tggacgcgga attcatcgcc aacacgaggc
     1201 cgggtacatc ggtggactac atcttcagga agggcatggc cgcatacgaa cgggccggct
     1261 atcccgagga gtggcatctc catcaccagg gcggaccgac gggctatgcg ggccgggact
     1321 atcgtgtcaa gcccggcatc caggagtccg tccagccgaa ccaggcattc gcatggaatc
     1381 cgacgatcac cggtaccaag tgtgaggaca cgattatcgt cctgaacgac cgcacggaga
     1441 tcatctcggc ctacggtgac tggcccatgg ttcccattga ggccgagtcc atgaccatcg
     1501 tacgcgagga tatccttgag ctgtagacgc tggtcgggga gcaaagactg ggaaatgatg
     1561 cagggctatg gtatactacc agtgcatcgt ctgacgcttt tcctcgaagg atgactcatt
     1621 gaaggaacga accgc
//
LOCUS       DATALS010000316         2913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_217488, whole genome shotgun sequence.
ACCESSION   DATALS010000316 DATALS010000000
VERSION     DATALS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217488"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..392)
                     /locus_tag="VHS06_07010"
     CDS             complement(189..392)
                     /locus_tag="VHS06_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987904.1"
                     /translation="MEFKGLYFKDIALLVAFILVTALSIPVVGWGAQAFAGHTALTLL
                     ALPVGLIGVLGLYLRSPRMVWTH"
     gene            complement(458..712)
                     /locus_tag="VHS06_07015"
     CDS             complement(458..712)
                     /locus_tag="VHS06_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987905.1"
                     /translation="MKEIKAPSIASVFVAGSLWWGAIAAASIAAAQPATAVLASAVAV
                     AQLWGASKFTGFAADAELRSSAVEVSEQRQIVSERAAKAA"
     gene            complement(889..1185)
                     /locus_tag="VHS06_07020"
     CDS             complement(889..1185)
                     /locus_tag="VHS06_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987906.1"
                     /translation="MVKNRMRSRATVGLLFASLWMGSVSAAAGVGVSGATSVTELFLR
                     LLFLAMGVAGWFLSFWYALSTLRPDEKAGEDRLVPVRVTVPVEPQQIPRRRRGL"
     gene            1558..2772
                     /locus_tag="VHS06_07025"
     CDS             1558..2772
                     /locus_tag="VHS06_07025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2987907.1"
                     /translation="MASFLSAVGFNFIFPLLPVYMHELTDSPTATVFWAGLAMAVTPL
                     ASAICSPFWGKLADRVGYRPMLLRALIGTCIAIGLMALPNAPWQLVLLRALSGGLGSF
                     QPMAMAALSSWTKPEDLSKVVSRLQMAQISGAIAGPLMGGLVTAAFGVRYSPVAGAIS
                     LTFGVVLVARWFHEPATRRTTLRGAGMKLSPMLLWLPMLTLVAVQFTDSSFNPILPLL
                     LGQVEPDAGKVAALSGLAASFNATSAAVAAGLAGQRLKHRVRKRTMVVGTVGVALLAL
                     AALRAPVPWGVVGLRILVGGMVNAIAVAAYSEGGLAVLPGQRGSAYGWLAASSSCGFA
                     ASPMVAGAMAAIDLRAVLGLDILFCLIATASWGFSTRTGAPVTGPVPLAVREQPSMEG
                     APQVAPKSGEKR"
BASE COUNT          539 a          960 c          877 g          537 t
ORIGIN      
        1 aaggcatgtt cacgcctgcc aggccgcgac cgtgacgctg cgctcgctcg cgtcacgtgc
       61 tcgctaccgt cgcgtgctct ggacccgtct tccttctatg acatactaag gcagccgatt
      121 cacgacaaaa agagaaggcc catgcccaac gggcacgggc ctcagagagg ggtttatctg
      181 acccctgatt agtgcgtcca gaccatccgc ggggagcgca ggtagaggcc caggacaccg
      241 atcaggccca ccggcagagc gagcagggtc agagcggtgt gtcccgcgaa cgcttgggcg
      301 ccccatccga ccaccggaat agacagtgca gttaccagta tgaatgcaac gagaagagct
      361 atatccttaa agtacaaacc cttgaattcc atttctagac tcctttgact ttcgggttca
      421 gccgttttgc cccttgccct ccaacacagg aggaagacta tgccgccttg gcggccctct
      481 cggaaacgat ctgccgctgc tcgctcactt ccacggcact gctccgcagc tcagcatccg
      541 cggcaaagcc ggtgaacttg gaggcgcccc acaactgggc aaccgcaacg gccgacgcta
      601 ggacggccgt cgccggctgg gccgctgcaa tcgaggctgc cgcgatggcg ccccaccaca
      661 gggaaccggc cacgaagacc gatgcgatac ttggggcttt gatctccttc atcaattagc
      721 acctttctgc ttttctacaa gccatctttt cttacggggc tactatctca cggatgatcc
      781 aaaaggtcca atgcctactt aggggtccag agataactca gaagttatca ttcggagggc
      841 gcgagaaagg gcgggcttcc gcccgcccta gtgatgcttc gatcgtcgct acagtcctct
      901 acgtcgcctc gggatctgct ggggctctac aggcacggtt acccgcaccg gcacaagccg
      961 atcctcccct gccttctcgt ccggccgcaa cgtggacagg gcgtaccaga aggagaggaa
     1021 ccaaccggcc acccccatcg ccaagaacaa cagccgcagg aatagctccg tgactgaagt
     1081 ggcgccagac acaccgacgc cggcggctgc agaaaccgat cccatccaca gggaggcgaa
     1141 gagaaggccg acggtagccc tactccgcat tctgttcttc accatgccct cccgagcttc
     1201 gctttatcta ctgagtcctc agccaatatt gcacaatcca ctcagtagtt ccaatgccgg
     1261 ctctatctgc catccataag gcaaggggta tgccgcgcga gaacaccacc accagtgctg
     1321 cgccaacgac gagtctaccg atggtagagg cgtacctccg tgtgcgcacc tcggcactgg
     1381 gcggacacgg aggtacgcac tcaccgtgcg ccggcagtat gcgcattcgc cccatcggac
     1441 gtcgcgatga aacactagaa ttgccggcgc atatgggttt taatagcggc tatggaaatg
     1501 atctctcgca cactcacagg tcgctcctct cccgataggg ctggattagc cctgattgtg
     1561 gcctctttcc tgtcggcagt cggcttcaac ttcatcttcc ccctcctgcc ggtgtacatg
     1621 cacgaactga cggattcccc caccgccacg gtgttctggg ccggactcgc catggccgtg
     1681 accccgctgg ccagtgcgat ctgctcgccc ttctggggga agctggcaga cagggtgggc
     1741 taccggccga tgttgctgcg ggcgctgatc ggcacctgca tcgcgatcgg tctgatggcc
     1801 ctgcccaacg ccccatggca actggtgctg ttacgggccc tctccggtgg actcggctcc
     1861 ttccaaccta tggccatggc agctctctcc tcctggacca agcccgaaga cctctccaag
     1921 gtcgtcagcc ggctgcagat ggcacaaatc tcgggtgcca tcgccggacc actaatgggt
     1981 gggctcgtca cggccgcctt cggggtacga tactccccgg tggcaggcgc catctccctg
     2041 accttcgggg tggtgctggt cgcccgctgg ttccacgagc ctgccacccg gcgaacgacg
     2101 ctgcggggcg ccggcatgaa actgagcccc atgctgctct ggctgccgat gctcacactg
     2161 gtggcggtgc agttcaccga ctcctccttc aacccgatcc tcccgctgct gttagggcag
     2221 gttgagccgg acgccggcaa ggttgccgca ctcagcggac tggccgcctc cttcaacgcc
     2281 acctccgccg ccgtcgcggc cgggctggcg ggccagcggt tgaaacatag ggttcgaaag
     2341 cgcaccatgg tcgtgggaac agtgggagtt gcgctgctgg cactggcagc cctgcgcgcc
     2401 ccggtaccct ggggagtcgt gggcctccgc atcctggtcg gcgggatggt gaatgccatc
     2461 gcggtagcgg cttacagcga gggcgggctg gccgtgctgc cgggccaacg gggctccgcc
     2521 tacggttggc ttgccgcctc cagctcctgc ggcttcgccg ccagcccgat ggtggccggg
     2581 gccatggcgg caatcgacct gcgcgcggtc ctcgggctgg atattctgtt ctgtctgata
     2641 gccaccgcga gttggggctt ctcgacacgc accggggctc cagtcaccgg accggtgccc
     2701 ctagccgtca gggaacagcc gtcgatggag ggcgccccgc aggtggcacc taagagcggc
     2761 gagaagcgct gacgggatcg gaggaagaca tgggacttca attgccgaac ctcgacggta
     2821 ggacgagaga gttgatgatc gaggagttgg aggcagacgt cgcagcgggt aggctgtggc
     2881 tcaaccctcg gctgagcagc aaaggtgagg ccg
//
LOCUS       DATALS010000317         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_217557, whole genome shotgun sequence.
ACCESSION   DATALS010000317 DATALS010000000
VERSION     DATALS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217557"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VHS06_07030"
     CDS             complement(<1..1068)
                     /locus_tag="VHS06_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987908.1"
                     /translation="MKRALIALVFAFVALLATDSLGLAQTSPQFRASFKALADMIPNV
                     VGAPTENEYWDPNGNVVQHTSTGMMVWRASDRWTAFTNGNITWIMGPYGLQSRSNSDL
                     YPWEVVPPGPTPAHQQTSPTTDQYNPYELPPIPTAPPTPVPAAATPVPPQPTATPAPA
                     SGPAPTYVPDVRAMDDESGGHGVTIKAYMQQMDGLWLAVWGWRPHKPTTIYLYNDGYR
                     MMYGISQITGQYFNQWDVDSTAANIAASLGSDRTNGGYAIFLNLAYQYGSDNWLDALK
                     ANLLQQYGNVMIQDMANNAGPDWFRIGMSQWMAYSNVTRTPAELGVTHYASLAKSRGT
                     LPSLYTLTNSWNATILSGGEAS"
     gene            1357..2565
                     /gene="thrC"
                     /locus_tag="VHS06_07035"
     CDS             1357..2565
                     /gene="thrC"
                     /locus_tag="VHS06_07035"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX2987909.1"
                     /translation="MPSFVCSLCGEVYSYSEHRQRCPKDGEPLDVRVDPGVARERVPG
                     AGLLHHWQGFLPFSTLSPDLDLGVGRTPLVKASRLKRRLGIEQLYFKNEGLNPTGSFK
                     DRGTVVGMQRALEMGVKAVGTVSSGNMAGSMAAFAARAGLECYVMVSSEIPPEKLGPI
                     GIYRPHLTLVEGDYGELYREALRIGEELGIYFIISDDPFRVDGQKTVVLEICEQLNWE
                     APDYIFVPVSSGGNISGVLKGLRELEERGMISRRPKVVGVQAAGSSPLARAFAAGKQK
                     FERFENPNTICHAITNANPPSGNRVLRWLEGGKYGHIVSVTDEEALDAQRLMAEEEGI
                     WGQPDSAVPLAAIIKSLSDGSISPDDRVVAIVTGNGLKDQSIFKSRPVETKRVQLAGL
                     ADLVKSLVRG"
     gene            complement(2737..>4350)
                     /locus_tag="VHS06_07040"
     CDS             complement(2737..>4350)
                     /locus_tag="VHS06_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX2987910.1"
                     /translation="ALGEAEAGGWWGPELKLAGFDAILITGKSPKPVYLWIHDGDVAI
                     RDASAIWGKGTGEAQEMIRRELGDAKIRVAQIGPAGEKMVRYACVLNELKHSNGRSGL
                     GAVMGSKNLRAIAVRGTGKPNLHDPARVDEVRKQIQGNYHRTPGDMHDLGTSGGIPFL
                     DMRGILPTRNFKEGSFEGADQISGQAMRDTILTGRGTCYGCYVACKREVKTGEPYNVD
                     PLYGGPEYETIGSLGSLCGISDLPAIAKGNEVCNALGLDTISVGTTIAFAMEAFEAGI
                     LTTKDTDGIELRFGNADAMLKLLPMIANRTGIGDLLAEGAKRAADKLGRGAEAMAMHA
                     RGQEFPMHEPRGKCSLAVGYAVSPTGACHMEAPHDTDFEMEGGAGLEAIAPLGVFEPV
                     ESTDIGPAKMRTFFPGQQVWNLYNCIGVCDFVGAPTGPMTLPMLVDYLNAVTGWNTSL
                     YELIKVGERSQTMARLYNIREGFTAEDDRLPERMFQGLENGALKGVGLDRKRFEDAKR
                     LYYEMAGWDPLSGVPTNGKLAELDLLWAI"
BASE COUNT          746 a         1296 c         1440 g          868 t
ORIGIN      
        1 gctcgcttca ccgcccgaca gtattgtcgc gttccagctg ttggtcagag tgtaaagcga
       61 gggtagggtg ccacgggact ttgccaggga cgcatagtgg gttacaccca gttctgctgg
      121 ggtacgagtg acgttgctgt aggccatcca ttgggacatg cctatgcgga accagtccgg
      181 ccccgcgttg ttggccatat cctggatcat cacgttccca tactgctgca gcaggttggc
      241 cttcaaggca tcgagccagt tgtcggagcc atactgatag gccaggttga gaaagattgc
      301 gtagccaccg ttagtccggt cgcttcccag ggatgcggcg atgtttgccg cggtgctatc
      361 gacatcccac tggttgaagt actgaccggt gatctgactg atcccgtaca tcatccggta
      421 gccgtcgttg tagagataga tcgtggtggg tttgtgcggc ctccagcccc acactgccag
      481 ccacaggcca tccatctgct gcatgtacgc cttgatggtg accccatgcc ctcccgactc
      541 gtcgtccatc gcccggacgt ctggaacata ggtgggtgcc gggccggatg ccggggcggg
      601 tgttgccgtg ggctgaggcg gtaccggagt agccgccgcg ggcacgggcg tcgggggtgc
      661 cgtcgggatc ggcggaagct catagggatt gtactggtct gtggtggggg aggtctgctg
      721 atgagcgggg gtgggacccg gcgggaccac ctcccatgga tacaggtcgc tgttcgacct
      781 gctctgcagc ccgtaaggcc ccatgatcca ggttatgttc ccgttggtga acgcggtcca
      841 ccggtcgctg gcacgccaca ccatcatccc cgtgctggtg tgctgaacca catttccgtt
      901 ggggtcccag tactcgtttt ccgtaggagc accgacgacg ttcgggatca tatccgccag
      961 ggctttgaag cttgccctga actgcggact ggtttgtgcc agtccaagac tgtcggtggc
     1021 caagagggca acgaaggcga agactaaagc tatcagggcg cgcttcattg cagttactcc
     1081 cttcttttcc gctatgtgag ggaaattggt gtttttcgcg atggggccca tagtcgagcc
     1141 cgttgctgat ctgacaggag atgcaggata caataagcca agtatagcag catcagctta
     1201 ccagacaagt ggctgttcta cacgtacgga ttgccggcgg agtgaggctg gtgtgctata
     1261 ctcgcgcctg tcctcagccc gcaggcgggc cgtcctggaa ggacggcgct acctctttgc
     1321 ctgcaccgcc agccttatcg gattggagga atcagcgtgc cttcttttgt ctgttccctc
     1381 tgtggagagg tctattccta ctctgaacac aggcagcgct gccccaagga cggcgagcct
     1441 ctggatgtga gagtcgaccc cggagttgca cgggagcgcg taccgggtgc agggctgctc
     1501 caccactggc agggttttct gcccttctcc accctttcgc cggacctgga tctgggagtg
     1561 gggcgcaccc cgctggtgaa ggcgagtcgc ttgaagcgga ggctggggat cgagcaactc
     1621 tatttcaaga acgaggggct gaatcccaca ggctcattca aggatagggg tacggtggtt
     1681 ggaatgcaga gggcgctcga gatgggtgtc aaggcagtcg gcactgtatc cagcggcaac
     1741 atggccggat cgatggcagc cttcgccgca agggcagggc tggagtgcta cgtgatggtc
     1801 tcctcggaga tccctccgga gaagctggga cccatcggca tatacaggcc ccacctgaca
     1861 ctggtcgagg gcgactacgg cgaactgtat cgggaggcgc tgcgcatcgg tgaggagttg
     1921 gggatctact tcataatctc cgacgatccc ttcagggtcg atgggcagaa gacggtggtg
     1981 ctggagatct gcgaacagtt gaactgggag gccccggact acatcttcgt gcctgtcagc
     2041 tcaggcggaa acatatccgg tgtgttgaag gggctgcgtg aactggagga gcgggggatg
     2101 atctcccggc ggccgaaggt ggttggggtg caggcggcgg ggtcgtcccc actggctcgg
     2161 gcctttgccg cgggcaagca gaagttcgag cgattcgaga accccaacac gatatgccat
     2221 gccatcacca acgccaaccc tcccagcggc aatcgggttc tccgctggct ggagggtggg
     2281 aagtatgggc acatcgtctc tgttaccgat gaggaggcgc tggatgccca gaggctgatg
     2341 gccgaggaag aggggatctg gggacagcca gactccgccg tgccgctggc cgccatcatc
     2401 aagtcgctct ccgatggcag catttccccc gacgatcgag tcgtggcgat agtaaccggc
     2461 aacgggctga aggatcagag catcttcaag agccgccccg tggagacgaa gcgggttcag
     2521 cttgccgggc tggccgacct ggtgaagtcg ctggtacggg ggtagcagcg ggcacataag
     2581 gcgtcggttg ccagaaagca cggcgttggg cggtgaatgc gggtggaggg gaggctttag
     2641 ccgagcgtta ctcacggttt cggctgaagc ctcccctcca ttcattgact ccgccgggcg
     2701 gttccggcta gagcctcgtt tccaggatag aacggactag atcgcccaga gcagatccag
     2761 ctccgccagc ttgccattgg tgggaacgcc ggagagggga tcccaccccg ccatctcgta
     2821 gtagagccgc ttggcatcct cgaaccgctt gcgatccaga ccgacgccct tgagagcccc
     2881 gttctccaac ccctggaaca tcctctcggg gagtctgtcg tcctcggcgg tgaagccctc
     2941 ccggatgttg tacagcctgg ccatggtctg ggatcgctca cccaccttga tcagttcgta
     3001 gaggctggtg ttccagccgg ttaccgcgtt caggtagtcc accagcatcg gtagggtcat
     3061 gggtccggtg ggcgcgccca cgaagtcgca cacgccgatg cagttgtaca ggttccacac
     3121 ctgctgcccc gggaagaagg tgcgcatctt agccgggccg atatccgtgg actcgaccgg
     3181 ctcgaagacc cccagagggg ctatcgcctc caggccggcg ccgccctcca tctcgaagtc
     3241 ggtgtcgtgc ggggcctcca tgtggcaggc gccggttggg ctgacggcgt agccgacggc
     3301 caggctacat ttaccccgcg gctcgtgcat agggaactcc tgccccctgg cgtgcatcgc
     3361 catcgcctca gcgccgcgcc ccagcttgtc ggctgcccgc ttggcaccct ccgccagcag
     3421 gtcgccgatc ccggtccggt tggcaatcat cgggagcagc ttcagcatcg cgtcggcgtt
     3481 tccgaagcgc agctcgatgc cgtcggtatc cttggtcgtg aggatcccgg cctcgaacgc
     3541 ctccatggca aaggcgatgg tggtgccgac cgagatggta tccagcccca gggcgttgca
     3601 cacttcgttg cccttggcga tagccggcag gtcggagatg ccgcacaggg agcccaaaga
     3661 cccgatggtc tcgtactcgg ggccgccgta gaggggatcc acgttgtagg gctcgccggt
     3721 cttcacctca cgcttgcagg cgacgtaaca gccgtagcag gtgccgcggc cggtgaggat
     3781 ggtgtcgcgc atggcctggc cgctgatctg gtcggcccct tcgaagctgc cctccttgaa
     3841 gttcctggtg ggcaggatgc ccctcatgtc caggaagggg ataccaccgg aggtgcccag
     3901 gtcgtgcatg tcgcccgggg tgcgatggta gttgccctgg atctgtttgc ggacctcgtc
     3961 caccctggcc ggatcgtgga ggtttggctt gccggtgccg cggacggcta tggcccgaag
     4021 gttcttggag cccatgacgg caccgaggcc ggagcggccg ttggagtgct tcaattcgtt
     4081 cagcacgcag gcgtagcgga ccatcttttc gcctgctgga ccgatctggg cgaccctgat
     4141 ctttgcgtcg cccaactccc ggcggatcat ctcctgggcc tcaccggtgc ccttgcccca
     4201 aatggccgac gcatcccgga tggcgacgtc gccgtcgtgg atccagagat agaccggctt
     4261 gggggacttc cctgtgatca ggatggcgtc gaagccggca agcttcagct ccgggcccca
     4321 ccagccgccg gcttcggcct cgccgagggc
//
LOCUS       DATALS010000318         6210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_257934, whole genome shotgun sequence.
ACCESSION   DATALS010000318 DATALS010000000
VERSION     DATALS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257934"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VHS06_07045"
     CDS             <1..194
                     /locus_tag="VHS06_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987911.1"
                     /translation="VEVVNANIGGNVDRQVEQRNENSEVSRIRFIMHMPTKTRAKIQQ
                     ENNALFAPQRGISIDDPYI"
     gene            complement(259..507)
                     /locus_tag="VHS06_07050"
     CDS             complement(259..507)
                     /locus_tag="VHS06_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2987912.1"
                     /translation="MTPRHRHRHTSPLPIGRGQRRWKPTRPIKERAVGTYRNLAHHAD
                     LDFSYINQLENGKRNPSLETLYKIATALEVPVKDLISD"
     gene            complement(865..1629)
                     /locus_tag="VHS06_07055"
     CDS             complement(865..1629)
                     /locus_tag="VHS06_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020460959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2987913.1"
                     /translation="MLTYPGTIALSERTLGFLAEVLQAHRERLGTWRKLSAQEQALLV
                     LAHLRNGDTYQRLATGFGVGLATAHRYIREAVAVLAGLGRSLTAAMWALAWTASNFAL
                     LDGTVVRTNRIRAYDRLYYSGQHKHHGVNLQGLTDPYGRLIWISDGLPGSTHDLSAAR
                     THGVFAVADLAQLYLYADKGYIGGDGLTLLTPYKGRNLANSYRDANRCHAAIRAHGER
                     GFAVLKNWKVFDRFRGCPRHIGTFARAVLTLEQYPK"
     gene            complement(1767..2009)
                     /locus_tag="VHS06_07060"
     CDS             complement(1767..2009)
                     /locus_tag="VHS06_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987914.1"
                     /translation="MDQLPVNHPLVLLVMASGPAQGLLWAPLVLLSTQQGSRTYVMLN
                     AYVLWPKPRQTWPKLVMIVGLYKKPKQLADTELFPP"
     gene            2189..2461
                     /locus_tag="VHS06_07065"
     CDS             2189..2461
                     /locus_tag="VHS06_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987915.1"
                     /translation="MIPAFAPAPRGTLVGRFPLAVHRDDGTTVWHAVLAFGDRAKLLQ
                     HRSDAGELAKGQEVSLIGYEHTRAQPTRDGGTKSVTEIYAVAVTRR"
     gene            complement(2626..2817)
                     /locus_tag="VHS06_07070"
     CDS             complement(2626..2817)
                     /locus_tag="VHS06_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987916.1"
                     /translation="MKKLPHIEKIVKQRVAAGIAGDAEFILWLKGDLEDASYSDKFKQ
                     QLLDELAEATRQKRTENIH"
     gene            complement(2922..3125)
                     /locus_tag="VHS06_07075"
     CDS             complement(2922..3125)
                     /locus_tag="VHS06_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987917.1"
                     /translation="MKYFKITRTWSVKAEDEAEALHQIAADPTEHLESEAVARTEYMK
                     KPKQETGWAAGIRNQLTGSNSQR"
     gene            complement(3346..4158)
                     /locus_tag="VHS06_07080"
     CDS             complement(3346..4158)
                     /locus_tag="VHS06_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2987918.1"
                     /translation="MVTYAATLDVPRPVVDYLARLLAAHRRRIGTPKGSRVLGCFRQA
                     VLVLRWFRERGCVHCLARNAGVSQATGYRYLHEGIDVLAGQAPELHEVLTRCRRDGCS
                     HVVLDGILIPTERLASTTEAGNDWWYSGKHRTFGGNIQVLAAPDGTPLWTSDVEPGSV
                     HDMAAARTHALPTLYWAASQLHLPTLADAGYHGAGAGVHTPVIKPAGRPYHALHVDNR
                     TYNQLLRGLRALSERAAAELTQRWRTLHYITLSPSRIGDIARAAVVLNNRWK"
     gene            4600..5433
                     /locus_tag="VHS06_07085"
     CDS             4600..5433
                     /locus_tag="VHS06_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017578586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2987919.1"
                     /translation="MVGYRALLDVPRELVAYLAGLLAAERRARGTRAGTRALSCWWQA
                     VFALVWFRERRDIPLTGRGLGISQATAYRYLAEAIDVLAARAPDLHQALQRVADEGWS
                     HVVLDGTVVACDRLAEKKTSKKGRLIDAWYSGKTSGFGGNIQAVLRPDGLPVWVADVK
                     PGSTPDLTAARPHVLGALYWAAAQLRLPTLADGGYHGTGIGVHTPVPNPADDGELDAD
                     TRAYNLLLRSLRCRGERGFALLTGRWRTLQHVTLSPNKIGHIAKAALVLTHFEHGYLT
                     A"
     gene            5995..>6210
                     /locus_tag="VHS06_07090"
     CDS             5995..>6210
                     /locus_tag="VHS06_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987920.1"
                     /translation="MSGAATPAPSGDDREAYPMPRPSEYQPYSFITVTQPPPQMVLLR
                     RGTGGEDGGSPPRSGSISEALKTPLRGG"
BASE COUNT         1133 a         1985 c         1938 g         1154 t
ORIGIN      
        1 ttgtggaggt tgtgaatgcg aacatcggtg gaaatgtcga cagacaagta gagcaaagaa
       61 acgaaaatag tgaggtaagt cgcattcggt ttatcatgca tatgcctaca aaaacccgag
      121 caaaaataca acaagaaaat aatgctcttt ttgcaccaca gcgtggcatt tctatcgatg
      181 acccttatat ataactctac ttattgttct cctttttctc acctcatggc ttcctggctg
      241 accaccaggc gcaggaagtc agtcagagat gaggtctttt actggtacct ctaaggcagt
      301 ggcaatttta taaagcgttt caagtgaagg atttcgtttc ccgttttcaa gctgattgat
      361 ataagagaag tccaggtcgg catggtgggc aagatttctg taggtaccca cagcccgttc
      421 cttgattggg cgggtaggct tccagcgccg ctgcccccta ccgatgggta aagggctagt
      481 gtgccggtgc ctgtgcctgg gcgtcacagg gttttcccaa acaccgcctt ttctatgggt
      541 aaaaacccca ggctgcgcaa aaacgcctga tactcctccg ttgaaaacag cggatcacta
      601 aagggcgtgg caatcgtccg ggtatccggg aagcgctgcc tgagctactt ctcaaagctg
      661 ttccacaagg ctgtcatgtg ggtatcctta aagagcggtt tatcccggac aaacagtcca
      721 aaaaaacact cctacagcac tagtgtccga tcaatcgggt agtaccatgc ctgcccgctg
      781 ccgattcgga tctcctgcaa cccctcctgg cgcgtgccaa gggacttgcc gatgaccgga
      841 tcagtgagct ttttccatat tgctttattt ggggtactgt tcgagggtga gaacggcgcg
      901 ggcgaacgtc ccgatgtggc gtgggcagcc gcggaaccgg tcgaagacct tccagttttt
      961 caagacggcg aagccgcgtt cgccgtgggc gcggatcgcg gcgtggcagc ggttggcgtc
     1021 ccggtaggag ttcgccaggt tgcgcccctt atagggggtg agcagggtga gcccgtcacc
     1081 accgatgtag cccttgtcgg cgtacaggta gagctgtgcg aggtcggcga cggcgaacac
     1141 gccgtgggtg cgggcggcgg ataggtcgtg ggtggagccg ggcagcccgt cggagatcca
     1201 gatcagccgg ccgtacggat cggtcagtcc ctgcaggttc accccgtggt gcttgtgttg
     1261 gccggagtag tacagccggt cgtaggcgcg gatccggttg gtgcgcacga cggtgccgtc
     1321 gagcagggcg aagttgctgg cggtccaggc cagcgcccac atggcagcgg tcaggctgcg
     1381 gcctagtccg gccagcacgg cgacggcctc ccggatgtag cggtgcgcag tggccagccc
     1441 gacgccgaag ccagtggcca gccgctggta ggtgtcgccg ttgcgcaggt gcgccagcac
     1501 cagcagagcc tgctcctggg cggacagctt ccgccaggtg cccagccgtt cccggtgcgc
     1561 ctgcagcact tccgccagga agcccagggt gcgttcggac agcgcaatgg tgccagggta
     1621 ggtgagcaac agagtcctcg gtcagatcgg aaggcttcag gcacctcctg atctaccggg
     1681 gactcctcca tggccagccg ccgccacggc aacagccggt caagctccga acaacctcac
     1741 aatgccggcg aaaaaaatcc catcaatcaa ggtggaaaaa gctcagtgtc tgccagctgc
     1801 ttgggctttt tgtagagccc gacgatcatg acaagcttgg gccacgtctg acgtggcttg
     1861 ggccagagga cgtatgcgtt aagcatgacg taggtgcggc tgccctgctg ggtggaaagc
     1921 agcaccagcg gagcccacaa cagcccctga gccggcccag aagccatcac aagcagcacc
     1981 agcggatgat tcaccggtag ttgatccacc cccttctacg gcgctcagac agccggctga
     2041 tacgaaacgc cgtcccggcc ccaccggcag tcctcccgac actcccccac ctgccatacc
     2101 ggacgcggca ccgccagtgg tagttgcacc gcggacagac cgacgacgga gcagcagccc
     2161 caggtgcagc tgaccggccg gctgggccgt gatcccagct ttcgcaccag ccccccgcgg
     2221 caccctggtc ggccgcttcc ccctggctgt ccaccgggac gacggcacca cggtctggca
     2281 cgccgttttg gcctttggcg accgggccaa gctcctgcaa caccgctctg atgccggaga
     2341 gcttgccaaa ggccaggaag tcagcctgat cggttacgaa cacacccggg cccagcccac
     2401 ccgggatggt ggcaccaaga gcgtcacgga gatctacgcc gttgccgtca cacgccggta
     2461 ggggcggcgg ggcggggcaa tgccttacaa gcgccccagc cctgctcgtc ccttccccga
     2521 gaggcttgac cggcaccgca gccccgccgc ccgatgctgc gggtttttcc tgattgatct
     2581 catgcggctc cctccaacat ggagcgtgga gggagcgaca caggatcaat gaatgttctc
     2641 agtcctcttc tgtcgcgtcg cctctgccaa ctcatcaagg agctgctgct tgaacttgtc
     2701 ggagtatgac gcatcctcaa gatcaccctt cagccacagg atgaactcgg catcccctgc
     2761 tatgcccgca gctacacgct gcttcacgat cttctcaatg tgtggcagct tcttcattct
     2821 catggacaca ccccctttcc cgagagactc agacgacagt ccccgttcgt ttaagctctc
     2881 gccacttccc ccatggttgt ggtggaggaa gccgtaagtg tttaacgctg actgtttgag
     2941 ccggtgagct ggttgcgaat cccagctgcc caaccggtct cctgcttggg ctttttcatg
     3001 tactcagtcc gcgccactgc ctcggactcc aggtgctcgg ttggatcggc agcgatctga
     3061 tggagtgctt ccgcttcatc ctctgccttg accgaccacg tccgggtgat cttaaagtac
     3121 ttcatgtgtc ccacctcctt tccgtgtctg gttaacacac acctcccgcc tggagcggga
     3181 agcgtctacc aactagagct caggtacttc ttccacctct gtgtccatga cattcaagat
     3241 gtgctcgctg tacttgctgt taagctcgat gatgtcaacg atctttcgtg cctcgtcctc
     3301 actcgctgca tcaagtgcga gatactgagg ttttctcagc ggtttctatt tccacctgtt
     3361 gttgaggacg actgcggcac gggcgatgtc gccgatgcgg ctggggctga gggtgatgta
     3421 gtgaagggtg cgccagcgtt gggtgagttc ggcggccgcc cgttcactga gggcgcgtag
     3481 gccgcgcagg agctggttgt aggtgcggtt gtcgacgtgg agggcgtggt aggggcggcc
     3541 ggcgggcttg atgactggag tgtggacgcc ggctccggcg ccgtggtagc cggcgtcagc
     3601 cagggtgggc aggtggagct gggaggcggc ccagtacagg gtgggcaggg cgtgggtacg
     3661 ggcggcggcc atgtcgtgga ccgagccggg ctcgacgtcg gaggtccaca ggggggtgcc
     3721 gtcgggggcg gccagaacct ggatgttgcc gccgaaggtc cggtgcttgc ccgaatacca
     3781 ccagtcgttg ccggcctcgg tagtgctggc gagccgctcg gtcgggatga gtatgccgtc
     3841 gagcacgacg tgggaacatc cgtcgcggcg acagcgagtc agcacctcgt gcagctcggg
     3901 ggcctgcccg gccaagacgt cgatgccttc gtgcagatag cggtagccgg tggcctggga
     3961 caccccggcg ttgcgggcca ggcagtgcac acagccgcgc tcccggaacc agcgcagcac
     4021 cagcacggcc tgccggaagc agcccaacac acggctgccc ttcggggtgc cgatacgacg
     4081 gcgatgcgcg gccaacaacc gggcaaggta gtccacgacg gggcggggga cgtcaagcgt
     4141 ggcagcatag gtgaccatgt gaagcctctg gctcctccgg atctgatctt ggcagaccag
     4201 ttcctaccag ggacttcaca cctatccggg tcgccacgct ggcgacggcc cacgccgcgt
     4261 gatgccacac cccaccagaa atcgctgaga aaacctcact gagtcttttt catcctcacg
     4321 tctgcccagc tcaccggcgc tgcccggttc ggccggagga gtctggtgca cgacgtccat
     4381 cgaggtcagc gagctcaggc cctgacccga cgtcgagctt cattggctcc gcctcggccc
     4441 tggaagccag gttgaaaagg ctcactgagg ctttctcagc aaggtcgaac gagtgaacgt
     4501 gtgccaggcg tagagggacg gcaggccccg gcatggacgt gaagccctcg gtagaagaag
     4561 aggtctcgct gaagatcgct tctcaccgag aggctccacg tggttggcta tcgtgccctc
     4621 ctcgacgtgc ccagggaact ggtggcctat ttggccgggt tgctggccgc cgaacgccgc
     4681 gcccgcggca cccgggccgg cacccgggcg ctgtcgtgct ggtggcaggc ggtgttcgcg
     4741 ctggtgtggt tccgcgagcg ccgcgacatc ccgctgaccg gccgagggct ggggatctcg
     4801 caggccaccg cctaccgcta cctggccgag gccattgacg tgctggccgc ccgggctcct
     4861 gacctgcacc aggccctgca gcgggtcgcc gacgaaggct ggtcgcacgt cgtgctcgac
     4921 ggcacggtcg tggcctgcga ccggctggcc gagaagaaga ccagcaagaa gggcaggctc
     4981 atcgacgcct ggtactcggg caagaccagc ggcttcggcg gcaacattca ggccgtgctg
     5041 cgcccagacg ggctgccggt ttgggtcgcc gatgtcaaac ccggctcgac ccctgacctg
     5101 accgccgccc gcccccacgt gctcggcgcg ctgtattggg ccgctgccca gcttcggctg
     5161 cccaccctgg ctgatggcgg ctaccacggc accggcatcg gcgtgcacac cccggtcccc
     5221 aatcccgccg acgacggcga gctagacgcc gacactcgcg cctacaacct gctcctgcgc
     5281 tcgctgcgct gccggggcga acgcggcttc gcgctgctca ctggccggtg gcgcaccctc
     5341 caacacgtca ccctcagccc caacaagatc ggccatatcg ccaaagccgc cctcgtcctc
     5401 acccacttcg aacacggcta cctcaccgca taactcgctg agaaaacctc acagtgtgac
     5461 ggcaaatctc atggtgtccc acctcctctc tttcactccc tttgtgtgtg ttgaagcttt
     5521 gcttccgctt aaattaaaag cggaaaagcg gaggtgaggg cagaaggacg accactgctg
     5581 tttcctatca agagtgggag ccgggctcga tccttccccc gaggaacgaa gagttcctct
     5641 cccaaccgtc cgaccttgat tctttacgaa cagccggaca ccgctggctc atgctcgcag
     5701 cgatccggct ctggccctgc ccagaggata ctgctcccat gccgcgcgga tagaagggca
     5761 ggcaccgccc gcccgcatgc tgcccgtgcg ggctcgccga ggcctccagg gtcaagggcg
     5821 gccgccgtga gacaaaagca agcctgcggc agacgtggcc gcctttgagg ctggaggggc
     5881 gagcccgtag aatgcgcggc ccttaggaag gccgagccgc gccagcggcc cgttcttggc
     5941 tctttcgcgc gtcagcgtct tactgcggcg ccgggcaagg agggtcaggg aggaatgtcc
     6001 ggcgccgcca ctcctgcccc ctccggagat gaccgcgaag cctatcccat gccccgcccc
     6061 agtgaatacc aaccttactc attcatcacc gttacccaac caccacccca gatggtgctc
     6121 ctgcggcgtg ggacaggcgg agaggacggg gggagtccgc cgcgaagcgg ctccatcagc
     6181 gaagccctca agactccgct tcgcggcgga
//
LOCUS       DATALS010000319         5735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_217735, whole genome shotgun sequence.
ACCESSION   DATALS010000319 DATALS010000000
VERSION     DATALS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217735"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..958
                     /locus_tag="VHS06_07095"
     CDS             257..958
                     /locus_tag="VHS06_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX2987921.1"
                     /translation="MEESGELSTLRGRNDDGAAPQKPLLRGWFHAFAAVGAVLVTIGL
                     LLQTLDDALRFISLLTFGLSMVELYSISAIYHICRWQGRCRTVFRSLDHANIFVMIAG
                     TYTPICVNVLSGWMRVGILVFIWTLALAGASSAVFTLRLPRWFSTALYLGMGWVALIP
                     LPTLLQLLPWQPIALLFAGGVLYSVGAVVYGLKWPNPFPRIFGFHEIFHLFVIAGCAA
                     FVAAIWIWVVPFPRA"
     gene            complement(1015..1419)
                     /locus_tag="VHS06_07100"
     CDS             complement(1015..1419)
                     /locus_tag="VHS06_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX2987922.1"
                     /translation="MALDLATAEKIAHAGLAHAKEMGIKVSFAVVDDAGNPMYMARMD
                     GAPFFAPNLAMGKAFVASAWRISSGEIEKRAQSAPYFYESAATASGGRMIIRQGALPI
                     VIGGVVVGAAGVSGGTSAEDEEVVAAGLKSIA"
     gene            complement(1481..2359)
                     /locus_tag="VHS06_07105"
     CDS             complement(1481..2359)
                     /locus_tag="VHS06_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2987923.1"
                     /translation="MIATVAAILLSIASWVTAAGVAASPPDAPDSLPPNAPARQLVTD
                     PDLSATLQDYLSQLDGNYGIAVENLVDGRSVLIHSDQPSPTASMYKLLVMYRAFEKDQ
                     QGEISMDDTITIIGDDMASAGPDEVPGVGSQLTVADALHAMITYSSNSAAWALARHMG
                     GWGEVFAAANELGMGLSAQDEGFVSSPADMLHFFELLYDQKLVGAEASRQMMQLLLGQ
                     TINDRIPALLPSEVPVAHKTGDLEEVRNDGGIVLASGSDYIIVIMANNVNPVQAVEAE
                     SLISKMVYDRYGTSNG"
     gene            2690..3412
                     /locus_tag="VHS06_07110"
     CDS             2690..3412
                     /locus_tag="VHS06_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2987924.1"
                     /translation="MRVDENVCAGCWECVPYCPMGAILPEDGVVRVDEEQCVECGTCL
                     RAAPCPSGAIYEPESTFKWPRSVRKVFSDPKAVHEGTGVPGRGTEECKTNDVTGRTKR
                     GRIGLAVELGRPGVGTDFHDVEKVSTALAALGVHFEPCNPLSSLMVDMTSGKIKDEVK
                     GERVLSAIVEIDAGIDQLKAVLETVKRVTAELNTVCVVDLISRFDPDGSIPVLPILKE
                     MGIEPRPNAKINIGTGKPLCNL"
     gene            3429..4058
                     /locus_tag="VHS06_07115"
     CDS             3429..4058
                     /locus_tag="VHS06_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987925.1"
                     /translation="MTHSLHRMGAAEDLHDDYVLMVTPAIGFNHEGKKEALQKVVDIV
                     WEVGPNNIASIEATGTIFTGTTKDDIKKNIHDKGRVRCCFSSREKIEEVVRRLKEADL
                     GLSIIVSGLIDEVVPMAKGLGCEPHTINLSLGIHGNVGCLPENGALEITTMCGHGLVA
                     TKLARAMIKEVKAGRKTLDEAAIKLAQPCFCGIFNTERAKQILLAADVQ"
     gene            4432..5655
                     /locus_tag="VHS06_07120"
     CDS             4432..5655
                     /locus_tag="VHS06_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987926.1"
                     /translation="MRAMRIGTLRWAVGAFSSVMGTMMLVAPHQYRLPEYASIRHLFP
                     LVGCIFLGAGIALLVVAVLRTRGYVSLLSHLLAAASLLSLSAGFFTTGIWSGTISYSI
                     LGVATVGAYFLARADDGGTARREGDLFAAAIGLSTAVEGAWFLFFPGVFTADFYRISL
                     PNLSWYGAAFLVSGLALLFVQLFPSLPPAATWAAHLSVGASFYSFGLPHLSNGTSTWT
                     GAGYYGGFGLLIAFLPYMARQWERVDLDSFRTRLALALAVSVAIPLVLMGALESEQGE
                     QSSTEEALARQRLQADTLSQLFVDHFSRYKSVAVALASQPDLLTMSDEARRMLLGNLK
                     NALPGPVALAILDVNGKEIARSDDAPLGLPDCRKWTSSRVKARRATVCALRTPKWRSR
                     PDTSTLTPLTSPCWL"
BASE COUNT         1029 a         1689 c         1784 g         1233 t
ORIGIN      
        1 ggtgtatctc caccttgaga gccattctgt gcaacattgc ttggacctcc ccctgtccgc
       61 ggccgagacg tagccggcca aacattggtg gttgccgagt tggggacatt tcccccggtt
      121 cgcgattccc cccggcttac atctacctcc caaccatcga gctcactgat ggcgcaagat
      181 ccgttctctg gtagaatggc gtcggcaatc gattgtggcc ccggacaacg ggacttgacg
      241 ttggggaggg gttccggtgg aagagtcagg cgaactctcg accttacggg ggaggaacga
      301 cgatggcgcg gctccacaga agccgctgct gaggggatgg tttcacgcct tcgctgcggt
      361 cggggcggta ttggtgacaa tcggtctgct gttgcagacc ctggacgatg ctctccgctt
      421 tatctcgctg ctgacctttg ggctgagcat ggtggaactg tattccatca gcgccatcta
      481 ccacatctgc cgctggcagg gcaggtgccg aacggtgttc cgttcgctgg atcacgccaa
      541 cattttcgtc atgatcgccg gcacatacac gccgatttgc gtcaacgttc tcagcggttg
      601 gatgagggtg ggcatactcg tgttcatctg gacgctcgcc ctggcgggcg cctccagcgc
      661 agtcttcacg ctgcgtctcc ctcgctggtt ctccaccgcg ctctatctgg gcatggggtg
      721 ggtcgcgctg ataccgttgc cgaccctact ccagttactg ccctggcagc cgatcgcatt
      781 actgttcgcc ggaggggtgc tctactcggt gggggcggtg gtgtacggtc tgaagtggcc
      841 caacccattt ccgcgcatct tcggcttcca cgagatattc cacctgttcg tgatcgccgg
      901 gtgtgccgcc ttcgtcgcgg ccatctggat ctgggtggtg cccttcccga gggcgtgacg
      961 cggggacacg tctccgcgtc tccgcgtctc cgtgtccccg tgtcctcgta gtggctaggc
     1021 gatggatttg agacccgcgg ccaccacctc ttcgtcctct gctgaggtcc caccacttac
     1081 acccgcggct ccgaccacca cgccgccgat gacgatgggc agggcgccct ggcgaatgat
     1141 catcctgcct ccggatgcag tagccgccga ttcgtagaag tagggggccg actgtgccct
     1201 cttctcgatc tccccgctgc tgatgcgcca cgccgaggcc acgaacgcct tgcccatggc
     1261 cagatttggc gcgaagaagg gcgccccatc catcctggcc atgtacatcg ggtttccggc
     1321 gtcgtctacc acggcgaagc tgaccttgat acccatctcc tttgcgtgag ccagcccagc
     1381 gtgagcgatc ttttccgcgg ttgccagatc cagcgccatc ttctcctctc ctccctacac
     1441 tatcctctct cttggacacc gatcgtcgaa cggcgtgaga ctatccgttg gacgtcccgt
     1501 accggtcgta caccatcttg gagatcagcg actctgcctc gactgcctgc acagggttta
     1561 cattgttggc catgatcacg attatgtaat cgctgccact ggccaggacg atcccgccgt
     1621 cgttcctgac ctcctccagg tcgccggtct tgtgtgcgac cggtacttcc gatggaagca
     1681 gcgccgggat gcgatcgttg atggtctgac cgagcaacag ttgcatcatc tgccggctgg
     1741 cctcggcgcc gaccagtttc tgatcgtaga gcagttcgaa aaagtggagc atgtcggctg
     1801 gcgaactgac gaacccctcg tcctgggcgc ttagtcccat gcctagctcg ttggccgcgg
     1861 cgaagacctc cccccacccg cccatgtggc gagcaagcgc ccaggcagcc gaattgctgc
     1921 tgtaggtgat catagcgtgc agcgcgtcgg ctaccgtcag ttgggaccca actccgggta
     1981 cctcatccgg gccggcagat gccatgtcgt ctccgatgat ggtaatggtg tcgtccatcg
     2041 agatttcgcc ctgttggtcc ttctcgaagg ccctgtacat caccagcaac ttgtacatgc
     2101 tggccgttgg actcggctga tcggagtgta tcagcacaga gcgtccgtcg accagattct
     2161 cgacggctat gccgtaattg ccgtccaatt gagaaaggta gtcctgcagt gtggcggata
     2221 gatcggggtc ggtgaccagt tgccgggccg gagcgttggg gggcagcgaa tcgggggcat
     2281 caggtggaga cgccgcgacc cctgcagccg tgacccagga tgcaatcgac agtaatatcg
     2341 ctgcgacagt tgctatcaac gctgcttcca cctcttcatt gtggatgata ctacccggat
     2401 ctgttcagcc gggcaagaca ggatctcgaa aggcaggatc gatgccgaga agggaaccac
     2461 caagaagctg tacatgaagg ccatcctctg ctatccttgc gacatcagct actatcctga
     2521 aggttcctag tctcgtgtca cacgtgcccg gtatgtgttt gtgtggcaag agaaagtcca
     2581 gacatttcta tgtccgattg tatctctttg cctccggacg tcgcgccacc atcgggcaca
     2641 gccaggccga cccatcgctt actcatcact tatgagaaga ggagtctgaa tgcgcgtcga
     2701 cgaaaatgtg tgtgccggtt gctgggaatg tgtcccctat tgccctatgg gggcgattct
     2761 tcctgaggat ggggtagtca gggtcgatga ggagcagtgc gtggagtgcg gcacctgcct
     2821 gcgggctgct ccctgcccga gtggagccat ctatgaaccc gagtccacct tcaagtggcc
     2881 ccgttcggtc cgcaaggtgt tcagcgatcc gaaggcggtg cacgagggga cgggggtgcc
     2941 gggtcgcggg acggaggagt gcaagaccaa cgacgtgacc ggcaggacca agcggggacg
     3001 aatcggtctg gcggtggagt tgggcaggcc gggggtgggg actgacttcc atgacgtgga
     3061 gaaggtctcc acggcccttg ccgccctggg agtccacttc gagccgtgca accccctgtc
     3121 gagcctgatg gtggatatga ccagcgggaa gatcaaggac gaggtgaagg gggagcgggt
     3181 gctgtccgcc atagtggaga tcgacgcggg gatcgatcag ctgaaggcgg tgctggagac
     3241 ggtgaagagg gtgacggccg agttgaacac ggtgtgcgtg gtggacctga tcagccggtt
     3301 cgatcctgat ggaagcatac cggtgctgcc gatcctgaag gagatgggga tcgagcccag
     3361 gccgaacgcc aaaatcaaca taggcacggg caagccgctg tgcaatctgt agccggaagg
     3421 atatagagat gactcactca cttcatcgaa tgggagcggc ggaggatctg cacgacgact
     3481 acgtgctgat ggtgacgcct gcgatagggt tcaaccacga ggggaagaaa gaggcgctgc
     3541 agaaggtcgt ggatatcgtc tgggaagtgg ggccgaacaa catagcgtcg atagaggcga
     3601 cagggacgat cttcactgga accacgaagg acgacatcaa gaagaacatt cacgacaagg
     3661 ggcgggtacg ctgctgcttc tccagtagag agaagattga ggaggtggtc cggcggctga
     3721 aggaggccga tctggggcta tcgatcatcg tgagcgggct gatcgacgag gtggttccga
     3781 tggcaaaggg attggggtgc gagcctcaca cgatcaacct gtcgctgggg atccacggca
     3841 atgtgggctg cctgccggag aacggagctc tggagattac gacgatgtgc ggccacggcc
     3901 tggtggcgac gaagttggcc cgggcaatga tcaaagaggt gaaggcgggg cgcaagacgc
     3961 tggacgaggc tgcgatcaag ttggcccagc cctgcttctg cgggatcttt aacaccgagc
     4021 gggccaaaca gatcctgctc gccgccgacg ttcagtagcc ggttgcgctg aggcgcttgc
     4081 ggcctagatt ggcgagaaac catgctcggg ggccgggtcg gagtgctccg acgcggccct
     4141 cgagcccttg ttccaattgt tctatgtaga taatctctat ctacaggtgg cgaacgctgt
     4201 tgacatccat tgcaccgacc ttcaagatta tagtgcccta gtctccaccc ggaggggctt
     4261 tgtgtgcttt tcttcctggc gacaaggctt ctgagtattc gtttcctgta tgtccaagca
     4321 acatccctga tccggtgata gtccccacgc tgccgccgat agtgggctca tcccatcctc
     4381 ggtctggggc gtggtgtcgc ggaacgggac acctgccatg gaaacgaccc aatgcgagct
     4441 atgaggatcg gcaccctgcg atgggcagtg ggtgccttct ccagcgttat gggcaccatg
     4501 atgctggtag cgcctcatca gtatcgtctt cccgagtatg cctccattcg ccatctgttc
     4561 cctttggtag gctgcatctt ccttggggcc ggcattgcac ttctcgtggt tgctgtcttg
     4621 aggactcgtg gttacgtatc tctgctttcc caccttctgg ccgcggcttc gcttctttcg
     4681 ctctctgcag gcttcttcac gaccggcatc tggagcggga ccatcagcta ctcgatcctt
     4741 ggggtagcca cagttggcgc gtatttcctt gcccgtgccg atgacggcgg gacagcccgc
     4801 cgagagggtg atctgttcgc ggcggcgatc gggctgtcca cggcggtgga gggtgcctgg
     4861 ttcttgttct tccccggggt ctttaccgcg gatttctacc ggatctcgct tccgaatctc
     4921 tcctggtatg gggcggcctt tctcgtttcg gggctcgccc tccttttcgt gcagcttttc
     4981 ccctcgcttc ctccggcggc gacctgggca gcgcatctgt ctgtgggggc ttccttttat
     5041 agcttcggcc tgccgcacct atcgaacggt accagtacct ggacaggcgc gggctattac
     5101 ggcggttttg gcctgctcat agcctttctt ccctatatgg cgagacagtg ggaacgggta
     5161 gatctggatt ccttcaggac acgcctggcc ctcgccttgg cggtatctgt tgccataccg
     5221 ctggtgctga tgggggcgct ggaaagcgag caaggcgaac agtcatccac cgaggaggcg
     5281 ctggctcggc agcgacttca ggcagacacc ctctctcaac tgttcgtcga tcacttctct
     5341 cgatacaagt cggttgccgt tgcgctcgcc tcccagcccg acctgctcac gatgagtgat
     5401 gaggctcggc ggatgctgct cggcaacctg aagaacgccc tgccgggccc agtcgcgctg
     5461 gcgatcctgg atgtcaatgg aaaggagatt gcccgcagcg acgatgcacc cctcggtctg
     5521 cccgactgca ggaagtggac gtcgtctcga gtgaaggcac gacgagcgac cgtatgtgca
     5581 ctcagaacgc caaagtggcg ctcccgccca gacaccagca cgctcacacc actcaccagc
     5641 ccatgctggc tgtagatcgg aggacaggag aagcgagtct ccgcggaaag atcctcgact
     5701 atcacaggtt cgtccgccat caaggtgtaa ccggc
//
LOCUS       DATALS010000320         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_106361, whole genome shotgun sequence.
ACCESSION   DATALS010000320 DATALS010000000
VERSION     DATALS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106361"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VHS06_07125"
     CDS             <1..690
                     /locus_tag="VHS06_07125"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEX2987927.1"
                     /translation="PLGRVLDGIVHQEGSDDYSGQYPYSFSPARQAFDRWFLHCLQSA
                     VSLTFRHWLLLANLVNGLTLVGAVAAPLLMAYGWESAGSALFTLYSLICAQNPEHSYF
                     LLGHQLAMDQRMMAIYGASLAAGLAFVPMRGRLRPIPWRLFGLMALPMAMDGFTQLLG
                     WRHSSWELRTVTGGLFGVAVVWLMYPFLDRQLPPLGPRSGQQARIHHAEDHRNVGYHQ
                     EDRHEGGMNLH"
     gene            complement(595..1113)
                     /locus_tag="VHS06_07130"
     CDS             complement(595..1113)
                     /locus_tag="VHS06_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6803 family protein"
                     /protein_id="HEX2987928.1"
                     /translation="MGMGSTVQQAGTTAANAMSGMTHYMQLLSENQPWNLILFMAIPV
                     IFAEFIAITELYILFTRNYASVWRRLNGIASIIVGFYFTGVFVYLFTNAVIPLTTQGG
                     WRGLADVVAVGFYLAGIVPLGGIALVELRTWLSGKEASASMKVHAAFVAVFLVVAHVA
                     MIFGMVDPRLLA"
BASE COUNT          319 a          485 c          491 g          351 t
ORIGIN      
        1 cccctcgggc gtgtacttga tggcattgtc caccaggaag gatcggacga ctactcagga
       61 cagtacccgt actccttctc accggccagg caggctttcg atcgttggtt tctccactgt
      121 cttcagtcgg ccgtcagtct cacgttccgg cactggctcc tgctggccaa cctggtcaac
      181 ggacttaccc tggttggggc cgtcgcagcc ccactactga tggcatacgg gtgggaatcg
      241 gcgggcagcg cactgttcac gctatacagt ctgatctgcg cccagaaccc cgaacactcc
      301 tatttcctct taggccacca gctcgccatg gatcagcgaa tgatggccat ctatggagca
      361 tcgctggcag ccgggctcgc atttgtgccg atgagggggc ggctgcggcc cattccgtgg
      421 cgtctcttcg gattgatggc cctacccatg gcgatggacg gctttaccca gctactgggg
      481 tggcgccaca gtagctggga gttgcggacc gtaaccgggg gattgtttgg agtggccgta
      541 gtctggctga tgtatccctt cctcgatcgc caactgccac ccttgggtcc gcgttcaggc
      601 caacaggcga ggatccacca tgccgaagat catcgcaacg tgggctacca ccaggaagac
      661 cgccacgaag gcggcatgaa ccttcattga cgcgcttgcc tccttgcccg agagccaggt
      721 ccgcagctct accagggcga tgcctcccag aggcacaatt ccggccaggt agaagccaac
      781 cgcgacaacg tccgccaatc ctctccaacc cccttgagtc gtgagcggga tcaccgcgtt
      841 agtgaacagg tagacgaaca cgccggtgaa gtagaagccg acgatgatgg aggcgatccc
      901 gttgagccgc cgccagacgg acgcgtagtt gcgggtgaac aggatgtaga gctcggtaat
      961 cgcgatgaac tccgcgaaga tcacagggat agccatgaat aggatcaagt tccacggctg
     1021 gttttcgctc aagagctgca tgtagtgggt cataccggac atggcgttcg cggctgttgt
     1081 gccggcttgc tgaaccgtac tacccatacc catatcgtgc atcgtagcac ctccttggct
     1141 tagaaggatg ccatatagcc cttagaatgc gctgagagcg tgcttagtgt gtgttgagag
     1201 tttgggtttg atgaggtgtg tcggcggggc aatggaagcg acacgtgaac gccgtcaggg
     1261 atatgccgga gaatgatcgc ggtcactatt ctacgggagg agtgtctgta ggtatgccgc
     1321 ggtccccgta tttctcgtct atgcgggctc ggatctcctt gatggtcagt ccctgacctt
     1381 tcaactgtcc ggcatcctcg gcctcgtcca agcagatctg gcagttggcg ccgtgggatc
     1441 tgaactcccc cttgtcgtcc aggaagcagt ctcgcaggct cttgtactgc tcgtcctggc
     1501 cgcagccgcc ccctcagcgg cacaaatacg agcccggctg ctagcgatgc gccatagatg
     1561 gccatcattc gctgatccat tgcgagctgg tggcctaaga ggatgtagag ctcggtaatc
     1621 gcgatgaact ccgcgaagat cacagg
//
LOCUS       DATALS010000321        11925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_218132, whole genome shotgun sequence.
ACCESSION   DATALS010000321 DATALS010000000
VERSION     DATALS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218132"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2656
                     /gene="cydC"
                     /locus_tag="VHS06_07135"
     CDS             <1..2656
                     /gene="cydC"
                     /locus_tag="VHS06_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEX2987929.1"
                     /translation="SLWERPPEGRVRAPRVPVLNGVSFEIQPGERVALVGPSGAGKST
                     LVDLLLDFLPPDSGRILVNGTPLSRLPAAEWREQVSWLPQTPYLFHASIETNLRLARP
                     DATREEILEAARLAGAHEFITQLPEGYDTVVGERGERLSGGQAQRIALARAFLKDAPL
                     LILDEAAAQLDVENEALLEESLDCIMEGRTVLLIAHRLGTARRADRVVVLAEGRVAEC
                     GPHEELRRRGGLYSRMVQAAGEGDAETGRHRDTGTRGHGDTGGPHPPFGRPLPEGEGR
                     GEGTQHSAPSTQHSSLHTQNSEPRTQNPEPRTLAGLLGLLRPLWGWMALSALLGFCTV
                     AAGVGLMATAAFLIASAALHPSVADLAVPIVGVRFFGLSRGVFRYLERYCSHYATFTL
                     LARLRVSCYRALEPLAPAGLMGQRGGDLLSRVVADVETLQNLYLRVVAPPVTALLVVA
                     AMVIFLGAFAPVLILPLIATALAAGLGVPLLSGLASRGLGAGSTLLRSELNVQLLDGL
                     QGMADLVVFGRDRHQLKLVTHTNRQLAAVQRQATVVSGLQSGLLSLLDGLGMWGVLAI
                     ALSLVAAGRLDGVYLPVVGLATLACFEVLAPLPLAAQQIEGSLAAGRRLLEIASAEKM
                     EPQATANWDHHSLTPSLSIRDLTFSYRQGQPPALDGVNLQLEPGRSLAIVGRSGAGKS
                     TLLHLLLRFWDYEEGSVLLGGRELREIPPEESRRLMAVVPQRTHLFNLTIRENLMLAR
                     PNATEQQMVEAARQARLHDFVASLPLGYDTFVGEQGIRLSAGERRRIAIARALLKDAP
                     ILLLDEPTAGLDSLTEKEVMDTLRGVMAGRTTLLVTHRLVGLQEMGEILLFQNGRVVE
                     RGTQQQLLSQDGHYRRSWELQRQLLPQE"
     gene            3188..4060
                     /locus_tag="VHS06_07140"
     CDS             3188..4060
                     /locus_tag="VHS06_07140"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX2987930.1"
                     /translation="MDYVRLTKPGSVGLLLLTALAGMTAVGSGLPSLSLVVATLAGGA
                     LAAGGSNALNCYLDRDLDRLMPRTANRPLPSGRLAPLKAAAFGILLCLLSFVLLAATV
                     NLLAALLAMAGMIYYVVVYTLWLKRRSPWNVVVGGGAGGLPLLVGSAAATGEISPWAL
                     ILGAVIFLWTPPHFWSLALLRRDEYGRAGLPMMPVVRGSLETRRQILLYSMFLVGLSL
                     LVPVMGFTGSGPLFVAAILGTVMLMLALRLLRHESPRAARQLYRYSVLYLALLLSTLI
                     LDRAGGLPALLGTS"
     gene            4087..5151
                     /gene="coxB"
                     /locus_tag="VHS06_07145"
     CDS             4087..5151
                     /gene="coxB"
                     /locus_tag="VHS06_07145"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX2987931.1"
                     /translation="MRPGSHAHLKAVPLTAMVVLGAAVLAGCQQAPAAIFRPESPGAT
                     AIHNLSLYLLAVAGVALVGVEVTLFLAVARFSNRPEEEAVQTHGNLKLETGWTAATAI
                     VVFITLGLTVNTMVDVTANIAESPPSLASAMPLQSAWPGDTLPMRVVGHQWWWTFEYP
                     QQGIVTANEAHVPVGDTVRAQLESGDVIHSFWAPRLGGKTDMIPGQINYTSFLAATIG
                     SYTGECAEFCGVEHAHMGFTIVVDSPADFSDWVAGQQAPAASAGTDAERAGYQTFTRS
                     CGVCHTINGTEARGKSGPNLTHLASRQMLAANTLENNPTNLARWIQDPQGLKPGNLMP
                     NQKLDQQQISDLVAYLSILK"
     gene            5177..6820
                     /gene="ctaD"
                     /locus_tag="VHS06_07150"
     CDS             5177..6820
                     /gene="ctaD"
                     /locus_tag="VHS06_07150"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX2987932.1"
                     /translation="MSSTADSLRGRNWDDSGIWSWITTTNHKRIGVLYLTTSFVFFLA
                     AAAMAMLMRTQLIKPDNDFLSPDVYNQIFTMHGTTMVFLFGMPVLAGFGNYIVPLMIG
                     ARDMVFPRLNAFSYWTYLAGAILLYSSFVFGGAPNAGWFSYAPLTEMTYSQTAGMDYW
                     SLSIIMLGMSSIAGAVNIFVTILTLRAPGMGFNRMPLFVWATFVNQFIILVALPSLTA
                     AAIMLYLDRHYGTSFFNPATGGDPLLWQHLFWFFGHPEVYILILPAFGIISEVIPVFA
                     RKPIFGYSFIAYSSVAIGILSFAVWAHHMFAVGMSLAADAVFAFTSFTIAVPTAVKIF
                     NWLATLWGGALRLKTPLLFAMGFIGLFVIGGLSGMAVAVVPFDWQVTDSYFVVGHLHS
                     VLFGGTAFGVFAGIYYWFPKITGRMMDERLGVLSFALLVVGFLFTFQPFHILGLLGMP
                     RRIYTFAGNMGWNELNLIGTLGAYVIAVAAVVFLWNLMASLRNGRVAGPDPWDAWTLE
                     WATSSPPPVHNFDRMPEVRSRRPLWDEKHPERADYLVRH"
     gene            6962..7198
                     /locus_tag="VHS06_07155"
     CDS             6962..7198
                     /locus_tag="VHS06_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987933.1"
                     /translation="MGDIETEGPAGTEPGAEPNGHKVGEHPAVSEHSPWPVLLSLGIL
                     IVAIGLLYQPAIVAVGAAVCVAAVIGWLWQPWVS"
     gene            7217..7801
                     /locus_tag="VHS06_07160"
     CDS             7217..7801
                     /locus_tag="VHS06_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEX2987934.1"
                     /translation="MIARTMGHGPQPLATRFDPTIVGLWVFIATEALFFGMLMGSYLY
                     LRVRLGSWPPPGTPERDLFVPLLNTVVLLSSGLVMHGAHDSIRRGDRSVLQGGIVATM
                     FLGAAFLGGQAYEYTHAGFGLSSGLLGSTFFTLTGFHGAHVFAGLVFLALILWRASKG
                     YYTANHHVGVEASALYWHFVDAVWVVLLTILYLI"
     gene            8019..8525
                     /locus_tag="VHS06_07165"
     CDS             8019..8525
                     /locus_tag="VHS06_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX2987935.1"
                     /translation="MEKKLALLTAAILVPTIIMASACGGGAAPAPTSAPAAPAATKAP
                     AATSAPAATKLAATPAGAATPAGAATVAAGGSTTAPGATTTTPAASASAAGDVTAGKA
                     IFDQNCNQCHPNGDKGAGPALRGRNLSADRIKNQVRNGGGGMPAFPASRITDQQLNDL
                     VAYVQSLQ"
     gene            8554..8991
                     /locus_tag="VHS06_07170"
     CDS             8554..8991
                     /locus_tag="VHS06_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX2987936.1"
                     /translation="MPRIGWAVWSLTLMLAAAALTGCQSGRASGAAASVAPSAATPAS
                     KTVWNGHYAQDIQPIFNDHCVTCHGPNRADNGLRLDSYEGVMKGTNHGPVVVAGDPDH
                     SALISVVQGSVEPSIRMPHGGQKLTEQDVQNLRLWIAAGASSN"
     gene            9032..9556
                     /locus_tag="VHS06_07175"
     CDS             9032..9556
                     /locus_tag="VHS06_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX2987937.1"
                     /translation="MQSETAEKQQIERRTFLGYVIVAVGAFITAVLGIPLIGSLIAPA
                     LRGRETNWVTVGSTMDFSVGQPKAATVTVTTKDGWITQQESKGVWVIKQSETSFVVYN
                     GRCTHLGCAYNWSPPQQEFDCPCHGGRYSLDGKVLGGPPPRPLDTLQWRVDQGNLVIR
                     YEDFRLGVPQKEVA"
     gene            9553..10734
                     /locus_tag="VHS06_07180"
     CDS             9553..10734
                     /locus_tag="VHS06_07180"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX2987938.1"
                     /translation="MTTTAPSPEPKPEGRVGQVSGWLLERAGAMGIWESFFARKVPLG
                     VGWLYTLGFASMFAFTVQAATGIFLAVYYSPSPDHAYDSIQFIMFQVPFGAVVRGFHH
                     WMASVMVVLVILHMATVFVLGAYKYPRELTWMVGVGLLVVTLGFAFTGYLLPWDQKAY
                     WATVVGTNIPGTLPVVGDLILRIMRGGAQLGTLTLTRFYSFHVLLLPASLAALLGAHL
                     YLIVHHGVSVPPWLWDSVAPRVRAVLGHREEAERAATREEAYHRRYEQFKREGHPFWP
                     DVIVQDTLAAAVVLLCTIGLMVTMGVPTEEPADPTNTAYVPRPEWYFMFLFQALKYFP
                     GNLEWVGAAVLPGVVMLAILLLPVYDRGPNRAPWKRPLAMTAGAAMAAIVGYLTYVAY
                     Q"
     gene            10939..11382
                     /locus_tag="VHS06_07185"
     CDS             10939..11382
                     /locus_tag="VHS06_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX2987939.1"
                     /translation="MKSVHLIGLGLVVGSLALGTMISVMTFRWQVETFPSTGPVVAAA
                     AGPAGAAMPAHSSTDAAKAKALFDSKCSSCHSIGGGKKMGPDLKGVATRRSADWIAQF
                     IMAPDQVIAAGDQAATQMVKEYGMPMPNLGISKEDAATLTAYISQ"
     gene            11643..>11925
                     /locus_tag="VHS06_07190"
     CDS             11643..>11925
                     /locus_tag="VHS06_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2987940.1"
                     /translation="MAYDRDETVKHILAIEKHIYQWVQLGGIPSWAETDLTMPQLKVL
                     ILVDRHHPMPTSQVARTLGMTLSTATGVVDRLVSQELIERLEDPADRRVV"
BASE COUNT         2145 a         3794 c         3784 g         2202 t
ORIGIN      
        1 ctccctctgg gagaggccgc ccgaagggcg ggtgagggcc ccccgtgtcc ccgtcttaaa
       61 cggcgtctcc ttcgagatcc aacccggcga gagggtcgcg ctggtggggc ccagcggcgc
      121 ggggaagtcg acgctggtgg acctgctcct ggacttcctg cccccggata gcggccggat
      181 ccttgtgaac ggcacgcccc tctcgagact gccggcggcg gagtggcgtg agcaggtatc
      241 gtggctgccg caaaccccct acctgttcca cgccagtata gagacgaatc tgcggcttgc
      301 ccgccctgat gccacccggg aagagatcct ggaggcggcc cgactggccg gcgctcacga
      361 attcatcacc caactgccgg aaggctacga cacggtggtc ggcgagcggg gagagcgact
      421 gagcggtggt caggcgcagc gcatcgcgct ggcaagggca tttctgaaag acgcccctct
      481 gctgatcctc gacgaggcgg ccgctcaact ggacgtcgag aacgaggcgc tgctcgagga
      541 atcgctggat tgcatcatgg agggacggac ggtgttgctg atcgcccaca ggctgggtac
      601 ggcgcggcga gccgaccgcg tcgtggtgct cgcggagggg cgcgtggcag agtgtggccc
      661 tcacgaagag ttgaggcgac ggggcgggct gtacagcaga atggtccagg cggccggcga
      721 gggggacgcg gagacgggga gacacagaga cacggggacg cggggacacg gggacacggg
      781 gggccctcac ccgcccttcg ggcggcctct cccagaggga gagggtcggg gcgagggcac
      841 tcagcactca gcacccagca cccaacactc atcactccac acccagaact cagaacccag
      901 aactcagaac ccagaaccca gaaccctcgc agggctgctc ggcctcctcc gccccctctg
      961 gggctggatg gcgctctctg ctctcctcgg gttctgcacg gtcgccgcag gggtgggctt
     1021 gatggccaca gcggcatttc tgatcgcctc cgccgccctc cacccatcgg tggcagacct
     1081 ggcggtgcct atcgtcggcg ttcgattctt cggcctatcc cgaggagtct tccgctatct
     1141 ggagcggtac tgctcccact acgccacctt cacgctgctg gcaagattga gggtgagctg
     1201 ttatcgggcg ctggagccac tggctccggc ggggctaatg gggcaacggg gtggagacct
     1261 gctgtccagg gtggtggcgg acgtggagac gctgcaaaac ctctatctca gggtcgtcgc
     1321 cccaccagtc acggcgctgc tggtcgtcgc ggccatggtg atcttcctgg gggccttcgc
     1381 accagtgctg atcctgccgc tgatcgctac agcactggcg gcgggcctgg gagtaccgct
     1441 gctatcgggc ctggcatcca gaggactggg ggccggctcg accctgctac ggtcggagtt
     1501 gaacgtccag ctactggatg ggctccaggg gatggcagac ctggtggtct tcggtcgaga
     1561 ccgccaccaa ctgaagctcg tgacacacac taaccggcag cttgcagccg tgcaacggca
     1621 ggccacggtc gtcagcggac tccaaagcgg gcttctatcg ctgcttgatg ggctcggcat
     1681 gtggggagtg cttgcgatag ccctctcact cgtcgccgcg ggccggctgg acggggtcta
     1741 tctgccggta gtggggctgg cgaccctcgc ctgtttcgag gtgctggctc cccttcccct
     1801 ggcagcccag cagatcgagg gtagccttgc cgccggacgc cgccttctag aaatcgcgtc
     1861 tgcggagaag atggagcccc aggccaccgc caattgggat caccattccc tcacgccctc
     1921 gctgtcgata cgtgatctca ctttcagcta ccgccagggc cagccccccg ccctggatgg
     1981 ggtgaaccta caactggagc cgggccggtc cctggccatc gtcgggcgca gcggcgcggg
     2041 caaatctacc cttctccatc ttctgctgcg cttctgggac tacgaagagg gcagcgtgct
     2101 gctcggaggc agggaactgc gggaaatccc gccggaggag tcacgccggc tgatggcggt
     2161 tgtcccgcaa cggacccacc tcttcaacct cactatcagg gaaaacctga tgcttgccag
     2221 gccgaacgcg accgagcaac agatggtcga ggccgctcgg caggcccggc tccacgactt
     2281 cgtcgcgtcg ctcccactgg gctacgacac gtttgtagga gagcagggca tccgactcag
     2341 tgcaggggag cgacgtcgca tcgcgatagc ccgggctctc ttgaaggacg cgcctatcct
     2401 gctgctggac gagcccacag cgggcctgga cagcctgacc gagaaggaag tcatggacac
     2461 cctgagggga gtcatggcag gtcgcaccac gctgttggtc acccacaggc tggttgggct
     2521 gcaagaaatg ggagagatct tgctgttcca gaacggccgc gtagttgaaa ggggaaccca
     2581 gcagcagttg ctgagccaag acggtcacta ccgccgcagc tgggaactcc aacgacaact
     2641 cctgccccag gagtgagcga cctctgtctt ggagtgcagc gacttgacac cgcaacgttg
     2701 ccaagcacat cgcgccgaag cggccaagcc agcgcaatgc cgtagggcgg ggtcttgtgc
     2761 cccgcctccc acaccgcgag tacgaccacc aagccgcctt ccggccacgc tctaggcggg
     2821 gcacaagacc ccgccctaca tgacgtcgga ggcagtctcc acaagcatgg cgaccaccag
     2881 caaggccatt cactagctcg tgttggatct tggcccgaac gttcctggcg gttccggcta
     2941 aagcctctac tccacttggt gtcgccaccc cacgcgggca cgggcaagcg gtgaacacca
     3001 ttttgtatct cacagataac agccgccacc ccacgcgggc acgggcaagc ggctcccagc
     3061 caatttgatg tctccccgtt cgcggcatgc ttcttgcttt tgaagatgca gtcgtttctc
     3121 cgcgtctgta taggttacct ctggttctcc caactactgg ctgggaaatg gggcacgagg
     3181 ttgtgcagtg gattatgtcc gcctgacaaa acccgggagc gtcgggctgc tacttctgac
     3241 cgctcttgcc ggcatgaccg cggtcggttc cggcttgcca tccctctcgt tggtcgtggc
     3301 gaccctggca gggggagcgc tggcggcagg cgggtccaac gccctcaact gctatctgga
     3361 ccgcgacctg gaccgactga tgccgcgcac agccaaccgt cccctccctt ccgggcggct
     3421 ggctcccctt aaggccgccg ccttcgggat actgctctgc cttctgtcct ttgtcctgtt
     3481 ggccgccacg gttaacctgc ttgccgccct cctggccatg gcgggaatga tctactacgt
     3541 ggtcgtctac accctctggc tgaaacgtcg gtcaccctgg aatgtggtgg taggcggggg
     3601 agcagggggc ctccctctgc tggtgggctc cgccgcggca acaggagaga tctccccgtg
     3661 ggcgctgatc ctgggagctg tgatcttcct atggacgccg ccccacttct ggtcgctggc
     3721 gctgctccgc cgagacgagt atggccgagc cggacttccc atgatgccgg ttgtccgcgg
     3781 cagtctggaa accaggcggc aaatactgct gtacagcatg ttcctcgtcg gactctccct
     3841 ccttgttccg gtcatgggtt tcaccgggag cgggccccta ttcgtggccg ccatccttgg
     3901 aacagtgatg ctgatgctgg cgctgcgttt gctgaggcac gagtccccgc gggcagcgcg
     3961 acagctatat cgatactccg tcctgtacct agcactcttg ctcagtacgt tgatccttga
     4021 tcgcgccggg ggcttgcccg ctctgttggg cacttcgtag cccgaccggc gtgggaggta
     4081 gagaccatgc gaccgggtag ccacgcccat ctcaaggcgg tcccgttaac ggccatggtg
     4141 gtcctgggag cggcggtgct cgcaggctgc cagcaggctc cggctgccat cttcaggccg
     4201 gaatcgccgg gcgccaccgc catccacaat ctatcccttt acctcctggc cgtggccggc
     4261 gtcgccctgg taggggtcga ggtgaccctc ttcctcgccg tcgctcggtt cagcaaccgg
     4321 ccggaggaag aagccgttca aacccacggt aacttgaagc tggagaccgg ctggacggca
     4381 gcgacggcca tcgtcgtctt cataacgctg gggctgacgg tgaatacgat ggttgatgtg
     4441 accgcaaaca tcgccgagtc ccctccgtcc ctggccagcg cgatgccgct gcaatccgcc
     4501 tggccgggcg ataccctgcc gatgagggtg gtggggcatc aatggtggtg gaccttcgaa
     4561 tacccccagc aggggatcgt cacggcgaac gaggcccacg tacccgtggg ggacaccgtt
     4621 cgggctcaac tggagtccgg cgacgtgatc cacagtttct gggcgcccag gctgggtgga
     4681 aagaccgaca tgatcccggg ccagatcaac tacacctcct tcctggccgc cacaatcgga
     4741 agctacaccg gagagtgcgc cgagttctgc ggcgtggagc acgcccacat ggggttcaca
     4801 atagtcgtcg actcgccggc ggacttctcc gactgggttg cgggacagca ggcaccggct
     4861 gcgagcgccg gcaccgacgc agagcgagca ggctaccaga ccttcacacg aagctgcggg
     4921 gtctgccaca ccatcaatgg gacggaggct cgcggcaaat ccggcccaaa cctgacccat
     4981 ctggccagca gacagatgct ggcggccaat acgctggaaa acaacccgac caacctggcc
     5041 aggtggatcc aggaccctca ggggctaaaa ccgggcaacc tgatgccgaa ccagaagctt
     5101 gaccagcagc agataagcga cctggtcgcg tacctgagca tcttgaagta gccagcgcgt
     5161 acagggggag ttcgcaatgt cgagtacagc agacagcttg cgagggcgca actgggacga
     5221 tagcggcata tggagctgga tcaccaccac gaaccataag cggatcggcg tgctatatct
     5281 gacgaccagt ttcgtgttct tcctggccgc cgccgccatg gcgatgctga tgcggaccca
     5341 actcatcaag ccggacaacg acttcctgtc tcccgatgtc tacaaccaga tcttcactat
     5401 gcacggcacg accatggtgt tccttttcgg catgcccgtg ctggcggggt tcggcaacta
     5461 catcgtcccg ctgatgatcg gcgccaggga catggtgttc cccaggctga acgcattctc
     5521 ctactggacc tacctggccg gagcaatcct gttgtacagc agcttcgtct tcggaggggc
     5581 gcccaacgcg ggatggttca gctatgcgcc gctcaccgag atgacctact cccagacggc
     5641 cggcatggat tactggagcc tctccatcat catgctgggg atgtcctcca tagccggcgc
     5701 ggtcaacatc ttcgtcacga tcctcaccct gagggccccg ggcatggggt tcaaccgcat
     5761 gcccctcttc gtgtgggcaa ccttcgtcaa tcagttcatc atcctggtgg cactgcctag
     5821 cctgaccgcc gcggccatca tgctctatct cgatcgccac tacggaacca gcttcttcaa
     5881 tccggccacg ggcggggatc cattgttgtg gcagcacctc ttctggttct tcgggcatcc
     5941 ggaggtgtac atactgatcc tgcccgcctt cggcatcatc tccgaggtga tcccggtctt
     6001 cgcgagaaag ccgatcttcg gctactcctt catcgcctac tcctccgtgg ccatcgggat
     6061 actcagcttc gcagtgtggg cccaccacat gttcgccgtg ggcatgagcc tggcggccga
     6121 tgccgtcttc gccttcacca gcttcaccat cgccgtgccg accgcggtca agatcttcaa
     6181 ctggctggcg accctctggg gtggcgcctt gcgactcaag accccactgc tcttcgcaat
     6241 gggcttcatc ggtctcttcg tcattggagg cctcagcgga atggccgtgg cggttgtgcc
     6301 cttcgactgg caggtgaccg acagctactt cgtggtggga cacctccact ccgtgctgtt
     6361 cggcggcacg gcattcggcg tctttgccgg catctattac tggttcccca agatcaccgg
     6421 caggatgatg gacgaacggc tcggggtact gagcttcgcg ctcctcgtcg tcgggttcct
     6481 cttcaccttc caacccttcc acatcctcgg gctgctggga atgccacgga ggatctacac
     6541 cttcgcgggg aacatggggt ggaacgaact gaacctgatc ggaaccctgg gagcctacgt
     6601 gatcgccgtg gcagcggtgg tcttcctatg gaacctgatg gcgagcctgc gcaatggccg
     6661 cgtcgccggt cccgacccgt gggacgcctg gaccctcgaa tgggccacgt cgtcaccgcc
     6721 gccggtccac aacttcgacc gaatgcccga ggtgcgcagt cggcggccgt tgtgggacga
     6781 gaagcatcct gagcgagccg actatctggt gaggcactaa ctggcaatca gttgtcagcc
     6841 atcagcagtc ggcgcaagac aggatcgaag aggagtggca acgcagagaa gaacaacggt
     6901 tgttgggtat ccagtccaat ggacacttgg agctgacagc tgatcgcgga gggttaactt
     6961 ggtgggtgac atagagactg agggccccgc cggaaccgaa cctggcgctg agcccaacgg
     7021 gcataaagtc ggcgagcatc ccgccgtctc ggagcacagc ccctggcctg tcctgctgtc
     7081 gctggggatc ctgatcgtgg caatagggtt gctgtaccag ccggccatcg tggcggtggg
     7141 tgcagccgtc tgcgtagccg cagttattgg gtggctatgg cagccatggg tctcatgagg
     7201 cagggagggc ggcgggatga ttgctagaac catgggccac ggcccgcagc cactggcgac
     7261 ccgattcgac cccacgatag tggggctgtg ggtcttcata gctaccgagg cgctcttctt
     7321 cggcatgctg atggggtcgt acctctacct gcgcgttcgg ctggggagtt ggcccccacc
     7381 cgggactccc gagagggatc tcttcgtgcc gctgctgaac acggtggtgc tgctctccag
     7441 tggattggtc atgcacggag cccacgacag catccgccgg ggcgaccgca gcgtgcttca
     7501 gggagggatc gtggccacga tgttcctggg ggcggcgttc ctgggcggcc aggcatatga
     7561 gtatacccac gccgggttcg gcctcagcag tggactgttg ggctctacct tctttaccct
     7621 gaccggcttc cacggtgccc acgtctttgc cggcctggtc ttcctggcgt tgatcctgtg
     7681 gcgtgcgagc aaggggtact acacggccaa ccatcacgtc ggagtcgagg cgagcgcgct
     7741 ctactggcac ttcgtggatg ccgtttgggt agtgctgctg acgattcttt acctcatata
     7801 gcggctgaac gactcacagt ctctggaggg aggcaagata cgacccgatt tcgggtgcag
     7861 gcagactcac aagaggcact cagtgcccgc cacagtgtag ataagactgt catgttgagc
     7921 gaggtgaagc atctcccggc gtgaacggag agatccttcg ctgtcgctca ggatgacatc
     7981 gggcaggatc actgtggcag agatttggga gggatacttt ggagaagaaa ctggcgctac
     8041 tgactgcggc gatcctcgtg cccaccatta tcatggcctc cgcctgcggc ggtggggcgg
     8101 cccccgcgcc cacttccgca ccggctgcgc cggccgccac caaagcaccc gcagcgactt
     8161 cggccccagc ggccaccaag ctggcagcca ccccagcagg tgcagccact ccagcgggtg
     8221 cagctaccgt cgcggcaggc ggatcgacta cggccccggg tgcaaccacc actacaccgg
     8281 cagcatcggc gtcggccgcg ggagatgtca ccgcgggcaa ggcaatcttc gaccagaact
     8341 gcaaccagtg ccaccccaac ggggacaagg gtgccggccc ggcactgcgc ggaagaaatc
     8401 tgtccgccga ccgaatcaag aaccaggttc ggaacggcgg gggcggcatg cccgctttcc
     8461 cggccagcag gatcacggat cagcaactga acgacctggt ggcctacgtc caatcgctgc
     8521 aatagccgac gggggcagag gatgagatct tccatgccta gaatcggatg ggctgtctgg
     8581 tcgctcacgc taatgttggc ggccgcagct ctgacaggct gccagtccgg ccgggcttct
     8641 ggggcggctg cgtcggtggc cccgagcgcc gctaccccgg cgtccaagac ggtctggaac
     8701 ggccactatg cccaagatat ccagccgatc ttcaacgacc attgcgtaac ctgccacggt
     8761 cccaacaggg cagacaatgg cctgaggctg gacagctatg agggggtcat gaagggcacc
     8821 aaccacgggc ccgtggtcgt agccggcgac cccgaccaca gcgcgcttat atctgtggtc
     8881 cagggtagcg tggagccaag cataaggatg ccccacggtg ggcagaaact cacagagcag
     8941 gatgtgcaga accttcgact ctggatcgca gccggagcca gttctaatta gagggtgaat
     9001 accttacgtc ccctggtagg agtggatcgc catgcaaagc gagactgctg agaaacagca
     9061 gatcgaaagg cgcacctttt tgggctacgt cattgtggcc gtcggggcct tcatcacagc
     9121 agtgctgggc atcccgttga tcggatcgct gatagcgccg gctctacggg gccgcgagac
     9181 caactgggtc accgtggggt ccactatgga cttctccgtc ggccagccga aggcagccac
     9241 ggtcaccgtc accaccaagg acggctggat tacccagcag gagtccaagg gggtgtgggt
     9301 tatcaaacag agtgagacca gtttcgtggt gtacaacggg cgatgtaccc acctgggctg
     9361 cgcctacaat tggagcccgc cccagcagga gttcgactgc ccgtgccacg gcggaagata
     9421 ctccctggac ggcaaagtgc tgggaggacc tccgcctcgc cccctggata cccttcaatg
     9481 gagggtcgac cagggaaacc tggtgatccg gtacgaggat ttccggctgg gtgtccctca
     9541 gaaggaggtc gcatgacaac gactgcacca tcgccggagc cgaagcccga gggacgggtg
     9601 gggcaggtga gcggctggtt gttggaacgg gcgggcgcca tggggatctg ggaatctttc
     9661 tttgcccgca aggtgccgct gggggtggga tggctgtata ccctcggctt cgccagcatg
     9721 ttcgccttca ccgtccaggc cgccactggg atcttcctgg ccgtttacta ctcgcccagc
     9781 ccggaccacg cctacgatag cattcagttc atcatgtttc aggtgccatt cggcgccgta
     9841 gtgagagggt ttcaccactg gatggccagc gtcatggtgg tgctggtgat cctgcatatg
     9901 gcgacggtgt tcgtcctggg cgcctacaag taccccaggg agctcacctg gatggtaggc
     9961 gtggggctgt tggtggttac cctggggttc gccttcaccg gctacctgct gccgtgggat
    10021 cagaaagcct actgggcgac cgtcgtggga accaacatcc cgggcaccct tcctgttgtc
    10081 ggagacctga tcctgaggat catgcgcgga ggggcgcagt tgggcaccct cacgctgacc
    10141 aggttctact cgttccacgt tctgctgctg ccggcctcac tggcggcact gttgggcgcg
    10201 cacctctacc tcatcgtaca ccacggggtc agcgtgccgc cctggctgtg ggacagcgtc
    10261 gcgccgaggg tgcgcgccgt gttggggcat cgagaggaag ccgagagagc cgctacccgt
    10321 gaagaggcgt accaccgacg ctacgagcag ttcaagcggg agggacaccc cttctggccg
    10381 gacgtcatcg tgcaggacac gctggcggcg gccgtggtgc tgctatgcac gatcggattg
    10441 atggtaacca tgggggtgcc gacggaggag ccggccgatc ccaccaatac ggcctacgtc
    10501 ccccgcccgg agtggtactt catgttcctc ttccaggccc tgaagtactt ccccgggaac
    10561 ctggagtggg tcggcgcggc ggtgctgcct ggggtggtga tgctggcaat actgctgctg
    10621 ccggtctacg accgaggccc aaaccgggcc ccatggaagc gcccattggc catgacggcg
    10681 ggcgcagcga tggcagccat agtcggctac ctgacctatg tggcctacca gtagacaatt
    10741 tggacgtagg gcggggtctt gtgccccgcc gaccgcaccg tggacacgac caccatggtg
    10801 tttccggccg cgcatttggc ggggcacaag accccgccct acgagacgtc ataggtggcc
    10861 tgcgaaggct aagcggtcac aagcaaggct caactaaacg gtcttggatt gatccccgat
    10921 tccacaggag tagacacgat gaaatcggtt cacctcatag ggctggggct tgtcgtgggt
    10981 agcctggccc taggcaccat gatatcggta atgactttcc ggtggcaggt ggaaacgttt
    11041 ccctccaccg gcccggtagt tgcagccgcg gccgggcccg ccggcgccgc catgcctgca
    11101 cactcctcca ccgacgccgc gaaggcgaag gccctcttcg acagcaagtg cagtagctgc
    11161 cactccatag ggggtggcaa gaagatgggc cccgacctga agggggtggc aacccggcga
    11221 tcggccgact ggatcgcgca gttcatcatg gccccggacc aggtgatcgc cgctggcgat
    11281 caggcggcca ctcagatggt gaaggagtac ggaatgccga tgcccaatct cggcatctcc
    11341 aaggaggacg ctgccacgct cacggcctac atcagccagt aataccgtca aggtcgccgg
    11401 ccggttccgg cgacctactc ctctcatccc tgaagtgtga tcctcggctt ctccctttcg
    11461 gccctgccgg gtggcaggca gctttccccc tactgccatc actgtcgaac gagtcatcac
    11521 tttccgcgtt gacaactctc aagatcccgc ctatacttcg cgcagacagg atagttcgtg
    11581 ttgaacgaac ttttcgcatt acagcgatta tccgcactgc ggtaacaggt tggagggtgc
    11641 cgatggctta tgaccgggac gagactgtca agcacatcct tgccatagag aagcacatct
    11701 accagtgggt ccagttaggc ggtattccca gctgggccga gaccgatctc acgatgcccc
    11761 agctgaaggt gctcatcctc gtggacaggc accaccctat gcccaccagc caggtcgccc
    11821 gcacccttgg gatgaccctc tccaccgcca ccggggtagt cgatcgcctg gtaagccagg
    11881 agttgatcga gcgactggaa gaccctgccg atagaagggt tgtgc
//
LOCUS       DATALS010000322         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_168669, whole genome shotgun sequence.
ACCESSION   DATALS010000322 DATALS010000000
VERSION     DATALS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168669"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /gene="malQ"
                     /locus_tag="VHS06_07195"
     CDS             complement(<1..1147)
                     /gene="malQ"
                     /locus_tag="VHS06_07195"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX2987941.1"
                     /translation="MSEGLKRGYLRRESGLLLHPTSRPSAHGIGDLGVEARQFVDFLD
                     QAGQRLWQILPLGPLGSGNSPYASPSAMAGNPLLISLSLLADEGLLPSRRLLDVPAMP
                     AKQVDYSAVAGFKIPLLRDACRHFLADASSLQRSDYQSYLDSNRFWLEDYALFAALKE
                     AHGGRSWIEWEPEIASRRPSAMDHWYRQLSEEIAFHQFSQYLFFRQWDALKQYANLHK
                     VRIVGDIPIFVALDSSDVWARPELFHLDSSGRPTVVSGVPPDYFSKTGQRWGNPLYRW
                     DVMAREGYNWWVERFRVALSQLDLVRIDHFRGFQAYWEVPANRKTAEYGRWVPGPGTA
                     LFRALEEALGPLPVIAEDLGVITPDVEALRLRLSNPPAWRAWQARRAA"
     gene            complement(1164..1625)
                     /locus_tag="VHS06_07200"
     CDS             complement(1164..1625)
                     /locus_tag="VHS06_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2987942.1"
                     /translation="MYKHLLVPLDGSKLAEDALPHAEAIARRFQSKITLFQVAPVVPI
                     PPSVDPMTGTGNEAIVALEASETAELEARQYLKEVASRPILESIAVTQEVTRGKAARE
                     IVQRANKRDIDLIVMSTHGRSGLGRLVFGSVAEAVLRESGTPILLIHSREK"
     gene            complement(1850..>2719)
                     /locus_tag="VHS06_07205"
     CDS             complement(1850..>2719)
                     /locus_tag="VHS06_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2987943.1"
                     /translation="IESIFSGTDMMATSVGITARVLRDLGQLDSSESRVILGAAVIDD
                     ILAMVALAVVSGLATTGSVRLSEVVLVVAEALLFTLFVALGGTRTVRRYGHQLTRLRM
                     EGAPLAISLLVMLGLASLSAQIGLAAIIGAFLAGMAFAEAPDEFDLERQVLPIYQFLV
                     PYFFVLTGSQVDWRLFTNGGLMALALGITFLAILGKLLGCGLPMIPFGVRSAAIVGVG
                     MVPRGEVGLIVASLGLTMGVVAVEIFSVVVIMSILTTIVAPPVLAALFAGRGMPRPEG
                     EEEGEERAQLPDL"
BASE COUNT          517 a          890 c          808 g          504 t
ORIGIN      
        1 aggctgctcg acgtgcctgc catgcccgcc aagcaggtgg attactcagc cgtagcctca
       61 gagcctccac atccggggtg atcacgccca aatcctccgc tatcaccggc agggggccaa
      121 gcgcctcttc cagagcccgg aacagtgctg tcccaggccc tgggacccac cgcccgtact
      181 cggcggtctt acgattcgcc ggcacctccc agtaggcctg aaagccccgg aagtggtcga
      241 tcctcaccaa gtccagttgt gacagcgcca ccctgaaacg ctccacccac cagttgtagc
      301 cctccctggc catcacatcc cagcggtaga gagggttccc ccatctttgt ccggtcttgg
      361 agaagtagtc ggggggcacg ccgctaacca cggtgggtct cccactgctg tccaggtgga
      421 agagctcagg ccttgcccag acgtcggagc tatccaatgc cacgaagatt gggatgtcgc
      481 cgacgatccg gaccttgtga aggttggcgt actgcttgag tgcgtcccac tggcggaaga
      541 acaggtactg gctgaactgg tggaaggcga tctcctcgga gagttgcctg taccagtgat
      601 ccatggcgga cggccggcgc gaggcgatct ccggctccca ctcgatccag gaccttcccc
      661 cgtgggcctc cttgagcgcc gcaaagaggg cgtaatcctc cagccagaag cggtttgagt
      721 cgaggtaaga ctggtagtcg gaccgttgga gggacgacgc gtccgccaga aagtgccggc
      781 aagcatccct caacaggggg atcttgaagc cggcaacggc cgagtaatcc acctgcttgg
      841 cgggcatggc aggcacgtcg agcagcctgc gagaagggag aagcccttca tcggccagaa
      901 ggctgaggga gatcagcagc ggattgcccg ccatcgccga gggggatgcg tagggggagt
      961 tgccgctccc caacggcccc agcgggagga tctgccacaa ccgttgccct gcctggtcca
     1021 ggaaatccac gaattgcctt gcctccaccc ccaggtcccc gatgccgtgc gcgctgggac
     1081 gggatgtggg gtgcagcagc aggccgcttt ctctccgcag gtagcccctc ttcaggccct
     1141 cgctcatgtc ttcctctggt tcgctatttc tcgcgggagt ggatcagcag gatgggcgtc
     1201 ccggattccc tgagcacagc ctcggctacg ctgccaaaga ccagccggcc aagccccgat
     1261 ctgccgtgag tggacatgac tattagatcg atgtcccgct tgttcgccct ttgcacgatc
     1321 tccctggcag ctttgccccg ggtcacctcc tgggtgacgg cgatgctctc cagaatggga
     1381 cgcgaggcga cctccttgag gtattgtctc gcctccagtt ctgccgtctc cgatgcctcg
     1441 agagcgacta tcgcctcgtt ccctgtgcct gtcattgggt cgacggaagg gggtataggg
     1501 accacaggcg ctacctggaa cagggttatc ttcgactgaa aacggcgcgc gatcgcctct
     1561 gcgtggggca gcgcgtcttc tgccagcttt gacccatcca atggcaccaa gaggtgttta
     1621 tacatgatgg gcacctcctc tctgcaactg taccattttg agacgatccg tgccgtatag
     1681 caacagagga ggagagtccc atcgccatca ggctgacggc gatggggtcc ttcggcagtt
     1741 gtcgctcttc ctgccgccgc gctcctgacg ttgtacgtgt gcctttattg atcgatacct
     1801 acttgggagc aacgcgtcag agaagcggtc tcggactaaa gcatgacgct cacagatctg
     1861 gtagttgggc tcgctcttcg ccctcttctt ctccctctgg ccggggcatg cctcgtccgg
     1921 caaacagggc ggccaggacc ggtggcgcga cgatcgtggt caggatgctc atgatgacga
     1981 ccacgctgaa gatctcgacg gccaccactc ccatggtgag gccaaggctg gccacgatca
     2041 aacctacctc ccctctcggt accataccca cgccaactat cgccgcgctc cggacgccga
     2101 aggggatcat cggcaggccg cagccaagga gcttgcccag gatcgccagg aaggtgatcc
     2161 ccagcgccag cgccatcagc cccccgtttg tgaacagtct ccaatccacc tgacttccgg
     2221 tgagcacgaa gaagtagggc acaagaaact ggtatatggg aagcacctgc ctctcaaggt
     2281 cgaactcatc cggtgcctcg gcgaacgcca tgccggccag gaaggcccca atgatggcag
     2341 ccagcccgat ctgtgccgag agggacgcca atcccagcat caccagcagg cttatcgcga
     2401 gtggggcccc ctccatcctc agcctcgtca gttggtggcc gtatcgccgg actgttctcg
     2461 ttccccccaa tgcgacgaag agggtaaaga gcaacgcttc cgcgacgact agcaccacct
     2521 cagagagtct gacactacca gtggtggcga gcccggacac caccgccagc gcgaccatgg
     2581 ccaggatgtc gtcgatcacg gcggccccca ggattacacg gctctcgctg ctgtccagtt
     2641 ggcccaggtc gcgcaggacc cgggcggtga tacccacgct ggttgccatc atgtcggtcc
     2701 ccgaaaagat cgactcgat
//
LOCUS       DATALS010000323         8094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_218380, whole genome shotgun sequence.
ACCESSION   DATALS010000323 DATALS010000000
VERSION     DATALS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218380"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VHS06_07210"
     CDS             <1..573
                     /locus_tag="VHS06_07210"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX2987944.1"
                     /translation="DRIISNASCTTNCLVPVAKVLQDTLGITRGLMTTIHSYTNDQRI
                     LDQVHKDLRRARAAAQNMIPTSTGAAKAAALVIPELKGKFHGISIRVPTATVSIIDLV
                     CDVERETSAEEINDAFRKASEGRMKGILEITMDPVVSSDFRGYPASSIVDGQSTMVMG
                     GTMVKVLSWYDNEWGYSCRVGDLAAYIASR"
     gene            578..1763
                     /locus_tag="VHS06_07215"
                     /pseudo
     CDS             578..1763
                     /locus_tag="VHS06_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018670226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
     gene            1848..3398
                     /gene="gpmI"
                     /locus_tag="VHS06_07220"
     CDS             1848..3398
                     /gene="gpmI"
                     /locus_tag="VHS06_07220"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX2987945.1"
                     /translation="MARIEPVVLIIMDGWGIAPEGPGNAVTLAKTPVVDALWEKYPHT
                     QLEASGTRVGLPAGQMGNSEVGHLNIGAGRVVYQDLVRISLAVQDGTFFENPALLRAI
                     NHVREHDSKLHLLGLLGPGGVHSHIDHLYALLSLVAKHHVKKVEVHPILDGRDTPPQS
                     GLGYIKELEQRIATLGTGRIATVAGRYYTMDRDKRWDRVEKAYRCMVFGEGNSGPSAE
                     AIVEKSYADGVTDEFVIPSVVRETPEKGEESLIRPHDALIFFNFRTDRTREITRALVM
                     DDFPFFNRGPKIEGLDVITMTEYEEGLPVTVAYPSEFLNEVLADVLAEHDICQFHTAE
                     TEKYAHVTFFLNGGREEPFPEEDRKLIPSPKVATYDLQPEMSAHEVTQVVLDVLKQGH
                     HQFIIINYANGDMVGHTGSIPAAIKAVETVDEGVGKIVDAVLAHAGAALVTADHGNAE
                     EMIDPATGGPWTAHTTNPVPFILVCERTKGMRLRDGGALSDIAPTIFDLMGIPKPDLM
                     TGRSLIVK"
     gene            3420..4184
                     /gene="tpiA"
                     /locus_tag="VHS06_07225"
     CDS             3420..4184
                     /gene="tpiA"
                     /locus_tag="VHS06_07225"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX2987946.1"
                     /translation="MSRIPIIAGNWKMNTNLEEAVALVEAMRPRLEAISGVEVVVCPP
                     FISLAAVQGHLHGSPIGLGAQDMYFAEKGAYTGEISPLMLRGLVDYVIIGHSERRQYF
                     GETDELVAKKVKAALDRGLKPIMCVGERLEEYEAGKTQEVVTRQVRGGLSGIESLGDL
                     VVAYEPVWAIGTGKAATGQGANEVIGMIRRLFGSKFGSEAASALRIQYGGSVTPDNIR
                     EFMEQPEIDGGLVGGASLKADAFVEIVRQTAEAKRA"
     gene            4360..4857
                     /locus_tag="VHS06_07230"
     CDS             4360..4857
                     /locus_tag="VHS06_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEX2987947.1"
                     /translation="MEALEAAVPQATVEAVIADLGVAEQRRRKLPAELTLLLSVAMNL
                     FTHHPLEEVLRKLLRGLRLIWPEDDFATAGKGGISKARYRLGAAPLVELFHRVCQPMA
                     TEDTPGAFLFGLRLMALDGTNEDLPDTVENARVFGRPGGNRGDGAFPQAKGFYLSECK
                     SGSWS"
     gene            complement(4832..5383)
                     /locus_tag="VHS06_07235"
                     /pseudo
     CDS             complement(4832..5383)
                     /locus_tag="VHS06_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            complement(5567..6856)
                     /gene="eno"
                     /locus_tag="VHS06_07240"
     CDS             complement(5567..6856)
                     /gene="eno"
                     /locus_tag="VHS06_07240"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX2987948.1"
                     /translation="MTVIEDVFARQILDSRGNPTVEVEVILEDGTVGRAEVPSGASTG
                     AHEALELRDKDATQFGGAGVRKAIENITDLIAEELQEMDVLDQVGIDRMMIEMDGTPN
                     KSKLGANAMLGVSLACAHAAANSLDLPLYRYIGGANAHMLPVPMLNILNGGKHAENST
                     DLQEFMVMPVGARDFASALRMSAEIYQALKKVLKGKGLNTNVGDEGGFAPSLRSNQEA
                     VDVILEAIGAAGYKPGEEVFLALDPAASELYENGKYNLKREGRSLTSEQMVQFYSDWV
                     RQYPIISIEDGLAEDDWEGWAMLTRELGDRVQIVGDDLFVTNTARLKRGIEEKSANSI
                     LIKLNQIGTLTETLEAIQMAQRAGFTAVVSHRSGETEDTTIADLSVATNAGQIKTGAP
                     ARGERVAKYNQLLRIEDQLGENSLYAGREAFYNIRRQ"
     gene            complement(6978..8051)
                     /locus_tag="VHS06_07245"
     CDS             complement(6978..8051)
                     /locus_tag="VHS06_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransacetylase family protein"
                     /protein_id="HEX2987949.1"
                     /translation="MATIYVMSMESFAGKTVFCLGLARKLADDGFSVGYIRPLTEAAR
                     NIESANTCAAIMKRSLGLMESMDILDPVLVDGETLDWILSDRAVDYSQKVMDAFKISS
                     SGKDVMVVEGVSGLSEGYMVGLDAKTTVNMLGAQCVLLVRPKDEMIADSILSAKSWMR
                     DRMTGVIFNAVPRGMVEFVAESLTPYLNRRGIKVYAIIPEDRMLMAVTVQDLVDRIGG
                     RVISGSDHMDRLVETVMVGAMSGENALSYFRRKQHKVVITGGDRPDIQLAALETSTGC
                     LMLTGNLNPSPIILGKAEEMGVPVVVASQDTLTAATLVEQAFTEVRFHEEKKIKRYER
                     LLESHLNYEELRKDIGLAIKSSD"
BASE COUNT         1543 a         2506 c         2490 g         1555 t
ORIGIN      
        1 gacaggataa tctccaacgc gtcctgcacg accaactgcc tcgtgcccgt ggccaaggtg
       61 ctccaggaca ctctgggaat cacccgcggc ctcatgacca cgatccactc ttacactaac
      121 gaccagagaa tcctggatca ggtccacaag gacctccgca gggcgagggc ggcagcccag
      181 aacatgattc ctacctccac cggagccgcg aaagccgcag ccctcgtcat cccggagtta
      241 aagggcaagt tccacggcat ctccatccgc gtcccaacgg caaccgttag catcatcgac
      301 ctcgtttgcg acgtggagcg ggagacgagc gcggaggaga tcaatgacgc cttccggaag
      361 gcctcagagg gacggatgaa gggaatactg gagatcacca tggacccagt agtttcctcc
      421 gacttccgcg gatacccagc atcctccatt gtggacggcc agtctaccat ggtcatgggc
      481 ggcacgatgg taaaggtgct ctcctggtac gataacgagt ggggttattc ctgccgggtg
      541 ggcgatctgg cggcctacat cgcgagcagg taggacaatg gaaaagcaga ctatccgcga
      601 cgtggacgtc acggggaaga ccgtcatagt cagggtggac ttcaacgtac cccaggacaa
      661 ggaggggaac gtgacggata ccacccgcat cgaggcggct ctccccacca tccgctacct
      721 gttggagcgg aacgccaagg tgatcctgat gtcccaccta ggccgtccag acggcaaggt
      781 cgtggagaag cttcgcatgg cacctgtggc gaaggaactg gagcgcttgc tcggccagcc
      841 ggtccaggtt gcgccggatt ctgtcggtcc cgaggtcgag caggtggttc gcgacctgga
      901 acccggccag gtgttgctct tggagaacct ccgcttccac ccggaggagg aggccaatga
      961 tgcgggtttc gcaaagcagc tcgcatccct ggccgacctg tatgtgaacg acgccttcgg
     1021 gacggcccac cgcgctcacg cgtccacggc gggcattgcc gactacctcc cggccgtcgc
     1081 cggcttcctg atggagaaag agctaaacgc gctcggcggc attctcgaga acccccagag
     1141 accgctggcc gcgatcatcg gcggcgccaa ggtttcgacc aagatcaagg tgcttcaaaa
     1201 cctgctttcc aaggtagata ccctgatcat tggcgggggg catggcaaac accttcctta
     1261 aggcgaaggg tttgaacgtc ggcaagtccc tcctggaaga gggaatgctg gacgtggcgc
     1321 gggacttgat gaagcatgcc gagaagcgag gcgtgaagct cggcctccca gtagatgtgg
     1381 tcgtggccga gcggttcgaa gccgaggcac ccagcaagat cgtagacgtg aaccaggttc
     1441 cggagggctg ggtcatcatg gacattggcc cgaggactat cgacgagttc gccggtctgc
     1501 tgaggccagc taagaccgca ttctggaacg gccccatggg cgtcttcgag atgccacgct
     1561 ttgctggggg cactaaagcg attgccgaca tcctggcgaa atccgatgtg atctcggtgg
     1621 tgggcggcgg cgactcggtg gcagccgtgg agcagatggg ctacgccgat cggatgactc
     1681 acatctcgac cggcggtggt gcctcgttgg aactagtgga gggccgaacc cttccagggg
     1741 tggcggcgct gaaagacaaa tagctgcgct gagtgctgag ttctgggttc tgagtttatc
     1801 gcctcccact cagcactcag aactcagcac tcagaactgg gaacgatatg gcacgtatag
     1861 aacctgttgt attgatcatt atggacggct gggggatcgc acccgagggt ccgggcaatg
     1921 ccgtcacttt agcgaagacg ccggtcgtcg atgcgttgtg ggagaagtac ccccacacgc
     1981 agcttgaggc ttccggcacc cgcgtgggac tacccgccgg ccagatgggc aactccgagg
     2041 tagggcacct gaacataggc gccggcaggg tcgtctacca ggacctcgtg cgaatctccc
     2101 tggccgtcca ggacggcacc tttttcgaga acccggccct cctgcgagcg atcaaccacg
     2161 tgcgcgagca cgattcgaag ctgcacctgc tggggttgtt gggccctggc ggcgtccaca
     2221 gccacatcga tcatctctac gcgctgctca gcctggtagc caagcaccac gtgaagaagg
     2281 tggaggtcca cccgatactg gacgggcgcg acacgccccc ccagagcggc ctggggtaca
     2341 tcaaggagtt ggagcagcgg atcgccacgc tggggaccgg tcgaatcgcc acagtcgcgg
     2401 gccgctacta cacgatggat cgggataagc gctgggaccg cgtggagaag gcctacaggt
     2461 gcatggtgtt cggcgaaggg aacagcggac cgtcggcgga ggccatcgtc gagaagtcat
     2521 acgccgatgg tgtaacggac gagttcgtga tcccttcggt ggtgcgcgag acgccggaga
     2581 agggcgagga atcgctgatc cgcccccacg atgcgctgat tttcttcaac ttccggacgg
     2641 acaggacgag ggagatcacg cgggcactgg taatggacga cttcccgttc ttcaaccgcg
     2701 gtcccaagat cgagggcctc gacgtcatca ccatgacgga gtacgaagag gggctgccgg
     2761 tcacggttgc ctacccttcc gagttcctga acgaggtgct ggcagacgtg ctcgcggagc
     2821 acgacatctg ccagttccac acggccgaga ccgagaagta cgcccacgtc accttctttc
     2881 tcaacggtgg ccgtgaggaa ccattccccg aggaggacag gaagctcatc ccgtctccga
     2941 aggtcgctac ctacgatttg cagcctgaga tgagcgccca cgaggtgacc caggtcgtcc
     3001 tggacgtctt gaagcagggt caccaccagt tcatcatcat caactatgcc aatggggaca
     3061 tggtgggcca taccggcagc atcccagcag caattaaggc tgtggagacc gtggacgagg
     3121 gggtaggcaa gatcgtggat gccgtgctag cccatgccgg tgccgccctg gtaaccgcgg
     3181 accacggcaa cgccgaggag atgattgacc cggcgacggg cggcccatgg accgcgcaca
     3241 cgactaaccc tgtcccgttc atcctcgtct gcgagcggac caaagggatg aggctccgcg
     3301 acggaggggc gctcagtgac atcgcgccca caatcttcga cctgatgggc atccccaagc
     3361 ccgacttgat gacgggccgc agcctgattg taaagtagga gtgagtgagg agagacagct
     3421 tgtctcgtat cccgataatc gccggcaact ggaagatgaa taccaacctc gaggaggcgg
     3481 tggcgctcgt cgaggcgatg cggccacgcc tcgaagccat cagtggcgtc gaggtcgtgg
     3541 tctgtccccc tttcatttcc cttgcggccg tgcaggggca cctccatggc agtcctatcg
     3601 gcctgggtgc acaggacatg tacttcgcgg agaagggcgc ctacaccggg gagatcagtc
     3661 ccctgatgct ccgcggactc gtggactacg tgatcatcgg ccacagtgag cgccggcagt
     3721 acttcggcga gaccgacgag ctggtggcga agaaggtgaa ggcggcgctc gatcgtgggc
     3781 ttaagccgat catgtgtgtc ggcgagcggc tagaggagta tgaggccggc aagacccagg
     3841 aggtagtcac acgccaggtc cggggcggcc tgagcgggat cgagagcctc ggcgacctag
     3901 tagtcgcgta tgagcccgtg tgggccatcg gaacgggcaa ggcggcgacc ggccagggcg
     3961 ccaacgaggt gattgggatg atccgtcgac tcttcggctc caagttcggc tcggaagccg
     4021 catcagccct gcgcatccag tacggtggca gcgtcacacc ggacaacatc cgcgagttca
     4081 tggagcaacc agagatcgac ggtggcctgg ttggcggcgc tagcctcaag gcggatgcct
     4141 tcgtagagat cgttcgccag accgctgagg caaagagggc ctagccccaa tacggttcac
     4201 ttaagctaac aacatagaag aagggacgtc tccatcgagg tttgtggcat ccttggaagt
     4261 gtcgaaacca ggcttgccac ggccctcgaa aggaaccgtc ccttgggatt catgatacgt
     4321 cagatcgctc cgaacgccaa actcgccgat gaggtttcga tggaggccct ggaggcagca
     4381 gtgccccaag ctaccgtaga ggccgtaatc gctgatctgg gggtggccga gcagcgcagg
     4441 cgtaagctcc cggccgaact gaccctgctg ctgagtgtgg ccatgaacct gttcactcac
     4501 caccccctgg aggaggtgct gcgcaaactg ctcaggggtc tgcgcctgat ctggccggaa
     4561 gacgacttcg ccacagcagg caagggcggc atctccaaag cccgctatcg cctgggtgcg
     4621 gcccccttgg tggagctgtt ccatcgggtc tgtcagccta tggccaccga ggacacccca
     4681 ggagcctttc tcttcggcct gcgcctgatg gccctcgacg gcacgaacga ggacctgccc
     4741 gacacggtcg agaacgcccg cgtcttcggc cggcctggcg gcaaccgggg agacggggcc
     4801 ttcccccagg ccaaagggtt ctacttgtcg gagtgtaaga gtggatcgtg gtcatgaggc
     4861 cgcgggtgat tcccagagtg tcctggagca ccttggccac gggcacgagg cagttggtcg
     4921 tgcaggacgc gttggagatt atcctgtcct tggccgggtc atacttctcc tcgttcacgc
     4981 ccatgacaat ggtgacgtcc tcgcccttgg cgggtgccgt gatgatcacc ctcttggcgc
     5041 caccggacag gtgtgccttg gccttctccg cgtcggtaaa gatgccggtg gactcgatca
     5101 cgagctctgc cccgacatcg ccccagggga tctgggctgg atccttctgc gcgaacacct
     5161 ggattcgctt accattgacg acgagcgcgt tctcctgcgc ctccaccgtg ccagggaacc
     5221 gcccgtaagt ggagtcgtac ctgagaagga aagcgttggt ctgggtgtcg gtgaggtcgt
     5281 tgaccgccac gacctccagt tcgttcgggt gtctctccag cattgccttg agggattggc
     5341 gcccgatgcg cccgaatcca ttgatgccga ccttcactgc catgtgcttc tcctttcgtc
     5401 gccgaccgct attcgcgacc tgtttccctt cgatgccgat gtggtccggc ggaagatgcc
     5461 tgtggacctg tgcagagcta catgccgcga gtcgtagctc ggggcgaccc gcggtcgccc
     5521 cgaaagaacc agcttttccg tttcctgcaa gttccagtcc gcccgcttac tgccgcctta
     5581 tgttgtagaa ggcttcccgg cccgcataga gcgaattctc gcccaactgg tcctcgatgc
     5641 gcaacagctg gttgtacttt gctacccgct cgccgcgggc tggcgccccg gtcttgatct
     5701 gcccggcatt agtggccacc gagaggtcag cgatagtcgt gtcctccgtc tcgccggacc
     5761 gatgagacac taccgcggtg aaacccgctc tctgggccat ctggatcgcc tccagggtct
     5821 ccgtgagcgt gccgatctgg ttcagcttga tcaggatcga gttggcgctc ttctcctcga
     5881 tgccgcgctt gagccttgca gtgttggtga caaacaggtc atcccccacg atctgcacgc
     5941 ggtcgcctag ttccctcgtg agcatggccc agccctccca gtcgtcctcg gccaggccgt
     6001 cctcgatcga aatgatcggg tactgcctga cccagtcgga gtagaactgg accatctgct
     6061 cagatgtaag tgaacgcccc tcgcgcttga ggttgtactt gccgttctcg tatagctccg
     6121 atgctgccgg gtccagggcg aggaagactt cctcccccgg cttgtagccc gcggcgccga
     6181 tcgcttccag gatgacatcc actgcttcct ggttggacct caggctggga gcgaaccctc
     6241 cctcgtcgcc gacgttcgtg ttgagcccct tccccttcag caccttcttg agggcctggt
     6301 agatctcggc actcatccga agggcgctgg cgaagtccct cgctccaacc ggcatgacca
     6361 tgaactcctg gaggtcggtg gagttctccg cgtgctttcc gccgttcaag atgttcagca
     6421 tcgggaccgg cagcatgtgg gcgttagccc cgccaatgta ccggtagagc gggaggtcca
     6481 ggctattggc cgccgcgtga gcgcaggcaa gggagactcc gagcatggcg tttgcgccca
     6541 gcttgctctt gttcggggtg ccgtccatct cgatcatcat cctatcgatg ccgacctgat
     6601 cgagcacgtc catctcctgg agttcttccg cgatcaagtc cgtgatattc tcgatggctt
     6661 tgcggactcc ggctccccca aactgagtcg catccttgtc gcggagttcc agagcctcgt
     6721 gggcgccagt ggacgcgccg gaaggcacct cggcacgccc taccgtgccg tcttccagga
     6781 taacctcaac ttcaaccgtg gggttaccgc gcgaatccag gatctgccgg gcgaagacgt
     6841 cttctattac cgtcatagtc cctccgtgtt ctcaaaacag caaacagcga acaacgcttc
     6901 agcaggttgc gagccgcagg cgctcactac attgctttct cgctgtccgc tgtctgggga
     6961 agactaatag ccagtcgtta gtcgctactc ttgatagcta ggccgatgtc cttccggagt
     7021 tcctcgtagt tcagatggct ctccaggagc cgctcgtacc tcttgatctt cttctcctcg
     7081 tggaaccgca cctcggtaaa tgcctgctcc acgagggttg ccgcggtcaa agtgtcctgt
     7141 gaggcgacaa ccacgggcac ccccatctcc tcggccttgc ccaggatgat cgggctgggg
     7201 ttcaggttgc cggtgagcat cagacagccg gtcgaggtct ccagcgcggc tagctggatg
     7261 tccgggcggt cgccgccagt gatcaccacc ttatgctgct tgcggcggaa gtagctgagc
     7321 gcgttctcgc cgctcatggc tccgaccatc acggtctcca ccagcctgtc catgtgatcg
     7381 ctgccggaaa tcacccttcc gccgatccta tccaccagat cctggacggt gaccgccatc
     7441 agcatacggt cctcgggaat gatcgcgtag accttgatgc cgcgccggtt caggtatggc
     7501 gtcagactct ccgcgacgaa ctcgaccatt ccgcgcggaa cggcgttgaa gatgacgccg
     7561 gtcatccgat cgcgcatcca ggattttgcg gagaggatgc tgtcggcgat catctcatcc
     7621 ttggggcgga ccagcaggac gcactgggcg ccgagcatgt taaccgtcgt tttggcatcc
     7681 aggccaacca tgtagccctc ggagaggcca ctgacgccct ccacgaccat cacatccttg
     7741 ccggacgagg aaatcttgaa ggcatccatt accttctggc tatagtccac ggctcgatca
     7801 gagaggatcc agtccagggt ctccccgtcg acaaggaccg gatccaggat gtccatcgac
     7861 tccatgaggc ccaaggacct cttcatgata gcggcgcacg tattggcgct ctcgatgttc
     7921 cttgccgcct cggtcagggg ccgaatgtat ccaacgctga agccgtcatc agcgagcttc
     7981 ctggccaggc ctaggcagaa gactgtcttc ccggcgaagc tctccatcga catgacgtat
     8041 atggtcgcca tgttgagcta cctccctctc tctactctct ccatcagcac cggt
//
LOCUS       DATALS010000324         3131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_35004,
            whole genome shotgun sequence.
ACCESSION   DATALS010000324 DATALS010000000
VERSION     DATALS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35004"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="VHS06_07250"
     CDS             complement(<1..1487)
                     /locus_tag="VHS06_07250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2987950.1"
                     /translation="MVLVTLFGLALRLFALGHGLPNQYVPDESTMVGGALRMGATRSL
                     QPSTFIYPALMMYLFAAEYLGLLVVGRLSGLFASIEQFKEYAFVDPTLFYLLPRLAVA
                     LAGAATIPLVFLVGKRLYGRWAGVAAAALMAASVMHVQMSHQARHWVPVTLLTLVVLW
                     VSLDLAEKGRRRDYWLSGLLVGITAATSFNGFLVMGLPLMAHLMLLRKRGGRLMDWRL
                     HIPMAVPLLLAPAVFFALNPYILLQFNQFAAFQSKGDSSIGGQIRGHFENFFTEKGGF
                     PFFAEATLAYEPAITLLGFAGAVLAARRFQSTRALVLIYPVFHYFMFSTTAPSLEQRY
                     MLPAVAVWALPAGLAVSMAGERLYRRLGSGIGAATAATGISLLVLFLVSLPSLRYDWL
                     LAQTDTRTQAAEWIEHNIPAGSDLAVESYPPPLTADLETLRAQQQTEPKSLGSRDQWL
                     LEKGLPAGETAYRLTRLNLIDTTPTVDNLTPYLATHSHRYFVVTD"
     gene            complement(1617..2786)
                     /locus_tag="VHS06_07255"
     CDS             complement(1617..2786)
                     /locus_tag="VHS06_07255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2987951.1"
                     /translation="MPSISQFAKRAIAGKPALFGWLYQPYADYLFKKRRSYLAARYQE
                     LLHSDQRAEAGTAFSIGHEPTIRCNLACKMCYQGDFREDEGGVKELTLDQMIAVYDRV
                     NVSDAKLVGSEIFMRKDIYELLDYLWKRKIPTSLMTNGTLLNEEGIQKLRKYDNIEWV
                     GFSVDGSKEIHNAIRRRPYAFDRTVEAIGRTMPYFTVSVNSVILKENLDDLTSILRLC
                     KKLGIPFVHYIFEEAYSQAQVQKTHQILQSEMGWGPDDYEICTNVRDDFEYSFEELVS
                     AIDKLKREGRKLGVYPVFSPDVWSRRLSDYYFGTAAKNLHLVCAKLVSDTPTFRLDHL
                     GNVHHCGVIRRPFGNLLEQSPEEIWNSEEYRRYRKLLMGKNLLPICTRCCKAAYL"
     gene            2923..3111
                     /locus_tag="VHS06_07260"
     CDS             2923..3111
                     /locus_tag="VHS06_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987952.1"
                     /translation="MNQPSTSIVRPSDGVLSTVHTGLIARLLNRRLDLLSIAFLFGLS
                     LLLSIRQIVLGGVPLEYG"
BASE COUNT          581 a          918 c         1002 g          630 t
ORIGIN      
        1 aagtcggtca ccacgaagta gcggtgactg tgggtggcca ggtagggagt caggttgtcc
       61 accgtggggg tggtgtctat caggttaagc cgggtaagcc ggtaggccgt ctcgcccgcc
      121 gggagaccct tttccagcag ccattgatcc cgtgagccga ggctctttgg ctcggtctgc
      181 tgttgtgccc gcagcgtctc cagatcggcc gtgagcggcg gtgggtagga ctccactgcc
      241 agatcggacc ctgccgggat gttgtgctcg atccactcgg cggcttgtgt gcgggtgtca
      301 gtctgcgcca gcagccaatc gtagcgtagt gaaggcagcg acacaaggaa gagcaccagc
      361 agcgagatgc cggtagccgc tgtggcggca ccgatgccac ttcccaacct cctgtagagc
      421 ctttcgccgg ccatgctgac cgccaaccca gccggcagcg cccacacggc cacggccggg
      481 agcatgtagc gttgctccag actgggagcc gtggtggaga acatgaagta gtgaaagact
      541 gggtagatca gcaccagcgc tcgggtggat tggaaccgtc gagcggccag tacggccccc
      601 gcgaagccga gaagggttat tgcgggctca taggccagcg tggcctcggc gaagaagggg
      661 aagcctccct tctcggtgaa gaagttctcg aagtgtccac gaatctgtcc gccgatggac
      721 gagtcgccct tggactggaa ggcggcgaac tggttgaact ggagcaggat gtaggggttc
      781 agcgcgaaaa agaccgccgg cgccagcagc aggggaacgg ccatggggat gtgcagccgc
      841 cagtccatca gcctgccgcc ccgtttccgg agcagcatca gatgggccat cagtgggagg
      901 cccatcacca ggaagccgtt gaaggaggtg gccgcggtta ttcccaccag aagcccggag
      961 agccagtagt cacgacgacg acccttttcc gccaggtcga gggagaccca cagcaccacc
     1021 agggtgagta gggtcaccgg cacccagtgc cgcgcctggt gggacatctg gacgtgcatc
     1081 acgctcgcgg ccatcagcgc cgcggctgcg acacccgccc agcgaccgta cagccgcttt
     1141 ccgaccaaga agaccagcgg aatggtggcg gcgccggcca gcgccacggc gagccgaggc
     1201 agcaggtaga acagggtcgg gtctacgaat gcgtactcct tgaactgctc gatggaggcg
     1261 aagagccccg agagccgccc taccaccaga agccccagat actcggcggc gaagaggtac
     1321 atcatgagcg cagggtaaat gaatgtgctg ggttgaaggc tgcgggtggc gcccatgcgc
     1381 agtgcgccac ctaccatggt gctctcatcg ggcacgtact gattgggcag tccgtgaccc
     1441 agggcaaaca gccgtagcgc cagcccaaag agcgtcacca gcaccacggc caagcccacc
     1501 caatgggttc tgggttctgg gttctgagtt ctgggtgagt gttgagtgct gggggctgac
     1561 tgctgatagc tgatagctga tggcttgccg cggtctttcg ctcgtttatc catactctag
     1621 aggtacgccg ctttgcagca gcgcgtgcag atcggcagca gattcttgcc catcagcagc
     1681 tttcggtagc gcctgtactc ctcggagttc cagatctcct cgggcgactg ctcgagaagg
     1741 ttgccgaagg gtcgccggat cacgccgcag tggtgtacgt tgccgagatg gtccagccgg
     1801 aaggtggggg tatcgctcac cagcttcgcg cacacgaggt ggaggttctt ggcggccgtt
     1861 ccgaagtagt agtcggacag ccttcgagac cagacgtcgg gactgaagac gggatagacg
     1921 cccagtttgc gcccctcgcg cttcagtttg tcgatggccg atacgagttc ctcgaagctg
     1981 tactcgaagt cgtcccgcac gttggtgcag atttcgtagt cgtcgggccc ccaccccatc
     2041 tcgctttgca ggatctgatg agtcttctgc acctgggcct gtgagtacgc ctcttcgaat
     2101 atgtagtgca cgaaggggat gcccagcttc ttgcacagtc tcagtatcga ggtgagatcg
     2161 tccaggttct ccttcaggat gaccgagttg acgctgacgg tgaagtaggg catcgtccgc
     2221 ccgatggcct ccaccgttcg gtcaaaggcg taggggcgtc tgcggatggc gttgtggatc
     2281 tccttggagc cgtccacgga gaatccgacc cactcgatgt tgtcgtactt tcgcagcttc
     2341 tggatacctt cctcgttgag gagggtcccg ttggtcatca ggctcgtggg gatcttccgc
     2401 ttccagaggt agtcgagaag ctcgtagatg tctttccgca tgaagatctc gctgcccaca
     2461 agcttggcgt ccgacacgtt gaccctgtcg tagacggcga tcatttggtc gagggtgagt
     2521 tcctttacac cgccctcgtc ctcgcggaag tccccctgat agcacatctt gcacgccagg
     2581 ttgcagcgga tcgtgggctc gtgccctatg gagaacgcgg tgcctgcctc ggccctctga
     2641 tcgctatgca gcagttcttg atacctggca gccagatagc tgcgtctctt cttgaagagg
     2701 tagtcggcgt agggctggta aagccagccg aagagggcag gtttgcccgc gatggctctc
     2761 ttggcgaatt gtgagatgga tggcaattga ggctctccct tccggccgca gtatcctcaa
     2821 gcggcggtcg aaagtcaagg cggggcctcg tttcgttgtc tgcccgacat gccgatgcta
     2881 caatcccgct gccatcgatc catcttccag ggtgattctc agttgaacca gccatctacg
     2941 tctattgtgc gtccttccga cggggttcta tcgactgtcc atacagggct gattgcccgt
     3001 ctgctcaacc gccgcctgga cctgctttcc atcgcattcc ttttcggact gtcgctgctg
     3061 ctctcgatcc gccagatagt gctgggcggc gtacctctag agtatggata aacgagcgaa
     3121 agaccgcggc a
//
LOCUS       DATALS010000325         4105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_258842, whole genome shotgun sequence.
ACCESSION   DATALS010000325 DATALS010000000
VERSION     DATALS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258842"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..472
                     /locus_tag="VHS06_07265"
     CDS             104..472
                     /locus_tag="VHS06_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987953.1"
                     /translation="MAQYFEPNELDRAPARHLHRLEPGQVYFDLDHPEDGPFTATGDE
                     EVAPGANIVSQDDMSEEAWEQLVSAGWGETRPDARGDRAYDQGAFGEQENPREDTLNS
                     APPGVGKGFIPRTPGEERRR"
     gene            540..1019
                     /locus_tag="VHS06_07270"
     CDS             540..1019
                     /locus_tag="VHS06_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX2987954.1"
                     /translation="MEARRVSDSRSQLSQFMLAPESNPWGQVHGGSIMKLVDNVGGVA
                     AMRHCHMPVVTAQFVKMNFLQPVALGAFVTARASLTGVGRTSMEIRVTVEAENLLTGE
                     VVQAAKAEAVYVALGEDGRPAEVPRLILEADEARRPAEGTKQRRASGAAELAGPAMI"
     gene            1175..2458
                     /locus_tag="VHS06_07275"
     CDS             1175..2458
                     /locus_tag="VHS06_07275"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX2987955.1"
                     /translation="MAIVGVVGGQWGDEGKGKVIDLLAQRAELVIRSHGGNNAGHTVL
                     NEQGKFALHLVPAGIFNATTTCIVGPGVVLDPAILLEELDGLNQRGVDTSGLMISSRA
                     HMVMPYHRLLDRLQEESRGAGRIGTTGRGIGPAYADKTSRDGLRVGELLDLDSFRVRL
                     EQTLAQKNRLLERVYEAAPFDVEAMYADFSGYAAQLRPYIKDTEPVVFDAVRSGKNIL
                     LEGAHGTMLDTDHGTYPYVTSSSSTVAGLCLGGGIGPTKLDVAVGVFKAYSTRVGGGP
                     MPTELKDAVGDHLRDRGYEFGTTTGRPRRCGWFDAVAAKHSVMVNGFNSLAITKLDVL
                     DDFSELKICVAYKLDGEVIDFVPADAGQLDRCVPVFETMAGWNCPLSSARGFKDLPAA
                     AASYLDRIERLLEVEIDVVGVGAAREAVICRRDLY"
     gene            2464..3117
                     /locus_tag="VHS06_07280"
     CDS             2464..3117
                     /locus_tag="VHS06_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX2987956.1"
                     /translation="MSIKYLLFDLDGTLIDTTDLIFNCYKSSVNRFVENPPDETEILK
                     GFGRPLTDQLWRMYPTLRDRIDEMAELWRKAQSELHDSLIKSFPATSETLRELKKRGY
                     PMGIVTSKERPMAVRGMGLNGLDNLMDVMVCVDDTSNHKPHPEPILKGIELLGAVPEE
                     TLYVGDSLHDMVAGRAAGVQVGAAMWGPFAKGPLVEFKPDFLLPKVTQLLEICPPMK"
     gene            3400..>4105
                     /locus_tag="VHS06_07285"
     CDS             3400..>4105
                     /locus_tag="VHS06_07285"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX2987957.1"
                     /translation="MSTDVRTALSFDTRTVLKENEVFLVCDASGDIRALSREGQGLYF
                     RDTRFLSMFEMSVDEVDISLLSSAGDQSFMGNLQYANNPGILKNGRKVGARTISVRRN
                     RFIRDGLHERIGFFSFNPFPVELTVRLSLGSDFRDMFDVRGYAKRARHGTIALPTVEG
                     DSIWLNYQGLDGIVRRTQISFETPPTGVELFPGEIETDQPDHSNGSTDHRSDVRVKPP
                     HAVATFSFVLPPREPIS"
BASE COUNT          790 a         1146 c         1334 g          835 t
ORIGIN      
        1 cctcacaggg cacggtaatt gcccccgctc gcccctaccg cctctgctcg ctcggagatg
       61 gatgggcaca ggggcgactc ttagttcgaa agaggtgagc agcgtggccc agtatttcga
      121 acccaacgaa ctggatagag cgccggctcg tcacctgcac aggcttgagc ccggccaggt
      181 ctacttcgat ctcgaccacc ccgaagatgg gcctttcacg gcaaccggcg acgaagaggt
      241 tgcgccgggc gccaacattg tgtctcagga cgacatgagt gaggaagcgt gggagcagct
      301 cgtgtcggcg ggttggggcg agacgcggcc cgatgccagg ggtgatcgag cttatgacca
      361 gggagctttc ggagaacagg aaaacccgcg agaggatacc ttgaactcgg cccctcctgg
      421 ggttggcaag gggttcatac caaggactcc gggagaggaa cgtcgtcgct gagcttgccc
      481 cgcactgtac cttccgcgct atactgtaga ccaatactgg gtctcggagg cgtggtcaat
      541 tggaggctag gcgggtcagc gactctagga gccaactgag tcagttcatg ctggccccgg
      601 agagcaatcc ctggggacag gttcacggcg ggtccatcat gaagctcgtc gacaacgtcg
      661 gcggggtggc ggcgatgcgt cattgccaca tgcctgttgt gacggctcaa ttcgtcaaga
      721 tgaatttcct tcaacccgtg gctctcgggg ccttcgtcac tgctagggct tcattaaccg
      781 gcgtgggcag aacctccatg gagattcgtg tcacggtgga agcggagaac cttctcaccg
      841 gagaggtggt tcaagcggcg aaggcagagg ccgtgtacgt ggcccttggt gaagacggcc
      901 ggcctgcgga ggttccgcgc ctcattttgg aggccgacga ggcgcgacgg cccgccgagg
      961 ggacgaagca acgacgggcg tctggggccg cggagttggc cggcccagct atgatataat
     1021 gcgatttggg gctcgtctta gccccatctc cggaactctt tcagtcaagc tgttaggggt
     1081 cggccaggcg ccgaccctta cttgcgtcgg ggatacgatt tccatcccgc catcggatga
     1141 atgccggggg cgtggggtag aggagacaac ttccatggct attgtgggcg tcgtcggagg
     1201 ccaatggggt gacgagggaa aggggaaggt cattgacctg ctcgcgcagc gggccgagtt
     1261 ggtgatccgc tcccatggcg gcaacaacgc tggccacact gtactgaacg aacaggggaa
     1321 gttcgccctg catctggtgc cagcgggcat cttcaacgcc acgaccacct gcattgtcgg
     1381 cccgggggtt gtcctcgatc cggccatcct gttggaggag ctggacggtc tcaatcagcg
     1441 tggggttgat accagcggac tgatgatcag cagcagggcc catatggtga tgccctatca
     1501 ccgattgctg gatcggttgc aggaggaaag caggggggcg ggccgtatcg ggacgaccgg
     1561 taggggcatc ggccctgcct atgccgacaa gacctcccgc gacgggttgc gtgtgggtga
     1621 actgctcgat ctcgactcct tcagggtccg tttggagcag acgttagccc agaagaaccg
     1681 gctgctggag cgggtgtacg aggcggctcc gtttgacgtt gaggcgatgt atgccgactt
     1741 ctcaggctac gccgcgcaac tcagaccgta catcaaggac acggaaccgg tggtctttga
     1801 cgccgtgcgc agcgggaaga acatcctgct ggagggtgct cacggtacca tgctggatac
     1861 cgaccatggt acctatccat acgtgacctc atcatcctcc accgttgcgg ggctctgtct
     1921 gggtggcggc ataggtccta ccaagctcga cgttgcggtg ggcgtattca aggcatactc
     1981 cactcgggtc ggcgggggac cgatgcccac ggagttgaag gatgcggttg gagatcatct
     2041 gcgtgatcgt ggatatgagt tcggcaccac cacgggacgg ccgcgccgtt gtggatggtt
     2101 cgatgcggta gctgctaagc attcggtgat ggtgaacggc ttcaactccc tggcgatcac
     2161 gaagctcgac gttctggacg acttctccga gttgaagatc tgcgtggcct acaagttgga
     2221 tggcgaagta atcgacttcg tgcctgctga tgccggacag ttggaccgct gcgtgccggt
     2281 gttcgagact atggccggct ggaactgccc tctctcctca gcgagaggct tcaaggatct
     2341 gcccgcggct gccgctagct acctggatcg gatcgagaga ctgttggagg ttgaaataga
     2401 cgtcgtcggg gtgggggctg cccgggaagc ggtcatctgc cgtcgtgatc tctattagat
     2461 cgtatgagca tcaagtatct gctgtttgac cttgatggaa cccttattga cacaactgat
     2521 ctgatattca actgctacaa gagttcggtc aatcggttcg tggagaaccc tccagatgag
     2581 acggaaatcc tcaagggctt cgggcggccg ctcactgacc agctatggag gatgtacccg
     2641 acgctaaggg atcggatcga cgagatggcc gaactctggc gaaaggctca gtctgagctt
     2701 cacgatagcc tgatcaagtc ctttcctgcg acttcggaaa cgcttcggga actgaagaag
     2761 cgaggctatc cgatgggaat agtgacctcc aaggagcgtc ccatggccgt gcggggcatg
     2821 ggtctgaacg gcctggacaa cctgatggac gtcatggtct gcgtggacga caccagcaac
     2881 cacaagccgc acccggagcc gatcctgaag gggatcgagt tgctgggtgc cgttcccgaa
     2941 gagaccctgt atgtgggtga cagcctgcac gacatggtcg cggggcgcgc ggcgggcgtg
     3001 caggtgggtg ctgcgatgtg ggggcccttt gccaagggac cgctggtgga gttcaagccc
     3061 gacttccttc tgccaaaggt cacccagctg ttggaaatct gcccgcccat gaagtagcgc
     3121 ttgaccgatg ttcttgtggc cttcagtcta ggagtcggcg gcgtcccgcg acattaggag
     3181 tcgagcgtga ccgcgacgcc acgctcgcta ccgtcgcgtg ctcgctcgcg ttgcggggac
     3241 gccccgggct cctggagatc gggactaccg cgtcagagga ctagagggca ggttggcgtg
     3301 gcgatgggtg atgcagatgc gatatcctta ctacagtgat agtagagcgt aaccggttgc
     3361 tcatgcccat tgctggaagc tgatgactga aagctgataa tgtccactga cgtacgaact
     3421 gccctttcct tcgatacccg gaccgttctg aaagagaatg aggtttttct agtctgtgac
     3481 gcctccggcg atatccgcgc tctgagcagg gaggggcagg gactctattt ccgcgatacc
     3541 cgtttcctca gcatgttcga gatgtctgtc gacgaggtcg acatctccct gctctcctcg
     3601 gcgggcgacc agagcttcat ggggaatctg cagtacgcca acaaccccgg catcctcaag
     3661 aacggccgga aggtgggcgc gcgcaccatc agcgttcgcc gcaatcgatt catccgagat
     3721 ggcctccacg agcggatagg cttcttctcc ttcaacccgt ttccggtcga gctgaccgtg
     3781 aggctctcct tagggtccga ttttcgagac atgttcgacg tgcgcggcta tgccaagcgt
     3841 gcccgacatg gcactatcgc tttgcccacg gtggagggcg actccatctg gctcaactat
     3901 caagggttgg acgggatagt gcgacggact cagatcagct tcgagacgcc gccgactggg
     3961 gtcgagctgt ttcctggaga gatagagacc gatcagcccg atcactcgaa tggatcaacc
     4021 gatcacagat ccgacgtgcg tgtgaagccg cctcacgcgg tggccacttt ctcctttgtc
     4081 cttccgcctc gggagccgat ctcta
//
LOCUS       DATALS010000326         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_391920, whole genome shotgun sequence.
ACCESSION   DATALS010000326 DATALS010000000
VERSION     DATALS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391920"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VHS06_07290"
     CDS             complement(<1..443)
                     /locus_tag="VHS06_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987958.1"
                     /translation="MRLITFLLMMVVAIGGTLGGYRLATGRDLINLSEYGLAPQPAVS
                     IGAAPQKAPTVQVVPPTAVPSPTATRPTPTPIPELPEKMLVGNTGGEGVFLRKTPHIE
                     LGDAHELRLNSQQVLCGDVSLASIGGSNFEPLQTVGILRLAEDLHG"
     gene            complement(551..2074)
                     /locus_tag="VHS06_07295"
     CDS             complement(551..2074)
                     /locus_tag="VHS06_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX2987959.1"
                     /translation="MAIINEKQRLNLAADELISVSVDASELSVASAEPLRTILSRYGR
                     PVSARAFADFQQSSLSDLAVQLYQMGFTLVHCPTWPNGDGTPKPIVSDIMAQDLRDQL
                     EQLDDDVETYILVATSREYIAVANSLRRHERRVVVVAEETKACRELRLCADEFVALPP
                     TRPERASEPARSRRSVETARVERVSEVARTEKPAEAVTAEPAPAPVRVERSARGSRAA
                     RPAEPVRSVEPVRPAEPARAERAEAADQLRLPSDVEVLAEVRRIVAAEGICTPRRLAR
                     ALCPVDRTPTGELRSRIANRIQSLIDAGKLQRERLVVGGTSVETILVREERGEKPSAA
                     TPESASVPTPAQAPTPTSKGGTLPPGVFALESVVASSAVPEVPAELPTTDYGKSGGSA
                     ARDLDAFVESVLRGPELAVGPTPENGERKEEPVAAEVAPAFVTQPVAESQPEANSEPA
                     VAAFEESPAAEKPAEAGEPVVAVEAAPAEPVKRPRTRRRSSPKKAAAAAEAVETAQQ"
     gene            complement(2174..>3533)
                     /locus_tag="VHS06_07300"
     CDS             complement(2174..>3533)
                     /locus_tag="VHS06_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX2987960.1"
                     /translation="CQAYAELEAEGYLVGRHGSGSYVSSDLPEDPSPSPVGEVVSTRA
                     EGVEASEPRQPTLPGTVARYSPASESIGVAFGEGKTLAALRQGPPRGLPFDFHPGQGA
                     WDGFPVGTWRRLLARQWRRGWRETMDYGDPAGYRPLREEVASYLARSRAVHCTADEVV
                     IVNGTQQALDLLSRVLLSPGGAVAVEDPGYPSARPLFAAYRARLLPIEVDQEGMVVDR
                     LRGSGARLALVTPSHQFPTGALLTLPRRLSLLGWARAEGGLVVEDDYDSGFRFEGRPL
                     ASLQGLDSSGRVVYLGSFSRVLFPSLRVGYAVLPPGLVGPFVEAKELTDRQTPILEQQ
                     LLAGFLGEGHFERHLRRMRQLYRRRREALVEALRRRLEGKVQAVGAPAGMHLMVRLLL
                     PMEEDEVVARAAAFGVAVYPAGPYYTTDNHQPALVIGYAGMDEERIVDGIDRLSRSLE
                     GC"
BASE COUNT          689 a         1190 c         1036 g          618 t
ORIGIN      
        1 ccatggagat cctcggccaa ccggaggatt ccgacggtct gaagtggttc aaagttcgag
       61 cccccgatgg aggcgaggga tacatccccg cacagtacct gctggctgtt cagccgtaac
      121 tcgtgagcat ctcccaactc gatgtgtggg gtcttgcgga gaaagactcc ctcgcccccc
      181 gtgttcccta ccagcatctt ctcggggagt tccgggattg gggtgggggt cggtctagtg
      241 gcagtagggc tgggaacagc ggtcggcgga actacctgca ccgtcggcgc cttctgtggg
      301 gcggctccaa tcgagactgc cggctgaggg gcaaggccat attctgagag gttgatgaga
      361 tcgcgacccg tggcaagtcg atagccgccc agcgtcccac ctatggcaac caccatcatc
      421 agcaggaagg tgatcagtct cacagggtag cgcctcctct aaaatcaacg agggggctgc
      481 cgatggaata ccggacagcc cccttgatca acctctagtc ggaactacga tgtaccgctt
      541 ctttgttctc ctactgctga gccgtctcga cagcttccgc cgccgcggca gccttcttgg
      601 gggacgagcg acggcgggtg cgaggtcgct tgaccggttc ggccggagcg gcttcgactg
      661 caaccacggg ctcccccgcc tctgcaggct tctcagccgc aggcgattcc tcgaatgcgg
      721 cgacagccgg ctcagaattc gcttccggct gagactcggc aacgggctga gtcacgaaag
      781 caggtgcaac ctcagccgcc actggctctt ccttgcgctc cccgttctcg ggagtagggc
      841 cgacggccag ctcaggtccc ctcagtactg actccacgaa ggcatccagg tcgcgagccg
      901 cggacccacc actcttgcca tagtccgtgg ttggcaactc ggccggaacc tcaggaaccg
      961 cggatgatgc aacgacactc tccagcgcga acacgcctgg aggcagtgtc ccacccttgc
     1021 tcgttggcgt gggtgcctgc gctggggtcg gaacggatgc cgattctggt gtagccgcgg
     1081 aaggcttctc gccccgctcc tcgcgaacca gaatggtctc cacagacgtt ccgcccacaa
     1141 ccagcctctc tcgctgcagc ttgcctgcat cgatcagcga ctggatccga ttggcaattc
     1201 gagatcgcaa ctcaccggtg ggagttcgat ccacggggca cagcgccctg gcaagacgcc
     1261 tcggggtgca gatgccctcc gccgctacaa tccgccgaac ctcggcgaga acctccacat
     1321 cgctgggcag ccgcaactgg tctgcagctt cagcgcgctc ggctcgggca ggctcagcag
     1381 gacgaacggg ctcgacagag cgaacaggct cggcagggcg agccgcacgg gaacctctgg
     1441 ctgaacgctc gacgcgtacc ggagcaggtg ccggctctgc tgtaaccgct tccgcgggct
     1501 tctccgttct cgctacctca gacacacgct cgactctagc agtttccaca gaccgcctgg
     1561 accgtgccgg ttcgctggct ctctcggggc gagtaggggg caacgccacg aactcgtcgg
     1621 cacagagccg caactctcgg caagccttgg tctcctcagc caccaccacc acacgccgct
     1681 cgtgacgacg caaagagttc gccacagcga tgtactcacg gctggtagct acgaggatgt
     1741 acgtctccac atcatcgtcc aactgttcca gttggtcgcg aagatcctgc gccatgatgt
     1801 cggagactat cggcttcgga gtgccgtcgc cattcggcca ggtggggcag tgcaccaacg
     1861 taaagcccat ctggtacagt tggacggcca ggtcggagag ggaggactgc tggaagtcgg
     1921 cgaaggcccg ggcactaacg ggacgcccgt aacggctgag gatggtcctc agtggctcag
     1981 cagaggcgac tgacagctcg ctcgcatcga cgctcacgct gatgagttcg tcagcggcca
     2041 ggttcagtct ctgcttctcg ttgatgatcg ccataaatcg atcgacttct ccgaactttc
     2101 agtttgtcct attgactcca ggatctagac aaattatagc gcaacaaccg cccaaagtcc
     2161 attctagcca acttcaacaa ccttccagcg accgcgacag ccggtcaatc ccatcgacaa
     2221 tcctctcctc atccatccct gcgtagccaa tcaccagcgc aggttgatgg ttgtcggtgg
     2281 tgtagtaggg accggcggga tatacggcga ctccaaaggc cgcggccctc gccaccactt
     2341 cgtcttcctc catgggaagc agcagtctca ccatcagatg catcccggcg ggagccccga
     2401 ccgcctgtac cttcccttcc agcctcctcc tcagcgcctc caccaaagcc tcccgccgtc
     2461 gcctgtatag ctggcgcatc ctccgcaagt gtcgctcgaa gtgcccctcc cccagaaagc
     2521 cagccaaaag ctgctgttcc aggatcggag tctgtcgatc cgtcaactcc ttcgcttcca
     2581 cgaagggtcc gaccagtcca gggggcagta ccgcgtagcc aacccgcagc gagggaaaca
     2641 gcaccctgga gaaactgccg aggtaaacga cacggcccga cgagtccaga ccctggagag
     2701 atgcaagcgg acgtccctcg aacctgaagc cgctatcgta gtcgtcctca accaccaatc
     2761 ccccttcggc tcgggcccaa cccaacaggg agagtctcct cggcagcgta agcagggcac
     2821 ccgtgggaaa ttggtgggag ggggtgacaa gggccagcct ggcgccgcta ccgcggagtc
     2881 ggtccactac cattccctcc tggtccacct caatcggcaa gaggcgagcg cggtatgccg
     2941 cgaaaagcgg acgcgccgac gggtagccgg gatcctccac ggcaacggcg cccccgggac
     3001 tcaaaagcac cctggagagc agatccaaag cctgctgggt accgttgact atcaccactt
     3061 catcggccgt gcaatgcact gccctggaac gagccaggta ggaggccacc tcctcacgca
     3121 gcgggcggta cccggcgggg tcaccgtagt ccatagtctc ccgccaacca cgccgccact
     3181 gccgcgccaa caggcgccgc caggttccga ctgggaagcc atcccatgca ccctggcccg
     3241 gatggaagtc gaatggaagg ccacgcggag gaccttggcg caaagcggca agggtctttc
     3301 cttccccaaa ggcgaccccg atcgattcag atgccgggga atatcgcgcg accgtgcctg
     3361 gtagggtggg ttgtcttggc tcgctcgcct ccaccccctc cgcgcgggtt gagacaacct
     3421 cgcccaccgg agagggtgat ggatcttctg ggagatcgct agagacgtaa ctcccagagc
     3481 catgcctgcc caccaggtag ccctcggcct ccaactcagc gtaagcctga cag
//
LOCUS       DATALS010000327         4965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_169102, whole genome shotgun sequence.
ACCESSION   DATALS010000327 DATALS010000000
VERSION     DATALS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169102"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VHS06_07305"
     CDS             <1..146
                     /locus_tag="VHS06_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX2987961.1"
                     /translation="SCNTRTQYINPLPPVMEPGEFYMPYPVEVQVGGATVFVLNVERW
                     ERL"
     gene            296..1093
                     /locus_tag="VHS06_07310"
     CDS             296..1093
                     /locus_tag="VHS06_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEX2987962.1"
                     /translation="MTGTLINVAAVLIGGTVGALAGERLPSRYRETVLHAIGLMTILI
                     GLQMALGTKSAIIVLGSVVVGAILGEAARIDDGLSWLGDWVEARVMGAGGRGPGVGQV
                     SPDTRHPIPDTTGGGANRAKPEGGTGKGASLFSKGFVTASLVFCVGPMSILGSFQDGL
                     TGVHDTLAVKSVLDCFTAIALASSLGWGVVCSAVIVLLYQGSLTMSAVWAKPFMTEAV
                     VTEMTAAGGLLIVGIGLNILGLMKIRVANMLPALIVAPILVALVGGR"
     gene            1123..2766
                     /locus_tag="VHS06_07315"
     CDS             1123..2766
                     /locus_tag="VHS06_07315"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX2987963.1"
                     /translation="MTKYIFVTGGVVSSVGKGITVASLGRVLKSRGITVSVQKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDVSLTRASNVTSGQIYSGVIGKE
                     RRGDFLGGTIQVIPHITNEIKERISRVAKQTGAQVVIVEVGGTVGDIESQPFLEAIRQ
                     MRREAGEDNTLYIHLTLLPYIGSTHELKTKPTQHSVKELRGIGIQPDVIVCRSDYPVS
                     EDLKEKIALFCNVEKRAVVPLETVPSIYQVPVILEEAGLGDFVVERLGLPERPAELTE
                     WRKMVDRSQGLKDTLNVGVVGKYVGLHDSYLSVKEALFHAGLHENRRINLEWIDSEDL
                     EREGDGRLKELNGIVVPGGFGYRGVEGMVLAARYAREHDIPYFGLCLGMQIICIELAR
                     FVLGTTEPNSTEFNMFTAHPVIDLLPEQRDVGEKGGTMRLGQYPCQLVPGTRAAEAYG
                     TANIIFERHRHRYEFNNDYRELLGVAGLVFSGLSPDGRLVEIGELAGHPWMLGTQFHP
                     EFKSRPNRPHPLFSAFIRACKVSLREGDQRPLPLEKLNS"
     gene            complement(2693..3595)
                     /gene="glpX"
                     /locus_tag="VHS06_07320"
     CDS             complement(2693..3595)
                     /gene="glpX"
                     /locus_tag="VHS06_07320"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX2987964.1"
                     /translation="MGEPIGRNLGFDLVRVTESAAMSAAQWQGRGDKNAIDGAAVEAM
                     RYTLQNVEMDGVVVIGEGEKDKAPMLYIGEQIGTGKPPMVDVAVDPVDGTTLTSKGLP
                     NAISVVALASRGSMFYAPGIVYMEKIAVGPRGKGCISLDATVEENIRCVAKAKDLEIG
                     EVTVVVLDRPRHEDLIRQIREAGARIKLIGDGDVAGALEAASEGSGVDILMGIGGSPE
                     AVIAACAMKCLGGEIQCRMAPRNDEERQKAVAAGFDPDKILTTDDLVKDDDVFFAMTG
                     VTNSVSPTAAGADRPREGSPCKPE"
     gene            complement(3773..4420)
                     /gene="fsa"
                     /locus_tag="VHS06_07325"
     CDS             complement(3773..4420)
                     /gene="fsa"
                     /locus_tag="VHS06_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX2987965.1"
                     /translation="MELFVDSADIKEIEEAVGWGIISGVTTNPSLIAKEGKVDFKTVV
                     QQICNMVQGPTSAEVISLDTEGMLKEAREIATWSPHVVIKIPMTWDGIRAAKVLSREG
                     VKTNVTLVFSPNQALLAGLAGATYVSPFLGRLDDAGHDGMSVVGEIVEIYRKQGIQTK
                     VLAASIRHPLHIVQAAKIGADVATVPFKVLKMMVKHPLTDAGIARFLEDWKKVVG"
     gene            complement(4433..>4965)
                     /locus_tag="VHS06_07330"
     CDS             complement(4433..>4965)
                     /locus_tag="VHS06_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX2987966.1"
                     /translation="HDIPYFGLCLGMQIICIELARFVLGTTEPNSTEFNMFTAHPVID
                     LLPEQRDVGEKGGTMRLGQYPCQLVPGTRAAEAYGTANIIFERHRHRYEFNNDYRELL
                     GVAGLVFSGLSPDGRLVEIGELAGHPWMLGTQFHPEFKSRPNRPHPLFSAFIRACKVS
                     LREGDQRPLPLEKLNS"
BASE COUNT          944 a         1512 c         1521 g          988 t
ORIGIN      
        1 ctagttgcaa cacccgcacg cagtacatta acccccttcc acccgtgatg gagccgggcg
       61 agttctatat gccgtaccct gtcgaggtac aggtgggcgg ggccacggtc ttcgtactga
      121 atgttgagcg ttgggagcgg ctttagcttc aacgcgtcct cgccgaacag ccattgcccc
      181 ggtccagggg ctgcagcggc atcgagcttc ccaagcaagt attggcctta agtagggccg
      241 agccgtgaga ggcctggggt ctccacgtgt gtctgagggg gcggagaggt ctgagttgac
      301 cggaacactc atcaatgtgg ccgcggtgct gataggtggg accgtcggtg cgctggcagg
      361 cgagcgattg ccgtctaggt acagagagac ggttctgcac gctatcggcc taatgaccat
      421 cttgatcgga ctccaaatgg cgctcggtac taagagcgcc atcatcgtgc ttgggagtgt
      481 cgtggttggt gcgatcctgg gagaggccgc aaggatagac gatggcctct cctggctcgg
      541 agattgggtt gaagcgcggg taatgggggc cgggggtcgg gggccagggg tcgggcaagt
      601 ttctcccgac acccggcacc cgatacccga cactacgggg ggtggcgcga atcgtgcaaa
      661 gccagaaggt ggaacaggaa aaggcgcctc cctcttcagc aaaggttttg tcactgcgag
      721 cctcgttttc tgcgtgggac ccatgagtat tctcggttcg tttcaggacg gactgacggg
      781 cgtccacgat accctggcgg tgaagtcggt tttggattgc ttcacggcca tagccctcgc
      841 ctcatccctc gggtggggag tggtgtgttc tgccgtgatc gtgctgctgt accaggggtc
      901 gttgaccatg agcgcagtct gggccaagcc attcatgacc gaggccgttg tgacggagat
      961 gaccgccgcg ggcggtctgt tgatcgtggg gatcgggttg aacattttgg gactcatgaa
     1021 aatccgagtc gccaatatgt tgccggccct gatagtcgct cccatcctgg tggcgctggt
     1081 tgggggcagg taaacacgca cgctcgaatg tgggagtagc cgatgaccaa gtacattttc
     1141 gttaccggtg gtgtggtgtc ctccgttggt aaggggatta ccgtcgcctc tttgggacgg
     1201 gtgctgaaga gccggggcat taccgtctcg gttcagaagc tcgacccata catcaacgtg
     1261 gacccaggca ccatgagccc cttccagcac ggtgaggtgt tcgtcactga cgatggcgcc
     1321 gagaccgacc tggatctggg gcactatgag cgtttcatag acgtcagcct caccagggcc
     1381 agcaatgtca cttccggcca gatctacagc ggggtgatcg gcaaggagcg acggggcgat
     1441 ttcctcggag gcaccattca ggtcatcccg cacatcacca acgagatcaa ggaacggatc
     1501 tccagggtag ccaaacagac cggcgcacag gtcgtgatcg tggaggtggg cggcaccgtt
     1561 ggagacatcg agagccagcc cttcctggaa gcgatccgcc agatgcgccg agaagccggc
     1621 gaagacaaca cactctatat ccacctcacc ctgctgccct acatcggctc cacccacgag
     1681 ttgaagacca agcccaccca gcactccgtc aaggagctga ggggcatcgg catccagccg
     1741 gacgtcattg tctgcaggtc ggactatccc gttagcgagg atctgaagga gaagattgcc
     1801 ctcttctgca acgtggagaa gcgggcggtg gtgccgctgg agaccgtccc cagcatctac
     1861 caggtgcctg tgatcctgga ggaggccgga ttgggcgact tcgtggtgga gcggctgggg
     1921 ctgccggagc ggcccgccga actcaccgag tggcgcaaga tggtggaccg tagccaggga
     1981 ctgaaggata cgctgaacgt gggtgtcgtg ggcaagtatg ttggcttgca cgactcctac
     2041 ctgagcgtga aagaggccct cttccacgcg gggctgcacg agaacaggcg catcaatctg
     2101 gagtggatcg actccgaaga tctggagcga gagggcgacg gccggctcaa ggaattaaac
     2161 gggatcgtgg ttcccggcgg cttcggctac aggggcgtcg aggggatggt cctggcggca
     2221 cgctacgccc gcgagcacga catcccctac tttgggcttt gtctggggat gcagatcatc
     2281 tgcattgagc tggcgcgttt cgtgctggga acaacagagc cgaatagtac cgagttcaac
     2341 atgttcaccg ctcatcccgt gatcgacctg ctgcccgagc agcgggacgt gggtgagaag
     2401 ggcggcacca tgcgactggg gcagtaccca tgtcagctgg tgcctgggac ccgggccgca
     2461 gaggcctacg gtacggccaa catcatcttc gagcggcata ggcatcgcta cgagtttaac
     2521 aacgactacc gtgagctgtt gggagtggcc ggactggtgt tcagcggtct ctcccctgat
     2581 gggcggttgg tagagatcgg agagttggca gggcacccct ggatgctggg cactcagttc
     2641 catccggagt tcaagtcccg ccccaaccga ccacatcccc tcttctcggc tttcattcgg
     2701 gcttgcaagg tgagccttcg cgagggcgat cagcgcccgc tgccgttgga gaaactgaat
     2761 tcgtaacgcc ggtcatggca aagaagacgt catcatcctt gacgagatcg tcagtggtga
     2821 ggatcttgtc tgggtcgaag ccggccgcga cagccttctg gcgctcctca tcgttccgag
     2881 gagccattcg gcactggatc tcgccgccca agcacttcat cgcacaggcc gctatgacag
     2941 cctccggcga gccaccgatg cccataagga tatccacgcc ggacccctcg ctggccgctt
     3001 ctagcgcgcc cgccacgtcg ccgtcgccga tcagcttgat tcgtgctccg gcctctcgaa
     3061 tctggcgaat cagatcctcg tgccgcgggc ggtccagcac caccaccgtg acttcgccaa
     3121 tctccaagtc tttggccttg gcgacacaac gaatgttctc ctccaccgtg gcatccaggc
     3181 tgatgcaacc cttgccccga ggccccacgg cgatcttctc catatagacg atgccgggcg
     3241 cgtagaacat gctcccgcgg gaggccagcg cgaccacgga gatagcgttg ggaaggccct
     3301 tagaggtcag tgtcgtgccg tccacggggt cgacggcaac atccaccatc ggaggcttcc
     3361 cggtcccgat ctgctcgccg atgtagagca tcggagcctt gtccttctcg ccctccccga
     3421 tcaccaccac gccatccatc tcgacattct gcagggtata gcgcatagcc tcgacggcgg
     3481 caccgtctat ggcgttcttg tccccgcggc cctgccattg ggccgcggac atggccgcgg
     3541 actcggtgac tcttactaga tcaaacccca ggtttcgacc aattggctcg cccatggaca
     3601 tgcctccaac gtagcgctta tcgcagagtt atcagcggct agcccctggc taaccgtcgc
     3661 cgcctttcag cagtcctgac agtaaggtac gctacccctg cagttcatcc actgaccgag
     3721 ctctagagca ccctgtcccc gccactgatt ccagacggcc gattgccgga atttaaccca
     3781 ctaccttctt ccagtcctca aggaagcgtg cgatcccggc gtccgtaagc gggtgcttga
     3841 ccatcatctt gagaaccttg aacgggaccg tcgccacatc cgcgccgatc ttcgccgcct
     3901 ggacgatgtg gagcggatgc ctgatgctgg cggccagcac cttggtctgt atcccttgct
     3961 tgcgatagat ctcgacgatc tcccccacga ccgacatgcc gtcgtgtccc gcgtcgtcca
     4021 accttcccag gaatgggctg acataggtcg caccggcaag cccggccagt aacgcctgat
     4081 tgggggagaa cacgagggtt acgttcgtct tgaccccctc cctggagagc accttggcag
     4141 ccctgatccc gtcccaggtc atagggatct tgatgaccac gtggggagac caggtagcga
     4201 tctcgcgagc ctccttcagc atcccctcgg tatccaggct gatcacctcg gcgctggtcg
     4261 ggccctggac catgttgcag atctgctgca ccacagtctt gaagtcgacc ttgccctctt
     4321 tcgcgattag actcgggttg gtggtaacgc cgctgataat tccccagcca acggcttctt
     4381 cgatttcctt gatgtcggcc gagtcgacga aaagctccat tgcccagact ccttacgaat
     4441 tcagtttctc caacggcagc gggcgctgat cgccctcgcg aaggctcacc ttgcaagccc
     4501 gaatgaaagc cgagaagagg ggatggggtc ggttggggcg ggacttgaac tccggatgga
     4561 actgagtgcc aagcatccag gggtgtcctg ccaactctcc gatctctacc aaccgcccat
     4621 caggggagag accgctgaac accagtccgg ccactcccaa cagctcacgg tagtcgttgt
     4681 tgaactcgta gcgatgccta tgccgctcga agatgatgtt ggccgtaccg taggcctccg
     4741 cggcccgggt cccaggcacc agctgacatg ggtactgccc cagtcgcatg gtgccgccct
     4801 tctcacccac gtcccgctgc tcgggcagca ggtcgatcac gggatgggcg gtgaacatgt
     4861 tgaactcggt actgttcggc tctgttgttc ccagcacgaa acgcgccagc tcaatgcaga
     4921 tgatctgcat ccccagacaa agcccaaagt aggggatgtc gtgct
//
LOCUS       DATALS010000328        10616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_259160, whole genome shotgun sequence.
ACCESSION   DATALS010000328 DATALS010000000
VERSION     DATALS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10616
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259160"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VHS06_07335"
     CDS             <1..526
                     /locus_tag="VHS06_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2987967.1"
                     /translation="FHFPTLWMSFFDFFPVNHRFIDSRLNGRYPLPKDGAPFPVDHPL
                     GAAMMIRREALESVGMLDEEYFMYCEEVDWCIRAKQSGWQIYQLPSAKVVHHVGGSSR
                     QFREEMLIQLHRSRYRLFGKHYGSGFVAAHRAITRLGLAREMSRTRWEACRGRMSKEE
                     MERRLRTYETIWGM"
     gene            528..1298
                     /locus_tag="VHS06_07340"
     CDS             528..1298
                     /locus_tag="VHS06_07340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2987968.1"
                     /translation="MSTVCAAVIARDAATIIERCLRSLQWADSLLVVVDDRTVDATAE
                     VAASVGARVEHHRFEDYARQRNAALEMADADWVFFVDADEEGTPELAEEIRWITGLYE
                     GAAPGYWVPRRNIILGRWIRHAGWSPDYQMRLLRRADAHYREDRIVHELVDLKGQAGY
                     LRNPLTHYNYATLGEFLARQRRYAHMEAINRNRQGVRIKPQNYVLQPWRQFWRRFVTL
                     EGYREGWLGFVLSVLLGYYELRTYLELRKLTSGGGGRP"
     gene            complement(1502..2644)
                     /locus_tag="VHS06_07345"
     CDS             complement(1502..2644)
                     /locus_tag="VHS06_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2987969.1"
                     /translation="MKAVVYHGPHDVKVEDVQQPSIQDPQDVILKVTLATLCGTDLHP
                     YRGSLPGFQDGTILGHEFVGVVDQVGDKVKNLQPGDRVLVPSTIACGYCAYCTEGLYA
                     QCENANPITHSTAYFGGPQEAGGFAGGQAEFVRVPFATVGPVKIPDNVADEQAILLTD
                     ILPTGYFGADLAQIESGESVAVFGAGPVGLMAMESAKLMDAGRIFAIDNIRGRLNVAT
                     QRGFEALDFSSGDPVQQIMDLTGGNGVDVAIDAVGVDAHCAITAQGGNGQDCSTQAIR
                     WALTCVKKAGVVSVIGIYPSTVNGFPLGLILDRNLTLRGGSCNHRAYIPQLLRMVQDG
                     TLDPSFVITDTMPLEKAPEVYAEFDAHQDQHLKVVLETPAAIHIAG"
     gene            2917..3519
                     /locus_tag="VHS06_07350"
     CDS             2917..3519
                     /locus_tag="VHS06_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX2987970.1"
                     /translation="MLSLGRRQEVIEDPPVARALFGDTRLSWLWLLVRLYLSYSWITA
                     SLHKLEDPAWTVTGNALKGYWTNAVGTPDKAGLITFGWYRDFLSMLLNGGHYVWFAKL
                     VAFGEMAIGIGLLIGAFVGVAAFFGGFMNWNFMLAGTTSTNPVLFALSVLLILAWKTA
                     GFWGLDRFLLPMLGTPWRPSSVFTPGAKTAQARASSTPRT"
     gene            3673..5340
                     /gene="hutU"
                     /locus_tag="VHS06_07355"
     CDS             3673..5340
                     /gene="hutU"
                     /locus_tag="VHS06_07355"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX2987971.1"
                     /translation="MATTTSGPRVVRAPRGSQLSCKGWEQEAALRMLMNNLDPEVGER
                     PQDLVVYGGRGKAARSWDAFDAIVRELRQLENDETLLVQSGKPVAVFRTHEDAPRVLI
                     ANSNLVPHWATWEEFDRLEAMGLTMYGQMTAGSWIYIGSQGIVQGTYETLASAADKHF
                     GGTLKGKLVLTAGLGGMGGAQPLAITMNEGVALIAEVDERRIQRRIETGYLDSWTASL
                     DEALARARDAQSAGTPFSIAVLANAADVTTELIRRGITPDIVTDQTPAHDPSMYVPQG
                     MSVAQADELRESDRTRYVELAYKSMAEHTRAMVEFVHRGAIVFDYGNNLRQRGKEAGV
                     EDAFSYPGFVPAFIRPLFCRGKGPFRWAALSGAPEDIFRTDEAMLRLFPDNAGLKRWL
                     ELAHQKIQFQGLPARICWLGYGERDRAGLEFNRMVREGDVKGPIVIGRDHLDAGSVAS
                     PYRETEGMRDGSDAIADWAILNALINTAAGGSWISFHHGGGVGMGFSLHAGMVIVADG
                     SERAERKLARVLKTDPGMGVVRHADAGYPEAIEAARELGVRALSVEK"
     gene            5344..6618
                     /gene="hutI"
                     /locus_tag="VHS06_07360"
     CDS             5344..6618
                     /gene="hutI"
                     /locus_tag="VHS06_07360"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX2987972.1"
                     /translation="MSDKDQPAVLLIEHAAELVTIAGTQGPRKGSAQSDLGLIRDGAL
                     AAGADGRILAVGTTAEVHATVEPAPGAQVIDASGRAVLPGFVDPHTHTVFAGDRADEF
                     ASRLEGADYLEVLASGGGIISTVRATRAAPAEQLEERASRYLEEMLRHGTTTLEIKSG
                     YGLSTEDELKMLRVASALEKTGPQGIVATFLGAHAVPPEYRGQPDRYVDLVVDEMMPR
                     VAAEGLARYCDVFCERGAFSVDQSRRVLEAGLARGLGAKIHAEQKSALGGARLAAELG
                     AVSADHLEYATDEDVAALVEAGTVAVLLPGAALMLMESHWAPARRLIEAGVPVALATD
                     FNPGTCPILSMPLILGLACLRRLVTPAEAVVAATLNAAHAVGLGEQVGSLEPGKRADV
                     VILDAPSHVHLAYWFGRNLVSMVIKDGRVVHS"
     gene            6723..7856
                     /locus_tag="VHS06_07365"
     CDS             6723..7856
                     /locus_tag="VHS06_07365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2987973.1"
                     /translation="MILNGAIGRIGGLLVRHALATTANYLEDNSLQVVDTWIERLRGS
                     MPSYAPLIPEMRQRGESNLNLLVQLLRLEEQTERDATMSELLRIGREMASLHLARGIP
                     LYEIVRATSLFRLSVLNAVERMLRSRVWIAFPHDILKAEDIINVALDNQMLVTSESYL
                     EERDKTIQQSQSELEATNSQLKVLVQEMHHRIKNNLQTIADLLTLEIHSGRKIPTEVA
                     LRESISRVRSIAVVHELLSADDVEVADIKELADVISENCARGMAAAHGGVQVVVQGES
                     ILLPSKQATALALVMTELVNNALEHAFGPEGGTLLVSLDDQADEVRVSVRDNGKGLPD
                     GFNVETSPNLGLQIVRTLVTKDLSGTLELKSNGGTTATVRFRR"
     gene            7856..8434
                     /locus_tag="VHS06_07370"
     CDS             7856..8434
                     /locus_tag="VHS06_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987974.1"
                     /translation="MESLRVVIADDETIRLMSLKAQLESLGLQVVGEAVNGKQALDLV
                     LRLRPDLAILDIKMPEMDGLEAAKAIAGEYPIPVIVLTAYSEHSLAERAIEAGVFAYL
                     MKPVSEEDLLPTLLLAKSRFHEFQDLRKGISDLKEALETRKLIERAKGILMERRNLTE
                     AEAFRRMQIQSQNENKKLSEIAQAIIMADRML"
     gene            8654..9391
                     /locus_tag="VHS06_07375"
     CDS             8654..9391
                     /locus_tag="VHS06_07375"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX2987975.1"
                     /translation="MWNPGNEAGKEDVKPRIPPATVQRLPLYVQALKALAREGEELIS
                     SQRLAMETGIEAAKIRRDFSYLGAFGTPGVGYAVTDLLHEVSHYLGLSRIWPVALIGY
                     GKLGSALAAYAGFAERNFQIVAIFDSNPQRVGLRVGDLKVLHTDQLPKTVGEMGIDIA
                     IIATPAATAQEAADRLIGAGITSILNFAPTTLAAPATVAVRNIDLAAEMQILSFHETM
                     KQNFGLRYPVQPSMERQVSRWLKEQLQ"
     gene            9388..9777
                     /locus_tag="VHS06_07380"
     CDS             9388..9777
                     /locus_tag="VHS06_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987976.1"
                     /translation="MRLGDVARLVEAIPLSHGADMGLEISCACAADLMSDVLAFAGPG
                     ALLLTGLTNPQVIRASDVAGVVAVLFVRGKNPLPETGRLAAETGIVLLQTPHTMFESC
                     GRLYAAGLPEGVATGGMSMDNGREDIA"
     gene            9774..>10616
                     /locus_tag="VHS06_07385"
     CDS             9774..>10616
                     /locus_tag="VHS06_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2987977.1"
                     /translation="MKARYSGNDAATQQEFTKVQELIYELRVDQVMTSPVITLVPQQS
                     MNEAKEMMRFHRISGLPVASNGSIDGILSIEDVIRWLESGGQEAPASDWMTRKVLTVC
                     GDEAAVQAIAKFGRHKVGRLPVVDRAGALIGIVTSGDIIGRALRVLDARYREEEARRG
                     AARCTLDELVGDGTSFALRYEVAPRDFQHAGYAATKVTRILDGLGMDRKIVRRAGIAA
                     YEAEMNLSMHTERGGSLRADIGPGTIVIEALDDGPGIADIEQALTAGYSSAPDWIREL
                     GFGAG"
BASE COUNT         2026 a         3169 c         3450 g         1971 t
ORIGIN      
        1 cttccacttt ccaacactgt ggatgagctt cttcgacttc ttccctgtca accacaggtt
       61 catcgactcg cgactgaacg gcaggtatcc cttgccgaag gacggcgcgc cgtttcccgt
      121 ggatcacccc ctgggcgcgg ccatgatgat ccgccgcgag gcgcttgaga gcgtgggcat
      181 gttggacgag gagtacttca tgtactgcga ggaggtcgac tggtgcatcc gtgccaagca
      241 gtcgggttgg cagatctacc agcttccctc cgcgaaggtc gtccaccacg tcgggggcag
      301 cagcaggcag ttccgcgagg agatgctgat acagcttcac cgcagccggt atcgcctctt
      361 tgggaaacac tacggtagcg gattcgtcgc cgctcacagg gcgataacca ggctggggct
      421 ggcgcgggag atgtcgagga ctcggtggga ggcatgtcgc ggacggatga gcaaagagga
      481 gatggagcgc cggctacgta cctacgaaac catctggggt atgtgatatg tctacggtat
      541 gcgccgctgt aatcgcgagg gatgctgcga ctatcatcga gaggtgcctg cgcagcctcc
      601 agtgggccga cagtctgctg gtggtggtgg acgaccgcac ggtggacgcc acggccgagg
      661 tcgccgcaag cgtcggcgcg cgagtggagc atcaccgctt cgaggactat gcccggcaac
      721 gaaacgcggc gctcgagatg gccgacgccg attgggtctt ctttgtggac gccgacgagg
      781 agggcactcc cgagctggca gaggagattc gatggataac gggtctgtac gagggtgccg
      841 ctccaggcta ctgggtcccg cggcgcaaca tcatcctcgg caggtggatc cgtcacgccg
      901 gctggagccc cgactaccag atgcggctgc tgcggcgggc ggacgcgcac taccgagagg
      961 accgcatcgt ccacgagttg gtcgacctga aggggcaggc cggctacctg cgcaaccctt
     1021 tgactcacta caactacgcg acgctcggag agttcctcgc tcggcagaga cgatacgccc
     1081 acatggaggc aatcaaccgt aaccggcagg gcgtccgtat caagcctcag aattatgtgc
     1141 tgcagccgtg gcggcagttc tggcggcgct tcgtgacgct ggaaggctac agggaggggt
     1201 ggttgggttt cgtcctgtcc gtgcttctgg ggtactatga gcttcggacc tacctggagc
     1261 tacgcaagtt gacttctggc ggaggggggc gaccctgatt tgcctatgcg gcgcgtctgg
     1321 tgtagggcgg gggcctgtcc cccgccgcaa tcgcctggag gcgaccaccg ggcggcaggg
     1381 cacagcgccc tgccctacca gatgacaccc cttcgtttgt ggcggtaggg gcgaggcgta
     1441 ccccgcccct acgttagaga caccgcgcgt gtcccgactt tcccctatgc ggcgacgtgc
     1501 gtcaccccgc aatgtgaatg gctgcaggcg tctccagcac taccttcagg tgctggtcct
     1561 ggtgagcgtc gaactccgcg tatacctcgg gagccttctc cagaggcatc gtgtcggtga
     1621 tgacgaatga cggatccagg gtgccatcct gcaccatgcg cagcagttgc ggtatgtacg
     1681 ccctgtggtt gcaggagccg ccgcgcagcg taaggttcct atccaggatc agccccagcg
     1741 ggaacccgtt caccgtggaa ggatagatcc cgatgacgga tactacgccg gccttcttaa
     1801 cacacgtgag ggcccacctg atcgcttgag tggagcagtc ctggccgttc ccaccctggg
     1861 cagtgatggc gcagtgagcg tccaccccca cagcgtcgat ggcaacgtct accccgtttc
     1921 cgcccgtgag atccatgatc tgctggacgg gatctcccga ggagaagtcc agcgcctcga
     1981 accccctctg cgtcgcgacg ttgagccggc cccttatgtt gtcgatggcg aagatgcgcc
     2041 ctgcgtccat cagcttagcg ctctccattg ccatcaggcc taccgggccg gcaccgaaga
     2101 ccgcgacgga ctctcccgac tcgatctggg cgaggtcggc cccgaaatag cccgtgggca
     2161 ggatgtcggt gagcagtata gcctgctcgt cggcgacgtt gtccgggatc ttcaccggcc
     2221 cgaccgtggc aaatggaacc cgaacgaact cggcctgtcc gcctgcaaag ccgcccgcct
     2281 cctgaggacc accgaagtag gcagtggagt gagtgatcgg attggcgttc tcgcactggg
     2341 cgtacagccc ttccgtgcag taggcacagt agccgcaggc gatggtggag ggaaccagta
     2401 cccggtcgcc gggctgcagg ttcttcacct tgtcgcctac ctgatccacg actccgacga
     2461 actcatgtcc caggatggtg ccatcctgga agccggggag actgcccctg taagggtgaa
     2521 ggtcggtgcc gcacagcgtg gccagggtga ccttcaggat cacgtcttga gggtcctgga
     2581 tggagggctg ctgcacgtcc tccactttta catcgtgcgg cccatgatag accactgctt
     2641 tcatagcctt tgaactcctc gtcgacagta tcgttctggc ctccacctcg acgtgaaggg
     2701 tgcggcaacg aagcaacctt tgggccacgc ctgctccgtg gagcggcgac atatctagaa
     2761 accgcccgcg gcgtacccat agcaggattg ccatcattca ggcgataaaa agctcttgac
     2821 acagttaatg cggctaagta tactcgtcgt gaactcgaag ccaagctgtt ggtgttcgag
     2881 agcactacat cccccgtggg ggaaaggaga ccataaatgt tgtctcttgg acgaaggcag
     2941 gaggtcatcg aggatccgcc ggtcgcgcga gcactattcg gcgataccag gctctcctgg
     3001 ttatggctgc tggttcggct ctatctgagc tactcctgga tcaccgccag cctgcacaag
     3061 cttgaggatc ccgcctggac ggtcacaggc aacgccctca agggctactg gactaatgca
     3121 gtgggaaccc cggataaggc cggcctcatc actttcggtt ggtatcgaga cttcctctcc
     3181 atgctcctta atggcggcca ctacgtttgg tttgcgaagt tggttgcctt cggcgagatg
     3241 gcgatcggca ttggcctgct gatcggtgcg tttgtcggcg tcgccgcctt cttcggcggc
     3301 ttcatgaact ggaacttcat gctggctggc acaacaagta ccaaccccgt actgtttgcc
     3361 ctgtcggtcc tgctgatcct cgcctggaag acggcgggct tctggggcct agaccgcttc
     3421 ctgctcccga tgctgggtac cccctggcgc cccagcagtg tcttcacgcc cggggcgaag
     3481 acggcccagg cgcgagcgtc atcgacgccg agaacgtagc gagataccct ctctggacac
     3541 actgaggggc cggattcgtc cggccccttt tctcattggt gcgccttcac caagctattg
     3601 acgccgcctt cgactctccc tacagttgag aggatgatag tcgctgatgt ggaactccaa
     3661 ggagggacag ctatggcaac gaccacgtct ggaccgcgag tggtgcgagc cccccgcggc
     3721 agtcaactca gctgcaaagg gtgggagcaa gaggcagctc tccgaatgtt gatgaacaac
     3781 ctggatccag aggtgggcga gcggccacag gatctggttg tatacggcgg tagagggaag
     3841 gctgcccggt cctgggacgc cttcgacgcg atcgttcgcg agcttcggca actggagaac
     3901 gacgagacgc tgttggtgca gagcgggaag ccggtcgcgg tgtttcgcac tcacgaggat
     3961 gcgcctcggg tgctgatcgc caactccaac ctcgttcccc attgggctac ctgggaggag
     4021 ttcgaccgcc tggaggccat gggcctcacc atgtacggcc agatgactgc cggcagctgg
     4081 atctacatcg gaagtcaggg gatcgtccag ggcacttacg agaccctggc ctctgccgcc
     4141 gataagcact tcggaggcac gctgaaaggc aagttggtgc tgacggcagg ccttggcggg
     4201 atggggggcg cccagcccct ggccataacc atgaacgaag gggttgcgct gatcgctgag
     4261 gtggacgagc gcagaattca gcgccgaatc gagactggat acctggatag ctggacggcc
     4321 agcctggacg aggcgctggc cagggcgcga gatgcccaga gtgcgggcac tcccttctcc
     4381 atcgcggtac tggccaacgc cgcggatgtc accacagagt tgatccggcg tgggattacg
     4441 ccggacatcg tcaccgacca gacccctgcc cacgaccctt cgatgtatgt gccgcagggg
     4501 atgagtgtgg cccaggcaga cgaactgcga gagtctgacc gcacgcgtta tgtggagttg
     4561 gcctacaagt cgatggcgga gcacaccagg gcgatggtgg agttcgttca ccgtggcgcg
     4621 atcgtcttcg attacggcaa caatctccgc cagcgaggca aggaggccgg cgtcgaggat
     4681 gcattcagct atcccgggtt tgttcccgcc ttcattcgcc ccctattctg ccggggcaag
     4741 ggccccttcc gatgggcggc tctatccggc gcacccgagg atatcttccg caccgacgag
     4801 gccatgctgc ggctcttccc cgacaacgct ggtctgaagc gttggctgga actggcccat
     4861 cagaagatcc aattccaggg actccctgcg aggatctgct ggttgggcta cggcgaaagg
     4921 gatagggcag gtctggagtt caaccgtatg gttcgggaag gcgacgtgaa gggtcccatc
     4981 gtcatcggtc gggaccacct ggatgccggt tcggtggcct caccttacag ggaaaccgaa
     5041 gggatgcggg atggatccga tgccatagcc gactgggcga tactgaacgc cctcatcaac
     5101 acggcggccg ggggaagttg gatcagcttc catcatggcg gcggcgtggg aatgggtttc
     5161 agcctgcacg ccggaatggt gatcgtggcc gacggcagcg agcgggcaga gcgcaagctg
     5221 gcccgagtgc tgaagaccga tcccggtatg ggagtggtgc gccacgctga cgccggttat
     5281 ccggaggcga tcgaggctgc cagggagttg ggcgttcgtg cgctgagtgt ggagaaatag
     5341 gttgtgtccg ataaggatca gcccgcagtc ctgctaatcg aacatgccgc cgagttggtc
     5401 accatcgcgg ggacgcaagg gccgcgaaag gggagcgctc agagcgatct cggcctgata
     5461 cgggatggag ccttggcggc aggagccgat ggacgcatcc tggccgtggg tacgacggca
     5521 gaggtgcacg cgacggtcga gccagcgccc ggggcccagg tcatcgacgc ctcgggccgg
     5581 gctgtgctgc cgggtttcgt ggatccccac acccacacgg tcttcgccgg cgaccgcgcc
     5641 gacgagttcg cgtccagact ggagggcgcc gactacctcg aggtcctggc gagcggtggt
     5701 gggatcatta gcactgttcg ggcaacccga gcggcccccg cggagcagtt ggaggagcgg
     5761 gcgtccaggt accttgagga gatgcttcgc cacggcacta ccacgctaga gataaagagc
     5821 ggctacggcc tctccacgga ggacgagctg aagatgctgc gggtggcctc ggcgcttgag
     5881 aagacagggc cgcaggggat cgtggcgaca tttctgggag cccacgcagt gcccccagag
     5941 tacaggggac aacccgaccg ttacgtcgac ctggtggtcg atgaaatgat gccccgggtt
     6001 gccgccgagg gcctggcccg ctactgcgat gtcttctgcg agcgaggggc cttctcggtc
     6061 gaccagagca ggcgggtcct ggaggccgga ctggcccgcg gattgggcgc gaagatccat
     6121 gccgaacaga aatccgccct gggcggtgct cgcctggctg ccgagttggg tgccgtgtcg
     6181 gcagaccacc tggagtacgc caccgacgag gacgtcgccg ctctagtcga ggcggggact
     6241 gtcgccgtcc tgttgccggg ggcggccttg atgctgatgg agagccattg ggctcccgca
     6301 cggcgattga tcgaagccgg agttcccgtg gcgttggcga ccgattttaa cccggggacc
     6361 tgcccgatac tctccatgcc gctgatcctg gggctggcct gtcttcgccg cttggtcaca
     6421 cctgccgagg ccgtggtcgc ggcgacgctg aacgccgccc acgctgtggg gctgggggag
     6481 caggtcggaa gcctggagcc cggcaagcgg gcagacgtcg tgatcctgga cgctccttcc
     6541 cacgtgcatc tcgcctactg gtttggccgc aacctcgtaa gcatggttat caaagacggg
     6601 cgtgttgtgc actcgtgact ctcaacttct gcctaactcc tctccacttg ccttgaccca
     6661 cctcagccca gtctgtaaac tctgaaatgc tgcgaatcgg ccattcgagt ggggcacgtg
     6721 ctttgatctt gaacggtgcc atcggtcgca tcggcggact cctcgtccgg cacgctcttg
     6781 ccacgaccgc caactatctg gaagacaact ctctgcaagt agtcgacacc tggatcgagc
     6841 gcctccgagg atcgatgccg agctacgctc ccctgatccc ggaaatgcgg cagcggggcg
     6901 agagtaacct gaacctgctg gtgcagcttc ttcggctgga ggagcaaaca gaacgtgacg
     6961 ccacgatgtc ggagttgctc cgcatcggca gggagatggc ctccctccat ctggcacgtg
     7021 gcatcccctt atacgagatc gtgcgcgcga cgtccttgtt caggctctca gtgctgaacg
     7081 ctgtggagcg tatgcttcgc agccgggtgt ggatcgcttt cccgcatgac atcctcaagg
     7141 cagaggatat catcaacgtt gccctggaca accagatgct ggtcacctcc gagtcctacc
     7201 tggaggagag ggacaagact atccagcaaa gccagtcgga gctagaggcg accaatagtc
     7261 agttgaaggt gctggtgcag gagatgcacc atcgcatcaa gaacaatctg cagacgatag
     7321 ctgacctgct caccctggaa attcacagcg gccggaagat ccccaccgaa gtggccctca
     7381 gggagagcat cagccgtgtt cgtagtatcg ccgtggtgca cgagttgctt tcggcggatg
     7441 acgtcgaggt cgccgacatc aaggaactgg cagacgtgat ctccgagaac tgtgccaggg
     7501 gcatggcggc cgcacacggt ggagtgcagg tcgtggtgca gggtgaatcg atcctcctcc
     7561 catccaagca ggcaacggca ctggcactgg taatgaccga gctagtcaac aacgcactgg
     7621 agcatgcctt cgggccggaa gggggcacct tgctggtgtc tctggacgat caggctgatg
     7681 aggttcgggt ttcggtgcgc gacaacggca aagggctacc cgacggtttc aatgtggaaa
     7741 ccagtcccaa cctggggctg cagatagtcc gcacgctggt aacgaaggac ttgagcggca
     7801 ctctggaact gaagtctaac ggcggaacga ccgccactgt gaggttcagg agatgatgga
     7861 gtcgctgagg gtcgtcatcg cagacgacga gacaatccgg ctaatgagtc tgaaagccca
     7921 gttggaatcc ctcggtctgc aggtggtagg agaggccgtc aacggcaaac aggccctcga
     7981 tcttgtcctt cgactgcgac ccgaccttgc catcctagac atcaagatgc cggagatgga
     8041 tgggctggag gcggccaagg cgatcgccgg ggagtatccc attcctgtca tcgtattgac
     8101 tgcctacagt gagcactccc ttgcagagcg ggcgatcgag gccggtgtgt tcgcctacct
     8161 catgaagccg gtctctgagg aggacctcct ccccaccctc ctccttgcga agtcccgctt
     8221 ccacgagttc caggatctgc ggaaggggat cagcgacctg aaggaagcac tggagacccg
     8281 caagttgatc gagcgagcca aggggatcct catggagcgg cggaacctca ctgaagccga
     8341 ggcgttccga cggatgcaga tccagagtca gaacgagaac aagaagctct ctgagatagc
     8401 ccaggccatc atcatggccg atagaatgtt ataggaagcc gccccacttc agaacatcct
     8461 tcaactagac tgttgacact gtcgtgcccc tgtgtataat ggattgtgaa ccgtttcacc
     8521 aatgctggtg aattcccata tcctgagctg acgatgggct ccggcaccgc acggcgagct
     8581 tgcacggcgg ggccggggcc tctttgttcg cttcatattg tgaggtgttt cacagagaaa
     8641 gaagaggctc gggatgtgga atcctggcaa cgaggcagga aaagaagacg tgaagcccag
     8701 aattcccccc gcaaccgtgc agcgtctgcc cctctacgtc caggccctca aggcgctggc
     8761 gagagagggt gaggagttga tctcctctca gagactcgcg atggagacag ggatcgaggc
     8821 cgcgaagatc cgccgcgact tctcctacct gggagccttc gggaccccgg gtgtcgggta
     8881 tgccgttacc gacctgcttc acgaggtctc acactacctc ggcctctctc gcatctggcc
     8941 tgtggccctc ataggttatg gaaagttggg gtcggccctc gccgcctacg ccggctttgc
     9001 ggagcgcaac ttccaaatcg tcgccatctt cgactccaac ccgcaacgcg tgggtctgcg
     9061 ggttggtgac ctgaaggtgc ttcacaccga ccagttgccg aaaaccgtag gcgaaatggg
     9121 gatcgacatc gccatcatcg ccacgccggc cgccaccgcc caggaagccg ccgatcgact
     9181 gatcggcgcg ggcatcacct cgatcctgaa cttcgccccc actacgctcg cagcacccgc
     9241 taccgtagcc gtgcgcaaca tagacctggc ggcggagatg cagatcctct ccttccacga
     9301 gacgatgaag cagaacttcg gtctccgcta ccccgtccaa ccgtccatgg aacggcaggt
     9361 ttcccgttgg ctcaaggagc aactccaatg agactcggcg atgtggcaag gttggtcgaa
     9421 gccattcctc tgtcccatgg cgcggacatg ggactggaga taagttgtgc ctgtgctgcc
     9481 gacctcatga gcgacgtgct cgcctttgcc ggccccggcg cgctgctgct gacgggtctc
     9541 accaacccac aggtgatacg ggcttcagac gttgccgggg tagtggcggt gctgttcgtc
     9601 aggggcaaga acccgctgcc tgagaccggg cggctggccg ccgagaccgg gatagtctta
     9661 ctgcagaccc cgcataccat gttcgagagt tgtgggaggc tgtacgctgc cggcttgccg
     9721 gagggggtgg ccaccggcgg tatgtcgatg gacaacggca gggaggatat agcgtgaagg
     9781 ccagatatag cggcaacgac gccgccacgc agcaggagtt caccaaggtc caggagctga
     9841 tttacgagct gcgggtcgat caggtgatga cctcgcccgt gatcaccctc gttccgcagc
     9901 agtcgatgaa cgaagccaag gagatgatgc gctttcacag gatctcagga ctccctgttg
     9961 cctcgaacgg ctccatcgac gggatcctca gcatcgagga tgtcatacgg tggcttgaga
    10021 gtggcggcca ggaggctcct gcgtcggact ggatgaccag gaaggtgctg acggtctgcg
    10081 gtgacgaggc ggccgtccag gcgattgcca agttcggccg gcacaaggtt ggcaggcttc
    10141 ctgtggtcga ccgagcgggg gcactaatcg gcatagtgac gtcgggcgac atcatcggaa
    10201 gggcacttcg ggtgctggac gcccgatatc gcgaggagga agcacggagg ggcgccgcac
    10261 gctgcaccct ggacgaactg gtgggggacg gcacctcttt cgcactccgc tacgaggtcg
    10321 ccccgaggga ttttcagcac gcgggctacg ccgccaccaa ggtgacgcga atcctggatg
    10381 gactggggat ggaccgcaag atcgtgagaa gggcaggcat cgccgcctac gaggcggaaa
    10441 tgaacctgtc gatgcacaca gaaagaggag gatcccttag ggccgacatt gggccgggga
    10501 caatagtcat cgaggccctg gatgacggcc ctgggatcgc tgacatcgag caggccctca
    10561 cggccggcta ctcgtctgct cctgactgga tccgagagct gggcttcggt gcgggg
//
LOCUS       DATALS010000329        10092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_286457, whole genome shotgun sequence.
ACCESSION   DATALS010000329 DATALS010000000
VERSION     DATALS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286457"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VHS06_07390"
     CDS             complement(<1..526)
                     /locus_tag="VHS06_07390"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEX2987978.1"
                     /translation="MTWQITPISVITMLAAVAAGATAIETWRHRSARGGLQLALLLVA
                     ITEWCASVTLESAAVGIPAKVFWSKAEYVGLTCSITLLLQFALVYSQQDRWLARWRIH
                     LLWAIPATFILLAWTNELHGLLWPSLVMAPGEGNVLIYGHGPVFFVGGAFYTLVMLLS
                     AALLLIPMHNMPRLY"
     gene            complement(793..1137)
                     /locus_tag="VHS06_07395"
     CDS             complement(793..1137)
                     /locus_tag="VHS06_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018803106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2987979.1"
                     /translation="MGIFSWLFGPEVETIEVTDVQRLQAEGAVVIDVREPHEWAMGHV
                     QGAKLMPLGSLNRELSKIRPDRQVLFICQSGHRGARATDMANRRGLKAVNVRGGMMRW
                     MRAGLPVKNGRK"
     gene            complement(1321..1965)
                     /locus_tag="VHS06_07400"
     CDS             complement(1321..1965)
                     /locus_tag="VHS06_07400"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX2987980.1"
                     /translation="MQRGLPAASPSQILYRIGRLPNPLAWPPRGLTGAGRFDDPLGQF
                     STLYAAEQRRACFIESLARFRASPKVLAELRRVSGTSDTVLSPTVPLDWCAKRGVARF
                     YLRAGQRGLDLRSIETREALRAELAELLVSLDLADLDVGDVLSPNRQLTQAISRWAYE
                     RGYNGIAYKSRLDDDLDCWALFQGATIEMVGSIEPISPHDPDFRETATLFGLVI"
     gene            1960..2244
                     /locus_tag="VHS06_07405"
     CDS             1960..2244
                     /locus_tag="VHS06_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987981.1"
                     /translation="MHYLQDHDADLLILDMVMPDGIDGTETYHRACEIRPGQRAVIVS
                     GFAETELVRQAQAMGAGSYIRKPVTLEKLARAVRQELDRPTRETQTAKKG"
     gene            2897..4042
                     /locus_tag="VHS06_07410"
     CDS             2897..4042
                     /locus_tag="VHS06_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX2987982.1"
                     /translation="MCEGTATADQWPRLVQVAHLQEMVDRLRRERGVPVELVDLNGVS
                     KDEALLVKLGERSMLVPLEGRTLFDLHDQPDSYSKDVGRGSYYIAPLPLRADVVVSLA
                     KMKVHRAAGVTMAMKNLFGIIPSWDGPYGDNRLKDVPHYSDREAAGGPRTLYLDNDTT
                     WRTIVDLNQILLFADDRAQMHTDRQRRYLAIVDGLTAAGTDMFDPKAVPLGLLVVGDT
                     PVGADAVAARLMGYDPRLIKSIAWAWSLGVTPLGPASPSLIDVKVAGALTANEVADGH
                     MVVPPSAAPYPWHGHLEAGDFQPPEIDAPQLQGNELQVVLKDGSGVAFARIVQTAGGQ
                     NVESELGLVGGTASEGEWRGNLPSGSRDTMVLEVGDSLFNVRRRPLK"
     gene            4111..5703
                     /locus_tag="VHS06_07415"
     CDS             4111..5703
                     /locus_tag="VHS06_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2987983.1"
                     /translation="MNRRPRGRSLLYGALLGGLTSLPVIALFYLGKQVVGAPFVPYDF
                     FEWISRVLPGGVVAGAIDSMVAVIQALHLGQIDQAAKLIEQLMAIAINVAIGALFGIL
                     IVWGRDATPWSASSIGFFGSMALFLMAIVMEVSLGNVSNPELTLLWTTLLIVGWGAAL
                     GYILDAVIAQEGAGRLAPEYQPERRRALMNILGGSFALALAGSGLGWLLQEGRRDSGA
                     GVPLKGPVPPAPTIEPNRIEPAPGTRSEITPNRDFYRIDIDLRPPVVDGNSWMLAVDG
                     LFERSRNLTLSDLMAYPAVTQPITLSCISNPIGGELIGTGYWTGVRLATLLQDLGLRP
                     EARALRIEATDGFYESVVMANVDDPRTLLVYGMNGETLPVEHGFPLRIYIPNRYGMKQ
                     PKWITRMQAMEQEQDGYWVERGWSKEARPQIVSVIDTVAANSAVDGKIPIGGIAWAGD
                     RGIQQVELQVDGGEWQPATLRVPPVGPLTWVQWRYDWPGVAGRHTFRVRATDGTGALQ
                     IEKEMGTFPDGATGYHSVAKDI"
     gene            5866..7698
                     /locus_tag="VHS06_07420"
     CDS             5866..7698
                     /locus_tag="VHS06_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX2987984.1"
                     /translation="MPSTLGRAVAALALFVLVTLEMAMPAGSYAAGCSFVGGFAALHD
                     LIPDTVGPCTADERHAPYSGDTIQPTAGGTLIWRQADNVTAFTNGYHTWINGPQGFQQ
                     RETGERFSWETPETGWVLDHPRDQNLTPEQRTSRYFDSIRGKPTQLAQFIARMPKGAD
                     LHNHLSGAVYAESYVRYAIQDGLCVDQKSFTLTKPPCAPSAGIWPASDALDDRELYDA
                     MVNAWSMRDFQPTSGQSGHDHFFDTMGKFNAATETHAGEMLAEAVSRAAADNVQYVEY
                     MLTPDPGTASALGEQVGWDSDMAATREKLLQAGLADITADARQRLDQMETTMRATLRC
                     DTARAESGCSVRVRYVAQTNRTGTPASVFGQLVANFELAKVDPRLVGVNLVAPEDDRV
                     GLRDYSLHMSMLEFLHDLDPQVNITLHAGELAPGIAPSDALSSHIWEAVVKAGARRIG
                     HGADLMLEHDRYDLLDEMARRKVPVEINLTSSELILELSGLSHPLPMYMRFGVPVTLS
                     TDNQGVSRGGMNKEYTRAASTYGLWYDDLKRLARNGIEYSFLPGASLWERADVFIPVA
                     ACSKERPSTTEPSSAGCKAFLDTSEKARQQWRLEASYAAFENGY"
     gene            7796..9181
                     /locus_tag="VHS06_07425"
     CDS             7796..9181
                     /locus_tag="VHS06_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX2987985.1"
                     /translation="MQLIGIDLGTSFLKGAVLDLDPPSIGHIHREPVPNPVPGLPPLW
                     NEYDAGEILAATRRLLIELAPFAPDCIGLMVSNQMSSLVLSDGEGRALSNCIGWRDQR
                     SLASHPSGAGSYFDRLAESITPEERIQMGNELRPGLPISFLAWMAEAGCLPSGAIPAS
                     LGDFVAASLCNTAPSSDPTNGSAHGTLNIETVDWHYPVLERLGLGSLRWPQLHRSGDV
                     VGTVRIGAREVPCYTPMGDQQVALASTFLKPRELWLNISTGSQVSLLTPTPQFGAAYR
                     TRPSADGRFYKTITHIPGGRSMDQIMRLLCELAQAEGLQLRDPWGYAAHQAELLEQTD
                     LKVNLSFFLSSCGDRGEISNIGEENLTVGHIFRAAYQNMADNYADCALRLSPDREWDS
                     LVFSGGLAQKMGQLRRLIAEKFQTGYRMTASSEDALLGLLALGLLFTGQARTLEEAGR
                     LIPEEHIQESR"
     gene            9181..9831
                     /locus_tag="VHS06_07430"
     CDS             9181..9831
                     /locus_tag="VHS06_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX2987986.1"
                     /translation="MYKALVFDLDGTLVDTPAGIARTIARVLANRGYPVPSSEEVYQM
                     IGLPLVDSFLRFLPADARSIAPECVVEYRDLYERIEIPATTLFPGVGETLARFQAAGV
                     RMAVATAKLTRVAESTLAHSGARGYFDAVLGGDCVSEPKPHPQLALSALARLECSPES
                     ALVVGDTSYDIQMGKAAGTATCGVTYGVQPRTQLAGASPDYLIDRFAELAAILGTE"
     gene            complement(9833..>10092)
                     /locus_tag="VHS06_07435"
     CDS             complement(9833..>10092)
                     /locus_tag="VHS06_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX2987987.1"
                     /translation="VIFAAGALITTTVAVGTLWIGYKLMKIPMGLLTGMLAGLQTQPA
                     VLGFSLEQTRNELPNIGYAMVYPMAMIAKILLAQLLLSLLM"
BASE COUNT         1909 a         3258 c         3178 g         1747 t
ORIGIN      
        1 ggtagagtcg tggcatattg tgcattggga tgagcagaag ggcggccgaa aggagcatta
       61 cgagggtgta gaatgcgcca cctacgaaaa agaccgggcc gtggccgtag atcaggacat
      121 tgccttcccc gggggccatg accaggctgg gccacagcag accgtgcagc tcgttggtcc
      181 aggccaggag gatgaaggtg gcgggtatcg cccacagcag gtggatgcgc catcgcgcga
      241 gccacctgtc ctgttggctg tagacgaggg cgaactgcag caggagggtt atcgagcagg
      301 ttaggccgac gtactccgcc tttgaccaga agactttagc cggaattcct acggcggctg
      361 attccagggt gacggaggcg caccactccg tgatggcgac cagcagcagc gccagctgaa
      421 gcccgccccg cgccgacctg tgtcgccagg tctcgatcgc ggtagcgcct gccgcgacgg
      481 cggcgagcat cgtgatgacg gaaatgggag tgatctgcca ggtcatgatt caattacccc
      541 tatctaccga gcactatcgg gcatggaagt ctgacccgaa gacactgctg tgtgatttcc
      601 accattattg cacgccggcg tgtcacgcag cgtgtcaccg cggtaaacgg agaattcttg
      661 aggatggccg ctgttcggag ggcaaaattg acccaataga ttcatcggca ggcgatgggg
      721 aatctgaagg tttagatatg tgggtttcgc cctcttctgt gggcggcggt catttctcga
      781 gggtgagtct ccctatttgc gaccgttctt caccggcagc cccgctcgca tccaccgcat
      841 catcccaccg cgcacgttca cggccttgag cccccggcgg tttgccatgt cggtggccct
      901 tgcgccccgg tgtccggact gacagatgaa caggacctgc cgatcgggcc gaatcttcga
      961 aagctcgcgg ttcaggctgc ccaggggcat cagcttcgcc ccctgcacgt ggcccatcgc
     1021 ccactcgtgg ggctctcgca cgtcgatcac gaccgcgccc tcggcctgaa gccgctggac
     1081 gtccgtcact tcgattgtct caacctcggg cccgaacagc caactgaaga tgcccatcta
     1141 tatcgagtcc cccaatagaa agccgtagag tataccccta tagggtatac tctacggcgg
     1201 caatgttcaa ggccaactgg aacgtttgct cggatgtcag ggttccatcg atgagcggac
     1261 gggtgcagcc cacgttgtgt ctcggaggat gatccgctta ggagtggagg ggcggttgcc
     1321 ctagatcacc aggccaaaca gcgtggcggt ttcccggaag tcgggatcgt gaggagagat
     1381 tggctctatg gagccgacca tttcgatggt ggcaccctgg aagagcgccc aacagtccag
     1441 gtcgtcgtcc agccggctct tgtaggcaat cccgttgtag ccgcgttcgt aggcccaccg
     1501 ggatatggcc tgggtcagtt ggcggttggg gctgagcaca tcccctacgt ccaggtcggc
     1561 gaggtcaagg ctcacgagca actctgctaa ttccgcgcgc agcgcctcgc gcgtttcgat
     1621 gctacgtaga tctaacccgc gctgcccggc gcgcaggtag aaccgagcga cgccgcgttt
     1681 ggcacaccaa tcgagaggca cggtgggtga gagcaccgtg tcggatgttc cggatactct
     1741 cctcagttct gccagaacct tggggcttgc cctgaacctt gccagcgact cgatgaagca
     1801 ggcacggcgc tgttcggcag cgtagagggt gctgaactgt ccaagggggt cgtcgaatcg
     1861 acccgcccct gtaagtcccc gtggcggcca ggcgagaggg ttgggcagcc ggcctatccg
     1921 atagaggatc tgcgacggcg aagccgcggg gagtccgcgt tgcactacct ccaggatcac
     1981 gatgcggacc tcctcatcct ggacatggtg atgccggatg ggatcgacgg cacggagacc
     2041 taccaccgcg catgcgagat aaggcctggg caacgggcgg tgatcgtctc cggcttcgcc
     2101 gaaacggagc tggtgagaca ggcccaggcc atgggcgccg gaagctatat ccgcaagccg
     2161 gtcacactgg agaagctcgc acgagcggtg cgccaggaac tcgatcgccc cacccgcgag
     2221 actcagacgg ccaaaaaggg ttagccgagg cggtccactg gccccatttc tgcgaggttg
     2281 atccctgtag taggtgtgga tctggccagc ccgaggcaac gctgaaaagg aacccatccc
     2341 atggctaacc ggcaaggccg gccgaggcga ctgtcgcggc gtgcgatgct gggactactc
     2401 gcaggaaccg cgctggggtt gggcgccgag gcgctggttg gcccggggct gaccccgcgg
     2461 gtactggaac cctggatcaa ccagctaccg atcctgcccc gcacccctca gccatcacct
     2521 ccccccccag cgtgccaggg tcggcgtcgg ctacataccc aggccggccg gctacaactt
     2581 caccccgccc ttcgatcccg ccgagactta ccccgagtac ccttacaacc agaagccgtc
     2641 cggagagagg aacgacggct acttcctgct gagggaggct ctccggggat ggcagcccga
     2701 ggggttcggc acctcgggct ggaatcccct ggcagccacg atccggccag gcgataccgt
     2761 cgccatcaag gcaaacctgg tcttcgaatc caactgggac caggataggc tcgggctcgt
     2821 caccgttcac ccctccacca tccgggccgt agtcgactac gtcttcaagg catgcgggcc
     2881 cagggggagg attctggtgt gcgagggcac ggccacggca gaccagtggc cccgtcttgt
     2941 ccaagtcgcc cacctccagg agatggtgga tcgccttcgg cgagagcgcg gggttcccgt
     3001 ggaactggtg gatctaaacg gggtctccaa agacgaagcg ctgctggtga agctgggcga
     3061 gcgctccatg ctggttcccc tggaagggag gaccctgttc gacctgcacg accagcccga
     3121 ctcctacagc aaagacgttg gccgcggcag ctactacatc gcccccctcc cgctgagggc
     3181 cgacgtggtc gtcagcctcg ccaagatgaa ggtgcatcga gccgcggggg ttaccatggc
     3241 gatgaagaac ctcttcggga tcatcccttc ctgggatggc ccctacgggg acaatcggct
     3301 gaaggatgtg ccgcactaca gcgatcgcga ggcagctgga ggtccacgaa ccctctacct
     3361 ggataacgac accacctggc gcacaatcgt cgacctgaac cagatcctgc tctttgccga
     3421 cgacagggca cagatgcaca ctgatcgaca gcgacgatac ctggccatag tggacggtct
     3481 cacggctgcc ggaacggata tgttcgaccc caaggctgtc cctctggggc ttctggtcgt
     3541 cggggatacc ccggtcggcg ccgacgcagt ggcggccagg ttgatgggct acgatccgcg
     3601 gctgattaaa tccatcgcct gggcgtggtc gctgggggtc accccccttg gtcccgcttc
     3661 gccatccttg attgacgtca aggtggccgg agcgctcact gcgaacgaag tcgccgacgg
     3721 tcatatggtg gtcccgccct ccgccgcccc ctacccctgg cacggccacc tggaggccgg
     3781 cgatttccaa ccacccgaga tcgacgctcc gcagctgcag ggcaacgagt tgcaggtggt
     3841 gttgaaggac ggcagtgggg tcgccttcgc gcgcatagtt cagaccgccg gaggacaaaa
     3901 cgtagagagc gagctcggcc tcgtgggagg gaccgcctcg gaaggggagt ggaggggcaa
     3961 cctgccctcc ggcagccggg ataccatggt gctcgaggtc ggggacagcc tgttcaacgt
     4021 ccgtcgccgc cctctgaagt agctatgtag tggcatgcct tctgcttcca ccaggatgac
     4081 cagatctgca tcggaactcg ggtggggaag atgaatcgcc ggccgcgcgg tcgatcgcta
     4141 ctatacgggg ccctcctggg agggctgacc agcctgccgg tcatcgcgct cttctacctc
     4201 ggcaagcagg tcgtcggtgc ccccttcgtc ccctatgact tcttcgagtg gatctcccga
     4261 gttctgcccg gcggcgtggt agccggggcc atcgactcca tggtggcggt tatccaggcg
     4321 ctgcacctcg gccagatcga ccaggcggca aagctcatcg aacaactgat ggccatcgct
     4381 atcaacgtcg ccataggtgc gctgttcgga atccttatcg tctggggcag ggatgccacc
     4441 ccctggtcgg cctcctccat cggcttcttc ggcagcatgg ccctctttct catggcgatc
     4501 gtaatggagg taagcctggg caatgtgagc aacccggagc ttacactgct ctggaccacg
     4561 ctgctgatcg tgggctgggg ggcagcgttg ggctacatac tggatgccgt gattgcccaa
     4621 gagggcgccg gaagactcgc tccagagtat cagccggagc gaaggagggc gctgatgaat
     4681 atcctggggg gatcgtttgc cctggctctc gccggttccg ggctcggctg gctcctccag
     4741 gaaggtcgcc gcgactccgg cgcgggggtg cccctcaaag gacctgtccc gcccgcaccc
     4801 accatcgagc ccaacaggat cgagccggcg cccggcaccc ggtctgagat aacccccaac
     4861 cgcgacttct accgtatcga catcgacctg cgcccaccgg tcgtcgacgg caactcctgg
     4921 atgcttgcgg tggatgggct cttcgagcga tcgcgcaatc tcaccctctc cgatctcatg
     4981 gcataccctg ccgtcaccca gccgatcacc ctctcctgca tctccaatcc gatcgggggg
     5041 gagcttatcg gcaccggcta ctggaccggc gtccggctgg ccacgctatt gcaggatctt
     5101 gggctgcgcc cggaggcgag ggcgctgcgc atagaggcga cagacggctt ctacgagagc
     5161 gtggttatgg ccaacgtcga cgacccacgc accctcctgg tctacgggat gaacggggag
     5221 acactccccg tggaacacgg cttcccgctg cgcatctaca tcccaaaccg gtacgggatg
     5281 aagcagccca agtggatcac cagaatgcag gcaatggaac aggagcagga cggctactgg
     5341 gtggagcgag gctggagtaa ggaggcccgc ccccagatcg tatcggtgat cgacaccgta
     5401 gccgccaaca gcgccgtgga tggcaagatc ccaatcggcg gcatagcctg ggcaggggat
     5461 cgcgggatcc agcaggtcga gttgcaggtg gacggcggcg agtggcagcc ggctacactt
     5521 cgggttccgc cggtcggacc gctcacctgg gtccagtggc gatacgattg gcctggggtc
     5581 gccggccgcc acaccttccg ggtccgagcc accgacggca ccggcgcgct gcagatcgag
     5641 aaggagatgg gcactttccc ggacggcgcc accggctacc actccgtggc gaaggacatc
     5701 tgaccaacgc cacaccctcc gaccgcggaa cacggttgtc caacggcacg cggctgaaca
     5761 tccaccatga accggctgga ttgctgtaag ataggggcac acccggcaat cccgaagaga
     5821 aatccgcccc gtctccgcca ccatccgctc aggaggtcgt gtgcaatgcc gtctaccctc
     5881 ggtcgggccg tggcagcgct ggcgctgttc gtcctcgtca cgttggaaat ggccatgccg
     5941 gcgggcagct atgctgcggg ctgcagcttc gtcggtgggt tcgccgccct tcacgacctg
     6001 atcccggata cagtggggcc ctgcacggcc gacgagcgcc acgcccccta tagcggggac
     6061 actatccaac cgacggcggg cggcaccctt atctggagac aggcagacaa cgttaccgcc
     6121 ttcaccaacg gttatcacac ctggatcaac ggtccgcagg gttttcaaca gcgggagacc
     6181 ggggaacggt tctcctggga aacgccggag accggctggg tgctggacca cccccgagat
     6241 cagaacctca ccccagagca gcggacatcc aggtacttcg actccatccg aggtaagccc
     6301 acccagttgg cacagttcat cgcaagaatg ccgaagggag ccgacctgca caaccatctg
     6361 agcggcgcgg tctacgcgga gagctacgtc aggtacgcga tccaggacgg cctgtgcgtg
     6421 gatcagaaga gcttcacact cacgaaacca ccctgcgccc cctctgctgg aatatggccc
     6481 gccagcgatg cccttgacga ccgagagctg tacgatgcca tggtgaacgc gtggtccatg
     6541 cgggatttcc aacctaccag cggccagagc ggccacgacc atttcttcga cacgatgggt
     6601 aagttcaacg ccgccaccga gacccatgcg ggggaaatgc tggccgaagc cgtctcccgc
     6661 gccgctgccg acaacgtaca gtacgtggag tacatgctca cgccggatcc gggcaccgcg
     6721 tccgccctgg gggagcaggt cggatgggat agcgacatgg ccgcgacgcg agagaagctg
     6781 ctgcaggcag ggttggccga catcaccgca gacgcgcggc aacgactgga ccagatggaa
     6841 acaactatgc gcgccaccct tcgctgcgac acggccaggg cggaatccgg ctgcagcgtg
     6901 agggtccgct acgtcgccca aaccaaccgt accggcaccc cggcgtcggt attcggccag
     6961 ttggtggcca acttcgagct ggcaaaggtc gatccaaggc tggtaggcgt caacctcgta
     7021 gccccggagg acgaccgggt cgggttgcgc gactatagcc tccacatgtc gatgctggag
     7081 ttccttcacg acctggaccc gcaggtgaat atcaccctcc acgccggtga gctagccccc
     7141 ggcatcgccc cgtccgatgc attgtccagc cacatctggg aggcagtggt gaaggcaggc
     7201 gcccgccgga tcggccacgg cgccgacctg atgctggagc acgatcgcta cgacctgctg
     7261 gatgagatgg cccgccgcaa ggtgccggtg gagatcaatc tcacgagcag cgaactgatc
     7321 ctggagctga gcggcctgtc gcacccattg cccatgtaca tgcgcttcgg cgtgccggta
     7381 accctgtcca ccgacaatca gggggtgtcc aggggcggca tgaacaagga atacacccga
     7441 gcggcatcga cgtacgggct gtggtacgat gatctaaagc ggctggcacg caacggtatc
     7501 gagtacagct tcctcccggg agcaagcttg tgggaaaggg cggatgtctt cattccggtg
     7561 gcggcctgct ccaaggagcg cccatccacg acggagccct cttcggccgg ctgtaaggca
     7621 ttcctggaca ccagcgagaa ggcgcgccaa cagtggcggt tggaagcatc ctatgccgcc
     7681 ttcgagaacg gctactgagc gcagccccgt tcctgtaggg gcgacacatg cgtcgcccct
     7741 acaccacgac gcactccacg tgcgccacac gccctaaccg gagaaggtac tgcagatgca
     7801 actgattgga atcgatctcg ggacctcctt tctcaaaggt gccgttctgg atctggatcc
     7861 gccaagcatc ggtcacatcc acagagagcc tgtcccgaac cctgtgccgg gccttccgcc
     7921 cctctggaac gagtacgacg ccggcgagat cctcgcggcg acccgccggc tactgatcga
     7981 actggccccc ttcgccccgg actgcatcgg cttgatggta agcaaccaga tgagcagtct
     8041 ggtgctatcc gacggtgagg gccgcgctct atccaactgc atcggttggc gcgaccaacg
     8101 ctcactggcg tcgcatccct cgggggctgg ctcctacttc gacaggttgg cagagtcgat
     8161 tacaccggaa gagcggatcc agatgggcaa cgaacttcgg ccggggctac ctatcagctt
     8221 cctcgcctgg atggccgagg ccggctgctt gccctccggc gcaatcccag cctccctggg
     8281 cgatttcgtt gccgccagcc tgtgcaacac ggctccgtcc agcgacccga ccaacggctc
     8341 cgctcacggt acgctcaaca tcgagacggt ggattggcac taccctgtgc tggagaggct
     8401 ggggctgggc tccctgcgct ggccacagct acatcgaagc ggggacgtcg tcggcaccgt
     8461 ccggattggc gcacgagagg ttccctgcta cacccccatg ggcgaccagc aggtcgccct
     8521 ggccagcacg ttcctgaaac ccagggagct gtggctcaac atctccaccg gctcacaggt
     8581 cagtctcctg acccccaccc cccagttcgg ggcggcttac cgcacccgtc catcggcgga
     8641 tgggcgcttc tacaagacca tcacccacat acccggcggg cgctccatgg accagatcat
     8701 gaggctgctt tgcgaactgg cccaggcaga gggattgcag cttcgcgatc cgtggggata
     8761 tgctgctcat caggccgaac tgctggagca gaccgatctc aaggtgaacc tctccttctt
     8821 cctcagctcc tgcggcgacc gaggcgagat ctccaacatc ggggaggaga acctgaccgt
     8881 cggccacatc ttccgggccg cctatcagaa tatggccgac aactacgccg attgcgccct
     8941 gcgactctca ccggacagag aatgggatag tctggtcttc tccggcggct tggcgcagaa
     9001 gatggggcag ctgagaagac tgattgccga gaagttccag accggctacc gtatgacggc
     9061 atcctcagaa gatgccctgc tggggctact cgcgctgggg ttgctgttca ccggccaagc
     9121 gcgcaccctg gaggaagccg gcaggctgat ccccgaagaa cacatccagg agtctcgtta
     9181 gtgtacaagg cactagtttt cgatctggac ggcaccctgg tcgacacgcc cgcgggcata
     9241 gcccgcacca tagccagggt gctcgccaac cgtggatatc ctgttccgtc ctccgaggag
     9301 gtctaccaga tgatcgggct cccgctggtg gactcgttcc tccgcttcct ccccgccgac
     9361 gcccgctcta tcgcacccga atgtgttgtg gagtatcggg atctgtacga gcgcatcgag
     9421 attccggcga ccaccctttt ccccggggtg ggtgaaaccc tggcgcgatt ccaggctgcc
     9481 ggcgtgagaa tggcggttgc gaccgcgaag ctcacacgag tggccgaatc gacgctggca
     9541 cattccgggg ctcgggggta cttcgatgca gtcctcgggg gcgactgcgt gagcgagccg
     9601 aagccccatc cgcaactcgc tctgagcgcg ctggcgcggc tcgagtgctc gccggagagt
     9661 gcgctggtag tgggggatac gagctacgac atccagatgg gcaaggcggc gggcacagct
     9721 acatgtggag tgacctacgg agtgcagccc cgaacacagc tggcaggtgc gtcccccgac
     9781 tacctcatcg accgcttcgc cgagctcgcc gccatcttgg gcacagaata gactacatca
     9841 gcaaactcag cagcagctgc gccaggagga tcttcgcgat catcgccatg gggtagacca
     9901 tggcgtagcc gatgttgggc aactcgttcc tggtctgctc cagggagaag cccagcacgg
     9961 ccggctgcgt ctggagtccg gccaacatcc ccgtcagcag ccccatgggg atcttcatca
    10021 gcttgtagcc gatccacagt gttcccacgg cgaccgtcgt ggtgatcagc gcgccggctg
    10081 cgaagataac tg
//
LOCUS       DATALS010000330         5242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_259311, whole genome shotgun sequence.
ACCESSION   DATALS010000330 DATALS010000000
VERSION     DATALS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259311"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VHS06_07440"
     CDS             <1..962
                     /locus_tag="VHS06_07440"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX2987988.1"
                     /translation="GDGGVSAIVEEVDATPEQRRISEINSGLYCFDCPWLAANIGALR
                     KSPKGEFYLTDMVAIAVSQGRKVRALLVEDSSEVLGVNDRVQLADADRVARKRICEAL
                     MAKGVTIVDPSNSYIGKDVVVGADTVIQPGTHLRGKTVIGERCEIGPNSILDNAQVGD
                     DCTVLASVLEDARVEARVNIGPFSHLRPGAVIASGAKIGNYAEVKNSYLGEGVQMHHF
                     SYMGDAEIGDGTNIGAGTITCNFDGVKKHKTRVGKRVFLGSDTMLRAPIELGDGARTG
                     AGSVVTKNVPAGKLALGVPARIVEVKGQDDNDVDTHEGGESGG"
     gene            952..1875
                     /locus_tag="VHS06_07445"
     CDS             952..1875
                     /locus_tag="VHS06_07445"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX2987989.1"
                     /translation="MEARLQIFSGNSNGKLASEIVNNLGIPQGRAHVGTFEDGETWIR
                     LDENVRGSDVFLVQSLCTPTDHHIMELLLMVDAVKRSSASRITVVIPYMAYSKQEKKT
                     HGREPISAKLLADIISKAGADRFLSVDLHSPAIEGFFDIPVDHLRASAMLAHHVEKMY
                     LQDPVVVSPDTGGVDRANEMRERLGASLAIIYHERIEHDDCKTDECRRLEMVGDVNGR
                     PAVIVDDMISTGETVLMATEALLEAGATEVYACATHGVFTGNALDLIEASPIKKVIVT
                     NTVPVPPGAAGGKVQTLSVGSLIAESIMSVR"
     gene            complement(1881..2489)
                     /locus_tag="VHS06_07450"
                     /pseudo
     CDS             complement(1881..2489)
                     /locus_tag="VHS06_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
     gene            2640..3059
                     /locus_tag="VHS06_07455"
     CDS             2640..3059
                     /locus_tag="VHS06_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2987990.1"
                     /translation="MKQSSRQTPRDEATVLIIEDDPPSARMLQFLLENDGLRTRVATD
                     GRTALASYMKETPDVVLMNLVMPDADGFEIHHRLRSLGYRGPVAFVTARPDAWQIIRR
                     RGADVAACLVKPFYPDDVVRMVRSLVRTNRKTLRPTA"
     gene            complement(3195..>5242)
                     /locus_tag="VHS06_07460"
     CDS             complement(3195..>5242)
                     /locus_tag="VHS06_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX2987991.1"
                     /translation="ILMAALGVFAIIQLGIVNECSTEIAEGWLPSTRYLGDFRAASEQ
                     LRSREFQHNIATDAAELASLEKKMDETRQEADDALKNYEKILSGEEDRKLFSEMKQSW
                     TAYLETHEKRFLAASRANDSKKASEVLDGDMKKQFDALRENVDKVTQFNIAGGTAASA
                     RGDELFASSRTMTIGAILVALVISTSLATWLSRNISRGVMAVAMAARQIAQTDLPQLA
                     SCSQSIAEGDLTKNLAVTCQPVQVKSNDEIGDMARSFNDMIARLRETGDAFGKMTANL
                     RGTIGTVASAAVSLTEASQQLAAASEQSGAAAQQIATTIQEVAKGNQDQSASVQETTA
                     SVDQLGRAIDQIAKGSQNQSRAVEHAAASVTQLNDSIQQANTASKGLSAAGDEVTSAA
                     RNGAKSVQSTAEGITAIKESTALAASKIQELGAYSDQIGSIVETIDDIAEQTNLLALN
                     AAIEAARAGEHGRGFAVVADEVRKLAERSSKSTKEIAELIGQVQKATHEAVSAMEQGG
                     KEVEHGALQSRESADAISSVLAAVEKTNLEVSRIAGALLEMETASGQVVAQMDSVSSV
                     VEETSVFTKEMAASSGQVTGAIEKIAAVSEQTSASAEEVSASAEEMSAQVEEMVAESQ
                     ALAQMAEGLQAEVARFKLEDDAGGAEVSLRRRKADWAPETPREATAPRRRAEAVSRS"
BASE COUNT         1031 a         1554 c         1587 g         1070 t
ORIGIN      
        1 atggggatgg tggtgtcagc gctatcgtgg aggaggtcga cgccacgccg gagcagcgga
       61 ggatatcgga gatcaacagc gggctctact gtttcgactg cccctggttg gcggcgaaca
      121 taggtgcgct ccgcaagagc cccaagggcg agttctatct taccgacatg gtcgccatcg
      181 cggtgtctca gggccgaaaa gtccgtgcgc tgctggtgga ggattccagc gaggtactgg
      241 gagtgaacga tcgagtccaa ctggccgacg cagacagggt cgccaggaag cggatctgcg
      301 aggcattgat ggcaaaggga gtcaccatcg tcgacccctc gaacagctac atcggcaagg
      361 atgtggtggt aggtgcggac acggttatcc aaccgggcac ccatctgaga ggcaagacgg
      421 tgatagggga aaggtgcgag atagggccga actcgatcct ggacaacgcc caggtcggag
      481 acgattgtac ggttctcgcc tctgtcctcg aggacgccag ggttgaggcg agggtgaaca
      541 tcggaccctt ttctcaccta cgccccggag cagtgatcgc ctcgggagcc aagataggca
      601 actacgccga ggtgaagaac agctatctgg gcgagggcgt ccagatgcac catttcagct
      661 acatgggaga tgcggagatc ggggacggca cgaacatcgg agccggaacc atcacctgca
      721 acttcgacgg tgtgaagaag cacaagaccc gcgtcggcaa gcgggtcttc ctgggaagcg
      781 acacgatgct gcgtgcccct atagaactcg gagatggggc ccgcaccgga gccgggtctg
      841 ttgtgaccaa gaacgttccc gctgggaaac tggccttagg ggtcccagcg cggatcgtgg
      901 aagtgaaggg gcaagacgac aacgacgtcg acactcatga agggggagaa agtggaggct
      961 aggcttcaga tattctccgg caactcgaat ggtaagcttg catcggagat agtcaacaac
     1021 cttggtatac cacagggcag ggcccacgta ggcaccttcg aggacggaga aacctggatc
     1081 cggctcgacg agaatgtgcg aggctcggac gttttcctcg tgcagagcct ttgtacgcca
     1141 acagaccatc acatcatgga actgttgctg atggtggatg cggtgaagcg atcctcggct
     1201 tcgcgtatca ccgtcgtcat cccctacatg gcctattcca agcaggagaa gaagactcac
     1261 ggacgagagc cgatctcggc aaagctgctg gccgacatca tcagcaaggc gggcgcggac
     1321 aggttcctct cggtggacct tcactccccc gcgatagagg gtttcttcga catcccggtg
     1381 gaccatctgc gggcatcggc aatgctggct caccacgtgg agaagatgta tctccaggat
     1441 ccggtggtcg tctcgcccga taccggaggg gtggacagag ccaacgagat gagagaaagg
     1501 ctgggcgcgt cccttgccat catctaccac gagcggatag aacacgacga ctgcaagaca
     1561 gacgagtgcc gcagactgga gatggttgga gacgtcaacg gcagaccggc cgtgatagtg
     1621 gacgacatga tctcgacggg tgagaccgtg ctgatggcaa cggaggctct gctggaggcc
     1681 ggcgccaccg aggtatacgc ctgcgcgact cacggcgtat tcactggcaa cgccctggat
     1741 ctgatcgagg cttcgccgat caagaaggtt atcgtgacca acaccgtacc ggtgcccccc
     1801 ggtgcagcag gtggaaaggt gcagacactg agcgtagggt cgctgatcgc ggagtcgatc
     1861 atgtcggtaa gatagaactc gcccttgggg ctcttgcgga gcgcacctat gttcgccgcc
     1921 aaccaggggc cgtcgaaaca gtagagcccg ctgttgatct ccgatatcct ccgctgctcc
     1981 ggcgtggcgt cgacctcctc cacgatagcg ctgacaccac catccccatc tcggatgatc
     2041 ctaccgtagc catgtggggc ctcgacgttg gcggtgacga caaccatggc cgcgtcccct
     2101 agttgggagg agagtttcga gatggtatcg gccgaaagaa gtggtgtgtc tccgcacagc
     2161 acgagcagcc tggccggaat gaggtccata agttgcagcc ccacctgaac ggcatggcct
     2221 gtcccgagtt gaggctgctg gaggacaaaa cgaaccgagc taccgccgac cgccgatatc
     2281 acctgctcgc tgttgtgtcc caccaccagg acgatgtcgg ctatgccggc tccgcggatg
     2341 gaatccaaca aatgaaacag gacggggtcg cccgcaactc gatggaggat cttgtggagg
     2401 ccggagcgca ttcgtgtacc catgcccgcg gctagcacca gggcagttga gtctggcatc
     2461 cggcttgctt gttgtggaat gtcgtccata aactccgact atctctatgg ggatgttacc
     2521 ttcagcccat catcctgtca agacaacagc ggtggcgctc caggcgcgaa aactggcatc
     2581 gatcctgcaa gttgttgtat tagctccgta tcagcatcct gtggaggtct taactcccta
     2641 tgaagcagtc ttctcgtcag actccgcgcg acgaggcaac cgttctgatt atagaagatg
     2701 acccaccctc cgcacgaatg cttcagttcc ttctcgagaa cgatggcctg cgcactcgtg
     2761 tggccacaga tggacgcact gctctggcct cctacatgaa ggaaacgccg gacgttgtcc
     2821 tgatgaacct cgtgatgccc gacgcggacg gcttcgagat acaccacaga ctgcgcagcc
     2881 tcggctaccg ggggccggta gcgttcgtga ccgcgagacc ggatgcatgg cagatcatac
     2941 ggcgacgggg agcggacgtc gcggcctgcc tggtcaagcc gttctatccg gacgacgtgg
     3001 tcagaatggt gaggtctctg gtccgcacaa atcgaaaaac ccttaggccc acagcgtaaa
     3061 gacgagagag tggattgaga gctacgctgg acactggcga agttgggatg gacccgggga
     3121 ggcagaaccc ctttcgccca tcccaacttc gccagtgtcc tatgtaaaca ctgttggcgt
     3181 tgggttcccg ctagttacga gcgggagacc gcttcggccc ttcgccttgg ggccgtcgct
     3241 tcccgaggtg tctcaggggc ccagtcggct ttccgtcgcc gcagactgac ttcggcgcca
     3301 ccggcgtcat cctccagttt gaatcgggcg acttccgcct ggagcccttc cgccatctga
     3361 gccagtgctt gagactcggc gaccatctcc tccacctgag cgctcatctc ctcagcggag
     3421 gcgctgacct cttcggcgga ggcactggtt tgctcgctaa cggccgcgat cttctctatc
     3481 gcccccgtta cctgtccgga agaagctgcc atctccttgg tgaagacgga agtctcctcg
     3541 acgacggagg agacagaatc catctgggcc actacctgtc cagatgcggt ctccatctca
     3601 agcagtgcgc cggcgattct ggatacctcc agattggtct tttcgacggc ggcaagaacg
     3661 ctggagatcg catcggccga ctcgcgcgat tgcagagcac catgctctac ctcttttcct
     3721 ccctgctcca tcgccgaaac agcctcgtgg gtcgccttct gcacctgccc gataagctcg
     3781 gctatctcct tggtgctctt ggacgaccgt tccgccagct tcctcacctc gtccgccacc
     3841 acggcgaagc ctctcccatg ttcgcccgcg cgagccgctt cgatggctgc gttgagggca
     3901 aggaggttgg tctgctctgc tatgtcgtcg atggtctcga cgatcgatcc aatctgatcg
     3961 gaataggccc ccagttcctg gatcttggag gctgccagag cggtgctctc tttgatggcg
     4021 gtgatccctt ctgccgtgct ttgcaccgac ttcgccccat tccttgccgc ggaggttact
     4081 tcgtcaccag ccgccgatag tcccttggag gcggtgtttg cctgttgtat cgagtcgttc
     4141 agttgggtga ccgaggccgc ggcgtgctcc actgccctgg actggttctg ggagcccttc
     4201 gcgatctggt cgatggcgcg gcctagttga tccaccgagg cagtggtctc ctggacggag
     4261 gccgactggt cctgattgcc ctttgccact tcctggattg tggtagcgat ctgctgggca
     4321 gccgctccgc tttgctcgga agccgcggca agttgctggc tcgcctctgt cagggagacg
     4381 gcggcggaag cgacggtgcc gatggtacca cgcaggttgg cggtcatctt gccgaaggcg
     4441 tctccagtct cccttagcct cgcgatcatg tcgttgaagg agcgggccat atcgccgatc
     4501 tcgtcgttgg acttcacctg aactggctgg catgtcacag ccaggttctt ggtgagatcg
     4561 ccttcggcaa tcgactggga acaggaagcg agttgcggga gatccgtctg cgctatctgc
     4621 ctggcagcca tggcaaccgc catcacgccg cgggatatat tgcgggacag ccacgttgcc
     4681 agagaagtgg agatgaccag tgccacaagg atcgcgccga tggtcatcgt tctacttgag
     4741 gcgaatagct catcgcctcg ggcgctggcg gctgtacccc ccgcgatgtt gaactgggtt
     4801 actttgtcga cattctctcg cagcgcgtcg aactgcttct tcatgtcgcc atcgagcacc
     4861 tccgaagcct tcttgctgtc gttggcacgg gatgccgcta gaaaccgttt ctcatgggtc
     4921 tctagataag cagtccagct ctgcttcatc tcgctgaaca gcttcctatc ttcttcacca
     4981 ctcaagatct tctcgtaatt cttgagggca tcatcagcct cttgcctcgt ctcgtccatc
     5041 ttcttctcaa gactggccaa ctccgcagcg tccgtagcga tattgtgctg gaactccctg
     5101 gaccttagct gctcggatgc cgccctgaaa tctccaagat accttgtcga agggagccag
     5161 ccttcggcaa tctcagtgct gcattcgttc acgatcccca attggatgat cgcgaagacg
     5221 ccgagggcag ccatgaggat cc
//
LOCUS       DATALS010000331         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_392521, whole genome shotgun sequence.
ACCESSION   DATALS010000331 DATALS010000000
VERSION     DATALS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392521"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1240
                     /locus_tag="VHS06_07465"
     CDS             179..1240
                     /locus_tag="VHS06_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX2987992.1"
                     /translation="MHRVTLIPGEGIGPEVTSVARRVLDASGVQFDWDVVEAGGHVME
                     RYGTPLPDHVLDSLQGTGVGLKGPITTPIGKGFSVPVNWTGGGRRPGVTRVYPSVNVA
                     LRKELDLYAAVRPARNYPGFPTRFTGVDLVVVRENTEDLYMGIEHAVTPDVAEAVKII
                     SRGASERVVRFAFDFAVKQGRHKVTAVHKANILKLTDGLFLEAAQEVSRAYPHVQFET
                     RIVDAMCMQLVQTPETDDVIVAPNLYGDILSDLCAGLVGGLGVAPGANVGGAKALFEP
                     VHGTAPTIAGQDKANPVAAILCGVMLLRHLGEAAAADRVDRAVSTVLKERKTVTADMG
                     GSAGTRQMGDAIVGALQQS"
     gene            complement(1398..1904)
                     /locus_tag="VHS06_07470"
     CDS             complement(1398..1904)
                     /locus_tag="VHS06_07470"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEX2987993.1"
                     /translation="MGTESRDQGSGNVPNRAQRLRGVTRSWLNAGEDIILVGVAVILL
                     LAGLLVIANAVSGFASALSSSSIAQTVFGVAESALLALILAELVHSLLVSLDGGGLTL
                     EPFLVIAIVAILRKMLLTTVLIPSPTEAQGLITPSVAELLALGLVILALTVALAISRR
                     HGNQARPE"
     gene            complement(1950..>2294)
                     /locus_tag="VHS06_07475"
     CDS             complement(1950..>2294)
                     /locus_tag="VHS06_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987994.1"
                     /translation="GGPQQPPQGNFNPGQGGPGGGGGMFNTGQPGPLRLFTGSLAKNI
                     AWLLPFGLFGAVLLAFRARPRWAIAQKHQAMVLWGGWLVTGGVFFSVAGFFHEYYLSM
                     LTPPLAALVVDH"
BASE COUNT          436 a          679 c          749 g          430 t
ORIGIN      
        1 attgtagcac cgcctgtgat ggtgctccac gatttttcgg gaaatgtggc cgttggaggc
       61 gtcttcgtct gaggcaacga gagcttggat gaggaatcta ctgcatcgta gcacggtgcg
      121 ggcaaactac ttgcctacat gtgtgaatcg cgtgcctcgc ggcgaaggag gactccaaat
      181 gcatagggtg accttgatcc ccggcgaggg catcggccct gaggtgacga gtgttgcacg
      241 gagggtcttg gacgcgagcg gcgtccagtt cgactgggat gtggtggagg ccggcggcca
      301 tgtaatggag aggtacggca ccccacttcc cgatcacgtc ttggactccc tccagggcac
      361 cggcgtcgga ttgaaggggc cgatcaccac ccctatcggc aagggcttca gcgtgccggt
      421 taactggacc ggggggggaa ggcggcccgg cgtcacccga gtgtatccca gcgtgaatgt
      481 ggcgttgagg aaagagttgg acctgtatgc tgccgtgcgc cctgctcgaa actaccccgg
      541 gtttcccacc aggtttacgg gagtcgacct ggtcgtggtg cgcgagaaca ctgaggacct
      601 gtacatgggc atcgagcatg ccgttacgcc tgatgtggcc gaggcggtca agatcatatc
      661 ccgcggtgcc tccgagaggg tggtgcgttt tgccttcgac tttgccgtca agcaggggcg
      721 acataaggta accgcggtac acaaggcaaa catcctgaag ctgaccgacg gtctgtttct
      781 cgaggccgcc caggaggtct ctcgtgccta cccgcacgtg cagttcgaaa cgcgtatcgt
      841 ggatgccatg tgcatgcagc ttgtccagac tcccgaaacc gatgacgtga tcgtggctcc
      901 caatctgtac ggagacatcc tttccgacct gtgtgccggg ctggttggag gactcggcgt
      961 ggcgccgggg gcgaacgtgg ggggagcaaa ggcgctcttc gagccggttc atgggactgc
     1021 tcccaccatc gcggggcagg ataaggccaa cccggttgcg gccatcctgt gcggcgtgat
     1081 gctactcagg catctgggcg aggctgcggc cgccgatcgt gtggatcgtg cggtctcgac
     1141 ggtcctgaag gagcgcaaga ctgttaccgc cgacatgggc ggatcggccg gcaccagaca
     1201 gatgggcgat gccattgtgg gtgcattgca gcagtcctaa tcggactacc ccttgccccg
     1261 ggtcaggatg agtgccacgg ccgccacgaa catggttgcc gttgtcactc agcctaccag
     1321 cttcgtgaag aggctgttgg cgtatccgtg cataaccagc ctgttgtttg cgatgaccat
     1381 tatcatcaaa ggaacggtca ttcgggtctc gcctggttgc cgtgccgacg actaatggcc
     1441 agagccaccg tcagcgccag tatcaccagc cccagtgcga gcagttcggc aacagagggg
     1501 gtgatcaacc cctgagcttc ggttgggctg ggtatgagca ccgttgtgag cagcatcttt
     1561 cgcaggatgg cgacgatggc gatgacgagg aacggctcga gtgtgaggcc accaccgtcg
     1621 aggctgacca acagactgtg aacgagctct gcaagaatca gggcgagcag ggcgctctcg
     1681 gcgacgccaa agactgtctg ggcgatcgaa ctggaggaca gtgcgctggc gaaaccgctc
     1741 accgcgttgg cgatgaccag caagccggcc agtagcagga tgacggccac gccgacgaga
     1801 atgatgtcct cgcccgcgtt tagccaactc ctggtgacgc ctcgtagccg ctgggccctg
     1861 ttagggacgt ttccgctgcc ctggtctcga ctttcggtcc ccataccctc ccccgatgtg
     1921 gtacctagtc gtctcctgtc ccccacacat caatgatcaa ctaccagcgc ggccagcggc
     1981 ggggtcagca tggacaggta gtactcgtgg aagaagcccg ccacgctgaa gaatacccct
     2041 ccggtgacca gccatccgcc ccacagaacc atcgcctggt gtttctgggc gatcgcccac
     2101 cttggccggg ctcggaaggc aagcagaaca gcaccaaaga ggccaaaggg aagcagccag
     2161 gcgatgttct tggccagaga cccggtgaac aacctcagcg ggcccggctg gccggtgttg
     2221 aacatgcctc ctccaccgcc gggaccaccc tgccccgggt tgaagttgcc ctggggcggc
     2281 tgttgcgggc cgcc
//
LOCUS       DATALS010000332         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_392617, whole genome shotgun sequence.
ACCESSION   DATALS010000332 DATALS010000000
VERSION     DATALS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392617"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..1293
                     /locus_tag="VHS06_07480"
     CDS             349..1293
                     /locus_tag="VHS06_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987995.1"
                     /translation="MLARHGMAPFLAAAGTSQPLFAGLIMLARQQHDAFSSLLGLSSF
                     VGLVICVADRKLLMPIWFISTFLLDSRTAHTTANVPIALLAGMGLAHLLTHLIHTRTI
                     QTSRGDSLGLARHPAVQIVLAILLAQAMLSDLTVLGPRMQSLSREEREAMSWIADNTP
                     ISSTFAVVTSIGSSYDSTSEWFPTQAHRVSVATAQGYEWLGTFDDRLRWHWKLQQCGT
                     SNADCLDHWMGATGAAFTHVYVAGSPSSENRGVDPRGALRDSLARDPRYTRVHDGPGA
                     TVFRRVNPEDPIANGEPASLEQHVTGTYAAAGSGENVQ"
     gene            1290..>1718
                     /locus_tag="VHS06_07485"
     CDS             1290..>1718
                     /locus_tag="VHS06_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2987996.1"
                     /translation="MILRSRSAVQTQPIATLPVRVLGGLHQRRLLAILLFLALFSYDF
                     VRPTDIDFWWHLKTGELIATTGTIPTADPFSYTVPGRPWIAHEWLWELAIYLIYSRGG
                     YMTAALLSAAAVTLTYIILYRLLRKLGTSEFVAAALVLWAA"
BASE COUNT          290 a          601 c          495 g          332 t
ORIGIN      
        1 tatcggcaat ccagctcatc gcctcgcgtt cctcgcggga gaggctttgc atcctgggtc
       61 caccctgcgc gcgaccgact cgatcgcgtg gcgggcgtcg tacaccgggt atgtcgctgc
      121 cgtattgctg gaaaggcggc cgcagtcctc gtgggatgag aactcttgcc tcatgccccg
      181 tcccgccgga cgaagatctg ctcgacgtcg ccctcgtaca ccttctgcca atccgactgg
      241 ctcgctagga ggacggcgag gggacagtcc ttctcgatca gcacggtcgc gcgctctatc
      301 gttatggggc tcttggcggt gaccttgtcg gctccctggt ggggtacagt gctggctcgc
      361 cacggaatgg ctccgtttct ggcagcggca ggaaccagtc agcccttgtt cgcagggctc
      421 atcatgctgg cgcggcaaca gcacgacgca ttctcgtccc tgctcggtct gtcgagcttc
      481 gtcggcctcg tgatctgcgt agccgatcgc aagcttctca tgccaatctg gttcatctca
      541 actttcctcc tagactcccg gaccgcccac acgaccgcaa atgtgccgat tgccttgctt
      601 gccggcatgg gcctcgcgca cctcctgaca cacctcattc atacccgaac gattcagacc
      661 agccgcggcg acagtctcgg tctcgcgcga cacccggccg tgcagatcgt tcttgccatc
      721 ctcctcgccc aggcaatgct ttcggatctc acagttctcg gacccaggat gcaaagcctc
      781 tcccgcgagg aacgcgaggc gatgagctgg attgccgata acaccccgat atccagcaca
      841 ttcgccgtcg tgacctctat cgggtccagc tatgattcga cctcggagtg gttccccacc
      901 caggcccacc gcgtaagcgt agctaccgcg cagggctacg agtggctcgg caccttcgac
      961 gaccgccttc ggtggcactg gaagctgcag cagtgcggca cctccaacgc agattgcctc
     1021 gaccactgga tgggagcgac cggggctgca ttcacgcacg tctacgtggc gggctcaccg
     1081 tccagtgaga accgcggggt cgatccacgc ggagccctgc gcgattcgct ggcgcgcgac
     1141 cctcgataca ctagagtcca cgatggccca ggcgcgacag ttttccgtcg agtgaaccca
     1201 gaggacccga tcgccaacgg agagccggca tcgctggaac aacatgtcac cggcacgtat
     1261 gcggcagccg ggtcgggaga aaacgtgcag tgatactcag gagccgatct gccgtccaga
     1321 cccaacctat cgccaccctc cccgtcaggg tgctgggtgg cctgcaccag cgccgcctgc
     1381 tcgccatcct gttattcctc gccctattct cctatgactt cgtccgcccc acggacatcg
     1441 acttctggtg gcacctgaag accggcgagt tgatcgccac gaccgggacc ataccaacgg
     1501 ccgacccatt ctcctacacc gtccccggcc ggccatggat cgcccacgag tggctgtggg
     1561 agctggcgat ctacctgatc tacagccggg gcgggtacat gacggcggcc ctcctgtcgg
     1621 ctgccgccgt cacgctgacc tacatcatcc tgtatcgact tctccgcaag cttggaacga
     1681 gcgagttcgt ggccgcggcg ctcgtcctgt gggccgcc
//
LOCUS       DATALS010000333         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_259701, whole genome shotgun sequence.
ACCESSION   DATALS010000333 DATALS010000000
VERSION     DATALS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259701"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..760
                     /locus_tag="VHS06_07490"
     CDS             113..760
                     /locus_tag="VHS06_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX2987997.1"
                     /translation="MRIVIVGCGRVGAELSTDLSIHGHEVAVIDREPAAFSRLGRAFK
                     GKTVTGIGFDRDVLLESGVDRADAFASVTSGDNTNIVSAMIARNVFRVPKIVARIADP
                     QRAEIYRRLGIPTISPTIWGANEIRELLITPDLTSRFTFGSGEVELIQFHASSRMAGY
                     SVADLTSPSQFSVVSIVRGGRAFLPTAGSQLEEGDLVYVASEAGSLPKLEEIIRA"
     gene            832..1254
                     /locus_tag="VHS06_07495"
     CDS             832..1254
                     /locus_tag="VHS06_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX2987998.1"
                     /translation="MQVLVVGCGRIGNYLVEELLAKGHQVAVVEKDPGRCEELARQDR
                     SNALRIVYGDGNDPMVLREAGARDADALAAATGEDEDNLVIGLLGKREFGIRRVIGRV
                     NNPRNAWLYSAAFGFDAAVRPAETIVSLIEEQIESPAP"
     gene            complement(1330..>1586)
                     /locus_tag="VHS06_07500"
     CDS             complement(1330..>1586)
                     /locus_tag="VHS06_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX2987999.1"
                     /translation="NLVIGLLGKREFGIRRVIGRVNNPRNAWLYSAAFGFDAAVRPAE
                     TIVPADHRAVAVEATVGVAGLCADRAVGAGSRSPAGDRRR"
BASE COUNT          334 a          477 c          528 g          247 t
ORIGIN      
        1 taatcgccat gatggtcgca ttcgcggtgt gggaggcggg gcacaagacc ccgccctacg
       61 agacgtcaga ggtgacaaag acaggtcagc ggatgatgga tggaggcaac atatgaggat
      121 agtgatcgtg ggttgcggac gcgtgggggc cgagctatca accgacctct ccatccatgg
      181 gcacgaagtc gcggtgatcg acagggagcc tgccgccttc tcgcgccttg gcagggcatt
      241 caaagggaag accgtcaccg gcatcgggtt cgaccgcgac gtcctgctgg agtccggcgt
      301 cgaccgggcg gatgccttcg cgtcggtgac cagcggcgac aacaccaaca tcgtctcggc
      361 catgatcgcc cgaaacgtat tcagagtgcc gaagatcgta gcccgcatcg cggaccccca
      421 gcgagcagaa atctaccgcc ggctggggat accaacgatt tcccccacta tctggggagc
      481 gaacgaaatc cgcgagttgc tgatcacccc cgatctcacc agccggttca ccttcggcag
      541 tggcgaggtg gagctgatcc agttccatgc ctccagtcga atggcgggct acagcgtggc
      601 ggatctgacg agcccatccc aattcagcgt ggtctccata gtcaggggtg ggagggcatt
      661 cctccccacg gccggaagcc aattggaaga gggcgacctg gtgtacgtgg caagtgaggc
      721 gggatccctg cccaagctgg aagagatcat ccgcgcatag cagacaacgg catgagaagg
      781 gacacaagcc tccggtcgta gcggaagaca caagcacggg ggaggggcac gatgcaggta
      841 ctggtcgtgg gctgcgggag gatcggcaac tatctggtcg aggaactgct ggccaaggga
      901 caccaggtag cagtggtcga gaaagacccc ggacgctgcg aggaactggc caggcaagat
      961 aggagcaacg cgctgcggat cgtctacggg gacggcaacg accccatggt gctgcgagaa
     1021 gcgggagcga gagatgcgga tgcactggcg gcggccacag gtgaagacga ggacaacctg
     1081 gtaatagggc tgctgggaaa gcgagagttt gggatccgca gggtgatcgg cagggtgaac
     1141 aacccacgca acgcctggct ctactccgcg gccttcggct tcgacgcggc ggtaaggccg
     1201 gcggagacca tcgtgtcgct gatcgaggag caaatcgagt caccggcccc ctgatagggg
     1261 attgggggtt ggggggggca gtctaccagg tcaggctctg cgtgcaaccc aacccccaac
     1321 ccctatcccc tatcgccgtc tgtcaccggc tggcgaccgc gaacccgctc ccacagcacg
     1381 gtcagcgcaa agaccggcaa caccaactgt cgcttccacc gccacggctc ggtgatcagc
     1441 cggtacgatg gtctccgccg gccttaccgc cgcgtcgaag ccgaaggccg cggagtatag
     1501 ccaggcgttg cgcgggttgt tcaccctgcc gatcaccctg cggatcccga actctcgctt
     1561 tcccagcagc ccgatcacca ggttgt
//
LOCUS       DATALS010000334         4174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_422652, whole genome shotgun sequence.
ACCESSION   DATALS010000334 DATALS010000000
VERSION     DATALS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422652"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VHS06_07505"
     CDS             <1..281
                     /locus_tag="VHS06_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HEX2988000.1"
                     /translation="HYGSPIPSCPESQADGCMTGTGTVLGTYLHGLFENRSLRDALVN
                     WLIERRGLPPIRWEGPEASRDAEYDRLAECLRHHLDLPAVYTIMGLGR"
     gene            285..863
                     /gene="cobU"
                     /locus_tag="VHS06_07510"
     CDS             285..863
                     /gene="cobU"
                     /locus_tag="VHS06_07510"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX2988001.1"
                     /translation="MGSLVLILGGARSGKSTMAEKLALAAGGQVTYVATAEPRDDEMR
                     WRIEHHRAARPAGWRTVEEPLDLVTAVERAAAESDVVLVDCLTLWLSNHLCLCDAPES
                     SEEWAAAERALADNLERWTTSLVETARCHEVTLLLVSNEVGLGLVPANALGRIYRDLL
                     GLVNRRLAAEADRVLLMVAGLPLDVKRMAELG"
     gene            878..1807
                     /locus_tag="VHS06_07515"
     CDS             878..1807
                     /locus_tag="VHS06_07515"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEX2988002.1"
                     /translation="MRHAVVLVPCTCGELVQGMVGGEPFLVSCPIDRYSRVRVTLGMA
                     PPSNRLTSAKAVRAAAATLESIGLGGEPFTLEASCPLPPSRGFGTSTADVAGAIVATA
                     AAAGVALSPTEVARLAVAVEPSDSSMFPGLGLLAHRTGRLSESLGPAPSLLMVVLEFH
                     GDVDTLEYNARLNLDRLRSMEPDHLRALDLLREGLRMREPELVGEAATLSARANQPFL
                     PKPELETVIALGRDCGALGICAAHSGTVLGVLFEPGAGRKARKLLGMAGSRIEGLSNS
                     WMTRMVGGGVGILAVSDQNERFPTPVAAIVPSM"
     gene            1818..2867
                     /gene="cobT"
                     /locus_tag="VHS06_07520"
     CDS             1818..2867
                     /gene="cobT"
                     /locus_tag="VHS06_07520"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX2988003.1"
                     /translation="MTDLDRVLASIQSLEESAMEAARQRQNQLTKPTGSLGRLEDLAV
                     QLAGITGQVQPRLPRKAVVVMAGDHGVTADGVSAYPAEVTPLMVLNFLKGGAAINVLA
                     RQAGARVVVVDVGVAADLPPHEALVSRKVAKGTRNLAIEPAMTPEEARRAIQVGIEVL
                     ESEVAKGLDVVATGEMGIGNTTPSSAIVAVLTGLPVAEVAGRGTGVDDAGLARKVRAI
                     EQGISATRPDASDAMDVLTKVGGLEIAALVGVILAGAAHRLPVVIDGFISGAAALVAV
                     RLCPAVRPYLVPSHQSVEVGHRAILRDLGLRALFDLDLRLGEGTGAVLAMHLIDDALA
                     LHSEMATFAEAGIGE"
     gene            complement(2934..3581)
                     /locus_tag="VHS06_07525"
     CDS             complement(2934..3581)
                     /locus_tag="VHS06_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2988004.1"
                     /translation="MGWLENILGRTGPSDEYKKATALNKKAIDRIAHNHLDEAEKLLQ
                     EAIQIAPDHAAAHHNLGTVYLTQRKLAQALKFMRKAVRLDPADIESQIAVAKIYNDMG
                     KKEESLAEYARICEVAPNDWRSRISYGNALLERNRLEEALPHLEQAVEMNKKEELTHL
                     VLATAYERRGDLDKAIQQYRAVRNTTKVGQNRAAASQKMKELQRRRAGEQVANRE"
     gene            3708..4127
                     /locus_tag="VHS06_07530"
     CDS             3708..4127
                     /locus_tag="VHS06_07530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2988005.1"
                     /translation="MVSGSARSRSILFFSVVTTLYWSSMYIYVPTLSVHAENLGASVA
                     LVGMIVASYGFPQILVRIPMGIWSDRIGVRKPFILAGIAISTLAGLGLALTSDPALLV
                     VWRAMSGVGAPAFPMETRCWREIASRRRFLTWSRLWR"
BASE COUNT          691 a         1205 c         1453 g          825 t
ORIGIN      
        1 ctcattatgg ttccccaatt ccgagttgcc cggagtcgca ggcggacggg tgcatgaccg
       61 gtacggggac ggtgctaggc acctacctgc acggtctttt cgagaaccgg agtctccgcg
      121 acgccctggt gaattggttg atcgagcgtc gggggctccc gcctatccgt tgggaggggc
      181 cggaggcgag ccgggacgcc gaatacgatc gattggcgga gtgcttgcgc caccacctcg
      241 atctgccggc cgtctacacc atcatggggc tggggaggta gcccgtgggc agtctggttc
      301 tgatcctggg gggcgcgcgc agcggcaaga gtaccatggc cgagaagctg gcactggcgg
      361 ccggaggcca ggtcacctac gtggcgacgg ccgagccccg cgatgacgag atgcgctggc
      421 gcatcgagca tcaccgcgca gccaggccgg cgggatggcg aacggtggag gagccgctgg
      481 acctggtcac cgcggtcgag agggcagccg cggagagcga cgttgttctg gtggactgcc
      541 tgaccctgtg gctctcgaac cacctctgtc tctgcgatgc gcccgaatcg agcgaggagt
      601 gggccgcagc ggagagagcg ttggccgaca acctcgagcg gtggaccaca tctctcgtgg
      661 agactgcccg gtgccatgaa gtgaccctgc tgctggtgtc caacgaggtg gggcttggat
      721 tggtgccggc caacgccctg ggacgcatct atcgggatct tctgggattg gtgaaccggc
      781 ggttggcggc cgaagccgat agggtactgc tgatggtggc gggcctgccg ctggacgtta
      841 agcggatggc agaactgggg tagcgatggt gagccggatg cggcatgcgg ttgttctggt
      901 tccctgtacc tgcggtgagt tggtgcaggg catggtgggc ggtgagccgt tcctggtctc
      961 ctgccccatt gatcgctaca gtcgtgtccg tgtgacgctc gggatggctc cgccttcgaa
     1021 tcggctcact tccgccaagg ccgtccgggc cgccgcagcc actctggagt cgattgggct
     1081 cggtggtgag cccttcacgc tggaggcatc atgccctcta cccccctccc ggggcttcgg
     1141 gaccagtacg gcggatgtgg ccggagcgat cgtggcgacc gccgcggcgg cgggggtggc
     1201 cctgagcccg accgaggtcg cgagacttgc ggtagccgtg gaacctagcg acagcagcat
     1261 gttcccgggg ctggggctgc tggcgcaccg gacagggcgt ctgtcggaga gcctcggacc
     1321 cgctccctcg ttactgatgg tggtgctgga gtttcacggc gatgtagata cgctggagta
     1381 caacgccagg ttgaacctgg atcgccttcg atccatggag cccgaccacc tgcgtgccct
     1441 cgacctgctg cgagaagggt tgcggatgcg cgagccggag ctggtgggag aagcggcaac
     1501 gctgagcgct cgagcgaacc agcccttcct tccgaagccg gaactggaga cggtgatagc
     1561 cctcggtcgg gactgtggag cccttggaat ctgcgctgcg cacagtggaa ccgtgctggg
     1621 cgtgctattc gaaccaggcg caggacgaaa agcgaggaag ttgctgggca tggccggcag
     1681 ccggatcgag ggtctcagca actcctggat gacgcggatg gtcggaggcg gagtcgggat
     1741 tctggcagtc tccgaccaga atgagcgctt tcccacaccg gtggcagcga tagtccctag
     1801 tatgtgaggt ggtagaagtg acggacctgg accgcgtcct cgcatccatt caatcgttgg
     1861 aagagtctgc catggaggcg gcacgacagc gccagaatca gttgaccaaa cccacgggat
     1921 cccttgggcg gctggaggat ctggcagtgc agttggcggg catcaccggc caagttcaac
     1981 cgagactccc caggaaggcc gtggtggtga tggccggcga ccatggggtc acggccgacg
     2041 gagtctcggc ctatcctgct gaggtgaccc cgctgatggt gctgaacttc ttgaagggcg
     2101 gggcggccat caacgtgctg gcccgccagg ccggtgcgag agtggtggtg gtggacgtcg
     2161 gagtggcggc ggaccttccg cctcacgaag ctctggtgtc ccgaaaggtg gctaaaggaa
     2221 ccaggaacct ggctatcgag ccggccatga ctccggaaga agcgaggcgg gcgatccagg
     2281 tggggatcga ggtactggaa tctgaggtag ccaagggact ggacgtggtg gcgacgggcg
     2341 agatggggat cggcaacacc acgccctcct ccgcgatcgt ggcggtccta accggtttgc
     2401 ccgtggccga ggtggcgggg cgtggcaccg gcgttgacga cgccggcctc gccaggaagg
     2461 tgcgcgccat cgagcagggg atcagcgcta cgcgacccga tgcctcggac gcgatggatg
     2521 ttctgacgaa agtgggggga ctggagatcg ccgccctggt aggggtgatc ctggctgggg
     2581 cagcccaccg gcttccggtg gtgatagacg gcttcatctc gggtgccgcc gcgctggtgg
     2641 ccgtgcgcct ctgccctgcg gtccggccct acctggtgcc ttcgcaccaa tcggtggagg
     2701 tggggcaccg ggcgattctc cgggatctcg gacttagggc gctcttcgat ctggacctac
     2761 ggctcgggga gggaaccgga gcggtcctgg ccatgcacct gatagatgac gctctcgccc
     2821 tccacagcga gatggccacc tttgccgaag cgggaatcgg ggagtaggcg agaagcgagt
     2881 agcgaataga gaatagccaa tagagattag agagcagatg ctccagagaa catctattct
     2941 ctattcgcta cttgctcgcc cgcccttcgt cgctggagtt ctttcatctt ctgggaggca
     3001 gcggcgcggt tctggccgac cttggtagtg ttgcggaccg cccgatactg ctgaatcgcc
     3061 ttgtccagat cacccctacg ctcgtaggcc gtggccagca cgagatgggt caactcttcc
     3121 ttcttgttca tctccacagc ctgctccagg tgaggaagcg cctcctcgag gcgatttctc
     3181 tccagcagcg cgtttccata ggaaatgcgg gagcgccagt cgttaggtgc gacctcgcag
     3241 atgcgagcgt attccgcgag ggactcctct ttcttgccca tgtcgttgta gatcttcgcc
     3301 acggcgatct gggactcgat gtccgcgggg tctaggcgaa cagccttgcg catgaacttc
     3361 agcgcctggg cgagcttccg ctgggtaagg tagacggttc cgagattgtg gtgggctgcc
     3421 gcatggtcgg gggcaatttg gatcgcttcc tgtagcagct tttcggcctc gtccagatgg
     3481 ttatgggcaa tccggtcgat agccttcttg ttgagcgccg ttgccttctt gtactcgtcg
     3541 ctcggtccgg ttcggccgag tatgttttca agccagccca ttggacctcc agcatctggg
     3601 tatcgatcac ttctatccta tgacaggtcc ggtgtcaata ccgtcgagtt tacaggtgag
     3661 gcaggacgtt ctatgatgag cgccacctgt gacagggagg tcccaccttg gtttctggct
     3721 ccgccaggtc gcgctccatt ctcttctttt ccgtcgtcac caccctctac tggtcgtcca
     3781 tgtacatata cgtgcccacg ctctcggttc acgcggagaa cctgggggct tcggtggcgc
     3841 tggtgggcat gatcgtggcc tcctacggat tcccccagat cctggtgcgg attccgatgg
     3901 gcatctggtc tgatcggatc ggggtgcgga agccgttcat cttagctggc atcgccatct
     3961 caaccctggc ggggttgggg ctcgccctca cttctgatcc tgctttgctc gtagtctgga
     4021 gagcgatgag cggggtgggc gctcccgcat ttcctatgga aacgcgctgc tggagagaaa
     4081 tcgcctcgag gaggcgcttc ctcacctgga gcaggctgtg gagatgaaca agaaggaaga
     4141 gttgacccat ctcgtgctgg ccacggccta cgag
//
LOCUS       DATALS010000335         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_35915,
            whole genome shotgun sequence.
ACCESSION   DATALS010000335 DATALS010000000
VERSION     DATALS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35915"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VHS06_07535"
     CDS             complement(<1..192)
                     /locus_tag="VHS06_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcobamide--CoM methyltransferase"
                     /protein_id="HEX2988006.1"
                     /translation="MASEMTPTRRALAAVLGGRVDYVPPANPLAQTTVDLMKFANAEW
                     PKAHFDPKMMADLAAAPYEV"
     gene            complement(550..>1626)
                     /locus_tag="VHS06_07540"
     CDS             complement(550..>1626)
                     /locus_tag="VHS06_07540"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX2988007.1"
                     /translation="VGEQKTKPTQHSVKELRAIGIQPDVIVARACEPLEYSIKRKISL
                     FCDVSLEAVVSAPDAKSIYEIPIVLEEQGLTDFIVKRLGIDAQKKDLSTWREYLDRIL
                     YPSNTVRIAIVGKYTHLSDSYLSHLEAFHHAGAELGTRIECRFFDSEKVEKDGISQEL
                     ACCDAILIPGGFGSRGIEGKVMTAKLAREKKIPYLGVCLGFQIATIEIARDMLGMKGA
                     HTTELDPNTEYPVVDLLPEQRGVKDMGGSMRLGSQKVLVKEGSKAATLYGTTEIAERH
                     RHRYEVNPEFIERLEEAGWEFTGRSEDGIKMEIGELKGHPFFVACQFHPEFKSRPKKP
                     SPLHVGLVKAALEYKQARADRNVL"
BASE COUNT          285 a          495 c          490 g          356 t
ORIGIN      
        1 cacctcgtag ggggccgcgg caaggtcggc catcatcttg gggtcgaagt gggccttggg
       61 ccactcagcg ttcgcaaact tcatcaggtc cactgtcgtc tgggcgagag gattcgcggg
      121 cgggacatag tccacccttc cccctagaac ggcagcgagt gctctgcgag tcggtgtcat
      181 ttcgctagcc actaaatacc tcctgttgac ccggacacgg gataggcaaa ggcaccgtgc
      241 gtggtcgctg cattactgcc ttggtctcta taagcaatta taggcggatg gataatccat
      301 ccatttaatg ccgtttccgt tttcttgatc gacaacccca agatggcgta ggatgccgtg
      361 ctgggcggtt caatgaccct cgatcgttaa ggcctggctc tggacgaatt catggcggcg
      421 agaattcgac cgttctgtga gcgacgcgat cgagatggat gccgatcagt ggatattggg
      481 tctgcttcgg caaggggcgg cgatcgaggc agcggagcgg aatatggaaa agaggatgaa
      541 gaaagggttt cagaggacgt tccggtccgc cctggcctgc ttgtattcca gggccgcctt
      601 gaccaggccc acatgcaggg gggagggctt cttcggccgg gacttgaact ccggatggaa
      661 ctggcaggcc acgaagaacg gatgcccctt cagctccccg atctccattt tgatgccgtc
      721 ctcggaacgg ccggtgaact cccagcccgc ctcctcgagc ctctcgatga actcggggtt
      781 cacctcgtag cggtggcggt ggcgctcagc gatctcggtg gtgccgtaca gcgttgccgc
      841 cttggagcct tccttgacca gcaccttctg ggagccgagc ctcatggagc cgcccatgtc
      901 cttcactccc cgctgctccg gcagcaggtc gaccactggg tactcggtgt tggggtccag
      961 ctcggtggtg tgcgcgccct tcattcccag catgtcgcgg gcgatctcga tggtggcgat
     1021 ctggaatccc aggcagacgc ctaggtaggg gattttcttc tcccgtgcca gcttggccgt
     1081 catgaccttc ccctctatgc cccggctccc gaacccgccg gggatgagga tggcgtcgca
     1141 gcatgcgagt tcctgggaga tcccatcctt ctccaccttc tctgaatcga agaaccggca
     1201 ctctatcctt gtgcccagtt ccgccccggc gtggtggaac gcctccaggt gagacaggta
     1261 gctgtcagag aggtgggtat acttcccgac gatggcgatt ctcacggtgt tcgaaggata
     1321 caggatccgg tcgaggtact ccctccacgt gctgaggtcc ttcttctggg cgtcgatgcc
     1381 cagcctcttc acgataaagt cggtgaggcc ctgctcctcg aggacgatcg ggatctcgta
     1441 gatcgacttg gcatcgggcg ccgagaccac cgcctccagc gacacatcgc agaacaggga
     1501 gatctttctc tttatggagt actccagcgg ctcgcacgcc ctcgccacga tcacatctgg
     1561 ctggataccg atggccctta gctccttcac cgaatgctgg gtcggcttgg tcttctgctc
     1621 ccccac
//
LOCUS       DATALS010000336         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_219317, whole genome shotgun sequence.
ACCESSION   DATALS010000336 DATALS010000000
VERSION     DATALS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219317"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..199)
                     /locus_tag="VHS06_07545"
                     /pseudo
     CDS             complement(1..199)
                     /locus_tag="VHS06_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
     gene            171..1676
                     /locus_tag="VHS06_07550"
     CDS             171..1676
                     /locus_tag="VHS06_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2988008.1"
                     /translation="MSAPSTSTGHVNSDRRQALEASLDGYSAIALPRPATEEETQRLV
                     EAFLKGLRKLLSKDSNWAFWQPLQLSLDNCTRCQSCSEACHIYEAGGGQEPYRPSFRA
                     ELLRRIIRKYLTPGGKLRVKLTGTDIELTWTAIARLAELAYRCNLCRRCAQACPIGVD
                     NGLIAHEIRKLFSMEMGIAPKEIHQQGTVNQLNSGSSTGMTPAALKDNVEFLEEEVQE
                     RTGMEFKWPVDVEGADILLLHNAGEYMAWPENPEAFAIILEKAGISYTLSSDLMGYDA
                     VNYGLWYDDVQFARIALKHAQVAKKLGVRKIVIGECGHAHKALSVIADRIFGVEYNVP
                     RESCLTLLDGIVAGGRLQLDPSRNDFPVTLHDPCNVVRLMGIVEPQRRILRKIAPRFR
                     EMTPHGVDNYCCGGGSGFAIMNGLNFPEWRNSISGRKKLQQILNAFHGELDRSIPKYV
                     CAPCSNCKGQIRELLAFHGVDSGCSIKYGGLVELIVNAMPDIQRPFISWEG"
     gene            complement(1739..1927)
                     /locus_tag="VHS06_07555"
     CDS             complement(1739..1927)
                     /locus_tag="VHS06_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2988009.1"
                     /translation="MYIVTVDEEKCEGDGECVNICPVSMFELVGGKAAVTGNQDDCLG
                     CESCVAVCPSGAITVTEM"
     gene            complement(1973..2290)
                     /locus_tag="VHS06_07560"
     CDS             complement(1973..2290)
                     /locus_tag="VHS06_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEX2988010.1"
                     /translation="MPTVKLGGRQFDVDEDGFLEDPTIWNEEVVSCFAEAESISELTP
                     DHWKVINYLRDYYSQFGIAPMIRKLCKDTGYDLKTIYQLFPSGPAKGACKLAGLPKPT
                     GCV"
     gene            complement(2366..>2877)
                     /locus_tag="VHS06_07565"
     CDS             complement(2366..>2877)
                     /locus_tag="VHS06_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="menaquinol oxidoreductase"
                     /protein_id="HEX2988011.1"
                     /translation="LCFAVRRFTGFDPGAKAIQKLGLIVTYAMIVNLFLVVIEVVVAV
                     YSGVPAHIDHFRYLYLGLDGRGALVPWMWVSTILGFGAVVALLFQRVRLNERWLAMAC
                     AAVFISTWIDKGLGLVVGGFVPSTLGRITEYLPTAAELSISVGVYALGALILTLLYRI
                     TMSVKEETG"
BASE COUNT          605 a          909 c          849 g          514 t
ORIGIN      
        1 cgattcgggc gaactggacg tcgtcgtacc acaatccgta gttcacggcg tcgtagccca
       61 tcaggtcgct ggagagggtg tagctgatgc cggctttctc cagaatgatg gcgaacgcct
      121 ccgggttctc gggccaagcc atgtactccc ctgcgttgtg gagaagcagg atgtcggctc
      181 cctcgacgtc caccggccac gtgaactccg atcgccggca ggcactggaa gcatccctgg
      241 atgggtactc ggccatcgct ctcccaaggc ctgccacgga ggaggagacg cagaggctgg
      301 tcgaggcgtt cctgaagggg ctgaggaagc ttctctccaa agatagcaac tgggccttct
      361 ggcagccgct ccagctctca ctggacaact gcacccgctg tcagagctgc tcagaggcat
      421 gccacatcta cgaggccggc ggcgggcagg agccctaccg gcccagcttc agggccgagt
      481 tgctgcgacg gataatccgc aagtacctca cgccgggggg aaagctccgg gtgaagctga
      541 cgggcaccga catcgagctg acctggactg ccatcgctcg gctggcagag ctcgcatacc
      601 gctgcaacct ctgccggcgt tgcgcccagg cgtgtccgat cggagtggat aacggcctca
      661 tcgcccacga gatccgcaag ctgttcagca tggagatggg gatcgcgccg aaggagatcc
      721 accagcaggg caccgtgaac caactcaaca gcggatccag cacggggatg accccggccg
      781 cgttgaagga taacgtcgag ttcctggaag aagaggtcca ggagcggacg gggatggagt
      841 tcaagtggcc ggtggacgtc gagggagccg acatcctgct tctccacaac gcaggggagt
      901 acatggcttg gcccgagaac ccggaggcgt tcgccatcat tctggagaaa gctggcatca
      961 gttacaccct ctccagcgac ctgatgggct acgacgccgt gaactacgga ttgtggtacg
     1021 acgacgtcca gttcgcccga atcgccctca agcacgccca ggtcgccaag aagctagggg
     1081 taaggaagat cgtcatcggg gaatgcgggc acgcccacaa ggcgctctcc gtcatcgcgg
     1141 atcgcatctt cggtgtcgag tacaacgtcc ccagggagag ttgtctgacg ctgctggacg
     1201 gcatcgtcgc cggcgggagg cttcagttgg acccctcgcg gaacgacttc ccagtgacac
     1261 tccacgaccc ctgcaacgtg gtgcggctga tggggatagt ggaaccgcag cgaaggatcc
     1321 tgaggaagat cgccccccgc ttccgcgaga tgacccccca cggagtggac aactactgct
     1381 gcgggggagg aagcggcttc gccatcatga acggtctgaa cttccctgaa tggcgcaact
     1441 ccatctccgg ccgcaagaag ctgcagcaga tcctgaacgc cttccacggt gaactggacc
     1501 ggtcgatacc caagtacgtc tgcgcaccct gctcgaactg caagggccag atccgcgagc
     1561 tactcgcatt ccacggtgtg gactcgggct gcagcatcaa gtacggcggc ctggtggagt
     1621 tgatcgtcaa cgccatgccg gacatccagc gaccgttcat ctcctgggag gggtaagtga
     1681 agtgcgcggc ggggcgatcc accccatcgc cccgccgcgc atcagaagga agaaccgcct
     1741 acatctccgt aacggttatg gctccgctcg ggcagacggc tacacagctc tcacacccca
     1801 gacagtcgtc ctgattgccg gtgaccgcgg ccttgccccc caccaactcg aacatggata
     1861 cggggcagat gttcacgcac tcaccgtcgc cttcgcactt ctcctcgtcg accgtgacaa
     1921 tatacatccc tagattactt ccttcctagc gacgtggcgc agattatgtc gtctaaacgc
     1981 agccggtagg ttttggcagc cccgccagtt tgcaggcacc cttcgccggc ccggacggga
     2041 agagttggta gatggtcttg agatcgtagc cggtatcctt gcacagcttc ctgatcattg
     2101 gcgcgatgcc gaactgggag tagtagtctc gcaggtagtt gatcaccttc cagtgatctg
     2161 gggtgagctc cgagatgctc tcagcctcgg cgaaacagct aacgacctct tcgttccaga
     2221 tggtgggatc ctccaggaac ccgtcctcgt cgacatcgaa ttgtcgcccg cccagtttaa
     2281 ccgttggcat cacacacctc cttcacgagc tacatgatag tggatgttcg agctgagtca
     2341 agcatcgact acgtcgatgc ttgactcagc cggtttcttc cttcacggac atggtaatcc
     2401 gatagagcag ggtgaggatc agcgctccca gggcgtacac ccccaccgag atgctcagct
     2461 ccgcggcggt gggtaaatac tcggttatcc tccccagcgt agagggcacg aatccaccga
     2521 cgaccaaccc caatcccttg tcgatccacg tcgagatgaa cacggccgca caggccatgg
     2581 cgagccaacg ctcgttcagc ctcaccctct gaaacaagag ggcgaccacc gccccgaacc
     2641 cgaggatcgt ggagacccac atccatggca caagcgcccc tcgcccatcc agtcccagat
     2701 agagatagcg aaagtggtcg atgtgagccg gaaccccgct gtacaccgcc actaccacct
     2761 cgatcaccac caggaacagg ttgacgatca ttgcgtaagt cacgatcaat cccagtttct
     2821 ggatagcttt cgctccggga tcgaagccgg tgaagcgtcg gaccgcgaag cagagga
//
LOCUS       DATALS010000337         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_260052, whole genome shotgun sequence.
ACCESSION   DATALS010000337 DATALS010000000
VERSION     DATALS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260052"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VHS06_07570"
     CDS             <1..334
                     /locus_tag="VHS06_07570"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX2988012.1"
                     /translation="EILKGLELRARGPVLTSCPTCGRTEVDLLKLTAQVEEVLASVKT
                     ELHVAVMGCVVNGPGEAAEADLAVVGGKGVVALYRKGRFLRRVPEAQARSALLEEIQA
                     WEQDNTHA"
     gene            complement(502..1053)
                     /locus_tag="VHS06_07575"
                     /pseudo
     CDS             complement(502..1053)
                     /locus_tag="VHS06_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            complement(1284..>2389)
                     /locus_tag="VHS06_07580"
     CDS             complement(1284..>2389)
                     /locus_tag="VHS06_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2988013.1"
                     /translation="HTLQGGLEYFSVGVGPKVPVPDIVATALSVVGFPAMPLLDRMRM
                     GVVSAYLKYLVRDWRPLEQSTAVEWTRRWMGEKAYEAFVYPLLEGKFGRYAEQVNMSW
                     LWARLKTRSFRLGYFQGGFQGFVDALVAYVRREGAKVVLKTPISALEELPEGGWRVTS
                     GRRDDPSAISSEEFDSVIATGSPQLLKKLAPHLPSDYLGQIQKLDSLGAVVLTIALRH
                     SLTNGVYWMNMPKDEFPFLALVEHTNLMDSKHYGGDHLVYCGDYLEPSHEYFQMTKEQ
                     LLERFLPALKRVNPAFDRSWIRDSWLHREVYAQPIVPVNHSRNIPSLATPLKGLYWAS
                     MSQVYPWDRGTNFAVELGQRVARELSGSSGRRG"
BASE COUNT          460 a          792 c          725 g          412 t
ORIGIN      
        1 cgaaatcctg aaggggctgg aactgcgagc ccgaggtccg gtgctgacct cctgtcccac
       61 ctgcggccgc acagaggtag acctgctgaa gctgactgcc caggtggagg aagtcctggc
      121 atcggtgaag accgagcttc acgtggcggt gatgggctgc gtggtaaacg gtcctgggga
      181 agccgccgag gcagacctgg ctgtggtggg tggcaaggga gtcgtggccc tctaccgcaa
      241 ggggcgtttc ctccgccgcg tgccggaggc gcaagctcgg tccgcattgc tggaggagat
      301 ccaggcctgg gagcaggaca acactcacgc ctgagggctg atgcgattca gccaagcgtc
      361 cggacatggt gtgacaactt cgtagggcgg ggtcttgtgc cccgcctccc acaccatggc
      421 tacgaccacc ataccgtctt ttgccaaccc accaggcagg gcacaagacc ccaccctacg
      481 accacttgtg gctgacctca cctacctcgg ccgcttcacc tcgacgacgt agaggcgtcg
      541 atccaccatc tccaccagat cgtcgaagaa ctccaccgca tcctcggccg gcggtgcagg
      601 ctgaagcttc aggtaggccg ccgaccgacc gttgaacacc agcgtcggcg tcccccagat
      661 gccgtgcttc tgtaccccca gctcgtagtc gtcgccgatc ctggtcagca gaggccgatc
      721 ggcgagatcg tgctggaagc ggttcacgtc cagtcctgcc tccctggcca gatccgagag
      781 cactgcacga tcggagatgt ctcgcctctc ctcgtgccgc gcacggaaca gcgccatgtg
      841 gagatgctcg aaggcctccg ctccctggac tctggcagcc tcggccgcgc ggaaggccca
      901 caggcccctg ctgcggtagt cgtccggctg ctcccacagc ttccagtcgg gcccctggat
      961 gctgttgacc tgctccagtg ggaagctatg ccactccagc gtaagctttt cgcggtagat
     1021 ccgcttgacc caagacagcc agaccgctgc ctgataggca taaggtcagg tgtagtcggc
     1081 ccagacctcc agcgtgatct gctctgccat ctcccctcca caagaaaccg gcgtcgatgc
     1141 aacgcaccca cgacgccggg cacctattgc acccggttcc gaagaccagg tgtcaaatcc
     1201 tcggatcgac cgactgccac aggtggtagg ggcggacctg agtgtccgcc cctaccgatg
     1261 gcgcatcggt tgtcgacgaa ctgctagccc cgcctcccgc tggatcccga taattctcga
     1321 gccacccgct gccccagttc aactgcgaag ttcgtgcccc gatcccaggg gtatacctgg
     1381 ctcatgctgg cccagtacag ccccttcaag ggagtggcaa ggctgggaat gttgcgggag
     1441 tggttcaccg gaacgatggg ctgcgcatac acctcgcggt gcagccagga gtcccgtatc
     1501 caggatcgat cgaacgcagg gttcacccgc ttgagggccg gcaggaaacg ctccagcaac
     1561 tgctccttcg tcatctggaa gtattcgtga gagggctcca ggtagtcacc acagtagacc
     1621 agatgatccc ctccgtagtg cttcgagtcc atcaggttag tgtgctcgac caacgccagg
     1681 aaggggaact cgtccttcgg catattcatc cagtagacgc cgttggtcag ggagtggcgc
     1741 agcgcaatgg tgagcaccac cgcgccgagg gaatccagct tctggatctg ccccaggtag
     1801 tcggagggga gatggggcgc aagcttcttc aacaactgcg gggacccggt cgcgatgacg
     1861 gagtcgaact cctcggagga aatcgcggag ggatcatccc gacggccgga ggtgactcgc
     1921 cagcctcctt caggtagctc ctccagggcg gaaatcggcg tcttcagcac gaccttagcc
     1981 ccctcacgcc gaacgtacgc cacgagggca tcgacgaagc cctggaagcc cccttggaag
     2041 tagccgagcc ggaaggacct ggtcttcagc cgcgcccaca gccaagacat gttcacctgc
     2101 tccgcgtacc ggccgaactt cccctctaga agggggtaga cgaaggcttc ataggctttc
     2161 tcccccatcc agcggcgggt ccactccacg gccgtggact gctccagcgg tcgccaatcg
     2221 cgcaccaagt acttcaagta ggccgagacg acgcccatcc gcatgcgatc caggaggggc
     2281 atggcgggga atccaaccac tgagagggcg gtagccacga tatccggcac agggaccttc
     2341 ggtccaacgc cgacggagaa gtactccagc cccccttgca gcgtgtgtg
//
LOCUS       DATALS010000338         3716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_170317, whole genome shotgun sequence.
ACCESSION   DATALS010000338 DATALS010000000
VERSION     DATALS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170317"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..672)
                     /locus_tag="VHS06_07585"
     CDS             complement(187..672)
                     /locus_tag="VHS06_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988014.1"
                     /translation="MDQLKIWGERVGLPVVAHQPGADPGSVVFDGLTAAKSRGADLVV
                     IDTAGRLHTKFNLMEEMKKIKRIVEKQNPDHMATVLVLDATTGQNALIQAKQFQEAVD
                     LDAVVVAKLDGTAKGGIVFALADQLNVPICFVGTGETLDDIAEFDPDRFVDSLLSQAA
                     E"
     gene            969..2198
                     /locus_tag="VHS06_07590"
     CDS             969..2198
                     /locus_tag="VHS06_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2988015.1"
                     /translation="MAELRVLEPPAVATKPRITYMASYPPRECGIATFTKDLVDAVDR
                     TFPGHPAKIIALNESQATYQYGQEVKVAVTVDDPESYREAADYVNRSRTDLLCIQHEY
                     GLFGGQHGENIFTFLDALRKPLAITLHTVLPRPDDHLREVTREIVSRADRTVVLAKKA
                     LDILAEDYGISAPSVRYVPHGVPNVRKLPSTSFKSILGLEGRFTVSTFGLINPGKGIE
                     YAIKALPEVVKKYPSVLYLVLGETHPGVRKNEGENYRNCLHDLVSELGLKKHVKFNNR
                     YLTHNEIVSYLMATNIYLVPYLNPNQIASGTLAYAVGAGRAVVSTPFIYATELLEDGR
                     GILVPFRDSDAIAEALDGLIGDAAGRSSMAMAAYAYGRNMTWHSVAREYMQLFGDLVG
                     RPTPMPMASNNSRALAL"
     gene            2237..3409
                     /locus_tag="VHS06_07595"
     CDS             2237..3409
                     /locus_tag="VHS06_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988016.1"
                     /translation="MERVPGFKLDRLKRMTDDTGVVQHSLYAIPDHNYGYSIDDQARA
                     LIVALKHFNLTGNWESVDLATCYLRFLNYAQLPDGRFHNFMAFDRHWQDEVGSEDSNG
                     RVVWALGYTALTGVEEDLQMTARTMLLRAFPAIRKLSYLRARAFAILGLYHYLRTEPG
                     DQQAREMVLLHADALLRAYRSESGSDWDWFEDRLTYSNGRLPQSLMLAYLITWKPEYL
                     EVARHSLDFLLRTAHLFEPDSIDLVGQDGWYTRGASCARYDQQPVDANGLLDACLTAH
                     QLLADKRYLSEAQLAFAWYHGRNVLGKPLYCESTGGCFDGLTPDGMNRNQGAESTISY
                     LLSYLALVESGHLALNEATREKRRPQLIVPRLVPLQPVGEEPSTVQTPSVIIRGTQ"
     gene            3523..>3716
                     /locus_tag="VHS06_07600"
     CDS             3523..>3716
                     /locus_tag="VHS06_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX2988017.1"
                     /translation="MFESLSDKLQAVFSRLRNKGKLTEEDVDLALKEVRLALLEADVN
                     FKVVKGFVAKLRERAIGSEVL"
BASE COUNT          761 a         1116 c         1109 g          730 t
ORIGIN      
        1 ctgtcaggat cactgtggtc gaggaccagt gccctgtcac agccgaatcg acgagtagta
       61 ggggcgaacc tatgtgtccg ccttcggacc tgggcgcaca cgcagcgtga cgcgagcgag
      121 cacagcgtca cggccccttc cattcatcag atgccgtgtg acagagcact ggaacgaaaa
      181 gcggggctac tcggcggcct gagacagcag cgagtccacg aagcggtctg gatcgaactc
      241 cgctatgtca tccagcgtct cgccggtgcc cacgaagcag atggggacat tgagctgatc
      301 ggcaagggca aagacgatgc cgcccttggc ggtgccgtcc aacttcgcca ccaccacggc
      361 atccaggtcc acagcctcct ggaattgctt cgcctggatc agcgcgttct gcccggtggt
      421 ggcgtccagc accagcaccg tggccatgtg atccgggttc tgcttctcga cgatccgctt
      481 gatcttcttc atctcttcca tgaggttgaa cttggtgtgc aatcggccgg cggtatcgat
      541 caccaccagg tcggctcctc gcgacttcgc cgcggtgagt ccatcgaaga cgaccgaacc
      601 cggatccgct ccgggctggt gagcgactac aggcagcccc acccgctcgc cccagatctt
      661 cagttggtcg attgccgccg ccctttctca gagatagccc ccctgaatta ggctgtgatc
      721 aactatttgc taattctaca gcaaagcatg taagctagtg taacgaggca tgtagtacgg
      781 cgcgatttat gcttgagaga aaccaaggcg tatcaccgca gcgtggcttc acctgagtaa
      841 gacggtagag gccacgggag cgttttccgc ctcggcccct agccccggtc tggatgcatc
      901 ggtattcgcg gggttcatcg ccatgtgagc aggccgagcg cctgcaatag ctcgctgagg
      961 aggaacatat ggcagaactg cgggtcctgg aaccacctgc ggtagccacg aagccccgca
     1021 tcacatacat ggccagctat ccgccccgcg aatgcgggat agccaccttc accaaggatc
     1081 tggtggacgc tgtcgataga actttccccg ggcatcccgc caaaatcatt gcgctgaacg
     1141 agagccaggc gacctaccag tatggccagg aggtcaaggt cgccgtgacc gtggatgacc
     1201 cggagagtta ccgtgaagcc gccgactacg tgaaccggtc tcggacggat cttctctgca
     1261 tacagcacga gtacggactg tttggcggac agcatgggga gaacatcttc acgtttctgg
     1321 acgcccttcg aaagccgctg gctattactc tccacacagt gttgcctcgg cccgacgacc
     1381 atctgcgtga ggtaaccagg gagatagtat ccagggccga ccggaccgtg gtgctggcca
     1441 agaaggcgtt ggatatcctt gctgaggact acggcatcag cgctccctca gtgcgctacg
     1501 tccctcacgg ggtgcccaac gtgcgcaagc tcccgtccac cagttttaag tccattctag
     1561 ggcttgaagg acgcttcacc gtctcgacgt tcggcctgat caaccccggc aaggggatcg
     1621 agtatgccat caaggctttg cctgaggtgg tgaagaagta tcccagtgtc ctgtacctcg
     1681 tattgggcga gacccatccg ggtgtcagaa agaacgaggg cgagaactac aggaactgcc
     1741 tgcacgacct cgtgtccgag ttggggctga agaagcatgt gaagttcaac aataggtatc
     1801 tcacgcacaa cgagatcgtg agctacctca tggccaccaa catctacctg gtcccatacc
     1861 tgaaccccaa ccagatagcc agtgggactc tggcgtacgc ggttggcgcg ggacgagcgg
     1921 tggtttccac gccattcatc tacgccacag agctgctgga ggatgggagg ggcatattgg
     1981 tgcccttcag ggattccgat gccattgccg aagcgctgga tgggctgata ggcgacgctg
     2041 caggccgttc ttccatggcg atggcggcct acgcatacgg gcgaaacatg acctggcaca
     2101 gtgtcgctag agagtacatg cagcttttcg gcgacttagt gggacggccg accccaatgc
     2161 cgatggcatc caacaatagc agggcactgg ccttgtaggg ggcactccga atgtcagggg
     2221 taaatgagac atggcgatgg aaagggttcc cggcttcaag ctggacaggc tgaagcggat
     2281 gaccgatgac acaggcgtgg tgcagcactc gctctacgcg attccggatc acaactacgg
     2341 ctactccatc gacgatcagg ctcgggcgct catcgttgcc ctaaagcact tcaacctcac
     2401 cggtaactgg gagagcgtgg accttgccac atgctacctg cgattcctga actacgccca
     2461 gcttcccgat gggcgattcc acaacttcat ggcctttgat cgtcactggc aggacgaggt
     2521 gggctctgag gactctaacg ggagggttgt ctgggcgctg ggctacaccg cccttacagg
     2581 ggtggaggaa gatctccaga tgacggccag gaccatgctg ctgcgcgcct tccctgccat
     2641 tcggaagctg agctacctga gggcacgggc gttcgccatt ctcggtctgt accactacct
     2701 tcggactgag cccggtgacc agcaggccag ggagatggtc ctgctccacg ccgatgcact
     2761 actgcgagcc tataggagtg agtccggctc cgactgggat tggttcgagg atcgcctgac
     2821 ctacagcaac ggccggctcc ctcagtcgct tatgctcgcc tacctcatca cctggaagcc
     2881 cgaatacctc gaagtggcga gacactcgct ggacttcctt ctccgcacgg ctcatctatt
     2941 cgagccggat tccatcgatc tggtcgggca ggatgggtgg tacactagag gtgcttcctg
     3001 cgcccgctac gaccagcagc cggtggatgc caacgggctg ctggatgcct gcctgaccgc
     3061 acaccagttg ctggccgaca agcgatacct gagcgaggct caattggcct ttgcctggta
     3121 ccatggcagg aatgtcctgg gcaagccgct ctactgcgag agcacagggg gctgcttcga
     3181 tgggctgaca ccagatggca tgaaccggaa ccagggtgcg gagtcgacca tctcctacct
     3241 gctctcctac ctggccctgg tggagtccgg gcacctcgcg ttgaacgaag ccacgcgaga
     3301 gaagcgccgt ccgcaactga tcgttccgcg attggttccc ctccagcctg taggcgagga
     3361 gccctcgact gtccaaaccc cgtcggttat cattagaggt acacaatagg cttcccccaa
     3421 aggggaacgc atgcgcgggc gacctgaccc acatgccccc tcgactcatt gtgtcgtggc
     3481 cggttgggcc ggtactagca gtaaccgcga ggtgagaatc cagtgtttga aagcctttcc
     3541 gacaagttgc aggccgtttt ctcacggctt cgaaacaaag gcaagctgac cgaagaggac
     3601 gtggaccttg cccttaagga ggttcggctc gccctgcttg aggctgacgt caacttcaag
     3661 gtcgtcaagg gcttcgttgc caagctgcgc gagcgggcta tcgggagtga ggtgct
//
LOCUS       DATALS010000339         4613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_36386,
            whole genome shotgun sequence.
ACCESSION   DATALS010000339 DATALS010000000
VERSION     DATALS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36386"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1566)
                     /gene="pyk"
                     /locus_tag="VHS06_07605"
     CDS             complement(142..1566)
                     /gene="pyk"
                     /locus_tag="VHS06_07605"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX2988018.1"
                     /translation="MRRTKIVCTLGPASSNEEVIRALIRAGMDVARLNFSHGTHETHA
                     GLIATLRRVAAEEGRHTAILQDLQGPRIRIGDMDGGQATLIQGDQFALTTQEVSGNPE
                     KATIRGAPLPQDVHTGDRLLLDDGNLELVVERTTPTDVICKVVVGGILKSHKGVNVPG
                     RTLSVPSLTEKDQEDLVFGVEQGVDYVAMSFVRSGEDVLRLKALLAKHGAETPVIAKI
                     EKHEAIANFDEILKASDGIMVARGDLGIEVAAEEVPLHQKMIIHKCNAAGKPVITATQ
                     MLDSMIRNPRPTRAEVNDVANAILDGSDATMLSGETAAGAYPLEAVATMARIAETTDE
                     ALPYDRLMRRTSANRMCNVTDAIGQATCEMAYELKAAAIITSTESGFTARMVARHRPP
                     VRIIAATPSERTLRQLALVWGVHPHLTDSFNSTEDMIRQVVTLAMHEESVIEGDLVVI
                     TAGLPLMAKGRTNLLKLHVVGEPV"
     gene            complement(1657..2613)
                     /locus_tag="VHS06_07610"
     CDS             complement(1657..2613)
                     /locus_tag="VHS06_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2988019.1"
                     /translation="MRALLIHNTRSGQRDRDGEVAAAVQRLVSAGWKLDVVSSASAAE
                     LESQARRAVDAGVDMVIAAGGDGTLNLAIQALAYRRAVLGVLPTGTTNVWAQELGIPT
                     DIPAATQLLLSGCTARVDLGRADGRYFLFVAGIGFDASVTREISPAAKRKLGKLAYVV
                     AAIVQAAKLRGTEATIVADGKVSRQRVLMVVASNTRLYGGVLNMAPEAFVDDGLLDFW
                     VFRGRGLMAAAVHALTILVGRHYQDPGARYYRSASLTVISRGSLPVQLDGDYYGVTPV
                     TISVAPEALRVVVPPGHHAILSRQLESWNPTTTESSRRPRRY"
     gene            complement(2610..3170)
                     /locus_tag="VHS06_07615"
     CDS             complement(2610..3170)
                     /locus_tag="VHS06_07615"
                     /inference="COORDINATES: protein motif:HMM:NF020022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988020.1"
                     /translation="MSHEAVPNRPDDNEGPALVIMAGPPGTGKSHLVRLVRQRLPVMV
                     VESDAIRHRIAQHPTYTPEESRHVFYLAHRRIDRLLARGKMVVFDATNIYEWGRRTVY
                     RIAERRGARVLVVRTVAPDEVVAQRLEARLAGANPADRSEAGWEVYAKMKKRFQEIDR
                     PHLVVDTSAELSPALDRIVTFLGGAD"
     gene            3362..4273
                     /locus_tag="VHS06_07620"
     CDS             3362..4273
                     /locus_tag="VHS06_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX2988021.1"
                     /translation="MTDLVLNDRYRLIAPLGEGGMAVVYEANDLLLDRKVAVKLLRPQ
                     YATDPAFLARFQREAKAAARLSHPNVVAIYDVGSEGDTQYIVMELVEGRTLKELVEAE
                     GPLAAAHIIDLGRQICEALDYAHRHQIVHRDVKSQNILVTGDGRAKIADFGIAVALGA
                     SSLTQAGFVVGSAHYFSPEQAQGEPTTPYSDIPINRGTITFGAPLLTESATSEPFSAF
                     DSATGFWDRTVPAGTRSLASSVVFQVKPAACSFPCATDNPSPTTRGTEANSLPWLSTT
                     SILLPFSIWVPADGFWETTDLAGYLES"
BASE COUNT          785 a         1504 c         1393 g          931 t
ORIGIN      
        1 caccgtggtg gtcgcggccc ggatggggga ggcggggcac acgaccccgc cctacgagaa
       61 ttgcgacgct gtggctctac ctgtgactgg tccgcgtcag aggtaagagc atgccccaaa
      121 gatgcggccc gtgccctcgc ctcacaccgg ctcgcccacg acgtgcagct tgaggagatt
      181 cgtgcgcccc tttgccataa gcggcagacc ggcggtgatc actactaggt cgccctcgat
      241 cacgctttcc tcgtgcatgg ccagcgtcac cacctgccgg atcatgtcct ccgtcgagtt
      301 aaagctgtca gtaaggtgag ggtgtactcc ccacaccaac gccaactgcc ggagcgttcg
      361 ttctgaaggg gtggcggcga tgatccttac cggcggtcga tggcgagcca ccatccttgc
      421 cgtgaatccc gactccgtag aggtgatgat ggccgccgcc ttcagttcgt aagccatctc
      481 acacgtggcc tgtccgatgg cgtcggtaac gttgcacatc ctgttggcgg aggtgcggcg
      541 catcaacctg tcgtagggca acgcctcgtc ggtggtctcg gcgatccgtg ccattgtggc
      601 aaccgcctcc agcgggtaag cccccgccgc agtttccccg gagagcatcg tcgcgtcgct
      661 cccgtccaag atagcgttgg cgacgtcgtt gacctctgcc cgcgttggcc ttgggttcct
      721 gatcatggag tccagcatct gggttgccgt gatcaccggc ttgcccgccg cgttgcattt
      781 gtggatgatc atcttttgat gaagcggcac ctcttcggcg gccacttcga tgcccagatc
      841 gccgcgtgcc accatgatgc cgtcgctggc tttcaatatc tcgtcgaagt tggcgatggc
      901 ttcatgcttc tcgatcttgg caatcacagg cgtctctgcg ccatgtttcg caaggagagc
      961 cttcagccgc agcacgtcct cccccgatcg gacgaaggac atcgccacgt agtcgactcc
     1021 ctgctccacc ccgaagacca ggtcttcctg gtccttctct gtgagggagg ggacgctgag
     1081 ggtgcggccc ggcacgttta cacctttgtg ggacttcaga ataccgccca ccactacctt
     1141 gcagatgacg tcggtgggcg ttgtccgctc caccaccaac tccaggttgc catcgtccag
     1201 caggaggcgg tcgccggtgt ggacatcctg agggagcggg gcgccccgga tggtcgcctt
     1261 ctccgggttg ccgctcactt cctgcgtcgt caatgcgaat tggtcacctt ggatcagggt
     1321 tgcctggccc ccgtccatgt cgccgatcct gatcctcgga ccctgcaggt cctggaggat
     1381 tgccgtgtgc cggccctctt cagctgctac tcttcgcaac gtggcgatga ggccggcgtg
     1441 ggtctcgtgg gtgccatggg agaagttgag gcgagcgacg tccatgcccg cgcggatgag
     1501 ggcccggatc acctcctcgt tggaggaggc agggccaagg gtgcatacaa tcttggtgcg
     1561 gcgcaaggca acctccttgg gtcgagcaat cagccgtcag ctattgtgcc gtcagctata
     1621 aggtatcagg tatttgtgtc gacgtgggag cagggatcag tatcggcgtg gtctgcgcga
     1681 ggattcagtc gtggttgggt tccaggactc caattggcgt gagagtattg cgtggtgtcc
     1741 cggcggaacc accactcgca acgcctccgg ggccacactg atggtaaccg gtgttacgcc
     1801 gtagtaatca ccgtccagct gcacagggag actccccctg gatataaccg tcagggaagc
     1861 gcttcggtag tagcgagccc cggggtcctg gtagtggcgt ccaacaagta tggtaagggc
     1921 gtggactgcg gctgccatca atccacgccc ccgaaatacc cagaagtcca gaaggccgtc
     1981 gtcgacgaat gcctcggggg ccatattgag gacgccgccg tacagtcgag tgttgctggc
     2041 caccaccatc agcacacgtt gccggctgac cttgccgtcg gcaactatcg tggcctccgt
     2101 gcccctcaac ttcgcggcct ggacgatggc ggcgaccacg tacgccagtt ttcccagttt
     2161 gcgcttggcg gcggggctga tctcccgcgt gacggatgcg tcgaagccga tcccagccac
     2221 gaaaaggaag tagcgcccgt ctgccctgcc caggtccacc cgtgccgtgc accccgatag
     2281 tagcagttgg gtggcggcgg gaatgtcagt cgggatcccc aactcctgcg cccagacgtt
     2341 tgtggtaccc gtaggcagga ctcccagcac cgcccgtcga taggccaacg cctggattgc
     2401 caggttcagg gtgccgtcgc cgcctgccgc tatcaccatg tcgactccgg catccactgc
     2461 gcgtcgtgcc tgggattcca gttccgcggc ggaggctgag gataccacat ccagcttcca
     2521 gccggcagaa acgagcctct gcaccgcggc ggcgacctcg ccgtcgcgat ccctttgtcc
     2581 tgagcgggtg ttgtggatca gcagcgctct caatctgctc ccccgaggaa agtgacgatc
     2641 cgatccaatg ccggcgaaag ctccgccgag gtatccacca ccaggtgggg gcggtcgatc
     2701 tcctggaaac gtttcttcat cttggcgtac acttcccagc cggcctcgga tctgtctgcc
     2761 gggttcgcac cggccagccg agcttccagc cgctgcgcca ccacctcgtc cggtgccacg
     2821 gttcgcacca ccaggaccct ggcgccccgt cgttcggcta tacgatacac cgttcggcgt
     2881 ccccattcat atatgttggt ggcgtcgaag accaccatct tgcccctggc aaggagccga
     2941 tcgatgcggc ggtgtgcgag atagaagacg tgccggctct cctcaggcgt gtaggtagga
     3001 tgctgcgcga ttcgatgccg gatggcgtcg ctctccacca ccatcacggg tagccgttgc
     3061 ctgaccagtc ggacgagatg cgacttaccc gttccgggtg gcccggccat gattaccagt
     3121 gctggcccct cattgtcatc gggcctgttc ggaaccgctt catgcgacat gtctgccctc
     3181 tccacgttga tcctgcacca tccagtcgca gcctattcgc cgttcccagg cgatgatatc
     3241 aaacgcggtg cccggctcgc cttgtgtgtt cctacactct ttgtgtaaaa tgctcttaat
     3301 tatgcgccat gatgtctgtt agtctcccat tgactctcca agaagaaacc ccatgagttc
     3361 aatgacagat cttgtcctca acgatagata caggctgatt gcccctttgg gcgagggggg
     3421 gatggccgtt gtctatgagg ccaacgacct tctgctggat cgaaaggtcg cggtgaagct
     3481 gcttcgcccg cagtatgcta ctgatcccgc cttcctcgcg cgcttccagc gcgaggccaa
     3541 ggccgccgcc cgactctccc accccaacgt ggtggcaatc tacgacgttg gctccgaggg
     3601 cgacactcag tatatcgtca tggagttggt ggagggacgc accctgaagg agttggtgga
     3661 ggcggagggg cctctggccg ccgcccacat catcgacctg ggtcgccaga tctgcgaggc
     3721 gctcgattac gcccatcggc accagatcgt ccacagggac gtgaaatccc agaacatcct
     3781 ggttaccggg gatggcaggg ccaagatcgc cgatttcggg atcgcggttg ccctgggcgc
     3841 ctcctccctc actcaggcgg gcttcgtggt gggctcagcg cactacttct cgccggagca
     3901 ggcccaggga gagccaacta ccccctactc ggacatccca atcaacaggg gcactatcac
     3961 ctttggcgcc ccactgctca cggagagcgc cacttcggaa cccttctctg ctttcgactc
     4021 ggccacagga ttctgggaca gaacggtgcc tgcgggcaca cggtcgctcg cctcatcggt
     4081 tgtcttccag gtgaaaccgg cagcctgcag cttcccctgc gccacagaca acccctctcc
     4141 aaccacccgg ggcaccgagg ccaactcctt gccctggctg agcaccacct cgattttgct
     4201 ccccttctcg atatgggtgc ctgcggatgg attctgggag accacggacc tggccgggta
     4261 cttagagtcg taggcttctc cggtgatgac caactcgaaa ccggcagcct gtgcctgctg
     4321 caaagccgtc tggtacggga tcccgaccaa ttgtggtgtt tgcagcggcg aggggctcgg
     4381 ggtgacctgg ggcgtcgcaa cctttgtcgc ctgcggagtg gggctctgac cggtgcccgg
     4441 cgtgcgggtg gtctcgggcg atatggcggc cgccccctga tcgagccacc ccagcatacc
     4501 gttggagtat gcgagaatcc ccagtggcac cgctcccagc gtgcagagga acgccgcgaa
     4561 caccaccaga agaaccgtcc acccaccgga accgcgcttc cgcgcccgag gtt
//
LOCUS       DATALS010000340         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_36488,
            whole genome shotgun sequence.
ACCESSION   DATALS010000340 DATALS010000000
VERSION     DATALS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36488"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VHS06_07625"
     CDS             <1..1179
                     /locus_tag="VHS06_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX2988022.1"
                     /translation="RMEKENSKYSLLNKEGRLPNAMAIKEAAAKYGRVVAAKAYANWQ
                     EGQHLRDPNDLYSAGIEPIYVPTVILGFTDSPDGAPRRKNSVDVKLAVDCVDGSLSNP
                     NIATFVLVTGDGDFVHLINTLRLRGKKVVVIGTSWSTSWQLTSSADHFLAYDVTVDPS
                     SPSVAVKNGTSLSLDDAMKALVDVVKYVREKGRPNVFAQVKLLLTSRLGNFDEQNYGF
                     AKFKDFMKEAEKRGLIKTTTVGLIDRAYMPWENLDEPVAVEAADGAEGRIPIAAPTAD
                     DTTPYVAGQVRGNEESMKTLARFVTKLEESSPFMSFNYIVGRAYESHSLPLSTSEISS
                     LLDEAIQSGVFLTDSRTILDRATGEYRDINIFRLNRQHPVVAAALNQPAKQKVAARDE
                     "
     gene            1172..1807
                     /locus_tag="VHS06_07630"
     CDS             1172..1807
                     /locus_tag="VHS06_07630"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX2988023.1"
                     /translation="MSDAGARDRVPGDRSVTSLILASGSPRREALMRQVGLQFEVEPS
                     DAEESLPEGMTAEESARQLALKKARQVAARRIEGLVIGADTVVVVDGQILGKPSGPDE
                     ARDMIRRLSGREHQVITGIAVVDAATADSRSDSVSTDVRFAPLSQEVIDRYVATGEPL
                     DKAGGYGIQGFAALLVESIRGDYNNVVGLPIRRLAELLGEFGYDAFKMGLG"
BASE COUNT          345 a          553 c          617 g          311 t
ORIGIN      
        1 aggatggaga aggagaacag caagtacagt ttgcttaaca aggagggccg cctccccaac
       61 gccatggcga tcaaggaggc tgccgccaag tacggcaggg tcgtggccgc caaggcatat
      121 gccaactggc aggagggcca gcacctcagg gacccaaacg acctctattc ggcggggatc
      181 gagccgatct acgtccccac ggtgatcctc ggcttcaccg actcgccgga cggcgcgccg
      241 agacggaaga acagcgtcga cgttaagctt gcggtcgatt gtgtcgatgg ttccctgtcc
      301 aatccgaata ttgccacctt tgtcctggtc accggcgatg gcgatttcgt ccacctgatc
      361 aacaccctcc gcttgcgcgg caagaaggtc gtggttatcg gcacctcgtg gagcacgagt
      421 tggcagttga ccagcagtgc ggaccacttc ctcgcctacg acgtgaccgt ggatccctcc
      481 agcccctccg tcgccgtcaa gaacggcaca tccctctcgt tggacgatgc gatgaaggcg
      541 ctggtggacg tggtgaagta cgttcgcgag aaggggcggc ccaacgtctt cgcccaggtg
      601 aaactgctgc tcacctcgcg cctcggcaac ttcgacgagc agaactacgg cttcgccaaa
      661 ttcaaggact tcatgaagga ggcggagaag cgggggctga tcaagaccac taccgtgggg
      721 ttgatcgacc gcgcctacat gccctgggag aacctggatg agccggtggc ggtcgaggct
      781 gccgacggtg ccgaggggcg gatcccgatt gccgccccaa ctgccgacga caccaccccc
      841 tacgtcgcag gccaggtgcg ggggaacgag gagtcgatga agacgcttgc ccgcttcgtg
      901 acgaagctgg aggaaagcag ccccttcatg tccttcaact acatcgtcgg cagggcctac
      961 gagagtcact cccttcccct ctccacctct gagatctcct ctctcctgga cgaggcaatc
     1021 cagagtgggg tgttcctgac cgactcccgg accatcttgg atcgggcgac cggcgagtat
     1081 cgggacatca acatcttccg gctgaaccgg cagcacccgg ttgtggcggc ggcactgaac
     1141 cagcctgcga agcagaaggt ggccgctcgg gatgagtgac gcgggggcca gggatcgggt
     1201 gccaggggac aggtctgtga cctcgttgat ccttgcctcg gggtcgcccc gaagggaggc
     1261 gctgatgcgg caggtgggac tccagttcga ggtggagccc agcgacgcag aggagtcgct
     1321 gcccgagggt atgacggcgg aggagtctgc ccggcagctg gcgttgaaga aggctcggca
     1381 ggtggccgcg cggcgcatcg agggactggt gatcggcgcc gacaccgtcg tggtggtgga
     1441 tggccagatc ctgggcaagc cttccggacc cgacgaggcc cgcgacatga tccgtcggct
     1501 cagcggacgg gagcaccagg tgatcaccgg gatcgccgtg gtggacgccg ctactgccga
     1561 ttctcgctcc gacagtgtgt ccaccgacgt ccgtttcgcc cccctctccc aggaggtaat
     1621 cgaccgctac gtggccacag gagagccgct ggacaaggct ggcggctacg ggatccaggg
     1681 cttcgcggca cttctggtag agtcgatccg cggggattac aacaacgtgg tggggctccc
     1741 catccggaga ctggccgagt tgctgggaga gttcgggtac gacgcgttca agatgggatt
     1801 ggggtgagta gtggggtcgc gcggca
//
LOCUS       DATALS010000341         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_61852,
            whole genome shotgun sequence.
ACCESSION   DATALS010000341 DATALS010000000
VERSION     DATALS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61852"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="VHS06_07635"
     CDS             <1..107
                     /locus_tag="VHS06_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX2988024.1"
                     /translation="AVALAKEYITDAIRYAYPLGKGHGPVNHLYALHR"
     gene            180..1043
                     /locus_tag="VHS06_07640"
                     /pseudo
     CDS             180..1043
                     /locus_tag="VHS06_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
     gene            1114..1410
                     /locus_tag="VHS06_07645"
     CDS             1114..1410
                     /locus_tag="VHS06_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX2988025.1"
                     /translation="MGVDGGVISKYGVVSEEVARAMATVARSTAHSDVGLSVVCGMED
                     GAEGGQRLGLVFCAVDDRGDVTASTTRYTTTWREVQRRSILDAIDLLRRRLLAK"
     gene            complement(1384..>1670)
                     /locus_tag="VHS06_07650"
     CDS             complement(1384..>1670)
                     /locus_tag="VHS06_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988026.1"
                     /translation="GPVEPLMVVPDRRKPTPEEPERGQHPVPHLRMLMDCRYSAQLAL
                     EPSNIIPHSLAAQTSMRMPIRLVGRIDPATRQASHAKHTAARLLRQKPPP"
BASE COUNT          308 a          423 c          603 g          336 t
ORIGIN      
        1 atgcggtggc cctggcaaag gagtacatca ccgatgccat ccggtatgcc taccccctgg
       61 gcaagggaca tggccctgtg aaccacctgt acgcccttca caggtagatc ttcggcggtg
      121 tgtttgatgc ttgggcgtgc aaggcgatat ctgcagtttg tcggagcgtg agagtatata
      181 tgaaagctga gatcctgtct gttgggactg agctgttgct cggacaattg gtcgacacca
      241 atgcccccta cctctcgcaa tcgttgtcaa ccctgggtat cgatgtgttt tggatcagcc
      301 aggtaggcga caaccaggcc agaatggtgg atgtgctgcg tcggggctgg gagcgctccg
      361 acctgatcgt gattagtggg ggcgtcggcc ctaccggcga cgacttgacc cgagagtcga
      421 tagccgagtt gctgggtgag gagatggtcg tggagcccga gctggagaag gagcttcgcg
      481 ccttcttcgc gcggcgcggc aggacgatgc cggaacggaa cgtcaagcag gccactctga
      541 tcccgtccgc ggaagcgctt cccaatccca ttgggaccgc gcctggatgg tgggtcgaga
      601 aggaagggca cgctatcgtt gccatgcccg gcgttcccca cgagatgaag aagatgtggc
      661 acgagcaggt gctgcctcgg ctggccagga tggccgggac tggggtgatc ttgtcgagga
      721 ctctcaaggt ccttggcatg ggggagagct gggtagagga caagttgaag gagttgacct
      781 actccgagaa ccccacgctg gctacctatg ccaaagagga tgggatacac gttcggatca
      841 gcgcgaaggc tcagaacgca gaggctgccg aggcgatggt cgcagatttc gagtcgaagg
      901 ccagggcgac cctgggtgac ctgatctacg gcgtggacga tcagagcgtg cccgaggctg
      961 tgatgtcgct gctggatcga ggcggattga ctctgggaac gatggaagca tcgacggcag
     1021 ggcagctgtc gtcatcgctg gccggggggg gatccggagg gtcggcgctt ccagggggga
     1081 atgattgcct acagccggag tctcctggag aggatgggtg tcgacggcgg ggtgatctcc
     1141 aagtatggcg tcgtgagcga ggaggtcgcc agagcgatgg ctacggtagc tcgcagtacg
     1201 gcgcactccg atgtcgggct ttctgtggtg tgtgggatgg aggacggagc ggagggcggt
     1261 cagcggctgg ggctggtgtt ctgcgcggtg gatgatcgcg gcgatgtgac tgcttctacc
     1321 acccgataca cgaccacgtg gcgggaagtc cagcgacgct caatcctgga tgcaatcgac
     1381 cttctacggc ggcggcttct ggcgaagtaa tctcgcggct gtgtgtttag catggcttgc
     1441 ctgcctggtg gccggatcta tccggcctac caggcgaatg ggcattcgca tagaggtttg
     1501 tgccgccagg ctgtgtggta taatattcga tggttctaga gccagttggg cagagtagcg
     1561 gcaatccatc agcatccgga gatggggtac aggatgctgt ccgcgctccg gttcctcggg
     1621 agtgggcttg cggcggtccg gtaccaccat gagaggttcg acgggtccgg
//
LOCUS       DATALS010000342         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_36537,
            whole genome shotgun sequence.
ACCESSION   DATALS010000342 DATALS010000000
VERSION     DATALS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36537"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            627..1058
                     /gene="mraZ"
                     /locus_tag="VHS06_07655"
     CDS             627..1058
                     /gene="mraZ"
                     /locus_tag="VHS06_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX2988027.1"
                     /translation="MFLGEFQHSIDDKGRLAIPAKFRAPLADGLVLTRGLDRCLYVWT
                     LDQWRELSERLGKLPIMQADARRVARHFFSGASDTSLDKMGRIVLPQFLREYAELGDE
                     VVVAGLYSRIEIWSQENWRAERSLAEEQSSVFAEHLSGLGI"
     gene            1162..>1618
                     /gene="rsmH"
                     /locus_tag="VHS06_07660"
     CDS             1162..>1618
                     /gene="rsmH"
                     /locus_tag="VHS06_07660"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX2988028.1"
                     /translation="MEVRHIPVLYRETIDALRPMEGGRYIDCTLGAGGHAEAILEASG
                     SQSRLLGMDADPEALAVARERLAGYGDRVTLVNSNFRHLAVVAAEAGFVPADGVLMDL
                     GMSSMQLDRWTRGFSFQREAPLDMRFDPRQQVTAADIVNGASEAELKKML"
BASE COUNT          307 a          502 c          496 g          313 t
ORIGIN      
        1 gatcagcctc tcctcctccg agatacagcg cgcacgcacg agcaccgagc cgattacctg
       61 ccagagttgc ggccggatcc tctacctgca cgaatagtcc tgtaccaaca gacgtctcgt
      121 agggcacggg gcgcgagcga gcacagcgcc ccgcttgtgc cccgcctccc acgccgttgt
      181 cacgaccacc gtggtgattc cgaccacgct tcaggcgggg cacaagaccc cgccctacgt
      241 cccatcgaca ccgactccat cagatcagca ttgtctccct caacacgtgc gttaccctgt
      301 tcgaccgaat cctcacattc gtaaccactt tgtaacagta acgggttctg cagctattcc
      361 actgctcaaa cgggcatgcc tcatcagata accccccttc gagaagcggc aaacagggta
      421 cgctacctgc ttttcgaccc cccaaatgtc ggctgcgcgt ttcgccaaga aagggattct
      481 tctactaacg atcttgaaag atgcccagaa tcccttgaaa tgatggggga tcttgcctac
      541 aatgtggccc actgtggata aaagtgggga caagtgggga agcagtgtgg ataactgccc
      601 gatcccccga tcctccggag cttgtcttgt tcctcggcga attccagcac tcgatagacg
      661 acaaagggag actcgcgatt ccggccaagt tccgcgctcc gctggcggac gggctggtgc
      721 tgacgcgtgg gctggacagg tgcctgtatg tttggacact ggaccagtgg cgggaactgt
      781 cggagcgact gggcaagctt cccatcatgc aggccgacgc ccgtcgagtg gccagacatt
      841 tcttctctgg tgcctcggac acgtcgctgg acaagatggg ccggatcgtg ctcccgcagt
      901 tcctgcgaga gtatgccgag ctgggggacg aagtagtggt ggcgggcctc tacagccgga
      961 tcgagatctg gagccaggag aattggcgcg cggagcgctc cctggccgag gaacagagtt
     1021 cggtcttcgc agagcacctc tccggccttg gcatctaggt agctcggggg cagcatccat
     1081 ggtcccgagc gatgctcgga gcgcggtacc acaggcggaa gatcccgccg gagccctttc
     1141 ccccaggata ccgaggtgac catggaagtc cggcacatac cggtcctgta tcgcgaaacc
     1201 atcgatgccc tgcgacccat ggagggtggc cgatacatcg actgcactct tggggcaggt
     1261 gggcatgccg aggcaatcct tgaggcctcc ggctctcagt cccggttgct cgggatggat
     1321 gccgacccgg aggccctggc tgtcgcccgc gagcggctgg ccggatatgg cgaccgtgtg
     1381 acgctggtga actctaactt ccgccacctc gcggttgtgg cggcagaggc cggcttcgta
     1441 ccggcggacg gcgtgctgat ggacttgggg atgtcctcca tgcagttgga ccgatggact
     1501 cgcggcttct cctttcagcg ggaggcgccg cttgatatgc ggttcgaccc caggcagcag
     1561 gtcactgccg ccgatattgt caacggcgcc tcggaggcgg agttgaagaa gatgttgt
//
LOCUS       DATALS010000343         3918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_393457, whole genome shotgun sequence.
ACCESSION   DATALS010000343 DATALS010000000
VERSION     DATALS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393457"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VHS06_07665"
     CDS             complement(<1..664)
                     /locus_tag="VHS06_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX2988029.1"
                     /translation="MVVAAQRFVTPRAPTAVPPSPELPMTTAISTSTIICRLLREAEA
                     HPNKPCLVCPEAGTTLTFSGLVRAIGQLTHTLNTHGIGERDRVALLLPNSPEFVISYF
                     AGIVAGGVVVPIDPRLSRPEVEGLLRHSGARMLIVDSDVDNIPHALGGITLASTSYRL
                     TCLQLEGGRRGVDDPELRPGDCLIVYTSGVSGPPKAVLLHHGNLVASVENIVRSYALT
                     PED"
     gene            complement(889..1932)
                     /locus_tag="VHS06_07670"
     CDS             complement(889..1932)
                     /locus_tag="VHS06_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX2988030.1"
                     /translation="MMRQIRALCALLAIGTALLATAAGAAEPGDVVLPVPQRTQADGT
                     VWASSNCGPAAISEVLEAFGQKLPTKVLRDRANQLLGIADPNTGTRVQDLAQVVREHG
                     LAVTGPYDGKRFRRWTLDDVRAEIQAGHPVVAQVYYPLLPNHSKSGISTDHYIVLMGT
                     SGDGFIFNDSADRDAPGYRQPITAAQLTKAWGSSHQPFGGFSVAPGEGSPSLLPPMPE
                     PSPTPDAQDQGAPADSSPASANAPIEATGTVGTAALAPEVPTMPPTQTQAVASATPVP
                     TETPTPTVPVASATPTPTKIAPAPAPTATATPSAQQVAQSIQATASPSKAAGQQAAQG
                     TLISQLKSGLANQ"
     gene            complement(2221..3456)
                     /gene="tyrS"
                     /locus_tag="VHS06_07675"
     CDS             complement(2221..3456)
                     /gene="tyrS"
                     /locus_tag="VHS06_07675"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX2988031.1"
                     /translation="MVANVLDVLMERGFVKDISDERGLRSALENPITMYIGFDPTASS
                     LHVGNLLSIMVLAWMQRLGHRPIALVGGGTGLVGDPTGRTTGRPMLTYEQIEDNLKGQ
                     KAQLARYIDFSEGRALMVNNAEWLRKLNYIEFLRDIGVHFSVNHMLATETYKTKLASE
                     SGLSFLEFNYALLQAYDFLHLYQEYDCILQMGGSDQWANSLAGTDLIRKVEGGQAYVM
                     VTPLITTASGAKMGKSQSGSVWLDPELTSPYQFYQYWINTEDPDVERFLALYTFLPME
                     EVRRLGELRGAELRKAKEVLAFEATRLSHGEEAAREALEASRSLFGGDAGSMKGVPTT
                     TMNASELAQGIPASQLFAQTGLAVSRGEARRLIQQGGAYVNGDRVENVEQMITPHDLV
                     DGSLFLRAGKKKHHRIVVE"
     gene            3621..>3918
                     /locus_tag="VHS06_07680"
     CDS             3621..>3918
                     /locus_tag="VHS06_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX2988032.1"
                     /translation="MAEVAALTVVDVQNDFLPGGALGVLEGDEIVPVLNGYLGRVHEA
                     GLTIYATRDWHPEVTHHFLQYGGLWPPHCIQGTNGAAFSQGLRLPPETIVVSKGM"
BASE COUNT          666 a         1143 c         1304 g          805 t
ORIGIN      
        1 catcctcggg agtgagcgca taggatctga cgatgttctc cacagaggca acgaggtttc
       61 catgatggag aagcactgcc tttggtggtc cgctgacccc ggaggtgtag acaatcaagc
      121 agtcgcctgg ccgcaactcc gggtcatcga cgccgcgccg tcctccctcc agttggaggc
      181 aggtgagccg gtagctggtg ctggccaggg tgatccctcc gagcgcgtgg ggaatattgt
      241 cgacgtcgct gtcgactatc agcatgcgtg ctccactatg gcgcaacagc ccttccactt
      301 cgggtcggct caggcggggg tcgatgggta cgactacacc tcccgccacg attcccgcga
      361 agtaggagat cacgaattcg ggggaattgg gtagaaggag ggccacgcgg tccctctctc
      421 cgattccgtg agtgttcaag gtgtgggtga gctggccgat cgctctgacc aaccctgaga
      481 aggtgagggt ggtgccggct tcagggcaca ccaggcacgg tttgttgggg tgagcctctg
      541 cctctcgcag cagcctacag atgatggtcg aggttgagat ggccgttgtc atcggcagtt
      601 caggtgatgg ggggacggca gttggtgctc gcggggtgac gaatcgctga gccgccacaa
      661 ccatatggtc cccctgacgg cctgctttca gcggcccctg agcggaggag agtcttctac
      721 atgggcgtaa tctagtgatg gtccattaac tctgcattaa cgggatggcc gaacttctat
      781 caatcgtgta atcgctgatg acgatctaca gaagatggcc gcctgccctg gcgaagtcga
      841 tgctccaggg caggcggcca tctcgatgag aagtccgtgt ttgtggatct actgattggc
      901 caggccggac ttcagttgac tgatcagggt accttgagca gcctgttgcc ctgctgcctt
      961 cgatggcgaa gcagtagcct gaatggactg cgcgacctgc tgtgcagatg gagttgccgt
     1021 ggctgtgggc gcgggagccg gagcgatctt cgttggcgtc ggcgttgccg aggctacggg
     1081 cacagttgga gttggggtct cggtcggaac gggtgtcgcc gaggcaacgg cttgggtttg
     1141 cgttgggggc atggtaggga cctccggcgc gagtgctgcc gtgccgaccg tgccggtagc
     1201 ctctatcggt gcgtttgccg aagccggtga ggagtccgcc ggcgcgccct gatcctgggc
     1261 atcgggggtc gggctgggtt ccggcatcgg cggcagcagc gaggggctgc cctcgcccgg
     1321 ggcgacggag aagccgccga agggctggtg gctcgatccc cacgcctttg taagttgggc
     1381 cgcggtgatc ggctgtcggt agccgggggc atccctgtcg gctgagtcgt tgaagatgaa
     1441 gccgtctccc gacgtaccca ttagcacgat gtaatggtcg gtggagatgc cgctcttgct
     1501 gtggttcggc agcagcggat agtagacctg ggccaccacg gggtggccgg cctggatctc
     1561 ggcgcggacg tcgtccaggg tccagcggcg gaaccgctta ccatcgtatg gcccggttac
     1621 cgcgagcccg tgctctcgaa ccacctgtgc cagatcttgg acccgggtgc cggtgttcgg
     1681 gtcggcgatc cccagcagtt ggttggctcg gtccctcagg accttggtgg ggagtttctg
     1741 gccgaacgcc tcgagcacct cggagatggc cgcagggccg cagttggacg acgcccacac
     1801 ggtgccgtct gcctgggttc gttgggggac ggggagtact acgtcacccg gttcggccgc
     1861 cccggccgcg gtggcaagga gggcggtgcc tattgctagg agcgcacaaa gcgcacgaat
     1921 ctgccgcatc atgacggatt ggtcctttcg ccggttatcg gtttgcagcg ccgacagtct
     1981 tcgaaatggg gatggggaga tagtgcgagt cggagcgtgc cggaacacag tacggcacaa
     2041 atgataaccg ggaactaatc acatgtaaaa tgactgctaa tacctggctg ccgagcgtgc
     2101 gcttggagcg gcaatcgcgc cgatggagca ggcttggggg aggactgcag tagcacttcg
     2161 atgtgccggg ccatcagccc cgtgaacatg ccctcaaggc atgttcacgg ggctgagaaa
     2221 ttactcgacg actatcctgt ggtgcttctt tttgccggca cgtaggaaga gggaaccgtc
     2281 cactagatcg tggggagtga tcatttgctc cacgttctcc acccgatccc cgttcacgta
     2341 ggctccaccc tgctggatga gccttcgagc ctcgcctcga gagacggcca gaccggtctg
     2401 ggcgaagagt tgagacgccg gtatcccttg cgccagctcg cttgcattca tggtggtggt
     2461 gggtaccccc ttcatggagc cggcgtctcc gccgaacagc gacctggacg cttccaatgc
     2521 ctccctggcg gcctcctcgc cgtggctgag ccgcgttgcc tcgaaggcga gcacctcctt
     2581 ggcctttcgc agctccgcgc cccgcagttc tcccaacctt cgcacctcct ccatggggag
     2641 gaaggtgtag agggccagga agcgctcgac gtccgggtcc tccgtgttga tccagtactg
     2701 gtagaactgg tacggggagg tcagctctgg atcgagccag acggagccgg attgcgattt
     2761 ccccatcttg gccccagacg cggtagtgat cagcggcgtc accatcacgt aggcctggcc
     2821 gccctccacc ttccggatca ggtcggtccc ggcgaggctg ttcgcccact ggtcgctgcc
     2881 ccccatctgc aggatgcagt cgtattcctg gtagaggtgc aggaagtcgt atgcctggag
     2941 caacgcgtag ttgaactcca ggaaactgag ccccgattcg gaggcaagct tggtcttgta
     3001 agtctccgtg gccagcatat ggttcacgct gaagtggacg ccgatgtctc gcaggaactc
     3061 tatgtagttc aacttcctga gccactcggc gttgtttacc atcagggcgc ggccctcaga
     3121 aaagtcgatg tagcgggcca gctgcgcctt ctgccccttc agattgtctt cgatctgctc
     3181 gtaggtgagc atcggccggc cggtagttcg accggtgggg tcacccacca gacccgttcc
     3241 gccccccacc agcgctatgg gacggtggcc gaggcgctgc atccaggcca acaccatgat
     3301 ggacagcagg tttccgacgt gaagactgga ggcggtggga tcgaagccga tgtacatggt
     3361 tatgggattt tccagcgccg agcgcagtcc ccgttcgtcc gagatgtcct tgacgaagcc
     3421 gcgctccatc agtacgtcta atacgttagc cacgatgcga actcccagtg gttgctttgt
     3481 aggaggatta tagcccattc ggtcgttcct agccttcaac ttcccctgtt ggcacgcctt
     3541 ccgcagtctc gtcgtctcga ggatgtttgc gcctgcaacg gccttcatgg gggccgtacc
     3601 gggaaagggg tgctggacca atggctgagg ttgctgctct aaccgtagtt gacgtgcaga
     3661 acgatttcct gccggggggc gcgttgggag tcctggaggg ggacgagatc gtgccggtgc
     3721 tgaacggcta tcttgggcgg gtccacgagg cgggactgac catatacgcc actcgcgact
     3781 ggcatcccga ggtcacccac cacttcctgc agtatggcgg gctgtggccg ccccactgca
     3841 ttcagggcac caatggggcc gccttctccc agggtctgag gctgcccccc gaaaccatcg
     3901 tggtctccaa gggcatgg
//
LOCUS       DATALS010000344         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_170780, whole genome shotgun sequence.
ACCESSION   DATALS010000344 DATALS010000000
VERSION     DATALS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170780"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VHS06_07685"
     CDS             <1..1225
                     /locus_tag="VHS06_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX2988033.1"
                     /translation="IEQSLSTFSSHVTHIVEILREADPHALVLLDELGAGTDPQEGSA
                     LARAILSDLLERRVPTIATTHYSELKSFAHTTPRVQNASVEFDIETLSPTYRLSIGLP
                     GRSNALAIATRLGMGQEIIDKARALLEPADVEIEGLLSQLQVDRDEARTARAEAERMR
                     DQVRANRARLNAELADLEERKQLLLERARAQSEQELAALRQRVQQTMRELERSKVQAD
                     AASRLESVAQKAESMRPLRAPKPQRRRQPSRQSIEVAELKVGQPVFLASLQTVGVLSS
                     LPDDRGEVEVQVGNLRTRVKMGELSRTDTPKPERRVEPDVTYRFSQQAPDIGIQLDLR
                     GKRPQEAEEELDRYLNDAYLAGLKVVRVIHGKGTGALRQAVREQLAGSPLVRHFEPAD
                     ARDGGEGATVVNMAN"
     gene            1381..1839
                     /locus_tag="VHS06_07690"
     CDS             1381..1839
                     /locus_tag="VHS06_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2988034.1"
                     /translation="MDKLIQTALIVVGAYLLALWVAAIWWTYRDIRNRTRDILLQLAA
                     TLLVAVFSFPGLLIYQVLRPPKTLSDVYATALEEEALLQDIEIRTACPQCKHRTEEEF
                     IFCPNCATRLKRVCSNCRRPLSLRWNTCPYCGEGVDALSGMRERETARRG"
     gene            complement(1920..>2665)
                     /locus_tag="VHS06_07695"
     CDS             complement(1920..>2665)
                     /locus_tag="VHS06_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988035.1"
                     /translation="STSRSDSWIKPFILIDGKPVDRELNEVLMEFDGGDVYIKGANAV
                     DAEGNAGVLVASKVGGNIGTAMGMVLARGAHVIIPVGLEKLVPSVIDASRKCGIKKLK
                     YPAGATLGLIPVVSGTVITEIEALRILTGVHATHVASGGIGGSEGTVVLVVEGPDDRV
                     RAAFKIWEQIKGEPPRPCVSHHRSLRHGRQIAGVTLPSATLPQGDACVAPTGNKTAPV
                     DRSTGAVGSSLGVGGVPDPDGLSRHRPYK"
BASE COUNT          534 a          834 c          844 g          453 t
ORIGIN      
        1 catagaacag agtctgtcca ccttctcctc ccacgtgact cacatagtgg agatcctgag
       61 ggaggcggat ccccatgccc tggtgctgct ggacgagttg ggcgcgggga cggatccaca
      121 ggagggttcc gccctggcca gggcgatcct ttccgacctg ctggaacggc gggtgccgac
      181 catagccacc acacactact ccgagctgaa gagcttcgcc catacgacgc cgcgggtgca
      241 gaacgcctca gtggagttcg acatcgaaac gctatcgccg acctacaggc tcagtatcgg
      301 gctgcccggc cgcagcaacg cgctggccat tgcgactcga ctcgggatgg ggcaggagat
      361 catagataag gcaagagcgc tcctggaacc cgccgacgta gagatcgagg ggctgctttc
      421 ccagctacaa gtcgaccggg atgaagcccg caccgccagg gcggaagccg aacggatgag
      481 ggaccaggta cgcgccaatc gagcaagact gaacgccgag ctggccgacc tggaggagcg
      541 aaagcaactg ctactcgagc gggcaagggc tcagtcggag caggagctgg ctgcactcag
      601 gcaaagggtg cagcagacga tgcgggaact ggagcgcagc aaggtccagg ccgacgccgc
      661 atccaggctg gagtcggtcg cccaaaaggc cgagtcgatg cgccccttga gagctccgaa
      721 gccgcagcga cgccgacaac cctcccggca atcaatcgag gtcgcggagt tgaaggtagg
      781 ccaaccggtc ttcctggcca gtctccagac ggtgggcgtc ctctcctcac ttcccgacga
      841 tcgaggcgag gtagaggtcc aggtgggcaa cctgcgaacc cgggtgaaga tgggggagct
      901 gtctcgaacc gacaccccga aaccggagcg acgagtagag ccggacgtga cttaccgatt
      961 cagccagcag gcgccggaca tcggcattca gctggatctg cgcggtaagc gaccgcagga
     1021 ggcagaagag gaactggaca ggtatctgaa cgacgcctac cttgctggac tgaaagtggt
     1081 gcgtgtgatc cacggcaagg gcactggggc gctgagacaa gccgtccgcg agcaacttgc
     1141 cgggagccct ctggttcgcc acttcgagcc cgcggacgcg cgggacggtg gcgagggcgc
     1201 taccgtggtc aacatggcca actagaatcc agctcatcct gtgtatcctg tttggtcccc
     1261 gtgccacagg cctcgcacac ctcggctaca agtaacaagc tgtgttacaa tggagtccgt
     1321 tcttttttgg cacccttcgt gcttttcccc atgcagcaac tctgaaagag gttcagccga
     1381 atggacaagc tgatacagac ggctttgatt gtagtggggg cctacctgct cgcactatgg
     1441 gtggccgcta tctggtggac ctaccgagac attcgcaaca gaacccgtga cattctgctc
     1501 caactggctg ccacgctcct cgtggccgtc ttcagcttcc cgggtctgct gatctaccag
     1561 gtgttgaggc ctcccaagac gctgtccgac gtctatgcca ccgctctgga agaggaggcc
     1621 ctgctccagg acatcgagat ccgcaccgcc tgcccccagt gcaagcacag aacggaggag
     1681 gagttcatct tctgccccaa ctgcgcaacg aggctcaaga gggtgtgctc caactgtcga
     1741 cgccccctgt cgttacggtg gaacacctgc ccctactgcg gtgaaggcgt ggatgcgcta
     1801 tccggcatga gggagaggga aaccgccagg aggggatagc gattcggtct tgacgctagt
     1861 atcttgacag gatcaccctg tcactaggag cccgggtcgt ccctgacccg ggcggtggct
     1921 catttgtacg gacggtggcg cgagaggccg tcggggtcag ggacgccccc gactcctaga
     1981 gaagaaccaa cagcccctgt ggatcgatcc acaggggctg ttttgttacc tgtaggggcg
     2041 acgcatgcgt cgccctgcgg aagcgtggca gatggcaggg tcacaccggc gatttgacgt
     2101 ccatgccgaa gcgagcggtg atgggagaca cagggacggg gcggctcgcc cttgatctgc
     2161 tcccagatct tgaaggccgc gcgcacacgg tcatccgggc cctcgaccac cagcaccacg
     2221 gtgccctcgg agccgcctat gcctccggac gccacgtggg tcgcgtggac gccggtgagg
     2281 atcctcaacg cctcgatctc agtgataacc gtgcccgaca cgacagggat cagcccgagc
     2341 gtcgcgcctg ccggatactt cagcttcttg atgccgcact tccgcgatgc gtcgatcacg
     2401 gaagggacca gcttctccag ccccacggga atgatcacat gcgccccgcg ggccagtacc
     2461 atgcccatgg cggtgccgat attcccaccg accttgctgg cgacgagcac accggcattg
     2521 ccctcggcgt cgacggcgtt ggcgcccttg atgtagacgt cacctccgtc gaactccatc
     2581 agcacttcgt tgagctcacg gtccaccggc ttcccgtcga tcaggatgaa gggtttgatc
     2641 caggagtcgc tccgggaggt ggagc
//
LOCUS       DATALS010000345         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_170810, whole genome shotgun sequence.
ACCESSION   DATALS010000345 DATALS010000000
VERSION     DATALS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170810"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(896..2034)
                     /locus_tag="VHS06_07700"
     CDS             complement(join(896..1797,1797..2034))
                     /locus_tag="VHS06_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611297.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX2988036.1"
                     /translation="MARRKFTREFKISAVKLVNEQGYTISEAAKSLGVDDNSVRYWIK
                     QLASEPGLAPTGDGALAAEIQRLRKENARLLLERENLKKSGRVLCQGASVKFRFIDQH
                     LQQFPVEAVCEVLRVSRSGYYAWRGRPRSNQEARREELARKIQAVHQQSRRVYGSPRV
                     CKALQAEGQRVCENTVARVMRQQGIRAKTKKTFVPRTTESLHEQPVAENLLNREFTAD
                     RPNQKWVTDITYIPTDEGWLYLAGVMDLFSRKIVGWSMAEHLRTDLVSEALRMAIARR
                     QPGAGLLHHSDRGIQYASDAYQQLLQAHRMEASMSRRGDCWDNACAESFWGTLKTELV
                     NHEHYASRDAARQSIFEYIEVFYNRNRLHSSLGYQSPEAFEASLN"
     gene            2237..2365
                     /locus_tag="VHS06_07705"
     CDS             2237..2365
                     /locus_tag="VHS06_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988037.1"
                     /translation="MTVGSNLEGQLEMAVRRIEPDGFSEDRRTTVQDGDEDEGQVF"
BASE COUNT          464 a          738 c          852 g          503 t
ORIGIN      
        1 acctgagcat cttgccggcc catggcaatc cacctcgcat atcacctcgc gccactggat
       61 atcctgcagg ctaaagggcc tgtcagccat gtcctctcct tccttgtcca caccggcgat
      121 gaagactcat atccgaaccg ccgggagatc ggcgattata gaagagggtg gttggggcgg
      181 gaaggcggag tgggggaaag gccaagggac gggagggacg gagggaacat gcgcaggatg
      241 cgcggcggtg gctggcactg ggttcctggt gcctgaggca gggcaacatg cgcaggtttc
      301 ggggggtgtc gcgcgcgtct ggcagggttg ctgggtgagg tggatcaccg tggcggcgtt
      361 cggtacttgg ttcctggtac ctgagacaga ccaaggtgct cagattgggg aggaaggcag
      421 gaaggggcaa agggcaagac gcgcaggttt ccaatggggt cgcgcgcgtg gtcgcgggca
      481 ttgcctactt gtccggtagg agtacgggta ttcggttgcc aaagagcggg cagtgctccg
      541 gtgccaaatg gcgaaagcag aaaccagaaa gcagaaagaa acactcgccc ggaggggcgg
      601 aaaagcgcgc cctccgtata taaggggtgg ttaacgatgg ggcagagttg ggggatgagt
      661 gtttgcaagg ggttaggagc ggggaggatt ctttcgtgat tttggggagc tgatctcacg
      721 cgaagaagca aggacgcgaa gggccatgct gttctgaaca ggagggaaca gaggaagagg
      781 ggttcgcgca gaggcgtagg gacgcggaga cgcagggacg ggcccaggca ctctgggttc
      841 gggactcagg gttggagatt gagcttcccc acgaatggtg ggcgcgccga ggtaactaat
      901 tcaggctggc ctcgaacgct tcggggcttt ggtagcctaa cgaactgtgt agacggtttc
      961 tattgtagaa cacctcgatg tactcgaaga tcgattgcct ggccgcgtcc cggctggcgt
     1021 agtgctcgtg gttgaccagc tcggtcttga gcgtgcccca gaagctctcg gcgcaggcgt
     1081 tgtcccagca gtctcctcgc cggctcatgc ttgcctccat gcggtgcgcc tgtaagagct
     1141 gctggtaagc gtcgctggca tactggatgc cccgatcgct gtggtgcagc agccctgcac
     1201 cgggttgacg acgcgcaatc gccatccgca gggcctcgct caccagatcc gttcgcaggt
     1261 gctccgccat cgaccacccc acgatcttcc gcgagaacag gtccatcacc cccgccaggt
     1321 acagccagcc ttcgtccgtc gggatgtagg tgatgtccgt cacccatttc tggttggggc
     1381 gatcggcagt gaactcacgg ttcagcaggt tctcggccac cggctgctcg tgcagactct
     1441 cggtggtgcg cggcacgaac gtcttcttgg tcttggcccg gattccctgc tgacgcatca
     1501 cccgcgccac cgtgttctca cacacgcgct gcccttcggc ctggagggct ttgcacacgc
     1561 gtggactgcc gtagacacgc cggctctgct ggtgcacggc ctgtatcttc cttgccagct
     1621 cttctctgcg tgcctcctgg ttgctgcggg gccggcctcg ccacgcgtag tagccagagc
     1681 ggcttaccct caatacctca cagacggctt ccaccgggaa ctgctgcagg tgctggtcga
     1741 tgaagcggaa cttcacagat gctccttggc aaagaacgcg gccgcttttt ttaagatctc
     1801 ccgctccagc agaagacggg cgttctcctt ccgcagacgc tggatctcgg ccgccaaggc
     1861 gccatcaccc gtgggcgcca gccccggctc cgaagccagc tgtttgatcc agtagcggac
     1921 gctgttgtcg tccactccca ggctcttggc cgcttcagag atggtgtatc cctgctcatt
     1981 gaccagcttc acggccgaga tcttgaactc ccgcgtaaac ttccgtcgtg ccatcttccg
     2041 ctccttccgg cagcattatg ctgccttccg gcgcgcccac cattcgtggg gaagctcaaa
     2101 ccccttttcc cacccccttt tcccaccgcc cacccctttt cccaccgacc ggtagagagc
     2161 gacgacgctt agggtttggt tttcacgagc acgcaggcgc cgccgccggg ccgtccaccg
     2221 ccagagcgtg cgtcaagtga ccgtcggcag caacctcgaa gggcagcttg agatggccgt
     2281 ccggagaatc gagccggatg gtttcagcga agaccgccga accacagtac aggacggtga
     2341 tgaggacgag gggcaggtgt tctagtttca ttagaggaag atgaatgagg aatcattcga
     2401 gcgtgatctt gaagacgagg gcggcgtcgg aggctggctt ggcggaggcg ggagtgacag
     2461 tgagcgcgtc gtcgccgagg gaccaggcga tggtttccgt cggcgcgccg agttgttgga
     2521 tcctggcgac gtggcggtcg aggagagcgg cggtttt
//
LOCUS       DATALS010000346         7072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_287174, whole genome shotgun sequence.
ACCESSION   DATALS010000346 DATALS010000000
VERSION     DATALS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287174"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..131
                     /locus_tag="VHS06_07710"
     CDS             39..131
                     /locus_tag="VHS06_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988038.1"
                     /translation="MESWLQETAQSLGVELQFFQSNWEGATCLS"
     gene            complement(95..1204)
                     /locus_tag="VHS06_07715"
     CDS             complement(95..1204)
                     /locus_tag="VHS06_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2988039.1"
                     /translation="MTEGPNQVFETIWAQAAKVFSAEAFSVALCGPGPNQITYELVVD
                     RGVRQVPQTSDLGDGLSQLVVRSGKPVLVRDFELEDGTMPLSWMGAPMLNGDRVIGLL
                     IIQASRPDAFERRDLSVLSMLANWAAFAVDSSQRFQTQQQEAEAASALLQAARVLGRE
                     TDPDGLFRAVAETASTVVDCVRCSVWLWSADDQHFAPMWRSSVGKRKAEQFTSAPMRP
                     TEIPAVVDMLRSQDVVVVGENESALLGVVGDALPLGLRSIALVPLARDGEIVAQMVIV
                     RSSSGEEFTPKEIDMLRGIADIVGLTLNNLRQHKQAGEAAALREVSEVKSRLISTISH
                     ELRTPLSFVQAGSELLMQRLFEPDQLRQVAPSQLL"
     gene            complement(1247..1696)
                     /gene="aroQ"
                     /locus_tag="VHS06_07720"
     CDS             complement(1247..1696)
                     /gene="aroQ"
                     /locus_tag="VHS06_07720"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX2988040.1"
                     /translation="MKVAVVNGPNIDILGKREPKVYGTTTLAEIESWLQETAQSLGVE
                     LQFFQSNWEGAIIDYLHGLEGTVQGLIINPAAFSHYSVAVRDALAALSIPVVEVHLSN
                     IYAREGFRHHSITSEVVTAQISGLGYRGYLLALEGLLALEETAARTE"
     gene            complement(1769..2854)
                     /gene="aroB"
                     /locus_tag="VHS06_07725"
     CDS             complement(1769..2854)
                     /gene="aroB"
                     /locus_tag="VHS06_07725"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX2988041.1"
                     /translation="MIRIPVRAATGEYDVLVGQGLLEEAGSLFSEAGLKGEVRIVADE
                     QVYGLYAQTLEESLGRAGFRVDSLKVPSGESSKSLQMASRIYDWLIEGRTERRDVVVA
                     LGGGVVGDLAGFVAATFLRGLRFVQVPTSLLAQVDSSVGGKVAVNHPLGKNLIGSFYP
                     PALVVADTSTLASLPPRELSAAFAEIAKMGIILDRSLFERLEAEADLLLRLDRQSLTQ
                     VIARTVELKTQVVQQDERESGLRAILNYGHSYGHAVEVVTDYELYRHGEAVAIGMVGA
                     GMLARRLGMIDDDTVARQNRMLERFGAPIRCPRLPVDRMLEAMTHDKKATRGRLKWVL
                     LEGIGKVTVRQDVPEDLVRHVLEELMD"
     gene            complement(2851..3399)
                     /locus_tag="VHS06_07730"
     CDS             complement(2851..3399)
                     /locus_tag="VHS06_07730"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX2988042.1"
                     /translation="MNVVLIGFSGSGKSTVGKLLAKRLGWEFVDTDERIEHATGKPIH
                     LIFAEEGEASFRKLESEAVNSALQGDSRVIAVGGGAVTDPVSRYRMRDGNLVLMLDAE
                     VSTLHDRLATAVVDEPRPMIADPDPLARIEALKALRDPIYREVAHLIVSTEELDVEGV
                     VERIVSLVFSTPDERPVAEAER"
     gene            complement(3396..4598)
                     /gene="aroC"
                     /locus_tag="VHS06_07735"
     CDS             complement(3396..4598)
                     /gene="aroC"
                     /locus_tag="VHS06_07735"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX2988043.1"
                     /translation="MLSVLRMLTAGESHGQALVGILEGVPSGLPLETGDIDLQLRRRQ
                     GGYGRGGRMKIEKDQVQLLSGVRLGLTLGSPIAMQVVNRDWTQWTERMSVEPRGTAPE
                     ALTRLRPGHADLAGVEKYAHADVRNILERASARETTTRVALCAVARKLLSRFGVTIRS
                     HTIAVGGVAATTPDWVTTDRRGGNSPQAERYWGDVESSPVRCGDPSAVDAMIAAIDEA
                     RKDGDTLGGVFQVVAYGIPPGLGSHVQWDRKLNGHLAEALMSINAIKGVEVGSGFSLA
                     GLRGSAVHDVIGRLAGGEMIRRSNNAGGIEGGMSNGEPIVVNCAAKPIPTLMNPLPSV
                     DLASGLVVPAAQERSDVCVVPAAGVVGEAMVSLVLADAFLEKFGGDSVSEIERNYEGY
                     LQSLGVAR"
     gene            complement(4696..5121)
                     /locus_tag="VHS06_07740"
     CDS             complement(4696..5121)
                     /locus_tag="VHS06_07740"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988044.1"
                     /translation="MIGFLIEHSISPAIHQPALDTLGITARYERLRVAPEGLRSFVEG
                     LRGGGWLGVNVTVPHKEAVVPLLDALDTEAEAIGAVNTIVATDATLVGYNTDARDRGV
                     RTLGGLPMLVYQAAASFELWTGRAAPVEIMMKAAEGALV"
     gene            complement(5133..6452)
                     /gene="aroA"
                     /locus_tag="VHS06_07745"
     CDS             complement(5133..6452)
                     /gene="aroA"
                     /locus_tag="VHS06_07745"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041436416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX2988045.1"
                     /translation="MTGSTQHPVMVRRPARLRGELRVPGDKSISHRALILNAIADGEA
                     GVSNLCPGADVASTARCLRALGVAIEPDGDAGSLRVRGVGLEGLREPEQVLDAGNSGT
                     TIRLLSGLLAGQPFFATITGDDSLRARPMGRIIKPLRLMGCDVWGRRGDTLPPLAIRG
                     GGLKGIDYALPVASAQLKSALLLAALFAQGPTTVREPSPSRDHTERMLLAQGADLRVE
                     GTSISLEPGRRLSPVDVQVPGDISAAAFWLVAAAIHPDAEIVVRGVGVNPGRTGVLDV
                     LHAMGADLEVIPTGEQGGEPVADLVARSSELRGIEIGGSLVPRLIDEIPVLAVASAFA
                     RGESRFVDVGELRVKETDRLRALAVEMARMGASVAESADGLTVSGGGGLHGARCLSYK
                     DHRMAMAMAVAGLAASGQTVIDDPGVAEISYPGFWADLRRVSEGELE"
     gene            complement(6452..>7072)
                     /gene="aroF"
                     /locus_tag="VHS06_07750"
     CDS             complement(6452..>7072)
                     /gene="aroF"
                     /locus_tag="VHS06_07750"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX2988046.1"
                     /translation="MGEEGLKILSEVGQKTGLKVITEVMEPEQVDVVAANADILQIGA
                     RSMQNFPLLREVGRVDKPVLLKRGMAATVDEWLMAAEYIMAEGNFNVILCERGIRTFE
                     TAVRNTLDLSAVPVVKRLSHLPIVVDPSHGTGKWYLVKSMAMASVAAGADGLLIEVHP
                     TPDTASCDGPQSLTIPHFRDMMDMLRVVGSAMGKRLPAASHDLAVA"
BASE COUNT         1339 a         2493 c         1964 g         1276 t
ORIGIN      
        1 gggagcccaa ggtctacggc acgacgacac tcgcggagat tgagagctgg cttcaggaaa
       61 cggcgcagtc tctaggtgtc gaacttcagt tctttcagag caattgggaa ggggccacct
      121 gcctgagttg atccggctcg aacaacctct gcatcaatag ctcgctgccg gcctgaacga
      181 agctgagcgg ggtccgcagc tcgtgggaga tggtggaaat cagccgcgac ttgacctcac
      241 tcacttccct cagtgcggcc gcctcgccgg cctgtttgtg ctgtctgagg ttgttgaggg
      301 tcagtccgac aatatcggca atccccctca gcatgtcgat ctccttgggg gtgaactcct
      361 ccccggaact cgaccgcacg atcaccattt gcgccactat ctcgccgtct ctggccagcg
      421 ggaccagcgc tatggaacgc aaccccagcg gcaacgcatc gccgactact ccaagcaggg
      481 cgctctcatt ctcgccgaca accaccacat cctgactacg cagcatgtca acaactgcgg
      541 ggatctcggt tggcctcata ggggcggaag tgaactgctc cgccttccgc tttcccacgc
      601 tgctgcgcca catcggcgcg aaatgctggt cgtcggcaga ccagagccac accgaacagc
      661 gcacacagtc aacgaccgtg gaggcggttt cggcgactgc cctgaacagc ccatccgggt
      721 cggtttccct gcccagcacc cgggcggctt ggagcaaggc agacgcggcc tcggcctcct
      781 gctgttgagt ctggaaccgt tgggatgaat cgacggcgaa ggcggcccag ttggcaagca
      841 tggagagaac cgagaggtct ctgcgttcga acgcatcggg gcgagaagct tgaatgatga
      901 gaaggccgat taccctatcc ccatttaaca tgggggcccc catccaggat aggggcatgg
      961 tgccgtcctc cagctcgaag tcgcgaacca gcacaggctt gccggaccgc acgaccagct
     1021 ggctcagccc atcgccgagg tcgctggttt gcggaacttg cctgacgccg cgatcgacga
     1081 ctagctcgta tgtgatctga ttgggacccg gcccacagag agcaacggaa aaggcctcgg
     1141 cagagaagac ctttgccgcc tgcgcccaga tcgtctcaaa gacctgattc ggtccttcgg
     1201 tcacccttcg ctcccataac tcagaaccac aacacttaca ctactattat tcggttcggg
     1261 ctgccgtttc ctcaagggct aatagtcctt ctagcgctag cagatagccc cgatatccta
     1321 atcctgagat ctgggccgtc accacctcgg aggtgatgga gtggtgtctg aacccctctc
     1381 gtgcgtagat atttgacagg tgcacctcta ctacggggat ggagagtgcc gcaagcgcat
     1441 cccgaacggc gacactatag tggctaaatg cagcgggatt gatgatcagg ccctgaactg
     1501 tgccctccag tccatgcaga tagtcgatta tggccccttc ccaattgctc tgaaagaact
     1561 gaagttcgac acctagagac tgcgccgttt cctgaagcca gctctcaatc tccgcgagtg
     1621 tcgtcgtgcc gtagaccttg ggctccctct tccccaggat gtctatgttc gggccgttta
     1681 ccaccgcgac cttcaaccgc tcaccctccc cgtcggagaa tcccgacgcg ctaccactta
     1741 gtataatcgg ccatcagttg ccgattcctc agtccatcaa ctcctccagc acatgcctca
     1801 ccagatcctc cggaacatct tgccgaacgg tcaccttgcc aattccctcc agcaacaccc
     1861 acttcagtct tccccgagtg gccttcttgt cgtgagtcat tgcctcaagc atacgatcga
     1921 caggaagccg agggcagcga atgggggctc cgaatcgctc gagcattcga ttctgccgag
     1981 caaccgtgtc gtcgtcgatc atccccagcc tgcgagcgag catgccggcc cccaccatac
     2041 cgatggccac tgcctcgcca tgccggtaca gctcgtagtc ggttaccact tcgaccgcat
     2101 gcccgtagct gtggccgtag ttcagaatcg cccgcagccc ggactcccgc tcgtcctgct
     2161 gcaccacttg cgtcttcaac tccacagtcc tggcgatcac ctgcgtcagg gattgccggt
     2221 ccagcctcag taggagatcg gcctccgcct ccagccgctc gaagagcgac ctgtccagaa
     2281 tgatccccat cttggcgatc tcggcaaacg cggcggaaag ctcgcgcggg ggcagcgacg
     2341 ccagagtgga cgtatccgca accaccagag ccggaggata gaaggaaccg atgaggttct
     2401 tcccaagggg gtggttcact gccaccttcc ccccaacgct ggagtcaacc tgagccagca
     2461 ggctggttgg gacctgaacg aaacgaagcc cccgaaggaa ggtggccgcg acgaaccccg
     2521 ccaggtcgcc cacgacgcca ccgccgagcg ctactacgac atcacgccgc tcggtccggc
     2581 cctcgattag ccaatcgtag atcctggaag ccatctgaag agacttgctg gactcgccgg
     2641 atggtacttt gagcgagtcc actcgaaaac cggctcgccc aagcgactct tccagcgtct
     2701 gcgcgtacaa gccatacacc tgctcgtccg cgacgatgcg gacttcgccc ttgagaccgg
     2761 cctctgagaa gaggctccct gcctcctcca acagcccctg tccgaccagt acgtcatact
     2821 cgccggtagc tgcgcgaacc ggtatccgga tcatcgctcc gcctccgcga cgggccgctc
     2881 gtcgggtgtt gagaagacca gagagacgat cctctccacc acaccctcca cgtcgagttc
     2941 ttcggtggat acgatgaggt gggcaacttc cctgtatatg ggatcgcgaa gcgccttcag
     3001 cgcttcgatg cgagccaggg ggtcggggtc agcaatcatc ggcctcggct catccaccac
     3061 ggccgtcgcc agcctgtcgt gaagcgtaga cacctcggcg tccagcatca gcacgaggtt
     3121 gccgtctctc atccgatacc ggctcaccgg atcggtcacc gctccccctc caaccgcgat
     3181 gacgcggctg tctccctgaa gcgcggagtt cacggcctca ctctccagtt tccgaaagga
     3241 cgcctcgccc tcctcggcga aaatcaggtg gatcggcttc ccagtggcgt gctctatcct
     3301 ctcgtccgta tcaacgaact cccagcccaa ccgtttcgcc agtagctttc caactgtcga
     3361 tttcccagat ccactgaagc cgatcagcac aacattcatc gcgcaacccc cagcgactga
     3421 aggtatcctt cgtagttcct ctcgatctcc gacacggaat cgcctccaaa cttttccagg
     3481 aaggcgtcgg ccagcaccag cgacaccatc gcctcgccca ccacgcctgc ggctggaact
     3541 acgcagacgt cgcttcgctc ctgggcagcc ggcacgacca atcccgacgc caggtccacc
     3601 gaaggaagcg gattcatgag ggttgggatc ggcttcgccg cgcagttcac cacgattggc
     3661 tctccgttgc tcattccgcc ctcgatcccg cccgcgttgt tgctccgtcg gatcatctca
     3721 cctccggcca accgcccgat cacgtcgtgc acggccgatc cgcgaagccc agccagactg
     3781 aacccgcttc ccacctccac gcccttgatg gcgttgatgc tcatcagcgc ctcggcaaga
     3841 tggccgttca gcttacgatc ccactgtacg tggctgccca gtccgggtgg tatcccgtag
     3901 gcaacaacct ggaacacccc accaagcgtg tctccgtcct tccgagcctc gtcgatggcg
     3961 gctatcatcg catctacggc cgaggggtcg ccgcatcgca caggggacga ctccacatcg
     4021 ccccagtagc gctccgcctg cggggagttg ccgcctcgac ggtcggtggt cacccaatca
     4081 ggcgtcgtgg cggccacgcc accgacggct atagtgtgac tgcgaatcgt gacaccaaaa
     4141 cgagagagca gcttcctggc aacggcgcaa agcgccaccc tggtggtcgt ctcgcgagcg
     4201 ctggctcgtt ccaggatgtt ccggacatcg gcatgagcgt acttctccac ccccgcgagg
     4261 tcggcgtgac cgggcctcag ccgtgtcagc gcctccggcg ccgtcccgcg aggctcaacg
     4321 ctcatcctct ccgtccactg cgtccagtcg cgattgacga cctgcatggc gatgggactg
     4381 cccagggtga ggccgagccg cacgccggag agcaactgca cctgatcctt ctcgatcttc
     4441 atcctgccgc cccggccgta cccgccctgt cggcggcgca attgcaggtc gatgtcaccg
     4501 gtctccagcg ggaggccgga gggaacaccc tccagtatgc ccaccagggc ttgcccgtgg
     4561 gattcgcctg cggtaagcat ccggaggacg ctcagcatcc tagtacctcc aagtgttcca
     4621 cgacagtgag ttcagtggcg taggggcgac gcatgcgtcg cccctacggg tcacgatcac
     4681 gaaagggaga ggcgatcaga ccagtgcgcc ctccgccgct ttcatcataa tctccacggg
     4741 cgcagccctg ccggtccaca gttcgaaaga agctgccgcc tggtaaacca gcatcggcag
     4801 gccgcccagc gtcctcaccc cgcggtcccg cgcatcggta ttgtacccca caagcgtcgc
     4861 gtccgtggca acgatggtat tcacggcacc tatggcctcg gcctcagtat ccaatgcgtc
     4921 cagcaagggg accacggctt ccttatgggg caccgtgacg tttacgccaa gccacccgcc
     4981 gccccgcaat ccctccacga aggaccgcaa cccctccggg gccacccgca gtcgctcgta
     5041 tcgggcggta atgccgagag tatccagagc cggctgatgt atcgctggcg atatgctgtg
     5101 ttcgatgaga aagccgatca tcgccggccg cttcactcca gttccccctc ggagactcga
     5161 cgcaggtccg cccagaatcc cggataggat atctccgcca cgccgggatc gtcgataacg
     5221 gtctgtcctg acgccgccag tcccgccacc gccatagcca tcgccatcct gtgatccttg
     5281 tagctgaggc agcgagcgcc atgcagccca ccgccaccgg agaccgtcaa cccgtcggcg
     5341 gactccgcaa ccgacgcacc catcctggcc atctccaccg ccagcgcgcg caatcgatcc
     5401 gtctccttta cccgaagctc cccgacatct acgaatcggc tctcgccccg tgcaaaggcc
     5461 gacgctacgg ccagcactgg gatctcgtct atcaacctgg gcaccaggga cccaccgatc
     5521 tcgatacccc ggagttcgct ggacctcgcc accaggtcgg ccacgggctc cccgccctgt
     5581 tcgcccgtcg ggatcacttc cagatccgcg cccatagcgt gcaacacatc cagcacgccg
     5641 gtgcggcccg gattcacccc cactccgcgt accactatct ccgcgtcggg gtgtatggcg
     5701 gccgccacta accagaaggc cgccgcagag atatcgcctg gcacctgcac gtccactggc
     5761 gaaagccggc gcccgggctc cagagatatg ctcgttccct cgacgcgcag gtcggccccc
     5821 tgggccagca gcattcgctc ggtgtgatcc cgagatgggc tcggctcccg aaccgtagtg
     5881 gggccctgtg caaagagcgc ggccagcagg agggcggact tgagttgtgc gctggccact
     5941 ggtagggcat agtcgatccc tttgagcccg ccgcccctga tcgccagggg gggcaacgtg
     6001 tcgccacggc ggccccacac gtcgcacccc ataagcctca gcggcttgat gatcctgccc
     6061 attggacgag cccggaggga gtcatcgccg gtaatggtgg caaagaaggg ctggccggcc
     6121 agcagtccag acaataggcg gatagtcgtt ccggagttgc ccgcgtccag cacctgctcc
     6181 ggctccctca acccctccag ccccacgccg cgtaccctga gactgcccgc atctccatca
     6241 ggctcgattg ccacacccag cgccctcagg caacgggcgg tggaggcaac atccgccccg
     6301 gggcacaagt tggacacacc ggcttcgccg tccgcgatgg cgttgaggat cagggcacgg
     6361 tgagagatgg acttgtcacc cggaacccga agctcgcccc ggaggcgcgc gggccggcgc
     6421 accataaccg ggtgctgagt gctgcccgtc atcacgccac cgccagatcg tgcgacgccg
     6481 cgggcaggcg cttgcccatg gccgagccca caaccctgag catatccatc atatccctga
     6541 agtgggggat ggtgagcgac tgaggcccat cgcaactggc ggtgtccggc gtgggatgca
     6601 cctcgatcaa cagtccgtcc gccccagctg ccacagaggc catggccatc gacttgacga
     6661 ggtaccactt gccggtaccg tggctggggt ccaccacgat cgggagatgg ctcaaccgct
     6721 tcactacggg aacggcgctg aggtcaaggg tattgcgaac ggcagtctcg aaggtacgta
     6781 tcccccgctc acacagaatc acgttgaaat tgccctcggc catgatgtac tcagccgcca
     6841 tcagccactc gtcgacggtt gccgccatcc cccgcttcaa cagcaccggc ttgtccaccc
     6901 tgccgacctc cctcagcagg ggaaagttct gcatgctgcg ggcccctatc tgcaggatgt
     6961 cggcgtttgc ggccaccaca tctacctgct cgggctccat cacctcggtg atgaccttca
     7021 gcccagtctt ctggccgacc tccgagagga tcttcaaccc ctcttctccc at
//
LOCUS       DATALS010000347         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_260766, whole genome shotgun sequence.
ACCESSION   DATALS010000347 DATALS010000000
VERSION     DATALS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260766"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..2182
                     /locus_tag="VHS06_07755"
     CDS             206..2182
                     /locus_tag="VHS06_07755"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX2988047.1"
                     /translation="MLLCFGGYSPYPLIDWVRQVPGFSSLRVPGRYSMLFTFCIAVLA
                     AYGADWLCKNLRPDVRTAHWHWKARISRSLSVNGFAVYLVGLLASMAFVVWWLLSFRI
                     WIEKEPWAAKRFIETSYLSLRSDKPWLTSDMVLSFLNYSLDPTNPKTTISLALMLATF
                     VLLFCWFAFRRGWRLWASLLVLLVAGDMMLFALDFHPTVHINQLSNPDSAARWLMAQN
                     LDDSSRVYTRKDVRKTEANKLLPFQVADITGYSSLETQRHQEYMGKLAEFEQGLLDLY
                     GVRFVVLPKKITALPSYEYTSYNPNRPLADGPKNNRSAHVTYYMNPPVKADEVSLISS
                     LRSAADIPQDAEVADVVVVDTNGERVTLKVKAGRDTAEWAWDRPDVRPHVGHQQPKVA
                     DKIWASDTEGRRYQANLYYGNLKLDKTRTVQRIEFHYNYPKGSMRLFGMMLLERPNTA
                     HQVLGRDRLIPRYEDDEVQILENPARLPRTYLVPTARVMKRGDILDTMANGDFDSQRV
                     VLLESERKSSNPLPDAGIADATEIDRWLKGNSDTMAGSARILSDSSNEVAIQTESDRD
                     SMLFLADSYYPGWKALVDGQEQRVYRADYLFRAVQVPKGKHEVRFVFEPASFDLGRDI
                     SLYTLGVLLVVLTGLLSAPLLAMLWRRLRRPRRR"
BASE COUNT          502 a          816 c          824 g          544 t
ORIGIN      
        1 gcaaccggcc ggttctggaa ggggtgcagc ggatcgtcgt tcagcttctt ccaatcggcg
       61 ccggagatgc cggtactaaa aaggttgggg tcgaatcgag ggtagctcgc cagcgcaagg
      121 acgctattgt cctgtacgtt cagcatgacc acgctgccgg cctgggaccc caacgactcc
      181 tcagccagct tttgcgacct tcagcatgct gctttgcttc ggcggttact cgccataccc
      241 tctgatcgac tgggttaggc aggtgcccgg cttctcgtcg ctgcgggttc cgggccgcta
      301 tagcatgctg ttcaccttct gcatcgcggt gctggccgcc tacggggccg attggctctg
      361 caaaaacttg cggccggatg tccggacggc gcactggcac tggaaggcgc gaatctctag
      421 atcgctgagt gtcaacggct ttgccgtcta tctggttggg ttgctcgcct ccatggcctt
      481 cgtcgtgtgg tggctgctca gtttccgcat ctggatcgag aaggagccct gggcagccaa
      541 acggttcata gaaaccagtt acctatcctt gcgcagcgac aagccgtggc tcacctcgga
      601 tatggtcctc tctttcctga actactccct ggacccgacc aatcccaaaa cgacgatctc
      661 gctggcactg atgcttgcca ccttcgttct gctgttctgc tggttcgctt ttaggcgcgg
      721 gtggcggttg tgggcctccc tgctggtatt gctggtggcg ggcgacatga tgctctttgc
      781 cctggatttc caccccaccg tgcacatcaa ccaattgtcc aaccccgatt ctgccgctcg
      841 ctggctgatg gctcagaacc tcgacgactc ctcccgagtg tacactcgga aggatgttcg
      901 gaagacggag gccaacaagc tgttgccctt ccaggtggcc gatatcaccg gctactcctc
      961 cctggagacc cagcggcatc aggagtacat gggaaagctg gccgagttcg agcaggggtt
     1021 gctggacctc tacggcgtgc gcttcgtggt gttgccgaag aagataactg ctcttccatc
     1081 ctatgagtac acctcataca atcccaaccg accgctggcc gacggcccaa agaacaaccg
     1141 cagcgcccat gtgacctatt acatgaaccc gcccgtgaag gccgacgagg tgtcgttgat
     1201 ctcgagcctt cgcagcgcag cggacatccc tcaggatgca gaagtcgccg acgttgttgt
     1261 ggtggacacc aacggtgagc gggtgacctt gaaggttaag gccggaagag acaccgccga
     1321 gtgggcatgg gaccggccgg atgtaaggcc ccacgtcggc caccaacagc ccaaggtggc
     1381 cgacaagatc tgggcttccg acaccgaagg taggaggtac caggccaacc tctactacgg
     1441 caacttgaag cttgacaaga cgcgcacggt gcagcggata gagttccact acaactaccc
     1501 caagggctcc atgaggttgt tcggcatgat gctgttggaa cgcccgaaca ctgcccatca
     1561 ggtgctaggg agggatcgtc tcatcccacg ctacgaggac gacgaggtac agatcctgga
     1621 gaaccctgcc aggcttcccc gaacctacct ggtgcccacc gcgcgtgtga tgaagcgggg
     1681 ggatatcctg gatactatgg ccaacggcga cttcgattct caacgggttg tgctgttgga
     1741 gagcgagagg aagagctcca atccgcttcc cgacgccggc atcgccgacg ccaccgagat
     1801 agatcggtgg ctcaagggga actccgacac catggctggg agcgctcgta tcctatccga
     1861 cagctcaaac gaggtggcta tccagaccga gagcgaccgg gattccatgc tcttcctggc
     1921 cgacagctac tatccaggct ggaaggcgct ggtggatggc caggagcagc gggtgtatcg
     1981 agccgactac ctcttccgtg ctgtacaggt gccgaagggc aaacacgagg tgcgtttcgt
     2041 cttcgagccc gccagtttcg acctgggcag ggatatctcc ctctacacgc ttggggtgct
     2101 gctggttgtc ttgacgggac tgttgagtgc tccgttgctc gccatgctct ggcgtcggct
     2161 acgccgtccc cgccgacgct gatctggagt gcggcggatg ggcgtcgctt tcagggggag
     2221 gcgtaagccg acccgagccg tgggagtgcg agatgtagta ggccaccgtg tccgtcttgg
     2281 gagcgcaagg agtaatgggc cggcgtgcct atcctgggag cgcgggcgtc tcgcccgcag
     2341 gagggccgaa ggcccgcaca gagaagacga acaggatgta caggatgaga cggatagaca
     2401 ggatttgggc gaatcatttc ggttctcatc ctgtatatcc gggatatcct gtctatcctg
     2461 tttgaatcgc ttccccacgc gtggccatat cacgctgatg cgggcaagcc gcctgcatcg
     2521 ccgtctcgta gggcggggtc ttgtgccccg cctcccgcgg tgcggccacg accagcacgg
     2581 tgattacgac tgcgcagacg gcggggcaca agcgcgaccg cgacgccacg ctcgctaccg
     2641 tcgcgtgctc gctcgcgtca cgtgccctac gtctcaatcc cgccta
//
LOCUS       DATALS010000348         7749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_220999, whole genome shotgun sequence.
ACCESSION   DATALS010000348 DATALS010000000
VERSION     DATALS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220999"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VHS06_07760"
     CDS             complement(<1..1211)
                     /locus_tag="VHS06_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2988048.1"
                     /translation="MSESNGKSQWNEKTFSPAVKRFPQRQEQFYTTSEIPVEALYSPE
                     NLEGFDYQEQLGYPGEFPYTRGAQPSMYRGRLWTMRQYAGFADAEESNRRYRYLLEQG
                     QTGLSVAFDLPTQIGYDSDSPLSEGEVGRVGVAIDSIEDMETLFKDIPLNKVSTSMTI
                     NATAPILLAMYVVAAKRQGVPLESISGTIQNDVLKEYIARGTYIFPPKPAMKLVTDTF
                     AYCTQKLPKWNFINVSGYHIREAGSNAIQELAFTMANAVAYVRAGLEAGIDIDKLGPQ
                     FAFFFVSHNNLFEEVAKFRAARRMWAHMMRDRFGAKNPKSWVLRFHTQTAGSTLTAQQ
                     PENNVVRVAIQALAAVLGGTQSLHTCARDEALALPTVQSAQIALRTQQLLAFESVSMS
                     SMESMATPTRPT"
     gene            1910..2935
                     /locus_tag="VHS06_07765"
     CDS             1910..2935
                     /locus_tag="VHS06_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX2988049.1"
                     /translation="MPAVQEFARTFRSMNTDVIAIVNAGLHRQAEVEQALAEVQALFE
                     ECEDVMSRFRPGSELSWLNRSSGQPFVASPLLFQVVSDAISWSYATGGVFDPTVLRAI
                     VDAGYDRSFEELPTVPVGESSSTGRERNATIRGNRLSERPWQTAGSGGHTTLSVSAWT
                     RIRLDRSTRTITMPPEVGIDLGGIGKGWAVDRAVDRLRAFDNFAVDAGGDLYVGGTLS
                     DGGPWTVGVQDPFFLEHDMLTLVARDRAVATSTTMRRRWALNGEPQHHLIDPRTGHPS
                     ASGVASVTVLAESVASAEVVAKVALLMGPEAGQRFLERRSVDWVMVTVEGMLKRSPGI
                     EGALYEP"
     gene            2925..4046
                     /locus_tag="VHS06_07770"
     CDS             2925..4046
                     /locus_tag="VHS06_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988050.1"
                     /translation="MSRRQLVAALGTLLVIMCGVGLFWRPTPSAAAQSVRLDGSARIS
                     QGRGATRLVLSATSGDASGLDLEASLDMSATQGMPPRSQTGRLTALNDRERGEGDDSE
                     YRGRGVPGSTGPELVVPTQAGGRPQVDLSGVPDRAQISGGQSSTQPGQSTVAAVSVGG
                     TRTGDPTFWHIARAAGLSAYLMLFLNVFLGLAVHTHYLEGVMARWRSFDLHQFTALLA
                     IGFLGLHALALLGDRYMGFTPAQLVIPFASPYRPFWTALGVVGAYAVLIVFGSSYVRQ
                     QIGYRAWRAIHYVAFAAFLLTMVHGVFSGTDSGEPWAAALYAGTGATIALLTLMRFKG
                     VAEQRTPAAPKLPVLARSERVPVEVGADRMAEGDGVRTV"
     gene            complement(4140..4427)
                     /locus_tag="VHS06_07775"
     CDS             complement(4140..4427)
                     /locus_tag="VHS06_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988051.1"
                     /translation="MQRLSIIRWQQTQGKYVAILQAQERFKAETLKARPIIKGATVLE
                     GELVGTNQKEWYCISFPKEDWEFEEVREQAKTLLDMEYQRRAAGKPSLTAG"
     gene            4684..5142
                     /locus_tag="VHS06_07780"
     CDS             4684..5142
                     /locus_tag="VHS06_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988052.1"
                     /translation="MSNRGGWAEHDEERPLAEESLVDAFYRVFKQIVSALDVPFGYTL
                     SIWASGQLAVSRYGQPDLGDVLLFVGGAVSAYILMGVLASLRSTVQGVRRGTGVTVLN
                     VIALLVTVVVAAVASAVDFPPAGFYLTGLLATMIYSLLLTVLILIGTRVG"
     gene            complement(5187..5687)
                     /locus_tag="VHS06_07785"
     CDS             complement(5187..5687)
                     /locus_tag="VHS06_07785"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HEX2988053.1"
                     /translation="MSDAIDRLDSLSLGYSLQERPLGSYALVVGSFSFSIGAFLLLMN
                     RVGKALPPSLNWWDLLLMGTATQRAARLISRDEATSFIRAPFTELVGPGLVQGEVTEK
                     PRGKGLRRVIGELLTSPYSVSQWVAAVFICGLVAVPRVTRFLAAILTTSAISDLLRRR
                     LDTGQS"
     gene            5848..7680
                     /locus_tag="VHS06_07790"
     CDS             5848..7680
                     /locus_tag="VHS06_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX2988054.1"
                     /translation="MFQRELDLAVSSGERMWHHLNRAAEAPRIGDVTNELRAALDQAW
                     RMESALGVFLSIEEPKLRAAQLVGSAAVAGSAFDSLKAALVDADEARQRDEAEMKSWI
                     DGFRPHAQRALEMAKQAQASMPPQGSHRISAPLGEATLDRAQAVVSIARTGRQNLSVG
                     DVKELVRQALEPMGGIGRFVGQGQTVLLKPNQTLFLLSDEGSTTDPRVVAALTHLSFE
                     AGAARVIVGESSGGGSKSDQVMHVTGVKQLALRAGAEVVDFQVCEQREVEIPDGKLIK
                     RILLPVPLLDADVVINLPKMKTHNWDWISGALKNWVGIVRPDVREQHHDAGTFDEYVD
                     LHFRVPAQLHVMDAIVRGVGNGPGANTGEFYGGILASTDPVAMDAVAAQILGFDPASI
                     GFVLVAMERGLGIGNPNEIAVVGVPLREAFKPGRPPEMGVDLFDANVIVGRGLTRSGT
                     LGHFKSMGDIFQQMGVWNVVRRLHGRPTILIGDAEDPLFTQHLLEGPYLVIDDAAPEK
                     YRQHPEVMHVPGHPVLHNLESELPIGLRIPRLARVAMGVMGEARTLDARLEFDSPQPL
                     VPLVRGAIGITRLLPVAAQVALTVGVTGMLLLGLGLGVRRLITD"
BASE COUNT         1338 a         2310 c         2535 g         1566 t
ORIGIN      
        1 gtcggccgag tgggtgtggc catcgactcc atcgaggaca tggagacgct ctcgaaggcc
       61 agcagttgct gcgtgcggag ggcgatctgg gccgactgca cggtggggag tgccagcgcc
      121 tcgtcgcgtg cgcaggtgtg caacgattgg gtgccaccca acaccgccgc caacgcctgg
      181 atcgccacgc ggaccacgtt gttctcgggc tgctgggcgg ttagggtgct tcctgcggtc
      241 tgggtgtgga agcgaaggac ccacgacttc gggttcttgg cgccgaagcg atcgcgcatc
      301 atgtgggccc acatccttct ggcggcccgg aattttgcga cctcttcgaa caggttgttg
      361 tgggagacga agaagaaggc gaactgtggg ccgagtttgt cgatgtcgat gccggcttcg
      421 agcccggcgc gaacgtaggc caccgcgttg gccatggtga aggccagttc ctggatcgcg
      481 ttggagcccg cctcgcggat gtggtagccg ctgacgttga tgaagttcca cttcggcagc
      541 ttctgggtgc agtaggcgaa ggtgtcggtc accaacttca tcgccggctt cggcgggaag
      601 atgtaggtgc cgcgggcgat gtactccttc aggacgtcgt tctgaatcgt gccggagatg
      661 gactccaggg gaacgccctg ccgtttcgcc gcgaccacgt acatggccag caggatgggg
      721 gcggtggcgt tgatggtcat cgaggtgctg accttgttca gcgggatgtc cttgaagagc
      781 gtctccatgt cctcgatgga gtcgatggcc acacccactc ggccgacctc gccctcggac
      841 agagggctat cggagtcgta gccgatctgg gtgggcaggt cgaaggcgac gctcaggccg
      901 gtctggccct gctccagcag gtagcggtac cgtctgttgg attcctcggc atcggcgaaa
      961 ccggcgtact gccgcatcgt ccagagccgg ccgcggtaca tggatggctg cgcgccccgg
     1021 gtgtagggaa actcgccggg atacccgagc tgctcctggt agtcgaaacc ctcaaggttc
     1081 tccggagaat acagagcctc gaccgggatc tcggaggtgg tgtagaactg ctcctgcctc
     1141 tgcgggaacc gtttcacagc tgggctaaag gtcttctcgt tccactggga cttgccgttt
     1201 gactcggaca tttttctccc ctgcgctgca agcgttgaaa agttcggcct gcatcagccg
     1261 gacaggaagg accggcggcc acgcagcgaa gaatggagtc tctgctgcgg atctgggcca
     1321 cgctgcctgt gtccgctctg tttgcatttt attgcgcaaa ctctccacaa acaaggtata
     1381 cggcattgcg acttcatatc agttcgcgtt gtggcccgcc ggccggcact cgcctcagcg
     1441 ttcaggccat ccgggggaca tcgataacgc ctgcccctga gcccatctgc cgtcgcctca
     1501 cgacctcccg acgcgtcgac aaactaagca aatattctac agatatttag cacgttttta
     1561 aggctatttc ggcaggcatg gcctaccata tgtcgtgtag acgctgatag cgaatcagga
     1621 ggtaacaata tacagatggc agcgtcaaat cggacatact ggccggcgcg actggcggta
     1681 ctgctgggcg gtgctctggc agcggtcggc ttctggactg gcgtggtcac caaacccgac
     1741 gccgttgccg cggcggatcc acctccccaa gcagtgcaaa gtgccagttc cccaccttct
     1801 tcgcgagggc tctccccact cgccgaatcc caagcgcggg gccaggtcac cccgcagcag
     1861 ccacgaagct cgacatctcc aagactccgg accagggggt cataggccga tgcctgctgt
     1921 ccaggaattt gcgcggacct ttcggtccat gaataccgac gtgatcgcca ttgtcaatgc
     1981 cggcctccac cgacaggcgg aggtcgagca ggcgctggcc gaagtgcagg cactcttcga
     2041 agagtgcgaa gacgtcatga gccgttttcg acctgggagc gagttatcgt ggctcaaccg
     2101 ttcctcgggg cagcccttcg tcgcctctcc tctgctgttt caggttgtct cggacgcgat
     2161 ttcctggtcc tatgcgaccg ggggcgtctt cgatcccacg gtgctcaggg ccatagtcga
     2221 cgcgggctac gaccgcagtt tcgaggagtt gcccacggtt ccggtaggcg aatcctcgtc
     2281 caccggtcgg gagagaaatg ccaccatccg agggaaccgc ttgagcgagc gcccctggca
     2341 gactgccgga tccggtgggc acactactct gtctgtttca gcctggacac gaattcgtct
     2401 ggaccgttcg acgaggacga taaccatgcc gccggaagtc ggtatcgact tgggaggcat
     2461 cggcaagggg tgggcggtag accgagccgt ggatcggcta cgcgcttttg acaacttcgc
     2521 ggtggatgca gggggcgatc tctatgtcgg cggcaccctg tcggatggcg gcccctggac
     2581 ggttggggta caggatccgt tcttcctcga gcatgacatg ttgacactgg ttgccaggga
     2641 ccgggcagtg gcgacctcga ctaccatgcg gcgaaggtgg gccttgaatg gggaacccca
     2701 gcaccacctg atagaccctc gaactgggca cccctccgcg agcggtgtgg cgtcggtcac
     2761 cgttctggcc gagtccgtcg catcggcaga agtagtggcc aaggtggccc tgttgatggg
     2821 cccggaggcc ggacaacggt tcctggagcg gcgatcggtg gattgggtga tggtgaccgt
     2881 ggagggaatg ctgaagcgat cgccagggat agagggggcg ctttatgagc cgtaggcagt
     2941 tggtggcagc cctggggacc ctactcgtca taatgtgtgg ggttgggctg ttctggcgcc
     3001 cgactccctc agcggctgct cagtccgttc gcctggatgg cagcgcacgg atcagccagg
     3061 gccgtggggc gaccagactc gtcctgtcgg ctacctccgg cgacgcatcg ggcctcgacc
     3121 tggaggcatc gctggacatg agcgccacgc aggggatgcc tcccagaagt cagacgggca
     3181 gactgacagc gctgaacgac cgggaacgtg gcgagggcga cgacagtgag tatcggggac
     3241 ggggcgttcc gggatcaacc ggtcccgagt tggtcgtccc cacccaggcc ggcggccggc
     3301 cgcaggtcga tctgtcgggc gtgccggatc gcgcgcagat ttccggtggc caatcgtcga
     3361 cgcagcccgg gcagtcgacc gtggcggccg tatcggtagg ggggacgagg acgggcgacc
     3421 ccaccttctg gcacatagcc cgagcggcgg ggttgagcgc ctacctgatg ctcttcttga
     3481 acgtcttcct gggactcgcc gtgcataccc actatctaga gggggtgatg gctcggtggc
     3541 gcagcttcga ccttcaccag ttcaccgcgt tgctggccat cggctttctc ggcctgcacg
     3601 ccctggcgct gctgggtgat cgctacatgg gcttcactcc cgcgcagttg gtgatcccat
     3661 tcgcatcgcc ctatcggccg ttctggacgg cattgggagt cgtcggcgct tatgctgtgt
     3721 tgattgtgtt cggcagttcc tacgtgcggc aacagatcgg ctatcgggcc tggcgggcga
     3781 tccactatgt ggctttcgcg gcgttcctgc tcaccatggt gcatggggtc ttctccggga
     3841 ccgacagcgg ggagccttgg gcagcggcgc tgtatgcggg aaccggtgcg accatagcgc
     3901 tgctgacgct gatgcgcttc aagggcgtag cggaacagcg cactcccgcg gcaccgaagc
     3961 tgccggtact ggcgcggtct gagcgagtcc cagtggaggt tggagccgat aggatggccg
     4021 agggggacgg agtgcggact gtctgaggcc cccgtctcgc ggctgcgacc tgtgcgtcgc
     4081 cctgcccggg agtcttgtct aaccggcggg cgacgtacac gtcgcccgca tccccacact
     4141 catcctgccg tcagcgacgg cttgcccgcg gctctgcgct ggtattccat gtccagcagc
     4201 gtcttggcct gctcgcggac ctcctcgaac tcccaatcct ccttggggaa gctgatgcag
     4261 taccactcct tctggttggt gcccacgagt tccccttcca ggaccgttgc gcccttgatg
     4321 attggtcggg ccttcagcgt ttcggccttg aaccgctcct gggcctgcag gatggcgacg
     4381 tacttccctt gcgtctgctg ccagcgaatg atcgatagtc tctgcaccga tacctctcct
     4441 gcggcagtat tccccttgat tgtaccctat cctggattga gtctcccacc atctcggcat
     4501 tttgccggtc aggcggttac gtgatgccaa ctcactatcc cacgttccat tgagcggcct
     4561 gtctcttgcg ggtccgctgt ctctagacaa gtggaaagat accgagtcta gtgctatcga
     4621 tcctttcttg atcgcaggct ttatctcgat gatcgcacga ccaacggctg atggatcagt
     4681 attatgagta accgcggggg atgggctgag catgacgagg agcggccgct ggcggaggag
     4741 agcctcgtcg atgctttcta ccgtgttttc aagcagatcg tctctgcact ggatgtccct
     4801 tttggctata ccctctccat ctgggcgtcc ggacagttgg ccgtcagcag gtacgggcaa
     4861 cctgaccttg gtgacgtcct gctgttcgtg ggaggtgcgg tgtctgccta catcctgatg
     4921 ggcgtgctgg cttcccttcg ttcaactgtt cagggtgtga gacggggcac gggagtgact
     4981 gtgttgaacg tgattgcgct cttggtgacc gtggttgtgg ccgctgttgc cagcgcagtg
     5041 gacttcccgc cggcagggtt ctacctgaca ggtttgcttg ccaccatgat ttactccttg
     5101 ctactaactg tcctcatctt gatcggaacg cgtgttgggt gaaccggcac caatcagcgt
     5161 gcgaccatca taggtatggg cggctatcag ctttggccag tgtccagcct tcgccgcagt
     5221 agatccgaaa tcgccgacgt cgtcaggata gctgccagaa accgggtgac gcggggtaca
     5281 gcgacgaggc cgcatatgaa taccgcggcc acccactggc taactgaata agggctcgtc
     5341 agcaactcgc cgatcaccct acgcagccct ttcccccgcg gcttttcggt gacctctccc
     5401 tgcacgagac cggggcctac tagttcggtg aagggggcac gaatgaagct ggttgcctca
     5461 tccctggata tcagtcgggc agccctctga gtcgcggtgc ccatcaggag tagatcccac
     5521 cagttgaggg agggcggcag ggccttgcca actcggttca tgaggaggag aaaggcaccg
     5581 atggagaagg agaagcttcc caccaccagg gcgtatgaac ccagagggcg ctcttggagg
     5641 ctgtagccca aagataggct gtctagccta tcgatcgcgt cgctcatcgc cctctttccc
     5701 cctttgtttc gatcgaggct ggggcgcaag agtcatgccc tttttcccta caccctcctc
     5761 ttcctcgcgc gcgtttccac gaggaagaag gggcacgtat cctgccggac tggtcgatag
     5821 gattgtgatg tgacaggagg ggtgggcatg ttccagcgag agctcgatct agccgtctca
     5881 tcgggcgagc gaatgtggca tcacctgaac agggcagccg aggcaccgcg cataggcgac
     5941 gtgaccaacg aactgcgggc agccttggac caggcatggc ggatggagtc cgcgctcggc
     6001 gtgtttctat caatagagga accgaagctg agggcagctc agctggtggg gtccgcggct
     6061 gtggccggat ccgccttcga tagcctcaag gctgcccttg tggatgcgga cgaggcccgt
     6121 cagagggacg aggccgagat gaagagctgg atcgacggct ttcggccaca cgcgcagagg
     6181 gccctcgaaa tggccaagca ggcccaggca tcgatgccgc cccagggctc tcatcggata
     6241 tcggctccgc taggagaggc gaccctggat cgagcccagg cagtcgtctc catcgcccgc
     6301 acggggaggc agaacctgag cgtgggggac gtgaaggagt tggtccggca ggccctggag
     6361 ccgatgggcg ggataggccg cttcgtcgga caggggcaga ccgttctgct caagccgaac
     6421 cagacgctgt tcctgctttc tgacgagggc tccacgaccg accccagggt ggtggcggcc
     6481 ctcactcacc tcagtttcga ggccggcgcc gccagggtga tcgtcgggga gtcctccggt
     6541 ggcggatcaa agagcgacca ggttatgcac gtcaccggcg tcaagcagtt ggcccttcgg
     6601 gccggtgccg aggtggtgga cttccaggtc tgcgaacagc gcgaggtgga gatccccgac
     6661 ggcaaactga tcaaacggat cctcttaccc gtccccctgc ttgatgccga cgtcgtgatc
     6721 aaccttccca agatgaagac ccacaactgg gattggattt cgggggcgtt gaagaattgg
     6781 gttgggatcg ttcgccccga cgtgcgcgag caacatcacg acgccggaac cttcgacgag
     6841 tacgtcgatc tccatttcag ggtgccggca cagcttcacg tcatggatgc tatcgtccgt
     6901 ggggtcggaa atggaccggg agccaacaca ggggagttct acggcggcat tctcgccagc
     6961 accgatcccg tcgcgatgga tgcggtggcg gcccagatcc tcggcttcga tcccgcttca
     7021 atcggcttcg ttctggtggc tatggagagg ggactgggca tcggaaatcc caatgagatc
     7081 gcggtggttg gggttcccct ccgggaggca ttcaaacccg ggcgaccgcc ggagatggga
     7141 gtcgatctct tcgacgccaa tgtcatcgtg ggccggggct tgacccgctc cggcactctc
     7201 ggccacttca agtccatggg cgacatcttt cagcagatgg gtgtgtggaa cgtggttcgg
     7261 cggcttcacg gaaggccgac gatcttgatc ggcgacgcgg aggatcccct cttcacccaa
     7321 cacctgctgg aggggccata cctggtgatc gacgacgcgg cgccggagaa gtacaggcaa
     7381 catcccgaag ttatgcacgt tcccgggcac ccggtgctac acaacctgga gtcggagcta
     7441 cccatcgggc ttcggatccc aaggctggca agggtggcga tgggtgtcat gggtgaggcg
     7501 cgcacgctgg acgctcgcct ggagtttgac tcgcctcagc cgctggttcc gttggtccgt
     7561 ggagccatag ggatcacgag actgctaccg gttgcggcgc aggttgccct gaccgtcgga
     7621 gttaccggca tgttgctgct gggtctcggc ctaggcgtga ggaggctgat caccgactag
     7681 aaccgttctc tcctactacc tagctggccc caaggttgta gacggtgcga ggactaatct
     7741 ttccggcca
//
LOCUS       DATALS010000349         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_37621,
            whole genome shotgun sequence.
ACCESSION   DATALS010000349 DATALS010000000
VERSION     DATALS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37621"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VHS06_07795"
     CDS             complement(<1..410)
                     /locus_tag="VHS06_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX2988055.1"
                     /translation="MPDARPNLLLITSDQQHWNTLGCLNPEIATPSLDRLAREGMMFG
                     RAYCPNPTCTPTRASIITGQYPSQHGAWSLGTKLPETAHTVGEDLQAAGYRTALIGKA
                     HFQQLLGTEEYPSLEANAVPPSGHTMHDLGFWRE"
     gene            857..>1555
                     /locus_tag="VHS06_07800"
     CDS             857..>1555
                     /locus_tag="VHS06_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988056.1"
                     /translation="MGYQQSAPATPLALSLCPAAPSRQGAPPPAPKPPSTMGYDLGRA
                     ALNLQWTPRVARTEYVDNWEIVRHFTGKDPQTQPEAWREFYDAIHLDYLWTTHDGPIS
                     WEQRGRVTDMGHAVFVEDGSDYHLPTPSPFRDVEEVLAFDAVQEYGLVPDLNALTETY
                     EHWYHRRQAECDQVISGGYYRTLISGAIAAFGWDLLLEAAGTDPEHFGERVLGSIFNV
                     TKHHVQAWARTSIEV"
BASE COUNT          257 a          572 c          470 g          256 t
ORIGIN      
        1 aactcccgcc agaaccccag gtcgtgcatg gtatgcccgc tcggcggcac cgcgttggcc
       61 tccagcgacg ggtactcctc tgtccccagc aactgctgaa agtgcgcctt cccgatcagc
      121 gcggtgcgat accccgccgc ctgcaagtcc tcgcccaccg tatgcgccgt ctccggcagc
      181 ttcgtcccca atgaccacgc cccgtgctga ctcggatact gccccgtaat gatggaagcc
      241 cgcgtcggcg tacacgtcgg attggggcaa taggcccgcc cgaacatcat gccctctcgc
      301 gccagtcgat cgagcgaagg cgtagcgatc tcggggttca ggcatcccag cgtattccag
      361 tgctgctgat cggaagtgat gaggaggaga ttcggtctgg cgtctggcac aggcagtgac
      421 ctcagtgatg aatgctgggc ccagtttcgc cgcccacgct ccgcctcccg ccatcgcgtg
      481 tggcacggcc tctagctggc cccgtagttg ggcggaacca gcccgtggcc ctcccgcccc
      541 tccagtggcg caatccgcta gccgaaggat tgtggcggca gcgaagccgc tgaagatgta
      601 gggcgggcat gtcatgcagt tcgcatgccc gccgccagta accacgccgc atgtcacccc
      661 cgaccgtccc tcccagggtc gcgccccgct gtcatcctga gtgtggcggc gaagccgcca
      721 cccgtgagaa cacgaaggac ccaaccggcg atggtgcgaa gcccatcgcc agtaccatcg
      781 gcgaagccac tcctgcgtgg atgtcattcc gacttgcccg gtgcagttcc gggcgtagtg
      841 gcgaggaatc tagtcgttgg gctatcagca atcggcgcca gccactcctc ttgcgctgtc
      901 gctgtgccct gctgccccct cccggcaggg cgccccacct ccggcgccga agcccccctc
      961 caccatgggc tacgacctcg gccgcgccgc gctcaacctc cagtggaccc cccgcgtcgc
     1021 gcgcacggaa tacgtggaca actgggagat cgtccgccac ttcaccggca aggacccgca
     1081 gacccagccg gaggcctggc gcgagttcta cgacgccata cacctcgact acctctggac
     1141 cacccacgac ggcccgatct cgtgggagca gcggggccgg gtgaccgaca tgggtcacgc
     1201 cgtgttcgtg gaggatggct ccgactacca tctcccaacg ccctcgccgt ttcgggacgt
     1261 ggaggaggtc ctcgccttcg acgccgtgca ggagtacggg ctggtccctg acctgaacgc
     1321 gctcacggag acgtacgagc actggtatca ccgccggcag gccgagtgcg accaggtcat
     1381 ctccggcggg tactaccgca ccctcatctc cggtgccatc gccgccttcg gttgggacct
     1441 gctgctggag gccgcgggca ccgacccgga gcacttcggc gaacgcgtcc tcggctccat
     1501 cttcaacgta acgaagcacc acgtgcaggc ctgggcacgc acctccatcg aggtc
//
LOCUS       DATALS010000350         4946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_38073,
            whole genome shotgun sequence.
ACCESSION   DATALS010000350 DATALS010000000
VERSION     DATALS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38073"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VHS06_07805"
     CDS             <1..529
                     /locus_tag="VHS06_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX2988057.1"
                     /translation="AASHQVRSALREGLLRVAAPVISQEGRTQMVALVYIATDSVQQA
                     IRDEILRALLISLAAILVGVLISILFSRGITRPVAILTDAASALEADNPFNSERLVAL
                     AANGDELGKMARVFARMAKEIHVREDRLKSQVAQLKIEIDEAKRARQVQEITDTDYFQ
                     NLRGRAQQLRTRKDE"
     gene            529..1302
                     /locus_tag="VHS06_07810"
     CDS             529..1302
                     /locus_tag="VHS06_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX2988058.1"
                     /translation="MARIVSIHSFRGGTGKSNTTANLTALLAQAGYRVGVIDTDIQSP
                     GIHVLFGLDEEKMDRSLNDYLWGKCAIEDAAYDVSPLVRMGKGAAAVMGGGIHLIPSS
                     IKAGEIARVLQEGYDVNLLSDGFRELTKRLKLDYLLIDTHPGLNEETLLSIALSDELI
                     IILRPDQQDYQGTAVTVDVARKLDVPRMMLVVNKVLSSFDFDAVKTRVEQSYDVPVAG
                     VLPLTEEMVALGSSDLFALRYPDHPLTQRLRAIVEQVMA"
     gene            1313..1474
                     /locus_tag="VHS06_07815"
     CDS             1313..1474
                     /locus_tag="VHS06_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988059.1"
                     /translation="MGVFDRLQDEMVAREKAAGLTMADVLSLPDPERRLFNWVMRRGR
                     QIRPKWSRG"
     gene            1459..1662
                     /locus_tag="VHS06_07820"
     CDS             1459..1662
                     /locus_tag="VHS06_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988060.1"
                     /translation="MVEGMRLTEAEVDTLLSALVEKGYLRELAYSGGSRYKPRLASKR
                     GSELSTDLWHALSDRTEEERRQS"
     gene            1659..1871
                     /locus_tag="VHS06_07825"
     CDS             1659..1871
                     /locus_tag="VHS06_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988061.1"
                     /translation="MNCFHCEKPAQGVCRFCGRALCKDHMQKMPYIITIYVGDSNTPK
                     SIVVADALFCGVCKPQPEPIEMPELY"
     gene            2140..3750
                     /locus_tag="VHS06_07830"
     CDS             2140..3750
                     /locus_tag="VHS06_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988062.1"
                     /translation="MAVQAVEPGLQPLRRGLQRAQQRLWLQILVRTAARGICVGLALT
                     LVAALLTGWQAPAEILSGLWWASILCPVAGIIAALFLKPSQARAARVTDLQLQLHQQL
                     GTAEELLSRNADGTLVSMQLASAASVADEISVARAFPLVPRREMAVALLLAILTAGVF
                     GLANAGIILPNPFAAIRLPSLTQRTDSRPDQKLFSQQQANPTQTQSPALESFRQSMDQ
                     IQKQSLLGKLSGNAASALLSQAGAELNRVAAQSQAQQQALDALSSQLRNTAAGKDAGE
                     SLRQGDAKQAAQQLQQLGQQSDQLSDTAKQELADALNQAAQNSQASPNLAKAENRAAQ
                     QLQSGDYSDVTQSMDQLAQSVQDAANQTISQSELAQSWQQLQQLSNNLGQGNPAGSKD
                     SPPVAQASPATGERTAEGQQGAPAQGAPAAPQGQQGTDQPNSQPGGPGTPGNQRGGPA
                     VGNRNPRLGPDGKPLDVQGSVSGQFSDQQSNSPQPPSVTRQGTGNSISAGGDGANGPT
                     SVPAENVFVPGERRPTVRDYFSKGSGNQ"
     gene            3747..4751
                     /locus_tag="VHS06_07835"
     CDS             3747..4751
                     /locus_tag="VHS06_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2988063.1"
                     /translation="MIEANPSVDRLSVDQLKETVKGIEEEIGRFIVGQRDTVRGVITC
                     LLCGGHALLEGVPGLGKTMMVRTLADALELQHGRIQFTPDLMPSDIVGTNILVDDEDG
                     QKRLTFRPGPVFANLVLADEINRATPKTQSALLEAMQEQGISVGNHTYALPKPFFVMA
                     TQNPIEMEGTYPLPEAQLDRFFFKLEVGYPSSEEMMQILQRTTSADSYQPSHIADADW
                     LIQAQALVRDVPIASYVLEYAVRLTMATHPDQPTALPIVKQYLRYGASPRAAQTLVLA
                     GKVGALLEGRFNVSYRDIRQAAKPALRHRIIPNLEAELARVTPDAIIDELVQRVPEEV
                     "
     gene            4751..>4946
                     /locus_tag="VHS06_07840"
     CDS             4751..>4946
                     /locus_tag="VHS06_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988064.1"
                     /translation="MLFDRLFGRKRWSGAPLPGPARHSADITGNAVDDGFLESLRQVS
                     LDSRPRMTGGHTGEHASPRRA"
BASE COUNT         1023 a         1579 c         1549 g          795 t
ORIGIN      
        1 tgccgcctct caccaggtca ggtcggcgct gcgggaaggt ctcctgaggg tggcagctcc
       61 ggtcattagc caggagggga ggacccagat ggtcgccctg gtctacatcg ccaccgacag
      121 cgtgcagcag gctattcggg acgaaatcct gcgcgcgctt ctcatctctc ttgcagcgat
      181 cctcgtagga gtgctgatct ccattctgtt ctctcgcggg atcaccaggc cagtcgccat
      241 cctcaccgat gcggcatccg cgctggaagc cgacaacccc ttcaactcgg agaggctggt
      301 tgccctggca gcgaacggcg acgagttggg caagatggcc agggtcttcg cccgcatggc
      361 aaaggagatc catgtcaggg aggatcggct gaagagtcag gttgcccagt tgaagatcga
      421 gatcgacgag gccaaaaggg cccgtcaggt ccaggaaatc acggacacgg actacttcca
      481 gaatctccgc ggccgcgccc aacagttgcg gactagaaag gacgaatgat ggccaggatc
      541 gtatccatac actccttccg gggcggtacc ggcaaaagca acaccaccgc caatctcact
      601 gctctccttg cccaggcagg ttacagggtg ggagtcatcg acaccgacat ccaatcgccg
      661 gggatccacg tcctcttcgg cctcgacgag gaaaagatgg acaggtcgct gaacgactac
      721 ctgtggggca agtgcgccat cgaggatgcc gcctacgacg tgagccccct cgtccggatg
      781 gggaagggcg cggcggcagt gatgggtgga ggcatccacc tgatcccctc ctccatcaag
      841 gccggagaga tcgccagggt gctacaggag ggctacgacg tgaacctcct gtccgacggt
      901 ttcagggagt tgaccaagag gctgaagctg gactatctgc tgatcgacac acacccgggc
      961 ctgaatgagg aaaccctcct ctccatcgcc ctctccgacg aactgatcat catcctccgg
     1021 cccgaccagc aggactacca gggcacggcc gtcacagtgg acgtggcccg caaactggac
     1081 gttccccgga tgatgctggt cgtcaacaag gttctttctt ccttcgactt cgatgccgtc
     1141 aagactcgcg tggagcagag ctacgacgtt ccggtggcgg gagtgctgcc gttgaccgag
     1201 gagatggttg cgctgggcag cagcgacctc ttcgccctgc gctatccgga tcacccgctg
     1261 acccagaggc tgcgagccat cgtcgagcag gtcatggcgt gaggaggcgg tgatgggagt
     1321 atttgaccgg cttcaggacg agatggtagc ccgagaaaag gctgccggat taaccatggc
     1381 cgacgtcctc tccctgcccg acccggagcg gcggcttttc aactgggtca tgcgccgggg
     1441 gaggcagatc aggccgaaat ggtcgagggg atgaggctca ccgaggctga ggtggacacc
     1501 ctgctgtcgg ccctggtgga gaagggctac ctacgagagc ttgcttacag cggcgggtcc
     1561 cgctacaagc cgcgccttgc ctcgaaacgg ggcagtgagc tctccacgga cctatggcac
     1621 gccctttccg acaggactga agaagagagg aggcaatcat gaactgcttc cactgcgaga
     1681 aacctgccca gggggtgtgc cgcttctgcg gacgagccct ctgcaaagat catatgcaga
     1741 agatgcccta catcatcacc atctacgttg gggatagtaa caccccaaag tccatcgtgg
     1801 tggccgacgc cctcttctgc ggggtgtgca agccccagcc ggagccgata gagatgccgg
     1861 agctgtactg acagcgatcg tggaggtagg cgcgctcgaa atgcaattct gcccaggggt
     1921 gcatgcaccc ctgggcagaa ttgcgaaccc ggccttcttc catctctact ggcaccctta
     1981 ttgctttgcc acgtccaagc ccggtggagt caccagtgac cattctaggc ctcatggtgg
     2041 gccccgcgag caccggttgc agaggcaagc aagaacagcc cgagacagac cggtggcgag
     2101 gcaggtccca agaacggccg ggccggacag gggagatcat tggctgttca ggcggtcgag
     2161 cccggtcttc agccccttcg caggggcctt cagcgagcgc aacaaaggct gtggcttcag
     2221 atactggttc gcactgccgc tcgtggcatc tgcgtcggac tggcattaac cctggtcgcg
     2281 gccctgctga cgggctggca ggcaccggca gagatcctca gcgggttatg gtgggcatcg
     2341 atcctctgcc cggtcgccgg aatcatcgcg gcactctttc tcaagccctc tcaggccagg
     2401 gcggcccgcg tcacggacct ccaattacag ctccatcaac agctcgggac tgccgaggag
     2461 ctgctgtcgc gaaacgccga cggcacactg gtctccatgc agctggcctc cgccgcgtcg
     2521 gtggccgatg agatttccgt cgcgagggct tttcccctgg tacctcgacg ggagatggcc
     2581 gtggcgctgt tgctggcgat cctcaccgcg ggagtgttcg gccttgccaa cgcgggcatc
     2641 atccttccca acccgttcgc cgccatccgc ctaccatctc ttacccaacg gaccgattcc
     2701 cggcccgacc agaagctgtt cagccagcag caagccaacc cgacacaaac ccagtcccct
     2761 gcgctggaaa gcttccgtca gtcgatggac cagatccaga agcagtccct gctcgggaag
     2821 ctctcgggaa atgccgcctc cgccctgctc tcccaggcgg gggccgaact caacagggtc
     2881 gccgcgcagt cccaggccca gcagcaggcg ctggacgccc tctcatctca gcttcgcaac
     2941 acggcggcag gcaaggacgc gggcgagagt ctgcgacagg gggacgccaa gcaggccgca
     3001 cagcagcttc aacaactcgg ccagcagagc gaccagctgt cggatacagc caaacaggaa
     3061 ctggcagatg cgctcaatca agcggcgcag aacagccagg catccccgaa tctggccaag
     3121 gccgagaata gggcggcgca acaactccag agcggcgact actccgacgt cacccagtcc
     3181 atggatcaac tcgcccagtc ggtgcaggac gcggccaatc agaccatttc acagagcgag
     3241 ttggcgcaga gctggcagca actgcagcag ctgagcaaca accttgggca ggggaatccg
     3301 gccggctcca aagattcacc accggtggcg caggcatcgc cggcgaccgg cgagcgtacg
     3361 gcagagggac agcaaggtgc acccgctcag ggggcccccg ccgctcccca gggacagcag
     3421 ggaacagatc agcccaacag ccagccggga gggccgggga caccgggtaa ccagagaggt
     3481 ggaccggccg tggggaatcg gaaccctcgc ctggggcccg acggcaagcc gctggacgtg
     3541 cagggaagcg tatccgggca gttttccgac cagcagtcca acagtccaca accgccatcg
     3601 gtaacccggc aaggcaccgg caactccata tcggccggcg gcgacggggc caacgggccc
     3661 acctcggtgc cggcagagaa cgtgttcgtg cccggtgagc gaaggcctac ggtgcgcgac
     3721 tacttcagta agggttctgg aaaccaatga tagaagccaa tccttcggta gaccggctct
     3781 cggtagacca gctcaaagaa accgtgaagg gaatagagga ggagatcggt cggttcatcg
     3841 tcggacagag ggacacggtg aggggtgtca taacctgcct tctgtgcggt ggccacgccc
     3901 ttctggaagg tgttcccggc ctgggcaaga cgatgatggt gcgcaccctg gcggacgcac
     3961 tggaactgca gcacggccgc atccagttca caccggacct gatgcccagc gacatagtgg
     4021 gtaccaacat cctggtggac gatgaggatg gacagaagcg acttaccttc aggccgggcc
     4081 cggtcttcgc caacctggtg ctggccgacg agatcaacag ggcgacccca aaaacccaga
     4141 gcgccctgct cgaggcgatg caggagcagg ggatcagcgt gggcaatcac acctatgcgc
     4201 ttcccaaacc gttcttcgtg atggcgactc agaaccccat cgagatggag gggacctacc
     4261 cactgcccga agcacagctg gaccgtttct tcttcaagct cgaggtgggt tacccgtcgt
     4321 ccgaggagat gatgcagatc ctgcaacgca ccaccagcgc tgatagctac cagccaagcc
     4381 acatcgccga cgccgactgg ctgatccaag cccaggcact ggtgagagac gttcccatcg
     4441 ccagctacgt tctggaatac gcggtgcgtc tcaccatggc gacacacccg gatcaaccca
     4501 cggcgctgcc catcgtcaag cagtacctac gctacggtgc aagtccgagg gccgcgcaga
     4561 ccctggtact ggcggggaag gtgggagcgc tgctggaagg tcgcttcaac gtctcgtacc
     4621 gtgacatccg gcaggctgcc aagccggccc tcaggcatcg gatcatcccg aacctggagg
     4681 ctgagctggc cagggtgacc cccgacgcaa tcatcgacga gttggttcag cgggttccgg
     4741 aggaggtctg atgctcttcg acagactgtt tggtcgaaaa cgatggtccg gggccccact
     4801 gccgggcccc gccagacact ccgcggacat cacggggaac gccgtggacg acggcttcct
     4861 ggaatcgctc cggcaggtga gcctcgactc ccgaccgcgg atgaccggcg gccacaccgg
     4921 ggagcacgct agcccgagac gagcca
//
LOCUS       DATALS010000351         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_38291,
            whole genome shotgun sequence.
ACCESSION   DATALS010000351 DATALS010000000
VERSION     DATALS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38291"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..536)
                     /locus_tag="VHS06_07845"
     CDS             complement(120..536)
                     /locus_tag="VHS06_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX2988065.1"
                     /translation="MSTAAHGCELAQVLKELGTDANTGLTSEEARRRLERYGPNELKE
                     KPRPGFLQLLWGQVNNFLIILLIVASVVSAALGELTDAGMIMTIVVLNAVLGVVQESK
                     AEAAMAALKKMAAPNARVIRDDSIQDVPSRERHPRY"
     gene            complement(547..1005)
                     /locus_tag="VHS06_07850"
     CDS             complement(547..1005)
                     /locus_tag="VHS06_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2988066.1"
                     /translation="MMQRLLDANGLPQYERILYVTDGSPAAQAAGRHAVFLAQRSQAQ
                     LIVLDIIPNSITRRISCLMRGALGEERRIAREAVEEIANLAAASGVGTVTAIESGHRG
                     DAIDRAASRFDVDLVVMSSPGGEDLQRIFGPPHAGSAPLWETRPICVIMC"
     gene            complement(1176..1883)
                     /locus_tag="VHS06_07855"
     CDS             complement(1176..1883)
                     /locus_tag="VHS06_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2988067.1"
                     /translation="MKILIADDEPDVVEVLEMTFKMHWPDAAIFTASNGEEALTTFFE
                     HQPGLVLLDIAMPELSGLEVCRRIRRVSNVPIIMLTVKDEEIDKVRGLEAGADDYVTK
                     PFGHLELIARARALLRRTGLTACNDDAGSFDNGYLSVDFSSCEVRVSGRRVLLTPTEF
                     QLLALLVRNPGQLLSHSLLLSRVWGPDCTEDRDYLKAYVKRLRQKLEPDPRNPTYILT
                     ERGFGYRFVPEGDSAVR"
     gene            complement(1864..>3691)
                     /locus_tag="VHS06_07860"
     CDS             complement(1864..>3691)
                     /locus_tag="VHS06_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988068.1"
                     /translation="HMCDSSRPNASRHEGGGSTCFMVKLLLAFRWIALAIAFLLNIWD
                     ASPAGFAHEPEAWIAAAAVYNAVVTLVLLRWRRLTPRQLLVADSLVFTGGVVMTGGWH
                     SSLYVLYFLIVLGAALQLRYVESLSYALAVGFVYAAACILLPNWEWELTAVEVVASRV
                     TVLLFAGVATIALSRQLEHEQNLRKAEEDVNSRLMVLNELMSLELGSRLDLDKTLEGI
                     ARLARRAISAEFSAVCLSAGPENSQFRVAFDGVPAQQQATMFREAQLDPIGEVVARTG
                     QPLLITDISRFSEGDHTLPGFFKCRTLVCAPIKLDDEVLGVLYNGVNSPERIQQSDME
                     LLVTMGRHTALAIVNAQMYDRERSNVVRLEKLEQMKSEFLSTVSHQLKTPITSIATSA
                     DLLQDSSGSLSEDQKRLVHNIARNAVRLDNLVTDLLQMARLKDGRVQLSVSPLRPGVL
                     VSDAVSAVRLLFEAKDQTVKVLMDPALPRVLADRRRVEHVLVNLLSNGCRYTQREGTV
                     SVEGREIDGSVEFAVRDDGPGMDRAVMERAFEPFFSATGPEGQGGTGLGLAIAKGLIE
                     LHGGAISIDSSIGTGTTVRFTLPVAQESKPTLEAEVDEDSDS"
BASE COUNT          753 a         1156 c         1095 g          687 t
ORIGIN      
        1 tcatatgtgc gacagtagcc ggcccaatgc tagccggcac gagggcggag gttcgacttg
       61 cttcatggtc aagctccttc tggcgtttcg gtggattgcg ctcgccatcg ccttccttct
      121 taatatctgg gatgcctctc ccggctgggg acgtcctgga tggagtcgtc ccgaattacc
      181 cttgcgttgg gcgcggccat cttcttcagc gctgccatgg ccgcctcggc cttggactcc
      241 tggacaacgc ccaagacggc gttcaggacc acgatggtca tgatcatccc tgcgtcggtc
      301 aactcgccga gggccgccga gacgacgctg gccacgatca gcaggatgat caggaagttg
      361 ttcacctggc cccagagaag ctgtagaaag cctggtctcg gtttctcttt tagctcgttg
      421 ggtccgtagc gctccagacg ccttcgagcc tcttcgctcg ttagtccggt gttggcgtcg
      481 gtccccagct ccttgagaac ctgcgccaac tcgcagccgt gggcagccgt gctcattcat
      541 tacctcctag cacatgatca cgcagatggg acgggtctcc cacaacggag cactcccggc
      601 gtgagggggg ccaaagatcc tttggaggtc ctcacctcct ggggagctca tgacaaccag
      661 gtcgacatcg aacctggagg ctgctcgatc gatggcgtca ccgcggtggc cagactcgat
      721 ggctgtgacg gttccgaccc cgctggccgc ggccagattc gcgatctctt cgaccgcctc
      781 cctggcaatc ctcctttctt cccccaaggc tcctcgcatc aggcaactga tgcgacgggt
      841 aatggagttg ggaatgatgt caagcacgat caattgagct tgagaacgct gggcgagaaa
      901 cacggcatgc ctgcccgcag cttgagcggc gggggaaccg tccgtcacgt agaggatccg
      961 ctcgtattgg ggcaacccat tcgcgtcgag tagccgctgc atcatcgcct tccctgaaaa
     1021 cgctgtggag atggttggcc gactggaggg ccgaccgccg tcgacaagag gaggggaaac
     1081 accttcgtgc ccaggaaaaa ggctaggatc ggacggtgaa aatcagggga acagacaggt
     1141 gaagggaagg gaaacgagaa gtgccagaag gggactcagc ggaccgctga gtccccttct
     1201 ggcacaaacc gatacccgaa gccccgctcc gtgaggatgt aggtgggatt acgggggtca
     1261 ggctccagct tctggcgaag ccgcttgacg tatgccttca agtagtcacg gtcctcggtg
     1321 caatcggggc cccaaacgcg ggacaagagc aaggaatggg acaagagctg cccggggttg
     1381 cgcaccaaca gagccagcag ctggaattcc gtgggggtca gaaggactcg acggccgctc
     1441 acccgcacct cgcaagagga gaaatctacg gagaggtagc cgttgtcgaa gcttcccgca
     1501 tcatcgttgc aggcagtaag gccggtgcga cggagcagtg ccctggccct ggcaatcaac
     1561 tccaggtggc cgaagggctt cgtgacgtag tcgtccgcgc ccgcttcaag cccccggacc
     1621 ttgtcgatct cctcgtcttt cacagtgagc atgatgatgg gcacgttgga gactcgacgg
     1681 atcctgcggc aaacctccaa cccgctcaac tccggcatgg cgatatccaa caacacgagg
     1741 ccgggctgat gctcgaagaa ggtcgtcagc gcctcctcac cgttggacgc ggtgaagata
     1801 gccgcgtccg gccagtgcat cttgaaggtc atctccagca cttcgacaac gtcgggctcg
     1861 tcgtcagcta tcagaatctt catctacctc agcctccagg gttggtttgc tctcttgggc
     1921 aacgggcagc gtgaaacgca ccgtggtgcc cgtcccgata ctgctgtcga tggagattgc
     1981 ccctccgtgc agctcaatca atcccttggc gatggcgagc cccaggccgg tgcctccctg
     2041 cccctccggg ccggtcgcgc tgaagaaggg ttcgaaggct cgctccatca ccgccctgtc
     2101 catcccgggg ccatcatctc taaccgcgaa ctccaccgaa ccatcaatct cgcgaccttc
     2161 cacactcaca gtgccctcac gctgggtgta gcgacaaccg ttggagagaa ggttgaccag
     2221 gacgtgctca acacgtctgc ggtctgccag cacccgcggc aacgcaggat ccatcagcac
     2281 ctttaccgtc tggtccttgg cctcgaacag cagccgcacg gcagagaccg cgtcgctcac
     2341 caggacgccc ggccgaaggg ggctgacgga cagttgaacc ctaccatcct tgagccgagc
     2401 catctgcagc aggtcggtca ccaggttgtc cagccgcaca gcgttcctgg cgatgttgtg
     2461 aaccagcctc ttctggtcct cgctcaagga accgctggag tcttgaagca ggtcggcgga
     2521 agtggcgatg gaggtaatgg gggtcttcag ctgatgggag actgtggata ggaactcact
     2581 cttcatctgc tccagctttt ccagcctcac cacgttactg cgctcccgat cgtacatctg
     2641 agcattgacg atggccagcg cggtatggcg gcccatggtg accagtagct ccatgtcgct
     2701 ctgctggatc ctctcgggcg agttcacacc gttgtacagc actcccaaca cctcatcgtc
     2761 cagcttgatg ggagcacaaa ccagcgtcct gcacttgaag aaaccgggta aggtgtgatc
     2821 cccctcggag aacctggaga tgtcggtgat cagcagcggc tggcccgtcc gcgccaccac
     2881 ctcgcctatc ggatccagtt gggcctcacg gaacatggtg gcctgctgtt gagcgggaac
     2941 accgtcgaag gccactcgaa actgggagtt ctcaggtcca gcggagaggc agacagcgct
     3001 gaactcggca gatatggctc gacgggccaa tcgggcgatg ccctccagcg tcttgtccag
     3061 gtcgagccgg gagcccaact ccaggctcat caactcgttc agcaccatca gcctggagtt
     3121 tacatcctcc tcggccttcc ggagattttg ctcgtgctcc aactgcctgc tcaaggctat
     3181 ggtagccaca ccggcgaaaa gcagtaccgt caccctgctg gccacgacct cgacggcagt
     3241 caactcccac tcccagttgg gcaacagaat gcaggccgct gcatagacga atcccaccgc
     3301 gagagcatag ctcagactct cgacgtagcg gagttgcagt gcggcgccca aaacgatcag
     3361 gaagtagagc acgtagaggc tgctatgcca cccaccggtc atcaccacgc cgccggtgaa
     3421 gaccaaactg tcggcaacca acagttggcg aggtgttagc cggcgccatc ggagcaggac
     3481 caaggtcaca acggcgttat agaccgcggc agcggctatc caggcctcgg gttcgtgggc
     3541 aaagccagcc ggagaggcat cccagatatt aagaaggaag gcgatggcga gcgcaatcca
     3601 ccgaaacgcc agaaggagct tgaccatgaa gcaagtcgaa cctccgccct cgtgccggct
     3661 agcattgggc cggctactgt cgcacatatg a
//
LOCUS       DATALS010000352         5564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_333280, whole genome shotgun sequence.
ACCESSION   DATALS010000352 DATALS010000000
VERSION     DATALS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333280"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VHS06_07865"
     CDS             <1..340
                     /locus_tag="VHS06_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite reductase, dissimilatory-type beta
                     subunit"
                     /protein_id="HEX2988069.1"
                     /translation="PAMPIADPENDGVAIFVGGKVSNARSAPMFSRLAIPYLPNTPPR
                     WPETVAAVKNIVEVYMRHAHRGERMGEWIERVGWERFFSLTGIPFTDKHIDDFRFAPT
                     TFRTSAAFKW"
     gene            410..610
                     /locus_tag="VHS06_07870"
     CDS             410..610
                     /locus_tag="VHS06_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988070.1"
                     /translation="MNEELRARVLAYLDKVDKAKNRDIATALGEPKHDVDKVVNELAK
                     EDRIEFLYLGTSYVALKGKYQG"
     gene            971..1486
                     /locus_tag="VHS06_07875"
     CDS             971..1486
                     /locus_tag="VHS06_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HEX2988071.1"
                     /translation="MSSKLLSSHRAAILPRWVQLILQSYPADTARLLESEPDQFLNPV
                     GSNISREAETLFGEIEGEMGEEKLSASLDSLMSIRAVQDFPPSKAVGIIFLLKQAVRE
                     GLKDEPHDARFYEELQEIESRVDRVALMAFDQYMGRREKIYELKLREASERADAILGR
                     MYFVPGAPDEM"
     gene            1740..2768
                     /gene="dsrM"
                     /locus_tag="VHS06_07880"
     CDS             1740..2768
                     /gene="dsrM"
                     /locus_tag="VHS06_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrM"
                     /protein_id="HEX2988072.1"
                     /translation="MKALYPLLVVLLLAALGFVGGMVDGLRPLLAVAVPYAALLLFAG
                     GFSYRVLMWARAPVPYHIPTTCGQGKSLPWIRASNLEAPHNTAGVVGRMALEVLFFRS
                     LFRNVSFTIERQRLLYWGEQLLWLGALVFHWSLLVIVLRHARFFTEPVPGFVNLLQGL
                     DGFFQVGEPVVYLTTVGFLLAGTFLLYRRWALPQVRYISLPGDYLPLFLILAIGLTGV
                     LMRHFFRTDIVAVKDLAMGLVTLRPVVPAGVSPLFYAHLFLVSGLFAYFPLSKLMHMG
                     GIFLSPTRNLANNSRMRRHVNPWDYPVPLHTLAEWEAEFKEKLQLAGIPLEDDGDEAE
                     VLAGTKDL"
     gene            2921..4549
                     /locus_tag="VHS06_07885"
     CDS             2921..4549
                     /locus_tag="VHS06_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2988073.1"
                     /translation="MAGPKAKPEELMRVDHRPPERGWEDPYIDFEGRRGSYCYSAKPK
                     VLDYLGMPNPRDWQPKDPDWKLPPNWKEIILEGMRERLGKYRSFQLFMDICVRCGACA
                     DKCHYFIGSGDPKNMPVLRAELMRSVYRRYFTTAGRLLGKHAGARDLTEDVLKEWFFY
                     FYQCTECRRCSVFCPYGIDTAEITMIGRELLNLVGVNINWIMEPAANCYRTGNHLGVQ
                     PHTFKDNIEFAADDLKDITGVKAETPINKKGAEVLLIVPSADYFGNPHYYTFLGYLAI
                     LHEAGLDYTFSAFASEGGNFGLFTSHEMMKRLNAKIYSEAKRLKVKWILGGECGHMWR
                     VIHQYMDTHNGPADFLEEPVSPITGTRFENARTTRMVHMAEFTADLIKNGKLKLDPSR
                     NDNWRVTYHDSCNPSRAMGLLEEPRFLLKAACNHFHEMPENTIRERTFCCGSGSGLGT
                     DENMEMRLRGGLPRANAVKHVQERHGVNMLACMCAIDKATLPTLLEYWVPGVEVCGIH
                     ELVGNALIMRGENERTTNLRNEPIEADKEVEADV"
     gene            4542..4928
                     /gene="dsrJ"
                     /locus_tag="VHS06_07890"
     CDS             4542..4928
                     /gene="dsrJ"
                     /locus_tag="VHS06_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrJ"
                     /protein_id="HEX2988074.1"
                     /translation="MYDAGKIFPGLGVFFVLLIFPFWFNGASGQGGVKPDIVKPTQER
                     QCIESADYMRANHMQLLIGWREEVVRNGPGQYVATDGKKYDMSLTGSCLKCHSNKTEF
                     CDRCHDYAGVQPNCWNCHTVPPEKKT"
     gene            4925..>5564
                     /locus_tag="VHS06_07895"
     CDS             4925..>5564
                     /locus_tag="VHS06_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2988075.1"
                     /translation="MRRADRRGFLKLAGLVVVGAGIKPTVDLLAGPELAEASRTGVAD
                     GRTGRRLAMAIDLPACLAAGDCTECIAACNAAHNIPHFDDPKEEIKWIWRDGYANAFP
                     DSANSFVATSLRNGQVTLLCNHCDNPPCVRVCPTQATWKREDGIVMMDEHRCIGCRYC
                     TVACPYCARSFNYRDPRQGLDMKTLTSDFPTRTRGVVEKCNFCVERVDKGQPP"
BASE COUNT         1089 a         1650 c         1804 g         1021 t
ORIGIN      
        1 cccggcgatg cctatcgccg accccgagaa cgacggtgtt gcgatcttcg tcggcggcaa
       61 ggtgtccaac gctcgcagcg ctccgatgtt ctccaggctc gcgatcccct acctgccgaa
      121 caccccgccg cgttggccgg agacggtggc agccgtaaag aatatcgtcg aggtgtatat
      181 gaggcacgcc cacaggggcg agcggatggg tgagtggatc gagcgggtcg gctgggagcg
      241 cttcttcagc ctgaccggca tccccttcac cgacaagcac atcgacgact tccggttcgc
      301 acctaccacc ttcaggactt ccgcggcgtt caagtggtag ctggccgcta tccgagattt
      361 ggggttggga gttgggggtt gggggacgga actaaggaga gttgttaggg tgaatgagga
      421 actgagggcc agggtgctgg cgtatctgga taaggtggac aaggcgaaga accgggacat
      481 agcgaccgcc ctgggcgagc ccaagcacga cgtcgacaag gtcgtgaacg agttggccaa
      541 ggaggacagg atcgagttcc tgtatcttgg tacctcctat gttgccctga aggggaagta
      601 ccagggataa tcgagtgctg agtgctgagt gcatcggcgc agtagtggtg atgaccgatg
      661 tcatgctgag cgaagcgaag catctcctcg tgaacggaga gatccttcgc tgtcgctcag
      721 gatgacactt ggcagggtga cagtggtcga agtagtgctc gaccacgcgg aattcgatgg
      781 gtaggagcga cacatatgtc gcccgagggc gacgcatgcg tcgcccctac aggtcgaaac
      841 cactgtggtc atacactgag cgcccgatct cggctcgggt gatgggtgtg ggttgggaca
      901 gcccaggtcg ctccaaccgc cgccatggcc cgtgcaccat ccatttcaca ggaagaggta
      961 tcacacgacg ttgtcatcta agctcttgag ctctcaccgt gccgcaatcc ttcctcgatg
     1021 ggttcagttg attctgcaga gctaccctgc cgataccgcc aggctcctcg agagcgagcc
     1081 ggaccagttc ctcaatcccg tggggagcaa tatctcgcgc gaggcggaga cgctattcgg
     1141 cgagatcgaa ggggagatgg gcgaggagaa gctctcagcc agcctggaca gcctcatgag
     1201 catccgcgcc gtccaggatt tccctccctc caaagcggta ggaatcatct ttcttctcaa
     1261 gcaggctgtt cgggaggggc ttaaggacga gccacacgat gcccgattct acgaggaatt
     1321 gcaggagatc gagtctcgcg tggacagggt ggcgctgatg gccttcgatc agtacatggg
     1381 gcggcgcgag aagatttacg agctcaagct tcgagaggca agcgaaaggg ccgatgccat
     1441 tctggggcgg atgtacttcg tgcccggcgc acccgacgag atgtagaccg cgtgcaggaa
     1501 agggaccttg ccacgactgg cgtatgtagg attggggata gctggcaact atcatagggc
     1561 ggggtcttgt gccccgcctg gagcgtagcc gaaacacaat ttggtggtcg tggcgaggct
     1621 gtgggcggcg gggcacaaga ccccgcccta cgagcgtgac aacacgacag cacttcttga
     1681 tacgagcctt gcagacccgg caaacgcttc ggtcgtgaca tctggggagg ctgagctaga
     1741 tgaaggcgct gtatcccctg ctggtggtcc tactcctggc ggccctggga ttcgtaggag
     1801 gcatggtgga tgggctgcgg cccctcctcg ccgttgccgt gccgtacgcg gcccttctgc
     1861 tcttcgccgg cggcttcagc taccgcgtac tgatgtgggc ccgagcgccc gtgccctacc
     1921 acatccccac cacctgcgga cagggaaagt cgctgccctg gatccgtgca agcaacctgg
     1981 aggcacccca caacacggcc ggcgtggtgg gcagaatggc gctggaagtg ctcttcttcc
     2041 ggtcgctgtt ccgcaacgtg tccttcacca tcgagaggca gcggctgctc tactggggcg
     2101 agcaacttct ctggctgggc gccctggtct ttcactggtc actgctggtc atcgtgctgc
     2161 gccacgcccg cttcttcacg gagccggtgc ccggcttcgt caaccttctg caggggctgg
     2221 acggcttctt ccaggtgggg gagccggtgg tgtacctgac cacggtgggg ttcctgctgg
     2281 ctggcacgtt cctactctat cgcaggtggg cgcttcctca ggtccgctac atctctctac
     2341 cgggcgacta cctgcccctc ttcctgatcc tggccatcgg cctcaccggc gtgctgatgc
     2401 gccatttctt ccgaaccgac atcgtggctg tgaaggatct ggcgatgggc ttggttaccc
     2461 ttcggccggt cgttcccgcg ggcgtctctc cgctcttcta cgcccacctc ttcctggtta
     2521 gcggactctt cgcctacttc ccgttgagca agctgatgca catggggggc atattcctga
     2581 gcccgaccag gaacctcgcc aataacagcc gaatgcggcg ccatgtgaac ccgtgggact
     2641 atcccgtacc cctccacacc ctcgcagagt gggaggccga gttcaaggag aagctccaat
     2701 tggccggcat ccccctggaa gacgatgggg atgaggcgga ggtgttggcc ggaactaagg
     2761 atttgtaggg gcgacgcgtg cgtcgcccgg gcgacatgta tgtcgcccct acgttagggc
     2821 gcgggcgtct cgcccgcgat tacacggctg aagcgacggc cggcgcatac gatcgcggtg
     2881 ggcgtggtcg tccaacaatc tgccttcgta gggggtagag atggctggac ccaaggcgaa
     2941 accggaagag ttgatgaggg tggatcaccg gccaccggag cgcggctggg aggatccgta
     3001 catcgacttc gagggccgca ggggttccta ctgctatagc gccaagccga aagtgctgga
     3061 ttacctgggc atgcccaacc ccagggattg gcagccgaag gacccggatt ggaagctccc
     3121 ccccaactgg aaggagatca tcctggaggg gatgcgcgag cggttgggca agtatcgctc
     3181 cttccagctg ttcatggata tctgcgtgcg gtgcggcgcc tgcgccgaca agtgccacta
     3241 cttcatcgga tccggcgacc cgaagaacat gccggtgctg cgggcggagt tgatgcgctc
     3301 ggtctaccgc cgctacttca ccacggccgg aaggctgctg ggcaagcatg caggtgcccg
     3361 agatctcact gaggacgtgc tgaaggagtg gttcttctac ttctatcagt gcaccgagtg
     3421 ccggcgatgc tcggtcttct gcccctacgg gatcgacacc gccgagatca cgatgatcgg
     3481 ccgggagttg ctcaacctgg tgggggtgaa catcaactgg atcatggagc ccgccgccaa
     3541 ctgctatcgc accggcaacc acctgggagt gcagccccac accttcaagg acaacatcga
     3601 gttcgccgcc gatgacctca aggacatcac cggcgtaaag gcggagacgc cgatcaacaa
     3661 gaagggggcg gaggtgctgc tgatcgtgcc ctccgccgat tacttcggca acccccacta
     3721 ctacaccttc ctgggctacc tggcgatcct ccacgaggcg gggttggact acaccttcag
     3781 cgccttcgcc tcggagggcg gcaacttcgg gttgttcacc tcccacgaga tgatgaagcg
     3841 actgaacgca aagatctact ccgaggcgaa gcggctgaag gtcaagtgga tcctgggcgg
     3901 cgagtgcggc cacatgtggc gggtgatcca ccagtacatg gacacccaca atggccccgc
     3961 ggacttcctg gaggagccgg tttcgcccat caccggcacc cgcttcgaga atgcccgcac
     4021 caccaggatg gtgcacatgg cggagttcac cgccgacctg atcaagaacg gcaaactgaa
     4081 gctggacccc agccgcaacg acaactggcg ggtaacctac cacgactcct gcaacccatc
     4141 acgtgccatg gggttgttgg aggagccgcg cttcctgctc aaggctgcct gcaatcattt
     4201 ccatgagatg ccggagaaca ccatccgtga gcgcaccttc tgctgcggaa gcggctccgg
     4261 actgggcacc gacgagaaca tggagatgcg gctgagaggg gggctcccca gggccaacgc
     4321 tgtgaagcac gtccaggaga ggcatggggt gaacatgctg gcctgcatgt gcgccatcga
     4381 caaggccacg ctgccgacgt tgctggagta ctgggttccc ggagtggagg tgtgcggcat
     4441 tcacgagttg gtgggcaacg cgctgatcat gaggggggaa aacgagcgga ctaccaacct
     4501 gcgcaacgag ccgatagagg cggacaagga ggtcgaggcc gatgtatgac gccggcaaga
     4561 tcttcccggg cctcggggtg ttcttcgtgc tgctgatctt tcccttctgg ttcaacggtg
     4621 cgtctggcca gggtggcgtg aagccggaca tcgtcaagcc tactcaggag aggcagtgca
     4681 tcgaatcggc ggattacatg cgcgcgaacc acatgcagct gttgatcggc tggcgggaag
     4741 aggtggtgcg gaacggcccg gggcaatatg tcgctaccga cggcaagaag tacgacatga
     4801 gtctcaccgg cagctgtctg aagtgccaca gcaacaagac ggagttctgc gaccgctgcc
     4861 acgactacgc gggggtgcag ccgaactgtt ggaactgtca caccgttccc ccggagaaga
     4921 agacatgaga cgtgccgaca ggaggggctt cctgaagctg gcgggactgg tcgtcgtcgg
     4981 ggctgggatc aaaccgactg tggatctcct ggccggacca gagttggcgg aagcctcgcg
     5041 cacaggggtg gcagacggac ggactgggag aaggctcgcc atggcgatcg acctcccagc
     5101 ctgcttagcg gccggtgatt gcaccgaatg catcgcggcg tgcaacgccg cccacaacat
     5161 cccccacttc gacgatccga aagaggagat caagtggata tggcgggatg gctacgccaa
     5221 cgcattccca gactcggcca actccttcgt cgcaaccagt ctgaggaacg ggcaggtaac
     5281 ccttctgtgc aaccactgcg ataacccgcc ctgcgttcga gtgtgtccga cccaggcaac
     5341 ctggaagcgg gaggacggca tcgtgatgat ggacgagcac cgctgcatcg gctgccgcta
     5401 ctgcactgtg gcctgcccct actgcgcccg cagcttcaac tacagggacc cgcgacaggg
     5461 tctggatatg aagacgctca cctccgattt ccccacacga acgaggggag tggtggagaa
     5521 gtgcaacttc tgcgtggagc gtgtcgacaa gggacagcca ccgg
//
LOCUS       DATALS010000353         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_38816,
            whole genome shotgun sequence.
ACCESSION   DATALS010000353 DATALS010000000
VERSION     DATALS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38816"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VHS06_07900"
     CDS             <1..382
                     /locus_tag="VHS06_07900"
                     /inference="COORDINATES: protein motif:HMM:NF025109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HEX2988076.1"
                     /translation="RTMLLCTHNLAEAEELCDRAIILRSGVVLVDDAVESLRGRFTRP
                     LVLEAADDPERVAEMVRLEGHSASVDSRHVRVDVSDYRREVPKLLAKLLAAGIQVYGA
                     QVQEPSLEEVFLRLMGEVRGGDDV"
     gene            375..1118
                     /locus_tag="VHS06_07905"
     CDS             375..1118
                     /locus_tag="VHS06_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2988077.1"
                     /translation="MFSNEFRVLFGKEWKQAVRNRGAVLTAVLFPVLFLLVVPGAQAF
                     AASQGIATSNLTATNLPPGLTDVSPKAIFTQMMFPMFLVIGGLVVPSVAASYTVVSER
                     EKRTIDLLTALPVSLGQVIGAKLAAISALGILITLPLALIDCALLLVLGLADPLLISG
                     YLFELFAALLFSVASAMATALFAKDFRSSNNISGLFMGPMIVLTLGFGLLLPWGLYRL
                     FLHGALLVALGIGILFVALKTVSVERFAL"
     gene            complement(1195..2100)
                     /locus_tag="VHS06_07910"
     CDS             complement(1195..2100)
                     /locus_tag="VHS06_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX2988078.1"
                     /translation="MILPRLRFAIFIALLAVVALHSPAARAGEPDGEATTQNGDAPAD
                     ATFQSTSVWPPEMELINSYRAAAGVRPAEINPSLDSSASSHVRYYDANRGDPSLAGMG
                     LHSETPGRPGFTGATMGNRARAAGYSGGTVTENAGYGSFAGAVDWYMNSVNHRLPLIH
                     PSALDMGYAESPESRFSIVSVGLRHESVQSPSLYPGPDAQGVPTSWDGSETPNPAPGL
                     ARPLGYPITVAFGVSQRVAWSSFSLSGPEGEPVEFVVSRTDWMRAAAIIPTRPLQPGA
                     TYTVQVEATVDGKPFSKEWSFTTAG"
     gene            complement(2163..>2652)
                     /locus_tag="VHS06_07915"
     CDS             complement(2163..>2652)
                     /locus_tag="VHS06_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX2988079.1"
                     /translation="YPITVAFGVSQRVAWSSFSLSGPEGEPVEFVVSRTDWMRAAAGL
                     APLQWNNELAIAASDYAFYMASHSYFAHNTPEGTTPVSRSSAAGYPSFPWGTYVGENL
                     AKGFDSPDTVMQGWMNSEGHRRNVLLPDYIEIGVAVAAAPDGTLYWAQEFGNRPAIYA
                     EE"
BASE COUNT          406 a          831 c          899 g          516 t
ORIGIN      
        1 ccgcacgatg ctgctgtgca cccacaacct cgccgaggcg gaagagttgt gcgacagggc
       61 gatcatcctt aggagcggag tggtgctggt cgacgacgcc gtggagagcc tacgcggccg
      121 cttcaccagg cccctcgtgc tggaagcggc ggatgatccc gagcgggtgg cggagatggt
      181 ccgcctcgaa ggccacagtg ctagcgtcga cagccggcac gtaagggtgg atgtctccga
      241 ttacaggcgg gaggtgccga agttgctcgc gaagcttctc gccgccggta tccaggtgta
      301 cggggcgcag gtgcaggagc cttccctgga agaggtgttc ctcagactta tgggagaggt
      361 tcgaggaggc gacgatgttt agcaacgagt tcagggtcct gttcggcaag gagtggaagc
      421 aggcggtgag gaaccgcggc gccgtgctga cggcggtgct tttccctgtg ctgttcctgc
      481 tggtggttcc tggggcccag gcgttcgccg cgagccaggg aatcgcaacc tctaacctga
      541 ctgcgaccaa ccttccgccc ggcctcacgg atgtctcgcc gaaggcgatc ttcactcaaa
      601 tgatgttccc gatgttcctg gtaatcgggg ggctggtggt tccgtccgtc gccgctagct
      661 ataccgtggt cagcgagagg gagaagagga ccatcgacct gctaacggcg ctgccggtat
      721 cgctcggtca ggtcatcggc gccaagctgg cggccatctc ggccctcggc attctcatca
      781 cgctgccctt ggcgctgatc gactgcgcgc tgctgctggt cctcgggctg gcggacccgc
      841 tgctcataag cgggtatctc ttcgaactgt tcgcggcgtt gctcttctca gtcgcatcgg
      901 cgatggccac agcgctgttc gcgaaggact tccggtcgtc gaacaatatc agcgggctgt
      961 tcatggggcc gatgatagtc ctcaccctcg gcttcggatt gctcctgccc tgggggctct
     1021 accggctctt cctccacggc gcgctgctcg tggcgctggg gattggaatc ctgttcgtgg
     1081 cgctgaagac ggtaagcgtg gagcggttcg ctttgtaggg ggacgcggag acacggagaa
     1141 gacgatccgt agggcggggg cttgtcgccc gccgccttcg tcccctattc cctgctatcc
     1201 cgccgtggtg aagctccact ccttcgagaa cggcttgccg tcgacggttg cctctacctg
     1261 cacggtgtag gtggcgcctg gctggagcgg gcgcgtgggg atgatcgcgg cggcgcgcat
     1321 ccagtcggtc ctgcttacga caaactccac gggctcccct tccgggccgc tgagcgagaa
     1381 ggagctccac gcgaccctct ggctcactcc gaaagccacg gtgatcgggt acccgagcgg
     1441 cctggcgagc ccgggcgcgg ggttaggcgt ctcgcttccg tcccacgagg ttgggacgcc
     1501 ctgagcgtcg ggcccggggt agagcgaggg ggactggaca ctttcgtgcc tcaggccgac
     1561 ggagacgatg ctgaagcggc tctccggcga ctccgcgtag cccatatcca gggcacttgg
     1621 gtggatcagc ggcagcctgt ggttgacgct gttcatgtac cagtcgacgg cccccgcgaa
     1681 gctgccgtag cccgcgttct ccgtgaccgt gcctccgctg tatccggccg ctcttgccct
     1741 gttgcccatc gtcgctccgg tgaaccccgg cctgcccggc gtttcgctgt ggaggcccat
     1801 tcccgcgagg gaggggtcgc ctctgttggc atcgtagtac cggacgtggc tggatgcgga
     1861 actgtcgagc gaggggttga tctctgccgg gcgaaccccg gccgccgccc tgtagctgtt
     1921 gatcagttcc atctccggcg gccagacgga cgtgctctgg aaggtagcat ccgccggggc
     1981 gtctccattc tgggtcgttg cctcgccgtc tggttccccg gccctcgccg ccggcgaatg
     2041 cagcgctacc acggcaagca gcgcgatgaa gatggcaaaa cgtagccgtg gcaggatcat
     2101 caaccacctc gttggggggc aaattgaaag ggtcaccacc gggtgaccct ggagaactct
     2161 gcctattctt ctgcgtagat ggcaggtctg ttgccgaact cctgggccca gtagagggtt
     2221 ccgtccggcg ccgcggccac ggccacgccg atctcgatgt agtcgggaag aagcacgttt
     2281 cgcctgtggc cctcgctgtt catccacccc tgcatcacgg tgtcggggga gtcgaacccc
     2341 ttcgccaggt tctcgcccac gtaggtgccc cacgggaagg agggataccc tgccgctgag
     2401 ctcctgctga caggggtcgt cccctcgggc gtgttgtggg caaagtaact gtgagatgcc
     2461 atgtagaagg cgtagtcgct ggcggcgatg gcgagttcgt tgttccactg gagaggagcg
     2521 agacccgcgg cggcgcgcat ccagtcggtc ctgcttacga caaactccac gggctcccct
     2581 tccgggccgc tgagcgagaa ggagctccac gcgaccctct ggctcactcc gaaagccacg
     2641 gtgatcgggt ac
//
LOCUS       DATALS010000354        11198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_172852, whole genome shotgun sequence.
ACCESSION   DATALS010000354 DATALS010000000
VERSION     DATALS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172852"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..2212
                     /gene="mrdA"
                     /locus_tag="VHS06_07920"
     CDS             176..2212
                     /gene="mrdA"
                     /locus_tag="VHS06_07920"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX2988080.1"
                     /translation="MCTKWRLLSIVGLLLLLPLLSSCSNKPSAYAVLEPYLKAWEEAR
                     YSDMYDLLSTASQASITRERFVQRYAAITEGSTVQSVKTAFTRDDKLAKEEKAADLPF
                     IVTFSTVRLGEFKEDNILPTVYEDEKWKVNWSPSLIFKDLSGDNRILFEPQDPQRGSI
                     LDRKGRPLATSGKLTSVGVVPGQIQDEGKLLSTLEQQLQIPAAQIKRAYQSAQPDWFV
                     PLTDLSAAEAATVRPKLESLPGVMFQEKAGRVYPNGTTAAQVVGYMSKVTADDLKARA
                     AEGLSEDDMVGRAGIEAFAEKTLAGERGGKLSVTTSDGQPVKVIAQKPAKNGQDVQLT
                     LDLDAQKLAEESLGSQAGSVVMLNVQDNSVLALASYPRFDPNLFITGISGADWKKLND
                     DPLHPFQNRPVASSYPIGSVFKPVTMAAGLEKGGFTADSPFDCNGHWDGLGSGKVLGD
                     WLPQGHGHLTLFQGLFQSCDIVFYELGKKLDSIDPKILPQYARGWGYGQPTGLVGLEE
                     ASGQVPDPDWKQANQKDAWYLGDSVNLAIGQGFFLATPLQVANAYSALARGGALMTPV
                     LVSKQGDLGLQAQPKGNISATPATQNLIRQAMKAVVSDPKGTATYAFQGEKFSIAAKT
                     GSAEAQGPDSHAWFASFAPAEQPQVAMVVMVESKGLGSEVAAPVARKILDGYLK"
     gene            2359..3288
                     /gene="hpt"
                     /locus_tag="VHS06_07925"
     CDS             2359..3288
                     /gene="hpt"
                     /locus_tag="VHS06_07925"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX2988081.1"
                     /translation="MSIHLLKPVASTRYPKGSVHHAGIKFVHPSEAEFARILDFYRIK
                     WIYEPKSFPLRWDGDRVTEMFTPDFYLPGLDTYVELTTMKQSLVTKKNRKLRELRELY
                     PEVNIRLLYRRDYQSLLAKYGYRAITPTSLEDIDHVLLSADEIQKRVDELAEEINRDY
                     AGQELRLVGILKGVAPFMTDLMRKLTMPVSVDYMFISKYIRDGSESEFVRILKDLDNP
                     IEGTHLLLVEDIVDTGFTLRYILNYLRARKPASLKVCTLLDKRARRLVDQPLDYVAFE
                     IPDEYVVGYGLDHRELYRNLPFVCVLKPEAYRL"
     gene            3399..3863
                     /locus_tag="VHS06_07930"
     CDS             3399..3863
                     /locus_tag="VHS06_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX2988082.1"
                     /translation="MGAAGPVIPTIAGCGFRWWFLLVAALPLLLLACRQEAATDRDYR
                     VEISVSPERPSVGPAEIAVRLFDGTGTAVKGATVEVEGNMNHAGMVPVRAVAEESAAG
                     GYLTRDFRFTMGGDWIITVRAGLPGGRVEKSFDLKGVSSGQMQMEQPAVNKP"
     gene            3860..5155
                     /locus_tag="VHS06_07935"
     CDS             3860..5155
                     /locus_tag="VHS06_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FesM"
                     /protein_id="HEX2988083.1"
                     /translation="MKREAVAVETPARLDLLKTPLLGAFLRWRHSRSALQLVSLLVAA
                     LVVYDGLTGPQLAPKNLAGVVPWVQWRGLIVLALLLVGNLFCMACPFMLPRQLAKKLL
                     PARLSWPGRLPAKLFAVPLLILFFWSYEAFSLWSSPWLSAWLALAYFACAFLVDGLFK
                     GAAFCKHLCPIGQFHFVNSVASPLEVGVRSPGRCLACTSKACIKGRRDDAGRALRGCE
                     LWLFQQRKTGNLDCTFCLDCAKACTEDNVGILGRKPVSELWRDRSRAPATDFARRPDL
                     AAMAVVLVAAAFMNAFGMVAPVYGVERWIAGSLGTESRLLVLAVLFGFGLVVVPALLV
                     SIAGWIGRYLAGSRRPVIQVASYYSFALVPLGFGMWLSHYSFHFLTGALTVLPVVQSL
                     LSDLGLPRIGSPQWGLGPVVPFSWLMPVELLFLEGGLLA"
     gene            5174..5317
                     /locus_tag="VHS06_07940"
     CDS             5174..5317
                     /locus_tag="VHS06_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988084.1"
                     /translation="MAAREEGDSGKTLTAFLPWALLCLAPFAAGVWLMQQPMEMRGTF
                     VGG"
     gene            5322..5951
                     /locus_tag="VHS06_07945"
     CDS             5322..5951
                     /locus_tag="VHS06_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX2988085.1"
                     /translation="MASTLLKRWAILRAVLSMLAVGVLLSSCTPQDARLAGIDMKGEP
                     ATDFRLTGHAGDTVQLSSLRGRVVVLTFLYTSCPDTCPLIAGKFGVVHDRLGQLAQGS
                     SFLAVTVDPERDTPYRLHQYLEAQQLQGKMDFLTGSRPDLEKIWTAYHVGVQRPPAHA
                     EVGGSGQGYEVAHTEAVYVIDKQGKLRRLFKADFEPAALAKDVELLSRE"
     gene            5997..6914
                     /locus_tag="VHS06_07950"
     CDS             5997..6914
                     /locus_tag="VHS06_07950"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX2988086.1"
                     /translation="MSDESMALLTSWNLEPELVVGLMVAIGLYFVGWMRLSRRTSEGG
                     PLIRWWAWCYCSGMAAIAIALLSPVETFSGLFFFVHMIQHLLLMLVAAPLLWLGAPIL
                     PLMWALPRRVRVGLGRMFACGRPLHALLNFLTNPLVAQALFLATLAGWHVPPYYDIAQ
                     GRSLVHEFEHTMFLGTSLLFWWPVVHPWGGKRRLSYEAGIAYFIPPILVSNLIGALLT
                     FAGRPLYTNYLHVPRLWGISVVLDQQLGGLIMWVPGMIPYAIAIFLLVSQLMKQGELR
                     EKEVEIAAAIQRKQLGTEPDEGAEEVDAL"
     gene            6904..7428
                     /locus_tag="VHS06_07955"
     CDS             6904..7428
                     /locus_tag="VHS06_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988087.1"
                     /translation="MPFRVTSLYVIVVVAAMAVGTTPAMANGGTIQLSNRPAGPYAVT
                     VYTSPTPIRAGTSDVSVMVQRAGSDELVNDARVVVQAQPIGRDGGLSSYQATHEQATN
                     KLFYAANVALPSEGRWRIGVQVEAAAGLGEVDFETDVAPAGLLDSPLLLVQLVALPLI
                     LAVLWLGSRKLSKG"
     gene            complement(7471..9018)
                     /locus_tag="VHS06_07960"
     CDS             complement(7471..9018)
                     /locus_tag="VHS06_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2988088.1"
                     /translation="MLRDRLGQLEAVQTYTERVPKVTDRLWERVPGVVSWLVALSPIW
                     VTLLSPILGLILVVAASVYFSARASWYGPKALINRHRVLKSQQDDWLARLEALEQDIP
                     DWRNCRVTLMIRAYHEKNLDMLRATLDSIYASNWPKEQGKMVNVEVLYATEEDDPYTP
                     PLVDKLAEEYADRVAIRQIKHPDEPNTLPGPSTAMNYTGRVLYREALRKGFPPNKWIV
                     ADFDADTLFDPQYIPCLVYHYVTDPKRDVRAYQPVVLFTTDYWKAPLHSRLSAIGTSV
                     LTLGWIKKPEIAFTGAAASLATLHSVGFWPTNSHSQDSGVELRLRMQYGRNFKVRGLP
                     IPLWVYPVMAMGAQSTLKERLRAYWRSYQALFRQSARWREGPLDEFAEATSRGKPWLA
                     FKRLWNGLERDTLTLMPSYGLIVASAMVDPSELGATTDMLKSLFAIGLTVVSLLGLLV
                     FWRVLGTDELVLGKRKPGQRIKELFLFWLVFSIYVPIFTSFAGLKTSTSYTLGKRPRG
                     HFMPTPK"
     gene            9456..11060
                     /locus_tag="VHS06_07965"
     CDS             9456..11060
                     /locus_tag="VHS06_07965"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX2988089.1"
                     /translation="MAFPAVIVGPLLRLGLLALLLNPMTALPAAHVSPAPLDVKVGSV
                     ADSSGQPTTKKLLLVDGKPFAPVGVTYHFTYHRDSWDDDLQAMHDMGLNSVRIDFSWR
                     DIEPILPGHYRFGVLDEFLDRASQHGLYVVPVFSHTTRDFNTPLWFWPDYDSWSSVDQ
                     NGQRPLDDLPSISYPEYRRRLSSYMAATVQHIKQHPAVLAYQLLNEPRYDLKRLSDYN
                     PYTVAAFRVWLREQYSTIDQLNDAWGTGYKSFEETQPARVADDDPPKDGPLLKQWSDW
                     RQFSYDNLADFIGELATTVKSADPGHPVIVAEMAWWWWGEQPYSGVSPLHIYKDADIV
                     GYDLYPDSIEDASYFLLTSDMLSRYWQKPVWVMEMNSKDGDPTGEETQKFVSSAMDGG
                     ATGVFYFEWQDDWRDGGNYGVLDSRGRRRTQYGALTSAVRWLRQKEESLVMAPTPKPD
                     VYLVWPSGEVGQVTGAESPAWDIYATARRIVEGGLRIGLVAEELMHAVDPSKLLTLRD
                     GRLENGKAPTPGPTPQSPSRGFLISD"
     gene            11110..>11198
                     /locus_tag="VHS06_07970"
     CDS             11110..>11198
                     /locus_tag="VHS06_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX2988090.1"
                     /translation="MQIAIIGLPMSGKTTVFNALTGARAKTGA"
BASE COUNT         2056 a         3282 c         3543 g         2317 t
ORIGIN      
        1 agcacccagc acccagcacc cagcacccag cacccagcac ccagcaccca gcacccagca
       61 cccagcaccc agcacccagc actcagcact cagcactcag cactcagcac tcagcactca
      121 gcttgtccca caagtgtata atgagcatca gctatactgt ggagatacta gctatttgtg
      181 cacgaaatgg cgtctcctat ccatagttgg cctcctgttg ttgctgccct tgctgtcctc
      241 ttgctcaaac aagccgtccg cctacgccgt gctcgagccc tacctcaagg cgtgggaaga
      301 ggcccgttac agcgatatgt acgacctgct ctccacagcc tcccaggcga gcatcaccag
      361 ggagcgcttc gttcagcgat atgctgccat caccgagggg agcacggttc agtcggtgaa
      421 gactgccttc acgagagacg acaaactggc taaggaggag aaggcagccg acctaccctt
      481 catcgtcact ttctccaccg tgcggctggg ggagttcaaa gaggacaaca tcctccctac
      541 cgtgtacgag gacgagaagt ggaaagtgaa ctggagccct tctctgatct tcaaggacct
      601 cagcggcgat aaccggatcc tcttcgagcc gcaggacccg cagcgcggct cgatcctcga
      661 taggaagggg cgtcctctcg ccaccagtgg caagcttacc tcggtgggtg tagtgcccgg
      721 ccagatccag gatgagggca agctactctc caccctggag cagcagcttc agatccccgc
      781 ggcgcagatc aagcgggcct accagagcgc ccagcccgat tggttcgttc cgttgaccga
      841 tctctccgcg gccgaggcag cgaccgtgcg accgaagctg gagtcgttgc cgggcgtcat
      901 gttccaggag aaggcgggaa gggtatatcc caacgggaca actgctgccc aggtggtcgg
      961 ttacatgagc aaggtgacgg ccgacgattt gaaggccagg gctgcggagg ggctttccga
     1021 ggacgacatg gtgggccgag ccggcataga ggctttcgcc gagaagaccc tggcagggga
     1081 gcgtggcggc aagctgagcg tcacgacctc cgacggccaa cccgttaaag tgattgccca
     1141 gaagccggcg aagaacggac aggacgtgca acttacgctg gacctggatg cgcagaagct
     1201 ggctgaggag tcgttggggt cccaggccgg cagcgtggtc atgctgaacg tacaggacaa
     1261 tagcgtcctt gcgctggcga gctaccctcg attcgacccc aaccttttta ttaccggcat
     1321 ctccggcgcc gattggaaga agctgaacga cgatccgctg caccccttcc agaaccggcc
     1381 ggttgccagt tcttatccca tcggatcggt gttcaagccc gtcactatgg ccgcgggtct
     1441 ggagaagggc ggcttcaccg cggacagccc cttcgactgt aacggccatt gggatgggtt
     1501 gggcagcggg aaggtgttgg gggactggct tccccaggga catggccacc tcaccctctt
     1561 ccaggggctg ttccagtcgt gcgacatcgt tttctacgag ttgggaaaga agctggactc
     1621 catcgatccg aagatccttc ctcagtatgc tcggggctgg ggatatggtc agcctaccgg
     1681 gttggtcggg ctggaagagg catctggtca ggtgcctgat cccgactgga agcaggcaaa
     1741 ccagaaggac gcctggtacc tgggcgacag cgtcaacctg gccatcgggc agggcttctt
     1801 cctggctact ccccttcagg ttgccaatgc gtactcggct ctcgcgcgcg gtggcgctct
     1861 gatgaccccg gtgttggtat cgaagcaggg cgacttgggc ctccaggctc agccgaaggg
     1921 caacatctct gccacgcctg ccacgcagaa cctcattcgc caggccatga aggctgtggt
     1981 ctccgacccg aaggggacgg ccacctacgc ctttcagggg gagaagttct cgattgccgc
     2041 gaagaccggc tctgctgagg cacaaggtcc cgactctcac gcctggttcg cctccttcgc
     2101 accggcggag cagcctcagg ttgccatggt ggtcatggta gaatccaagg gattgggctc
     2161 cgaggtcgcg gcgccggtag ccagaaagat cctagatggg tacctcaagt gacggagagc
     2221 gaaagaaagc cgggggagga agaccccttt aggggcggtt caagcacgga gcagcaacct
     2281 ggcggcggga attccatctc tccccaacca tcctcaagag gcaagacggt ccgaagcggc
     2341 ggagaacggc ggacgcgagt gtccatccac ctgctcaaac cggtggccag cacccgctac
     2401 ccgaaaggga gtgtgcatca cgccggcatc aagttcgtcc accccagcga ggcggagttt
     2461 gcccgcatcc tggatttcta caggataaag tggatctacg agccgaagtc gttccccctg
     2521 cggtgggacg gcgaccgtgt gacggagatg tttacccccg acttctacct tccgggcctg
     2581 gacacctacg tcgagcttac cacgatgaag cagagcctgg tcaccaagaa gaaccggaag
     2641 ctgagagagc tccgcgagct ctatccggag gtgaacattc gtctccttta ccggcgggat
     2701 taccagagcc tgctggccaa gtacggttac cgggccatca cgcccacctc cttggaagac
     2761 atcgaccacg tgctgttgag tgcggatgag atccagaaga gggtggacga gctggccgag
     2821 gagataaacc gcgactacgc cggccaggag ttgcggctgg tggggatact gaagggtgtc
     2881 gcccccttca tgactgacct gatgcgcaag ctgacgatgc cggtgtcagt cgactacatg
     2941 ttcatcagca agtacatccg ggatggatcg gagtcggagt tcgtccggat attgaaggat
     3001 cttgacaacc cgatcgaggg gacgcacctg ctgttggttg aggatatcgt cgataccggt
     3061 ttcactcttc gctacatact caactacctt cgtgctcgga aacctgccag tctgaaagtg
     3121 tgcacgttgc tggacaagcg ggcaaggcgg ctggtagatc agccgctgga ttatgtggcc
     3181 ttcgagatcc cggatgagta cgtggtgggc tatggcctcg accaccgcga gttgtaccgt
     3241 aatctgcctt ttgtctgcgt gctgaagccg gaagcgtatc gcctctaggt ggttcgagct
     3301 cctgactgcc tctgctctcc gaagcccgcg cgactggcgt tcttcttgca ccttctgcaa
     3361 cccaagaagt aagtggtggc acgaaaggag cggaggcaat gggcgcagca ggtccggtga
     3421 ttccgacgat tgccgggtgc ggtttcaggt ggtggttttt gttggtggcg gctctgccac
     3481 tcctgctgct tgcctgccgt caggaggccg cgacggatcg tgactaccgg gtggagattt
     3541 cggtgtcgcc cgagcgcccc tcggtggggc cggccgagat cgcagtgcgg ctttttgatg
     3601 gaactggcac cgccgtaaag ggggcaacgg tggaggtgga gggcaacatg aaccatgccg
     3661 gcatggtgcc ggtacgagcc gtggcggagg agtctgccgc cggcggctat ctgacccgcg
     3721 acttccgatt cactatgggg ggtgactgga taatcaccgt acgcgccggc cttcccggtg
     3781 gacgcgtgga gaagagcttc gatctcaagg gtgtgagctc agggcagatg cagatggagc
     3841 agcctgccgt gaataagccg tgaagcgcga ggcggttgcc gtggaaacgc cggctcggct
     3901 cgatctgctg aaaacacccc tgttgggggc cttccttcgg tggcgccatt cgcgcagcgc
     3961 gcttcagttg gtgtcgctgc tggttgcggc gctggtcgtg tacgatggcc ttaccgggcc
     4021 gcagttggca ccgaagaatc tggccggggt ggtcccctgg gtgcagtggc gtgggctgat
     4081 cgtattggcg ctgctgctgg ttggaaacct gttctgtatg gcctgccctt tcatgttgcc
     4141 caggcagttg gccaagaagc tgctccccgc tagactctct tggcccggcc gcctgcccgc
     4201 caagctgttt gccgtgccgc tgctgatcct cttcttctgg tcctacgagg cattttcgct
     4261 ctggtccagc ccctggctga gcgcatggtt ggccctggct tacttcgcct gcgccttcct
     4321 ggtggatggg ttgttcaagg gcgccgcctt ctgcaagcat ctctgtccca tcgggcagtt
     4381 tcacttcgtc aattcggtcg cctctcctct cgaagtaggt gtgcgcagtc ccggtcgatg
     4441 cctagcttgc acgagcaagg catgcatcaa gggccgccgc gacgacgcgg ggcgcgcgct
     4501 gcggggctgt gagttgtggc tgttccagca gcgcaagacg ggcaatctgg actgtacctt
     4561 ctgcctggat tgcgccaaag cgtgcactga ggacaacgtc ggcattctgg gccggaaacc
     4621 cgtgtccgag ttgtggaggg accgatccag ggctcccgcg accgactttg ctcgccggcc
     4681 cgatctggct gccatggcgg tagtgcttgt ggcggccgcc ttcatgaacg cctttgggat
     4741 ggttgccccg gtttatgggg tggagcggtg gatcgcggga tcgttgggca cggagtccag
     4801 gctactggtt ctggcggtgc tcttcggttt cggattggtc gtcgttccgg cgctactggt
     4861 ctccatcgcc ggctggatag ggaggtattt ggctggatct aggagaccgg tgatccaggt
     4921 cgcctcctac tacagcttcg cactggtacc gttgggcttc gggatgtggc tttcccacta
     4981 cagcttccac ttcctgaccg gcgccctgac cgtgttgccg gtggtccaga gccttctgtc
     5041 cgacctgggg ctcccgagga tcggtagccc tcaatggggg ctcgggccgg tcgtgccgtt
     5101 ctcctggctg atgcctgtgg agttgctctt cctggagggc ggacttctgg cctagctcgt
     5161 cgccgggtac aggatggctg ccagagagga gggggattcc ggaaagaccc tgacagcgtt
     5221 cctcccctgg gcgctgctct gcctggcgcc ttttgccgct ggggtgtggc tcatgcagca
     5281 gccgatggag atgcgcggca cctttgtggg agggtaatga catggcctcg acactactca
     5341 aacgatgggc cattcttcgg gcagtgctct cgatgttggc cgtgggtgtg ttgttgtcct
     5401 cctgcacgcc gcaagatgcg agattggcag gcatagacat gaagggggag ccggcaacgg
     5461 actttcgact gaccgggcat gctggcgata cggtgcagct ctcgagcctt cgtggccgag
     5521 tggtggtgct caccttcctc tacacctcct gcccggatac ctgccccctc attgccggca
     5581 agtttggagt ggtccacgac cggttgggcc agttggcgca gggcagttcc tttctcgctg
     5641 tgaccgttga tccggagcgg gatactccct acaggctgca ccaatatctc gaggcgcagc
     5701 agttgcaggg caaaatggat tttctcaccg gtagcaggcc cgatctagag aagatctgga
     5761 cggcctacca cgtgggcgtt cagcgaccac cagcgcacgc cgaggtcgga gggagcggcc
     5821 agggctatga ggtggcacat actgaagctg tgtacgttat cgacaaacag ggcaagctaa
     5881 gacgactctt caaggcagac ttcgaaccgg ctgcgctggc taaggatgtg gaactgctga
     5941 gccgagagta gcgccctttt cctgggatcc ggctactgac ggcaaggagg cctgttgtga
     6001 gtgacgagtc tatggcgctt ctgacctcat ggaatctaga gccggagttg gtggttggct
     6061 tgatggtcgc catcgggctc tacttcgtgg ggtggatgcg cctttcgcga cgcacatctg
     6121 aaggcgggcc gctgattcgg tggtgggcct ggtgctactg cagcggcatg gcggcgatag
     6181 ccattgctct gctctcgccc gtcgagacct tcagtgggct gttcttcttc gtgcatatga
     6241 tccagcacct gctgctgatg ctggtggcgg cgcctcttct ctggctcggg gcgccgatcc
     6301 tgcctctgat gtgggctctt ccgcggcgtg tcagggtcgg tttggggcgc atgttcgcct
     6361 gcggtcgccc tctgcatgcc ttgctgaact tcctgactaa tccgctggtt gcccaggcgc
     6421 tcttccttgc gacactggca ggttggcatg taccccccta ctacgacata gcgcaggggc
     6481 gcagcttggt ccacgagttc gagcacacca tgttcctcgg gacatcgttg ctcttctggt
     6541 ggccggtcgt tcatccctgg ggcggcaagc gcaggctgag ctacgaggcg ggcatcgcct
     6601 atttcatacc tcctatactc gtgagtaatc tgataggcgc gctactcact tttgccggcc
     6661 gtccactgta caccaactac ctgcatgtgc ctcgtctctg gggcatctca gtggtgctcg
     6721 atcaacagtt gggcggcctg ataatgtggg tgcccggtat gatcccctac gccattgcca
     6781 tcttcctcct cgtctctcaa ctgatgaagc agggagagct tcgagagaag gaggtcgaaa
     6841 ttgcagccgc gattcagcgg aagcagttgg gaactgaacc ggacgaaggt gcggaggaag
     6901 tcgatgccct ttagggttac cagtctgtat gtgatcgtgg tcgtggctgc gatggctgtg
     6961 ggcaccacgc cggccatggc caacggcggc accatacagt tatcgaatcg accagccggc
     7021 ccctatgccg tcactgtgta caccagcccg acgcccatcc gcgcgggtac gtcagatgtg
     7081 agcgtgatgg tgcagagggc gggatccgac gagttggtga acgacgcccg ggttgtcgtc
     7141 caggcacagc ccatcggcag agacggcggt ctgagcagct atcaggctac ccacgagcag
     7201 gctacgaaca agctcttcta cgctgccaat gtggcccttc catcggaagg acggtggcgg
     7261 attggggtgc aggttgaggc tgccgcgggc ttgggggagg tggacttcga aaccgacgtt
     7321 gccccggcgg ggctcctgga cagcccattg ctgctggtgc agttggtggc gctgccgctc
     7381 attcttgctg tgctttggtt gggaagccgg aagctgtcta aagggtaggg gcgacgcgcg
     7441 cgtcgcccct acaagttggt ccactgtccc ctacttcggc gtcggcatga aatgccctcg
     7501 ggggcgtttc cccagggtgt aggacgtgga ggtcttgagc cctgcgaagg aagtgaagat
     7561 cggtacgtat atgctgaaca ccagccagaa gaggaagagt tccttgattc gctggcccgg
     7621 ctttctcttc cccagtacca gttcgtcggt tccgagcacc ctccagaaca ccagcaggcc
     7681 cagcaggcta acgacggtga gcccgatcgc gaagagtgac ttcagcatat ctgtcgtcgc
     7741 gcccaactcg gagggatcga ccatggcgct ggcgacaatg aggccgtagc ttggcatcag
     7801 ggtgagggtg tcacgctcca atccgttcca gagccgcttg aacgcaagcc acggctttcc
     7861 ccgggaggtg gcttcggcga actcgtccag cggtccctcg cgccaacgag cactctgccg
     7921 gaacagcgcc tggtaggagc gccagtaggc gcgtaacctt tctttcagag tactctgagc
     7981 ccccatggcc attacggggt agacccacag cggaattgga aggcccctta ccttaaagtt
     8041 gcggccatac tgcattcgca accgcaactc aaccccggaa tcctggctgt gggagttggt
     8101 cggccagaag cccacactgt ggagggtggc gaggctggcc gcggcgccgg taaaggcaat
     8161 ttcgggcttt ttgatccacc ccagggtgag aaccgaggtg cctatggctg agagacggct
     8221 gtgcagcggc gccttccagt agtcggttgt gaacaggacc acaggctgat atgccctcac
     8281 atccctctta gggtcggtga cgtaatgata gaccaggcaa ggaatgtact gagggtcgaa
     8341 aagggtgtcg gcgtcgaaat cggccacgat ccacttattg ggtgggaacc ccttgcggag
     8401 ggcctccctg tataaaacgc ggccggtgta gttcattgct gtggacggtc ccggcagggt
     8461 gttcggctca tctgggtgct tgatttgccg tattgccacc cggtccgcat actcctctgc
     8521 cagcttatct accagcggag gggtgtaggg gtcatcctcc tcggtggcgt acagcacctc
     8581 cacgttcacc atcttgccct gctcttttgg ccaattggat gcgtagatgg aatccagtgt
     8641 ggccctgagc atatccaggt tcttctcgtg gtaggctcgg atcatcaggg tcactcggca
     8701 gttgcgccag tcggggatat cctgctctag agcctccagc cgtgcgagcc agtcgtcctg
     8761 ctgggatttg agaaccctgt gacgattgat gagagctttg ggcccgtacc atgaagctcg
     8821 ggcggagaaa tagacgctgg cggctaccac caggatgagc cctaatattg gtgatagcag
     8881 cgttacccaa atcggtgaga gggctaccaa ccaactcacc acgcccggga ctctctccca
     8941 gagtcggtcg gtgactttgg gaaccctttc agtgtatgtc tggacggcct ctaactggcc
     9001 aagcctgtcg cgtagcatat gtactcccgc aggtcgttat gcccgtcaac cgggacgggc
     9061 cggcagcagg tcgtcgttac ctgtgctgcc ggttaagcga gcccttgacg ctcgcaagtc
     9121 gcagtggttc ttgctcactc cttctccgtt gcgggcctga ctagggaggt gtagaacagt
     9181 cacaaacggg ccgcaacggg gtgggggcaa gaaacatgcc aatttgaacc aacgctggca
     9241 tcttcagttt gtcctggtga aaacagccca agtattgatg aggtggtgta aaggggctaa
     9301 ggtgtagcaa gggttccacc cttgaacggg gggggcaggc tctgctatca tcgtaaccga
     9361 gtcaccattc tatacctcag aacccttcca agtccaaatt tgggttcgcc gttcttcaag
     9421 actcgtgccg gtggacgggt agacggaagg tcgccatggc gtttcctgca gtcattgtgg
     9481 gaccgctcct ccgtctgggg ctgttggcac tgctgctcaa ccccatgact gccctccccg
     9541 ctgcccatgt ttcccccgcc ccgcttgacg tcaaggtcgg ttcggttgcg gatagttccg
     9601 gccagccgac cactaagaag ttgctgttag tggacgggaa gcccttcgcc ccggtgggtg
     9661 tcacctacca ctttacctac cacagggata gctgggacga cgaccttcag gcgatgcacg
     9721 acatggggtt gaacagcgtg cgcatcgatt tctcctggag ggacatagag ccgatacttc
     9781 cgggccatta tcgctttgga gtgctggacg agttcctgga tcgtgccagc cagcatgggc
     9841 tgtacgtggt tcccgtcttc tcccacacca cgagggactt caatacgccg ctctggttct
     9901 ggcccgacta cgatagttgg agttccgtgg atcagaacgg gcagcgccct ctggacgatc
     9961 tccccagcat cagctacccc gagtatcgac gccgattgag cagctacatg gcggccacgg
    10021 ttcagcacat caagcagcac cctgcggtgc tcgcctatca gttgttgaac gagccacgat
    10081 acgatctgaa gagactgtcc gattacaacc cgtacacggt tgccgccttc cgcgtctggc
    10141 tgcgcgagca gtactccacg atagatcagc tgaacgacgc ctggggtacc ggctacaagt
    10201 cgtttgagga gactcagccg gcccgcgtgg ctgatgacga tccccccaag gatggtccgc
    10261 tgctgaagca gtggagcgat tggcgacagt tcagctacga taacctggcc gactttattg
    10321 gggagttggc gacgacggtg aagtctgcgg accccggcca ccccgtcatc gttgcggaaa
    10381 tggcctggtg gtggtgggga gagcagccct acagcggcgt gtcgccgctt cacatctaca
    10441 aggatgcgga catcgtcggt tacgacctct accccgatag tatcgaggac gccagctatt
    10501 tcctgctcac ctccgatatg ctctcccgct actggcagaa accggtgtgg gttatggaga
    10561 tgaacagcaa ggacggcgac cccaccggcg aggaaaccca gaagttcgtc tcttcggcga
    10621 tggacggtgg ggcaactggg gtcttctact tcgagtggca agacgattgg agggatggcg
    10681 gcaactacgg cgtgctggac tccaggggca gaaggaggac ccagtacggt gccttgacct
    10741 cggccgtccg ctggctacga cagaaggagg agagcctggt tatggcgccg actcccaagc
    10801 ccgacgtcta tctcgtgtgg ccgagcggtg aggtagggca ggtcaccggc gcggaatcgc
    10861 ccgcatggga catttacgcc accgcgcggc gcatcgtgga gggcggtctg cgcatcgggt
    10921 tggtcgcgga ggaactgatg cacgccgtgg atccctccaa gctgcttact cttcgggacg
    10981 ggcgcctgga aaacggcaag gcccccactc ccggccccac gccccaatcc cccagccgcg
    11041 gctttctgat tagcgattag cccctgctgt gctatcattg gtgctagaac atcctggggg
    11101 tatcgcttcg tgcagatcgc cataatcggt cttcccatgt cgggcaagac cacagtcttc
    11161 aacgccctga cgggtgctcg ggcaaagacc ggtgccta
//
LOCUS       DATALS010000355         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_172883, whole genome shotgun sequence.
ACCESSION   DATALS010000355 DATALS010000000
VERSION     DATALS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172883"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="VHS06_07975"
     CDS             complement(<1..1676)
                     /locus_tag="VHS06_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HEX2988091.1"
                     /translation="MADRLLLTDLSALRFLLLIPVIMALYMVRSRYRRRQISSTMLWR
                     SIRRDLEARQQLRLPPLSLLLLLELLAILAGTAALLKPALPAGDRSHLVLLVDVSSSM
                     QATDVSPSRFGVAVQQARAAIQQQKEGEIASIVAAGPSPKLLASGADKTELLATLDHL
                     QPGSASVDAGAALRLAEALILGTGGKGSALFLSDGLFGPNFQAPRLSIPVEYQPIGAS
                     GDNQGITAVDVRPYLDGSGRWSAFARVVNYSGNPANVTATATADGLLLDSRPLGLPAG
                     GSSELTFDLPSGTKAFAVKLDAGTSFAADKQVELRIDTPYQRKLLLVSKDAGPIARVL
                     SSVPGFQLSSIKPEEYTGAAGADVVVLDGFVPPKLPDAEVLLLDPPADAPGFSAKAIG
                     SEATVLRSSPRNPLLNSVDLQSLRLGQTTQLETPEWARNVAEGPTGPLILQGDQAGRR
                     IVIFAFNWQLYDLPRMQAFPLLISNALSELNPVALPREIQPGQSVLLRPMGDAREATV
                     GLPDGSSRQVSLKDGAVSFGDTQQVGRYEVRWKGATLGELSSSFNVNLASP"
BASE COUNT          309 a          558 c          566 g          302 t
ORIGIN      
        1 gggctcgcca ggttgacgtt gaagctgctg gacagctctc ccagcgtcgc tcccttccac
       61 cgaacctcat atcgccctac ctgttgggtg tccccgaagc tcacggctcc gtctttcagc
      121 gaaacctgcc gggaggagcc gtccggcaga cccacagtag cctccctggc atcgcccatc
      181 ggcctgagca gcacgctctg gcccggctgg atctccctgg gaagcgctac cgggtttagc
      241 tccgagaggg cgttagagat cagcaggggg aacgcctgca tgcgaggcag gtcgtacagc
      301 tgccagttga aggcgaagat cacaatcctt ctgccggctt gatccccctg gaggatcagc
      361 ggccccgtgg ggccctctgc cacatttctg gcccattccg gcgtctccaa ctgggtggtc
      421 tgtcccaggc gaaggctctg caggtccacc gaattcagta gcgggttacg agggctgctt
      481 ctgagcaccg tcgcctcgct ccctatggcc tttgccgaga agccgggcgc gtcggcagga
      541 ggatccagca gcagaacttc tgcgtcgggc agcttcggcg gaacgaagcc gtccagcacg
      601 accacgtcgg cccctgccgc ccccgtgtac tcctcaggct tgatggaaga cagctggaag
      661 ccgggaacgg aggagagaac ccgcgcgatg gggccggcat ctttagaaac cagcagtagc
      721 ttgcgctggt agggggtatc gatccgaagc tccacctgtt tgtcggcggc gaaggaagtg
      781 ccggcatcca gcttcacagc gaaggctttc gtcccagatg gcaggtcgaa ggtcaactcc
      841 gagcttcccc ccgcgggcag tcccaggggg cggctgtcca gcagcagccc gtcggcggtg
      901 gcggtggccg tgacgttggc cggattcccc gagtagttga cgaccctggc aaaggcgctc
      961 caccggccgg aaccgtccag atagggccgt acgtccacag ccgtgatccc ctgattgtcc
     1021 ccgctggcgc cgatgggctg gtactccacc ggtatcgaga gtcgaggagc ctggaagttg
     1081 gggccaaaca gcccatcgga caggaatagg gcactgccct ttcctccggt gccaaggatc
     1141 aatgcctctg ccagtctgag tgcggcaccg gcgtccacgg aggcagaccc aggctgcagg
     1201 tgatccaacg tggccagcag ctcagtctta tcggccccgc ttgccagcag tttggggctt
     1261 ggtccggcag cgacgatgct ggctatctcg ccctccttct gttgctggat tgcggccctg
     1321 gcttgctgca ccgccacgcc aaagcgagag ggggagacgt ccgttgcctg catgctggac
     1381 gagacgtcga ccagcaggac gagatgggat ctgtccccgg ccggcagcgc tggcttcagc
     1441 aacgccgcgg tgcccgccag gatggccagc agttccagca gcaatagcag actgagcggg
     1501 ggaagcctca gctgctgccg ggcctccagg tcgcgccgga tacttcgcca cagcatggtg
     1561 ctggagatct gccgccgccg gtatcgggat cgcaccatat agagggccat gatcaccggt
     1621 atcagtagca ggaagcgcag cgcggacaga tcggtgagca gcaagcggtc agccatcagg
     1681 agttagccgc atagaattgt agggcggggt cttgtgcccc gcccgtacca cgtgc
//
LOCUS       DATALS010000356         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_172903, whole genome shotgun sequence.
ACCESSION   DATALS010000356 DATALS010000000
VERSION     DATALS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172903"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..497
                     /locus_tag="VHS06_07980"
     CDS             105..497
                     /locus_tag="VHS06_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2988092.1"
                     /translation="MGQRRVLIVEDEPDNQEIVRAVVEDIIGCSATLACDGLEAISKA
                     REDPPALVLLDLMLPKMDGYEVARLLRQEDGTRRVPIIAITALARPSDRIKAMESGCN
                     DYVDKPFDLEALEQKIRARVFLSEEGSV"
     gene            762..1556
                     /locus_tag="VHS06_07985"
     CDS             762..1556
                     /locus_tag="VHS06_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HEX2988093.1"
                     /translation="MIPPLPLGEGRGEGMALKSFAIEYGLGVDLHGQDPTKAAVKAVK
                     NAVERISLPGMRQVAGVTDLDKQVAVEILLGVPAPMVDMVDLDAVTSALPFGRRSVSV
                     SAGGLLAPNGVIVPAMGDTTDQAVLVVAAVTLKVEVPETVHQAGGIILLGDQVVLRRT
                     PKSEYLFPKGHIDPGETPEQTAVREVAEETGLDARIIAPVGQASFSYQGDNYVVDFYL
                     MTATAQLPDWQQHLATDVVTVPVEEVAGLLSFESYRRLWARAEAMI"
     gene            complement(1517..2503)
                     /locus_tag="VHS06_07990"
     CDS             complement(1517..2503)
                     /locus_tag="VHS06_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2988094.1"
                     /translation="MKDSEIQPKRNGTISDHTNGHLWKKAVLPASLAAGGLGTALVGL
                     AAYTAVRLAKPDRRFGNGEPPDVSYETVSFPSTDGLRLSGWFLPAGEKADGLVLCHGF
                     HTGRRELLPVAMALRKLGHNVLIFDFRGHGNSEGRWSSCGQLETRDLEGAVRFICRRP
                     EVYEDRVGVIGYSMGAATAIMTAARVREIRAVVADSPFATLREVLESTFRNHYHVPCF
                     PILSMAVWINGQIVGASMSSTRPLDEVAKISPRPLMLIHGEADRIVPMSESLLLHEAA
                     GEPKELWTVPGVDHVEARLHNLDGYVARVDAFFKKWLAGDQIMASARAQSRR"
     gene            2784..>3493
                     /locus_tag="VHS06_07995"
     CDS             2784..>3493
                     /locus_tag="VHS06_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX2988095.1"
                     /translation="MHPRLAAAVAAGQLATLLSRRLGIGGGTTAPGHLVRVLDPGAVS
                     SIAAGIPSGSIVVTGTNGKTTTSRMISTLLRRAGLSVMHNRSGANLIAGITSTLVNGS
                     SLAGHPRADVGLFEVDEATFPAALQELRPRLCVLTNLFRDQLDRYGEVDYLSRIWRDA
                     LLRLPESSTVLLNADDPRIAGLAEGLTCRVLYYGVEDRSLAGPSLSHAADSRFCLRCG
                     APYNYDVAFYSHIGHYRCL"
BASE COUNT          601 a         1092 c         1098 g          702 t
ORIGIN      
        1 agcacccaag actgtgttga ccatgtttgc tgaaccacct acactgatta aacactcagg
       61 caggccgtac tgcgagtcgc ctttccaggc gtggggaggt gtctgtggga cagcgacgag
      121 tcttgatcgt cgaggatgaa cccgataacc aggaaattgt gcgcgctgtc gtggaagaca
      181 tcatcggctg tagtgctacc ctggcgtgtg atggcttgga ggcgatctcg aaggcgcggg
      241 aggatcctcc cgccctggtg cttctggatc tcatgctgcc gaaaatggac ggatacgagg
      301 tcgccaggct gctgagacag gaggatggca cccggagggt gccgatcatc gcaatcacgg
      361 ccctggccag accctccgat cgcatcaagg cgatggagtc tggctgcaac gactacgttg
      421 acaagccttt cgacctggag gcgttggagc agaagatccg cgctcgggtg tttctctcgg
      481 aggagggatc ggtttgacgg ggcaggacct acttctggtt gtggggttgg cgggcacggc
      541 cctgggcatt actctgattg atcttctctg gagcggctgg cggcatcgcc gtcggatggc
      601 ttgtctgcgc gcctccggag tgaccggcgg ggccagcggc aataatgccg gaggctctcc
      661 tgagttgtgg gaccgtctct ccgctatcga ggctgccggg tctaatgctc cctctgggag
      721 aagccaactt gatgcgccct ctcaatttgg gagaggctga tttgataccc cctctccctt
      781 tgggagaggg tcggggtgag ggaatggctc tcaagagctt tgctatcgaa tacggcctgg
      841 gtgtggatct tcacggccag gaccctacta aggcagccgt caaggcggtg aagaacgccg
      901 tggagcgcat ctcactcccc ggcatgcggc aggttgccgg cgtcaccgac ctggataagc
      961 aggttgccgt ggagatcttg ctgggggtgc ctgcccccat ggtggatatg gtggatctcg
     1021 atgctgtgac ctcggcgcta cccttcggcc gccgttcggt ctccgtgtct gccggtgggc
     1081 tgcttgcccc caacggggtc atcgtgccgg ccatggggga caccactgat caggccgtcc
     1141 tggtggtggc cgccgtcacg ctgaaggtcg aggtgccgga aaccgtccac caggcgggcg
     1201 gcatcattct cctcggcgat caggtagtgt tgcgtcgtac gcccaagagc gagtatctct
     1261 tccccaaggg gcacatcgat ccaggggaga cgccggagca gacggccgtg cgggaggttg
     1321 ccgaggagac ggggctggat gccaggatca ttgccccggt ggggcaagcc tccttcagct
     1381 accagggcga caactacgtc gtggacttct atctgatgac tgctaccgcg caactcccag
     1441 actggcagca gcatctggct acggatgtgg tcaccgtgcc ggtggaggag gtggcgggac
     1501 tgctcagctt cgagagctac cggcggctct gggcgcgagc cgaggccatg atctgatccc
     1561 ccgccagcca cttcttgaag aaggcgtcta ccctggccac gtatccatcc aggttgtgca
     1621 gccgagcctc cacgtggtct actccgggga ccgtccaaag ctcctttggc tccccggctg
     1681 cttcgtgcag cagcaggctc tccgacatag ggacaatgcg atcggcctcc ccatggatca
     1741 gcatgagcgg tctgggggag atcttggcaa cctcgtccag ggggcgagta ctggacatac
     1801 ttgcccccac tatctgtccg ttgatccaca ccgccatgga taggatcgga aagcacggaa
     1861 cgtggtagtg gttgcggaag gtgctctcca gcacttcccg taaggtggcg aaggggctgt
     1921 ctgccaccac tgccctgatc tctctcaccc gtgccgccgt catgatggca gtggcagcgc
     1981 ccatcgagta accgatcaca cctacgcgat cctcgtacac ctctggtcgc cggcagatga
     2041 agcgcaccgc tccctcgagg tctcgggttt ccagttgccc gcaagaactc catcgtccct
     2101 cactgttgcc gtggccccgg aaatcgaaga tcaggacgtt gtgtccgagc tttcgcagcg
     2161 ccatggccac gggcagcagt tcacgccggc cggtatggaa cccgtggcag aggaccagac
     2221 cgtccgcctt ttcccctgcg ggtaggaacc accccgacag tcgcagtccg tccgtggaag
     2281 ggaagctcac cgtctcgtag cttacatcgg gtggctcacc gttgccgaag cggcggtccg
     2341 gcttcgccag tcgcacagcc gtataggcgg ctagaccgac cagcgccgtg ccgagccccc
     2401 ctgcagcgag ggacgcgggc aacaccgcct tcttccatag atggccgttt gtgtggtccg
     2461 agatagtccc gttgcgcttt ggctgtatct cagaatcttt cattgcgcta gctcctaaac
     2521 gaagacattc aacgaaagat aatggccctc tcaggccatg atatcaacca ctttgcagtc
     2581 tgtccatacg tgggggatgg gggatggggg gtaggggatg ggggctacga agtagcgagc
     2641 atggactagg aaggcacagc ggcacggagc cacggcgaac ccccaacccc tatcccccat
     2701 cccccatccc cctcgtcggt ccttttcttg ctctggcccg atcgatcgac tatcatctgc
     2761 ccagtcaagg ggggagatcg tctatgcatc cgcgcctggc cgcggccgtt gccgcgggtc
     2821 aattagccac tctgctcagt cgtcgcctcg gcatcggcgg ggggactacc gcgccgggcc
     2881 acctggtcag ggtgttggat cctggcgccg tttcttccat agccgcaggg atccccagcg
     2941 gctccattgt ggttaccggt accaacggta agaccaccac atctcggatg atatccaccc
     3001 tccttcgcag agccggtctg agcgttatgc acaaccgctc cggtgccaac ctgattgccg
     3061 gcatcacctc caccctggtc aacggctcat cgctcgcggg ccatcctcgg gccgacgtgg
     3121 gcctgtttga ggtcgatgag gctactttcc ccgcggccct tcaggagttg cgaccgcggt
     3181 tgtgcgtgct caccaacctg ttccgcgatc agttggatcg ctacggcgag gtggactacc
     3241 tgtcgcgcat ctggcgcgac gccctgctgc ggcttccgga gtcgtcgacc gtgctgctga
     3301 acgccgacga tcccaggata gccgggctgg cggaaggtct cacctgtcgg gtcctgtact
     3361 acggggtcga ggaccggtcg ctggccggac catcgctctc ccatgccgcc gattcccgct
     3421 tctgcctgcg ctgcggggct ccctacaact acgacgtcgc cttctacagc cacatcggtc
     3481 actaccgctg cct
//
LOCUS       DATALS010000357         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_395362, whole genome shotgun sequence.
ACCESSION   DATALS010000357 DATALS010000000
VERSION     DATALS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395362"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VHS06_08000"
     CDS             <1..770
                     /locus_tag="VHS06_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988096.1"
                     /translation="AQEREFCMSNPYASFCEDFYINMRLGSSMALPHSRETVLHFFER
                     IQKQFPAMTRFRRSEGEISLEEERDRDGYRWLSLESKRLSSGQVNPSSIEQAMAYHQF
                     VLEQAPYQLGLSMLEIEYVDTLFGFDLSFSGNHDEIIAETLLAQSPLASLSEEAGARP
                     VDFQPSFTVSLSDDCRLQARLDVITRTNSYQVRTGDYSEETISVYLIVRRYWGDRPQI
                     ALERMVRQLADKADTLCGSCVIPRILRPISEAIASRS"
     gene            880..1059
                     /locus_tag="VHS06_08005"
     CDS             880..1059
                     /locus_tag="VHS06_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988097.1"
                     /translation="MLQQAAQSAGRLLEHLSEHAAGGQGEGLTSGAHTAQAGNPLESA
                     IASLRSLVAALHKES"
     gene            1173..1805
                     /locus_tag="VHS06_08010"
     CDS             1173..1805
                     /locus_tag="VHS06_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988098.1"
                     /translation="MFTIILAMGLWFLYDGFVAYPAANQKYDELSKELNAMEKDPRAE
                     EAKKLSVTIQLKSMSHHDDFSILLQKILGFTLPPIGIGLLVYWIRKSRGEIRLENGVL
                     TLPGSPAVPLAAIDELDKELWERKGIATVYYTLDNGVTGKFLLDDFIYQAHPVRAIVK
                     AIEDEIQFQDELLAKAEQDRLEQEKLSRERQEQEEAKRLAADRPAEQGRY"
     gene            2000..2836
                     /locus_tag="VHS06_08015"
     CDS             2000..2836
                     /locus_tag="VHS06_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEX2988099.1"
                     /translation="MSMKFVLVLLAGFLTFSSSAFADPDPNFYIFLCFGQSNMDGAAK
                     PEEQDKQGIDPRFQVLWTINSPNLGRTAGNWYPAVPPLCRTYSGISPADYFGRTMVAN
                     LPASIRVGVINVSVPGCDIRLFNKDNFEPYAATAPDWMKGIIKEYHGNPYTHLVGMAK
                     LAQKDGVIKGILFHQGETNTSQKDWPAKVKLVYDNLLKDLDLKPDSVPLLAGEVVNKD
                     QGGACAIHNAIVAELPKTIPNSYVVSSAGCTAAPDHLHFNAAGYRELGNRYAVQMLSQ
                     LG"
BASE COUNT          590 a         1086 c          756 g          499 t
ORIGIN      
        1 gcgcgcagga acgagagttt tgtatgtcca acccctacgc gtcattctgc gaggacttct
       61 atatcaacat gcgccttggc tcgagcatgg cgttgcccca ctcgcgtgaa acggtgctcc
      121 acttcttcga gcgaatccag aagcagttcc ccgccatgac ccgcttccgc cgcagcgaag
      181 gcgagatcag cctcgaagaa gagcgagacc gcgatggcta tcgctggctg agcctggagt
      241 ccaaacgcct ctccagcgga caggtcaatc cctcctccat cgagcaggcc atggcctacc
      301 accagttcgt cctggaacag gccccctacc agcttggcct gagcatgctt gagatcgagt
      361 acgtcgacac gctcttcggt ttcgacctct ccttctccgg caaccacgac gagatcatcg
      421 ccgaaaccct cctggcccaa tcgccccttg ccagcctcag cgaagaagcg ggcgcccgcc
      481 ccgtcgactt ccagccctcc ttcaccgtct cgctctccga cgactgccgc ctccaggccc
      541 gcctggacgt aatcacccgc actaacagct atcaggtccg caccggcgac tattccgaag
      601 agaccatcag cgtctacctg atcgtccgcc gctactgggg cgaccgcccg cagatcgccc
      661 tggaacgaat ggttcgccaa cttgccgaca aggccgacac cctctgcgga agctgcgtga
      721 tcccccggat ccttcggcca atctccgaag ccatcgcctc gcgctcctga tgcccgccac
      781 aagcacccga ccggcacgcc cgcgtcgcgg gggctttgcc ctcgccatgc tccccagtac
      841 aattccgcca tgggccagca ggatcgcgcg gatgaggcta tgctgcagca ggccgcccag
      901 tcggcaggca ggctgcttga acacctctcc gaacacgctg ctggcggaca aggcgaggga
      961 ctgacgtcgg gcgcccatac cgcccaggcc ggcaatccgc tggagtcagc gattgcatcc
     1021 ctgcgcagcc tggtcgccgc attgcacaag gagtcgtagc gcaaatgagc accccacccc
     1081 aagcgcccaa caccgcgccc cttagccccg ttgcccccac aacccccatc tccgcccgcg
     1141 caggcagata ctaccgcaac gcccgctacc tgatgttcac catcatcctc gccatgggcc
     1201 tctggttcct ctacgatggt ttcgtcgcct accccgccgc caaccagaag tacgacgagc
     1261 tctccaagga actcaacgcg atggagaagg atccccgcgc cgaggaagcc aagaagctca
     1321 gcgtcaccat ccagttgaag agcatgtcgc accacgacga cttcagcatc ctcctgcaga
     1381 agatcctcgg cttcaccctt ccaccaatcg gaatcggcct gctcgtctac tggatccgca
     1441 aatcgcgcgg cgagattcgc ctcgaaaacg gcgtcctgac cctccccggc tcgcccgccg
     1501 tccccttggc cgccatcgac gaactcgaca aggaactctg ggaacgcaaa ggcatcgcca
     1561 ccgtctacta caccctggac aacggcgtca ccggcaagtt cctcctcgac gacttcatct
     1621 accaggcaca ccccgtccgg gccatcgtca aggcgatcga ggacgaaatc cagttccagg
     1681 acgagctgct cgccaaggcc gaacaggatc gcctggagca ggagaaactc tcgcgcgaac
     1741 gccaggaaca ggaagaagcc aaacgcctgg ccgccgacag acccgccgag caaggccgct
     1801 actagcccca accttacccc ttatcccttg ggtcgccacg tgccggtaaa ccgtctattg
     1861 cgtcgcaaac gcaagcctcg tagactccca ccgcagttcc cgatcccgca gattggttct
     1921 gcccaatagg cgaggagccc tgtacacgcg gcggccggag aaccgcgggc tcatcggccc
     1981 aactctggag acacctgcta tgagcatgaa gttcgtcctg gtccttctcg ccgggttcct
     2041 cacgttctcc tccagtgcat ttgccgatcc agacccgaac ttctacatct tcctctgctt
     2101 tggccagtcc aacatggatg gtgccgcaaa gcccgaggag caggacaaac agggtatcga
     2161 tccgcgcttc caggtcctct ggacgatcaa cagcccgaat ctcggccgca ctgccggcaa
     2221 ctggtacccc gcggtgccac cgctctgccg gacctactcc ggcatctccc ccgccgacta
     2281 cttcggccga accatggttg ccaacctccc ggccagtatc cgcgtcggtg tcatcaacgt
     2341 ctccgtgccc ggctgcgata tcaggctgtt caacaaggac aacttcgagc catacgccgc
     2401 cactgccccg gactggatga agggcatcat caaggagtat cacggcaacc cttacaccca
     2461 tctcgtcggg atggcgaagc tcgctcagaa ggacggcgtc atcaaaggca tcctcttcca
     2521 tcagggcgaa accaacacga gccagaaaga ctggcccgcc aaggtcaagc tcgtctatga
     2581 caacctgctc aaggacctgg atctcaagcc cgattccgtc cccctgctcg ccggcgaagt
     2641 cgtgaataag gatcagggcg gcgcctgcgc catccacaac gccatcgtcg ccgagcttcc
     2701 caagaccatc cccaactcct acgtggtctc ctccgccggc tgcacggccg ctcccgacca
     2761 tctgcacttc aacgcggccg gctaccgcga gctcggcaat cgctacgccg tgcagatgct
     2821 ctcccagctg ggttgagcag ccgaccgcgc aatatgggca gacgctgtcg gtcacgattg
     2881 catccttggt ccgggcgtgc ttcttgcggg tctgcccgct gaagggctga t
//
LOCUS       DATALS010000358         3440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_222749, whole genome shotgun sequence.
ACCESSION   DATALS010000358 DATALS010000000
VERSION     DATALS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222749"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..521
                     /locus_tag="VHS06_08020"
     CDS             132..521
                     /locus_tag="VHS06_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2988100.1"
                     /translation="MDSRAKEPVVIREQDVKPFVSPLPHHRTIRVLLSPQKSPVATGV
                     AFGVVEVGPGQVSPPHYHETEQEAWYVISGSGTIRVGDKTFPVEAGTVVVSPPQLEHQ
                     ITNPGPEVFKAIFMFSPAGPETALFAE"
     gene            complement(878..1561)
                     /locus_tag="VHS06_08025"
     CDS             complement(878..1561)
                     /locus_tag="VHS06_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX2988101.1"
                     /translation="MEDLSERSTTQPQPEQQPAEPSNQAPEQSAETQATETVAAEQPS
                     AEPVSASEATETKPTEAPKATEQPAAPQKPKEPTGPKLSELRPGTVVEGKVTRVERYG
                     AFVNLGLAEKRDGLIHISELASYRVRRVEDVVNVGDEVRARVVSVDPARGRIALSLNE
                     VPLEKYNETTPSNEPTLTAMALAFQEAYSRKKESDRQAQGGTGREDKGSRARREQQQM
                     IERYRNSSR"
     gene            2224..3324
                     /locus_tag="VHS06_08030"
     CDS             2224..3324
                     /locus_tag="VHS06_08030"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX2988102.1"
                     /translation="MQRKQIMGLLVSTAAAVALFAAACSSPAAPAPTQAPAAPKAAEP
                     TKAAAAPAATTAPAAPAASKTAFPEKGKSIMMTVPFAAGGSTDIGARLLATGMEKALG
                     TSVQVQNRAGAGSQSGLTDIVKSKPDGYNIGFANLPTANLIYSDPDRKAVFSGKDFIP
                     VGVQVVDPSAIAVKADSPYKSVKDLVEAAKKAPETIKFGSDGPMTDDHLGIFQLEQAS
                     GAKFAMVGFDGSAPNMSALLGGHIDATFGHVGDFLSPAKAGQVRVIGIADKEKSKFFP
                     EAQTLQEQGYNITNSSSRTLVVPAGTPQDVVDVLSAAMKKAMDEPEHIQKMQDAGLTL
                     RYMNSKDARAYWDSMDAPAKALVDQVRAAGKK"
BASE COUNT          622 a         1098 c         1037 g          683 t
ORIGIN      
        1 aagaacagcg ccatcaccag gaacaccagc cccaccccca ccatctgccc ctttcccatg
       61 gagctgaagc cctctgatag ctgctgcagg accccaccgt agtagagaga agacagttgg
      121 gggtagtaca gatggacagc agggcaaagg agcccgtcgt cattcgcgag caggacgtga
      181 agcccttcgt ctctccactt ccccatcatc gcaccatcag ggtgctgctt tcaccgcaga
      241 agagcccggt ggcaaccggc gtggccttcg gtgtagtgga agtgggcccg ggtcaggttt
      301 ctcctcctca ctaccatgag accgagcagg aggcctggta cgtgatctcg gggagcggca
      361 ccatcagggt cggtgacaag accttcccgg tggaggcagg gacggtggtt gtgtcccctc
      421 ctcagttgga gcatcaaatc accaatccgg ggccggaggt cttcaaggcg atctttatgt
      481 tctcaccggc cggccctgaa acggccctct tcgcggagta gacggcacgg cggtaaatcc
      541 ccatcgggcg ggtcgataag acccgcccgt tcccgttctt ggagttgtgc aagccccgat
      601 cggagaaccc gatagcgggg tgccgacctg gaagttcttg ttgatgggtg atgatggcga
      661 cgatgatggc cttgccattg gcggccggct cgcaacgggc accatcgcct tatggcaggc
      721 tattcagggt ataaaacacc cagcgctgct tgctagcagg agctaccagg agcgttagtg
      781 agctgttcag ttggctgaac gtacaggagc cagaagaccg ggaggttatt cccggtgcgg
      841 gaggcttcgc ggtccagtat ccggctgttg gggcagccta gcgtgacgag ttgcggtatc
      901 tctcgatcat ctgctgctgc tcgcgtcgtg cacgactgcc tttgtcctca cgacctgtgc
      961 caccctgagc ttgccgatcg ctctccttct tgcggctgta ggcctcctgg aaagccagcg
     1021 ccattgccgt aagggtgggc tcgttgctgg gggtggtttc gttgtacttc tccaacggca
     1081 cttcgttcag cgacagcgcg atcctgccac gtgctggatc cacggagacc actctggccc
     1141 gcacctcgtc gcctacgttc acgacgtcct caaccctgcg aaccctgtag gacgccagtt
     1201 cagagatgtg gatcagtcca tcgcgcttct cggccaggcc cagattgacg aaggctccgt
     1261 accgctccac gcgggtgacc ttgccttcca cgaccgttcc agggcgcagc tcggagagct
     1321 tgggtccggt gggttccttg ggcttctggg gggctgccgg ctgttcggtc gctttcgggg
     1381 cttcggtcgg cttggtctct gttgcctcgc tcgcggatac cggctccgcc gacggctgct
     1441 ccgccgcgac ggtctcggtg gcctgagtct ctgcactctg ctcgggcgct tggttgctgg
     1501 gctcggcagg ttgctgctcg ggctggggtt gggttgtgct cctctcggac aggtcttcca
     1561 agtctcggac tccaaaatcg ggattggcga cggcacatct ctccgtcgcg gaactactat
     1621 tatagcagcc tccgcaccga gacggcttcc ctacttctgt tgtgaggggg agccttgatg
     1681 atattggaag ttgacagctc catggcaaaa gaatagtcta ggaaacaagt acgacgtttg
     1741 cttttaccgc gatggggcgg ctccacaaag ccggagcgtt gcccaccctt ttcagtgtct
     1801 cgtggcgatc ttcgatcgcc tgcctccgac ggatcaatgt agggttcggt gattctctga
     1861 accttgcacc gctcgtgccg ggagactgct gagtaagggg caactctccc ttctctgttg
     1921 cctttctgat tccgagaggc gcgcccgttg cccgcaaacc ggattctaga agtgcctcag
     1981 agatgccccc aaaggccgtc ggggtcgatg acgccccgag agttgccatg gcgagcctcg
     2041 ccatggccgt tcattgagcc aaaggaggtg gcaagcgaac cacatccatg cagcgagtgc
     2101 tgctgccaac ggcgcgtgat tccggcgccg gcacattctg tttctccgac cgtttcacat
     2161 ccggggtggc ccgatctcaa cctgagcgag ataattgcca ctgcgtagtt aggagataat
     2221 ccaatgcaac gcaaacagat catgggcctc ttggtttcaa ctgcagcagc agtcgcgctc
     2281 ttcgcggcag cctgctcttc gcccgcagcc cccgcaccga ctcaggctcc cgctgccccc
     2341 aaggcagccg agccgaccaa ggccgctgct gcgcctgccg ctaccaccgc tccggcggcc
     2401 cctgcggcat ccaagactgc cttccccgag aagggcaagt ccatcatgat gacagtcccg
     2461 tttgctgccg gcggcagcac ggacatcggt gcccgcctgc tggctaccgg tatggagaag
     2521 gcgctgggca cctcggttca ggtccagaac agggccggcg cgggctccca gagtggcctc
     2581 accgacatcg tcaagtccaa gcccgacggc tacaacatcg gtttcgccaa cctgcccacc
     2641 gccaacctga tctacagcga ccccgatcgc aaggccgtct tcagcggcaa ggacttcatc
     2701 ccggtgggcg tccaggtggt ggacccctcc gccattgcgg tcaaggccga ctcgccctac
     2761 aagagcgtca aggacctggt cgaggctgcc aagaaggcgc ctgagaccat caagttcggc
     2821 tcggacggac cgatgaccga cgaccacctg ggcatcttcc agttggagca ggcctccgga
     2881 gccaagttcg ccatggtcgg cttcgacggt agcgccccca acatgtccgc actgctgggt
     2941 ggccacatcg atgcgacctt cggccacgtc ggtgacttcc tttccccggc caaggcgggc
     3001 caggtgcgcg tgatcggcat tgccgacaag gagaagagca agttcttccc agaggcccag
     3061 acgctgcagg agcagggtta caacatcacc aactcctcct ctcggactct ggtcgtgcca
     3121 gccggcactc cccaggatgt cgtggacgtt ctgagtgccg cgatgaagaa ggcgatggat
     3181 gagcccgagc acatccagaa gatgcaggac gccggcctca ccctgcgcta catgaactcc
     3241 aaggacgccc gggcctactg ggactccatg gatgccccgg ccaaggccct ggtggaccaa
     3301 gtccgagcgg caggtaagaa gtaacaactc cttccacagc gcgatcacaa gagccagggg
     3361 gtcggcaatc gccgaccccc ttcgtgtttt tcggcttgtt cgcattccgg tgccgtgccc
     3421 gtggacatgg actataatcc
//
LOCUS       DATALS010000359        10372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_39306,
            whole genome shotgun sequence.
ACCESSION   DATALS010000359 DATALS010000000
VERSION     DATALS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39306"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2856
                     /locus_tag="VHS06_08035"
     CDS             <1..2856
                     /locus_tag="VHS06_08035"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2988103.1"
                     /translation="AHLAVEAGDYAGARAVYEESLATTRELGFNYEIVQAITGLANLA
                     SVEGDHVAAARLYDEVAELHQDLGEEVRWLQFRGIAANHRGEHAEARTLLLQALGMFA
                     DGGFPWGVFEILGELGQTFVGMGQVRRGTVLLGAVEALISGQGRFCRCCTIPFTLAGE
                     KTRAATRAQLGDAEFEKTWQEGRALSLEQAVEFARAEGPQLSEPTPSKESRAKALNTA
                     ANLARMQGDVTECRVNAEEALQLAREIGDRLGEVDALNNLAGIETNLERARNLYEECL
                     AIRKELGDRSAIAGATMNLGVIAMSLGDYETARTRYEESLATARDVGNKALVAWIIFN
                     LAGLATEQGDVGQARIFLEESLSMCEELGDRDSVCNARIMLANLAAEEGDYTTARSLY
                     EESLATARALGVKGYVAGILQGTGAVEVLQGETQAAIEHYEEVTALLREIDDEPGALI
                     FPGMAAGLRGEHKKALALHEEALALSATLGNPRFRIEALALVGSDFVNMGQARRGAVL
                     LGAADSLISALGTKLCFPVRIPCGRAQATARAQRGDSEFEKAWQEGRAMSMEEAIEFA
                     RSEAAPPTGPAPSTESRIKALNTASALARSQGDLPAARSLAEEALKLAKETDEREGEA
                     DALAILGGIASDLWYIKEACALYEESLAKWKELGDKAGIARTLMALGWATAKGGDRRR
                     ARSLYEESLAMWRELGDRAMVAWDIFNLGGVASEEGDFDRASALYEESRKLCEEIGDR
                     ESGCQLVACLGNDALEEGDFDRASDLFERSLATARELDVKQDIVLALMGLGNLASMRG
                     NSPSASAFFDKVAALEKEMGDQVFSVFFSGVAANHRGEYTTARTLLEEVLATFEKRGH
                     RKGMAETLGEVAEALVGMGQTRRGVMLLGSTQHTSEELGVLCRACSISFERAHERLRA
                     IARAQLGDGDFEKAWQQGRAMSMEKAVEFARAEFVLP"
     gene            complement(3081..5537)
                     /locus_tag="VHS06_08040"
     CDS             complement(3081..5537)
                     /locus_tag="VHS06_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HEX2988104.1"
                     /translation="MDLNYSNRYQTLRSRLQARPRKPATRLVAIAALALLIVMSLAAC
                     DPTALTGLPIESISPNSTVPSAPGSSGGSGAQTAPSAPSTAPSFGGAPSQSAPSTDAT
                     AAIKAVIEKANQEEVQAFASQNPSVMADTATSTYYQQMAQQYNSMSNSGITAIQLLNL
                     TWGNITQQDANTAQANTTETWQTTYSDGSTTQDTNANVYTLVLQNGSWKIQDDQQPNS
                     RNQQPGSGNQGNPTIPTNPGNPGTQGSGTPPFRGRPGVTPTPVVPTAPDSTNPFGNVP
                     SPSGPTSSAPSADTTAAIKAVIEKGNQEEVQAFASQNPSLMADTATSTYYQAMVQNYN
                     SMANAGVQSIQLLNLTWGNISLQDANTAQASTTETWQTTYSDGSTTQDTNANVYTLVL
                     QNGSWKIQDDQQPNTHTLQPQPGNQGNPNTTPSIPSAPTPSGPSSSAPSGNTTAAIKA
                     VIEKGNQEEVQAFASQNPSLMADTATSTYYQQMVQNYNSMANSGVQSIQLLNLTWGNI
                     TQQDANTAQANTTETWQTTYSDGSTTQDTNANVYTLVLQNGSWKIQDDQQPQSGNQGN
                     PTSPTIPNNPGNPGTGGVAPVPPSTSVGQSSNWAGYAATGGTFTAVSGNWTVPNVSAG
                     SGSTMSADATWVGIGGVNTTDLIQAGTQAIVQGGQVEYSAWWETLPQTPVTVPLDVNP
                     GDNINVSITQQSSGTWLVVIHNLTTAQSFQKTVTYRSSLSSAEWVEEAPTAARGTLPL
                     DAFGTVNFTQGVALENGQQVTIKQANGQPISMYGSSGIGRRGLYGQPSGRSGGPLAQP
                     SAIGADGSSFSVTRTNVALP"
     gene            complement(6211..6576)
                     /locus_tag="VHS06_08045"
     CDS             complement(6211..6576)
                     /locus_tag="VHS06_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988105.1"
                     /translation="MVATKTKSLLLIEDNGEYYLIPHDEIVSFRVSEDQKAELSKLFG
                     DEVTGYKAWAEGTVGPHMESTTGPHMEGTTGPHMEGTMGPHVEGTTGPHMESTMGPHM
                     ETIVGRPMALWIGGWPVKY"
     gene            complement(6755..7147)
                     /locus_tag="VHS06_08050"
     CDS             complement(6755..7147)
                     /locus_tag="VHS06_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988106.1"
                     /translation="MEDKATIEDEVLILGDQEGNLYVIPREVVESYRASAEQKAEIRE
                     LLEEEVTGFQMPSDFLLHSLVEQHEAQRLEEASHERMARTALMEEEEKEEPPGGTKVG
                     HPPVTGVGHPLTGLWRRLHLVQHAKPAR"
     gene            complement(7342..8058)
                     /locus_tag="VHS06_08055"
     CDS             complement(7342..8058)
                     /locus_tag="VHS06_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2988107.1"
                     /translation="MTLSTSPDIVAFEEIALFRGLTRGELLRIQPLLHRLHLPAETYL
                     FAREQPGEIAYVIVQGAIKIYMEQGDGGQVILSILGQGEVVGEMSLVDYLGRSASAMT
                     IEQCDLLWMDRTSLNDCLDAIPELNHNLVRMLTRRLRMADELIQSLATLDVHGRIARQ
                     LLMLSSEYGMPAPGSRPGVGAVLIPFRLTQNDLADLVGASRGRVNQALADFKEQGSIA
                     IDHEHRITVLDASALGRRCV"
     gene            complement(8107..8784)
                     /locus_tag="VHS06_08060"
     CDS             complement(8107..8784)
                     /locus_tag="VHS06_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988108.1"
                     /translation="MKTVEAPQSTAKLPTVKEMPAAEKFSKVIADARFTRDLVTPFVR
                     EHISEEAVSELRNTWRQGIQPIPKDAPPEEKYEKAYANTMWIGSSSLGFIREKLGEDG
                     MDEWVRTEAEDLKRRNAGPAVLLLSLIRAVAPEFAFRMMAKQMSYELQWLTPFDVTEL
                     TGSKVVYSIPRCKVLDYPNTEDVCQVGCQRVYPQWQAEQFKARMSYERVDHSCTCTVT
                     PLRKELK"
     gene            complement(8848..9978)
                     /locus_tag="VHS06_08065"
     CDS             complement(8848..9978)
                     /locus_tag="VHS06_08065"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX2988109.1"
                     /translation="MQSGELLRKFSGHTIGVVTSLFSPDGQYLLTLDFAGIMRLWQTR
                     ALNGLPQFIGHTAGVETAAISPDGKLVVTGGMDNVARLWNAETGEQVRVLQGHTSYIN
                     WVAISPDGKRLLTAGWDDNTARLWDMQTGKEIHRFDFAGRVLSVAISPDGRYAIMSVG
                     STLPVFDLLTYTQVYTLTTGSVQKDAAEGVTFSPDSKYALSAGMDSIARLWDMSTGKQ
                     VREFKGHAGGIYMAIFSPDGKYLLTSGFDKTARLWDVATGQEVRRFVGHSGEVLGVAF
                     SHDGKLVATTSGDNTARIWDVATGKELRSLQGHSAAVWWVAFSPDNKSVVTASDDGTA
                     RTWYVDYHDEMNYLCGKLLRDFTDAERAQFEIKDNTPTCPAK"
     gene            9973..>10372
                     /locus_tag="VHS06_08070"
     CDS             9973..>10372
                     /locus_tag="VHS06_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988110.1"
                     /translation="MHVPHEFGVGLVSSGDHRAVERQGHALDHFETGELAKQAAVSQR
                     AAARRLRVLLVGAVVFLLVAGILAVWALNQSSVAQANANEANTQRGVAQANFTHAEAQ
                     RLASEANTLILRNQNPETIALLALHSMNMEY"
BASE COUNT         1887 a         3013 c         3269 g         2203 t
ORIGIN      
        1 gcgcacctgg cagtagaggc gggtgactac gccggagccc gcgctgtata cgaggagagc
       61 ctggccacaa ccagagagct gggattcaat tacgaaatcg tccaggcgat tacgggcctc
      121 gcgaacctgg cgtcggtgga gggagatcat gtggctgccg ctcgccttta cgatgaggtc
      181 gcggaactgc accaggatct gggcgaggaa gtccggtggc ttcagttcag ggggatagca
      241 gccaatcacc gtggcgagca cgcagaggcc cgcacgctcc tgctgcaggc gcttggaatg
      301 ttcgcggatg gagggttccc gtggggggta tttgagatac ttggagagct ggggcaaacg
      361 ttcgtgggca tgggccaggt gcgaagaggg accgtgctac ttggggcagt ggaagccctg
      421 ataagcgggc aaggcaggtt ctgtcgatgc tgcacgatac ctttcacact ggccggcgag
      481 aagacaagag ccgctacccg cgcccagctt ggggacgcgg agttcgagaa gacctggcaa
      541 gagggccgtg cgttgagcct ggagcaggct gtcgagttcg cgcgggccga ggggccccag
      601 ctatccgagc ctactccatc gaaagagagc cgggctaagg ctctgaacac ggccgccaac
      661 ctggcaagga tgcagggcga tgtcaccgag tgtcgggtca acgccgagga agcactccag
      721 ttggccaggg agataggcga ccggctcggc gaggtcgatg ccctcaacaa tctggccggc
      781 attgagacca acctcgaacg tgcacgcaac ctgtatgagg agtgcctggc cataaggaag
      841 gaactcggcg ataggtcggc tatcgcaggt gcgaccatga acctcggcgt catcgctatg
      901 agcctgggcg actatgagac agcacgcacc cggtacgagg agagcctggc gacggcgagg
      961 gacgttggca acaaggcgct agtggcctgg attatcttca acctggccgg gttggcgacc
     1021 gagcagggag acgtcgggca agcacggatc ttcctggaag agagcctgag catgtgcgag
     1081 gagttgggcg atagagactc ggtgtgcaac gcgcgcatca tgcttgcaaa cctggctgcg
     1141 gaggagggtg actacaccac ggcgcggtct ctgtatgagg aaagcctggc gacggccagg
     1201 gcactcgggg tgaaagggta tgttgcgggg atactgcaag gcacgggcgc tgtggaggtg
     1261 ctgcagggag agactcaggc ggccattgag cattatgagg aggtgactgc actcctgagg
     1321 gagatcgacg acgagcctgg cgcgctcatc ttccctggaa tggcggccgg tctgcggggc
     1381 gagcacaaga aggccctggc cttgcacgag gaggcgcttg ctctctctgc cacgctaggt
     1441 aacccgagat tcaggattga ggcacttgcc ctggtgggca gcgacttcgt gaatatgggc
     1501 caggccagac ggggagccgt gctgctcgga gcggctgata gcttgataag cgccctgggg
     1561 acgaagttgt gcttcccagt caggatacct tgcgggcgcg cacaagccac ggcccgtgct
     1621 cagcggggcg attccgagtt cgagaaggcg tggcaggaag gccgggcgat gagcatggaa
     1681 gaggcgatcg agttcgcgcg gtccgaggcc gctccgccga ctggacctgc cccatcgaca
     1741 gagagcagaa taaaggcgct aaacacggca agcgcgttgg cgagatccca gggcgacctt
     1801 ccagcggctc gctctcttgc cgaggaagcg ctgaagctgg cgaaggagac cgatgagcga
     1861 gaaggtgaag ccgatgcgct ggccatcctt ggcggcatcg cttcggacct ttggtatatc
     1921 aaagaagctt gtgctctgta cgaggagagc ctggcaaaat ggaaagaact cggcgataag
     1981 gcggggatcg cacgaaccct gatggccctg ggctgggcaa ctgcgaaggg cggggatcgt
     2041 cgaagagcac gctccctcta cgaagaaagc ctcgcgatgt ggagagaact gggcgaccgg
     2101 gcgatggttg cctgggacat cttcaacctt ggaggagtgg cgtcggagga aggagacttc
     2161 gaccgggcaa gtgccctcta cgaggagagc cgaaaactgt gcgaagaaat tggtgacagg
     2221 gagagcggct gccaactcgt agcgtgcctg ggaaacgacg cccttgagga aggggacttc
     2281 gaccgggcaa gcgatctgtt cgaacgaagc ctggcgacgg ccagagagct ggatgtgaaa
     2341 caggatatcg tgttggctct catgggcctt gggaatctgg catccatgcg ggggaactcc
     2401 ccctcggctt ctgctttctt tgacaaggtg gcggcgctgg agaaggaaat gggtgaccag
     2461 gtattctcgg tctttttctc tggggttgcg gccaaccacc gcggcgagta cacaaccgcc
     2521 cgcactctgc tggaggaggt gctcgctact ttcgagaaga gagggcaccg aaaagggatg
     2581 gccgaaaccc tcggagaggt cgctgaagcg ctggtgggca tgggccagac tcgtaggggg
     2641 gtcatgctcc tgggaagcac gcaacacacg agcgaggagc tcggggtgct atgccgcgcg
     2701 tgcagcattt ccttcgagcg agcccacgag cgactaagag ccattgcccg ggcccaactt
     2761 ggggatgggg atttcgaaaa ggcatggcag caaggccggg caatgagcat ggagaaggcc
     2821 gtcgagttcg ccagagcgga gtttgttctt ccctaaacag gaaacccacg cgcaagagcc
     2881 tgattgaccc tgcacatggg tttactgcta ttcacttgtt gggctacgcc ggtcaggtta
     2941 ccctgctggc accggtgcgt cccttagtcc ctcggtgagc tgactgcctt tagagagcac
     3001 cacccttgcg gcgcccgagt gcatctcaac ctgacagtca actcaccgaa ccctatctgc
     3061 gagcctcagt tatctcacca ttatggcaga gccacgttgg tgcgggtaac cgagaagctc
     3121 gacccatcgg cgccgatcgc ggacggctga gcaagcgggc ctcctgaccg tccactcggc
     3181 tggccgtaca ggccccgccg gccgataccg gacgaaccgt acatgctgat cggctgcccg
     3241 ttagcctgct tgattgtcac ctgctggccg ttctcgagcg ccacgccctg ggtgaagttc
     3301 acggtgccga aggcgtccag gggcagcgtg ccgcgtgccg ccgtcggggc ttcctcaacc
     3361 cactcggccg aggagagcga ggagcggtac gtcacggtct tctggaaaga ctgcgcagtc
     3421 gtcaggttgt gtatgaccac cagccatgtg ccgctcgact gctgggtgat cgaaacgttt
     3481 atgttgtcgc ccgggttcac atcgagcggt acggtcaccg gggtttgcgg aagggtctcc
     3541 caccaggcgc tgtattcgac ctggccgccc tgaacgatag cctgtgtgcc cgcctggatc
     3601 aggtcggtcg tgtttactcc gccgatgcca acccaggtgg cgtcggccga catcgtactg
     3661 ccactgcccg cgctgacgtt cggtactgtc cagttgccgg aaacagcggt gaaggtgccg
     3721 cccgtagccg cataaccggc ccagttgctc gactggccga cgctggtcga cggcggaacg
     3781 ggagccacgc ccccggtacc cgggttgcca gggttattcg ggatagtcgg gctggtcggg
     3841 ttgccctggt tgccggattg aggctgttgg tcgtcctgga tcttccagct accgttctgg
     3901 agcaccaggg tgtagacatt ggcgttggtg tcctgggtgg tgctgccgtc cgagtaggtg
     3961 gtctgccacg tctcggtggt gttcgcctgc gcggtgttgg cgtcctgctg tgtgatatta
     4021 ccccacgtca ggttcagaag ctggatcgac tgcacacccg agttggccat gctgttgtag
     4081 ttctgaacca tctgctgata gtatgtagaa gtagcggtgt cggccatgag cgaggggttc
     4141 tgggaagcga atgcctggac ttcctcctgg ttgccttttt cgattactgc cttgatggca
     4201 gcggtggtat tgcccgaagg cgcgctcgag gatggtccgc tcggtgtcgg agcgctcggg
     4261 atgctgggcg tcgtgttcgg gttgccctgg ttgccgggct gaggctgcaa agtgtgggtg
     4321 ttcggctgct ggtcgtcctg gatcttccag ctaccgttct ggagcaccag ggtgtagaca
     4381 ttggcgttgg tgtcctgggt ggtgctgccg tccgagtagg tggtctgcca cgtctcggtg
     4441 gtgctcgcct gcgcggtgtt ggcgtcctgc aggctgatgt tgccccacgt caggttgaga
     4501 agctggatcg actgcacgcc cgcgttagcc atgctgttgt agttctggac catcgcctgg
     4561 tagtaggtag aagtagcggt gtcggccatg agcgaggggt tctgagaggc aaacgcctgg
     4621 acttcctcct ggttgccttt ctcgatcact gctttgatgg cagcggtggt gtcagccgac
     4681 ggtgcgctcg acgtgggtcc actcggagac ggtacattgc caaagggatt ggtggagtca
     4741 ggcgcggtag ggaccacagg tgtaggagtt acgcctggcc ttccacggaa cggcggggtg
     4801 cccgagccct gtgtgcccgg gttgccgggg ttcgtcggga tcgtcgggtt gccctggttg
     4861 ccggacccag gctgctggtt gcgactattc ggctgctggt cgtcctggat cttccagcta
     4921 ccgttctgga gcaccagggt gtagacgtta gcattggtgt cctgggtggt gctgccgtcc
     4981 gagtaggtgg tctgccacgt ctcggtggtg ttcgcctgcg cggtgttggc gtcctgctgt
     5041 gtgatattac cccaggtaag gttcagaagc tggatcgccg tgatacccga attggacatg
     5101 ctattgtact gttgtgccat ctgctggtaa taagtagaag tagcggtgtc ggccataacc
     5161 gaggggttct gggaagcgaa tgcctggact tcctcctggt tagccttctc aattactgct
     5221 ttgatggcag cagtggcgtc agtcgagggt gcgctctgag acggagcacc gccaaaagac
     5281 ggggcagtgc tgggcgcact gggagcagtc tgggcaccgg aaccaccgga ggagccaggg
     5341 gcgctgggaa cggtggagtt gggcgaaatg gactcgatgg gaagccctgt cagcgccgtg
     5401 gggtcacaag ccgcgaggct catgacgatg agcagtgcca gcgccgcaat agctaccagc
     5461 ctggtggctg gtttccgcgg gcgtgcctgc aacctgctcc ttagtgtttg gtatcgatta
     5521 ctgtagttga gatccatgtc tttctttctt cctcttgttt tcgctctctg tggcaatcac
     5581 gaagagctta ttgttgtcgt agtacacatg cttcgggtgt aactacagtg tcttgcagcg
     5641 tttccatgcc gatttccgcg gagaggaacc tggtccggtc aattggtcgc cctgggcgac
     5701 gccggcgctt gtgagtacct tacgggggaa atggatccgg ctcgcgctcg cctcttcgag
     5761 gcttcggatc acgatctgcg gagcggagca gaacccgtac gtgcgccatg agttcccatg
     5821 atcaggttgc caatctacaa agtcatctac gtcctatcga ggctctccgg tacatcttct
     5881 tactgctgct tccgggcagg ataattggca ctgctggctc gctgcgcagg agccgcacca
     5941 gtttggtgaa cggcagatct ttccccagta ctgtttttga cgccttatga tagatcattt
     6001 gcgatcctcg tcgctttgtt cgcagcctga gccaagctta tatctgtgcc ctctattatc
     6061 agcatactat gcaatgctta aaccaacatg tgaaaagagt taagttttca ttagacgctt
     6121 ccgggtgcca aaagggccta tcctcacgga taggcccctg cgccggaccg ggtgttaacc
     6181 ggcgcccgga gagcaagccg gggtgcgtga tcagtacttg accggccaac caccaatcca
     6241 gagagccatg ggccgcccga ctatggtttc catatgcggc cccatcgtgc tctccatgtg
     6301 cggtccggtc gtgccctcca catgtggccc catcgtgccc tccatatgcg gtccggtcgt
     6361 gccctccatg tgcggcccgg tcgtgctctc catatgcggt ccaaccgtgc cctcggccca
     6421 ggccttatac ccggttacct catcgccgaa gagcttgctc agttctgcct tctgatcttc
     6481 ggaaacgcgg aagctgacta tctcatcatg cgggatcaga tagtactcgc cgttatcctc
     6541 gatcagcaac aacgatttag ttttggtcgc taccatctcg gttctccttt cctttcgtgc
     6601 tacattgagc acaacaaccc tccacgctcc gaccggcttg caccgttcga ccgtgccgag
     6661 cccacccacc ggcgtccctt ccgggcgcca ggttcactat ataaggctgc agagccgcgt
     6721 ttgcagacct gcctctctca cattccacac gccgctacct ggcaggcttt gcgtgctgaa
     6781 ccagatgaag ccgtcgccac agcccggtca gcgggtgccc gactccggtg acaggcgggt
     6841 gcccgacttt ggtgccgccg ggcggctcct ctttctcttc ctcttccatg agcgcggttc
     6901 tcgccatgcg ctcatgcgaa gcttcctcga gtcgctgggc ctcgtgctgc tctaccaggc
     6961 tgtgcaagag gaaatcgctc ggcatctgga agccggtaac ctcctcctcg agcagttccc
     7021 ggatttcggc cttctgctcg gcagacgctc tgtagctttc gacaacctca cgcgggatga
     7081 cgtagaggtt gccttcctgg tcgcccagta tgagcacttc atcttcgatg gttgccttgt
     7141 cttccatcgc cgtctccttt cctcagaatt gcgcttgaga tatcagcttc caggttgctg
     7201 cctcaagcgt aggctgcaac ctgggaaacg tccccctcct atttcctcct tctcatagta
     7261 gcgtgcatgt cctctcgccg cgagcgaatc tatgacataa cgcctgtata tttggttaca
     7321 gtcgtcgtcc ccggctgccc tctagacgca acgcctgccg agcgcactcg catcaagcac
     7381 agtgatacgg tgttcatggt ctatcgctat ggaaccctgc tccttgaagt cggcaagggc
     7441 ctggttcacc cgaccgcgcg acgcgccaac caggtcggcc aggtcgttct gtgtcagccg
     7501 gaagggtatg aggactgcgc cgacccccgg ccgagaacct ggcgccggca taccgtactc
     7561 tgaggaaagc atgagtaact ggcgagcaat ccggccgtga acgtcgaggg tcgcgagcga
     7621 ctggataagt tcgtcggcca tgcgcaggcg acgggttagc atgcgaacca ggttgtggtt
     7681 gagctcggga atcgcgtcca ggcagtcgtt gaggcttgtg cggtccatcc acaggagatc
     7741 acactgctcg atcgtcatcg cgctggcgga acgccccagg tagtcgacca ggctcatctc
     7801 tcccacgact tccccctggc ccaggatgga gaggatcacc tggcccccat cgccctgttc
     7861 catgtagatc ttgatcgcgc cctggacgat gacataagca atctcgcccg gctgctccct
     7921 ggcgaagaga taggtttcgg ccgggagatg gagcctgtgc agaagaggtt ggatcctcag
     7981 gagctcccct cgagtaaggc ctcgaaacag cgcgatctcc tcgaacgcta cgatatcggg
     8041 acttgtggat aaagtcatac tatgcactcc tttccggcac ctcagtttcc aatcgcgttg
     8101 cattgatcat ttgagttcct tgcgcaaagg agtgaccgtg cacgtgcagc tgtggtccac
     8161 acgctcgtac gacatccgcg ccttgaactg ctcggcctgc cactgcgggt agaccctctg
     8221 gcagcccacc tggcaaacat cttcggtatt cggatagtcg agtaccttgc acctcgggat
     8281 ggagtagacg actttgctcc cggtcagttc ggtcacatca aaaggcgtga gccactgtag
     8341 ctcgtacgac atctgcttgg ccatcatcct gaacgcgaac tccggcgcca ccgccctgat
     8401 gagggacagg agtagcactg cggggcctgc attccggcgc ttcaggtcct cggcctctgt
     8461 gcggacccat tcgtccatgc catcctcgcc cagcttctct cgtatgaagc cgaggctgga
     8521 actcccaatc cacatggtgt tggcataggc cttctcgtat ttctcttcgg gcggagcgtc
     8581 cttcggtatt ggttggatcc cctgccgcca ggtgttcctc aactccgaca cggcctcctc
     8641 ggagatatgc tcgcgcacga atggcgtcac caggtctctc gtgaacctgg cgtcagcaat
     8701 cactttggag aatttctccg ctgcgggcat ctccttgacg gtgggcaact tcgccgtgct
     8761 ctgcggtgcc tctaccgttt tcatctcttt gcccctttca gccgaccggc gccccaactg
     8821 gtacgtcgcc tgtccaacta cctcttctca ctttgccggg caggtgggtg tgttgtcctt
     8881 gatctcgaac tgcgcccgct cggcgtcggt gaagtcgcgc agcagcttgc cgcacaggta
     8941 gttcatctcg tcgtggtagt ccacgtacca cgttcgggcc gtgccatcgt cgctggcggt
     9001 taccacactc ttgttgtcgg gcgagaatgc cacccaccag accgcagcgc tgtgtccctg
     9061 caggctccta agctccttgc ctgtcgccac gtcccatatc cgggctgtgt tgtcgccgct
     9121 cgtagttgcc accaacttgc cgtcgtgcga gaaagccact ccaagcacct cgccagaatg
     9181 accaacgaag cggcgcacct cctgccctgt cgcaacgtcc cacagccggg cagtcttgtc
     9241 gaagccgctc gtcaacaggt acttgccgtc gggcgagaag attgccatgt aaatgccacc
     9301 ggcatgccct ttgaactcgc gtacctgttt acccgtgctc atgtcccaca atcgtgctat
     9361 cgaatccatg cctgcgctga gggcgtactt gctatcaggt gagaaagtca ccccctctgc
     9421 cgcgtctttc tggacgctac ccgtagtgag cgtgtagacc tgggtgtagg tcaggaggtc
     9481 gaacaccggt agcgttgagc ccacgctcat gattgcgtac ctgccatcag gagagatagc
     9541 gacgcttagc accctaccgg cgaaatcgaa acggtgtatc tccttgcctg tttgcatgtc
     9601 ccacagcctg gcagtgttgt catcccatcc tgctgtgagg aggcgtttgc cgtcgggcga
     9661 gatggctacc cagttgatat agcttgtgtg gccttgaagt accctcacct gctctccggt
     9721 ctcggcgttc cacaatcgtg cgacgttgtc cattccccct gttaccacca gtttgccgtc
     9781 gggcgagatg gccgctgttt ccacgcctgc tgtatggccg ataaactgag gcaggccgtt
     9841 gagggctctg gtttgccata ggcgcatgat cccagcgaag tcaagggtga gcaggtactg
     9901 cccatcaggt gagaacaggc tggtcaccac cccgattgtg tggcctgaga acttgcgaag
     9961 gagttcaccc gattgcacgt cccacacgag ttcggtgttg ggctggtctc cagcggcgat
    10021 catcgtgccg tcgagcgaca gggccatgct ctggaccatt ttgagaccgg tgaactggcg
    10081 aagcaggctg cggtctcgca gcgcgccgcc gcgcgcaggc tgcgagtgct gctggtaggg
    10141 gctgtggtct tcctgttagt ggccgggatc ctggcagttt gggcactcaa ccagagtagc
    10201 gtggcccagg ccaatgctaa cgaggcaaac actcagcgcg gcgtggcgca ggccaacttc
    10261 acccatgcgg aagctcagag gttggcctcc gaggcaaata cgcttatcct gaggaaccag
    10321 aaccccgaga caatcgccct actcgccttg cactcgatga acatggaata ta
//
LOCUS       DATALS010000360         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_222829, whole genome shotgun sequence.
ACCESSION   DATALS010000360 DATALS010000000
VERSION     DATALS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222829"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1244
                     /locus_tag="VHS06_08075"
     CDS             33..1244
                     /locus_tag="VHS06_08075"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX2988111.1"
                     /translation="MDCKADFPILARQINGKPLTYLDSTATAQKPQVVIDSMVDYYTR
                     YNANIHRGIYTMAEEATAAYEDARKRLGRFIGAQSAKEVIFTRNTTEAINLVAQTWGR
                     QNLKHGDAVLLTEMEHHSNLVPWFILAEQIGFSIRYIPITPDGQLDLSVLPQVLTERT
                     KLVSLVHVSNVLGTINPVRKIADEAHKVGALCLVDGAQSVPHMPVNVQSLGCDFLALS
                     GHKMAGPTGSGALWGRKHLLEDMPPFMGGGEMIRNVTYEGARWNELPWKYEAGTPAIA
                     EGIGLGAAVDYLQGLGMDKVRAHEETLTAYAMERLHAMRGVTIYGPAAEKRGGVIAFN
                     LEKIHPHDMAAILDQDGIAIRAGHHCAQPLHHKLGIPASARASFYVYNDASDVDRLIA
                     SLERAQAFFGV"
     gene            1246..1647
                     /locus_tag="VHS06_08080"
     CDS             1246..1647
                     /locus_tag="VHS06_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX2988112.1"
                     /translation="MDIYREVILDHYQNPRHHGELADADVSYEDNNPLCGDRIRMQLK
                     VDKDDRIVDVAFDGQGCAISQASASMLTEEILGKKLPDVLALGRQDVLDNLGIELTPI
                     RLKCALLSLGVLKAGIYQYKGQKPVDEDEEL"
     gene            1704..2015
                     /locus_tag="VHS06_08085"
     CDS             1704..2015
                     /locus_tag="VHS06_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX2988113.1"
                     /translation="MSKYIAIAKTSEIQPGQIKYVEIEDYRLALCNLNGTFYCFENTC
                     THDGGPLNQGELMGDVVECPRHGGRFNVTTGKAVRMPAVAPIEVYPLIVEGDEIKVDL
                     E"
     gene            2012..2509
                     /locus_tag="VHS06_08090"
     CDS             2012..2509
                     /locus_tag="VHS06_08090"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2988114.1"
                     /translation="MINDSMLQSGQEAPAFSLTGADGQTYDTAARGNGPLVLFFFKVG
                     CPTCALSFPYMEKLYQTYRDAGLMLWGIAQDPKDKAVQFAQQGGATFPILLDDGYPVS
                     NQFGIHTVPSFYLISRADSVEFERYSFMKDELNLLAGLVAEQVKRPASTLAPADDGKP
                     AFKPG"
     gene            2574..2849
                     /locus_tag="VHS06_08095"
     CDS             2574..2849
                     /locus_tag="VHS06_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX2988115.1"
                     /translation="MTVRIPAALRSVTHGEAVVSEAAPSMQVLLDRLEERYPGFRDRL
                     CQPDGDLKRFINIFVNGQEIRRLQGLETPLHDADEVVIIPAMAGGSL"
     gene            2860..3225
                     /locus_tag="VHS06_08100"
     CDS             2860..3225
                     /locus_tag="VHS06_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2988116.1"
                     /translation="MNVPFGRSQGSPSPAPQQSYIDLTVEETKRRLAEGDYDLVDVRE
                     PWEYQLGHLPGARLVPLNTLLIQARQQLTRDNVIFVCAHGNRSRVASEMAVAVGLKNV
                     YNMAGGTSEWVNKGYPVEK"
     gene            complement(3264..>3514)
                     /locus_tag="VHS06_08105"
     CDS             complement(3264..>3514)
                     /locus_tag="VHS06_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2988117.1"
                     /translation="DLTVEETKRRLAEGDYDLVDVREPWEYQLGHLPGARLVPLNTLL
                     IQAQRNRESERGTELGGGAGGMRGRASQLSPAWDRRNT"
BASE COUNT          666 a         1126 c         1118 g          604 t
ORIGIN      
        1 aggcaaaggt aaccgaacag gccgagcagc ctctggactg caaggcggac ttcccgatcc
       61 tggcccggca gatcaacggc aagcccctga cctacctgga ctccacggcg acggcgcaaa
      121 agccgcaggt ggtcatcgac agcatggtgg actactacac ccgctacaac gccaacattc
      181 accgcggcat ctacaccatg gccgaagagg cgacggccgc ctatgaggac gcgcgcaagc
      241 gcctgggccg cttcatcggc gcgcagagtg ccaaggaggt gatcttcacc cgcaacacca
      301 ccgaggccat caacctggtg gcgcagacct gggggcgcca gaacctgaag cacggcgacg
      361 cagtgctgct caccgagatg gagcaccact ccaacctggt gccctggttc atcctggccg
      421 agcagatcgg cttcagcatc cgctacatcc ccatcacgcc cgatggccag ctcgacctct
      481 cggtgctgcc gcaggtgctg accgagcgca ccaagctggt gagcctggtg cacgtctcca
      541 acgtgctggg caccatcaac ccggtgcgca agattgccga cgaggcgcat aaggtggggg
      601 cgctgtgtct ggtggacggc gcgcagagcg tgccgcacat gccggtgaac gtgcagtcgc
      661 tgggctgcga cttcctggcc ctgtcgggcc acaagatggc ggggcccacg ggcagcggcg
      721 ccttgtgggg ccgcaagcac ctgctggaag acatgcctcc cttcatgggc ggcggcgaga
      781 tgatccgcaa cgtcacctac gaaggcgcgc gctggaacga gctgccgtgg aagtacgaag
      841 ccggcacgcc cgccatcgcc gaaggcatcg gcctgggcgc cgccgtggac tatctgcaag
      901 gcctgggcat ggataaagtg cgggcccacg aggagacctt gactgcctac gccatggagc
      961 gcctgcatgc catgcgcggc gtcaccatct acgggcctgc ggcggagaag cgcggcgggg
     1021 tgattgcctt caacctggag aagattcacc cgcacgacat ggccgccatt ctggaccagg
     1081 acggcattgc cattcgcgcc ggccatcact gcgcgcagcc tttgcaccat aagcttggca
     1141 ttcccgcctc ggcgcgggcc agcttctacg tctacaacga tgccagcgac gtagaccggc
     1201 tcatcgcctc cctggagcgg gcccaggcat ttttcggagt gtaacgtgga catttatcgt
     1261 gaggtcatcc tggatcatta ccagaaccca cggcaccacg gcgagctggc ggacgccgac
     1321 gtctcgtacg aggacaataa ccccctttgc ggcgaccgca tccgcatgca gctcaaggtg
     1381 gacaaggacg accgcatcgt agacgtcgct tttgacggcc agggctgcgc catcagccag
     1441 gcctcggctt ccatgctgac ggaggagatc ctgggcaaga agctgccgga cgtgctggcg
     1501 ctgggccgcc aggacgtgct ggataacctg ggcatcgagc tgacgcccat ccgcctcaaa
     1561 tgtgctctgc tgtccctggg tgtgctgaag gccggcatct accagtacaa gggccagaag
     1621 cccgtcgacg aggacgagga gctatagcaa accgggcgcc ggggcccggc cgtattattg
     1681 ttgtgaggag tcgttagagt cgcatgagta agtacatcgc cattgccaag acatcggaga
     1741 tccagcccgg acagatcaag tacgtggaga ttgaggacta tcgcctggcc ctgtgcaacc
     1801 tgaacgggac gttctactgc ttcgagaaca cctgcaccca cgatggcggc ccgctcaacc
     1861 agggcgagct gatgggcgac gtggtagagt gcccccggca cggcgggcgc ttcaatgtga
     1921 ccaccggtaa ggccgtgcgt atgccggcgg tggcgccgat cgaggtctac cccctcattg
     1981 tcgagggtga tgagatcaag gtggatctgg agtgataaac gatagtatgc tacagtccgg
     2041 ccaggaggcc cctgctttca gtctcacggg cgccgatggc cagacctatg atacggctgc
     2101 ccggggcaac ggcccgctgg tgcttttctt cttcaaagta ggctgcccaa cctgcgccct
     2161 gtccttcccg tatatggaga agctgtacca gacctaccgg gatgcggggc tgatgctgtg
     2221 gggtattgcg caggacccta aagataaggc tgtgcagttc gcgcagcagg gcggggcgac
     2281 cttccccatc ctgctggacg acggctaccc ggtgtccaac cagttcggca tccataccgt
     2341 gcccagcttc tacttgatca gccgggcgga cagcgtcgag ttcgaacgct acagctttat
     2401 gaaggacgag ctgaacctgc tggccggcct ggtggccgag caggtgaagc ggccggccag
     2461 cacgctggcg ccggccgatg acggcaagcc cgccttcaag ccgggctgag ggtcccgcaa
     2521 cgcccggggc gggcggtaaa gtattttctt ctagaacgga tacactttct gctatgactg
     2581 tacgtattcc cgctgcactg cgcagcgtca cccacggcga ggctgtggtg agtgaggcgg
     2641 cgcccagcat gcaggtgctc ctcgaccgcc tggaggagcg ttaccccggc ttccgcgacc
     2701 gcttgtgcca gccggacggt gacctgaagc gctttatcaa catctttgtc aacggccagg
     2761 aaatccggcg cctgcagggg ctggagacgc cgctgcacga tgccgacgaa gtggtgatca
     2821 tcccggctat ggccgggggc tcactctaag gattatgtga tgaatgtccc gtttggacgg
     2881 tcgcagggct cgccctcgcc tgcaccccag cagagctaca ttgatctgac cgtagaagag
     2941 accaagcgtc gcctggccga gggcgactat gacctggtgg acgtgcgcga gccgtgggag
     3001 taccagcttg gccacctgcc cggcgcgcgg ctggtgccgc tcaacacctt gctcatccag
     3061 gcccgccagc agctcacccg cgacaatgtc atcttcgtct gcgcccacgg caaccgcagc
     3121 cgcgtggcct ccgagatggc ggtggctgtc ggcctgaaga acgtgtacaa tatggccggc
     3181 ggcaccagcg agtgggtgaa caagggctac ccggtcgaga agtagcgcga ccctagaact
     3241 gaagcttagc atgacgactg atattatgtg ttgcgcctgt cccaggcggg gctgagctgg
     3301 gaggctcgtc cgcgcattcc ccccgcccct ccccccagct ccgtcccgcg ttccgattct
     3361 ctattccttt gtgcctggat aagcaaggtg ttgagcggca ccagccgcgc gccgggcagg
     3421 tggccaagct ggtactccca cggctcgcgc acgtccacca ggtcatagtc gccctcggcc
     3481 aggcgacgct tggtctcttc tacggtcaga tcaa
//
LOCUS       DATALS010000361         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_39407,
            whole genome shotgun sequence.
ACCESSION   DATALS010000361 DATALS010000000
VERSION     DATALS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39407"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VHS06_08110"
     CDS             <1..1384
                     /locus_tag="VHS06_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988118.1"
                     /translation="RTVTRDGMNMNAGMNAMMNETLNVSGALLVKIFGRERDENRRFQ
                     ERAAGVRDVGIRQAVIGRWFFMGLSLISSVGTALVFWWGGMLVLHGEFTVGTIVAFGA
                     YLTQLYGPLTALTNARVEFATSMVSFERVFEVLDLPQEIFDRPGAVTLDRVAGDVRFE
                     NVSFSYKDGDGVPGVFGLSEVQRWHWGGDAVPVTKSPRHKGSGDRANGDSHGGNGRDG
                     VEEATSGSLRNWSLRDVSFEIKPGQLAALVGPSGAGKTTITYLLPRLYDPTAGRILID
                     GVDIRDITLESLSSQIGMVTQESYLFHDTIRANLLYARPNATQAQLEDACRAANIHDL
                     IVGLSDGYDTVVGERGYRLSGGEKQRIAIARVILKDPRILVLDEATSHLDSHSEALIQ
                     AALERVMVGRTSLVIAHRLSTILAADLILVIDEGRLVEQGTHHELLARGGLYADLYET
                     QFRERAEESV"
     gene            complement(1516..2922)
                     /locus_tag="VHS06_08115"
     CDS             complement(1516..2922)
                     /locus_tag="VHS06_08115"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX2988119.1"
                     /translation="MDQSILGPHTGLTVDLYHPDSAYVSWRSRRNGLATFDLFSRSAP
                     FGGAYMLVAGLELALEFVQSFRFTDEDLRYLSQIRDYDHAFLDHLRNLRFTGEILAMP
                     EGSIAFPNEPLLRVTAPFQEALLLESGLLHMVSLSTLIATKASRLVHAAKGRPVADFA
                     LRRAQSPFVVTRSAYIGGCSSTSFLAAAYEYRLRASGTIPHALVQLYDSEREGFAAVA
                     ESFNRYNLLLDTYQVHDAIHTAVEVAREYQDRMGHTLVAVRLDSGDLGADARYVRGVL
                     DDAHMPEVRILASGDLDEFRIEELMQQNAPIDGFGVGTSLGVGGASLKHNAPGCSLGA
                     VYKMSYYEDIEAVDRPKIKLAGEKSTWPGKKEVYRIGSFERDVIQLAEEPRPDDGARM
                     LKPVVQDGRLLPGALPPLSEIWELAQRNLQQLPEEYAALTATRRYPVDRSTGLVALRK
                     QAIQRYGGEERPEDWEAM"
     gene            complement(2886..3170)
                     /locus_tag="VHS06_08120"
     CDS             complement(2886..3170)
                     /locus_tag="VHS06_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HEX2988120.1"
                     /translation="MPQEVAIGDVTVQIVLIIGLILLNSFFAAAEIAIVSMRRSRIRH
                     LAEEERDPRAARVARLVEEPGRFLATIQIGTTLGSEGSTWIKAFLVHTQG"
BASE COUNT          633 a          982 c         1111 g          642 t
ORIGIN      
        1 gcgcaccgtg actcgcgatg gcatgaacat gaacgccggc atgaacgcga tgatgaacga
       61 gacactgaac gtcagcggtg cgctgctggt gaagatcttc ggccgggagc gagacgagaa
      121 tcggcggttc caggagcggg ccgcgggcgt gcgggatgtg ggtatccgcc aggccgtgat
      181 aggccggtgg ttcttcatgg ggctgagcct gattagctct gttgggacgg cgctcgtgtt
      241 ctggtggggg ggcatgctgg tgctgcacgg cgagttcacc gtgggaacta tcgtggcctt
      301 cggagcctac ctgacccagt tgtatggccc gctgacggcc ctgaccaacg cgcgggtgga
      361 gttcgccacc tccatggtca gcttcgagcg ggtcttcgag gtgctggatt tgccccagga
      421 aatcttcgat cgaccgggcg cggtgaccct cgatcgcgtg gcgggtgacg ttcgcttcga
      481 gaatgtctcc ttcagttaca aagatgggga cggagttcca ggcgtgttcg gcctatcgga
      541 ggtgcagcgg tggcattggg gaggcgacgc tgtgccggtc acgaagagtc cgcgacacaa
      601 agggagtggt gatcgggcga acggtgattc tcatgggggg aatgggcgag acggcgtaga
      661 ggaggctact tccggctcgc ttcgaaattg gtcactgcga gacgtgagct tcgagatcaa
      721 gccggggcag ctagccgcgc tggtgggccc cagcggcgcc gggaagacga ccatcaccta
      781 tctattgcct cggctgtacg atcccacggc cgggcggatc ctgatcgacg gtgttgatat
      841 ccgtgacata actctggagt cgctttcctc gcagatcggg atggtcacgc aggagagcta
      901 tctcttccac gataccattc gggccaatct gctgtatgct cgccccaacg ccacgcaggc
      961 gcagttggag gatgcctgtc gggccgccaa catccacgac ttgatcgtcg gattgtccga
     1021 tggatacgac acggtggtcg gcgagcgcgg ctaccggctt tcgggcggcg agaagcagcg
     1081 gatcgccatc gcccgggtga tcctgaagga tccaaggata ctggtcttgg acgaggcaac
     1141 ctcccacctg gactcgcact ctgaggcgct gatccaggct gccctggaga gagtgatggt
     1201 tggccgcacc agtctggtca tcgcccatcg gctcagcacc atactggcgg cggacctgat
     1261 cctggtcatc gacgagggtc gcctggtgga acaggggact catcacgagt tgctggcccg
     1321 aggcgggctg tatgccgatc tgtacgaaac gcagtttcga gagagggcgg aggagagtgt
     1381 atagacttgc ataccggatg atggccgacc tggggtaagt ggtggggccg atgttgttag
     1441 ggcggttggc ggacgtctcg cgaaacgaga cgtcgaacat tgccacgggc tacccgtcgg
     1501 ggacgagccc cgacgctaca tggcttccca gtcctcgggt cgctcctccc cgccgtagcg
     1561 ctggatggcc tgctttcgaa gcgccacaag ccccgtactc cgatcgacag ggtagcgtct
     1621 ggtggccgtc agcgcggcgt actcctccgg caattgctgt agattgcgct gggccagttc
     1681 ccagatctcg gagagtgggg gcagagcacc cgggagaagc cgtccatcct gcacgaccgg
     1741 cttcagcatc ctggcgccgt cgtccggcct gggctcctcc gccagttgaa ttacgtctcg
     1801 ctcgaagctg cctatcctgt agacctcctt cttgcccggc catgtggact tctccccggc
     1861 aagcttgatc tttggcctat cgacagcctc tatgtcctcg tagtaagaca tcttgtagac
     1921 cgctcccagg gagcaccccg gggcgttgtg tttcagcgag gctccgccga cacccaggct
     1981 ggtacctacc ccaaacccat cgatcggagc gttctgttgc atcaactcct cgattcggaa
     2041 ctcgtccagg tcgccgctgg cgaggatacg aacctccggc atatgggcgt cgtccagcac
     2101 cccgcggacg tagcgtgcgt cggcgcccag atccccactg tccagccgaa cggccacgag
     2161 ggtgtgcccc atgcgatcct ggtactccct ggcgacctcc accgccgtgt ggatggcatc
     2221 gtgaacctgg taggtgtcca gcagcaggtt gtagcggttg aaactctcgg ccactgccgc
     2281 gaacccctcc cgctcgctgt cgtagagttg aaccagtgcg tggggaatgg tgccgctggc
     2341 gcggaggcgg tactcgtagg cggcagccag gaaggaggtg ctggagcagc cgccgatgta
     2401 ggcggatcgg gtaacgacga agggcgattg cgctcggcgc aacgcgaagt ccgcgacggg
     2461 tctacccttt gcggcatgaa ccaggcgcga cgctttggtc gcgatgaggg tggagaggct
     2521 gaccatgtgc agaagccccg actcgaggag cagtgcctcc tggaacgggg cggtgacgcg
     2581 gagaagcggc tcgttgggga aggcgatgga cccttccggc atggccagga tctcccccgt
     2641 gaagcgcaga ttccggaggt gatccaggaa cgcatggtcg tagtcgcgga tttgactcag
     2701 gtaccgcaga tcttcgtcgg tgaaccggaa cgactgcacg aactccaggg ccaactcgag
     2761 cccagccacc agcatgtaag cgccgccgaa gggtgcggac cgggaaaaga ggtcgaaggt
     2821 agccagaccg ttccttcggc tgcgccagct gacgtaggca gaatcgggat ggtacaggtc
     2881 caccgtcagc cctgtgtgtg gaccaagaat gctttgatcc aagtcgatcc ctccgagcct
     2941 agggtggtgc caatctggat cgtcgccagg aagcgacccg gttcttccac cagtcgggca
     3001 actctggccg ccctcgggtc tcgctcctcc tccgccagat ggcggatacg cgaacggcgc
     3061 atggagacta tggcgatctc agccgccgcg aagaaggagt tgagcaggat aagaccaata
     3121 atcaggacta tctggactgt gacatcaccg atggcaactt cctgcggcat agaccgctat
     3181 ccacccccaa gaaaaggagc aatttacgag gtacaatggg attgtacact attgtcggca
     3241 tctcttgact cagtcccaac gcggacatac agggccttta actttgggcc acagtgagag
     3301 tgatgcgtgc tgtcatgctg agcgaggcat ctcctacagt aaacggagag atccttcgct
     3361 atcgttca
//
LOCUS       DATALS010000362         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_288461, whole genome shotgun sequence.
ACCESSION   DATALS010000362 DATALS010000000
VERSION     DATALS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288461"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VHS06_08125"
     CDS             <1..403
                     /locus_tag="VHS06_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2988121.1"
                     /translation="WGPQPLAAVEDRMIPGPVGEIHLRVYTPEGKGPFPLLLYFHGGG
                     WVVGSVNTHDGVCRQLSLESGCMLVSVDYRLAPENKFPAALMDAQAALEWVFANAREL
                     NADPTRVAIGGDSSGGNIAAAVNRSSGYKFR"
     gene            430..603
                     /locus_tag="VHS06_08130"
     CDS             430..603
                     /locus_tag="VHS06_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2988122.1"
                     /translation="MAYKITDDCISCGACESACPTGAISQGDDKYVIDAAKCTEDGEC
                     AAVCPVDAPVKDA"
     gene            760..2580
                     /locus_tag="VHS06_08135"
     CDS             760..2580
                     /locus_tag="VHS06_08135"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX2988123.1"
                     /translation="MESPFLKRLRRGAILADGAMGTMLYSKGVSYERCFEEVTLSDPA
                     LVEEIHRAYLSAGAELIETNSFGANRFKLADYGLENRVREINLKAAKLARSTREIAGV
                     ECFVAGSVGPLGRPLAPVGQIRQAAASAAFQEQIGALLEGGVDLILLETFPDLEELAL
                     ALQVAHDLCDLPVVAQATFAEDGLTASGSTPEEVVRRLRDMGAAVAGVNCSLGPQQML
                     EIVQRMIAEGGVPISAMPNAGLPAMVGGRLIYRSTPPYMAEYALKYVHEGASMVGGCC
                     GTTPAHIAAMRTVLAELRPAEPLPPSCTLILDESPTPPAVGEPSPLKAKLGKQFVVSV
                     EMDPPKGANPTKLLNAASVLREVGVDAVNIADSPMARVRMSAMAMGFLVKQQLGLETI
                     IHFTTRDRNLMAIQSELIGAHAIGIRAILALTGDPPRLGDQPGTTAVFDVDSIGLIRI
                     IRRLNEGQDWRGNSIGEPTNFLVGCALNPNADDLELELDRFRQKLEAGVDFVLTQPVY
                     DMETLLRVLDRVEGVEAPILLGVLPLQSSRHTEYLHNEVPGITVPDPIRERMRQAGEH
                     GMDEGVTIAREFLREARSYVQGAYLMPSFGRFEQCAEILR"
BASE COUNT          502 a          814 c          924 g          528 t
ORIGIN      
        1 ctggggccct cagccgctgg cagccgtgga ggaccggatg atacccggtc ccgtgggaga
       61 gatacaccta cgtgtctaca ctccagaagg aaaggggcca ttcccgctcc tcctgtactt
      121 ccatgggggc ggctgggtag ttggcagcgt caacacccac gacggcgtct gccgccaatt
      181 gtcgctggag tccggctgta tgctggtctc cgtagactac cgattggccc ccgagaacaa
      241 gttcccggct gccctcatgg acgctcaggc cgccctagag tgggtgttcg ccaacgcccg
      301 cgaactgaat gccgacccca cacgagtcgc tatcggcggt gatagttccg gcggcaacat
      361 agcggcggcc gtgaacagat cgagcgggta taagtttcgg tagagagtac aaagaaggag
      421 ttgcgtagga tggcctacaa gatcaccgat gattgcatca gctgcggggc ctgcgagtcg
      481 gcttgcccga ctggggcaat ttcccagggc gacgacaagt acgtgatcga cgctgccaag
      541 tgcaccgagg acggcgagtg cgcggccgtg tgccccgtgg acgccccggt gaaggacgcc
      601 tagttcgggc ggtacccaca cctgtgggcc atgtgcccac ataccggtcg cggcagggac
      661 gcgtacgact taacagtgtc tctgagctct gcgacgagta ggctgccggg gaagctgaag
      721 agaaagcttc cccggttgct tttgtctgga gtgtttacag tggagagtcc atttctgaaa
      781 cggcttcgtc gcggagctat cctggcagac ggggcgatgg ggaccatgct ctactcgaag
      841 ggcgtttcct acgagcgctg cttcgaggag gtcaccctgt cggatcctgc gctggtggag
      901 gagatccaca gggcctacct gagtgccggg gcggagttga tagaaaccaa cagtttcggg
      961 gcaaaccggt tcaagctggc cgattacggg ctggagaatc gcgtcaggga gatcaacctc
     1021 aaggctgcca agctggccag gagcactcgg gagatcgcag gtgtggagtg tttcgtggcg
     1081 ggctccgtag ggccgttggg gaggccgctg gcacctgttg gacagatccg acaggctgcc
     1141 gcgtccgccg ccttccagga gcagatcggt gccctgctgg agggtggggt cgatctgata
     1201 ttactggaaa ccttccccga cctggaggaa ctggcgctgg ccctccaggt ggctcacgac
     1261 ctgtgcgatc tgcccgtcgt ggctcaggcg accttcgccg aggatgggct gacggcctct
     1321 ggctccactc ctgaagaggt tgtccggcga ctgcgcgata tgggtgcggc ggtggccggc
     1381 gtgaactgca gccttggccc tcagcagatg ctggagatcg ttcagcggat gattgccgag
     1441 ggcggggttc ccatctccgc catgcccaac gccgggttgc cggcaatggt cggtggccgg
     1501 ctcatctacc gaagcacgcc cccctatatg gccgagtatg ccctcaagta tgtgcatgag
     1561 ggtgcctcta tggtgggcgg ctgctgcggg accactcccg cccacattgc cgccatgcgg
     1621 accgtcctgg cggagttgag gccggcggag ccgcttcccc ctagctgcac cctgatcctc
     1681 gacgagtcgc caactccccc tgccgtgggc gagccgtcac cactgaaggc caagctgggg
     1741 aagcagttcg tcgtcagcgt cgagatggat ccccccaagg gcgccaaccc caccaagctg
     1801 ctgaatgcgg cttcggtact gagagaggtt ggcgtggatg cggtgaacat tgccgacagc
     1861 cctatggcgc gggtgcgcat gagtgctatg gccatggggt tcctggtgaa gcagcagttg
     1921 ggattggaga ccataatcca cttcaccact cgcgatcgaa acctgatggc gatccaatcg
     1981 gagttgatcg gcgcccacgc aatcgggatc agggcgatcc ttgcccttac cggtgatccc
     2041 ccgcgactag gcgaccagcc gggcacgacc gccgtcttcg acgtggactc tatcgggttg
     2101 atccgaatca tccggcgcct gaacgagggg caggactggc gtggcaactc catcggcgaa
     2161 cccaccaact tcctcgtggg ctgcgcgctg aaccccaacg ccgacgactt ggagttggag
     2221 ttggatcgct tccggcagaa gttggaggcc ggcgtggact tcgtgttgac gcagccggtc
     2281 tacgacatgg agaccttgct tcgtgtgctg gatcgagtgg agggggtgga ggcgcccatt
     2341 ctgctgggcg tgttgccgtt gcagagctct cgccacactg agtacctcca caacgaggtc
     2401 ccggggatta ccgtcccaga tccgatccgc gagcggatga ggcaggccgg tgagcatggg
     2461 atggacgagg gtgtgactat cgcccgggag ttcctccgag aggctcgcag ctacgtccag
     2521 ggtgcctacc ttatgccatc cttcggccgc ttcgagcagt gcgctgagat cttgcggtag
     2581 caacagctta accgtatacg aacgtaacca caaaggcacc acgccacaag ggatcatgag
     2641 tcttcattgt gacgtggtgc ctttgtccgc gatacccacg agggtttgtg ggtatcgcgg
     2701 acctgcgttg tcttgtgcgg ttatctcgtc agggatagaa cggtgtaagc gccggggcca
     2761 gccagggc
//
LOCUS       DATALS010000363         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_395834, whole genome shotgun sequence.
ACCESSION   DATALS010000363 DATALS010000000
VERSION     DATALS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395834"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VHS06_08140"
     CDS             complement(<1..228)
                     /locus_tag="VHS06_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988124.1"
                     /translation="MSRVLVRLGLSRESFARLSPKLRQRVVELLLRWQYEEDAIPLVE
                     MMVQADPNGLLHRSHLVGCLAACGRFEEAQSA"
     gene            406..1362
                     /locus_tag="VHS06_08145"
     CDS             406..1362
                     /locus_tag="VHS06_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2988125.1"
                     /translation="MNSKEEGLRLIKAGEYDEAARILDQVVETDPQDAQAFLYLGVAY
                     AEGGKKAESIAAFEQCVSVKPSTRGHYNLGLAYESVERLGEAMTQFENAARLDPSYQP
                     ALEAIERLKKQGIEARESTVQWTIGAQSVGQAMASAQAQTQVQDQTEQVLIGQKPPAA
                     PGGYTPGSLQSAMQGPKPGGPGPYLPGQLEAEKEAKIRAHQAQMLKSGLIYGAVIGAI
                     ARLAGQVFFGLIAPTVVIANCLAGAFFGAIIGAACGYTGGGDETGSKVGAVLFALLAV
                     VSGLIQHADIVFIIIPALVNAVIGFFVGGYIGKMVAQSIGWD"
     gene            1372..1620
                     /locus_tag="VHS06_08150"
     CDS             1372..1620
                     /locus_tag="VHS06_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2988126.1"
                     /translation="MDVTHSLLLLILVLAAAKVGGEIAERLGQPAVLGELLAGMLLGL
                     TQLKASAHDPAIVFVATIGIVLLLFEVGVGWYPEGRSV"
     gene            1593..1823
                     /locus_tag="VHS06_08155"
     CDS             1593..1823
                     /locus_tag="VHS06_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988127.1"
                     /translation="MVPRGEVGLIFASVGQKAGILPPELYAQVILIVLGTTLITPPWL
                     KSVFGSGARVRRSGRRRRDGRASDQSSVTSDQ"
     gene            complement(1856..2047)
                     /locus_tag="VHS06_08160"
     CDS             complement(1856..2047)
                     /locus_tag="VHS06_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2988128.1"
                     /translation="MKRRNFSKEFKLRVLSEVQSGRGVAEVARQYQLHPEQISSTIGY
                     VPPAEYEQRILMQRQAALI"
BASE COUNT          458 a          581 c          645 g          398 t
ORIGIN      
        1 cgcggactgt gcctcttcaa agcgcccgca ggccgcgagg cacccgacca gatgagagcg
       61 gtgaaggagc ccattgggat ccgcctgcac catcatctcc accagcggga tcgcatcctc
      121 ctcatactgc cacctcagga gcaattcgac cactctctgg cgcaatttgg gcgaaagcct
      181 ggcaaagctt tcccgtgaca gcccgagtct tacaagaacg cggctcactc gaccgctcct
      241 ttcctattgt tgagcggaaa tgataatgcc ggtagacaaa ataccgccca ccaacgcata
      301 atgtcaagtg ttggagagga atggatgagc atttgttgaa ggaagaacta cgcgaagtgt
      361 agcccgggac acatcgggca cacagaggga atgggaggga tagggatgaa tagcaaagag
      421 gagggtctgc ggctcatcaa agctggtgag tacgatgaag cagccagaat cctcgaccag
      481 gtagttgaga cggacccgca ggatgcacag gcgttcctct acctgggagt ggcctacgcc
      541 gaaggaggca agaaggccga atccatcgcc gcattcgagc agtgcgtctc agtcaagccg
      601 agcacccgtg gacactacaa cctggggctt gcctacgagt ccgtagaacg cctcggagag
      661 gctatgacgc agttcgagaa tgcagccagg ctcgacccca gctatcagcc ggcgctggaa
      721 gcgatcgagc ggctgaagaa gcagggcatc gaagcgcgcg agtccacggt ccagtggacg
      781 ataggcgcgc agtccgtagg ccaggcaatg gcctcggcgc aagcccagac acaggttcag
      841 gatcagacag agcaggtgct tatcggccag aagccgccgg cggcccctgg agggtacacg
      901 ccgggcagcc tgcaatctgc gatgcagggg ccaaaaccgg gagggccggg cccttacctg
      961 ccagggcagc ttgaggctga gaaggaagcg aagatccgcg cccaccaggc gcagatgctc
     1021 aagtccggcc tcatctatgg ggcagtgata ggagcgatcg cccgtcttgc aggacaggtc
     1081 ttcttcgggc tcatagctcc gacggtggtt attgcaaact gcttggcagg agctttcttc
     1141 ggagctataa tcggcgcggc ctgcggttat accggaggcg gtgacgagac cggatcgaag
     1201 gtcggggctg tgctctttgc gctgcttgcc gttgtatccg ggctgatcca gcatgcggac
     1261 atcgtcttca tcatcatacc ggccctcgtg aacgccgtaa tcggcttctt cgtgggaggg
     1321 tatatcggaa agatggtagc ccagagcatc ggctgggact agattcccca tgtggatgta
     1381 acacactcac tactacttct aatactggtt ctggcggcgg ccaaggtggg aggcgagatc
     1441 gctgaacgcc ttggccagcc tgccgttttg ggcgaactgc tggccgggat gctgcttggg
     1501 ctgacgcagc tcaaagcctc cgcgcacgac cctgcaatcg tcttcgtcgc caccatcggc
     1561 atcgttctgc tcctcttcga ggtgggcgtg ggatggtacc cagaggggag gtcggtctga
     1621 tattcgcaag cgtaggacag aaggccggca tcctgcctcc ggagctctat gcgcaggtga
     1681 tactgatagt cctgggaacg acgctgataa ctccaccgtg gctcaagagc gtgttcggaa
     1741 gcggcgcccg ggtgcgcagg agtgggagaa ggcggaggga cgggagagcg agtgatcagt
     1801 catcagtgac tagtgatcag taactgggac tgcactcctg gaatgagaca agtagtcaaa
     1861 tcagggctgc ttgtctttgc atcagtattc tctgctcata ctctgccgga ggcacatagc
     1921 cgatggtaga gctgatctgc tcaggatgca gctgatactg cctggctact tcggccacac
     1981 cccttccaga ctgaacctca gacaagacgc ggagtttgaa ctccttgctg aagtttctcc
     2041 tcttcatggt gacacctctt tctcatgata tcaccctgac ac
//
LOCUS       DATALS010000364         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_223189, whole genome shotgun sequence.
ACCESSION   DATALS010000364 DATALS010000000
VERSION     DATALS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223189"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1876)
                     /locus_tag="VHS06_08165"
     CDS             complement(143..1876)
                     /locus_tag="VHS06_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX2988129.1"
                     /translation="MVLESRRPVGLSHGASPSVADQAVGAAAFQRSESGDDIAVVAGF
                     LRGLVEGDGDLKTRLRLSPNSPMREVGQLLNRFVEDTAKLLSQLDEHTNRVGVGVAQN
                     VRNIAGTARNQAQEIDRVAQALVQARQAGNDVAEAANRASGSSESTREMAARGNDAVE
                     ATGEGVERSRNSAQLSAGTVKELITSSSEIDKITEAIEDIAEQTNLLALNAAIEAARA
                     GEHGRGFAVVAAEVRKLSERTRDSTKEISRMVKGLQTQMSSLSGVIATNVQEAEMAVD
                     KAATSRSTLSEISRLAVRSTEEMANVAAGNQEIAATIDDVAARVGKLSDSAKLVADNM
                     DATATSEDTEMAIIEIHRLLARYRLGTFTEQVREWAQACALEVAAALESAIDRHKLTL
                     DQLMDWRYEEIKGPAIQRLSRLFDVRRVPPEGFNPPKFATSWDHLMDVQMRTVLDGYV
                     AKHRRLNNVYISDVNGYLFTHLTRYTQPWTGNRKEDINHNRAKRIFDQQVALRATRVG
                     IKGWDRVPKRATRQQFLSTGVNPDQPVSPEIFVLQTLARASGDTVCDLAVPLFVKGRR
                     YGAVRFSFLAK"
     gene            complement(1927..2469)
                     /locus_tag="VHS06_08170"
     CDS             complement(1927..2469)
                     /locus_tag="VHS06_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988130.1"
                     /translation="MTILEIKGPDIQRLSHLIDVRRAPQEGFKPPKYATSWDHLLDVQ
                     VRAVLDRYITKDKRINNVCVPDVNGYNFTHLSKYCGAWTGNPKIDGVSNRAKWLTDYP
                     VVLRAARVGLNDWDKVPKRANRAQFLAARVDPDQKLPPDCFLLQTQARDTGDTVCDLA
                     VPLFVKGRRYGAIRIGFLAR"
BASE COUNT          449 a          833 c          744 g          492 t
ORIGIN      
        1 gggccgacga actcaagcag gccgaaaagc ggtacctacc ccaaagcagc accatcaacc
       61 gatagtgagt ttgggctcag acgagtcgtg gagggaatcg gtgaggggtg acgttcgcgc
      121 caccccttgc ctgaattccg acctacttcg ccaggaagct gaaccggacg gcaccatacc
      181 gccgtccttt cacgaagagg ggaacagcca gatcgcagac cgtgtcgccg gaagccctgg
      241 ccagggtctg cagcacgaag atctcgggcg aaacgggttg gtcggggttc acccccgtgg
      301 agaggaactg ctgtctcgtg gccctcttgg ggaccctatc ccaccctttg atgcccacgc
      361 gggtagcccg tagcgccacc tgctggtcga agatccgctt agcccgattg tgattgatgt
      421 cttccttgcg gttgccggtc cagggctgcg tgtacctcgt caggtgggtg aagaggtagc
      481 cgttcacgtc cgatatgtag acgttgttca gccgcctgtg cttagcgaca tagccgtcca
      541 gcaccgttcg catctgcacg tccatcaggt ggtcccagga ggtggcgaac ttgggcggat
      601 tgaacccctc aggcggaacc ctcctgacgt cgaagagccg agaaagccgc tggatcgcgg
      661 gccccttgat ctcctcgtag cgccagtcca tcaactggtc cagcgtcagc ttgtgccggt
      721 cgatggccga ctccagcgcg gccgctacct ccaaggcgca cgcctgagcc cactccctca
      781 cctgctcggt gaacgtcccc agccggtacc gggcgagaag ccggtggatc tcgatgatgg
      841 ccatctcggt gtcctctgag gtagcggtgg cgtccatgtt atccgccacc aacttggccg
      901 agtcggacag cttgcccacc ctcgcggcca cgtcgtcgat ggttgccgcg atctcctggt
      961 tgccggccgc gacgttggcc atctcctcgg tcgagcgaac ggccagcctg gagatctcgg
     1021 acagggtaga gcgggaggtt gcggccttgt ccaccgccat ctccgcctcc tgaacgttgg
     1081 tcgctatcac accggagagg ctcgacatct gggtctgaag ccccttgacc atcctggaga
     1141 tctccttggt gctgtcacga gtgcgctccg agagcttccg cacctcggcc gccaccaccg
     1201 cgaagccccg tccatgctct cccgccctgg ccgcctcgat ggccgcgttg agcgccagca
     1261 gattggtctg ctcggcaatg tcctcgatcg cctccgtgat cttgtcgatc tccgacgagg
     1321 aggtgatcaa ctccttcacg gtacccgcgg agagctgagc gctatttctg cttcgctcca
     1381 ctccctcacc cgtggcttcc accgcatcgt tacccctggc agccatctcc ctggtggact
     1441 cggagctgcc gctggcacgg ttggcggcct cagcaacatc gttgccggcc tgccgagcct
     1501 gcaccagagc ctgggcaacc cgatcgatct cctgggcctg gtttcttgcg gtcccggcga
     1561 tgttgcggac gttctgcgcg acgccgacac ccacccgatt ggtgtgctcg tctagctggc
     1621 taagcagctt cgcggtatcc tccacaaacc gattgagtag ctgcccaact tcccgcattg
     1681 gggaattggg cgacaaccgg agacgtgtct tgagatcgcc gtcaccctcc acgagacccc
     1741 tgagaaaccc cgctacaacg gcgatgtcgt caccggattc agagcgttgg aaagccgccg
     1801 ccccgacggc ctggtcggct acggaaggcg atgcgccatg actcagacct actggcctac
     1861 gagactcgag taccatgacg accctccacc ctgatactca cccacaccac tcaaccgcca
     1921 gcacccctac cgtgccaaga agccgatgcg aatggcgcca tatcgtctcc ccttcacgaa
     1981 taggggcacg gccagatcgc acacggtgtc gcccgtgtcg cgcgcctggg tctggaggag
     2041 gaagcaatct ggcggaagct tctgatcggg atcgaccctc gccgcgagga actgagcccg
     2101 gtttgcccgt ttgggaacct tgtcccaatc gttcagcccc accctggccg cacgaaggac
     2161 aaccgggtag tcggtgagcc acttggcccg gttgctcaca ccgtcgatct ttggattgcc
     2221 ggtccaggca ccacagtact tgctgaggtg ggtgaaattg tagccgttga cgtcgggcac
     2281 gcagacgttg ttgatccgct tgtccttggt gatataacga tccaacaccg ctcgcacctg
     2341 gacgtccagc aggtgatccc aggaagtggc atactttgga ggcttgaaac cctcttgagg
     2401 ggctcgccga acgtcgatga ggtggcttaa ccgctgaatg tcgggcccct tgatctccag
     2461 gatggtcaag gggcttcaga cccagatgtc gagcctctcc ggtgtgatag cgaccaac
//
LOCUS       DATALS010000365         8208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_39916,
            whole genome shotgun sequence.
ACCESSION   DATALS010000365 DATALS010000000
VERSION     DATALS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39916"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..672
                     /locus_tag="VHS06_08175"
     CDS             16..672
                     /locus_tag="VHS06_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988131.1"
                     /translation="MSGVSLEVEEGSVVGIIGSNGAGKSTIMRAISGLVKPSSGDIYF
                     DGKRINGLKPHEIVKLGVVQVPEDHRHFPHMSVIANLRLGAYLRKDKKAIEADLENVF
                     GHFPKLRARRNQKAGTLSGGELEILVIARGLMAKPRLLLLDEPSLGLSPIIIDEQSEV
                     IADINRQGVTVLLVEQNAGLVTEVTNRGYVLEVGKVVIEGNIDELMNNQSVQRAFIGG
                     "
     gene            718..2217
                     /locus_tag="VHS06_08180"
     CDS             718..2217
                     /locus_tag="VHS06_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2988132.1"
                     /translation="MPAEGAVMSEGGVAVRAAFAGAVPDEGITDDPRRLAGYCEDRSF
                     GTGATAQLVVYPTRKEELQAIVRQANEHRLPLVPVSSGPPHFHGGSRPAHGGVVADFQ
                     RMNRILKVDATDRYVMIEPGVTYGELIPEVRKKGLRLDAPLLPRASKSVVASRLEREP
                     VAIPKYQYDYSDPLMTVEVVYGDGTDFRTGSASGPGPLEQLTADKVNPWGPGAIDYLR
                     FITGAQGTMGLVTWAVTKAEVLPSVQKLYFITTSNLGLLITFMNRLLRRRVGDECLAL
                     NNVGLAARLAQDWSGDFERLRGSLPEWTALVVLAGYKHHPEERLAIQERYLFEVCHEL
                     GVAPLTTLPGAEGREDAVLATLSEAYCREPYFKLGPRGSCRDIMYLSPPSKVADLAGI
                     VEGLRAKHEFPRESVGAYLQPCVQGRGYHCEFELFCAEDDPIEMARVRGLFFDASEAL
                     MAGGAFFSRPYGPWSDMVYSRYGEGVAALRKLKGIFDPNNILNPGKLCF"
     gene            2228..3604
                     /locus_tag="VHS06_08185"
     CDS             2228..3604
                     /locus_tag="VHS06_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2988133.1"
                     /translation="MDELKDFLFTMLSCNHCGQCKWILGPKMRGWDFAEVCPIHQRYH
                     WDAYSGQGLVNIAQEVLEGTLAWDSGLLELIYSCTTCGACDINCKSVRDMEPLDTILA
                     LRAKCVADGQGPMPEHRAQADNLTREGNIYGRPRSERGAWLEWEGAGQPGLTTTNDAE
                     VAFFVGCGTAFNYPQTARDIARILQAGGVEFQVLGADELCCGAPLWRTGQVDAFAKSV
                     ARNLQAFRARGIKTLVTGCAECYGTFRGGYSRLDPMDDIEVLHITQVIARLLEEGRLN
                     LKASPKTDPDLKVTYHDPCLLARLSEKYVPWHGEIEAYGRHVPPKAWRRGTDGVYEAP
                     RTVLQAIPGVELVEMVRSEECGYCCGAGGGVSLSNPELAEWIAGERLREARASGAEAI
                     VSCCPFCRDNLTRDGGLPYYELTELVARALAGGRPARPPAAGLQAAMGTVQPTGPAQA
                     PEAARGDR"
     gene            3601..5121
                     /locus_tag="VHS06_08190"
     CDS             3601..5121
                     /locus_tag="VHS06_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2988134.1"
                     /translation="MTLPRHIYQMLEGVVGAEYISEAPCIMAGYRRSGPHATLSPSPE
                     VVLLPASTEEIQAIVKICNRYEIAYIATVSGLSVQAYPDRPATIVLHLKRMNRIVEIN
                     DTDRYAVIEPGVRHGQLKPELMKRGFTYTVASVGPGGSVLANFTTTSGDHHNQNGLSR
                     ANRYLLGVEWVTPSGDIVRTGSLGAGAGWFCPEGPGPSLRGLMKGYTGSFGALGIITR
                     VAIGLEPSKGPEVMSAQGRTPKYTFNLPRDRHRAFIFKFPDLDKVRDAMLEVGRAEIG
                     HSVLKFFNATYALLNTESANDFWDLWDSGLFQRELSKPLYVYLATWSEEEMEYEERVL
                     REIVAELGGEPVDERIQKIQEDNMDFYIIVSFLQRTLRLGGGWAPNKLSADSVTHMFE
                     VGKAIPGMLAEFIDSGRLLNAPDNFQISPMEYGHMAHIELLFVWDFGWPDWPSIPMEV
                     MKKSAADDIAAGRHAAMPGRDPMAMRRDGPLYSGFDRWALAIKEAFDPKGLANPSP"
     gene            5792..6610
                     /locus_tag="VHS06_08195"
     CDS             5792..6610
                     /locus_tag="VHS06_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2988135.1"
                     /translation="MTNKVVSKDGTTIGYTSVGRGPAVLVIPGVLSMAADYAAFASAL
                     GDSFTVHVIERRGRGESGPQGDDYSVVKECEDLLALQHETGASLVVGHSYGGLVALEV
                     ARNTAAFTKVVVYEPGVSISGSMGANRMSSYERKLAAGRNLDALAEYALAVGPRRMQG
                     WPTWLMKLMLSLNLVWHPEQRHMLDLLPQLRREWGEIARLDSSYENYREVVADVLLMY
                     GGRSDSLAVDLQVERLPAILSSFKTRQFPTLDHFGIERTAPREVADAVAGYFVE"
     gene            6845..7144
                     /locus_tag="VHS06_08200"
     CDS             6845..7144
                     /locus_tag="VHS06_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988136.1"
                     /translation="MIGMDEQERREGQLEAWELQQLKESGSEKMGDVSPDVDEELVLV
                     GDRGPVPYTDLLTEEEYRDFRELAEREEPTPDEVARGQALLRLMMNRYPDGPVSR"
     gene            complement(7348..7656)
                     /locus_tag="VHS06_08205"
     CDS             complement(7348..7656)
                     /locus_tag="VHS06_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988137.1"
                     /translation="MKDRFRDQPREVKSACRVARDADDEAWVYCASCHRAMRQGDCVL
                     EGLESGLSCAYDDCRPEGNLAFKSLYGWDAYRLAHQRETGQWPIEPESGECYEPGATP
                     "
     gene            complement(7659..7859)
                     /locus_tag="VHS06_08210"
     CDS             complement(7659..7859)
                     /locus_tag="VHS06_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988138.1"
                     /translation="MATCAQMKMGEIYACPDCGVELQVVKECADEGKCAVDHCRLTCC
                     DKEMELRSHAPQESSWADEAGG"
BASE COUNT         1552 a         2471 c         2662 g         1523 t
ORIGIN      
        1 gacaccgccg aggcgctgag cggcgtctcg cttgaggtgg aagagggctc ggtagtgggc
       61 atcatcggct ccaacggagc gggcaagagc acgatcatgc gggcgatctc ggggctggtg
      121 aagccgagta gcggtgacat ctacttcgac ggcaagagga tcaacggcct caaacctcac
      181 gagatcgtga agctcggggt ggtacaggtg cccgaggacc accggcactt cccgcatatg
      241 tcggtaatcg ccaacctccg tctgggcgcc tacttacgca aggacaagaa ggccatagag
      301 gccgacctgg aaaacgtgtt cggccacttt cccaagctcc gtgcgcgccg caatcagaag
      361 gccggcacgc tgagcggcgg cgaattggag attcttgtca ttgcccgagg actgatggca
      421 aagccgaggc tgctgttgct cgatgagccg tcgctcggct tatcgccgat cataatcgac
      481 gagcagagcg aggtaatcgc ggatatcaac cgccagggag tgactgtgct tctggtggag
      541 cagaacgctg gattggtgac ggaggtgacc aaccgaggct acgtgctcga ggtcggtaag
      601 gtcgtgatcg agggcaacat cgacgagctg atgaacaacc agtcggtaca gcgcgcgttc
      661 atcggcggct agtcgctcag gccgagccac tggcgcggcg aggagtacag gtctgtcatg
      721 ccggcagagg gagctgtcat gtccgaagga ggagttgcgg tccgagcggc tttcgccggg
      781 gccgttccgg atgaggggat caccgatgac ccgcgccgtc ttgcaggata ctgcgaggac
      841 cgtagcttcg gcaccggggc aactgcgcaa cttgtggtgt atcccaccag gaaggaagag
      901 ctgcaggcga tcgtccgaca ggccaatgag caccggctcc ccttggtgcc cgtaagctcg
      961 ggtccgcctc actttcatgg aggcagccgt ccggcgcacg gtggggtagt agcagacttc
     1021 cagcgaatga accgcatcct gaaggtggac gccaccgacc gctacgtgat gattgagccg
     1081 ggcgtcacct acggtgagct gatccctgag gtgcggaaga agggactgcg tctcgacgcc
     1141 ccgcttctgc cccgcgcttc gaaatcggtt gtcgccagcc gccttgagcg cgagcctgtg
     1201 gcgattccca agtaccaata tgattacagc gacccgctga tgaccgtaga ggtcgtgtac
     1261 ggcgacggaa cggacttccg taccgggtcg gcgtcaggcc ccgggccgct ggagcaactc
     1321 acggccgaca aagtgaaccc ctggggcccg ggcgcgatag attatcttcg cttcatcacc
     1381 ggggcccagg gcaccatggg cctcgtcacc tgggcggtga ccaaggcgga agtcctcccc
     1441 tccgtgcaga agctgtactt catcaccact tcaaacctcg gtttgctgat cacgttcatg
     1501 aatcggctcc tccggcgccg ggtaggggac gagtgcctgg ccctgaacaa cgtcgggctg
     1561 gcggcgcgcc tggcccagga ctggtccggc gactttgagc ggctccgcgg atcacttccg
     1621 gagtggacgg cactcgtagt gctggccggc tacaagcacc acccggagga gcgtctggcg
     1681 atacaggagc gatacctgtt cgaggtctgt cacgaactgg gagttgcgcc gctcaccact
     1741 ctgccggggg ccgagggccg cgaggatgcg gtcctcgcca cgctgtccga agcctactgc
     1801 cgcgagccct acttcaagct agggccccgg ggttcgtgcc gggacatcat gtacctctcg
     1861 cctccgagca aggtcgccga cctagccggg attgtcgagg ggttgcgggc gaaacacgag
     1921 tttcctcggg agagcgtggg cgcgtacctg cagccctgcg tccagggccg gggttatcac
     1981 tgtgagttcg agctcttctg tgctgaggac gacccgatcg aaatggcccg cgtccgcggc
     2041 ctgttcttcg acgcttcgga agcgctcatg gctggcggtg cgttcttcag ccgtccttac
     2101 ggcccctggt ccgacatggt ttacagccgc tatggggagg gagtcgctgc cctcaggaaa
     2161 ctgaagggca tctttgaccc caacaacata ctcaacccgg gcaagctctg cttctaggag
     2221 gtcacgggtg gacgaactga aagacttcct tttcacgatg ctctcctgca accactgcgg
     2281 ccagtgcaag tggattttgg ggccgaagat gcggggctgg gatttcgccg aggtgtgtcc
     2341 gatccatcag cgctaccact gggatgcgta ctcggggcaa ggactggtga acatcgccca
     2401 ggaggtgctg gagggaacgc tcgcctggga ttccggcctg ctcgaactca tctacagttg
     2461 cacgacgtgc ggcgcgtgcg atatcaactg caagtccgtc cgcgacatgg agccgctgga
     2521 cacgatcctg gcgctccgcg cgaagtgtgt ggccgatggc caaggaccga tgccggagca
     2581 ccgagcgcag gccgacaacc tcacgcggga gggcaacatc tatggacggc cgcgtagcga
     2641 acgaggggcc tggctggagt gggagggagc tggccaaccg gggttgacga cgaccaacga
     2701 cgcagaggtc gccttcttcg tcggctgcgg taccgctttc aattacccgc agaccgcccg
     2761 ggacatcgcg cgcatcctac aggcgggtgg ggtggagttc caggtgctcg gtgccgacga
     2821 actctgctgc ggagcgccgc tctggcgcac cggccaggtt gacgcatttg cgaagagtgt
     2881 tgctcgcaac ctgcaggcgt ttcgagcacg ggggatcaag accctcgtga ccggctgtgc
     2941 cgaatgctac ggcacgttcc gaggcggata ctcccggctc gacccgatgg acgacatcga
     3001 ggtgctccac atcacccagg tcatcgcgcg cctgcttgag gagggtcggc tcaatctcaa
     3061 ggcctccccg aagacggacc ccgaccttaa ggtcacctat cacgatccgt gtctgctggc
     3121 ccgtctttcc gagaagtacg tcccctggca cggggaaatc gaggcgtacg gccggcacgt
     3181 gccgccaaaa gcctggcggc gaggcaccga tggcgtctac gaggcgccgc gaacggtgct
     3241 gcaggctatc cctggcgtcg aattggttga aatggtgcgg agcgaggagt gcggctactg
     3301 ctgcggtgcc ggaggaggcg tctcgctgtc gaaccccgaa ctcgccgagt ggatcgccgg
     3361 ggaacggttg cgagaggcgc gggccagcgg ggcggaagcg atcgtctcgt gctgtccttt
     3421 ttgccgcgac aacctgaccc gcgacggcgg actgccgtac tacgagttga ccgagttggt
     3481 ggcccgggct cttgcgggtg ggcgacctgc gcgcccaccg gcggctggcc ttcaagcggc
     3541 gatgggcacg gttcagccga cgggccccgc acaggcgcct gaggccgcaa ggggggatcg
     3601 gtgactcttc cccgccacat ctaccagatg ctcgagggcg tggtcggggc tgagtacatc
     3661 agcgaagcgc cctgcattat ggccgggtat cgcaggtcgg gaccacacgc gaccctctcg
     3721 cccagtcccg aagtcgtgct cttaccggcg tctactgaag agatccaagc gatcgtcaaa
     3781 atctgcaatc gatacgaaat cgcctacatc gcgacggtct ccgggctttc cgtgcaggcc
     3841 taccccgatc ggccagccac catagtgctt cacctcaagc gaatgaaccg cattgtggag
     3901 atcaatgata ccgaccgcta cgcggtgatt gagccgggag tgcggcatgg tcagcttaag
     3961 cctgagctga tgaagcgagg tttcacatac acggtcgctt cggtgggccc tggcgggtcg
     4021 gttctagcca acttcaccac gacgtccgga gaccaccaca accagaacgg gctctctcgg
     4081 gcgaatcgct accttctggg cgtggaatgg gtcaccccca gcggagacat tgtacggacg
     4141 ggttcgctcg gcgcgggcgc gggatggttc tgcccggaag gcccggggcc cagccttcgt
     4201 ggcctcatga agggctacac cggcagtttc ggagcgctgg gaatcatcac cagagtggca
     4261 atcggcttgg agccctcgaa aggcccggag gtgatgtcgg cccaaggccg caccccgaag
     4321 tacaccttta acctgcctcg cgaccggcac cgcgcgttca tcttcaagtt tcccgacctc
     4381 gacaaggtcc gggatgccat gctggaggtc ggccgggcgg agatcggaca cagtgtgctg
     4441 aagttcttca atgcgaccta cgctctcctt aatacggagt cggccaacga cttctgggac
     4501 ctgtgggaca gcgggctctt ccagcgggag ttgtcaaagc cgctgtatgt ctacctggcc
     4561 acctggtccg aagaggagat ggagtatgaa gagcgggtac ttcgcgaaat cgtcgccgaa
     4621 ttgggcggcg agccggtcga cgagcggatc cagaagattc aggaagacaa catggacttc
     4681 tacatcatcg tgagcttcct ccagcggact ctccgattgg gaggcggctg ggccccgaac
     4741 aagctgagcg cggattctgt cactcacatg ttcgaggtgg ggaaggccat cccgggcatg
     4801 ctcgcggagt tcatcgacag cggtcgcctg ctcaacgctc ccgacaactt ccagatcagc
     4861 ccgatggaat acggccacat ggcccatatc gaactgctct tcgtctggga cttcggttgg
     4921 cccgattggc cgagcatccc gatggaggtc atgaagaaat cggccgcgga cgatatagcg
     4981 gccggccggc atgcggccat gccggggcgc gatccgatgg cgatgcgccg cgacggcccc
     5041 ctctacagcg gcttcgaccg ctgggctctg gcaatcaagg aggcgttcga cccgaaagga
     5101 ctggcgaacc caagtccttg aggcgagaag cgcctccagc caacggttgg cgggccgggc
     5161 agtttcttgc cgagaggtcg atgaggcgcg gtcgtgcgca cacatcggac ctagacgttt
     5221 tcccaacatg accgcaccgt ctcagagtca cgtgcgttcc cgcctccgcc gggcggcccg
     5281 taccatttag ggaaagtgcc cagctgccca tctccccgaa gccgcgcatc ccgatcgagt
     5341 ctgggatctg gggtccctat cttcctcttg gcgctcgacg caagccatcc gtcgacgcac
     5401 gctctgccta caatcgaccc cgttcgctta ccgattgcct ggctgccatg tgtgtacgct
     5461 cagtcagttc agtcagtcca gatccggctc gccttcccga tgagaagcgg ctgtacttga
     5521 gcgacattgg tggtagacgt ccttgggcgg cacactaacc gttcaatatc ttcgtcgcca
     5581 gatcgggctt cgggccaagc gacgcacaag cccacgagaa ccagactggt caactggctg
     5641 atgcagagcc caccagatgg ccgtttcaac ttcgacccgg atccgtgcat attgagaata
     5701 aggcacgctt cgggtgagcc atcccgcgcc cgggtaattg ctttcccgag cccgggttgg
     5761 ccgcaaggga agggatgacc ggaagatgag catgacgaac aaggtagtct caaaggatgg
     5821 aacgacgatt ggctatacct ctgttggacg agggccggcc gtccttgtaa ttccaggagt
     5881 actctccatg gctgccgatt atgctgcctt tgcgtcggcc ctgggtgatt ctttcacggt
     5941 ccacgtcatc gagcgtcgcg ggcgaggtga gagtggcccc cagggcgatg actacagcgt
     6001 ggtcaaagag tgtgaggacc tgcttgcgct gcagcatgag accggcgcgt ccctggttgt
     6061 tggccacagc tacgggggac ttgtcgccct ggaggttgcc cgtaatactg cggcgttcac
     6121 caaggtggtc gtgtacgagc caggagtgtc gatcagtggg tccatggggg cgaaccgcat
     6181 gtcctcgtat gagaggaaac tcgccgcggg cagaaacctc gatgcgttgg cggaatacgc
     6241 cctagccgtc ggccctcgac gcatgcaggg atggcccacg tggctcatga aactgatgtt
     6301 gtccctcaac cttgtgtggc atccggagca gcgacatatg ctcgatctcc tccctcagct
     6361 taggcgagaa tggggagaga ttgctcggct tgatagcagc tacgaaaact accgagaagt
     6421 ggttgctgac gttcttctca tgtacggcgg gcgtagcgac agcctagcgg tcgatctcca
     6481 agtcgagcga cttcccgcga tactttccag ctttaagacg agacaattcc ccacactcga
     6541 ccactttggg atcgagcgaa cagccccgag agaagtagcc gacgcggttg ccggctactt
     6601 tgtcgaatag gccgtacgag cggcggatcc gacccgcagc aagcctctgt tggcggcacg
     6661 actcgggttc ggttgctttc tcctgggtcg agtgtgatca cggactggta ttggctcggt
     6721 ccggttaggc gcactggtga cttgccaatg actctcgagt gaggcgacgg gtaatctagg
     6781 ggctagagca caattcaaag atagcgagag cgtactgtct aaccgggatg gcccgggcga
     6841 gaggatgata ggcatggatg agcaggagcg gcgcgagggg caattggaag catgggagct
     6901 tcagcaactg aaggaatcgg gctcggagaa gatgggggac gtgtctccgg acgtggatga
     6961 ggaactcgtt ctcgtgggcg atcgtggccc cgtcccctac acggatctcc tgacagagga
     7021 ggagtaccgg gacttccgag agcttgccga gagggaggag cccacgcctg acgaagtggc
     7081 ccgtggccag gctcttctcc ggctcatgat gaaccgctac ccggacggtc ctgtctcaag
     7141 gtaggtctgt ctgaacgcgg ggctctgctg ggagagtccg ttgaagtggc aagcacgcgc
     7201 tggcgccggc ctgactatga atcagagggt agtcagcgac caatgcgctt atcctcggtt
     7261 atgaaaagct ctgactgacc acgacatcgc cagtcgagtc aagcaacact cgggatgcgt
     7321 acggataagg tgcgcccgcc ttgtggatta cggagttgcg cctggctcgt agcactctcc
     7381 ggactcgggc tcgatgggcc actgaccggt ctctcgctgg tgagccagcc gataggcgtc
     7441 ccacccatag aggctcttga aggccagatt gccctcgggc cggcagtcgt cgtaggcaca
     7501 cgagagtccg gactcaagcc cttcaagcac gcagtcccct tgccgcatgg cccgatggca
     7561 ggacgcgcag tacacccagg cttcgtcgtc tgcgtcgcgg gcgactcgac acgcgctctt
     7621 gacctctcgc ggctggtcgc ggaaccgatc cttcaccgtc agcccccggc ctcgtccgcc
     7681 cagcttgatt cctggggggc gtggcttctc aactccattt ccttgtcgca gcacgtcagc
     7741 ctgcaatggt ctaccgcgca tttgccttcg tcggcgcatt ctttgacgac ctgaagttcg
     7801 acgccgcagt cggggcaagc gtagatctcg cccatcttca tctgagcaca agtcgccatc
     7861 ggtcctcctt ctaggcagtc tgttcgctgt gtgctacccg cagctctcgg cggggaaacg
     7921 cgggtgcgtt actttcgcgg gtcggctcgc ggacgactcg tcagtgggtt ggcgcgaaga
     7981 agccgaccgt ctcttagtga atcgagcaga ccgacgatgt atcagtaggc tgcagtctga
     8041 tccccatcgc ccgctgtcga gaaaagcctg tggaaagggc atgtgactgg ccccgcccgg
     8101 cccccaaggc acacgtggag ggtttagact gctcctccca ctgcgcatgt atgaagcgcc
     8161 acaatcatca gaggtcgcca gaagctacaa gagggcacca gggcggca
//
LOCUS       DATALS010000366         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_334035, whole genome shotgun sequence.
ACCESSION   DATALS010000366 DATALS010000000
VERSION     DATALS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334035"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..251)
                     /locus_tag="VHS06_08215"
     CDS             complement(27..251)
                     /locus_tag="VHS06_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX2988139.1"
                     /translation="MEEKVAKALESIRPGLQMDGGDIELVGVEDGVVKVRLVGACAGC
                     PMSMMTLQMGVERAIKKEVPEVVRVEAVPF"
     gene            complement(334..942)
                     /locus_tag="VHS06_08220"
     CDS             complement(334..942)
                     /locus_tag="VHS06_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2988140.1"
                     /translation="MQKTRRQILDLLKLRGSATLEELSREIGLSSVTIRAHLSVLERD
                     DLITSEEVRGRVGRPHFLYSLTESAQDQFPVSYDIVAHRFLEAFRSIATEEQRELLVR
                     VVAERWAAEKGGRLQDKGLEERVAEVARIRSEEGAMAEWQKTGDGYLLRQHHCTAGRI
                     SGGHPEVCQAELEYLRRLLGVYVERVESPHDGRTKCSYLVKP"
     gene            complement(1064..1429)
                     /gene="folB"
                     /locus_tag="VHS06_08225"
     CDS             complement(1064..1429)
                     /gene="folB"
                     /locus_tag="VHS06_08225"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX2988141.1"
                     /translation="MAEDRIILQGMVFFGYHGVNPEERTLGQRFELDLELEMDLSAAG
                     LSDDLSRTVNYASAYKLAREVVEGQPYNLIEAVAERLASAMLSRFPVEGVRVRLRKPW
                     APIKGSVLDSVAVEIVRKR"
     gene            complement(1516..>2085)
                     /locus_tag="VHS06_08230"
     CDS             complement(1516..>2085)
                     /locus_tag="VHS06_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2988142.1"
                     /translation="RDENELQHLVHTDLRHCSDGRGAFAVRYPRGAGTGDEMDEDLRC
                     LPLGKGELLRERDDLAIVAIGSTVRQALGAADLLAEQGVHCTVVDARFLKPLDEDLIL
                     SVAQRCGQVMTVEENVLAGGFGSGVLELISSRRLGGIQVEVVAIPDEFVEHGQPQILR
                     QKYGLDAKGLVQRALSAFPRLGMAAKVGA"
BASE COUNT          407 a          771 c          522 g          385 t
ORIGIN      
        1 gccaccccct ctttgtgtct taatccctag aagggcacgg cctcgacccg caccacctca
       61 gggacctctt tcttgatcgc gcgctcgacg cccatctgca gtgtcatcat cgacatgggg
      121 catccggcac acgcaccgac cagacggacc ttcacgacac cgtcctcgac gccgacaagc
      181 tctatatcgc caccgtccat ctgcaggccc ggcctgatgg actccagtgc cttggcaacc
      241 ttctcctcca acggtataca cccctttcga caacaatcgc taagcgcagg ctatgctacc
      301 agcagccaac aaagccgtca attgaaacca accttacggt ttcaccaggt agctacattt
      361 cgtccgccca tcgtgagggc tctccactcg ctccacgtag acacccaata gtcggcgcag
      421 atactccagc tcagcctggc acacctcagg gtgtcccccc gaaatccggc cggccgtgca
      481 atggtgctgg cgcagcaggt agccgtcgcc ggtcttctgc cattccgcca tggcaccttc
      541 ctcgctgcgt atccgcgcaa cctcggcgac ccgctcctcc agccccttat cctggagtcg
      601 gccgcccttt tccgcggccc accgctccgc cactactcta acgagcaact ctcgttgctc
      661 ctccgtggcg atgctccgga aagcctccag gaagcgatga gcgactatgt cgtacgaaac
      721 cggaaactga tcttgtgctg actcagtaag cgaatagagg aagtgagggc gtccgacccg
      781 accgcgaacc tcttcagagg tgatcagatc gtccctctcc agaaccgaga gatgggctcg
      841 aatggtaacc gaggaaagac cgatctccct ggataattcc tccagggtgg cgcttcccct
      901 caacttgaga agatccagga tctgtcgccg cgtcttttgc accgcagcct cctcatgggg
      961 tatctcggga agccgcccaa gcgtggccag ccccgacaag agtggtgtag cccatcaagc
     1021 ctaacagaag atggagagct ttgtcaatta gcaaacatag atatcagcgt ttccggacga
     1081 tctccaccgc cacgctgtcc agcacgctcc cctttatcgg cgcccacggc ttccgcagcc
     1141 gaacccgcac cccctccacc gggaatcggg agagcatcgc cgacgccaac cgctccgcga
     1201 cggcctcgat caggttgtaa ggctgtccct ccaccacctc tctggccagc ttgtaggctg
     1261 aggcgtagtt caccgtccgc gacaggtcgt cggacagccc ggccgcggag agatccatct
     1321 ccagctctag gtccaactcg aagcgctgtc ccagcgttcg ctcctcgggg ttcaccccgt
     1381 ggtagccgaa gaataccatc ccctgcagga ttatcctgtc ctccgccatc ttcccaccca
     1441 cagtgtaggg gcgacgcgtg cgtcgcccct accgatatcg tctgctatcg cctatgccgg
     1501 ccacacccca acccctcagg cgccgacctt cgccgccatt cccagccgcg ggaacgcgga
     1561 aagcgccctt tgaaccagtc ccttcgcatc cagtccatac ttctgtctga ggatctgcgg
     1621 ctggccgtgc tccacgaact cgtcgggtat cgccaccacc tccacctgga taccgccgag
     1681 cctgcgggac gagatcagtt ccagcacgcc ggagccaaag ccccctgcca gaacgttctc
     1741 ctccaccgtc atcacctgac cgcatcgctg agcaacactc aggatcaaat cctcgtccag
     1801 cggcttcaag aagcgtgcgt ccaccacggt gcagtgcaca ccctgctcgg caagcaggtc
     1861 cgctgcgccc aacgcctgcc tcacggtgga gccgatggcc actattgcca gatcgtccct
     1921 ctcccgaagc agctcaccct tcccgagggg caggcaccga agatcctcgt ccatctcatc
     1981 gccggtgccg gcgccccgcg gataccgaac ggcgaaggct ccgcgcccgt cggaacagtg
     2041 ccgcagatcg gtgtgaacca gatgctgcaa ctcgttctcg tctcg
//
LOCUS       DATALS010000367         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_109321, whole genome shotgun sequence.
ACCESSION   DATALS010000367 DATALS010000000
VERSION     DATALS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109321"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VHS06_08235"
     CDS             complement(<1..724)
                     /locus_tag="VHS06_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2988143.1"
                     /translation="MKITILGLGPGPYPLLTQEAREVLDQTPKLWLRTSRHPTVEKLP
                     KTVKWESLDYVYDRCESFDCLYRELAAEVISIARREGDIVYAVPGDPTVAEDSVREIL
                     KAAGDEGIEIQIVSGVSFIEVMLAAARTTLSGGLQLLDAAHVASANPLIDLMVYQIED
                     RLVASDVKLELLRKYPDDHPVVLVTAAGVPGEQAVHQVHLHDLDRQPSVDHLTALYVP
                     ASAPEQALGSFDSLVNIVAPMRP"
     gene            962..1507
                     /locus_tag="VHS06_08240"
     CDS             962..1507
                     /locus_tag="VHS06_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988144.1"
                     /translation="MPGNRGAARVVAFLLALSALALILSGCELVVVESSGVRGDAQRN
                     AMSTAVADLPDYDIAVSAIDFDPPLKQQTIPTTQKGLKLLAAIENKGTMPLNQLVVEA
                     RLSSQKGEFSAQDKVQVDRLSPGETRVVEFAGVAPAQMLPRSPAYRIKVTVESQQSGS
                     GLRSASREVMVRVVDSGQQEE"
     gene            1726..>2114
                     /locus_tag="VHS06_08245"
     CDS             1726..>2114
                     /locus_tag="VHS06_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988145.1"
                     /translation="MADRAAPTHIHGLPPSVPGLAVEKVKTSLHKPFDIPSRYRWLTL
                     VGIVLGLFISIEWQAPVAKAPVTSEYPRELSRETTRRLEAEQKDLKQTIAEKRADLAL
                     LQKDAAGRASTLAGLNTELESQRMLAG"
BASE COUNT          398 a          641 c          651 g          424 t
ORIGIN      
        1 gcggcctcat cggagccacg atgttcacga gggaatcgaa gctgcccagc gcttgctccg
       61 gcgccgaggc gggcacgtac agcgcagtca ggtggtccac ggacggctgg cgatcgaggt
      121 cgtgcaaatg cacctggtgc acagcctgtt cacccggcac ccccgcggcc gtcaccagca
      181 cgacagggtg atcgtccggg tatttgcgga gcaactccag cttgacgtcg gatgccacca
      241 gtcgatcctc tatctggtac accatgaggt cgatcaatgg gtttgcggag gccacgtgcg
      301 ctgcatccag caactgcaat cccccactga gggtggtgcg cgcggcagcc aacatcacct
      361 cgatgaagga cacccctgag acgatctgaa tctcgatacc ctcatctccg gccgctttga
      421 ggatctcacg gactgagtcc tcggccacgg tggggtctcc cggcactgcg tacacgatgt
      481 cgccctcgcg ccgtgcgatg gagattacct ccgcagcgag ttctcggtag agacagtcga
      541 agctctcaca gcggtcgtag acgtagtcca gcgactccca cttcaccgtc ttgggtagct
      601 tttcgactgt aggatgtcgt gaggttcgca gccacagctt gggtgtctga tccagcacct
      661 ctctggcttc ttgcgtcaaa agggggtatg gtcccggccc aagccccagg attgtgatct
      721 tcactgcgga taccgctcct tgttctcgat tcgcccaggg gctgcattgc cggatgggcg
      781 tcactgcggc tatggtaaca tagcagcctt cgagccgtgg ctcatcttca taatcccttg
      841 acaatatgtg gatgcctgac tatcatggcc accaaacctc ccctttccca cagcggggcg
      901 ggttagctag ccaggccgga gacttcctcc cctcgggacc cggccacatc aggagggacg
      961 catgcccggt aacaggggtg cggcacgtgt agtcgctttt cttcttgccc tctctgctct
     1021 tgccctgatt ctcagtgggt gtgagttggt tgttgtggag tcgagcggag ttcgtggcga
     1081 cgcccagcgg aacgctatgt cgacggcggt tgccgatctc cccgactacg acattgcggt
     1141 gagcgccatc gatttcgacc caccattgaa gcagcagacg ataccgacca cgcaaaaggg
     1201 tttgaagctg ctcgcagcta tcgagaacaa gggcacgatg ccgttgaatc aactggtcgt
     1261 ggaagcccgg ctgtccagcc agaagggcga gttctccgcc caggacaagg tccaggtcga
     1321 tcgactctcg ccgggtgaga ccagggtagt cgagtttgcc ggcgtagcgc cggcccagat
     1381 gctgcccaga tccccggctt accgcatcaa ggtgacggtg gagagccagc agtcgggcag
     1441 cggtctccgc tccgcctccc gtgaggtgat ggttcgggtg gtggactccg gacagcagga
     1501 agagtagtgc cccgtcgctt cttgaagttg gggtgcacgg gggagtgtcg agagttgatg
     1561 gggcgacgag cgtcgcccct ttttcgtggc cagaagatcg gtgagatcga ccctcgaggc
     1621 gtcgtatcac cagtagtgaa tatcgctcgt cggttagctt ctcttttgcc gcttcaactg
     1681 tagtgcgctc tggagtataa tcgcgctgca cagagaggaa gcagcatggc agatagagcc
     1741 gccccaaccc acatccacgg cctcccgcct tccgtgccgg gattggcggt ggagaaggtc
     1801 aagacctccc tccataaacc cttcgacatc ccttccaggt atcgctggct gaccctggtg
     1861 ggtatagttc tgggactgtt catctccatc gagtggcagg cgccggtggc caaggctccc
     1921 gtgacctccg agtatcctcg ggaactgagc cgtgagacca ccagacgact ggaggccgag
     1981 cagaaggatc tgaagcagac aattgccgag aaacgtgccg acttggcgct gctgcagaag
     2041 gatgccgcgg gacgcgcctc cacgctcgcc ggcctcaaca cggagttgga gtcacagcga
     2101 atgctggcag gcat
//
LOCUS       DATALS010000368         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_174422, whole genome shotgun sequence.
ACCESSION   DATALS010000368 DATALS010000000
VERSION     DATALS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174422"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VHS06_08250"
     CDS             <1..570
                     /locus_tag="VHS06_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988146.1"
                     /translation="RLELYRHSLQEGPAAAEERLQEVQALAQKGVVELRRMSNNLRPS
                     VLEDLGLVPALQFVVRELAQQLPGARTNFEVVGQEVRLPLEMELAVFRIAQEALSNVR
                     KHASSARRVSVALVFDDREVTLVVEDDGGGFQLPPSGALLQQGHLGLTGMAERARLFE
                     GELRVETAPENGTTVILRLPRPLPSPTAN"
     gene            736..>1800
                     /locus_tag="VHS06_08255"
     CDS             736..>1800
                     /locus_tag="VHS06_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988147.1"
                     /translation="MMAIVRTIPALMLMLAVLLPSARAASAAEESSFRLGFKALADQI
                     SEVAGQPLENEHYAANGDSLQKTTTGLMVWRKADNWTAFTDGNTTWINGPEGMQSRSN
                     GERFGWELATPERVLGIDPQQVRLDTRGLPFPSYRVDRVGASAYDVSTPPGPKGLPEH
                     LSVGFGLIDQPGPRAYLPVMYIVPTESYAQLWEQNGNRSVTKAISRIQELAQQIPSPT
                     PIRGMPLLPYELTTGVNDLAVQIKPVIGGENSALGTGFRYVGRFAQDANPVTDNGRLC
                     YIYQGFTRDGRYLVSFSCDVYTTSLPEHGQVPQAVQEKFASDYRGYMQETAAMLNSLP
                     GSAWEPDLEKLDAFVTSLRLV"
BASE COUNT          360 a          539 c          564 g          337 t
ORIGIN      
        1 cggctcgaac tgtaccgcca ctcccttcag gagggcccag ccgcggcgga agagcggctg
       61 caggaggtgc aggcgctggc ccagaagggc gtcgtggagc tgcgacggat gagcaacaac
      121 cttcggccat cggttctgga ggaccttggt cttgttccag ccctccagtt tgtggtgagg
      181 gagctggcgc agcaactgcc gggagccagg accaacttcg aggttgtggg gcaggaggtg
      241 cgcctgccgc tggagatgga gttggccgtc tttcgcattg cccaggaggc gttgtccaac
      301 gtccgcaagc acgcctcctc tgccaggcgg gtgagcgtgg cactggtatt cgatgatcgg
      361 gaggtgacgc tagttgtgga agacgacgga ggtggcttcc agttgccccc gtccggtgcc
      421 ttgctccagc aggggcatct cggcctcaca ggaatggccg agcgggcacg ccttttcgag
      481 ggggaactta gagtggagac ggctcctgag aacggcacca ccgtgatcct acgattgccc
      541 aggccgttgc cttcgccgac ggccaactga gcttacctca acgattgcca actacctcat
      601 tccaggtatc ctctaactga ctcgggtaat cgtggaaata cctcatcgct ctgatgtgcg
      661 cccctgccct gttgtccata atcacgctca agacaagccg aacacgggaa gagacaacag
      721 gaggcaggat aagagatgat ggcgattgta cgcacgatac cggcactgat gctgatgttg
      781 gctgtgctgc ttccaagcgc gagggctgcg agcgcagcag aggagtcatc gttcagactc
      841 ggcttcaagg cgcttgctga ccagatctcc gaggtggccg gccaacctct ggagaacgag
      901 cactatgccg cgaacggaga ctcactacag aagaccacta ccgggctgat ggtgtggcgc
      961 aaggccgaca actggacggc ttttacagat ggcaacacca cctggatcaa cgggccggaa
     1021 ggcatgcaat ctcgctccaa tggcgagagg ttcggctggg aactagcgac accggagcgc
     1081 gttctaggca tcgatcccca gcaagtgagg ctggatacca gggggttgcc ctttccctcg
     1141 taccgcgtgg atcgcgttgg cgcaagcgca tatgatgtga gcactccgcc cggcccgaag
     1201 gggctgcccg agcacctgag tgtcggcttc gggctgatcg atcaaccggg ccccagggcc
     1261 tacctccccg tcatgtatat cgtcccgacc gagtcctacg cacagttgtg ggagcagaac
     1321 ggaaaccgct ctgtcacgaa ggccatatcc aggatccagg aactggcgca acagatcccc
     1381 tctcccaccc ccattcgggg catgcccttg ctgccctacg aactgaccac aggggtgaac
     1441 gatctggccg ttcagatcaa accagtcatc ggcggcgaga acagtgcgct cggcacgggg
     1501 ttccgctacg tcggtcgatt cgcgcaggac gccaatccgg tgaccgacaa tggtaggctg
     1561 tgctacatct accagggctt cactcgggac ggcaggtatc tcgtgtcctt ctcctgcgat
     1621 gtctacacca cctcgctgcc agagcacggc caagtgccgc aagctgtgca ggagaagttc
     1681 gcgagcgact accggggtta catgcaggag accgccgcga tgctcaacag tcttccaggc
     1741 tctgcctggg agcctgacct ggaaaagctg gatgctttcg tcacttcgtt gcggttggtt
//
LOCUS       DATALS010000369         2709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_289009, whole genome shotgun sequence.
ACCESSION   DATALS010000369 DATALS010000000
VERSION     DATALS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289009"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VHS06_08260"
     CDS             complement(<1..604)
                     /locus_tag="VHS06_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2988148.1"
                     /translation="MASLEELQARLTRLDDIKRIEELQRIYGYYQDYGEWQKIVDLFT
                     DDDPSVEEADRGVYRGKESLSRYFVDLIGGGPEKPQRPGWLSIMFQLQGVISVEPGGT
                     TASGRWYGMGMEAKPIASLHEGELRQTWIHGIYENTYVKEDGVWKFKKLHFNLVFRTP
                     FEDGWLKVPVVGQNGPDKNIPPDAEPTAYHPYPSGYHLPIS"
     gene            758..1282
                     /locus_tag="VHS06_08265"
                     /pseudo
     CDS             758..1282
                     /locus_tag="VHS06_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
     gene            complement(1370..1522)
                     /locus_tag="VHS06_08270"
     CDS             complement(1370..1522)
                     /locus_tag="VHS06_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988149.1"
                     /translation="MVAVGAAVVVVVVPPHAAREASRDTRSNNAIKMNTDLFTVYSFL
                     STLEIQ"
     gene            1606..2529
                     /gene="dctP"
                     /locus_tag="VHS06_08275"
     CDS             1606..2529
                     /gene="dctP"
                     /locus_tag="VHS06_08275"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX2988150.1"
                     /translation="MAVPWPTGDPVTNNIQEFVDKFNAQAKGQYVIELHPGGQLLALP
                     DEFEALRTGGVEMAGWPVAVFGSIVPEFNLAELPFAVNSIEADAAYNVAMTPIYDKVL
                     TAQHNIKPVFNFTCQGLDIISVNPVKTLADWKGWLCQTISPVTANVVEKLGGAGVALD
                     FSEGYQALQKKVITAALESGSMIIMFKMNEVAKNLTRAYLTPAAIGIFINQDIYNKMP
                     KDIQDLVVNLGKEAQASTNANMIDLYHTNYDKMTGMGMTVYALPKAERDTWATQLQPY
                     ADELLGKVDPATATQVKDITKQLDQKYPYSE"
BASE COUNT          567 a          846 c          815 g          481 t
ORIGIN      
        1 aggagatggg taggtgatag ccggagggat aggggtggta ggccgtcggc tcggcatccg
       61 gcgggatgtt cttgtccggt ccgttctgcc ccaccactgg gaccttcagc cacccgtcct
      121 cgaacggggt acggaacacc aggttgaaat ggagcttctt gaacttccac accccgtctt
      181 ctttgacgta ggtgttctca tagatgccgt ggatccacgt ctgccgcagc tcgccctcgt
      241 gtagcgaggc gatgggcttg gcttccatgc ccatgccata ccaccggccg gaggccgtgg
      301 tccccccggg ctcgacgctg atgaccccct gaagctggaa catgatcgag agccaccccg
      361 gcctctgtgg cttctcaggg ccgcccccga tgagatcgac gaagtagcgg ctgaggctct
      421 ccttgcccct gtacacgccc cggtcggctt cctcgacgct cggatcgtcg tcggtgaaga
      481 ggtcgacgat cttctgccac tcaccgtagt cctggtagta gccatagatc ctctggagct
      541 cctcgatcct cttgatgtca tccaagcggg tcaaccgggc ttgaagttcc tcgagcgacg
      601 ccattccctc ccggctatca cctgcccatc tcgttcaagc atccggtgac cggggaatga
      661 ggggcggctg gtcatgacac tggaagagct cgagcgcgaa gtcacccggc tggaagacat
      721 ccagacgatc aaagacctct agtgcaagta cggctactac atggactccc atcatcgcga
      781 cgaggtattc gctctcttct ccgacgacac ggagtccata gagatcgaaa gcaccgggct
      841 ctttctcggc aaggagggag tgaggaagtt cttcatcgac aacgacctgc tggagccggg
      901 gcagacggag gtgccaagct ggatcaacat cctgatcatg atggacggcg cggtcatcga
      961 catcgacccc tcggggacag cggcgaaggg acggtggacc acctggctcg ccgaagcgat
     1021 gagcgtgggc ggcatcccgc gccagcagtg ggtccagggc tactacgaga acgagtacgt
     1081 gaaggaagac ggcaggtggc tgttcaagaa gctccactgg aacgtcacgt tcttcacgtc
     1141 tttcgagcgc ggctggctca acgtgccgct gctgggaagg ctggacaggc cggacgcgga
     1201 cgctcccgcg ccccacttcc atccctatcc ttcgggctac aggttgccct acagcttcgc
     1261 ccacccggtg acgggtgagt agctgagacg ggcaaaggcg acagggtttc gacgggcacg
     1321 aacattctgg taaaggaggt cttcactcag gaagcgatgg tagacccagc tactggatct
     1381 cgagagtgct gaggaaggag tagaccgtga aaaggtccgt attcatcttg attgcgttgt
     1441 tgctcctcgt gtctctacta gcttctctgg ccgcgtgcgg cggcacgacg accaccacta
     1501 ccgcggctcc gaccgccacc acggcggccc ccgagcggga ccaccgcagg gtccaccgcc
     1561 acaagcacgg gccaggtcga cacgtcgaaa gccaaggtgt tgcgattggc ggtaccgtgg
     1621 cccacaggcg acccggtgac caacaacatc caggagttcg tcgataagtt caacgcgcag
     1681 gccaagggcc agtacgtcat cgagctacat ccgggcggac agttgctcgc tctgcccgac
     1741 gaatttgagg ccctccggac aggcggcgtc gaaatggccg gttggcccgt tgccgtgttc
     1801 ggcagcatcg tccccgagtt caacctggcc gagctgccct tcgcagtcaa cagcatcgag
     1861 gccgatgccg cctacaacgt ggcgatgacc ccgatctacg acaaggtcct gaccgcgcag
     1921 cacaacatca agccggtctt caacttcacc tgccagggtc tcgacatcat cagcgtgaat
     1981 ccggtgaaga cgctggccga ctggaaaggg tggctctgcc agaccatctc cccggtgacg
     2041 gccaacgtgg tggagaaact tggcggcgcg ggggtggccc tcgacttctc cgaaggctac
     2101 caggcgctcc agaagaaggt catcaccgcc gccctcgagt ccggctcgat gatcatcatg
     2161 ttcaagatga acgaagtcgc gaagaacctc accagggcct acctgacgcc ggcggccatc
     2221 ggcatcttca tcaaccagga catctacaac aagatgccca aggacatcca ggacctcgtc
     2281 gtgaatctgg ggaaagaggc gcaggccagc accaatgcca acatgatcga cctctaccac
     2341 acgaactacg acaagatgac cggcatgggc atgaccgtct acgccctgcc caaggccgaa
     2401 cgcgatacct gggcgacgca gctgcagccc tacgctgacg agttactcgg caaggtagac
     2461 ccggctaccg cgacccaggt gaaagacatc accaaacagc tcgaccagaa atacccctac
     2521 tcggaataag cggacacgcc gtcctgcagc ccgctcgaaa gagcgggctg cagggtggag
     2581 gagtgactcg tgaagagatt cttgctcaag gcagacgctt cgatcggaaa ggtcagcgaa
     2641 tgggcgctga ttcttagcgg tgtgctgatc ctgctgatcg gcttcctcac cagctacggc
     2701 gtcgggaag
//
LOCUS       DATALS010000370         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_40624,
            whole genome shotgun sequence.
ACCESSION   DATALS010000370 DATALS010000000
VERSION     DATALS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40624"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="VHS06_08280"
     CDS             <1..1757
                     /locus_tag="VHS06_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX2988151.1"
                     /translation="YGSGQTLEFWLEDGMPVWRFRIGRTVVEKRVLMPHMQNTVYLSY
                     RLISGQGSIQLELMPAVHFRRLEDPVGPQQLQQYRLVTDGDRHELFGSSEYPPLRMMA
                     FGSEGKFVAKGSQLSEVLYRAEESRGYEYRGDLWSPGHFQLQLSRGHDATLVASTEDW
                     DVICALTPAEALFAEQERRRRLLQRAHPKARTGMAKELVMAADQFLIIPAGRVQDAAR
                     SHAAGDEVRTVIAGYHWFTDWGRDTMISLEGLALTTGRELEAGYILRTFAHYLQDGLI
                     PNMFPDGELKGRYNTSDATLWYFHAIDRYLEATGDRETLRILLPKLIGVVDCHLQGTR
                     FGIKVDPEDGLLCQGAEGLPLTWMDAHVDDWVVTPRRGKAVEINALWYNALRLLERWV
                     REERDEEEAQRLADYAALARSSFNRRFWYAEGGYLYDVVDGEKGDDPSCRPNQVLAIS
                     LRHPVLDRARWESVLQVALERLVTPVGLRSLAPSDPEYKPMYFGDRRSRDAAYHQGTV
                     WAWLIGPFIDAWLKLYPHDRTGARHFLEGFDAHLERACVGSISEIFDAKRPFIPRGCI
                     AQAWSVAEVLRCLVKTAE"
     gene            complement(2095..2156)
                     /locus_tag="VHS06_08285"
                     /pseudo
     CDS             complement(2095..2156)
                     /locus_tag="VHS06_08285"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          476 a          779 c          833 g          464 t
ORIGIN      
        1 aatatgggag cgggcaaact cttgagttct ggctggagga cgggatgcct gtgtggcgat
       61 tccgcatcgg ccggactgta gtggagaagc gggttctcat gccgcacatg cagaacaccg
      121 tgtacctttc ctatcggctg atttctgggc agggctcaat ccagttggag ttgatgccgg
      181 cggtacactt caggcgcctc gaggatcccg tcggccctca acagctgcag cagtaccgtc
      241 tcgtgactga tggcgaccga cacgagctgt tcgggagcag cgaataccct cccctcagga
      301 tgatggcctt cggatccgag gggaaattcg tggcgaaggg cagccagttg tcggaggtgc
      361 tctaccgcgc tgaggagagc agggggtacg agtatcgagg cgacctgtgg agccccggcc
      421 acttccagct gcagctttcc aggggacacg atgcgacgct cgtcgcctcc accgaggatt
      481 gggacgtaat ctgcgccctt actccagccg aggcgctgtt cgcggagcaa gagcgaaggc
      541 gcaggctgct gcagagggct caccccaagg cccggacggg catggcgaag gagttggtga
      601 tggccgccga ccagttcctg atcatcccgg ccgggcgcgt ccaggatgcg gcccgctccc
      661 acgcggcagg ggacgaggtg cggactgtga tcgcagggta ccactggttt accgactggg
      721 gtcgagacac catgatcagc ctggagggcc tggcgctgac gacgggacgg gaactggagg
      781 ccggctacat ccttcggacc ttcgcccatt acttgcagga cgggttgatc ccgaacatgt
      841 tccccgacgg tgaactgaag ggacgctaca acacctccga cgcgaccctc tggtatttcc
      901 acgccatcga ccgctacctt gaggcgacgg gcgacaggga gacgctgagg atccttctcc
      961 ccaagttgat aggggttgtg gattgccacc tgcaggggac gagattcggg atcaaggtgg
     1021 atccggagga tgggttgctg tgccagggcg cggaggggct gcccctcacc tggatggacg
     1081 cacacgtaga cgactgggtt gtgacacctc gcaggggcaa ggcggtggag atcaatgccc
     1141 tctggtacaa cgccctccgt cttctggaga ggtgggtgag ggaagagcgg gacgaggagg
     1201 aggcgcagcg actcgccgac tacgccgcac tggccagatc ctcgttcaac cgacgcttct
     1261 ggtatgcgga agggggctac ctgtatgacg tggtggatgg cgagaagggt gacgatccct
     1321 cctgccggcc caaccaggtc cttgccattt cgttgcgtca tccggtcctc gaccgcgcca
     1381 ggtgggagtc ggttcttcag gttgcgttgg agcggttggt cacgccggtg gggctgcgct
     1441 cgcttgcgcc gagcgatccg gagtacaagc cgatgtactt cggggaccgc cgatccaggg
     1501 atgccgccta ccatcaggga acggtgtggg cgtggctcat tggtcccttc atagatgcct
     1561 ggctgaagct gtaccctcac gaccgcacgg gggcgaggca cttcctcgag gggttcgacg
     1621 cccacctgga gcgtgcttgt gtcggatcca tcagcgagat tttcgatgcc aagcgcccct
     1681 tcatcccacg gggctgtatc gcccaggcat ggagcgtggc cgaggtgctg cgctgtctgg
     1741 tgaagacggc agagtgactc gcggacgaag tcctgggagc gcgggcgtct cgcccgcatc
     1801 atcgtggccc ggtcgacgtt tcggagtcga ctcacgattc ccccgtcaag gccggcgccc
     1861 aaccagtgtg gcaggtagac cccaaggttc tccgccagca acagagtgcc caacaccacc
     1921 agggttgccc cagcatagaa gccaaatgcg gtcccctcac caaaccttcg gccccagcgc
     1981 cgcgtccgct cggacatctc tctgccgccc tgaccatacg tcgcttcggt gacatccgcc
     2041 ctccgtgccg ctatccagag agccagatat actgctaccc cgaggccgtt gaatattgcc
     2101 cgaagcaaga aggtgaggcg gaccagcgtg gtgtctacgc ccaggtagtg cgccaccccc
     2161 ccccgcagac gcccgcgatc atcgagtctg ttctgctccg gtacagcctg gcccgcattg
     2221 tcctaaacct ccctcaccca cgatcggcat cgcaacctgt cggatggttt gcccaacgtt
     2281 ctcttggact gcaggctaat ccaaaaggtt tcctgtcgga tcgaacaaag tggggattcg
     2341 gcaatacgcg gaaaggcgtg ccagaggaag acgtcggcta caactaatgt cgtagcctga
     2401 aatggagttg ggcagatgag cgccgccttg acgggggaat cgtgagtcga ctccgaaacg
     2461 tcgaccgggc cacgatgatg cgggcgagac gcccgcgctc ccaggacttc gtccgcgagt
     2521 cactctgccg tcttcaccag acagcgcagc ac
//
LOCUS       DATALS010000371         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_396887, whole genome shotgun sequence.
ACCESSION   DATALS010000371 DATALS010000000
VERSION     DATALS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396887"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VHS06_08290"
     CDS             <1..343
                     /locus_tag="VHS06_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988152.1"
                     /translation="TASLHKMSALRRDLEAWHGRHLSREEEKSFDLRSLLGKPAMVTV
                     TETENGKRKISGISGLAKGMPVPKPVNPPLYFSFDPAEFSEEALEQVPQFWRDVIRQS
                     PEYKLLQGATR"
     gene            486..>2458
                     /locus_tag="VHS06_08295"
     CDS             486..>2458
                     /locus_tag="VHS06_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEX2988153.1"
                     /translation="MQLADLDRLKHEVRIDEQLARRGISPVQKYGNRLTYHCPIPGHD
                     DRNASCSVYLDENRFHCFGCGSSGSVVDMVAVLEGRDPKDVIRDWLNRGDRIPTPAKE
                     HATLQQFQREESTGSRATRPCTVAALAERCRLPIEHLRSFGLSDVNYGGSPAVKIPYV
                     DTEGNEAAVQFRLALEKTEESKRFAWRKGSRPTLLGWPLLAEAAKLGYVVIPEGASDF
                     LTLRFHGFATIALPASTGWKEEYAAAFDEVERIYVVIEPDQGGRTVRDSFRASSLSDR
                     VNLIYMSEECPDPSELHKQDPNGFKARFEKLIEESIPLTVEVRQEASARADRAWQQCL
                     ELAPSPRILDRFVTSLHRRGVAGEDRLAEILFLALVSRHLDRPVSVAVKGPSSGGKSY
                     LIEQVLSYFPPSAYFSLSGMSEHALAYMEEPLAHRHLVVYEAAGMEGELATYLIRSLL
                     SEGVVRYATVVKTGDVPRQKVLELEGPTGLIVTTTRASLHPENETRLLSLTVTDTPEQ
                     TRSVFRAIADESERSQDQTELEPWIALQTWLDLAGQHKVAVPFALDLAELIPPVAVRL
                     RRDVSTVLNLVRAHALLHQARRERDQTGRVVATLDDYAVVRDLVSDLLSSEVGLAVSH
                     ETREAVQAVRELNNQTGKPISNRQVAEHLHLDAS"
BASE COUNT          521 a          746 c          742 g          449 t
ORIGIN      
        1 cacggcatcc cttcacaaga tgtcggcact caggcgagac ctcgaagctt ggcacgggcg
       61 ccacctcagc agggaagagg agaagtcgtt cgaccttcgc tccctgctgg gcaaaccggc
      121 aatggtcact gttaccgaga ccgagaacgg caaacggaag atcagcggta tctcaggtct
      181 ggccaagggt atgccggtgc ccaaacccgt caatccacca ctctattttt ccttcgatcc
      241 ggctgagttc agcgaggaag ctcttgaaca agtgcctcag ttctggaggg acgttatccg
      301 gcaaagcccg gagtacaagc tcctccaggg ggcgacgagg tgagcggccc cggcggcgct
      361 gatcgcggcg tgcggcgacg tcacgccgtc cgaccggaca atcttctacc tgttgaagca
      421 ggcgtgccca cctccaccat tcggtggggg tgggccgaca acaatactaa tccggacgag
      481 tgcacatgca acttgctgat cttgaccgac tgaagcacga agttcgcatc gacgaacaac
      541 tggcccgccg cggcatctca ccagtgcaga agtacggcaa ccgattgacc taccactgtc
      601 ccattccggg ccacgacgat cgcaacgcga gctgcagcgt atatctcgac gagaaccgct
      661 tccactgctt cggctgcggc tccagcgggt ccgtcgttga tatggtcgcc gtcctcgagg
      721 gccgggatcc gaaagatgtc atccgggact ggctcaaccg gggggacagg atccccaccc
      781 ccgcaaaaga gcacgcaaca ctgcaacagt ttcagcgcga ggaatccacc gggtcccgcg
      841 cgacaaggcc gtgcaccgta gcagcgctgg cggaacggtg ccgccttccg atcgaacacc
      901 tgaggtcctt cgggctctcc gacgtaaatt acggtgggag cccggccgtg aagattccct
      961 acgtcgatac ggaagggaac gaggcggcgg ttcagttcag actcgctctc gaaaagaccg
     1021 aagaatcaaa gcgcttcgcc tggaggaagg gatcaagacc cacactactc ggctggccgt
     1081 tactagccga ggcggcgaag ctcggctacg tcgtcatccc ggaaggagca tccgacttcc
     1141 tgaccctgcg tttccatgga tttgccacga tcgctttgcc cgcatcaacg ggctggaagg
     1201 aggagtatgc ggctgcgttc gacgaagtgg agagaatcta cgttgtaata gagcccgacc
     1261 agggcgggcg aacggttcgg gattcgtttc gagcgtcgag cctctcagac cgggtgaatc
     1321 ttatctacat gagcgaagaa tgtccggacc ccagcgagtt gcacaaacag gatccgaacg
     1381 ggttcaaggc tcgcttcgag aaactaattg aggagagtat ccccctgacc gtcgaggtgc
     1441 ggcaggaagc atctgctagg gcagacaggg catggcagca gtgcctcgag cttgcgccct
     1501 cccctcgaat cctcgaccgg ttcgtaacgt cgctgcaccg tcgaggcgtg gccggcgaag
     1561 accgtcttgc tgaaatcctc ttcctagccc tcgtcagtcg tcatctggac cggcctgttt
     1621 ccgttgcggt caaaggaccg tcgagcgggg gcaagtcgta cctcatcgag caggtcctat
     1681 cctactttcc gccctccgcc tacttcagtc tgtcaggcat gagcgagcac gcgctcgcct
     1741 atatggaaga gccgctcgcc catcgccatc tagtcgtgta tgaggcggcc ggcatggaag
     1801 gggagctcgc tacctacctg atccgaagct tgctttcgga gggtgtcgtc cgctacgcga
     1861 ccgtggtcaa gaccggtgac gttccacgac agaaggttct agaactggag ggtcccaccg
     1921 gactaatcgt cacgacaact agagcaagtc ttcatccgga aaacgaaacc cgtctgctgt
     1981 cgctgacggt gaccgacacg cccgagcaga cgcggtcggt ctttcgggca atcgccgacg
     2041 aatcggagag gagccaggat cagacggagc tagagccgtg gatagctctt cagacttggc
     2101 ttgacctggc cgggcagcac aaggtggcgg ttccattcgc gctggatctg gccgagctca
     2161 taccacctgt cgcagttcgg ctgcggcgag acgtctctac ggtgctcaat ctagtaagag
     2221 cccatgcgct acttcatcag gcgaggcgag aacgggacca gacggggcgg gtggtcgcga
     2281 ccctcgatga ctacgccgtt gtccgcgacc tcgtgtcgga tctcctgtca tcagaggtgg
     2341 gcttggctgt gtctcatgag acccgtgagg cagttcaggc cgtccgggaa ctgaacaacc
     2401 aaactggaaa accgatcagc aacaggcaag tggccgagca cctgcatttg gacgcctc
//
LOCUS       DATALS010000372         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_289071, whole genome shotgun sequence.
ACCESSION   DATALS010000372 DATALS010000000
VERSION     DATALS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289071"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VHS06_08300"
     CDS             complement(<1..459)
                     /locus_tag="VHS06_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2988154.1"
                     /translation="MVRGARSSLIPPYSVIACSIVFFWSISVRQLICTVLPSMAADID
                     VSNSAAGILVSTMLLGYTAGSWLSGWLPGSRKLRILAGVLLSIPSSVFFSFAPTYSLL
                     LLSGVLLGFGVGIYLPLGLSLMVEVGDATSRKGYYLALHEASATFATFGGS"
     gene            complement(555..1151)
                     /gene="hisB"
                     /locus_tag="VHS06_08305"
     CDS             complement(555..1151)
                     /gene="hisB"
                     /locus_tag="VHS06_08305"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX2988155.1"
                     /translation="MDVIKRQGSFSRKTAETDISVSWVVDGAGECHSDTGIGFLDHMI
                     AQLAKHGQFDLDVRAQGDLQVDEHHTLEDVAIALGRALDVALGDRRGLSRMGDALVPM
                     DESLARVAVDLGGRGMAVVGAQFAQGAGGFHGDMVAHFMESFAREGRLTLHVDVLRGD
                     NDHHKIEAIFKSLARALDAACRLDPRRAGSMPSTKGVL"
     gene            complement(1271..>2319)
                     /gene="hisC"
                     /locus_tag="VHS06_08310"
     CDS             complement(1271..>2319)
                     /gene="hisC"
                     /locus_tag="VHS06_08310"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX2988156.1"
                     /translation="EMEEYIPISTPEALAEKLGLRPDQIIKLDGNENPYGCSPRVPKA
                     LAEFASYHRYPDPMQSEARSMLARYVGVPADYLMFGMGSDELIDDLLRVYLEPGDEVI
                     ECPPTFGMYSFSTQICAGRIVRVPRGEQFQIDLEAVERAITPQTRVIFVDSPNNPSGL
                     VTSREQVERLLAAGRLVVVDEAYAEFASFTATNMVLDHPNLVVLRTFSKWAGLAGLRA
                     GYGVFPQAVIRQLWKMKQPYNLNLAAQIAVQESLMDVDLLMSNVRKIVDERERLLRTL
                     STVEYLRVHPSEANFLLCDLLEGDAVELVGFLARRGIIIRYFKKPRVSNSIRISIGRP
                     EQNDAVVAALREWR"
BASE COUNT          489 a          761 c          682 g          387 t
ORIGIN      
        1 ggaaccaccg aaggtcgcga aggtggccga cgcctcgtgg agcgccagat agtacccctt
       61 ccggctcgtc gcatcgccca cctccaccat gagggagagg cccaggggga ggtatatacc
      121 gaccccgaac ccgagcaaca caccggatag gagcagcagc gagtaggtgg gcgcgaagga
      181 aaagaagacc gaggaaggta tggatagcag cactccagcc aggatgcgaa gcttccggct
      241 gccgggcaac catccggaca accaactgcc ggccgtgtag ccaagcaaca tggtggaaac
      301 caggatgccc gcggccgaat tggagacgtc gatgtccgcg gccatgctcg gcagcacggt
      361 acagatcaac tgccgcacgg agatggacca gaagaagacg atggagcaag cgatgacgga
      421 gtagggcgga atcaacgagg agcgtgcgcc ccgaaccata acgggggaac tctcggcacc
      481 atggctctca tcccgttgac atgccctctg gcatgtcaac gcctctccct gagggagggg
      541 atcgatatcc gacactagag cacgcccttg gtactgggca tagagccggc gcggcgtggg
      601 tcaagacggc aagcggcgtc cagggcacgg gccaacgact tgaagatggc ctcgatcttg
      661 tgatgatcgt tatcaccccg cagaacgtcg acatgcaggg tgaggcggcc ctctcgagcg
      721 aaggactcca tgaagtgtgc gaccatgtct ccatgaaagc cgcctgcccc ctgggcgaac
      781 tgcgcaccca ccacggccat tcccctgccg ccaaggtcca ccgcaacgcg agccagcgac
      841 tcatccatgg gaaccagggc atcacccatc cggctcagcc ccctgcgatc gccgagagcg
      901 acgtccaggg cacgcccgag ggcgatcgca acatcctcca gcgtgtggtg ctcgtctacc
      961 tgtaggtcgc cctgtgccct cacatccagg tcaaactgcc catgtttggc caactgggcg
     1021 atcatgtggt ccaggaaacc tatgccggta tcggagtgac actccccggc accgtccacc
     1081 acccaactta ccgagatatc ggtctccgcc gtctttctgg agaagctgcc ctgcctcttg
     1141 ataacgtcca tcgagcgact ccgaattcga gtgtagcgtc ggggcctgtc cccgacgtgg
     1201 gatcgttgcc acgggcacta cgtcgggcgt gaggctgtgc tcgctcgcct cacgagcccc
     1261 gacgctacag tcagcgccac tctcgcagtg cggccacaac cgcgtcgttc tgctccgggc
     1321 ggccgatgct gatccgaatc gagttggaca cccgcggctt cttgaagtag cgaataatga
     1381 tcccccggcg agcgaggaaa ccaaccaact ccaccgcatc gccctccagc aggtcacaca
     1441 gcaggaaatt ggcttcggaa gggtggaccc gcaggtactc gacagtcgag agggtcctca
     1501 gcaacctctc cctctcgtcc acgatcttcc ggacgttaga catcagcagg tccacgtcca
     1561 tcagcgactc ctgcacggca atctgcgccg ccaggttcaa gttgtaaggc tgcttcatct
     1621 tccacaactg ccggatcacg gcctgaggga agacgccata cccggcccgc aagcccgcca
     1681 ggccggccca cttgctgaaa gtccttaaca ccaccaggtt cggatggtcc agcaccatat
     1741 tcgtcgccgt gaaggaggcg aactctgcat atgcttcgtc caccaccacc aaccttcctg
     1801 cggccagcag ccgctccacc tgttcacgcg aggtcaccaa ccccgaggga ttgttaggag
     1861 agtcgacgaa gattacccgg gtctgagggg taatggcacg ctccaccgcc tccagatcga
     1921 tctggaactg ctcgcctcgg ggcactcgaa cgatgcgtcc ggcacagatc tgagtgctga
     1981 aggagtacat gccgaaggtt ggagggcact cgatcacctc gtcgcccggc tccaggtaga
     2041 cccgcagcag gtcgtctatc aactcgtcgg agcccatgcc gaacatcaga tagtccgccg
     2101 gcaccccgac gtagcgtgcg agcatagagc gagcttcgga ctgcatcggg tccgggtagc
     2161 ggtgatatga ggcgaactcg gccagcgctt tgggaacccg cggcgagcag ccgtaggggt
     2221 tctcgtttcc gtccagcttg atgatctgat ccggacgcaa tcccagcttc tcagccagcg
     2281 cttccggggt actgatgggg atgtactcct ccatctcct
//
LOCUS       DATALS010000373        11928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_289245, whole genome shotgun sequence.
ACCESSION   DATALS010000373 DATALS010000000
VERSION     DATALS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289245"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..498
                     /locus_tag="VHS06_08315"
     CDS             115..498
                     /locus_tag="VHS06_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988157.1"
                     /translation="MAERSEFFGPEPRGATATIGATGLATQWNLAEGSTQPPFQTSIS
                     VLNPSSSATAVRMDFQLENGQVVTRDFIAGAASKLTVNVGDFLPNDAFSTKVTTSQPT
                     VVERLMFLNKLGFLGETDALGTLPR"
     gene            702..824
                     /locus_tag="VHS06_08320"
     CDS             702..824
                     /locus_tag="VHS06_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988158.1"
                     /translation="MEDEVKDDDGLEESPRQMAARLLMSGTVLLVLLVLGQLLS"
     gene            complement(821..1315)
                     /locus_tag="VHS06_08325"
     CDS             complement(821..1315)
                     /locus_tag="VHS06_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX2988159.1"
                     /translation="MPSRTTRTPGQSAIKQPEVDEAYPQIPEPKQSHTGSLLAFVSVL
                     AVLGFGFWTALTTYMGPGDGKATAKPTATAAATARAATTPAAVSAATAVSSTPSAGSS
                     GTPAAGATATGASGSRVHVVGDGDTLYKIATRYGTTVDAIVAANGFPDRGKVLHVGDR
                     ITIP"
     gene            complement(1540..1674)
                     /locus_tag="VHS06_08330"
     CDS             complement(1540..1674)
                     /locus_tag="VHS06_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988160.1"
                     /translation="MEYYLVAMIAVLAFLGIMSRAIDRLQLTSEDCSEEPRDDMGEAA
                     "
     gene            complement(1863..2069)
                     /locus_tag="VHS06_08335"
     CDS             complement(1863..2069)
                     /locus_tag="VHS06_08335"
                     /inference="COORDINATES: protein motif:HMM:NF025436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HEX2988161.1"
                     /translation="MGVGNVSGAATAAVMSQLKGADVRSVVGLAVMRQIQDQQKQMGQ
                     EMLDMINSSTPSPNGVGQRIDIRV"
     gene            2336..2719
                     /locus_tag="VHS06_08340"
     CDS             2336..2719
                     /locus_tag="VHS06_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988162.1"
                     /translation="MAKGRVLAKRSDLANKSAFDRKLTYAAWALFFVAWGLSQWTEFS
                     SGISLRNLQYLGIGLILLGLNLARYLNRIYMSRLTLTLGLIATFGGLYRLLSGDESLI
                     PLIPIALLSPLVVEGLARWMGSAKG"
     gene            2824..3294
                     /locus_tag="VHS06_08345"
     CDS             2824..3294
                     /locus_tag="VHS06_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX2988163.1"
                     /translation="MKSSGNVTRQPQIGQSWFAMAVHILALLARSPEGYPSAYLAGSV
                     NTHAVFLRKVVAHLVRAGLVVAQEGREGGYRLARPADQISLADVYRVMYAGGPLPPNP
                     AEPNPLCEVGSGMRAALGEVASEVEQQMVRDLERHTIAEISDRAVLLGNFPNGR"
     gene            3497..4342
                     /locus_tag="VHS06_08350"
     CDS             3497..4342
                     /locus_tag="VHS06_08350"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2988164.1"
                     /translation="MKIAITGASGQLGHLVINKLKAKIPAANIVALARTASKAADLGV
                     TVREADYTRPQTLDHALVGVDTLLFISGSEVGQRVAQHHNVIEAAKRAGVKWVVYTSL
                     LRADTSPLSLADEHRATEAELKASDIPFTLLRNGWYTENYTGSVPGAIAGGAFLGCAD
                     DGKISSATRDDYAEAAVAVLTSEGHVGKTYELAGDDAYTLSDLAAEISRQTGKTIPYK
                     DLPEAEYAAALTGVGLPEGLARAFAGYDVAAARGALFDDGRQLSALIGRPSTPLSVAV
                     ADVLK"
     gene            4444..5442
                     /locus_tag="VHS06_08355"
     CDS             4444..5442
                     /locus_tag="VHS06_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010353809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEX2988165.1"
                     /translation="MSRQHEHEGPEADRGYRLLRLDDYRELVSLDALFKAGSPISPES
                     RNRVVDDLLETLAPEGASRRLGLRPEAIERLKRDPEQARRLLRAFLTVRGPEPLLESF
                     NLMLDGLLQLELQERGQVELPSLTRIRDLLPESSYPALPYCALWRGDITRLSVDAIVN
                     AANSRMLGCFVPFHACIDNAIHSTAGPRLREDCDRIMRLQGGPEPTGGAKATRGYNLP
                     ARFVLHTVGPIVEKELTEAHEKALASCYISCLDLASRLPDVHSVAFCAISTGVFGYPK
                     LPAALVALQTVSEWLRAHPGSLDLVVFNVFGDEDLAVYQVALEKWDSASCVRRTKQ"
     gene            5538..6317
                     /locus_tag="VHS06_08360"
     CDS             5538..6317
                     /locus_tag="VHS06_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase of SIR2
                     family"
                     /protein_id="HEX2988166.1"
                     /translation="MTASVGIDYTDPVSFARLFPALVRRGFKARYELIGYEDWAPEEK
                     WAYWATHVNDVRFGSRHDQVYRQLLDLVVGRDYFVVTSNVDALFARNGFEEKRLFTPQ
                     GDYALLQCRKPCTRRVWPFRPIMDQILPAIDPYSFRVRDSAVLPRCPDCGGEVFLNVR
                     VDGSFIDDPYVEQYHRLSHWLREAREGALLVVEIGAGFNTPSVVRWPIEGVVETTPGA
                     KLVRINLSQPEVPSEIADRSISVRSDAGEAIDAITRGLQRR"
     gene            6459..6857
                     /locus_tag="VHS06_08365"
     CDS             6459..6857
                     /locus_tag="VHS06_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988167.1"
                     /translation="MRCEAVVKRTGEQCGREASRGSRFCRYHRRYASGGTLPGDRPSF
                     YSDVMEEGALELEVASALHGVEDEIAVLRMLIRRVAREGDLEATRKGIETLCRALKVQ
                     YALDGRSADGLAGSLAKVLDEVGNELGMSL"
     gene            6868..7152
                     /locus_tag="VHS06_08370"
     CDS             6868..7152
                     /locus_tag="VHS06_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988168.1"
                     /translation="MAEDGSEALRSALKRFSPPSAPHIGGPDDRGIRELACRLDDRGC
                     AYGRISNARLEDLSRGYERVEAKLNAVLLAAVGTFVSSLVGMAVYHLRGG"
     gene            7155..8078
                     /locus_tag="VHS06_08375"
     CDS             7155..8078
                     /locus_tag="VHS06_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2988169.1"
                     /translation="MERCVELEASPEYQERQEREERITRLALRYRSGDRGVLGELYAA
                     VEPLIHHFLRIHLSPPRSLPCGVEPADLYQQAFVALADTTLEWDPERRDNFMPYFFHS
                     FPWRIDHYLRAQSPARRSSRFQLFSLPHDVLVERMDSMPGPDGRDWDGALACAELMGE
                     LPRQYAQVVRLHLFHGLPFAEVGAALGMGRSAAHESFGRAMALLRSRLDGAPRVSGVS
                     GGEGASHPPEVDSAVLRRCVEVMHRLAPGNSPLPGRTAICSAAGLTGREYLRIMERLC
                     ARGCVVGRRPGSPGSLAYAGPDETLRRLNGF"
     gene            8134..8904
                     /locus_tag="VHS06_08380"
     CDS             8134..8904
                     /locus_tag="VHS06_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2988170.1"
                     /translation="MDLTLTFLGTGTSHGVPAIGCRCPVCTSDDPRNNRYRCSILLQY
                     GGHNVVVDTATEFRLQAIRIGLDRLDAVLFTHSHADHIGGLDDLRSFSERQGAAIPCY
                     GSAECLDDIRHRFDYIFKETQLGGGKPRLDMHPVDGPFELFGLRIVPVPVYHGRLLVL
                     GFRFGRTAYVTDCNSIPEGSEEMLKGLDVLVLDALRWRSHPTHFNVEEALAEVAKLRP
                     RHTYFTHLTHDLDHATTNAQLPQGVELAYDGLTLALEV"
     gene            9244..10518
                     /locus_tag="VHS06_08385"
     CDS             9244..10518
                     /locus_tag="VHS06_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988171.1"
                     /translation="MNGRWVVKAALLFLLLLAPGAISAGTGALSGGLPGIPDRASTVP
                     SQPRPTATAEAIAPTPEPTPEPTPIPDPSLYRLPANWQKGVVLWDRPESFGSPASFQS
                     LRELRDVGATSISPVVTWYVAGPWDPHIHPGKKTPTDDDLIAVIDEAHNLGLQVMLRF
                     HIDCEDGNWRARIDPQDKDAFFNAYTTVLGYYAGLARDHAVEGIAIGAELSSLTKPAY
                     TDYWRSVIVQVRQRFPGFLTYSAQWGSAPPDALADPFREFEQIQFWDDLDYLGISGYF
                     ELVAAGQTPSLQSYIDMWSRWRRLRIEPFQAKYNKPLIFTEIGYASTPTAAIHPWAGG
                     GAGAIDLQLQAELYSALFESWADVPWFRGAYFWFWPTSPGGLGLEGADYPPSGKPAQE
                     VLRRWYRLLDDMEQPPWKPSSAGGTIPQEEGR"
     gene            10524..11498
                     /gene="corA"
                     /locus_tag="VHS06_08390"
     CDS             10524..11498
                     /gene="corA"
                     /locus_tag="VHS06_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX2988172.1"
                     /translation="MATKSTPVPGIIGPGPQGGGPNIQVVRHGRLTWVNLERPTKDEI
                     EWLRQNYSFHPLHLDDCLSKIQRPKIDEEKDYLFVVLHFPVFNRVAGVTVASEVDAFI
                     GADYFITVHSGDIKPLRRMFQECHNNPAYRQELMCRSSGYLLYKVIDRLVDYCFPILN
                     KIDANIEAVEDTIFEENVRQTVRDISVIRRDIINFRRIVKPQIAVIASLEHKERPYLQ
                     EDLEAYFGDIADHVAKIWDILEDYKEVIEGLSDTNDSLTSYRINEVMKILTTISVIML
                     PLTLVSGIYGMNIGLPLQEHSMAFLVVMGIMLAIATGMLSYFRKRGWI"
     gene            complement(11593..>11928)
                     /locus_tag="VHS06_08395"
     CDS             complement(11593..>11928)
                     /locus_tag="VHS06_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2988173.1"
                     /translation="RVLMARRKFEPFKGQWTLPSGFMEYGESPEETAVREMWEELGVE
                     VELTGLVSVEMERGDPRGLCLLVVYTGRIVDGEPKAGDDAAELKSFPLDQLPLEIAFD
                     AHRRALQKL"
BASE COUNT         2161 a         3643 c         3796 g         2328 t
ORIGIN      
        1 ggtttccccg accgaggcaa ggtccttcac gtcggcgacc gcatcaccat cccctagctg
       61 agcaacttcc ccaacacctc cttcggcatt caggtgaatg cggaccagcc gatcgtggcc
      121 gagcgctcgg agttcttcgg cccggagcca cgcggcgcga cggcgacgat aggagccacc
      181 ggcctggcga cgcagtggaa cctggcggag ggctcaaccc agcccccctt ccagacctcc
      241 atctcggtac tgaaccccag cagcagcgcg acggcggtca ggatggactt ccaactggag
      301 aacggacagg tagttacccg agacttcatc gcgggtgcgg ccagtaagct cacggtcaac
      361 gtgggcgact tcttgccgaa cgacgccttc tccaccaagg tcacgaccag ccagccgacg
      421 gtggttgagc ggctgatgtt cctgaacaag ctgggcttcc tcggggagac tgacgccctc
      481 gggaccctgc caaggtgagt gtgaccgggt accggcgtcg cgtcaacgac gcatcggtat
      541 tggtgtcatc ctgagcggga gcgaaggatc tctccagaca cggggagatc cttcacttcg
      601 ttcaggatga caaatctacc gtggtcgtgt actagccgtg tccctgctct ggtatgtcct
      661 ttgcttcccg cgggcctctc gatgaagaag gggaggatga cgtggaagac gaggtcaaag
      721 acgacgacgg gcttgaagag agcccgcgac agatggcggc gcggctgctg atgtcgggga
      781 ctgtgctgct ggtgctgctg gtgctggggc agttgctcag ctaggggatg gtgatgcggt
      841 cgccgacgtg aaggaccttg cctcggtcgg ggaaaccgtt ggcagccacg atggcatcga
      901 cggtcgtgcc gtagcgagtg gcgatcttgt acagcgtgtc gccatcgccg accacatgca
      961 ccctggaacc tgatgcaccg gtcgcggtgg cgccggcggc cggtgtgccg gacgagcctg
     1021 ccgacggggt agacgacacg gcagttgccg ccgacacggc ggcaggagtg gtagccgcac
     1081 gggcggtcgc cgccgcggtg gcagtgggct tggcggtggc tttgccatcc cccgggccca
     1141 tgtaggtggt cagagccgtc cagaagccaa atcccaacac tgccagcacg ctgacgaaag
     1201 ccagcagact gccggtgtgg ctttgcttcg gctcggggat ttgcgggtac gcttcgtcga
     1261 cctcgggctg cttgatggcc gattgcccgg gggtgcgagt cgttcgactg ggcatcgaaa
     1321 cctttgctct cgccggccag agagattttg ggctagttgg ccggctgaat cgtgggtgtg
     1381 gctccgtaga ccggcgccgg cagcatcata gggggatggc gagaatatgt caaacactcg
     1441 cggctttgtg gggtatctcc gcgaacagga cggtttcggg gaatggctgt tgacatcccc
     1501 ggccggcggg tgagcgtgcc gtcgatatcg gacgaaggct taggcggcct ctcccatgtc
     1561 gtcgcgtggc tcttccgagc agtcctcaga tgtgagctgc agccggtcaa ttgcgcggga
     1621 cattattccc aggaatgcca gaaccgcgat catcgccacc aaatagtact ccatcgtcgg
     1681 cgcttctcct ctcgagggag tgatcattgc ctatcgcggc ccgggcttcg ctgccgctcg
     1741 tcatgaacta ctgcaacttt cttgccatcc ttcaagaatc cgcgagcccc gccaacgcgg
     1801 ggctcgcgtc atttctgtac ggctttgcgc gctattaggg ctctatcacg aactgcagtg
     1861 tgttagaccc ggatgtcgat cctttggccg accccattgg gggacggcgt cgagctgttg
     1921 atcatgtcca acatctcctg gcccatctgc ttctgctggt cctggatctg tcgcatgacg
     1981 gcaagcccga cgacagagcg gacatccgca cccttcagtt gtgacatcac ggcagccgtg
     2041 gcggcacccg agacgttgcc cacgcccata gattcacctc catcggcggc agcgccgcgt
     2101 cgataggtgg cccttttgag gcttccagtc gctatgctag cgtggaacgg gcggacctgg
     2161 cagggccggt aggcaccaat agccgagggc cgatggtccc gcccgttctc cttgctccga
     2221 tttcgtatgc ttgacactcc ctatcctgca tcttatcctg ccttccgccg ggggtgccgc
     2281 ttcttcacct gccgcggggc tccctgctct ccacaatctt cagggagcgt gtgccgtggc
     2341 caaaggaaga gtccttgcta agaggtctga tctcgccaac aagtctgctt tcgataggaa
     2401 gcttacctat gccgcttggg ccctgttctt cgtcgcctgg ggactgtccc agtggacgga
     2461 attcagcagt gggatcagtc tgcggaatct ccagtacctc ggcattggcc tcatcctgct
     2521 ggggctgaac ctggcccggt atttgaatcg catctacatg agccggctta ccctcaccct
     2581 tggtctcata gccacattcg gtggccttta ccgcctgttg agtggggatg agtcattgat
     2641 cccgttgatc cccattgccc tgctgtcgcc cctggtggtg gaagggctgg cacgttggat
     2701 gggatcggca aagggctgag cagatagctc caacagccgg tagtgcccgt ttcgacacag
     2761 tgtggatagc caccgtcgga aatcgggctt gacaactgta attgcggaga gtacagtttc
     2821 gctatgaaaa gcagtgggaa cgtcacgagg caaccgcaga tcggccagag ttggttcgct
     2881 atggccgtcc atatccttgc cttgcttgca cgttcgcccg agggctaccc tagcgcctac
     2941 ctcgcaggca gcgtcaacac ccacgcggtt ttcctgcgca aggtcgtggc gcatctggtt
     3001 cgggcgggcc tcgttgtggc acaggagggg cgagagggcg gctatcgtct ggctcggccg
     3061 gccgatcaga ttagcctggc ggacgtctac cgtgtgatgt acgcgggtgg cccgcttccg
     3121 cccaaccccg ccgagccgaa ccccctctgc gaggtcggct ctgggatgag agcggccctg
     3181 ggcgaagtgg catccgaggt ggagcagcag atggtgcgcg acctggagag acacactatt
     3241 gcagagatct cggatcgtgc cgtgttgctc gggaactttc cgaacgggag gtaacggagg
     3301 atatccagaa gcgaggctgg tccggcctct cttttttgcc actaactgta atgatgcgca
     3361 ctacagttat gggggtgcca agcgccgttt ggccttgaac cgcgtccttg tggcgccaca
     3421 agcacctcct atcgacgttt ggtactgtca agaccgacaa gtcgtaaaga ctacaggaag
     3481 tgaaaggaag agagaagtga aaatcgctat caccggagct tcaggtcaac tcggccatct
     3541 agtaatcaac aagctcaagg ccaagattcc tgccgccaat atcgtcgcgc tcgcacgcac
     3601 agcttcgaag gccgccgacc ttggtgtcac ggtccgcgag gccgattaca ctaggcccca
     3661 gacactcgac catgccctcg ttggcgtgga cacgctactg ttcatctccg gaagtgaggt
     3721 gggccaacgt gtagcccagc atcacaacgt catcgaggcc gcgaagaggg caggagtgaa
     3781 gtgggtcgtc tacacgagcc tcctccgtgc agacacctct ccattgagcc ttgccgacga
     3841 gcatcgcgcg acggaagctg agctgaaggc gtcggacata ccgttcacgc tcctgcgcaa
     3901 cggctggtac accgaaaact acaccggctc ggtccccggt gcaatcgccg gtggcgcatt
     3961 cctcggctgt gccgacgatg gcaagatctc atccgcgacg cgtgacgact acgcggaagc
     4021 ggcggtcgct gtgctgacca gtgaaggaca tgtcggcaag acctacgagc ttgccggcga
     4081 cgacgcctat acgctcagtg atctggccgc tgagatctcc cggcagaccg gcaagacgat
     4141 tccttacaag gatttgcctg aagccgagta cgctgcggca ctcaccggcg ttggcttgcc
     4201 tgaaggtcta gcacgggcat ttgctggata tgacgtcgcg gcggcaaggg gtgcgctgtt
     4261 cgacgacggg cgccagctct ctgcgctgat cggtcgtccc tcgacaccgc tgtcggtcgc
     4321 cgtggccgac gtgctcaagt agcacatcga cggacggcat ctgcccgaaa tggacaatcg
     4381 agcgcgacgt ttgcccattc gcaatcgttc cgacacgatc ggagtagaga ggacagtctc
     4441 ccagtgagcc gacaacacga acacgaaggt cctgaggctg atcgcggcta caggctcctg
     4501 agacttgacg actaccggga actggtctca ctggacgctc tcttcaaggc cggctcccct
     4561 atcagcccgg agagccggaa cagggtagta gacgacctcc tggagacgct cgcgccggag
     4621 ggcgcttcca ggaggttggg gctgcggccg gaggccatcg agagactgaa gagagatcca
     4681 gagcaggctc gtcgtctgct gcgggcgttc ctgacggtac gtggccccga accactgctc
     4741 gaatccttca acttgatgct cgatggactg ctccagctgg agctacagga gcgaggtcag
     4801 gttgagctgc ctagcctaac ccgaatacgc gaccttctgc cggagagcag ctatccggcc
     4861 ctcccttact gtgcgctctg gcggggagat atcacgcggc ttagcgtcga tgcgatcgtc
     4921 aacgctgcca actccaggat gctggggtgc ttcgttccgt tccacgcctg cattgacaac
     4981 gccatccatt cgacggcggg gccgcggctg cgcgaggact gcgaccgtat catgcgcctg
     5041 cagggcggtc cggagccgac gggaggggcg aaggccacac gtggttacaa ccttccggcc
     5101 cgtttcgtcc ttcacacggt gggcccaatc gtcgagaaag agctcacgga ggcccacgag
     5161 aaagcactcg cctcctgcta catctcatgt ctcgatctag cgtcgcgtct gccagacgtg
     5221 cactcagttg cgttctgtgc gatctcgaca ggggtcttcg gctatccgaa gctgccagcg
     5281 gcgctcgtcg ccctccagac cgtctccgag tggcttcggg cgcatcccgg atcgcttgac
     5341 ctcgtggtgt tcaacgtctt cggggacgag gacctcgccg tctatcaagt agctctggag
     5401 aagtgggact ccgcgtcttg cgtcaggaga actaaacagt gattcatcac gagagagccc
     5461 actatcattt ggacgtacca aggattgtga aatggctcga ggaaagcacg cacgtcctga
     5521 taggggctgg atctggcatg actgcctcgg tgggcatcga ctacacagac cctgtgagct
     5581 ttgccagact ctttcctgcc ctcgtccggc gcggcttcaa ggcccggtac gagctgatcg
     5641 ggtacgagga ttgggctccc gaggagaagt gggcgtactg ggcgacccac gtgaacgacg
     5701 tgcgcttcgg gtcgcgccac gatcaggtct accgccaact cctcgacctc gtcgtaggcc
     5761 gggactactt tgtggtgacc tcgaacgtag atgcactctt tgcccgtaac ggctttgagg
     5821 agaagcgact cttcaccccg cagggcgact acgcgcttct ccagtgccgt aaaccgtgca
     5881 cccgccgagt ttggccgttc aggcccatca tggatcagat cctgccggcc atcgatcctt
     5941 acagcttcag ggttcgcgac tctgcggttc tccctcgctg ccccgactgc ggcggcgagg
     6001 tcttcctgaa tgtccgagtc gatggctcgt tcatcgatga cccctacgtc gagcagtatc
     6061 accggctctc ccattggctg cgagaagcga gagaaggggc tctcctggtt gtcgagatcg
     6121 gtgccggctt caacacgccg agcgttgtcc gctggccgat tgagggcgtt gtcgagacta
     6181 ctcccggcgc gaagctggtt cggataaacc tctcgcagcc tgaggtgcca tcggagatag
     6241 cggatcgctc gatttctgtg aggtctgacg ccggcgaggc aatcgacgcc atcacccgag
     6301 ggctacagcg aaggtagctg attgggcgaa cggcgcgcgc aggccgatcg gcctctcagt
     6361 tagtccagtc cggggaccaa caacaaccct cttgacaccg tatcgcgact ctcgtagaat
     6421 gtgctagaac aaatgttcta gaaaggaggg tgagcaggat gcggtgtgag gccgtggtca
     6481 agaggacggg ggagcagtgc ggccgggaag cctctcgcgg cagccgattt tgccggtatc
     6541 atcgccgcta cgcgtccggc ggcacgttgc cgggtgatcg cccttccttc tattccgacg
     6601 tgatggaaga gggggcgctg gagttggagg tggcttccgc actgcatggt gtggaggacg
     6661 agatcgccgt gctgcggatg ctgatccgca gggttgcccg ggagggtgat ctcgaggcca
     6721 cgcgcaaagg gatagagacc ctctgtcgtg ccctcaaggt gcagtatgcc ctggatgggc
     6781 gttccgccga tggtctcgcc ggctcccttg ccaaggtgct ggacgaggtg ggcaacgagc
     6841 tgggaatgag tctgtgagga gactgagatg gcagaggacg gttctgaggc tttgaggtcg
     6901 gcgctcaagc ggttttcccc gccgtccgct ccccatatag gaggtccgga tgacaggggg
     6961 atcagggagt tggcgtgccg gctggacgat cggggatgcg catacggccg gatcagcaac
     7021 gccaggctgg aggatctgtc caggggctac gagcgggttg aggctaagct gaatgccgtg
     7081 ctgttggctg ccgtcggcac cttcgttagc agcctggtgg gcatggccgt ctatcacctg
     7141 cgaggtggat gaggttggag cgctgcgtgg agttggaggc gagccccgag tatcaggagc
     7201 gacaggagcg ggaggagcgt attacgaggc tggccttgcg ctaccgttca ggggatcgtg
     7261 gagtgcttgg ggaactgtac gctgcggtgg agccgctgat ccaccacttt ctccggattc
     7321 acctctcccc tccccgctca ctcccctgcg gggtcgagcc ggcggacctc taccagcagg
     7381 ccttcgtggc gctggcggat accacgctgg aatgggatcc ggagcggcgc gacaacttca
     7441 tgccctactt ctttcactcc tttccctggc ggatagatca ctacctgagg gcccagagtc
     7501 ccgcgcgtcg aagctcgcgc tttcaactct tcagcctgcc gcacgacgta ctggtggagc
     7561 ggatggatag catgcctgga ccggatgggc gggattggga tggtgcgctg gcctgtgcgg
     7621 agttgatggg cgagcttcct cggcaatatg cgcaggtggt caggctgcac cttttccacg
     7681 gtctcccttt tgctgaggtc ggtgcggcgc tggggatggg ccgatccgcg gcgcacgaga
     7741 gcttcggccg agccatggcg ctgctgaggt cgcggctgga tggggcccca agggtgagcg
     7801 gggtttccgg aggggaagga gcatctcatc ctccggaggt ggactcggcg gtgctgcgcc
     7861 gctgtgtaga ggtgatgcat cggctggcgc cgggcaactc tccccttccg gggaggacgg
     7921 ctatctgcag cgcggccggc ttgaccggcc gggagtactt gagaataatg gagcggttgt
     7981 gcgcccgggg gtgcgtggtg gggcgacggc cggggagtcc cggtagtctg gcctatgccg
     8041 gcccggatga gacgctgcga cgactgaacg ggttctgagt tctgggacga aagcactcag
     8101 aacccagaac ccagaaccca gaactggaga ggcatggatc tcacgctgac cttcctcgga
     8161 acgggcacct cccacggcgt gccggccatc ggctgccggt gtcccgtctg tacctcggac
     8221 gatcccagga acaaccgcta ccgctgctcg atcctgctgc agtatggcgg ccacaacgtg
     8281 gtggtcgata cggctactga gttcaggctt caggcgatcc gcatcggact ggacagactg
     8341 gatgccgtgc tcttcaccca ctcgcacgcg gatcatatcg gcggcctgga cgacctccgc
     8401 tccttctccg agcgtcaggg cgcggcgatc ccctgttacg gcagtgcgga gtgcctggac
     8461 gatatccgcc accggttcga ctacatattc aaggaaaccc agttgggcgg aggcaagccg
     8521 cgcctggata tgcacccggt ggatggcccc ttcgaactgt tcggcctgag gatcgttccg
     8581 gtgccggttt accacggccg gttgctggtg ctcgggttcc gctttggccg gacggcctac
     8641 gtcaccgact gcaactctat ccccgagggc tcagaggaga tgctgaaggg gctggacgtg
     8701 ttggtgctgg acgctctgcg ctggcgttcc catcctacac acttcaacgt cgaggaggcg
     8761 ctggcggagg tggcgaagct gcgacctcgc cacacctatt tcactcacct cacccatgac
     8821 ctggaccacg ccactaccaa cgctcagctg ccgcagggag tcgagctggc ctacgacggg
     8881 ctgaccctcg cactggaggt gtgagagcga cgcttgtttg aaggcttggc tgctaccggc
     8941 caggctatcg agatcaccta tgtcctctca taggggccat aacgcgagcg agcgcagcgt
     9001 cacgctcgtg ccccgcccaa agcgtcgtct ttaatcacct tggtggtcgt ggccacggtg
     9061 tgggaggcgg ggcacaagac cccgccctac gattatcctg gatgtgctcc actctttgac
     9121 caaaccgcgt cacacggaat gcgtgcatct ccaggacttg gcgcgctggt acactacctt
     9181 ccagccctag cccctagccc ctagccccca gcccggtatg gttgttgcct gacgtgcccg
     9241 cgtatgaacg gcagatgggt tgtcaaagcg gcgttgctgt ttctcctgct gctggcgccg
     9301 ggcgccatat cggccggaac cggcgccctt tcgggcgggt tacccggcat cccggatcgc
     9361 gcctcgactg tgccctcgca gccccgacct accgctacgg ctgaagcgat cgctccgacc
     9421 cccgagccaa ctcctgagcc aacgcccatt ccagatccct ccctctaccg gctgcctgcg
     9481 aactggcaga agggggtcgt cctgtgggat aggcccgagt ccttcggctc gccggcgagc
     9541 tttcagtcgc tgcgcgaact gagagacgtc ggcgctacct ccatctcgcc cgtagtcacc
     9601 tggtacgtcg ccggcccatg ggatccccac attcatcccg ggaagaagac cccgaccgac
     9661 gacgatctga ttgcggtcat cgacgaggcg cacaacctgg ggctgcaggt aatgctgcgg
     9721 ttccacatcg actgtgagga cggcaactgg cgggcgcgca tagatcccca ggacaaggat
     9781 gcgttcttca atgcttacac cacggtgctc ggatactacg cagggttagc ccgagaccac
     9841 gcggtggagg ggatcgcgat cggggccgag ctctcctcgc tcacaaagcc cgcgtacacg
     9901 gactactggc gatcggtcat agtgcaggtg aggcagcgct tcccgggttt tctcacctac
     9961 agcgcccagt ggggctcggc gcctccagat gccctggccg atcctttcag ggagttcgag
    10021 cagatccagt tctgggacga tctggactac ctgggcatct ccggttactt cgagttggtg
    10081 gccgccggac agacgccctc actccagagc tacatagaca tgtggagtcg gtggcgtcgc
    10141 cttaggatcg agccgttcca ggccaagtac aacaaaccgt tgatcttcac ggagatcggt
    10201 tacgccagca ctcccaccgc ggcgatccat ccgtgggccg ggggtggggc cggggcgata
    10261 gatctccagc ttcaggctga gctgtactct gcccttttcg agagttgggc ggacgttccg
    10321 tggttcaggg gggcctactt ctggttctgg ccgacatcac cgggtgggct tgggctggag
    10381 ggtgccgatt accctccttc gggcaaacca gcgcaggagg tgcttcgtcg ctggtatcgt
    10441 ctactggacg acatggagca gccaccctgg aaaccgtcta gcgcaggggg tacaattcca
    10501 caggaagaag ggaggtgagt agtatggcga cgaagagcac ccccgtgccc ggcatcatcg
    10561 ggccgggacc gcagggaggg ggcccgaaca tccaggtggt ccgccacggc aggctgacct
    10621 gggtgaacct ggagcgcccc accaaggatg agatcgagtg gctccgccag aactactcct
    10681 tccaccccct ccacctggac gactgcctga gcaagatcca gcggccgaag atcgacgagg
    10741 agaaggacta cctattcgtc gtactccact ttccggtgtt caaccgggtc gcgggcgtca
    10801 cggttgccag cgaggtggac gccttcatcg gtgcggacta cttcatcacc gtccactccg
    10861 gcgatatcaa gccgctgcgc aggatgttcc aggagtgcca caacaatccc gcctaccgcc
    10921 aggagttgat gtgcaggagc agcggctacc tgctgtacaa ggtcatcgac cggctggtgg
    10981 actactgctt cccgatcctg aacaagatcg acgccaacat cgaagcggtc gaggacacga
    11041 tcttcgagga gaacgtgcgt cagacggtga gggacatttc ggtgatccgc cgggacatca
    11101 tcaattttcg gcgcatcgtg aagccgcaga ttgcagtgat cgccagcctg gagcacaagg
    11161 agcgacccta ccttcaggag gacctggagg catacttcgg cgacattgcc gaccacgtgg
    11221 cgaagatctg ggatatcctg gaggactaca aggaggtgat cgaggggcta tccgacacca
    11281 acgactccct cacctcctac cggatcaacg aggtgatgaa gatcctgacc accatctcgg
    11341 tgatcatgct cccgctgacg ctggtgtcgg gaatatacgg gatgaacatc gggctcccgc
    11401 tccaggagca ctccatggcc ttcctggtgg tgatggggat catgctggcc atcgcgacgg
    11461 ggatgcttag ttatttccgc aagcgtggct ggatctgagg tagatatcag gaattctttg
    11521 atgattctgg ggacacccca gaccccgacg gaaggggccg aaggccccct ctgcactccc
    11581 ccaactggct ggctatagct tctgtagggc gcggcggtgg gcgtcgaagg cgatctccag
    11641 gggtagttga tcgagcggga agctcttcag ctcggcggcg tcgtcccccg ccttcggctc
    11701 tccatccacg atcctccccg tatacaccac cagaaggcag agccccctcg ggtcgccccg
    11761 ctccatctcc acgctcacca ggccggtcag ttccacctca accccaagct cttcccacat
    11821 ctctctcacc gcggtctctt ccggggattc gccatactcc atgaaaccgg atgggagagt
    11881 ccactgccct ttgaagggct cgaacttgcg gcgggccatc agcactcg
//
LOCUS       DATALS010000374         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_175048, whole genome shotgun sequence.
ACCESSION   DATALS010000374 DATALS010000000
VERSION     DATALS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175048"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1106)
                     /gene="ccsA"
                     /locus_tag="VHS06_08400"
     CDS             complement(54..1106)
                     /gene="ccsA"
                     /locus_tag="VHS06_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX2988174.1"
                     /translation="MAEIGRISLVVAFVVTAYLTVASAIGITRRQADLLASARNGVYT
                     ATALVTLAWGTLLYAFATHDFTMKTVANYNSRDTDLAYSLSGAYAGQEGSLLMWAWGV
                     TLMLLIVTLQNRGRHKSLMPWVTAVMGFVATFFLSLLPFVSDPFARMATVPADGQGLN
                     PLLQNPGMLFHPPALYLGYVAFTVPFAFALAALITGQLSNEWIQSTRRWTLFAWTMLG
                     IGNILGAQWAYVELGWGGYWGWDPVENSSFMPWLTGTAFLHSAMIQRRRGMLKVWSLA
                     VIIVTFILTIFGTFLTRSDVLSSVHTFGETNVGPMFVGMMVLLLAVSAALIWVRLPLL
                     EGENEERRWPQSPSRQ"
     gene            complement(1195..1668)
                     /locus_tag="VHS06_08405"
     CDS             complement(1195..1668)
                     /locus_tag="VHS06_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX2988175.1"
                     /translation="MLTEDTAGNSTRAPHARSTGRGTTRHRPRTRLKFLVAGLIIVVA
                     IGYMIYAAIQSGSEYFLTTGELRAMGEKAVGQQTKLGGRVVEGSLQGDKGGNTITFVL
                     TDGNQNIPVSYRGTVPDSFQPGTDCIVEGKLTAGGTFQATGLMAKCASKYTPANR"
     gene            2113..>2679
                     /locus_tag="VHS06_08410"
     CDS             2113..>2679
                     /locus_tag="VHS06_08410"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822926.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX2988176.1"
                     /translation="MIVVMNSHALENQIQGVLDRLRSKGLDGHRSGGEERTVIGVIGT
                     RFEPQLGEQLEMLSGVERVTPISKPYKLASREFQPFDSVVKVGDLEIGSGELAVMAGP
                     CSIESMDQMMETALAVKAAGARILRGGAFKPRTSPYAFQGMGEEGLKILSEVGQKTGL
                     KVITEVMEPEQVDVVAANADILQIGARSM"
BASE COUNT          538 a          759 c          854 g          528 t
ORIGIN      
        1 gctgatcgtg acgctgcaga atcgcggcag gcacaagagc ctcatgccct gggtcactgc
       61 cgtgatgggc tttgtggcca ccttctttct tcgttctcac cctccagcag cggcagacgg
      121 acccatatga gcgctgcgga cacggcgagg agcagcacca tcattcccac gaacatagga
      181 ccgacgttgg tttccccgaa ggtgtgaacc gacgacagga cgtcgctcct ggtgaggaag
      241 gtgccgaata tggtgaggat gaaggtcaca atgatcaccg ccaggctcca gaccttcagc
      301 attccgcgac gtcgttggat catcgccgag tggaggaagg ccgtgccggt aagccagggc
      361 atgaaggaag agttctccac tgggtcccag ccccagtaac cgccccatcc aagctctaca
      421 taagcccact gggcacccag gatgttgccg atgcccagca tggtccaggc gaagagggtc
      481 cagcgtcgtg tgctctggat ccactcattg ctcagttgtc ccgtgatgag ggctgcaagt
      541 gcgaaggcga agggtaccgt gaaggccacg tagcccaggt agagggcagg tggatggaac
      601 aacatgcccg ggttctggag caacggattc agcccctggc cgtcggcggg gacggtggcc
      661 attcgggcaa acggatcaga gacgaagggg agtaacgaca gaaagaaggt ggccacaaag
      721 cccatcacgg cagtgaccca gggcatgagg ctcttgtgcc tgccgcgatt ctgcagcgtc
      781 acgatcagca acatgagggt gacgccccat gcccacatca gcaacgatcc ctcctggccg
      841 gcgtaggcgc cggagaggct gtaggctaaa tcggtgtcgc gagagttgta gttggccacg
      901 gtcttcatgg tgaagtcgtg ggttgcgaat gcatagagca gtgtgcccca tgccagagta
      961 accagcgccg tcgcggtgta gacgccgtta cgtgcgctgg ccaacaggtc ggcctggcgg
     1021 cgggtgatgc cgatggccga ggccaccgtc aggtaggcgg tgaccacaaa ggccacaacc
     1081 agggatattc gaccgatttc tgccatggtc cttcgctccg aatggggggg ggtgggggat
     1141 aggggatgtg ggatgggggc tagagaccaa cccctgaccc ccgatccctg tcccctacct
     1201 gttggccggc gtgtacttgg aggcgcattt ggccatcagg ccggtggcct ggaatgtccc
     1261 gccggccgtc agcttgccct ccactataca atccgtgccg ggctggaagc tatccggcac
     1321 cgtccccctg tagcttactg gtatgttctg gttgccgtcg gtgaggacga atgtgatggt
     1381 gttacccccc ttatcgcctt gcagggatcc ttccaccacc cgaccgccaa gcttcgtctg
     1441 ctgccccacg gccttttcgc ccatcgctct cagttcgccc gtggtgagga agtactccga
     1501 gccgctctga atggccgcgt agatcatgta tccgatggcg acgactatga tcagtcccgc
     1561 gaccaggaac ttcagtctcg ttctggggcg atggcgtgtc gttcccctgc cggtcgagcg
     1621 ggcgtgggga gcccgcgtgg agttcccggc ggtgtcctcg gtcagcatcg actctctcct
     1681 aacgtcacga agcgtccctg cagctaccgc gtgaagcttc tctctgtcct cgcctcggat
     1741 tactatcggt tgccaaagag aaattatagc atcgtggctg ctagcctctt accgcgatgg
     1801 gccaggctac ttgcggcggt ttctcgcgat cgccacaggt ttgtgatttt gcggttgaca
     1861 gcttccaata acaagcctat actcccattt aactggcccc gggtcaaccc ggggagtagt
     1921 ctattcagga ctcgatgata ccgattagtt attctgagtt ttacttttac ggtacggcat
     1981 gacggggcgc ctcctcagca atagctgttg aggaaccctg gaaccccgtc atgccgaagg
     2041 agcagaggag aaatcctctg ctccttcgtt tttgtccgga attcgccgct cgtgaaaggg
     2101 aggagcagac cgatgatcgt cgtgatgaac agccacgctt tagaaaacca gatccaggga
     2161 gtgctggatc gtttgcgcag taaaggcctg gatggccacc gttcgggagg ggaggaacgc
     2221 accgtgattg gggtaattgg aacccgtttt gagccacaat tgggggagca gttagaaatg
     2281 ctctccgggg tggagcgagt cacacccatc agcaaaccgt acaagctggc cagccgagag
     2341 ttccagccct tcgactcggt ggtcaaggta ggggatctgg agatcggtag cggagaactg
     2401 gcggtgatgg ccggcccctg ctctatcgag agcatggacc agatgatgga gacggcgctc
     2461 gcggtcaaag cggccggggc caggatcctg cgcggcgggg cattcaagcc tcgcacttca
     2521 ccatacgctt tccaggggat gggagaagag gggttgaaga tcctctcgga ggtcggccag
     2581 aagactgggc tgaaggtcat caccgaggtg atggagcccg agcaggtaga tgtggtggcc
     2641 gcaaacgccg acatcctgca gataggggcc cgcagcatg
//
LOCUS       DATALS010000375         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_424417, whole genome shotgun sequence.
ACCESSION   DATALS010000375 DATALS010000000
VERSION     DATALS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424417"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..213
                     /locus_tag="VHS06_08415"
                     /pseudo
     CDS             1..213
                     /locus_tag="VHS06_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
     gene            205..657
                     /gene="ribE"
                     /locus_tag="VHS06_08420"
     CDS             205..657
                     /gene="ribE"
                     /locus_tag="VHS06_08420"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX2988177.1"
                     /translation="MRFRGLRFGVVISRFNEFISGRLLAGAKDALIRHGVDEEAVDVA
                     WVPGAFEIPLVAKKMAQTGSYSAVICLGAVIRGATPHFDYVAAEVSKGIANVSLDTGV
                     PVLFGVITADNLEQAIERAGTKSGNKGFDAATAAIEMANLVSALRSER"
     gene            complement(660..1274)
                     /gene="plsY"
                     /locus_tag="VHS06_08425"
     CDS             complement(660..1274)
                     /gene="plsY"
                     /locus_tag="VHS06_08425"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX2988178.1"
                     /translation="MNLLSFVAVAVAAYLLGSFPSGVVLARLRGNVDILNKGSKRTGA
                     TNVLRTMGWKAAAPVFAMDFVKGILAVAAARIISGGDPYADVIAGLAALLGHNYSLFI
                     RFRGGRGVTTGLGAVAVISPVALLVAAVIGVVIVARTRYVSLGSVVGAGMVPLALLPL
                     VLAAGQPFPHLMFGLLGSAFIIASHRDNIGRLLHGTERKLGERA"
     gene            1339..2292
                     /locus_tag="VHS06_08430"
     CDS             1339..2292
                     /locus_tag="VHS06_08430"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX2988179.1"
                     /translation="MKVDEKICVLNAGGWGTALAVLLSGKGYDVRLWARRPELAREIG
                     VTRENLSYLPGVSIPRNVLATSDLREAVDGSSVVVVATIFRGLKELAPRIGALLRPDQ
                     LVVCAAKGLDEETLRRGSELLENSLSTDHRGRVAVLSGPNHAEEVSRGIPTATVVASR
                     DEEVALRIQEVLSAPTFRVYTNLDIVGVELCGALKNVVAIAAGMSDGLGYGDNSKAAL
                     ITRSLAEIGRLVVEEGAHPYTVAGLSGIGDIIATCTSNHSRNRAAGEAIARGKSVEQV
                     IAGSPMVVEGIPTTRAILKLARQKGVEMPITRQVHAVLFHN"
BASE COUNT          459 a          695 c          795 g          406 t
ORIGIN      
        1 atcctcaaca aggggagcaa gcgcaccggc gccaccaacg tccttcgcac catgggatgg
       61 aaggccgcgg cgccagtttt cgcgatggac ttcatcaaag gcatgctcgc ggtcgcggcc
      121 gcccgcctca tctccggcgg agatccctat gccgacgtca tcgccggact tgccgccctc
      181 ctgggacaca actactcgct ctttatcagg ttccgggggc ttcgcttcgg cgtggtgatc
      241 tcccggttca acgagttcat cagcggaagg ctgctggccg gggcgaaaga cgcgctgatc
      301 cgccatggcg tggacgagga agctgtggac gtggcgtggg tcccgggagc cttcgaaatc
      361 ccgctcgtag cgaagaagat ggcccagacg ggcagctact cagcagtgat ctgcctcgga
      421 gcagtgatcc gcggagccac ccctcacttc gattacgtgg cagccgaggt ctccaagggt
      481 attgccaacg tgagcctcga caccggtgtg ccggtgctct ttggggtgat cacggcggac
      541 aacctggagc aggcgatcga gcgcgcggga accaagtccg gaaacaaggg tttcgacgcg
      601 gctacggcgg ccatcgagat ggcgaacctt gtctccgcgc tgcgctcgga gcggtagcct
      661 tacgcccgct ctcccagctt gcgctcggtg ccgtgtagca gccggccgat gttgtccctg
      721 tggctggcga tgatgaaggc gctacccagg aggccgaaca tcaggtgggg gaagggttgc
      781 ccagcggcta acaccagcgg cagaagggcg agaggcacca tccccgctcc cacgaccgag
      841 cccagggaga cgtaccgcgt tcgagccacg atcaccactc caatgacggc tgcaaccagc
      901 agcgcaacgg gggagatgac ggccactgca ccgagtccgg tggttacgcc ccgaccccct
      961 cggaacctga tgaatagcga gtagttgtgt cccaggaggg cggcaagccc ggcgatgacg
     1021 tcggcatagg gatctccgcc ggagataatg cgggcggccg cgaccgcgag gatgcctttg
     1081 acgaagtcca tcgcgaaaac tggcgccgcc gccttccagc ccatcgtgcg gaggacgttg
     1141 gttgcgccgg tcctcttgct tcccttgttg aggatgtcga cgttcccccg taggcgggcc
     1201 aggacaaccc cggaggggaa ggagccgaga aggtaggcgg cgaccgccac ggcgacgaac
     1261 gacagtaggt tcatgtatac ctcgatcggg ctataattcg aacagactcc aggatagcat
     1321 accgcgggag acgtgggatt gaaagtagat gagaagatct gcgtgttgaa cgccggcgga
     1381 tgggggactg ctctggccgt gctcctgtct ggcaaaggtt atgacgttcg tctatgggct
     1441 cgaaggccgg agctggcaag agagatcggc gtgacaaggg agaatctcag ctatcttccg
     1501 ggcgttagca ttccgcggaa cgttcttgcg acgtccgatc tccgtgaagc agtggatgga
     1561 agctccgtcg tggtcgtggc caccatcttc cgaggcctga aagagctggc accgcggatt
     1621 ggtgcgctac tgaggccgga ccaactggtg gtgtgcgcgg cgaagggact cgacgaggag
     1681 acccttcgca gaggatcgga actgctggag aattccctgt cgaccgacca tcgtggaagg
     1741 gtggcggttc tgtcgggacc caaccatgcc gaggaggtga gtcgtgggat ccctacggcg
     1801 accgtcgtcg ccagcagaga cgaggaggtg gctcttcgga tccaggaagt cctctccgcg
     1861 cctaccttca gggtgtacac caacctggat atcgtcgggg tggagctgtg cggtgcgctg
     1921 aagaacgtcg tggccatcgc ggcaggcatg tccgacggac tcggctacgg ggacaattcc
     1981 aaggctgcgc tgatcacccg cagcctcgcg gaaatcggca gactagttgt ggaagagggc
     2041 gcgcatccgt atactgttgc aggtctttcc ggcatcggcg acatcattgc gacctgtacc
     2101 agtaaccaca gccgcaacag ggccgctggt gaggcgatag cccgcggcaa gagcgtagaa
     2161 caggtgattg ccgggagccc catggtggtc gaagggatcc ccacgacccg ggcaatcctg
     2221 aagctggcgc ggcagaaggg cgtggagatg ccgataacga ggcaggtcca cgcggtcctc
     2281 ttccacaact agtctgccga tttccgcgag gctgcgggtg atcagcgcag ccttggaatt
     2341 gtccccgtag ccgag
//
LOCUS       DATALS010000376         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_175394, whole genome shotgun sequence.
ACCESSION   DATALS010000376 DATALS010000000
VERSION     DATALS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175394"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VHS06_08435"
     CDS             <1..856
                     /locus_tag="VHS06_08435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2988180.1"
                     /translation="FNRINWGRLTFTNTITTVSRYMKHIMWGVGCDPLVIPNGIPRRA
                     LDPVDEGAVARVRGALGGDPLLFKIGRFDPDKRWNMAVEAVARLKGMGMRPVMPIRGG
                     IEPHGNEVIGNARRLGLSVREVRSEGRSVEECVQALESAGPADILDLRFFLPDEFVRI
                     MYRAADAVLANSGHEPFGLVGLEVMGAGGIAFTGSTGEDYAIPFENSMVLDTSDPGEI
                     AGYVVHLQNHPEEKERIRAGAARTAATFVWDRVLENLVSKLEYIARNQGVLAAEDVGP
                     AVVGGRRG"
     gene            849..2054
                     /locus_tag="VHS06_08440"
     CDS             849..2054
                     /locus_tag="VHS06_08440"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEX2988181.1"
                     /translation="MANDLVVYTIIHQPRRIKLPAQPIPRGAVPADIERCLFDERMNE
                     RYFRKVGKWCYYPATRTFLELVEEGFKLSIGFSVSFLKQAEMWDQELLGLFQKLVRHE
                     NVELIGVEPYHSFIMLVDLERFIQRMHWAGDYLYQLFGKRPVVTDTTEMCMSDSIYHA
                     LDKAGFKGAVLDGRPWVMGWRDPTYLYNAGKSLKLLTRDYELSDDVGYRFSNRTWSGW
                     PLSSDTYAHWLREARGDFVFLAWDYETFGEHHSKDTGIFEFLRWMPSEVRRRGMGFLT
                     PSEAIEKYGDRSYELPLSAFPSTWAGSGGMEFFLGNSAQQAVFQLMLHCYNTAVLTGN
                     TELEDLALWLLQSDNLHLIQWFGRGGSEAEVSAYFTPDEWWDLGPGGIIWEQQQVYRN
                     FLGSLDPYL"
BASE COUNT          391 a          586 c          751 g          421 t
ORIGIN      
        1 cttcaaccgg atcaactggg gcaggctcac gtttaccaac actatcacca ccgtctccag
       61 gtatatgaag cacatcatgt ggggggtggg atgtgaccct ctggtgatcc cgaacggtat
      121 ccctcggagg gcgcttgatc cggtggacga gggcgcggtg gctagggtaa ggggtgcgct
      181 gggaggggat ccgctgctct tcaagatcgg ccggttcgac ccggacaagc ggtggaacat
      241 ggcggtcgag gcggtggctc gcctgaaggg catggggatg cgtcccgtga tgccgataag
      301 gggcgggatc gagccgcacg gcaacgaggt gatcggaaac gcccgacggc tgggactcag
      361 cgtgcgtgag gttcgctcgg aggggcggag cgtcgaggag tgcgtccagg cgctggagag
      421 cgccggcccg gcggacatac tggacttgcg tttcttcttg ccagatgagt tcgtgcgaat
      481 catgtacagg gcggccgacg cggttctcgc gaacagcggg cacgagccgt tcgggctggt
      541 ggggctggaa gtgatgggag ctggcggaat cgcgttcact ggatccaccg gtgaggatta
      601 cgccattccg ttcgagaaca gcatggtgtt ggatacctcg gaccccggcg agatcgcggg
      661 ttacgtcgtc caccttcaga atcaccctga ggagaaggag aggatcaggg ctggggccgc
      721 ccgtacggca gccacctttg tgtgggaccg ggtcctcgag aacctcgtga gcaagttgga
      781 gtacatcgcc cgcaaccagg gcgtgctggc agctgaggat gtcgggccgg cggttgtggg
      841 aggccgccgt ggctaacgat ctcgtcgtct acaccatcat tcaccaaccg aggcgtatca
      901 agctccccgc gcagccgatc cccagggggg cggtcccggc ggatatcgag cgatgcctct
      961 tcgacgagcg aatgaatgag cgctacttca ggaaggttgg taaatggtgc tactatcccg
     1021 cgacgcgcac cttcctcgag cttgttgagg aggggttcaa gctatccatc gggttctcgg
     1081 tgtcgtttct caagcaggct gagatgtggg atcaagagct gttggggctc tttcagaagc
     1141 tggtccggca cgagaacgtt gagttgatcg gtgtggagcc ataccacagt ttcatcatgc
     1201 tggtcgacct ggagcgcttt attcagcgga tgcactgggc gggcgactac ctttatcagc
     1261 tgttcggaaa gagaccggtg gtcacggaca ccaccgagat gtgcatgtca gactcgatct
     1321 accatgcgct ggacaaggcc ggcttcaagg gggcggtcct ggacggccgg ccatgggtaa
     1381 tggggtggcg ggatcccacc tacctgtaca acgcggggaa gtcgttgaag ctgctgacga
     1441 gggactacga gctgagtgac gacgtggggt atcgcttctc caaccggacg tggtcggggt
     1501 ggcccctttc gtccgatacc tacgcccact ggctcaggga ggcgcgcggg gacttcgtat
     1561 tcctggcgtg ggactatgag acttttggcg agcaccactc caaggatacc gggatattcg
     1621 agttcttgag gtggatgcca tctgaggtga ggcggcgcgg catgggtttc ctcacgccga
     1681 gcgaggccat cgagaagtac ggtgaccgca gctacgagct gccgctttcg gccttcccga
     1741 gcacctgggc cggctcaggg ggaatggagt tcttcctggg caactctgcc cagcaggctg
     1801 tcttccaact gatgctgcac tgctacaaca ccgcggtgct gacggggaat acagaactgg
     1861 aggaccttgc gctctggctg ctgcagtccg acaacctgca cctgatccaa tggttcgggc
     1921 gtgggggatc ggaggcggag gtgtccgcct atttcacccc cgacgagtgg tgggacttgg
     1981 ggccgggcgg gatcatctgg gagcagcagc aggtgtaccg caatttcctg gggtcgctgg
     2041 acccgtacct gtaaggcgat atcacgtcgc gacttgccga ggttgcccgt aacgtctcgt
     2101 agggcggggt cttgtgcccc gcccagagcc gttccccaaa tcaccgtgg
//
LOCUS       DATALS010000377        11534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_224777, whole genome shotgun sequence.
ACCESSION   DATALS010000377 DATALS010000000
VERSION     DATALS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11534
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224777"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VHS06_08445"
     CDS             complement(<1..507)
                     /locus_tag="VHS06_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX2988182.1"
                     /translation="MLVDTHAHLTMLELAEELPSVLQRALDAAVSAIVCPGVDLPSSR
                     EAVDLARLHPRVRAAVGVHPNDCADLPPDWVDRLRSLAAEPEVVAIGEVGLDNYRQHT
                     RSDLQRAVLEAQLDLAADLDLPVVIHDREAHLELAEILHKWVARLPARHPRGVLHCFS
                     GDRAFMEHC"
     gene            complement(569..1408)
                     /gene="rsmI"
                     /locus_tag="VHS06_08450"
     CDS             complement(569..1408)
                     /gene="rsmI"
                     /locus_tag="VHS06_08450"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX2988183.1"
                     /translation="MGTLYVVGTPIGNLEDITLRALRVLKEVDLIAAEDTRKTMRLLS
                     HFDIHTPQESYHRHSGTPKLERLLELLQTKDVALVSEAGMPGISDPGEPLIAAALEQG
                     VPVIPIPGPTAMATALVVSGLPSDRFLFLGFLSRVRSDRQRFLRSVAALPFTLVLYEA
                     PHRLLATLADLETVLGDRPAAAARELTKLHEEVVRGRISEIALVFKERAPRGEFTLVI
                     APPREGEVSSEQAAQPAVDLVAAARRLRAEGVEGKDGVKRLMESTGSGRRAAYQAWLE
                     SGK"
     regulatory      1549..1653
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1751..2824
                     /locus_tag="VHS06_08455"
     CDS             1751..2824
                     /locus_tag="VHS06_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HEX2988184.1"
                     /translation="MPSELPAIGKISPEIFEEIIYPRLGAKSANVLVGPQHGVDVGVV
                     DLGHDQVMVLTTDPFFIVPQYGWERAAWFATHILASDASTSGLRPTYFTVDLNLPLSI
                     DREGLEIMWETVHRECERIGMSVVTGHTARYEGTDYPMVGGATVICVGPKSQYVTPNM
                     ARVGDQVIVTKGAAIEATGLFAATLPHLVAERYGEQFAKEADRVFEMMSVVDDALTAV
                     TVGVRDNGVTTMHDATECGVWGGLFEIAQASHVGMQIDKEKIIVRPEVSKVCELFKID
                     PYASISEGTLLMTCRPHKAAEAIKVLADKGIEASIVGECTPLEKGMVVSEGSTSRVLE
                     HPRVDPFWQAFGQAIATNKAPEA"
     gene            3132..3473
                     /locus_tag="VHS06_08460"
     CDS             3132..3473
                     /locus_tag="VHS06_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX2988185.1"
                     /translation="MLHCFSGDRAFMEHCCAMGFYISFAGPITYRNAGKIVETAAATP
                     RDRLLVETDSPYLAPHPHRGKRNEPAYVRLVVERLAEIRQEDLDGMIEVTGQNAARLF
                     RLELQPASHER"
     gene            3478..4452
                     /locus_tag="VHS06_08465"
     CDS             3478..4452
                     /locus_tag="VHS06_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX2988186.1"
                     /translation="MGSTPTASIFSLVHEELVAVEQALTRAVESEGPTMRRLLGIVLP
                     GSGKRLRPALALLCGRINSFRPYRLIPFAAALEMLHTATLVHDDIVDEADVRRGDPTL
                     NARFNNSVAVLVGDFMFAQAADHAVKAEDYRVITAFTRAAMTVIGGQIDESWANGSLV
                     MTQEQYLQRIGNKTAALFALAAEGGAMLSEASQPVIEAVKQYGYLMGLAFQIVDDILD
                     VVGDEGSLGKPVGSDVRQGTVTLPALMVRDRLTPEQFKAAFNGDGHREGPINLLLDTI
                     RREGGIELSYREADRLAADARAALKPLPAGEVRDSLEDLARFVVRRNV"
     gene            4465..5556
                     /locus_tag="VHS06_08470"
     CDS             4465..5556
                     /locus_tag="VHS06_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HEX2988187.1"
                     /translation="MKVTFPHAGQAWVPLSTVFGKAGVECIVPPPTSKRTLALGVKYS
                     PEWVCLPFKMNLGNFIEAMEMGADTLAYVSGSGLCRLGYYSKVSDQILRDAGYEFEMV
                     TFDWQETGVMGLANFFRRVLPDRPLRQIAGDIKFGLSQLKTMDDLERMTHRLRPRADN
                     PVEITRIWRTAGERIARAWSPADLKRARAELVDEFNSVPLNPYADPLRVGLLGEFFMV
                     VESFVNMDVEDRLGRMGVEVHRHAYFGDWARTWLFMEAIGLSHGKKVKKAAAPYLSRD
                     VSGDAVQTVGETILHQQEGFDGILHLLPFTCMPEIIAQNVMPTVTRDFSIPVLSIVLD
                     EQLGQAGFITRIEAFVDLMNRRRTVKRAS"
     gene            complement(5769..6053)
                     /locus_tag="VHS06_08475"
     CDS             complement(5769..6053)
                     /locus_tag="VHS06_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988188.1"
                     /translation="MSGVDSWDLNSIGDRRIGRQGRIKPYSEVQLFFLLRLERLLRVR
                     REHAALSASNDWWAKLLSRAIYSTYCDCIELGVGEDARSLLSRDQATAQS"
     gene            complement(6097..6330)
                     /locus_tag="VHS06_08480"
     CDS             complement(6097..6330)
                     /locus_tag="VHS06_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX2988189.1"
                     /translation="MQRAYVLIETLGGKSKEVADALRKVSGIAASDVVTGPYDVIAVA
                     QGADANVIGRLVLNEVRGIPGISKTMTCLAVDS"
     gene            complement(6604..7959)
                     /locus_tag="VHS06_08485"
     CDS             complement(6604..7959)
                     /locus_tag="VHS06_08485"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX2988190.1"
                     /translation="MGATRSLSEFAVRTTFDRLPTEVVRESKRCILNYLGVALGAADH
                     QAVRLLMETIAEEGGKRQATVLGHGRRCSAIQAATINGTMSHVLDYDDTHLPTVIHPT
                     GPVLSAALATAEWKGLSGRELIAALATGMEVELRVGSAVYPAHYDVGWHITGTAGTFG
                     AAAAVGKLLQLSEEQMTHALGMAGAQAAGLREMFGSMTKALHVGKAASNGLLSAMLAT
                     RGFTASTEVLEGRRGFCAVTASEYDLGQITVGLGDEYLMLQNGIKPYACGVVTHPTID
                     GVRRLRERYRLAPNDVLEIETKIHPLVRELTGKKSPRTGLEGKFSIYFCAAIALIEGN
                     ARESQFTDEKVNRPDVIALMDRVRAQVEPGVLEDQAVVSIRTVDGRILVERVEAATGT
                     PNNPMPDDQLMEKYLELSTRILPRDRAEQLAEQVAHMEKMEDPSGILEAATLRRRTGA
                     K"
     gene            8186..9139
                     /gene="xerA"
                     /locus_tag="VHS06_08490"
     CDS             8186..9139
                     /gene="xerA"
                     /locus_tag="VHS06_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HEX2988191.1"
                     /translation="MKQQVNAFLGYLAAERGLAANTLAAYNNDLSQFAEYLASRDQAG
                     IEGSGSEGSHQDGQGSLSVMGRDAVMGFFLHLREKGYTPSTIARKTAAIKSFFHFLAS
                     KGYVSEDPTASIDSPRISKSLPRAASVEEIDELLEAPTRISSPEALRDKAMFELLYAT
                     GMRVTELVSLNLGDVDVVQGCVQCSGRSAGKGARLRVIPVHATAIKAVQEYLEKARPM
                     LVRESDEMALFVNHRGDRLTRQGFWLILKQWAKEAGVTTQITPHTLRHSFALHMLKNG
                     VDLRAVQQLLGHAHISTTQIYTYPSEDGLKRLYETAHPRAK"
     gene            9140..9880
                     /locus_tag="VHS06_08495"
     CDS             9140..9880
                     /locus_tag="VHS06_08495"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2988192.1"
                     /translation="MVGAEERPTGEPTPTCRAVLFDAGNTLIYMPQTAEDILMALCHQ
                     LGAPIDLEAARGAYHRSESYYARNYLGYSGEQGAFWHAYHGEALRYLGIDDPDGRKAA
                     FLSHSFGRAGVWQAYPEAERVCSTLRAMGVRLGVVSNGPVTVSDLLTQAGLIPFFETV
                     IASQAMGIQKPDPRIFREALGRMGLRPNDALFGGDLYDVDVVGARSAGLRAVLIDRDG
                     HEGESDCPTIRTLDELIPLVTMFGARHV"
     gene            9873..10697
                     /locus_tag="VHS06_08500"
     CDS             9873..10697
                     /locus_tag="VHS06_08500"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX2988193.1"
                     /translation="MSDLSRAVELVSSIATIRPRVGIVLGSGLGMAAEGMEDAISIPY
                     RNIPGFPSTTVGGHRGELVLGTLEGLGVAALMGRPHLYEGYAPAQLGFSVRLLGALGV
                     ETLVVTNAAGGLAPNLEPGTLMLIEDHVNLPGMGGHNPLVGIEGGDERFVPMTGAYDE
                     TLRRVAQDAAAEAGIRLATGVYVMVSGPNYETAAEARFLRLLGADAVGMSTVPEVLVA
                     RWLGMRVLGISCITNLLLGPPSHAVDGHAGVLSAAAMAAPGMARLIRSVVRHLARQ"
     gene            10752..>11534
                     /locus_tag="VHS06_08505"
     CDS             10752..>11534
                     /locus_tag="VHS06_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988194.1"
                     /translation="MKIYSFLLPLMLVASTLIGFGYQGTTAAAPPAPAQETIPQTQLP
                     WRDLADLAVRLKGVSPDAVNGVPPQDVPEKQVGDQTSFWVADQANNRYYSVDATLKVV
                     TPHAYMYVADDSMLDIPRLQEAAQGFEDRVYTVDRHYFGSEPAVGLDGDAHVSILHAT
                     IPGLGGYFTSVDDYPRPVQGYSNERKSIYINVQVAPPGSLGYYAILAHEFEHMIHWNT
                     NREEQTWIKEGAAEEATEVTDLGGGSSVRDFEIAPDTQLNAWS"
BASE COUNT         2019 a         3702 c         3696 g         2117 t
ORIGIN      
        1 gcaatgctcc atgaaagccc gatcgcctga gaagcagtgc agcacaccgc gagggtgccg
       61 cgcgggaagc cttgcgaccc acttatgcaa gatctcagcc agctctagat gtgcctcacg
      121 gtcgtgtatc acaaccggaa ggtccaggtc cgccgccaga tcgagttggg cctccagcac
      181 cgccctctgc aaatcgctcc tcgtgtgctg ccggtagttg tccagcccca cctccccgat
      241 ggccactacc tcaggctcgg cggccaggga gcgcagccgg tccacccaat cgggcggcag
      301 atcggcacag tcgttggggt gtacccccac cgctgccctg acgcgcggat gcagccgtgc
      361 cagatcgacg gcctctcggc tggacggcag gtccacaccg gggcagacga tagcggaaac
      421 ggccgcatcc agagcccgct ggagcaccga cggcaactcc tcagcaagct ccaacatcgt
      481 taagtgagca tgagtatcta ccagcatcct tttccgattg tagcataacg tcgcagaggg
      541 gggccagact tggggagcac accaaaccct acttgccgga ctccagccac gcctggtagg
      601 cggctcggcg accggagccg gtcgactcca tcagccgctt cacgccgtcc ttgccctcaa
      661 cgccctccgc cctcaatcgc ctcgccgccg ccacaaggtc gacggccggc tgcgcggcct
      721 gctcggagga cacctctccc tcacgcgggg gtgcgatcac cagggtgaac tcccctcggg
      781 gagcgcgctc cttgaagacg agggctatct ccgagatgcg cccccggacg acctcctcgt
      841 ggagcttggt cagctcgcgg gcagcggcgg ccggtcgatc ccccagcacg gtctccaggt
      901 cggccagtgt agccagcagc cggtgggggg cctcgtacag aacgagggtg aagggaagcg
      961 cggcgacact cctgaggaag cgctgtcggt ctgacctaac ccgtgagagg aagccgagga
     1021 agagaaagcg atcggagggg aggccggaga ccaccagcgc ggtggccatg gcggttggac
     1081 cgggtatcgg aatgaccggc accccctgct ccaacgcggc cgctatcagg ggctcgccgg
     1141 ggtccgagat gccgggcatt ccggcctcgg ataccagcgc cacgtccttc gtctgcagca
     1201 gttccagcag ccgctccagc ttcggtgtgc cgctgtggcg gtggtagctc tcctgggggg
     1261 tgtgaatgtc gaagtgggac agcagccgca tggtcttgcg ggtgtcctcc gcagcgatga
     1321 ggtccacctc cttcagcacc cgaagggccc tgagagtgat gtcctcgagg ttgccgatgg
     1381 gggtgcccac aacgtacaga gtgcccatac cgcctcgtcc ccccgtgccg actgaatgcc
     1441 atctccgtag gggcaacgca tgcgtcgccc cacatacgga tgggccgggg ccaattatag
     1501 ccgattgtat gccgccaccg gttgttgtaa gatgagttca taaccgcata gggttgctag
     1561 gggggcgcgt cggcgctgag attgtggttt cggccactga ccctcgaacc tgatctgggt
     1621 aataccagcg aagggaagca ggacatcgaa cgcacggccg ccgtctcaca gagattgggc
     1681 ggcctttcgt ttctccccca cgcacccttc cgcgtatcta gttgtctaag taccggaagg
     1741 agagaaagac atgcccagcg aactgcccgc catcgggaag atctcgccgg agatcttcga
     1801 ggagatcatc tacccgcggc tgggggccaa gtccgccaac gtcctggtcg gtcctcagca
     1861 cggcgtggac gttggagttg tagacctggg tcacgaccag gtgatggtgc tcacgaccga
     1921 cccattcttc atcgtcccgc aatacggctg ggagcgagct gcctggttcg ccacccacat
     1981 cctggcctcg gacgcatcca cctcggggtt gcgccccacc tacttcaccg tggacctgaa
     2041 cctgccgctt tccatcgacc gtgaggggct agagatcatg tgggagacgg ttcaccgcga
     2101 gtgcgagagg atcggtatgt cggtggtcac cggccacacc gctcgctacg aggggaccga
     2161 ctatccgatg gtgggcggcg cgacggtgat ctgcgtcggc cccaagtcgc agtacgtcac
     2221 acccaacatg gctcgcgtgg gggaccaggt gatagtgacc aagggggcgg ccatcgaggc
     2281 aaccggactg ttcgctgcca cgctacccca cctggtggct gagcggtacg gcgaacagtt
     2341 tgccaaggag gcggacaggg tcttcgaaat gatgagcgtc gtggacgacg cactcacggc
     2401 ggtcacggtg ggagtccggg acaacggagt taccaccatg cacgacgcga cggagtgcgg
     2461 ggtctggggc gggctgttcg agatcgcgca ggcatcccac gtgggcatgc agatcgacaa
     2521 agagaagatc atcgtccggc cggaggtgtc gaaggtctgc gagctgttca agatcgaccc
     2581 ctacgcctcc atcagcgagg gcacgctgct gatgacctgc cggccccaca aggctgccga
     2641 ggcgatcaag gtattggctg acaaggggat cgaggccagc atcgtgggcg aatgcacccc
     2701 gctggagaag ggaatggtgg tgtccgaggg ctcgaccagc agggttctgg agcacccaag
     2761 ggtggatccc ttctggcagg cattcgggca agccatcgcg accaacaagg cgcccgaggc
     2821 gtaggccggg ggccaggggc caggggccag aggccagagg ccagaggccg ggggccgggg
     2881 gccgggggcc gggggccggg ggtatcgtaa cgtagggcgg gttgtctcaa cccgcccgtt
     2941 tcgtgcacac ccacgccagg cggttttaga caacccgccc cacaggacaa gtggagggtc
     3001 cgccggctat gccgggccga aagccagcag ggtgcccgcc atcaggggtg tgagaaccat
     3061 cgccgacttc accagccgga tcggcgcccg tttggtgggt cgcaaggctt cccgcgcggc
     3121 accctcgcgg tgtgctgcac tgcttctcag gcgatcgggc tttcatggag cattgctgcg
     3181 ccatgggctt ctacatctcc tttgccggcc cgatcaccta tcgcaacgcg gggaagatcg
     3241 ttgagacggc ggccgcgacg ccgcgggacc gcctcctggt ggagaccgac tcgccctacc
     3301 tggcgcctca tccccatcgc gggaagagga acgagcctgc ttacgttagg ctggtggtgg
     3361 agcggttggc cgagattcgg caggaggatc tcgacggaat gatcgaggtg actggccaga
     3421 acgcggcaag gttattccgg ctggagttgc agccagcctc acacgagagg tagcagcttg
     3481 ggctctacac ccacagcatc gatattttcc cttgtccacg aggagctggt tgccgtcgaa
     3541 caggctctga cccgggccgt cgagtccgag gggccaacca tgcgccggtt attggggata
     3601 gttcttcccg gcagcggcaa gcggcttcgc cctgccctcg ccctactctg tggacggatc
     3661 aacagcttca gaccgtatag gctgatcccc tttgccgccg ccctggagat gctccacacc
     3721 gccacgctgg tgcacgacga catcgtggac gaggcggacg tgagacgcgg cgacccgacg
     3781 ctcaatgctc ggttcaacaa ttccgtcgcg gtcctggttg gcgatttcat gtttgcccag
     3841 gccgcggatc acgccgtgaa ggccgaggat taccgggtca tcaccgcctt tacccgagcc
     3901 gccatgaccg tcatcggtgg ccagatcgac gagtcctggg cgaacgggag cctcgtcatg
     3961 acccaggagc agtacctaca gcggatcggt aacaagaccg ctgccctctt cgcgctggcc
     4021 gcggaggggg gtgcgatgct cagtgaggct tcccagccgg tgatcgaggc ggtgaagcag
     4081 tacggctacc tgatggggtt ggccttccag atcgtggacg atatcctgga cgtggtcggg
     4141 gacgagggaa gcctcggcaa gccggtgggg agcgacgtga ggcagggaac cgtgacgctg
     4201 ccggccctga tggtgcgcga tcgcctcacc cccgagcagt tcaaggcggc cttcaacggg
     4261 gatggacacc gggaagggcc gataaacctg ctgctggata cgatccggcg agagggcggt
     4321 attgagctgt cctacaggga ggccgacaga ctggcggccg atgctcgcgc ggcgctgaag
     4381 ccgttgccgg ccggcgaggt ccgcgactcc ctggaagatc tggcgcgctt cgtcgtccgc
     4441 cgcaacgtct gatggagtaa tttgatgaag gtaaccttcc cccacgccgg gcaggcgtgg
     4501 gttcccctgt ctaccgtctt cggcaaggct ggggttgagt gcatagttcc cccgcccacc
     4561 agcaagcgca ccctcgccct cggcgtcaag tactctccgg agtgggtctg cctgcccttc
     4621 aagatgaacc ttgggaactt catcgaggcg atggagatgg gcgccgacac cctggcctac
     4681 gtctcgggct cggggctctg ccgcctcgga tactactcca aggtctcgga tcagattttg
     4741 cgggacgccg gctatgagtt cgagatggtt accttcgact ggcaggagac gggggtcatg
     4801 gggttggcga atttctttcg cagggtgctg cccgatcgtc ctctgcgaca gatcgccggt
     4861 gacataaagt ttggcctctc gcagttgaag acgatggacg acctcgagcg aatgactcac
     4921 cggctgcgac cccgagcgga caaccccgtg gagatcactc ggatctggcg gacggcgggg
     4981 gagaggatcg cccgggcatg gtcgcctgcc gatctgaagc gggccagggc cgaattggtg
     5041 gacgagttca actcggtgcc gctgaacccc tacgccgacc ctctgcgggt tgggttattg
     5101 ggggagttct tcatggtcgt cgagtccttc gtgaatatgg atgtggagga tcggcttggc
     5161 cggatggggg tggaggtcca ccgtcacgca tacttcgggg actgggctcg cacctggctc
     5221 ttcatggagg ccatcgggct cagccacggc aagaaggtga agaaggcggc ggcgccctac
     5281 ctcagccgcg acgtcagcgg cgacgccgtg cagaccgtgg gcgagactat cctccaccag
     5341 caggagggct tcgacgggat cctgcatcta ctgcccttca cctgcatgcc cgagatcatc
     5401 gcccagaacg ttatgccgac cgttaccagg gatttcagta tcccggttct ctccatcgtg
     5461 ctggacgagc agttggggca ggcgggattc ataacccgga tcgaagcgtt cgtggatctg
     5521 atgaaccggc ggcggacggt caagcgagca agctgaaccc gatttggagt gcggcgcgtg
     5581 agcgccactt tggtgggggt ggcgtaagtc cccccgcgct caagcctcgg atgcgcaaat
     5641 gaaatagagg ctttggccgg aacggtcttc taccggccaa agcctctatt ctgtttatgg
     5701 tcaatcaggt ggggtttcgt ggggcgcgcg gtagcggcag acggttcgga gggttcggta
     5761 cagggccgtc agctctgagc tgtggcctgg tctcgagaga gcaggctccg ggcgtcctcg
     5821 cccacgccca actcgatgca gtcacagtag gtggagtaga ttgccctgct cagcagcttt
     5881 gcccaccagt cgttggacgc ggacagcgcg gcgtgctcgc gacgcacacg cagcagtcgc
     5941 tcaagtcgca ggaggaagaa cagctgaacc tcgctgtagg gcttgatcct gccctgtcgt
     6001 ccaatcctcc gatcccctat tgagttcaaa tcccaagaat caacgccact catctcagtt
     6061 ccccccagca ggtcgctgcc ggttctggcc ttccctctag gagtccacgg ccaggcatgt
     6121 catagtcttg ctgatgcccg gtatgcctcg aacttcattc agcaccagcc gtcctatgac
     6181 attcgcgtcg gcgccctggg ccacggcgat cacgtcgtac ggcccggtca ctacatcgga
     6241 ggccgcgatg ccggacacct ttcgcagcgc gtccgccacc tctttgctct tgccccccaa
     6301 cgtttcgatc agaacgtatg ctctttgcat cgccgtttca cctccactgc agcgctcgat
     6361 tctgcgagcc ggtagctatc gctaccatcc tcatattagg ctttcctgcg gcgatgtcaa
     6421 gacctttcgg ccctccccag gagtgcactt ggcgtgcctt gtgcctccat ttccccgcaa
     6481 cccaggctac aaggtactgg cacactaccg aagagttgcc cacagcctat cacgattcta
     6541 tgatgcttac agcagctaat tagcagcagt cgaggtaggc gacgataacc gatctcttat
     6601 ccctcatttt gcccccgtcc gccgtctgag ggttgccgcc tccaggatgc cggagggatc
     6661 ctccatcttc tccatgtgag ccacctgctc cgcaagctgc tcggccctgt ccctgggcag
     6721 tattcgggtc gatagctcca gatacttctc catcaattga tcgtcgggca taggattgtt
     6781 gggggttccc gtggcggcct ccactcgctc caccagtatc cggccgtcca ccgttcggat
     6841 cgagaccacg gcctggtcct ccagcacgcc cggctccact tgggccctca ccctgtccat
     6901 caacgcaatc acgtccgggc ggttcacctt ctcgtcggtg aactgggact cgcgtgcgtt
     6961 gccctctatc agagcgatgg cagcgcagaa gtagatgctg aacttgccct ccaacccggt
     7021 gcgaggggac ttcttgccgg tcagttccct caccagtggg tgaatcttcg tctctatctc
     7081 cagcacgtcg ttcggtgcga gccggtatcg ctccctcagc cttcgcacgc cgtcgatggt
     7141 cgggtgggtc acgacgccgc aggcgtatgg cttgatcccg ttctgcagca tcaggtactc
     7201 gtcgcccaac cccacggtga tctgtcccag gtcgtactcc gatgcggtca ccgcgcagaa
     7261 gcccctgcgc ccctccagca cctcggtgga ggcggtgaac ccccgggtgg ccagcatcgc
     7321 cgagaggagg ccgtttgagg ccgcctttcc cacgtgcagc gccttggtca tggatccgaa
     7381 catctcgcgg agcccggccg cctgggctcc ggccatcccc agcgcgtgcg tcatctgctc
     7441 ctcactcagt tgtagcagct ttcccaccgc cgcggcagca ccgaaggtgc cggcggtgcc
     7501 ggtgatgtgc cagcccacgt cgtagtgagc ggggtagact gccgatccca cccttagctc
     7561 gacctccatt ccggtggcca acgcggcgat caactctcgc ccgctcagcc ctttccactc
     7621 ggccgtggcc agggccgcgg aaagcaccgg gcctgtgggg tgaatgacgg tgggcagatg
     7681 ggtgtcgtcg taatccagca cgtgagacat ggttccgtta atcgtggcgg cctggatcgc
     7741 cgagcagcgc ctcccgtgtc ccagaacggt agcctgacgc tttcccccct cctcggcgat
     7801 cgtctccatc agcagccgaa ctgcctggtg atcggcagcc cccagcgcca ctcccaggta
     7861 gttcagtatg caccgtttgg actctcggac gacctctgtg gggagcctat cgaaggttgt
     7921 gcggaccgca aactctgaaa gactgcgggt tgcgcccatg gatggagcct ccctatatac
     7981 attcaattcg gcacgacccc gcctcggcag tagggggtct gcgacctact tgtggacgag
     8041 actgttacgg gtggcatcag acgaaccgat actactataa tagaaaaccc cgaccgctct
     8101 acggtcgcgt ggcgtggctt ttcgctggcc cttatggtaa actcgcgcgt cacttcagtc
     8161 gttgtggcgg tggaggttgt ccctcatgaa gcagcaggtg aatgcctttc tcggatacct
     8221 ggcagccgag agaggtttgg cggcaaatac gctggccgct tacaataacg atctcagcca
     8281 gttcgcagaa tacttagctt ctcgtgatca ggctgggatc gaagggtccg gttccgaggg
     8341 cagccatcag gatggtcagg ggtcgctgtc ggtcatgggc agggacgccg tgatgggctt
     8401 ctttctccat cttcgagaga agggctacac accctctacc atagccagga agaccgccgc
     8461 tatcaagtcc ttttttcact ttctcgcctc caagggctac gtttctgagg accccaccgc
     8521 cagcatcgat tccccccgga tcagcaagtc gttgccgcgg gccgcatccg tggaggagat
     8581 agacgaactg ctggaggccc cgaccaggat ttcgtccccc gaggcactca gggataaggc
     8641 gatgttcgag ttgctgtacg ccacgggcat gcgggtcacc gaactggtct ctctgaatct
     8701 gggcgacgtg gacgtggttc agggctgcgt acagtgctcc ggccgcagcg ccggcaaggg
     8761 tgccaggctg cgggtgatcc cggtgcacgc cacagcgatc aaggcagtcc aggagtatct
     8821 ggagaaggcg aggccgatgc tggtgcgtga gagcgacgag atggcgctct tcgtcaacca
     8881 tcgcggcgac cgcctgaccc gccagggctt ctggctgatc ctgaagcagt gggccaagga
     8941 ggcgggcgtc accactcaga tcacccccca cacgctgcgc cactccttcg cgctgcacat
     9001 gctgaagaac ggcgtggacc ttcgcgcggt ccagcagtta ctgggacacg cccacatctc
     9061 caccacccag atatacacct atcccagcga agacggcctc aagcgcctct acgaaaccgc
     9121 ccatccccgg gcaaagtaga tggtcggggc tgaagagcgc ccgacagggg agcccactcc
     9181 gacttgccgc gccgtcctgt tcgacgctgg caacaccctc atctacatgc cccagacggc
     9241 cgaggatatc ctgatggcgc tctgccatca gttgggcgcc cccatcgacc tggaggccgc
     9301 ccgcggcgct taccaccgat ccgagagcta ttacgctcgc aactatctgg gctactccgg
     9361 cgagcagggc gccttctggc atgcgtacca cggcgaggcg ctccgctatc tcgggatcga
     9421 cgaccccgac ggacggaagg cggccttcct ctcccactcc ttcggacggg ccggcgtatg
     9481 gcaggcttac cccgaggccg agcgggtttg ctccacgctc cgggccatgg gtgtgcgact
     9541 gggggtcgtc tccaacggcc ccgtcactgt ctcggacctt ctcactcagg ccggcctgat
     9601 ccccttcttc gagaccgtga tcgcctccca ggccatgggt atccagaagc cggacccgcg
     9661 gatctttcgg gaggcgctgg ggaggatggg gcttcgcccg aacgatgcgc tgttcggtgg
     9721 ggacctttat gatgtggacg tggtgggcgc ccgctccgcc ggtctgcgag cggttctgat
     9781 cgatcgcgac ggccacgaag gcgagagcga ctgccccacg atccgcaccc tggacgagtt
     9841 gatcccgctg gttaccatgt ttggagcacg ccatgtctga cctctcgaga gccgtagagt
     9901 tggtctcctc catcgccacg atccggccga gggtcgggat cgtgctcggc tccggtctcg
     9961 ggatggcggc cgaggggatg gaggatgcca tctccattcc ctataggaac atccccggct
    10021 ttccctccac caccgttggt ggccatcgtg gcgagctggt gctgggcaca ttggaggggc
    10081 tgggggtcgc ggcgctgatg ggtcggccgc acctctacga ggggtacgcc ccggctcagc
    10141 tgggcttttc ggtccggctg ttgggcgcgc tgggcgtcga gacgctggtg gtcaccaacg
    10201 ccgccggagg cctggcaccc aacctggagc ccgggacgct gatgctgatc gaggatcacg
    10261 tgaacctgcc gggcatgggc ggccacaacc ccctggtggg tatcgagggc ggcgatgagc
    10321 gcttcgtgcc gatgaccggc gcatacgacg agacgctgcg ccgtgtggct caggatgccg
    10381 cggcggaggc cgggatccgg ctggcgacgg gcgtgtacgt gatggtgtcc ggccccaact
    10441 acgaaaccgc cgcggaggcg cgcttcttgc gccttctcgg ggcagacgcg gtggggatgt
    10501 ccacggtgcc cgaggtgctg gtcgcgcgat ggctggggat gcgggttctg ggcatatcct
    10561 gcatcacgaa cctgctgctc ggcccgccca gccacgccgt cgacggccac gccggggtcc
    10621 tctccgcggc cgcgatggcg gcgcccggca tggcgcggct gattcgatcg gtcgtgcgcc
    10681 acctggcccg tcaatagatc ccctcccagg tttccggttc gaaatcgatt ctacgggggc
    10741 agtatcctga ggtgaagatt tactccttcc tgctaccgct catgctcgtg gcgagcaccc
    10801 tcatcgggtt tggctaccag ggaacgaccg ccgctgcccc ccctgcacct gcccaggaga
    10861 cgatccctca gactcaacta ccgtggcggg atctggcgga tctggccgtt cggctaaaag
    10921 gggtatcgcc cgacgcggtg aacggggttc caccgcagga tgttcccgag aagcaggtgg
    10981 gggaccagac cagcttctgg gtcgcggatc aggccaacaa ccgttactac tccgtggatg
    11041 ccaccctgaa ggtggtaacc ccccacgcct acatgtacgt ggccgatgac tccatgctgg
    11101 acattcccag gctgcaggag gcggcccagg ggttcgagga tcgggtctac acagtcgatc
    11161 gccactactt cggcagcgag ccggcggtgg ggctggacgg cgatgcccac gtctctatcc
    11221 tgcacgccac catacccgga cttggtggct acttcacctc cgtggacgac tatccccgtc
    11281 ccgtgcaggg ctacagcaac gagcgcaagt cgatttacat aaacgttcag gtggcgccgc
    11341 ccggctcgct gggctactac gccatcctcg cccacgagtt cgagcacatg atccactgga
    11401 acaccaaccg tgaggagcag acctggatca aggagggggc cgccgaggag gccacggagg
    11461 tgaccgacct gggcggcggc agttccgtgc gggacttcga gatcgccccg gacacccagc
    11521 tgaatgcctg gtcg
//
LOCUS       DATALS010000378         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_266076, whole genome shotgun sequence.
ACCESSION   DATALS010000378 DATALS010000000
VERSION     DATALS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266076"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1499)
                     /locus_tag="VHS06_08510"
     CDS             complement(6..1499)
                     /locus_tag="VHS06_08510"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX2988195.1"
                     /translation="MTAEQRLHAQSLSSPVTWTRPKVHPCTQYRLLRGALIAMDAAAL
                     ALAIGLAYLIRFKTGVPFLETPPHTVAFYSSVAFWSVPAWLATFALYRLYDRHYLFTG
                     FQEYARVINGCTVGMVIVIFVSFIDIGLYISRGWLLIVWATSVALVGGFRFAFRRVLR
                     RVRSAGFLTVPTVIVGANEEGRALAEQFLGDPGAGIRILGFIGSSCSCDPPKDGPFPI
                     LGGLNDLERVVQRWEVGEIIVAPTALTRDELLDLYRSLGHSESVEIRLSSGLFEILTT
                     GVSVQEISCVPLVTPQRVRITGVDAVLKAVLDYTGALVGLILLSPVMLAVAILVKLDS
                     PGPVLYRRRVLGRFSKPFDAFKFRTMVVDADNVLESNALLKETFSSRYKLKSDPRVTR
                     IGRILRRTSLDELPQLFNVLRGEMSLVGPRMISPDESGRYGKWQLNLLTVRPGITGPW
                     QVRGRSEIPYEERVQLSMHYIRNFSIWLDLEILLRTIPAVLKARGAY"
     gene            complement(1574..3163)
                     /locus_tag="VHS06_08515"
     CDS             complement(1574..3163)
                     /locus_tag="VHS06_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2988196.1"
                     /translation="MDGVVDQTAEDRLIDSICYNRGTRDLRKLTVPDYPDAGPTIRAA
                     NGKQDHRVDVLLANPPSPDGAIWIRSQHRVGRRSRENMIWPQVSLAQLAALVPPGLSV
                     EIVDAIATRMDWSQFERMLREKRPRLYLTQVTGPTLTNDMYGVFLAKSLGAWTIGFGT
                     HVTPMPRETMEPFPCLDFVIRGEPEYTFKELVETLSGESRGCQGDLELPDIAGLVWRR
                     GKDIVVNPDRPFISDLDALPLPLHEMLPLDRYRVPMFEGPFTFIVTSRGCPAGCSFCI
                     KHVSYQYSVRVRSPESIQQELWVLNRLGIHNVHMYADLFTANREQVVGLCELLLREGP
                     KVRWTCNSRVDHVDEEMLRLMGRAGCWFISWGIESASKEILHRARKGTDPEMVERALQ
                     WARAAGIKNWGYFIIGLPGETEETIRQTIQFSKKLPLDLALFHVAAPHPGTPFFFEVM
                     ANNWFRPGTRWEEVDMDRSTVLDYPHLSAEELERWQKKAFLEWAMRPGPMATYLQMLA
                     TPGALRSALEVGIRHLSWARS"
     gene            complement(3339..>4701)
                     /locus_tag="VHS06_08520"
     CDS             complement(3339..>4701)
                     /locus_tag="VHS06_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2988197.1"
                     /translation="GWKLSEKGVEGEVVESHSYVGGYSHTIQRDGYLFDLGPHRFHTD
                     NPAVLAEVERLTGKLPQLVRKTRVYFMGNYFDYPLTAGNLLSSLPPTLGILCFGDFLT
                     TWLRNRVWPTPDDSFESWVVNRFGRRLYDIYFGPYTAKVWGRDPSRLSASWAAQRVAV
                     VDLWDLVTRLLGFRRGYNNFHHSPYKLEFQYPRMGVGYIYQRMAEEILRQGGVVSLDS
                     TVREVTHSDGHVQGVIVERDGSLKELAGDYFVSTIPIPDLVRALRPCPPDDVLAAAET
                     LKYRAMVFLFLTIDRESVTDDHWIYFPEGDVVFNRISEMKNFTPDAAPQGKTSLTVEI
                     SCEVNDEVWNTPDALLYDRSIQGLEGAGLIRADQVIDHFFVRASHAYPTYHQNFEVAL
                     GRLAYHLAGLDNLIVCGRQGLFRYINQDHAIEMGLCAAEDVLTQPTASRVGRVGAENV
                     YFG"
BASE COUNT         1022 a         1439 c         1340 g          900 t
ORIGIN      
        1 gtcgcttaat aggcgcctct ggcctttaga accgcgggaa tggtgcggag cagtatctcc
       61 agatccagcc aaatgctgaa gttgcggatg tagtgcatgc tgagctgaac cctttcctcg
      121 tacgggatct cgcttcggcc ccgcacctgc cacgggcccg tgatccccgg cctaaccgtg
      181 agcagattga gctgccattt gccatagcgc ccggactcat ccgggctgat cattcgtggg
      241 cctacgaggc tcatctcacc acgcagcacg ttgaacagct ggggaagctc gtccaggctg
      301 gttcgacgaa ggattcgacc aatcctcgta actcgagggt cggatttcag tttgtagcga
      361 ctgctgaagg tctcttttag cagggcattt gactccagta cgttgtccgc atccaccacc
      421 atagtcctga acttgaacgc atcgaacggc ttggagaagc ggccgaggac ccgacgtcga
      481 tatagcacag ggccgggcga atccagcttt acgaggatcg ccactgccag catcaccgga
      541 ctcaatagga tcaatccaac gagtgcaccc gtgtaatcca gaaccgcttt cagaaccgca
      601 tccacgcccg tgatgcggac acgctgcgga gtaaccaagg gcacacaact gatctcctgc
      661 acacttaccc ctgtagtcaa gatctcgaaa agccccgacg aaaggcggat ctccacactc
      721 tcggagtgtc cgagagagcg gtacaggtcc agcagttcgt cccgcgtcaa ggccgtgggc
      781 gcgacaatga tctcgcccac ctcccagcgc tgcaccaccc gctccaggtc gttcagccct
      841 ccaagaatag gaaacgggcc gtctttggga gggtcacacg agcacgagct gccgataaac
      901 ccaaggatcc gaattccggc cccaggatcc cccagaaact gttcggccag tgcccgtccc
      961 tcttcgttcg cccccacaat taccgttggc actgtcaaga agccggcaga gcgcacccga
     1021 cgcagaaccc gccggaaggc aaacctgaac ccgccaacta aggccaccga agtggcccag
     1081 actatcaaca accacccgcg agagatgtac aagcctatgt caatgaaact cacgaagatc
     1141 acgatgacca ttccgacggt gcagccgttg atcactcggg cgtactcttg aaacccggtg
     1201 aagaggtagt gccgatcgta gagtcggtac aacgcaaagg tggccagcca ggccggcacc
     1261 gaccagaatg cgacggacga gtagaacgcc acggtgtgtg gtggggtctc caagaagggg
     1321 acgccggtct tgaagcggat gaggtaggcc aaccctatgg cgagtgctag cgccgcggcg
     1381 tccatagcta tcagggcacc acgcagcagc cgatactgcg tgcaggggtg gaccttaggt
     1441 ctcgtccagg tgactggtga tgaaagggac tgtgcatgaa gacgctgttc cgcggtcatc
     1501 gggccgtccc atctactctc gttagtgtga gagcgacacg ctcagaaaac cggtggaagc
     1561 cgtaccaacg ggatcaactg cgcgcccagg aaaggtgccg gatgcccact tcaagagccg
     1621 atcgcaaggc ccccggggtt gccagcatct ggagatatgt cgccatcggc ccgggcctca
     1681 tcgcccattc cagaaaggct ttcttctgcc accgctccaa ctcctcggcg ctcaagtgcg
     1741 ggtagtcgag cacggtggac ctgtccatat ccacctcttc ccatcgagta cctgggcgga
     1801 accaattgtt ggccatcacc tcgaagaaga acggagtgcc gggatgcgga gccgccacgt
     1861 gaaacagtgc aaggtcgaga ggaagcttct tggagaattg aatcgtctgc cttatcgtct
     1921 cctcggtctc cccaggcagc ccgatgatga aataccccca gttcttgatc cccgccgccc
     1981 tggcccattg gagagcacgt tccaccatct cgggatcggt acccttgcga gcgcggtgca
     2041 ggatctcctt actcgccgac tcgatgcccc aggagatgaa ccagcagccg gcacgaccca
     2101 tcagtctcag catctcctca tcgacgtgat cgacgcgact gttgcaggtc cagcgaacct
     2161 tgggcccctc acgaagcaac aactcacata gccccacgac ctgctcgcga ttcgcggtga
     2221 agagatcggc gtacatgtga acgttgtgga tgcccagccg attgagaacc cacaactcct
     2281 gctggatgct ctccggcgac cgcacacgaa ccgaatactg ataactcacg tgcttgatgc
     2341 agaagctgca tccagccggg cacccccgac tggtgactat gaaggtgaat ggcccctcga
     2401 acattggaac ccggtaccgg tccaagggga gcatctcgtg cagcggcagt gggagggcat
     2461 cgaggtccga gatgaaggga cggtctggat tgacaacgat atccttgcct ctgcgccaca
     2521 cgaggcccgc gatgtccggc aactccaagt ctccctgaca accacgtgac tcgccggaga
     2581 gagtctccac caactccttg aaggtatact caggctcacc tcggatcacg aagtcgagac
     2641 atgggaaggg ttccatcgtt tcccgaggca tcggcgtcac gtgggttccg aatccgatcg
     2701 tccaggctcc aaggctcttg gccaggaaga ccccgtacat gtcgttcgtg agcgtgggcc
     2761 ccgtgacttg agtgagatac aggcgtggcc tcttctcgcg caacatccgt tcaaactggg
     2821 accagtccat gcgggtggca atggcatcga cgatctctac cgacagtcct ggtgggacga
     2881 gtgcggccag ctgagctaag gaaacctggg gccagatcat gttctcgcgg gatcttcgcc
     2941 ccacccgatg ctggctgcga atccagatcg cgccgtcggg cgacgggggg ttcgccagta
     3001 gcacgtcgac tctgtgatct tgcttcccgt tggccgcccg tatcgtcgga ccggcatctg
     3061 gataatcggg tacagtcagc tttcgcaagt cgcgagtgcc acggttgtag cagatgctgt
     3121 ctatcaatcg gtcctctgcc gtctgatcca caactccatc caattgggga ctcctcctga
     3181 gatttccaaa gcactagggt ctaagtacgt cgtagaaaga aacgggtcca gggcacgcga
     3241 cggtagcgag cgtggcgtcg cggcttggcg gggcgcaacg ctgcgctcgc tcgcgttgcg
     3301 gggtgtcccc aaagttgctg tttctttcta ctacctacct agccgaagta gacgttctcg
     3361 gcaccgacgc ggccgacccg actcgccgtc ggctgggtca gcacatcttc cgccgcgcag
     3421 agccccatct ctatcgcatg gtcctgattg atgtatcgga acagcccttg acggccgcag
     3481 actatcaggt tgtccagccc cgccaggtgg tatgcgagtc tgcccagggc aacctcgaag
     3541 ttctggtgat aggtagggta ggcatggctg gcccgtacga agaagtggtc gatcacctga
     3601 tcggctctta tgaggcccgc gccctcaagt ccttgaatcg agcgatcgta gaggagcgcg
     3661 tccggggtgt tccagacctc gtcgttcacc tcgcacgata tttcgacggt cagcgaggtc
     3721 ttcccctgag gcgcggcgtc cggcgtgaag ttcttcattt ccgagatacg gttgaacacc
     3781 acgtcaccct cagggaagta aatccagtga tcgtccgtaa ctgactcgcg gtcgatggtc
     3841 agaaagagga agaccatcgc cctgtacttc agcgtctctg cggctgccag cacatcgtca
     3901 ggcgggcatg gtctgagcgc tctcaccagg tcgggaattg gaattgtcga gacaaagtaa
     3961 tcgccggcaa gctccttgag actgccatcg cgctccacaa tcacgccctg aacatggcca
     4021 tcactgtggg taacctcgcg cacggtggag tccagggata cgacacctcc ttgccgaagg
     4081 atctcttcag ccatccgttg gtagatgtac cccaccccca tacgagggta ttgaaactcg
     4141 agcttatagg gtgagtgatg gaagttgttg tagccccggc ggaatcctag tagacgcgtg
     4201 accaggtccc aaaggtcgac cacggcaacg cgctgggcag cccaacttgc ggagagcctg
     4261 gatgggtccc tgccccaaac cttggcggta tacgggccaa aatagatgtc gtaaagtcga
     4321 cggccaaacc gattgacgac ccaggactcg aaggaatcgt cgggagtagg ccacacccga
     4381 ttcctcagcc aggtggtgag aaagtcaccg aagcagagaa tccctaaggt cggcggaagg
     4441 cttgacagca gattacctgc ggtgagcgga tagtcgaagt agttccccat gaaatagact
     4501 cgcgtcttcc gaaccaactg gggcagcttg ccggtcaacc gctcaacctc ggccaaaact
     4561 gccggattat ctgtatggaa tcggtgcggg ccgagatcga agagataccc gtctctctgg
     4621 atggtatgcg aatacccacc tacgtaggaa tgggactcga ctacctctcc ctcaacccct
     4681 ttctcactga gtttccatcc c
//
LOCUS       DATALS010000379         4560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_42014,
            whole genome shotgun sequence.
ACCESSION   DATALS010000379 DATALS010000000
VERSION     DATALS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42014"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VHS06_08525"
     CDS             <1..1072
                     /locus_tag="VHS06_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2988198.1"
                     /translation="ASGAKVLHIPTVFGSVVLAYNLPDVKELKLDSDTLASIYLGEIT
                     KWNDPKIAALNSGVTLPDKPIQVVHRSDSSGTTNIFTSYLAGVSPTWSSKVGKGKETM
                     TEPDKKHILLVEDDRTISDLLAYNLRHEGYDVAQEYNGRAGLETALRQPVDLVLMDIM
                     LPGLDGLAASREIIRRKPNLPIIMLTARSDRETVLEGFGLGADDYITKPFDLDVLLAR
                     IQARLRRAPSGAVVRAVAGPATGAVGPSRSVTVADLVLDRDAHTIRGPKGEMGLNPKE
                     YDLLELLLSRPGHLFQRDEIIERVWHHQYAPASRSLDVHIRRLRAKLESIDAGVELQT
                     VRGVGHRVVPGAVVRQNAEDGS"
     gene            1084..2886
                     /locus_tag="VHS06_08530"
     CDS             1084..2886
                     /locus_tag="VHS06_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988199.1"
                     /translation="MRLRYKLPIAFALTTLVFAGIVGLAAAVLLRGVYLDRLENDMSL
                     QARQFASVLEQEAAASVAQDPAYLESLTNQAGDAANLRLTLIAHDGTVLGDSEVDPKA
                     LGNHSDRPEVRQALAGHEGRARRESATLKQQEVYVAVPLPASSASWSSGVVRTALPAS
                     RVDSMVSSSWRIPLVVWAILLVPTLVVSYLLARSITRPVYRMREMTGHVAAGELAYRT
                     GIRRNDELGELATSLNDMASELETHVDQLAAEKERSAQVLTAMTEGVIVVDAEGRVVR
                     ANPAASRILQVPLEDFEGSSLVVAVRAFPGRLLAEKAQRAGVPISEIIELPGQRLVHA
                     EVIPLHTGTGTGGVSGHALFVIRDETARLTLDRTRRDFVSNVSHELKTPLASLSLLAS
                     TLKRAMRNDPDQAGVFLDRLSSEVGRLTDLTDDLLVLSELEEEGTELEREFTPVDVGV
                     LARETVAELLPMAERKEQEVVVESEPDAMVLGDEVTLHTLVRNLLDNAIRYTEPRGHI
                     AVRVWIESDEAGGGRWVVLTVKDDGVGIPAAEQGRIFERFYRVDKGRSRETGGTGLGL
                     SIVRHVAQRHGGAVDVVSTLGSGSTFRVRIPAAG"
     gene            complement(2910..4337)
                     /locus_tag="VHS06_08535"
     CDS             complement(2910..4337)
                     /locus_tag="VHS06_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX2988200.1"
                     /translation="MKRSLPILFCAILATAVLVALEAGCGTVAVGQADDSRSLEGKTW
                     RATVIAGLTKVQTAKGAAATVQFSAGQAGGSGSVNYWGASYTTGPGNAIQISGLITTA
                     MAVAGPQDRMDQEAAYYAALPKATTYNVTETSLTLFDSKGGILVTYEALPPTPLIGTE
                     WQIVGYQWGHPGDETVSSDDPVITAIFGADGTLSGSGEINSYSTKYTTAANGAMTIDP
                     QIISTKMAGPDNYMGQESAYLAALPKTATYAIYGDQLTLRDASGAELAQFTAKSSPTP
                     PTMDSSPRSGNTSPTENPSSTSTDNPSTTVTTGPTTAETEEYAVYSAVIHERFGGSEM
                     IVVSDHTSGGLGNDWQRTVDALPRNLPGCQRETLESFKKNNQHSTALKQELELRQPYV
                     FISNEELEQIFASSDGWDRFYELYPGAQGEMTLSRVGFNATFDQALLYVGNQWHWVAG
                     EGYYLLCTKTDEHWKVSAEAMMWVS"
BASE COUNT          830 a         1371 c         1463 g          896 t
ORIGIN      
        1 tgccagcggg gcgaaagtgc ttcacatccc cacggtcttc gggtctgtcg tgttggcgta
       61 taacctaccg gacgtcaaag agctaaagct agactccgac actctcgcct cgatctacct
      121 tggcgagatc accaagtgga acgacccgaa gatagcggcc ttgaactcgg gggtcaccct
      181 gccggataag cccatccaag tcgtgcatcg ctctgactcc tcgggtacca ccaacatctt
      241 caccagctat ctcgccggcg tcagcccgac ctggtccagc aaagtgggta aaggcaagga
      301 gaccatgact gagccggaca agaagcacat cctgctggtc gaggacgacc ggacgatctc
      361 ggatctgctc gcctacaacc tgcgacatga gggttacgac gtagcccagg agtacaacgg
      421 gcgggcgggg ctggaaaccg ctcttcgcca gccggtcgat ttagtcctca tggacatcat
      481 gcttcctggg ctggatgggc ttgcggcgag cagggagata attcgccgca agcccaacct
      541 tccgataatc atgttgaccg cccgcagcga ccgagagaca gttctcgaag ggttcggact
      601 gggggccgat gactacatca ccaagccctt cgaccttgat gtgctcctgg cccggattca
      661 ggcccgcctc agacgggcgc cgtccggcgc cgtggttcga gcggttgcag gcccggctac
      721 cggcgcggtc ggccctagtc gaagtgtgac ggttgccgat ctagtcctcg atcgtgatgc
      781 ccacaccatc cgggggccca agggtgagat ggggctgaat cccaaggaat acgacttgct
      841 tgaacttctg ctctcccgtc ccggccacct gtttcagagg gacgagataa tcgagcgagt
      901 gtggcaccac caatatgcgc cggcgtcccg tagcctggat gtccacatcc gccgactacg
      961 tgcgaagctc gagtctatcg acgcgggcgt ggagttgcag accgtgcgcg gggtaggcca
     1021 ccgcgtcgtg cctggcgcgg tggtccgaca aaacgctgaa gacgggtcgt gaggggctga
     1081 agggtgcgac tccgctacaa gcttcccata gcgttcgcgc tcacgacatt ggtgttcgcc
     1141 ggcattgtcg gcctcgccgc cgcagtcctg ctgcgcggcg tctatcttga ccggctggag
     1201 aacgatatgt ccctgcaggc ccggcaattc gcctcggtgc tggagcagga agcggctgct
     1261 tcggtcgcac aggatcccgc ctatcttgag tccctgacca accaggcggg tgacgccgcc
     1321 aatctgcggc tcacccttat tgcacatgac ggaacagtcc ttggcgactc ggaggtagac
     1381 ccaaaggcac ttggcaatca ctcggatcgc ccggaggtgc gccaagcgct cgccggtcac
     1441 gagggccggg cgcggaggga atccgcgacg ctgaagcagc aggaggtata cgtcgcagtg
     1501 cccctgcccg caagcagtgc ctcctggtcc tccggcgtcg ttcggactgc tctcccggcc
     1561 agtcgggtgg acagtatggt gtcctcctcg tggaggatac ccctcgtagt ctgggccata
     1621 ctgctcgtac ccacgcttgt cgtctcctac ctcctagccc ggtcgatcac ccggccggtt
     1681 tatagaatgc gagaaatgac gggccacgtg gccgcgggcg agctggcata ccggacaggg
     1741 atccgccgaa acgatgagct cggggaactc gctacctcgc tcaacgatat ggcgtccgag
     1801 ctcgagactc atgtcgacca gttggctgcg gagaaggaga ggtccgccca ggttctcacc
     1861 gccatgaccg aaggagtcat agtcgtcgat gccgaagggc gggtcgttcg ggccaacccg
     1921 gcggccagcc gcatcctgca agttcctctc gaggactttg aggggtcttc gcttgtcgtc
     1981 gctgttcggg catttcccgg acgcttgctc gcagaaaagg ctcagcgagc tggcgtgcct
     2041 atcagcgaga tcatagagtt gccaggacag cggctcgtgc atgcggaggt aataccgctt
     2101 cacacgggaa ccggaacggg aggcgtgtcg ggccacgctc tattcgtaat cagggacgag
     2161 actgcgcggc ttacacttga tcgaacccgg cgggacttcg tgagcaatgt atcccacgag
     2221 ctgaagaccc cgcttgccag cctctccctc cttgcctcta ctttgaagcg tgcgatgagg
     2281 aacgatcccg accaggcggg cgtgttcctc gaccggctct cctctgaagt aggacgcttg
     2341 acggacctta cggacgacct cctcgtgctc tcggaactcg aagaagaggg gaccgagtta
     2401 gagcgggagt tcactccggt cgacgttggc gtgttggcca gggaaacggt ggcggagttg
     2461 ctgccgatgg cggagcgaaa ggaacaggag gtcgtcgttg agtcagagcc ggacgcgatg
     2521 gtgcttggcg acgaggtgac tctacacacc cttgttcgga acctgctcga caacgcgatt
     2581 cggtataccg aaccccgagg ccacatagcc gtacgagtat ggatcgagag cgatgaggca
     2641 gggggcgggc gttgggtggt gctaacggtg aaggatgatg gagtgggcat tcccgctgcc
     2701 gaacaagggc gcatcttcga aaggttctat cgggtcgaca agggtcgttc tcgagagact
     2761 ggggggacgg gcctaggcct gagcatcgtg cgccacgtcg cgcagcggca cgggggcgcg
     2821 gtggatgtag tgagcacatt aggatccgga tctacgttca gagttcggat cccggccgct
     2881 ggatagcggc gagcgccagc gtcacccgcc tacgagaccc acatcattgc ctcggccgat
     2941 accttccagt gctcatccgt cttggtgcac agaaggtagt agccctctcc ggctacccaa
     3001 tgccactgat tgccgacgta caacagcgct tggtcgaatg tggcattgaa tcctacgcgt
     3061 gacagggtca tctctccctg cgccccgggg tacagttcat agaagcggtc ccatccgtcc
     3121 gaggaagcga atatctgctc caattcctcg ttgctgatga acacatacgg ttggcggagc
     3181 tccaattctt gcttcaaagc tgttgaatgc tggttgttct tcttaaagct ttcgagcgtc
     3241 tcgcgttgac agccggggag atttcggggg agagcgtcca cggttcgttg ccagtcgttc
     3301 ccgaggcctc ccgaagtatg atcgctgaca acgatcatct ctgacccccc gaaccgttcg
     3361 tggataaccg cactgtagac cgcgtattct tcggtttcgg ccgtcgtggg tccggtggtt
     3421 accgtagtcg aggggttgtc ggtcgaggtg gaagaggggt tctcggtggg cgaggtattc
     3481 cctgacctcg gggagctgtc catcgttggt ggcgtcgggc tggacttggc cgtgaactga
     3541 gcaagctcgg cgcccgacgc gtcccggagc gtgagttggt caccgtagat cgcgtaggtg
     3601 gccgtcttgg gcaaggccgc caagtaggcg ctctcctgtc ccatatagtt gtcgggtccg
     3661 gccatcttgg tgctgatgat ctgcgggtcg atggtcatgg cgccattcgc ggccgtcgtg
     3721 tacttcgtgg aatagctgtt gatctcgccg ctccccgaga gcgtcccgtc ggcgccgaag
     3781 atagcggtga tgaccggatc gtcggacgag acagtttcgt caccgggatg gccccactgg
     3841 tagcccacaa tctgccactc ggtgccgatg agcggcgtgg gcggcagggc ttcgtacgtc
     3901 acgaggattc ctcccttgct gtcgaagagg gtgagggacg tctcggtgac gttgtaggtg
     3961 gtggccttgg gcagggccgc gtagtaagcg gcttcctggt ccatacgatc ctgagggccg
     4021 gccacggcca tcgccgtggt gatgagcccc gagatctgga tggcgttgcc tgggccggtg
     4081 gtataggaag cgccccagta gttgaccgac cccgacccgc cggcctggcc cgccgagaac
     4141 tggaccgtgg cggcggcacc cttcgccgtc tgcactttgg tcaggcccgc gatgaccgta
     4201 gccctccacg tctttccttc caacgacctg gaatcgtccg cctggcctac ggctacagtc
     4261 ccacatcccg cctccagggc caccagcaca gccgtagcga ggatcgcgca gaagaggatg
     4321 ggcagcgaac gtttcatgtc ttacccccgc agaacatgtc gatgctcggt gcaccctggg
     4381 agccggcacc cgttccggct gtaacgacgc ttctgacagc cgtgcaatgg gagcgggttc
     4441 ctgccggcca tcgtgcgtca gtatgccgat gacatggtga ccaaccctac gacttgaatt
     4501 ggcttttcgt gcaactcgac gtgggtcatg ctcgcggtag ctggcgcgag acctgcggca
//
LOCUS       DATALS010000380         7711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_398197, whole genome shotgun sequence.
ACCESSION   DATALS010000380 DATALS010000000
VERSION     DATALS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398197"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VHS06_08540"
     CDS             <1..695
                     /locus_tag="VHS06_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988201.1"
                     /translation="PRLLGRAEVGDGGWGMGVGGRGGGETRGHGGHGDAGSTKHPALS
                     TQNPELRTQNPSESPWVMTAPLVILAIPSILSGFWGSPLASGGGFAAFLEGHAEASGP
                     NLGVMAASIVVALSGIWLAWLMYGKRSLSPERISAAFGPVYQVLSHRYWVDELYNGFV
                     RVVVLGVAKGLRLFDVHVVDGAVNGVARITGAAGSTLRQFQTGRLQSYGWAMYAGASA
                     IAVVTIAAQLSR"
     gene            971..2422
                     /locus_tag="VHS06_08545"
     CDS             971..2422
                     /locus_tag="VHS06_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NuoM family protein"
                     /protein_id="HEX2988202.1"
                     /translation="MPYLSIIVFLPLAAAAVVLLAPRGWSRWIALAGSLAVFGLSLAA
                     FAAFDRAAPGYQLVEKVNWIPQYGVSYFLAVDGISLPMVVLTGLLTLVAVLASWRQEL
                     RVKAYFALVMLLETAMLGVFTSLDLLLFFLFWELELAPMYLLIGIWGGPRREYAAMKF
                     ILYTVAGSVFMLVGIFALYFAAGRGSFDMTVLAAQQYALPFQVAVFLLLAVGFAVKIP
                     VFPFHTWLPDAHVEAPTAISILLAGVLLKMGGYGLIRIPIGLLPDAARELAPWIAVLA
                     VINVLYGAAVSMVQEDLKKLIAYSSVSHMGYVLLGAAALTGVGLQGAVLQMFTHGTIT
                     GMLFLMVGLVYDRTHTRIIDKLGGLGARMPAIAVLFVVAGLASLGLPGMSGFVAEFLV
                     FIGAFPVWQLATLLAAFGIVITAGYLLWMLQRVFMGPLPAQWSGLGDAGRLELFNVGL
                     LVVAIVGVGIFPSILTDVIRVGVLPVAQRLAGM"
     gene            2557..3993
                     /locus_tag="VHS06_08550"
     CDS             2557..3993
                     /locus_tag="VHS06_08550"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX2988203.1"
                     /translation="MGLDELDIKLLAPELILAGFGSLVIVVDLFLTPRSKRANLYLAL
                     LGMVVAAAAVLPQVGRNTTSFGGTLVVDDFALFFKAAFLLAAALVALSAQSFLERHPG
                     GEGEFYGLLLFSTTGMMLMAGTRELISIYVALELTSISLYLLAGAARRDQKSSEAALK
                     YLLLGALSSAVLLYGMALLYGLSGTTDLSGIAQAVGSGRSPALLLGMSLVAAGFGFKI
                     AAVPFQMWAPDVYEGAPTPVTAFLSVASKAAGFVVLIRVFNVALPGLSPDWVVLFGTL
                     SALTMTLGNVVALTQTNIKRMLAYSSIGHAGYLLMGVAAASASGLSSLMFYMLAYTFT
                     NVAAFTVVILASKAIPDDRIGGYAGLHKRSPLLAFAMAASLLSLAGLPPMVGFFAKFY
                     LFAAAYQAGLVWLVVLGLLTSAVSLYYYSMVIRQMYLAAPSEEAPVRFSVPAGVSLTL
                     SVAAVFALGILSGPVVGFAQVAVAGMVR"
     gene            complement(4197..4580)
                     /locus_tag="VHS06_08555"
     CDS             complement(4197..4580)
                     /locus_tag="VHS06_08555"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2988204.1"
                     /translation="MGGYPDDVARNRRHWEATSDQYQETHFADLPSRLNDTECKWGVW
                     GVSENRLQVLGDVRGKDVLELGCGGAFWSIRLAKQGARPVGLDFSSGQLAYARRLMAI
                     AKVDFPEGVAGITDISPNTVIGLGS"
     gene            complement(5027..6040)
                     /locus_tag="VHS06_08560"
     CDS             complement(5027..6040)
                     /locus_tag="VHS06_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2988205.1"
                     /translation="MKAMVYHKGHDVRLEDVPIPTLNEGEALIKVAYTGICGSDVSIF
                     NGKHPRAVAPVIIGHEFSGELVQIKGEAKGLKVGDRVVADPLLDCRVCQACLDGIYNS
                     CLNLKMLGHNADGSFAEYVKTRISHIYKMPDPLPMDIAALAEPTAVSAHALRVSRAHV
                     GDNVVVLGGGPIGILVAQMARITTNRPVSIVEIGEWRLEFARKLGFDPIDAKTVNVRD
                     AIMDRTNGRGADVLFDAAGAAPLTEQMCHFCKVGGQIVIVAMPKEPRPFDFGIVTLKE
                     LDLHGCRAFNYADMETGLQMMADRRIDIESMITQRMPLEQCVEGIELAQKAEASMKIL
                     LKP"
     gene            complement(6217..6966)
                     /locus_tag="VHS06_08565"
     CDS             complement(6217..6966)
                     /locus_tag="VHS06_08565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2988206.1"
                     /translation="MGTLSGQTAIVAGASSGMGRATALTLAKAGCRVFAAARKEESLK
                     ELAAEAAAAGCELRFRKADASKRAEVDALIQEAVDCFGKIDVLVNSVGTNTPDRKLSV
                     LSDENWHHLVDTNLNGAYYLTQAILPQFRKQGGGLLIHITSVSGRFTDYSGAAYQAAK
                     AGVNALAHATMLEERANGVRVSIIMPGLCDTPIMLKRAVQPSRETLDKALTPQDIADA
                     CLFLSSLPARTYVPEMIVMPNGLQCLGQTHA"
     gene            complement(7292..>7711)
                     /locus_tag="VHS06_08570"
     CDS             complement(7292..>7711)
                     /locus_tag="VHS06_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2988207.1"
                     /translation="DLAKRLGFDPIDPKKVDTVEEVLRRTGGKGADVLFDTAGAAPVA
                     AQLAKLTKVHGQVMIVAMPKELHPVDLAAFALKEIDLRGCRVYNADDYRAAINLIAEG
                     KIDVKSMISHVLPLEQGVEGLELARKGDASMKILLKP"
BASE COUNT         1250 a         2445 c         2514 g         1502 t
ORIGIN      
        1 ttcctcgcct tctggggcga gccgaggtgg gggatggggg atgggggatg ggggttggag
       61 gtcggggggg cggggagacg cggggacacg ggggacacgg ggacgcgggg agcaccaagc
      121 acccagcact cagcactcag aacccagaac tcagaaccca gaacccgtcc gagtcgccct
      181 gggtcatgac agcgccgctg gtgatcctgg cgatccccag catcctgagc ggtttctggg
      241 gctccccgct ggcaagtggg ggcggcttcg ccgccttcct ggagggacat gccgaggcga
      301 gcgggccgaa cctgggggtc atggcggcgt ccatcgtcgt cgcgctgagc gggatctggc
      361 tggcctggct gatgtacggc aagagatcgc tctcccccga gaggatctcc gcggccttcg
      421 gccctgtcta ccaggtcctg agccaccgct actgggtgga tgagctgtac aacgggttcg
      481 ttcgcgtcgt ggtgcttggg gtagcgaagg ggctgcggct gttcgacgtg cacgtagtgg
      541 acggagcggt gaacggtgtc gctcgcatca cgggagccgc cggcagcacg ctgaggcaat
      601 tccagacagg ccggctgcag agctacggct gggcgatgta tgccggcgcc agcgccatcg
      661 cggtggtgac catcgcggcg cagttgagcc gctaacagag ctttggagtg cggcgcgtga
      721 gcaccacaag gtgggacggg ttctgccctg aagttgccga ctggcgctgg caagaccacc
      781 attttggagt gcggcggctt gacgccgcaa ggtggggcgg ggcagcgtag aagtcacaga
      841 tgggactgaa cagattgagc gcaaccccaa gagggaatgg tgtgtcaggc aacattgcgg
      901 cgtcgagccg ccgcactcca aaacgatgac tccggcgtcc ggtagccgca ctccataggg
      961 gagttggtga atgccctatc tatcgataat agtcttcctg ccgctggcgg cggcggcggt
     1021 cgtgttgctc gcccccaggg ggtggagccg atggatcgcg ctggcgggta gcctcgcggt
     1081 cttcgggctc tccctggcgg ccttcgccgc cttcgaccgc gccgcccccg gctaccagct
     1141 ggtggagaag gtcaactgga tcccgcagta cggcgtctcc tacttcctgg cggtggatgg
     1201 catcagtctc cccatggtgg tgctgacggg tctgctcacg ctggtggccg tgctggcctc
     1261 ctggcggcag gaactccggg taaaagccta cttcgcgctg gtgatgctgc tggaaacggc
     1321 catgctgggg gtcttcacct cgctggacct gctactcttc ttcctcttct gggagcttga
     1381 gctggcaccc atgtacctgc tgatcggaat atggggaggc ccgaggcgtg agtacgcggc
     1441 gatgaagttc atcctgtaca ccgtcgccgg gtccgtcttc atgctggttg ggatattcgc
     1501 actctacttc gcggccggaa ggggcagctt cgacatgacg gtgctggccg cgcagcaata
     1561 cgcgctgccc ttccaggtgg cggtgttcct gctgctggcc gtcggcttcg ccgtgaagat
     1621 cccggtattc cccttccaca cctggctgcc ggacgcccac gtcgaggccc ccaccgccat
     1681 cagcatcctg ctggcgggcg tgctgctgaa gatgggtggt tacggtctga tccgcatccc
     1741 catcgggctg ctgcccgatg cggctcggga gctggccccc tggatcgccg tcctggcagt
     1801 catcaacgtg ctgtacgggg ccgccgtctc catggtgcag gaggatctca agaagctgat
     1861 cgcctactcg agcgtgagcc acatgggcta cgtgctgctg ggcgccgcgg ccctcaccgg
     1921 cgtggggctc cagggggcgg tactgcagat gtttacgcac ggcaccatca ccggcatgct
     1981 cttcctgatg gtggggctgg tatacgaccg gacccacaca cggattatcg acaagctggg
     2041 cgggctgggc gcgcgaatgc cggccatcgc cgtgctgttc gtggtggcag ggctggcctc
     2101 cctggggctg cccgggatga gcggcttcgt cgcggagttc ctggtgttca tcggcgcctt
     2161 cccagtttgg cagctggcca cgctgctggc cgccttcggg atagtgatca ccgctggata
     2221 cctgctgtgg atgctgcagc gggtgttcat ggggccgctg cccgcgcagt ggtccgggct
     2281 gggggatgca ggtcgattgg aactgttcaa cgtggggctg ctggttgtcg ccatcgtcgg
     2341 cgtggggata ttccccagca tcctgaccga cgtgatcagg gttggggtgc tgccggtggc
     2401 gcagcgactg gccgggatgt agccaactac agctgtcatg ctgagcgtag cgaagcatct
     2461 ctccgaccac gaggagatcc ttcgctggcg ctcaggatga caccggtgcc ctgaatggca
     2521 aaagaatgcg ctgccggtta gccggatgca ctactaatgg ggttggacga attggacatc
     2581 aagctgcttg cgcctgaact gatactggcc ggcttcggat cgctggttat cgtggtggac
     2641 ctgttcctca cgcccagatc caagcgagcc aacctctacc tggcgctgtt gggcatggtc
     2701 gttgcggccg ccgccgtcct gccgcaggtg ggtcgcaaca ccacctcctt cggcggcacg
     2761 ctggtggtgg acgatttcgc cctgttcttc aaggcggcct tcctgcttgc ggccgcactg
     2821 gtggcgctct ccgcccagtc cttcctggag cgtcaccccg gcggtgaagg cgagttctac
     2881 ggactgctgc tcttctccac caccggcatg atgctgatgg cgggcacgag ggagttgatc
     2941 tccatctacg tggcgctgga gctaaccagc atcagtctct acctgctggc gggcgcagcc
     3001 cggcgagacc agaaatccag cgaggcggcg ctgaaatacc tcttgctggg ggccctctcc
     3061 tcggcggtcc tgctctacgg catggcgctt ctgtacgggc tctccggcac gaccgacctc
     3121 tccggcatag cccaggcggt cgggtccggc aggtcgcccg ccctgctgct ggggatgtcg
     3181 ctggtggcag caggcttcgg gttcaagatc gccgccgtgc ccttccagat gtgggcgccg
     3241 gacgtctacg agggtgcgcc cacgccggtg acggcgttcc tctccgtcgc gtccaaggcc
     3301 gcgggcttcg tcgtgttgat ccgcgtgttc aacgtcgcgc tgccggggct gagtccggat
     3361 tgggtggtcc tcttcggcac cctttccgcc ttgaccatga cgctggggaa cgtagtggcg
     3421 ctgacccaga cgaacatcaa gcgaatgcta gcctactcca gcatcggcca cgcaggctac
     3481 ctgctgatgg gcgtcgccgc cgcatcggct tccggactct ccagcctgat gttctacatg
     3541 ctggcctaca ccttcaccaa cgtcgcggca ttcacggtgg tgatcctggc ctcgaaggcg
     3601 atcccggacg accggatcgg ggggtacgcc ggactgcaca aacggtcgcc gctgctagcc
     3661 ttcgccatgg cagccagcct gctctccctg gccggattgc cgcccatggt cggattcttc
     3721 gccaagttct acctcttcgc cgccgcctat caggccggtc tggtgtggct ggtggtgctg
     3781 ggtctgttga cctcggccgt ctccctctac tactacagca tggtgatccg gcagatgtac
     3841 ctggcggccc catcggagga ggcgccggtg cgcttcagcg tcccagcggg agtctcgctg
     3901 acgctgtcgg tggcggcggt cttcgcgctg gggatactct ctggtccggt ggtcggcttc
     3961 gcgcaggtgg ctgtggcggg tatggtgaga taagatccgc ggccaggccg tgcgatcctg
     4021 acagcgcgga cgtgctgatg atcgtagggc ggggtcttgt gccccgcctc ccacagcggg
     4081 gttgcgacca cgatggtgac tccgcccgcg ctctaggcgg ggcacaagcg gggcgctgtg
     4141 ctcgctcgcg ccccgtgccc tacgacattt cgtgggtggc tccacagtct gagcaatcac
     4201 gagccaagcc caattacagt gttaggcgag atatctgtga tccctgcgac tccctccggg
     4261 aaatccacct tcgcgattgc catgagacgc ctggcgtagg cgagttggcc ggacgagaag
     4321 tccaggccga ccggtctcgc gccctgcttt gccagtcgta tcgaccagaa ggccccgccg
     4381 caaccaagct cgaggacgtc cttcccccgg acgtcgccca gcacctgcag ccggttctcg
     4441 gagacccccc acacgcccca cttgcactcg gtgtcgttca agcggcttgg caggtccgcg
     4501 aagtgggtct cctggtattg atcgctcgtc gcctcccaat ggcgcctgtt cctcgccacg
     4561 tcgtcgggat acccacccat acggcatacc tccacctacc agcccgaagg cagagtagtt
     4621 gttcggcgat ccccggcccc ggggcgggaa gatggactcc ccgtagacag acgtcgccca
     4681 ttctgagggc tgcacccatt cagcgtcaat aatgttgatc gggttcaggt ggagatagta
     4741 gccgcgggga tgtagattgt gggttttccc tatgctggac cgaggtgaag tccggctgtc
     4801 gttttcgaga aagggagtga ggctctccgt ggacgggaac cgggcgggtc agggacgacc
     4861 cgccctccta gttccgaaaa gcggacgagt cggggacatg acgcttctct cgctcccgtt
     4921 gtgtgctcgc tcgcgtcatg cccccgacca agattcggta tcgggcgacg catgcgtcac
     4981 tcctacgggg agacggggcg ggttgagaca acccgcccac ggtacgctac ggcttgagga
     5041 ggatcttcat ggaggcttcg gccttctgcg ccagctcgat cccctccaca cactgctcga
     5101 gcggcatcct ctgggtgatc atcgactcga tgtcgatgcg cctgtcggcc atcatctgca
     5161 gaccggtctc catgtcggcg tagttgaagg cccggcagcc gtgaaggtcc aactctttga
     5221 gggtgacgat cccgaaatcg aagggtcgcg gctccttcgg catcgccacg atgacgatct
     5281 gcccgcccac cttgcagaag tggcacatct gctcggtgag gggggccgct ccggcagcat
     5341 cgaacaggac gtcggcaccc cggccattgg tcctgtccat gatggcatcc ctgacgttca
     5401 cggtcttggc gtcgatggga tcgaagccaa gcttgcgggc gaactccagc cgccactcgc
     5461 cgatttccac gatgctcacc ggtcggttcg tggtgattcg agccatttga gcgaccagta
     5521 tcccaatggg gccgccgccc agcaccacga cgttatcccc gacgtgggca cgggacaccc
     5581 tgagggcgtg agcggacact gccgtcggct ccgccagcgc ggcgatatcc atcggcaagg
     5641 gatccggcat cttgtagatg tgcgagatgc gggtcttgac gtattcggca aaggagccgt
     5701 cggcgttgtg ccccagcatc ttgaggttga ggcaggagtt gtagatcccg tcgaggcagg
     5761 cctggcaaac ccggcaatcg agcagaggat ctgccaccac ccggtctccg accttcagtc
     5821 ccttcgcctc gcccttgatc tgcaccaact cgcccgagaa ctcgtgtccg atgatcactg
     5881 gagccactgc cctgggatgc ttaccgttga aaatggagac gtccgatccg cagatacccg
     5941 tataggcgac cttgatgagc gcctcgccct cattcagtgt cggtatcggg acatcctcca
     6001 atcggacgtc gtggcccttg tggtagacca ttgccttcat atgtcagatc ctctccttat
     6061 actccaagag agttggccgt gccaatccac gcagccctca ccccgtgaac atgtcctccg
     6121 gcatgttcac gcccctccct gagggagagg gggtagacgg ctcccaaccc ctaaccccca
     6181 tcccctatct cccgaccccc gatacccggc ccccgcttac gcgtgagtct gaccaaggca
     6241 ctgcaggccg ttgggcatga caatcatctc gggaacgtag gttcgggcgg gcaggctgga
     6301 caggaacagg caggcatcgg cgatgtcttg cggcgtcagt gccttgtcca gcgtctccct
     6361 ggaaggttga accgcccgct tcagcatgat gggcgtgtcg cacagcccgg gcatgatgat
     6421 gctgacccga acaccgttgg cccgctcctc cagcatggtc gcatgggcca gggcattcac
     6481 cccggccttt gccgcctggt aggcagcgcc ggagtagtcg gtgaaccgac cggacacgga
     6541 tgtgatgtgg ataagcaggc ccccgccctg cttgcgaaac tgaggaagga tcgcctgagt
     6601 taggtagtac gcgccgttca ggttggtgtc gacgagatgg tgccagttct cgtccgagag
     6661 aacggaaagc ttgcgatcgg gcgtgttggt gccgacggag ttgacgagga catcgatctt
     6721 gccaaaacag tcgactgctt cctggatcag ggcatcgacc tctgcgcgct tggaagcatc
     6781 ggccttgcgg aaccgcaact cgcaaccggc agccgcggct tccgctgcca actccttcag
     6841 tgactcctcc ttgcgcgcgg cggcaaagac ccggcaaccg gccttggcga gggtcagggc
     6901 cgtggctctc cccatgccgc tactggcgcc ggctacgatg gcagtttggc cggatagggt
     6961 acccatgagc atccacctcc atcagagttc gaatcacagg tctggcacgg gtcaaccgac
     7021 tggcgccacg cgacaacgcg cgacgttccg gcgaagcaca agtacgatcg acggttgggc
     7081 gaagcacgct tcgcccaacg gaaacgaact gcccaccgcc acgtggggcg gacgagcggc
     7141 cccgctccct gatcttgcgc aggctactac tccgccacgg tgctcccgac cggtgtcatc
     7201 ctgagcgaca gcgaaggatc tctcccttca cgaggagatg cttcgcttcg ctcagcatga
     7261 cagtacgtgt cacttctact gtggcggagc gctagggttt gagcaggatc ttcatggagg
     7321 catcgccctt gcgcgcgagc tcgagcccct caacgccctg ctccaacggc aacacgtggg
     7381 agatcatcga cttgacgtcg atcttgccct cggcgatcag attgatggca gcgcggtagt
     7441 cgtcggcgtt gtagacgcgg cagcctcgca ggtcgatctc cttcagagcg aaggcggcga
     7501 gatccactgg atggagttcc ttaggcatgg cgacgatcat cacctggccg tggaccttag
     7561 tgagcttggc cagttgagcg gcaacgggag cagcaccggc ggtgtcgaag agcacgtccg
     7621 cgcctttccc acccgttcgc cgcaacacct cctcgacggt gtccaccttc ttgggatcta
     7681 tgggatcgaa gcccagcctc ttggccagat c
//
LOCUS       DATALS010000381         4075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_42240,
            whole genome shotgun sequence.
ACCESSION   DATALS010000381 DATALS010000000
VERSION     DATALS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42240"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VHS06_08575"
     CDS             complement(<1..216)
                     /locus_tag="VHS06_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2988208.1"
                     /translation="MARDLHDSVTQSLYSITLFAEAAGERLADGNSSRAGQYLRELRN
                     TAQDALREMRLLIFELHPPVLEKEGLVG"
     gene            220..405
                     /locus_tag="VHS06_08580"
     CDS             220..405
                     /locus_tag="VHS06_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988209.1"
                     /translation="MPLLHHRRLPPLLFQPLVDGLGVKMAETGHQVSNTHVKLGEVAR
                     KLGQIDGGGGQKERQGG"
     gene            402..629
                     /locus_tag="VHS06_08585"
     CDS             402..629
                     /locus_tag="VHS06_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988210.1"
                     /translation="MTVHLRMEAASTRMEIWDDGIGFDPTAIHDRGGMGLLGMQERAH
                     RLGAELRIDSAPGIGTRLAVEMPAEGLEVSP"
     gene            626..1297
                     /locus_tag="VHS06_08590"
     CDS             626..1297
                     /locus_tag="VHS06_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2988211.1"
                     /translation="MSQGAIRVLVVDDHAIVRRGIGALLENKSDIHVVGEAADGQEAV
                     AQVEKLRPDVVLMDLVMPRMDGIEAIRQIAAHHPEARILVLTSFAADDKLFPALKAGA
                     LGYLLKDSAPEELVDAIRRVYHGESSLHPAVARKVLHELTHPSHHPAPGEALTDRELE
                     VLRLVARGLGTQEIAERLVITEATARSHVSNLLGKLQLSSRTQAVLYALREGLVSLED
                     TDQRE"
     gene            1495..1803
                     /locus_tag="VHS06_08595"
     CDS             1495..1803
                     /locus_tag="VHS06_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX2988212.1"
                     /translation="MIAVDVLLSGRLKLNGYGNGYQKNRDGTFHLALNEGAIVPDVIQ
                     RMGVPPAEVTMTMVNGHICRNGTALNPGDRVVLIPSDVAALWRHLGTMNMGQQGPLEF
                     "
     gene            complement(1848..2990)
                     /locus_tag="VHS06_08600"
     CDS             complement(1848..2990)
                     /locus_tag="VHS06_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2988213.1"
                     /translation="MTVHRLDTTLARDLKKLGAFDIDACFNCGNCTAVCSHSSETALF
                     PRKLIRYGQLGMRDRLASSREAWLCWNCRDCSDSCPRQARPSEYMESVRRYAVANLDP
                     TGISRLMYSSTLFVVGVALFLAALFAGVLLSRAGEPAAGILDLFGFIPFELIHDMGVV
                     VMAAIGLLAAINTVRLVGLLSRLIGPAGDGSEQSFANSGTPGRVLAAVRAVIEEMVVQ
                     RRFRDCEVDEEKPFYLRPWFVHYTIMWGFIGLGVATAIDLLFKTPGDVVPLWYPARLL
                     GTVAGLALMYGAALTIARKLRPLDRTQPRMGPSDWLFLGLLFLVGLTGFGLEAMVYVP
                     RGSALGYGIFLTHVVLAMELLVLFPFSKFAHALYRPLAYGIYRFRM"
     gene            complement(2987..3277)
                     /locus_tag="VHS06_08605"
     CDS             complement(2987..3277)
                     /locus_tag="VHS06_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2988214.1"
                     /translation="MSGKVASAEALKRLREQRAGALPELQARLKDQIKVTNEITRLLK
                     EGPRTVPALAEATGMPTQTVFWQLMALKKYGRVAEGEQDGDYFEYRLVGEER"
     gene            complement(3274..>4075)
                     /locus_tag="VHS06_08610"
     CDS             complement(3274..>4075)
                     /locus_tag="VHS06_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2988215.1"
                     /translation="CCTATLQAANELKELFPGTEVFDLYRDIRTYGRGHEEYYENASR
                     KAVLFFRYAPEESPMVERDESGSGYPVLVRVKDKLSWDEEIELPVDMVVLAVGMVPRP
                     ARELVEMLKLPVGSDGFLLEVHPKLRPVEVATNGILLAGTAQGPKDITESAAAASAAA
                     VKAATALTRGFVQLDPFVAVVDPAKCDGCGECAGACGYEGTIGFVEQAGVDGLRAEIN
                     GALCKGCGACVAACPKKAIELKGWTLEQFESMVDALVSDEVLAVEVAR"
BASE COUNT          760 a         1401 c         1244 g          670 t
ORIGIN      
        1 gcccaccagc ccctctttct cgagcaccgg tgggtgaagc tcgaagataa gcagcctcat
       61 ctcccgcagc gcgtcctggg cggtgtttcg cagctccctc aggtactgcc ctgctctgga
      121 gctgtttcca tcggccaacc gctcgccggc agcctcggcg aagagggtaa tgctgtagag
      181 ggattgggtc accgagtcgt ggaggtcgcg agccaaccgt tgccgctcct ccatcaccgc
      241 cgtctgccgc cgctgctctt ccagccgctg gtggacggcc tcggcgtaaa gatggccgaa
      301 acgggccacc aggtgtcgaa cacacacgtc aagctgggcg aggttgcccg gaagttgggg
      361 cagatcgacg gaggaggcgg gcagaaggaa cggcagggcg ggtgacggtc catctgcgga
      421 tggaggcggc ctctactcgc atggagatat gggacgacgg catcgggttc gatccgaccg
      481 ccatccacga caggggcggc atggggctgc tcgggatgca ggaacgggcg caccggctag
      541 gcgcggaact aaggatcgat agcgcgcccg gaatcggtac gaggctggcg gtggaaatgc
      601 cggcagaagg attggaggtg agtccatgag ccagggcgca atccgggtgc tggttgtgga
      661 cgaccacgcc atcgttcgta gaggcatcgg agccctgctg gagaacaagt ccgacatcca
      721 tgtggtgggg gaggctgccg acgggcagga ggctgtggct caggtggaga agctgcgacc
      781 ggacgtcgtg ttgatggatc tggtgatgcc gaggatggac ggtatagagg ccatccgaca
      841 gatcgcggcc caccaccccg aggcccgaat cctggtgctc accagcttcg ccgccgacga
      901 caagctgttc ccggccctca aagccggtgc tctgggctat ctcttgaaag actcagcacc
      961 ggaggagttg gttgacgcca tcaggcgggt gtaccacggt gagtcctccc tccaccccgc
     1021 agtcgcccgc aaggtgctcc atgagctgac ccatccctca caccatcccg ccccgggcga
     1081 ggccctgacg gatcgagagc tggaggttct gcggctcgtg gcccgggggt tgggcaccca
     1141 ggagatcgcc gaacggctgg tgataactga ggccacggct cgctcccacg tcagcaacct
     1201 cctgggcaag ctgcagctgt ccagccgcac ccaggcggtc ctctatgcct tgcgagaggg
     1261 actggtatcg ctggaggata ccgaccagag agagtaacgt cctctcgctc ctcatttacc
     1321 ctgccacgac tcaacgtccg tggaggccat ctccaccaac aacgatctcc caacacagac
     1381 catctccacg ttccgccgat ggagtcgaga gagcctcctg ctatcctgcg aatggttgag
     1441 aggctttgtg ccgctcttgg ccatcggctg ggttagttgg gaaagaggca agccatgata
     1501 gctgtcgatg ttctgctgtc tggaaggctg aagctgaacg gctacgggaa tgggtaccaa
     1561 aagaaccgcg acgggacctt ccacctggca ctgaatgagg gcgcgatcgt gcccgacgtg
     1621 atccagcgaa tgggcgtgcc gccggcggaa gtgaccatga ccatggtgaa cggccacatc
     1681 tgccggaacg gaacggcgct gaaccccggt gacagggtag tcctcattcc ctcagacgtc
     1741 gcggccctct ggcgacacct cgggaccatg aacatgggtc agcagggccc gctcgagttc
     1801 taggccgctg acaccggcca caagggctga gaaggctggg ccctgagtca cattctgaac
     1861 cggtagatcc cgtaggccag cggccggtag agggcgtggg cgaacttgct gaatgggaac
     1921 agcaccagca gctccatggc cagcaccacg tgagtcagga agatgccgta gcccagggcg
     1981 ctgcccctcg gaacgtacac catcgcctcc agcccgaagc cggtgaggcc caccagaaac
     2041 agcaacccca ggaacagcca atcggaggga cccatccgcg gctgcgtccg gtccagcggc
     2101 cgcagcttcc tggcgatggt gagggccgcc ccgtacatca acgccagccc tgccacggtc
     2161 cccagcaggc gggccggata ccacagcgga acgacatcgc ccggggtctt gaacagcagg
     2221 tctatggccg tggccacacc cagcccgatg aagccccaca tgatcgtgta gtggacgaac
     2281 caggggcgca ggtagaaggg cttctcctcg tccacctcac agtcccgaaa ccgcctctgc
     2341 acaaccatct cctcgatcac cgcgcgcacc gctgccagca ccctgccggg cgtgcctgag
     2401 ttcgcaaacg actgctcgga tccatcgccc gccggcccaa tcaatctaga caggagtccc
     2461 acaagcctca ccgtgttgat cgcggccagc agcccgatcg cggccataac caccacgccc
     2521 atgtcgtgga tcagctcgaa gggtatgaag ccgaacaggt ccaggattcc cgccgccggc
     2581 tcgccggccc ggctcaacag gacgccggca aagagggccg ccaggaagag ggcaaccccc
     2641 accacgaaga gggtggagct gtacatcagc cgcgatatcc cggttgggtc cagattggcg
     2701 acggcgtacc gcctcaccga ctccatgtac tcgctgggcc tcgcctgcct cgggcagcta
     2761 tcggagcagt cgcggcagtt ccagcagagc cacgcctctc tggaggacgc cagtcggtcc
     2821 cgcatcccca actggccata gcgaatcagc ttgcgtggaa agagggcggt ctccgagctg
     2881 tggctgcata cggccgtgca gttgccgcag ttgaagcagg cgtcgatgtc gaaggcgccg
     2941 agcttcttca gatcgcgcgc cagggtcgtg tccagtcgat gcactgtcat cgttcctcgc
     3001 ccaccagtcg atactcgaag tagtcgccat cctgctcgcc ctcggcgacc ctgccgtact
     3061 tcttcagcgc catcaactgc cagaagaccg tctgagtcgg catcccggtc gcctcggcca
     3121 gggcgggcac cgtcctgggc ccctccttca gcagtcgggt gatctcgttc gtcaccttta
     3181 tctggtcctt cagccgggcc tgcaactccg gcaacgcccc tgccctctgc tcccgcagcc
     3241 tcttcagcgc ctcggcggac gccaccttgc cgctcatcgc gccacctcca ccgccagcac
     3301 ctcgtccgaa accagcgcat ccaccatcga ttcgaactgc tccagggtcc accccttgag
     3361 ctctatagcc ttcttcgggc aggccgccac acaggccccg caccccttgc acagcgcacc
     3421 gttgatctca gcccgtagcc catcaacacc ggcctgctcg acgaagccga tggtcccctc
     3481 gtatccgcag gctccggcgc actcgccgca gccgtcgcac tttgccggat ccaccaccgc
     3541 cacaaaggga tccagctgca cgaagccccg cgtgagggcc gttgccgcct tcaccgccgc
     3601 tgcggaggcc gccgcggcac tctcggtgat atccttcggc ccctgggccg tgcccgccaa
     3661 cagtattccg ttggtggcca cctccacggg tcgcagcttg ggatggacct ccagcaggaa
     3721 gccatcggaa cccaccggga gcttcagcat ctccaccaac tccctggccg gccgcggcac
     3781 catgcccacg gccagcacca ccatgtccac cggaagctcg atctcctcgt cccaggagag
     3841 cttatccttc accctcacca gcacggggta gccgctcccg ctctcgtccc tctccaccat
     3901 gggagactcc tccggcgcat agcggaagaa gagcacggct ttgcggctgg cgttctcgta
     3961 gtactcctcg tggccgcgac cgtaggtccg tatgtcccga taaaggtcga agacctccgt
     4021 cccggggaag agctccttca actcgtttgc cgcctggagg gtcgccgtgc agcag
//
LOCUS       DATALS010000382         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_424925, whole genome shotgun sequence.
ACCESSION   DATALS010000382 DATALS010000000
VERSION     DATALS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424925"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VHS06_08615"
     CDS             complement(<1..219)
                     /locus_tag="VHS06_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988216.1"
                     /translation="MTRGVEWELADCLDRIERGDSTVEDVLIEHPELSAELEPLLGLA
                     VELGSLRQLSAPARMRGQGRPVFLDRPAE"
     gene            complement(221..799)
                     /locus_tag="VHS06_08620"
     CDS             complement(221..799)
                     /locus_tag="VHS06_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2988217.1"
                     /translation="MDVTIPPNVEEAGSTQDELQPLVQRASQGDGEAFGKLYDRYLDV
                     VYRYVYYKVGNSAEAEDLTSQLFMKAWEAMPRYQLREIPFSHWLMRLARNAVIDYYRT
                     RKPSGELDEAMVSREPDPQREYLRGEELRGLEVALRQLPEEQRSLLVMRFVEGMDYAE
                     VAAILGKNPGALRVMQHRALIALRRILDEEGK"
     gene            1221..>2710
                     /locus_tag="VHS06_08625"
     CDS             1221..>2710
                     /locus_tag="VHS06_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988218.1"
                     /translation="MYSVPAHRSLRCALPFSLPPGVLAVAMSLLLLIVASAAASAEMA
                     VEVTQIGWNGIAVPGAWNPVRVRATGDGANANVRIEVVSIARQQNGPQGATVVEYATG
                     AYGQEVALPAGVTKEVTLWIPVAAGGAPVGSAGLVRVTAGDRLLAEQRVEFRSTKTPF
                     WPLIGILAESPAPAKAISQVELPYQGLPIPLTVATLLPSDLPASGERLGAISALVVQG
                     SVAATLTSDQRQAVQDWVTAGGHLVLSGGPQAARVASILPQNALPVTFAATDAAIDLE
                     PLAKWAGVQENFPATGPATSFHAAIGSTLAGSTAQPLAWRMGMGQGTVTLLAVDPGLE
                     PLAAWSGTPSILRKALDPALPKPDENEKMRIIRAQEAEVPNRLQNVVDALPPGVFPSW
                     QNVALILGGFAILAGPLLHLLLWRADRRGWLWLSVPPAALLVAAGLYYFGIGRYGKDI
                     ITNTVAHVQMDPEGSQSKEWLLAGFYAPTHPRLTIDVPTDAPVRVL"
BASE COUNT          508 a          921 c          784 g          497 t
ORIGIN      
        1 ctcggccggc cgatcgagga aaaccggacg cccctggccc cgcatacgag cgggggcgga
       61 gagctgccgc aacgagccca actccacggc aaggcccagc aacggctcca actcagccga
      121 cagctccgga tgctcgatca ggacatcctc gaccgtcgag tcgccccgct cgatcctatc
      181 caggcaatcg gccagctccc actccacgcc cctggtcaac ctacttcccc tcttcatcca
      241 atatccgccg cagcgcgatc agtgccctgt gctgcatcac cctcaacgcg ccggggttct
      301 tccccaggat ggcggccacc tcggcgtagt ccatcccctc cacgaagcgc atcaccagca
      361 acgatcgctg ctcctccggc aactgccgaa gcgccacttc cagcccccgc aactcctccc
      421 ctcggaggta ctcccgctgg gggtcgggct cgcgggaaac catcgcttca tcgagttccc
      481 cactcggctt ccgcgtacgg tagtaatcga taaccgcatt gcgcgccagc cgcatcagcc
      541 aatgggagaa gggtatctcc cgcaactggt agcggggcat cgcctcccac gccttcataa
      601 agagctggga ggtcaagtcc tccgcttcgg ccgagttccc caccttgtag tagacgtagc
      661 ggtacacgac gtccaagtac cggtcgtaca gctttccgaa cgcctcgccg tcaccctgag
      721 aagcccgctg taccagcggc tgcagctcat cctgggtgga acccgcctct tctacattag
      781 gggggatagt cacatccata tcaacgtctg ccagggggag ttgttacagg ttggatggca
      841 catttctgga gattagtctg gcagtttaag ttgggagcgg gctcgcgtct atatgccatt
      901 agtactaatt cccgggagct gcatatgccc cgtggaagca ccacaactgc cgtgaaatgg
      961 taagggtcgg tcaaggccac ccttaccgga agatggcagg ctcagccaaa agcccgtggc
     1021 caagcctatc tgatctacta agatctcatg tagaatggca cagaatcaag gatgatgatg
     1081 aaactctttt gcacttcctg acgtcctaca cttgagtgcg ggttgaggca tggcctatca
     1141 ccacgctgtg ggcagtgtcc tgaccacgtt cctcttcagc caacatcgag gaatgccctc
     1201 gccaagagga gatcttgacc atgtacagcg ttcctgctca ccgttcactt cgctgtgccc
     1261 tccccttttc gctcccaccg ggcgtcctcg ccgtcgcgat gtcgctacta ctgttgatag
     1321 tcgcatcggc agcggcaagc gcggagatgg ctgtggaggt aactcagatc ggctggaacg
     1381 gcatcgccgt gcccggcgca tggaatccgg tgcgagtgcg tgccactgga gatggcgcca
     1441 acgccaacgt ccgaatcgaa gtggtctcca tagccagaca acagaacggc cctcagggtg
     1501 ctacagtcgt cgagtatgcg accggggcgt atgggcagga agtggcgctg ccggcagggg
     1561 tgaccaagga ggtcaccctc tggataccgg tcgcggccgg cggcgctcct gtcggctccg
     1621 ccgggttggt gcgggtcaca gcaggggaca ggcttctggc ggagcaacgt gtggagttcc
     1681 gaagcaccaa gacccccttt tggccgctaa tagggatcct cgctgaatct cctgccccgg
     1741 ccaaagccat ctcccaggtg gagctgccgt atcaggggtt gcccatcccc cttaccgtcg
     1801 ccaccctctt gccgtcggat ctacctgcat cgggcgagcg gttgggggcc atcagcgccc
     1861 tggtggtcca gggcagcgtc gcagcaaccc tcacaagcga ccaacggcaa gccgtccagg
     1921 attgggtgac agccgggggg catctggtgc tgtccggcgg cccgcaggca gcaagagttg
     1981 cctccatcct cccacaaaac gcccttccgg tgacatttgc tgccaccgac gccgcgatcg
     2041 acctggagcc gctggccaag tgggcaggag ttcaggagaa ctttccagcc acaggcccgg
     2101 caacctcttt ccacgcggcc atcgggtcta ccctggccgg ctcgaccgct caacccctgg
     2161 cctggcggat ggggatgggg caaggcaccg tgacactgct ggccgtcgat cccggactgg
     2221 agccgcttgc agcctggtcg ggcacaccct ccatcctgcg caaagcgcta gaccccgcgc
     2281 ttcccaaacc ggacgagaac gaaaagatgc gcatcatccg cgcacaggag gccgaagtcc
     2341 cgaaccggct tcagaacgtc gtggatgccc tgccgccggg ggtatttccc tcctggcaga
     2401 acgtcgcctt gatcctcggt ggcttcgcca tcctggccgg ccccctgctc cacctgctgt
     2461 tgtggcgagc cgaccgcagg ggatggctgt ggctgtccgt cccgcctgcc gcgctgctgg
     2521 tcgcggcagg tctctactac ttcggcatcg gacgctatgg taaagacatc ataaccaata
     2581 ccgttgccca cgttcagatg gacccggagg gcagtcaatc caaggagtgg cttctggcgg
     2641 gcttctacgc ccctactcac ccaaggctga ccatcgacgt ccccaccgac gcccccgtac
     2701 gcgtcctgag
//
LOCUS       DATALS010000383         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_266702, whole genome shotgun sequence.
ACCESSION   DATALS010000383 DATALS010000000
VERSION     DATALS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266702"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VHS06_08630"
     CDS             complement(<1..266)
                     /locus_tag="VHS06_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988219.1"
                     /translation="MKLRKRRFRQTCDTRCRSERDALAFVNELGFCGLIDSVDADIPN
                     LRQASVEPYEDPWGEVPARIWWDVKQTLPARKACYYAKVLRGRGT"
     gene            complement(294..716)
                     /locus_tag="VHS06_08635"
     CDS             complement(294..716)
                     /locus_tag="VHS06_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX2988220.1"
                     /translation="MLAGTSPHGSSYSPALWGPMWEAVPSPAVGLEFDPSHLVWQGVD
                     WMAALYEHGSRVVAFHAKDTEINQYKLEKDGNLMPGWWRYRIPGWGDVDWRQIFKALL
                     DLGYKGDMIIEHEDPVFSGDRREEGLKLGLAELHSHAL"
     gene            727..1320
                     /locus_tag="VHS06_08640"
     CDS             727..1320
                     /locus_tag="VHS06_08640"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX2988221.1"
                     /translation="MKQTLPARKACYYAKVLRGRGTFISWECFPYFYALYSTGNDYMD
                     DYRAGILSRDEKRILDLVAEQPSISSKELRRKFGPIGKDTSRAMDRAILPLQETFKIT
                     VSGGSIEGWSLHNWSLVEDWVPQEHLDKARTIAPQDALKELIVLSVRMSVAASIGDIA
                     WLFRRKRKEIAGLVHDLVNDGRLRDVEIEGLGEMFSV"
     gene            1313..2149
                     /locus_tag="VHS06_08645"
     CDS             1313..2149
                     /locus_tag="VHS06_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2988222.1"
                     /translation="MCEFCTKHGDGKKWYLQAKNYADDLASDLDRIEKTAERLKYFTD
                     GRFRRDYRLMERGYPRMPERLQRVFAARFTRQQKVSHFGQVLPIEDVEKILPMVNSIV
                     RLPCVCRKAVTGKEVAYCIGITTTPESTMGKLLCKSFDAGPDTAGVDRLTPEEALEFM
                     RGLERKGVMHSVWTFETPFIGAICNCERTDCVATRATVDHGLKTMFKGEYIAEVNPDL
                     CVGCGNCAELCQFDAIVMADRKACVDQSKCYGCGSCRAVCAKNAVTLLDRVAVPAVAN
                     DW"
BASE COUNT          438 a          657 c          672 g          396 t
ORIGIN      
        1 gtcccccgcc ctcgcagaac ctttgcgtag taacatgcct tccgcgccgg cagcgtctgc
       61 ttcacgtccc accatatcct cgccggcacc tctccccacg ggtcctcata cggctccacg
      121 gacgcctgcc gcaggttcgg gatatcggca tccaccgagt caatgagccc gcagaacccc
      181 aactcgttga cgaaggcaag cgcatcccgc tccgaccggc agcgcgtatc gcaggtctgg
      241 cggaagcgcc tcttcctaag cttcatcagt tcgtcgagag tcatagtcga aacctacaga
      301 gcgtgagagt gaagctctgc gagtccgagc ttcagtccct cttccctgcg gtcgccggag
      361 aagaccgggt cctcgtgctc gatgatcatg tcgcccttat agccgagatc cagcagcgcc
      421 ttgaagatct gacgccagtc aacgtcgccc cagccaggaa tccggtagcg ccaccagccc
      481 ggcatcaggt tgccgtcctt ctccagcttg tactgattga tctccgtgtc cttcgcatgg
      541 aaggcgacga cgcggctgcc gtgctcgtac agcgcagcca tccagtcaac gccctgccag
      601 accaggtgcg agggatcgaa ttccaggccg actgcaggcg acgggaccgc ttcccacatc
      661 gggccccaga gcgcggggct gtatgaggac ccgtggggag aggtgccggc gaggatatgg
      721 tgggacgtga agcagacgct gccggcgcgg aaggcatgtt actacgcaaa ggttctgcga
      781 gggcggggga cgttcatctc ctgggagtgc ttcccgtact tctatgcgct gtactcgacg
      841 gggaacgact acatggacga ctaccgcgcg ggcatactct cgcgtgacga gaaacgaatc
      901 ctggacctgg tcgctgagca gccaagcatc tccagcaagg aactccggcg caagttcggg
      961 cccatcggca aggataccag ccgggccatg gaccgggcga tccttccgct gcaggagaca
     1021 ttcaagatca cagtgtccgg cggctccatc gagggatggt ccctccacaa ctggtcgctg
     1081 gtcgaggact gggtgccaca agagcatctg gacaaggctc ggacgatcgc tccgcaggac
     1141 gcgctgaaag agctgatcgt cctcagcgtc cggatgtccg tcgccgcatc tatcggcgac
     1201 atcgcctggc tcttccgccg caagcgcaag gagatagccg ggctcgtcca tgatctggtg
     1261 aatgacggac ggctaaggga tgttgagatc gaaggactgg gggagatgtt cagtgtgtga
     1321 gttctgtaca aaacacggcg acggcaagaa gtggtatctg caggcgaaga actatgctga
     1381 cgacctggca tccgatctgg atcgtatcga gaagaccgcc gagcggctga agtacttcac
     1441 ggatggcagg ttccggcgcg actaccgact catggagcgt ggttaccccc ggatgccgga
     1501 gcgcctgcag cgtgtcttcg ccgcccgatt cacccgtcag cagaaagtat cgcacttcgg
     1561 tcaggtgctg cccatcgagg atgtggagaa gatcctcccg atggtcaatt ccatagtccg
     1621 cctgccttgc gtgtgccgaa aagccgtcac gggcaaggaa gtcgcctact gcatcgggat
     1681 cacgacgacg cccgagagca ccatgggcaa gctgctctgt aagtccttcg atgcaggacc
     1741 tgacacagcc ggcgttgatc ggctcactcc tgaggaggca ctcgagttca tgcgcgggct
     1801 ggagcggaaa ggcgtcatgc actcggtctg gaccttcgag acgccgttca tcggcgcgat
     1861 ctgcaactgc gagcggaccg actgcgtcgc cactcgcgcc acagtggacc atggactgaa
     1921 gaccatgttc aaaggcgagt acatcgctga ggtgaatccg gacctgtgcg ttggctgcgg
     1981 gaactgcgca gagctctgcc agttcgatgc gattgtcatg gcagacagga aggcatgcgt
     2041 ggaccagtcg aagtgctatg gatgtggctc ctgccgggcg gtatgcgcga agaacgccgt
     2101 cacactgctc gatcgcgtag cagtcccggc agttgccaat gattggtagg ggaactacat
     2161 aga
//
LOCUS       DATALS010000384         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_266845, whole genome shotgun sequence.
ACCESSION   DATALS010000384 DATALS010000000
VERSION     DATALS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266845"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VHS06_08650"
     CDS             <1..671
                     /locus_tag="VHS06_08650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX2988223.1"
                     /translation="PLLRRCLESLEHQSVTDWGLLVIDAGSDNGTTEFVEKVVSPRFP
                     GRVTLLQNLQPLTPIENIDTAIRRLCSNPESVIAMVDSDDALIGTDAASIVMGVYAGG
                     ADLTVGTMLRTDKHRRYPVDFVNPRASRGGNVWQHLRTFKKSLYDRVPSDYLKLDGEW
                     VRHTEDWAFMVPMVELSIKPVHISKSIYFYQPSPDKAERSVADRERIIAGILEKASLR
                     EAGT"
     gene            668..1237
                     /locus_tag="VHS06_08655"
     CDS             668..1237
                     /locus_tag="VHS06_08655"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX2988224.1"
                     /translation="MKSIIGVIGDVDFGGDALKREKAMSLGKALVSNGFRIAHGGLGD
                     IARTVSEGARSSPRYVDGDLIAILPGFDPADAADTADIVLATGLDMARNMIIANSDAV
                     VAVGGGAGTLSEMAFAWALKRPVLAFEVAGWSGALAGKKVDHRRRCDLLEDVVIGISN
                     AEQAVAVLNDVLTAYPKRHRGIQGGRANI"
     gene            1218..2054
                     /locus_tag="VHS06_08660"
     CDS             1218..2054
                     /locus_tag="VHS06_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2988225.1"
                     /translation="MEGPTSRPTGRSLARISFLGTALLGPQYRPAEDLIEIDVTYECN
                     LKCIGCNRSCRQAPDETRLSPAQLQKFIDETISTGRKWKRIRVMGGEPTTHPEIVQIL
                     WMLADYKTEYNPDVSLEVVTNGLWEGLHSLIRTFPSSVAVTNTHKASATSNKFENFNL
                     APKDDYHQAFTDFRNGCWIMEECGLGLTPYGYYPCAVAGGIDRVIGLNIGLKHLPSEA
                     QDGHTLTMQQRSLCQWCGHFRSREKLHTKDRPIASEPLYSLSWVKAYGSYSEQKPKLS
                     LY"
BASE COUNT          486 a          575 c          624 g          406 t
ORIGIN      
        1 tcccgctcct aagacggtgc ctcgagtccc tggagcacca atcggttaca gactgggggc
       61 tattggtaat cgacgccggc tcggacaacg gcacgactga gttcgtggag aaggtcgtca
      121 gtccccgctt ccccggcagg gtaaccctgc tgcagaacct ccagcccctg acacccattg
      181 agaacatcga caccgccatc aggcggctgt gcagcaaccc agagtctgtc atcgctatgg
      241 tcgactcgga cgacgccctc atcggcacgg acgcagctag catcgttatg ggggtctacg
      301 caggaggcgc ggacctcacg gtcggaacaa tgctgaggac cgacaagcat aggaggtacc
      361 cggtggactt cgtcaatccc cgggcgagcc gcggggggaa cgtctggcaa cacctgagga
      421 ccttcaagaa gagcctgtac gatcgggtac cctcagatta cctcaagctc gacggagaat
      481 gggttcggca cactgaggac tgggcattca tggtgcccat ggttgagttg tccataaaac
      541 cagtacacat cagcaaatcg atttacttct accagccgtc gccggacaag gcagagcggt
      601 ccgtggcgga tagggagcgc attatcgcag ggatactcga gaaggcatcc ctcagggagg
      661 caggaacatg aaaagcatca tcggggtgat tggtgacgtc gatttcggcg gcgatgcgct
      721 gaagcgggag aaagcaatgt cgttgggtaa ggctcttgtg agcaatggct tcaggatagc
      781 gcacggcggc ctcggggaca tcgccaggac cgtgtccgaa ggagccagaa gctcgccacg
      841 ctacgtggac ggtgatctaa tcgccatctt gccgggcttc gacccggctg acgctgcaga
      901 cacagcagat atcgtcctgg ccacaggtct cgatatggct aggaacatga tcattgccaa
      961 cagcgatgcg gtcgtggccg tcggcggcgg ggccgggacc ctcagcgaga tggctttcgc
     1021 ctgggctctg aagaggcctg tgctggcctt tgaggtcgcc ggctggagcg gcgccctggc
     1081 gggcaagaag gtcgatcacc ggaggaggtg cgacctactg gaggacgtgg tcattggcat
     1141 cagcaacgcg gagcaggcgg tcgcggtcct caacgacgtc ctgactgctt acccgaagcg
     1201 gcacaggggg atacaaggtg gaagggccaa catctaggcc tacgggtagg agcctggcga
     1261 gaatctcctt cctgggaacc gctctcctcg gacctcagta ccggccggcc gaggatctga
     1321 ttgagatcga cgtgacctac gagtgcaacc tgaagtgcat aggctgcaat agatcctgtc
     1381 gccaagcccc agacgaaacc aggctttctc ccgcccagtt acaaaagttt atcgacgaga
     1441 cgatcagcac ggggaggaag tggaagcgca ttagagttat gggcggagaa ccgaccacac
     1501 atcctgaaat cgtccagata ctctggatgc tcgctgatta caaaacagag tacaacccag
     1561 atgtctctct ggaggtagtc actaatggtc tctgggaagg gttgcattcg cttatacgta
     1621 catttccttc atctgtggcc gttaccaaca ctcacaaggc ctcggccaca tcaaacaagt
     1681 ttgagaactt caacctagca ccaaaggatg actaccacca agcttttacg gacttcagga
     1741 acggctgttg gatcatggaa gagtgtggcc ttggcctcac accgtacggt tactatccct
     1801 gtgcagtggc gggaggcatt gatcgagtga taggactcaa tattgggttg aagcacctac
     1861 cctccgaggc ccaagatggc cacactctga caatgcaaca acgaagcctg tgccagtggt
     1921 gtggacactt ccgttcccgt gaaaagttac acaccaagga tcgcccgatt gcttcggagc
     1981 ccctgtattc gttgtcttgg gtcaaggcgt acgggtcgta ctctgagcaa aagccaaagt
     2041 taagcctata ttgaacttgg agcaggccag ttattgatgt cattacctta a
//
LOCUS       DATALS010000385         3069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_64282,
            whole genome shotgun sequence.
ACCESSION   DATALS010000385 DATALS010000000
VERSION     DATALS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64282"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VHS06_08665"
     CDS             <1..1194
                     /locus_tag="VHS06_08665"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2988226.1"
                     /translation="GPLAGIRVIDLATMFAGPFAASLLADFGADVIKVELPKVGDANR
                     GMGPIVKGIPGPWTILARNKRSLSLDIRKDAGKEILKKLVATADIVVENFRPGTLEKW
                     GLGYDTLKTVNPKIILIRISGYGQTGPCCHKAGFGTPAAAFSGLTYLQGYPDRPPVSP
                     PLALVDYMTGTYGALAAVMALYHLKVCDCAEGQQADIALFESAFRMLEPVVAEYGLTG
                     KIKERGGHIGQGAAPSGTFKCKDGKWVVMVTSTQRTFERLAEVMGRTDLLKDAKFDTN
                     PHRAEHRYEIYDIVQEWFSHYTKAEVIRLLDENGVPVCPIQSIADIFEDPQYRARGDL
                     IEVDHPVLGKVTMPGIIPKFSDTPGAVRFPGPTKIGEHNHEILCDELGLTPEQMKALS
                     AEEVI"
     gene            1323..2261
                     /locus_tag="VHS06_08670"
     CDS             1323..2261
                     /locus_tag="VHS06_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX2988227.1"
                     /translation="MKLPSRVKIVEVGPRDGFQAERQWIPTEQKIEIVNALSRTGIKD
                     IQATSFVHPKAVPQLADAEEVMSKIDRPAGVEFRVLVPNMKGLERAIAYKPKRMNFMM
                     SVSESHNRSNANCSIEESLTKFEPMAAMALEAGIEPVGGMACTFGCPFEGEVPVAQLE
                     RVTRRYISMGIKEVGLSDTVGVANPMQVYNVAAHMLDKFPDVQWNLHLHNTRDMALAN
                     VVAAMQAGMTSFESSIGGLGGCPYAPAATGNVATEDLVNMLTEMGIETGVDLDALIAI
                     AKRAQEIIPHRLDSCMVRAGKRTDLKPAPKEQQKLG"
     gene            2563..>3069
                     /locus_tag="VHS06_08675"
     CDS             2563..>3069
                     /locus_tag="VHS06_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988228.1"
                     /translation="MANRSALKACISQLWVAFVAVLTMLAALVVLTACSSQSAAPTAA
                     PKAAEPTKAAAPAAAATTAPAAAPKADYPPKTVTFIAPSKPGSGFDTTARAIASTLEK
                     EKLITFALPVQNASSPPEGMATIVQQFKGKADMINVGSITTFLNKASGASPYGHKDLT
                     PIAALLSTN"
BASE COUNT          624 a         1039 c          924 g          482 t
ORIGIN      
        1 ggcccgctgg ccgggatcag ggtcatcgac ctggcaacca tgtttgcggg accctttgca
       61 gccagcctct tggccgactt tggcgcggat gtcatcaagg tcgaactccc caaggtgggc
      121 gacgccaacc gaggcatggg ccccatcgtc aaagggatcc ccggtccctg gaccatactg
      181 gccaggaaca agcgctccct cagcctggac atccgcaagg acgcaggcaa ggagatcctg
      241 aagaagctgg tagccacggc ggacatcgtc gtggagaact tcaggccggg cacgctggag
      301 aagtggggtc ttggctacga caccctcaag accgtcaacc ccaagatcat cctcatccgc
      361 atttcgggct acggccagac cggcccctgc tgtcacaaag ccggcttcgg caccccggcc
      421 gcggcattca gcggcctcac ctacctgcag ggctatcccg acaggccgcc ggtgagcccc
      481 ccactggcgc tggtcgacta catgaccggg acatacggtg ccctggccgc ggtgatggcg
      541 ctctaccacc tcaaagtctg cgactgcgcc gaggggcagc aggccgacat cgccctcttc
      601 gagtccgctt tcaggatgct ggagccggtc gtggccgagt acggcctcac cggcaagatc
      661 aaggagaggg gtggccatat cgggcagggg gcggccccgt ccggcacctt caagtgcaag
      721 gacggcaaat gggtggtgat ggtcaccagc acccaacgga ccttcgaacg gctggccgag
      781 gtgatggggc gcaccgatct cttgaaggac gccaagttcg acaccaatcc ccaccgcgcc
      841 gaacaccgct acgagatcta cgatatcgtt caggagtggt tctcccacta cactaaggcc
      901 gaggtgatca gactactcga cgagaacggt gtccccgtct gtcccatcca gagcatcgcg
      961 gacatcttcg aggatccaca gtatcgggcg cggggtgacc tgatagaggt cgatcacccg
     1021 gtgctgggca aggtcacgat gccggggatc atccccaagt tctcagacac cccgggcgcc
     1081 gtgcgcttcc ccggccccac caagatcggc gagcacaacc atgagatcct ttgcgacgaa
     1141 ctcggcctca ctccggaaca gatgaaagcc ctatccgcgg aagaggtgat ctgacgcagg
     1201 agcgcgcgat ccgtgggggg cgacgcatgg gtcgcccaac ttgcggcccc gtcccgtccg
     1261 agggcgacgc atgcgtcgcc cctgcagacc gggcggcggg cctcgcagac gaggagatcg
     1321 gaatgaagct acccagcaga gtgaagatcg tcgaggtggg tccaagggac ggcttccagg
     1381 cggagcgcca gtggatcccc acggaacaga agatcgagat cgtcaacgcg ctgtctcgta
     1441 ccgggatcaa ggatatccag gccacctcct tcgttcaccc caaggcggtg ccgcagctgg
     1501 cagacgccga ggaggtgatg tcgaagatcg atcggccggc cggtgtggag ttccgagtgc
     1561 tggtgcccaa catgaaaggg ctcgagcggg ccatcgccta caagccaaag cggatgaatt
     1621 tcatgatgtc ggtcagcgaa tcccacaacc gctccaacgc caactgctcc attgaggagt
     1681 cgctcacgaa gttcgagccc atggcggcga tggcgctgga agccgggatc gagccggtgg
     1741 gaggcatggc ctgcaccttc ggctgcccct tcgagggaga ggtgccggtg gcacaactgg
     1801 agagggtgac ccgacgctac atctccatgg ggattaaaga agtaggtctc tcggacacgg
     1861 tgggtgtggc aaaccctatg caagtctata atgtagccgc ccatatgctt gacaagttcc
     1921 ctgatgtaca gtggaacctg cacctccaca acacccgcga catggcgctg gccaacgtcg
     1981 tcgcagcaat gcaggcgggg atgaccagct tcgagtcctc tatcggcggc ctgggtggct
     2041 gcccctacgc ccccgctgcg acgggaaacg tggctaccga agacctggtg aacatgctca
     2101 ctgagatggg gattgagacg ggcgtggacc tggacgccct catcgccatc gccaagcggg
     2161 cacaggagat catcccgcac cgactggata gctgcatggt aagagccggc aagcgcaccg
     2221 acctgaagcc cgcgcccaag gagcaacaga agctgggatg accatctcga ctacgcagat
     2281 cccgcactag gagcccgggt tgccccaacc cgggcgggtg gcacgagagg ccgtcggcgc
     2341 aacgctgcgc tcgctaccgt tgcgggggca accccgactc ctaggccaca tccagcccac
     2401 cgaaaggagg tagacaacca cataggccag gtccttctgg cgtaggccag atccctctgg
     2461 cataggccaa gtcccccggg cagaatcctt gtccatggtc gagcttacgg aaccgtacct
     2521 gtcgagtagc tctttgttag tagctagtag gaggaggtaa ctgtggctaa ccgatctgcc
     2581 ctcaaggcct gcatctccca gttgtgggtt gccttcgtcg ccgtcctgac catgctggcg
     2641 gcgctagtcg tcctgaccgc ctgctccagc cagtcggccg cgccgaccgc tgcccccaag
     2701 gctgccgagc ccaccaaggc tgcagccccc gctgccgccg caaccaccgc tcccgcggca
     2761 gcccccaagg ccgactaccc acccaagacc gtaaccttca tcgcgccttc caagcccggc
     2821 agcggcttcg acaccaccgc ccgcgccatc gcctccaccc tcgagaagga gaagctcatc
     2881 accttcgccc tgcccgtcca gaacgccagc tctccccccg agggcatggc caccatcgtc
     2941 cagcagttca agggcaaggc cgacatgatc aacgtcggct ccatcaccac cttcctgaac
     3001 aaggccagcg gcgcctcgcc ctacggccac aaggatctca cccccatcgc cgccctcctt
     3061 tccaccaac
//
LOCUS       DATALS010000386         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_290134, whole genome shotgun sequence.
ACCESSION   DATALS010000386 DATALS010000000
VERSION     DATALS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290134"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VHS06_08680"
     CDS             complement(<1..1563)
                     /locus_tag="VHS06_08680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2988229.1"
                     /translation="MTLEVGLYLLALLVGMILRLLLIDARPLSVEEGALASESYRIWQ
                     GQSPESLHQGPMTAFGTALALALFAGGDGAARLVSALFGALLVGAPFLLRNSLGRAAA
                     LVATWGLAVSPLLLFASRDVGSGIVPLSLGLLLWVLVTGGTKAMTAIRGYALAVVLAA
                     LVACGAEGFSVLVTLGLAAILSHPRPRLLLSDLRVFLGTPLSRRMAFVFAAAALAIGT
                     GLGTNLRGVQWMLVDSWTAWLSGFSLSSPRSTLIWILLAYELPVVVLAIVQLLRTLPR
                     RERIDLFLSLWAMLLLLTCMLQTGGLAPRVVMPLLPIYLLASRLVGTSSSVAAQAGRG
                     WRWNYAVLAVIVPIAVGIILTNRGTTQGLEMPSVFLYGEAAALTASAVAIVLLLSGKE
                     RAALGWYTVVVLSVVFVVHNSVTTNFVTPSVGMEPVMGAQPTLALRSAARDASYYARF
                     YKTPVTVDTQLKSPLEWYLRDAQDVRYGTEQGEGISILLFRTPGPEPKSEAERRTGIV
                     TPAIDLANVTWQG"
     gene            complement(1793..>2358)
                     /locus_tag="VHS06_08685"
     CDS             complement(1793..>2358)
                     /locus_tag="VHS06_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988230.1"
                     /translation="WVATELRQALLRWASVICIALLVGVGLLYPLASTPSKAQDFRGN
                     PTLDGAVFYQSIKPDDYEAIRWLGTTAMGRPVVLEATGGDYTEYGRISTFSGLPSVLG
                     WGGHEVQWRGRGEEPQKRTVDIDSIYQTADRAAIRGLLDRYAVQYVFVGSLEVEKYGL
                     GVLNRFDGVLEPVHRQGKVTVYRVPGS"
BASE COUNT          481 a          773 c          705 g          399 t
ORIGIN      
        1 accctgccat gtgacgttgg cgagatcgat ggcaggcgtc actattccag tgcgccgttc
       61 tgcctccgac ttcggctcag ggcccggagt gcggaacaga aggatggata tcccttcgcc
      121 ctgttcggtc ccgtaccgaa cgtcctgtgc gtcgcgcaga taccactcca gcgggctttt
      181 caactgcgtg tctacagtga ccggcgtctt gtagaagcgg gcgtagtagc ttgcatctcg
      241 cgctgcgctg cgcagcgcaa gcgtcggctg cgcccccatc accggttcca tgcccaccga
      301 cggagtgacg aagttggtgg tgacgctgtt gtgcacaacg aatacgacgg agaggacgac
      361 tacagtgtac cacccgagcg ctgctcgctc tttaccactc agaagcagca ctattgcgac
      421 cgcggatgct gtcagcgcgg cagcctcacc gtagaggaat actgatggca tctccaatcc
      481 ctgggtagtt cccctattgg tgaggatgat gccaactgcg atcggcacga tcacggccaa
      541 cacggcgtag ttccatctcc atcctctgcc ggcttgtgct gccaccgacg aggaggtgcc
      601 gaccagccgc gaggccagca agtagatggg aaggagcggc atgacaaccc tgggggcaag
      661 tccgccggtc tggagcatgc aggtcaacag gagcagcata gcccacaggg agaggaagag
      721 atcgatccgc tccctcctcg gaagggttct cagtagctgt actatggcca gcactacgac
      781 gggaagttcg taggccagca gaatccagat cagcgtgctg cgaggtgatg agaggctgaa
      841 gccggacagc cacgcagtcc aggagtccac cagcatccac tgaaccccgc ggaggttcgt
      901 tccgagcccc gtgccgattg ccaaggcggc ggcggcgaag acgaatgcca tacgccgact
      961 gagtggagtc ccaaggaaga ctcgaaggtc ggacagcaac agccggggcc ttggatgaga
     1021 caggatcgcc gccagcccaa gcgtcacgag gaccgagaag ccctcagccc cgcacgcgac
     1081 gagggctgcc agaacgaccg ccagcgcata tcccctgatc gccgtcattg ccttcgttcc
     1141 gccagtcacc agcacccaga gaagaagccc caggctgagc ggcactattc cgctgcccac
     1201 gtccctggat gcgaacagga gtaacggaga gacggccagc ccccacgtgg caacgagggc
     1261 agcggcccgt cccagactgt tgcgcaggag gaacggagca ccgacgagaa gggcgccgaa
     1321 gagggcggag accagcctcg cagcgccatc gccacccgca aacagcgcaa gcgcgagcgc
     1381 agtaccgaag gctgtcatcg gtccctggtg gagagactcc ggagattgcc cctgccaaat
     1441 acggtacgac tccgatgcca gcgcaccctc ctcgacggag agtgggcggg catcgatcag
     1501 caacagccgt aggatcattc ccacgagcag cgcaagcagg tagaggccca cttccagtgt
     1561 caacacgccc tcgaggcggc tgacccatgc cctttgtggg gcctcctcgg cagctactgc
     1621 gtctcctttt tgtgcgacga ggcgaccttt cgtcgcaact gtcttcatac tcatcagtct
     1681 ggattgtccc taactgttcc gaatggatgg taggtgcgga cctctgtgtc cgccctccgc
     1741 accgggcgca cacgcaggtg cgcccctacc atcggccgat tcgccactgc ctctaactgc
     1801 cgggtaccct gtacaccgtg acctttccct gcctgtgcac gggctcaagc acaccgtcga
     1861 agcggttcag cacgccgagg ccgtacttct ctacctcgag gcttcccacg aacacgtact
     1921 gaacggcgta ccgatccagt aatcctcgaa ttgcggccct gtccgcagtc tggtagatgg
     1981 agtcaatatc cacggtccgc ttttgtggtt cctcaccccg ccctcgccac tgaacctcgt
     2041 ggccgcccca gccgagcacg gaaggcagcc cgctaaaggt ggatatgcgg ccgtactccg
     2101 tgtagtcacc ccccgtcgcc tcgagcacca cgggcctgcc catagcggtc gtgccgagcc
     2161 atcgaattgc ctcgtagtcg tcgggcttga tgctctggta gaagactgcg ccgtcgaggg
     2221 tgggattgcc ccggaaatcc tgggccttcg agggggtaga ggctaaagga tacaatagcc
     2281 ccacgcccac cagcaaggcg atgcagatga ccgacgccca ccgaaggaga gcctgtcgta
     2341 gctctgtcgc cacccatc
//
LOCUS       DATALS010000387         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_425307, whole genome shotgun sequence.
ACCESSION   DATALS010000387 DATALS010000000
VERSION     DATALS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425307"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VHS06_08690"
     CDS             <1..643
                     /locus_tag="VHS06_08690"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX2988231.1"
                     /translation="NFASADETLRAKSIQSLLSALKWADRLGATGVITHLGSSRDTEP
                     AQAMDFVCGCLELVLREAPKAPQLLVESCAGQGNTIGRRFDQLGEIVRRLGDDPRLQV
                     CVDTAHVFEAGYDIRTAEGLEATLREFDETVGLDRLKTLHVNDSKTPLGSNVDRHENI
                     GFGEIGEDAFARILRHPRLHPLPFLLEVPGMTKEGPDRPNINALRRLAGVDDD"
     gene            636..1391
                     /locus_tag="VHS06_08695"
     CDS             636..1391
                     /locus_tag="VHS06_08695"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX2988232.1"
                     /translation="MIDLRSALRGRPLVIAHRGASGEAPENSMAAFQLAIEQGADLIE
                     TDVHLTADGQLVAIHDHSLDRTTDGHGLVREHTLAQIKSLDSGAWFAPSFSGQKVLTL
                     DELLGWAANRIPVAIEIKHGPIYYEGIEERVVDTIRRHGMERSAMVISFDHAAVKRLK
                     RLSGEVLGGVLFAGTPMRASNLAIEAEAEAILPNWASITPEMVADAHDRGLAVSTWVT
                     DETAEMRWVLAHGVDAIATNRPGRLAALLQGQG"
     gene            complement(1420..>3799)
                     /locus_tag="VHS06_08700"
     CDS             complement(1420..>3799)
                     /locus_tag="VHS06_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988233.1"
                     /translation="FSVSALVGHIYQVVACYAIFRSMLVPFVHRPFVQLKAANVALEH
                     TVAELDARNRELDALDEVALSLSSTLKPDEILESAIERVMAVMRASAGAIFLREEETD
                     RMKLTAWRGLPQGILQESLSHIMYAPVPGLLAGDGDGSVDPPDNGSVIRRIGGSETRI
                     ASLGVCSCAPIRSKGRLMGVIAMVGDDGRKFGQRDSDLLTAIAYQLGLAIENARLYAH
                     TDERLREKVIELQRAERRSRFLFEVGALFGSGAEMTDVLGQVARRSAQVLGDWCSIYL
                     LDEREKLLRLAATHHADAHELVPMRELLQRRPVRLGEGVVGRVAERGEALLSTTVPVE
                     EVASEARVLAQSVEEIAILRSVAPHSRIAVPMRALGRTVGVLVVMATHSRKPLEQDDL
                     SLAIELADRAGAAIEAERLFQGIQAQRQHLEAIISQMVDGVVVTDVSGKVVVCNAAAQ
                     GMLDERIDQLLGTESSVMAGPSGRGGHAKGSTSLLVSRVLAGEVVLGESLTVSSTGRD
                     LVLSASASPVRTEDREIDGAVVVLRDVTAEREVERIKDEFVATVSHELRTPITAVLGY
                     TDILLRGLRGELSPKQTEAMSAVRNAGQRLLALINDLLDMSRLEAGKQSANLVPLDLS
                     SCIVRATATVSMLAGARGIAMESRVADGLPLVLADDELLQRILGNLLSNAIKFTPEGG
                     TVTISAAPVGGQKMAQFSFLSPGNEDGGAIAVLVTDTGVGIPLEHQERIWDKFQQVDS
                     SSRRMFGGTGLGLSITRGLVELHGGRVWVESEGVPGKGSTFGFTLQVASTDS"
BASE COUNT          772 a         1251 c         1101 g          675 t
ORIGIN      
        1 caacttcgcc tccgccgacg agacactgcg agcgaaatcc atccagtctc ttctttcggc
       61 gctaaaatgg gcagacaggc ttggagctac gggtgtgatc actcacctcg gaagcagccg
      121 cgacacggaa ccggcgcagg cgatggactt cgtctgcggc tgtctggaac tagtgctgcg
      181 agaggcgccg aaggcgcccc agctactcgt cgagagctgt gcggggcagg gcaacaccat
      241 cggacggcga ttcgaccagt tgggggagat cgttcgacgg ctcggggatg atccaagact
      301 acaggtatgt gtggacactg cccacgtatt cgaggcaggc tacgacatcc gcacagcaga
      361 ggggctcgag gcaacactgc gcgagttcga cgagaccgtg ggcctggaca ggctaaagac
      421 gcttcacgtc aacgactcca agacaccact gggttccaac gtggatcgcc acgagaacat
      481 cggattcggt gagatcgggg aggatgcctt tgccaggatc ctccggcacc cacggctaca
      541 tcccctcccc ttcctcctgg aggtccccgg catgacgaaa gagggtccgg accgacccaa
      601 catcaacgcg ctgcgaaggc ttgcgggggt agacgatgat tgacctgaga tcagccctgc
      661 gggggcgtcc tctcgtgatc gcccacagag gtgcttcagg agaggcgccc gaaaacagta
      721 tggcggcctt ccagcttgcc atcgaacagg gcgcggatct gatcgagacc gatgtccacc
      781 tgacggccga tggccaactg gtggccattc acgaccattc gctcgatcgc acgaccgatg
      841 gccacgggct ggtacgggag catacgcttg cgcaaatcaa gtcgctggac tcaggggcat
      901 ggtttgcgcc aagcttttcg ggtcagaagg tgctgaccct ggatgaactg ctcggctggg
      961 cggcgaacag gattcccgtg gccatcgaaa tcaaacacgg ccccatctac tacgagggca
     1021 tcgaggagag ggttgtggac accatcaggc gtcacggcat ggagcgctct gccatggtga
     1081 tctccttcga tcatgcagcc gtgaagcggc taaagcgatt atccggagaa gtattgggtg
     1141 gggtgctgtt tgcgggtacg cctatgcgcg cctccaacct cgcgatcgaa gcggaggccg
     1201 aagcgatact gcctaactgg gcaagcatca cccctgagat ggtggccgac gcccacgatc
     1261 gagggctcgc cgtgagcacg tgggtgacgg acgagaccgc agagatgcgg tgggtcttgg
     1321 cccatggcgt cgatgcaatc gcgaccaatc gcccgggcag actggctgcc ctgctgcagg
     1381 gccaagggta atgttgtagg ggcgacgcat gcgtcgcccc tacgaatcgg ttgaagcaac
     1441 ctgaagcgta aacccaaagg tgctgccctt acccggcacc ccctcgctct ctacccacac
     1501 cctaccaccg tgcaactcca ccagccctct ggtaatggac agtcccaatc ccgtaccgcc
     1561 gaacatcctg cgcgacgatg agtccacctg ctggaacttg tcccaaattc gctcctggtg
     1621 ctctagggga atccccaccc cggtgtctgt caccaatacg gcgatggcac ccccgtcctc
     1681 atttccgggc gacaggaagg agaactgcgc catcttctgt ccgccgaccg gcgccgcaga
     1741 gatagtcacc gttccccctt ccggcgtgaa cttgatggcg ttggagagta ggttgcccag
     1801 tattctctgc agaagctcgt cgtccgctaa gacgagagga agtccatctg ctacgcggct
     1861 ctccatggca atccctctgg caccggcaag catagaaacc gtcgctgtcg cacgcacgat
     1921 gcaggacgaa agatccagag gcaccaggtt ggcagactgc ttgccggcct ccaggcgcga
     1981 catgtccagc agatcgttga tgagcgcgag taaccgctgc cctgcattcc tgaccgcgga
     2041 catggcctcg gtctgcttgg ggctcaactc cccgcgcagc cctcggagca ggatgtctgt
     2101 gtagcccagg actgctgtga tgggagtccg aagctcgtgg gagacggtgg caacaaactc
     2161 atccttgatt ctctccacct cgcgctctgc cgtcacgtcc ctgagaacaa ccaccgcgcc
     2221 gtcgatctct ctatcctcgg ttcgcaccgg gctggcgctg gcgctgagca ccaggtcacg
     2281 tcctgtgctg gagacagtca gagactcacc caggaccacc tcccctgcca gcaccctgga
     2341 caccagcaaa gaggtggatc ccttagcgtg cccacccctt ccactaggtc cggccatgac
     2401 actcgactcg gtgccaagta gctggtctat ccgctcgtcc agcatgccct gtgctgcggc
     2461 gttgcacacc accacctttc ccgagacatc ggtcaccaca accccgtcca ccatctgaga
     2521 gattatcgct tcgaggtgct gtcgctgagc ctgaatgccc tggaagagcc gctcagcttc
     2581 gatggcggcc ccggccctat ccgcgagctc gatcgccaga gagaggtcgt cctgctccag
     2641 cggcttacgt gaatgggtcg ccatgaccac caggaccccc acagtcctgc ccaacgcccg
     2701 catcggaacg gctatacgag aatggggagc gacactgcga aggatcgcta tctcctccac
     2761 agactgagcg aggactcgag cctccgaggc gacctcctcc accggaaccg tggtcgacag
     2821 caacgcctcc ccacgctcgg caacacgccc aacgacccct tcacctagtc tcaccgggcg
     2881 gcgctggagc aattcgcgca tggggaccaa ctcatgagcg tctgcgtggt gggtcgccgc
     2941 cagccgcagc aacttctcgc gctcgtccag gaggtagatg gagcaccagt ctcccagcac
     3001 ttgcgcactc ctgcgggcaa cctgtcccaa cacatccgtc atctcggcac cgctcccaaa
     3061 gagcgcccct acctcgaaca ggaagcgtga cctgcgttcc gcccgctgca gctcgatcac
     3121 cttctcccga aggcgctcgt ctgtatgagc gtacagtcgg gcattttcga tggccaggcc
     3181 cagttgatag gcgatagcgg tcagaaggtc ggagtcccgc tgtccaaact tcctgccatc
     3241 gtcaccaacc atggcgataa cgcccatcag tctcccttta ctgcgaatgg gggcacatga
     3301 acagacgccg agggacgcga tacgagtctc cgagccaccg atccgtcgaa tgacactgcc
     3361 attgtcggga ggatctacgc tgccatcgcc atcccccgcc aacagtcccg gcactggagc
     3421 gtacatgatg tgactcagac tctcctgcag gatgccctga ggtagtccgc gccaggccgt
     3481 cagcttcatc ctatcggtct cctcttctcg caagaagatg gcaccggcag acgccctcat
     3541 gacggccatc accctttcaa tagcggactc caggatctcg tccggcttga gggtgctgct
     3601 gagagacaag gccacctcat ccagggcgtc cagttcccgg ttcctggcgt ccaactccgc
     3661 aactgtgtgc tccagcgcca cgttggctgc cttcagctgg acaaacggcc gatgaacaaa
     3721 cgggaccagc atggagcgga agatggcata gcaggcgacc acctggtaga tgtgccccac
     3781 gagcgcagac acgctgaag
//
LOCUS       DATALS010000388         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_43146,
            whole genome shotgun sequence.
ACCESSION   DATALS010000388 DATALS010000000
VERSION     DATALS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43146"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1503
                     /locus_tag="VHS06_08705"
     CDS             <1..>1503
                     /locus_tag="VHS06_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX2988234.1"
                     /translation="DAIFLVQVPSGRLVDVNESACHQLGCAREVLLDKLVYEVVDLSA
                     SPPMADLLTGGKGGTTRETINTMLRRCDGSGLLVEITVRLVTFGEEAYAVAVARDITE
                     RERLASEIRSQRNLLRAVFDNVLAGIVVLRGPDLVYEMVNAAYQAIAPEREFLGKSIA
                     EAWPEIADQVKPIGEMVFRTQQPYHVVDMPLKIRRSPDAPLEEAFFTSTWVPMPPARN
                     GDQSIVVSALETTEQVKARRAVESRAAELDSVISSIADGVIISDSNGQIVRMNAAARE
                     MVGLSEEKWHLPVEKQLELLRIETPDGKPLLPENVPIVRALRGERVEGVVIVVHPPDR
                     RSVWITASAAPVRVTEGRLLGAVSIFADITPIHDLEEQRSKYILGISHGLRTPLTVVQ
                     GQAQLLLQAIEKTDLNGRLRRGTEAIVGSAHRMSVMLRDLVDLMNLETGQPLRLNRVP
                     LNLSSFVLELKERLRGLLEVDRVRVRMPEELPPILADPDRLERILVNLLS"
BASE COUNT          289 a          422 c          500 g          292 t
ORIGIN      
        1 cgatgccatc tttctggtgc aggtcccctc cggaagattg gtcgacgtca acgagtcggc
       61 ttgccatcag cttggctgcg ctcgcgaggt gttgctcgat aagttggtct acgaggttgt
      121 cgatctgtct gcatcaccgc ccatggccga ccttctgact gggggcaaag gcggcacgac
      181 gcgagaaacg atcaacacga tgctccgtag atgtgatggc agcgggttgt tggtcgagat
      241 tactgtgcgc ctggtgacct tcggcgagga ggcctatgcc gttgcagtgg cccgcgacat
      301 caccgagcgc gagcgcctcg cgtcggagat ccgatcccag cggaacctgc tgcgagcggt
      361 gttcgacaac gttctggccg gcatagtcgt cttgaggggg ccggatctcg tgtacgagat
      421 ggtgaatgcg gcctaccagg ccatagcacc cgagcgggag ttcctcggca agagcatcgc
      481 ggaggcgtgg cctgagatag ctgatcaggt gaagccgatc ggagagatgg tttttcgcac
      541 gcagcagccc taccacgtcg tcgatatgcc tctcaagatt cgccggtcac ccgatgctcc
      601 cctcgaagag gctttcttca cctcgacctg ggtcccaatg ccaccggcaa gaaacggcga
      661 ccaatccatc gtggtgtcgg cattggagac caccgagcag gtgaaggctc ggagggctgt
      721 cgagagcagg gccgcagagc tggattcggt gatctcgtcc atagccgacg gggtaatcat
      781 ctccgactcg aatggacaga tcgtacgcat gaatgcagct gcacgggaga tggtcgggct
      841 ctcggaggag aagtggcact tgcccgtcga gaagcagttg gaactacttc gtattgagac
      901 tccagatggc aagcccctcc tgccggagaa tgtaccgata gtacgggcac tgcggggcga
      961 gagagtggag ggtgtcgtga tagtcgtcca tcctcccgat cggcggagtg tgtggattac
     1021 tgcgagcgcg gcgcccgttc gcgtcaccga aggtcgcttg ctgggggcgg tttccatttt
     1081 tgccgacatc accccaatac acgacctgga ggagcagcgg agcaagtaca tcctgggcat
     1141 ctcccatggg ctgcgaaccc cattgacggt ggtccagggg caggctcaac tgctgctcca
     1201 ggcgatcgag aagaccgacc tgaacggccg gttgcggcgc ggcacggagg caatcgtcgg
     1261 gtcggctcac aggatgagcg tgatgctgcg agacctggtg gacctgatga acctggaaac
     1321 cggacaaccg ttgagactga accgggtgcc gttgaacctt tcatcctttg tgctggagtt
     1381 gaaggagcgg ctgcgcgggc tcctggaggt cgaccgggtt cgggtgcgga tgccggaaga
     1441 gttgcctccg atcctggccg accccgatcg actggagcgg atcctcgtca acctgctcag
     1501 caa
//
LOCUS       DATALS010000389         9633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_399148, whole genome shotgun sequence.
ACCESSION   DATALS010000389 DATALS010000000
VERSION     DATALS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399148"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VHS06_08710"
     CDS             <1..888
                     /locus_tag="VHS06_08710"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX2988235.1"
                     /translation="ITRMDARVIVYEPDGTFVTSWGEGVFTPRTHCIKFGPDGSVYAA
                     DDGNHTVRKFTPDGKLLMTLGTPGVASDTGYNPKAGSSYDKLASITHGGDPFNRPTGV
                     AIAANGDIYVTDGYGNARVHRFSSDGKLIQSWGEPGTDPGQFNLPHDIWVMPDGRIMV
                     ADRENDRIQFFSPDGKFLDQWTHVQRPTGLAMDKDGLIYVSELWRRVGQTSFRNGPTT
                     EDKQGRVSVLDQTGNVLARFGNTGDRMAPGNFIAPHGIGVDSKGDIYVSEVTETFGVK
                     AAGLPSGGHCFQKFTRVRA"
     gene            1078..2052
                     /locus_tag="VHS06_08715"
     CDS             1078..2052
                     /locus_tag="VHS06_08715"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX2988236.1"
                     /translation="MRKTHLWFGIGLVAVLLLTLALSGCRASEAKYPSRPVTMAVGAS
                     AGSPQDLQGRVLAKASEKLLKSSISVENRAGGGGAEVFNYVKDQPADGYAVGMTTRSS
                     VYILARPDLPAKSEDFEWIIRLTSDPTAIIVRPDSQFKTIEDLIQYAKANPGKIKIGG
                     ALTGGINQAMMELFSTKAGIKMTWVAFDGASQATTAALGGQVDAIQMTPGSAVALLTE
                     GKLKALGVTSDKRLSYMPDSPTFKERGIDMVEYNWRGAFVKKGTPPEIVKTLHDTFKQ
                     AMDAPEWKDYVTKNKQEETYMNTADFNKAIVTETESAKAFLKQIGTIK"
     gene            2076..2516
                     /locus_tag="VHS06_08720"
     CDS             2076..2516
                     /locus_tag="VHS06_08720"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX2988237.1"
                     /translation="MKLDRIAAAVCFLVGAVIVREGTQLTYMSGFSVGPGFFPFWLGL
                     ALMLLSAILFITTRAPNQPFIESASGFRKAAMVAVAVLVYVVGLSYLGLLLALAILLA
                     FLVGVVERKSWRVWAPVALLGSLGCYLIFEVWLSVPLPIGVLGI"
     gene            2582..4087
                     /locus_tag="VHS06_08725"
     CDS             2582..4087
                     /locus_tag="VHS06_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX2988238.1"
                     /translation="MEVIQNLAHGLMVAMYPSNLVACFIGVLIGTLVGILPGLGPAAT
                     LAMLLPMTFGMGATTSMIMLAGVFYGSKYGGAITSVLVNLPGESSSLMTCMDGYPMAR
                     KGRAGPALGMSAIASFFAGTVGVIGLMLVAPPLADLALNFGPPEYFALMCLGLTLVTF
                     LAGKSMVKALIMGCVGLILSTVGSDTMSGSYRLWFGQPFLLGGVDFVVAAMGLFAVGE
                     ILMTIEEEAAVETIPSPKKLSELLPSFHDIRECWWTLVRSTLLGFLIGTVPGGNPTIA
                     TFLSYGATKAIAKDPEVFGSGTIRGVAASESADNAATSGGMVPLLTLGVPGTASTAIL
                     LGALIMAGLQPGPLLMQQEPELFWATIASMYIGNVLLLIMNLPLVPVFASLIRVPYFV
                     LYPMILAICSVGVYSLDNKVSDVGLMAVFGIIGYLMKKAEFPAAPLVLALVLGGMIEK
                     SLRQSLTLFEGDLTVFFQRPLCAVLLTLTVVMLLLPVVRGTRKRPAPVPQQ"
     gene            4131..5033
                     /locus_tag="VHS06_08730"
     CDS             4131..5033
                     /locus_tag="VHS06_08730"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX2988239.1"
                     /translation="MVETGTVRALLQGSYDLHVHSAPDIMKRKGSDLVMARRAAAAGI
                     QGMLFKSHNSSTADRVAVVNETAPGCRAYGALTLCHAVGGLNAAAVDVAARLGARLVW
                     FPTMDAANEVAYQDAHPNGKKAYWFVAQQELRQQRVSRPPISIIDGDGALVPAVHEIL
                     EVIAAHDMVLATGHLGVTEIVRLVAAAKAARVQRIIVSHPEYPSVAMPIVLQVELARQ
                     GMLMERCYRTPYSGKIGWEEQLAAIRAVGPSASIVCSDMGQVDAPWPEEALFDFVQRL
                     LEAGFTTEEVRVMTADNPRSVIEL"
     gene            5088..5924
                     /locus_tag="VHS06_08735"
     CDS             5088..5924
                     /locus_tag="VHS06_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2988240.1"
                     /translation="MPYADIGGYSMYYELDNFTDPWKQPETVLLHAGYCRNSRFWYAW
                     VPGLARHYQVLRPDHRGSGESPSPGPDFQPTIEGFVDDVMALMDHLRLERVHYVGESF
                     GGVVGIQMALTHPERLHSLTLCNTGFRILPKALTANSAGFPDRLDAIDELGLEGWCRK
                     TIDVRLDQERASAELSNWYCQEIGRADPLLAKRLSMVTRYVDFSPRMAEIKVPTLLLA
                     PGKSKITDLEQQRFMANAIPDAKLVVIENVGHGVNVLEPERCTLELLDFLAGRKAGAS
                     QA"
     gene            6077..7231
                     /locus_tag="VHS06_08740"
     CDS             6077..7231
                     /locus_tag="VHS06_08740"
                     /inference="COORDINATES: protein motif:HMM:NF016210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HEX2988241.1"
                     /translation="MATRQVKCSIIRGGTSKGVFVERDELPPHGPERDKIILALYGSP
                     DKRQIDGLGGADKLTSKTAIMGAPTRSDCDIDYHYGQVGTDLPTIDWTSNCGNISAGA
                     AAYAVYKGYVRATGPSALVRIHQVNTGRRLLATVPMADGMPATEGDFKIGGVPGTGAR
                     IDLDFSDFTGCILNRGLLPTGSPMDRFKVPGIGMLDVSVVDVANLCAFVRGSDVGMDN
                     TEDIESLQANQEIVAKLEAIRAVVALETGLVSRDVDTDRELRRRVNPLMFIVGTPRDY
                     DALNGEHIDAGNTDIFARSLARWAFSKTYPATGSIGTGVACTVAGSIPAEVVRGGPPA
                     RGETRSVRIGHPSGTLVTEAGISADGQLVIRGSVGRTARVLMEGTAFVRV"
     gene            complement(7302..8300)
                     /gene="pdxA"
                     /locus_tag="VHS06_08745"
     CDS             complement(7302..8300)
                     /gene="pdxA"
                     /locus_tag="VHS06_08745"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX2988242.1"
                     /translation="MSDSRPILGVTMGDPSGSGPEILVKALTTPEVRAASRPIAIGDG
                     ATIERAASIVGSTVQVRSIERVADATFQDGVLEVLDLHNVDLSQLQYGKVQAASGEAA
                     FQSIVKGVELAKAGETAAIVTSAINKESLNLAGHHYDGHTEILADLCGIKSVCMLLVA
                     GNFRVTHVSTHCSLRQAIERAKKPRIMDVIRMTHQATKMLGVAEPRIAVSGLNPHSGE
                     GGLFGDEEIKEITPAIEAMKAEGFNVVGPIPPDTVFFRMHEGQYDAVVAMFHDQGHIP
                     TKVIAFDEGVNVTLGLPIIRTSVDHGTVFGKAGKGTARPTSMLAALRLAAQMAKNR"
     gene            complement(8297..>9633)
                     /locus_tag="VHS06_08750"
     CDS             complement(8297..>9633)
                     /locus_tag="VHS06_08750"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEX2988243.1"
                     /translation="SDRGPGARGWGSGKASTALLDSISLVPDTRPPGPALHPWANVMT
                     QLGIVADDLTGAMDTGVQSAKAGLRAVVMLGNGELPPAEMVVISTDSRDEPAEVAYRH
                     AREAAGRLAGRLVYKKMDSTLRGNLGAEIDGMLDGLRLERALVAPAFPNTGRTTEGGY
                     HKVHGVLLSESAFARDPVWPATESHLPTIIARQTRRAVGHLSLEVVEQGPEEVIEALK
                     REKAPIVAADAVESRHLRTLAEALVRMDRLWLPAGCAGLAEEWIAALGATRSSEGGIG
                     WSADSRPVLVVAGSRNRTTAAQLQEASRRGLAEIMDLPSDADWGELVGVKSAELLGRR
                     HNVAITTTFAPYQEGQGMANAQRLADATARLLEMHEVSGVFMTGGDIARAVCLRLGAT
                     GLRALGEVQPGVPAGVLVGGRSDGLRVVTKAGGFGDEKAIIAAIHCLQGRLS"
BASE COUNT         1726 a         2939 c         3112 g         1856 t
ORIGIN      
        1 atcacccgca tggacgcccg cgtcatcgtc tacgagcccg acggcacctt cgtcacctcc
       61 tggggtgagg gcgtcttcac cccccgtacc cactgcatca agttcggccc cgacggctcc
      121 gtctacgctg ccgacgacgg caaccacacc gttagaaagt tcactcccga cggcaagctc
      181 ctcatgaccc tcggcacccc gggagttgct tccgatacag gctacaaccc gaaggcgggc
      241 tccagctacg acaagctggc gagcatcacc cacggaggcg atcccttcaa taggcccacc
      301 ggcgtcgcca tcgccgccaa cggcgacatc tacgtgaccg acggctacgg caacgcccgg
      361 gtgcaccgct tctcctccga cggcaagctg atccagtcgt ggggagagcc aggaacggat
      421 ccgggccagt tcaacctccc gcacgacatc tgggtcatgc cggacggtcg catcatggtg
      481 gccgatcgcg agaacgatcg gatccagttc ttcagccccg acggcaagtt cttggaccag
      541 tggacccacg tccagcgtcc gaccggcctg gccatggaca aggacgggct gatttacgtg
      601 tccgagctgt ggaggagagt cgggcagacg tccttccgta acggacccac cactgaggac
      661 aagcagggca gggtgagcgt gctggaccag acggggaacg tgctggcccg cttcggcaac
      721 acgggggacc ggatggctcc cggcaacttc atcgcccccc acgggatcgg ggtcgactcc
      781 aagggcgaca tctacgtctc cgaggtcacc gagaccttcg gcgtcaaggc cgcgggcttg
      841 ccctcgggcg gtcactgctt ccagaagttc acccgggtcc gggcctaggc gggaacgcct
      901 gccggccgga gaagcgcagg cacgttcggt ggtggttggg cggaccgagt aggacttcgc
      961 ctggggaagg ctcgctgagg caagcggggg tatcttgcct tgccgagcca agaggtaggt
     1021 ccgcctttgg gcgaccgaga acaacgaggg cacaaagcga gagagaaagg gaacaccatg
     1081 cggaagacac acctgtggtt tggcatcgga cttgtggcgg tgttgctact gacattggca
     1141 ctctcaggtt gcagggcatc tgaagccaag tatccgtcga ggccggtcac aatggcggtt
     1201 ggcgcctctg ccggatctcc tcaggacctt cagggaaggg tgttggccaa agcgtcagag
     1261 aaactcctga agtcctccat ctcagtcgag aacagagccg gtggcggagg ggccgaggta
     1321 ttcaactacg tgaaggatca accggccgac gggtacgcgg tcggcatgac taccaggagc
     1381 tctgtctaca tcctggccag gccggacctt cccgccaaat ccgaggactt tgaatggatc
     1441 attcgactga cgtcggaccc gacggcgatc atcgtgcggc cggacagcca gttcaagaca
     1501 attgaggacc tgatccagta cgcaaaggcg aaccccggca agatcaagat tggcggcgcc
     1561 ctcacaggcg gtatcaacca ggccatgatg gaactcttct cgaccaaggc cggcatcaag
     1621 atgacgtggg tggccttcga cggcgcttct caggctacga cagccgcgct gggcggacag
     1681 gttgatgcca tccagatgac gccgggttct gctgtggcgc tgcttaccga agggaagctc
     1741 aaggcgcttg gcgtcaccag cgacaagcgg ctctcctata tgcccgatag cccgactttc
     1801 aaggagcggg gcatcgacat ggtcgagtac aactggagag gcgctttcgt gaagaaggga
     1861 acccctcccg aaatcgtgaa gaccctgcac gacaccttca agcaggcgat ggacgctccg
     1921 gagtggaagg actacgtgac gaagaacaag caggaagaga cctacatgaa cactgctgac
     1981 ttcaacaagg ctatagtcac cgagactgag tcggctaagg ccttcctgaa gcaaatcggc
     2041 accatcaagt aaccaggtga gagggggctc tgggagtgaa actggatcga attgccgctg
     2101 cggtctgctt cttggttgga gcggtgatcg tgcgcgaggg cacgcagttg acctacatgt
     2161 ccggctttag cgtggggccg ggtttctttc ccttctggct cggacttgct ctgatgctcc
     2221 tgtcggcgat cttgttcatc actaccaggg ctccgaacca accattcatt gagagtgcat
     2281 caggttttcg caaggcggcc atggtggcgg tggcggttct tgtgtacgtc gtcggcctta
     2341 gctatctggg gctcctcctc gcactcgcga ttctcctggc cttcctggtg ggagtagtcg
     2401 aacgaaagag ctggcgagtg tgggccccgg tagctttgct gggcagcctc ggctgctacc
     2461 tgatattcga agtctggttg agtgttcccc tacctatagg ggtattaggt atctgagtgg
     2521 ggatattagg tatctgagcc aagaataatg gcaacgagct ctgcctcgtc tggagatgag
     2581 aatggaagta attcagaacc tggcccacgg cctgatggtt gccatgtacc catccaacct
     2641 ggtcgcttgc ttcatcggcg tcctaatcgg gacgctggtg ggcatcttac cggggctagg
     2701 gcccgccgcc accctggcta tgttgttgcc aatgaccttc gggatgggcg ccacaacctc
     2761 catgatcatg ctggccggtg tgttttacgg gtccaagtat ggtggcgcta tcacctcggt
     2821 actggtgaac ctgcctggtg agtcctcctc ccttatgacc tgcatggatg gctatccaat
     2881 ggcgcggaaa gggagggccg gccctgccct cgggatgtcc gccatcgcat cgttcttcgc
     2941 gggtacagtt ggagtgatcg gcctgatgct ggtggcgccc ccgttggccg acttggcgct
     3001 gaatttcgga ccgccggaat actttgccct gatgtgcctg gggttgacgc tggtgacctt
     3061 cctggccgga aaatcgatgg tgaaggccct gataatgggt tgcgtcgggc tcatactttc
     3121 cacggttggc tcggatacga tgtcgggcag ctacaggctg tggttcggcc agcccttcct
     3181 gctgggcgga gtagacttcg tcgtcgcggc aatggggctc ttcgccgtcg gtgagatcct
     3241 gatgacgatc gaggaagaag cggctgtgga gaccattccc tcccccaaga agctctcaga
     3301 actattgccg tcctttcacg acataagaga atgctggtgg accctggtca ggagcacatt
     3361 gctaggtttc ctgattggta cggttcccgg cggcaatcct accatcgcca ccttcctttc
     3421 gtatggagcc acgaaggcaa ttgcgaagga ccctgaggtg tttgggtccg gcactatcag
     3481 gggggtggcg gcgtccgaat ctgccgacaa tgcagccacc agtgggggaa tggtacccct
     3541 gctgacgctg ggcgtgccgg gcacggcatc cacagcgata ctcctcgggg cacttatcat
     3601 ggcgggcctg cagccgggtc ccctgctgat gcagcaggag ccagagctgt tctgggcaac
     3661 gatcgccagc atgtacattg gcaacgtcct tctgctgatc atgaatctgc cgctggtgcc
     3721 agtctttgcc agccttatca gggtcccgta cttcgtgctc tatcccatga tcctcgcgat
     3781 ctgtagcgtc ggcgtataca gcctggacaa caaggtgtcc gatgtggggc tgatggccgt
     3841 gttcggcatc attggttacc tgatgaagaa ggcggagttt ccagccgctc cgttggtcct
     3901 tgcgctggtg ctgggtggaa tgatcgagaa gagcttgcgg cagtcgctga ccctctttga
     3961 gggggacttg accgtgtttt tccagcggcc gctctgcgca gtcctgctca ccttgacggt
     4021 ggttatgcta ctgcttcctg tggtgcgagg gactcgaaag aggcctgctc cggtgccgca
     4081 gcagtaggac cacagtcgat ggcgagagcg atggtttgga ggagcttcag atggtagaaa
     4141 ccggtacggt gcgcgccctg ctccagggca gctacgatct gcacgtccac agcgccccag
     4201 atatcatgaa gcgaaagggg agcgacctgg taatggccag gagggcggct gccgcgggta
     4261 tccaggggat gctgttcaag tctcacaact catctacggc cgatcgggtc gcggtggtca
     4321 acgagactgc gcctggttgc cgtgcttacg gcgcgctgac tctgtgtcac gccgttgggg
     4381 gtctgaacgc tgctgccgtg gatgtcgctg cacgattggg agcccgcttg gtctggtttc
     4441 ctaccatgga tgcggcgaac gaggtggcat atcaggatgc tcacccgaac gggaagaagg
     4501 cctactggtt cgtggcgcag caagagttgc ggcaacagag ggtctctcgg ccccccatct
     4561 ctatcattga tggggatgga gccctcgttc ctgccgttca cgagatcctg gaggttatcg
     4621 cggcccacga tatggtgctc gccacgggcc atctgggcgt tacggagata gtcaggctgg
     4681 tggccgccgc gaaggccgct cgagtgcagc ggatcatcgt ctcgcatccg gagtacccgt
     4741 cggttgccat gccgatcgtt ctgcaggtgg agttggcgcg gcagggcatg ctcatggagc
     4801 gctgctatcg gactccctac tcaggcaaga taggctggga agaacagctt gctgccatcc
     4861 gcgccgtcgg cccgtccgca tcgatagtct gctcagacat ggggcaggtc gacgcgccgt
     4921 ggccggagga ggcgctattc gatttcgtcc agcggttgct ggaagcaggg ttcacaacgg
     4981 aagaggtgcg agtgatgaca gccgacaacc cccggagtgt catcgaactg tagggcgcca
     5041 tcgaccagga tggacctggc ataacacgca tttggaggga aggtgtaatg ccctacgctg
     5101 atatcggcgg ctactcgatg tactacgagt tggacaactt cacagatccc tggaagcagc
     5161 ccgagaccgt gttgctccat gcgggctact gccgcaacag ccgattctgg tacgcgtggg
     5221 tgcccggtct ggcgcgacac tatcaggtgc tcaggcccga ccaccgaggg agtggggagt
     5281 caccttcccc ggggcccgat ttccaaccca ccatcgaggg atttgtcgac gacgtgatgg
     5341 cgctgatgga ccatctgcgg ctcgagcggg tgcactacgt gggtgagtcc ttcggcggcg
     5401 tggtgggcat tcagatggct ctcacccatc cggagaggct gcacagcctg accctctgca
     5461 acaccgggtt ccggatactc cccaaagcgc tgaccgccaa ttccgcggga ttcccagaca
     5521 gactggacgc gatcgacgag ttgggtctgg agggctggtg ccgcaagacc atagacgtga
     5581 ggctcgatca ggagagggct tccgcagagc tctccaactg gtactgccag gagatcggcc
     5641 gggccgatcc actgctggcg aagcggctga gcatggttac caggtatgtg gatttctcgc
     5701 cgcggatggc agagatcaag gtgccgacac tccttctggc tcccggaaag agcaagatta
     5761 ccgacctcga gcagcagcga ttcatggcga acgccatccc ggatgccaag ctcgtggtga
     5821 ttgagaatgt tggtcatggg gtcaacgtgc ttgagcccga gcgatgcact ttggagctgt
     5881 tggacttcct cgccggccga aaggcaggtg ccagccaggc gtagcggcgc atcgaggccg
     5941 atgaccgttt ctcgcatctc gagacggacg gcggatacaa caacccgccc cgcatggacc
     6001 tgctcttgga caggtccatg tgcggggcgg ttttcgagtt cccccgggct acctggggga
     6061 gtggaggata gaagtagtgg ctacaaggca ggtcaagtgc agcatcatac gtgggggcac
     6121 cagcaagggg gtgttcgtcg agagagatga gctgcccccg catggaccgg agagggacaa
     6181 gatcatcctg gctctctacg gtagccccga caagcgacag atcgatggtc tgggtggcgc
     6241 ggacaagctg accagcaaga cggccatcat gggcgccccc acgcgctccg actgcgacat
     6301 cgattaccac tacggacagg tcggcaccga cctgccgact atcgattgga ccagcaactg
     6361 cgggaacatc tctgcgggcg ccgccgcata tgcggtgtac aagggctacg tgcgggccac
     6421 ggggccgtcc gccctagttc ggattcatca ggtgaacacc gggcggaggc ttctggctac
     6481 ggtgcccatg gccgatggga tgcccgcgac cgaaggggac ttcaagatcg gcggagtccc
     6541 cgggaccggg gccaggatcg acctggattt ctcggatttc acgggatgca tactcaacag
     6601 ggggttactg cccactggct ctcctatgga ccggttcaag gtgcctggga ttgggatgct
     6661 ggatgtgagc gtggtggatg tcgccaatct gtgcgccttt gtccgtggca gcgacgtggg
     6721 aatggataac accgaagata tcgagtccct gcaggccaat caggagatcg tggcaaagct
     6781 ggaggctatc cgggcggtgg tggccctgga gacgggcctc gtgagcagag acgtcgacac
     6841 cgaccgcgag ctgaggcggc gggtcaaccc gttgatgttc atcgttggca ctccgagaga
     6901 ctacgatgcc ttgaacggcg agcatatcga tgcggggaac accgacatct tcgctcgcag
     6961 ccttgcccgc tgggccttct ccaagaccta ccctgccacc ggatccatcg gcacgggcgt
     7021 ggcttgcacc gtggctggca gtatacctgc cgaagtcgta cgtggtgggc cgccggcacg
     7081 tggcgagact cgctcagtgc ggattgggca tcccagtggg acactggtta cggaggctgg
     7141 catctctgca gatgggcagt tggtgatccg cggctcggtg ggccggactg cgcgggtgct
     7201 gatggaaggg acagccttcg tcagggtgta gagatagagt gtagagatgg ggtgagtggc
     7261 cgtcgccctc gaccgtcgag agcgtcggcc actctgtttg tctagcggtt cttcgccatc
     7321 tgggcggcca gcctcagcgc ggccagcatg ctggtcggcc tcgccgtgcc cttgccagcc
     7381 ttcccgaaca ccgtgccgtg gtccacggaa gtccggatga tcggcagccc cagcgtcacg
     7441 ttcacgccct cgtcgaaggc gattaccttg gtggggatgt gcccctggtc gtggaacatc
     7501 gccaccaccg cgtcgtactg accctcgtgc atccggaaga acacggtgtc cggcgggatg
     7561 gggccgacca cgttgaagcc ctcggccttc atcgcctcga tggccggcgt gatctccttg
     7621 atctcctcgt cgccgaatag tcctccctca ccggagtggg ggttcagccc agacacggcg
     7681 atcctcggct ccgccacgcc cagcatcttc gtggcctggt gcgtcatccg gatcacgtcc
     7741 atgatccgcg gcttctttgc ccgctcgatg gcctgtcgaa gggagcagtg ggtggacacg
     7801 tgggttaccc tgaagttgcc ggccaccagc agcatgcaga cgctcttgat gccgcacagg
     7861 tcggccagga tctcggtgtg cccgtcgtag tggtggccgg cgaggttcag cgactccttg
     7921 ttgatggcgg aggtcactat cgccgcggtc tcgcccgcct tggccagctc gacgcccttg
     7981 acgatggatt ggaaggctgc ctctccagat gcagcctgaa ccttgccgta ctggagttgc
     8041 gataggtcga cgttgtgcag gtctagcact tcgaggaccc cgtcctggaa ggtggcatcc
     8101 gccactcgct cgatggatct gacctgtaca gtggatccca cgatgctcgc ggcccgctct
     8161 atggtcgctc cgtcgccgat cgctatgggc cgagacgcgg cgcgaacttc gggcgtggtc
     8221 agggccttca ccaggatctc tggacccgac ccgctgggat cgcccatggt gacgcccagg
     8281 atcggcctgc tgtcgctcat gatagtctcc cttgtagaca gtgtatcgcg gctattatag
     8341 ccttttcgtc cccaaaccct ccggccttgg tcaccaccct gaggccgtcg gacctgccgc
     8401 ctaccagaac gcccgcgggc acgcccggct gcacctcgcc cagtgcccgc agccccgtcg
     8461 cgccgagccg gaggcacacg gccctcgcga tgtctccccc agtcatgaat acgcccgata
     8521 cctcgtgcat ctccagcagc ctggccgtgg cgtcagcgag cctctgcgcg ttcgccatcc
     8581 cctgcccctc ctggtagggc gcgaaggtgg tggtgatggc cacgttgtgc ctccggccca
     8641 gcagctctgc cgacttcacc cccaccagtt caccccaatc ggcgtcggac ggtagatcca
     8701 ttatctccgc cagcccccgg cgggatgctt cctgaagctg cgccgcggtg gtgcggttgc
     8761 ggctccctgc gactaccagc acgggccgcg agtcggcaga ccagccgatc ccgccctcgc
     8821 tgcttcgagt cgcccccagc gcggcgatcc actcctcggc cagccccgcg caacccgccg
     8881 gcagccagag ccgatccatg cgcaccagcg cctccgccag cgttcgcagg tggcgagact
     8941 ccacggcgtc cgcagccacg atgggcgcct tctcacgctt cagcgcctca atcacttcct
     9001 cgggaccctg ctccactacc tccagcgaga ggtggccgac ggcacgacgg gtctgccgtg
     9061 cgatgatggt gggaaggtgg gattccgtgg ccggccacac cgggtcgcgg gcgaaggcgc
     9121 tttcggagag gaggacgccg tggaccttgt ggtagccgcc ctcggtagtg cggccggtat
     9181 tggggaaggc cggagcgacc agggctcgtt ccagccgcag gccgtccagc atgccgtcga
     9241 tctccgcccc caggttgccc cgcagggtgg agtccatctt cttgtacacc agccgcccgg
     9301 cgagccgtcc ggcggcctct cgcgcgtggc ggtaggcgac ctcggcgggc tcgtcccggc
     9361 tgtcggtgct gatcactacc atctctgccg gcggcagctc gccgttgccc agcatcacga
     9421 cggcgcgcag ccccgccttg gcggactgga cgccggtgtc catggctccg gttaggtcgt
     9481 cggctacaat gccaagttgt gtcattacgt tagcccaagg gtggagtgct ggcccagggg
     9541 gccgggtgtc gggtaccagg gaaatggagt cgagcaacgc cgtcgatgcc ttacccgacc
     9601 cccaacccct ggcccccggc ccccgatcag atc
//
LOCUS       DATALS010000390         3459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_335771, whole genome shotgun sequence.
ACCESSION   DATALS010000390 DATALS010000000
VERSION     DATALS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335771"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1512
                     /locus_tag="VHS06_08755"
     CDS             301..1512
                     /locus_tag="VHS06_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988244.1"
                     /translation="MAQVGQGGVMAIFAERESVSTSGVTATESVEHTWPTSLPSARVL
                     LVALLLLSLAFSVSVIFRAQNPYGTLEPKYTDHLRHERYAEELLHRGLGLYSEPAGQL
                     TRFGWEDDRTYIWPEVSYPYPVGALLFHLPAGVAIYRLGVNPVLVNQLVIVAYVALAH
                     IGVFLFAREWLLTGRGGNIFIGLWLVAAFWALVVVWSLNAQFEALPLLFPLLAARRYW
                     QGRHLEAALWVGVALFFKYQAAIFAPLVLGMLWQARSQGWRTLWRPGLVALMGVVGLD
                     LYTTVISYHFVQIPEANLVSLDALLAGRWVPTGFRLLATATLTVSLTGLLAWRRQWML
                     AASAAFAVLFLASLPQVQGWYVLWLFPLALFGRGTDRDSVGFWTLGLCYLIYQLPDPA
                     YLAGAFAPFFH"
     gene            complement(1703..2344)
                     /locus_tag="VHS06_08760"
     CDS             complement(1703..2344)
                     /locus_tag="VHS06_08760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2988245.1"
                     /translation="MATGLTGEIISRLSGRGYHQFMHEEILEPLGMRDSYMLPPEEVW
                     ERIATVWAPNEPHTDYERYNSPYFRKLGIPWGCLYTTPEDLALFGQMFLTEGRSVGGR
                     IISPATAREMTRDQLRGVPGGIASSSERWEEASWGLGWDVKGNKAPHSTGALTSPRTF
                     SHGGSAGSLMWCDPVTDVICVLIANRTLESDWTTLCEPRQALFSNAVAAAVVD"
     gene            complement(2313..2846)
                     /locus_tag="VHS06_08765"
     CDS             complement(2313..2846)
                     /locus_tag="VHS06_08765"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX2988246.1"
                     /translation="MLPTCKPEEVGLSPVRLERAFDLLRGWTDDGTLPGVTALVARRG
                     KLAGRFFASWASREPSPKPVEADTIFAVASITKPFTATGLMLLVERGLVSLDQPVYTV
                     LPEFAGPTRDGITLRHFLTHTSGLPEYAPNNEEIRRRHLGLDAFVEVYCQGEPTFEPG
                     SRHSYSNYGYRADGRDH"
     gene            complement(2849..3388)
                     /locus_tag="VHS06_08770"
     CDS             complement(2849..3388)
                     /locus_tag="VHS06_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX2988247.1"
                     /translation="MKNVWQGRLKAPKFIADSNVGKLARRLRMLGFDALYIHPIADDL
                     LVEIAQLENRVILTRDTYIFHRRVVFDGQVPAVMITHDDVRDQVRQVLRELVLGPPFP
                     LFSRCLECNAPLRPLGREQAESLVPPYVYRTQREFTICPRCGRVYWAGTHRQHMLEEV
                     ESLGGEPDGTLPPEETEEE"
BASE COUNT          562 a         1096 c         1147 g          654 t
ORIGIN      
        1 accattggga cgagccacca gaaacagaaa ggctcgggtg gtggcgagaa aggcgaaagt
       61 caggaggaag taacggtcgc gggtgagcga ggctagcctg ggacgtgctg gcgcagttgg
      121 gactggggca gggcgcacct ctagcacggc cttcccgttg ggtggaaaca ctctctggca
      181 cctccccagc cactgcccag gccggccgcg aagcagccgg cgtcgctcgc gccagagatg
      241 caggctctat gccacttgag atggtgagtg ggctggttta catcctgcga aggtgacgcc
      301 ttggctcagg tgggccaggg cggcgtcatg gccatcttcg cagagaggga gagcgtgagc
      361 acttcgggag tgacagcgac tgagagcgta gagcacactt ggccgaccag tttgccctcg
      421 gccagagtac tgctggtggc gctgctgctc ctcagtctgg ccttctctgt gagtgtgatc
      481 tttcgcgctc agaatcccta cgggacgctg gagcccaagt acaccgatca cttgcggcac
      541 gagcgatatg ccgaggagct cctccatcga ggtctcgggc tctacagcga gccggccgga
      601 cagcttacgc gctttggctg ggaagatgac cgcacctaca tctggcctga ggtctcctat
      661 ccttacccgg tgggcgccct gctgttccat ctgcccgccg gcgtggccat ctatcgcctt
      721 ggcgtcaatc ccgtattggt caaccagctg gtgatcgtcg cctatgtggc cctcgcccac
      781 ataggcgtgt ttctgttcgc ccgcgagtgg ctgctcacgg ggcgcggtgg caatatcttc
      841 atcggactct ggttggtggc ggccttctgg gcgctggtcg ttgtctggag tctgaatgcg
      901 cagttcgagg ctctgccgct gttgtttccg ctgttggccg cccgacgcta ctggcaggga
      961 cgccaccttg aggcagccct ctgggtcggc gtggccctgt tcttcaagta tcaagccgcc
     1021 atcttcgcgc cattggtgct ggggatgctc tggcaagcgc ggtcacaagg ctggaggaca
     1081 ctgtggcggc cgggactggt tgctctgatg ggagtagtgg ggctagacct ctacaccacg
     1141 gtcatcagct accacttcgt gcagattccg gaggccaacc tggtaagcct ggacgctctg
     1201 ctggccggca gatgggtgcc cacgggcttc cgattgctgg ccacggccac cctgacggtc
     1261 tcgcttacgg ggctgctggc ttggcgccga cagtggatgc tggccgccag cgcggccttc
     1321 gccgtgctgt tcctggcttc tctgccccag gtgcagggct ggtacgtgct ctggctcttc
     1381 cccctcgctc tatttggccg ggggaccgac cgagactcgg tcggcttctg gaccctgggc
     1441 ctgtgctatc tgatctacca gctgcccgac ccagcctact tggcgggggc gttcgcaccg
     1501 ttctttcact agagaccatg tcgatttgcc aagcgtcttt acctctcccc cggcccctcc
     1561 tcccgctgcg cgggactacc gcctggcagg ggaggggaga ctaggtaggg cttggtccac
     1621 ccttcccagg cagagaacgg gatcaacggg taggtgaaga acgtctggcc agcgagaacc
     1681 aactcgcctt agttgatcgt cgtcaatcca ccaccgcggc cgccaccgca ttggagaaga
     1741 gagcctggcg gggctcacag agggtggtcc agtcgctctc cagcgtgcgg ttggcgatca
     1801 gcacgcagat cacatcggtg acagggtcgc accacatgag tgacccggcg gagccgccgt
     1861 ggctgaaggt ccggggggag gtgagtgcgc cagtgctgtg cggcgctttg ttgcccttga
     1921 cgtcccagcc cagtccccag cttgcctcct cccagcgctc gctggagctg gcgatgccgc
     1981 ccggcacccc ccgcagctgg tctcgcgtca tctcgcgcgc ggtggcgggg gagatgatgc
     2041 gaccgccgac cgaccgtcct tcggtgagga acatctgccc gaagagagcc aggtcctcgg
     2101 gcgtagtgta gaggcagccc caggggatgc ccagcttgcg gaagtagggg gagttgtagc
     2161 gctcgtagtc ggtgtggggc tcgttcggcg cccagaccgt ggcgatgcgc tcccagacct
     2221 cttctggagg cagcatgtag gagtcccgca tgcccaaggg ctcgaggatc tcctcgtgca
     2281 tgaactggtg gtaaccccgg ccgctgaggc ggctaatgat ctctcccgtc agccctgtag
     2341 ccatagttgc tgtaggagtg gcggctgcca ggctcgaagg tcggctcgcc ctggcagtac
     2401 acctccacga aggcgtccag ccctaggtgc cgcctcctga tctcctcgtt gttgggggcg
     2461 tactccggca agccagaggt gtgggtgagg aagtgccgca aggtgatgcc atcccgagtg
     2521 ggaccggcaa actcgggcag aacggtgtag acgggttggt cgagggagac gagcccgcgc
     2581 tcgaccaaga gcattaggcc agtggcggtg aagggcttgg tgatggacgc caccgcgaag
     2641 atggtgtcag cttccaccgg cttggggctg ggctcgcgcg aggcccagct ggcgaagaag
     2701 cgccccgcta acttaccgcg tcgggccacc agggctgtga ccccgggaag cgtaccgtca
     2761 tcagtccagc cacgaagcag gtcaaacgct cgctccaggc gcacggggga gagcccaacc
     2821 tcctccggct tgcaggtcgg cagcattctc actcctcctc agtctcttcc ggcggcagag
     2881 tcccgtcagg ctctcctccc agagactcta cctcctccag catgtgctga cggtgcgtcc
     2941 ccgcccagta gacgcgcccg cagcgggggc agatggtgaa ctcgcgctgc gtgcgataga
     3001 cgtagggagg caccaggctc tcggcctgct cccggcccaa gggccgcagc ggcgcattgc
     3061 actccagaca gcggctgaag agaggaaagg gcggccccag aaccagctcc cgcagcacct
     3121 ggcgcacctg gtcccgaacg tcatcgtgag tgatcatcac ggccggcacc tggccgtcga
     3181 agaccacccg gcggtggaat atgtaggtgt ctcgcgtcag gatgacgcgg ttctcgagct
     3241 gagcgatctc gaccagcagg tcgtcggcaa tcgggtggat gtagagcgcg tcgaagccaa
     3301 gcatgcggag ccgccgggca agcttgccca cgttgctgtc cgcgatgaac ttcggcgctt
     3361 tcagccttcc ttgccagacg ttcttcacct acccgttgat cccgttctct gcctgggaag
     3421 ggtggaccaa gccctaccta gtctcccctc ccctgccag
//
LOCUS       DATALS010000391         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_43608,
            whole genome shotgun sequence.
ACCESSION   DATALS010000391 DATALS010000000
VERSION     DATALS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43608"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VHS06_08775"
     CDS             <1..698
                     /locus_tag="VHS06_08775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX2988248.1"
                     /translation="VTPEDAPGLHRLVDLLSLQARLPKPRVYIMESNTPNAFATGRDP
                     AHAAVAVTTGIQRILSADELSGVLAHELAHIRNRDTLISAVVATMAGAITMIANMAQW
                     AMMFGGFGRGEDEDEEGGMAGLAGSLLMIILAPIAAAVVQLAISRAREYNADSMGARI
                     LGDPLPLARALEKLEIANQALPMAVKPATAHQFTVQPLSGQGLAGLFSTHPPIAERVS
                     RLSRMALQPLSPL"
     gene            859..1077
                     /locus_tag="VHS06_08780"
     CDS             859..1077
                     /locus_tag="VHS06_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX2988249.1"
                     /translation="MQCPICDVNLLTTDRHGVEIDYCPHCRGVWLDRGELDKIIKQAT
                     ETARERYATQRAWPKLNSQPGIQAVGDV"
     gene            1403..1726
                     /gene="metG"
                     /locus_tag="VHS06_08785"
     CDS             1403..1726
                     /gene="metG"
                     /locus_tag="VHS06_08785"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEX2988250.1"
                     /translation="MITIDDFAKIELRVGTVVSAESIKGATKIMKLEIDIGDRKVVTT
                     AGIAQHYSPEEMMGRRVVVVANLEPRMVRGIESQAMILAASSEGHKTVLVTVAEDIPN
                     GAKVK"
     gene            1767..2510
                     /locus_tag="VHS06_08790"
     CDS             1767..2510
                     /locus_tag="VHS06_08790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2988251.1"
                     /translation="MPRVSVVIRAKNEAQYIGETLAAVQGQQYRDFEVIVVDSGSTDR
                     TLDIAEEYGAKIVTIDPGEFTYGGALNLGIANSQGQLLVSLSAHATPETPYWLGSLVR
                     GFGSPSVAGVYGRHIPRENVSFFELLGMNLSGVTSKEPRLQTGSARFSNANGAVRRDL
                     WELHPFNEGLPGAEDIDWARQMQHLGYVIAYEPRAAVYHSHGESLPRLIKRQLHDQPV
                     IARAWAGGMIYGYTNRRKTVVGRRELPSK"
BASE COUNT          629 a          881 c          891 g          522 t
ORIGIN      
        1 aggtcacacc agaggatgcg cccggactgc atcgtctggt ggacctgctt tcgctgcagg
       61 ccaggctacc gaagccgagg gtgtacatca tggagagcaa cacgcccaac gcatttgcca
      121 ccggccgtga tccggctcac gcggccgtgg ctgttaccac cggcattcag cggatactgt
      181 ctgcggatga gctatcaggg gtgctggcgc acgaactggc gcacatccgg aaccgcgaca
      241 ctctaatctc ggcagtggtg gcgaccatgg ctggggccat caccatgatc gccaacatgg
      301 ctcagtgggc catgatgttc ggagggttcg gccggggcga agacgaagac gaggaagggg
      361 gcatggccgg gcttgccgga agcctgctga tgatcatcct cgctccgata gcggccgccg
      421 tagtccagct ggcgatatcc agggcgcgag agtacaacgc cgacagcatg ggagcccgaa
      481 tcctgggcga cccgctgccg ctcgctaggg cactggagaa actggagatc gccaatcagg
      541 cactcccgat ggccgtgaaa cccgccacgg cgcatcagtt cacggtccag cccctatcgg
      601 gccagggcct ggcaggactg ttcagcaccc atccgcccat cgcagagcga gtatccaggc
      661 tcagccgcat ggctctccaa cccctgtccc cgctctgagc agctttcact ggatgcagtc
      721 cacctttcca tgagggaaag gtggatacca acaagggaaa ggcgctaggt gaacgttggg
      781 ctgccggcga accggcagcc cgccaaccat cccgtagtca ttcctcaagg gaatcgacct
      841 ggagggtaga acagaatgct gcaatgccca atatgcgacg tgaatctgct gacgactgac
      901 cgacacgggg tcgagatcga ctactgccct cactgccggg gcgtatggct ggacaggggc
      961 gaactggata agatcatcaa gcaggcaacg gagactgcac gagagcgtta cgccacgcaa
     1021 agagcgtggc caaaactgaa ctcgcagcct gggatccagg ctgttggtga tgtataatag
     1081 gtcgtggcag cttccccacg cggcaatggc gccgcacata ccacgcgcta ggtggctctg
     1141 ccaccaaata gggggaacta cgacccatgg gcatagtagt cgccctgagg gactcggccg
     1201 atggacgaga acgggttctt ctaggccgaa acggtcggtc agaagttgac ctggcgctga
     1261 tagccgacag gccgagcacc cttcctttcc ctggacacga gggcagcgac gaaccgatcc
     1321 ggtacgccgg cagatgctcc gtctccagcg gcaacgagcc ctcggttcgg cctcttcttc
     1381 tacacactgc aggagtcctc aaatgatcac aatcgacgat ttcgccaaga tagagctgcg
     1441 agtaggcacc gtggtgagtg ccgagtcgat caagggtgcc acgaagatca tgaagctcga
     1501 gatcgacatc ggggaccgga aggtcgtcac caccgccggc atcgcgcagc attacagccc
     1561 cgaggagatg atggggcgac gggtagtggt tgttgccaac ctcgagccga gaatggtccg
     1621 agggatcgag tctcaggcga tgatcctggc cgcctccagt gaggggcata agaccgtact
     1681 ggtaaccgtg gcagaggaca tccccaacgg agccaaggtc aagtaggtcc atgccccgca
     1741 ttatcgttcg ttacaggtga gtatcagtgc ctagagtgtc cgtggtcatt agggccaaga
     1801 acgaggctca atatatcggc gagaccctgg ccgccgtgca gggacagcag taccgcgact
     1861 tcgaagtaat cgtggtggac tcaggctcga ccgataggac cctcgatatc gccgaagagt
     1921 atggtgcgaa gatcgtgacc atcgatcccg gcgagttcac gtacggcggt gccctcaatc
     1981 tgggtatcgc caactcgcag ggacagttgc tggtgagcct gagcgcccac gcaacacccg
     2041 agactcccta ctggctggga agcctggtcc gcggtttcgg gtccccgtcc gtagcgggcg
     2101 tctatggaag gcacattccg agggagaacg tctctttctt cgagttactg ggcatgaacc
     2161 tctccggcgt taccagcaag gagccaagac ttcagaccgg cagcgcccgc ttctccaacg
     2221 ccaatggtgc cgttcggcgc gatctctggg aactccatcc cttcaacgag ggactgccgg
     2281 gtgccgagga catcgactgg gcgaggcaga tgcaacacct gggttatgtg atagcctacg
     2341 agccgcgggc cgccgtctac cactcccacg gagagtcgct gcccagactt atcaagcgac
     2401 aactccacga ccaaccggtc attgcacgcg cttgggcggg tggcatgatc tacggctaca
     2461 caaatcgtcg aaagaccgtg gtcggacgtc gagagctgcc ctccaagtag gcccaatccg
     2521 acaagtgacc acccagggtc gctccatttc gctaggggcg gcccttttcc tttccccggc
     2581 gaccggcggc gaactccccg gcagccgtcc tttcggacag aaaacccgtc tgttacaatt
     2641 tatatctggt aaagtattcg ccgcggcatg ttcagcactg tgtaacgccg acaaagtacg
     2701 cgttgtgacc tctggcaacg cctgaagtac cgacggcgcg gctcatgcca atatccgccg
     2761 cagatggcgc aaatcctcac caatcgggtc cagtcatgag agagatcgcg gcagggcaat
     2821 cagagaagga tctgattcgt tggctcacag ggctcactgt agccctggtt gtgcttcacg
     2881 ctattctgct cctcatttgg ggcagagggg catccctcat ggt
//
LOCUS       DATALS010000392         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_425788, whole genome shotgun sequence.
ACCESSION   DATALS010000392 DATALS010000000
VERSION     DATALS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425788"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VHS06_08795"
     CDS             complement(<1..151)
                     /locus_tag="VHS06_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX2988252.1"
                     /translation="MKVVSVVGARPQFIKAAPVSRALRAAGHLEVLVHTGQHSDERMS
                     EIFFSE"
     gene            343..1479
                     /gene="wecB"
                     /locus_tag="VHS06_08800"
     CDS             343..1479
                     /gene="wecB"
                     /locus_tag="VHS06_08800"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX2988253.1"
                     /translation="MVILSVVGARPQFIKAAPVSRALRAAGHLEVLVHTGQHYDERMS
                     EIFFSELEIPAADYNLGVGSGSHGLQTGRMLMLLEEVLVGESPDWVLVYGDTNSTLAA
                     ALAAVKLQIPLAHLEAGLRSYNRAMPEEHNRVLTDHCSDMLLCPTRTAVDNLAREGIT
                     RAVHLVGDTMYDAVLQHCQVAGQLSNILDREGLAPKGYLLATVHRPYNTESPEALRDI
                     VLALEEIGDPVIFPVHPRTRQRISKLEGLAALAASLSHVRMIEPVGYLDMLMLEQNAR
                     LILTDSGGMQKEAYFFGVPCITLRPETEWVETVETGRNVLVGANRGRIVEAASVDYGS
                     HTDSPPLFGDGRASHSVVRLMEEGCPIVGDESGDRSPMHMVRVS"
     gene            1476..>1979
                     /locus_tag="VHS06_08805"
     CDS             1476..>1979
                     /locus_tag="VHS06_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2988254.1"
                     /translation="MKTVLVTGVAGFIGSNLAEALLSLGYRVIGLDNLSQGSTQNIAG
                     FLASPRFEFHRGDVRDSREVEGLIGRADCVMHLAAYKIPRYGNALDTIEINTQGTRNV
                     LRSAAKLHRLVVFASTSDVYGRNPDVPFHEGSDLWMGPCNVPRWSYAISKMFDEHLCF
                     AFQREFGL"
BASE COUNT          401 a          549 c          610 g          419 t
ORIGIN      
        1 gttcactgaa gaagatctcc gacattctct cgtcggagtg ctggccggtg tggacgagca
       61 cctccagatg gccggcggct cgtaaggctc gactcacagg tgcagccttg atgaactgcg
      121 gacgggcgcc tacaacagag acgactttca ttacacgccc cactccttga agccgcttca
      181 gttgatcccc agcgaagcca cgtgcctggt tccagccaat gaggcaacgc cgcgtttgcc
      241 aatgccgaga tagcgcactc cgagacgctg gattagcgca ggatccagca cgttgcgacc
      301 atcaaggacc actcgcaggc cccgaaacag accataatcc agattgtgat attgtcggtg
      361 gtaggcgccc gtccgcagtt catcaaggct gcacctgtga gtcgagcctt acgagccgcc
      421 ggccatctgg aggtgctcgt ccacaccggc cagcactacg acgagagaat gtcggagatc
      481 ttcttcagtg aactcgagat tccagcggcg gattacaacc ttggcgtggg atctggttcc
      541 cacggattgc agactggtcg gatgctgatg ctgctggaag aggttctggt gggagagtcg
      601 cctgactggg tgctggtcta cggcgacacg aattccaccc ttgcggcggc actcgcggcc
      661 gtgaagctac agatccccct cgctcatctg gaggccggac tacgatctta caacagggcg
      721 atgcccgagg agcacaacag ggtgctcacc gaccattgct cggacatgct tttgtgtccc
      781 actcggacgg ccgtcgataa cctggcaagg gaagggatta cgcgggcggt gcacctggta
      841 ggcgacacaa tgtacgacgc cgtgcttcag cattgtcagg ttgccggtca actgtccaac
      901 attctggacc gagaggggct ggcccctaag ggctacctgt tggcgaccgt tcatcgtcca
      961 tacaacactg agagcccaga ggctctgcgc gacatcgtac tggcccttga ggagataggt
     1021 gatccggtca tattcccagt ccatcctcgc acgaggcaga ggatttcgaa actggaaggt
     1081 ctggccgcgt tggccgcctc cctgtctcat gtcaggatga tcgaaccggt cggctaccta
     1141 gatatgctga tgttagagca aaacgcgaga ctgatcctga ccgactccgg cgggatgcag
     1201 aaagaggcct atttcttcgg tgtgccctgt atcaccttgc ggccggagac cgaatgggtg
     1261 gaaacggtgg agacagggcg aaacgtgttg gtgggagcca atcgagggcg cattgtggag
     1321 gctgccagtg ttgactacgg ctcgcacacc gactcgccgc cactatttgg cgacggacgg
     1381 gcgagccatt ctgtggtgcg gttgatggaa gaggggtgcc cgattgttgg agacgagtca
     1441 ggcgatcggt ctcccatgca catggtgaga gtctcatgaa gaccgtactc gttactgggg
     1501 tagctgggtt tattgggtcg aatctggccg aagcccttct tagcctcggt taccgtgtga
     1561 ttggactgga caacctgtcg cagggctcca ctcagaacat cgctggtttc ctggcgagcc
     1621 cgaggttcga gtttcacagg ggtgatgtca gagactcgcg agaagtggag gggctgatag
     1681 gacgtgccga ctgtgtgatg cacctggcag cctacaagat tcctcgttac gggaatgcgt
     1741 tggataccat cgagatcaat actcaaggca cgcggaacgt tcttcgatct gccgcgaagc
     1801 tgcaccgtct ggttgtcttt gcatctacgt ccgacgtcta cggccgcaat cccgacgtcc
     1861 ctttccacga gggcagcgac ctctggatgg ggccgtgtaa cgtgccacgc tggtcttatg
     1921 ccatctcgaa gatgttcgat gagcatctgt gctttgcatt ccagagggag ttcgggttg
//
LOCUS       DATALS010000393         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_425884, whole genome shotgun sequence.
ACCESSION   DATALS010000393 DATALS010000000
VERSION     DATALS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425884"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VHS06_08810"
     CDS             <1..801
                     /locus_tag="VHS06_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX2988255.1"
                     /translation="IGLIGFGAIGRTVARAVAAGAAGDTTIVAVLVRDPAKYEVESTG
                     YPYSFYGDPDLFFDSGMDMVVEVAGHDALRRYAERALRSGMDYLTVSVGAFADDELLE
                     RVRSAATASGRRVLIPSGAIGGLDAISAGAIGKLDEVTITSRKPPVAWKGTVAEREVD
                     LDAVTEPVCLYEGPARKAAALYPQNVNVQAALSLAGIGMDLTRSRVFADPSVIQNTHE
                     ILARGAFGEVRISISNIPSDESPKTGRITAMSVTKAIRNLTAPIVVGL"
     gene            complement(867..1616)
                     /locus_tag="VHS06_08815"
     CDS             complement(867..1616)
                     /locus_tag="VHS06_08815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2988256.1"
                     /translation="MEIRGKTAIVTGGTGGLGRRICLTLAKAGANVALVYLKSQTEAE
                     GFAAELCSLGPQVIAIQADITSQAGIDTMIQTTLRKFGRVDALVNDAAYNRFIAYQDL
                     DTLDEDVWNHILHSNLTSPFLAMRAIAGAMTGEDGGRIVNVASIAGLNPSGSSIAYAV
                     SKAGLIHLTRCMAVALAPRVLVNCVAPGFLEGTRMSDNLPPEFQERARQSSALRKAAD
                     KDDVADAVLTLIRTNSITGQTLVVDAGRVFH"
BASE COUNT          296 a          497 c          564 g          332 t
ORIGIN      
        1 atcggactga tcggctttgg agccatcggt aggacggtgg ccagagctgt tgcggcgggc
       61 gccgcggggg acacgaccat cgtcgcggtt ctggtgcgtg accctgcaaa gtacgaagtg
      121 gagtccaccg gttaccccta tagcttctac ggcgatccgg atctcttctt cgacagtggg
      181 atggacatgg tcgtcgaggt ggccgggcac gatgcgctgc gacgctacgc cgagagagcg
      241 ctgcggtcgg gaatggacta cctgaccgtg agcgtcggag cattcgcgga cgacgaactt
      301 ctggagaggg tgaggagcgc tgccacggct tccggtcgca gagtgctgat cccttcgggg
      361 gcaatcggag ggttggatgc aatctcggcc ggggcgatcg gcaagctgga tgaggtgacc
      421 atcacctcgc ggaagccgcc cgtcgcctgg aaggggactg ttgccgagcg agaggtcgat
      481 ctggatgccg tgacggagcc ggtgtgcctg tacgaggggc cggcgcggaa ggctgcagcc
      541 ctctatcccc agaatgtgaa cgtgcaggcg gcgttgagtc tggcgggcat agggatggat
      601 ctaactcggt ccagggtgtt cgctgatccg agcgtgattc agaacaccca cgagatcttg
      661 gccaggggtg ccttcggtga ggttcggatt tccatcagca atattccctc ggacgaatcg
      721 cctaagacag gccgaataac cgcgatgagt gtgacaaagg cgatcaggaa cctgacggct
      781 cccatcgtgg ttgggctgta gtcatagagc agtgaggatg ggcgtggcac ccgcccatcc
      841 tcgtgaggct gtggtgctgg aagcgatcag tgaaagaccc tgccggcgtc cacgaccagc
      901 gtctgcccgg tgatgctgtt cgttctgatg agggtcagaa ccgcgtcggc gacgtcgtcc
      961 ttgtccgcgg ccttcctaag cgcactggat tggcgggccc tctcctggaa ttcgggaggc
     1021 aggttgtccg acatccgcgt accctcaagg aagcccggcg ccacgcagtt gaccagcacc
     1081 cgcggtgcca gggcaacggc catgcaccgc gtgaggtgga tcagcccggc cttggagacg
     1141 gcgtaggcga tgctgcttcc cgacggattc agaccggcga tggaggccac gttgacgatc
     1201 cttccgccgt cctcccccgt catcgccccg gcaattgccc tcatagccag gaacggcgag
     1261 gtcagattgc tgtgcagaat gtggttccac acatcctcgt ccaacgtgtc caggtcctgg
     1321 taggctatga agcggttgta tgcggcgtcg ttgaccagcg cgtcgacgcg accgaacttc
     1381 cggagcgtcg tctggatcat ggtgtcgatc cctgcctggc tggtgatgtc ggcctggatg
     1441 gctatgacct gggggcctag agaacacaat tccgcggcaa atccctcggc ttcggtctgg
     1501 gacttgaggt agacgagcgc cacgttggct cctgccttag ccagtgtcag gcagatgcgc
     1561 cggcccaacc ctcctgttcc tccggtgaca atggccgtct tcccacgaat ctccatcgac
     1621 tattcctctc ttctgacctc gagcacgagc cctatcgttg gacggggcac cccctgttgg
     1681 tgcccgact
//
LOCUS       DATALS010000394         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_400055, whole genome shotgun sequence.
ACCESSION   DATALS010000394 DATALS010000000
VERSION     DATALS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400055"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..355)
                     /locus_tag="VHS06_08820"
     CDS             complement(149..355)
                     /locus_tag="VHS06_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX2988257.1"
                     /translation="MPLVRIDMWPGRDRETKDRMIRNVTKAVCESIGCPPDAVQIMLY
                     EVDKADWARGGVSSAQREAEAKKG"
     gene            614..>1942
                     /locus_tag="VHS06_08825"
     CDS             614..>1942
                     /locus_tag="VHS06_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX2988258.1"
                     /translation="MPHRSDLQADLFGNSMEKPQAEEVLADAPLAARMRPATLDEYVG
                     QEQLIGQGRALRRAIETDRISSFILWGPPGSGKTTLAKIIARATHAHFVGLSAVTAGV
                     ADLRKVIEEAARLQRQQGQRTIVLIDEIHRFNKSQQDSVLHAVEQGVVTLIGATTENP
                     SFEVNSALLSRCRVFTLRALSDDQIEGLMRRAMADPDRGLGKLKVELEPEAMKALVEI
                     AGGDARTALNGLELAATATAPDAQGRRVVSLPAVEDALQRRTLLYDKAGEQHYDVVSA
                     FIKSVRGSDPDAAIYWMARMLEAGEDPLFVMRRMVILAAEDIGLADPQALQVAVAAQQ
                     AVHFIGMPEAWLIMSEAAIYLATAPKSNASYVAIGNARAAVQQRGNMPVPMQLRNAPT
                     SLMKSLGYGKGYDYSHDFAPNDPRRYRQRYLPDGLTGGFYKPSSSGFEAEI"
BASE COUNT          380 a          647 c          584 g          331 t
ORIGIN      
        1 gaacgcgaag tgtgttgggc aacattgcgg cgtcaagccg ccgcactcca gaaggtcacc
       61 cgcgttccca ctcgagtcag cacagcacga ggccgggcac caggttgccc ggcctcgtgt
      121 cttaccatcg cctgatcccg atgggcgact accccttctt cgcctcggct tcccgctgag
      181 cagaggaaac cccacctcgg gcccaatccg ccttgtcgac ctcgtacagc atgatctgga
      241 cggcatctgg agggcatcca atggactcgc acacggcctt cgtgacgttg cgaatcatcc
      301 tgtccttggt ttcccgatcc cggccgggcc acatatcgat acgaactaat ggcatggtag
      361 tacctcccga atctctctca caacgtagtt gggaccgctt gccatccccg gtggaacgtc
      421 ttcgcctcgc ccgtccacac aactccacct ccgagcccgt ggcacgagtc gccagggcaa
      481 gcaggagaaa gttggccgtg gttaacaatt gacgaggtgg tctcgtgccg gtactgtagc
      541 acaaccttgc tcctctctcc accctcacct tacaatgatc tcaggaatca agtagagtcg
      601 gaggaggttc atagtgccac atcggagtga tctgcaggcc gacctgttcg gcaacagcat
      661 ggaaaagccg caagcagagg aggtcctggc agacgcgccc ttggcggcac gaatgcgacc
      721 cgccacgctt gacgagtatg taggccagga gcagctgata ggacaggggc gagcgctgcg
      781 ccgagccatc gagacggacc gtatctcctc cttcatactg tgggggcccc caggctcggg
      841 aaagaccacc ctcgccaaga tcatcgcccg cgccactcat gcccacttcg tcggcctcag
      901 cgcggtcact gccggcgttg cggacctgcg caaggtaatc gaggaggccg cccgcctcca
      961 gcggcaacaa ggccagcgca caatcgtgct gatcgacgag atccaccgct tcaacaaatc
     1021 gcagcaggat agcgtcctgc acgccgtcga gcagggggtc gtcaccctca taggcgcaac
     1081 caccgagaac cccagcttcg aggtgaactc cgcactactc tcgcgctgca gggtcttcac
     1141 cctgcgcgcc ctgagcgacg accagattga ggggttgatg aggagggcga tggccgatcc
     1201 cgaccgtggt ctcgggaagc tgaaggtgga gttggagccg gaagcgatga aggcactggt
     1261 ggagatcgcc ggaggcgatg cccgaaccgc cctgaacggt ctggagctcg ccgcaacggc
     1321 aactgccccc gatgcccagg ggcgacgggt cgtatcgctt cccgccgtgg aagacgcgct
     1381 gcagcgccgg accctacttt acgacaaggc aggcgagcag cactacgacg tcgtctccgc
     1441 cttcatcaag tcggtgcgag gctcggatcc agacgcggcc atctactgga tggcgcggat
     1501 gttggaggct ggggaggatc ctctcttcgt catgcggcgg atggtcatcc tggcggctga
     1561 ggacattggg ctggccgacc cgcaggctct gcaggtcgcg gtggccgctc agcaggcggt
     1621 tcacttcatc ggcatgcccg aggcatggct catcatgtcc gaggcagcaa tctacctggc
     1681 caccgccccg aagagcaacg cctcttacgt ggctatcggc aacgcgaggg ccgcagtgca
     1741 gcagcggggc aacatgcccg tccccatgca actgcgaaac gcccccacca gccttatgaa
     1801 gtccctgggc tacggcaagg gatacgacta ctcccacgac ttcgcaccga acgacccgcg
     1861 gcgctaccgg cagcgctacc tgcccgatgg gctgaccggt ggcttctaca agccctcctc
     1921 ttcgggtttc gaggccgaga tc
//
LOCUS       DATALS010000395         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_178619, whole genome shotgun sequence.
ACCESSION   DATALS010000395 DATALS010000000
VERSION     DATALS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178619"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VHS06_08830"
     CDS             <1..263
                     /locus_tag="VHS06_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEX2988259.1"
                     /translation="AVVEAMSRLAGVPAPKVSAVPRWALHAGGLFSPMLRELDEVLYQ
                     FDRPFVLDSSAFSATFGLLPTPMDEALSATIVWWRDRIRAAA"
     gene            536..1777
                     /locus_tag="VHS06_08835"
     CDS             536..1777
                     /locus_tag="VHS06_08835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2988260.1"
                     /translation="MSRWVELPVNAIAICLVVVIANLGNTIVAPVLPSIRDYFGSSAA
                     DVALMVSGFGFGRLAMDLPAGALTSRISPSKMFAAGILLSGGASALAALSSTLQQLIF
                     FRTIMGIGSAIMTTVALVVLVNSAKPNQRGTVLAFYTSSMLIGGALSPTIGGYLATLF
                     SWRAVFIFCALTPLISFPLNLAVTGHATNKHDSSPTHAKKALEQAASTSRPADSGTAG
                     ANWPALVTIFVSSFLVFFNRQGMQSALLPLYAASELGLDAVNIGNVLSSRAIFTSIVT
                     LPAGALADRIGRKRLLIPGMLFGVVGNLYLLTGESIWIFTASTFLISMTIVGNNMLSA
                     LIADLVPEQWLGRGMGMFRFTCDLGTVLGPFILGLIVDRSGFGAASTLAAGMIMLGVV
                     ACALLVPRHTTPKPSRQTKGV"
     gene            1998..2375
                     /locus_tag="VHS06_08840"
     CDS             1998..2375
                     /locus_tag="VHS06_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2988261.1"
                     /translation="MAHGVTWFEIACSDFDRAKRFYETIFDVTLQDMPVPGQKMAAFP
                     VDWEKGEIGGTIVQREQSKPSSSGTLVYLHGGPDLQTVLDRVVQAGGQVAMPKTPIPM
                     ENAGYMAVFIDTEGNQVGLTSMG"
     gene            2625..>2965
                     /locus_tag="VHS06_08845"
     CDS             2625..>2965
                     /locus_tag="VHS06_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2988262.1"
                     /translation="MAAIPNDQEKELDGRVAIVTGAGQGIGRAAALALAGAGCAVALA
                     ARTATDLDRVAEEIRALGGKALAVPTDVANEGEVETLVRRTLAELGPIDILVNDAGTN
                     TRKPIWEVTTE"
BASE COUNT          567 a          905 c          926 g          567 t
ORIGIN      
        1 gggccgtggt agaggcgatg tcccggctgg ccggggtgcc ggcccccaag gtgagcgcgg
       61 tgccgcgctg ggccctgcac gcaggtggac tcttctcgcc gatgctccgg gaactggacg
      121 aggttctcta ccagttcgac aggcccttcg tgctcgactc gtcggccttc tcggcgacct
      181 tcggcctgtt gccgaccccg atggacgagg cgctcagcgc aacgatcgtc tggtggcgtg
      241 accgaataag agcggcagcc tgatcgtaga cctgagactg cgcggctgta cgggactcga
      301 cttccgtccc aatctgggga cctgggtggg ggagcttgag ggaagggatt gccctggctt
      361 ccccccgatg ggtcgaatag cgatgaagat cgctcagagt agcatgagct cacgtccgac
      421 tttacagcga agaaagcgaa atgctagagt gctgcgcgat ccacatcata ccgcttgtgg
      481 aatgcccgcg gtcacccccg actcgtaacc tcagtcgatt aggaaggtct tgtctttgtc
      541 tcgatgggta gagctacctg ttaacgctat cgcaatttgc ctcgtggtcg tcatcgccaa
      601 cctgggcaac actatagttg cgcccgtgtt gccctccatt cgagactact tcggctccag
      661 cgctgcggac gtggctctga tggtttctgg gttcggtttc gggcggctcg ccatggacct
      721 tccggcgggt gcgctgacca gtcggatcag cccctcgaag atgttcgcag cgggcattct
      781 gctgtcgggc ggagcctccg cgctggccgc cctctcctcc actctccaac agctgatctt
      841 cttccgcacg atcatgggca tcggctccgc aattatgacg accgtagcct tagtagtgtt
      901 ggtcaacagt gccaagccca accaacgagg gactgtgctg gccttctaca ccagttccat
      961 gctgatcggc ggggcgctca gccccaccat cggcggctac ctggccaccc tgttcagctg
     1021 gcgcgcggtc ttcatcttct gcgcccttac ccccctcatc tcctttccgc tgaacctggc
     1081 ggtcaccggc cacgcgacaa acaagcacga ttccagcccc acacatgcca agaaggcact
     1141 cgaacaggcg gcctccacat cgcgccctgc cgatagcggt actgccggcg ccaactggcc
     1201 ggcgctcgtc acgatcttcg tctcctcatt cctggtcttc ttcaaccgcc aggggatgca
     1261 gtcggcactg ctacccctct acgcggcctc agaactcggg ctggacgccg tgaacatcgg
     1321 caatgttctc agctccaggg ccatcttcac cagcatcgtg acactccccg cgggggcttt
     1381 ggcggatcgg atcggcagaa agagactgct gatccccggc atgttattcg gagtggtcgg
     1441 caacctttat ctgctcacgg gcgaaagtat atggatcttc accgccagca cattcttgat
     1501 ctccatgaca atcgtgggca acaacatgct ctctgccctg atagctgatc tcgtacccga
     1561 acagtggttg ggtagaggaa tggggatgtt ccgtttcaca tgtgaccttg gaacggtgct
     1621 ggggcccttt atcctggggc tgattgtgga ccggtccggc tttggcgccg cctcgactct
     1681 ggccgccggc atgatcatgc tgggggtggt cgcctgcgcc ctgctggtcc cccgccacac
     1741 taccccgaag ccgtctcgac aaacaaaagg ggtctaggaa gaggcacggc aaggtataga
     1801 agctgcgttc tacatgatcg atcgtgagga gacgcggctg ttcgaagggg ctgcccataa
     1861 cagccaggag ccgcgcgaga gtggccctcc agaccgtgca gccggcttcg cacgaccgca
     1921 tggaccttcc caccgtggtg ttcagtggaa gtttcccaag aggcgatagg tcgcactcaa
     1981 aacgaaggag atctccgatg gcgcatggag tgacctggtt cgagattgcg tgcagcgact
     2041 tcgaccgcgc gaagcgattc tacgagacaa tcttcgacgt gactcttcag gacatgccgg
     2101 tcccagggca gaagatggcc gcctttcccg tcgattggga gaagggcgag atcggaggaa
     2161 ccatcgtcca aagggagcaa tccaagccga gcagcagtgg gactctggtc tacctgcacg
     2221 gcgggccgga cctccagact gttctggata gggtggtgca ggcgggcgga caggtggcga
     2281 tgccgaagac gccgatcccc atggagaacg cgggctacat ggctgtgttc atcgacaccg
     2341 agggcaacca ggtcggcctc acatccatgg gctgagtcgg gactaaggct tacgagacca
     2401 tagtggagcc ccggggctgt tgctccgggg ctctctgcgt cgaacaggac tccgggttcg
     2461 agtttcgcgt tcttcggagc gccagatagg gaaagtccct tagagcccgc tatgaagctg
     2521 tgactgggtg gggtagactg gtgcaaggcg caaaccctac ccaggcgagg tgaccgacaa
     2581 gaaaggggtc tccatcgccc actcgccgac aaggagtgag gattatggcg gctattccca
     2641 acgatcaaga gaaggaattg gatggccgag tggccatcgt caccggggca ggacagggga
     2701 tcgggcgggc ggcggccctg gcacttgcag gagcaggctg cgcagtggcc ctggctgcca
     2761 gaacggccac agacctcgac agggttgcag aggagatccg cgcgttagga ggaaaggccc
     2821 tcgccgtccc aaccgacgtc gcgaacgagg gtgaggtcga aacactcgtc cggcggacac
     2881 tcgccgagct cggtccaatc gacatactcg tcaacgacgc cgggacgaac acgcgaaagc
     2941 cgatctggga ggtgaccacc gagga
//
LOCUS       DATALS010000396         3283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_400689, whole genome shotgun sequence.
ACCESSION   DATALS010000396 DATALS010000000
VERSION     DATALS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400689"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VHS06_08850"
     CDS             <1..1148
                     /locus_tag="VHS06_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX2988263.1"
                     /translation="LLAGALVGVAWVGSKQRQYAREGIPRLIEWGRVRRTARQIVKEE
                     QETAGWHESWSQYYRGQVERCYPIVTQEMGRDLPAPVDHIVAFTRTEWIDANIANFRE
                     LFEPIEGLYLKLQSPNNLGTLLMGEASQAVLSSQLGVLMGYLSRRVLGQYDLSLLGKE
                     PVTAGRLYFVEPNIAAIQNELALNGDDFRLWIALHETTHAFEFEAYPWVREYFNTLLE
                     EYIQFLSDDLMSFGQGFAGLRGLIERARGNVATGENWIEFMMSPEQRALFAKLQALMS
                     LVEGYSNFIMNAVGERLLPSYQTISERIEQRAARRSPAEKLFVRITGLDLKMEQYRLG
                     ESFIKSVVAAHGLQIANRVWEGPEMVPSLEELRNPRAWVERVVGVAQ"
     gene            1173..1523
                     /locus_tag="VHS06_08855"
     CDS             1173..1523
                     /locus_tag="VHS06_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2988264.1"
                     /translation="MADSRDSEHDEGAFRDPVEPMRYVDAGPHVGFSSPEEFEDRVRL
                     EVHVHANRHTHDHISLSQEEYTPQEVAWLVGSSLEVVMHAIWNGDLKAERRGRDVICI
                     RHEDVTDWLKRRSH"
     gene            1687..2358
                     /locus_tag="VHS06_08860"
     CDS             1687..2358
                     /locus_tag="VHS06_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988265.1"
                     /translation="MRARSTGREREYPEHPLHHVYRRRMRGLRMRWFASGIILGALGG
                     VLLTLIASALVVTQIPQVLQSITGEPDVSVVISESYLNRAASDRIAGGYNLGVPGLNL
                     VALQLGLRPGNLMDLTPSFRVNAGFLSFDISPRVSNQLSVKDGRIAINMIGDPQIGNL
                     NLPLEFLPFNLKDRTTQAVDKVNNDLLISEINQSLTTGFGGSGFDVTSCATENGGLTI
                     RLKHH"
     gene            complement(2377..>3283)
                     /gene="glp"
                     /locus_tag="VHS06_08865"
     CDS             complement(2377..>3283)
                     /gene="glp"
                     /locus_tag="VHS06_08865"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX2988266.1"
                     /translation="ARALPVEVGGPVLVYAGVKPGETVRAAAADVRAGEVVLRAGSSI
                     GPAAIGVLASVGKATVMVHRRPRVAILATGDELVDVERRPGPGEIRNSNNYAVAAQVA
                     SWGAEAFNLGIARDDRRHLVAKLEQALQFKPDLLVTSAGVSVGDYDLVKEVLDAMGTI
                     SMWRVRVRPGKPLAFGRLGEGNVPFLGLPGNPVSAMVTMELFGRPAILKMLGKKRLER
                     PVVMARVLENLENHGGREHYIRGVVSRERDEYVARSTGDQGSNLLTSMAKANALLVVD
                     EKTTLVREGEMVRAMMLEWPDESSR"
BASE COUNT          634 a         1054 c          996 g          599 t
ORIGIN      
        1 gacttctagc gggggccctc gtcggggtag catgggtcgg cagcaaacag cgccagtacg
       61 cgagggaggg catcccaagg ctcatcgaat ggggacgcgt tcgcagaacc gcgcgccaga
      121 tcgtcaaaga agagcaagag acggcgggct ggcacgagtc gtggtcccaa tactacaggg
      181 gccaggtcga gcgctgctat ccgatcgtaa cgcaggaaat ggggcgcgat ttgcccgctc
      241 cggtcgacca tattgtcgcg ttcacgcgca cagagtggat cgacgccaac atcgcgaact
      301 tccgcgaact attcgagccg atcgagggtt tgtacctcaa gctccagagc ccgaacaacc
      361 tcggcacact cctcatggga gaagcaagcc aggccgtgct gagcagccag ctgggcgtgc
      421 tcatggggta cctgtcgagg cgtgtgctcg gccagtacga cctctcactt ctgggcaagg
      481 agcccgtaac cgccggacgt ctctacttcg tagagccgaa catcgcggcc atacagaacg
      541 agcttgcgct caatggcgac gacttccggc tctggattgc ccttcacgag acgacgcacg
      601 ccttcgagtt cgaggcctac ccatgggttc gcgagtactt caatacgctg ctcgaggaat
      661 acatccagtt cttgagtgac gatctaatga gcttcggcca ggggttcgcc gggttgagag
      721 gcctaatcga gcgcgctcgt ggcaacgtgg cgaccggcga gaactggatc gaattcatga
      781 tgagccccga acaacgcgca ctgttcgcca agctccaggc actcatgtcg ctggttgagg
      841 gctacagcaa tttcatcatg aacgcggttg gcgagcgact tctccccagc taccagacga
      901 tcagcgaacg tattgaacag cgagcagcgc gcaggtcccc ggccgaaaag ctcttcgtgc
      961 ggatcaccgg ccttgacctc aaaatggaac agtacaggct cggcgaatcc ttcatcaagt
     1021 cggtcgtcgc cgcgcatggc ctgcaaattg ccaatcgtgt gtgggaaggc cccgagatgg
     1081 taccttccct tgaggagctt cgtaatcctc gagcttgggt cgaacgagta gtaggcgtgg
     1141 cgcaataggc agtgggaatt ggaggataga aagtggcaga ttcccgggac tcggagcatg
     1201 atgagggcgc cttccgagac cctgtggagc cgatgcggta tgttgatgcg ggtccgcacg
     1261 tcggcttctc gagtccggag gaattcgaag accgggtgag gcttgaggtc cacgttcacg
     1321 ccaaccgcca cacgcacgat cacatcagcc tttcccagga ggaatacacc cctcaggagg
     1381 tggcctggct ggtcggaagt agcctggagg tggtgatgca cgccatctgg aacggcgacc
     1441 tgaaagccga gcgccgtggc agagatgtca tctgcattcg gcatgaggat gttactgact
     1501 ggctcaagag gcgatcgcat tagtgagccg ggcgggcgcg atgctcgttt tcgccggccg
     1561 gctgaatagg gaaggcactt gagcgaaccc ccacacccac cggtcgacgg cgaaggaggt
     1621 cacggacggg cctatctctc gccggtctcg ggtcttcggc cggccggggg agaccgtagg
     1681 ggagaagtgc gcgctcggtc gacgggccgc gagcgggagt accccgagca tccccttcac
     1741 catgtctacc gcaggcgaat gcgcggtctg cgtatgcgct ggttcgccag cggaatcatc
     1801 ctgggtgcgc tcggcggcgt tctccttacc ttgatcgcct cggcgctcgt ggttacccag
     1861 atcccccagg ttctacagtc catcaccggc gaacctgacg tctccgttgt gatcagcgag
     1921 agctacctga atcgggcggc ctccgaccgg atagcgggtg gctacaacct cggggtgccc
     1981 gggctcaacc ttgtcgcgct tcagcttggc ctccgtccgg gcaacctgat ggatctgacc
     2041 ccctctttcc gagtgaatgc cggcttcctg tcgttcgaca ttagtcccag ggtgagcaat
     2101 cagctgagcg tgaaggacgg gcgcatagcg atcaacatga ttggcgaccc gcagatcggc
     2161 aatctcaatc ttcccctcga gtttcttcca ttcaacctga aggaccgtac tacccaggcg
     2221 gtagacaagg tcaacaatga cttactgatc tccgagatca accaatcgct caccaccggc
     2281 ttcggcggtt caggcttcga cgtcacgagc tgtgcgaccg agaacggtgg cctcacgatc
     2341 aggctgaagc atcactaacc gcccggaggc gactgttcac cgggacgact cgtcgggcca
     2401 ctcgagcatc attgcgcgca ccatctcccc ctcgcgcacc agcgtagtct tctcatcgac
     2461 gacgagaagc gcattcgcct tcgccatcga ggtcaggaga tttgagccct ggtcgccggt
     2521 gctacgcgcg acgtattcgt ctcgctcgcg gctcacgacg ccgcggatgt aatgttcgcg
     2581 gcctccatgg ttctccaggt tctccaatac ccgggccatg acgaccggtc tctcgagccg
     2641 cttcttcccc agcatcttaa ggatggcggg ccgaccgaat agctccatgg ttaccatcgc
     2701 cgacaccggg ttgcccggca accccaggaa aggcacgttc ccctcgccta gccgtccgaa
     2761 ggcgagcggc ttgcccggcc gcacgcgcac gcgccacatc gagatcgtgc ccatcgcgtc
     2821 gagcacctct ttcacgagat cgtagtcgcc gaccgacact ccggccgacg tcaccagcaa
     2881 gtcgggcttg aactggagcg cctgctcgag cttggcgacc aggtgccggc ggtcgtcgcg
     2941 agcgataccc aggttgaaag cctcagcacc ccaggacgcc acttgcgccg ccacggcgta
     3001 attattcgag ttgcggatct ctccgggccc cggcctccgt tcaacgtcga ccagctcgtc
     3061 accggtcgcg aggattgcca ctcttggccg cctgtgtacc atcacggtcg ccttccccac
     3121 ggaagccagg actccgatcg ccgccggccc aatcgagctc ccggcgcgca gcaccacttc
     3181 gcccgcgcgg acgtcggccg cggccgcccg gacggtctca ccgggtttta ctccggcata
     3241 gaccaacacc ggcccgccca cctcaactgg gagcgcgcgc gcc
//
LOCUS       DATALS010000397         3185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_178679, whole genome shotgun sequence.
ACCESSION   DATALS010000397 DATALS010000000
VERSION     DATALS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178679"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="VHS06_08870"
     CDS             <1..1472
                     /locus_tag="VHS06_08870"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2988267.1"
                     /translation="ATMSVRQRRQRVRFARAAGAPRRKHERRQEERGAADMHRWSPKK
                     GKRVAESLPAPTGKRMSTRASLCALGLRVQHLRLFRALYQSVKISQKKVLYGPVDKLV
                     DCMLGMMCGVAGVCGVNNTVRADVAISLAFGRNGCAEASVIQETLNACTEGNVAQLQG
                     VLKEQLHQHGKCTHHDFKRGLLILDIDFSGQQAGKQAEGSVKCYLAGKRNKRGRQLIW
                     VYASQYDEIVCQWMMPGNTSCNSKGVLQAIVERAQEVLGLTDEQRRRTLIRTDAGFGT
                     MENINWLLEQGYQVLMKAHNQNHVKKVAGTVRQWEESRCRGYEMGWPERPHVYVQPTV
                     QLALRNLKKEEKWECHLLVSTLAALTAAQLKALYNRRGGHPESSFQQDKAGLKLITRQ
                     KKSMQAQQMLIGLGQMAHNILVWARRWPKDAGKESRFERWGIERWVRDLLTIPGFVVF
                     GGQQVRIVLSEAHPLSQGYADAYGGFVQRSGGLLLLGET"
     gene            1537..1734
                     /locus_tag="VHS06_08875"
     CDS             1537..1734
                     /locus_tag="VHS06_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988268.1"
                     /translation="MTQGRKPWAVMSDPLGVEDGAMPVGSGPLRGQTSCRILGSIPTS
                     GDTAPVPYPAGLATSQTGGIP"
     gene            1731..3011
                     /locus_tag="VHS06_08880"
     CDS             1731..3011
                     /locus_tag="VHS06_08880"
                     /inference="COORDINATES: protein motif:HMM:NF021645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HEX2988269.1"
                     /translation="MKRALVGLLLLLAVVPAACGQETHPLPAYVQAPDAAYSWSKASE
                     MTLPFGTATQYSMTSQVWHGITWQHNLWVVKPQKMRANPAVLLLVTGGKGDKPWNEAM
                     MVATLAAQQGIPFAVLFGIPNQPLFDGKKEDALIAYTFVQAIQTGDMTWPLLFPMVKS
                     AVRAMDTVRKVAETEWKTPLKGFVVTGASKRGWTSWLTGAADPRVLGIVPMVYDNLNL
                     QAQMRQQMACYGGYSDQIRDYTELGIQGVMETPRGRELVSAVDPYALRSRLALPKLSI
                     NGTNDRYWTLEAANLYFPGLPGEKHILYIPNAGHGLGDFMDVVNATLAQTRACEEGRK
                     LPTLDWTYREEKDGLRLTVKPGETPVKVAFWTAEAPTCDFRDARWTEQTATGNGFTLS
                     RPQQGYRALFGQVRFRVGDLEFPLSTTPKMIGAK"
BASE COUNT          603 a          937 c         1144 g          501 t
ORIGIN      
        1 gggctaccat gtctgtcaga cagcggaggc agcgggttcg tttcgctcgg gcggcggggg
       61 caccacggcg gaagcacgag agacggcaag aggagcgagg agccgcagat atgcaccgat
      121 ggtcaccgaa gaagggcaag agagtggccg agagcttgcc ggcgcccaca ggaaagcgga
      181 tgagcacacg ggcgagcctg tgtgcgctgg gcctgcgagt tcagcacctg aggttgttca
      241 gggcgctcta ccagtcggtg aagatctcgc agaagaaggt gctctacggc ccggtggaca
      301 aactcgtgga ctgcatgttg ggaatgatgt gtggggtggc cggcgtctgc ggggtgaaca
      361 acaccgtgcg cgcggatgtg gcgattagcc tggcgtttgg gcgcaacggg tgcgcggagg
      421 cgtcggtgat ccaggagacg ctcaacgcct gcacggaagg gaacgtggct cagttacagg
      481 gagtgctcaa ggagcagttg catcagcatg ggaagtgcac gcatcacgac ttcaagaggg
      541 ggttgctgat tcttgacatc gacttctcgg ggcagcaggc gggcaaacag gcagagggct
      601 cggtcaagtg ctacctggcc gggaagcgga acaagcgtgg gcggcaactg atctgggtgt
      661 acgccagcca gtatgatgag atcgtctgcc agtggatgat gccaggcaac accagctgca
      721 actccaaggg agtgctgcag gcaatcgtgg agcgggcaca ggaggtgttg gggctgacgg
      781 atgagcagcg taggaggacg ctcatccgca cggatgccgg atttgggacg atggagaaca
      841 tcaactggct gctggagcag ggctaccagg tgctgatgaa ggcgcacaac cagaaccacg
      901 tgaagaaggt ggctggtacg gtgcggcagt gggaggagag ccggtgccgc gggtatgaga
      961 tgggctggcc ggagaggccg catgtgtatg tgcagcccac cgttcagttg gcactgcgca
     1021 acctgaagaa ggaagagaag tgggagtgcc acctattggt cagcacacta gcggcactca
     1081 ccgcggcgca actgaaggcc ctgtataacc gacgcggagg gcatccggag agcagcttcc
     1141 agcaagacaa ggccgggctg aagctgatca cgcggcagaa gaagagcatg caggcgcagc
     1201 agatgctgat cgggctgggg cagatggcgc acaatatcct ggtatgggca cgccggtggc
     1261 ccaaggatgc gggcaaggag agcaggttcg agcggtgggg aatcgagcgg tgggtgcgcg
     1321 acctgttgac catcccaggg ttcgtggtgt tcggtggtca gcaagtgagg attgtgctgt
     1381 ctgaagcaca tccgttgagt caggggtacg ctgacgccta cggcgggttc gtccagcgaa
     1441 gtggcgggct gctacttttg ggcgaaacat aggtgaaagg gttccacagt tgttcttgtg
     1501 cgaccctttc agggtcgggc gggaggcacg cctcttgtga cccagggtag gaagccctgg
     1561 gctgtgatgt ccgaccccct cggggtcgag gatggcgcga tgccggtggg gtccggcccc
     1621 ctgcgggggc aaacctcatg tcgcatcctg ggctcgatcc ctacgtcggg tgacacggca
     1681 cccgttccct acccggcggg gttggcgacg agccagacag ggggtatacc gtgaaaagag
     1741 ctctagtcgg gctgttgctg ttgctggccg ttgtgcccgc cgcgtgcggg caagagacgc
     1801 atccgctgcc ggcgtacgtg caggcgccgg acgcggccta ctcctggtcg aaggcatcgg
     1861 agatgacgct gcccttcggc acggccaccc agtactccat gacatcccag gtgtggcacg
     1921 gcatcacctg gcagcacaac ctctgggtgg tgaagcctca gaagatgcgt gcgaacccgg
     1981 cggtcctcct gctcgtcacg ggcggcaagg gcgacaagcc ctggaacgag gcgatgatgg
     2041 tcgccaccct ggcggcccag caggggatcc cctttgccgt gctcttcggc atcccgaacc
     2101 agcccctctt cgacggcaag aaggaggacg cgctcatcgc ctacaccttc gtgcaggcca
     2161 ttcagacggg cgacatgacg tggccgctcc tcttcccgat ggtgaagagc gccgtgcgcg
     2221 ccatggatac ggtccggaag gtggccgaga cggagtggaa gaccccgctc aagggcttcg
     2281 tcgtcaccgg cgcatccaag cgcggctgga cctcgtggct gaccggcgcg gcggaccccc
     2341 gggtgctggg catcgtgccg atggtctacg acaatctgaa cctgcaggcg cagatgcgcc
     2401 agcagatggc ctgctacggg ggctacagcg accagatccg cgactacacc gaactgggca
     2461 tccagggcgt gatggagacg cctcgcggca gggaactggt cagcgccgtc gatccctacg
     2521 ccctgcgctc tcgcctcgcc ctgcccaagc tctcgatcaa cggcacgaac gaccgctact
     2581 ggaccctcga ggcggcgaac ctctacttcc ccggcctgcc gggcgagaag cacatcctct
     2641 acatcccgaa cgcgggccac gggctgggag acttcatgga cgtcgtgaac gcgactctcg
     2701 cccagacgcg cgcgtgcgag gaggggagga agctgcccac cctggactgg acctaccgcg
     2761 aggagaagga cggcctgcgc ctcaccgtga agccgggcga gacgccggtg aaggtggcct
     2821 tctggaccgc cgaagcgccg acctgcgact tccgcgatgc ccgctggacg gagcagacgg
     2881 caaccggcaa cggcttcacc ctgtcgcgtc cccagcaggg ctaccgcgcc ctcttcgggc
     2941 aggtgcgctt ccgcgtcggc gacctggagt tccccctctc caccacgcca aagatgatcg
     3001 gggcgaagta gcagtggggt gggcgggccg gtcgagtggc cctcaccccg gccttcggcc
     3061 acccctctcc caagcctcgg agaggggcca cacgccggga cgagttgcgc gaccccagag
     3121 gctggctccc ctctcccatc cgttgggaga ggggtcgcgg gtgagggcct gccggctggc
     3181 gtagg
//
LOCUS       DATALS010000398         9219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_426259, whole genome shotgun sequence.
ACCESSION   DATALS010000398 DATALS010000000
VERSION     DATALS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426259"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(483..1505)
                     /gene="pdxA"
                     /locus_tag="VHS06_08885"
     CDS             complement(483..1505)
                     /gene="pdxA"
                     /locus_tag="VHS06_08885"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX2988270.1"
                     /translation="MTVMANRPIVGVTIGDPAGIGPEIVAKALAEEDLHRAARLVVLG
                     EGKVVRKAVELTGVRLTVHTIESPSQGVFSAGTLDLIDLANLDAESLKPGIPQPQAGQ
                     AGYQFVRKAIEMAMAGEIDAIATAPLNKEALVAGKVPFIDHTAMLGGLTNSPDPMTLF
                     VLDNLRIFFLTRHVSLAEACRQITSDLVLAGLRRADAELKRLGMAHARIAVAGLNPHA
                     GEGGLFGREELEQVAPGVARARAEGIEAFGPIAADSVFFQNRQGKYDAVLSLYHDQGH
                     IAAKTVDFERTVSVTLGLPFIRTSVDHGTAFDIAWKGIASARGMEESIRVAAQYASLF
                     KKATPA"
     gene            complement(1548..2042)
                     /locus_tag="VHS06_08890"
     CDS             complement(1548..2042)
                     /locus_tag="VHS06_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988271.1"
                     /translation="MAYNWAQLAFVEKWRLSAVRGPLVGMYRPADKTYFDLRLHSSEG
                     GARSIPGTVEIHLIDAGDIIREKRIALLETRPVIVRVVTAGATITPSSKEVGSAPPRG
                     RRWLEPGSKITGSVLQTEDDTALVDVGFPVVVQLPEGQGASAFTGHKIEFEVSETPRG
                     FLVV"
     gene            complement(2126..2938)
                     /locus_tag="VHS06_08895"
     CDS             complement(2126..2938)
                     /locus_tag="VHS06_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2988272.1"
                     /translation="MTENNTLYDNWALILGASSGFGAATAKALAKKGLNIFGVHLDRS
                     STLPNAERLMEEIRQTGRQAVFFNTNAANPAKRAEVIDAMAAKLAEQGGQKKVRVLFH
                     SLAFGSLGEFIVDDPKNALNQSKMEMTLDVMANSLVYWTQDVVARGLMGEDGRIYAMT
                     SSGSHRVWPSYGAVSAAKAALESNVRQLAVELAKKKITVNAIRAGVTATPALRAIPGS
                     DKLVEEASAKNPMGRMTRPEDVAAAVGALCVPETYWITGSVYGVDGGEDNIG"
     gene            3320..4438
                     /locus_tag="VHS06_08900"
     CDS             3320..4438
                     /locus_tag="VHS06_08900"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX2988273.1"
                     /translation="MLGYMSLGFWLKWRVLPFPDPIEWFRHSWGNMAYSDLVGIYIRD
                     GLGAHPIPYLQTRLEYPVVAGIFQYLASWAPGVGWYFAALSALLTASALGVFWAITQM
                     SPRSSFWVFAATPPLVLYGTLNWDLLGILFLLMSLLLFEKDRDGWSALFLTLGIWTKL
                     FPAVLLPWMVLTRVREGQWRRLLLFGGVMLSVSVVLNLPFYLGNADGWLYTLRFHANR
                     PPDGGSVWTYLPGISVDWVDRWSLLIGAAGAAGAGIMALTRRWGVAKPALVALACLFL
                     TLKVTSPQYDLWVVPFLALATAPAWLVGLFMGADLLYCWVSFQALYVASGGHGVLGGM
                     QPVALYGANLAHQLALLVVLLWAAGATGWCLHRRHPHP"
     gene            complement(4398..5312)
                     /locus_tag="VHS06_08905"
     CDS             complement(4398..5312)
                     /locus_tag="VHS06_08905"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX2988274.1"
                     /translation="MLRSYSDPNLRSPILLCACSGWPDAASAASGALRYLLLKREARR
                     IAEFDPDTIYKYTTTRPLTVSDGMGGRRLQWPELPWFAMEIPEAKQDLVILLGQEPDL
                     LWRECIQSIGEYAVRLGVSRVVTFGGFLAHVHFAAPPTMTGGSHDLQMRATLRSLGIQ
                     EGDYQGPTGFVTPVLREMADRGIPGASLWIAAPNYLANTSNPRLSAALLHAAEQLLGI
                     DLWVQELDTAGHDMERRIEDALRARPDLASFLRRLSGEAESEDKPESGQEEEAESELP
                     SAEEVLRDLEEHLRRLKGEGDGGAGTTQ"
     gene            5801..6397
                     /locus_tag="VHS06_08910"
     CDS             5801..6397
                     /locus_tag="VHS06_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX2988275.1"
                     /translation="MLDQPDNSEPFANGGLKPRRKDSAATPRRPRPAPPADSGEPKRT
                     ARGKVPIINQKGPSEVDRAIRMAKANSARSVASGRKVAEGTQGRGTVVRIDRVKGFGF
                     LIDAAGEQRFFHRSAVLDSGFANLREQQTVDFEAHNDERGARAVKVKPASSSAAPGKS
                     SPRTPASPKTAKPASGWRSDLMPFRNGTGAPTTGRKKY"
     gene            6481..>9219
                     /locus_tag="VHS06_08915"
     CDS             6481..>9219
                     /locus_tag="VHS06_08915"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX2988276.1"
                     /translation="MNGREFALAALLVLSVPLLAGAGSLRSLGLPWFSGFPAAEGVNP
                     AQLQDGPVDGYLALAPRVMRSGQTESISVSLFNGDAPAAGDVRLSLAGDQGELAASSG
                     RVEGRGEINLMVPQLAEGDYRLQMSGAGFSSESPVRVEEGLLVFLETDKPIYKPGQTL
                     HMRILTLDPQLKPLPGTVNLEVMDAKGLKLFKRDVSTDDFGMASTDLPLSAEPNLGVW
                     KVTAKAGKRSAQLDVRVERYVLPKYEVKLDLARDWALASDPIKGSVSAEYSYGKQVQG
                     EVQLRALRYVGSWQEFANVTRDLDGSAEFQLPAVKYVSGVPSAGGMGNVQLEVTVREK
                     ATGYEEKTTRLLTVAASPLVLKLIPEGVSFKPSLPLSLLVVAETPDRKPQDARVRLNV
                     TYTKSDFSTSQETRDVQVSAGRGMVQITPPADAISLTMSGSGGTGAYTSLAMQSGFSP
                     TGSFITLEQLSKGDLKVGDTARFRINSTKQAANYYYEVLSRGKMVFSDYSRSPEIEFA
                     LTPMMAPTARLLVYQLLPNSEVAADYLPFAVKGDYPQKPQLALARAEARPGDQVGVSL
                     QTDGQARVGLAAVDRSVFILAENRLNLQQLFDELERLYQKPQAELHEARPLDKIGTRG
                     AKEVFGDAGVVVLSNRDVPAGKEYQSPRRFGGPAAAGGVAEGARNMAVPAPMAAAPQA
                     AAAPSAVADASQAGGLMEVQRVRQFFPETWIWDNLTTGQNGRLSKDYTAPDSITTWML
                     RAVALSKQTGLGIAEAQLRVMQPFFVSVDLPYSAIRGEQFPVRVALYNYQNGEEQFQV
                     ELQRDAWFELIDQPVQTVVVGPNDVGSASFTIRPGGLGTNKVKVTARSRSSADAIVKE
                     MIVEPEGVAREQVENLVLSGGSKGQLGVSVPDGVIDGSARAFLSITGSYLTQTIEGL"
BASE COUNT         1597 a         2820 c         2918 g         1884 t
ORIGIN      
        1 ctcccgattg gcgaacttct cgcccaccac gtccatggct gggatcagtg ccccgtccag
       61 cagcgcctgc ggcgcatccc cgccgttcag cgcctgctgg gtcaactcct tcactttggc
      121 ggcgttgccc tcgataacgg cttgcttcaa cgcatcgtag ctgctcattt gacgctccta
      181 tggtgatccc tgattagaga gtacccaaca aggtatagcc ggaccgtagg taatccgaca
      241 gaccgacggc aactttttcc atatcccaac agccttgccg gtcaggtggc aggtgacaga
      301 ctatcttcta atgatataac aaagctgaat aatgacatag cgacagcgta gagccggcac
      361 ggcagtcgcc gaaccgttcg gtcaacgagg ggagattgat ggagttggag gcgtgcgacg
      421 ggggcgtgga ggctgcggcc cgggccatcc aggatcgccg cctccactga cctcagagat
      481 tctcaggccg gagtggcctt cttgaagagg gacgcgtatt gcgccgcgac cctgatcgac
      541 tcctccatcc cccgcgcgct ggctatcccc ttccaggcta tgtcgaaggc ggtgccatgg
      601 tctacggagg tgcggatgaa gggcagaccc agcgttacgc tcaccgtccg ctcgaagtcc
      661 acggtctttg cggcgatgtg gccctggtcg tggtagagcg agagcaccgc gtcgtacttg
      721 ccctggcggt tctggaagaa gacggagtcc gcggcgatag ggccaaaggc ttcgataccc
      781 tcggctctgg cgcgggcgac gccgggcgca acttgttcca actcctccct accgaataga
      841 ccaccttcgc ctgcgtgagg gttgagcccg gcgaccgcga tcctggcgtg ggccatgccc
      901 agtctcttca gttctgcatc tgctcgccga aggcctgcca ggaccaagtc ggaggtgatc
      961 tgcctgcacg cttccgccag cgagacgtgg cgggtgagga agaagatgcg caggttgtcc
     1021 agcacgaaga gcgtcatggg gtccggactg ttggtcagcc cgccgagcat tgccgtgtgg
     1081 tcgatgaagg gcaccttgcc ggccaccagc gcttccttgt tgagcggtgc ggtggctatg
     1141 gcgtctatct cgccggccat ggccatctcg atggctttgc ggacgaattg gtatcctgct
     1201 tgtcccgcct gcggttgagg gatgcccggc ttaagcgact ctgcgtccag gttggcgaga
     1261 tctatcaggt cgagggttcc ggccgagaag actccctggg agggactctc gatggtgtgg
     1321 acggtgaggc ggacgccggt caactcaacc gccttcctga caactttacc ttcgccaagc
     1381 accaccagcc tggccgctcg atgaagatcc tcttctgcca gggctttcgc gacaatctcg
     1441 gggccaatcc cggcagggtc gccgatagtc acgccaacga tggggcggtt agccatcacg
     1501 gtcattacct ccgcgccgcg ctacataatg gcggcgtcac cattcactta caccacaagg
     1561 aaacccctgg gtgtctctga aacctcgaac tcgatcttgt ggccggtgaa tgcgcttgcg
     1621 ccctggccct ccggcaactg gaccacaact ggaaagccta cgtcgaccag cgccgtatcg
     1681 tcttcggtct ggagaacgct ccccgttatc ttggagcccg gttcgagcca acgtcgaccg
     1741 cgcggaggag cactaccgac ctctttggaa ctgggtgtga tcgttgcgcc ggcggtaacc
     1801 accctcacga tgacggggcg agtctccagc agcgcgatgc gcttctcgcg aatgatgtcg
     1861 ccggcatcga tcaggtggat ttccactgta cccgggatcg accttgcgcc gccctcactt
     1921 gagtggagac gcaggtcgaa gtaggtcttg tcggccggac ggtacatccc caccaagggg
     1981 cctcgaacgg cactgagccg ccacttctcc acgaatgcca gttgagccca gttgtaggcc
     2041 atctctacaa cctcacaccg ccgttactgc caccgtccaa taggggcgac gcatgcgtcg
     2101 ccccacggat cacggttatt cgtcgttaac ctatgttgtc ctcgccgccg tccacgccat
     2161 acaccgagcc ggtgatccag taggtctcgg gcacgcagag cgcccctact gccgcggcca
     2221 catcctcagg ccgggtcatt cgccccatcg ggttcttggc gctcgcctcc tctaccaact
     2281 tgtccgatcc cgggatcgct ctgagggcgg gagtcgcggt tacacccgcg cggattgcgt
     2341 tcaccgtaat ctttttcttt gccagctcga ctgccagttg ccgcacgttg gattccagcg
     2401 cagccttggc tgcggatact gcgccgtagc tgggccagac cctgtgagag cccgagctgg
     2461 tcatcgcgta aatccgcccg tcctcgccca tcaacccgcg ggcgacgacg tcctgggtcc
     2521 agtacaccag gctgttggcc atgacgtcca gggtcatctc catcttggac tggttgagag
     2581 cgttcttcgg gtcgtccacg atgaactctc ccagggagcc aaaggcaagg gagtggaaca
     2641 gcactcgcac cttcttttga ccgccctgtt cggccagctt ggcggccatg gcgtcgatta
     2701 cctccgccct cttggccgga ttggcggcgt tggtgttgaa gaatacggcc tggcgccccg
     2761 tctgcctgat ctcttccatc agccgctcgg catttgggag agtggatgat ctgtcaaggt
     2821 gcacgccaaa gatgttcagc cctttcttcg cgagggcttt ggccgtagcg gcaccgaagc
     2881 cgctagacgc accgaggatg agggcccaat tgtcgtatag agtgttgttc tcggtcacca
     2941 ccgtcacctt cctgcccacg gctgactaac cggcggtcgg atgaatccga ccgccgtgga
     3001 agagtcgcga gagacataga tactatctgg atgcattcag cgaactttac ggtatggggt
     3061 gtggcgtgtc aaccggcgag ggcggtatcc tgtatggttc gccgacaggg tggccgcatc
     3121 gcgtggcgca gcctgtagct gcctgtcgtt acgggatgaa gacccatcca gaaaagaagt
     3181 agaaggtggt accttggcat tccgcttgct cgcctcgttc cacagtagtg acgttaggag
     3241 ttctgacatc gagacgcatc tggccccaag cgcccggccc cccttctcgt ggaggcgacg
     3301 gggaggttgg gctctgctcg tgctgggata catgagcctc ggcttctggc ttaagtggag
     3361 ggttctgccg tttccggatc ccatcgagtg gtttcggcac tcctggggga acatggcgta
     3421 cagcgacttg gtggggatct acattcgcga cgggttgggg gctcacccta tcccctacct
     3481 tcagacgagg ttggaatatc cggtggtggc cggcatcttc cagtacttgg cctcctgggc
     3541 tccgggtgtg gggtggtact tcgccgcttt gtcggctctg ctgactgcct cggcattggg
     3601 agtcttctgg gccatcactc agatgtctcc gcgatcttcg ttctgggtct ttgcggccac
     3661 tccgcctctt gtgctgtacg gaacgctgaa ctgggacctg ctggggatcc tcttcctgct
     3721 gatgtcactg ctgctcttcg agaaagaccg cgacggctgg agtgctctct ttctcacact
     3781 cggaatttgg accaagctgt tccctgccgt gctgctgcca tggatggttc tgactcgtgt
     3841 gcgggagggg caatggcgtc ggttactcct cttcggtggc gtgatgctgt cggtctccgt
     3901 agtgctaaac ctccccttct acctgggcaa tgcggacggc tggctctaca cgttgcgctt
     3961 tcatgcgaat cgacccccgg acggtgggtc ggtctggaca tatctgcccg gcatctcggt
     4021 tgactgggtg gatcgttgga gcctactgat aggggctgcg ggtgcggccg gcgcggggat
     4081 tatggctctt acccgtcgat ggggtgtcgc caagcctgcg ctggtggcgc tggcctgtct
     4141 gttcctcacc ctcaaggtta cgagtccaca gtacgacctc tgggtcgtgc ccttcctggc
     4201 gttggccacg gccccggcat ggctggtggg attgttcatg ggggcggacc tgctctactg
     4261 ctgggtgagc ttccaggcgt tatacgtagc ctcaggcggg cacggggtgc tgggggggat
     4321 gcaaccggtg gcgctctacg gtgcgaactt ggcccaccaa ttggcgctgc tcgttgtgct
     4381 gctgtgggcc gcgggggcta ctgggtggtg cctgcaccgc cgtcaccctc acccttgagt
     4441 cggcgcagat gctcttccag gtcgcgcagt acctcttccg cggaagggag ttcggactcg
     4501 gcctcctcct cttgtccact ctccggtttg tcctcgctct ccgcttcacc gctgaggcga
     4561 cggaggaagc ttgccaggtc tggacgagct ctcagagcat cttcgatgcg ccgttccatg
     4621 tcgtgtccgg cagtgtccag ttcctggacc cagagatcta tccccagcag ttgctccgct
     4681 gcgtgcagga gcgccgcgga gagtctcggg tttgaggtgt tggccaggta gttgggcgcc
     4741 gctatccata gggaggctcc cggtatcccc ctgtctgcca tttccctgag gactggcgta
     4801 acgaagccgg ttggcccctg atagtcacct tcctggatcc ccaggctcct aagggttgcc
     4861 ctcatctgca gatcgtggct gccccccgtc atcgtggggg gagcggcgaa atgtacgtgg
     4921 gcgaggaatc ccccgaaggt gacgacccga gagacaccga gccgaaccgc gtactcgccg
     4981 atggactgga tgcattccct ccagagcagg tccggctcct gcccaagcag gatgacgagg
     5041 tcctgtttcg cctcgggaat ctccatcgcg aaccacggca gttccggcca ctggagcctg
     5101 cgtcctccca tgccatcgga gacggtgagg ggccgagtgg tggtgtactt gtagatggta
     5161 tccgggtcga actccgcgat ccgtcttgcc tctcgcttta gcaggaggta gcgtagagcc
     5221 ccactggcgg cagaggctgc atccggccaa ccgctgcagg cacacagcag gatgggcgat
     5281 ctcaagtttg gatcggaata gcttctcagc atgctcacat tatgctacgc gggcctcttt
     5341 ggatcaaatg aaaacggcag ttgagcagct atcgacacgc cccgcgcgat gtgatacact
     5401 tactgtggct cggtttcagg gctacgccga cgacggcgtg gcacctcatt gggttgttcc
     5461 ccgttgaagc ctactccaac cggcccctgc gctccatggg actggtgagg gtcgatttgg
     5521 tcgatcttgg ctcgtctcct tcgcttgttc tgagacgacc acacaaggct ttgagaccta
     5581 ccccgaagcg agaacggatt gccccgcgac aggtatcgtc ggccaataga tcgggcgtgc
     5641 tgctaacgag gctcgcgtga tcgctccgta gtgtggttcg accgttggat cgctctcgat
     5701 tcttggtttc ctcggtttga gagtcgatct atcactgctg gattgtggct cgtactatgc
     5761 catgttcgtt gatgagagta tctctaggaa aggacctcta gtgctagacc aacctgacaa
     5821 ctccgaaccg tttgcgaacg gcggtctgaa gcctcgtcgg aaggattccg ccgccacgcc
     5881 tcggcgccct cgacctgcac ctccagccga ctccggcgaa ccgaagcgca cagccagggg
     5941 aaaggtcccc atcatcaacc agaagggacc gtctgaggtg gaccgtgcca tccgcatggc
     6001 aaaggcgaac agcgccaggt cggtagcctc agggcgaaaa gtcgccgagg gcacgcaggg
     6061 gcgtggcacc gtcgtccgca tcgatcgcgt caagggcttc ggcttcctga tcgacgccgc
     6121 gggtgagcag cgcttcttcc atcgctccgc ggtgctggat tcgggtttcg ccaacctcag
     6181 ggaacagcag acggtagact tcgaggcgca caacgacgag cggggggctc gtgcggtgaa
     6241 ggtcaaaccc gccagttcgt ccgctgcgcc cggaaaatcc tcgccgcgta ctcccgcctc
     6301 acctaagacc gccaagccag caagcgggtg gcgatcggat ctgatgccat tccgcaacgg
     6361 aaccggcgct cccactactg gacggaagaa gtactagctt ccgacgcaag agcaccgcgg
     6421 gaactttttg gttgcctcgt tcgttatcca cgtagaacac ccaatactgg gaggctacgg
     6481 atgaacggac gtgaatttgc cctggccgcc ttgctggttc tctcggttcc tctgttggct
     6541 ggcgccggca gtctgcgcag ccttggtcta ccctggttct ctggcttccc cgcggctgaa
     6601 ggagtgaatc cggcacagct gcaggatggg ccggtagatg gctacctggc ccttgcaccc
     6661 agggtgatgc gctccggtca gaccgagtcg atctccgttt cgctcttcaa cggtgacgct
     6721 ccggccgccg gtgacgttcg gctctcccta gcaggggatc agggggagtt ggcggcctca
     6781 tcagggcgtg tagagggccg cggcgagatc aacctcatgg tcccgcagct tgccgaaggg
     6841 gactaccggc tccagatgag cggagccggc ttcagttccg agtctccagt tcgggtcgag
     6901 gaggggctgt tggtcttcct agagaccgat aagccgatct acaagcccgg gcagaccctg
     6961 cacatgcgca tcctcaccct cgatccccag ctgaagccgt tgcctggtac cgtgaacctc
     7021 gaggtgatgg acgccaaggg actgaagctg ttcaagcggg atgtctccac cgacgacttc
     7081 gggatggcct ccaccgacct ccctctttct gcggaaccta acctgggagt gtggaaggtg
     7141 accgcgaagg cggggaagcg ctcggcccaa ctcgacgtaa gggtggagcg ctatgtactg
     7201 cccaagtacg aggttaagct ggatttggcc agggattggg ctttggcctc cgacccaatc
     7261 aaaggcagcg tgagtgcgga gtattcctac ggaaagcagg tgcagggcga ggttcagctg
     7321 cgcgcgctgc gctacgtggg aagctggcag gagtttgcca acgtcacgcg agacctcgac
     7381 ggtagtgcgg agttccagtt gcctgccgtc aagtacgtca gcggggtccc atcggccggc
     7441 ggcatgggca atgtgcagtt ggaagtcact gttcgggaga aagccaccgg gtacgaggag
     7501 aagaccacgc ggctgcttac cgtcgctgcc tcccctctgg tgctgaagct gatccccgag
     7561 ggggtgagct tcaagccctc gctgcccctc tccctcctgg tcgtggcgga aacgcccgac
     7621 cggaaacctc aggatgccag agttcggctg aacgtcacct ataccaagtc ggacttcagc
     7681 accagccagg agactaggga tgtgcaggta agtgccggcc gtggcatggt tcagatcacc
     7741 cctcccgccg acgccatttc gctgaccatg agtggatccg gcgggaccgg cgcctatacg
     7801 tcgctggcta tgcagtcggg tttctccccc accggcagct tcatcacgct ggaacagttg
     7861 agcaaaggcg acctgaaggt gggtgacacc gcccgcttcc ggatcaactc cacgaagcag
     7921 gcggcgaact actactacga ggtcctctcc cgcgggaaga tggtcttctc cgactactcc
     7981 cgtagccccg agatcgagtt tgccctcacg ccgatgatgg ccccaaccgc tcggctgctg
     8041 gtgtaccaac tgttgcctaa ctcggaggtg gccgcggatt acctgccctt cgccgtgaag
     8101 ggcgactatc cccagaagcc gcagttggcg ctggccaggg ccgaggcgcg acccggcgac
     8161 caggttgggg tgagcctgca gaccgatggc caagcccgag tggggttggc ggcggtggat
     8221 cggtcggtct tcattcttgc ggagaaccgg ctcaatctgc agcagctgtt cgatgaactg
     8281 gagcggttgt accagaagcc gcaggctgag cttcacgagg ctcgccccct ggacaagatc
     8341 ggaacccgag gggcgaaaga ggtgttcggg gatgccggag tggtggtgct gagcaacagg
     8401 gatgtgccgg caggcaagga gtaccagtcg cctcgcaggt tcgggggacc ggcagctgct
     8461 ggaggggttg ccgagggagc gaggaacatg gcggttcccg cgcccatggc tgcggccccg
     8521 caggctgcgg cggctccgtc cgctgttgcc gacgcttcgc aggccggtgg gctgatggag
     8581 gtgcagcgag ttcggcaatt cttccccgag acctggatct gggacaatct gacgaccggc
     8641 cagaacggca ggctgtccaa ggactacacg gcgccggata gcatcactac ctggatgctc
     8701 cgggcggtgg cgctctccaa acagacgggt ctggggatcg ccgaggccca gctgagggta
     8761 atgcagccct tcttcgtctc ggtggatctg ccctactcgg ccatccgagg cgagcaattc
     8821 ccggttaggg tcgcgctgta caactaccag aacggcgagg agcagttcca ggtggagttg
     8881 cagcgagacg cctggttcga gttgatcgac cagccggtgc agaccgtcgt cgtcggtccc
     8941 aacgacgtcg gatcggcttc cttcaccatc agaccgggtg ggttgggaac caacaaggtc
     9001 aaagtgacgg cacggtctcg atcatccgcg gatgccatag tgaaggagat gatcgtcgag
     9061 ccggagggag tcgctcggga gcaggtggag aacctggttc tctcgggtgg atcaaagggg
     9121 cagttgggcg tgtcggtgcc cgacggggtc atcgacggct cggcccgcgc cttcctctcc
     9181 atcacgggga gctatctcac ccagaccatc gaggggctg
//
LOCUS       DATALS010000399         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_44711,
            whole genome shotgun sequence.
ACCESSION   DATALS010000399 DATALS010000000
VERSION     DATALS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44711"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VHS06_08920"
     CDS             complement(<1..259)
                     /locus_tag="VHS06_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988277.1"
                     /translation="MDGDVPVTGNRIEIQDLPLWEKMAAKRAPVSFDLEVTGRCNNNC
                     RHCYINLPAGDSAAENAELGTRELLGIARQATDLGAVWCLLT"
     gene            365..2218
                     /locus_tag="VHS06_08925"
     CDS             365..2218
                     /locus_tag="VHS06_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988278.1"
                     /translation="MASLEGVPKEGLLAEKKHRLNARDGLRLLRALKLVWSVAPRYTV
                     ASVGLTVLQGLLPLAGVWLMKLIIDGVTSGVTGPDRGHTFGRVAILIALAAGVGLAGV
                     VLRSLAAMVDQALGQTVTDHVTDLIHAQSVAVDLEYYENPRYHDALHRAQQEAPFRPT
                     SIVRNLTNTGQAVVSLVAMAGLLLTLHWAVGLIVLGAAIPGAVVRFRYAGKLFAWQRR
                     RTEAERESYYAHWLLTGDAHAKEIRLFGLGDRFRRWHRDLRGDLRRELIGLTGRRSLA
                     ELGAGAGAVVAVFGTFAFIAWRAIQGAITLGMMVAYYQAFQISLTSLQAVLGGVALLY
                     EDSLFLTGYDEFMSLEPRIQAPARPAPVPQHMAQGIRFESVDFRYPDTERTALKDVSL
                     SILPGEVTALIGPNGSGKSTLVKLLCRLYDPVDGVITLDGIDLRRFDTVDLRRHMSVV
                     FQDFARYQVTAGENIRLGNVWLPERDPAIESAARSAGIHEALAGLQRGYDTLLGKWFD
                     GGEELSVGEWQKVALARAFVRDAQILVFDEPTSGLDPRAEREVFQHIKLLAQDRAVIL
                     VSHRFSTVRAADHIHVFDRGSVVESGAHEELMALDGRYASMYRIQTGTSPA"
     gene            2469..>3041
                     /locus_tag="VHS06_08930"
     CDS             2469..>3041
                     /locus_tag="VHS06_08930"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEX2988279.1"
                     /translation="MKAYAYSDYLWLPLLLAVFVAFLASYGWRRRTVQGALPFFIGCL
                     FAVAWCIGSLLETAAVDPNTKIFWLRFLTLWQLPIVSAATCFALQYSGLDRWLTRRNV
                     ALLAAPPVVFMAFILTDGLHHLVWTGFTFDGAHVTGIRGPVLLVFLTYSYLLFFVNIC
                     ILSWLFITSPRHRAPAALMVVGQISARIVFE"
BASE COUNT          463 a         1004 c          958 g          616 t
ORIGIN      
        1 cggtcaacag gcaccagacg gcgcccaggt cggtcgcctg gcgcgcgatg cccagaagct
       61 cccgggtgcc gagctccgca ttctccgccg cactgtctcc agcagggagg ttgatgtagc
      121 agtggcggca gttgttgttg cagcggccgg ttacctccag gtcgaatgac accggggccc
      181 gcttggccgc catcttctcc cacagaggga gatcctggat ctctatccgg ttgccggtca
      241 ctggcacgtc gccatccaag cccgctttcc ttgatcggca accgccgcgc tctcgtcgtg
      301 gcacgggtcg cgtgcgccac gatgttaaca cctggatttt gtctatagtt ggtgtctcac
      361 cgacgtggcg tctttggaag gcgtgccgaa ggaggggctg ctggccgaga agaaacacag
      421 gctgaacgct cgggatggtc ttcgcctgct ccgcgcgctg aagttggtct ggtccgtcgc
      481 cccgcgttac acggtggcga gcgtggggct caccgtgctg cagggcctgc tcccgctggc
      541 cggggtctgg ctcatgaagc tcatcatcga tggggtcacg agcggcgtga ccgggcccga
      601 tcgcggacac accttcgggc gggttgccat cctcatcgcc ttggcggccg gggtcggcct
      661 cgcgggggtc gtcctacgct ccttggcggc catggtcgac caggccctgg gacagacggt
      721 gacggaccac gtcacggatc tcatccacgc ccagtcagtc gcggtcgacc tcgagtacta
      781 cgagaacccc cgctaccacg acgctctgca ccgggcccaa caggaggcgc ccttccggcc
      841 cacgagcatc gtcaggaacc tcaccaacac cgggcaagcg gtcgtttcgc tggtggcgat
      901 ggcaggtctc ttgttgaccc tccattgggc cgtgggcctg atcgtcctcg gcgcggccat
      961 cccgggagcg gtggtgcgct ttcgctatgc gggcaagctc ttcgcgtggc aacgccgccg
     1021 cacggaggct gagcgcgagt cctactatgc ccactggctg ctgacgggtg acgcccacgc
     1081 caaggagatt cgcctgttcg ggctgggaga tcgcttccgc cggtggcacc gcgatctgcg
     1141 tggggatctg cgccgcgagc tcatcggact caccggccgg cgctctctgg cggaactggg
     1201 agcgggtgcc ggcgccgtag tggccgtgtt cggcactttc gcattcatcg catggcgcgc
     1261 catccaggga gccatcaccc tgggaatgat ggtggcctac taccaggcct tccagatcag
     1321 cctcacctcg ctgcaggctg tgctcggcgg tgttgccctc ctctacgagg acagcctttt
     1381 cctcaccggc tacgacgagt tcatgtctct cgaaccccgc atccaggccc cggctcggcc
     1441 tgcaccggtg ccccaacaca tggcgcaggg catccgcttc gagagtgtgg atttccgtta
     1501 tccggacacg gagcgcaccg ctctcaaaga cgtctcgctg tccatcctac cgggcgaggt
     1561 gacggctctg atcgggccca acggatcggg gaagtccacg ttggtcaagc tgctctgccg
     1621 cctctatgac cctgtcgacg gcgtgatcac cctcgacggg atcgacctgc gccggttcga
     1681 caccgtggac ctgcggcggc acatgagcgt ggtcttccag gacttcgccc ggtaccaggt
     1741 gacggccggc gagaacatcc ggctgggtaa cgtctggctg cccgagcgcg accccgcgat
     1801 cgaatccgcg gcccggagcg ccggcatcca cgaggccctg gcgggtctcc agcgcgggta
     1861 cgacacactg ctggggaagt ggttcgacgg gggcgaagag ctaagcgtgg gtgagtggca
     1921 gaaggtggcg ctggcgcgtg ccttcgtgcg cgatgcccag atacttgtct tcgacgagcc
     1981 gacgagcggc ctcgatccgc gcgccgagcg cgaagtgttc cagcacataa agctgctggc
     2041 ccaggaccgg gccgtcatcc tggtcagcca ccgcttctcc acggtgcggg ccgcggacca
     2101 catccacgtc ttcgatcgcg ggagcgtcgt ggaaagcggc gcccacgaag aactcatggc
     2161 gctggacgga cgctacgcct cgatgtatcg gatccagacc gggacctcgc ccgcgtgagg
     2221 cgcggaaaca aataccccgt tgggcgcagg cacgagagcc gcttcagact cgttgcggcc
     2281 gctattctcc agactttcca gacaaatatc tgctacacct cggcctagcg ggctgagatg
     2341 atgttgaagt tgtttgcccc cgactccctc cccatgtggc ccagtttgct ttcaaggggt
     2401 gagcgaggag cctatcgttc gggtccactc cttcatgttt gtccggactt cagttaaagt
     2461 cggacggaat gaaggcctat gcctacagcg actatctgtg gttaccgtta ctcttggccg
     2521 tcttcgtcgc ctttcttgcg tcttatggct ggcgtcgtcg cactgtgcag ggcgctttgc
     2581 ccttcttcat cgggtgtctg ttcgcggtcg cttggtgcat agggtcgctg cttgagactg
     2641 ccgcggtcga tcccaacacc aagatcttct ggctgaggtt cctgaccctg tggcaattgc
     2701 ccatcgtaag cgctgccacc tgtttcgcac tgcaatactc gggcttggac cggtggttga
     2761 cgcgccgcaa tgtggcgctg ctggcagcac ctccggttgt cttcatggcc ttcatcctga
     2821 ccgacggcct tcaccacctc gtctggaccg gtttcacctt cgatggtgca cacgtcaccg
     2881 gcattcgtgg ccccgtcctc ctggtcttcc tgacctacag ctacctgctg ttcttcgtga
     2941 acatttgcat cctcagctgg ctgttcatta cctctcccag gcaccgggct ccggccgctc
     3001 tgatggtggt cgggcagatc agcgcccgca tcgtctttga g
//
LOCUS       DATALS010000400         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_269545, whole genome shotgun sequence.
ACCESSION   DATALS010000400 DATALS010000000
VERSION     DATALS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269545"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="VHS06_08935"
     CDS             complement(<1..137)
                     /locus_tag="VHS06_08935"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX2988280.1"
                     /translation="MSDEFRLTLTYDDVLLEPKHSSIASRRNVDTSTRLSRNITMAVP
                     IV"
     gene            291..1592
                     /locus_tag="VHS06_08940"
     CDS             291..1592
                     /locus_tag="VHS06_08940"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX2988281.1"
                     /translation="MNDIRPFKGLRYNESAAGPLGKLISPPYDVISNLEQLDLHGRST
                     HNVVRLEFGFQYPEDDKSDSRYTRAAALLQQWISEGVLRPEDDESIYLYEQQFTYEGA
                     TLTRRGVLAALRLAPWEEGVVLPHEETLAGPKVDRLELMRATACNLSPLYLLFDDPSG
                     GARREMESITRRAPDAMADAGDGQLHRLWVLPGHDHAALLSALRQPQLYMADGHHRYE
                     TALAYMAERRAADPQGSPDAPYNFALVLLVDATDPGLVVLPTHRLIHDVDPKVLAGLD
                     AALSASFEAEEMLVIDSSKTEANAATALRVLREAEERGPALALYRAGSITLLRPRQSP
                     AAPASGRPLLDVDILHEGILGPMLGIDAERLKAGTNVAYTRDAVEALSAVDQETAQAA
                     FLMNPTRVEQVLETARAKGKMPQKSTYFYPKPATGLVIKQL"
     gene            complement(1642..2268)
                     /locus_tag="VHS06_08945"
     CDS             complement(1642..2268)
                     /locus_tag="VHS06_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988282.1"
                     /translation="MSTELGRIEKPAAEQFVAERKIYLVPLVFSPSQPTKEYADIYER
                     YWSGVREHLLKLEGRIGSVVKVYHESVGFAGEDGLKAAEELSDKSGALARSKVDAGAV
                     FEALEDPELVSESFDWQRCLMIGLESRAAAEKVVGFYSDITKRRYELMSKRIDETLKP
                     GEAGLLFISEDHRVQFPTDVRVFFVSPPALDEVHRWLRDQRTNPPTEQ"
     gene            2448..2696
                     /locus_tag="VHS06_08950"
     CDS             2448..2696
                     /locus_tag="VHS06_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988283.1"
                     /translation="MKDIVQIIFVGLLVLALVLVGPIGGILLTAASKAVGIQPGAVFV
                     LAAVALPAAFAIIRSADGRRTPGRLSEAAAASRNRKAA"
BASE COUNT          587 a          922 c          941 g          648 t
ORIGIN      
        1 actatcggca cagccatggt tatgtttcgg ctgagccggg tcgaggtgtc cacgtttctg
       61 cgtgacgcga tggatgagtg cttcggctcc agcagaacgt cgtcataggt gagtgtaagc
      121 ctgaactcgt cgctcatctt ctcctacctc caaccgtccg gccggacacc gtcttgtaca
      181 ctccaccgga cgccgctgat tatacatgca cgtaccgcgc cgaaggccgt ttgatggttg
      241 cccgcagcga atggtaccct tgtagtagca atcagctagc ggaggctacg gtgaacgaca
      301 ttcgtccttt caaggggttg cgctacaacg agtctgcggc tggtccgttg ggaaagttga
      361 tcagccctcc ctacgacgtt atctccaacc tggagcagtt ggaccttcac ggccgaagta
      421 cccacaatgt ggttcggctt gagttcggtt ttcagtaccc cgaggacgac aagtccgaca
      481 gtcgctacac cagggccgct gccctgctgc agcagtggat ttcggaaggg gtactgcgcc
      541 cggaggatga tgagtcgatc tacctgtatg agcaacagtt cacctatgag ggagctaccc
      601 tgaccaggcg cggagtcctg gctgctctgc gattggcacc gtgggaagag ggcgtggtgc
      661 tgcctcacga ggagaccctg gcggggccca aggtcgatcg tctggagttg atgagggcta
      721 cggcctgcaa tctcagcccg ctctatctgc tgttcgatga cccgtctggc ggagcaaggc
      781 gtgagatgga gagcatcacc aggagggcgc cggacgcgat ggcggacgcc ggcgacggcc
      841 agcttcaccg actgtgggtg ttgcccggac acgaccatgc cgcgctcctc tccgccctca
      901 ggcagccgca gctttatatg gccgatggcc atcaccggta cgagacggcg ttggcctaca
      961 tggcggagag gcgtgcggcc gacccccagg gtagcccgga cgcaccgtac aattttgcgc
     1021 tggtcctgct ggtggatgct accgatcccg ggctggtggt ccttcccacc catcggctga
     1081 tacacgatgt cgatccgaag gtgctggcgg ggctggatgc ggcgctctct gcctcgtttg
     1141 aggcagagga aatgctagtg atcgacagct ccaaaaccga agctaacgct gcaactgcac
     1201 ttcgggtgct gcgggaggcc gaggagcgag gacctgccct ggcactctat cgcgccggat
     1261 ccatcactct attgcggccc cgacagtcac cggccgctcc cgcttccggc aggcccctcc
     1321 tggatgtgga tatcctgcac gagggcatcc tcgggccgat gctggggata gatgctgagc
     1381 gtctgaaggc cggcacaaat gtagcctaca ccagggatgc cgtggaggcg ctgtccgcgg
     1441 tggatcagga gactgcacag gctgccttcc tgatgaatcc gactcgggtg gagcaggtgc
     1501 tggagacggc gcgggctaaa ggcaagatgc cacagaagtc cacctacttt taccccaagc
     1561 ctgccacggg tctggtgatc aagcaactct gagcaatcgg caaaatgggt aggggcgaac
     1621 cagtgtgtcc gcccctacca gctattgctc cgtaggcggg tttgtcctct ggtctcgcag
     1681 ccagcgatga acctcgtcca gcgccggtgg ggagacgaag aacaccctga cgtccgtggg
     1741 aaactggacg cggtgatcct cggagatgaa tagcagtcct gcctcccccg gcttcagtgt
     1801 ctcgtcgata cgcttggaca tcagttcgta ccgtctcttg gtgatgtcag agtagaagcc
     1861 cacgaccttt tcggccgccg cgcgactctc cagcccgatc atcagacagc gctgccagtc
     1921 gaagctctcg gagaccagtt ccggatcctc tagcgcttcg aagacggcgc ccgcatccac
     1981 cttgctgcgc gcaagggcgc cgctcttgtc gctgagttcc tcggctgctt tgagcccgtc
     2041 ttccccggcg aatcccaccg actcgtggta gaccttcacc acggagccga tgcgcccctc
     2101 cagtttcaac aggtgttccc taaccccgga ccagtagcgc tcgtagatgt ccgcgtactc
     2161 cttggtcggc tgcgaagggg agaatactag cggcacgagg tagatcttgc gctcggcgac
     2221 gaactgctcg gcggctggtt tctcgattcg tcccaattcg gtagacatgg cgttcctccc
     2281 tgccagtagc gctgacggtt acaggatagc cacctgcccc tgtcctgtct atatcaacga
     2341 ggttatggaa ttgccgtggg gttggcattg gacatcatga tcatttgaga gcattattgc
     2401 cgtgaaatta aagtaaatgt tgtgacagac cgaggaggaa ttcaaagatg aaggacatag
     2461 tccagatcat atttgtgggg cttttggtgt tggccctcgt tctcgttggt cccatcggtg
     2521 ggatactgct gacagcggcg tccaaagccg tggggatcca gcccggtgcc gtgtttgttc
     2581 tcgctgctgt agctctacca gcggcattcg caatcattcg atccgctgac ggccggcgca
     2641 ctccaggtcg actctctgag gccgccgcgg cttcgcgcaa ccggaaagct gcatagactc
     2701 cctccctgca gaaggagggg gcggggagac ctgctccctc cttctgcgtt ctttctccca
     2761 ccccatttcg cgctgttttc ctctcgacct tcctgctgag accgcctttc gcatggccca
     2821 cccgctacta atgtaaataa cgtcatggca aaagcgagcc ggggcgctca agagaagcct
     2881 gacttttgcc gattattact agtgaatagg tagaaccttg gcagttgagg cgatccatac
     2941 aagaggtgcc ccatggaaga aaagctgcgg agcgaacagg gcgatacccc cggcaggcag
     3001 agtggtgccg gtattgttgc aggtggactg ggcaaatcca agccgttgat gggcgagaag
     3061 tcggctgcct ctactgcccc gcaccaggat gctgtggc
//
LOCUS       DATALS010000401         6307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_336556, whole genome shotgun sequence.
ACCESSION   DATALS010000401 DATALS010000000
VERSION     DATALS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336556"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VHS06_08955"
     CDS             complement(<1..644)
                     /locus_tag="VHS06_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2988284.1"
                     /translation="MSEHIFIGVAWPYANGPLHLGHLAGALLPPDIFARFNRVKGNQV
                     LMVSGSDEHGTPITVRAEREGKSPKDIVDFYHEQFSENLKAIGVSYDLFTRTTTENHY
                     QVVQDLFLRLLENGYIDKQEMFSPFCPSEQRFLPDRYVEGTCPFCNYPEARGDQCDNC
                     GKPLDPTDLINPRCKFDGTTPEIRATEHFFLKLSKLNQALIEWVEPKTYWRPNV"
     gene            complement(674..1438)
                     /locus_tag="VHS06_08960"
     CDS             complement(674..1438)
                     /locus_tag="VHS06_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEX2988285.1"
                     /translation="MDRPYLSLVVPAYNEEGRLPATIEKLRAFLKRPPFPYEVLVVDD
                     GSTDGTAALVEKSIPSLPELRLLKEPHRGKGHAVRQGMLAARGQYIMFCDADFSMPVE
                     ETPNFPTAISESCQVAIGSREVKGARRIGEPRYRHLMGRVFNLLVRVLTMPDLQDTQC
                     GFKCFSRKAAKDIFGRQVIDGFGFDVEVLYLARKLVYGITEVPVSWYYSPSSRVDPVR
                     DTLRMIGDVLRVRSNDRNGLYDTPPQPRENPETVID"
     gene            complement(1438..2427)
                     /locus_tag="VHS06_08965"
     CDS             complement(1438..2427)
                     /locus_tag="VHS06_08965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2988286.1"
                     /translation="MPDQLAETAPYISVVIPNWNGARYLAGCLDSLRAQSFRDFEVLV
                     VDNGSTDGSDRMVQERYPEFRLVPLPVNQGFTVAVNVGIRESRGEIVALLNNDAEARE
                     DWLETLAGAGRSHPESDFFACKILLFDQRDTIHSAADFYSTDGLPGNRGVWQKDTGQY
                     DREEFVLLACGAASGWRKSLFEKVGLLDESLFMYCEDVDLGLRAQLMGHRCLYLPQSV
                     VYHRLSATGGGPLASYYCGRNLITVAVKNLPSSILRRSWPSIVRSQAVFAVNSICHWR
                     EKAARARLRGQMAGLLGIFGRLGQRRGIQQARRVPDSYLLSLLREPESSIERS"
     gene            complement(2588..3454)
                     /locus_tag="VHS06_08970"
     CDS             complement(2588..3454)
                     /locus_tag="VHS06_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX2988287.1"
                     /translation="MKWDVFISHASEDKDSLVAPLAARLSDLGVKVWYDQFALKIGDS
                     LCRAIDAGLAQSRFGIVIISKSFISKPWPEYELRGLTSREIGHDKVILPIWHGVSRDD
                     VVAFSPSLADKLALDTSTQDQEQIAQGILEVVRPDIFENLHQLAVWKRIRESSERRIT
                     PTASLVPGPIRHDTLPPSLLIRIKNIQHLLAEVDRITLSESIDLFRRDLHPEEEVETW
                     EVIAAAYRDAIADKPYTKQQKSEVFSFFLMASTGTLDADKLNGMVEHLTDDDLSRLVM
                     ILRGVVPEITNQ"
     gene            complement(3550..3792)
                     /locus_tag="VHS06_08975"
     CDS             complement(3550..3792)
                     /locus_tag="VHS06_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX2988288.1"
                     /translation="MKEYRYTIILHPEPEEGGYSVSVPALPGCFAQGDTLEEAVAMAK
                     EAIAVHIEGLLEDGEPIPQEDAPLGMLTISVDAPAA"
     gene            complement(3789..4016)
                     /locus_tag="VHS06_08980"
     CDS             complement(3789..4016)
                     /locus_tag="VHS06_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX2988289.1"
                     /translation="MQKLPRITADDLTRALAKAGWNVVRQKGSHIQLRHPDKKGRVTV
                     ALHAGAIIKPKTLLGTLNQAGMTVEELRRLL"
     gene            complement(4147..4548)
                     /locus_tag="VHS06_08985"
     CDS             complement(4147..4548)
                     /locus_tag="VHS06_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX2988290.1"
                     /translation="MARIRYLSLLDVESLHAFIMERTGDPPSPLRDRGLLEAALMRPQ
                     MAAYYRDADLIEQAAILAVGISQAQSYIQGNKRTAFIVADVFLRLNGGTFHGDPLEMA
                     RRLEDVATWKGNEGEATANLADWLREQVTSG"
     gene            complement(4552..4767)
                     /locus_tag="VHS06_08990"
     CDS             complement(4552..4767)
                     /locus_tag="VHS06_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015214255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX2988291.1"
                     /translation="MVVGKIRKVGNSLVVTVPKEEAEELGLHEGDLVTMDIRKAEVRP
                     VLRPELRKLAEESWARNEAAYRHLREH"
     gene            5147..6184
                     /locus_tag="VHS06_08995"
     CDS             5147..6184
                     /locus_tag="VHS06_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEX2988292.1"
                     /translation="MPLVPMRQILDEAAKGGYGVGAFNVNNMEQIQAIMQAATETQSP
                     VIIQASRGALKYSKLIYLRNLIQAAVEENPNIPVAMHLDHGNSVQTVEQAISLGFTSV
                     MIDGSLGEDGKTPNTYQANVDITKRVVELAHPLGISVEGEIGVLGGIEDEHGAGLAPG
                     STEHLTDPKQAEEFYRLTGVDALAVAVGTSHGAYKSGRKDPVTGQVLPPALAMERIEE
                     IHRLLPHCHMVMHGSSSVPKELVDIINKYGGKMPGTFGIPIEQIQRGIQHGVRKINVD
                     TDIRLAMTGAVRKVLFETPEKFDMRDWMKPARDAAAEVVKARMTQFGAAGHARDYVPI
                     TLEDMKKVYGK"
BASE COUNT         1221 a         1883 c         1925 g         1278 t
ORIGIN      
        1 tagacgtttg gccgccagta ggtcttcggc tccacccact cgatcagtgc ctggttcagc
       61 ttggagagct tcaagaagaa gtgctcggtc gccctgatct caggcgtcgt tccgtcgaac
      121 ttgcagcgag ggttgatcag gtcggtggga tccagcggct tgccgcagtt gtcgcactgg
      181 tccccgcgcg cctccgggta gttgcagaag gggcaggtcc cctccacata ccgatcgggc
      241 aggaagcgtt gctccgaggg gcagaatggc gagaacatct cctgcttgtc gatgtagccg
      301 ttctccagca gccgcaggaa gaggtcctga actacctggt agtggttctc ggtggtcgtt
      361 cgggtgaaga ggtcgtagct gacgcctatc gccttcaggt tctctgagaa ctgctcgtgg
      421 tagaagtcca cgatatcctt gggcgatttc ccctcgcgct cggcgcgcac cgttatgggg
      481 gtgccgtgct cgtcggagcc ggaaaccatc aagacctggt tccccttgac gcgattgaat
      541 ctggcaaaga tgtcaggggg cagcagcgcg ccggccaggt ggcccaggtg cagcggaccg
      601 ttggcgtagg gccaggcaac cccgataaag atgtgctcgc tcatgttctc tccgtcagct
      661 acctacgttc ccgttagtct attaccgttt ccgggttctc tctcggttgc ggcggggtgt
      721 cgtacagccc atttcggtcg ttagaacgca cccgcagcac gtcgcctatc atccgcaggg
      781 tgtcccggac ggggtccacc ctactgctag gtgaataata ccagcttacc ggcacctccg
      841 tgatgccgta gaccagcttc cgtgccaggt agagcacctc cacgtcgaag ccgaagccgt
      901 cgatcacctg tcgcccgaag atatccttcg ccgccttccg agaaaagcac ttgaagccgc
      961 actgggtatc ctggaggtcg ggcatcgtga ggacccggac cagcaggttg aagaccctgc
     1021 ccataaggtg gcggtagcga ggctctccga ttcggcgggc gcccttgacc tccctggagc
     1081 cgatggcgac ctggcagctc tcgcttatgg cggtggggaa gttgggtgtc tcctcgaccg
     1141 gcatggagaa gtcggcgtcg cagaacatga tgtactgccc ccgcgcggcc agcatcccct
     1201 ggcgcacggc gtgacccttg cctctgtggg gctccttcag cagccgcagc tcagggaggc
     1261 tcgggatgga cttttccacg agggcggccg tgccgtcggt gctcccgtcg tcaacgacca
     1321 ggacctcgta ggggaagggc ggccgtttga ggaatgcccg cagcttctcg atggtggcgg
     1381 gcagcctgcc ctcttcgttg taggccggca cgaccagcga caggtaagga cgatccatta
     1441 cgacctctcg atgctggact ctggctcgcg gagcagcgaa agaaggtagc tatccggcac
     1501 tcgccgagcc tgctggatgc cgcgtcgctg gccgagcctg ccgaaaatgc ccagcagacc
     1561 ggccatctgg cctctcagac gagccctggc ggccttctcc cgccaatgac atatagagtt
     1621 taccgcaaaa accgcctggc tcctaacgat tgagggccaa ctgcggcgaa gaatgctcga
     1681 cggcaggttc ttgactgcca cggtgatcaa gtttcggccg cagtagtagc tggccagggg
     1741 ccctcccccc gtcgcactca accgatggta gaccaccgac tgcggaaggt agaggcagcg
     1801 atgccccatc agttgtgccc gcaggccgag gtcgacgtcc tcgcagtaca tgaagagcga
     1861 ctcgtccaga agccccactt tctcgaagag actcttgcgc cagcccgacg cggcaccgca
     1921 cgccagcagg acgaactcct cccggtcgta ctggccggta tccttctgcc acacccctcg
     1981 attgccgggc aatccgtccg tgctgtagaa gtcggccgcc gaatggattg tatccctttg
     2041 gtcgaacagc aggatcttgc aggcgaagaa gtccgactcc gggtgggagc ggcccgcccc
     2101 tgcgagcgtc tccagccagt cctctcgggc ctcggcgtcg ttgttcagca gcgcgacgat
     2161 ctcgcccctg gactcccgga tccccacgtt gacggcgacg gtgaagccct gattgaccgg
     2221 cagggggacg agtcgaaact ccgggtagcg ctcctggacc atccggtcgg agccgtcggt
     2281 ggagccgttg tccacaacca gcacctcgaa gtcgcggaag gactgtgcgc gcagggagtc
     2341 gaggcagccg gccaggtacc gggcaccgtt ccagttgggg atgacgacgg atatgtaagg
     2401 ggctgtctcg gctagctgat cgggcaagat acgggttccc ccgttgcgag gaggtgagcg
     2461 ggcaggccaa gtatatgggt agtgcggaaa ggtggtcaag gaaggggcta ctggagaaga
     2521 tgcacgggtt ccggcatgtt gcatctgtct aacaaatggg agcttgaggt gaggtggatg
     2581 ccgatgttta ctggtttgtg atctctggca ccactcctcg taggatcatc acaagccgag
     2641 aaaggtcgtc atcggtgagg tgctccacca tgccgttcaa cttgtccgca tcaagagtgc
     2701 cagtggacgc cataaggaag aacgagaaga cttctgactt ctgttgtttt gtgtagggct
     2761 tgtctgcgat ggcatcccgg tacgctgccg cgatcacttc ccatgtttca acctcctcct
     2821 caggatgtag atcccgcctg aaaaggtcta tgctctccga tagggtgatg cgatcgactt
     2881 cagcaagaag atgttgaatg ttctttatcc ggatgagtag agatggaggc aaggtatcgt
     2941 gacgtatggg accaggtacg aggctagcag taggcgtgat tctcctctca ctactctcac
     3001 ggatccgctt ccataccgcg agttggtgaa ggttttcgaa aatatcgggc ctgaccactt
     3061 ccaggatgcc ttgggctatt tgctcttgat cttgtgtgga ggtgtctagg gcgagtttgt
     3121 ccgcgagcga aggactgaag gcgacgacgt catctctgga gactccgtgc catatgggca
     3181 ggatgacctt atcgtgtcca atctctcggc ttgtcagccc acgcagttcg tactcaggcc
     3241 atggcttgct gatgaatgac ttgctgatga tgacgatgcc gaacctggat tgagccagcc
     3301 cggcatcgat ggccctgcac aggctgtcgc cgattttgag agcaaactgg tcgtaccaaa
     3361 ccttgacccc gagatcggat agcctggctg caagtggagc taccagagag tctttgtctt
     3421 cggaggcatg cgagataaag acgtcccact tcatctatcg aatctcagta atgtccgagt
     3481 tgcccgatat aaacgagtga gggagacact atactgggaa gtcgaattct cacccatcca
     3541 ctccatgtcc tatgctgccg gcgcatccac gctgatggtc agcatcccta gcggagcatc
     3601 ctcctggggg atcggctcgc cgtcttcgag aagaccctct atgtggacgg caatggcttc
     3661 cttcgccata gcgacggcct cttccaaggt gtcgccctgt gcaaagcaac ccgggagggc
     3721 cggaacgctc acggagtagc caccctcttc gggctcgggg tgcaggatga tggtgtatcg
     3781 gtattctttc acagaagtct cctcaactct tccacggtca tgcctgcttg gttgagcgta
     3841 cccaaaaggg tctttggctt gattatagcc ccagcgtgaa gggcgacggt aacacgtccc
     3901 ttcttgtccg gatgtctcag ttggatgtgg ctacccttct gcctaaccac attccatccg
     3961 gccttggcta gggctcgggt caaatcgtca gccgttatgc gcggtaattt ctgcactctc
     4021 gctcaacctc cccttactgg aagagcggaa acctggtata tgttcaacaa agatcaaagt
     4081 ggcagaattg tagcacagca ggagtagcgc aggcgggatg tgggcttggc ttggcgaacg
     4141 ttcttactac ccggaggtca cctgctcccg cagccaatcg gcgaggttgg ctgtagcttc
     4201 accctcgttc cctttccagg ttgcgacgtc ctccagccgg cgagccatct ccagcggatc
     4261 cccatggaag gtgccgccgt tgagccggag gaagacatcg gcgacgataa acgccgttcg
     4321 cttgttgccc tggatgtatg actgcgcctg ggagatgccg acggcaagaa tggcggcctg
     4381 ctcgatcagg tccgcatccc tgtagtaggc ggccatttgc ggacgcatga gcgccgcctc
     4441 caggagccct cggtcgcgga gtggggacgg tggatccccc gtcctctcca tgatgaacgc
     4501 gtgcagagac tccacgtcga gaaggctcag gtagcgaatg cgggccatgg gttagtgttc
     4561 tcgaagatgg cgataggcag cctcgttgcg cgcccagctc tcctctgcca gctttcggag
     4621 ttctgggcgc aggacgggcc gaacctcggc cttccggatg tccatggtca ccagatcgcc
     4681 ctcatgcagt cccagttcct ccgcctcttc tttggggacg gtgactacca ggctgtttcc
     4741 gaccttgcgg atctttccaa cgaccatctc gtgcctccgc atggaagtcg aatcgtatgc
     4801 accacattac caagtatata ctgtgttacc tgaaaccgca caacagatgc cgagcatggc
     4861 ctcatcgttc atgtgtgtgg ggctgctcgg tgacgacgcg atcgggaacg gccgtctggc
     4921 gtcaccgtcg acagatccgg ctaaagcctc gactccagtt gttggctgtg accagcaccc
     4981 agcactcagc actcagcgcc caccgccgtg gccccaagcc tgctgtaacg atgtctgcaa
     5041 aactctccag atcgcccacc aggccgagaa ctggccggtc agaggttcaa tttggtagga
     5101 taggacagcc ccctttgaga ggttttagcg gaaaggagtc gtcacaatgc ctctagtacc
     5161 gatgcgacag atcctcgacg aggctgccaa gggtggctac ggtgttggcg cgttcaacgt
     5221 caacaacatg gagcagattc aggccattat gcaggccgcg accgagaccc agagcccggt
     5281 gatcatccag gccagccgcg gcgcgctgaa gtacagtaag ttgatctatc tgcgcaatct
     5341 gatccaggcc gccgtcgagg aaaaccccaa cattccagtt gccatgcacc tggaccacgg
     5401 caacagcgtg cagaccgtcg aacaggccat ctccctcggc ttcacctccg tgatgatcga
     5461 tggctcactg ggcgaggacg gaaagacccc caatacctat caggccaacg tggatatcac
     5521 caagagggtc gtcgagctgg cccacccgct ggggatcagc gtggagggcg agatcggcgt
     5581 gctgggcggt atcgaggacg agcatggtgc ggggttggcc ccgggttcca ccgagcacct
     5641 gaccgatccc aagcaggccg aggagttcta ccggctgacc ggtgtggatg cgctggccgt
     5701 tgcggtaggc accagccacg gtgcctacaa gagcgggcgc aaggatccgg tgaccggtca
     5761 ggtgttgccg cctgcgctgg ccatggagcg gatcgaggag atccatcgcc tcctgcctca
     5821 ctgccacatg gtgatgcacg gctctagctc agtcccgaag gagcttgtgg acatcatcaa
     5881 caagtatggt ggcaagatgc ccggcacctt cggcattccg atcgagcaga tccaacgcgg
     5941 aatccagcac ggcgtgcgca agatcaacgt ggataccgat atccgtctgg ccatgaccgg
     6001 cgccgtccgc aaggtgctct tcgagacgcc agagaagttc gacatgcgcg actggatgaa
     6061 gccggcgcgc gacgctgcgg cggaggtggt caaggcgcgc atgacccagt tcggcgcggc
     6121 cggccacgcc cgagactacg tccccataac gctggaggac atgaagaagg tctacggcaa
     6181 gtagtaatac ctgctcgcat cccttcatac tttggagtcg agatgccggg gtcgttcccg
     6241 accccggcgt tccgcgctag gcggccttgg tctcaccccc ggcctccgcc acgggcaggc
     6301 tgaagct
//
LOCUS       DATALS010000402         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_426553, whole genome shotgun sequence.
ACCESSION   DATALS010000402 DATALS010000000
VERSION     DATALS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426553"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..1417)
                     /gene="ltaE"
                     /locus_tag="VHS06_09000"
     CDS             complement(377..1417)
                     /gene="ltaE"
                     /locus_tag="VHS06_09000"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEX2988293.1"
                     /translation="MVAVIDLRSDTVTHPTPEMREAMYRAEVGDDVYGEDPTVERLQK
                     MAAERLGKEAALFTTSGTQSNLVGVLTHCGRGDEVIVGDQAHIFHYEVGGVSALGGIM
                     VRTVANRSGAPDPADVLAALRDPANVHHTISRLVCLENTHNRCGGVALTREETVAVAD
                     VAHEHGLKVHLDGARLFNAAIALGVDARTLAEPCDSVNICLSKGLCAPVGSLLVGSAE
                     FIARARRNRKMVGGGMRQVGVLAAAGIVALEKMVDRLAEDHANARVLAETLAGLPGMK
                     VNLKAVQTNIVVAELDRQDMNVAQFLARAKEQGVKISPFGGNKVRFVTHHGITQQDVE
                     TACRVVKEILAA"
     gene            1610..1864
                     /locus_tag="VHS06_09005"
     CDS             1610..1864
                     /locus_tag="VHS06_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988294.1"
                     /translation="MTLLSWIAIGLVVGTGWRLVARRYGLLEDAIVGVVGAVVFGWLF
                     VLFTGASVVAVDPEGLLFSLLGSTLSVALARGISRGRATI"
     gene            1962..>3156
                     /locus_tag="VHS06_09010"
     CDS             1962..>3156
                     /locus_tag="VHS06_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2988295.1"
                     /translation="MPKCVLMLRTSLLLAALAIATLPVTACNLKLPPAENSKPEARVL
                     QPEPTREAAAAPTALPTRPAFADPATQAAPKEPTQTPAAVQVASSIAPQPGAGLSVVQ
                     VSARVRPAVAQIMVKGTTLSLFDPVPTSGVGSGAVIDKKGYIITNNHVVEDATDIVVS
                     LPEREKTYTGHLVGRDPSTDLAVIQIEADNLPTVGLGDSDSLQIGEPVVAIGNALGLE
                     GGPTVTSGVVSALNRSIDEPSGATLNQLIQTDAAINPGNSGGPLVNLSGQVIGVNTAV
                     ATGSSDAPAQGIGFAISINSAKPIIDDLIAYGKVRRPYLGVELATVTPSLARQYRLAV
                     DRGVYIVRTVAGSPAAKAGITAGDIVTKIDSDDIRTVADLRKSIAKAKIGQKIKIQVA
                     RGSSQR"
BASE COUNT          602 a         1077 c          908 g          569 t
ORIGIN      
        1 gtcgcgcttg tgccccgcct cccacggtgc ggccacgacc agcacggtga ttacgacctc
       61 gcaaatggcg gggcacaaga ccccgcccta cgagacggcg atggaggcgg cttgcccgca
      121 tcagcgtgat atggccacgc gtggggggga cggattcaaa caggatagac aggatgtaca
      181 ggatgagaat cgaaatcgtg aatccccatc ctgtctatcc gtctcatcct gtatatcctg
      241 ttcgtttcct ctgtgcgagc cttcggcccg cctacgggcg agacggccac agcctcagga
      301 ttgaccatgc catcatgctc cggagcggta tctcccccgt ccccagcacc cagcactcag
      361 cacccagcac ttcgcgttag gctgccagga tctccttcac cacgcggcag gcagtctcca
      421 cgtcctgctg agtgatcccg tggtgggtga cgaaacgaac cttgttcccg ccgaaggggg
      481 atatcttcac tccttgctct ttcgctcgag caaggaactg ggccacgttc atatcctggc
      541 gatccagctc tgccacaact atgttggtct gaacggcctt caggttcacc ttcatgccgg
      601 gcaaaccggc caacgtctcg gccagcacgc gagcgttggc gtggtcctcg gccaacctat
      661 ccaccatctt ctccagcgcc acgatcccgg cggctgccag cacacccacc tggcgcatcc
      721 cgccgccgac catcttacgg tttcggcgag cacgagcgat gaactcggcg gaacccacaa
      781 gcagcgaacc aaccggggca cacagcccct tgctcaggca gatgttcacc gagtcgcacg
      841 gctcggccag cgttcgtgca tccaccccca gcgcgatggc agcgttgaac aggcgagcgc
      901 cgtccaggtg aaccttcagc ccgtgctcgt gagctacatc ggcaacggcc acggtctcct
      961 cacgcgtgag cgcgacacct ccgcagcgat tgtgggtgtt ctccaggcat accagccgtg
     1021 atatagtatg atggacgttg gcaggatccc tcagtgccgc cagcacgtcg gcgggatcgg
     1081 gcgcacccga ccggttcgcg accgttcgga ccatgatgcc acccagggcc gagacaccac
     1141 cgacctcgta gtggaagatg tgggcctgat ctccaactat cacctcgtcg ccccggccac
     1201 agtgggtaag gactcccacc aggttgctct gtgtgccgct cgtggtgaag agtgcagcct
     1261 ccttgcccaa tctctccgcg gccattttct gcagccgctc cacagtgggg tcctctccgt
     1321 agacatcgtc ccccacttct gcccgataca tcgcctctcg catttccggc gtcgggtggg
     1381 tcaccgtgtc gctgcgaagg tcgattactg caaccatact gcgctgctcc tctagggatg
     1441 gattccgaag attatagcag gcacgctctc tgctttctct tctaccttcg cctcgcgagt
     1501 gacccggccg gcaacccggt ccttcaatag ggtggtcttc gcatatggga cacgacccat
     1561 cgggcacggg ccaaacgtct cgcccgtatg aaagcagaag gcagacagaa tgacgctgct
     1621 atcctggatc gcgatagggc tggtggtggg taccggatgg cggctcgtag ccaggcgcta
     1681 cgggctgctg gaagacgcaa tcgttggagt tgtgggggca gtggtcttcg gctggctgtt
     1741 cgttctcttc accggtgcct cggtcgtagc agtcgaccct gaagggctcc tcttctcgct
     1801 gctgggctcc accctctcgg ttgccctggc ccgaggcatc agccggggac gcgcaaccat
     1861 ttaggtccca attcgagaca cctcgaagct gatatactag ccgtaccttc gccgcccggc
     1921 gcggcaccta ccctgttgac taccgatggg agtccttacg tatgcccaaa tgcgtcttga
     1981 tgctgcgcac ctcactgctc ctagccgccc tcgccatcgc cacccttcca gtaaccgcct
     2041 gcaacctgaa gctaccgccg gcagagaaca gcaaacctga agcccgtgtg ctgcaaccgg
     2101 aaccaacccg cgaagcggcc gccgcaccca cggcgcttcc tacacgaccg gcctttgcgg
     2161 atcctgccac gcaggccgca cccaaagagc ccactcagac gcccgctgcc gtccaagtcg
     2221 cttcgtccat tgcgccccaa ccgggagcgg gattgagcgt cgtgcaggtg tccgccaggg
     2281 tgaggcccgc agtcgcccag atcatggtca agggcaccac cctcagcctc ttcgatccgg
     2341 tccccactag cggcgtgggc tcgggtgcgg tgatagataa gaaaggctac atcattacca
     2401 acaaccacgt cgtcgaggat gccaccgata tagtggtttc cctcccagaa agggagaaga
     2461 cctacacggg ccatctcgtc ggccgcgacc catcaacgga cctggcggtg atccagatcg
     2521 aggcagacaa cctgcccacg gtcggcctgg gcgactcaga ttcgctccag atcggtgaac
     2581 ctgtagtggc gatcggaaac gcgctggggc tggagggtgg ccccacagtg acgtcgggcg
     2641 tggtcagcgc actgaaccgc agcatcgacg agccctccgg cgccacgctg aaccagttga
     2701 tccagaccga tgcagccatc aatccgggca atagtggtgg ccccctcgtc aacttgagcg
     2761 ggcaggtgat cggggtgaac accgccgtgg ccaccggctc cagcgacgcg ccggcacagg
     2821 ggatcgggtt cgcaatttcc atcaacagcg ccaagcccat catcgacgac ctgatcgcct
     2881 acggcaaggt ccgccggcca taccttgggg tagaactggc cacggtaacc ccctcgctgg
     2941 cgcgccagta caggttggcg gtggatcgtg gagtctacat cgtcaggacg gttgccggaa
     3001 gccccgctgc caaggccggc atcacggcag gagacatagt caccaagatc gactccgacg
     3061 acatccgaac cgtcgcggac ctgcgcaagt ccatcgccaa ggccaagatc ggccagaaga
     3121 tcaagatcca ggtcgcccgc ggctccagtc agcgat
//
LOCUS       DATALS010000403         6057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_65481,
            whole genome shotgun sequence.
ACCESSION   DATALS010000403 DATALS010000000
VERSION     DATALS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65481"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1444
                     /locus_tag="VHS06_09015"
     CDS             257..1444
                     /locus_tag="VHS06_09015"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEX2988296.1"
                     /translation="MRKIVSRLLLMPLAALVAMAVGAAANPPAVAQAQTQGVYLSTAV
                     PGVVVDKGKQVTFSIDVNNKGASGQTVDLALVKAPDGWNPILKDRGFVVRSVWVDAGK
                     SEAVDLQIKAPSEAKAQDYEFQLRASVGGVEISTLRLLVGIKDQPTQGTSFVVQYPTL
                     KGKSGASFGFKAELHNDSEEDRTYGLSFKAPDGWDVSFKPSYEDKQISTVQVKTGASQ
                     NLDVSVTPPARVEAGDYPITLTASSQADKANTDLKVTITGSYQMDVSTRTGLFNTSAT
                     AGQDSPFYVTVTNTGSAPLQGVSLSSSKPDGWTVTFSPDKIDQLDPGAQREVTMTIKP
                     SAKAIAGDYVLNLTTTHPQVSVNKDIRVTVETPTVWGWVGVIILVAVIGGLMVIYMKL
                     GRR"
     gene            1449..2393
                     /locus_tag="VHS06_09020"
     CDS             1449..2393
                     /locus_tag="VHS06_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988297.1"
                     /translation="MDERSQIAIETVDLTKKYGELTAVNKLNLSISEGEIFGLLGPNG
                     AGKTTTILMLLGLTEPTSGKARVCGHDSTREPLAVKRMVGYLPDNVGFYEDLTAVENM
                     LYTADLNQIPRKEALERIYNLLGRVGLADVADKEVGKFSRGMRQRLGIADVLVKKPKI
                     VILDEPTLGIDPDGVKQMLDIIVQMSKEDKITVLLSSHLLHQVQQICSKVGIFVKGRM
                     VAEGSIEMLGLQILGGDPIVVEAEVAGVDDALIGALKKIPGVSEVEQSAGNLILHCFK
                     DVRGEIAKCIVQAGKSMSTLRLRGHVLEEIYLRYFQEA"
     gene            2397..3344
                     /locus_tag="VHS06_09025"
     CDS             2397..3344
                     /locus_tag="VHS06_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2988298.1"
                     /translation="MPGLIAVFRKEMADNFASKRFLIMFALILLAGMSATYTAAQAIR
                     TAVSGSSSGSVSFVFLLLFTVSDGMLPSFISFISFLGPLVGLSMGFDAINGEYARGTL
                     SRVLSHPIFRDAVINGKFLARLATIAVMLVCISMVLAGLGLRLIGVPPTADEIFRLLL
                     YMGISIVYVAFWMSLAILFSILFRQATTSALAGIAVWIFFTFFMGMIAGVAAQAIAPV
                     NQQDPQTVIDQVHASQAIARLSPGTLYQEITSIILDPSSRTLSPVVMASEQQGMVAGI
                     LPLGQSLLLVWPHIVALIAETVICFAISYVKFMRQEIRA"
     gene            complement(3434..3775)
                     /locus_tag="VHS06_09030"
     CDS             complement(3434..3775)
                     /locus_tag="VHS06_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012959405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX2988299.1"
                     /translation="MSNRDGGREEGEKPTDPGRGNSLPLARPQAVALSYDPARARAPK
                     ITAQGSGTVAERIIQLARQHEVPIKEDPLLVQALSKLEVGQTIPPQLYQVVAEVFAWL
                     YRLDQEQAAEG"
     gene            complement(3840..5627)
                     /locus_tag="VHS06_09035"
     CDS             complement(3840..5627)
                     /locus_tag="VHS06_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX2988300.1"
                     /translation="MDLGPMSIPPLLPGPIRGESDPTGQGLAPRNSTADSSAGMQRQG
                     LPVDIAGRFAQMQAEMAMEAGVPPRAAPQAGSSQLSSAEGVAMPAGSTGAQGATPSAG
                     LAKTEGTTVSAGATTLEIEAEPQLISALRRLGLGVDRASLNAVRLLMSAGRPVTREDV
                     GCLIRWQVTSPYVGRDQPSADGILAEILRTWGVPADSAHLEAVRQMLRRGLSKESLQR
                     LAREQVPSGTEQEQQTSDSILGQILHAYDLPDDPPHISAAREFLARSLPLSREAIDRL
                     ARTLSRIGATEEEDFRAAVFLQANDLPVTESTIHLARSFLERPLQLAQQLRQLQMAVT
                     EAAVALLATPQEDDLGPLLDRAAGQLSQRLLSAQAGDRQAIVDTVRRLFADQGISLEN
                     RLARVLAGGDPSQLEGDLRTLLGRLADMAMKAGAAASQHPDLQSALAQLSQSAPELAD
                     ALQTQQLANAATPASQLDQWIVFQLPVSVGDRDDPRTAELRISKRPGRKIDPDHVRLL
                     LRLDLPRLQMVEIGLQIVGPRVGCSLSSRNEETLPLLREQFGALRQGLEQLGYSVARP
                     SFSLLSKEPPATEPAPVVPARLGRLDLRA"
BASE COUNT         1120 a         1955 c         1855 g         1127 t
ORIGIN      
        1 atatactatt ccggtacaag atgttgttag ttggggcagc cctaagggct gcccttggcg
       61 ggcacgacgg acgcccaggt gagcgtccat cgtgcggcgg ggcggcattt actgttcctc
      121 ctgacgggcc gctcgccgac actctctctg cgtaaagtgt tgctacacct gtgttctttg
      181 acgtccgcca aacggcggcg tccggcaata atggctttcc gattctctct agagacaagt
      241 tggaggtgat cgtacagtga ggaagatcgt gtccagattg ctgctcatgc cgctagcggc
      301 actggtggcc atggccgttg gcgccgccgc caacccaccc gccgtggcgc aggcgcagac
      361 tcagggggtt tatctctcca ccgcagtccc cggcgtggtc gtggacaagg gaaagcaggt
      421 gaccttcagc atcgacgtca acaacaaggg agcgtcgggc cagaccgtcg acctggctct
      481 tgtcaaggct ccggacggat ggaatccgat attgaaggac cgaggcttcg tggttcgctc
      541 cgtctgggtt gatgcgggca agtccgaggc cgttgatctg cagatcaagg ccccgtccga
      601 ggcaaaggcc caggactacg agttccagtt gagggcttct gtgggcggcg tcgagataag
      661 caccctgcga ctgctggttg ggatcaagga tcagcccacc cagggcacct ctttcgtcgt
      721 gcagtacccc acactgaagg gcaagtccgg tgcatccttc ggcttcaagg ccgagcttca
      781 caacgactcc gaggaagacc gcacatacgg gctcagcttc aaggcccccg atggatggga
      841 cgtgagcttc aagccctcct atgaggacaa gcagatctcg accgtgcagg tgaagacggg
      901 cgcatctcag aacctggacg tcagtgttac accgcccgcc agagtagagg ccggcgacta
      961 cccgattacc ctgacggcat cgtcccaggc cgacaaggcg aacactgatc tgaaggtcac
     1021 catcaccggc tcctaccaga tggacgtgag cacgcggacc ggcctgttca acaccagcgc
     1081 caccgccggc caggatagtc ccttctacgt gaccgtcacc aacaccggct cggcgccgct
     1141 gcagggggtc agcctctcct ccagcaagcc agatgggtgg actgtgacct tcagcccgga
     1201 caagatcgac cagctagacc cgggtgcgca gcgtgaagtc accatgacca tcaagcccag
     1261 cgcaaaggcg atcgccggag actacgtgct gaacctgacc accacccacc cgcaggtgtc
     1321 cgtcaacaag gacatccggg tgaccgtcga aaccccgacc gtctggggct gggtaggtgt
     1381 gatcatcctg gttgcggtaa tcggtggtct gatggtcatc tacatgaagc taggccggag
     1441 atagcataat ggacgagcgg tcgcagattg ctatagagac cgtcgacctg accaagaagt
     1501 atggcgagtt gacggcagtc aacaagctga acctctccat atcggaaggg gagatcttcg
     1561 gcctgctcgg ccccaacggc gctgggaaga cgaccaccat cctgatgttg ctggggctga
     1621 cggagcccac ctcggggaag gccagggtct gcgggcacga ctccacgcgc gagccgctgg
     1681 cggtcaagcg catggtcggc tacctgcccg acaacgtggg gttctacgag gacctgacgg
     1741 cagtcgagaa catgctctac accgccgatc tgaaccagat acctcggaag gaggcgctgg
     1801 agcggatcta caacctgttg ggcagggtcg gcctggccga cgttgccgac aaggaggtgg
     1861 gcaagttctc ccgtggaatg cgccagaggc tcggcatcgc cgacgtgctg gtcaagaagc
     1921 ccaagatagt gatcctcgac gagcccacgc tgggcatcga cccggatggc gtgaagcaga
     1981 tgctggacat catcgtccag atgagcaagg aggacaagat caccgtcctg ctctcctccc
     2041 acctgctgca tcaggtgcag cagatctgct ccaaggtcgg gatcttcgtc aagggccgca
     2101 tggttgcaga gggctccatc gagatgctgg gcctccagat acttgggggc gacccgatcg
     2161 tggtggaagc cgaggtcgcg ggcgtcgacg acgccctgat cggggctctc aagaagatac
     2221 cgggggtttc cgaggtagag caaagcgcgg gcaacctcat cctgcactgc ttcaaggacg
     2281 tgcgtggcga aattgccaag tgcatcgtcc aagccggcaa gtcgatgtct actctacgac
     2341 ttcgcggcca cgtgctggag gagatctacc tcaggtactt ccaggaggct taagatatgc
     2401 caggattgat agcggtcttc cgcaaggaaa tggccgacaa cttcgccagc aagcgatttc
     2461 tgatcatgtt cgcgctgatc ctgctggcag gcatgtctgc cacctatacc gcagcgcagg
     2521 ccatccgcac cgccgtatcc ggcagcagca gcggctctgt ttccttcgtc ttcctgctgc
     2581 tcttcacggt gtccgatggt atgctcccgt ccttcatctc cttcatcagc ttcctcggtc
     2641 cgctggttgg gctgtcgatg gggttcgatg cgatcaacgg ggaatacgct cgcggtaccc
     2701 tgagcagggt gctttcgcac ccgatcttcc gcgacgcggt catcaacggc aagtttctcg
     2761 cgcggctggc caccatcgcc gtcatgctgg tgtgtatctc catggtgctg gcgggcctgg
     2821 ggctgcggtt gatcggcgtt ccgcccacgg cggatgagat cttcaggctg ctgctgtaca
     2881 tggggatctc catcgtgtac gtggcgttct ggatgagtct tgcgatcctc ttctcgatcc
     2941 tgttccgcca agcgaccacc tcggcgctgg cagggatcgc ggtgtggatc ttcttcacat
     3001 tcttcatggg catgatcgcc ggtgtcgcgg ctcaggctat cgcgccggtg aaccagcagg
     3061 atccgcagac ggtcatcgac caggtgcacg catcccaggc catagcgagg ctctcgccgg
     3121 gaacgctgta tcaggagatc acgtcgatca tcctggatcc tagctctcgg acgctgtcac
     3181 ccgtggtgat ggcgagcgag cagcaaggca tggtggcggg catactgccg ctgggccaga
     3241 gcctgctgct ggtgtggccg cacatcgtgg cacttatcgc ggagacggtg atctgcttcg
     3301 ccatctccta cgtgaagttc atgcgccagg agatcagggc gtagcgctct gacacggtgg
     3361 cgttgccctt taggagcccg ggtcgtccct gacccgggct ctctttcgtt gtgacacgct
     3421 aagggcggca gccctacccc tccgccgcct gctcctggtc cagccggtag agccaggcaa
     3481 acacctccgc caccacctgg tacagttggg gagggatggt ctggcccacc tccagcttgg
     3541 agagcgcctg caccagcagc gggtcctcct tgatcggcac ctcgtgctgc ctggccaact
     3601 ggatgatccg ctcggccacg gtcccgctgc cctgggcggt tatcttaggc gcccgcgccc
     3661 tggcaggatc gtagctgagg gctaccgcct gagggcgggc gagcggaagg gaattccccc
     3721 tacccggatc ggttggtttc tccccctctt ctctgccgcc gtccctgttg gacaccgtta
     3781 catcctccca tctcgaaacc cgtcgagaca gatggcctca tctcagaggc attcctcggc
     3841 tacgccctca ggtccaaccg ccccagccga gcgggcacca ccggcgctgg ctcggttgcc
     3901 gggggctcct tcgaaagaag ggagaaggag gggcgcgcga ccgagtagcc caactgctcc
     3961 agcccttgtc tcagggcacc aaactgctcc cgcagtagcg gcagcgtctc ctcattcctg
     4021 ctggagagac tgcagccgac ccgcggtccc acgatctgca gcccaatctc caccatctgc
     4081 agccgaggca ggtccagcct gagcagcagc cggacgtggt ccgggtcgat cttgcgcccc
     4141 ggccgcttgc tgattcgcag ctccgccgtc cgagggtcgt cccggtctcc cacggacacc
     4201 ggcagctgga agactatcca ctggtccagt tgcgatgccg gcgtggcggc gttggcaagc
     4261 tgttgtgtct gcaaagcgtc ggccagttcc ggagcgctct ggctcaactg tgccaacgcg
     4321 ctctgaagat ctgggtgctg cgatgccgcc gcccccgcct tcatcgccat gtcggcgagc
     4381 cgccccagta gagtgcgcag gtccccctcc aactgggacg gatcacctcc ggccaggaca
     4441 cgagccagcc ggttctccag gctgattccc tgatccgcaa agagacggcg caccgtatcg
     4501 acgatcgcct gtcggtcccc agcctgggcc gagagaagcc gctgagacag ctgtccggcc
     4561 gctcgatcca gcaatggtcc cagatcgtcc tcctgagggg tcgccagcag cgctaccgcg
     4621 gcctccgtga cggccatctg aagctgccgc agttgctgcg ccaactggag aggtcgctcc
     4681 agaaagctcc tcgccaggtg gatggtggat tcagtgacgg gcaagtcgtt cgcctggagg
     4741 aacacggccg ctcggaaatc ctcttcctcg gtagccccga tcctggagag ggtgcgtgcc
     4801 agccgatcta tcgcctcgcg ggacaacggc aggctccgcg ccaggaactc tcgagcagcg
     4861 gatatgtgcg gcggatcatc cggaagatcg tacgcatgga ggatctgtcc caggatcgag
     4921 tccgaggttt gctgctcctg ctctgtcccc gaaggcacct gctcgcgagc caacctctgc
     4981 aggctctcct tcgaaagccc ccgtctcaac atctgtctga cagcctcgag atgggcagaa
     5041 tcggccggta ctccccatgt acgaaggatc tcggcaagga tcccatctgc cgacggctga
     5101 tcgcgtccaa cgtacgggct agtgacttgc cagcgaatca ggcagccgac atcctcccgg
     5161 gtgacaggcc gtccggctga catcaacagc cgcacggcgt tcagagaggc acggtccacc
     5221 cccagaccaa gccggcggag tgcacttatc agttgaggct cggcttcaat ctccagtgtg
     5281 gtcgcgcctg ccgacactgt cgtgccctcc gtcttggcca acccggcgga cggggtcgca
     5341 ccctgcgccc cggtcgaccc ggcgggcata gccacaccct ccgcgctgga cagttgggaa
     5401 ctgccggctt gcggtgccgc cctaggcggg acgcctgcct ccatcgccat ctcggcctgc
     5461 atctgcgcga accgccctgc aatatccacc ggcaggccct gtcgctgcat ccctgcagac
     5521 gagtcggccg tcgagttccg cggcgccaga ccctgtccgg tcgggtccga ttcgccacgg
     5581 atgggaccgg gcaggagggg gggaatgctc atcgggccaa gatccacaca gcacctccaa
     5641 aggtccctca aacgtcccga acgaagacaa ttcgcaagaa gagagaatcc gctttgtcat
     5701 atagtatcgg ccgttttctt cacatcttga gtggcggtga catccactcg gcaaatctcg
     5761 ccatgcttct cagatcggac tccgggctcg acatccaact gctactggtc cccctttgcc
     5821 ccggcgcgaa caatctccgg cggtggcgct ataattgatg ggcgcggagc gcaactctcc
     5881 ggcaagactt cgtatgtaag gggggacgat gaaggcaacc agagcaatgg agatcctgga
     5941 gaaggcccgc gtcccgtacg aggtccgatc cttcgagcac gacggcttca cccccgccaa
     6001 ggaggcggcc gagcggctca ccatgtcccc cgacaccttg ttcaagacac tgatcgt
//
LOCUS       DATALS010000404         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_270223, whole genome shotgun sequence.
ACCESSION   DATALS010000404 DATALS010000000
VERSION     DATALS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270223"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VHS06_09040"
     CDS             <1..595
                     /locus_tag="VHS06_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2988301.1"
                     /translation="VAVGADPSGEMIRLAEERAAGRSGVRFVQAGFGELADRVGSDFD
                     VLTCLGNTLPHTLTRHDLDNALADMAAVLRPGGMLVIQMLNYDLILAEQRRFLGVSSG
                     VRGGVEYLFFRFYDFDGLRLTFNVATFKREGEGWSFNVDSTQLRAITQEELADALTQA
                     GYQEPRWYGGLDRSEFDPASSGDLVAVAYKPERHPQP"
     gene            complement(656..1468)
                     /locus_tag="VHS06_09045"
     CDS             complement(656..1468)
                     /locus_tag="VHS06_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2988302.1"
                     /translation="MKIGDFSKLTQLSVKALRYYDEIGLLKPTEVDRFTGYRYYSVQQ
                     LPRLNRLLALKDLGLSLDQIANMLDGDLPAAQIRGMLRLKQAEIQQRVDEERGRLARV
                     EARLREIEREGTMSNYDVVTKKIEPQLVASLRRVVPSYGQVGPLFGELYATAGRRGLK
                     PTDAPIAVYYDPEYREKDVDVEACAPIDPSSAQQAPDIVRELPGIDTAACILHQGSYE
                     TLVNSYTALHQWLEANGYQICGPNREIYLHTPEMEGGNSAMMTEIQFPIEKR"
BASE COUNT          292 a          512 c          537 g          359 t
ORIGIN      
        1 ggttgcggtg ggagccgacc cttccggtga gatgatccgc ctggcggagg agcgggcggc
       61 aggcaggtcg ggagtgcgct tcgtccaggc gggctttggt gagctggccg accgggtggg
      121 aagcgatttc gacgtcctga cctgtctggg gaatacgctc ccccataccc tgaccaggca
      181 cgatctggac aacgccctgg cagacatggc tgccgtgctg cgtccgggtg ggatgctggt
      241 gatccagatg ttgaactatg acctgatcct tgcggagcag cgacgattcc tcggcgtctc
      301 ctcgggcgtg cggggcgggg tagagtacct gttctttcgc ttctacgact tcgacggtct
      361 caggctgacc ttcaacgtcg ccaccttcaa gcgggaaggg gagggatgga gtttcaacgt
      421 ggactccacg cagcttcgag cgatcacgca ggaggagttg gccgacgcgt tgacacaggc
      481 gggctaccag gagccgcggt ggtacggtgg cctggacagg tcggagttcg atcccgcctc
      541 ctccggcgac ctggtggcag tggcctacaa gccggagcgg cacccacagc cgtagtatcg
      601 gccgtttcct gcaggggagg tcgaaaacga cccgccctgc aggaagcatc ttctcctacc
      661 gcttctcgat ggggaattgg atctccgtca tcatcgcgga gttgccgcct tccatttccg
      721 gtgtgtgcag gtatatctca cggttcgggc cgcagatctg gtacccgtta gcctccagcc
      781 actggtggag cgcggtgtag ctgttcacca gtgtctcgta acttccttga tggagtatgc
      841 aggctgccgt atctatgccc ggcagctcgc ggactatgtc cggggcctgc tgggcggaag
      901 atggatcgat cggggcgcag gcctccacgt ccacgtcctt ctcacggtac tccgggtcgt
      961 agtagacggc gatcggggca tcggtcggct tcaatcctct ccgccctgcc gttgcataca
     1021 gttcgccgaa cagcgggccc acctgaccgt agcttgggac cacccggcgc aatgaggcca
     1081 ccagttgcgg ttcgatcttc ttggtcacca cgtcgtagtt ggacatagta ccctcccttt
     1141 cgatctctct cagccgtgcc tccaccctgg caagccttcc ccgctcctca tccacacgct
     1201 gctggatctc cgcctgcttt agtcgtagca tcccgcggat ttgggccgcg ggtagatcac
     1261 cgtccagcat gttggcaatc tgatccagcg agagccccag atccttgagc gccagcagcc
     1321 tgttcagccg aggcagttgc tggaccgagt agtagcggta ccccgtgaag cggtccacct
     1381 cagtcggttt cagcagcccg atctcgtcgt agtagcggag cgccttgact gagagttgag
     1441 tgagcttcga gaaatcaccg atcttcaaag tgctacctcc gcgcgcggta ggtcattgga
     1501 agcttcgacg ggacaataca ccctcccgca aggggagagt caagagatgg gttttttgcg
     1561 acgctcgcga catcccgcga accctgtgat caacttacct gcattagtgg gcgtacacgc
     1621 cgcttgcctc ttctgctcgc gagtcggtat attgggagtt ccaccgcgcg gacaagttgg
     1681 agaagtgggc gagggataga
//
LOCUS       DATALS010000405         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_336820, whole genome shotgun sequence.
ACCESSION   DATALS010000405 DATALS010000000
VERSION     DATALS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336820"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VHS06_09050"
     CDS             <1..189
                     /locus_tag="VHS06_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012952435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988303.1"
                     /translation="VVRHLGKALQPREVRFARELPKTRNGKIMRRVIRAAYLGKEEMG
                     DLSSLENPAAVEEIARAA"
     gene            409..1134
                     /locus_tag="VHS06_09055"
     CDS             409..1134
                     /locus_tag="VHS06_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007979458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2988304.1"
                     /translation="MMTLLDRERGRWRESSREGEMGGFSRGWVFGAVTGIAAVSAYRM
                     AIRPWHLRWGATETEASWQLPGDELVPEPKINATHAVTIDAPPEAVWPWIAQLGQGRG
                     GFYSYDWLENLMGLDIHSADSVIPEHQQIKEGDGIPLAPGGFDFPVVKVEPQRMLLLH
                     GDTRDGSSPVPVKPGEYLNASWLFLLDAAGRGGTRLVERFRADYDPGLVKGLVYGVFV
                     EPGAFLMERKMLLGIKERAEQLA"
     gene            complement(1299..>1850)
                     /locus_tag="VHS06_09060"
     CDS             complement(1299..>1850)
                     /locus_tag="VHS06_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEX2988305.1"
                     /translation="PVSGIQVLSPMYRGKVGVDFLNEELQKKLNPPHQDKPEKRFGGK
                     LFRLGDKVMQIRNNYDKQVYNGDAGRITAIDLEEQLVLVAFDDDRLVEYEFSELDELV
                     HAFAISVHKAQGAEYPAVVIPVLTQHYLLLQRNLIYTAITRAKELVVLVGTKRALAMA
                     IKNNRIAARYSGLKRLLSPQPAP"
BASE COUNT          315 a          532 c          667 g          336 t
ORIGIN      
        1 gtggttcggc atctgggcaa ggcgctgcag ccgcgggagg tgcgtttcgc tcgggagctt
       61 ccgaagacgc ggaacggcaa gatcatgcga agggtgatca gggctgccta cctgggaaag
      121 gaggagatgg gggatctctc gtcgctggag aaccccgcgg ccgtagagga gatagcgagg
      181 gcagcttgag ggaagtgggc catgccggac gatgttgcca tagcgggggt ggtcaggacc
      241 cccatcggct cgtttcgggg gccaaccgag gtgccggccc gacaattggc ggggcggaga
      301 agccggcgcg gtaggtgggg gtactgttgt gccggagaag gtggttcggt gctggcatcg
      361 gacgcatccg gcttgtagaa tggagaagct gcagcaggcg agtctggaat gatgacgctc
      421 ctggaccgtg agcgaggccg ctggcgggag tcgtcgaggg agggggagat gggagggttt
      481 tccaggggat gggttttcgg cgctgtaacc gggatcgctg ccgtatccgc ctataggatg
      541 gcgattcggc cctggcacct tcgatggggc gcgacggaga cagaggctag ctggcagctg
      601 ccgggcgacg aactggtgcc ggagccgaag atcaatgcga cccatgccgt taccatcgat
      661 gcgccgccgg aggcagtgtg gccctggatt gcgcagttgg ggcagggacg aggcggcttc
      721 tacagctacg actggctgga gaacctcatg gggctcgaca tccacagtgc cgacagcgtg
      781 atccctgagc accagcagat caaggagggc gacggcatcc cgctggcccc gggcggcttc
      841 gactttccgg tggtgaaggt ggagccccag cggatgctgc tcctgcacgg cgatacgcgc
      901 gacggcagct cgcccgtgcc cgtgaagccc ggggagtatt tgaacgcgag ctggctgttc
      961 ctgctggatg ccgccggcag aggtggaacc agactggtcg agcgcttcag ggcggactac
     1021 gatccgggtc tggtgaaggg gttggtgtac ggcgtcttcg tggagcccgg cgccttcctg
     1081 atggagcgga agatgctgct cgggatcaaa gagcgggccg agcagctggc ctaatcgtag
     1141 ggcggggtct tgtgccccgc cgtcagcgtg atcggaatcc accgacgagg gcgcaaccag
     1201 ggtgtgggag gcggggcaca agactccgcc ctacgccctt gccagtcggt gtcattttcc
     1261 ctcaccacgt gggtgttgca tcgcagcccc agccggggtt acggcgcggg ctgcggggac
     1321 agcagccgct tgaggccgct gtagcgggcc gcaatccggt tattcttgat ggccatggcc
     1381 agcgccctct tggtccccac cagcaccacc aactccttgg cgcgggttat ggccgtgtag
     1441 atcaggtttc tctgcagcag caggtagtgc tgggtgagca cgggtatgac caccgcgggg
     1501 tactccgcac cctgggcctt gtggaccgat atggcgaagg cgtggaccag ttcgtccaac
     1561 tccgagaact cgtactccac cagccggtcg tcgtcgaatg ccaccaggac caactgctcc
     1621 tccaggtcga tggccgtgat ccgccccgcg tcgccgttgt acacctgctt atcgtagttg
     1681 ttgcggatct gcatcacctt gtcgcccaac cggaacagct tgccgccgaa tcgcttctcc
     1741 ggcttatcct gatggggcgg gttcagcttc ttctgcagct cctcgttcag gaagtccacc
     1801 cccaccttac cacggtacat ggggctcaga acctggatcc cggagaccgg
//
LOCUS       DATALS010000406         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_336934, whole genome shotgun sequence.
ACCESSION   DATALS010000406 DATALS010000000
VERSION     DATALS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336934"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            540..2105
                     /locus_tag="VHS06_09065"
     CDS             540..2105
                     /locus_tag="VHS06_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988306.1"
                     /translation="MTGPMLKRLIRVSIVAAIATTMLGFTVGIAAADPGDHGRGHGNH
                     NAHGQVEKQSHNSGHGWNRDNDYSLRDDEDSNDDSQDDQGDRDENSRPGWGWGDRNHE
                     HFGPPGQGCDVEDECEEGRGAPSDVSTLAATHFAVSAPSSASTGTAFNFTVRALDQNN
                     DRTDNYDGTVHFTTSDGSASLPADAKLSNGVGTFSAMLRTTGNQTITAADTGNSSIAG
                     TSGTIVVSSGATTHFVVTAPSSATSGSAFSFTVRALDANNRRVFNYSGTVHFTSSDGS
                     ASLPSDATLSNGRGSFTATLRTTGNQNITATDTSNSSIAGTSGNIAVSSTTSTHLALS
                     SVPSTATAGSAFTVTVTAQDQNDNTVTGFGDTIHFTSSDGSAVLPNDATLSNGTATFS
                     VTLKTAGSQTISVSDTSNSAVAGASASVTVSPAGATHLAVLAPNSATSGTAFNFTVTA
                     LDQFNNTATGYTGTVHFTSNDSAAVLPADSTLTNGTKAFSATLKTAGSRAITTTDKDN
                     ASITGTSATIAVS"
     gene            complement(2335..>2629)
                     /locus_tag="VHS06_09070"
     CDS             complement(2335..>2629)
                     /locus_tag="VHS06_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX2988307.1"
                     /translation="EKERRKMQNALAKQSRRFHARLLESAYPTPSVLQLMMFRMGRTS
                     AKLLADDDNLDHTYYRNQGWFDSDYFYPAKLGPLKKAVGAAFDWTAAHMAKQR"
BASE COUNT          559 a          835 c          769 g          466 t
ORIGIN      
        1 aacgggaggc tgcactaaac gccttcaatc ggcgatttag gccaagcgag cccgacctac
       61 caatgaagcg gatagagcgt tgcatggcgg tcgagatcac aatcaccaag atgactggcc
      121 gaagggaaag ggcccgccat cgcgcatatt ggcgctccac cttctagccc ccttcgcgtc
      181 tggcaacgct tagatatctt tgagtcctca gcctttgccc cgtagcacag gacggctcag
      241 gcatcagttg cagcgtggta gtagtggtga aggatgtcgc cacagaaacc tgtacgggtg
      301 ccctaacatc gctgctacct ctcactaagt gcgtcgttcc tcaaatcagg aaccactctt
      361 gcagtaagtt gttgtaggag gtggttccga gggcagcata gtcgtgaacc tggacgaatt
      421 gggtcccgtg gaggaacgaa gcatttagaa cttgtaactt ccacaaagtt ggtccgttat
      481 atacaatgga ctttgcagct atggcgtaac ccccattccg caatggaaag gagcgcatca
      541 tgaccggacc catgctaaag cggctaatac gggtgagcat cgtggctgct atcgcgacga
      601 cgatgcttgg gttcaccgta gggatcgcgg cggccgaccc gggcgaccac ggccgaggac
      661 acggcaacca caacgctcac ggccaggtcg aaaaacagag ccacaactct gggcatggtt
      721 ggaaccggga caacgactac tccctacgtg atgacgagga cagcaacgac gactctcagg
      781 acgatcaggg cgaccgcgac gagaactccc ggcccggctg gggctggggg gaccgcaacc
      841 atgaacactt cggccctcca gggcagggct gcgacgtcga ggacgagtgc gaagaaggca
      901 ggggggcacc gtccgacgtg tccacgttag cggccacgca cttcgccgtg tcggcgccat
      961 cgagcgcgag cacgggtacg gcattcaact tcaccgtgcg ggcgctggac cagaacaacg
     1021 acaggaccga caactacgac ggcacggtcc atttcaccac ctcggatggc tcagcctctc
     1081 tgcccgccga tgccaagctc agcaacggag tcggcacatt ctccgcaatg ctccgcacga
     1141 cgggcaacca gaccatcacc gcggcggata ccggcaactc ctcgattgcc gggacgtccg
     1201 gcaccatcgt ggtgtcttcg ggtgccacca cccacttcgt ggtgacggct ccgtcgagcg
     1261 caacctcggg ctcggccttc agtttcacgg tgagggcgct ggacgcgaac aaccgcaggg
     1321 tcttcaacta tagcggcacg gtccatttca ccagttccga cggttcggcc tctctgccct
     1381 ccgatgctac gctcagcaac gggaggggaa gcttcaccgc cacattgagg acgaccggca
     1441 accagaacat caccgcgacc gatacctcca actcctcgat agcgggaacg tccggcaaca
     1501 tcgcggtatc ctcgacaact agcacgcatc tagcgctgtc gtcagtgccg tcgaccgcga
     1561 ccgccggcag cgcctttacc gtgacggtga cggcgcagga ccagaatgac aatacggtca
     1621 ccggcttcgg cgacaccatc cacttcacga gctcggatgg atcggccgtg ttgcccaatg
     1681 atgcgacact gagcaacggg acagcgacct tctccgtcac attgaagacg gcagggagtc
     1741 agacgatctc cgtgagcgac acgtcgaact ccgcggtagc gggggcgtcc gcctcggtca
     1801 cagtgtcacc ggctggggct acccacctcg cggtattagc cccgaacagc gcgacctcgg
     1861 gcacagcctt caatttcacg gtgacggcgc tcgaccagtt caataacacc gccaccggtt
     1921 acaccggcac ggtgcacttc accagcaacg attctgcggc ggttctgccc gccgactcta
     1981 cgctgactaa cgggacaaag gccttctcgg cgacgttgaa gacggctggg agccgcgcca
     2041 tcaccaccac ggataaggac aacgcctcga tcacgggcac gtccgccacg atcgcagtgt
     2101 cctgagacag gggcgccgcc agcaggcgca cgtgcgaaag caagacagac acactccgag
     2161 cttccagggt tggcctggaa gctcggagtg tgtctgtctt gttgtccgct tcccttgaag
     2221 agagccacct ctctgctgga gagtcaagcc agactacctg cccagacacc ttgtttgtca
     2281 gatcttgtta gcagcatcac tccccggcac gagggggcgg ccgctgccgg ttagctacct
     2341 ctgcttggcc atgtgcgccg ccgtccagtc gaaggccgct ccaacagcct tcttgagcgg
     2401 gccaagtttg gcggggtaga agtagtcgga gtcgaaccaa ccctggtttc ggtagtaggt
     2461 atggtctaga ttgtcatcat cggcaaggag cttcgcactc gtgcgcccca tcctgaacat
     2521 catgagctgc aagacggacg gcgtgggata ggccgactca agaagccgcg cgtggaacct
     2581 cctgctctgc tttgcgaggg cgttctgcat cttccgccgc tccttctcc
//
LOCUS       DATALS010000407         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_426969, whole genome shotgun sequence.
ACCESSION   DATALS010000407 DATALS010000000
VERSION     DATALS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426969"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..713)
                     /gene="ccsA"
                     /locus_tag="VHS06_09075"
     CDS             complement(48..713)
                     /gene="ccsA"
                     /locus_tag="VHS06_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX2988308.1"
                     /translation="MSVFWRVICGLGISAVIVAAFAFVPPAQGFRDPEGARIIFFHVP
                     MAWLAVLGFLTAMVNAVRYLRTRRLEYDVRSVAGAEMGFLFCLLATVSGAIFAKIQWG
                     TAWNWDPRETSIFVLLLIYGAYFALRSSVADDERRAALSAAYASLAVFPMLFLVFVLP
                     RMEPGLHPSDTVRSGHMSPEYRAVFFAALFCFTLLFAWVYQLRTAVGRMGGRAAVAWR
                     EGL"
     gene            complement(770..1453)
                     /locus_tag="VHS06_09080"
     CDS             complement(770..1453)
                     /locus_tag="VHS06_09080"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX2988309.1"
                     /translation="METASCGWAAESVAVLRKDLLSELRSRQALNAMVIFAVTTLAVV
                     SFALGPHALTARAQSALLWVILFFAAAASLSRPFLREEEGRTSTALRLSAVPAHVFAG
                     KLAFNILLLLGLDALLVPLYLGVMQAPVGSPEVLLMVVLLGSLGLAAACTIVAAIISR
                     ATTPGALFGGLAFPLLLPLLLAAVGGTTKAFEGAAVAAAWPELRLLLSFTVVVVTASW
                     MLFEVIWDD"
     gene            complement(1422..>1625)
                     /locus_tag="VHS06_09085"
     CDS             complement(1422..>1625)
                     /locus_tag="VHS06_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX2988310.1"
                     /translation="QRLKYAFALLHRPAVLFLDEPTANLDDAGIAFARQVIARQRADG
                     ILVIATNDREELEYGDRVLRLGG"
BASE COUNT          381 a          538 c          496 g          210 t
ORIGIN      
        1 cagccagatc aacagcacca cacccatcat ccagtagaga gacacgacta gaggccctcc
       61 cgccacgcca cggccgcgcg gccacccatg cggccgacgg cagtcctgag ctgatagacc
      121 caggcgaaaa gaagggtgaa gcaaaacagg gcagcaaaga acacagcacg gtactcaggg
      181 ctcatgtgcc ccgaacgaac cgtgtcgctc ggatgcaggc ccggttccat ccgcggaagc
      241 acgaacacca ggaacagcat gggaaacacg gccaggctgg cgtacgcggc gctcagcgcg
      301 gcgcgacgct cgtcgtcggc taccgaagac ctgagcgcaa agtacgcgcc gtagatcagg
      361 agcagcacga agatggacgt ctcgcgtgga tcccagttcc aggcagttcc ccattgaatt
      421 ttggcaaaaa tggcgccgct gacagtggcc agcagacaga aaagaaatcc catctccgca
      481 ccggcgaccg acctaacgtc atactccagg cgcctggttc gcaggtagcg aacggcgttc
      541 accatggccg tgaggaagcc cagcacagcc agccaggcca taggcacgtg aaagaagatg
      601 atgcgtgctc cctcgggatc ccgaaatccc tgggccgggg gcacaaaggc aaacgcggcc
      661 acgataaccg cacttatccc gagcccacag ataaccctcc agaagacgga caaatcacgc
      721 tcctcctcgt cgaggtcggg cgcccggggc aggggtcgct ccagatactt cagtcatccc
      781 agattacctc gaaaagcatc caggacgcgg tgactaccac cacggtgaaa gagagcagca
      841 gccttagctc gggccaggca gcggcaaccg cggctccctc gaacgctttg gtggtgcctc
      901 ccaccgccgc caggagaagc ggtaggagca aggggaaggc aagcccgccg aaaagcgccc
      961 ccggggtggt cgcccgggag atgatcgccg ccacgatggt acaggccgct gctaggccca
     1021 gactccccag aagcaccacc atgaggagga cttccggact gcctaccggc gcctgcatca
     1081 cccccagata gagcggcacc aggagcgcgt ccagacccaa aagtagcaga atgttgaatg
     1141 ccagcttgcc cgcgaagacg tgggcgggga cagcggagag ccgcagcgcg gtcgatgtcc
     1201 gtccttcctc ctcgcgcagg aagggccgag aaaggcttgc agcggcggca aagaatagga
     1261 tgacccacag gagagcggat tgtgccctgg ccgtgagcgc atgaggcccc agggcaaagc
     1321 taacgaccgc cagtgtggtc accgcgaaga tgaccatggc gttcagcgcc tgccgactcc
     1381 gcagctcgga cagcaggtcc ttacgcagta ccgcgacgct ctcagccgcc cagccgcaag
     1441 acgcggtctc catactccag ctcttcacgg tcattcgtag caatcaccag aatcccgtcg
     1501 gcacgctgcc tcgcgatcac ctgccgcgcg aacgcgatgc cggcgtcatc caggttggcg
     1561 gtcggctcgt ccaggaagag aacggccggc cggtgcaaca gggcgaacgc gtacttgagc
     1621 ctctg
//
LOCUS       DATALS010000408         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_402132, whole genome shotgun sequence.
ACCESSION   DATALS010000408 DATALS010000000
VERSION     DATALS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402132"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..1194)
                     /locus_tag="VHS06_09090"
     CDS             complement(310..1194)
                     /locus_tag="VHS06_09090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2988311.1"
                     /translation="MDSYLIDVSLSRLRLQFDISEVTEGLLLWRETVLPLLFSDSSIG
                     SAWQQENLSQLDEYIQHLVSRFGHLYAEAVHQKLETQRRRTAVMEDRQRLARELHDSV
                     TQSLYRIALFAEAAAEQLAEGNPDRTKHHLQLLRTTAQDALREMRLLIFEAHPPVLEK
                     EGLTGALRARLEAVETRGGLRTALLVEGVMRLPLSVEEELYHIAQEALNNVLKHARAQ
                     QVTVGLRMDEASIRIVVADDGDGFIPADVLGKGGMGLQGIQERAQRLGAKLSIDSTPE
                     KGTRLAIEMPAEDPGVWP"
     gene            1762..2289
                     /gene="pyrR"
                     /locus_tag="VHS06_09095"
     CDS             1762..2289
                     /gene="pyrR"
                     /locus_tag="VHS06_09095"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX2988312.1"
                     /translation="MDSKTIMTADDVRRVLTRVSHEIVEWNQGADGLVLVGLRTRGIP
                     LAERLGARIEEYEKSSVPVGKLDITLYRDDVDTRGPLPIGPTDIPVDITGKRIVLVDD
                     VLYTGRTIRAAMDAMIDYGRPAMIRLMVLVDRGHRELPIRADFVGKNLPTSRDEDVIV
                     HLMEVDGVDEVLLTR"
     gene            2306..>3339
                     /locus_tag="VHS06_09100"
     CDS             2306..>3339
                     /locus_tag="VHS06_09100"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX2988313.1"
                     /translation="MVETTVDVLEKPGTDGVVPLDQVAREPFRRRHVLDLDDFSAGEI
                     LKVLDTADIMKDILRRPIKKVPTLRGKTVVTLFYEASTRTRVSFETAAKNLGADTVSV
                     AATTSSVSKGESLVDTLCTLQALGADAVVMRHSYSGAPYLASRILKGSVINGGDGWHA
                     HPTQGLLDVYTVREKLGRVSGLKVVILGDVLHSRVARSAIWSMTKLGAEVTVCGPATL
                     LPSWWVQTAAQNDGQVMPGLRLSCDLDRTVEGADVVMALRLQLERMQAGLLPSKREYI
                     RMFQLNSERVSRAKPGALVMHPGPMNEGIEIAPDVAHGDQSVVEAQVSNGVAIRMALL
                     YLLIGPNATVP"
BASE COUNT          592 a          987 c         1085 g          675 t
ORIGIN      
        1 gaatacgccg ccgcggatta gtcaactgac acgctggatt tgaccgctag gagcccgggt
       61 cgtccctgac ccgggcggtg gcgtgagagg ccgtcggggt cagggacgac cccgactcct
      121 agtgtcaaga tcagcgtgtc aaagggctag ggtcctcctg tcccatattc atcactccaa
      181 cgtaccgcca gagggcggcc acgtcggggg gggacgagga ccactgggtc cccggagtgc
      241 aggtcggctc cgtgcccctg cgaacgtcag cgtgatcgtc cacgaccagc acccggattg
      301 cgttccggct catggccaca cccccggatc ctctgccggc atctctatag ctagcctggt
      361 tcccttttcg ggtgtgctgt cgatgctcag cttcgctccc agccgctgtg ctcgctcttg
      421 aatcccctgc aaccccatgc cgcccttccc gagtacatcc gcggggatga acccgtcacc
      481 atcgtctgct accacgattc ggatcgatgc ctcgtccatc ctcagtccga cagtcacctg
      541 ctgagccctg gcgtgcttca gcacgttgtt cagcgcttcc tgggctatgt ggtagagctc
      601 ttcctccacg ctcaggggca gcctcatgac accttcgacc agcagcgcgg tacggagtcc
      661 acctcgcgtc tcgacggcct ccaatcgagc gcgcagcgct cctgtcaggc cttccttttc
      721 cagcaccggt gggtgcgcct cgaagatcag cagccgcatc tctcgcagcg cgtcctgggc
      781 cgtggtgcgc agtagttgca ggtggtgttt ggtcctgtcc gggtttccct cggccagttg
      841 ctcggcggca gcctcggcga agagggctat tctgtagaga gactgggtta ccgaatcgtg
      901 gagttctcgg gccagccgct gccggtcttc catcaccgcg gttcgacgcc gctgcgtttc
      961 cagcttctgg tggacggcct cggcatagag gtggccgaag cgggatacca ggtgctgaat
     1021 gtactcgtcc agttgggata ggttctcctg ttgccatgcg gaaccgatcg aggagtctga
     1081 aaaaagcagg ggaaggaccg tctcccgcca gagcagcagc ccctcagtca cctcggagat
     1141 gtcgaactgc aagcggaggc gactgaggct gacgtcgatg aggtagctgt ccatggcggc
     1201 gtaggagccg gtgcgcagcg cctccaggaa ggaatcgagg cagcgcagtg ccgcatcgtg
     1261 cagctcttcc gtcggaagct cgacgtagtg ggtgccgggg gatgcggcag accctatcag
     1321 cccatccggc agctatctcg tggcgatggc tgttcagcaa ggtcgccaga tcgatggcct
     1381 gctgtcctct ggccgtcatc acctcaccct ccacgatgcc tatacaggtg ggcccgagag
     1441 agcgtcggta gctttcctct agcggctgtc tgatgtgacc ccttcatggg atcgaccgaa
     1501 ggctttggac tattggatta tagcgggcgg cggtcgcact tcaaagatgt gattgtctcg
     1561 tgaggttgag ttctccctcc tcttctggta tagtaaccgt cagttgatgg ctatcctcta
     1621 gggagagttg gcccaacgcc tttaatccgg tcctgcgagg ccgggaagga gaaagcttta
     1681 tgtggtcttg cggcgtgaaa acccctgcgc agccgagagc ggtgcgaagg ggcttttttg
     1741 tgcccattga gggagggaga cgtggatagc aaaacgatca tgacggcgga cgacgttcgc
     1801 agggtcctca cccgggtttc ccacgagatc gtggagtgga accagggtgc ggacgggttg
     1861 gtactggtgg ggttgcgaac caggggcata ccgctggcgg agcggctcgg ggcgcgtatc
     1921 gaggagtacg agaagtcgtc cgttcccgtc ggtaagctgg acataaccct ctatcgcgac
     1981 gacgtcgata ctaggggacc tctccccatc ggccccaccg acatccccgt ggacatcacg
     2041 ggcaagcgga tagtgctggt ggacgacgtt ctctacacgg ggcgcaccat tcgggctgcc
     2101 atggatgcga tgatcgatta cgggcggccg gcgatgatcc ggctgatggt attggtcgat
     2161 cgtggccata gggagttgcc gatacgagcc gattttgtag gaaagaactt gccaacctcc
     2221 agggatgagg acgtgatcgt ccacctgatg gaggtcgacg gcgttgacga agtgttgctg
     2281 actcggtgaa gaaaggaggt tggcgatggt agagactaca gtagatgtat tggagaagcc
     2341 cgggactgac ggcgtggttc ctctggacca ggtggcacga gagccgttcc gtcgccggca
     2401 cgtactggat ctggacgatt tctccgctgg tgagatcctc aaggtgttgg atacggccga
     2461 catcatgaag gatatcctcc ggcgtcccat aaagaaggtg cccactctcc ggggcaagac
     2521 ggtggtgacc ctcttctacg aggccagcac ccggacgcgt gtctccttcg agactgcggc
     2581 gaagaacctg ggcgccgaca cggtgagcgt ggccgccacc accagcagtg tgtccaaggg
     2641 agagtcgctg gtagacacct tgtgcacgct gcaggctctc ggcgcggatg ccgtcgtaat
     2701 gcgtcattcc tactccggag ccccctatct ggcttccagg atcctcaagg gctcggtgat
     2761 caatggtggg gacggctggc atgcccatcc cactcagggc ctgctggacg tctacacggt
     2821 tcgggagaag cttggccgag tatcgggact gaaggtggtg atcctgggcg acgtcctgca
     2881 cagccgtgtt gcccgatcgg ctatctggtc aatgaccaag ctaggtgccg aggtgacggt
     2941 gtgtggccct gccaccctgc tcccctcatg gtgggttcag accgccgccc agaacgacgg
     3001 gcaggtgatg cccgggctgc ggctctcctg cgacctggac cgaaccgtcg agggggcgga
     3061 cgtcgtgatg gcactccgcc ttcagctcga gcgtatgcag gcaggcttgc tgcccagcaa
     3121 gcgcgagtac attcgcatgt tccaattgaa ctccgagcgg gtatccaggg ccaagccggg
     3181 cgcgctggtt atgcaccccg gtccaatgaa cgaggggatc gagatcgccc cggacgttgc
     3241 tcacggcgat cagtcggtgg tggaggccca ggtatccaac ggtgtggcta tcaggatggc
     3301 gttgctctac ctgttgatag ggcccaacgc gaccgttcc
//
LOCUS       DATALS010000409         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_427010, whole genome shotgun sequence.
ACCESSION   DATALS010000409 DATALS010000000
VERSION     DATALS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427010"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VHS06_09105"
     CDS             complement(<1..518)
                     /locus_tag="VHS06_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988314.1"
                     /translation="MGRTCSRALAAALALALAGFATACTPPPPPPATVQSVPIAVTLP
                     PVAQSAPRSWIDAPLHGSSIALGPVQVVSHSTHPGGVSEVELSVDGNIVDSEVSPNPK
                     KALVTVRQDWVPKESGKRVIRLRARNGTGDWGAYATAEVTIMAGPTEIPIVPSPQPTA
                     GPTLSGTATPTAT"
     gene            complement(890..2329)
                     /locus_tag="VHS06_09110"
     CDS             complement(890..2329)
                     /locus_tag="VHS06_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX2988315.1"
                     /translation="MANQSAVAKKSAVSQGFLDSFFKISESGSTVNTEIVAGITTFMV
                     MAYIIFVNPAILSFSGIKDLQGLGPAFPAVMAGTCLVAGIMTIFMGTYANYPFALAPG
                     MGLNAVVAFQLIVGMKLPWQAAMGVIFLEGIAITILVITGLREAMFRAIPLSLKNAIG
                     VGIGLFIFFIGLNDGELIKWGSKDIGTPVALGNLTTGPVLVALFGLFLTIWLMAKRVK
                     GALLFGILLSTVFAIIVNYASGLKAFTTPGAAVMPTSLVAVPDLSLLGQGINFGIFAK
                     VGFLTAILTIFSLMLSDFFDTMGTVIGIGGKVGWLDKNGGLPRLNRVLLVDSLAAVFG
                     GAASTSSATTYVESAAGVGEGGRTGLTAVVVGVLFLLAMFFSPIAAVVPAHATAPALI
                     IVGFLMSSVAKEIPFSDLEEGLPALLTIASMPFTYSITNGIGVGFISYTFIKVVMGKS
                     AKLHWSMWVTSIAFVIYFALPWLNVTFGI"
     gene            3106..3633
                     /locus_tag="VHS06_09115"
     CDS             3106..3633
                     /locus_tag="VHS06_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX2988316.1"
                     /translation="MDGNLAQLAVPAWELALRCAVIYLGLLIGLRLFGKREVGQITLF
                     DLVTVLLVANAVQPAMTGPDSSLVGGLIIIATLLLLNRAMGWARLHSPLFRQLLQSHP
                     TVIAQEGHWLPNAMKREGLAEDEAEMALREHGLSSVKEAKLAVLELDGTISIVPTDSR
                     TIRTRRRLRLLRPRQ"
     gene            3638..>3909
                     /locus_tag="VHS06_09120"
     CDS             3638..>3909
                     /locus_tag="VHS06_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX2988317.1"
                     /translation="MEPPRYRHGPIGRYRQVVQILARHGFGWLLAQLGVGGRALIPWG
                     LFGRIQTPPLPTQAEQLRLAIEELGVTYIKLGQILSTRADLLPAEY"
BASE COUNT          757 a         1234 c         1243 g          675 t
ORIGIN      
        1 gtcgcggtcg gcgtcgcggt gcccgacagg gttggcccgg ctgtgggttg cggcgatgga
       61 acgatcggta tctcggtcgg cccggccatg atcgtaacct cggcggtagc gtaggcgccc
      121 cagtctccgg tgccgtttct ggcccgcagt ctgatgaccc gcttccccga ctctttggga
      181 acccagtctt gccgtaccgt caccagggct ttcttcggat tcgggctcac ctcggagtca
      241 acgatgttgc cgtccacgga cagctccacc tccgagactc cacccggatg ggtggagtgc
      301 gagaccacct gcacgggacc gagggcgatg ctgctgccgt gcagcggcgc gtcgatccag
      361 gagcgggggg cggattgcgc gaccggcgga agggtgactg ctatgggaac ggactgaaca
      421 gttgccggcg gtggcggggg cggcgtgcag gccgtggcaa accccgcgag ggcgagagcc
      481 agtgcagcag ccagggctcg actgcacgtt ctgcccaacc gaacccccga aggccgccct
      541 tcttcggcag ggtagtgctc gcgcgcggca tcacatatgc ccgcgctccg gggcacgcgg
      601 ggccgtcgca tcgcgcttac catgggatgt cctgcttgag ttggctggag gtgggcgggt
      661 ctatggaaca ggtcatccac tggttcttcg gcgggcagcc gatctccgcg taggctgcag
      721 cctgcgagcc ctggtacagc cggtaccagt cgtcgaagca gaccgcccct ccggcgtcct
      781 ggatcgctag agtggtagcg ccgacaacga acgaggggac aagccccgca ggacttgtcc
      841 cctcgcacgc tatcgaactg cttgcaggga tttcctaccc ccgtcccgac taaatgccga
      901 aggtgacgtt gagccagggt agcgcgaagt agatcacgaa agcgatggag gtgacccaca
      961 tcgaccagtg aagcttggcg ctcttgccca tgaccacctt gatgaaggtg taggagatga
     1021 agcccacacc gatgccattg gtgatgctgt aggtgaaggg cattgaagcg atggtcagca
     1081 gcgcgggcag cccctcctcg aggtcggaga aggggatctc cttggcaact gaggacatca
     1141 ggaagcccac gatgatcagg gcaggcgcgg tggcgtgggc cggaacgact gccgcgatag
     1201 gagagaagaa catcgccagc aggaacagca cgccgacgac cacggccgta agaccggtgc
     1261 gaccgccctc gccgacgccc gcggcgctct ccacgtaggt agtggcggag ctggtgctgg
     1321 cggcgccgcc gaagaccgcg gccagggagt cgaccagcag cacgcggttg agtcgcggta
     1381 ggccaccgtt cttatcgagc catccgacct tgccgccgat gccgatcacc gtgcccatgg
     1441 tgtcgaagaa gtccgagagc atcagggaga agatggtcag gatcgccgtg aggaaaccga
     1501 ccttggcgaa gatcccaaag ttgatcccct ggcccagcag ggagagatcc ggcacagcca
     1561 ccagggaggt cggcatgacc gccgcaccgg gggtggtgaa cgccttcaat ccgcttgcgt
     1621 agttgacgat gatggcgaag acggtggaga gcagaatacc gaacagcaga gcacccttca
     1681 ctcgcttggc catcagccag atggtgagga agaggccaaa gagtgcaacc aggaccggcc
     1741 cggtggtgag attgccgagg gcgaccgggg tcccgatgtc ctttgagccc cacttgatca
     1801 actcgccgtc gttgagaccg atgaagaaga tgaacaggcc gatgccgacg ccgatggcgt
     1861 tcttcaggga gagcgggatc gccctgaaca tcgcctctcg cagtccggtg atcaccagga
     1921 tggtgatcgc gataccttcg aggaagatga cgcccatagc agcctgccag ggcagcttca
     1981 tcccgacgat gagctggaag gcgacgaccg cattcagccc catgccggga gcgagggcga
     2041 agggatagtt ggcatacgtg cccatgaata tggtcatgat accggcgacc aggcaggtgc
     2101 ccgccataac cgcggggaac gcggggccca gaccctggag gtccttgatt ccagagaagg
     2161 acaggatcgc tggattaacg aagatgatgt aggccatcac catgaaggtg gtgatgccgg
     2221 ccacgatctc ggtgtttacc gtgctaccgc tctccgagat cttgaagaag ctatcgagaa
     2281 atccctggct tacggcagac ttctttgcca cagcggattg atttgccaca gcctcccctt
     2341 tcctttcttg ggcgcactag cacccaggag tgatgaatta gtgggacaat cgggtcgaag
     2401 aacggtgcac cccatgggca caccggcggc ccaaccacaa cctcgcgcgc caaccatggc
     2461 acaggactcg tgttacgtca agcacacctc cagcacgcat gcaccaccgg tgccgctgac
     2521 tgagcctaca gggccggctt ctccacgaat tgctgccccg tccaggctat tgtcctcact
     2581 gatcgccgcg gcaacccgcg gcactcgggg catgggtccc aaatgcgtga gcaacttcct
     2641 tggatgcgag gctgtctatc tgccgccccc ggcctagaaa accggcccaa ctatggacaa
     2701 acggccagat tcgattaggt caatccgtgc tgtgaagtgg tgaggttggt tagctgtacg
     2761 ttttccgatt cggctgccgc tccggggcga gcggcgggga gggacgttct gaaccgactc
     2821 ctccaccctg attagtgtac attgcgaaat tggttcccgc aacatttcta caccggtcac
     2881 gcagtgactg ataggtcatg ggcccagaaa tccacgcgcg gccgtgtcac agtcttgagg
     2941 gagagcctct gcgctcccag gggcttactc tccgcgtccc cacctccccg cgtccccgtg
     3001 tccccgtgtc tccgtgtccc accccccatc ccccatcccc caccccctat cgttggcact
     3061 gcacgtgcac gtcaatggtg aagaagatcg tcgggagggg tgcccatgga tgggaacctt
     3121 gcgcagctgg ccgtgccggc ctgggagcta gcacttcgct gcgcggtgat ctatctggga
     3181 ttactgatcg gcctgcggct gtttggcaag cgcgaggtcg gccagatcac cctcttcgat
     3241 ctcgtcacgg tgctgctcgt cgccaacgcc gtccagccgg ccatgaccgg gcccgacagc
     3301 tccctggtcg gtggactcat catcatcgca acattactgc tgctgaaccg cgcgatggga
     3361 tgggcacggc tgcacagccc cctcttcagg caactgctcc agagccaccc cacggttatc
     3421 gcgcaggagg gccactggct cccaaatgcg atgaagcgcg aggggctcgc cgaagatgag
     3481 gcggagatgg cgctgcgaga gcatggcctg tccagcgtaa aggaggcaaa gctggcagtg
     3541 ctggagctgg acgggaccat cagcatcgtt cccaccgact cacgcaccat tcgcacccgc
     3601 aggaggcttc gcctcctccg cccgcgacaa tagcccgatg gagcctccgc gttaccgcca
     3661 cgggccgatc ggtcgctacc gccaggtagt gcagatcctc gcgcgccacg gcttcgggtg
     3721 gctgctggcc caactgggcg tcggcggaag ggcactcata ccctggggat tgttcggccg
     3781 gatccagaca ccaccgctgc caacacaggc cgagcagctt cggttggcga tcgaggaact
     3841 gggagtcact tacatcaaac taggccagat cctctccact cgcgccgacc tcctccctgc
     3901 cgaatacat
//
LOCUS       DATALS010000410        10019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_180056, whole genome shotgun sequence.
ACCESSION   DATALS010000410 DATALS010000000
VERSION     DATALS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10019
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180056"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VHS06_09125"
     CDS             <1..538
                     /locus_tag="VHS06_09125"
                     /inference="COORDINATES: protein motif:HMM:NF019021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEX2988318.1"
                     /translation="LMQAWGYERVPGAKNWNWGPHQAVEGFVLDLRSIGVERWIESIL
                     SGEPMPRTFKPVDVESELQRLLPRWWDDELLTSSPLLRLPAVSPAARGTDRAGALRRA
                     VQEAVEAGAAIASPEMAESYRALDMAYMHRAGTHEQVAEQLCVSRATFYRLLKRAIRA
                     TSELLTRAPADPQTRRAS"
     gene            986..1384
                     /locus_tag="VHS06_09130"
     CDS             986..1384
                     /locus_tag="VHS06_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015847804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX2988319.1"
                     /translation="MQADANPQGTAGATSGMAPNLAAALSYLLGIITGVVFLMIEKNP
                     FVRFHAWQSILFSVAWIGFWIVFTVLSSILGMIPFLGFLVVLIGLLLSLVLGLGSLIL
                     VIVMIIKAYQGERYKLPFIGDMAEKYASGQ"
     gene            1455..2444
                     /locus_tag="VHS06_09135"
     CDS             1455..2444
                     /locus_tag="VHS06_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988320.1"
                     /translation="MKRLVVMVLVVLAVASAGCQQAAEKATQKAVEQATGVQVSQKGD
                     QVTVKGKDGESVTVGSQVSDELKNFPVPQGFKMDKDGLGTVSSKGDTLSVGEWEGSGT
                     PASVLEFYKKTMPGLGWTEDLSFSTETGGQVSYTKGQSQAVVTINKSDSTTQLSIMVG
                     KSGSLKSNSSSAGGSGAGASSGTNIATTGSTPAPAAAPTKAAAATPKPEATATPAAPK
                     LGSASSVPDELKQVPLPSGFSVVDGSMMRQAAGGVFALATGKWFGKSSVADVVKFYKT
                     AMANDWDEDYVTTSGDEAICTYTNKKKPELSAEIEVMKEEAGTIVTVSLTQDN"
     gene            2826..3770
                     /locus_tag="VHS06_09140"
     CDS             2826..3770
                     /locus_tag="VHS06_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988321.1"
                     /translation="MRDSAVLALLLLAMLVVVGCGESAHATGVNDGPSVGQTDITRST
                     GEPSAVGPQYPDALKKFPVPQGFALGKGGHATVMQRGDLVAVATWKGSGSISGVVEYY
                     RQSLPKQGWGENVTFSSDQSAQFTYTKDLCQATTLVEKDGNDIVISVAIGRTSEDIPT
                     PVPTATPVPTPTRVPTATPKGAVGRNVPPTVVPLATFTPQPLSRGDASTVPAELKDVP
                     VPGGFTLVDGSARRVAQGGVLGMAAADWFGKSSPKEIGAYYRSALAKGWNDDSFVESA
                     NEVNAQYTSKKDARLTLLIEVSLFESDTVIHMSLVRSQ"
     gene            complement(3880..4746)
                     /locus_tag="VHS06_09145"
     CDS             complement(3880..4746)
                     /locus_tag="VHS06_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988322.1"
                     /translation="MSERIRSLRHGLVAIAMAVVAGCASSTPSFPLAETPAPPPEATL
                     RPVPSSTAAPAAAATATIPAAAGQAMPIPTVVAAPSMGRFAAVEGPPEVEQTLRAAVE
                     LQRRMESPAAGSPSRDDVVWMYQRTAELMETVSLQLPQMVPGQRDQAMKRMDELINGM
                     VRVLSSNIQTTNEQGPTVAFGTPMAVQGTPLVVVGTPRALTGLNRPAPADLLAHDIEQ
                     TQSRAVELSQGKPSGDELVVLTTSLGATATTVRQLSGYLATEDLQRLTDGMAGALRAL
                     ANLAANYSTTTA"
     gene            4942..6192
                     /locus_tag="VHS06_09150"
     CDS             4942..6192
                     /locus_tag="VHS06_09150"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX2988323.1"
                     /translation="MRDTVPEFNISVPITGNIWIVGIVFLLHITAVAFIIGIAMIAPV
                     AELLGMRTGGERWERLAYQLSTIIERIFAWGATWAAFSLVAIWGLFPRVWGYLSSTFF
                     IQIIIIAGILWFVMSVTAYLYYVTWEPLRQRRLLHNAIGWTFVLFTMIFITTIVMFSS
                     YQLTPTDGGDRMLTAAANPSYGTEAAHRHIGNLSYGGILLAGLLGGWLLLFGAGGEGD
                     RRSFRSWAADAGFSVGAVVLLLMPFSGWFYANQIRLASPGAFALMMLGDNGWMFRVQI
                     GLLGTAFLLANQYIYLNVPRDRINAGQLSWIRYSLWVNVLLIGLAVLPKDVPLGQMRP
                     WKYIALAGFILLLLINLTTYLRSRRGAAWVTSGRWSHAVLVALGIVVVALFVMMGIIR
                     TSARGNWLIYERMPVDQGQELVRP"
     gene            6206..6424
                     /locus_tag="VHS06_09155"
     CDS             6206..6424
                     /locus_tag="VHS06_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988324.1"
                     /translation="MKTEAAIIFVIEIAGFVFFLGLLPLAVVVGRLTRVWRRDRGVGL
                     LLLGVLINTAFLFFTLFAAFAGLVLYGG"
     gene            6429..6950
                     /locus_tag="VHS06_09160"
     CDS             6429..6950
                     /locus_tag="VHS06_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX2988325.1"
                     /translation="MSQPGVAVPSSSRRRFLHQLGIAVVGGIVSLLTAVPLVGYFFSV
                     LTKKPSLQWVRLCSLDQVDSEQPREFRITFPGENSSVPYQAVQGVFVLRHGDQILVFS
                     NVCTHMGCSVRWLAWRQQIICPCHGGMYDRWGHLMGGPPSSDLPLYLSRIEDNDLYVA
                     NRIIQRGVTTEGG"
     gene            6955..8148
                     /locus_tag="VHS06_09165"
     CDS             6955..8148
                     /locus_tag="VHS06_09165"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEX2988326.1"
                     /translation="MFGGVIDVVYGWLDRRLPFTRTLNRVLKDPVPERGGWWYTLGAA
                     IFVLLTIQIITGIFLMFYYVPSWNEARDSILYIQQQVFLGWLVRGIHYWNQVALVFLV
                     GAHMARTFFSAAYKAPRELIWVLGVTLLLLMVGTAFTGGILRWDQSGYFDVVVGTTIA
                     GWTPIIGPGLAQLWRGGDTITPLTLTRTFVFHVWLLPAPLFLLAVIHVGLVVVQGQYG
                     SWVNYEQEPPDAHPMDEDEIASREKAKRELLDPESHKVNLPVRTTWFFPYHVFKEGIV
                     SGVMFLGVFLAACFFAVPVEEAVDPSTTTYAPSSMWFWLFLDQMFLIFPGAWMIPFGV
                     IAAPAIIVTIFYLLPWLDRNPGLKPYHRPVAVTFMVVIIMSILVLAMLGASRVYNYDF
                     VNTPG"
     gene            8285..9616
                     /locus_tag="VHS06_09170"
     CDS             8285..9616
                     /locus_tag="VHS06_09170"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX2988327.1"
                     /translation="MEPTIVPEVARRFTIGFLFVTHIQFAAFLIGLFSLAVSFEFFSL
                     LNRGDENLDRLAHGLAKTAAYMYSTGAVLAFMVMFFATIFWPTFWYTIIRINFWPFFL
                     EAITFALTILYLFPWYFTWHRLANFRWVHLSLGAALIVSVYFQQSLIDVVAAYMLTSV
                     PPALFLRVFFNPTVIPLDMHRMVGDISFAGFVVAGYGAFRTLRAREAKERGYYDWMGS
                     LGLIAGLAFMFLQPAIGIEYLQEIRSASPGAFSVMMRGHNSWLFLLQVLFLSILFVAS
                     MVYILLQVRKSGTSGVRWLTGLLIVAILSALLLVQPRVIGPSQEYMWVNWVNPIGAMQ
                     PWKYIAFAGMTLSGIAAVAIYTGKYRRGLRWGYLERGGRGSQYVLLGLALFASGMMAL
                     MGYIRENSRIPYLIYYQQRLDQPERFPELRPTPTPGLGGFGVEGQPEGGAQ"
     gene            9613..>10019
                     /locus_tag="VHS06_09175"
     CDS             9613..>10019
                     /locus_tag="VHS06_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988328.1"
                     /translation="MKTCLSLPGIRKWSLIAFLAFLSPVATSCGVVYVPLQPYPLIGE
                     GPPPGYTGGTVPERYSAMRDPFLPTDQTALQAGKSLYASHEPSCATCHGDRGHGDGPL
                     AAYLEPQPADFTAPPMLNAFRNHQPYVYWWVSEG"
BASE COUNT         1756 a         2971 c         3099 g         2193 t
ORIGIN      
        1 gctcatgcag gcctgggggt acgagcgcgt gcccggcgcc aagaactgga actggggacc
       61 ccaccaagcc gttgagggct tcgttctgga cttgaggagt atcggcgtgg agcgatggat
      121 cgagtccatc ctgagcggcg agccgatgcc aaggacgttc aagcctgttg acgtggagtc
      181 ggagttacag aggctgcttc cccgttggtg ggacgacgaa ctgctcacat catcgccact
      241 cctgcggcta ccggccgttt cccctgccgc gcgcgggacc gaccgcgcgg gggcattgcg
      301 aagggcagtg caggaggctg ttgaggcagg tgcggccatc gcatcccccg aaatggccga
      361 gtcatatcga gcgctggaca tggcctatat gcacagggct ggaactcacg agcaagtcgc
      421 ggaacagctc tgtgtatccc gggccacgtt ctatcgactc ctcaagcggg caatccgtgc
      481 tacgtcggaa ttactcaccc gtgcccctgc tgatccgcag acgcgacggg cttcctgagc
      541 ccttcccctc actttggctg aagctgtagc ttcgttgggc cccacacctg gtcctcaaga
      601 tgtggttgat actgcattga gacctcagtc ttcccgttct cctctccgta gactcactct
      661 tgcctcgtga ggacagagtg ccatgctcga ggaccggctt tccttgcgtg acgggcggtt
      721 gccgcccacc ggctctctgt cgacacggtg ctctggataa tcatcacctg ggtgcggcct
      781 gagaggccga taccattctc tggaactaag ttgggattgc ttggcgctat ttcaggctga
      841 atggggtggg cgcaggcaaa gtccatccgg aaggcgttgt ggaccgtctc aggttgagac
      901 gagcgtgaga ctgccttggg atcatgtcgg atttagacta taccgcggcc tactcatgcc
      961 catatcacat tgtggagagg aacccatgca agccgatgcc aacccgcagg gcaccgcggg
     1021 cgcgacttcc ggaatggcgc caaacctggc tgccgctctc agctaccttc ttggcataat
     1081 caccggcgtt gtctttctca tgattgagaa gaatcccttc gttcgtttcc atgcatggca
     1141 atccatcctg ttctcggtgg cgtggatcgg attctggatc gtcttcaccg ttctgtcgtc
     1201 gatcctgggt atgatcccgt tccttggctt cctggttgta ctgattggcc ttctgttgag
     1261 ccttgtcctg ggcttgggga gtctaatcct cgtcatcgtg atgatcatca aggcatacca
     1321 gggcgagcga tacaagttgc cattcatcgg cgacatggca gagaagtacg catccgggca
     1381 gtagccccgc acgatcattt ctggttgacc acggcatttt cacagtccga catggttgga
     1441 aatgggaggt aggattgaag cgtctagttg tcatggtcct ggtagttctg gcggttgctt
     1501 ctgccggctg ccagcaagcg gcggagaagg ccactcagaa ggcggtggag caagccactg
     1561 gggtgcaggt aagccagaag ggtgaccagg ttaccgtgaa gggcaaggac ggtgagtcgg
     1621 tcaccgttgg atcccaagta tcagatgagc tgaagaactt ccccgtgcct caggggttca
     1681 agatggacaa ggatgggctt ggcaccgtgt catccaaggg tgacaccctg tcggtgggtg
     1741 aatgggaagg gagcggcacc ccggcctccg tcctagagtt ctacaagaag acgatgccgg
     1801 gtttgggttg gaccgaagac ctctcgttca gcactgagac cggtggccag gtcagctaca
     1861 ccaaaggcca gagccaggcc gtggtgacca taaacaagtc ggacagcacc acccagctct
     1921 cgattatggt gggcaagtcc ggcagcctga agagcaattc gtcgtcggca ggcggttccg
     1981 gcgcgggggc ctcatccgga accaacattg cgaccacagg gtccactccc gcgccggcgg
     2041 ctgctcccac caaggctgcc gccgccacgc cgaagcctga ggcgaccgcg actcccgcgg
     2101 cccccaagtt gggatcagcg tcctctgtgc ccgatgaatt gaagcaggtc cctctgcctt
     2161 caggcttcag cgtcgtcgac gggagcatga tgcggcaggc cgcgggcggg gtatttgcat
     2221 tggccacagg gaagtggttc ggcaagagca gcgtcgcgga cgtggtcaag ttctacaaga
     2281 ccgcgatggc gaacgactgg gacgaggatt acgtcacgac atccggtgat gaggcgatct
     2341 gcacgtacac gaacaagaag aagcccgagc tttcggcgga gatcgaagtc atgaaagagg
     2401 aagcgggcac cattgtgacc gtttcactca cgcaagacaa ctaagtggtg catccggcaa
     2461 gttcatggca gttccaattg ttaccctggg cggagcgaag ggtctccggt cgcgaggaga
     2521 agcgtcgctc agcttgagag tgccgtgaac ttgccggaca agccactcag tcgctggtga
     2581 cactgaacgg aatcgaggca tcagccggaa cgtttctcgc ggctacggct gatgcctcga
     2641 tttcttttgg tgtcgctctc cgcccttccc atcgccagtc tgctggtgtc taccacagtt
     2701 ccgagaactg gtgagggttg aaggcgggga atctgcacaa atcaccggat tctccccttc
     2761 caggcctatt catcccggcc caatctcccg atttactatt gtagggacaa ctcgggaggg
     2821 gaagattgcg cgactcagcg gttctcgctt tgctgctgct tgctatgctc gtcgtggttg
     2881 gttgtgggga gtctgctcac gctaccggag tgaatgacgg cccgagcgtc ggccagacgg
     2941 acatcacaag gagcactgga gagccgtctg cagttggtcc ccagtatccc gacgcactca
     3001 agaagttccc cgtgccccag ggcttcgccc tcggcaaggg gggccatgcc acggtgatgc
     3061 agcgcggcga cctggtcgcg gttgccactt ggaagggaag cggcagcatc tcgggcgtcg
     3121 tcgagtacta caggcagagc ctgcccaagc agggctgggg agagaacgtc accttctcct
     3181 cggaccagag cgcacagttc acctacacga aggatctgtg ccaggcaacc accctcgtcg
     3241 aaaaggatgg caacgacatc gtgatcagcg ttgccattgg caggacctcc gaggatatcc
     3301 ccacgcctgt tcctactgcc actccagtgc ctactccaac ccgagtaccg acagccaccc
     3361 ccaagggtgc tgtaggaagg aacgtgccgc ctaccgttgt ccctctggcc acattcaccc
     3421 ctcagccgct cagccgtggg gatgcgtcga cagtgcccgc ggaactgaaa gacgttccgg
     3481 tccctggtgg attcacattg gtggacggca gcgcgcgccg agtcgctcag ggcggtgtgt
     3541 tgggcatggc ggcagcagac tggttcggca agagttcccc gaaggagatc ggcgcatact
     3601 atcgtagcgc gttggcgaag gggtggaacg acgacagttt tgtggagtct gcgaacgaag
     3661 tcaacgctca gtacacaagt aagaaggacg ctcgactgac tctcctgata gaagtgtcgc
     3721 tgttcgagtc tgacaccgta atccacatga gcctggtgcg aagccagtag ggaatgtcgg
     3781 tagcggatct gtcaccctga acggagtgaa ggatctctcc gaccacgagg agatccttcg
     3841 ctggtgctca ggatgacacc tgtccgttca ggatgaggat tacgccgtgg tcgtggagta
     3901 gttcgccgcc aagttggcca gcgccctcag cgcacctgcc atcccatccg tcagcctctg
     3961 caggtcctca gttgccaggt agccggacag ttgcctgact gtcgtggccg ttgcccccag
     4021 cgacgtcgtc agcaccacca gttcgtcccc ggaaggcttg ccttgcgata gctccaccgc
     4081 cctggattgc gtttgctcaa tatcgtgtgc cagcagatcg gctggggcag gacggttcag
     4141 ccctgtgagc gccctcgggg tcccgactac caccaggggt gtgccctgga cggccatggg
     4201 cgtgccgaag gccacggtgg gtccctgctc gttggtcgtc tgaatattgc tgctcagcac
     4261 cctcaccatt ccgtttatca actcgtccat ccgtttcatc gcctggtctc gctgcccggg
     4321 taccatttgc ggcaactgca ggctcacggt ctccatcagt tcggcggtgc gttgatacat
     4381 ccacactaca tcgtcccggc tgggcgaacc cgcggcagga ctttccatgc gacgctgcaa
     4441 ctcgaccgcc gctcgcagag tctgctcgac ctcgggagga ccctccaccg cggcaaaccg
     4501 ccccatgcta ggagcggcga cgacagtggg gattggcatg gcttgtcctg cggccgcggg
     4561 gatggtagcc gtcgctgcgg ccgcaggggc ggcggtgcta ctgggaacgg ggcgcagggt
     4621 ggcttctggt ggcggcgcgg gcgtctctgc gagcggaaaa ctgggcgtcg acgacgcgca
     4681 ccccgcgaca acggccatgg cgatcgcgac gagtccgtgc cggagacttc tgatccgttc
     4741 gctcaaccga cgtcacctca cggccgagtt tcaatattgg gcttttcatc ctcgaagtgg
     4801 ttgctgcaag aagcaagccc cacgctacct gcctccgcca cagggaggga tcgggtctgt
     4861 tgatgggcgt attgaggtgg cgctggtagc cttcgattgt atgtgcgctc tttcctgatc
     4921 gatccgggcg tgtaaagggg ggtgcgggat acggtaccgg agttcaacat cagcgttccc
     4981 atcaccggaa acatatggat agtcggcatt gtcttcctgc tgcacatcac tgccgtggcc
     5041 ttcatcatcg ggatagcgat gatagcgccg gtggcagagt tgctgggaat gaggacagga
     5101 ggcgagcgat gggagcggct ggcctatcaa ttgtccacga ttatcgagcg gatctttgcc
     5161 tggggcgcga cctgggcggc cttttccctg gtggctatct gggggctgtt cccccgcgtc
     5221 tggggctacc tatcctcgac cttcttcatc cagatcatca tcatcgcggg catcttgtgg
     5281 ttcgtgatga gtgtcactgc ctacctgtac tacgtcacct gggagcctct gcgacagaga
     5341 cgtctgctgc acaacgccat cggttggacc ttcgtcctgt tcaccatgat cttcatcacc
     5401 accatcgtga tgttcagttc ctatcaactc acccccaccg atgggggaga taggatgctc
     5461 actgccgccg cgaaccccag ctacggaacg gaagccgcgc atcgccacat aggcaacctt
     5521 tcctatgggg ggatcttgct ggccggattg ttgggagggt ggttactact gttcggagcc
     5581 gggggcgaag gggatcggcg ttccttccgc agttgggccg cggacgccgg cttctccgtt
     5641 ggtgccgttg tgctcctcct gatgcccttc agcggttggt tctacgcgaa tcagatccga
     5701 ctggcgtctc cgggcgcatt tgccctgatg atgctggggg acaacggctg gatgttccgt
     5761 gtccagatcg ggttgctggg caccgccttc cttctggcca accagtacat ctacctgaat
     5821 gttccgcgag acaggatcaa cgccgggcag ttgtcctgga tacgctactc cctgtgggtg
     5881 aacgtgctgc tgattggcct ggcggttctt cccaaggatg taccactcgg ccagatgaga
     5941 ccctggaagt acatagccct ggcagggttc atcctcttgc tgctcatcaa cctcacgaca
     6001 tacctgcgat ctcgccgggg agcggcctgg gtcacgagcg gcaggtggtc tcacgccgtc
     6061 ctggttgccc tggggatcgt cgttgttgcc ctgttcgtga tgatggggat catacggacg
     6121 agcgctcgcg gcaactggtt gatctacgag aggatgccgg tagatcaggg gcaggaactt
     6181 gtaaggccgt agggattgga acggtatgaa gacggaagct gccatcatct tcgtgatcga
     6241 gatcgctggg ttcgtcttct tcctggggct cttgccgttg gccgtcgtag ttggtcgact
     6301 tacccgggtg tggaggcgag atcgaggggt cggcctgctg ctgctggggg tactgatcaa
     6361 cacggccttc ctgttcttca cgctgttcgc tgcttttgcc ggtctggtcc tatatggggg
     6421 gtaatgcgtt gagtcagccg ggggtagcgg ttccgagttc cagccggcgg cgattcctgc
     6481 atcagttggg gattgcggtg gtggggggaa tcgtgagcct gctgaccgcg gttcccctgg
     6541 tcggctactt cttctccgtg ctcacgaaga agccttcgct ccaatgggtg aggctatgtt
     6601 ccctggacca ggtggactcg gagcagccga gggagttccg tatcaccttc cccggcgaga
     6661 atagttcggt gccctatcag gccgtgcagg gagtgttcgt tctgcgccat ggagatcaga
     6721 tcctggtctt ctccaacgtc tgcacccaca tggggtgctc cgttcgctgg cttgcgtggc
     6781 ggcagcagat catctgccca tgtcatgggg ggatgtacga ccggtggggc catctgatgg
     6841 gcgggcctcc ctcttccgac ctgcccctct acctttccag gatagaggac aacgacctct
     6901 acgtcgcgaa tcgcatcatt cagcgaggtg ttacgacgga agggggctga tgccatgttc
     6961 ggcggagtaa ttgacgtagt gtacggctgg ttggatagga gactcccttt cacgcgcaca
     7021 ctgaaccgag tattgaagga cccggtgccg gaacggggcg ggtggtggta caccctgggc
     7081 gccgcgatct tcgtgctact caccatccag ataatcaccg gtatcttctt gatgttctac
     7141 tacgtgccgt cctggaatga ggcgagggac tccattctct acatccagca gcaggtattc
     7201 ctgggctggc tggtcagggg tatccattac tggaaccagg tggctttggt ctttctggtg
     7261 ggggcacaca tggcacgcac cttcttctcc gcggcctaca aggcgccgag ggaactgatc
     7321 tgggtgctgg gtgtcacgct cctgctcctg atggttggca cggccttcac cggcggtatc
     7381 ctgcgctggg accaatcggg ttacttcgac gtcgtggtgg gaaccacgat cgcggggtgg
     7441 acccccatca tcggtcccgg cttggcgcag ctatggcgtg gtggagacac catcaccccg
     7501 cttaccctca cgcgcacctt cgtcttccac gtgtggctcc tgcctgcgcc actcttcctt
     7561 ctggcggtga ttcacgttgg cctggttgtc gtccagggcc agtatgggtc ctgggtcaac
     7621 tacgagcagg agccgcccga tgcccatccg atggacgagg acgagatcgc ctcccgcgag
     7681 aaggcgaagc gagagcttct cgatcctgag tcccacaagg tgaatcttcc cgtccgcacc
     7741 acctggttct tcccctatca cgtgttcaag gaagggattg tgtcgggtgt gatgttcctc
     7801 ggcgtctttc ttgccgcctg cttcttcgcg gtgccggtcg aggaggctgt ggatccatcc
     7861 accaccacct atgcccctag ctcgatgtgg ttctggctgt tcttggacca gatgtttctg
     7921 atcttccctg gggcctggat gatcccgttt ggagtcatcg ccgctcctgc catcatcgtg
     7981 accattttct atctgctgcc ctggctggat cgcaatccgg gtctgaagcc ctaccatcgc
     8041 cccgtggcgg tcaccttcat ggttgtgatc atcatgtcga ttctcgtgct tgcgatgctg
     8101 ggcgcgtccc gggtctacaa ctacgacttc gtgaacacgc cggggtagcg cccttcttgt
     8161 ggcgaatcga ccgatggtag gggcggacct gtgtgtccgc cctcgaccct gggcggacac
     8221 acaggtccgc ccctaccgat tcagaagaac cagggccggc cggcagcatg gaggcgcaat
     8281 cgaggtggaa cctaccatcg tacccgaggt ggcgcgccgc tttaccatag ggtttctgtt
     8341 tgttacgcac atccagttcg ccgcattcct gatcggcctg ttcagccttg ccgtctcctt
     8401 cgagttcttc tcgctgctca acagggggga tgagaatctg gatcgcttgg cccatggact
     8461 agcaaagacc gcggcctaca tgtacagcac gggcgcggtg ctggccttca tggtcatgtt
     8521 ctttgccact atcttctggc ccaccttctg gtacaccatc atccggatca acttctggcc
     8581 cttctttctg gaggccatca ccttcgctct gaccatcctg tacctctttc cctggtactt
     8641 cacgtggcat cgtctggcga acttcaggtg ggtccacctt tccctggggg cggctctgat
     8701 cgtttcggtc tacttccagc agagcctgat cgacgtggtg gctgcctaca tgcttacctc
     8761 cgtcccaccg gccctcttcc tgcgggtctt ctttaacccc accgtcatcc cgctggacat
     8821 gcacaggatg gtcggagaca tctccttcgc gggtttcgtc gttgcgggat acggggcatt
     8881 ccgaacgctt cgcgcccgcg aagcgaagga acgtggctac tacgattgga tgggcagtct
     8941 gggtttgatc gcaggactgg ccttcatgtt tttgcagccg gctatcggga tcgagtatct
     9001 gcaggagatt cggtcggcct cgccgggcgc gttcagcgtg atgatgcggg gacacaattc
     9061 ctggctattc ctgctccagg tgctcttcct gagtatcctg ttcgtggcga gtatggtgta
     9121 catactcctc caggtgagaa agagcggcac ttcaggcgtc cgctggctga ccgggctgct
     9181 gatcgtggcg atcctctccg cccttctgtt ggtgcagccg agggtgatcg ggcccagcca
     9241 ggagtacatg tgggtcaact gggtcaatcc catcggcgcg atgcaaccct ggaagtacat
     9301 cgccttcgcg ggaatgaccc tcagcggaat agccgctgtc gcgatctaca ccggcaagta
     9361 caggagaggg cttcgatggg gatatctgga aaggggaggt cggggtagcc agtacgtcct
     9421 gctgggtctg gccctctttg cgagcgggat gatggcgctc atggggtaca tcagggagaa
     9481 ctcccggata ccctacctga tctactacca gcagcgcctc gaccaaccgg agcgtttccc
     9541 tgagttgcgg cctacgccga ctccaggcct tggcggtttc ggcgtcgagg ggcagccgga
     9601 gggtggagcc caatgaagac ctgcctgtct ctcccgggta tccgcaagtg gtcgctgatt
     9661 gcgttcctgg cgttcctgtc accggtcgcg acctcctgtg gggtcgttta cgtcccgctg
     9721 cagccctatc ccctaatcgg ggagggcccc ccgcctggat acacgggcgg taccgttccg
     9781 gagcgctaca gcgccatgcg tgatccattc cttccgacgg accaaacggc attgcaggcc
     9841 ggcaaatccc tgtatgcctc acacgagccg agttgcgcga cctgccatgg cgatagaggg
     9901 catggcgacg ggccgctcgc ggcctacctc gagcctcagc ccgccgattt caccgcccct
     9961 cccatgttga acgcgttccg caatcaccag ccatacgtct actggtgggt aagcgaggg
//
LOCUS       DATALS010000411         6565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_112320, whole genome shotgun sequence.
ACCESSION   DATALS010000411 DATALS010000000
VERSION     DATALS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112320"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VHS06_09180"
     CDS             <1..208
                     /locus_tag="VHS06_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988329.1"
                     /translation="YLDEYTFRCNRRSSKKRGLLFYRLIEQALMVDHTPYERLLAPNH
                     KGRYVGDTRISQRRRSRSGVLALQ"
     gene            329..769
                     /locus_tag="VHS06_09185"
     CDS             329..769
                     /locus_tag="VHS06_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988330.1"
                     /translation="MEVAEHQPTHDRDEGRASSGNRPLDGQDGCQLFAWLWGRLSAVL
                     GTPSAAALMRRAISVAGCNYPILHGIAISRDRLTYIYNLPEELCTGGQEAPVPAYRAL
                     IKEVLSLTGELTCGIMVRGLVKSPELAPWLPDIQEVDKWLTSEE"
     gene            748..2208
                     /locus_tag="VHS06_09190"
     CDS             748..2208
                     /locus_tag="VHS06_09190"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX2988331.1"
                     /translation="MADLGRVRIQRLSTGDAELDGILGGGFPLQSVNIVAGGPGTGKS
                     ILTLQTLFYHARRGKKVLYFTTVSEPAIKLIRYMQQFDFFDADLVDERVFFYDLGSPA
                     RSEGLDRALDVCIEQIEERQPNLVAFDSFKALHDLARDPELSRAFVYDLAVNLATYQT
                     TSFLVGEYYEEDIRRYPEFFIADGIVYLALERENLRASRQIEVLKLRGASPVQGRHFF
                     DITASGIVVHPRVRAPEDQDEEDETEEVARLAVGIPGLDEMMDGGVPARSATLVEGGT
                     GTGKTLISLSFLMEGARNGEPGVLFTMEESRPQVLRMAAGRGWNLRELERQGLMRVVY
                     TSPIELAGDALLQEARRVIAELGAKRAVIDSLSSLKLSIGSEGRFKELVFALVKTFRS
                     EGLTLFLTNEVPELLGSVAITGHGVSSIADNVVLLRYVEVGADLKRALSVLKMRGSAH
                     QMQLRELLISATEIKVDGPFKDLQGVLTGVPVQTKD"
     gene            2233..3495
                     /locus_tag="VHS06_09195"
     CDS             2233..3495
                     /locus_tag="VHS06_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX2988332.1"
                     /translation="MPTTATADASRNVTQLVRVLRDMNNVLTTAEDWDERVGYVLGDL
                     NKLLPMDLAALLLPGGEDRLLVYLNSPLESSLVESVKLNVAEHYRLLTEQSVHPKALS
                     TEILGPHKPADPQAEPEDGNGELRNGSYLSLPMLGVDEIVGVIHVFSLKPNVYDEEKL
                     NLFSLVAAKLGNYLAHVNRNRVMQEYDRERDSFMGEVIHELKNPLTSATGFTQLLLRQ
                     RQKAGAVDREKETEMLERVYQQLLRVVQLADSVLDVTKIRAGWMALERERVDLGELAR
                     RVAETAQATAESNKIVVEVSGDCTVWGDEGRLEQVLQNLLSNAVKFSPAGGYIWVRTA
                     FDEVTEKVTVSVKDQGVGIPIEQQQRLFERYFRADTATKRRIGGSGLGLYISSEIVRR
                     HGGRIHVQSEIGKGSTFSFSLPAAKMES"
     gene            3658..4704
                     /locus_tag="VHS06_09200"
     CDS             3658..4704
                     /locus_tag="VHS06_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX2988333.1"
                     /translation="MLSGYFNRVAKESDTRFWINLPTAKEMELAIAAGAINCTTNPTY
                     GSRLIDEEPEYFAGLMDAAIAETDSNDVAAEKSYFWACGRIMRRFRPMFDASGGKEGF
                     VTVQGDPRRDHDPDWIVAEALRGSKLGPNFMAKIPATVAGIEAIARLTEMNVPICATE
                     VFAVSQAIQVCDRYQQAAERSGHHPPLYLTHITGIFDRYMSEFVQREKIDISPELLSQ
                     AGWAVAHEQYRAVKERGTGVIMLGGGALNNSHFTEMVGGDMHVTLNWSIVKSLLDADA
                     PVVSRITAPAPEDVVAELSEKLPDFRKAFYADALTPNEYEGYGPLQLFRTMFLNGWMR
                     MVNQIAARRKAGAA"
     gene            4903..5658
                     /locus_tag="VHS06_09205"
     CDS             4903..5658
                     /locus_tag="VHS06_09205"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2988334.1"
                     /translation="MATQLRTRRRGFGQAVTEEQPPGPVGQLPGRLERVLPALGGTAM
                     ALMGLTRRSPSGLAMAVAGSAIAYRAATGHAPLPNWVTSRLPRQGVQGLVVDRGFTVL
                     RPREELYQFWRNFENLPHFMTYLKSVTPSGDGRSHWVAAGPAASTVHWDAEIVEDRPN
                     ELIRWQSIAGSQVANRGEVRFDPAPGDRGTEVRVHLEYEPPAGVVGATIAQVLGQGAD
                     RQVRESLRQFKSLMEAHEIPTNDDQPWGTCLLS"
     gene            5683..>6565
                     /locus_tag="VHS06_09210"
     CDS             5683..>6565
                     /locus_tag="VHS06_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011321167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2988335.1"
                     /translation="MKAVCWCGKDSLKVENVPDPKIQKPRDAILRVTSTSICGSDLHL
                     VGGYIPTVQPGDIVGHEFMGEIVEVGSGVTNLQPGDRVVVPSPIACGECYFCKQQLWS
                     LCDNSNPNAWMSEAIMGYTTSAIYGYSHALGGYAGSFAEYVRIPFADVNHIKVPRDSL
                     ADDKLLFISDAFATGYMGADFCEIKPGHVVAVWGAGAVGLLAMKSAKLMGAERVIAID
                     RYPNRLNRARQHFGADETINYEQQPDVVETLEQMTAGRGPDACIDAVGMEADSPGPDN
                     LFDRAQQAMRTQMERPHV"
BASE COUNT         1342 a         2021 c         2101 g         1101 t
ORIGIN      
        1 ttatctcgac gagtacacct tccgctgcaa ccgccgcagt tccaagaaac gcggcctact
       61 cttctatcgg ctcatcgagc aggcactgat ggtcgatcac acgccctacg aacggttact
      121 agctcctaac cacaagggca ggtacgtcgg cgacacgagg atctcgcaac gtcgccgatc
      181 ccgcagcggg gttttggcgc tacagtagct ttatggatag cccctttgtt gaagtcggac
      241 atgtccgaat gaggactgtg ccgatgggcg cggcctagag tatgcgcata cagtatccac
      301 atgccggtcc actggagggc gatggctcat ggaagtcgct gaacaccagc caacgcacga
      361 tcgggacgag ggccgcgcga gctcaggcaa ccgccccctc gacggccagg atggttgcca
      421 gctcttcgca tggctatggg gaagactatc cgcggtgctg ggcactccct ctgcggcggc
      481 cttgatgaga agagctatct ccgtcgcggg ctgcaactat ccgatcctcc acgggatcgc
      541 catctccagg gaccgactca cctacatcta caacctcccc gaggagctct gcaccggcgg
      601 ccaggaagcc cctgttcccg cctaccgagc tctcataaaa gaggtattga gcctgaccgg
      661 agaactcacc tgcgggatca tggtgcgggg gttggtgaag agcccagagc ttgctccttg
      721 gcttccagat attcaggagg ttgacaaatg gctgacctcg gaagagtaag gatccagcgg
      781 ctttcgacgg gcgacgccga actggacggc atactgggcg gaggcttccc cttgcagtcg
      841 gtgaacatcg tcgcgggggg gccgggcacc ggaaagagca tccttaccct ccagaccctc
      901 ttctatcacg cccgtcgagg caagaaggtg ctctacttca ccaccgtctc ggagccggcc
      961 atcaagctga tccgctacat gcagcagttc gacttcttcg acgcggatct ggtagacgag
     1021 cgggtgttct tctacgacct gggcagcccg gctcgctccg aggggctcga cagggcattg
     1081 gacgtgtgca tcgagcagat cgaggagagg cagccgaacc tggtggcctt cgactccttc
     1141 aaggcgctcc acgatcttgc gcgcgacccg gaactctccc gcgccttcgt gtacgacctg
     1201 gcggtcaacc tggccaccta ccagaccacc tccttcctgg tcggcgaata ctacgaggag
     1261 gacattcggc gatatccgga gttcttcatc gccgacggca tcgtctacct ggcactggag
     1321 agggagaacc tgcgagccag ccgccagata gaggtgctga agctgcgtgg ggcttcccct
     1381 gtgcaaggcc gccacttctt cgacatcacg gcctcgggga tagtcgtcca ccccagggtg
     1441 agagcgcccg aggaccagga cgaggaggac gaaacagagg aggtagcccg actggcggta
     1501 gggatcccgg ggctggacga gatgatggac ggcggtgtcc ctgccagaag cgcgacgctg
     1561 gtcgagggcg gcaccggaac cggcaagacc ctcatctccc tctccttcct gatggaaggg
     1621 gcacggaatg gcgagcctgg ggtgctgttc accatggagg agtcgcgacc tcaagtcctc
     1681 cggatggcgg ctgggcgggg ctggaacctt cgtgagctgg agcgacaggg gctgatgagg
     1741 gtggtctaca cctcacccat agagttggcc ggcgatgccc ttctgcaaga ggccaggaga
     1801 gtgatcgcgg aactgggggc aaagagggcc gttatcgact ccctgtcgtc cctcaagctg
     1861 agcataggct cggagggacg cttcaaggag ctggtcttcg cgctggtcaa gacattccga
     1921 tcagaggggc tgacgctatt cctcaccaac gaggtacctg agcttcttgg gtccgtcgcc
     1981 atcaccggac acggcgtcag ctccattgcg gacaacgtcg tgctgctgcg atacgtagag
     2041 gtaggggcag acctgaagcg cgcgctctcg gtgctgaaga tgcgtggatc ggcccatcag
     2101 atgcaactca gggaactcct catctccgcc acagagatca aggtagacgg cccattcaaa
     2161 gatctccagg gggtgctgac gggggttccg gtacagacaa aggactaggt cgggcgacgt
     2221 taggaggcga acatgccaac tacagcgaca gccgatgcct cgagaaacgt cacacagctc
     2281 gtccgtgtgc tcagagacat gaacaacgtc ctgacgacgg cggaggactg ggatgagcgg
     2341 gtaggctacg tcctcggaga tctgaacaaa ctgctcccca tggatctggc agcgctcctg
     2401 ctgcccggcg gcgaagatcg tctcctggtg tatctgaact cgccgttgga gtccagcctg
     2461 gtcgagagtg tcaaactgaa cgttgccgaa cactaccgac tcctcaccga acagtcggtg
     2521 catcccaagg cgctctcgac tgagatcctc gggccgcaca agccggcaga tccccaggct
     2581 gagccggagg acggcaacgg ggagttgagg aacgggtcct acctgtcgct gcccatgctc
     2641 ggcgttgacg agatcgtggg agtgatacac gtcttcagcc tgaagccaaa tgtctacgat
     2701 gaagagaagt tgaatctatt ctccctggtc gccgccaagc tgggcaacta cctggcacac
     2761 gtgaatcgaa accgcgtgat gcaggagtat gaccgcgagc gagacagctt catgggcgag
     2821 gtaatacacg aactgaagaa tcccctcacc agcgccaccg ggttcacaca gctgctgctc
     2881 cgccaacgac agaaggctgg cgccgtcgac agggagaagg aaacggagat gctggagcgg
     2941 gtctaccagc aactccttcg agtcgtgcag ctggccgact ccgtcctgga cgtgaccaag
     3001 attcgagccg gctggatggc gctggagcga gagagggtcg atctgggaga gctggcgcga
     3061 cgagtggccg agactgctca ggcaacggcg gaaagcaaca agatcgtcgt ggaggtaagc
     3121 ggcgactgca ccgtctgggg ggatgaggga cgcctggagc aggtgttgca gaatctcctc
     3181 tccaacgccg tcaagttttc gccggccgga ggatacatat gggtgagaac agccttcgat
     3241 gaggtcacgg agaaggtgac ggtatcggtg aaggaccagg gcgtggggat cccgatagag
     3301 cagcagcaac ggctgttcga gcgctacttt cgggccgaca cggccaccaa gcgccggatc
     3361 ggcggctccg gactggggtt gtacatcagc agcgagatcg tccgccgcca cggtgggcgg
     3421 atccacgttc agagcgaaat cggcaaggga agcactttca gcttttcgct gccggcagcg
     3481 aagatggagt cgtgaccgca aggcacgtcg agtactggct cccgcattga tgttgatggt
     3541 agaatcgggg agcatcgttc gtcgacgcac atttggagac gaatagggca gtcgcccgat
     3601 ggtccgggaa agtccaaaag agagcccgcg ttttttcgac gcagaaagga caccgacatg
     3661 ctctccgggt acttcaatcg cgtcgccaag gaatcggata ccaggttctg gatcaacctc
     3721 cccacggcaa aagagatgga gctcgcgatc gcggcgggcg ccatcaactg caccaccaac
     3781 cccacctacg gctccaggct gatcgacgag gagcccgagt acttcgcggg gctcatggac
     3841 gcagccatcg ccgagacgga cagcaacgac gtggccgcgg agaagagcta cttctgggca
     3901 tgcggccgca tcatgaggcg attccggccc atgttcgacg cgagcggggg caaagagggc
     3961 ttcgtgaccg tccagggtga ccctcggagg gatcacgatc ccgattggat cgtcgcggag
     4021 gcgctgaggg ggagcaagct gggccccaac ttcatggcca agataccggc tacggtggca
     4081 ggtatcgagg ccatcgcacg cctgaccgag atgaatgtac cgatctgcgc caccgaggtg
     4141 ttcgccgtct cccaggcgat ccaagtgtgc gaccggtacc agcaggcggc agaacgaagc
     4201 gggcaccatc ctcccctcta cctgacccac atcacgggca tcttcgaccg ctacatgtcg
     4261 gagttcgtgc agcgggagaa gatcgacatc tcgcctgaac tgttgagcca ggcaggatgg
     4321 gcagtggccc acgagcagta tcgcgcggtg aaggagcggg ggaccggcgt gatcatgctg
     4381 ggcggcggcg ccctgaacaa ttcgcacttc accgagatgg tcggcggcga catgcacgtg
     4441 accctcaact ggagcatcgt gaagtcgctg ctcgacgcgg acgcgccggt agtctcgagg
     4501 ataacggctc cggccccaga agatgtggta gcggaactca gcgagaagct gcccgatttc
     4561 cgcaaggcat tctacgccga cgctctcacc cccaatgagt acgagggcta cggcccgctc
     4621 cagctattca gaacgatgtt cctgaacgga tggatgcgaa tggtcaacca gatcgcggcc
     4681 cgccgcaaag cgggagcggc ctgatccaca gagcaaccac atttccgcgg ggggggcggg
     4741 ctgcaatagc ccgcccctcg cctttttttc cctgcgattg cacggtaccg tcctctcgta
     4801 ccggcaacgt ctactttgcg gcacactcct tgccgggctt cccggcggtc gaccctcacg
     4861 tcccaatcgt cctccaacta cccggacatg gaaggatcga atatggccac acaactacgt
     4921 accaggagac gcgggtttgg gcaggccgtg accgaggagc agccgcccgg accagttgga
     4981 caactccccg gccggttgga gcgcgtgcta cccgctctcg gcgggaccgc catggccctc
     5041 atgggcttga cacgccgctc gccgagcggc cttgccatgg cggtggccgg cagcgccatc
     5101 gcgtacaggg cggcgacggg ccacgccccc ctgccgaact gggtgacttc acgcctgccc
     5161 cggcaggggg tgcagggcct ggtagtcgat cgaggcttca ctgtcctgcg cccacgagag
     5221 gagttgtacc agttctggcg caacttcgag aacctccccc acttcatgac ctacttgaag
     5281 tcggtgacac ccagcggcga cggtcgatcc cactgggttg ccgcgggccc ggcggccagc
     5341 accgtccact gggacgccga gatcgtggag gaccggccca acgaactgat caggtggcaa
     5401 tccatcgcag gttcccaggt tgccaaccgg ggcgaggtgc gcttcgatcc tgcacccggg
     5461 gaccgcggca ccgaggtacg ggttcacctg gagtacgagc cgccggccgg tgtcgtgggt
     5521 gcaaccatcg cccaggtcct ggggcagggc gcggatcggc aggtgcgcga gagcctgcgc
     5581 cagttcaagt ccctcatgga agctcacgag atcccaacca acgacgatca gccctggggc
     5641 acctgcctgc tgagctaacc cagcaaagga ggatagacaa ccatgaaggc agtgtgctgg
     5701 tgcggaaagg actccctgaa ggtcgagaac gtccccgacc caaagatcca gaagccgcgc
     5761 gatgccatcc tgagagtgac ctcgacctcc atctgcgggt cggatctgca cctcgtgggc
     5821 ggctacatcc cgacggtcca gccgggcgat atcgtcggcc acgagttcat gggagagatc
     5881 gtcgaggtcg gctcaggagt caccaacttg cagccgggcg acagggtggt tgtgccttcg
     5941 cccatcgcct gcggcgagtg ctacttctgc aagcagcagc tgtggtcgtt gtgcgacaac
     6001 tccaacccga acgcctggat gtccgaggcg atcatgggct acaccaccag cgccatctac
     6061 ggctattccc acgccctggg gggctatgcc ggcagcttcg ccgagtacgt ccgcatcccc
     6121 ttcgccgacg tcaaccacat caaggttccg agggatagcc tggccgacga caagctgctc
     6181 ttcatctccg acgccttcgc caccggctac atgggcgccg acttctgcga gatcaagccc
     6241 ggccacgtcg tcgccgtctg gggtgccggc gccgtggggc tcctagcgat gaagagcgcg
     6301 aaattgatgg gcgccgagcg ggtcatcgcc atcgaccgct atccgaaccg gctgaaccgt
     6361 gcacggcagc acttcggggc agacgagacc atcaactacg agcagcaacc cgacgtcgtg
     6421 gagacgctgg aacagatgac ggcgggacga ggtccggacg cctgcatcga cgcggtaggc
     6481 atggaggcgg acagcccggg gccagacaac cttttcgaca gggcgcagca ggcaatgcgc
     6541 acccagatgg aacgccccca cgtcg
//
LOCUS       DATALS010000412         2935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_337665, whole genome shotgun sequence.
ACCESSION   DATALS010000412 DATALS010000000
VERSION     DATALS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337665"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /gene="dxr"
                     /locus_tag="VHS06_09215"
     CDS             <1..1128
                     /gene="dxr"
                     /locus_tag="VHS06_09215"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX2988336.1"
                     /translation="IGRQTLEVIRALPDRLEVVGLAAGGFSDLFQEQLAEFHPMVSAV
                     GCPTPEQMAVLPQSVLTGVEGLVEVATIPDVDVVMIATVGRAGLAPTLAAVSQGKSVA
                     LANKESLVMAGSLVTDRARRSGARILPVDSEHSAIWQCLHGEGELGEWASTVKSLWLT
                     ASGGALRDMSPADVERVSPARVLAHPTWHMGPKVTVDSASLMNKGLEVIEAGWLFGLP
                     LDRIQIVLHRQSIVHSLVEFVDGALKAQLGTPDMRTPIQYSLTYPDRLPGLAAPLDLA
                     KAGALTFEDIDLTRYPCLRLALEAAKNGGSYPAALSSANEVAVQLFLAEKIWFGDIPV
                     LVEEVLSRHRPVQIDSLEAVLKVDEEARIECMNLARNRRRI"
     gene            1132..2220
                     /locus_tag="VHS06_09220"
     CDS             1132..2220
                     /locus_tag="VHS06_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2988337.1"
                     /translation="MNWTQLAVIPVLSFLLLVHELGHFIVARLSGIKVEEFGFGLPPR
                     IFGIRRGDMIYSLNWIPLGAFVRMTGEDGNNADQEGSFSSKPKRNRAAVLFAGPAMNF
                     LAAGFLFAAAYMIGWPTATQMDVFVYKVSANSPAADAGLQPGDRIVALDGQPLKTADE
                     FVTGTKSHLGTQVGVTVDRNGTQMEKQMVPRTQWPEGDGPLGIGLQGKVVETKPVSYG
                     VFSSVGMGFNRAWQMVTFTLSVPAMIIQGVLPADLARPIGPVGIYQITTQAADQAADN
                     GFWFPIISITATISAGLAVANLLPFPALDGGRLLLIGVAAVRWKRIAPEREGALHFVG
                     LMVLLALMVIISYYDVISPLPNIDWGLK"
     gene            complement(2304..>2935)
                     /locus_tag="VHS06_09225"
     CDS             complement(2304..>2935)
                     /locus_tag="VHS06_09225"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX2988338.1"
                     /translation="REGISDSRVLGAMEKVPRHLFVPPEGRRYAYRNVPLAIGDGQTI
                     SQPLVVALMTQALRLSGTERVLEVGTGSGYQCAILAELSRSVTSVERVAGLANASRVR
                     LARLGYDNIRIVVGDGSLGFPPAAPYDAILVTAAGPRVPDPLLHQLSPRNGRMVLPVG
                     EQDHQDLLLVTRCGDKISTSHLGNVRFVPLIGEAAWHERQVDLRLREDL"
BASE COUNT          529 a          815 c          986 g          605 t
ORIGIN      
        1 attgggagac agaccctaga ggtgataagg gcgctccctg accggctgga ggtggttggc
       61 ctggctgccg gtggtttcag tgatctgttt caggagcagt tggcggagtt ccatccgatg
      121 gtgtccgcag ttggttgtcc aactccggaa cagatggcgg ttttgccgca gagcgtgctc
      181 accggggttg aggggctggt ggaggttgcc actatccctg atgtcgatgt cgtgatgatt
      241 gccacagtgg gaagggcggg actggcgccc actctggctg ctgtctccca aggcaagagc
      301 gttgccctcg caaacaagga atcgctggtg atggctggat cgctggttac cgatcgggcc
      361 aggcgcagcg gggcccggat cctgcctgta gatagcgagc acagcgctat ctggcagtgt
      421 ctgcatggag agggcgagtt gggagagtgg gccagcacgg tgaaatcatt gtggctgacc
      481 gcctccggtg gtgctctcag ggatatgtct ccggcagatg ttgagcgagt gtcgcccgcc
      541 cgagtgttgg cccatcccac ctggcacatg ggacccaagg tcacggtgga ctctgcgtca
      601 ctgatgaaca aggggctgga ggtcatcgag gccgggtggc tctttgggtt gccgttggac
      661 cgtatacaga ttgtgttgca tcggcagagc atcgtccatt ccctggtgga gttcgtcgat
      721 ggcgcactga aggcgcaact gggcacgccg gatatgcgga ctcccattca gtactcgctg
      781 acctatcctg accgacttcc cgggctggca gcgcccctgg atttggcgaa agcaggcgcg
      841 ctgacctttg aggatattga cctgacgagg tatccttgtt tgagacttgc gctggaggct
      901 gccaagaatg gtggcagcta tccggcggcg ctcagttccg caaacgaggt tgcagtgcaa
      961 ctgttcttgg ctgagaagat ctggtttggg gacattccag ttctcgtgga agaggtgctt
     1021 tcgaggcatc ggcctgtaca gatcgacagc ctcgaggcgg tactcaaggt ggacgaagag
     1081 gcgaggatcg agtgcatgaa cctcgctagg aataggagaa ggatctagtc catgaattgg
     1141 acccaactgg ccgtaatacc ggttctgagc ttcctgctgc tggtgcatga gcttggccat
     1201 ttcatagttg cgcggctgtc ggggatcaag gtggaggagt ttggctttgg ccttccacct
     1261 cgaatcttcg gcatcagacg tggcgacatg atctactcgc tgaactggat cccgctcggc
     1321 gccttcgtgc gaatgacggg cgaagacggc aacaacgccg atcaggaggg gagtttctcc
     1381 agcaagccga agcggaatcg ggctgcagtc ttgttcgcgg ggccagccat gaacttcctc
     1441 gcggccggct tcctcttcgc cgcggcatac atgataggct ggccgaccgc cacgcaaatg
     1501 gatgtgttcg tctacaaggt cagcgccaat tcccctgccg ccgatgcggg attgcagccc
     1561 ggcgatagga tcgttgcgct ggatggacag ccgctgaaga ctgccgacga gttcgtcacg
     1621 gggaccaagt cgcacctggg gacgcaggta ggggtcacag tcgaccggaa cgggacccag
     1681 atggagaagc agatggttcc ccgcacgcag tggcctgaag gcgatggacc tctggggatc
     1741 ggactgcagg ggaaagtagt cgagacgaag ccggtttcct atggggtgtt cagctcggtc
     1801 ggcatgggct tcaatagggc gtggcagatg gtgaccttca ccttgtcggt tccggctatg
     1861 atcattcagg gggtgttgcc ggccgacctg gcgagaccta tcggcccggt tggcatctat
     1921 cagattacga ctcaggctgc cgaccaggcg gcggataacg gcttctggtt ccccatcatc
     1981 agcatcacgg caactatcag cgcagggttg gctgtcgcga atctgctgcc gttccctgct
     2041 ctggacggcg gccgactgct tttgatcggc gtggcggcgg ttcggtggaa gcggattgcg
     2101 cccgagcgcg agggtgccct gcatttcgta gggctgatgg tgctgctggc gctgatggtg
     2161 atcatctcct actatgacgt gatctccccg ctgccgaaca tcgactgggg tttgaagtag
     2221 cccgtcgtcg ccatgtggca catcgtcaca ctgataacgt agggcgggtc aggggcgacc
     2281 cgccctacag gtttgtgtgt cctctacagg tcttctcgga ggcgcagatc cacctgccgc
     2341 tcgtgccagg ccgcttcacc gatcagaggg acgaaccgaa cgttgccgag atggctggtg
     2401 gatatcttat ccccacatcg cgtaacgaga agcagatcct ggtggtcctg ttcgccgacg
     2461 ggcaacacca tgcgtccatt gcgtggcgag agctggtgca gcaagggatc cggtactcgt
     2521 ggaccggcgg ccgtgacgag gatggcatcg tagggcgctg ctggtgggaa gcccagactg
     2581 ccatctccga ccacgatgcg gatgttgtcg tagccgagcc tggcaagccg gacgcgcgag
     2641 gcatttgcca gccccgccac tcgctccacg gaggtgacgc ttcggctaag ctcggcgagg
     2701 atggcgcatt gatagccgga acccgtcccc acctcaagca ccctctcggt gccgctaagc
     2761 cggagcgcct gcgtcatcag cgcgaccacc agcggttggg agatggtctg cccgtccccg
     2821 atggccagcg gtacgtttcg gtaggcgtac cggcgaccct cgggagggac gaagaggtgc
     2881 cgagggactt tctccatggc tccgagcaca cggctgtccg atatcccctc tcgcc
//
LOCUS       DATALS010000413         8993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_403783, whole genome shotgun sequence.
ACCESSION   DATALS010000413 DATALS010000000
VERSION     DATALS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403783"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2411
                     /locus_tag="VHS06_09230"
     CDS             <1..2411
                     /locus_tag="VHS06_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX2988339.1"
                     /translation="SGQRAIAALRKAVPIRARVQRMGELVSVDASELVPGDTVQLLPG
                     DPVPADGRLLDTHRLQLEESTLTGESGPVEKTVAPVEPDAPLADQRSMVYRGTTVVAG
                     RGKALVVATGRDTAVGRLHALAAEAAAPPTPLTRDLDNLSRVLAISAAGISVGVVGLS
                     MLRGVPLVRALSTAVALGVAALPEGLPTTATTVLALGSGRMRRKGTLIRSLNAAETLG
                     SVTLVCADKTGTLTENRMSVGEVMVDGTAIQVSGPADSPEGRLYSSRGAVGIENSPAL
                     SELLKVAVLCSDAEVAGTQNGKVSIDGSATEGALLVLAMKAGLDVDRLRADFPRIDRR
                     DRGDGRRYMVTVHPGIEGPIALAKGAPDEIVEICRSAFSDGSIVPLDASIRDKWLRHN
                     AMLAARAMRVLAFASRQLPPHYTDADLASGFTWCGLVGLVDPIRPGVLEAIAALRKAG
                     INTIMVTGDQATTAAAIARQLGLDQPSPLHVLEAGDLATMDEEMLRGLVHHVDVFARV
                     PPEMKLKVVRALQASGEIVAMTGDGINDAPALRAADVGIAMGERGTELARELADVVLS
                     TDDFPKVVDAVEEGRLVRVNVRRVLHYLLSTNASEVWAVIAAVSAGLPAPLTPGQLLW
                     LNLITDLAPAIGLAMEPRDPELMTQPPRPPAEPIVPAPLRRRILGESAAIAAGSLAAY
                     VAAIMRHGPGPVAQTSAFTSLATTQLLQVGLARAGAHPALGYKRAWNRPIALGVGSSA
                     LLQAAALLVRPLRTLIGGAPLGLLDWIVSLSTGLIPVLGIELQRQGAARVSPPAARSS
                     ERLS"
     gene            2421..3602
                     /gene="metK"
                     /locus_tag="VHS06_09235"
     CDS             2421..3602
                     /gene="metK"
                     /locus_tag="VHS06_09235"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX2988340.1"
                     /translation="MSQSRFFTSNSVMLGHPDKLCDRISDALVDAHLSMDPEARIRAE
                     VAAAGQLIFLVTDVDIHGEVDVTGVVRRAIAETGYFPADMDPDRCAILSQSSRMTPLG
                     ASVSAGPEVFTEEGVATEQTTVFGYACSETPERMPLPIQVAHRLSVTLSVLAQQRTIP
                     GLGPDGDVQVTVEYDGNRPIRIDTMVIQLQHRGNPSDLRESIQRLALDPVTQSLPVKP
                     DSSTRLILNPAGPLEVAGPARDAGHTGRKNADDSYGGAARHGGHSLSGKDPGRLDRSA
                     SYAARHVATNVVAAGLATECELMLSYAIGQTTPSTVFARTLGTGVISDERLSEIVGSV
                     FDLRPGAIVKRLGLRQLPREKGGRFYQLLAVGGHFGRIDLDPPWELTDHVDALREAAR
                     V"
     gene            3654..4154
                     /locus_tag="VHS06_09240"
     CDS             3654..4154
                     /locus_tag="VHS06_09240"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX2988341.1"
                     /translation="MRLDSPEPFIAPTSPALLTTGAYFMPEMPAGPGSSPPRRVEPDS
                     FNRRVYSIVRRIPRGMVTTYGIIAQALGDPRKAREVGWALNAKPKGESAPAHRVVNRE
                     GKLSGGWAFGSPAVQRGLLEEEGVSFLQDGRVDLEGQLWRPGECEGIQPLKEPSLFDL
                     FEEERE"
     gene            4151..4864
                     /locus_tag="VHS06_09245"
     CDS             4151..4864
                     /locus_tag="VHS06_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HEX2988342.1"
                     /translation="MTPERGNQTIPHGRPATGMLHLVRRVVLWVFLVGCGAALAVVLP
                     ALWVFITGSSAIYDTPTQVPSMPVAIVFGAGLQVDGSPSWMLADRVDAAAGLYEAGKV
                     QRILMTGDNSSIGYNEVAAMKQRAIEKGVPADKVNLDYAGFRTYDSCYRARAVFGITK
                     AVLVTQRYHLPRALFLARAFGVDAVGLVAGRDYYPRQEYYDAREAAAMSVSWYEVNVT
                     HPLPRYLGDPVDLERQNNR"
     gene            complement(4903..5673)
                     /locus_tag="VHS06_09250"
     CDS             complement(4903..5673)
                     /locus_tag="VHS06_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988343.1"
                     /translation="MTERRPILRMENVSYAYMGRYPALGGVDLTVARGERLVILGANG
                     CGKSTLLKIADGLIRPDRGRVEACGEDAVAAVGDAAVARRLHRRVGLVFQDADVQLFS
                     PTVWDDVAFGPLQMGWDEAEVQRRVEAALQLMGIEALARRAPYELSEGEKKRASIATV
                     LSLDPEMLLLDEPTANLDPRSKAVLLDLIVRLSEEGKTVVTTTQELEIAPEVGRRAVV
                     FGYNERHQVADGPVLEILEDTDLLVDANLIHPRLLWKR"
     gene            complement(5679..6548)
                     /gene="cbiQ"
                     /locus_tag="VHS06_09255"
     CDS             complement(5679..6548)
                     /gene="cbiQ"
                     /locus_tag="VHS06_09255"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEX2988344.1"
                     /translation="MVGDLEPRKGGRREGDFAARTVAGVAATLERALFSEEQARLPGL
                     LQGVDPRAKVGAALALLLAVGLARHVAVVAAICALALFLAALSRLELLPYARRVWLGI
                     PLFAGVVVLPSLFMLPGTPLLTLLDTPSVRLAVSDNGVASAMTLILRVGASVSVALLL
                     VFTTPWTELLRAFRVLRLPESFVVVLGMTYRYLFLFLHAANSLFLARASRTVGATSGG
                     EQRRWAAGATGSLMGRSLKMSGDVLLAMRARGFDGQIRTSGLPAMQDGDWLLLASGVM
                     LSACLLLIDMGVR"
     gene            complement(6550..7590)
                     /gene="cbiM"
                     /locus_tag="VHS06_09260"
     CDS             complement(6550..7590)
                     /gene="cbiM"
                     /locus_tag="VHS06_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HEX2988345.1"
                     /translation="MHIPDGYLSPATVVVAYGAAAPFWYQAGKKVKETLQGQSAPLVA
                     LFAAFSFTIQMFNIPVPGGTTAHAVGGTLMAAVLGPWAAVVGLSTALVIQALFFGDGG
                     ITAIGANCFNMGIALPISGYFLYRLLSGSSPSPRRRAIAAAAGGYLGINVAGLLTGIE
                     LGIQPLLWSDGGRALYNPYGLDVTVPAMLLVHLTLAGFAEAALTGLGVAFLLRSHPWL
                     FSTESTGTRMLGLGKTGWSLVSALGLLVVLTPLGLLAPGGADFEWGAEELTKMVGYLP
                     AGFSRLADSWQFAPFRDYQMAGVAGDSSFAQQASAYILSAVAGVSLIFVLLFAARILL
                     ARSSSAARGERT"
     gene            complement(7841..8311)
                     /locus_tag="VHS06_09265"
     CDS             complement(7841..8311)
                     /locus_tag="VHS06_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX2988346.1"
                     /translation="MCTSTSVGVGKKLSPEDRRHIVVALQDNGLRLTTQREAVCEAIF
                     GCSGHICAEHILESVTAHHPELRMNKTTVYRALDLLLELGLILEHKCGDGRAQYEPAS
                     RGRHSHMICRQCGRLLDMDGAVASAIREQLVDAHGFRVELESYPIFGICPDCRG"
     gene            complement(8582..8902)
                     /locus_tag="VHS06_09270"
     CDS             complement(8582..8902)
                     /locus_tag="VHS06_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2988347.1"
                     /translation="MTWEERKNPKNGMSNFIKILHQDDETGMRVNLCFYPKGFVTKWH
                     THPCAHGMYVLEGILKTHEGVYGPGSFVWFPEGIVAEHGATAETDVLTLFITNKRFDI
                     TYKD"
BASE COUNT         1737 a         2986 c         2787 g         1483 t
ORIGIN      
        1 gatcggggca gcgagccatc gcggcgctgc gcaaagcggt gcccataagg gcgcgcgtgc
       61 agcgcatggg agaacttgtt agtgtcgatg cctccgaact ggttccgggt gacacggtac
      121 agctactccc cggcgacccg gtccccgccg acggacggtt gcttgacaca caccgactgc
      181 agctagagga gtcgacgttg accggcgaat ccggcccggt ggagaagacc gtggctcccg
      241 tggaacccga cgcgccgctg gcagatcagc ggtccatggt gtaccgagga accacggtag
      301 tcgcgggcag gggcaaggcg ctcgtggtgg ccacagggcg ggacaccgcg gtaggccggc
      361 tgcatgcgct ggccgcggaa gccgcagcac cgcctacgcc gctgacgcgc gacctggaca
      421 acctgagcag ggtactggca atctcggcag cgggtatctc tgttggagtc gttggtctct
      481 ccatgctgag gggcgtgcct ctcgtgaggg cgttgtccac ggcggtggca ctgggagtgg
      541 ccgcattgcc ggaaggactc cccaccaccg cgacgaccgt tctggcgctg gggtcgggtc
      601 gcatgcgaag gaaaggcacc ctcatccgat ccctcaatgc tgccgagacg ttgggatcgg
      661 tgacactggt gtgcgcggac aagaccggca ccctcaccga gaaccggatg agcgtaggag
      721 aggtcatggt agatgggacc gcaatacagg tgagtgggcc ggccgactca ccggaaggaa
      781 ggctgtacag cagccgcggc gcggtcggaa tcgagaatag cccggcgctt tccgaactac
      841 tgaaggtcgc cgtcctctgc tccgacgcgg aggtggccgg gacgcagaac ggcaaggtat
      901 ccatcgacgg gtcggccacg gagggagcgc tgctggtcct ggccatgaag gcggggctag
      961 acgtcgacag actgcgggcg gacttccctc gcatcgacag gagggatcga ggtgacggcc
     1021 ggagatacat ggtcaccgtc catcccggca ttgaggggcc cattgcgctg gcaaaagggg
     1081 ctcccgacga gatagtagag atctgccgga gcgcattcag tgatgggagt attgtgcccc
     1141 tggatgcctc catcagggac aagtggctga ggcacaacgc tatgctggcc gcgcgagcca
     1201 tgcgcgttct tgccttcgcc tcgcggcaac tgcccccgca ctacaccgac gcggatctcg
     1261 cctcaggctt cacctggtgc ggcctggttg ggcttgtgga ccccatccgg ccgggggttc
     1321 tcgaggcaat cgccgccctc cgaaaggcag ggatcaacac catcatggtg acaggagatc
     1381 aggcaacaac cgcggccgct atagctcgac agcttggact ggaccagccc agccctctgc
     1441 acgtcctgga ggccggcgac ctcgcgacga tggacgaaga aatgttgcga gggttggttc
     1501 accacgtgga cgtgttcgcc agggtcccgc cggaaatgaa gctcaaagtg gttcgggcgt
     1561 tgcaggcaag cggcgagata gtcgccatga ccggagacgg tataaacgat gccccggcac
     1621 tgcgggccgc ggacgttggg atagcgatgg gagagcgtgg caccgagttg gccagggagc
     1681 tggccgacgt agtgctatcc accgacgact ttccgaaggt ggtagatgcc gtcgaggagg
     1741 gtcggctggt cagggtcaac gtccgcagag tgttgcacta cctgctctcc accaacgcca
     1801 gcgaggtgtg ggctgtcata gctgccgtgt ctgccggcct gcctgccccc cttacaccgg
     1861 gccagctact gtggctcaac ctgatcaccg acctggctcc cgccataggc ctggccatgg
     1921 agccccgcga ccccgaattg atgactcagc cgccccgccc tccagcggag ccgatagtcc
     1981 cagcaccact ccggcgccgc attctgggcg aatccgcggc catcgcggcc ggctctctgg
     2041 ccgcatacgt ggcagccatc atgcgtcacg ggccggggcc ggtggcacag acgagcgcct
     2101 tcaccagcct ggccaccacg cagctgttgc aggtcgggct cgcacgggca ggtgcccatc
     2161 ccgccctcgg gtacaaacga gcatggaacc ggccaatcgc gctgggagtc ggcagttcgg
     2221 ccctgctgca ggcggcagcg ctgctggtac gcccacttcg cacccttatc gggggcgcgc
     2281 cgctggggct gctggattgg atagtgtcct tgagcacggg cctcataccc gtcctgggga
     2341 tcgaactcca gcgtcagggt gccgctcgcg tgtctccacc ggcggccaga tcgtctgaac
     2401 gacttagcta gggggtagcc atgtctcaga gtcgcttctt cacatcgaac tcagtgatgc
     2461 tgggccaccc tgacaagctg tgcgaccgca tctccgatgc tctcgtagat gcgcacctct
     2521 ccatggaccc agaggccagg atcagggcag aagtggcggc cgccggccaa ctcatcttcc
     2581 tggtaacgga cgtcgacatt catggagagg tggacgtcac cggcgtcgtc cgccgagcca
     2641 tcgccgagac aggctacttc ccggcggata tggatccgga ccgatgcgcg atcctgtccc
     2701 aatcaagccg gatgaccccg ctgggggcat cagtctctgc tggaccggag gtcttcactg
     2761 aagaaggggt ggctactgaa cagacgacgg tcttcggata cgcctgttcc gaaaccccgg
     2821 agcggatgcc actgccgatc caggttgccc acagactctc cgtcacactc agcgtcctcg
     2881 cacaacagcg caccattccc ggcctgggcc cggatggcga cgtgcaagta acggtcgagt
     2941 atgatgggaa ccgccccatc cgaatcgaca cgatggtgat tcagcttcag cacaggggca
     3001 atcccagcga ccttcgcgaa agcattcaga ggctggcgct ggatcctgtc acccagtcgc
     3061 tcccggtgaa gccggacagc agcacgaggc tcatactcaa ccccgcaggg ccgctggagg
     3121 tagccgggcc tgcccgcgac gccggccaca ccggccgcaa gaatgccgac gacagctatg
     3181 gcggcgctgc acgccacgga gggcactccc tgagcggcaa agacccggga cggctggacc
     3241 gttctgcaag ctacgccgcc cgccatgtcg cgacaaacgt cgtcgccgcg ggcctggcca
     3301 cggagtgcga gttgatgctc tcctacgcca tcggacagac gactccatcc accgtgttcg
     3361 cccgcaccct cggcacgggc gtcatctcag acgagaggtt gtccgagatc gtgggaagcg
     3421 tcttcgacct gcggccgggt gccatcgtga aacggctggg gctcaggcaa ctgcccaggg
     3481 agaagggcgg gcgcttctac cagctactcg ccgtcggggg acacttcggg cgcatcgatc
     3541 tcgatcctcc gtgggaactc accgaccacg tggacgccct cagggaagca gcccgcgtgt
     3601 gaggttgcac cacgcgcggt ccgtctgttg tatactcaac ctaacatcag cgcatgcgac
     3661 tcgacagccc tgagccgttt atcgcgccga ccagccctgc gttgctcacc acgggagcct
     3721 acttcatgcc agaaatgccg gccggaccgg gatcctcgcc tcctcgccga gtcgagccag
     3781 actccttcaa tcgccgcgtg tacagcatag tccggcgcat cccccgtggc atggtgacta
     3841 cctatggcat catagcccag gcgctgggcg acccccgaaa ggcccgcgag gtaggctggg
     3901 ccctcaacgc caagccaaag ggagaaagcg cccccgctca cagggtcgta aaccgggaag
     3961 ggaagctgag cggcgggtgg gctttcggta gccctgcagt gcagcgcggg ctgttggagg
     4021 aggaaggcgt ctccttcctc caggatggaa gggtagacct ggaaggacag ctctggcggc
     4081 ccggggaatg cgaaggcatc caaccgctca aagagccgtc actcttcgac cttttcgagg
     4141 aggagagaga atgacccctg aaagggggaa ccagacaatc ccgcatggca gaccggccac
     4201 cggaatgctg cacctagtgc ggagggtcgt gctatgggtc ttccttgtag ggtgtggagc
     4261 tgcgctcgcc gtggtactgc ccgccctctg ggtcttcatc accggatcta gcgccatcta
     4321 cgacactccg acgcaggtgc cgagcatgcc ggtggcgatc gtctttgggg cgggattaca
     4381 ggtcgacggc tccccaagct ggatgctggc ggacagggtg gacgcggcgg caggtctgta
     4441 cgaggcaggc aaggtgcagc ggatcctgat gacaggggac aacagctcca tcggctacaa
     4501 cgaggtagca gccatgaagc agcgagccat agaaaagggt gtgccggcgg ataaggtgaa
     4561 cctggactac gccggcttcc gaacctacga tagctgctat cgagccagag ccgtcttcgg
     4621 catcacgaag gcggtgctgg tcacacagcg atatcacctc cctcgcgccc tcttcctggc
     4681 tcgggctttc ggcgtggatg ccgtcggtct ggtggcgggc cgcgactact accccaggca
     4741 ggagtactac gatgccagag aggcggccgc catgtccgtc tcctggtacg aagtgaacgt
     4801 cacccacccc ctaccccgct acctgggtga cccagtggac ctcgaacgcc agaacaaccg
     4861 gtagcccgct cccccacgtc cctgtggttc cgcgtcgcga cgctaccgct tccagagcag
     4921 cctgggatgt atcaggtttg cgtccaccag cagatccgta tcttccagga tctctaggac
     4981 ggggccatcc gccacctggt gccgctcgtt gtaaccgaag acgaccgcgc ggcgaccgac
     5041 ctcaggcgct atctccaact cctgcgtggt cgtgacgacc gtcttcccct cctcggagag
     5101 ccgaacgatt agatccagca acaccgcctt gctccgcgga tcgaggttcg ccgtcggctc
     5161 gtccagcagc agcatctcgg ggtccaggga gaggacggtc gcgatagagg ctcgcttctt
     5221 ctccccctcc gaaagctcgt agggtgccct tcttgccaga gcctcgatcc ccatcaactg
     5281 cagcgccgct tccacccgac gctgcacctc agcctcatcc catcccatct ggagcggacc
     5341 gaaagcgacg tcgtcccaca ccgtaggtga gaacagctgg acatcggcat cttgaaagac
     5401 cagacccacc cggcgatgaa gtctcctggc gacggcggca tcacccactg cggccacggc
     5461 atcctcgcca cacgcctcca cccgcccgcg gtccggccgg atcaatccgt ccgctatctt
     5521 cagcagcgtg gatttgccgc agccgttggc tccgagtatc acgagccgct ccccacgagc
     5581 cacggtcagg tccaccccgc ccaaagcggg gtacctgccc atgtaggcgt aggagacgtt
     5641 ctccatccgc agtatcggcc gccgttccgt cacagcatct acctcactcc catgtcgatc
     5701 agcagcaggc aggccgaaag catcacgccg gacgccagca acaaccagtc gccatcctgc
     5761 atggcaggaa gcccgctggt gcggatctgg ccgtcgaaac ccctggctcg catggccagc
     5821 agcacgtcgc cgctcatctt cagcgagcgt cccatgaggg aaccggtagc tcccgccgcc
     5881 caacgtcgct gctcccctcc tgaggttgcg cccacggtcc ggctggccct cgcaaggaag
     5941 aggctgttgg cggcgtgcag gaacagaaac aggtatcgat aggtcatgcc gagcaccacg
     6001 acgaagctct ccggcaaccg cagcacccga aaggctcgca gcagctcggt ccaaggggtg
     6061 gtgaacacca gcaacagcgc gaccgatacg gaggcgccca cccgaaggat cagagtcata
     6121 gcactggcta ccccattgtc ggataccgcc agccgcacag atggggtatc cagcaacgta
     6181 agcagcggtg tgccgggcag catgaacagc gaaggcaaca ccacgactcc ggcgaagaga
     6241 ggaattccca gccacacccg cctggcatag ggtagcagtt cgaggcgcga cagcgcggcc
     6301 aggaacagcg ccagcgcgca gatggccgcg accacggcga cgtgccgtgc caaacccacc
     6361 gccaacaaga gtgccagcgc ggcgccgacc ttcgccctgg ggtccacgcc ttgtagtagg
     6421 ccgggcagtc gggcctgctc ttcgctgaag agtgcccgct ccagcgtcgc agcgacgccg
     6481 gctactgtcc gagcggcgaa atcgccctca cggcgaccgc cctttcgagg ttccaagtcg
     6541 ccaaccattc taggtccgct ctccacgggc agcggaactg gagcgagcca ggagtatcct
     6601 ggccgcaaag agcaggacaa agatcagcga aacccccgcc acggcggaca ggatgtaggc
     6661 agacgcctgc tgagcgaagg acgagtcgcc cgccactccg gccatctggt agtcgcggaa
     6721 gggtgcaaac tgccaggaat cggccagccg cgagaagccg gcgggcaagt acccgaccat
     6781 cttcgtcaac tcctcggccc cccactcgaa atctgcccca cccggagcca gcagcccaag
     6841 cggggtgagc actaccagca ggccaagcgc cgacacgagc gaccaccctg tcttcccaag
     6901 gccaagcatc cgcgtcccgg tcgactccgt ggagaacaac caggggtggg agcgaagcag
     6961 gaaggcgact ccgagtcccg tcaaggcggc ttcagcaaag ccggccagcg tcaggtgaac
     7021 cagcagcatc gcgggcaccg ttacgtcgag tccgtaaggg ttgtacaggg cgcggccccc
     7081 atcgctccac aacagcggct ggatccccag ctcgatcccc gtcagcagcc ccgcgacgtt
     7141 gatccccagg tagccgccgg ccgcggccgc tatcgccctg cgcctcggag atggactgct
     7201 tcccgacagc aatcgataca ggaaatagcc cgatatcgga agcgcgatcc ccatgttgaa
     7261 gcagttggcg ccgatggcag tgatgccacc atccccaaag aagagtgcct gaatcaccag
     7321 agcggtggac agtccgacta ccgcagccca cggccccagc accgcggcca tcagcgttcc
     7381 gccgacggca tgggcggtag tgccgcccgg gaccgggatg ttgaacatct ggatggtgaa
     7441 actgaaggcc gcgaacagag cgacgagagg tgcggactgc ccctgcagcg tttccttcac
     7501 cttcttcccc gcctggtacc agaagggagc agccgcccca taggcgacga ccaccgtcgc
     7561 cgggctgagg tagccatccg ggatatgcat ctcaacctcc ctttcgcagc cgtgggcacg
     7621 tcttctggag tgcggcgact tgacgcctca atgttgccct gctcaacttg ccggtgcact
     7681 gcagcgttgc cggttttggg agcgcaggcg tctcgcctgc atcaccgccc tgcccgcgct
     7741 ttgggggtcg gcttacgcct ccccccacaa agcggcgctc acgcgccgca ctccacatgg
     7801 actgcaacct agttgcaatt cgaacgcaac aaagactgag tcaacccctg caatccgggc
     7861 agatgccgaa aatcgggtag ctctccagct caacacgaaa tccgtgagcg tccaccagtt
     7921 gctcgcgaat cgccgatgct accgccccgt ccatgtccaa caaccgccca cactggcggc
     7981 agatcatgtg gctgtggcgt ccacgcgagg caggctcgta ctgggctcgc ccatccccgc
     8041 acttgtgctc caggatgaga cctaactcca gcaacaggtc cagcgccctg tacacggttg
     8101 tcttgttcat tcgaagctca ggatggtggg ccgtgaccga ctccagtatg tgctccgcac
     8161 agatatggcc ggaacagccg aagatcgcct cgcagacggc ctcgcgctgc gtcgtcagcc
     8221 gtagcccatt gtcctggagc gcgacgacga tgtgccttct gtcctccggc gacagcttct
     8281 tccccacacc gacactggtg gaagtacaca tggagcgctc cttcccatcc gacaattgca
     8341 actaggttgc agaattctac cgtttcccgc catatcgcgc aaccactgcg gagatgccgc
     8401 cagtattggg ccttcgaaca gtaggggcgg acccacgcgg cgcgttgtgt gtcatctgcg
     8461 cgctcgtgcg gctgacaaga gggatcccga atggtagggg cggacctgtg tgtccgccca
     8521 ggaccgaggg cggacacaca ggtccgcccc taccatcaga agattcacgg catgatcgag
     8581 gtcagtcttt gtaggtgatg tcgaagcgct tgttcgttat gaacagcgtg agaacgtctg
     8641 tttcggcagt tgccccgtgc tccgcgacta tgccttcggg aaaccataca aatgaccccg
     8701 ggccataaac gccctcgtgt gtcttcaaga ttccctctag cacatacatt ccgtgcgcac
     8761 atgggtgagt gtgccactta gtgacaaacc ccttgggata gaagcacaga ttcaccctca
     8821 tgccggtctc gtcgtcttga tggagaatct ttatgaaatt gctcattccg ttcttggggt
     8881 tcttgcgctc ttcccaggtc acagcatttg cgtctgttga gcggtatccc cgcgccatac
     8941 atcagcatcg ggatgatgat gatgccccct ccaagtccca ggaggcccgc cat
//
LOCUS       DATALS010000414         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_66417,
            whole genome shotgun sequence.
ACCESSION   DATALS010000414 DATALS010000000
VERSION     DATALS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66417"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..821)
                     /locus_tag="VHS06_09275"
     CDS             complement(99..821)
                     /locus_tag="VHS06_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014320805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX2988348.1"
                     /translation="MIRFDNVCKYYGKYCALDNISEEVFPGETLVVIGPSGSGKSTFL
                     RCINRLEEIQKGQLLVDGFDLASPKTNLTRLRAEIGMVFQHFHLYPHKTVLENITLAP
                     RLVRKMAASEAEELARELLRRVGLPDKEGAYPGQLSGGQQQRVAIARGLAMRPKIMLF
                     DEPTSALDPEMIKEVLDTMKGLAHHGMTMVVVTHEMGFAREVADRVIFMDGGRIIERG
                     TPEHFFENPQEDRTRAFLEKIL"
     gene            1074..2900
                     /locus_tag="VHS06_09280"
     CDS             1074..2900
                     /locus_tag="VHS06_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX2988349.1"
                     /translation="MTAPLLHGVLNERPAAFAADGPLAYVPGPEADHVPATYPILVYQ
                     PGASELVSLLRERRFAGWPWFRLALQAAELGLLEPPDDLLTPVHLAERWKEVGVVPYQ
                     HQLETARRVITELCGRAILADEVGLGKTIEAGIITKEYLLRRLATRILILVPAGLCRQ
                     WALELQQKFDLQPCVVRGEWDWERSDLLITSLDLAKSVRQRSRVLAQSWDLVIVDEAH
                     KLKNSRSQNWLFVSALRSKYLLMLTATPVQNDLHELYNLITLLRPGQLGTYREFRRRY
                     AAGPRQPKNAGELRELLRNVMVRNRRGDDTVRFTKRLLHSLPVELSPQEAELYRAITF
                     HLRQVARQARDVGQPMRNLLPLITLQREACSSSFAVILTLDKMRRSTSDGDLQERLTH
                     LLNLALAVSTSAKADAVEHLLATIPDKVLLFTEYRGTAAFLAQRLSNAGRTVLGFDGH
                     LSASRKDYARFLFQRSADVLISTESGGEGLNFQFCHHIVNFDLPWNPMRIEQRIGRVH
                     RLGQTEDVQVYNLATRGTVEDRLVRLLFEKLNLFSEVMGDLAELLGDDLAEGGFQQRL
                     MGAVLEADDDEDEEAGFLDLERELSGRLQSRANAGRAWWDVL"
     gene            2897..>3212
                     /locus_tag="VHS06_09285"
     CDS             2897..>3212
                     /locus_tag="VHS06_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988350.1"
                     /translation="MSGEERRTRVRSLVTGVLGAAEVPFVEADGCLSAELSREAMTAL
                     EGRWGSPATVVVTFDPETAARHPEADLIAPGSFRLDRFLAWIRREVQLSRAYLPALTR
                     DSG"
BASE COUNT          567 a         1003 c          986 g          656 t
ORIGIN      
        1 caggagtgcc agagccagac caatcaccca gagtgctgtc ttcttcaccg cttcttcctc
       61 ctccttactt cggccctacg tgtcttggat gaatgccctt agaggatctt ctccaggaag
      121 gccctggtac ggtcctcctg cggattctcg aagaagtgtt ccggagttcc ccgttcgatg
      181 atccggcctc cgtccatgaa gatcacccgg tcggcgactt ctctggcaaa ccccatctcg
      241 tgggtgacca ccaccatggt catgccgtga tgagctagtc ccttcatggt gtccaggacc
      301 tctttgatca tctccggatc tagcgccgaa gtcggctcgt caaagagcat gatcttgggc
      361 cgcatcgcca acccacgggc gatagccacc ctctgttgct ggcctccgga gagctgcccc
      421 gggtatgccc cctccttgtc cggaagcccc accctacgga gcagctccct ggcaagttcc
      481 tcggcttccg aggctgccat cttccgtacc aggcgtggcg ccagcgtgat gttctcaagt
      541 acggtcttgt ggggatagag gtggaaatgc tgaaagacca ttccgatttc cgcccgcagc
      601 cgggtcagat ttgtcttcgg agaggccaag tcgaaaccat caactaggag ctggcctttc
      661 tgaatctcct ccagccggtt gatgcagcga aggaaggtgc tcttccctga cccgctcggg
      721 ccgatcacca cgagggtttc tcccggaaac acttcctccg agatgttgtc cagagcgcag
      781 tacttgccgt aatacttgca cacgttgtca aaccggataa tcggctcgcg tgcctcgctc
      841 acaacgtacc ccccgttcct ctcccccgcc gcgacacccg tggtaggcag ccgtgtcaac
      901 cttctagcag gaaattgttc gtggttattc ttgtgggtga gaggattttc ctgctctgtg
      961 aaatggcgtt tcgcatgtat tcaggtatcc tggatacagt gcgcccggtg gcttgataag
     1021 cccagcttcc cacggacacc ataggcgcgg aggtgcccac cctggagatc attgtgaccg
     1081 cgccgctgct ccatggtgtg ctgaacgagc gccccgccgc cttcgcggcc gacgggcccc
     1141 tggcgtacgt cccggggcca gaagcggacc atgttcctgc cacttacccg atcctcgtct
     1201 accagccggg ggcgtcggag ttggtgtcac tccttcgcga gcgacggttc gccggttggc
     1261 cgtggtttcg cctcgcacta caggcggctg agctagggtt gcttgagccg cccgacgatc
     1321 tgctgacgcc agttcacctg gcggagcgtt ggaaagaggt tggggtggtg ccgtaccagc
     1381 atcagttgga gacggcgcgt cgtgtgatca cggagctgtg cgggcgagcc atcctggcag
     1441 acgaggtcgg gttgggcaag accatcgaag ctggcatcat caccaaggag tatctgttgc
     1501 gccgcttggc tacgcgaatc ctcatactcg tcccggccgg tctctgccgc cagtgggcgc
     1561 tcgaactgca acagaagttc gacctgcaac cgtgcgtggt gcggggtgaa tgggactggg
     1621 aacggagcga tctcctcatc acgtcgcttg acctggccaa gtcggtcagg cagcggtcaa
     1681 gggtgctggc tcaaagctgg gacctcgtca tcgtggacga ggcccataag ctgaagaaca
     1741 gtcgcagcca gaattggctc ttcgtgagcg ccttgcggtc gaagtatctg ctcatgctaa
     1801 ccgccacccc ggtgcagaac gacctgcacg agttgtacaa cttgattacg ctattgcggc
     1861 caggtcagct cgggacgtac cgggaattcc gccgccgcta cgcggcaggc ccccgccagc
     1921 cgaagaacgc tggggaactg cgggaactcc tgcggaacgt catggtccgc aaccgccggg
     1981 gagacgacac cgtccgtttc acgaagcggc tcttgcactc cctgccagtg gagttgagcc
     2041 cacaggaggc cgagctgtac cgagccatca ccttccacct tcggcaggta gcccgccagg
     2101 cacgcgacgt tggccagcct atgcgcaacc tgctccccct gatcacgctg cagcgggagg
     2161 cctgttcgag ttcgttcgcc gtcatcctga ctctcgacaa gatgcgccgg tctacctccg
     2221 atggggacct ccaggaaagg cttacacacc tgttaaacct ggctctggcc gtgtcgacca
     2281 gcgcgaaggc tgacgccgtg gagcatctgc tcgccacgat ccctgataag gttctcctgt
     2341 tcacggagta ccgcggcacc gcggcctttc ttgcacagcg tttatccaac gccgggcgaa
     2401 cggtcctggg cttcgatggt catctttcag ccagccggaa ggattacgcc cgattcctgt
     2461 tccagcggtc ggccgacgtc ctgatcagca cggagtcggg cggtgagggc ttgaactttc
     2521 agttctgcca ccacatcgtc aacttcgacc tgccgtggaa ccctatgcgc atcgaacaga
     2581 ggatcggcag ggtccaccgc cttggccaga cggaggatgt gcaggtctat aacctggcta
     2641 cccgcggcac ggtggaagac cggctggtgc gtctcctatt cgaaaaactg aacctgttta
     2701 gtgaggtcat gggcgacctg gccgaacttc tcggggacga cctggccgaa gggggattcc
     2761 aacaacgcct catgggtgcg gttctggaag cagacgatga cgaggacgag gaggctgggt
     2821 ttctggatct ggaacgtgag ttgagcgggc gcctgcagtc ccgcgcgaac gccgggcgag
     2881 cctggtggga cgtgctgtga gcggcgagga gcgaagaacc cgggtccgca gcctggtcac
     2941 cggggtgttg ggtgctgcag aggttccctt cgtggaggct gacgggtgtc tttcggccga
     3001 gctatcccgc gaggcgatga ccgcactgga gggacgttgg ggttccccgg caactgttgt
     3061 cgtaaccttt gacccggaga cggcggcgag acatcccgag gcggatctga tcgcaccggg
     3121 ctccttccgt ctcgaccgtt tcctggcctg gatccggcgg gaagtgcagc ttagccgtgc
     3181 ttacctcccg gcgctgacgc gcgactcggg cc
//
LOCUS       DATALS010000415         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_428297, whole genome shotgun sequence.
ACCESSION   DATALS010000415 DATALS010000000
VERSION     DATALS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428297"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /locus_tag="VHS06_09290"
     CDS             complement(<1..1653)
                     /locus_tag="VHS06_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2988351.1"
                     /translation="MPEEVFLQCTVGGPVRVYVEDGVIKRMRPLRLDDTDAASWVIEA
                     RGRSFSPPRKTTLQSYEVPERQRVYENRIQYPMKRKHFDPNGDRHTELRGKDEFVRIS
                     WDEALDLVSGEIKRIRETYGPAAITAMTSSHHNWGFLHYKLGPLGRFFNILGYSACLD
                     NPDSWEGWHWGATHAWGYWWRLGHCEQSELLQDALKNTEQIIMWSVDPNSTSYMYHGQ
                     ENLIWRHWMKELGIQFVVIDPYCNCTATTMGDKWIAPRPGTDAALAEAIGYVWLTEDT
                     YDHFFVENRTYGFDEWKKHILGETDGTPKSPKWASEICGLPASTITALAREWAAKKSM
                     LAVGSIYDANGACREAYATEWTRLCIYLMAMQGMGKPGVNFWGGTAGGAPVDYRFHMF
                     GYSDNGWDAFGNVAEKSYFPFGNSVTQKTYRILLPEIVMNPPVSWLGEGFCGNSIDQQ
                     FTKFTCPEEGPNGAPIKMIHRHGGSFISTMTDTNKWVRMYQHPNLEFVVMQDCHWQSE
                     TMFGDVLLPASTNFEHSDLSEWGAAGGYGYNDFTNNHRIVMYQ"
BASE COUNT          289 a          520 c          572 g          368 t
ORIGIN      
        1 ctggtacatg acgatgcggt ggttgttggt gaagtcgttg tagccgtaac caccggccgc
       61 accccactcg gagaggtcgg agtgctcgaa gttggtggac gccggcagga ggacgtcgcc
      121 gaacatcgtc tcgctctgcc agtggcagtc ctgcatgacc acgaactcca ggttgggatg
      181 ctgatacatc cgcacccact tgttggtgtc ggtcatggtc gagatgaagg agccgccatg
      241 gcggtggatc atcttgatgg gagcgccgtt cgggccctct tccgggcagg tgaacttggt
      301 gaactgctga tcgatggagt tgccgcagaa gccttcgccg agccagctga ccggcgggtt
      361 catgacgatc tccggcagga ggatgcggta ggtcttctgg gtgaccgagt tgccgaacgg
      421 gaagtaggat ttctccgcta cgtttccgaa cgcatcccag ccgttgtcgg agtagccgaa
      481 catgtggaag cgatagtcga ccggagctcc accggcggtg ccgccccaga agttgacgcc
      541 gggcttgccc atcccctgca tggccatgag gtagatgcag agacgggtcc actcggtggc
      601 gtaggcttcc ctgcaggcac cgttggcgtc gtagatgctg ccaaccgcca gcatgctctt
      661 cttggccgcc cattcacgcg cgagagccgt gatggtggag gcgggcaggc cgcagatctc
      721 agaggcccac ttcgggctct tgggggtacc gtcggtctcg cccaggatgt gtttcttcca
      781 ctcgtcgaaa ccgtaggtgc ggttctcgac gaagaagtgg tcgtacgtgt cctcggtgag
      841 ccacacgtag ccgatggctt cggccagcgc ggcgtccgtg cccggccgcg gcgcgatcca
      901 cttgtcgccc atggtggtgg cggtgcagtt gcagtacggg tcgatgacca cgaactggat
      961 gccgagttcc ttcatccagt ggcgccagat gaggttctcc tggccgtggt acatgtagga
     1021 ggtgctgttc gggtccaccg accacatgat gatctgctcg gtgttcttca gggcgtcctg
     1081 gagcagttcg gactgctcgc agtggcccaa ccgccaccag tagccccagg cgtgggtcgc
     1141 gccccaatgc cagccttccc agctatccgg gttatccagg caggcgctgt aaccaaggat
     1201 gttgaagaaa cgacccagcg ggcccagctt gtagtgcaga aagccccagt tgtggtgcga
     1261 ggacgtcata gcggtgatgg ccgccggacc gtaggtctcg cggatgcgct tgatctctcc
     1321 ggagacgagg tcgagcgcct cgtcccagct gatgcggacg aactcgtctt tgccgcggag
     1381 ttcggtgtgc ctgtccccat tcggatcgaa gtgcttacgc ttcatggggt actggatgcg
     1441 gttctcgtag acgcgctgtc gttccggtac ttcgtaactc tggagggtgg ttttgcgtgg
     1501 agggctgaag ctgcggcccc gagcttcgat gacccaggaa gcggcgtccg tatcatccaa
     1561 acgcagcggg cgcatgcgct tgatgacgcc gtcctccacg tacactctga cgggtccccc
     1621 caccgtgcac tgtaggaaca cttcttccgg catagttctt ctcccgatct cagacttccg
     1681 ctggctagtt agccagccct gctttgctag cgggcccccc gtgaggccgg ctggatgctg
     1741 ccactgctc
//
LOCUS       DATALS010000416         7388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_404345, whole genome shotgun sequence.
ACCESSION   DATALS010000416 DATALS010000000
VERSION     DATALS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404345"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="VHS06_09295"
     CDS             complement(<1..1746)
                     /locus_tag="VHS06_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2988352.1"
                     /translation="METSPYAAVLEGTVERVTYSNEENGYTIARLLPRGGQEVVTIVG
                     NLPSVNVGESLRLEGRWTTHPRFGRQFQVEHYQLLLPATTEGIRRYLGSGLIKGIGPV
                     TAGRIVDHFGLETLNVIEEEPERLRLVPGLGDKRAEMITRAWEAQKVIKEVMLFLQSL
                     QISSALAVRIYKHYGEASPSVVRNEPYRMAREVFGIGFLTADKIAQSVGIPRDSLERT
                     MAATLHVLSSATDEGHTYLPRQELVAKSAELLEAPTERVETAIDQLVAEQGVWSEPAE
                     EHEALYLPPLHFAEVGVCNRIKSLMEFPGDRLRQFKKVDFDVALNWLKDRDGVELSDR
                     QREAVVAALTQKVVVVTGNPGTGKTTCVRSIIRLAEAKGAEVVLAAPTGRAAKRLSEA
                     TGRPAKTIHRLLELRPGGDARYNQDDPLPADLVIVDETSMMDLLLTNSLFKAIGPESH
                     LVLAGDVDQLPSVGPGNVLRDIIDSGAVPVVRLDTIFRQAEKSSIVLNAHRINRGEMP
                     IFDRDSGESFMVQEEDPDRVLELLLELVTAHIPRRFGHHPVSGIQVLSPMYRGKVGVD
                     FLNEELQKKLNPPHQD"
     gene            complement(1844..3124)
                     /locus_tag="VHS06_09300"
     CDS             complement(1844..3124)
                     /locus_tag="VHS06_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX2988353.1"
                     /translation="MGWWFRLNITWKLLIAMILGILVGLVFPPVKDLKWMGDIFIRLI
                     QVVVVPLIFAALTVAMASIGDIRSFGRIAIKVIFFYMMTTIIAGFVGLLAANIFQPGA
                     GIDRAILGTPKAPATSAPPGLADVLLNMFPTNIVDAGAKANMIQVVVFGVLFGLIMGA
                     MGKKAQPVKDVLESLFQIMVKMVWVIMEYSPYAVFALMAWLTATTGTAALMPLIKYVL
                     TSVVALLFQTFIVVSFFVWLIARVNPIQFYRRSADYIMVAFSTRSSAASLPVSMQVAE
                     QKLGIRPEIVGFSLPLGAAMNQDGTVVWHPIAAMFIAQLYGIQLPIDQQINIVVLAVL
                     IGLGGAVIPSGGLVLLAMILQGVGLPVEGISFIAGVDAIPDMCRTTLNVVDDLSGAIM
                     VAGTEKGMLRRDILSGKRELSPDEITFTGMEQPA"
     gene            3671..4579
                     /gene="thrB"
                     /locus_tag="VHS06_09305"
     CDS             3671..4579
                     /gene="thrB"
                     /locus_tag="VHS06_09305"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX2988354.1"
                     /translation="MPKRVTVRVPATSANLGPGFDSIGIAVELYNTVEIEAADEFGVR
                     LSGEGVDVLSQGQDNLVYQGVLAVYEKLQKRVPPLVLSCENWVPLARGLGSSSAALVA
                     GLVAGNEFLGHALSNEALLQIATKMEGHPDNVTPALFGGLQVAVMNGGTISHLRVPLA
                     ADLGIIVFVPDFPMLTSEARDILPRNVSRADAIYNISRASLLIAAMATGSLGHLKAAT
                     EDALHQPHRLKLFPAMDDIIRAALDAGASGAALSGAGSSILAFSLGGEERVAEAMAEA
                     GANAEVDGRTIITKVCYSGAQIIDRG"
     gene            4589..5806
                     /locus_tag="VHS06_09310"
     CDS             4589..5806
                     /locus_tag="VHS06_09310"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX2988355.1"
                     /translation="MALIVQKYGGSSVATAERILAVARRVAATKEADNSVVVVVSAMG
                     KTTDGLIRLAQQVAPEPDERELDLLLSTGETVSCTLMAMALRGMGHTAVSLTAGQAGI
                     HTDSLHGRARIKSVDAARLQQELKDGKIVIVTGFQGITDGFDVTTLGRGGSDTTAVAL
                     AVALKADRCDIFTDVDGVYTADPRIVSQARKLDEISYQEMLEMAQLGSKVMHPRSVEL
                     GEAYGVPIMVRSSFNDEPGTLIHEVSGMETRNRVRGIAHDINVGKIAVVGVPDRPGLA
                     SSVFQPLAKAGISVDVIVQNVTVEGATDLSFTVARGDLNKAVRIIEPVAKEIGAKEVR
                     TSDLQGKVSVVGAGIQSAPGIAAATFQALYEAGINIEMITTSEIRITCIVDRDKVQDA
                     VQKLHATFELDQA"
     gene            complement(6124..>7388)
                     /locus_tag="VHS06_09315"
     CDS             complement(6124..>7388)
                     /locus_tag="VHS06_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2988356.1"
                     /translation="IHFTGGVENPLSFYFIFHVIIASILLSRGETFVQATIAILLFGG
                     LVAGEYSGAIPHITLFGPIRPDLARDGQFVGVVLLVFASTLYLAAYMATSIVKRLRER
                     DREILQLTQELQQKARELELAYNQLAELEQVKSQYMGRMSQEMKAPLTAIQSSLKVVL
                     DGLAGDVSPTQREMVTEAEKRTRALLDLVTDLLILSRSREAKLLTERRPVSPSEIVQR
                     VAENQAPRAMAKEIRFSSQVRDGIPTMFADPDAVEQLVCNLVANAVTYTPSGGRVELQ
                     VDSIGEAVRFRISDTGIGIPTADIPKIFSEFYRAENARRFSDDGTGAGPLHRAQHSRC
                     PGRRYAGRESGRHRHDLHSSSPYRQESRQPRRERRGYSAERSEGASLTEGCIHGDTSA
                     TSGNIAGECPSGNIDPRHAGGIHSQRST"
BASE COUNT         1404 a         2231 c         2337 g         1416 t
ORIGIN      
        1 atcctgatgg ggcgggttca gcttcttctg cagctcctcg ttcaggaagt ccacccccac
       61 cttaccacgg tacatggggc tcagaacctg gatcccggag accggatggt ggccgaaccg
      121 cctggggatg tgcgccgtca ccagctccag cagcagctcc agaaccctgt ctgggtcctc
      181 ctcctggacc atgaagctct caccgctgtc ccgatcgaag atcggcattt ccccacggtt
      241 gattcggtgg gcgttcagga cgatgctgct cttctctgcc tggcggaaga tggtatccag
      301 tcgcaccacc ggtaccgccc ccgagtcgat gatgtcgcgc agcacgttgc ccggccccac
      361 cgagggcagt tggtcgacgt ccccagccag caccaggtga gactccgggc cgatcgcctt
      421 gaacagggag ttggtcagca gcaggtccat catggaggtc tcgtccacga tgacgaggtc
      481 ggcgggcagc gggtcgtcct ggttgtagcg ggcatcccct ccggggcgca gctcgagcag
      541 ccggtggatc gtcttggcgg gtcggccggt cgcctccgat aatcgcttag ccgcgcgacc
      601 cgtcggcgcg gcgagcacca cctcggcgcc tttcgcctca gcaagccgga tgatggagcg
      661 tacgcaggtc gtcttgccgg tgcctgggtt gccggtgaca acgaccacct tctgtgtaag
      721 cgccgccacc acggcctccc gctgccggtc ggatagctcc accccgtcgc gatccttcag
      781 ccagttgagt gcgacgtcga aatcaacctt cttgaactgc cgtagccggt caccggggaa
      841 ctccatgagc gacttgatcc gattgcacac cccgacttcg gcgaagtgga gggggggcag
      901 gtagagcgcc tcgtgctcct cggcgggctc gctccacaca ccctgttcgg cgacgagttg
      961 atcgatagcc gtctccaccc gttccgtggg agcctccagc agttcggcgc tcttggccac
     1021 gagttcctgc cgaggcaaat aggtgtgccc ctcgtccgtg gctgaggaga ggacgtgcag
     1081 cgtcgcagcc atggttcgct ccagggagtc gcgaggtatc ccgacgctct gggcgatctt
     1141 gtccgccgtt aggaagccga tgccgaacac ctcccgcgcc atgcggtaag gctcgttccg
     1201 gacgacggag ggggatgcct ccccgtagtg cttgtagatg cgcaccgcga gagcgctgga
     1261 gatctgcagc gactggagga agagcattac ctccttgatc accttctggg cctcccaggc
     1321 gcgggtgatc atctctgccc gcttatcgcc cagcccgggc accagcctca atcgctccgg
     1381 ttcctcctcg atcacattga gggtctccag cccgaagtgg tccacgatcc ggccggcggt
     1441 caccggtccg atacctttga tcaacccgga gccgaggtag cgtcggatgc cctcggtggt
     1501 ggcgggaagc agtagctggt aatgctctac ctggaactgc cgtccgaagc gggggtgggt
     1561 ggtccaacgc ccttcgagcc gcagagactc gccgacgttg accgagggga ggttccccac
     1621 gatggtcaca acttcctggc cgcctcgagg cagtagcctg gcgatggtgt agccgttctc
     1681 ttcgttgctg taggttaccc gctcgaccgt tccctcgagg acggcagcgt agggggatgt
     1741 ttccataaat cgcagtacct ggaggaatta taccaggaga gatgtgatgg gggccgcgtg
     1801 agcgcggccc ccatcacgtt tgggacacag ttgcacgccg gcttcaggct ggctgttcca
     1861 tgcctgtgaa ggtgatctca tcgggcgaca gttcccgttt gccggacagg atatccctgc
     1921 gcagcatgcc cttctcggtg ccggccacca tgatcgctcc agagaggtcg tcgaccacgt
     1981 tgagggtagt ccggcacata tccggtatgg cgtccacgcc cgcgatgaag gagatcccct
     2041 ccacgggcag cccgaccccc tgcaggatca tcgccagcag cacgaggcct ccgctgggta
     2101 tcaccgcgcc acccagtccg atcaacactg caagcaccac gatgttgatc tgctgatcga
     2161 tggggagttg aatgccgtag agctgggcga taaacatcgc ggcaatgggg tgccacacca
     2221 ccgtgccgtc ctggttcatc gccgccccca gcggcaggga gaagccgacg atctcggggc
     2281 ggatgcccag cttctgttcg gctacctgca tggagacggg cagcgaggcg gctgatgatc
     2341 gggtgctgaa ggccaccatg atgtagtcgg ccgatcgcct gtagaactgg atcgggttga
     2401 cgcgggcgat caaccagacg aagaaggaga ccacgatgaa ggtctggaac agcaacgcca
     2461 cgacggatgt gagcacgtac ttgatcagcg gcatcagcgc ggcagtgccc gtggtggcgg
     2521 tgagccatgc catcagggcg aagacggcgt atggggagta ctccatgatc acccagacca
     2581 tcttcaccat gatctggaac agcgactcca gcacgtcctt caccggttgg gccttcttgc
     2641 ccatcgcacc catgatcagg ccgaacagta ctccgaacac caccacctgg atcatgttcg
     2701 ccttggcgcc ggcgtcgacg atgttggtgg ggaacatgtt gagcagtaca tcggccagac
     2761 cggggggtgc ggaggtggct ggggccttcg gtgtgccgag gatggccctg tcgatcccgg
     2821 cgccaggctg gaagatgttg gcagcgagga gacccacgaa cccggctatg atggtggtca
     2881 tcatgtagaa gaagatcacc ttgatcgcta tgcggccgaa ggagcgaatg tcgccgatgc
     2941 tggccatcgc gacggtgagg gcggcgaaga tcagggggac caccaccacc tgaatcagcc
     3001 tgatgaagat gtcgcccatc cacttcagat cctttactgg cgggaacacc agccccacca
     3061 gtatgccaag gatcatggcg atcagcagct tccaggtgat gttgagacgg aaccaccaac
     3121 ccattgcgaa cctccatact ctcagcaacg cacgcctggc cgccatgtct atacatgggg
     3181 acaggctcga aaagtgaagg aactcgacga tggatgatgg attgagggga tgtgccggag
     3241 gccgcgcgga cttacggtcc gacacggatt ccagcaacta ccactgggga tcggccaccc
     3301 tggatcgatg caccccttcc cacttcacgc ccggggcgct cctccccggg gctgggagcg
     3361 gtgatttgcc gctcgtgctg gccgacacat ttagctatcg agatgggaac cgcgacgcag
     3421 ggccaatcag tggaaccgca atcgcccatc tcagcaccat attacacctc gcagaggcta
     3481 agtcaatgaa gaggccatgg gcagcggccc tttggctcgg cgcccctcga cattccgcgt
     3541 ttagtggcga caggacgcca tggggttttg gtgcacttgt caaggaattg gacagtggtg
     3601 gcggggtgca gtattatgtt cgccgcgcct tttgccaatt cagcttgcgg cggagttgtt
     3661 aatccccatt atgcccaaac gagtcaccgt tcgagttcct gctaccagcg ccaacctcgg
     3721 gcctggtttc gattccatcg gaatagcagt agaactgtac aatactgttg agatcgaggc
     3781 ggccgacgag ttcggcgtgc gtctttcggg tgagggagtc gatgtgctct ctcagggcca
     3841 ggacaacctg gtatatcagg gggttctggc agtctacgag aagcttcaga agcgagttcc
     3901 cccgctagtt ctgtcttgtg agaactgggt gcccctggcc cgggggcttg gaagcagtag
     3961 cgcggcattg gttgccggcc tggtggccgg aaacgagttt cttggacacg ccctctccaa
     4021 tgaggcgttg cttcagatag cgacgaagat ggagggtcac cccgacaacg tcacaccagc
     4081 ccttttcggg gggctgcagg tggcggtgat gaacggggga accatctccc acctgcgggt
     4141 tccgctggcc gccgacctgg ggataatcgt ctttgtcccc gattttccga tgctcacctc
     4201 agaggctcgg gatattctcc ctcggaacgt gtcacgagcg gatgccattt acaatataag
     4261 ccgtgcgtcg ttgctaatcg cggcgatggc cacgggttcc ctgggccacc tgaaagccgc
     4321 cactgaagat gccctgcatc agccgcacag gctcaagctt ttccccgcca tggacgacat
     4381 cattcgggca gccctggatg ccggtgcctc gggagcagca ttgagcggtg ccggttccag
     4441 tatccttgcc ttctctctgg gaggtgagga gcgggtagcc gaggcgatgg ccgaagcagg
     4501 cgcgaacgcc gaggtcgacg gcaggaccat cattaccaag gtgtgctact ccggggcgca
     4561 gatcatcgac agaggttagg ggactgcaat ggcattgatc gtccagaagt acggcggctc
     4621 ctcggtcgcc accgccgaac gaatacttgc ggtcgcccgc agggttgcgg ccacgaaaga
     4681 agcggacaac agcgtcgtgg tggtcgtctc tgccatgggc aagaccaccg atgggttgat
     4741 tcgcctcgcg cagcaagtag cgcccgagcc ggatgagcgg gagttggacc tgcttctatc
     4801 cacgggtgaa accgtctcct gcaccctcat ggcgatggct cttcgtggga tgggacacac
     4861 ggcggtttct ctgaccgccg gccaggcagg gatccacaca gactctctcc acggaagggc
     4921 tcgtatcaaa tcggtggacg ccgcccgcct tcagcaggag ttgaaggacg gcaaaatcgt
     4981 tatagtcacc ggcttccagg ggatcacgga tgggttcgat gtgaccaccc tgggccgcgg
     5041 cggctccgat accacggcgg tggctttggc cgttgcgctc aaggcggatc gctgtgacat
     5101 attcaccgac gtggacggcg tctacactgc cgacccgagg atcgtttctc aggcccgcaa
     5161 gctggacgag atctcctatc aggagatgct ggagatggct cagctgggct ccaaggtgat
     5221 gcatccccgg tctgtggagt tgggggaggc ctacggcgtt cccatcatgg tccggtctag
     5281 tttcaacgat gagccgggca ccctgattca tgaggttagt ggaatggaaa ccagaaaccg
     5341 agtgcgcggc atagctcacg acatcaacgt tggcaagatc gcagtggtgg gggttccgga
     5401 tcggccggga ttggcgtcgt cggtgttcca gccgctggcc aaggcgggga taagcgtcga
     5461 cgtgatcgtg cagaacgtca ccgtcgaggg cgccacggac ctctccttta cggtggcccg
     5521 gggagacttg aacaaggcgg ttcggatcat cgaaccggtg gccaaggaga tcggggcgaa
     5581 ggaggtgcgt acttccgacc tgcaggggaa ggtctcggtg gtcggtgccg gcatccagag
     5641 cgcacccggc atcgcggcgg cgaccttcca ggcgctgtac gaggcgggga tcaacatcga
     5701 gatgatcacg acgtccgaga tccggatcac ctgcatcgta gatagggaca aggtacagga
     5761 tgcggtgcag aagctgcacg ccaccttcga gttggaccag gcataagaga ggattcgcag
     5821 agtagacgag gcgggccgga aaaccggccc gccttttcga ttctcgcgca tgtcgtgggg
     5881 agtagggcgg ggcgctctgc cccgccgcaa tcgcttggag acagccaccg agtggcaggg
     5941 cacaaggctc tatcctacac cgggttcacc tactagaggt cgtcttcgct gttgctgatt
     6001 gagccggcta tcgattctag caaccgagca accacggcac aggagaccgc gccgaagcct
     6061 cccaccagtg caatggcgag gccgtttcgg gaggaggccg actcccagag gcccgcaaag
     6121 agctcaggtc gagcgctgag agtgaatccc accagcatgg cgaggatcga tattcccaga
     6181 aggacattcg ccagcaatat tgccgctcgt agcgctcgtg tcgccatgga tgcatccctc
     6241 tgtcagggaa gcgccctcgc ttctctctgc tgagtatccc cgccgctctc tccgtggttg
     6301 tcgggactct tgccgatagg gagaactact gtgaaggtcg tgccgatgcc tacctgactc
     6361 tctacctgca tatctccgcc cagggcatcg actgtgctgc gcacgatgga gaggcccagc
     6421 ccccgtgccg tcgtcactga agcgccgtgc attctcggct cgatagaact cgctgaagat
     6481 tttgggtatg tcggcggtcg ggatccctat gccggtatcg ctaatccgga aacgcactgc
     6541 ctctccgatg gagtcgacct gcagctcaac acggcccccg ctgggagtgt acgttaccgc
     6601 gttggccacc aggttgcaga ccagctgttc taccgcatcg gggtcggcaa acatggtggg
     6661 gatgccatct cggacttggg aggagaagcg aatctccttg gccatcgccc tgggagcctg
     6721 attctccgcg acccgctgaa ctatctcgct tgggctcacc ggtcgccgtt cggtaagcaa
     6781 cttggcctcc ctcgaccgag aaaggatgag gagatcggtg accaggtcca ggagcgccct
     6841 agtgcgcttc tctgcctcgg tgaccatctc cctttgtgtg ggggacacgt cgccggccag
     6901 accgtccagc acgaccttga gagagctttg aatggcggtg aggggcgctt tcatctcctg
     6961 agacattcga cccatgtatt ggctcttcac ctgctccaac tctgccagct ggttgtaggc
     7021 tagctccaac tctcgagcct tctgttgaag ctcctgggta agctgcaata tctccctgtc
     7081 ccgctccctc aacctcttca ctatcgacgt ggccatgtag gcggccagat agagggtgct
     7141 ggcgaacacc agaagcacaa cccccacgaa ctggccgtct cgtgccaggt cggggcggat
     7201 cgggccgaag agggtgatgt gggggatcgc gcctgagtac tcgcccgcca ccagcccgcc
     7261 gaagagcaag atcgctatag tggcctggac gaaggtctct cccctggaga gcagaatgct
     7321 ggcaatgatg acgtggaaga tgaagtagaa ggaaagaggg ttctccacgc cacccgtgaa
     7381 atgtatta
//
LOCUS       DATALS010000417         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_113067, whole genome shotgun sequence.
ACCESSION   DATALS010000417 DATALS010000000
VERSION     DATALS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113067"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VHS06_09320"
     CDS             <1..864
                     /locus_tag="VHS06_09320"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HEX2988357.1"
                     /translation="LPILKSTYPEASQELITGRDNSILFTGFKVDRAVATRDQGLLKR
                     CGGTEQLEIDPSSLDQMRCASTESVEWTGSLFVDRSGHYRLTVEGRAAEIYIDGDGLS
                     SAGRSLAQGWHTISLHAQPGSSSSHMALKWQRDGQPLQTVQGQYLQSRALPGTIRRKV
                     VAADGRAEESLDRAIGFRNLGDATTVRPPATFVWNATLNVPATGRYLFELRSDGEAVL
                     SVADRVVAAISGKGTSLRNEARALELPAGPKEFEIKYHWQNGVMGMVEALWTPPGGAQ
                     SIIPPSALSRP"
     gene            937..>2432
                     /locus_tag="VHS06_09325"
     CDS             937..>2432
                     /locus_tag="VHS06_09325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2988358.1"
                     /translation="MNSTQYERGVTPRPSFVSSLYNSSSARYGLVAIMALALFFRLFG
                     LNWDSNQHLHPDERHIVMVTMDRVSLPFPNLADAHLGDVKHSTLNPRSVDPINGQPRS
                     FAYGSLPVYLLKLVGHVVSPWWPPANGIDGLTLIGRILSALFDLGTVLLVFFIGRRVY
                     GVAAGLVASLLVSLTVMDIQLAHFYASDTLLTFFVLLTILAALRAHERPSTGRVVAMG
                     VAAGLAISCKVSAAPVLAACVLACLFARDRIAGRSEGTSATSQAFSLAALSVVMAGLV
                     FFIFQPYVLLDPQSFIKDIKYESEMVRGIQIPPYTIQYIGTVPIVYQIGNLFAWVAGP
                     ALTIVLLVGLLLFVVRLLARRRGEEWLLAAWIFAYLAATLGFQVKFVRYMLPVLPLLV
                     LLSVAWLLPRGLGDLARAARPSRWATVVLAIVVAGTALQAVAFTSIYQQEHSRVQASR
                     WIYANVPKGAALSAEHWDDSLPLGLADVPAKTSDYRLVTFPMYDTDNA"
BASE COUNT          445 a          763 c          713 g          511 t
ORIGIN      
        1 ttgcctatcc tcaagtcgac ctaccctgaa gctagccagg aactgatcac tggccgcgat
       61 aactcgatcc tgtttacggg attcaaggtt gatagggcag tggcaactcg cgaccaaggt
      121 ctcctgaaga gatgtggcgg gaccgaacaa ttggagatcg accccagttc attggaccag
      181 atgagatgcg cgagcaccga gagtgtagag tggactggaa gccttttcgt cgatcgttcg
      241 ggccactatc gactgaccgt cgagggccga gccgctgaga tctacatcga tggtgacgga
      301 ctgtcgtccg ctggaagatc attggcgcag gggtggcata cgatatctct gcatgcccag
      361 ccgggcagtt catcgagcca catggcgcta aagtggcagc gagacggcca gccgcttcaa
      421 accgtgcagg ggcaatacct gcagtccagg gctctacccg ggaccattcg tcgcaaggta
      481 gtggctgcgg acggcagggc agaggagagc ctggaccgag ctatcggctt tcgcaatctc
      541 ggtgatgcca cgacagtccg cccccctgcc accttcgtct ggaacgcaac tctcaacgtg
      601 cccgcaaccg gccggtacct gttcgagttg cgctcggacg gcgaagcagt actctcggtg
      661 gccgacaggg tcgttgccgc gatcagtggg aaaggcacat cactcaggaa cgaagctcga
      721 gcgcttgaat tgccggcagg ccccaaggag ttcgagatca agtaccactg gcagaacgga
      781 gtcatgggaa tggtagaagc gttgtggaca ccgccgggtg gagcgcaatc gataatccct
      841 ccatctgccc tttcgaggcc atgatgtgtc gctgagagta caccaggaaa cctctcctat
      901 tgcggcaatg gctgtggcct ctggtacaat agcgccatga atagcaccca atacgagcgg
      961 ggagtgactc cccgcccctc atttgtgtct tcgctctaca attccagcag tgcaaggtac
     1021 ggcctggtcg ccataatggc gttggccctc ttctttcgtc tcttcggcct gaactgggat
     1081 tccaaccagc acctgcaccc ggatgagcgt cacatcgtga tggtcaccat ggaccgggtg
     1141 tcactgccat tccctaacct ggccgacgcc cacttaggag atgtcaagca cagcaccctg
     1201 aacccgcgct ccgtggaccc gatcaacggc cagcccagga gctttgccta cggctccctc
     1261 ccagtctacc tgctgaaact ggttgggcac gtcgtctccc cctggtggcc gccggccaac
     1321 ggcatcgacg ggctaacgct catcggccgc atcctttccg ccctcttcga tctcggcacc
     1381 gtcctgctgg tgttcttcat tggtaggagg gtgtacggcg tggctgccgg actagtcgca
     1441 tcgctgttag tgtccctgac cgtgatggac attcagcttg cccacttcta cgcatccgac
     1501 acgctgctta ccttcttcgt tctgctcacc attctcgcgg cgctccgagc ccacgagcgt
     1561 ccatccacgg gaagggttgt ggcgatgggc gttgccgcgg ggttggccat ctcatgcaag
     1621 gtatcggcgg cgcctgtcct ggcggcctgc gtcctggcct gcctgttcgc ccgggatcgt
     1681 atagccggac gaagcgaggg tacatcagcc acgagccagg cattctcgct agcggcactc
     1741 tcggtagtga tggcagggct ggtgttcttc atcttccagc cctacgtcct cctggaccct
     1801 cagtctttca tcaaggacat caagtacgag agcgagatgg tgcgtgggat ccagatacct
     1861 ccatacacca tccagtacat cgggaccgtc ccgatcgtgt accagatcgg caaccttttt
     1921 gcctgggtcg cgggccccgc actgaccatc gtgttgctcg tgggactcct cctgtttgta
     1981 gtccggttgc tggctcggcg tcggggcgag gagtggctgc tggccgcgtg gatcttcgcc
     2041 tacctggcgg ccacccttgg attccaggtc aagttcgtgc gatacatgtt gccggtgctt
     2101 ccgctgctcg tcctattgag cgttgcatgg ttacttccgc gcggcctggg tgatcttgca
     2161 cgtgcggcac gcccctccag atgggcaacc gtagtgctgg cgatagtcgt tgccgggacg
     2221 gcacttcagg cggtcgcttt cacctccatc taccagcagg aacactcacg ggtacaggcc
     2281 agtcggtgga tatacgccaa cgttcccaag ggcgctgccc tgtccgccga gcactgggac
     2341 gattcccttc ccctcggcct ggccgacgtc ccggccaaga cgagcgacta ccgtcttgtg
     2401 accttcccga tgtacgacac cgacaacgcc ca
//
LOCUS       DATALS010000418         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_113105, whole genome shotgun sequence.
ACCESSION   DATALS010000418 DATALS010000000
VERSION     DATALS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113105"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /gene="der"
                     /locus_tag="VHS06_09330"
     CDS             <1..1270
                     /gene="der"
                     /locus_tag="VHS06_09330"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX2988359.1"
                     /translation="TLFNRLIGERRAIVDDTPGTTRDRIYGTVEWAGRAFTLVDTGGL
                     SFEDVDQVNQGIVSQAEEAIERADVILFVVDAREGPSAIDYDVAELLRRSDKPVILVA
                     NKADNPERMRNAVEFYQLGMGEPMAISSVHGHGTGDLLDEIVKDFAEGEVEEPGEEVR
                     LAIVGRPNVGKSSLVNAIAGQSRSMVSDIPGTTRDVIDTVVQYHGKSILLVDTAGIRK
                     RGKVEQGIEKYSVIRAVQAVERSDVAALIIDASEGLTAQDTHVAGYILDAAKGMMVVA
                     NKWDLVTKSPTIMQEYTVALRQALKFADYVPIIFTSALTKQRVPKLLDTALQIAQERE
                     KRIPTAALNDAMQEAFVHHSPPVSRGRALKLLYVTQADVKPPTFVFFVNDPRLLHFSY
                     QRYLENEIRSRWSFTGTPIRMVFKPRSVRE"
     gene            1422..>1839
                     /locus_tag="VHS06_09335"
     CDS             1422..>1839
                     /locus_tag="VHS06_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988360.1"
                     /translation="MPIEGWNWWGSGALSEAMEGEETGGIMAFTPAESKRLIGKAIAA
                     MPEVKGALEKGRVVVTGGTTNAYVAEEILGKPVAPEYFIKGNITHGLACSTSRSDSWI
                     KPFILIDGKPVDRELNEVLMEFDGGDVYIKGANAVDA"
BASE COUNT          377 a          498 c          626 g          338 t
ORIGIN      
        1 cacccttttc aacagactga tcggcgaacg tcgcgccata gtcgacgaca cgcctggaac
       61 cacccgagat cgcatctacg gcaccgtgga gtgggcgggg cgcgccttca ctctggtgga
      121 cacggggggg ctctccttcg aggatgtcga tcaggtgaat caggggatag tcagccaggc
      181 cgaggaggcg atagagaggg cagacgtgat cctctttgtc gtggatgctc gggagggccc
      241 atccgcaatc gactacgacg tagcggagtt gctgaggcga tccgacaagc cggtgatcct
      301 tgtggccaac aaggccgata acccggagcg gatgcggaat gccgtggagt tctatcagtt
      361 gggcatgggc gagccgatgg ccatttcctc cgttcacggc cacggaaccg gcgatctcct
      421 ggacgagatc gtcaaggatt tcgccgaggg ggaggtggag gagccggggg aagaggttcg
      481 gctggccatc gtcggcaggc cgaatgtcgg caagtcctct ctggtgaacg ccattgccgg
      541 gcagtctcgg tcgatggtaa gcgatatccc cggcaccacc cgcgacgtta tcgacaccgt
      601 ggtccagtat catggcaagt cgatactgct ggtggacacg gcggggattc ggaagcgtgg
      661 caaggtggag caggggatcg agaagtacag tgtgatccga gcggtccagg cggtggagcg
      721 ctcggatgtc gccgcgctga tcatagacgc gtctgagggg ttgaccgccc aggacaccca
      781 cgtggcgggt tacattctgg atgcggccaa ggggatgatg gtggtggcga acaagtggga
      841 tctcgtgacc aagagcccca ccatcatgca ggagtacacc gttgcactca ggcaggcgct
      901 gaaattcgcc gactatgtgc cgattatctt tacctctgcc ctgaccaagc agcgcgtgcc
      961 gaagctgttg gacacagcgc tgcagattgc ccaggaacgg gagaagcgga taccgacggc
     1021 ggcattgaac gacgcgatgc aggaggcttt cgttcaccac tcgccccccg tgtcgcgtgg
     1081 cagggcgctg aagctactgt acgtcacgca ggcggacgtg aagccgccga ctttcgtttt
     1141 cttcgtgaac gatccacggc tgctccactt ctcctatcag cgatatctgg agaacgagat
     1201 caggtcgcgc tggagtttca cgggaacgcc catccgcatg gtgtttaagc cacgctccgt
     1261 acgggagtga cactcgccgg cgtatccatt ggcggaggga agctacctta gccgcaaagg
     1321 tcggagacgc cgagtgaggt tccagtagct actgccttcg acttctcgcg ttatagcagt
     1381 tttgttgttc aacttagaag gagactgttg ggaggagaaa gatgcccata gaagggtgga
     1441 actggtgggg cagcggtgcc ttatcggagg cgatggaggg cgaggagacc ggcgggatca
     1501 tggccttcac gcctgccgag tccaagagac tgatcggcaa ggcgattgcg gcaatgccgg
     1561 aggtcaaggg tgccctggag aagggccggg tggtggtgac cggcgggacg actaatgcct
     1621 acgtggccga ggagatcctg ggcaagccgg tcgctcctga gtacttcatc aaggggaaca
     1681 taactcacgg gctggcctgc tccacctccc ggagcgactc ctggatcaaa cccttcatcc
     1741 tgatcgacgg gaagccggtg gaccgtgagc tcaacgaagt gctgatggag ttcgacggag
     1801 gtgacgtcta catcaagggc gccaacgccg tcgacgccg
//
LOCUS       DATALS010000419         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_66926,
            whole genome shotgun sequence.
ACCESSION   DATALS010000419 DATALS010000000
VERSION     DATALS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66926"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VHS06_09340"
     CDS             complement(<1..643)
                     /locus_tag="VHS06_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2988361.1"
                     /translation="MRRDAARGDRLMRVGELAEATGVSVQTIHFYLREGLLARPIKTS
                     PNMAYYGPEFVEEIRLIKELQEKRYLPLSLIRLVLEAKRQGQEVGHLQEMRLSLEELF
                     RPRGPEEKMEPVSTVELVAMTGLSLQALADLEQISLLMPSPAPEDKRYDGLDLHIARS
                     AKKLLDLGLAPSDLSFYSQYVEALRSESRVMHDKVMHNHTAGMPPIPLKELKEL"
     gene            883..2124
                     /locus_tag="VHS06_09345"
     CDS             883..2124
                     /locus_tag="VHS06_09345"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2988362.1"
                     /translation="MEGEERSELQPAEPQPEEPEEKEDPAQQPADPEVVAGGAGSFDS
                     ASDPGVTAQFAAVSRKRAAEPPRPAAEAESGHATEESSDPGAEAAPTLSPALEAEVAR
                     STPPSFADNQGLFTAVPSAGGKVGLGATALLAVCAVTALAYAAGRPIAIDSYFGWVAG
                     LAALLLAVAAGSLTYGWRSMRYRLEDQKLTISWLWIKETVPLGRVDGVFGGRRFGKTI
                     DVDGLAWPGHYVGYAAAEDLGKVKFYGTSRDPSTAIVIATAKACYAITPADLDGFRSR
                     LISRLEALTPEDMARSREPRTAMPWLFSFSILRDGMALGLLGVALLVLLVSFGYVSAR
                     FPTLPELMPLHFNFANEPDLIGPPRDAFRMPGIGAFILLVNLVVATALHRGRREASRI
                     LAGATPFIQLVMLIAILRVVH"
BASE COUNT          360 a          672 c          737 g          468 t
ORIGIN      
        1 gcagttcctt gagctctttg agggggatgg gcggcatccc cgcagtgtgg ttgtgcatca
       61 ctttgtcgtg catcacccta gactcggagc gcagcgcctc gacgtactga ctgtagaagc
      121 tcaggtcgga cggggcgagc cctagatcca gcagcttctt ggcggagcgg gcgatgtgca
      181 ggtccaggcc gtcgtagcgc ttgtcctcgg gagccggaga gggcatcaac aggctgatct
      241 gctctaggtc ggccagggcc tgcaatgaga gtcccgtcat ggccaccagc tcgacggtgg
      301 agacgggctc catcttctcc tccggccccc tggggcggaa cagctcttcc agggagagac
      361 gcatctcctg gaggtggccc acctcctgtc cctgccgttt ggcctccagc accagccgga
      421 tgagggagag gggaaggtag cgtttctcct ggagctcctt gatgaggcgg atctcctcga
      481 caaactcggg accgtagtag gccatgttgg gggaggtctt gatgggacgg gcgagcaatc
      541 cctcgcggag gtagaagtgt atggtctgga ctgagacgcc ggttgcctct gcaagctcgc
      601 cgacgcgcat cagcctgtct ccgcgggctg cgtctcgtct catgtcgtcc cccctcctcc
      661 tccgtatcga gtagtgtgaa gtagcatata gttatactat gtacctgtca actgtttctt
      721 gaccggggtg ctgcgctctg ctatattcag ccgagttaca taactatagg gtgctggcca
      781 ggcatccggc gagtctatcc gatattccct ggaaacgatt gagaggcggg gtcaggactg
      841 gccccgcctc tcctgctata tgagtctctg gaggggccgg gattggaagg cgaagagaga
      901 agcgaactgc aaccggcaga gccacagcct gaagagcctg aagagaaaga ggatcctgcg
      961 cagcaaccgg ccgacccaga ggtcgtggcc ggtggtgccg gctccttcga tagtgcatcc
     1021 gatcccgggg tgacggcgca gttcgctgcg gtttcccgga agagggctgc ggagcctcca
     1081 cgccctgcgg ccgaggcaga gtctggccat gccaccgagg aatcgtccga tcctggggcg
     1141 gaggcggctc ccaccctatc gcctgctttg gaggccgagg tcgcccgctc cacgcctccc
     1201 tcctttgcgg ataaccaggg cttgtttacg gccgtcccat cggccggagg caaggtaggg
     1261 ctgggggcga cggcgctgct ggccgtctgt gccgtgacgg cgctggcgta cgccgcggga
     1321 cgccccatcg cgatcgacag ctacttcggc tgggtggcgg ggctggccgc cctgctgctt
     1381 gcggttgcag ccggtagcct cacctacggc tggcgctcca tgcgttaccg gttggaggac
     1441 cagaagctca ccatctcctg gctctggatc aaggaaacgg tcccgctggg gcgggtggac
     1501 ggcgtcttcg gcggccgccg tttcggcaag accatcgacg tggacggact ggcctggccg
     1561 ggacactacg tcggctacgc cgccgcggag gatctgggta aggtcaagtt ctatggtacc
     1621 agccgggatc catccacggc gatcgtcatc gccactgcca aggcctgcta cgccatcacg
     1681 ccggccgacc tggacggctt ccgatccagg ctgatctctc gtttggaggc gttaacccct
     1741 gaggatatgg cgcggtctcg ggagccgcga actgctatgc cctggctgtt ctccttctcc
     1801 atcctgcggg acgggatggc gttgggtctg ttgggcgtgg cgttgcttgt cctgctggtg
     1861 tccttcggct acgtgagcgc tcgcttcccc acgctgccgg agttgatgcc gctgcatttc
     1921 aactttgcca atgaaccgga tttgattggc cctccccggg atgccttccg catgccgggc
     1981 atcggagcgt tcatcctgct ggtcaacctg gtggtggcga ccgcacttca tcgcgggcgg
     2041 cgcgaggcct ccaggatact cgcgggtgca acgcccttca tccagttggt gatgctgatc
     2101 gcgatactgc gagtggtaca ctagctgcat cttattgctt cctgcttgac cggttcattg
     2161 cttcgagcgg aggtaacgga tagttgtacg agccacctca gggtgatgtc gccttatata
     2221 ttgattggga gaacatc
//
LOCUS       DATALS010000420         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_339748, whole genome shotgun sequence.
ACCESSION   DATALS010000420 DATALS010000000
VERSION     DATALS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339748"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..531)
                     /locus_tag="VHS06_09350"
     CDS             complement(142..531)
                     /locus_tag="VHS06_09350"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2988363.1"
                     /translation="MGNSPKEPFVIHVRDVKPFVAQSPDHRELRVMLSPKRDGVAPGL
                     AFGVVEVKPGPTPPPHSHATTQEAWYFLSGKGQIKVGEKVIEVEPGTVVVSPPQVDHQ
                     LINTGTEVLRALFIFTPSGDEVPLIVE"
     gene            complement(897..>2126)
                     /locus_tag="VHS06_09355"
     CDS             complement(897..>2126)
                     /locus_tag="VHS06_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX2988364.1"
                     /translation="SMRAAFSGQGAGTAMLLVRLDDRADLAAKAAEIRSAVEEIQGDY
                     RISVSNEQSSGTARVQLAVSGQDSGAVDRMAGEVESALQNTQGLANLSTDRATSAPEI
                     SIQVDPSKAAELGLTTAQVAAGVRGMVAGQSVGRIEPQGGESMDLRLTVAPDAVNSPD
                     QLGRLPFGSPKSVPLSDMAQIKQEPRPVQITRVAQNPAVNVTADVIAGDTGAVSKEIR
                     KRISELPAEPGVSVDYGGVLQQLSEGFSSMGKGQMVGVGLVFLVMALFFNSVLDPLVI
                     LLSLPLAAVGALPALYLAGKTLSMSVMIGMLMLIGIVVTNAIVLLDFARRAMREGRDP
                     RAALMDAGQVRLRPILMTALTTILALMPLALGLEKGSIIAAELATVVIGGLLSSTMLT
                     LVVVPVVYSLVHRRAST"
BASE COUNT          443 a          714 c          602 g          367 t
ORIGIN      
        1 cttctttaag tcgtatgccc cggctactcc tggatcgcag tgctactgcc gggtgtcatc
       61 ctgaacggag tggaggatct ctccgtccgc gaggagatcc ttcgccggcg ctcaggatga
      121 caaatccgcg gcggtcgatg actactccac gatgagcgga acctcgtcac cgctcggcgt
      181 gaagatgaag agtgccctga gcacctcggt accggtgttg atcagttgat gatccacctg
      241 cgggggagac acgacgacgg tccccggctc cacttcgatc accttctcgc caaccttgat
      301 ctgccccttg cccgatagga agtaccaggc ttcctgggta gtagcatggg agtgaggcgg
      361 aggcgtgggt ccgggcttca cctccacaac accgaaagcc agacccgggg caacgccgtc
      421 acgcttgggc gaaagcatca ccctcaactc acggtgatcc ggagactgag cgacaaacgg
      481 cttcacgtcc cgcacgtgaa taacaaaagg ctccttagga ctattcccca ttgaatgacc
      541 tccctgcgac tgcgtcgaag tagacgaaag cgtactcata gtagtggaga ggtggggaga
      601 gatcaagcag caggacaagc agtaggaagg gaggctgggc ttctcgtggc aaggcaaacg
      661 ctccgaggag agccatcacg aatcggcaac acggctcgga ggccgagatc gagggtaggg
      721 ccgatagggg ccacgggctg gggtagaagg tcgaaaagtc taaccaacga ccccgacgcc
      781 taactcccgt cccccgctcc caaacccttc gctggaagcc gactattgat gactgaccac
      841 ggagcgctta caccgataat gatcggcaag agaccgaaga actttagccc ccgcgatcaa
      901 gtgctggccc ggcggtgaac aagactgtac accacgggca ccaccaccag cgtcagcatg
      961 gtagagctga ggagtcctcc gatcacgacc gtggccagtt cagccgcgat gatcgagccc
     1021 ttttccagtc ccaatgccag gggcatcagc gcaaggatcg tggtaagagc cgtcatcagg
     1081 atcggtctca gccgaacttg ccctgcatcc atgagcgcgg cacgaggatc ccttccctcc
     1141 ctcatcgccc gcctggcgaa atccagaagc actatagcgt tggtcactac gatcccgatc
     1201 agcatcagca tgccgatcat cacggacatg ctgagcgtct tcccggctaa gtagagggca
     1261 ggcagcgctc cgaccgccgc cagcggcagc gacagcagga tcaccagcgg gtccagcacc
     1321 gagttgaaga acagcgccat caccaggaac accagcccca cccccaccat ctgccccttt
     1381 cccatggagc tgaagccctc tgatagctgc tgcaagaccc caccgtaatc aactgaaaca
     1441 ccgggctccg cgggaagctc cgagatccgc ttcctgatct ccttggacac cgcgccggta
     1501 tcccccgcaa tgacgtcggc agtcacgttc accgccgggt tctgcgcgac ccgcgtgatc
     1561 tgcaccggcc ggggctcctg cttaatctgc gccatatcag aaagcggcac gctcttcgga
     1621 ctgccgaagg gcagtctgcc cagttgatcc ggactgttca cggcgtcggg agcgacggta
     1681 agccggagat ccatggattc accaccctgt ggctcgatcc tcccaaccga ctgtccggcc
     1741 accatacccc tgacccccgc ggccacctgc gcggtggtca gccccagctc ggccgccttg
     1801 ctgggatcca cctggataga aatctccggc gctgaggtgg cgcgatcggt cgagaggttg
     1861 gccaatccct gcgtgttctg aagagccgat tccacttctc ccgccattcg gtccaccgcg
     1921 cccgagtcct gccccgagac cgccaactgc accctcgcgg ttccagagga ctgctcgttg
     1981 gacacgctga tacggtagtc tccctggatc tcctcgacgg ccgaccttat ctcggcggct
     2041 ttggccgcca gatcggccct atcgtccagc ctcaccagca gcatcgccgt tcccgcgccc
     2101 tgcccagaga atgccgcgcg catgga
//
LOCUS       DATALS010000421         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_293269, whole genome shotgun sequence.
ACCESSION   DATALS010000421 DATALS010000000
VERSION     DATALS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293269"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1700
                     /locus_tag="VHS06_09360"
     CDS             <1..1700
                     /locus_tag="VHS06_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2988365.1"
                     /translation="ENRIVMASGPLGGTSIMGTGNVSFVMKGAMTNGATTTQANGFMG
                     AYMKFAGFDAVIVQGQSPKWVYIYMHDGTAELRDAEHLLGLDSYEIQDTITEELGKTE
                     RQLSVLGIGPAGEHQVRFAAISGDKGHVASHNGSGAVMGSKKLKAFVAERGKTRFPIA
                     DDKALKARANMIIEDILANPVAKGAYENGTMNSLAGMVKTGVLPVKNYTTNLYEGAEK
                     LQRSAYSQSWEMKPLPCWACRTKHLHMVTIKGGEFDGYTAEEPEYEQWAAWGSQVGNN
                     DAASAMVMAVEVDKYGMDTNETGWVMGWAIECFEKGIITREDTGGIELTWGNVPAIRE
                     MLRKIAMREGFGDLLAEGVKRASEKLGRGSQDMAIYTGKGNSPRGHDHRSRWIEMVDT
                     CISDTGTMAVGPAWLPQEQGAKANPDMYYWEDLAEQLGKHNGRMMFEDSLGICRFTSR
                     TSMESLAKAVEASTGWEDFTGEEAFAAGRRVSHLLRVFNLRCGITPDLEKPSKRYGSV
                     PVDGPMQGKDHLYVHWEDIRQRYYELMGWDLKTGRPFPETLKGAGLADLIPDAWPNQ"
     gene            1804..2664
                     /locus_tag="VHS06_09365"
     CDS             1804..2664
                     /locus_tag="VHS06_09365"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2988366.1"
                     /translation="MKLGFVGLGNMGCPMALNLIRAGHDLTVFDLSAGAVQRLVAAGA
                     IAADSAASVAEAGEIVLSSLPNPAAVEAVYLGQGGLIEGARPGQLFVDLSSISPSVAK
                     KVASAFAAKDAAMMDAPVSGGVKGATEATLAIMVGGSADAFERAVPVLNCIGKNVFHV
                     GPVGTSSTIKILNQLLVGINNAAVTEMIVLGRAAGMDLAQVKEVIDASSGWSRILETE
                     FPKAMNRDFDTGFAVDLMAKDLRLARDLGKELGVELPAVHMAVSHFERAGAAGLGKKD
                     VSAAIQLYEK"
BASE COUNT          536 a          762 c          952 g          439 t
ORIGIN      
        1 cggagaacag gattgtgatg gcttccggcc ctctcggcgg cacctccatc atggggacgg
       61 ggaacgtctc tttcgtgatg aagggggcca tgaccaacgg cgctacgacg actcaggcca
      121 acggctttat gggtgcctac atgaagtttg ccgggttcga tgcggtgatc gtgcaggggc
      181 agtccccaaa gtgggtgtac atctacatgc acgacggcac ggccgaactg cgggatgccg
      241 agcatcttct tggactggat agctacgaga tccaggacac cattacggag gaactgggca
      301 agacggagcg ccagctaagc gtgctgggga tcggcccggc gggcgagcat caggtgcgct
      361 ttgcggccat atccggggac aagggccacg tggccagcca caacggttcc ggcgccgtga
      421 tgggttccaa gaagctgaag gcgtttgtgg ccgagcgcgg caagacgcgc ttccccattg
      481 ccgacgataa ggcgctgaag gcgcgggcca acatgatcat cgaggacatc ttggccaacc
      541 ctgtcgccaa gggcgcgtac gagaacggga cgatgaactc ccttgccgga atggtgaaga
      601 ccggcgtgtt gccggtgaag aactacacga ccaatctgta cgagggcgcc gagaaactcc
      661 agcgctcagc ctactcccag agctgggaga tgaagccgtt gccatgctgg gcgtgccgaa
      721 caaagcacct ccacatggtg accatcaagg gcggcgagtt cgatggctac acggccgagg
      781 agccggagta cgagcagtgg gcggcctggg gtagccaggt aggcaacaac gacgccgcgt
      841 cggcgatggt gatggccgtc gaggtcgaca agtacggcat ggacaccaac gagaccggct
      901 gggtcatggg ctgggcgatc gagtgcttcg agaaggggat catcaccagg gaagacaccg
      961 gcgggatcga gttgacgtgg ggcaacgtcc cagcgatcag ggagatgctt cggaagatcg
     1021 ccatgcgtga ggggtttggc gatctgctgg ccgagggcgt caagagggca tccgagaagt
     1081 tgggtcgcgg cagccaggac atggcgatct acaccgggaa gggcaacagc ccgcgaggcc
     1141 acgaccaccg aagccgatgg atcgagatgg tggacacctg catctccgac accggcacca
     1201 tggccgtagg tccggcgtgg ctgcctcagg agcagggggc gaaggccaac cctgacatgt
     1261 actactggga ggatctggcc gagcagttgg gcaagcacaa cggccgcatg atgttcgagg
     1321 acagcctggg gatctgccgc ttcaccagcc ggacctccat ggagagcttg gccaaggcgg
     1381 tggaggcgtc caccggctgg gaggacttta ccggcgaaga ggctttcgcg gccggcaggc
     1441 gggtgagcca tctgctgcgg gtgttcaacc tgcgctgtgg catcacgccc gatctggaga
     1501 agccctccaa gcggtacggc tccgttccgg tggacggccc gatgcagggt aaggatcacc
     1561 tgtacgtgca ctgggaggac atccggcagc ggtactacga gctgatgggg tgggatctca
     1621 agacgggtcg accgttcccc gagaccctca agggggccgg tctggccgac ctcatcccgg
     1681 atgcatggcc caatcaatag cgttcgctgc agcacagcac taggcgttca caaggcatcc
     1741 aagcggctgc cggccacgtc ggccgtcagc cgcttggcat atatcggcga ggagagagcg
     1801 aggatgaagc tgggctttgt gggtctcggc aacatgggtt gtcctatggc gctcaacctg
     1861 atcagggcgg gccacgacct aaccgttttc gacctctcag ctggggcggt gcaacgactg
     1921 gtggcggccg gcgcgatcgc ggccgacagc gcggcgtcgg tcgccgaggc gggtgagata
     1981 gtcctatctt ccctgccaaa ccccgcggcc gtggaggcgg tctacctggg ccagggcggg
     2041 ctgatcgagg gcgcgcgccc cggacaactc ttcgtggatc tgagcagcat ctcaccctct
     2101 gtggcgaaga aggtggcgtc ggccttcgct gccaaggatg cggcgatgat ggacgcgccg
     2161 gtcagcggcg gggtgaaggg ggcaactgag gcgacgctcg ccatcatggt cggcggcagt
     2221 gcggatgcct tcgagcgtgc cgtgccggtc ttgaactgta tcggaaagaa cgtcttccac
     2281 gtcgggcctg taggtaccag cagcaccatc aagatcctga accagctgct ggtcggcatc
     2341 aacaacgctg ccgtgacgga gatgatcgtc ctcgggcgtg ccgcgggcat ggacctggcg
     2401 caggtgaaag aggtgatcga cgccagctcc gggtggagtc ggatcctgga aaccgagttc
     2461 cccaaggcga tgaaccgcga tttcgatacc ggtttcgcag tggacctgat ggccaaggat
     2521 ctgcgacttg cgcgggatct gggcaaggaa ctcggcgtgg agctccctgc ggtgcatatg
     2581 gcggtctccc acttcgagcg agccggcgcg gctggcctcg gcaagaagga cgtgagcgcc
     2641 gcgatacagc tgtacgagaa gtaggtaccg ccacaaggat atgaggatt
//
LOCUS       DATALS010000422         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_430365, whole genome shotgun sequence.
ACCESSION   DATALS010000422 DATALS010000000
VERSION     DATALS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430365"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VHS06_09370"
     CDS             <1..492
                     /locus_tag="VHS06_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988367.1"
                     /translation="SAYYRTSVLMNSNENVYEWSAQSANTACGSYAQFILQGPSTYSI
                     MMVSWDSDHAWLYLWPDSLSPVATRAEITIEPGVFHTYRIEADGPTNTIYVDGTPRLS
                     ADLQNAAGLTTQVQFGYSHIFLEVPTYSKWDYIRVTTPEPATLAMFLLAIFYPRRRGA
                     GPR"
     gene            complement(715..1332)
                     /locus_tag="VHS06_09375"
     CDS             complement(715..1332)
                     /locus_tag="VHS06_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988368.1"
                     /translation="MYWAEAGVGSNRIMRANLDGSGSERLLTTSSGYPCGLTLDLVND
                     QMYWLNLQTSTMYRSNLDGTGTVQVLKTGVSPVDLALDVAGGKVYWTENGASYPAYDV
                     IKRANLDGTGIETIVTGLWNPLGIALDLSARKVYWADHGQGKIQRANLDGTGIEDILT
                     GLGTPEFIALVPEPSCIILVIFGVLVFAGRRKQSPDNVGQCGTVT"
     gene            complement(1373..>2530)
                     /locus_tag="VHS06_09380"
     CDS             complement(1373..>2530)
                     /locus_tag="VHS06_09380"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2988369.1"
                     /translation="VEHLLAAEVGEALGRQRHERREDEGQIGYRNGYKARQLRTGEGR
                     IEVDVPQVRGLEGPYRSQIWQGLGTRSVNLEKLVAEMYVRGLSTRDIEDLLKEISDDG
                     QRPLLSKSTVSEVTEVLWEEYQAFTKRDLSAFDVVYLFVDAVYESLKQQANCRQAILV
                     SWGILSDGSKVLLHMSLGNKESQSSWLEHFRSLVSRGLPSPLTITSDGAPGLIAAVEA
                     MWPESERIRCWFHKMQNVLDKVPEAMREQIKKLLQDVRDAPDYETGKARAGKLIQEYQ
                     KPLPSAMACLADDLEASLAHLKLPSLHQKAIRTTNLAERGFVEERRRAKVIPKFRTEE
                     ECLKLVFGTLWRASERWRQVKFSALEKKQLEAYIRVRQAMGKKVREVLSAA"
BASE COUNT          470 a          877 c          654 g          529 t
ORIGIN      
        1 agtgcatact accgcacgtc cgttctaatg aactcgaacg agaacgtcta cgagtggtca
       61 gctcagtcag caaatactgc ctgtggctca tacgcgcagt tcattctcca ggggccatcg
      121 acctacagca tcatgatggt ctcatgggac tctgatcacg catggcttta tctctggcca
      181 gattcgcttt cgcctgtggc tacgcgggca gagataacca tagagcccgg tgtattccac
      241 acctaccgta tcgaagccga tgggccaacc aacacgattt acgtagacgg caccccgcgc
      301 ttatccgcag accttcagaa tgcggcgggc ctcacaaccc aagtccagtt cgggtactcg
      361 cacatcttcc ttgaggtccc gacatacagc aagtgggact acatccgcgt cacaactccc
      421 gagccagcta cgctggccat gttcctgctg gcaatcttct accctcgtcg tcgtggcgcc
      481 gggccgcgct gaaggaactt tgcgggtgtg agcctcttca cagcctcgtt gcgacgtgcc
      541 taagggttcc ccgacaggac caccagatcg acgtccgctc gtgatagcct actacctcgt
      601 cagtcgctgt catccaagac tcgtgaagga caatggatga agccagtagc gatgctagga
      661 atccaggaat ccgggacagt cacctattcc tcggagctga tcggcgtgga gaaactaggt
      721 gactgtccca cattgtccca cattgtccgg actctgcttc ctcctgccag cgaaaacgag
      781 caccccaaaa atgacaagta ttatgcagct aggctccggg acgagtgcaa tgaactcagg
      841 agtccctagg cccgtcaaga tgtcctcaat tccggtacca tcgagattgg ctcgttgaat
      901 cttgccttgg ccatgatctg cccagtaaac cttgcgcgcg ctcagatcca aggcgatgcc
      961 aagcgggttc cacaggcccg tcacaatcgt ctcgattcct gtgccgtcaa gattggctcg
     1021 cttgatcaca tcgtaggcag ggtatgacgc gccgttctcg gtccagtata ccttcccgcc
     1081 ggcgacatcg agggcaagat caaccgggct cacgcccgtc ttgagcacct gcaccgtgcc
     1141 agtcccgtcc agattggacc ggtacatggt gctggtctgc agattcagcc agtacatctg
     1201 gtcgttgacc aggtcgagcg tgagtccgca aggatagccg ctcgatgtgg tcagcaaccg
     1261 ttcactgccg cttccgtcca gattggcgcg catgatccga tttgagccca cgcccgcttc
     1321 ggcccagtac atcggtcaag tccagaatcg tctgtaaatt cagcggtccg gtttacgcgg
     1381 cggacaatac ctcgcgcacc ttctttccca tagcttgacg cacgcggata tacgcctcca
     1441 gttgcttctt ctccagcgcg ctgaacttta cctgccgcca ccgctcgctc gcccgccata
     1501 gcgttccgaa caccagcttc aggcactcct cctcggtgcg gaacttcggg atcaccttcg
     1561 ctcgccgccg ctcctccacg aagccccgct ccgccaggtt cgtcgtgcgg atcgccttct
     1621 ggtgcaggct cggcagcttc aggtgggcca gcgaagcctc caggtcatcc gccaggcaag
     1681 ccatggcgct gggcaacggc ttctggtact cctggatcag tttccccgcc ctcgccttgc
     1741 ccgtctcgta atccggggcg tcacgcacgt cctgcaagag cttcttgatc tgctcccgca
     1801 tggcctctgg aaccttgtcc agcacgttct gcatcttgtg aaaccagcag cggatccgct
     1861 cgctctccgg ccacatggct tcaaccgccg caatcaggcc cggcgccccg tcgctggtga
     1921 tcgtcagcgg tgaaggcaag ccccggctca ccagcgagcg gaagtgctcc agccaactgc
     1981 tctggctctc cttgttgcca aggctcatgt gcaacagcac cttcgagcca tcactcaaga
     2041 tcccccagct caccaggatc gcctgccggc agttggcctg ctgcttgagg ctctcgtaca
     2101 ccgcatccac gaacaggtag accacgtcga acgccgacag gtcccgcttg gtgaaggcct
     2161 gatactcctc ccacaggacc tccgtcacct cgctgaccgt gctcttgctc aagagcggcc
     2221 tctgcccgtc gtcgctgatc tctttgagca ggtcctcgat gtcgcgcgtc gagaggcccc
     2281 gcacatacat ctccgccacc agcttctcca ggttcacgct ccgcgttccc agcccctgcc
     2341 agatctgcga gcggtacggc ccttccagcc cacgcacctg cggcacgtcc acctcgatcc
     2401 ttccttcgcc ggtcctaagc tggcgggcct tgtagccgtt ccggtagccg atctggccct
     2461 cgtcctcgcg gcgttcatgc cgctgccgac ccaaggcctc gcccacctcc gccgccagca
     2521 gatgctcgac
//
LOCUS       DATALS010000423         4930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_67317,
            whole genome shotgun sequence.
ACCESSION   DATALS010000423 DATALS010000000
VERSION     DATALS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67317"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VHS06_09385"
     CDS             complement(<1..1128)
                     /locus_tag="VHS06_09385"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2988370.1"
                     /translation="MGDLAKGMVHYQRSLAAWRHTGSTVYAAHVQNCIGLVHYYTGEY
                     AKALPILEDALAEVRQGGYLRVEAFVLGSLGDVQRDSGNPQTALEYYETALDIAQRVE
                     EMPLVNCLLDAMANAHRLLGDYATAERLLRQAIEQAEENQSPFELATYTISAGVLAEE
                     QGDLERSGELLREALDQLSDSGARRDMARGRFHFARTLFALGHREKAAHELEICLELC
                     RQLGYDAFMVSEGQRAGDLLNWAQSKGIGGRRLQLVYERLRGPLPPAPSNQRPKIRVE
                     PTGPNRIEVYAFGAARVLVDSRLVTTGDWAVERTKELFFYLLEQQHSLRKEQIVDDVW
                     PDVDLGKSNSQFHSTMYRLRRALFPQCVTYRDGRYQLTLGNC"
     gene            1188..1772
                     /locus_tag="VHS06_09390"
     CDS             1188..1772
                     /locus_tag="VHS06_09390"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HEX2988371.1"
                     /translation="MTHRCEGQILVRSTMYRLRRALFPQCVTYRDGRYQLTLGNCLWY
                     DSSEFVQLLDEADKTDLAHDKRIQLYERAIALHQGQFLEEVYSEWVTPHREEMEMRFL
                     NALTRLAKLRALDGALQEATDLLRKAVAADPFQEEAHRGIIQYTALMGDRVAALKHYR
                     SYVELLRDELDATPAPNILELADRIAHGRSVPTP"
     gene            2203..2742
                     /locus_tag="VHS06_09395"
     CDS             2203..2742
                     /locus_tag="VHS06_09395"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HEX2988372.1"
                     /translation="MAALPLRLGWLALLALAAQIYVIYYPGDGVEAARGLHAILMMGS
                     YLALGIVVAFNSRVKGMSIIGLGLLLNFLVMAANGGFMPVSREAILTTGTRVSDGLPS
                     EGMRLPRSKDVLLSAENTRLWMLSDTIMAPRMPLARVYSIGDLIVGLGIIFLIQAAML
                     PGRHPEALEEGMQATQMFS"
     gene            2765..3034
                     /locus_tag="VHS06_09400"
     CDS             2765..3034
                     /locus_tag="VHS06_09400"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Franean1_4349 family RiPP"
                     /protein_id="HEX2988373.1"
                     /translation="MLHKNLEKLVGTAVIDGEFRAILLRSPNDAVKEFELTEEELEAV
                     NSAGTGSIEEFAACIHAWITRAPKPRHASSGHWILDGSLAERVAV"
     gene            complement(3202..4875)
                     /locus_tag="VHS06_09405"
     CDS             complement(3202..4875)
                     /locus_tag="VHS06_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2988374.1"
                     /translation="MSHHRICAPLDPELLPDIVGTTGYTAEVPHAQEICRTVRTVTPE
                     TKVVVGGHHVTMSPNEFDRGEVDYVVSGEGEVTFPHLLGELEKGGDLSTVPGIGYRQG
                     GRLVFTPARPPLKNLDATSLPRRDLVEKYRSDYFFRFWDDVYSVETTRGCPYQCSFCS
                     VWRFFGGKVRFKSPERVLSEIEGLPSTCEYFCVVDDNFLASMPRTRQIVRMMQERGVR
                     KKFWIQGRADAMVRDPEGIAMAAKAGLSTVLMGLEAYREEELGHFNKGKDASVAVNDR
                     AIDIMHDNGIDIWGAFLIDPSWEERDFEGLIEYVKRKRIQFLQFTILTPLPGTQLYEE
                     KKHLIKAGWDQFDFFHSVLPTKLPPERFYEQMANLYRETVMSFSQMKEMIRQGRIPRK
                     GLQRAKDMLAQVTDPSSYLDSVQSDLGERIQRQLVDAQAAMERLQVEVGSVQVELGAR
                     LQVQLEDIQGLLEGLQSDARALQADLSSKWEGQLTSLRTQVESLQQEVGLRLRSPLEG
                     LQSDVREGLRTRVDVLRQEVEVLRIEVASRIQQGLEPFQARQSNRLVAP"
BASE COUNT         1027 a         1503 c         1336 g         1064 t
ORIGIN      
        1 acagttcccg agggtgagtt gatacctccc gtcccggtag gtgacgcatt gagggaatag
       61 ggcccgacgc agccggtaca tggtagagtg aaattgactg ttgctctttc ccaaatccac
      121 atccggccaa acgtcatcga cgatctgctc tttacgaagg ctgtgttgct gctccaacag
      181 gtaaaagaag agttctttcg tcctctcaac tgcccagtca cctgttgtga ccagccggga
      241 gtcgacgagg actctggccg cgccgaaggc gtagacttcg atacggtttg gcccagttgg
      301 ctccactcgg atctttggcc gctggttcga tggagcaggt ggaagagggc cacgcaggcg
      361 ctcatagact agttgaagtc ttcttcctcc gatcccttta gactgagccc agttcagcag
      421 gtcgcctgcg cgctgcccct ccgacaccat gaaagcgtcg tatcccagtt gtcggcagag
      481 ttccagacag atctccagtt cgtgagccgc cttctctcga tgccccaggg cgaacagtgt
      541 gcgcgcaaag tggaatcgac ctctggccat atcccttcga gccccagaat cggagagctg
      601 gtccagggcc tccctcagca gctcgccaga acgttctaga tctccctgtt cttccgccag
      661 aacccctgct gagattgtgt aggtggcgag ctcgaaggga gattggttct cctcagcctg
      721 ctcgatcgcc tgccgcagga gccgctctgc tgtggcatag tcccctagca gccgatgcgc
      781 gtttgccatg gcatcgagca gacagttcac caagggcatc tcctcaactc gctgtgcgat
      841 gtccagggcg gtctcgtagt actcgagtgc cgtctgaggg tttccggaat cgcgctgcac
      901 atcgcccaga ctgccgagca cgaacgcctc aacacgcagg tatccgccct ggcgaacctc
      961 ggcaagggca tcctccagga tgggtagtgc tttcgcatat tcgccagtgt agtaatgcac
     1021 caaccctatg cagttctgca cgtgcgctgc gtagacggta cttcccgtat gtcgccaagc
     1081 ggccagggat cgctgatagt ggaccattcc cttcgcaagg tcgcccatcc ggacggcgag
     1141 tatcccaatc gactgacaga ccgtggccgc gccgggctcg tcgtcctgtg actcatagat
     1201 gtgaagggca gattctagtt cgctctacca tgtaccggct gcgtcgggcc ctattccctc
     1261 aatgcgtcac ctaccgggac gggaggtatc aactcaccct cgggaactgt ctctggtacg
     1321 acagcagcga gttcgtccaa ctactggatg aggccgacaa gacagatctg gcgcatgaca
     1381 aacgaattca gctgtacgag cgcgccatcg cgctccacca gggccagttt ctcgaagaag
     1441 tgtactcaga atgggtgact ccccatcgtg aagagatgga gatgcgcttc ctcaacgctc
     1501 ttacgcgcct tgccaaacta cgggcactgg atggagccct gcaggaagca accgacctgc
     1561 tgcgcaaggc ggttgcggct gatccgttcc aggaagaagc tcaccgcggc atcatccagt
     1621 acaccgctct catgggagac agggttgctg cgctcaagca ctataggagt tacgtcgaat
     1681 tactccgcga tgaactggat gcaacgcccg caccaaacat ccttgagcta gccgatcgga
     1741 ttgcccacgg acggtcagtc ccaacgccct gattagattt ttcgtctcct tacactccgt
     1801 tcttgcgagc gatttgtgag aatcgcgcgg taaagttccc cgcaacaggg acccacgaca
     1861 ccggtcccga caaaagaaag aaaggggaac tcagacaatg acaactttcc gcagcgtcta
     1921 tctgaaggtc atctacgcgg tgctcaccct cacggctcta gctatcgccg ccggtgctcc
     1981 cgaaggctgg cccatgccgt aggcgtccac ctcatccggc ttgttccgag acgaggtgat
     2041 cgtggcggac tcgtctcccg atgaaagtaa gaagacactt ccagtccgtc tcgatgattc
     2101 gcaattcgac ccaatacgca gcgagggctc aggttgattc tcgtttttgc agtgctcatt
     2161 ggcattggcg tagcgttgtt ccgaggaggc acactcgcac gattggcggc gctcccgctt
     2221 cgccttggat ggctggcgct actagcgctg gcagcccaga tctacgtgat ctactatccg
     2281 ggtgacgggg tcgaagcggc gcgcggcctg catgctatcc tgatgatggg atcctaccta
     2341 gccctcggaa tcgtggtggc attcaactcg cgggtgaagg ggatgtcgat catcgggctg
     2401 gggttgctgc tgaacttctt ggtcatggca gcgaacggcg ggtttatgcc ggtatcgagg
     2461 gaggcaatcc taaccacagg cacaagagtc tctgatgggc tgccttcgga agggatgcgg
     2521 ctcccgagat ccaaagatgt gctgctgtcc gccgaaaaca caaggctgtg gatgctgtcg
     2581 gacacgatta tggcgccaag aatgcctctg gccagagtct atagtattgg cgacctgata
     2641 gtcggactgg ggataatctt tctcattcag gctgccatgt tacctggtag gcatccagag
     2701 gcacttgagg agggaatgca agctacccaa atgttctctt gactcctatt gtgatggaga
     2761 cacaatgcta cacaaaaacc tcgagaagct cgtcggcact gccgtcatcg acggtgagtt
     2821 ccgagcgatc cttctccgat cgccgaacga tgcagtaaag gagttcgagc tcacggagga
     2881 ggagttggag gccgtcaatt cagccggaac tgggtctatc gaggagtttg ccgcctgcat
     2941 tcatgcttgg atcactagag ccccaaagcc gaggcatgca tcgtctgggc attggattct
     3001 ggatggatcc ctcgcagaac gcgtagcggt gtagcctacc atcgagacga cccaaccgat
     3061 ggacgagatc gaccggcgcg gcatgcgagt cggtcgatct cacggaatca gcggtcaacc
     3121 atcagtcggc tgccctccgc tgaaagttga ccgctgatgt cttctcaagg gtaatgatcg
     3181 tcctcagtgc ggaaagcgag gtcacggagc aacgaggcga ttgctctgcc tggcctggaa
     3241 cggctccagc ccctgctgga tacggctagc tacctctatc ctaagcacct cgacctcttg
     3301 ccgaagtaca tcgactctgg tcctcaaccc ttctctcaca tctgactgca gcccctccag
     3361 cggtgagcgc agccgcaacc ctacttcttg ttgcaagctc tctacctgag tacgcagcga
     3421 tgtcagttga ccttcccact tgctgctcag gtctgcctgg agcgccctcg catcgctctg
     3481 cagcccttct agcaggccct ggatatcctc cagttggacc tgcaaccgtg cgcccaactc
     3541 cacctggaca ctccccactt cgacctggag acgttccatc gcagcctggg catccaccaa
     3601 ctgtctctga atcctctccc ctagatcaga ctgaacacta tccaggtagg atgagggatc
     3661 ggtcacctgt gccagcatgt ccttcgcacg ctgcagtccc ttccttggta tccgaccctg
     3721 cctgatcatc tctttcatct gcgagaaact cataaccgtt tcccgataca ggttggccat
     3781 ctgctcatag aagcgctctg ggggaagctt cgtcggaaga acgctgtgga agaaatcaaa
     3841 ctgatcccac ccagccttga tcaggtgttt cttctcctcg tagagttggg tcccaggcaa
     3901 gggagtcaga atagtgaact ggaggaattg gatccgcttg cgcttcacgt attctataag
     3961 tccttcgaag tccctctcct cccaactcgg atcgatcagg aaggcacccc aaatgtctat
     4021 cccattgtca tgcatgatgt ctatggctcg atcattcacg gccaccgagg catccttgcc
     4081 cttattgaag tgccccaact cttcttctcg atacgcttct agtcccatca ggaccgtcga
     4141 aaggcccgct ttcgccgcca tggcgatccc ttccggatct ctcaccatcg catctgcgcg
     4201 cccctggatc cagaacttct tccggacacc gcgctcctgc atcatccgta cgatctgcct
     4261 ggtcctgggc atggatgcca ggaaattgtc atccaccaca cagaagtact cgcaggttga
     4321 gggtaatccc tcgatctcgg acagcacccg ttcaggagac ttaaatcgga ccttacctcc
     4381 gaagaagcgc cacaccgaac agaagctgca ctgataaggg caccctctcg tagtctccac
     4441 ggagtacaca tcatcccaga accggaagaa gtagtccgat cggtatttct ccaccaggtc
     4501 ccggcgcggc agcgaagtcg cgtcgagatt cttcaagggc ggcctcgcag gagtgaacac
     4561 cagtctgccc ccctgtcggt atccaatacc tggaactgtc gaaagatccc cacccttctc
     4621 cagttcgccc agcagatgcg ggaaggtcac ctctccctct ccagagacca cgtagtcgac
     4681 ctcaccccgg tcgaactcgt tggggctcat ggtcacatgg tggccaccta caaccacctt
     4741 ggtctccggg gtcactgtac gcacagtccg acagatctcc tgggcatggg gaacttccgc
     4801 tgtatagccc gtagttccaa ctatgtccgg aagaagttct ggatccaggg gcgcgcagat
     4861 gcgatggtga gagatccgga agggatcgcc atggcggcga aagcgggcct ttcgacggtc
     4921 ctgatgggac
//
LOCUS       DATALS010000424         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_293506, whole genome shotgun sequence.
ACCESSION   DATALS010000424 DATALS010000000
VERSION     DATALS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293506"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VHS06_09410"
     CDS             complement(<1..966)
                     /locus_tag="VHS06_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2988375.1"
                     /translation="MSSLVTGIGFIGSRLVGLLLDRGETVVGLDNLFSTDREAIRQLA
                     SRPGFTFLEGDVADEDAVRRAFDVGDHIDTVYHLAAQSSSNPAAAPMSYSERANLVGP
                     RVVLEQACASGTSTFIFGGSLQVYGRQVSGVIDESHPYGPILDISHLSKLYVEKLMEM
                     FAINRGLRCVSARLALVYGVSPVTKSDPLFMTAPNKFCRQVARGESMRVDASAFNPTS
                     MIHVDDAARGLMSLSAWPRPAYSAVNLLGETASVAETARLVQEIASHRGLVVRLDYPQ
                     GEARLQLLPSQRFAEVGLQHHGSDGVDQHRLGFDHLSHDGDPPQTL"
     gene            1205..1960
                     /locus_tag="VHS06_09415"
     CDS             1205..1960
                     /locus_tag="VHS06_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX2988376.1"
                     /translation="MRSYYILGMRVDDVTFDEALSMIDEYIQSGKSHRVVTPNPEFAM
                     AARGDPHFLKALNGSSLAIPDGIGLMLAARLAGQRFREHVRGTDLVHRAAALCARKGY
                     RMFLLGAAPGVAEEAAARLVEANPGLQIAGCFAGVSGPQGDDETVAAISRAGRVDLIL
                     VAYGAPAQEAWLARNQERLGIPCGIGVGGVFNFLSGRSKRAPAWVRRIELEWLYRLIT
                     EPWRWKRQLVLPVFALTVLWERVRGRRPVRDAF"
     gene            complement(2104..2526)
                     /locus_tag="VHS06_09420"
     CDS             complement(2104..2526)
                     /locus_tag="VHS06_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX2988377.1"
                     /translation="MQILVVGCGRIGTYLVEELLAKGHQVAVVEKDPSRCEQLASENR
                     SDALRIVHGDGNDPIVLREAGARDADALAAATGEDEDNLVIGLLGKREFGIRRVIGRV
                     NNPRNAWLYSAAFGFDAAVRPAETIVSLIEEQIESPTP"
     gene            complement(2604..>2820)
                     /locus_tag="VHS06_09425"
     CDS             complement(2604..>2820)
                     /locus_tag="VHS06_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX2988378.1"
                     /translation="EVELIQFHASSRMAGYSVADLTSPSQFSVVSIVRGGRAFLPTAG
                     TQLEEGDLVYVASEAGSLAKLEEIIRA"
BASE COUNT          495 a          862 c          943 g          520 t
ORIGIN      
        1 gagtgtctgc ggcggatctc catcatggct gagatgatcg aagccaaggc ggtgctggtc
       61 cacgccatca ctcccatggt gctggagacc cacctcagcg aaacgctgag aagggagaag
      121 ctggaggcga gcctccccct gtgggtagtc cagccgcacc accagtccgc ggtgggaggc
      181 gatctcctgt accagcctgg cagtctctgc caccgaggcg gtctcgccca gcaggttgac
      241 ggcggagtag gcgggacgtg gccatgcgga caggctcatc agccccctgg ccgcgtcgtc
      301 cacgtggatc atgctggtgg gattgaaggc tgaggcatcc acccgcatcg actcgccacg
      361 ggcaacctgc cggcagaact tgttaggcgc ggtcatgaag agcggatcgg acttggtaac
      421 ggggctcacg ccgtacacca gcgcgagacg cgctgacacg cagcgcagtc cacggttgat
      481 ggcgaacatc tccataagct tttctacgta cagcttggag aggtgcgaga tgtccaggat
      541 gggcccgtag gggtgcgact cgtcgatcac tccagagacc tgccggccgt acacctggag
      601 ggagccaccg aagatgaagg tcgacgttcc cgaggcgcag gcttgctcca ggacgacgcg
      661 ggggccaacc aggttggccc gttcggagta gctcattgga gcggcggccg ggttagagct
      721 ggattgcgcc gccaggtggt agacggtgtc gatgtggtcc cccacgtcga aggctcgccg
      781 gacggcatcc tcgtcggcca cgtcgccctc gaggaaggtg aacccggggc gcgacgcaag
      841 ctgccgaatc gcttcacggt cggtggagaa gaggttgtct agcccaacca cggtctcacc
      901 gcggtcgagc agcagtccta ccaatctgga accgatgaag ccaatgcctg tgacgaggga
      961 ggacaatggg cgctccagtg cccctgcagg gcagacaaga atgatagcat cagcggcccg
     1021 acccaacaac ccgaggcgct gacggcatcc gctaccctat aatggtatgg cagagtgcgg
     1081 cagagctgag ggaccgattc caacaggatg tacaggatga acaggatcct gtctatcctg
     1141 tttgaatcgg tcttgaagcc atggctagct caaccgcgga agtagctgtg accaggggag
     1201 gagcatgagg tcgtactaca tactggggat gagggtggac gacgtcacct tcgacgaggc
     1261 cctgtccatg atcgacgaat acatacagtc agggaagagc catcgggtgg tgacgcccaa
     1321 cccagagttt gccatggcgg cgcgaggcga tccccacttc ctgaaggcgc tcaacggctc
     1381 ctccctggcg atccccgatg gtatcggact gatgctggca gcgcgattgg ccggccagcg
     1441 gtttcgcgag cacgtgcggg ggacggatct ggtgcaccga gccgcggcgc tctgcgctcg
     1501 taagggatac cgcatgtttt tgctgggtgc cgcccccggt gtggcggagg aggccgcggc
     1561 cagactggtg gaggcgaacc ccggccttca gatcgcgggc tgttttgccg gggtcagtgg
     1621 cccgcagggt gatgacgaga ccgtggcagc gatatcacgg gccggccgag tagacctgat
     1681 cctggtggcc tacggcgccc cggcgcagga agcgtggctt gccagaaacc aggagaggct
     1741 gggaataccg tgcgggatcg gtgtcggtgg ggtctttaac ttcctttccg gacgcagcaa
     1801 gcgggcgccg gcctgggtgc ggcgaataga gttggagtgg ctgtaccggc tgatcaccga
     1861 gccgtggcgg tggaagcgac agttggtgtt gccggtcttt gcgctgaccg tgctgtggga
     1921 gcgggttcgc ggtcgccggc cggtgcgaga cgcgttctga gttctgggtg ctgagcgcgg
     1981 gtgtcgggta tcaggggtca ggtatcaggg gggcaggtga cgggggccgg gggtcaggga
     2041 atgggttgca cgcagagcct gacctggtag actgcccccc aacccccaac ccctatcccc
     2101 caatcagggg gtgggtgact cgatctgctc ctcgatcagc gacacgatgg tctccgccgg
     2161 ccttaccgcc gcatcgaagc cgaaggctgc ggagtagagc caggcgttgc gcgggttgtt
     2221 caccctgccg atcaccctgc ggatcccgaa ctctcgcttt cccagcagcc cgattaccag
     2281 gttgtcctcg tcctcacccg tggcggccgc gagtgcatct gcatctctgg ctcccgcttc
     2341 tcgcagcacg atggggtcgt tgccatctcc atggacgatc cgcagtgcgt cgctcctgtt
     2401 ttcgctggcc agttgctcgc agcggctggg gtctttctcg accaccgcta cctggtgccc
     2461 cttggccagc agttcctcga ccagataggt gccgatcctc ccgcagccca cgaccagtat
     2521 ctgcattgtc cccctccctc tcccccgggc gtgtgttttc cgctacgatc ggaggctcgt
     2581 gcccattctc atgccgttgc ctgctatgcg cggattatct cttccagctt ggccagggat
     2641 cccgcctcac tcgccacgta cactaggtcg ccctcttcca attgggtccc agccgtgggg
     2701 aggaatgccc tcccgcccct gactatggag accacgctga attgggatgg actcgtgaga
     2761 tctgccacgc tgtagcccgc cattcgactg gaggcatgga actggatcag ctccacctcg
//
LOCUS       DATALS010000425         9388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_293596, whole genome shotgun sequence.
ACCESSION   DATALS010000425 DATALS010000000
VERSION     DATALS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293596"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..845)
                     /locus_tag="VHS06_09430"
     CDS             complement(294..845)
                     /locus_tag="VHS06_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988379.1"
                     /translation="MVLRVSAVGLGIAVIMASRLQTGSALAAPPSELAAVQTSVATPT
                     ATLSSVAAGIPTIIVRAGPHPPLPPAATTTPDAEGAAAPATPARVVEVSSTRSAPRTP
                     APTPYVSFSTESTTDSVGLTAEVLPARSAPASLPEAGSLNDTSPTALLATAGALAVAL
                     GILLSKVRKSFKPAGTTRSTTLD"
     gene            complement(1079..1726)
                     /locus_tag="VHS06_09435"
     CDS             complement(1079..1726)
                     /locus_tag="VHS06_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX2988380.1"
                     /translation="MWRPNELGRGPWPMDRGFARALLYGAALLTLIMVAAMPVTAEAR
                     PLAESGATAVPAEAPANVVAMSSPGDSSALSSLLPASPEQHPAEANLLDLLNSARVER
                     GLAALQLEPRLMALARQRSADLVTRNYFSHTTPEGTTVFDAMDKESIPYLLAGENLGR
                     CNYPLAESAQVVHQAFLKSPDHAENELDPSFNKVGIGMAVAPNGFVYYTELFAQQ"
     gene            2128..3141
                     /gene="trpS"
                     /locus_tag="VHS06_09440"
     CDS             2128..3141
                     /gene="trpS"
                     /locus_tag="VHS06_09440"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX2988381.1"
                     /translation="MDKNRKGRILTGDRPTGKLHLGHYVGTLRNRVRLQDEYECFFLI
                     ADYHVLTTRLQDLKEIEENIRDVLLDNLSAGIDPERSTIFLQSLVPQIPELHLLFSML
                     TSVPRLQRVPTLKEQMRDLKLETASYGLLGYPVLQASDILVVKGDLVPVGKDQTSHIE
                     LTREIARRFNELFGPVFPEAQALIGDVPTLVGIDGTAKMSKSLGNAIYLSDDAKTVEK
                     KVMTMYTDPTRIHATDPGHVAGNPVFIYHDVFNPNKDEVNDLKKRYRAGKVGDVEVKK
                     KLAKALNEFLEPIRERRAYYEQNPHLLDEIIQSGSEKVRAVAAETIRQAREAMGIYYF
                     RKG"
     gene            3406..3834
                     /locus_tag="VHS06_09445"
     CDS             3406..3834
                     /locus_tag="VHS06_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX2988382.1"
                     /translation="MQITRRADYAVRTMMDVAGLGEGATALTHEVASRQQLPAAFLAK
                     IVLSLTQAGLLRSYRGSGGGIALSRPAKEITLLQVVEAVDGPIAVNSCVLWPEECCRG
                     GNCSVHGVWCEAQRLLVAHLKGVTLAALVDKERAGCLDSG"
     gene            3922..5457
                     /gene="rny"
                     /locus_tag="VHS06_09450"
     CDS             3922..5457
                     /gene="rny"
                     /locus_tag="VHS06_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX2988383.1"
                     /translation="MDSTILILLSGLVMLLVGALVGYFVRQYLVSADMKLAQTSAEKL
                     IAEANAKKNEMLLDAKDEALRIRQAAEAEHRERRSDLQRQERRLQQKDENLERRVESV
                     ERREKAITNKEKELEAVRSEIEEVKVQQFQELERISGLTREVAKAEILRIVEEEVKVD
                     VARRVRELENQAREEAERKARGIITMAVQRCATDQVAETTVSVVPLPNDEMKGRIIGR
                     EGRNIRALESATGVDLIIDDTPDAVTLSGFDPVRREIARLALGKLVTDGRIHPARIEE
                     IVHKTRLEVEAAIKEAGEEAALEANVHGLHPEIIKLLGRMRYRTSYGQNIRLHSIEVA
                     HIAQILASEIGADVNIARRAGLLHDLGKAVDHEFEGPHALIGADLAKRYGESPKVIHA
                     IAAHHADETEPQTLEAVIVQAADAISGARPGARRETLEHYIKRLEALEGVANSFEGVE
                     KSFAIQAGREVRIIVKPEEIDDLGAVRLARDIVKRIEETLQYPGQVKVTVVRETRAVD
                     YAK"
     gene            5533..6381
                     /locus_tag="VHS06_09455"
     CDS             5533..6381
                     /locus_tag="VHS06_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2988384.1"
                     /translation="MTEKNFKVDLHLHTTASDGALTPTELVREAKAHGLECIAITDHD
                     STNGIAEALEEGARQGVQVIPGIEMSTDIPRAEVHVLGYFLDYHEPNFQKILQQLRDG
                     RRDRAEKMVAKLATMGLNLPWERVLEVAGKGSVGRPHIAQVMVEKGYVATMVEAFTNY
                     IGRNAPAYVERYKLTPAEAVALIKEVKGLPVLAHPAEVITLQSLLPELMSAGLVGMEC
                     YYSEYSPETVDGLLAVADQHGLIPTGGTDFHWHDPSGHGPKHPGETWVPWESVRRLKA
                     KATRTP"
     gene            complement(6570..7595)
                     /locus_tag="VHS06_09460"
     CDS             complement(6570..7595)
                     /locus_tag="VHS06_09460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX2988385.1"
                     /translation="MSREISLALVFAVAWLAAFLLTFVARAAGYRAGMVDKPRPGEVQ
                     KRPFPRTGGYAIYFAFVGAVLLGAFLVPRSDQESWRLTGVLLGSVAILPLAITDDRRR
                     LSPLPQLLGQIVIASVPVAFGVVADSIATPFWGVIPLPAWLVVPFTLIWIVGMINTIN
                     LVDVMDGLAAGLATIAALVLFIRCVFDFGQYDIAILPLALAGCCLGFLPHNWNPAKII
                     MGSSGALLLGYSLAVMSIMGGAKVATALLVLGVPIADVAWVIVRRLASGRSPMKGGDQ
                     RHLPQRLLLAGMSTRQIVLTFYLLCAVFGIAAARFSSVQKIYAFVAVAAVMTAIMVLL
                     ARSGHKA"
     gene            complement(7602..8681)
                     /locus_tag="VHS06_09465"
     CDS             complement(7602..8681)
                     /locus_tag="VHS06_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX2988386.1"
                     /translation="MSDTLSLIPQEKLLGRPSAPLELLGAEKIAGRRIAVTGAAGSVG
                     GPLVRMLAALAPSELLLLDNHEHTLFRLKLELGREMKVSTRWALADIRDRRKLGLLFK
                     ASRPEIVFHLAAYKHVPLGEENPDQTVEVNLAGTRALIEAAAEVGTSRFIYPSSDKAV
                     KPPSVYGGTKRIAEALALAANDRFDMEVSISRFVNIVGTRGSVIETFYGQITEGKPLT
                     LTDPGMTRYWITMPEATRLLVQTACHPESGVILMPDPGPAINLAEMARHLYGMLAPSP
                     NVECPIQFIGPRPGERMHEILLADTETARPTPYPGIVQVVSSLPERPDYGQVSQAIDA
                     LLGMATAGETTTLKGHVLDLAHSLQ"
     gene            complement(8678..>9388)
                     /locus_tag="VHS06_09470"
     CDS             complement(8678..>9388)
                     /locus_tag="VHS06_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX2988387.1"
                     /translation="ECLRRISIMAEMIEAKAVLVHAITPMVLETHLSETLRREKLEAS
                     LPLVEHSMETAEECGVVPLLENIPPVARQRQAAFMVTPIGMSAEDLVFFAEAFPGLGA
                     TVDLSHAQLFLNGLRMDPATAQPQFVPLVRYLSTLEDSPSIDQYLERVGKYLFEVHVS
                     NARGLLEEGLPYGDGDLDIDALVPRLGRTARYIVTETIEPNADRGVRMREAQRRLQAA
                     LLTIPGDDACPAHQEDPI"
BASE COUNT         1894 a         2703 c         3061 g         1730 t
ORIGIN      
        1 aatggtgatg gccggtttcg ctactagcgc cccaagacct tctgcgacca cgtcgaggac
       61 ggtctgtctg accaatccct acggtcttgg atcacagcat ccatctggga cgctattcag
      121 ttttggatac cagactacct ctccaacgtc cgacagtgcg cggtgcgggc ctaccacaac
      181 accactcgaa cctgggcaag atagcttctg ctaccacttt agatcagcag gctataggaa
      241 tgtcaacaac attgatacag agatgataca gcctttccct gttgatcacg ctgctaatcg
      301 agggtggtcg atcgggtggt cccggccggc ttgaacgact tccggacctt ggaaagtagg
      361 attcccagcg ctaccgccag tgccccagca gtggcaagca aggccgtggg gctcgtatcg
      421 ttgagggatc cggcctcggg gagtgaggcg ggagcggatc tggccggaag gacctctgcc
      481 gtcagcccca cggagtcggt ggtggactcc gtgctaaagc taacgtaagg ggtgggggcg
      541 ggggtgcgcg gagcagaccg tgtcgaagac acctcaacca ccctggcggg ggtggctgga
      601 gccgctgctc cctcggcgtc cggtgtggtg gtcgctgccg gcgggagagg cggatgcggg
      661 ccggcccgca cgatgatggt ggggatgccc gcggcaaccg atgagagtgt ggccgtaggc
      721 gtggcgacag aggtttgcac ggcagccagt tccgacggcg gtgcggcaag ggccgatcct
      781 gtctgcagcc gactcgccat aatcacggcg attccgagcc caaccgcgct cacgcgcagg
      841 accaccgaat cactccgccg agtcaagtcc ctctcttcga tgccttcgtc tgtctgttcc
      901 acatcatcat caacctacca ataccaccga tggctgtcaa gcccccttca acttgcgggg
      961 ggccgccgtt ggtggcatcg agggcactcc gacgagccgc tccggcgcca taaggattcg
     1021 gtcagaggat cgttgcacaa gatgggggcc aggggccagg ggacggggga cgcgcttgct
     1081 actgctgggc gaacagctcg gtgtaataga cgaagccgtt gggcgcgacg gccatgccga
     1141 tgccaacctt gttgaatgac ggatccaact cgttctcggc gtggtcgggg ctcttcaaga
     1201 atgcctggtg gactacctgc gcgctctccg ccagcgggta gttgcacctc ccaaggttct
     1261 ctcccgccaa gagataggga atactctcct tatccatggc gtcgaaaacc gtggtgccct
     1321 ccggcgtcgt gtgggagaag tagtttcggg tgaccaggtc ggcgcttcgc tgcctggcca
     1381 gcgccatgag tctgggctcc aactggaggg cagccaggcc acgctcgacc ctggcgctgt
     1441 tgaggaggtc gagcagattg gcctccgctg gatgctgttc aggagatgcg gggagcaacg
     1501 aactcagagc ggatgagtcc ccgggggaac tcatggcaac cacgttcgcc ggcgcctcgg
     1561 ccggaaccgc cgtcgccccg gactcagcca gcgggcgagc ttcagcggtt accggcatcg
     1621 cggcaaccat gatcagcgtg agcaacgcag ccccatagag gagggcgcgg gcgaatcccc
     1681 tatccattgg ccacgggccc cgtccgagtt cgttgggacg ccacatcaaa tgctctccac
     1741 tgcaagacca cggccgctag attggagact gccgttccga ccacaactac tttatcggca
     1801 gagagggccg ataacttgag cctttcggga ccggctgtcc gatggccgtc ggcggtcgat
     1861 catcgagccg gactgccacc atggcatcgg agagccatgg ggcgattgta ggtacatcga
     1921 aatgggcgac aatagcccga agttcctatt gaagtgaggc tcagacgctg tctagcgggc
     1981 gatgtctacc cgcacgagcc gagagatttc ccgcgtgcga tactgccacg aacatgagtt
     2041 gagagtgtct gccggccgcg ccgggtggca atagctgaat ggtccttgta aaatctgtcg
     2101 ggataagccg ttgggagaga gatattgatg gataagaacc gcaagggacg aattctcacg
     2161 ggcgacaggc ccaccggaaa gcttcacctc gggcactacg tggggacgct tcggaaccgc
     2221 gttcgactcc aggatgaata cgagtgtttc ttcttgattg ccgactacca cgtgttgacg
     2281 actcggcttc aggacttgaa agagatcgag gagaacatcc gcgatgtgct gctcgacaac
     2341 ttgagcgcgg ggatagaccc tgagaggagc acaatcttcc tgcagtcgtt ggtgccgcag
     2401 atacccgagc tgcatctgct gttttcgatg ttgacgtcgg tgccaaggtt gcagcgtgtt
     2461 cccactctca aggaacaaat gcgagatctg aagctcgaga ctgcctccta cgggttgctg
     2521 ggataccctg tgctccaggc ttcagacatt ctggtggtta agggcgatct ggtgcctgtg
     2581 ggcaaggatc aaacgtccca catcgagctc accagggaga tagcccgccg cttcaacgaa
     2641 ctgttcgggc cggtcttccc tgaggcccag gcgctgatcg gcgacgttcc gacgctggtg
     2701 ggcatagatg gcacggcgaa gatgagcaag agcctgggga atgccatcta cctctccgac
     2761 gacgccaaga cggtggagaa gaaggtgatg acgatgtaca ccgatcccac ccggatccac
     2821 gccaccgatc caggccacgt cgccggcaac cccgtcttta tctaccacga tgtcttcaac
     2881 ccgaacaagg acgaggtgaa cgatctcaag aagcggtacc gggccggcaa ggtgggcgac
     2941 gtggaggtga agaagaagct ggcgaaggcg ttgaacgagt tcctggagcc gatccgcgag
     3001 cgccgcgcct actacgagca gaaccctcac ctgctggacg agattatcca gtcgggaagc
     3061 gagaaggtgc gggcggtggc cgcggagacg attcgccagg ctcgagaagc gatgggcatc
     3121 tactacttca ggaagggctg atgctcggcc gctgttcagg tggtacatac tgtcatgctg
     3181 agcgcagcga agcatctgct cgtgaaggga gagatccttc gctgcgactc aggatgacac
     3241 ccgccagagc cgccgtgctc cggggttaga ggctaagggg ccgcggcccc atctgccctg
     3301 gaaatcgcca gcttcctcac gcgactccgt ttgactgcca cgaccctaac ccgtattcta
     3361 tatttgggca atgttggtcc aattactcat gctatggaga tgctgttgca gattaccagg
     3421 cgagcagact acgccgtccg caccatgatg gacgtagccg gccttgggga aggcgcaacg
     3481 gctctcaccc acgaggttgc atcacggcag cagcttccgg ctgctttcct ggccaagatc
     3541 gtcctatcct tgacgcaggc cggtcttcta cgttcctaca gggggtcggg cggcgggatt
     3601 gcgttgtcca gacctgcgaa ggagatcacc ctgttgcagg tggtggaggc ggttgacggc
     3661 cccatcgcgg tgaacagctg tgtgttgtgg ccggaggagt gctgcagggg cgggaactgc
     3721 tcggtgcacg gggtttggtg tgaggcccag cggctcctgg tagcacacct gaagggtgtc
     3781 acgctggctg ccttggtcga taaggaaagg gccggctgcc ttgacagtgg ttaagaaagt
     3841 ggatattatc atatagtggt catctgtacc tggcgcaacg atcctcctgt cagatagaga
     3901 aagagaggta gagcgctttt agtggacagt accatattaa tactgctaag tggcctcgtg
     3961 atgttgcttg tgggcgcatt ggtgggctac ttcgtccggc agtacttggt gagcgcggac
     4021 atgaagctgg cgcagactag cgccgagaag ctgattgccg aggccaacgc caagaagaac
     4081 gagatgcttc tcgacgctaa agacgaagct ctcaggatcc ggcaggcggc agaggctgag
     4141 catcgagaga ggcgaagcga tctgcagcgg caggagaggc ggctccagca gaaggatgag
     4201 aacctggagc gccgagttga gagtgtcgag cggcgggaga aggccattac caacaaagag
     4261 aaagaactgg aggcggtgcg ctccgagatc gaggaagtca aggtccagca gttccaggaa
     4321 ttggagcgga tctctgggct gactcgggag gtggccaagg ccgagatcct tcgcatcgtg
     4381 gaggaggagg tcaaggttga cgtcgcgcga cgcgtccgcg agcttgaaaa tcaggctcga
     4441 gaggaagcgg agcggaaggc caggggcatt atcaccatgg ccgttcagcg atgcgccacc
     4501 gatcaggtcg cagagacgac ggtatccgtc gttccccttc ccaacgatga gatgaaaggt
     4561 cggatcatcg gacgggaggg ccgtaacatt cgggcgctgg agagtgcgac cggggtcgat
     4621 ttgataatcg acgatacacc ggacgcggtt accctgtctg gtttcgatcc ggttcgcaga
     4681 gagatcgctc gtttggccct cggcaaactg gttacggacg gacgcattca tcctgcgagg
     4741 atcgaagaga tcgtccataa gaccaggctg gaggttgagg cggcgatcaa ggaggctggt
     4801 gaggaagcgg ctctggaggc aaacgttcac ggtttgcatc cggagatcat caagctgctt
     4861 ggccggatga ggtaccgcac cagctatggg cagaatattc gcctgcactc catcgaggtg
     4921 gcgcatattg cccagatcct cgcctcggaa atcggcgccg atgtcaacat agctcgaagg
     4981 gcaggtttgc tgcacgatct gggcaaggcc gtggaccacg agttcgaggg gccccacgca
     5041 ctgattggag cggatctggc caagcggtat ggcgagtcgc caaaggtgat ccatgccatt
     5101 gcggcacacc atgccgacga gacggagcct caaaccttgg aggcggtcat tgtccaggct
     5161 gccgacgcca tctctggtgc caggcctgga gctcgaaggg agaccttgga gcactacatc
     5221 aagaggctcg aggccctgga aggtgttgcc aattcctttg aaggtgtgga gaagtcgttc
     5281 gctatccagg ctggacgtga agtacgcatt atcgttaagc ccgaggagat cgacgatctg
     5341 ggtgccgtga ggttggctcg cgacattgtc aagaggatcg aggagacgtt gcagtatccg
     5401 ggtcaggtca aggtgaccgt ggtccgggaa accagggcgg ttgactacgc caagtaatat
     5461 cggataagtt gaggccggat gcgtcacgca tccggccttg atttttctct gcgggcaacg
     5521 gggagatatc cattgactga gaagaatttc aaggtagacc tccacctcca cactacagcc
     5581 tccgacggtg cgctgactcc gaccgaattg gtgcgggagg ccaaggcaca cggattggag
     5641 tgcattgcca tcaccgatca cgattccaca aatgggatcg cagaagcgct ggaggagggc
     5701 gcgcggcagg gcgtgcaggt gatacccggc atcgagatga gcacggacat tccccgcgcc
     5761 gaggttcacg tcctgggtta ctttctggac taccacgagc ccaacttcca gaagatcctg
     5821 caacagttgc gggatggccg ccgcgatagg gcggagaaga tggtggcaaa actcgcgacc
     5881 atggggctca atctcccgtg ggagagggtg ctggaggtcg ccggaaaggg ttccgtaggc
     5941 aggccgcaca ttgcacaggt gatggtggaa aagggctacg ttgcgaccat ggtggaagct
     6001 ttcaccaact atatcgggcg gaacgcgccg gcctacgtgg agcggtacaa gctgacaccg
     6061 gcagaggcag tcgccctcat caaggaggtg aagggactgc ctgttcttgc gcaccctgcc
     6121 gaggtgatca cacttcagtc gttgcttccg gagttgatgt ccgcggggtt ggtggggatg
     6181 gagtgctact actctgagta cagcccggag acggtggatg ggctgctggc tgtggcggac
     6241 cagcacggac tgatcccgac gggcggaacc gattttcact ggcacgatcc cagcggacat
     6301 gggcctaagc atccggggga aacctgggtg ccgtgggaat cggtgcgtcg tctgaaggcc
     6361 aaggccacgc ggactcctta atgctcttca atgctcttct ggagtgcagc ggcttccgcg
     6421 aggagactgc caagctcacc atcttggagt gcggcggctt gacgccgcaa ggtgggacgg
     6481 ggcagcgtta aggacggagt caagcgtgat ccccgagagc agggtgtgct gggctgcatt
     6541 gcggcgtcaa gccgccgcac tccagagcgc tacgccttgt ggccggagcg ggcgagtagc
     6601 accatgatcg ccgtcattac cgcggccacg gccacgaagg cgtagatctt ctgcaccgaa
     6661 ctgaaccggg cggcagcgat cccgaagacc gcacagagga gatagaaggt cagcactatc
     6721 tgccgcgtgg acattcctgc cagcaacagc cgttgcggca ggtgccgctg atccccaccc
     6781 ttcatcggcg atctgccgga tgcgagccgt ctcacgatca cccaggccac gtccgcgatg
     6841 gggaccccca gcaccagcag cgccgtagcc acctttgcgc cgcccattat ggacattacg
     6901 gccagactgt accccagaag cagcgctcca gagctcccca tgatgatctt cgcaggattc
     6961 cagttgtgcg gaaggaagcc caggcagcag ccggccagtg ccagagggag gatggcgata
     7021 tcgtactgac cgaagtcgaa cacgcatcgg atgaacagca ccagggcggc gatggtggct
     7081 aaccccgcgg ccagcccatc catcacgtcc accaggttga tggtgttgat catccccact
     7141 atccagatca gtgtgaacgg cactaccagc cacgccggca aagggatgac tccccagaag
     7201 ggggtggcga tgctgtccgc cacgacgccg aaggctaccg gcaccgaggc gatgacgatc
     7261 tgccccagta gctgcggaag gggtgagagg cggcgtcggt cgtccgtgat cgccagtggc
     7321 aggatggcca cggagcctaa tagcacgccg gtgagcctcc agctttcctg gtcggagcgg
     7381 gggaccagga aagcacccag cagaaccgcg ccgacgaagg caaagtagat tgcgtatccg
     7441 cccgtgcgag ggaaggggcg cttctgtacc tctcctggcc ggggcttatc taccatgccg
     7501 gccctgtaac ccgcggccct ggccacgaag gtgagcaaga aggccgcgag ccaggcaacc
     7561 gcgaagacca gtgcgaggga gatctctcta gacataagct cctactgtag cgagtgggcc
     7621 aggtccagca cgtggccctt caaggttgtg gtttctcccg ccgttgccat ccccaggagt
     7681 gcgtcgatgg cctgggagac ttgcccgtaa tccggccgct ccggcagcga cgagaccacc
     7741 tgcacgatcc cggggtaggg tgtcggccgc gccgtttctg tgtctgccag cagtatctcg
     7801 tgcatcctct cccccggcct cgggccgatg aattgaatcg ggcactctac gttgggcgag
     7861 ggcgccagca tcccgtacag gtggcgcgcc atttcggcca ggttgattgc cggtccaggg
     7921 tcgggcatca ggatgacgcc gctctcggga tggcaggcgg tctgaaccag gaggcgcgtg
     7981 gcctcgggca tcgttatcca gtagcgtgtc attcccggat cggtcagggt gaggggcttt
     8041 ccctcggtga tctggccgta gaaggtctcg atgacgctac ccctggtgcc cacgatgttc
     8101 acgaatcggg atatggagac ttccatatcg aaccggtcgt tggccgccag ggcgagcgcc
     8161 tcggcaatcc gtttggtgcc gccataaacg ctgggcggct tgacagcctt gtcgctggaa
     8221 gggtagatga agcgactggt acccacctcg gctgccgctt cgatcaacgc acgagtgccg
     8281 gccaggttaa cctccaccgt ctggtcgggg ttttcctccc ccagcgggac atgcttgtag
     8341 gcggcgaggt gaaacacgat ctccggacgg ctggctttga agagcaagcc gagcttacgc
     8401 cggtcgcgga tatctgccag tgcccatcga gtggaaacct tcatctcccg gcccaactcc
     8461 agcttgaggc ggaagagggt gtgctcgtgg ttgtccagaa gcagcagctc ggatggtgcc
     8521 aatgctgcca gcatgcgcac caacggtcca cccacggaac cggcggcacc ggtaaccgct
     8581 atcctccggc cggcgatctt ttctgccccc agcaactcca gcggtgccga gggccttccc
     8641 agcagctttt cctggggaat gaggctcagt gtgtcgctca aatgggatcc tcctgatgtg
     8701 cggggcaggc gtcgtcgccc ggaatcgtca gcagcgcggc ctgcagccgc cgctgagcct
     8761 cccgcatcct gacccctcga tcggcattgg gctctatagt ctcagtcaca atgtagcgtg
     8821 cagttcgccc caggcgaggg acgagggcgt caatgtccag gtcgccgtct ccatagggga
     8881 ggccttcctc cagcagcccc ctggcgttgg atacgtggac ctcgaacagg tacttgccca
     8941 ctcgctccag gtattggtct atcgagggac tatcctcgag ggtggatagg tagcgcacca
     9001 gcggcacaaa ttgcggctga gcggtggccg gatccatccg caggccgttc agaaagagct
     9061 gggcgtggga caggtccacg gtggcaccca gtcccgggaa tgcctccgca aagaagacca
     9121 aatcctcggc gctcataccg atgggagtga ccatgaaggc tgcctggcgc tggcgcgcga
     9181 cgggggggat gttctccagc agcggcacca ccccgcactc ctctgccgtt tccatggagt
     9241 gctccaccag ggggaggctc gcctccagct tctcccttct cagcgtttcg ctgaggtggg
     9301 tctccagcac catgggagtg atggcgtgga ccagcaccgc cttggcttcg atcatctcag
     9361 ccatgatgga gatccgccgc agacactc
//
LOCUS       DATALS010000426         3537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_341880, whole genome shotgun sequence.
ACCESSION   DATALS010000426 DATALS010000000
VERSION     DATALS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341880"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VHS06_09475"
     CDS             <1..351
                     /locus_tag="VHS06_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX2988388.1"
                     /translation="TIGTMASFIKSGKIRVLGVMDKQPAAAMPEAKTLESQGIKVYTT
                     TSRGYSAPAGTPKEIIDTLSAAIKKAADSEEHRKKLEEISTTVAYLDPAGFAAYWDDV
                     DAQVAPLIDLAKQK"
     gene            complement(473..1570)
                     /locus_tag="VHS06_09480"
     CDS             complement(473..1570)
                     /locus_tag="VHS06_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX2988389.1"
                     /translation="MKRILVSLVAVLAVVSVVGCSQAAPAATPTKAAAPAAQATAAPA
                     AAAQPTTAPAAAAQPTAAAKVNWPEKGKTVTIIVPVAAGGGSDVSTRVLQPSLEKTLG
                     TNIEVINKAGANQQLGHTEFSKAKPDGYTLAMTNLPTTFTNYLDADKKAVYNRSTFVP
                     VAAPVLDPGIICVTKDSPYKTMKDLVEAAKKDPGKISIGATGIMTAPHLMLLQAEKIT
                     GAKFNIVQFDKGAADATNALLGGHINGQSGFIGDLFTQVKSGNIRALAVMDTERSPFL
                     KDIPTMKELGYDLVYYTARGYSIQKGTPQGVVDVLENAFKVASSDPDVKKKQDEMGLT
                     QKYIGSKEYGALWDKMEKDAKPLIDEIRSAK"
     gene            complement(2059..3000)
                     /locus_tag="VHS06_09485"
     CDS             complement(2059..3000)
                     /locus_tag="VHS06_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2988390.1"
                     /translation="MSSLKMPIEGIAPGLTKAAAAHPELVILDADLPNGGAPNFNIHE
                     QFPGRTFEMGISEQTMVMTAAGLAASGKIPFALGLANFVALRAAEQIRTFVAHTKLNV
                     KIVTTLGGLSGGKEGPTHIATEDIAHMRAVPDLVVLAPADAVAAEKAVLAAADWKGPV
                     YISLGRTPTPVIYDDSFVFRIGKAEMHRDGTDATIISTGRMLYCALQAADLLEKEGLH
                     VRVLDMQTVKPLDEEAVLAAARETGAIVTLEEQTIVGALGSAVAEYLSGTRPTPVVRI
                     GLNDEYSETGKPEELVAHHGLVPENVARAVQRARELK"
     gene            complement(2997..>3537)
                     /locus_tag="VHS06_09490"
     CDS             complement(2997..>3537)
                     /locus_tag="VHS06_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2988391.1"
                     /translation="RLQGHCDMLKTPGIELTTGSLGHGLPAGEGMALAARMDGRPTRV
                     HVIIGDGESDEGLLWEAALSVPKFNLDNLVCILDRNQFQSSGPTSRIMPSLEPVADKW
                     RAFGWHVIEIDGHDFNQILDALDKAETIKGKPTFILAHTVKGKGLSFTEANNRWHVGV
                     LTDEEYRQAIKELSAEAVR"
BASE COUNT          603 a         1099 c         1125 g          710 t
ORIGIN      
        1 accatcggca ccatggcctc ctttatcaag tctgggaaga tccgggtcct gggcgtcatg
       61 gacaaacagc ctgcagcggc gatgccggaa gccaagaccc tggaatccca ggggatcaag
      121 gtctacacca ccacctctcg cggctactcg gcgcctgccg gtaccccgaa ggagatcatc
      181 gataccctga gtgctgccat caagaaggcg gcggacagcg aggagcacag gaagaagttg
      241 gaggagatca gcaccaccgt ggcctacctg gatccggccg gcttcgccgc atactgggat
      301 gatgtggacg ctcaggtggc tcccctgatc gacctggcca aacagaagta ggttgccctg
      361 gaaggcactc ccgcgatcag ccgcctctcg atgcaatcga agtcgcatca agcaaagagc
      421 ccccgcacat tatgtgcggg ggctctcgtt tacgatctgc tcagatggat acttacttgg
      481 cggaccggat ctcgtcgatc agaggcttgg cgtccttctc catcttgtcc cagagcgcac
      541 cgtactcctt ggagccgatg tacttctggg tcaggcccat ttcgtcctgc ttcttcttga
      601 cgtcgggatc ggaggaggca accttgaagg cattttccag gacgtccaca acgccctgcg
      661 gagtaccctt ctggatcgag tagccgcgag cggtgtagta caccaggtcg tagcccaact
      721 ccttcatggt cgggatgtcc ttcaggaagg ggctgcgctc ggtatccatc acagccaggg
      781 ctcggatgtt gccggacttt acctgggtga acaggtcgcc gatgaagcca gactgaccat
      841 tgatgtggcc gcccagcagc gcgttggtcg cgtcggctgc gcccttgtcg aactgaacga
      901 tgttgaactt ggcgccggtg atcttctcgg cctggagcag catcaggtgg ggagccgtca
      961 tgatgccggt ggcgccgatg gagatcttac cgggatcctt cttggcagcc tcgaccaggt
     1021 ccttcatcgt cttgtagggg ctgtccttgg tcacgcagat gatgcccggg tccaggaccg
     1081 gggccgcgac cggaacgaag gtcgagcggt tgtagaccgc cttcttgtca gcatccaggt
     1141 agttcgtgaa ggtggtcggc aggttggtca tggccagcgt gtagccgtct ggcttcgcct
     1201 tggagaactc ggtgtggccc aactgctggt tggcgccggc cttgttgatc acctcgatgt
     1261 tggtgccgag ggtcttctcc agagaaggct gcaagacgcg ggtggatacg tcgctgccgc
     1321 cgcctgcggc cacgggcacg atgatggtga cggtcttgcc cttctcgggc cagttgacct
     1381 tagctgccgc ggtgggctga gccgcggctg ctggggccgt ggtgggctgg gctgcagccg
     1441 caggggccgc agtagcctgg gccgcgggcg cagccgcctt ggttggtgtc gccgcgggcg
     1501 ccgcctggga gcagcccacg acgcttacca ctgccaggac tgccaccaga gaaaccagta
     1561 tgcgtttcat cttctcctcc ttgagaattg tccggtggac gccgcaggcg atccgaagca
     1621 gacatctgcg gatgccttcc gcgcagggac ttcccgagcc tatcctacag gtcgaaacca
     1681 ggcgccgttc agcgtcgggg agcgccctct acagaggttg gaaccgctac cgtggggagg
     1741 tggcaacaca cgggttgggg tttggccttg ctgccacgat ccttcagcgt gatgtccgtc
     1801 aacaggtagc ctacgataac cagagcgcca ttttgtcaca aggtggaacg tggcgcaagc
     1861 ggatcagctg tagagtgtac ggcatatgcc aagaatccat cgagcaagcc gcagtacatt
     1921 tcgcctatct cccgcgacta tacttgtggg aggtggcaac gaaacccttg cccatggact
     1981 gtgggaggtc agtctggaga atgagtggag atgataggca gggcatcgac atgccctgcc
     2041 tatcatctcg gtagaaagct acttgagttc cctggcccgt tgcactgctc gggccacgtt
     2101 ctccggcacc agcccgtggt gcgccaccaa ctcctccggc ttgccggttt cggagtactc
     2161 gtcgttgagc cctatgcgga ctaccggcgt cggccgggtt ccgctcaagt actcggccac
     2221 cgcgctgccc agggcgccca cgatggtctg ctcctcgagg gtgacgattg cacccgtctc
     2281 cctcgccgcg gccagcaccg cctcttcgtc cagcggcttg accgtctgca tgtcgagcac
     2341 ccgaacgtgc agcccctctt tctccagcag gtccgcagcc tggagggcgc agtagagcat
     2401 ccgtcccgta gagattatgg tggcgtcggt gccgtcgcga tgcatttccg ccttgccgat
     2461 tcggaagacg aaggagtcat cgtagatcac aggtgtagga gtacgaccga ggctgatgta
     2521 caccggacct ttccagtcgg ctgccgccag cacagccttc tcggcggcga cggcgtcggc
     2581 aggggccagc acgaccaggt cgggcaccgc ccgcatgtgg gcgatatcct cggtggcgat
     2641 gtgggtgggc ccctccttgc cgccgctcaa cccacccagg gtggtgacga tcttcacgtt
     2701 cagcttcgtg tgggccacga aggttcggat ctgctcggcc gccctgagtg ccacgaaatt
     2761 tgccagcccg agggcgaagg ggatcttgcc actcgcggcc agcccagccg cagtcatgac
     2821 catcgtctgc tccgagatcc ccatctcgaa ggtgcggccg gggaactgct cgtggatgtt
     2881 gaagttgggg gccccgccgt tgggcaggtc ggcgtccagt atcaccagtt ccgggtgtgc
     2941 agctgccgcc ttggtcagtc cgggcgcaat cccctctatc ggcatcttga gactgctcat
     3001 cggaccgcct ccgcgctcag ttctttgatt gcctgccggt actcctcatc ggtgagaacg
     3061 ccgacgtgcc agcggttgtt cgcctcggtg aatgagagcc ccttcccctt aaccgtgtgt
     3121 gccaggatga aggtcggctt tcccttgatc gtttcggcct tgtccagcgc gtccaggatc
     3181 tggttgaagt cgtggccgtc gatctcgatg acgtgccagc cgaaggcgcg ccacttgtct
     3241 gccaccggct ccagggaggg cattatccgg ctggtggggc ctgaggactg gaactggttc
     3301 cggtccagga tgcacaccag gttatccaga ttgaacttgg gaacggacag cgccgcctcc
     3361 cacagaagcc cctcgtcgga ctccccgtca ccgatgatca cgtggacgcg agtggggcgg
     3421 ccgtccatcc gggcagccaa cgccatgccc tcgccggcgg gcagaccgtg ccccagggat
     3481 ccggtggtca actcgatccc cggggtcttc agcatgtcgc agtggccctg gagcctg
//
LOCUS       DATALS010000427         1973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_432630, whole genome shotgun sequence.
ACCESSION   DATALS010000427 DATALS010000000
VERSION     DATALS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432630"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VHS06_09495"
     CDS             complement(<1..837)
                     /locus_tag="VHS06_09495"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEX2988392.1"
                     /translation="MSSYEETVIQRLAQFGERNDAIRAVLLTSSLCDPDAPVDVLSDF
                     DVEFFFEDPTPFVESDDWIQTLGMGSILALWHWPNEWDHEPGNGRSWMRMVYFEDGTR
                     VDISLCYMADLRALAGLDRLPDHYDIGYEVLLDKDGVTGSIKPASHRAFILRPPSEEQ
                     FASRAETFWMESIHVAKFLWRDEIVAAKWMLDGLRGRNLCEVLEWSVAIRHCWDWRPG
                     KLGRGLPKVLDPDTRRELIESYAAGDIEDMWQSLLRTTALYRKTASEVAGALGFEYPC
                     DLD"
     gene            complement(834..>1973)
                     /gene="miaB"
                     /locus_tag="VHS06_09500"
     CDS             complement(834..>1973)
                     /gene="miaB"
                     /locus_tag="VHS06_09500"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX2988393.1"
                     /translation="EIKMTRPGMIIGVCGCMAQRTGDSIRKGRPYIDIVVGTGSVSRV
                     PDLIRQVVESRRGASALDLPEARTDELALPERVERVGEALKSFVPVMYGCDNYCSYCV
                     VPYVRGRERSRPVADIVAEVRELAQHGRREITLLGQNVNSYGATLAEQTDFADLLQAV
                     ANVPEIERIRFTTSHPKDISYKLITAMRDIPQVCEHIHLAVQSGDDEVLRRMNRKYTS
                     AHFRERVAALRSAVPDIAITTDFLVGFPCETEEQFASTMRLAEDVRFDAAFMFAYNTI
                     PNTAAEKLKGQLSKTAKNERLGRLIALQNRITCEINDSQVGRVFEVLVEGKSPRDSRR
                     LTGLTRQGKTMNFPGDQSLIGPWVQVRATEGHLYGFVGELAERTA"
BASE COUNT          375 a          654 c          556 g          388 t
ORIGIN      
        1 gtccagatcg cacgggtact cgaacccaag ggctccggcc acctcgcttg ccgtcttcct
       61 gtagagagcg gtcgtgcgaa gaagtgactg ccacatgtcc tcgatatctc ctgccgcgta
      121 ggactcgatc agctccctac gtgtgtcagg gtcgaggacc ttgggcagtc ctctgcccag
      181 cttccccggc cgccagtccc agcagtgcct gatagcaaca gaccactcca gcacttcgca
      241 gaggttacga cctcgcaacc cgtccagcat ccatttggca gccactatct cgtcccgcca
      301 gaggaacttc gccacatgaa tggactccat ccagaacgtc tctgcgcgag aggcaaactg
      361 ctcctccgag ggaggcctga gaataaacgc cctatggctt gcgggcttga tggagcctgt
      421 gaccccgtcc ttgtccagga ggacctcata gccgatatca tagtgatcag ggagccgatc
      481 caggcccgca agcgcgcgaa gatcagccat ataacagagg ctaatatcca ctctggtacc
      541 gtcctcgaag tagaccatcc gcatccaact gcggccattg cccggctcgt ggtcccattc
      601 attcggccaa tgccacaacg ccaggataga acccatcccc agcgtctgaa tccagtcatc
      661 gctctccacg aacggcgtcg gatcctcgaa gaagaactca acgtcgaagt cagacagcac
      721 atcgacaggc gcatccgggt cacagagcga gctggtcagg agcacggctc tgatggcgtc
      781 gttacgctcg ccgaactgag ccagtctctg aatgaccgtc tcctcgtagc tgctcacgct
      841 gtcctctccg ccagttctcc cacaaacccg tacaaatgcc cctcagtcgc cctcacctgc
      901 acccacggtc cgatcaggct ctggtcaccg gggaagttca tagtcttccc ctgccgtgtg
      961 agacccgtaa gcctgcggga atcccggggg ctcttgccct cgaccagcac ctcgaagaca
     1021 cggccgacct gggaatcgtt gatctcgcag gtgatgcggt tctgaagcgc tatcaggcgg
     1081 ccaaggcgct cattcttcgc ggtcttcgag agctggcctt tgagcttctc cgcggctgtg
     1141 ttcggtatgg tattgtaggc gaacataaag gccgcgtcaa accggacgtc ctcggccaga
     1201 cgcattgtac tggcgaactg ctcctcggtc tcgcacggga agccgaccag gaaatccgtg
     1261 gtgatggcga tgtccggcac cgcgcttcgg agggcggcta cacgctcgcg gaagtgcgct
     1321 gacgtgtact ttcgattcat ccgccgcagc acctcatcgt cgcccgactg gacggccagg
     1381 tggatgtgct cgcagacctg tgggatgtcg cgcatggcgg tgatcagctt gtatgaaata
     1441 tccttcgggt gcgaggtggt gaagcgtatg cgctctatct ccggcacgtt ggccaccgcc
     1501 tgcagtaggt cggcgaagtc ggtctgctcc gcgagagtcg cgccatagga gttcacgttc
     1561 tgtcccagca gcgtgatctc acggcgacca tgctgtgcga gctcccgcac ttccgccaca
     1621 atgtccgcta ctggccggct gcgctctctg cctcggacat agggcaccac gcagtaggag
     1681 cagtagttgt cgcagccgta catcaccggc acgaagctct tcagcgcctc gcctacccgc
     1741 tcaacgcgct caggaagcgc aagttcgtct gttcttgcct caggcaggtc cagcgctgac
     1801 gctcctcgcc ggctctcaac tacctggcgg atcagatcag ggacgcgcga gacgctgccg
     1861 gtgccgacga cgatgtcgat atacggccgg ccctttcgga tggagtcgcc cgtccgctgg
     1921 gccatgcacc cgcacacgcc gatgatcatg cccggtcggg tcatcttgat ctc
//
LOCUS       DATALS010000428         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_294701, whole genome shotgun sequence.
ACCESSION   DATALS010000428 DATALS010000000
VERSION     DATALS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294701"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VHS06_09505"
     CDS             complement(<1..1009)
                     /locus_tag="VHS06_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX2988394.1"
                     /translation="MKALLSGNEAIALGAYHAGVAVAAAYPGTPSTEILEAVAGYADV
                     HAEWSPNEKVAMEVALGASYAGVRAMASMKHVGLNVAADPFFAAAITGVKGGLVVVAA
                     DDPGMHSSQNEQDNRHYARFAKVPVLEPSDSQEAYEFARLAFAISEEFDTPVLLRPTT
                     RVSHSMSVVEYDEALVPKRKAPSFTRQPDKYVMIPANARRRHPLVEERISRLAEYAES
                     FPHNRVDLRDRSLGVISDGVAYQYAREAMPHASFLKLGMGYPLPARMIRRFAEQVGRL
                     IVVEELDPFIEELVKAMGIPIEGKRYFSPVGELSLERVAAGFEKLSAVSRQPSVSSHQ
                     SV"
     gene            complement(1068..2321)
                     /locus_tag="VHS06_09510"
     CDS             complement(1068..2321)
                     /locus_tag="VHS06_09510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2988395.1"
                     /translation="MIRRPVFFGWTIVGALGITTIVSYGTTSYAFGVLLAPVAEDQGW
                     SRAAISGAYALGILVAGVLGVPIGLLVDRFGGRLLMSAGSALGGMALIALSMAQGVRE
                     FDLLWGVGLGLASALTFYPVSFTIVANWFEYRRGAALAWLTTLGGLASPIFIPATAWL
                     VAQVGWRQSLFILGLAQLAVGLPLHALVVRRHPEDLGLRPDGRVEPGQAGLAFPATDP
                     RSKGVTAHQALRSMVFWSLTLAAGIEQLAAMVVFAHQIALMISRGFDPVFAAGIAGLI
                     GLASLPGRFLLNRLSDQYDTQLLLASVLATLGLGVVVITLADSAAWLYLYAVVYGIAF
                     GARSSLRASVMAGHFGRRAYGAITAVQGGVISVPAAFGPLAAGWLYDRLGNYELAFWL
                     TAAAFALAALLILLTPRPRLDSLAL"
     gene            complement(2371..>2926)
                     /locus_tag="VHS06_09515"
     CDS             complement(2371..>2926)
                     /locus_tag="VHS06_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HEX2988396.1"
                     /translation="GLGQRARSWSGLVETIQREGTVQIRDASPDDAAAIARIYNQGIE
                     DRVATLETELRSPEERAEWLASHGTRHPVLVAVDGAGNVLGWGSINRFNPRKAYDHVV
                     DFSLYVDREHRGRGIGDAILGALEGRARSLGYHKMVLAGFPTNIPGKRLYERHGFSLV
                     GIYHEQGILDGRWIDVIIMEKILS"
BASE COUNT          589 a          976 c          896 g          465 t
ORIGIN      
        1 gaactgactg atggctgctg actgatggct ggcggcttac ggctgacagc ttctcgaacc
       61 ccgccgctac tcgctccagc gaaagctctc ccacaggaga gaagtaccgc tttccctcaa
      121 tcgggatgcc catcgccttg accaactctt cgatgaacgg atccagttcc tccacgacga
      181 tcagccgccc tacctgctcc gcgaaccggc ggatcatccg ggccgggagc ggatatccca
      241 tccccagctt caagaaggaa gcatgaggca tcgcctcgcg ggcatactgg taagcgaccc
      301 cgtccgagat cacgccgagc gatcgatccc gaagatcgac ccggttgtgc ggaaagctct
      361 ccgcgtactc tgccagacgg gagattctct cctccaccag cggatggcgc cgccgggcgt
      421 tggcggggat catcacgtac ttgtccggct gccgcgtgaa ggatggagct tttcgcttgg
      481 ggacgagcgc ctcgtcgtac tccacaacgg acatcgagtg agatacgcgg gtcgtcggac
      541 ggagcagcac gggcgtatcg aactcctcgc tgatggcgaa ggcgaggcgg gcgaactcgt
      601 acgcctcctg gctgtccgac ggctccagca ccggcacctt cgcgaagcgc gcgtagtgcc
      661 tgttatcctg ctcgttctgc gagctgtgca tcccgggatc gtccgcggcg accaccacca
      721 gcccgccctt gacccctgtg atggcggcgg cgaagaacgg atcggcggcg acgttcagcc
      781 ccacgtgctt catggaagcc atcgcccgga ccccggcgta agaggcaccc agcgcaacct
      841 ccatggcgac cttctcgttt ggcgaccact ccgcgtgcac atccgcgtag ccggccacgg
      901 cctcgagaat ctccgtgctc ggagtgccag gataagccgc cgccacagct acacccgcgt
      961 gataggcgcc aagggcgatg gcttcattgc cagagaggag agctttcacg ggaatccttg
     1021 ttctggctaa gttcagagga atggacgcaa ttataggcag caacctgcta gagggcaagc
     1081 gaatcgaggc gcggccgggg tgtcaggaga atcagcaacg ccgcgagcgc aaatgcggct
     1141 gccgtgagcc agaaggccag ctcgtagttg ccgaggcgat cgtagagcca tcccgcggca
     1201 agcgggccga aagctgccgg cacggagatc acgcctccct gcaccgccgt gatcgcccca
     1261 taggcgcgcc ggccaaagtg gccagccatg actgaagcac gcagggacga gcgcgcacca
     1321 aaggcgatcc catagacgac ggcgtataga tacagccacg ccgccgagtc ggcaagggtg
     1381 atcacgacca cgccgaggcc tagagttgcc agcaccgatg ccagcagaag ttgcgtgtcg
     1441 tactggtcgc tcaggcgatt cagcagaaac ctacctggta ggctggccaa cccgatcaga
     1501 ccggcgatcc ccgcggcaaa cacaggatcg aacccgcgcg agatcatgag cgcgatctgg
     1561 tgcgcgaaga ccaccatcgc cgcgagttgc tcgatgcccg cggccagggt caggctccag
     1621 aacaccatgc tcctcagcgc ctgatgcgcc gtcacgccct tcgatcgcgg gtccgtcgcc
     1681 ggaaatgcca gaccggcctg tccaggttca acccggccgt ccggacggag cccgaggtct
     1741 tccggatggc ggcgcacgac tagagcatgg agaggcagtc cgacggcaag ttgggccagg
     1801 ccgagtatga agagggactg ccgccatccg acctgagcaa ccagccacgc cgtcgccggt
     1861 atgaagatcg gcgatgccag gccgcccagg gtcgtgagcc aggccagcgc cgccccccgg
     1921 cggtattcga accagttggc gactatggtg aacgagactg gatagaaggt gagagcgctg
     1981 gcgaggccga gcccgactcc ccatagcagg tcgaactccc ggaccccctg cgccatcgag
     2041 agggcaatta acgccatccc accaagggcg ctgccagcgc tcatgaggag gcgcccaccg
     2101 aaacgatcga ccaggagacc gatcgggact ccaagtactc cggctaccag gataccgagg
     2161 gcataggctc ccgagatggc ggccctcgac cacccctggt cctccgcgac gggcgcgagc
     2221 aggacgccaa aggcgtatga ggtcgtgccg taggaaacga tcgtcgtgat tcccagggcg
     2281 ccgacgatcg tccagccgaa gaagaccggc ctacgaatca taggtcacac agtgttaact
     2341 caggatcttc tccatacagg tcacacagtg ttaactcagg atcttctcca tgatgatgac
     2401 gtcgatccat cgcccgtcga gaattccctg ttcgtggtag attcctacca ggctgaaacc
     2461 gtggcgctcg tacagccgct tcccagggat attcgtcggg aagcccgcca gcaccatctt
     2521 gtgatagccc agcgacctgg cccgaccttc cagcgcaccc aggatggcgt cgccgatgcc
     2581 gcgaccccgg tgctctcgat cgacgtagag cgagaagtcg acgacgtgat cgtaggcctt
     2641 gcgagggttg aagcgattga tggatcccca tcccaatacg ttgccggcac cgtcgacggc
     2701 gaccaggacc gggtgccgtg tcccatgaga cgccagccac tccgcccgtt cctcagggct
     2761 acgcagttcc gtctcgagcg tggcgacgcg gtcctcgata ccctggttat agatgcgagc
     2821 gattgccgcc gcatcatctg gcgacgcgtc ccgaatctgc accgttccct cccgttggat
     2881 agtctccacc agcccagacc aggatcgcgc ccgctggccc agtccg
//
LOCUS       DATALS010000429         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_433082, whole genome shotgun sequence.
ACCESSION   DATALS010000429 DATALS010000000
VERSION     DATALS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433082"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..791
                     /gene="rdgB"
                     /locus_tag="VHS06_09520"
     CDS             198..791
                     /gene="rdgB"
                     /locus_tag="VHS06_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX2988397.1"
                     /translation="MKLLIATNNQDKVREFREILEGLPVEVVTPAELGLQLEVEETGT
                     TFAQNAALKARAFHKASGLAALADDSGLEVDALDGEPGVYSSRYAGLPNGEVKNRFML
                     QKLEGVPWEKRGARYVCEIAIVDEKGRIHRCRGTLRGKIALEPKGRGGFGYDPIVYIP
                     SKRKTVAEMSPEEKNAISHRGLAGRRARVILERLLQS"
     gene            complement(1024..1398)
                     /locus_tag="VHS06_09525"
     CDS             complement(1024..1398)
                     /locus_tag="VHS06_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988398.1"
                     /translation="MRCARCGSRFDTGDNYCRRCGVTLDGRGLPTVVTRSLLPVPWSA
                     ARGPLIRGVVALAVGTVAEIVRREVARRVVSTDPSQALALLTAGKPVEGRRGLFSRSK
                     TPKGDYEVVETFVQRRLSFRKK"
     gene            1589..2590
                     /locus_tag="VHS06_09530"
     CDS             1589..2590
                     /locus_tag="VHS06_09530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2988399.1"
                     /translation="MTERAQPALKVTVIATVLNEESNVVGLIESLAHQTRIPDEIIIS
                     DGGSTDDTVERIRQAADRYSLNVDVLQLAGCNISEGRNAAVGRTTGDIIAVTDAGVRL
                     KPTWLEQLVTPFEKRPGRVDVVSGFFEPDPTTTFEVAMGATVLPAREDINPALFLPSS
                     RSVAFTREAWRRAGGYPEWLDYCEDVVFDIALRRNGCVFAWAPEAVALFRPRSRLVDF
                     WKQYYRYARGDGKAGLWPRRHAIRYGSYLAGLALLVAGRRKRAGLLLLAIASFLHVRR
                     PYQRLTPWLDGMEPDEKLQAISWVPILRLVGDLAKMAGYPAGVLWRMRQGRSITRLV"
     gene            2742..3635
                     /locus_tag="VHS06_09535"
     CDS             2742..3635
                     /locus_tag="VHS06_09535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2988400.1"
                     /translation="MATTPILSIVIVNYNTRDLLRDCLRSVQNSEGIQDMELFVVDNA
                     SSDGSAAMISEEFPSVQLIENPTNAGYAHANNLAITRCRGRYILLLNPDTVLPPDALV
                     RMIAFMDSNPHAGMAGPKLVRQNGSLDLACRRSFPSPEVSLYRMLGLSKLFPRSRRFG
                     RYNMTYVDDDQPIEVDSVVGAFMMVRDLAVKQVGMLDDSFFMYGEDLDWAYRVKAAGW
                     KVLYNPEVVVLHVKGAASSHHARKATIEFYRAMHIFYNKHYARSTNFLLGWLIVGAIW
                     AKCAFSLAMNCLRPAETRRVS"
     gene            3635..>3814
                     /locus_tag="VHS06_09540"
     CDS             3635..>3814
                     /locus_tag="VHS06_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988401.1"
                     /translation="MKRLFIISFGLSLMLTDIVLLLGAFAIGYNLRLRMEPRAQITMP
                     ALEAYQGIALLIVVSV"
BASE COUNT          763 a         1171 c         1172 g          708 t
ORIGIN      
        1 tagataccac cggccccgag ttaggagccc acaaccacga ggtatattgc gatctgctag
       61 gcttctccga ggaggaatta gctcagatga aatcggatgg agtgatctag tcaggtaggg
      121 actgagcagg cctgcaacag acatacatag tgatcgtagg ggcgacgcat gcgtcgcccc
      181 taaccaacag agtgaagatg aagctactca tagctaccaa caatcaggac aaggtgcggg
      241 agtttcggga gatcctggag ggtctgccgg tggaggttgt gacgccggcc gaattgggcc
      301 tgcagctgga ggtagaggaa accgggacaa ccttcgcaca gaacgcggca ctcaaggcgc
      361 gggcattcca caaggcctcg gggcttgcgg cgctggcaga cgactcaggt ctagaggttg
      421 atgccctgga tggcgagccg ggggtctact cgtctcgcta cgccggtctc cccaacgggg
      481 aagtgaagaa ccgtttcatg ctgcagaagt tggaaggggt gccctgggag aagcggggcg
      541 cccgctacgt gtgcgaaatc gccatcgtgg acgagaaggg gcggatacac cgttgccggg
      601 gaaccctgag gggcaagatc gcgttggagc caaagggccg gggaggattc ggctacgatc
      661 ccatcgtcta catcccatcg aagcgtaaga cggtagcgga gatgagtccc gaggagaaaa
      721 acgccatcag ccaccggggg ctggctgggc gccgagcgag ggtcatcctg gagagattgc
      781 tccaatcgtg agcgacagcc gggatagtac cggctggagg taaacggtca cggtggtcat
      841 atcgtagggc ggggtcttgt gcctcgcctc ccacacagtg gatgcgacca ccattctaat
      901 tcgggccacg ctctaggcgg ggcacaagac cccgccctac gtccaggcat cgcggccgct
      961 ctccgtgagt gcgagcatag ctcatgggtg acaagagcgt gcgtggtgct gccgttgcga
     1021 gccctacttt ttgcggaagg agagccgccg ctgtacgaag gtctcgacga cctcgtagtc
     1081 ccccttgggt gtcttcgacc tggagaacag gccacgcctg ccctccaccg gcttgcccgc
     1141 ggtgagcaga gccagcgcct gggatggatc cgtcgagacg acacgacgcg ccacctcccg
     1201 gcgaactatc tccgcaaccg tgccgaccgc cagcgcaacc acgccacgga tcaacggtcc
     1261 acgggcagca gaccagggta cggggagcag ggagcgggtg acgactgtgg ggaggccgcg
     1321 gccatcgagg gtaacgccgc aacggcggca gtagttgtca cctgtatcga agcgggaacc
     1381 gcaccgcgcg cagcgcatcg caacctcctc gcctctcgga ccggcacaga gtgatcaaca
     1441 accatccctt tctatcatac cgcgccgctt gacgcaacag gaacataaaa ctatcatcat
     1501 tctgccgtac cccggtcctc cagctgatgt gatgcagctt tcttgcagga gggccgcccc
     1561 cacccccatc tcgccgactg aggtatccat gacagaaagg gcgcaaccgg cactgaaggt
     1621 caccgtcatc gcgaccgtcc tcaacgaaga gtctaacgta gtggggctga tcgaatcgct
     1681 tgcccaccag acccgcatcc ccgacgagat catcatctcc gacggcggct ccaccgacga
     1741 cactgtggag cggattcgac aggcggctga ccgctactcg ctgaatgtgg acgtgctgca
     1801 actggcggga tgcaacatct ccgaggggcg caacgcggcc gtgggcagga caacaggtga
     1861 cattatcgcc gtgaccgacg caggggtgag gctgaaaccc acctggctgg agcagttggt
     1921 gacccctttc gagaaacggc ccggtcgagt tgacgtcgtc agtggcttct tcgagcccga
     1981 ccccaccacc actttcgagg tggcgatggg cgccacagtc cttcctgcca gggaggatat
     2041 taatcccgcc ctcttcctgc catccagtcg ctccgtggcc ttcaccagag aagcttggag
     2101 gcgagccgga ggatatcctg agtggctgga ttactgtgag gatgtagtgt tcgatattgc
     2161 actacgtcga aatggctgcg tcttcgcatg ggcaccggag gctgtagccc tcttccgccc
     2221 acgctcgaga ctggtcgact tctggaagca gtactaccgc tacgcgcgag gcgacggcaa
     2281 ggcggggctg tggccccgcc ggcacgccat ccgctacggc agttacctgg cagggcttgc
     2341 cctactggtg gccggccggc gaaagcgtgc cggtctgctg ctgctcgcca tcgcctcctt
     2401 cctgcacgtg aggcgtccgt accagcgact aacgccctgg ctggatggga tggagccgga
     2461 cgagaagctc caggccatct cctgggtgcc catcctccga ctcgtcggcg acctcgccaa
     2521 gatggccggt taccccgccg gcgtgctgtg gcgaatgcgg caggggcgct ccataactcg
     2581 cttagtttga cctcaaccag cttgctgcat ataatcgatc cggcttcaac tgccagagag
     2641 ctgttggtaa cctcggtctg gcgtcggtag ccgttcgttg gatgtatgcg tatccgaggg
     2701 tcatcgcccc atcgatcgcg cccatactat cgaggatagg cttggccaca accccgatac
     2761 tctccatcgt aatcgtcaac tacaataccc gcgacctact ccgcgattgc cttcgatcgg
     2821 tccagaacag cgagggaatc caggacatgg agctgttcgt ggtggacaac gcgtcatccg
     2881 acggcagcgc ggccatgatc tcggaagagt tcccatcggt ccaactgatc gagaacccca
     2941 ccaacgccgg ttacgcccat gccaacaacc tggccattac acgttgtcgg ggccgctaca
     3001 tcctcttgct caaccccgat acggtgttgc cgcccgacgc gctggttcgc atgatcgcat
     3061 tcatggactc caacccccat gcagggatgg cagggccgaa actggttcgc caaaacggca
     3121 gcctggatct ggcctgccgt cgcagtttcc ccagccccga ggtgtccctc taccgcatgc
     3181 tgggactcag caagctgttc cctaggagcc ggcgtttcgg tcgctacaac atgacctacg
     3241 tggacgacga ccagcccata gaggtggact ccgtggtggg tgccttcatg atggttcgag
     3301 accttgccgt taagcaggta gggatgctgg atgacagctt cttcatgtac ggcgaggacc
     3361 tggactgggc ctacagggtc aaggccgccg ggtggaaggt gctctataac cccgaggtgg
     3421 tggtgcttca cgtcaaaggt gccgccagca gccatcacgc acgcaaagcc accatcgagt
     3481 tctacagggc catgcatatc ttctacaata agcactacgc tcgcagtacc aatttcttgc
     3541 tgggctggct gattgtgggt gctatctggg ccaagtgtgc cttctccctg gctatgaact
     3601 gcttgcgccc cgcagagaca cggagggttt cctgatgaag cggctgttca tcatctcctt
     3661 cggcctctcc ctgatgctga cggatatagt gttgctgttg ggcgcgttcg ccatcggcta
     3721 caacctcagg ctccgcatgg agcccagggc gcagatcacg atgcccgcgc tcgaagcgta
     3781 ccaggggatt gccctcctca tagtcgtgag cgtc
//
LOCUS       DATALS010000430         3481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_68865,
            whole genome shotgun sequence.
ACCESSION   DATALS010000430 DATALS010000000
VERSION     DATALS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68865"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1850
                     /locus_tag="VHS06_09545"
     CDS             75..1850
                     /locus_tag="VHS06_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX2988402.1"
                     /translation="MSTIAANSNYGESPSGRPYTSRRIDRLVDALCLLGLGLSVLLLV
                     ASVGALGTFAYLRSTLALQPPIESPGENDTSVLEAAAPLLPCDATLGSALSVDFGSTG
                     PQPCATPEAQGSLLVAMPSDGTPTTVSGMGTFQALVAVLERGPSSPPGGARRTIITYK
                     VVAGDSLSSVAAAFGITPETVLWANDIKNPELLFAGQELKILPVSGVLHKVSSDDTVS
                     SVAESYGADPKAVREANGLTEGTEPKEGQELVVPGGILRTTELMEGPPAPLPAAEVQT
                     ATRYKVKAGDSLGSIADSFGVLPSTIQAANDLMDPDKLSIGQELAIPGARVTSAQGGQ
                     GEASTKPVAAPAQSVPPTAAPTSPPVPQAPVSAAASGAQYTVKSGDTLFSIAKAYGVK
                     PQSIQDANGLADPGKLKLGQTLLIPGVAANAQAAVQVATSTAVPQPPTATPVPPKPAV
                     AQPAPKPASQPAVPAGGSLGDRVAAIAQKYVGYRYIWGGHSPDGFDCSGFTWYVYREA
                     GYNIPLHDLNGQLNLGQKVTLDKLAPGDMVFFQNTYKQGLSHAGIYLGGGRFINAESE
                     KVGVQVRSISDPFWASRFVGASRPR"
     gene            complement(1855..2781)
                     /locus_tag="VHS06_09550"
     CDS             complement(1855..2781)
                     /locus_tag="VHS06_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988403.1"
                     /translation="MAFRLLWNSKGWDSGNVDPLLMAEDPLEPFRRTFEALGYSFDTI
                     FREDFAGQRAIRQASLGCEDEFRRRIVDSIARRGRPVIAIGVAGPPECCLVTGYDGDG
                     DTLIGWSYFQDMPEFAAGLRFEPAGYFRKRDWYANTFGLMSIGERSSRPSTSDLVRES
                     LSWALKLVRTPMVRDHYSGLAAYASWAETLSMDGEFPIHDSALLHQRFMVHNDAVSTV
                     AEGRWYAAQYLRRVAAQESSMAEQLLAAAACYEKEHDLMWELWEAVGGNGVSEDRVLR
                     LADPEVRRGMVSIILLASELDEEAAGQIERAL"
     gene            3252..>3481
                     /locus_tag="VHS06_09555"
     CDS             3252..>3481
                     /locus_tag="VHS06_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX2988404.1"
                     /translation="MRFDTSLVHQEGDNDPDTGAVSVPIYQASTFNQPDLENPTPFDY
                     SRSGNPTRAALEGAIASMEGGVAGFAFASGMAA"
BASE COUNT          624 a         1149 c         1110 g          598 t
ORIGIN      
        1 ccagtgtgtt cgcttcaaca ggggctattg ccgcggagca taactgccga tagtagatag
       61 tggagaggtg acaggtgtcc accatcgcgg ccaacagcaa ctacggggaa tccccatccg
      121 ggcgcccata cacgagccgg cgcatcgatc gtctggtgga tgcgctgtgc ctgctgggac
      181 tgggtttgtc tgtcctgctg ctggtcgcgt ccgtcggcgc cctgggtacc ttcgcctacc
      241 tgcgctctac actggcactc caacctccca tcgagagccc cggcgagaac gatacctccg
      301 tattggaagc agccgccccg ctgctccctt gcgacgctac gctcggctcc gccctgtctg
      361 tcgatttcgg ctcgaccggc ccgcaaccct gtgcgacgcc ggaagcgcag ggcagcttgc
      421 ttgtggcaat gccttcggac gggactccga ccacggtgtc tgggatgggc accttccagg
      481 cgctcgtcgc ggtcctggaa cgggggccct cgtcgccccc cgggggcgct cggcgcacga
      541 tcatcaccta caaagtcgtg gcaggggata gcctctccag cgtcgcggcg gccttcggga
      601 tcactcctga gacggtgctt tgggccaacg atatcaagaa ccccgagctg ttgttcgccg
      661 gccaggagtt gaagatactc ccggtatcgg gcgtgctgca taaggtatcc tccgacgaca
      721 cggtatcctc cgtcgcggag agctatggcg ccgatccaaa ggccgttcgg gaggcgaacg
      781 gcttgactga ggggaccgag ccgaaggagg gacaggagtt ggtggttcca ggtggcatcc
      841 tgaggaccac cgagttgatg gaggggccac cagctccgct tcccgcggct gaggtgcaga
      901 cggcgaccag gtacaaggtg aaggccggcg acagcctggg ttccatcgcc gacagtttcg
      961 gggtgctgcc gtctaccatc caggcagcca acgacctgat ggacccggat aagctgtcca
     1021 tcggccagga actggcgatc ccgggagcac gcgtcacatc cgcccagggt ggacagggtg
     1081 aagcctcgac aaagccggtc gccgcacctg cgcagagcgt ccccccgacg gcggctccga
     1141 cgtcgccccc agtgcctcag gccccggtat ccgccgcggc ctcgggcgcc caatacaccg
     1201 tcaagagcgg ggataccctc tttagcatcg ccaaggccta cggcgtgaag cctcaatcga
     1261 tccaggatgc caacggcctc gccgaccccg gaaagctgaa gctggggcaa acccttctga
     1321 tcccgggcgt cgcggccaac gcgcaggctg cggtgcaggt ggcaaccagc acggccgtcc
     1381 cgcagccccc cacagctacg cctgtgcctc ccaagcctgc tgttgcacag ccggccccca
     1441 agcccgcttc gcaaccagcg gttcccgccg gtggttccct tggggaccga gtcgcggcca
     1501 tcgcccagaa gtatgtgggg taccgctaca tatggggcgg gcactctccg gatggattcg
     1561 actgcagcgg ttttacctgg tacgtctacc gggaggcggg ctacaacatc cctctccacg
     1621 atctgaacgg gcagctgaac ctggggcaga aggtgactct ggacaagctg gcgcccggcg
     1681 atatggtgtt cttccagaac acctacaagc aggggttgtc ccacgccggc atctatctag
     1741 gtggtggcag gttcatcaac gcggagagcg agaaggtcgg ggttcaggtt cggtctatct
     1801 ccgacccctt ctgggccagc cgtttcgtcg gcgcatcccg gccgaggtag gtagctacag
     1861 cgccctctcg atttggccgg ctgcctcctc atccagctcg ctcgccagca ggatgatcga
     1921 caccattccc cgtcggacct ccggatctgc cagcctgagt acccgatcct cggagacccc
     1981 gttgcctccc acggcctccc acagctccca catcaggtcg tgctccttct cgtagcacgc
     2041 cgcggctgcc aggagttgct ccgccatgga gctttcctgg gctgcaactc tcctcaggta
     2101 ttgggcggcg taccaccgtc cctccgccac ggtactcacg gcgtcgttgt ggaccatgaa
     2161 acgctggtgg agcagggcac tgtcgtggat cgggaactcc ccgtccatgg atagggtttc
     2221 ggcccaggag gcataggctg cgagtccact gtagtggtcg cgcaccatcg gcgtgcgcac
     2281 cagcttcagt gcccaggaga ggctctccct cacaaggtcg ctggtcgagg gacgactgga
     2341 tctctcgcct atggacatga gcccgaaggt gtttgcgtac cagtcgcgct tgcggaagta
     2401 cccggccggc tcgaaccgca gcccagcggc gaactcaggc atatcctgga agtagctcca
     2461 tccgatgagg gtgtcgccgt caccgtcgta accggtaacg agacagcact cgggagggcc
     2521 cgcgacgcct atggcgatga ccgggcggcc cctgcgcgcg atggagtcga ctatccgacg
     2581 cctgaactcg tcctcgcagc cgagggaggc ctgtctgatc gcccgctgcc cggcgaagtc
     2641 ctctcgaaag atcgtgtcga agctgtaccc cagcgcctcg aaggtgcggc ggaatggttc
     2701 cagcgggtcc tcggccatca ggagcggatc gacgttgccg ctgtcccacc ccttggagtt
     2761 ccagagcagc cggaaagcca tccctgagat gcccgcgacg tacgcatagc tgcagccgag
     2821 gtgacgaacg gggtcgccag catggctgtg ctcgcagccc agctcgccgg cgtgctcgag
     2881 ccacgccctg aggcagccgg cgaaggggaa tccctcgggg gcagcgcccc ccgccgaggt
     2941 cgaaaccgat acgaggaacg ccttccagaa cggcgcggtg gggcacggca gtctgctccg
     3001 atccgaacgt catcaggaac ctcccatcca aggcatggcg ttggcggcaa gcggtggggt
     3061 ggcggaagga cccaagtgta gcaggtaagt tgatcataat ccaacatatg ggactgctcc
     3121 cgctgaaggt gaaagttgac tatgtcgata gaagaaccat acaattgtct gggctcggca
     3181 taccgcggcc gacacgaggc ggcgggccgg gcctacgttt cgaccgatca ctgccgcggg
     3241 agatgagata ggtgagattc gatacatcgt tggtccacca ggagggagac aacgacccgg
     3301 acacgggcgc cgtctccgtg cccatatacc aggcgtccac attcaaccag ccggacctgg
     3361 agaacccgac cccattcgac tacagccgct ccggaaatcc cactcgggcg gcgctggagg
     3421 gtgccattgc ctccatggag ggaggggttg ccggcttcgc ctttgcctcg gggatggcgg
     3481 c
//
LOCUS       DATALS010000431         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_296214, whole genome shotgun sequence.
ACCESSION   DATALS010000431 DATALS010000000
VERSION     DATALS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296214"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /gene="pncB"
                     /locus_tag="VHS06_09560"
     CDS             complement(<1..1542)
                     /gene="pncB"
                     /locus_tag="VHS06_09560"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019357 - nicotinate nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX2988405.1"
                     /translation="MKPTNPLVDPLLTDFYEYSMAYAWWLNGTHEEQAVVDVHFRKNP
                     FAGEFTVFAGVEEVLRFVSDYRFTDEQIEVVRRFMPADVDPAFFQWLKTVDCSKVRIR
                     ALREGSLAFPRVPLFIVEGPVAVVQLLEAPLLALVNYPSVVATGAARIRLAAGWDKTL
                     MEFGLRRAPGPDGGVSASRYSYIGGFDSTSNVKAAQLFDGLRAVGTHAHSWVQSFMGL
                     KDLKSRNIVGPDGREHDLVELVLRYREELGGNRTNEGELASFIAYAQAYPQGFLALVD
                     TYDTLRSGVPNFLSVALALREVGYRPMGIRIDSGDLSFLSKKARQLFREAGQRNGGDL
                     EKLIIVASNDIDEETLLALGQQGHEIDSFGIGTRLVTAGGETLGGVYKLTVAAGVPRI
                     KVSGDSAKITLPGKKRAFRLYAGDLPTPVVDLLTLADEAAPQPGERVLCRHPFDETKR
                     LIVIPSRVEDLYEVIWDGEPNTETVRPLNETRQYVLDQLAGFRRDHLRPVNPTPYKVS
                     VSAKLY"
     gene            complement(1662..>2961)
                     /locus_tag="VHS06_09565"
     CDS             complement(1662..>2961)
                     /locus_tag="VHS06_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEX2988406.1"
                     /translation="RTIGLGLQHVIAMFGATVLAPFLMGFDPNLAVFFSGIGTLIFML
                     VVRGRIPSYLGSSFAFIGPVIAAKANGGIPAALGGIAAAAVVYFIIGLLAKTDTGTKV
                     IATLMPPPVTAAVIAVIGIGLAPVAWGSASKDWTLALITLSVAIIVAVGTRGFTKLIP
                     ILIAVVVGYIVAAAMGKVDFSGVVAAPWIGLPNFVAPTFSLQAMSLIVPVVVMLVAEN
                     IGHVKAISAYMDRDLTPEVGNAFMGDAIATFISAMGGGTGQTTYAENIGVMSITKVYS
                     IRPLQVAAVTAILLGLVPKFGALIQSIPLPVMGGITLVLFGMIAGASTKVISSATEDW
                     GSIENFSVYGISIAVCAALVAVFNTNSALNDALKLATPLPTTLEIGFAQLDAIGSATF
                     FAVLLNLAIVVVKKLSPLAEGSAEAQPLATEDGREPATVQ"
BASE COUNT          565 a          893 c          933 g          570 t
ORIGIN      
        1 gtacagcttg gcgctgacgc tcaccttgta gggggtgggg ttgacggggc gcaggtgatc
       61 ccgccggaag ccggccaact ggtccaggac gtactgtcgg gtttcgttta ggggtcgcac
      121 cgtctccgtg tttggctcgc cgtcccagat tacctcgtac agatcctcta ctcgactggg
      181 aatgacgatc aacctcttcg tctcgtcgaa ggggtggcga cagagaaccc gctcgccagg
      241 ctgcggcgcc gcttcgtcgg ccagggtaag caggtcgacc accggcgtgg ggagatctcc
      301 cgcgtatagc ctgaacgccc gcttcttgcc cggcagggtg atcttggcgg agtcgccgga
      361 taccttgatc cgggggaccc ccgcggccac tgtgagctta tagaccccac cgagtgtttc
      421 tccccctgcg gtgaccaacc gtgttccaat cccgaaggag tcgatttcgt gtccctgctg
      481 ccccagcgcc agcagcgttt cctcgtcgat gtcgttgctt gccacgatga tcagcttctc
      541 caggtcgccg ccgttgcgct gtcccgcttc ccggaacagc tgtctcgcct tcttggagag
      601 gaaggagagg tctccggagt cgatccttat ccccatcggc cggtagccca cctctcggag
      661 cgccagagcg acgctcagga agttcggcac accggacctc agtgtgtcat aggtatccac
      721 cagtgccagg aaaccttgag ggtatgcctg agcgtaggct atgaaggagg caagttcgcc
      781 ctcgttggtg cgatttcctc ccaactcctc tcgatagcgc aacaccagct ctaccagatc
      841 gtgttcgcgg ccatccggcc cgacgatgtt gcggctcttg aggtccttca gccccatgaa
      901 gctttggacc caggagtggg cgtgggttcc gacggcgcgg agcccgtcga acagttgagc
      961 tgcttttacg ttgctggtgg agtcgaaacc gccgatgtag gagtaacgag atgccgatac
     1021 acccccgtct ggaccgggcg ctctgcgaag accgaactcc atgagggtct tgtcccaccc
     1081 tgcagccaac cggatcctag ctgctcccgt ggctaccacg ctggggtagt tgaccagcgc
     1141 caggagtggc gcttccagca gctgtactac tgcgacggga ccctcgacga tgaagagggg
     1201 aacacgtggg aaggccaggc tgccctccct taacgccctg atcctgacct tgctgcaatc
     1261 tacggtcttg agccactgga agaaggccgg atcgacatcc gccggcatga agcggcggac
     1321 cacctcgatc tgctcgtcgg tgaaacggta gtccgagacg aatctcagga cctcctcaac
     1381 ccccgcaaag acggtgaact ccccggcgaa ggggttcttc ctgaagtgca catccacgac
     1441 ggcctgttcc tcgtgggtgc cgttgagcca ccaggcgtag gccatggagt actcgtaaaa
     1501 atccgtaagc agggggtcga ccagggggtt tgttggtttc accgtcatct cctacttgag
     1561 ccggatgggt tgtgaggggc gaaacctttc cttgcctgct cacaacccat ccgcgggctc
     1621 cccttctcat cctggaaggc gagggccgat tgatctgtgg attactgtac tgtggccggt
     1681 tcgcggccat cttccgtagc cagcggctgg gcttccgccg agccttcagc cagtggactc
     1741 agtttcttca cgactactat agcgagattg agcagcactg caaagaaggt cgccgagcct
     1801 atggcgtcca gttgggcaaa accgatctcc agggtggtgg gcagcggcgt cgccagcttc
     1861 agcgcgtcgt tcagggcgct gttggtgttg aacaccgcga ccagggccgc gcacaccgcg
     1921 atagagatgc cgtatacgct gaagttctcg atgctacccc aatcctcggt agccgaggag
     1981 atgaccttgg tgctggcacc tgcgatcata ccaaagagaa ccagggtgat gccgcccatg
     2041 accggaagcg ggatggattg gatgagggca ccgaacttag gcaccaggcc cagcaggatc
     2101 gccgtgactg ctgctacctg cagtgggcgg atcgagtaga ccttggtgat ggacatgaca
     2161 ccgatgttct cggcgtaggt agtctggcct gtgccgccac ccatcgcgga gatgaaggtg
     2221 gcgatggcgt cacccatgaa ggcattaccg acctcggggg tcaggtctcg atccatgtag
     2281 gccgagatcg ccttgacgtg gccgatgttc tcggcgacaa gcatgacgac caccggaacg
     2341 atgagcgaca tggcctgaag gctgaaggtc ggagcgacga agttgggtaa tccgatccat
     2401 ggggcggcga caacgccgct gaagtccacc ttccccattg cggccgctac gatgtatccg
     2461 actaccacag cgatgaggat cgggatcagc ttggtgaaac cccgagtacc gaccgcgacg
     2521 attattgcca cagagagagt gatgagtgcc agcgtccagt ccttggacgc gctgccccag
     2581 gctaccggcg cgaggccgat gccgatgacg gcgatgactg ccgcggtgac cggcggcggc
     2641 ataagggtcg cgatgacctt ggttcccgtg tcggttttgg ccagcaggcc gatgatgaag
     2701 tagacgacgg ccgcggctgc gatgccgccc aacgcagcgg gaattccgcc attggccttg
     2761 gcggcgatga cgggaccgat gaaggcgaag gagcttccca ggtaactggg gattcgcccg
     2821 cgaacgacca gcatgaagat gagtgtgccg atgccggaga agaagaccgc cagattcggg
     2881 tcgaagccca tcaggaacgg tgccagtacg gttgccccaa acatcgcgat gacgtgctgc
     2941 agaccgagcc cgatcgttcg c
//
LOCUS       DATALS010000432         5863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_344196, whole genome shotgun sequence.
ACCESSION   DATALS010000432 DATALS010000000
VERSION     DATALS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344196"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..964
                     /locus_tag="VHS06_09570"
     CDS             104..964
                     /locus_tag="VHS06_09570"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX2988407.1"
                     /translation="MRIEKIGVLGAGTMGAGIAQVAALAGYEVQLVDVAQGQLDRALA
                     AIHGNLTRSVEKGRIGTEELDSTLSRLHPATELSSVAGCGVVIEAVQEEFQSKSHLLE
                     SLDSICPPETLFASNTSSISITRLAACTDRPDRMMGLHFFNPVPAMKLVEAVRGLRTS
                     EETRSRLLELVRSLGKTPVEVEDFPGFISNRLLMPMINEAAYALMEGVADRDAIDAVM
                     KLGMNHPMGPLELADLIGLDVCLDIMRVLHDGFGDSKYRPCPLLIKMVAAGHLGKKAG
                     QGFYDYHGRP"
     gene            1152..1667
                     /locus_tag="VHS06_09575"
     CDS             1152..1667
                     /locus_tag="VHS06_09575"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX2988408.1"
                     /translation="MRVRILVAMLVGVVALGLSLVTSGGVALAASSDSSSSLDLSGRW
                     IDANLSTATVTAMEGDTPVYAALSTIGTVNHPTPTGNFTILRRVADETMDSSTIGIPN
                     NAPGGYYLTGVLYTQYFTSYGHSLHYNYWSPASAFGSRAGSHGCLGLTLDDARFFWDF
                     ADVGTPVIIHY"
     gene            complement(2096..2782)
                     /locus_tag="VHS06_09580"
     CDS             complement(2096..2782)
                     /locus_tag="VHS06_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988409.1"
                     /translation="MTKSQLFRISGLALLVGAAAFTVYIVVRSVITAGPDPATFYKEG
                     LWVPINALGVMGSVLVLLGLPGMYARMASRIGSLGLFGVALIALAWMFLGVFLSLFGM
                     LVSPWLADQAPSLVAASAPLPAGFIIAFVSALVIELAGAMLLAIPFIQGRMRPRWVGY
                     VLPASALLTVAGDVVASSGPEANVAMNLLTNMGPVLLLGALGGLGSQESTEHVLSEQA
                     GPREHNPSAG"
     gene            complement(3071..3781)
                     /locus_tag="VHS06_09585"
     CDS             complement(3071..3781)
                     /locus_tag="VHS06_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2988410.1"
                     /translation="MATASATEQLIVSRYPILATYSDLPRPRAYQRCQAALFDRMAKS
                     IEQQVIGSWRARLLASARGRVLDIAAGTGTNLPHYPRGRDLDLVLLEPRSGMLARAAR
                     KARALDRAIELRRGRAEVLPFANGSFDTVVFTYALCTVPDPARALQEAGRVLRPDGRL
                     LLLEHVRAQDPRLARRQDQLAPVQRLLSCGCNPNRDTRATVEAACFAFDLIEEWVELR
                     FSNSIVRPHLLGVARKGT"
     gene            3910..4638
                     /locus_tag="VHS06_09590"
     CDS             3910..4638
                     /locus_tag="VHS06_09590"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX2988411.1"
                     /translation="MIVEPKATPARRSRRSQREGGSGRVPLSRELVLARAIEVGDQEG
                     LEAVSFRRLASDMNVTPMALYRYVRDKDDLLDAMSDMIVGRVDLASVAGAPGGWEGKL
                     RVLLDAFRHVLQAYPVAIPLFTRRAGTTPNWVRTIEVGLGILREAGFPMREAVDLLRQ
                     INNRLAGLVTLEPVAAPSPSPDLDAVVRQSRAILQALQPVEFPNVIEAAPYLTQCDDP
                     ERYYQLGLDLLVDGVRAQLVKFRR"
     gene            4883..5263
                     /locus_tag="VHS06_09595"
     CDS             4883..5263
                     /locus_tag="VHS06_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2988412.1"
                     /translation="MVERRTVSSGSKWEPVVGYSRAVRVGNIISVSGTVGTAADGSIP
                     SDAYSQARNALEIIRRALEEVGARLEDVVRTRMFVTDIRRFDEIARAHCEVFGAIRPA
                     TTMVEVTRLVDPAYLIEIEADAVV"
     gene            complement(5297..>5863)
                     /locus_tag="VHS06_09600"
     CDS             complement(5297..>5863)
                     /locus_tag="VHS06_09600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2988413.1"
                     /translation="NALERAAFTTVATYQAAFLMQSYGLKLDQVAPVLSATAGGTLVG
                     SVIGGRMADGVNRPVLLFTSFQLLAAVAGIPLFLTTPGVVPTAILASVFGLVNSLARP
                     SSMWLLSQVPESRRGATMGFTATSNQVGLMLGASVGGLLISTGGYGLLGFQIPCAAVV
                     SALCCYLGARKMPGRKELAARTALEQPS"
BASE COUNT         1155 a         1723 c         1911 g         1074 t
ORIGIN      
        1 cgactgagga ccagcaggag gggatgaagg ccttcatgga aaaacggcaa ccggagttca
       61 aggggcggtg aagagcgcag tggggtgaga gccgggaggg gcgatgagga tcgaaaagat
      121 cggcgtgctt ggagccggta ccatgggggc cggcatcgcg caggtggcgg ccctggcagg
      181 ctatgaggtg cagttggtgg acgtcgccca ggggcagttg gacagggcgc tggcggcgat
      241 ccacggcaac cttaccagat cggtcgagaa gggtaggata gggacagagg aactcgactc
      301 cacgctttcc agattgcacc cggcgacgga acttagctcc gtcgccgggt gtggtgtggt
      361 tatcgaggcg gttcaagagg agttccaatc aaaatcccac ctgctggaga gcctggactc
      421 catatgcccg cccgaaaccc tgttcgcgtc caacacctct tccatctcaa tcaccaggtt
      481 ggcggcctgc acggacaggc cggaccgtat gatggggctg cacttcttca atccggtgcc
      541 ggccatgaag ctggtggaag cggttcgggg actgcgcacc agcgaggaga ccaggtctcg
      601 actgctggag ttggttcgct cccttggaaa gacacccgtc gaggtggagg acttcccggg
      661 cttcatatcc aaccgattgc tgatgccgat gatcaacgag gctgcctatg cgttgatgga
      721 gggggtggcc gaccgcgatg cgattgacgc ggtgatgaag ctggggatga accaccccat
      781 ggggccgctg gagttggcgg acctgatcgg actggacgtc tgcctggaca taatgcgcgt
      841 gctccacgac ggctttggcg acagcaagta caggccctgt cctctgctga tcaagatggt
      901 cgcggcggga catctgggga agaaggcggg gcagggattc tacgattatc acggtcgtcc
      961 gtgactagat gagggcgggc gccggaggct gaacaaggga atgtagacat aggaacgcag
     1021 cttgccaaca ccggcgggcc tcggaaagga ccggggagac gggtgagcag tgtggctcag
     1081 cggtcgcaca gagacgtgat ggatgagcct cgattgccac ccgcacacca tgaaggagtg
     1141 tgaatgaact gatgagagtt cgaatcctcg tggcaatgct cgtcggtgtg gttgcgctgg
     1201 gactctctct agtgacctcc ggcggggtcg ccttggcagc gagttccgat tctagttcta
     1261 gcctcgacct gtccggtcga tggatcgacg cgaacttgag caccgcgacg gtgaccgcca
     1321 tggagggcga tactcccgtg tatgccgccc tttccaccat cgggacggtg aaccatccga
     1381 ctccaaccgg gaatttcacc atcttgcggc gagtggccga tgagacgatg gactccagca
     1441 ctatcgggat tcccaacaac gcacccggtg gctactacct gacgggggtg ctgtacacgc
     1501 agtacttcac ttcctacgga cactccctgc actacaacta ctggtcgcct gcctcggctt
     1561 tcggtagccg tgccggcagc cacggatgcc tggggctgac gttggacgac gcgaggttct
     1621 tctgggattt cgccgatgtt ggaacgccgg tcatcatcca ttactgacga ttgactcgaa
     1681 agggcggggg tgagccgcaa ggccatcccc tccctttcct ctccctagtg ctctgtcaca
     1741 gccgaatcaa cgaacggtag gggcggacct gtgtgtccgc ctagagcgga gggcggatac
     1801 acaggtccgc ccttgccatt catcggatgc cgtgtgacag agcactagtg ggtcgtttgt
     1861 cactgccgtt gccattcgca gccgcagctc gtcctagaag atggccgcta tctgaccaga
     1921 ggccctggtt ggtgatggcg gtggccgacg cgaatgaccc actagttccc ggagaaggag
     1981 gcgcgtcctc ctactggggt atttgacttc agacccgcgg atgaaggcct ccatcccatc
     2041 acccggaatc acgatccatt atccgagtgt tctaggtcgc agtcggggtg atccctcacc
     2101 cggctgaggg attatgctcc ctcggtccgg cttgctcgct tagaacgtgc tccgtcgact
     2161 cttgagagcc gaggcccccg agtgcaccca ggagtaggac gggacccatg ttcgtgagta
     2221 agttcatcgc aacgttggct tcagggccgc tcgacgcaac gacgtctccg gccaccgtca
     2281 agagcgccga ggctggtagc acatatccca cccagcgggg ccgcatgcgt ccctgaatga
     2341 atgggatggc gagaagcata gcccccgcaa gctcgataac cagtgcggag acgaaggcaa
     2401 taatgaaccc tgccgggagg ggggcagaag cggcgacgag cgacggcgct tggtcggcca
     2461 accaaggcga cacaagcatg ccgaagaggc ttagaaacac accgaggaac atccacgcga
     2521 gcgcgatcag cgcaactccg aacaaaccga gcgagccgat tcggctagcc atcctggcat
     2581 acattccagg aagtccgagg agcacgagca cagagcccat cacaccgagc gcgttgattg
     2641 gcacccagag accctccttg tagaaggtgg ccggatctgg accggcagtg atcacggacc
     2701 ttacaacgat gtagacggtg aatgcagcgg caccgaccag aagggcaagc ccgctaattc
     2761 ggaagagctg tgacttcgtc atctttcctt aacctcacaa cgggttcgcc gcgagccctg
     2821 cgcggcgacc aagaatcctt gacgagacac cgtcgaggcg ggtccgcctg cccgccctcc
     2881 tacccctcct tccacccaaa gaacactgtg ccgttcgggg agcccctggt tcccagagta
     2941 aggggatcgt tgagttctcg acaggctgca atgtagcgat gctgcatagc ccaccattgc
     3001 ccaatagccc tagatgtcga acccaaagcc ggcttggacg cactatcagt tccgagtgaa
     3061 cccgcctccg tcaggtcccc ttgcgggcta ctccgaggag atgtgggcgg acgatggagt
     3121 tcgagaatcg gagttcgacc cactcctcga tgaggtcaaa ggcgaagcag gctgcttcca
     3181 ccgttgcccg agtgtcccgg ttgggattgc agccgcatga gagcagccgt tgtacggggg
     3241 caagctggtc ctgtcggcga gcgagcctcg ggtcctgagc cctaacgtgc tcgagcagga
     3301 gtagccgccc gtccggacgt agcacccgac ccgcttcctg gagcgcccgc gccggatcag
     3361 ggactgtgca caaggcgtag gtaaagacca cagtgtcgaa gctaccattg gcaaagggca
     3421 gaacctcggc tcggccccga cggagttcta tcgcacggtc gagggctcgg gctttgcgcg
     3481 cggcccgagc cagcattcca gagcggggtt ccaggagcac caggtccagg tcccgccctc
     3541 tgggatagtg cgggaggttt gttccggttc cggcggcgat gtcgagcacc cggcctctgg
     3601 ctgatgccag caaccgcgca cgccacgaac cgatgacctg ttgctcgata gacttggcca
     3661 ttcggtcgaa tagcgcggcc tgacaacgct gataggccct gggtctgggg agatcggaat
     3721 atgtggccag gatagggtat ctggatacga tgagttgctc tgttgcggat gcagttgcca
     3781 tgtgcacctc ccctccgggt cgagagtata catcgtaaat atacagcgta tatcagcgtg
     3841 gacaggatag cgtgtacgcc gtatacttgt caagagggca gtggaatagt cgtgtctagg
     3901 aggaatgccg tgatcgttga accgaaggcg acaccagcgc gtcggtcgag gcgttcacaa
     3961 cgcgagggtg ggtccgggcg agtgccgctc agccgggagt tggtccttgc ccgcgccata
     4021 gaagtgggcg atcaggaggg tcttgaggcg gtgagcttcc gccgactcgc ctctgacatg
     4081 aatgtcacgc cgatggccct ctaccgatac gtgcgggaca aagacgatct gctcgatgcc
     4141 atgtcggaca tgatcgtggg acgggtggac ctagcgagtg tcgcgggggc gccgggcggc
     4201 tgggagggga agctgcgcgt gcttctcgac gccttccgcc acgtcctcca ggcgtacccc
     4261 gttgccattc cgctcttcac aaggagggcc ggtacgacac cgaactgggt ccggacgatt
     4321 gaggttgggc tgggcatcct gcgagaggcc ggcttcccta tgcgagaggc ggtggatctg
     4381 cttcgacaga tcaataaccg gttggctggc ctagtcacac tggaaccagt ggccgccccc
     4441 tccccatcgc ccgacctgga tgctgtcgtc cgtcagagcc gcgctatcct acaggccctt
     4501 caacctgtgg agttcccaaa cgtcatcgag gctgccccct acctgaccca gtgcgacgac
     4561 cccgagcgtt actaccagct tggtctggac ctgcttgtcg acggggtgag ggctcaactg
     4621 gtgaagttcc gccggtagct ggctgccccg aactggatcc catcgatgaa gtggagatcc
     4681 gcgtggttca caacggcatt ggcgacttct ggccacgttc cctcgcccgt tagggtacgg
     4741 agggcgaagg gctgccactc gctcccgtcc ggcacggtgc cttgcagcat gtcacggcgg
     4801 tcatcggcga cgtcttctca gcctacggat agagccccgg tgcggccagt gatgggtcct
     4861 agtgggttat taggaggaga gaatggtgga acggcgaacg gtatcgtcag gatctaagtg
     4921 ggagccggtt gtgggctact cgcgtgccgt gcgggtcggc aacatcatca gtgtgtcggg
     4981 gactgtgggc acggcagccg atggctcgat cccctcggat gcctattccc aggctcgcaa
     5041 tgcgctggag atcattcgtc gggcactgga ggaggtcgga gcgcgattgg aggacgtggt
     5101 gcgaactcgt atgttcgtga cggacatcag acggttcgac gaaatcgccc gcgcccactg
     5161 tgaggtcttc ggtgcgatcc gcccagcgac aaccatggtg gaggtaaccc gactggtgga
     5221 tcctgcctac ctcatcgaga tcgaagccga cgccgtagtc tagtcgtcgg agatgctgga
     5281 ccactgtgta agacggttac gagggttgct ccagggccgt tcgggccgcc agttctttcc
     5341 ttccaggcat ctttctggcc ccaaggtagc aacagagggc ggagacaacc gccgcgcatg
     5401 ggatttggaa ccccagcagg ccgtagccgc cggtgctgat gaggaggccg cccacagaag
     5461 cccccagcat caaaccgacc tggttgctgg tggcggtgaa ccccatggtg gcgccgcgcc
     5521 gactctccgg cacctgggag agcagccaca tcgaggaagg tctcgccagg gagttcacca
     5581 gaccgaagac cgacgccagt atggcggtag gcactactcc cggcgtggtc agaaagaggg
     5641 ggattccggc caccgcagcc agcaactgga agctggtgaa gagaagaacc ggccggttta
     5701 ctccatctgc catccttccc ccgatgacgc tgcctaccag cgttccccca gcagtggccg
     5761 agagcaccgg cgcgacctga tccagtttca gcccgtagga ctgcatcagg aaggccgcct
     5821 gataggtggc tacggtcgtg aaggcggccc gctccagggc gtt
//
LOCUS       DATALS010000433         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_344333, whole genome shotgun sequence.
ACCESSION   DATALS010000433 DATALS010000000
VERSION     DATALS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344333"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1236
                     /gene="dsrA"
                     /locus_tag="VHS06_09605"
     CDS             52..1236
                     /gene="dsrA"
                     /locus_tag="VHS06_09605"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HEX2988414.1"
                     /translation="MAEESKTPMLDQLEGGSWPSFAKEIKRRAKKNAAAADLAGQLEL
                     SYEEKRVHWKHGGIVGVKGYGGGVIGRYSDVPDKFPNAAEFHTMRVNMPTGWFYTTEK
                     LRALCDVWEEYGTGLTNLHGSTGDVILLGTQTAKLQDCFNALSEAGFDLGGSGSDLRT
                     PSACVGPARCEYACIDSLGICYDLTLTYQDEIHRPMWPYKFKIKVAGCANDCVASVAR
                     SDMAIVGTWQGPLQINQEAVKAYVAADFPVQSAVVEKCPTRALSLGQNGTMALEAEEC
                     RRCMHCLNMMPKAFRPGSKKGATIMLGGKAPIVRGAYLSSVIVPFMKMEPPYDELKGL
                     LSKIWDWWDENGKSRERVGELIDRLGMGRFLRDMELEPTPQMVFQPRSNPYYFWQPDE
                     VK"
     gene            1238..>2004
                     /gene="dsrB"
                     /locus_tag="VHS06_09610"
     CDS             1238..>2004
                     /gene="dsrB"
                     /locus_tag="VHS06_09610"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HEX2988415.1"
                     /translation="MTEARTDFGPPNYKTMMPPVMADNYGQWLYHETLKPGVLKHVAE
                     SGVAVYSVRVGSPRLLSVDTIREICDIADKFCDGHLRFTSRHNIEFLLTEPEKIEPLI
                     AEVKAHGWPVGGTNNAISNPVHTQGWIHCHSAATDASALVKSIMDEMQPYFESEKLPA
                     KLRISVACCLNMCGAVHCSDLAIVGIHRKPPKVDADRLAKSCEVPNIIAACPTAAIRP
                     NPKEKGVIINEERCMYCGNCFTVCPAMPIADPENDGVA"
BASE COUNT          418 a          606 c          629 g          351 t
ORIGIN      
        1 cgctgggacc attgggcgta ccaacggtgg taacgattag gaggtagctt catggcagaa
       61 gagtccaaga caccgatgct ggaccaactc gaagggggat cctggccgag cttcgccaaa
      121 gagatcaagc ggcgggcgaa gaagaatgct gccgccgcgg atcttgccgg ccagttggag
      181 ctctcctacg aggagaagcg ggtccactgg aagcacggcg gaatcgtggg ggtcaaaggg
      241 tatggcggcg gtgtaatcgg ccgctactcg gacgttcccg ataagttccc caacgctgcc
      301 gagttccaca ccatgcgggt gaacatgccg actgggtggt tctacaccac cgagaagctg
      361 cgtgccctgt gtgacgtgtg ggaggagtac ggtaccggcc tcaccaacct gcacggctcc
      421 accggcgacg tgatcctgct cggaacccag acggcgaagc tgcaggattg cttcaacgct
      481 ctcagcgaag ccggcttcga cctgggcggg tccggatctg acctgcgcac accaagtgca
      541 tgcgtcggcc ccgccaggtg cgagtacgcc tgcatcgaca gtctgggcat ctgctacgat
      601 ctgacgctga cctaccagga cgagatccac cggcccatgt ggccctacaa gttcaagatc
      661 aaggtggcgg gctgcgctaa cgactgcgtg gcctctgttg cccggtccga tatggccatc
      721 gtcggcacct ggcagggacc gctacagatc aaccaggagg ccgtcaaagc ctacgttgcc
      781 gccgatttcc ctgttcagtc ggccgtagtg gagaaatgtc ccacaagagc gctttccctc
      841 ggtcaaaatg ggaccatggc tctagaggcc gaggagtgcc gccgctgcat gcactgcctc
      901 aacatgatgc ccaaggcttt taggcccgga tcgaaaaagg gtgccaccat catgctggga
      961 ggcaaggccc ccatcgtgag aggcgcctat ctgtcctcgg tcatcgtgcc gttcatgaag
     1021 atggagcctc cctacgacga gttgaagggg ctgctctcca agatctggga ttggtgggac
     1081 gagaacggca agtcccggga gcgggtcgga gagttgatcg atcgactggg tatgggccgc
     1141 ttcctccgcg acatggaact ggagccgacc cctcagatgg tgttccagcc caggtccaac
     1201 ccctactact tctggcagcc tgacgaggtg aagtgagatg acagaagcta ggacagattt
     1261 cggccctccc aactacaaga cgatgatgcc gccggtcatg gcggacaact atggccagtg
     1321 gctctaccac gagaccctga agcccggcgt gctgaagcat gttgcggagt cgggagtcgc
     1381 ggtctactcc gttagggtgg gctcgcctcg cctgctcagc gtggatacca tccgcgagat
     1441 ctgcgacatc gcggacaagt tctgcgacgg gcatctgcgt ttcacaagcc gccacaacat
     1501 cgagtttctg ctgacggagc cggagaagat cgagccgctc atcgcagagg tgaaggcgca
     1561 cggctggccg gtggggggca ccaacaacgc tatcagcaat cccgttcaca ctcaggggtg
     1621 gatccactgc cacagcgcgg ccaccgacgc gtccgcgctc gtgaagtcga tcatggacga
     1681 gatgcagccc tacttcgaga gcgagaagct tccggccaag ctgcggatct cagtggcctg
     1741 ctgcctgaac atgtgcgggg ccgtgcactg ctccgatctg gccatagtag gtattcaccg
     1801 aaagccaccc aaggtggacg ccgatcggct ggcaaagagt tgcgaggtgc ccaacatcat
     1861 tgccgcctgc cccaccgctg ccatccggcc gaaccccaag gagaaggggg taatcatcaa
     1921 cgaagagcgc tgcatgtact gcggaaactg cttcaccgtc tgcccggcga tgcccatcgc
     1981 cgaccccgag aacgacggtg tggc
//
LOCUS       DATALS010000434         9608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_116765, whole genome shotgun sequence.
ACCESSION   DATALS010000434 DATALS010000000
VERSION     DATALS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9608
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116765"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(351..1871)
                     /locus_tag="VHS06_09615"
     CDS             complement(351..1871)
                     /locus_tag="VHS06_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX2988416.1"
                     /translation="MLAKVLTCAVVGLDGQLVEVEVDIPSYGMPHFTIVGLPDAAVQE
                     AKERVRAAIKNSGLSFPQRRITVNLAPADLKKEGPAYDLPIATGILMASGQIPMPDGP
                     ALFLGELSLDGSLRHANGILPMVSMARERQIGTVYVPAEDAAEAALVDGLQAIPVSSL
                     ATLVSHLRDEAPIDPMVHRNGWEAEIPADLSRDMLHVRGQEHAKRALEVAAAGGHNLL
                     MVGPPGSGKTLLARTLPTILPAMTVDEMLEVTKIYSVTGRLPSETPLVRERPFRAPHH
                     TISYAGLVGGGKWPHPGEISLAHRGVLFLDEFPEFGQTTLELLRQPVEDGIVTISRAQ
                     GSITFPAKFMLVGAMNPCPCGYFGDPVRECSCSSTLVSKYQKRISGPMMDRIDIHVEV
                     PRIPYDKLAGEVQGESSEQIRSRVEAARSRQRERFAGTNLTCNADMGPANVRTHCHVE
                     GAGQSILKAAVSQMHLSARAYHRILKVARTIADLEGAVQIGAAHVAEAVQYRPRSG"
     gene            2556..5390
                     /locus_tag="VHS06_09620"
     CDS             2556..5390
                     /locus_tag="VHS06_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX2988417.1"
                     /translation="MRILHSKPSVAIVMVLLFISASLGTLVSLGNPALPVTTAPAENG
                     LERVSWVLPASHHWDLGLRAGDVVRRLPGSSPTQVALEVTEGKKVGTVAAAGQSGWSP
                     TAGLIMYLLGLEFFAAGLLVFWKATDRSAASRFALLAEICALTLVFAVGENHGQIAPC
                     ILEWVGTKLTFAAFALFFLTTPVPRWGRLKQAILWALLPLIGLYLYSVVAIPSPYDLV
                     KQFGFMYMLVTMAVSVLALAWPFLTRAPRPQRQLWPVALAIILAAGVFVIGAPVSYLV
                     TGRYLIPPASGIISFAFIPLGFMWAMLRYRIMGVDITPWALLKSVFDTIADPIFVADR
                     HGRLFDANRAGLSLLGVRRPSELVGSFDEQMARLGADSRDSPDPGVSPLQRVLSGSSL
                     HDLSQTLTLPNGEAVFMSVSGSPILDERGEVGLAVLAFRDITERKRAEEALHDSEERY
                     RTVMEAVDDAVYVKDASGRYVTVNTETARRLGMSQQSIPGKTDVELLGPDIGGEIRKH
                     DQSIMEAVRAGSAEEEYPDRVLAPLCQVRKVPLRGEKGEVVGLVSVSRDVTELKSLEE
                     QLRLGQRLEVAGRMAAQVAHDFNNLLGPLVAYPEMIMLKLPPDHPGVTMCEHMLSAAQ
                     QMADINQDMMALGRRGVLEAGPVDLNRVVYESVARLTDVPPSLEVIEDLSPASMLVAG
                     SDVQLHRVMANLIANAREAMNDSGTLTLKTENVYLDQPGGHHCRVETGEYVRLSVSDT
                     GCGIPETILPRIFDAFFSTRRKRRGSGLGLSIVQAIVGDHRGCVDLETAEGVGTTFHI
                     YLPVCREAIADEIPAEIASGNERVLVVDDDVIQREVTVRALGDLGYQVDAVPSGEAAL
                     EYLGKHAVDLLVLDMVMPGGMDGAETYSRVVERQPGIRAIILSGFVESDRVELAQQLG
                     AGAFVRKPASIRRLATAVREELDRTAHVDG"
     gene            complement(5633..7465)
                     /locus_tag="VHS06_09625"
     CDS             complement(5633..7465)
                     /locus_tag="VHS06_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase"
                     /protein_id="HEX2988418.1"
                     /translation="MITRRNFMKTAILAGISLFGDWRGLHQDSMAMPLHQAAEPLDAT
                     TLTKYVDPLPIPDVVQPTRRGSEISYEVSMTQFARRLHRDLPPTTLWGYNGSYPGPTF
                     EARVDQPVTVRWVNSLPTQHLLQVDTRLHGAEQPQNPHVRTVVHLHGGHVPAQSDGFP
                     EAWLTPGDFDVYHYPNNQRATTLWYHDHALGITRLNVYTGLAGLYLLRDEGEAELNLP
                     SRPYEIPIVLQDRSLDTNGQLVYPFPWQPEFFGDAAVVNGMVWPFLEVEPRKYRLRFL
                     DGSSARFYNIKLIDRSTSGDGPPFVQIGTDGGLLPQPVTIEGRLLLGPGERADVVVDF
                     SGFAGRELLLHNNARTPFSGIGPETPPAPDQVPLREIMLFRVKDAVSTPDTSSLPPTL
                     SEFVRLEESSAAAVRDLILAEEVDPEGNVLASLLGVGGGPGGRPLRWGDHITEKPRLA
                     SNEIWQLINLTPDVHPIHLHEIHFQILDRQPFDVDEYKKTGQLVFSPDSPREAPDPNE
                     RGWKDTVRVNPGMVTRIIGQFTGFPGVFPWHCHILDHEDNEMMRPYEIISDMELPRAA
                     DGRALFREPARTQRLFRDTYGERAEAQWITEHNREIGASKTAGS"
     gene            complement(7849..9495)
                     /locus_tag="VHS06_09630"
     CDS             complement(7849..9495)
                     /locus_tag="VHS06_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX2988419.1"
                     /translation="MPIEDLWYKNATIYELNVESFQDSNGDGIGDFGGLTRRLGYLAG
                     LGVTCLWLAPFYPSPRRDDGYDIVDYLNVDRRYGTLGDFVEFIHEAESFGIRVIADLV
                     INHTSNEHPWFQAARADPSSRCHDYYVWSEKKPADITSGVVFPGYQESVWSYDEAAGA
                     WYYHRFYDFQPDLNYGNPDVREEVHKIMGFWLQLGVSGFRVDAVPYVLEVKGPDMPNG
                     SRDYGYLEDIRQFLTWRSRDAIMMGEANVPLSELPHYFGSGNRMQMLLNFMENQHLFN
                     SLAQENATPLIRAMETLPKLPPACQWGTFLRNHDEIDLGRLTEAERRRAFQLFGPEPS
                     MQLYGRGIRRRLAPMLGNDQRRIELAYSLLFTLPGTPILRYGEEIGMGDDLSLEQRDA
                     IRTPMQWSAERNGGFSSAPREKLVRPVITGGEYGYERVNVERERLDPNSLLNRIERMI
                     RLRKEHPEFGRDQWEVVDTGQAEVFAIRASSKGSTMVAVHNFSGSKVKFRLELPGTED
                     QRLTDTLKGEELGTSKEGACEIELDGYEYRWLRVARALDA"
BASE COUNT         1737 a         2865 c         3001 g         2005 t
ORIGIN      
        1 tctggtggtg ccaacggacc tgggcccgcc aagtaccacc agcgatgtaa gtatcgttgg
       61 ctgtggcgga aggagaagac ggtccgggag tgcacaagac acggcactca taattacagg
      121 ccaatgtagt tagtgcacgc atggcaccca tgcaggtttc tgttgcaatc ttgagcggac
      181 ctacccgacc ccatccttgc gagagagttt tgggccgcga ggcgcagcat gcctacgcct
      241 cgttcgggtc tagtttccct agcagtcccc tatgggaagg tctacgcaat cggaggctgg
      301 aactctatcg atctgaggac catccgactg ggacagatcc agtcgtggga ttatccgctt
      361 ctcggcctgt actgcactgc ctccgcgacg tgggcggcgc cgatctggac cgcgccctcc
      421 aggtctgcga tggtgcgggc gactttgagg atgcggtggt aggcgcgggc ggagaggtgc
      481 atctgcgaga cggcggcctt gaggatgctc tggccggccc cttccacgtg gcagtgggtc
      541 cggacgtttg cggggcccat gtccgcgttg caggtgaggt tggttcccgc aaagcgctct
      601 cgctggcgcg acctggcagc ctctacacgt gagcggatct gctccgagct ttccccctgg
      661 acctccccgg ccagcttgtc ataagggatg cgcgggacct ctacgtggat gtctatgcgg
      721 tccatcatcg gccccgagat ccgcttctgg tacttggaga ccagggtgga ggagcaggag
      781 cactccctga cgggatcccc aaagtagccg caggggcacg gattcatggc tccaaccaac
      841 atgaacttgg cagggaaggt gatgctgccc tgggcgcggg agatggtaac gatgccatcc
      901 tccaccggtt ggcgcagcaa ctccagggtg gtttggccga actcagggaa ctcgtccagg
      961 aacagcaccc cgcggtgggc cagggagatc tctccggggt ggggccactt gccgccaccg
     1021 accaggccgg cgtaggagat ggtgtggtgg ggggctcgga agggacgctc ccgcaccaat
     1081 ggggtctcgc tgggcagccg gccggtgacg gagtagatct tggtgacctc cagcatctca
     1141 tcgacggtca tggccggcag tatggtcggg agggtgcggg ccaggagcgt ctttcccgag
     1201 cccggcggtc cgaccatcag gaggttgtgg ccgcctgccg ctgccacctc cagggctcgc
     1261 ttggcgtgct cctgcccccg gacgtgcagc atgtccctgg agaggtcagc ggggatctcc
     1321 gcctcccagc cattgcggtg gaccatcggg tcgatggggg cctcgtctcg aaggtgggag
     1381 acgagggtgg ccagactgct caccggtatg gcctgcagac catccaccag cgccgcctca
     1441 gccgcgtcct cggccggcac gtataccgtc ccgatctggc gctccctggc catggagacc
     1501 atgggtagga tcccgttggc atggcggagg gacccgtcca gcgacagctc acccaggaag
     1561 agtgcggggc cgtctggcat cgggatttgg cctgaggcca tgagaatgcc ggtggcgatg
     1621 ggcaggtcgt aggcaggccc ctctttcttt agatcggcgg gggcaaggtt gaccgtgatg
     1681 cgccgctggg ggaaggagag gccggagttc ttgatggccg cgcgcacacg ctccttggcc
     1741 tcctgaacgg ccgcgtcggg gaggccgacg atggtgaagt ggggcatgcc gtaggagggg
     1801 atgtcgactt cgacctcgac gagttggcca tcgaggccga cgacggcgca agtgagcacc
     1861 ttggcgagca tgggacctcc agaaggcaca agacaataga cgtcaagcta cgtccgcatc
     1921 atagcatgaa cgttgttgtt taagaagagc catcttcggt gtgtgttcgg tcctagttgt
     1981 gtcggttctt gggatcgcct tcagacgtgt ctgcggtcat actgctccgg cagctcacat
     2041 agattcagga gcgttgtttg cattgactgc gttccctgtc tatggacgct atcaggcact
     2101 accgatggca tggaggcagt gactgccact gctcgaaatc gctcaccgcg ggcttttcca
     2161 cagacctgcc accaacattg tgccccacgt caaccttgct tgatttcacg cctgtagctt
     2221 tcatagcgat atctgctata tacttcatgc ggtttgccag gcaaagtctg attgtggccg
     2281 cgttgcgccg tttcccgcgt accgccgtca tcgatccaag gccggagtac ctcggcacgc
     2341 tcggaacctc gtcaactacc accaatacgc cacaagcgcc acttgcgcgc ccatcagctg
     2401 cggctcgctc ctcaaatctt gccatcttgc cgtagcgaga gcggccttac ctttgtatgg
     2461 caagctggcg tgcacaaagg cggctcggca gtctgcgccc gtatccgaat cgtgagcccc
     2521 caccactagg ctaaggttga gtgaggttga tatcaatgag gattctccac tccaaaccct
     2581 cggttgccat tgtgatggtt ctactgttca tatcggcatc ccttggcaca ttggtgtcgc
     2641 tcggcaatcc cgcgctgcct gtcaccaccg ctcctgctga gaatggccta gagagggtct
     2701 cgtgggtgct gcccgctagc catcattggg atctgggact tcgggccggc gatgtggtgc
     2761 ggcgccttcc cggcagttcg ccaactcagg ttgcgctgga ggtaactgag gggaaaaagg
     2821 tgggcaccgt cgccgcagcg ggtcagtcgg gctggtcccc gacagcgggg ctgatcatgt
     2881 acctgcttgg attagagttc ttcgctgccg gtctactcgt gttctggaaa gcaacggacc
     2941 gctctgcggc aagtcgattc gccctgctcg ccgagatatg cgcgctcacg cttgtattcg
     3001 cggttggtga gaaccacggt cagatcgccc cttgcatttt ggagtgggtt ggcaccaaac
     3061 tcacctttgc tgcctttgcc ctcttcttcc tcaccactcc tgtgccccgc tggggacggc
     3121 tgaagcaggc gatcctgtgg gcgcttctac cacttatcgg cctttacctg tactctgtgg
     3181 tcgcgattcc ttcaccctac gacctggtca agcagtttgg cttcatgtac atgctggtca
     3241 ccatggcggt cagcgtcctg gcccttgcct ggccctttct gacccgagca ccccggcctc
     3301 agcgtcaact gtggccggtc gctctcgcga tcattctggc ggcgggggtc ttcgtgatcg
     3361 gcgctccggt ctcctacctc gtgacgggac gctacctcat ccctcctgcc tccggcatca
     3421 tctccttcgc cttcataccc ctcggcttta tgtgggcgat gcttcgttac cggatcatgg
     3481 gtgtcgacat caccccctgg gcgctcctga agagtgtatt cgacaccatt gccgacccca
     3541 tcttcgtggc ggaccgtcac gggcggctct tcgatgccaa cagggcggga ctctcgctcc
     3601 tgggggttcg caggccttcc gaacttgttg gttccttcga tgagcagatg gcgaggttgg
     3661 gcgcagatag ccgggactcc ccagaccctg gagtctcccc tcttcaacga gtgctgtctg
     3721 gatcgagcct gcacgacctc agccagacgc tgaccctgcc taatggagag gctgtcttca
     3781 tgagtgtttc cgggtctccg atcctggatg agcggggcga ggttggcctg gcggtgctgg
     3841 ccttccggga catcaccgag cggaagaggg cggaggaggc gctgcacgac agcgaggaac
     3901 ggtatcggac agtgatggaa gcagtagatg acgctgtcta cgtgaaggac gccagtggtc
     3961 gctacgtgac tgtcaacacc gaaacggcca ggcggctcgg gatgagtcag cagtctatcc
     4021 caggcaagac cgacgttgaa ctgctcggac ccgacattgg aggggagatc cgaaagcacg
     4081 accagtccat catggaggct gtgagggcgg gcagcgccga ggaggaatac cccgacaggg
     4141 ttctggctcc actgtgccag gtccgcaagg ttcctctcag gggcgagaag ggtgaggtgg
     4201 tcgggctggt ctcggtttcc agggacgtca ccgaactgaa gtccctggaa gagcagcttc
     4261 ggctgggcca gaggctggag gtggccggcc ggatggctgc ccaggttgcc cacgacttca
     4321 acaatcttct cggcccactg gttgcctacc cggagatgat catgctcaaa ctcccaccgg
     4381 accacccggg cgtcaccatg tgcgagcata tgctctctgc agctcagcag atggcagata
     4441 tcaaccagga catgatggcc cttggccgta ggggtgtttt agaagctggg cccgttgatc
     4501 tgaacagggt ggtgtacgag agtgtggcac gcctcacgga tgtgcctcca agcctcgaag
     4561 tcatcgagga cctatccccc gcatcgatgc tggtggcggg ctccgatgtc cagttgcacc
     4621 gtgtcatggc caatctcatc gctaatgcga gggaagcgat gaacgattcg ggaaccctga
     4681 ccctcaagac cgagaacgtc tatctcgacc aaccaggggg ccatcattgc cgagtggaaa
     4741 ctggggaata cgtgaggctc tccgtctcgg ataccggctg cggaatccct gaaacgatcc
     4801 ttcccaggat ctttgacgca ttcttctcga ccaggcggaa gaggcgagga tctggactgg
     4861 ggttgtccat cgtccaggct atcgtaggtg accaccgggg atgtgtggac ctggagaccg
     4921 cggagggagt cggcaccacc ttccacatat acctgccggt gtgcagggag gccattgctg
     4981 acgaaattcc agcagagata gccagcggaa acgagagggt gctggtcgtt gacgacgatg
     5041 tgatccagcg agaggtgaca gtgcgggccc ttggggatct tggatatcag gtcgatgcag
     5101 tccccagtgg agaagcggcc ctggagtatc tgggtaagca tgctgtagac ctgctggtcc
     5161 tcgacatggt gatgccgggg ggaatggacg gcgcggagac gtactctcgc gttgttgaga
     5221 gacagccagg catcagagct atcatcctat cgggtttcgt ggagtccgac agggtggagc
     5281 tggcgcagca actaggagct ggagcctttg tgcggaaacc cgcgagcatc aggcgactgg
     5341 cgactgcggt cagggaggag ctggatcgga cggcgcacgt ggacggctga tctcatattg
     5401 tgccgacacc gtaccactca tcatggggac gcttggtgca gactcgctcg cgcagttcca
     5461 ccaccaccgt catgggggac tgatcctgcg gttaagagca gcagatagcc gccctgctcg
     5521 ttgatcaaga cggctgatgc gtcacggtac aaagagcgac ttggcccttg tcaggacgat
     5581 gaacttgtgg acgaagacgc tctcgtcgag gccgatatca attatggccc gctcacgagc
     5641 ctgccgtctt agatgctcct atctcccgat tgtgttccgt tatccattgt gcttcggcac
     5701 gctcgccata ggtatctctg aagagacgtt gagtcctagc gggttctcga aacagcgccc
     5761 gcccgtccgc cgccctgggt agttccatgt cggagatgat ctcgtacgga cgcatcatct
     5821 cattgtcctc gtggtccagg atgtggcaat gccaggggaa tactcccggg aagcctgtga
     5881 attgaccgat gatcctggtg accattcctg ggttcacccg caccgtgtcc ttccaacccc
     5941 tttcgttggg atccggagcc tcgcgcgggg aatccggcga gaacacaagt tgcccggtct
     6001 tcttgtactc gtccacgtcg aagggttgac ggtcaagaat ctggaagtgg atctcgtgaa
     6061 ggtggatggg atgcacatcg ggcgtgaggt tgatcagttg ccatatctcg ttggacgcga
     6121 gcctcggctt ctcggtgata tggtcacccc agcgtaaggg ccgtcctccg ggaccgccac
     6181 caacccccag cagcgacgcg agcacgttgc cctcgggatc gacctcctcc gccaggatca
     6241 ggtcacgcac tgcagcggcc gaactctcct ccagtctcac aaactccgat agggtggggg
     6301 gcagcgaact ggtgtccgga gtcgatactg catccttcac cctgaagagc atgatctccc
     6361 gaagaggcac ctggtccggc gctggcggcg tctccggacc gatccccgaa aagggggttc
     6421 tggcgttgtt gtggagaagc agttctctcc ccgcgaagcc ggagaaatca accaccacgt
     6481 cggctcgctc gccggggccg agcagaagcc taccctcgat ggtgacaggc tgtggaagca
     6541 gtcctccatc ggtcccaatc tgcacgaagg gaggcccatc gccgctggtc gatcggtcga
     6601 tcagcttgat attgtagaag cgggcgctgg atccatccag gaatctcagc ctgtactttc
     6661 tcggctctac ctccaggaac ggccagacca tgccgtttac gacggccgcg tctccaaaga
     6721 actctggttg ccaggggaag gggtagacta gttgaccatt ggtgtcgagg gaccgatcct
     6781 ggagcacgat cgggatttca tagggcctgc tgggaaggtt aagttcggcc tccccctcgt
     6841 ccctcaacag atacagtccg gcgagccccg tgtagacgtt cagccgggtg atgccgagag
     6901 cgtggtcgtg gtaccagagg gtagtggccc tctggttgtt gggatagtgg taaacgtcga
     6961 aatccccggg ggtgagccat gcctccggga acccatcact ttgtgccggc acatgccccc
     7021 cgtggaggtg taccacggtc cgcacgtgcg gattctgggg ttgctccgcc ccatgaagtc
     7081 tggtatccac ctgcagtaag tgctgtgtgg ggagggagtt gacccacctg accgtgacgg
     7141 gttggtctac tctagcttcg aaggtggggc cgggatagga accgttgtac ccccataggg
     7201 ttgtaggggg aaggtcacga tgcagccttc gcgcgaactg tgtcatggag acttcgtagc
     7261 tgatctctga cccacgtcgc gtcggctgca cgacgtccgg tatgggtagc gggtccacgt
     7321 attttgtgag tgtcgtggca tctagcggct cggcagcctg gtgcagcggc atcgccatcg
     7381 agtcttggtg caggccacgc cagtccccga agaggctgat cccggctaga atggcagtct
     7441 tcatgaagtt gcgtcgggtt atcattcgtc gtctcctgaa gatgggcgaa ctgccgttgc
     7501 ctagccacca ggcccctcgg tcaggtggcc ccactgcatg gtccagcctt cttcgacagc
     7561 ggtgacggga ctctctgcct cgcgaagaca gggccagttg acgatgcttt ggatgtcttc
     7621 ctgatcaact tgcagcccac gtgccactcc ttcacctggc tctttgctgc aggagctagg
     7681 cgactatcga gcggtactat ccgactcacc ggtagagccc ctgccaatgc acagtagggg
     7741 cggacttgcc agtaaaaccc taccttcggt tgtctcagcg gttacgcaac atgtttgcag
     7801 agtagcccga tgccggcggc tttggatggg tgtacacgta tccagagctc aagcgtcgag
     7861 ggcgcgggct accctgagcc agcggtactc gtagccgtcc agttcgatct cgcaggcccc
     7921 ctccttcgat gttcccaact cctctccctt cagcgtgtcc gtgagccgtt ggtcttctgt
     7981 gcccggcagt tccagccgga acttcacctt cgatcccgag aagttgtgca ccgccaccat
     8041 agtgctgccc ttcgagcttg cccgaatagc gaagacctcc gcctggcctg tgtcgacgac
     8101 ctcccactga tcgcggccga attccggatg ctccttccgg aggcggatca tccgctcgat
     8161 ccggttcagg agcgagttcg ggtccagccg ctccctctcc acgttcaccc tctcgtagcc
     8221 gtactctccg ccggtgatca caggcctcac cagcttctcc cggggggccg aggagaaacc
     8281 cccgttgcgt tcagccgacc actgcatcgg agtacgaatg gcgtcacgct gctccagcga
     8341 caggtcatcc cccatgccga tctcttcccc gtatcggagg atgggtgttc cgggaagcgt
     8401 gaagaggaga ctataggcca gctcgatgcg tcgctggtcg ttgccaagca tcggggcgag
     8461 ccttcggcgg atgcctcgac cgtagagctg catggacggt tccggcccga acagctggaa
     8521 ggcgcgtcta cgttccgctt cggtcagccg tcccaggtcg atttcgtcgt ggttcctcag
     8581 aaatgttccc cactggcagg cgggaggtag cttgggcagg gtctccattg ctcgtatcag
     8641 cggcgtcgcg ttctcctggg ccagggagtt gaacaggtgc tggttctcca tgaagttgag
     8701 cagcatctgc atccggttgc cgctcccaaa gtagtgcgga agctcgctca aggggacgtt
     8761 cgcctcgccc atcatgatgg catcccgcga gcgccaggtc aggaactgcc ggatatcctc
     8821 cagatagccg tagtctctcg acccgttggg catgtccggc cccttgactt ccagaacgta
     8881 aggcacagcg tctaccctga aaccggagac acccaactgg agccagaagc ccattatctt
     8941 gtgtacttct tcgcggacgt ccggattacc gtagttcagg tcgggctgga aatcgtagaa
     9001 gcggtggtag taccaggctc cggccgcttc gtcataggac cagaccgatt cctgataacc
     9061 cgggaacacc acaccggagg tgatgtctgc cggcttcttc tccgaccaga cgtagtaatc
     9121 atggcatctg gacgaggggt ctgcccgtgc cgcctggaac caggggtgct cgttggaggt
     9181 gtggttgatg accaggtcgg cgatgaccct gatgccgaag ctttccgcct cgtggatgaa
     9241 ctccacgaag tcgcccaggg tgccgtagcg gcggtctaca ttaagatagt ccacgatgtc
     9301 gtacccgtcg tcgcggcgcg gcgatgggta gaatggggcc aaccagaggc aggtgacccc
     9361 gagtccggcc aggtatccga gccttctggt caggcctccg aagtccccaa tgccgtcccc
     9421 attggagtcc tggaacgact cgacgttgag ttcgtagatg gtggcgttct tgtaccagag
     9481 atcctcgatc ggcatttcgc actcttccca ctgttgtgtc gccctgcagt gaggaagcaa
     9541 ggcacatacc ctcagactgg tttccgaata cctactcacc tgttacactg caacaacatc
     9601 taccttta
//
LOCUS       DATALS010000435         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_117000, whole genome shotgun sequence.
ACCESSION   DATALS010000435 DATALS010000000
VERSION     DATALS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117000"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..2096
                     /locus_tag="VHS06_09635"
     CDS             180..2096
                     /locus_tag="VHS06_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX2988420.1"
                     /translation="MPCGYTGKLLKIDLSRGSWAVEQPDEKFYRRYWGGKGLAGYFLL
                     REQVAGVDALGPDNLLIFATGLMTGAALPAMPRFTVAAKSPLTGMYGESEAGGWWGPE
                     LKFAGYDAIILKGQADRPVYLWIHDGEVEIRDACSLWGQGTVEAQEAIRAELGDPRIR
                     VAGIGQAGERMVNYACIINEGKHANGRTGMGAVMGSKRLKAIAVRGHGRLQSFDREAT
                     QELARWYAAECATNPTTMVLSDTGTAGLVSGFQAGGMLPTRNFSHGTFDAAGQIGWES
                     MERELFQEKKGCFACPIRCKRVIRSEGAFQIDPRYGGPEYETIGAFGSDCGIGDLRVV
                     CKANEMCNRYGMDTISCGTTIAFAMECFENGLLSREECDGLELCFGNAEAMLAMVDKI
                     AQREGIGDLLAQGSAKAAAKIGRGAERFAIQVNGQELPMHEPRGKQGVGLGYAVSDTG
                     ADHLVIAHDPAFQMKESLILKTMEPFGILEPISALDLGPAKVRLFCYLEDINSLWKAA
                     GVCNFGFAPRVAIPFEKFVEMFRAQTGWDVSSFDMLKIGERCTNLSHAFNVREGRRRS
                     GDRLPERFYEPLPDGRLKGVAIDRGAFEEALTLLYQMKGWNPDTTIPTRAKLLELELD
                     WVVDELQAAGVPMQ"
     gene            2220..3533
                     /locus_tag="VHS06_09640"
     CDS             2220..3533
                     /locus_tag="VHS06_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX2988421.1"
                     /translation="MKVCDTHTPEQTIASHFQEAVRWRRDLHRHPQPAWLEFYSTGMV
                     AEKLAGWGYRLQLGREIVAPERQLLLPAPKVLEVEYRRAIEAGIQEQFVAPARGGFTG
                     VVATLDGPEPGPTVAFRFDIDSNEVMESADPSHRPAREGFASQYPGYAHACGHDAHTA
                     IGLLLARHFAENRDRIRGSVKFIFQPNEENLSGALAMVDMGLLDGVDYLFGAHVGLNV
                     TETGLVGVDVRDFLALARFEVTYTGRATHAAARPDEGKNALLGACAAVTNLYAISRHG
                     LGASRVNIGTFEAGTTWNVIPDRATFRMETRGATDEINAFMARRARDVLEGAARMYDL
                     GLEVKPAATALSRQSSPDMVELASSVAGTLPSVKGIAREIPFNASEDVTVMMERVQKR
                     GGKALFLLLGTPTFGGHHSTTFDIDETVIRNGAELFAALYERVTG"
     gene            complement(4067..4927)
                     /locus_tag="VHS06_09645"
     CDS             complement(4067..4927)
                     /locus_tag="VHS06_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HEX2988422.1"
                     /translation="MSGKDRRLLQEGAVNFCYCDESGTGEEPIATMAGIVVDAGRMHL
                     TKADWRDLLERLRERTSRHIDELHTADFYAGNGVWRGMNGPDRAFVINEIFEWLAKRK
                     HHIVYASVNKACYGKARQQNGIPDGLGTVWRFLGFHLVLAIQRYSQPEKKRKGNTYLV
                     FDNEEQEHLRFPELILTPPAWSDEYYGRDKKDEALSEIVDVPVFGDSKTLPLLQVADL
                     VAFFLRRYAEMQESLLPAKYNGEKDKVSGWVRQIQARSIGRAHIYPKSKRNSAQDLFY
                     QMAPPSLRDL"
BASE COUNT          995 a         1612 c         1595 g          873 t
ORIGIN      
        1 cacggtgaag cgtggcatgg cgggcagggc ggcgccggtc atgaggccgg gtggccgtga
       61 ccgcgccgtg taagcggggc acaagacccc gccctacgag acggcgctgc gggcgtctcg
      121 cccgcccgtt cccgtcgaag aagcgaatca cttgtcgatc gctcatttcg gaggcaacga
      181 tgccctgcgg atacacaggc aagttactaa agatagacct ctcccgcggc agttgggccg
      241 tcgagcagcc ggacgagaag ttctaccggc gctactgggg aggcaagggc ctcgccggct
      301 acttccttct tagagaacag gttgcgggag tcgacgccct cggtccggac aacctcctga
      361 tcttcgccac cggcctcatg accggcgccg ccctgcccgc catgccacgc ttcaccgtgg
      421 cggcaaaatc ccctctcaca gggatgtacg gggaatcgga ggccggaggc tggtgggggc
      481 cggagctcaa attcgccgga tacgacgcca tcatcctgaa agggcaggcg gatcgccccg
      541 tctatctctg gatccacgac ggcgaggtgg agatacgcga cgcgtgcagt ctgtggggtc
      601 agggcaccgt cgaggcgcag gaggcgatcc gagccgagct gggcgatcct cgcatccggg
      661 tggcggggat aggacaggcc ggcgagcgga tggtgaacta cgcctgcatc atcaacgagg
      721 gcaagcacgc caacggtcgc accgggatgg gcgcagtcat gggatccaag cggctcaagg
      781 ccatcgccgt gaggggtcac ggccggctgc agagcttcga cagagaggcc acgcaggagc
      841 tcgcccgatg gtatgcggcc gagtgtgcga cgaaccccac caccatggtc ctctccgaca
      901 ccggcacggc cgggctcgtg agcggatttc aggccggggg catgctgccc acccgcaact
      961 tcagccacgg caccttcgac gcggccggcc agatcggctg ggagtccatg gagcgggagc
     1021 tgttccagga gaagaagggc tgcttcgcct gccccatccg ctgcaagcgg gtgatcagat
     1081 ccgagggagc cttccagatc gacccccggt acggcgggcc ggagtacgag accatcgggg
     1141 ccttcggctc cgactgcggc atcggcgacc tccgcgtcgt ctgcaaggcc aacgaaatgt
     1201 gcaaccgcta cgggatggac accatctcct gcgggaccac cattgccttc gccatggagt
     1261 gtttcgagaa tgggctgctc agccgggagg agtgcgacgg actggagctg tgcttcggca
     1321 acgccgaggc gatgctggcc atggtggata agatcgcaca gcgggagggt atcggcgacc
     1381 tgctcgcaca ggggagcgcg aaggccgcgg caaagatcgg gcggggtgcc gagcgctttg
     1441 ccatccaggt gaacggccag gaactgccga tgcacgagcc gcgaggtaag cagggggttg
     1501 ggctgggcta cgccgtctcg gataccggcg ccgaccacct ggtcattgcc cacgacccgg
     1561 ccttccagat gaaggaatcg ctgattctaa agaccatgga gcccttcggc atactggagc
     1621 cgatctccgc cctggacctg gggccggcga aggtccggct gttctgctat ctggaggaca
     1681 tcaacagcct ctggaaggcc gcgggcgtct gcaacttcgg cttcgccccc agggtggcga
     1741 tccccttcga gaagttcgtg gagatgttca gggcccagac gggctgggac gttagcagct
     1801 tcgacatgct gaagataggc gagcgctgca ccaacctctc ccacgccttc aacgtccggg
     1861 agggccgacg caggagcgga gaccggctgc ccgagcgctt ctacgagccg ctccccgacg
     1921 gtcgcctgaa gggggtggcc atcgacaggg gcgccttcga ggaggcgctg acgctgctgt
     1981 accagatgaa ggggtggaac ccggacacca ccatccccac cagggcaaaa ctgctggagc
     2041 tcgagctgga ctgggtggtg gatgaactgc aggcagccgg cgtaccgatg cagtagcccg
     2101 cgacccacgt gaatagtagc gctgaccccc gctactattc acgtggatgt agctatgcca
     2161 caatacctcc cgaggattgg cttcggccac gatccaccag cacgagggag gttggcccga
     2221 tgaaagtctg cgacacccac acccccgagc agaccattgc ctcgcacttc caggaggccg
     2281 tccggtggag gcgcgacctg caccggcacc cgcagcccgc gtggctggag ttctactcca
     2341 ccggcatggt ggcggagaag ctcgctggct ggggctaccg cctgcagctg ggcagggaga
     2401 ttgtcgcccc cgagcggcag ctgctcctcc ctgcgcccaa ggtccttgag gtcgaataca
     2461 ggcgcgccat cgaggcgggc atccaggagc aattcgtcgc cccggcgcgg ggaggcttca
     2521 ccggcgtagt cgccacgctg gatgggccgg agcccggccc aacggtcgcc ttccgcttcg
     2581 acatcgactc caacgaggtg atggagtcgg ccgatcccag ccaccgccct gcccgggagg
     2641 gcttcgcctc gcaatacccc ggctacgccc acgcctgcgg gcacgacgcc cacaccgcca
     2701 tcgggttgct gctcgcccgc cacttcgccg agaaccgcga caggatcagg ggcagcgtca
     2761 agttcatctt tcaacccaac gaggagaacc tctccggcgc gctcgccatg gtggacatgg
     2821 ggttgctgga cggcgtcgac tacctcttcg gcgcccacgt cggcctgaac gtgacggaga
     2881 cggggctggt gggggtggac gtccgcgatt tcctggccct ggcccgcttc gaggtcacct
     2941 acaccgggcg cgcgacccac gctgccgcgc ggcccgacga ggggaagaac gcgctgctgg
     3001 gcgcctgcgc agccgtcacc aacctgtacg ccatctcccg ccacgggctg ggggcatccc
     3061 gggtcaatat cggtaccttc gaggcaggca ccacctggaa cgtcatcccg gatcgggcca
     3121 ccttccggat ggagacccgg ggcgcgaccg acgagatcaa cgcgttcatg gcccgcaggg
     3181 cgcgggatgt gctggagggc gcggcccgga tgtacgacct gggcctggag gtgaagcctg
     3241 cggccaccgc gctgagccga cagagctcgc ccgacatggt ggagctggcc tcctcagtag
     3301 ccggaacgct cccctccgtt aaggggattg cccgagagat ccccttcaac gcctcggagg
     3361 acgtgaccgt gatgatggag cgggtccaga agcgcggcgg caaggcgctg ttcctgctgc
     3421 tcggcacccc caccttcggg ggccaccaca gcaccacctt cgacatcgac gagacggtga
     3481 tccgcaacgg cgccgagctg ttcgccgcgc tgtacgagag ggttacgggg tagggcaggt
     3541 gcctaacggc agcaatccag acgaggactt ggggaagagg cttcagaact cgggtatcag
     3601 cagcgattcg aaggggccag tagagtctga ggcatgtttc tacaattggc tgcactcacg
     3661 gaaggtcgcg gacttgtttt aagcgaagca ggggacattc accaagggta gcagatcact
     3721 ttggccgcag gatttgccga ttgggattgg agaatcatcg ccagcacact gacctcgtcc
     3781 ctggcgtcga gcacattccc gatatgcctg gacgttcaag cagtaaacgg ccaactgaca
     3841 tcagcgaact ggcgctgatc atcgctgacg aagctacgaa caacatcctc gcatccaagg
     3901 acacgacacc acacgaaaca taccagagga actcggcagc catcgcgctc ggcaagctgg
     3961 gcacaacaga gagtggccct gtcagagcgg cagggctcac aacaaaggag aagcaagagc
     4021 tcgccaagaa ggcggcgcaa gaacgctggt cgccacgcac aagttgtcat agatcacgaa
     4081 gcgagggcgg agccatctga tagaacaaat cctgtgcact attccgcttt gacttaggat
     4141 agatatgcgc tcgaccgatt gagcgagcct gtatctgcct aacccatccg cttactttgt
     4201 ctttctcacc attgtatttg gccgggagca atgactcctg catttccgcg taccgccgta
     4261 agaaaaacgc cacaagatcc gccacttgga gcagcggtag tgtcttggag tcgccgaata
     4321 ctggaacatc gactatctcg ctcagcgcct cgtctttctt gtcacgacca tagtattcgt
     4381 cgctccatgc cggtggggtc agaatcaact ctggaaacct caggtgttct tgttcctcat
     4441 tgtcgaacac aagataggtg tttcccttcc tcttcttctc gggttgggag taccgctgga
     4501 tagctaggac aaggtgaaag ccgagaaacc gccagacagt tcccaatcca tctggtatcc
     4561 cgttttgctg acgtgccttg ccatagcagg ccttattcac tgatgcgtag acaatgtgat
     4621 gcttcctctt agccagccac tcgaaaatct cgttgatcac gaaggctcgg tcgggaccat
     4681 tcatgccccg ccacacgcca ttgccggcat agaaatctgc cgtatgcaac tcgtcaatgt
     4741 ggcgtgatgt tctctccctg agtctttcca ataggtctcg ccagtccgcc ttggtaaggt
     4801 gcatgcgacc agcatcaacc acaatgccag ccatcgtggc tattggctcc tcgcccgtac
     4861 cactttcatc acagtaacag aagttcacag caccttcctg gaggaggcgt ctatctttgc
     4921 cggacattat cgtcctccaa ggtgtgctgc ctcaaggcaa ttgttgtcct aacgtcgccc
     4981 ctcagaaaac ccgcacacac cagttctccc ccctcccgtg gggtctgctg gtgtccctct
     5041 tccgtgaaca ggagatctcc caggtccaca ttccc
//
LOCUS       DATALS010000436         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_435833, whole genome shotgun sequence.
ACCESSION   DATALS010000436 DATALS010000000
VERSION     DATALS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435833"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VHS06_09650"
     CDS             <1..304
                     /locus_tag="VHS06_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein prkA"
                     /protein_id="HEX2988423.1"
                     /translation="GVTENAKKACREEILIRISALARRGQRLDYRSHERLKEAIEKKL
                     FADLRDVVKITTSTKTPDAEQLKRINDVVDRLVKEQGYCHVCANDLLNYVGTLLNR"
     gene            441..1601
                     /gene="yhbH"
                     /locus_tag="VHS06_09655"
     CDS             441..1601
                     /gene="yhbH"
                     /locus_tag="VHS06_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HEX2988424.1"
                     /translation="MGNFTISLSKHDWSLHRKAPQDQARHNEKVREAIKSNLADIVAE
                     EAIVTSDGNRAIKVPIRSLELPHFRYDTNRKDHLGQGNGDSQVGDVLGQEAGQDGPGN
                     GKGAGDQPGLDYYEAEITIDELADLLFEDMGLPNLEQKQAQQIESTIIRFNDIRKSGP
                     MSSLDKRRTVMENIRRNARHGMPQFGGINNDDLRFKTWTEDVRYETNAVIIAMRDVSG
                     SMGEFEKYISRSFYFWMVKFLRQKYNQVQIVFITHHTEAKEVDEQTFFTLGESGGTKV
                     SSAYRLALDIIHARYNPQDWNIYPFHFSDGDNWGETDNRLCVDLVHEMLDRCNVFGYG
                     EIRQGGYGSLSTLMSAFQNIKDDKFIGVTIEDRAGVYPALRRFFTEQTGVGA"
     gene            1598..2914
                     /locus_tag="VHS06_09660"
     CDS             1598..2914
                     /locus_tag="VHS06_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX2988425.1"
                     /translation="MNSNTVISSNKEMRELERAIEEIWEIALRLGLDPFPTHFEMVPA
                     AIMYEFGAYGIPGRYSHWTHGKAYQRMKTMYDYGLSKIYELVINTNPCYAFLMEGNSQ
                     LQNKLVAAHVLAHGDFFKNNLHFRHTSRQMLEGASINAERIRGYEFHHGRNEVEEFLD
                     RVLCIEEHVDPNLSLRPKEQRSEPEEHKSAPAETPYDDLFSLEAEPEPNTEEPPRRHK
                     VPAEPEKDILLFVAEHSADLEEWQRDIIQIVRNEQLYFVPQMMTKVMNEGWASYWHRE
                     IMHELALTEDEYVQFANLNAGVLAPSPHQINPYHVGLKIWESIKARWDNPTEAERREF
                     GRNGGEGLAKMFEVREVDSDVSFLRNYLTRDLVDELDLYIYKREGDQWVVVEKDWERI
                     RDMLVASMTNFGVPYIVVEDGDYHHNRELYLRHSFEGQELDLKYAE"
     gene            2937..3239
                     /locus_tag="VHS06_09665"
                     /pseudo
     CDS             2937..3239
                     /locus_tag="VHS06_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
BASE COUNT          794 a          978 c         1088 g          625 t
ORIGIN      
        1 cggtgttacg gagaacgcca agaaggcatg ccgtgaggag atcctgatcc gcatcagcgc
       61 tctggcgcgc cgtgggcagc ggctggatta ccgcagccac gagcggctga aggaggcgat
      121 cgagaagaag ctgttcgccg accttcgtga cgtggtcaag attaccacgt ctaccaagac
      181 gccggatgcg gagcagttga agcggatcaa cgatgtggtc gacaggctgg tgaaggagca
      241 gggttactgc cacgtctgtg cgaacgatct cctcaactac gtgggcacgc tgctgaaccg
      301 ttagtgcgga gtactagagt ctagagaacg agagtcgagg atcgaggatc gagaggctta
      361 gggagtcatg gactctcgac tcttggctct cgactctcga ctctcggctt aaaggagact
      421 ctcctcaggg ggagtaaaca atgggcaatt tcaccatatc cctctcgaag cacgactggt
      481 cgttgcaccg caaggcgcca caggaccagg cgcgccacaa cgagaaggtt cgagaagcca
      541 taaagagcaa cctcgcggat atcgtcgcgg aggaggcaat tgtcacctcg gacgggaaca
      601 gggcgatcaa ggttccgatc cgctcccttg agctccccca cttccgctat gacacgaacc
      661 gaaaggacca cctgggccag gggaacggag actcccaagt tggtgacgtg ctcgggcagg
      721 aagccggcca ggatggcccc ggcaatggga agggcgcggg cgaccagccg ggcctcgact
      781 actacgaggc cgagattacc atcgacgagt tggccgacct gctcttcgag gacatggggc
      841 taccgaatct agagcagaag caggctcaac agatcgagag taccataatc cggttcaacg
      901 acatccggaa gagcgggcca atgtcgagtc tggacaagcg acggacggtg atggagaaca
      961 tccgtcgaaa cgccaggcac ggaatgccgc aattcggagg gatcaacaac gacgacctcc
     1021 gcttcaagac ctggaccgag gatgtccggt acgagacgaa cgcggtgatt atcgccatgc
     1081 gcgacgtctc cggatccatg ggcgagttcg agaagtacat cagcaggagc ttttacttct
     1141 ggatggtgaa gttcctgcga cagaagtaca accaggtgca gatcgtcttc atcacccatc
     1201 ataccgaggc caaagaggtg gatgaacaga ccttctttac actcggcgag agcggtggca
     1261 ccaaggtatc ctcggcctat aggctggcgc tcgacataat tcacgctcga tacaacccgc
     1321 aggactggaa catctacccg ttccacttct cagacgggga caactgggga gagaccgaca
     1381 atcgcctctg tgtcgacctg gtgcacgaga tgctggacag gtgcaacgtg ttcggttacg
     1441 gcgagatcag gcaagggggc tatggctccc tcagcactct gatgtccgct ttccagaaca
     1501 tcaaggacga caagttcatc ggggtaacca tcgaagacag ggcaggggtc tatccggccc
     1561 tccgcagatt cttcaccgag cagaccggag taggggcgtg aacagcaaca cggtgatcag
     1621 cagcaacaag gagatgcggg agctggagcg ggcgatcgaa gagatctggg agatcgcatt
     1681 gaggctgggg ctcgacccgt tcccgaccca tttcgaaatg gtgccggcgg ccatcatgta
     1741 cgagttcggc gcctacggca tcccgggacg ctattcccac tggacgcatg ggaaagcgta
     1801 ccagcggatg aagacgatgt acgactacgg cctgagcaag atctatgagt tggtgatcaa
     1861 caccaatccg tgctacgcct tcttgatgga gggcaacagc cagttgcaga acaagctggt
     1921 agctgcgcac gtgttggccc acggcgactt cttcaagaac aatcttcact tccgccatac
     1981 ctcccgccag atgctggagg gtgccagcat caacgcggag cggatacgcg ggtacgagtt
     2041 ccaccacggt cgcaacgaag tggaggagtt cctggacagg gttctgtgca tcgaggagca
     2101 tgtggaccct aacctctcac tgagaccaaa ggagcagagg agcgagccgg aggagcataa
     2161 gtctgctcct gcagagacgc cctacgacga cctgttcagc ctggaggcgg agccggagcc
     2221 gaataccgag gagcctccac gcagacacaa agtgcctgcc gagccggaga aggacattct
     2281 gctcttcgtc gccgagcact cggcggatct ggaggaatgg cagcgtgaca tcatccagat
     2341 cgtgaggaat gaacagctct acttcgtccc gcagatgatg accaaggtga tgaacgaggg
     2401 atgggccagc tactggcaca gggaaatcat gcacgagctg gcgcttacgg aggatgagta
     2461 cgttcagttt gccaacctca acgcgggggt cctggctcca tcgcctcacc agatcaatcc
     2521 ctaccatgtc ggtttgaaga tctgggagtc gataaaggct cgttgggaca accctacgga
     2581 ggccgagcgt cgggagttcg gccgcaacgg cggcgaaggt ctggccaaga tgttcgaggt
     2641 cagggaagtg gacagcgacg tgtccttcct tcgcaactac ctgactcgcg atctggtgga
     2701 cgaactggac ctttacatat acaagcgaga gggcgaccag tgggtcgtgg tggagaagga
     2761 ctgggagagg attcgggaca tgctggtggc cagcatgacc aacttcggcg ttccgtacat
     2821 cgtggtggag gacggggatt accatcacaa tcgggagctg tacctccgcc actccttcga
     2881 gggccaggag ctggatctga agtacgcaga atgaccccta ctgggctgcc gctcaggcgc
     2941 tggcggctga gatggggccc gctgcccagg caaccgtcga ggtaggcttc aacgaattct
     3001 gcaaccccac cctggatgat gcgctggata acgcgatgac ggcaagtccg gatagggtgg
     3061 tcgtggttac ccctatgatg actcggggcg gaggccattc cgagatagac atcccacagg
     3121 ccatcggtcg agcgaaggag cggcactcgg gcgtgccgat agtgtacgcg tggcccttcc
     3181 ccgtgtcctc catcgcggag tttctggcca cgcagatcca gaagttcgaa atacaataga
     3241 acgcgacgcg cgtcctatta gtaggggcgg cgcaaccgtg aagctgcgct cgctcgcttt
     3301 gcgggggcgc cccgccctac cattctccct ccccgtgtct ccgggtctcc ccgtctccgt
     3361 gtcccctacg gataggtctg ggtgtggtgc tctccgtcga atgagagcag tagcttccga
     3421 tcctcggcca ccgtctccag gtgtaccggc ctgttccaga gatcgtacac cgcctgcatc
     3481 gtctt
//
LOCUS       DATALS010000437         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_436142, whole genome shotgun sequence.
ACCESSION   DATALS010000437 DATALS010000000
VERSION     DATALS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436142"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VHS06_09670"
     CDS             complement(<1..530)
                     /locus_tag="VHS06_09670"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEX2988426.1"
                     /translation="MSKLETIERPAVSHDSLSGELLSVMRELERLCQQLLEVTGLERR
                     LVLDGRIGDLDDTTRKKNELMERMDRIEVLRRDLSARTAVEYGLPQDASLARIATRME
                     KQAAADLLELRQRVSDGISRLKDLNETNQMLVRKSLDMVRNSIRQLSRSVGAGQSYTP
                     NGYPAMIRLGNGMVDRR"
     gene            complement(527..820)
                     /gene="flgM"
                     /locus_tag="VHS06_09675"
     CDS             complement(527..820)
                     /gene="flgM"
                     /locus_tag="VHS06_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEX2988427.1"
                     /translation="MDISSNGMGPIRDFIKTNSMDQSYRSMQKGRSQTTPSLDSVTIS
                     DRGLDLARIKDAVLSAPDVREEKVAATQEAVAQGSYNMSPVEIAKAILASRMR"
     gene            complement(908..1690)
                     /locus_tag="VHS06_09680"
     CDS             complement(908..1690)
                     /locus_tag="VHS06_09680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HEX2988428.1"
                     /translation="MIRGIQTVVSAMQAQQKRQEIMAKNLSNASTTGYKQECTSVEGF
                     AATIGRLEKGGSRSGLALTATSPSLGSLGADLGVDRSWVDFRQGGLEQTQRKLDVALN
                     GDGFLQVRTPDGDFYFRGGALQVDSDGRLVTEEGYSVLADDGQEIYLPDGDPTIKADG
                     SIFVGDEPVASLSLVEFDPGTPMAKVGECLYSPDDPNSQPLAPIATTVKQGFLEQSNV
                     DQISVMTEMVTLLRAYQASQRMILAQDELLGKAVNEVGKIQG"
     gene            complement(1748..2446)
                     /locus_tag="VHS06_09685"
                     /pseudo
     CDS             complement(1748..2446)
                     /locus_tag="VHS06_09685"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
BASE COUNT          475 a          859 c          666 g          576 t
ORIGIN      
        1 cgtcgatcta ccatgccgtt gcccaggcgg atcatcgcag gatagccatt cggggtgtag
       61 ctctggcctg ccccaacgga tcggctcagt tgccgaatgg aatttctaac catgtccaac
      121 gacttccgca cgagcatctg attggtctca ttcagatcct tcagcctgga gatgccgtcg
      181 gagacacgct gcctcagttc cagtagatcc gctgctgcct gcttctccat ccttgtggcg
      241 atgcgtgcaa gggacgcatc ctgcgggagg ccatactcga ccgcagttcg agccgacaga
      301 tcacgtctca gtacctcgat ccgatccatc cgctccatca gctcgttctt cttccgtgta
      361 gtatcgtcca gatcaccgat ccgcccatcg agcaccaatc ttcgctcgag acccgtaacc
      421 tctagaagtt gctgacaaag acgctctagc tctcgcatca ccgacagcaa ttcgccgctc
      481 aggctatcat gggatacggc cgggcgctcg attgtctcca gtttgctcat ctcatcctcg
      541 acgccaggat cgccttggct atctcgacgg gcgacatgtt gtaactcccc tgcgcaacgg
      601 cctcttgcgt cgcagccacc ttctcctccc gcacatccgg cgcgctcagc accgcgtcct
      661 tgatccttgc cagatcgagc ccacggtccg agatagtcac cgagtcaagg cttggagtag
      721 tctggctccg gcccttctgc atcgaccgat acgattggtc catcgagttc gtcttgatga
      781 agtctcgaat cgggcccatg ccgttgctgg aaatgtccat tcttgctcac ccccagagct
      841 ctgcctaagc gatctgactt tacacgtcac tagtaattat cggcactttc cgtcaggact
      901 ttagccttta gccctggatt ttgccgacct cgttcacggc ctttccgagc aactcgtcct
      961 gcgcaagaat catcctctgg ctcgcctgat acgcacgcag cagcgtcacc atttcggtca
     1021 tgactgagat ctggtctacg ttggactgct ccaggaaacc ctgtttcacc gtggtagcga
     1081 tcggggccaa cggctgcgag ttggggtcgt cgggtgaata gagacactcc ccaactttcg
     1141 ccatcggagt gcccggatcg aactcgacca gggatagcga cgccactggc tcgtcgccta
     1201 caaagatgct cccgtccgcc ttgatggtcg ggtcgccatc cggcagatag atctcctgac
     1261 catcgtccgc cagcaccgag taaccttcct cggtcacaag tctgccatcc gaatctacct
     1321 gcagtgcgcc gccccggaag tagaagtcgc cgtcgggcgt tcgaacctgg aggaaaccat
     1381 cgccattgag ggcgacgtcc agctttcgct gcgtttgctc caacccaccc tgccggaaat
     1441 ccacccagga tcggtccacc ccaaggtcgg cccccaggct tcccaaagag ggtgaggtcg
     1501 cagtgagtgc aagcccggaa cgtgaaccgc ccttctccaa ccggccgatg gtggccgcaa
     1561 acccctccac actggtgcac tcctgcttgt agccggtcgt cgacgcgttg ctcaggttct
     1621 tggccataat ctcttgccgc ttctgctgag cctgcatcgc cgacaccacg gtctggatgc
     1681 cgcggatcat tggaacctcc ttgaagctag gccgcttccc tgtccggagc gctagtgttc
     1741 gacggcgcta gtcctgctgg cgccagagtt gaagctgcgc cttaagcctg agcaccgccc
     1801 tggtgtggag ctggcacacc cgcgattcgg aaacgtccat gacacgactt atctctcgca
     1861 tcgtcagctc ttccttgtag tagagggcca gtagaagcct ttcgcggtcg cttagtctgg
     1921 aaattgcctc cgccagagcg tccagcagac cccgccgctc cgccagcgac acgggatccg
     1981 ggctgttggt gtcctccagc gtcgacagta gggtcgcgac ttcgctctcc ccgtctcccc
     2041 ccatcaccga atcgatggag atggtcgccg ggccgacttc agtcagcacc tttctgtagg
     2101 cattgatgtc catcccaagg tgctcggcca cctccctatc attggggagt ctgcccagcg
     2161 tgatctgcag agcggtaatc gccttctcga tttcgcgtgc cttctggcga acggatctag
     2221 aaatgacgtc cagcgaccgc aactgatcga gaatcgcacc tttgatgcag gtcactgcgt
     2281 agttctcgaa tggcacgccc ctcgtgggat cgtagtgctc cactgcgcgt atcaaaccga
     2341 ccatcccgga actgagcaca tcgtccatgt ccaggacagg aggtagggcg atggagagcc
     2401 ttcccgtcac atacttcacc aaaggtgcgt actgcaggat tatgctctcg cgtgcagttc
     2461 ggtcgccccg tccgctatgt gtttgtcatt ggtcctctat gactgctgct gctgccactc
     2521 cgccatcacc tcctactcat cccacccact ggtacccaag gccccactat tcggag
//
LOCUS       DATALS010000438         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_345430, whole genome shotgun sequence.
ACCESSION   DATALS010000438 DATALS010000000
VERSION     DATALS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345430"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1238
                     /locus_tag="VHS06_09690"
     CDS             123..1238
                     /locus_tag="VHS06_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX2988429.1"
                     /translation="MEPRRALGQVGESVLRRRAERLFLVTDVGVTQAGWVDRAIPYLE
                     EQGLPWVLWKGATPNPKDLEVEQGAREYIAANCDAILAIGGGSVIDAAKAIAVLVTNG
                     GRIHDYEGIGKITHPLPPMVMVPTTAGSGADVSQFAIITDTTRRVKMVLGSKSLIPDI
                     SITDPLLLTTKDPALTAATGMDALTHAVEAYVSIAATPLTDVFALSAIRLVARELRAS
                     VASRGNLEAKRGMAMASLQAGAAFSNAILGATHAMAHQLGGMLDLPHGVVDAILLPQV
                     MEFNLISSVGRYADIAAALGESTANLSQPEAANRAIKAVRDLARDIGIPSGLAAVGVD
                     AADIPALSENSMHDVCLATNPRDTGVSDIAELFRRSL"
     gene            1264..2778
                     /locus_tag="VHS06_09695"
     CDS             1264..2778
                     /locus_tag="VHS06_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX2988430.1"
                     /translation="MGLEDRSDLLEKLAGLKIQKGMPHYPEEKDAALAALTGINSSKL
                     GYYRELKGKVHELEASNEQLREAKRVADDLYEQVRRNAEELSSLYERSLLQAKRLDLA
                     IESLKSISQVLTTTTRGVEVLLQSVVRTAAALFDTEYAVMTVGVPGGAEQVAYCAYGL
                     PSSAFPAIAPRRLLDLIDVAARERRPVTDPPGAPCDRPVTLCVPMFRDDLLVGNLCLQ
                     FREGRDIAESDLSILQTLASQAAVAVENARLFEESQRLQLSTRQLYEMAQEQKDRAEK
                     RTQELTLARDELTLMQQEQLLNEERNRIARELHDSVAQILISIGLNLEWCRQQVGEES
                     PAHEKLLSLKELARSGVNEIRSSIFRLWSIEILDIGMIPALERMTREFQRITGIEVEL
                     ATCGEVVRLPLHVETAIYRVAQEACNNVFKHAHASRVRLEVAYQPWSVVLQVTDDGVG
                     IANTDLSHHRQGATYGVKIMLARVEQIGGSLEIHNLSSNRGGTRVIARVPLKEM"
     gene            2780..>3132
                     /locus_tag="VHS06_09700"
     CDS             2780..>3132
                     /locus_tag="VHS06_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2988431.1"
                     /translation="MGVARILLVDDHCIVRQGIKALLQLERDMTVVGEASDGATALAL
                     IDELKPDLVLLDAKMSDMDGVEVCRQAMARHPNQSIVILTAFPSQDSVLQCIRAGAKG
                     YVLKDVDVTELVRTVR"
BASE COUNT          658 a          880 c         1022 g          572 t
ORIGIN      
        1 ggactcacca tccaacagca cgaaggcaag ggcgacgacc gagagcccaa ttcccgctat
       61 ctcgagggca ttgaagatga gcgacatggg ctgcccgaag agaagggagg caaacaccag
      121 cactggagcc acgaagagcg cttggacagg tcggtgagag cgttcttcgc cgccgcgccg
      181 aacggttgtt tctggtcacc gatgtggggg tgactcaggc gggttgggtg gatagggcca
      241 taccgtacct ggaggaacag ggcctaccct gggttttgtg gaaaggggcg acgcccaacc
      301 ccaaagacct ggaggtggaa cagggggcgc gagagtacat cgccgccaat tgcgatgcta
      361 tccttgccat cggcggtggg agtgtcatcg acgctgccaa ggcgattgcg gtactggtaa
      421 ccaacggggg gcgcatccac gactatgagg gaatcggcaa gatcacccac ccccttcctc
      481 ccatggtcat ggttcccacc actgcgggta gcggcgcaga cgtgtctcag ttcgccatta
      541 tcaccgacac cacgcgcagg gtgaagatgg tgctgggcag caaatccctg atcccagata
      601 tctccattac cgatcccctc cttctcacca ccaaagatcc tgccctaacg gcagccacgg
      661 gcatggatgc cctgactcac gcggtggagg catacgtctc catcgccgcg acccccttga
      721 ccgacgtatt tgcccttagc gctatccgtc tggttgccag ggagttgagg gcgtccgtgg
      781 ccagccgggg caacctggag gcgaagcgtg gcatggcgat ggctagcctg caggccggcg
      841 cggctttctc caacgccatc ctcggcgcca cccatgccat ggcccatcag ttgggaggga
      901 tgctcgacct gccgcacggc gtggtagacg ccatactgtt gccccaagtg atggagttta
      961 acctgatctc ctcagtcggg cgctacgcgg atattgccgc agctctaggg gagagcaccg
     1021 ccaacctgag ccaaccggag gccgcaaaca gggctatcaa ggcggtgcgg gatctggcga
     1081 gggacatcgg tatcccttcg ggtctggcgg ccgttggagt ggacgcggca gacatacccg
     1141 ccctgagcga gaactccatg cacgatgtct gtcttgccac caatcccaga gacactggtg
     1201 tttccgacat agctgaactg ttcagaagat cgctgtgacc gcgatgatgg agcggctcga
     1261 ccgatgggac tcgaggacag aagcgacctg ctggagaaac tcgcggggct gaagatccag
     1321 aaggggatgc cccactaccc ggaggagaag gatgcggcgc tggctgcgct gaccggtatc
     1381 aactcctcca agcttggcta ctaccgagag ctgaagggga aggttcacga actcgaggcc
     1441 agtaacgagc agctgcgaga ggcgaagcgg gtcgccgacg atctgtacga gcaggtgcgc
     1501 cggaatgcgg aggagctgtc ctcgctgtac gagcggtcgt tgttgcaggc gaagagactg
     1561 gatctggcca tagaatcgct gaagagcatc tctcaggttc tgacgacgac gacccgcggg
     1621 gtagaggtac tgttgcagtc tgtcgttcgg actgctgccg cgctgtttga cacggaatat
     1681 gccgtgatga ccgtcggcgt gcccggaggg gctgagcagg ttgcgtactg cgcgtacggg
     1741 cttccctcct cggccttccc ggccatagcc cccaggaggc tgctggacct catagacgtt
     1801 gccgctcgtg agcgccgccc ggtcaccgat cctcccgggg cgccctgcga tcggccggtg
     1861 acgctatgtg tgccgatgtt ccgcgacgat ctgttggtgg gcaacctctg ccttcagttt
     1921 cgggagggac gcgatattgc cgagagcgac ctttccatcc ttcagaccct cgcaagccag
     1981 gcggcggtgg ccgttgagaa tgcgcggttg ttcgaggaga gtcaacggct gcagttgagc
     2041 acccgacagt tgtacgagat ggcccaggag cagaaggacc gcgcggaaaa gagaacgcaa
     2101 gagctgaccc tggccaggga tgaactcact ctcatgcagc aggaacagct tctcaacgaa
     2161 gagcggaacc gcatcgcccg tgaactgcac gacagcgtag ctcagatact gataagcatc
     2221 ggcctcaatc tggaatggtg ccgccagcag gtgggggagg aatcccccgc ccacgaaaag
     2281 ctgttgtcgc taaaggaact cgcgcgcagc ggagtgaacg aaataaggag cagcatcttc
     2341 cggctgtggt ccatcgagat cctcgacatc ggcatgatcc ccgcgctgga gaggatgacc
     2401 agggagttcc agaggatcac gggcatcgag gtggagttgg cgacatgcgg agaggtagtc
     2461 agactgcctc tccacgtgga gacagccatc tatcgagtgg cccaggaggc atgcaacaac
     2521 gtattcaagc atgctcatgc cagtcgagtg agactggagg tggcctatca gccatggtcc
     2581 gtcgttctgc aggtgacaga cgacggagtg ggtatagcga acaccgacct cagtcatcat
     2641 aggcaggggg ctacctatgg tgtgaagata atgttggctc gtgtggagca gatagggggt
     2701 tcactggaga tccacaatct ctcgtcgaac aggggcggaa cgagggtgat tgcccgcgta
     2761 ccgctaaagg agatgtgaga tgggagtagc acgcattctt ctggttgacg atcactgcat
     2821 cgttcgacag gggatcaagg ccctccttca gttggagcgg gatatgacgg tggttgggga
     2881 ggcgagcgac ggtgcgacgg ccctggcgct tatcgacgag ttgaagccgg acctggttct
     2941 cctggatgcc aagatgtccg atatggatgg cgttgaagtg tgtcgccagg caatggcccg
     3001 gcaccccaat caatccatag tcattctcac cgcgtttcct agccaggata gtgtgctcca
     3061 gtgcatccgc gccggagcta agggctacgt tctgaaggat gtggacgtca ccgagttggt
     3121 gaggaccgtt cg
//
LOCUS       DATALS010000439         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_297926, whole genome shotgun sequence.
ACCESSION   DATALS010000439 DATALS010000000
VERSION     DATALS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297926"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VHS06_09705"
     CDS             <1..1027
                     /locus_tag="VHS06_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2988432.1"
                     /translation="NLFLQVGDLFDTPDPRNLERQFVAEQLAGLRDAGIACIGIGGNH
                     DTPRSRGDRPAATPMSPYASLGGLRLLEGQGTVDSELLELEGMRVAVGGLEPDPTAQP
                     GSDPLDGVDWAPDADFSLLLVHGSPEGHIYPDAPEAILRRQTVDRLHGIDCILAGHVH
                     RHAVFHWGSTTVIVPGATERMTFADLEERPGFVYMELERHHIARIDHLPVQAQPRRTL
                     LIPTSELADSGIAEQVMGMMEGVCAPDALVRVTLAGPITRQRYHDLHLRDLAEFGAAR
                     CFFLDLDTTGLFLEDEAKLTIFRGGRLSQREELIRFARESRDAATTDEERSLVDDALI
                     AILEEYS"
     gene            1024..>2318
                     /locus_tag="VHS06_09710"
     CDS             1024..>2318
                     /locus_tag="VHS06_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2988433.1"
                     /translation="MILLKRLQVENFKLLRRIDLTFQRQGAVLVEGLNESGKSTLFES
                     VFYGLYGLPLVTEGRGRGNLESVIRYGADSMKVVLSVEVEDAELEIARSLKRGRSSQA
                     SLTVRRPGLPDEAVSGVQAVNARIVDELGGLDGDALLNSCFVEQKKLSKLEDLAPAQR
                     KESLRRLLNLERLTELAAAFRITAQDERELADARDRLELARLSERLPGLGQIRAELDA
                     SSAGMAVAGVLSSEERESLQFEAADYFQDAPPTEGAAPWKRYSGALKAARAAEARARK
                     RGLLLAGAAVLSLGVVAAFVVAGVSAALLGVAVSAVAALLSVRAYRSAESAAAEATAQ
                     AARLGIWRDMQLATAGELEGRRRRLEERVGVDGSNLVAEEWRREVERLSRRMQVRRKA
                     AALVEGAMERIVRMVLPNTERNLGQILPLLTEGSYHEAR"
BASE COUNT          373 a          717 c          843 g          385 t
ORIGIN      
        1 caacctcttc ctgcaggtgg gagacctctt cgataccccc gatccccgca atctggagcg
       61 ccagttcgtc gccgagcagc ttgctggact acgagacgcg gggatcgcct gcatcggcat
      121 aggtggcaac cacgataccc cccgttcccg gggtgacaga cccgcggcaa cacccatgtc
      181 cccatacgcg agcctgggcg gccttcggct gctggagggg cagggcaccg tggatagcga
      241 gctgctggag ctggagggga tgcgagtggc cgtgggggga ctggagcccg atcccaccgc
      301 tcagcctggc agcgatccac tggacggggt ggactgggcg ccggatgccg acttttccct
      361 gctgctggtc cacggcagtc ccgaggggca catctacccc gacgctccgg aggcgatcct
      421 gcgccggcag acggtggata gactgcacgg catcgactgc atcctggcgg ggcacgtcca
      481 ccgccacgct gtcttccact ggggaagtac aaccgttatc gtgcccggcg ccaccgagcg
      541 gatgaccttc gccgacctgg aggagcggcc cggcttcgtc tacatggagc tggagcggca
      601 ccacatcgcc aggatcgacc atctgccggt tcaggcgcag ccgagacgca ccctcctgat
      661 ccccacctcg gaactggccg attccggcat cgccgagcag gtaatgggga tgatggaggg
      721 tgtgtgtgcg ccggatgccc tcgttcgagt cacgctggcc ggacccatca cccggcagcg
      781 ctaccacgat ctgcacctac gggacttggc cgagttcggc gcagctcgct gcttcttcct
      841 ggatctggat accaccgggc tcttcctgga ggacgaggcg aagctgacca tcttccgcgg
      901 cggccgcctc tcccagcgcg aggagttgat ccgctttgct cgcgaaagcc gcgacgccgc
      961 taccaccgac gaagagcgct ccctcgtcga cgacgccctc atcgcgatcc tggaggagta
     1021 ctcgtgatac tgctgaagcg gctgcaggtg gagaacttca agctgctgcg gcggatagat
     1081 ctgaccttcc agcggcaggg ggcggtgttg gtggaggggc ttaacgagtc cggcaagtct
     1141 accctcttcg agagcgtctt ctatggcctc tacggtctgc ctctggtcac cgagggccgg
     1201 ggcaggggga acctggagag cgtcatacgc tacggagccg attccatgaa ggtggtgctc
     1261 tccgtcgagg tggaagacgc cgagctggag atcgcgcgct ctctgaagcg gggccggagc
     1321 agccaggcga gtctcacggt gcggcggccg ggcctgcccg atgaggccgt ctccggtgtt
     1381 caggcggtga atgcgcggat agtggacgag ctgggggggc tggatggcga tgccctgctg
     1441 aattcctgct tcgtggagca gaagaagctc tccaagctgg aagacctggc cccggcgcaa
     1501 cgcaaggagt cgctgcgccg gctgctgaac ctggaaaggc tgaccgaact ggcggcagcc
     1561 tttcgcataa cggcgcagga cgagcgggag ctggcggacg cgcgggatcg gctggagctg
     1621 gcgaggctgt cggaacgtct cccggggctg ggccagatca gggctgagct ggatgcctct
     1681 tcggccggta tggccgtggc cggcgtcctc tcgtccgagg agagggaatc cctccagttt
     1741 gaggcggcag actacttcca ggatgctccg cccacggagg gtgccgcgcc ctggaagcgc
     1801 tactccgggg cgctgaaggc cgccagagct gccgaggccc gagcccggaa acgagggctg
     1861 ctgctcgcag gcgccgctgt gctatccctt ggcgtggtgg ctgccttcgt cgtggcaggc
     1921 gtctccgccg cattgctcgg ggtcgccgtc tcggcggtcg cggcgttgct ttcggtccgc
     1981 gcctatcgct cggcggagag cgcggccgct gaggccactg ctcaggccgc ccggctcggc
     2041 atctggagag acatgcagct tgcgacggcc ggggagctgg aggggcgaag gagacggctg
     2101 gaggagcggg tcggtgtgga cggctccaat ctggtggcgg aggagtggcg ccgggaggtg
     2161 gaacggctct cccgaaggat gcaggtgcgg cggaaggcgg cggctctggt ggaaggggcc
     2221 atggagagga tagtccggat ggtgcttccg aacacggagc ggaatctggg gcagatcctg
     2281 ccgttgctga ccgaggggag ctatcacgag gcacgtat
//
LOCUS       DATALS010000440         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_298668, whole genome shotgun sequence.
ACCESSION   DATALS010000440 DATALS010000000
VERSION     DATALS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298668"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1846)
                     /locus_tag="VHS06_09715"
     CDS             complement(<1..>1846)
                     /locus_tag="VHS06_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988434.1"
                     /translation="GNRRNLLIVMLVLLLCAVRGEAAVIDSKSDTWVATDAFGRTLPG
                     YKECGPQRSGKFVGIFYFLWLGEHGTGGPYDISKLLAANPSNPAWGPVNAFHHWGESE
                     LGYYLSNDSYVVRRHCQELVDAGVDVIIFDATNGFTYTDNYMKLCSVYSQIRSEGGNT
                     PQIAFMAHSSSDAVVTQLYNDFYSQNLYPELWFRWLGKPVILSPKGNLPQNIRDFFTF
                     RNSWAWSTGQDQWPWLDNYPQTPSWHVSGVPEEISVCVAQHPVSNIGRSFHGGAEPPV
                     GSQPTEQGLCFAEQWRRALEVDPEFIFITGWNEWVAQRFLSDGNQTFLGQPLPSGGTF
                     FVDEYNQEYSRDIEPMKGGHTDNYYYQMIDGIRRFKGVRAPEAASAPKTITIDGGFSD
                     WTDVQPEYRDTMYDTTARNAAGWGSAGTYVNTTGRNDFVKLKVAYDATYVYFYAETRA
                     NMTSYTGANWMMLFIDSDRDPSTGWQGYDYLVNWPPSSSSSTSLKRNAGGWNWTSVRS
                     TAYRYTGNKMEIRVLRSDIGQAGTPQVNLDFHWVDNIQKTGDITEFFVSGDSAPDRRF
                     SYRYSPPPASVTNLVAAGGDGQITLSRTNPSDADFAGTVVRCKAAGYPG"
BASE COUNT          316 a          552 c          580 g          398 t
ORIGIN      
        1 tcccggggta gcctgcggcc ttgcaacgga ccaccgtccc ggcgaaatcg gcatcggacg
       61 ggttggtcct ggagagtgtt atctgcccgt ccccgcccgc tgcgacgagg ttggtgacgg
      121 atgccggcgg cgggctgtag cggtagctga aacgtcggtc cggggcgctg tccccgctta
      181 cgaagaactc ggtgatgtcc cccgtcttct ggatgttgtc gacccagtgg aagtccaagt
      241 tcacctgcgg cgtccccgcc tggccgatgt cggagcgcag cacccttatc tccatcttgt
      301 tcccagtgta cctgtaggcg gtgcttcgca cggaggtcca gttccagcct ccggcatttc
      361 tcttgaggct tgttgaggaa ctcgagcttg ggggccagtt gaccaggtag tcatagccct
      421 gccagccggt tgaagggtcg cggtcggagt ctatgaaaag catcatccag ttcgcgccgg
      481 tgtagctcgt catgttggcc ctggtctcgg cgtagaagta gacgtatgtg gcgtcgtagg
      541 cgaccttgag cttgacgaag tcgtttcgcc cggtggtatt cacgtaggtc ccggcgctcc
      601 cccaccctgc ggcgtttctg gcggtggtgt cgtacatcgt gtcgcggtat tcgggctgga
      661 catcggtcca gtcgctgaaa cccccgtcta tggttatcgt cttgggcgcg cttgcagctt
      721 ccggtgcgcg aacccctttg aacctgcgga tgccgtcgat catctgatag tagtagttgt
      781 ccgtgtgccc gcccttcata ggctcgatgt cgcggctgta ctcctggttg tactcgtcca
      841 cgaagaaggt ccctcccgaa ggaagcggtt gcccgagaaa ggtctggttc ccgtcgctca
      901 ggaagcgctg cgcgacccat tcgttccaac cggtgatgaa gatgaactcc gggtcgacct
      961 ccagggcacg ccgccactgc tcggcaaagc acagcccctg ctccgtcggc tggctgccaa
     1021 caggcggctc cgccccgccg tggaagctcc ttccgatgtt ggataccggg tgctgggcca
     1081 cgcagacgga tatctcctcg ggaacccctg agacgtgcca actcggggtc tgcgggtagt
     1141 tgtccagcca gggccactgg tcctgccctg tactccaggc ccaggagttg cggaaggtga
     1201 agaagtcgcg tatgttctgc ggcaggttgc ccttcggcga gagtatcacc ggcttgccca
     1261 gccacctgaa ccagagctcg gggtacaggt tctgggaata gaagtcgttg tacagctgag
     1321 tgacaacggc atcgcttgac gagtgggcca tgaaggctat ctgcggcgtg ttccctcctt
     1381 cgctccttat ctgcgagtag acgctgcaga gtttcatgta gttgtccgta taggtgaagc
     1441 cgttggtggc atcgaagatg atgacatcca ccccagcatc gaccaactct tggcagtgtc
     1501 tgcggacgac gtaggaatcg ttggagagat agtagcccag ttccgactcc ccccagtggt
     1561 gaaaggcgtt caccggcccc caggcagggt tgctggggtt tgccgcaaga agcttgctga
     1621 tatcgtaagg cccgccggtg ccgtgctccc cgagccacag gaagtagaat atgccgacga
     1681 acttgcccga acgctgaggg ccgcactctt tatagccggg cagcgtgcgc ccgaacgcgt
     1741 ccgttgcgac ccaggtgtcg ctcttgctgt cgatgacggc cgcttcgccc cgaaccgcac
     1801 aaagcagaag aacgagcata acgatgagga ggttacgtct gtttcc
//
LOCUS       DATALS010000441         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_299100, whole genome shotgun sequence.
ACCESSION   DATALS010000441 DATALS010000000
VERSION     DATALS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299100"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VHS06_09720"
     CDS             <1..244
                     /locus_tag="VHS06_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX2988435.1"
                     /translation="VELAGAVAHEMNQPLTVIISGAELMAHPGRSVEEVREVASRMAE
                     ASERMADIVVKLQKATCYRNKPYVGKVRIVDLDRAG"
     gene            complement(241..849)
                     /gene="metW"
                     /locus_tag="VHS06_09725"
     CDS             complement(241..849)
                     /gene="metW"
                     /locus_tag="VHS06_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX2988436.1"
                     /translation="MEIPVLSDRFDLKLICNLVEPGSSVLDLGCGDGDLMAMLVNERG
                     VTARGVEISEDGVYRSIARGLSVHHGDLDEGIEDYPDSSFDYVILSQTLQAVHKPRLV
                     LQEMLRVGKIGIVSFPNFAFWQARLNLALQGRMPKTDDLPYEWYNTPNIHLTTIQDFL
                     DLCQTEGMEVVKRVFLSGNHHVRLFPNVRARTALFMVRKKNL"
     gene            complement(876..>1813)
                     /locus_tag="VHS06_09730"
     CDS             complement(876..>1813)
                     /locus_tag="VHS06_09730"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX2988437.1"
                     /translation="VDRKRLGWWDNMIGPGKAIDTNHYFVICSNVLGGCRGSTGPSSI
                     NPSTDKPYGLSFPVVTVGDMVRAQKALVDHLGIGRLLAVIGGSLGGMQALQWAAAYPD
                     RVKACIPIATAARLSAQGIAFNEVGRQAIMADQHWHNGDYYGKNSPIPGLAVARMLGH
                     ITYLSDESMRQKFGRRLRNKETYGFDFSTDFEVESYLRHQGDAFIHRFDANSYLYITK
                     AVDYFDLSNGTGALGAAFRDVQARFLVISFSSDWLYPTYHAKEIVKALKLNGIDVSFC
                     EIPSAYGHDAFLLEEKAQTHLIRGFLSYTMSGKVR"
BASE COUNT          344 a          539 c          561 g          369 t
ORIGIN      
        1 cgtagagctg gcgggagccg ttgcccacga gatgaaccag cccctcaccg tcatcatcag
       61 cggcgcggag ttgatggctc acccagggcg aagtgtcgag gaagttcgag aggttgcgtc
      121 gcgcatggcc gaggcatccg agcgcatggc cgatatcgtg gtgaagctgc agaaggcaac
      181 ctgctatcgc aacaaaccgt acgtcggcaa ggtgcgtatc gtcgacctcg atagggccgg
      241 ttagaggttc ttcttgcgca ccatgaagag ggctgtgcgg gccctcacgt tgggaaacag
      301 ccgcacgtgg tggtttcccg agaggaagac cctcttcacc acttccatgc cctcggtctg
      361 gcagaggtcc aggaaatcct ggatcgtcgt cagatggatg ttgggagtgt tgtaccactc
      421 gtaggggagg tcgtcggtct tcggcatgcg cccttgcagc gcaaggttca gcctcgcctg
      481 ccagaaggcg aagttgggga aggagacgat gccgatcttt cctacccgca acatctcctg
      541 cagcaccagc ctaggtttgt gcaccgcctg cagggtctgg ctcaggatga cgtagtcgaa
      601 ggaactgtca gggtaatcct ctataccctc gtccaggtcc ccatgatgga ccgataaccc
      661 gcgggcgatg gagcgataca cgccgtcttc ggagatctcc actcctcggg ccgtcacgcc
      721 ccgctcgttg accagcattg ccatgagatc gccgtcccca cagcccaggt ccagtacgga
      781 ggagcccggc tctaccaggt tgcagatcag cttcaggtcg aacctatcgc tcagaaccgg
      841 aatctccaac ttgtcttctg ctctggtaac catggtcagc gcacctttcc ggacatcgtg
      901 taggagagga agccccgaat caggtgggtc tgggccttct cctcgaggag aaatgcatcg
      961 tggccgtagg ccgacgggat ctcgcagaag ctgacgtcga tgccgttcag tttcagcgcc
     1021 ttcacaattt ccttggcgtg gtaggttggg tagagccagt cggagctaaa ggagatgacc
     1081 aggaagcggg cctggacgtc tctgaaggct gccccgagcg caccggtgcc gttggacagg
     1141 tcgaagtagt ccaccgcctt ggtgatataa aggtagctgt tggcgtcgaa acggtggatg
     1201 aaggcgtctc cctggtggcg caggtagctt tccacctcga agtcggtgga gaagtcgaac
     1261 ccatacgtct ccttattgcg caaccggcga ccgaacttct gtcgcatgga ctcgtcgctg
     1321 aggtaggtga tgtgacccag catgcgcgcg acggcgagac cgggtatcgg gctattcttc
     1381 ccatagtagt cgccgttgtg ccagtgctgg tcggccatga tcgcctgccg ccccacctcg
     1441 ttgaaggcga tgccctgcgc cgatagccgt gctgccgtcg ctatcggtat gcaggcctta
     1501 accctgtccg ggtaagccgc tgcccactgg agtgcctgca tcccccccag cgatccgcct
     1561 atcactgcaa gcagccgccc gatgccgagg tggtcgacga gcgctttctg tgctcgcacc
     1621 atgtcaccca ctgtcacgac cgggaagctg agtccgtagg gtttatcggt tgaggggttg
     1681 atggaactcg ggccggtgga gccacggcag ccaccgagca cgttggaaca gatcacgaag
     1741 tagtgattcg tgtcgatcgc cttgccaggc cctatcatgt tgtcccacca gccgagtcgc
     1801 ttccggtcca ctg
//
LOCUS       DATALS010000442         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_70222,
            whole genome shotgun sequence.
ACCESSION   DATALS010000442 DATALS010000000
VERSION     DATALS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70222"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VHS06_09735"
     CDS             <1..648
                     /locus_tag="VHS06_09735"
                     /inference="COORDINATES: protein motif:HMM:NF018753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HEX2988438.1"
                     /translation="LRARNEMVADPILEAKKCMGDRLLGVVLNSVPLGMIGFATDTLT
                     PFLEKKGVKVYAILPEDKMMMAVTVGELVQRLGGRVISGQDRMDNLVEGVMVGAMGVE
                     NALTYFRRKLNKAVITGGDRPDIQLAALETSTKCLILTGNLYPSPMILGRADELGVPV
                     VLVQQDTLTAAGLVEQSFAEVQFHQPKKIEHFEKVLETHFNYEEFRRDLGLPARA"
     gene            814..>1799
                     /gene="eno"
                     /locus_tag="VHS06_09740"
     CDS             814..>1799
                     /gene="eno"
                     /locus_tag="VHS06_09740"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX2988439.1"
                     /translation="MSTITDVLARQILDSRGNPTVEVEVVLDDGTIGRAEVPSGASTG
                     VHEALELRDQDPAQFGGAGVLKAVENVTDIIAEELAGMSCLDQTGIDQLMIELDGTPN
                     KAKLGANAMLGVSLACAHAAAIFLDLPLYRYLGGANAKTLPVPMLNILNGGKHAENST
                     DLQEFMVMPVGAHSFANAVRMSSEIYHSLKKTLKKKGLNTNVGDEGGFAPSLKSNVEA
                     VEVILEAVTAAGYKPGEEVFIALDPAASELYENGKYNLAREGRTLTSEQMVDFYADWV
                     RQYPIISIEDGLAEDDWEGWAMLTKTIGDKVQLVGDDLFVTNTNRLKRGIQEG"
BASE COUNT          403 a          522 c          552 g          322 t
ORIGIN      
        1 ttgcgagccc gaaatgagat ggtggccgac cccattcttg aagccaagaa gtgcatgggg
       61 gaccgtcttc tcggcgtggt gctgaactcg gtacctttgg ggatgatcgg gtttgccacg
      121 gataccctga cccccttcct cgaaaagaag ggggttaagg tctatgcgat cctgccggaa
      181 gacaagatga tgatggccgt cacagtaggc gaactggtcc aacgcctggg cggccgggtt
      241 atctccgggc aggacaggat ggacaacctt gtcgagggcg taatggtcgg ggctatgggc
      301 gtggaaaacg ccctgaccta cttccgccga aagctcaaca aggcggtgat caccggcggc
      361 gatcggccgg atatccagct ggcggcgctg gagacatcga ccaaatgttt gatactcacc
      421 ggcaatctct accccagccc aatgatcctg ggaagggcgg atgaactggg agttccggta
      481 gtactggtcc agcaggacac cctgacggct gcaggcctgg tggagcaatc atttgccgag
      541 gtgcagttcc accagccgaa gaagatcgag cacttcgaaa aggtgctcga aacccatttc
      601 aactacgagg agtttcgtcg cgacctgggt ctgccagcca gggcgtaagg gcgtaatcca
      661 cgtgtaagcg ggaggaaaac tccctgccgg cacatagctg tttcgcccgt aagcaaccgg
      721 tgggcatcgc cctgccagcc ccacctgacc cgacgtttcg ggcaggatgg ggctgtgtgc
      781 gtcggtcgcg ctagtctgtt ggaggcagta gacatgtcaa ccattaccga tgttctggca
      841 aggcagattc tcgattccag aggcaacccc accgtcgagg tcgaggttgt gcttgacgac
      901 ggcacgatag ggagagccga agtcccatcg ggggcatcca caggggttca cgaggccctg
      961 gagctacgcg accaggatcc tgcccagttc ggcggggcag gcgttctcaa ggcagtcgag
     1021 aacgtcacag acatcatcgc agaagaactg gccggcatgt cctgtctgga ccagaccggc
     1081 atagaccagc ttatgatcga gctggatggg accccgaaca aggcaaagct gggcgccaac
     1141 gcgatgctcg gcgtctccct cgcttgcgcc catgcggcgg caatcttcct ggatcttccc
     1201 ctctaccgct acctgggtgg agccaacgcc aaaaccctcc cggttcccat gctcaacatt
     1261 ctgaacggtg gtaagcacgc cgagaactcc accgatctac aagagttcat ggtaatgccg
     1321 gtgggcgccc acagtttcgc caacgccgta cgaatgagct ccgagatcta ccactccctc
     1381 aagaagacgc tgaagaagaa ggggctcaac acgaacgtcg gcgacgaggg cggcttcgcc
     1441 cccagcctca agtccaatgt cgaagcagtc gaggtaatcc tggaggccgt caccgccgcc
     1501 ggatacaagc ccggcgagga ggtcttcatt gcgctggatc ccgcggcctc cgagctgtac
     1561 gagaatggca agtacaacct ggctcgcgag ggtcgcacgt tgacctccga gcagatggtg
     1621 gacttctacg ccgactgggt acggcaatac ccgatcatct ccatcgagga tggcctggct
     1681 gaggacgact gggaaggctg ggctatgctc acaaagacca tcggcgacaa ggtacaactg
     1741 gttggagacg atctcttcgt caccaacacc aacagactga agagaggcat tcaggaagg
//
LOCUS       DATALS010000443         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_437995, whole genome shotgun sequence.
ACCESSION   DATALS010000443 DATALS010000000
VERSION     DATALS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437995"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VHS06_09745"
     CDS             <1..341
                     /locus_tag="VHS06_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose synthase"
                     /protein_id="HEX2988440.1"
                     /translation="ERQRRDPNSLLNRVERMIRLRKEHPEFGLGEWAVVDTGIPSIFA
                     VRASWKNSTMVAVHNLSRRKCTVSLPLPDDESRHLVDALKGDSIDAPEEGPCQVELEG
                     YGVRWLRVAK"
     gene            complement(403..1389)
                     /locus_tag="VHS06_09750"
     CDS             complement(403..1389)
                     /locus_tag="VHS06_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX2988441.1"
                     /translation="MSSNQKVGYKLVEGWEKLPSGFKHLDVVGAAVDAADRVYIITRS
                     DARVIVYERDGSYVTSWGEGIFTPRTHCIRFGADGSVFTVDDGDHTVRKFTPDGQLLL
                     MLGTPGVASDTGYSKAAGISSIVRGGPPFNRPTGIAIAPNGELYVSDGYGNARVHRFS
                     PEGDLIQSWGEPGTAPGQFNLPHSLWVMPDGRVMVADRENDRIQFFTADGAFLYEWTD
                     VQRPTGVYVDKGGLIYVSELWWKVGQKSFRNGPITEDRPGRVSVFSPEGEVLARWGSS
                     DRTAPGQFIAPHGLCVDSHGDLYVAEVTYTFAAKPGLVPIDSHCFQKFARVS"
     gene            complement(1488..1871)
                     /locus_tag="VHS06_09755"
     CDS             complement(1488..1871)
                     /locus_tag="VHS06_09755"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX2988442.1"
                     /translation="MDGAEVVAGTVFSFYPLVKAGKLRLLAVMDKQESSMAPGVKTAE
                     AQGFKVYGAAARGIYVPAGTPKEVVDVLANTVKKITSDPAYQSKMLEMGHIVRYMGPE
                     ETDKYWSDLEASMKPLIEQAIAESK"
     gene            1870..>2183
                     /locus_tag="VHS06_09760"
     CDS             1870..>2183
                     /locus_tag="VHS06_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988443.1"
                     /translation="MIGRWAPRLALLVILIAYATFLWTRFAPAIATPDANGYWAQASL
                     MAQTGKTWFVQEADTQYVGMHWLITPSGKYISRYPPGLPAIVAVVYKLAGYKASLLVN
                     PVL"
BASE COUNT          407 a          706 c          631 g          439 t
ORIGIN      
        1 tagaacgtca gcgccgggac ccgaactctc tgctgaacag ggtggagcgg atgatccggc
       61 tgcgaaagga gcacccggaa ttcggcctgg gcgagtgggc cgtggtggat accggcatcc
      121 catccatctt cgcggtgcgc gcctcgtgga agaacagcac catggtagca gtacacaacc
      181 tctcccgccg gaagtgcaca gtgtcgctcc ctctgcccga cgacgagtcg cgccacctgg
      241 tggatgcact caagggcgat tccatcgatg ccccagagga gggaccgtgc caggttgaac
      301 tggagggcta tggtgtccgc tggcttcgcg tggcaaagta gggattgccg gtatccacct
      361 gctcgagcgc tacattgaga atagcagtcg cggagccacc gtctagctca ctcgcgcgaa
      421 cttctggaag cagtggctgt ctatgggaac cagtccgggc tttgctgcaa aggtataggt
      481 gacctccgcc acgtagagat cgccgtgaga gtctacgcac agaccatggg gagcgatgaa
      541 ctgccccggt gcggtcctgt cggaactccc ccagcgagcg agtacctctc cttctggcga
      601 gaacacagac accctccccg gcctgtcttc ggtgatcggt ccgtttcgga aggacttctg
      661 gccaaccttc caccacaact ccgagacgta tatcagcccg cccttgtcca catagacgcc
      721 cgttggcctc tgcacgtccg tccactcgta caggaaagcg ccatcggcag tgaagaactg
      781 tatgcggtcg ttctcccgat cggcaaccat gacgcgacca tccggcatca cccagaggga
      841 gtgcggcagg ttgaattggc cgggcgccgt ccctggctcc ccccacgact ggatcagatc
      901 tccctccgga gagaagcgat ggaccctggc gttgccatat ccgtctgaga cgtatagctc
      961 gccgttcggt gctattgcta tcccagtggg cctgttgaac gggggccccc cacgcacgat
     1021 gctcgagatg cccgcggcct tgctgtagcc ggtgtcggaa gcaaccccgg gtgttccaag
     1081 catcaggaga agctggccgt ccggcgtgaa cttccgcacc gtgtgatccc cgtcgtcgac
     1141 ggtgaacacc gaaccgtccg cgccaaaacg gatacagtgg gtgcgaggtg tgaagatgcc
     1201 ctcgccccag gaggtaacgt agctgccgtc ccgctcgtag actatgactc gcgcatcgct
     1261 cctggtgatt atgtagacac ggtccgcggc atccaccgcc gctccaacca cgtcgagatg
     1321 cttgaagccc gacggcagct tctcccaacc ttcgacgagc ttgtacccga ccttctgatt
     1381 gcttgacatt ccgtttaccc cttaaccacg gttgtcccgt tcattgaagg gagggggccg
     1441 cacgaccccc tcccttcaat gaacttgtct cctgtcgtac gcttctacta cttgctctct
     1501 gcgatagcct gctcgatcag aggtttcatc gaggcctcca gatcagacca gtacttgtct
     1561 gtctcttccg gacccatata tcgaacgatg tgccccatct cgagcatctt gctctggtag
     1621 gcaggatcgg aggtgatctt cttcaccgtg ttcgcaagga cgtctacgac ctctttcgga
     1681 gtgccggccg gaacgtagat gccacgagcc gcggcaccgt agaccttgaa cccctgggcc
     1741 tccgccgtct tgacgcccgg tgccatggag ctttcctgct tgtccattac ggccagcagc
     1801 ctcagcttcc cagccttcac cagagggtag aaggagaaga ctgtaccggc cacgacctcg
     1861 gcgccgtcca tgatcggtcg ctgggcgcct cgcctggcac tgctggtgat cctgatcgcg
     1921 tacgccacct tcctctggac ccgcttcgcc ccggcgatcg ccactcctga tgcgaacggt
     1981 tattgggcac aagccagcct gatggcccag acgggcaaga cttggttcgt tcaggaggca
     2041 gacacgcagt acgttgggat gcactggctg atcacgccct cgggcaagta catcagccgg
     2101 tatccgccgg gactgccggc gatcgtcgcg gtggtatata agctggctgg ctacaaggcc
     2161 agcttgctgg tcaaccctgt act
//
LOCUS       DATALS010000444         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_438011, whole genome shotgun sequence.
ACCESSION   DATALS010000444 DATALS010000000
VERSION     DATALS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438011"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VHS06_09765"
     CDS             <1..619
                     /locus_tag="VHS06_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEX2988444.1"
                     /translation="TAATRGRRDTQPERWRRRSTLLSCGIPSCCRFLATDSMLFVNLI
                     VTYFTLRFRSPAWPPDGTPKPELLMAGIATILLRASSGPVQGADYSMAKGHVGRAKLG
                     LAAGFILGSTFLALQVVGALQWTFTLGSSAYSSVFIILTSFHAAHVLTGLIMLALALL
                     LVWMGYYREGHRVGLQNTVLFWHFIDIIWVFVFATLHLSPYGPLH"
     gene            637..1374
                     /locus_tag="VHS06_09770"
     CDS             637..1374
                     /locus_tag="VHS06_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988445.1"
                     /translation="MLQAEGAPGRPSALAAWLGVATGPIAWTIHLVVCYLLVPYACET
                     GNSWVFHMTTGLTGGATFLAGYLAYRAWRQSEVGLEADASDWSGTERLTRRLGFMGIL
                     GLFLSIVFFALILAESAGTVALSPCQNRQYGGEASHQWPDSGRGRLFAQADYSEGGVE
                     PPIASRRAGSCAPPRTPRPRPPQWIPAAVNAGRKTAIPAPCPPPPRRLLPTLWAGHKT
                     PPYGSGVLSPFSLDEPHSTQEESRHAA"
     gene            1364..1657
                     /locus_tag="VHS06_09775"
     CDS             1364..1657
                     /locus_tag="VHS06_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988446.1"
                     /translation="MQPDYLTSTDWLWVASFVEAILWFTLLGIVSGLAFLTAHSVLPS
                     LIANRELAPETRLLQMGAYVASFISLGVALYILAIALGYAFAVIERVFPRWWI"
     gene            1657..2172
                     /locus_tag="VHS06_09780"
     CDS             1657..2172
                     /locus_tag="VHS06_09780"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX2988447.1"
                     /translation="MGGPKLWIAGVVLGIGVLAIAAYGIAFGLNRQHQPPEQPIRFPH
                     QWHIQSAGVECLFCHRTAATGVSGGLPAVEQCMFCHRVAGRQSPEIQKLTTVSDNMQP
                     IDWVHVYRVPDHVRFTHQPHYQAGVDCSSCHGDVKNSTLLVQVQRMKMGFCVTCHRQQ
                     NARTDCWSCHY"
     gene            2184..>2585
                     /locus_tag="VHS06_09785"
     CDS             2184..>2585
                     /locus_tag="VHS06_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX2988448.1"
                     /translation="MTCSRRDFLKIVATSAGGAALFAGCGQLPPGIPPQKEFQLQSAA
                     AIPEDYVTGVDNLYATICQQCTAGCGILVRVVEGRAKKIEGNPNHPVNGPKHCARAEA
                     ALQGLYHPDRLQGPMRRTGERGSGQFQPISWE"
BASE COUNT          455 a          834 c          819 g          477 t
ORIGIN      
        1 gactgccgcc acgcgcggta ggcgagatac ccagccagaa aggtggcgcc gccggtcaac
       61 cctgttgtca tgtggaatac ccagctgttg ccggtttctc gccacggact cgatgctgtt
      121 cgtcaatctg atcgtgacct actttacgct gaggttcagg tcacccgcct ggccgccgga
      181 tggaactcca aagccggaat tgctgatggc ggggatcgcc accatcctgc tgcgggccag
      241 cagcggcccc gtccaggggg cagattacag catggcgaaa ggtcacgtcg gtcgggcgaa
      301 actcgggctt gccgcgggct tcatcctggg ctccaccttc ctcgcgctcc aggtcgtcgg
      361 tgcgctccag tggaccttca ccctgggcag cagtgcgtac agctcggtct tcatcatcct
      421 caccagcttc cacgccgccc acgttctaac cggactgatc atgctggcac ttgcgctcct
      481 tctggtctgg atgggctact accgggaggg acaccgcgtc gggctccaga acacggtcct
      541 gttctggcac ttcatcgaca tcatatgggt cttcgttttc gcaaccctgc acctgtcacc
      601 ctatggaccg ctccactgag gaaccggagg aggaggatgc tgcaagccga gggagcgccc
      661 ggcaggccaa gtgcactggc tgcatggtta ggtgtcgcta ccgggccgat agcctggacc
      721 atccacctgg tggtttgtta cctgttggtc ccctacgcct gtgaaaccgg caacagctgg
      781 gtattccaca tgacaacagg gttgaccggc ggcgccacct ttctggctgg gtatctcgcc
      841 taccgcgcgt ggcggcagtc ggaggtcggg ctggaggccg acgccagcga ttggtcgggg
      901 acggagaggc tcactcgcag gctgggcttc atggggatac tggggctgtt tctgagcata
      961 gtgttcttcg cgctgatcct ggccgaatcg gccggaaccg tggcgctgag tccctgccag
     1021 aaccggcagt atggcggcga ggcgtcccat cagtggcccg acagcgggcg ggggcggctc
     1081 ttcgcccagg ccgactacag tgagggcggc gtcgaaccac ctattgcgtc tcgtagggcg
     1141 gggtcttgtg ccccgcctcg cacaccgcgg ccgcgaccgc ctcaatggat tccggccgcg
     1201 gtgaatgcgg ggcgcaagac tgcgattccc gcaccgtgcc caccacctcc acgccgtctt
     1261 ctgccaaccc tgtgggcggg gcacaagacc ccgccctacg gttcaggagt gctgtcgcca
     1321 ttctccctgg atgaaccaca ctcaacccag gaggagtccc gccatgcagc ctgactacct
     1381 cacgtcgaca gactggctct gggtggcctc cttcgtcgag gctattctgt ggttcaccct
     1441 gttggggata gtatcgggcc tggcgtttct gactgcccac tccgtgctcc cgtcgctcat
     1501 cgccaaccgg gaactggctc cggagactcg cctcctccag atgggggcct acgtagccag
     1561 cttcatctct ctgggggtcg cactgtacat cctggccata gcactggggt acgccttcgc
     1621 cgtgatagag agggtgtttc ccaggtggtg gatctgatgg gtggaccgaa gctgtggatc
     1681 gcaggggtag tgctgggcat cggcgtgttg gccatcgccg cctacggcat agccttcggc
     1741 ctcaacagac agcaccagcc gcccgagcaa ccgatccggt tcccgcacca gtggcacatc
     1801 cagtcggccg gagtggagtg cctcttctgc caccgcacgg ccgcgacggg agtctcaggg
     1861 ggactgcccg ccgtcgagca atgcatgttc tgccatcggg tagcgggtcg acagagcccg
     1921 gaaatccaga agctgaccac ggtgagcgac aacatgcagc ccatcgactg ggtgcacgtt
     1981 tacagggtgc cggatcacgt gcggttcacc caccagccgc actatcaggc cggcgtcgac
     2041 tgctcgagtt gccatggaga tgtgaagaac agcacgctgc tggtccaggt gcagcgaatg
     2101 aagatgggct tctgcgtgac gtgccatcga cagcagaacg cccgcacaga ctgctggagc
     2161 tgtcactact gacgaggatc tgaatgacct gctctcgacg cgacttcttg aagatcgtgg
     2221 ccacctctgc cggcggagcc gcgctcttcg caggatgcgg gcagctaccc cctgggattc
     2281 cgccccagaa ggagttccag ctgcaaagcg ctgccgccat acctgaggac tacgtcacag
     2341 gggtcgacaa cctatacgca accatctgcc aacagtgcac cgccggctgt gggatcctgg
     2401 tccgcgtggt agaggggagg gcgaagaaga tcgaggggaa ccccaaccac ccggtcaacg
     2461 gccccaagca ctgtgcccgc gccgaggccg ccctccaggg actgtaccac cccgacaggc
     2521 ttcaagggcc gatgcgaagg acgggggagc ggggcagcgg acagtttcag ccaatctcgt
     2581 gggaa
//
LOCUS       DATALS010000445         3852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_347126, whole genome shotgun sequence.
ACCESSION   DATALS010000445 DATALS010000000
VERSION     DATALS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347126"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            564..824
                     /locus_tag="VHS06_09790"
     CDS             564..824
                     /locus_tag="VHS06_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988449.1"
                     /translation="MSRRRLGVREQAFLQTGPQGDMVVVILEGTDPASAFRQFGAGND
                     DFTRWFVQQVKEIHGMDLSQPSQLPMPQLGVDSDMQVRRKAA"
     gene            complement(886..1710)
                     /locus_tag="VHS06_09795"
     CDS             complement(886..1710)
                     /locus_tag="VHS06_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2988450.1"
                     /translation="MDSLSGKVTWVTGSSRGIGKAIAAKMASLGSAVVVHGTTPTSAR
                     AFNEGESLDAVARSIASEHGVEVLAVHGDLTDNAQVRRIAGEIRARFGRIDIMVNCAG
                     GDIGAKGTSGPEGGRPEHNDAIFVSDDDIRTVLDRNLMSCILCCREVAPEMMERKSGK
                     IVNLSSIAGLGGTTVGVIYAAAKAAVAEYTRCLAMQLRPYNVMVNAVAPGPILTPRFA
                     ASRALDEEMMAETGTLVRYGRAPEIAAAVAFLASDEASYITGQVLRVDGGHQCWPA"
     gene            complement(1968..2261)
                     /locus_tag="VHS06_09800"
     CDS             complement(1968..2261)
                     /locus_tag="VHS06_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006455857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX2988451.1"
                     /translation="MSTTGCTHLNEIRDVTPGSEGCEECLKTGDSWVHLRICMTCGHV
                     GCCDSSRNKHATKHYHETGHPIIQSFQPGEEWLWCYPDQLFMETAPGKLKHRR"
     gene            complement(2462..>3852)
                     /locus_tag="VHS06_09805"
     CDS             complement(2462..>3852)
                     /locus_tag="VHS06_09805"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HEX2988452.1"
                     /translation="QLDDNLLAAHIFITIPTGGGKVATVDGVKADLAEAGVVFGIDPV
                     ALENAVKEAQALSIPGMRWGPVLVAQGRSPVAGDDGKIRYHPCLTAASGRPKVAPDGT
                     VNLFDLNVVHNVAKGTVLAVETPPTAGQPGMTVMGGEIPARPGRECWLKPGKGTELSE
                     DKLTVTAACDGHATLMHGEISVTNIFQVNRDVGVETGNIQFVGSVVIRGNVQHGFSVK
                     AEGDVEVQGGVHGGTIEAAGNITVQYGIKGPIGHGKVTAGGAVKAKFIEGADVVAGTN
                     VWAADGILQSRVAAGASVEVLGRRGAVIGGHVSAQTSVSARILGSALGSTSEISVGVT
                     PAARTELGENRKKQAELEEQLQRTNQAIQFLAEQERRGLLGPEKRATLAKLGPAQDHI
                     CATLETLKQRVRELEQMFKEGRQAFVEAKETCHPGVTVIIGGSRLSTTAPQSRARFKL
                     NDDQEVEIVAAS"
BASE COUNT          712 a         1279 c         1096 g          765 t
ORIGIN      
        1 caaggatgac gaccaccatc tccccctggg gccccgtctg taagaacgcc tgctcgcgca
       61 cacccaagcg ccgccgggac atctggtagt ccgtgtagcg cggaccgttc aactcctcgg
      121 cgaatctttt ccactgctcg gtcttgcccg gcaggatggg cacagcccaa cccatcaacg
      181 acattccact ttcctttctc gacttagaca gcttgctggg tacaggatgc cggagcgagc
      241 ccggcgaaga gccggcagca ggtttcggac caccgccagg acgtcgatat taaccctata
      301 taccatataa atatagcgct atactatagg gctcgggcga agcgacgtgg cagcccgagg
      361 cgtggctcca ctcctgcact tcttgcagcc ggtggacgac cctaccaatg aatcgggagc
      421 tgttgtcatg accaagcctc gaaccatccg agagttgcgc gccggcgacc atcgtgtcct
      481 ctccgtcaag gaggagcttc gcaagaacct tatccagaag ttgcgtcggg gagttgaatg
      541 gcccgcgcca cgcggaatac cagatgtcca ggcggcgttt gggtgttcgc gagcaggcct
      601 tccttcagac cgggccccag ggggatatgg tggtcgtcat ccttgagggc accgaccccg
      661 cgagtgcctt ccgccaattt ggcgcgggca acgacgactt cactcgatgg ttcgttcagc
      721 aggtgaagga gatccacgga atggatctct ctcagccgtc tcagttgccc atgccccagt
      781 tgggagtcga ctccgatatg caggtgcgga ggaaagcagc gtagggcaac agacagatag
      841 ggcaggccgt ctccggcctg ccctgcaacc cgattggcag cgactctacg cgggccagca
      901 ctggtggccg ccgtccactc gcaacacctg tccggtgatg taggatgcct cgtcggaggc
      961 gaggaatgcg acggccgcgg cgatctccgg cgcacgccca tagcgaacca acgtcccggt
     1021 ttccgccatc atctcctcat ccagcgcccg gctcgcggcg aagcgcggag tgaggatcgg
     1081 gccaggtgct accgcgttga ccatcacgtt gtagggacgc agctgcatcg caaggcagcg
     1141 tgtgtactcg gcgacggcgg ccttggcagc cgcgtagatc acccccaccg tcgtcccgcc
     1201 caggccagcg atgctgctga gattcactat cttgccggat ttgcgttcca tcatctccgg
     1261 agctacttcc cgacaacaga ggatgcagga catcagattt cgatcgagga ccgtgcggat
     1321 atcgtcgtca gagacgaaga tcgcatcgtt atgctcaggg cgaccgccct ccggccccga
     1381 ggtccctttg gcgccgatat ccccgccggc acagttgacc atgatgtcga tgcgcccgaa
     1441 cctggcgcgg atctcccctg cgatccgcct cacctgcgcg ttgtcggtga gatcgccgtg
     1501 gactgccagc acctccaccc catgttccga tgcgatggac cgagcaactg cgtccaacga
     1561 ctccccttca ttgaatgctc gagcggaggt cggggtggta ccgtgaacta cgacggcact
     1621 ccccaagctc gccatctttg cggctatcgc ctttccgatc ccccgcgagg accccgtgac
     1681 ccaggtcacc ttcccactga gactgtccat cactcttccc ctcctcacca ggatgcctga
     1741 tgactgcccg ctgattatag cccagtgacc ggcattcgcc atcactgcgg gagcaaatgc
     1801 cggcgcattg aatggattgt gactgtatgc gcaaagggaa ttgctgtcag gtaccctggt
     1861 ggaatcggag agaatggcgc gaagaggagt ctagacgcgg cacgtgacag gggcgacgca
     1921 cgcgccgccc ctgctcaaca ggcgcgactc ggatggccaa cccctggcta tctcctgtgc
     1981 tttagctttc cgggcgccgt ctccatgaag agctggtccg gatagcacca gagccactct
     2041 tctccgggct ggaaggactg aatgatgggg tggccggtct cgtgatagtg ctttgtggcg
     2101 tgcttgttcc tggaggaatc acagcagccg acgtggccac aggtcatgca gattcgcagg
     2161 tggacccagc tatcccccgt cttcagacac tcctcgcaac cctcgctgcc gggcgttacg
     2221 tcgcggatct cgttcaggtg ggtgcagccg gtggtgctca tctcccctcc atctgcaatg
     2281 aagcatccga accgatggct caccgtcacc ggtcctcgcc ccggcgtggc ggggcgctcg
     2341 ctctctcctc cggcaccgac cgtgccaaga ggcagatgct gagggatgtc tcaccctctt
     2401 gagagaggat tcctgagagg ctgatgtcgg gggatgggga aatggggtcc cggtagacag
     2461 gctaagaggc tgccacgatc tcgacctctt ggtcatcatt cagtttaaat cgcgctcgcg
     2521 actgcggggc ggttgtgctc aagcgagagc cgccgataat cacggtgact ccaggatggc
     2581 aagtctcctt cgcctcgacg aatgcctggc gcccctcttt gaacatctgc tccaattccc
     2641 gaacacgctg cttcagcgtc tccagagtgg cacagatatg atcttgggcc ggacccagtt
     2701 ttgccagagt tgcacgcttt tccggcccca agaggccacg ccgctcctgc tccgccagaa
     2761 actgtatagc ctggttggtc cgctgcaatt gctcctccaa ctcggcctgc ttcttccggt
     2821 tctcgcccaa ctccgttctg gccgcagggg taaccccgac cgagatctcc gaggtgcttc
     2881 cgagggcaga gcctagtatt cgcgccgaca cgctcgtctg agcagatacg tgtccaccta
     2941 tgactgcacc acgacgcccc agcacctcca ccgaagcacc ggcagccact cggctctgca
     3001 gtattccgtc tgccgcccac acgttggtgc ctgccacaac atccgcaccc tcgataaact
     3061 tggccttcac ggcgccaccc gccgtcacct tgccgtgacc tatgggacct ttgatcccat
     3121 actgcacggt aatgtttccg gcagcctcga tggtgccacc atgcactccg ccctgaactt
     3181 ccacgtctcc ctcggccttg acgctgaaac cgtgctggac attgcccctg atcacaacgc
     3241 tgcccacgaa ctggatgttg cctgtttcga ctcccacatc cctgttcacc tggaatatgt
     3301 tggttacaga gatctctcca tgcatcaagg tcgcgtggcc atcgcaggcc gctgtgaccg
     3361 ttagcttgtc ttcgctcagc tcagtgccct tgccgggctt gagccaacac tcccggccgg
     3421 gcctcgcggg gatctcgccg cccataaccg tcataccggg ctgtcccgcg gttggagggg
     3481 tttccacggc caagacagtg cccttggcca cgttgtgaac cacgttcagg tcgaagagat
     3541 tgaccgtgcc atccggtgcc accttgggac gaccgctggc agcagtcagg caagggtggt
     3601 acctgatctt accgtcgtct ccagccaccg gggacctgcc ctgggccacg agaacgggac
     3661 cccatctcat gccgggaatg ctaagagcct gggcctcttt gaccgcattc tccagtgcaa
     3721 ctgggtcaat cccgaagacg actccagcct cagccagatc tgccttgacc ccatcgacgg
     3781 tcgccacctt cccgccaccc gtggggatag tgatgaagat gtgagccgcc agcaagttgt
     3841 cgtccaactg ga
//
LOCUS       DATALS010000446         4609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_438373, whole genome shotgun sequence.
ACCESSION   DATALS010000446 DATALS010000000
VERSION     DATALS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438373"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VHS06_09810"
     CDS             <1..740
                     /locus_tag="VHS06_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX2988453.1"
                     /translation="PCRAPDPPFPAVVGLYGKPTVINNVETLSNVPHIINRGVEWYNS
                     IGVAPRNTGTKIYCLSGCVNNPGNYELPLGTTIRELIYEHGGGVLEGRALKAVIPGGT
                     SSPMLGPDMLDLPLEFDAVAKAGSMLGSAAVIALDESVCIPHAVARMVEFYEHESCGK
                     CTPCREGTRWLSKILDRIVEGHGRDGDIDLLLDVCDNIAGGKTLCGLGDAATNPIRSG
                     MRLFREEFEYHIKQGRCMVGGGVVSHG"
     gene            733..2997
                     /gene="nuoG"
                     /locus_tag="VHS06_09815"
     CDS             733..2997
                     /gene="nuoG"
                     /locus_tag="VHS06_09815"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX2988454.1"
                     /translation="MADLVKVTIDGREVLVPKGTLLVEAAKQAGVEIPVFCYHEKMHP
                     VGACRMCLVEIEKMPKVQTACTTPVSDGMVVHTDSPMVEKARKGVMEFLLCNHPLDCP
                     VCDKGGECPLQNTTFKYASDRSRFGEEKRHFVKPIRLSEKIVLDRERCIMCSRCVRYV
                     REIAGDESLTLVSRGSRSYVGVMPGRSFDSPFSGNTIELCPVGALTSAKFRFRARVWE
                     LKETPTVCAQCSVGCNIKVGSRNGELLRLTSRENTPVDDGWLCDRGRFTYQFVNSPER
                     LTTPMIRRDGKLVPASWADAVEAVSRRLRQMRESYGSESLGGMISARSTNEDLYLFQK
                     LFRSLLGSQNLDHGLHGQYVQPEGGVDAASGSIEGLESAQIVLLVDVDPINQQPVLDL
                     RLKKAWQEKRAKLVSIGARETDLTKRSSSWLVTEEGAEAAVVKGILHIMLTEGLLGDA
                     ARERLGDRYDVVAQSVSEFAPERVQEAAGVAPEAIRKVAGLLAGTTKVAVLFPRPLPG
                     APAGLQEACGWLASATGCLTEPGSGGLFPLGLECNSQGAIDMGVLPNLLPGQQKSGSA
                     GLSGLQMVKAAAEGQLKALYMVGLDPVGQEDAEQVRAALSKLELLVVQDIFLTETAKI
                     ADVVLPGASFAEKDGSYTNLERRVQRLNGAIPCPGEALQDWEIIAAVGNGLGAGWDFR
                     KAAEVFDAMVAETPLYSGLSFDVLGDKGQQWSYPGALSAQPEHNGRERHLWYRRLPAE
                     EPESVEVREQEPSV"
     gene            2997..3992
                     /gene="nuoH"
                     /locus_tag="VHS06_09820"
     CDS             2997..3992
                     /gene="nuoH"
                     /locus_tag="VHS06_09820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX2988455.1"
                     /translation="MELILAILIALVKSIVVFFVLVLAAAYSTLLERKELAWMQVRVG
                     PNRVGPKGLLQPIADAVKAIFKEDFAPEGADKVVYWLAPAIAMMASMFSFVVIPFGPS
                     FELFGFPIDLQIADVSVGVLVLLAVSSLGVYGIVLAGWSSNSKYSLLGSVRSSAQMVS
                     YELGMGLSILSVVVTAGSLRISDIVAFQQTMPMVLIQPLAFVMYIVCALAENARAPFD
                     LTEAEQELVAGYNTEYSGLKFAMFYLAEYVSMVTSGAVATTLFLGSYHGPFLDGPWWF
                     IIKVFAIVFCFIWARATLPRLRYDKLMRLGWQFILPVTLLNMVVTAVVVAMTSAA"
     gene            4010..4528
                     /gene="nuoI"
                     /locus_tag="VHS06_09825"
     CDS             4010..4528
                     /gene="nuoI"
                     /locus_tag="VHS06_09825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX2988456.1"
                     /translation="MASGSVVQDVRDILSMAKGFSITFRHMFRPKHTIQYPEQKRATM
                     PRQRWRHNLHRYANGMERCIGCSLCAAACPAGAIFVEPAENTDADRHSPGERYAKRYE
                     INLLRCIFCGYCEEACPTQAIVLGQEYELVDDDRGDFIYTKERLIVAPEKGHGEAPVG
                     VRSLPYVQEVLK"
BASE COUNT          841 a         1269 c         1631 g          868 t
ORIGIN      
        1 ttccatgccg cgctccagac ccccccttcc ctgcagtggt ggggttgtac ggtaagccga
       61 cggtaatcaa caacgttgag accctctcca acgtacccca tatcatcaat cgcggcgtgg
      121 agtggtacaa ctccatcggg gttgcacccc gcaacaccgg taccaagatc tactgcctga
      181 gcggctgcgt gaacaacccg ggcaactacg aacttccgtt ggggacgacg atcagggagt
      241 tgatctacga acacggcggc ggcgtgctgg aggggagggc cttaaaggcc gtcatccccg
      301 gcggtacatc ctcgccgatg cttggtcccg acatgctgga ccttccgctg gagttcgacg
      361 cggttgccaa ggccggatcg atgcttggct ccgctgcggt gatcgcgctg gacgagagtg
      421 tgtgcatccc ccacgccgtg gcccggatgg tcgagttcta cgaacacgag tcgtgcggga
      481 agtgcacccc ctgccgcgag ggcacccggt ggctctccaa gatcctggat cggatcgtcg
      541 aggggcatgg gcgggatggc gacatcgacc tgctgctgga tgtgtgcgac aacatcgcgg
      601 ggggcaagac gctctgcggc ctcggggacg ccgcgaccaa ccccataagg agcgggatga
      661 ggctgttcag ggaggagttc gagtaccaca tcaagcaggg gcgctgcatg gtcggtggcg
      721 gggtggtgag ccatggctga tctggtgaag gtgacgatag acggccgaga ggtgctggtg
      781 cccaagggca cgctcctcgt ggaagctgcc aagcaggcgg gcgtggagat ccccgtcttc
      841 tgctaccacg agaagatgca ccccgtcggg gcgtgccgaa tgtgcctggt tgagatcgag
      901 aagatgccca aggtacagac ggcctgcacg actcccgtct ccgacggcat ggtggtccac
      961 accgacagtc cgatggtcga gaaggcgcgg aagggcgtga tggagttcct gctgtgcaac
     1021 catccgctgg actgccccgt gtgcgacaag ggcggcgagt gccccctgca gaacacgacc
     1081 ttcaagtacg cctcggatcg gtcccgcttt ggtgaggaga agcgtcactt cgtgaagccg
     1141 atccggctga gcgagaagat agtgctggat cgcgagcgct gcatcatgtg cagccgctgc
     1201 gttcgttacg ttcgagagat cgccggcgac gagagcctga cgctggtcag ccgcgggtcg
     1261 cgcagctacg tgggggtgat gccggggcgc agcttcgact cgccattttc cggcaacacg
     1321 atcgagcttt gcccggtggg tgcgctcacg agcgcgaagt tccgcttcag ggcccgtgtg
     1381 tgggaactga aggagactcc gacggtgtgc gcgcagtgca gcgtggggtg caacatcaag
     1441 gttggatcga ggaacggtga gttgttgcgg ctcacgtcgc gggagaacac gcccgtggac
     1501 gacggctggc tatgcgacag ggggcgcttc acctaccagt tcgtgaacag ccctgagcgg
     1561 ctaacgacac cgatgataag gcgcgacggc aagctggttc cggcaagctg ggcggacgcg
     1621 gtggaggctg tgtctcgccg gctgcgacag atgcgggaga gctacggcag tgagagcctg
     1681 ggcggcatga tctccgcccg ctccaccaac gaggatctct acctgttcca gaagctgttc
     1741 cggtcgttgc tggggagcca gaacctggat catgggctcc atggtcagta tgtgcagccc
     1801 gagggcggcg tggatgccgc aagcggcagc atcgagggac tggagtcggc gcagattgtc
     1861 ctgctggtgg acgtggaccc gatcaatcag cagcctgtgc tcgacctcag gttgaagaag
     1921 gcgtggcagg agaagagggc gaagctggtc agcatcgggg cgagggagac cgacctgacc
     1981 aagcggagca gcagctggct tgtcacggag gagggcgccg aggcagcggt agtgaagggt
     2041 attcttcaca taatgctgac cgaggggctg cttggggatg ccgccaggga gcggctgggc
     2101 gaccggtacg atgtggtggc tcagtcggtg tcggaattcg ccccagagcg agtgcaggag
     2161 gccgcgggcg tcgctccaga ggctatccgc aaggtggcgg gactgcttgc cggcacgacg
     2221 aaagtggcgg tgctgttccc cagaccgctg cctggcgcac cggcagggct gcaagaggcc
     2281 tgtggctggc ttgcttcggc cacagggtgc ctcacggagc cgggttcggg cgggttgttc
     2341 cccctgggac tggagtgcaa ctcccagggt gccatcgaca tgggggtgtt gccgaacctt
     2401 ctacccggcc agcagaagag cggcagcgcg ggattgagcg ggctgcagat ggtgaaggca
     2461 gccgctgagg gccagttgaa ggcgctttac atggtgggtc tcgatcccgt tgggcaggaa
     2521 gatgccgagc aggtgagggc ggcgctgagc aagctggagc ttctggtggt ccaggacatc
     2581 ttcctcaccg agactgccaa gatagcggat gtggtgctgc ctggtgcgag cttcgccgag
     2641 aaagatggca gctacaccaa cctggagcgg cgggttcagc ggctgaatgg tgcgatacct
     2701 tgtccgggcg aggctctgca ggattgggag atcatcgccg ccgtcggcaa cggactgggg
     2761 gcgggatggg acttcaggaa ggctgcggag gtcttcgacg ccatggtggc ggaaaccccg
     2821 ctctactcgg ggctgagctt cgatgtcctt ggtgacaagg ggcagcagtg gagctatccg
     2881 ggtgctctga gtgcccagcc cgaacacaac ggtcgggagc ggcacctctg gtatcggcgg
     2941 ctgccggcag aggaaccgga atctgtggaa gtacgggaac aggagccaag cgtctgatgg
     3001 agctaatcct ggcgatcctg atagccctgg ttaagtcaat agtcgtcttc ttcgtgttgg
     3061 tcctcgcggc ggcctactcc acgttgctgg agaggaagga gctggcgtgg atgcaggtca
     3121 gggtgggccc gaaccgtgtt ggcccgaaag ggctgctcca gccgatagcg gacgccgtca
     3181 aggcaatatt caaggaggac ttcgcgcccg aaggcgcgga caaggttgtg tattggctgg
     3241 ctccggccat agccatgatg gcctccatgt tcagctttgt cgtgatcccc ttcggcccga
     3301 gcttcgagtt gtttggcttc cccatcgacc tgcagatcgc cgacgtgagc gtgggggttc
     3361 tggtattgct ggccgtttcc tccctggggg tttacggcat cgtgcttgcc ggttggtcgt
     3421 cgaacagcaa gtactctctg ctggggtcgg ttcgctcctc cgcgcagatg gtcagctacg
     3481 agctgggcat gggactctcg atattgagtg tggtggttac ggccggcagt ctgcggatct
     3541 cagacatcgt ggccttccag cagactatgc ccatggtgtt gatccagccg ctggccttcg
     3601 tgatgtacat agtatgtgca ttggcagaga atgctcgtgc gcccttcgac ctaaccgagg
     3661 cggagcagga gttggtggcg ggctacaaca ccgagtactc agggcttaag ttcgccatgt
     3721 tctacctggc cgagtatgtg agcatggtga cttcgggcgc tgtggcgacg accctgttcc
     3781 tgggcagcta ccacgggccg ttcctggatg ggccgtggtg gttcatcatc aaggtgttcg
     3841 cgatagtgtt ctgcttcatc tgggccaggg cgactctccc caggctcagg tacgacaagc
     3901 tgatgaggtt gggctggcag ttcatcctgc cggtgacgct tctgaacatg gtggtgaccg
     3961 cggtggtggt tgccatgacg tcggcggcat aggggatatt gaggaactga tggcaagcgg
     4021 tagcgtggta caggacgtca gagacatact gagcatggcg aaggggttct caatcacatt
     4081 ccggcacatg ttcaggccca agcacaccat ccagtacccg gagcagaaac gggcgacgat
     4141 gccgcgccag cggtggcggc acaacctgca taggtatgcc aatgggatgg agcgatgcat
     4201 cggctgctcc ctctgtgcgg cggcctgtcc ggctggagcc atattcgtgg agccggcgga
     4261 gaacaccgat gcggataggc actcccccgg tgagcggtac gcgaagcggt acgagatcaa
     4321 cctgctgcgc tgcatcttct gcggctactg cgaggaggct tgcccgactc aggcgatcgt
     4381 gctggggcaa gagtacgagc tggtggacga cgatcgaggg gacttcatat acaccaagga
     4441 gcggctgatc gtggccccgg agaagggaca cggcgaggcc cccgtggggg tgcggagcct
     4501 cccctacgta caggaagtgc tgaagtaatg gaactcgtct acttcttctc tgtcgtaatc
     4561 gtggcggtgc tctcggcgat cgccatgctg attagccgga acaccgttc
//
LOCUS       DATALS010000447         5357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_118364, whole genome shotgun sequence.
ACCESSION   DATALS010000447 DATALS010000000
VERSION     DATALS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118364"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..2195
                     /gene="pepF"
                     /locus_tag="VHS06_09830"
     CDS             156..2195
                     /gene="pepF"
                     /locus_tag="VHS06_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX2988457.1"
                     /translation="MSETKDPDSGCDNTVSRRQLLKGIGCLAAGTALLPVLGNQENAL
                     AQTVPGDSVAATHSPAATAPPAVTTPAPATAAPVTQQVSTLPDRRDIAPEYTWSLEDI
                     YADADTWEADFQRVRESIPQLEQFKDTLRESPRRLLDYLSLRDDTSNTLDRVYVYANM
                     KKDEDNTNGTYQALADRAAVLGADLGKATAFFSPELLAMPDETLEAFLATEPGLEIYR
                     HALDDLRRQRPHVRTTEVEEVLAQASEMARAAGNSYTMLAEADIKFPSILDEQGRTVD
                     LTQSRYSQFRICPDRRVRKEAFQGIHQTFEKFRNSFASTYSSQVKADIFAAKTRRYGS
                     CLESALADDNVSADVYHNLIDTVTANLPTLHRYLALRKRLLGLSDLHFYDLYVPMVPG
                     VKMEIPYQEATSKVVEALHPLGQEYVDALSDGINSRWIDVYENEGKTSGAYSWGSYST
                     HPYILLNYQGTLNDVLTLAHELGHAMHSYFRTKSQPYVYGGTSIFVAEVASILNEVLL
                     ADYLAKTTNDRRLQAYLINQRLEGLRGTLYRQTMFAEFELEAHSRAEAGVSLTADRLS
                     AIYGELSSKSFGDVVAMDNEIAIEWAVIPHFYTPFYVYKYATGISAATALAQSILTEG
                     EPAVEAYLNLLKAGTSKYPLDLLRDAGVDMASPAPIQKALDSFSDLIDQLESLTA"
     gene            complement(2253..4364)
                     /locus_tag="VHS06_09835"
     CDS             complement(2253..4364)
                     /locus_tag="VHS06_09835"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX2988458.1"
                     /translation="MNLVWIVPVAGVVTILFALYLAWDVLRRDTGSSAMLEVASAIFE
                     GAMAFLKRQYRTILLLALVTSVLIGALIGWVEGNSDLGVKTAISFLVGAGCSALAGFI
                     GMYVAVKSNIRTASAAQRSLAEAITVGMRGGAVSGFLVIAMSLLGVAGIFYAFGGAQF
                     PDKAPFLIVGFGFGASFVALFAQLGGGIYTKAADMGADLVGKVEAGIPEDDPRNAAVI
                     ADLVGDNVGDCAGRGADLFESTAAESIGAMILGVAIYTYAKAQGMANAEDWILFPLVV
                     RAFGSLASMVGIMFVGGGDEHNPMKALNRGYYVAAGLSAIFLVFVVNQMLGNMMFAWA
                     GLVGIATSIAFVYITQYYTSGAWRPVREIAEASKTGPATNIVAGLAVGFECTASTAIT
                     ICIALLASYGLGAASGLPHGGIYATAVATMGMLMTCAYILSMDTFGPITDNAGGINEM
                     AHAPAEARRITDSLDAVGNTTKALTKGYAIASAALAAFLLFSAYMDKVAEVMASRGMT
                     GGFTVVNLAKVDVFVGALLGAMLVYLFSSFAIRAVQRSASAIIEEVRRQFRTDPGIME
                     GTSKPDYARCVDLTTRAALKEMVLPGVLAIGLPVVVGLTLKAEATAGLLMVGTIAGIM
                     LATVLNNGGGAWDNAKKYIEAGYMKIGDEVVAKKGPIHAAAVVGDTVGDPFKDTAGPS
                     LHVLVKLLSTVTLVLAPLFIG"
     gene            4799..5062
                     /locus_tag="VHS06_09840"
     CDS             4799..5062
                     /locus_tag="VHS06_09840"
                     /inference="COORDINATES: protein motif:HMM:NF015996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEX2988459.1"
                     /translation="MYSSIGSWVSGLLTVVGALNWGAVGIFGVNPVHELTGKSRMAER
                     LTYSAVGLAGVFLVFTVMRGSRGSKEVSQLREEVERRRPVGVI"
     gene            complement(5168..>5357)
                     /locus_tag="VHS06_09845"
     CDS             complement(5168..>5357)
                     /locus_tag="VHS06_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2988460.1"
                     /translation="SRADAITAINGQIAEKEEELRQIRLRAEAALGGKPAGPVVCECG
                     TTLAEGAKFCPNCGKRAS"
BASE COUNT         1165 a         1712 c         1560 g          920 t
ORIGIN      
        1 cgggcgggtc aggggcgacc cgccctacta tgtgactgca cctgcagggc acgcccaact
       61 catatttggt ccctctccac taccctggca caaaacgccc atgtgcgcca taattgcctc
      121 cactcatcct ctacttggat gcccgggagg tacttatgtc cgagacgaaa gacccagatt
      181 ccggctgcga caacacggtc agccgacgcc aactactcaa gggtatcggc tgccttgcgg
      241 ccgggaccgc gcttctcccc gtcctaggaa accaggagaa cgccctggcg caaacggttc
      301 ccggggattc tgtcgcggcc acccattcgc cggccgcaac tgcgccgccg gcggttacca
      361 cacccgcccc tgccacggct gcacctgtca ctcaacaagt ctccacgctg ccggatcgga
      421 gagatattgc gccggagtat acctggtcgc tggaggacat ctacgcggac gcagacacgt
      481 gggaggcaga cttccagaga gtacgagaaa gcatcccgca actggagcag ttcaaggaca
      541 cacttaggga gtcgcctcgc cgcctcttgg attacctctc cctccgagac gacaccagca
      601 acaccctgga cagggtttac gtctacgcca acatgaagaa ggacgaggac aacaccaacg
      661 gcacctacca ggcgctcgcc gatcgcgctg cagtcctcgg ggccgacctc ggcaaggcaa
      721 ccgccttctt cagccccgaa ctgctggcca tgccggacga gacgctggaa gcctttcttg
      781 ccacggaacc cgggttggag atctatcgtc acgcgctgga cgacctccgc cgacaacggc
      841 cacacgtccg aaccacggag gtggaagagg tcctcgccca agcatccgaa atggcacgcg
      901 cggccggcaa tagctacacg atgctggccg aagccgatat caagttcccc tccattctgg
      961 atgagcaggg gcgtactgtc gacctgaccc agagtcgcta ctctcagttc aggatctgcc
     1021 ctgaccgcag agtacggaag gaggcgttcc aggggatcca tcagaccttc gaaaagttcc
     1081 gtaacagctt tgcctccacc tactcgtcgc aggtgaaggc agacatcttc gccgctaaga
     1141 cccgccgcta cgggagttgt ctggaatcgg cgctcgccga cgacaacgtg tcggctgacg
     1201 tctaccacaa cctgatcgac actgtaacgg caaatctgcc tacgctccac cgctacctgg
     1261 cgctgcgaaa acggctgcta gggctctccg atctccactt ttacgatctc tacgtgccca
     1321 tggtgccagg ggtcaagatg gagatcccat accaggaggc cacgagcaag gtggtggagg
     1381 cactgcaccc cctgggccag gaatacgtcg acgccctgtc cgatggcatc aattcgcgct
     1441 ggatcgacgt ctacgagaac gaggggaaga ctagcggggc ctacagctgg ggatcctact
     1501 ccacccaccc ctacatactg ctcaactacc agggcaccct gaacgatgtc ctcacccttg
     1561 cccacgagtt gggccatgcg atgcacagct acttcagaac gaagagccag ccatacgtct
     1621 acgggggcac ctcaatcttc gtcgcggaag tggcatccat cctcaacgag gtgctgctcg
     1681 ccgactacct ggccaagacg acgaacgacc gcagactaca ggcgtacctg atcaaccagc
     1741 gactggaagg cctccgcggc accctctacc gccagaccat gttcgcggag ttcgaactgg
     1801 aagcccactc gcgtgcagag gccggcgttt cgctaaccgc agaccgtcta tccgcgatct
     1861 acggggaact gagctccaag tccttcggag atgtggtggc gatggacaac gaaatcgcca
     1921 tcgagtgggc ggtgataccg cacttctaca cgcccttcta cgtgtacaag tacgccacgg
     1981 ggatctccgc cgctaccgcc ctcgcccaat cgatcctgac cgagggcgag ccggcggtcg
     2041 aggcatactt gaacctgctg aaggcaggga cttccaagta tcccctcgac ctgctgaggg
     2101 atgcaggtgt ggacatggca tctcccgctc cgatccagaa ggcgctggac agcttcagcg
     2161 acttgattga ccagttggag agtctcacgg cgtaaccatg acagaaaggg ggccggcgga
     2221 aaaccgccgg ccccctttct gattctctcg acctagccga tgaacagcgg cgccaacacc
     2281 aacgtgacgg tgctcagaag cttcaccagc acgtgcaggg aaggtccagc agtgtccttg
     2341 aagggatcgc caaccgtatc gcccacgacg gccgccgcgt ggatcgggcc cttcttggcc
     2401 acaacctcgt cgccgatctt catgtagccg gcttcgatgt acttcttagc gttatcccag
     2461 gctccaccac cgttgttcag tacggttgcc agcatgatgc cggcgatggt gccgaccatc
     2521 agcagaccgg cagtcgcctc cgccttcaaa gtaaggccga cgacaaccgg aaggccaata
     2581 gcgaggacac caggcagcac catctccttc agagcagccc gggtggtgag gtccacgcag
     2641 cgagcgtagt cgggcttgga ggtaccctcc ataatgcctg gatcggtgcg gaactgacgc
     2701 ctcacctctt cgattatggc actggcggac cgctgcaccg ccctgatcgc gaaggaacta
     2761 aacaggtaga ctagcatagc acccagcagc gcgccgacga atacgtcgac cttggccagg
     2821 ttcaccacgg tgaagccgcc ggtcatgcca cgggaggcca tgacctcagc taccttgtcc
     2881 atgtaggcgc tgaagagcag gaatgccgcc aacgccgcgg aggcgattgc gtagcccttg
     2941 gtgagcgcct tggtggtgtt gccgacggca tccagggaat cggtgatccg ccgagcctca
     3001 gccggggcgt gagccatctc gttgattcca cctgcattgt cggtaatcgg gccgaaggta
     3061 tccatggaga ggatgtaggc gcaggtcatc agcatgccca tcgtggcgac ggcggtagcg
     3121 tagatcccgc cgtggggaag gccactggcc gcaccgaggc cgtaagaggc caggagagcg
     3181 atgcagatgg tgatggcagt gctggcggtg cactcgaagc caactgccag accggcaacg
     3241 atgttggtcg cagggccagt cttggatgcc tcggcgatct cacgcactgg ccgccacgcg
     3301 ccggaagtgt agtactgggt gatgtagacg aaggcgatgc tggtggcaat accaaccaag
     3361 ccggcccagg cgaacatcat gttacccagc atctggttca cgacaaagac caggaagatg
     3421 gctgagagtc cggccgccac gtagtagccg cggttgagcg ccttcatagg gttgtgctcg
     3481 tccccgccac ccacaaacat tatgccaacc atagaggcaa gggagccgaa ggctcggacg
     3541 accaggggaa agaggatcca gtcctcggcg ttggccatgc cctgtgcctt agcataggta
     3601 taaatggcga cgcccaggat catggcgccg atgctctcgg cagccgtcga ctcgaacagg
     3661 tccgcgccac ggccggcgca gtcgccgacg ttgtcgccca ccaggtcggc gataacggcg
     3721 gcgtttcgag gatcatcctc ggggatacca gcctcaacct tgccaaccag gtcggcaccc
     3781 atgtcggctg ccttggtgta gatcccgcct cccaactggg cgaaaagcgc cacgaagctg
     3841 gcgccgaagc cgaagccaac gatgaggaac ggagccttgt cggggaactg cgccccgccg
     3901 aaggcgtaaa agatgcccgc aacgcccagc aggctcatgg cgatgaccag gaaaccggag
     3961 acggcaccgc cccgcatccc cacggtgata gcctcagcga ggctccgctg agcggctgaa
     4021 gccgtccgaa tattggactt cacggcgacg tacatgccga tgaagcctgc caaggcagag
     4081 caacccgccc caacgaggaa cgagattgcg gtcttgacgc ccaggtcgga gttgccttca
     4141 acccaaccga tgagcgcccc gatcagaacc gacgtcacca gcgcgagcag cagaatggta
     4201 cggtactgcc gcttcaggaa agccatcgct ccctcgaaga tggcgctagc cacctccagc
     4261 atggccgagc tgccggtgtc gcggcggagc acatcccatg caagatagag agcgaacagg
     4321 atggtcacca cgccagcaac agggactatc cagaccaagt tcatgacgct cccagacccc
     4381 ctatttggat attattgcgt tcggccacca gccgaacaga cggcgcacga ctcagaccaa
     4441 tggggctcga caagcccgac tatactcgaa acatgcggcc caagtgccat ctgcctgtca
     4501 accaatttgc ctaaccgcca cagtccatct atgcctaaac agtgagaaaa actctcagag
     4561 cgggcagact tatatcggct tattcaggtg atcgtcatgt tagcacagtc tctgaatata
     4621 ccgctactac atgcgttaca aaagtcacaa cacccactat ctgccttgct gaaaagatca
     4681 agatacggtc caggccgttt gtcttggaac gaatgctgct gagccggggc cacctgctcg
     4741 agggaaagcg aagcgactcg aaattgggaa caggcagaga caacggagga ctgcagaagt
     4801 gtactcttcg atcggtagtt gggtgagtgg actcctcacc gtcgtgggcg cgctgaactg
     4861 gggcgccgtg ggcatattcg gagtcaatcc agtccacgaa ctcactggaa aatcgaggat
     4921 ggccgagcgg ctgacctact ccgctgtagg actcgcagga gtatttctcg tattcaccgt
     4981 catgaggggc tcccgcggca gcaaagaggt ctcccagctc cgtgaggaag tggagagaag
     5041 gcgcccggta ggtgtcatct agcgtcctgg tgcacggccg agcggtttgg tggtcgaaaa
     5101 gatcaggcgg gacgcgcccc ggggcgcgtc ccgcctcagc gtcacctatg ggtaagaaac
     5161 gctccggcta agaggccctc ttgccgcaat tggggcagaa cttggcacct tccgccagtg
     5221 tcgtaccaca ttcgcagacc accggacctg ccggcttgcc tcccagggcg gcctctgccc
     5281 tgagccggat ctgccgcagc tcctcttcct tctccgcgat ctggccgttg atcgctgtga
     5341 tggcgtctgc gcggctc
//
LOCUS       DATALS010000448         3530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_118470, whole genome shotgun sequence.
ACCESSION   DATALS010000448 DATALS010000000
VERSION     DATALS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118470"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..74
                     /locus_tag="VHS06_09850"
     tRNA            1..74
                     /locus_tag="VHS06_09850"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Val,seq:cac)
     gene            922..1335
                     /locus_tag="VHS06_09855"
     CDS             922..1335
                     /locus_tag="VHS06_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988461.1"
                     /translation="MRKFMMRTAPATPGTQVGKVWRQEGSSGLQAGWGVAVGRAPLEG
                     HASGVTALGHLRPRVQARPGLMDGGSTIGPEGWLWWARRVDVLAGWAWPERFAAGDRL
                     GWYTVMKWRYLASYPLTPFSSATRKVGSWDGILYG"
     gene            1348..1932
                     /locus_tag="VHS06_09860"
     CDS             1348..1932
                     /locus_tag="VHS06_09860"
                     /inference="COORDINATES: protein motif:HMM:NF016932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEX2988462.1"
                     /translation="MDTRYSQQYDEDLGRHYSAFWGPRTTRHVWARGSALPQECSVLE
                     YAPLHDIGCWVYATCGMTLHTEARALELFLLSPYQTEGHVELLTAIGHYHQTDARLGL
                     GHTVSFGRPWFEGSRCSFGLVSLPYTFGPELEIACIQQKHVRVLWLLPITEEERAFKM
                     ASGLEALEGLFEERQFNYLDPQRVSVVGTARPSP"
     gene            2068..2697
                     /locus_tag="VHS06_09865"
     CDS             2068..2697
                     /locus_tag="VHS06_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988463.1"
                     /translation="MDDLSPHLADWLPLAPNGDPAKWWFDPPHLRIAEGAWVFTDNEL
                     RGEVGGMSWDNYILSAQVLIHRWAEDAHFQVELTAVGTNVYCQLLPPCIVLAYFSQEK
                     HGTDFFGKSPPLTPEHGVWRELAMQAQNGVITVFFDGRPVLSGKSPHGTQGMPGIVIK
                     KLRHVEIRLKDLRVRFLDPTPAQLAEYQLDALTNWRNHEAAQGAAPQRS"
     gene            2776..3132
                     /locus_tag="VHS06_09870"
     CDS             2776..3132
                     /locus_tag="VHS06_09870"
                     /inference="COORDINATES: protein motif:HMM:NF026924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm8 family immunity protein"
                     /protein_id="HEX2988464.1"
                     /translation="MKPEIKGIFSDDIHSLDREGWIPDDPKQIYIPLTLSIGKVGEAA
                     ADLFSVVVATPSAIQGRPERRGSKLLVVQEYDWPEIRATLQRWVAQCEGPSWEAIVDC
                     LRRRFDWEYEQMAPPQ"
     gene            complement(3199..3348)
                     /locus_tag="VHS06_09875"
     CDS             complement(3199..3348)
                     /locus_tag="VHS06_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988465.1"
                     /translation="MGSICRMPRGGVVEKCGMEFQRTVSEKWEKQEAPVELAVYEIER
                     TKWEK"
     gene            3423..>3530
                     /locus_tag="VHS06_09880"
     CDS             3423..>3530
                     /locus_tag="VHS06_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2988466.1"
                     /translation="MDYALARECWGKGLMTEACQAVLDWGFRAHPSLQRV"
BASE COUNT          673 a          995 c         1157 g          705 t
ORIGIN      
        1 gggcgattag ttcagctggc tagaacgcac ggatcacacc cgtgaggtca caggttcgag
       61 tcctgtatcg cccattgaag cctgaagtgc cgggagcggc gcttcaggct tctttgtttg
      121 gtggcggggc tggtcttgtc cggccccacc ggccggcatg gtcggcgacg ggccccacgt
      181 ggatgtgagc agtgtcgtgt cgctgtcgct ggccaccggt ccaggggccg gattcgcacg
      241 ggctggatgc gaaagcagat gcgcaggttg tggaggggtg ggggataggt gcgtaagttg
      301 ctggtaagga tggggttagt cgttgtttgg gggagcaggt gcgcacgttg catgtgtggt
      361 gcttggtact tggttcttgg tgcttgagga agaccaagat gctcaggtat gcgagtacgc
      421 accctcaccc ccccagcccc ctctcccaga ggtagaggtg gaagaggaag gggcagacca
      481 aggtgcgcaa gttagcgtgc gcatcttcgg cctgggccgc gaggtgcatt acgccggagg
      541 cgagtgacgt gcaacatgtt gcaccaatgc tgcaggggtg aagggcagtt ctgagcggta
      601 gctaccgggg tgcagcttgg gtcatgcaga gccgatgttg catgcagtgc tgcgttgcat
      661 gttgcatccg atgttgcatg cagcggagtg gattcggtgc agtatggcgg tattcggttg
      721 agaaagagcg agcctttccg gcaatgcaac atggcaaatg accaatgcca aatggcagag
      781 caggagcggg cctggagggg cggaaatgcg tccctctatc tatagggggc gttcgcaaga
      841 agtggcaaga gggagatgag gaatggcaag gggttaacga aaaccgaaat tctgcgcgga
      901 ttctataggg agggaagagc agtgcggaag ttcatgatgc ggactgcgcc cgcaactcca
      961 ggcacgcagg tgggcaaggt atggaggcag gaagggagtt caggtctgca ggccgggtgg
     1021 ggcgtggcgg tgggaagagc ccccctagag ggacacgcct cgggcgtgac ggctttgggc
     1081 cacctccggc cgcgggtcca ggcccgacct ggcctgatgg atggcgggag tacgataggg
     1141 ccggaaggtt ggctgtggtg ggccaggcgc gttgatgtgt tggcgggctg ggcatggccg
     1201 gagaggtttg cggctgggga ccggttgggt tggtacacgg tgatgaagtg gcggtatctg
     1261 gcgtcatatc ccttgacccc cttctcctcc gccactcgca aggtggggag ttgggacggt
     1321 attctctacg gatgaggaga ttccgttttg gataccaggt acagccagca gtacgacgag
     1381 gatcttggcc gacactactc ggccttctgg ggaccgcgca ccacgaggca tgtctgggct
     1441 cgaggatctg cgctacctca ggagtgctct gtactggaat acgctccact gcacgatatc
     1501 ggctgttggg tctacgcaac gtgcggcatg acgttgcata ccgaagccag agcacttgag
     1561 ctctttctgc tgtcgccgta ccagacggag gggcatgtgg agttgctaac ggcgatcggg
     1621 cactatcacc agacagatgc acggctcggc ctcggccata cggtgagctt cgggcgtcca
     1681 tggttcgaag gatccaggtg cagcttcggg ctggtctcgt tgccgtatac attcggcccc
     1741 gaactggaga tcgcctgcat tcagcagaag cacgttcgag tgctgtggct actgccgatt
     1801 acggaggagg aacgcgcatt caagatggca tcgggcctgg aggcccttga gggcctcttc
     1861 gaggagcggc agttcaacta cctcgatcca caaagggtct cggttgttgg aacggctcgt
     1921 ccgtcgccct agtgtatgtt aggagggagg ctaaagcccg ttctggcgtc gagcagcatt
     1981 gggaagacgt agcctgctag ggttgcagag cgttgaatcc agtgcgggca cacgccactg
     2041 gcagcatgtg gtgtacactg cgagcgtatg gacgacctat cccctcatct tgcggactgg
     2101 ctgcccctgg cgcccaacgg cgaccccgcc aaatggtggt tcgatccccc acacctgcgg
     2161 atcgccgagg gcgcgtgggt cttcaccgac aacgaactcc gcggcgaagt cggcggaatg
     2221 tcctgggaca actacatcct ctccgcccag gtcctgatcc atcgctgggc cgaagatgct
     2281 cacttccagg tcgagctgac cgccgtcggc acgaacgtct actgccagct cctccctccc
     2341 tgcatcgtcc tggcctactt ctcccaagag aagcacggaa ccgatttctt cggcaaatcc
     2401 ccgcccctaa cgcccgagca tggcgtctgg cgggaactgg ccatgcaggc acagaacggc
     2461 gtgatcaccg tgttcttcga cggccgcccg gtcctcagcg gcaagagccc ccatggcacc
     2521 caaggcatgc ctgggatcgt catcaagaaa ctccgccacg tcgagatccg cctgaaagac
     2581 ctccgcgtcc gcttcctcga ccccaccccc gcgcagctgg ccgagtacca gcttgacgcc
     2641 ctgaccaact ggcgcaacca cgaagccgca caaggagccg ctccccagcg cagttgagcc
     2701 cattctgctg gagctgtgag gtggagctaa cccgctagcg ttgcagagca ttcaaccaag
     2761 tacggagcag cagtaatgaa gcctgagatc aagggcatct tttccgacga tatccatagc
     2821 ttggatcgcg agggatggat accggacgat ccaaagcaga tctacatccc actcacacta
     2881 tcaatcggca aggttgggga agcggcagcc gatctgttct cggttgttgt ggccacaccg
     2941 tccgctatcc aggggcggcc cgaacgcaga ggctccaagt tgttggtggt tcaggaatac
     3001 gattggccgg agattcgggc aacactccag agatgggttg cgcagtgtga agggccgagt
     3061 tgggaggcaa tagtggattg ccttcgccgc cgatttgact gggagtacga gcagatggct
     3121 cctccgcaat gacgactgcc tctgctcggg gtgcaacgtg ttaacgctgc aaagcattca
     3181 accgtgtgcg ggttcccttc acttctccca cttcgtccgt tcgatctcgt agaccgccaa
     3241 ctccaccggt gcctcctgct tttcccactt ctccgacacc gtcctctgaa actccatccc
     3301 gcacttctcc accacgcctc ctcgcggcat tcggcagatg cttcccatcc tgatcttctc
     3361 tcgctccaac agtggctcga tgctctgacc gctatggcgt gggcgattga aagaccggat
     3421 cgctggacta tgccctggcg cgagagtgtt ggggcaaggg gttgatgacc gaggcatgcc
     3481 aggccgtgct cgactgggga ttccgtgccc atccgagcct tcagagggtc
//
LOCUS       DATALS010000449         5359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_118687, whole genome shotgun sequence.
ACCESSION   DATALS010000449 DATALS010000000
VERSION     DATALS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118687"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VHS06_09885"
     CDS             complement(<1..456)
                     /locus_tag="VHS06_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX2988467.1"
                     /translation="MAKYVGAVDQGTTSTRFMIFDHSGGVVGVHQLEHQQIYPQPGWV
                     EHDAMEIWARTQEVIRGAMEKTGISAADLAAIGVTNQRETTVVWNPKTGKPYYNAIVW
                     QDTRTDKIINAFAQEGGQDRFRAKVGLPLATYFSGPKIKWILDNVAGVRE"
     gene            complement(552..1367)
                     /locus_tag="VHS06_09890"
     CDS             complement(552..1367)
                     /locus_tag="VHS06_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX2988468.1"
                     /translation="MLWELAAEVLGTFILILLGDGVVANVGLAPRLAAGGYNWNTITI
                     GWCFAVVIAAYATGGVSAHLNPAVTLAMAVKRNFPWSKVIPFWVAQVVGAFLGALAVF
                     LCYRDGLVAAGMPNVWTTGPGSVFGQAFWGGGAGQAAGQYALITAVITEIFGTAILLF
                     GICSADKVTNNLGTLLVGFAVLAAGLSLGGPSGYSLNPARDFGPRLLGVLVGTQGLFD
                     GIYWLVAPVIAPFIGAVGGIYVYDWVVTAGLAAKKAEEAAVAKGKAGRPAAQA"
     gene            complement(1712..3205)
                     /gene="glpK"
                     /locus_tag="VHS06_09895"
     CDS             complement(1712..3205)
                     /gene="glpK"
                     /locus_tag="VHS06_09895"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX2988469.1"
                     /translation="MARYILGVNQRTAGTIAAIFDRRGRVVAKAQGDVPQYSPQPGWV
                     EYDANDLWQVTLKVIAEAFRNGRIAPDDIDAIGIANQRDTTVLWNKKTGEPVGRAIAW
                     QDQRTLPLCEYLITKYRAEIEDRTGMIIVPDVTASKIRWLMDHDKVVQKGVARGELIC
                     GTVDSWLIWKLSGGGVHVTDYSNASLTLMQDARTLTYDPWILDELGIPREILPELHAS
                     SEIYAYTAPEAFFDARVPIAGAAANQQVAALGLACLAPGTAKTTYSSGSFLILNTGSS
                     YRPPVGGLFSPALWVRDDEVVYGLEGKADVSGTAIQWLRDGLGLIRESGEAEGLAAQV
                     PDTQGVYFVPAFVGLGAPHYDRYARGTMMGITNNTTKHHVARAALEAIAYQTRDAFEI
                     MRRESGFELKMMRVDGPGAKSDFVMQFQADILGIPLERPAMTEAAVLGAAYLAGLAVG
                     YWQTTDEIAANWQLDCRFEPRISLERCEELYYGWQKAIEHAGGWLRR"
     gene            complement(3220..3879)
                     /locus_tag="VHS06_09900"
     CDS             complement(3220..3879)
                     /locus_tag="VHS06_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2988470.1"
                     /translation="MIRVVIAEDHHIVRQGICKLLEEDGTIQVVGEADNGRDALAMIK
                     RLQPDVLILDIAMPQLNGLEVLPQLRTCRPSPRVVVLSMYGDLATVQQALQGGALGYV
                     LKQSVATELLSAVRVVHGGSVFLSSGVSQVLSSDFFAPRPRNLLDRLSPREREVVRMV
                     VEGHSTREIADFLSTSVKTVEKQRRDAMRKLGVDNLASLVRVCLELGLITGAEARPKA
                     L"
     gene            complement(3970..4905)
                     /locus_tag="VHS06_09905"
     CDS             complement(3970..4905)
                     /locus_tag="VHS06_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX2988471.1"
                     /translation="MNETIAQATGHSGTELIGSNVWDLYPADKAKHRRIIVHRVVETG
                     QPMVFVDRDGDQWYETLVQPLWDDGQIRHLALYARNITLQIRAEERLKHTSLQLVTAE
                     EDERRRIAQDLHDDIGQRMTALTLSLKAIQSAVTEGDKGAVEQLKDAIRMAESIMRQV
                     RQILYQLRPPLLATLPLAKVLEELCSSIAQSSRLSVDFSSEPDVPAVPEIQAMAFYRC
                     VQEGLNNVVRHAKATSVWVNLDCVDGELSVSLEDDGQGFEPDYEQKLGLGLQGIRDRF
                     LMLGGTFSIESKPGCGTRLFGSVPLAGATTPYVGR"
     gene            4945..5184
                     /locus_tag="VHS06_09910"
                     /pseudo
     CDS             4945..5184
                     /locus_tag="VHS06_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
BASE COUNT         1116 a         1655 c         1546 g         1042 t
ORIGIN      
        1 ctcgcgcacc cccgccacgt tgtccaggat ccacttgatc ttgggccccg agaagtaggt
       61 cgccaggggc aagccaacct tggcccggaa gcggtcctgc ccgccctcct gggcgaaggc
      121 gttgatgatc ttgtccgtgc gggtgtcctg ccagacgatg gcgttgtagt acggcttgcc
      181 ggtcttgggg ttccagacca cggtcgtctc ccgctggttg gtgaccccga tcgccgccag
      241 atctgccgca ctgatcccgg tcttctccat cgctccccgg atcacctcct gggtgcgggc
      301 ccagatctcc atcgcatcat gttcgaccca gcccggctgg gggtagatct gctgatgctc
      361 gagctgatgc accccgacca ccccgccgct gtgatcgaag atcatgaagc gggtgctggt
      421 ggtgccctga tcgactgccc ctacgtactt tgccatggaa gtcgctccta tgcagatgct
      481 tgcagaagcc cggttctcac ccgagagacc gggcttctca gtaccaatga ccgaaaatgg
      541 gacgaggact atcaggcctg tgcggccggt ctgccagcct tgcccttggc tacagcggct
      601 tcttcagcct tcttggcggc caggccggcg gtcactaccc agtcatacac gtaaatgcct
      661 ccaacagcgc cgatgaaggg tgcgatgact ggcgccacca accagtagat gccgtcgaac
      721 aggccttgtg tgccgaccag cacgcccagc aggcgagggc cgaaatcacg ggcggggttc
      781 aacgagtagc cactcggacc accgagagag aggccggcgg ccaggacggc gaaaccaacc
      841 agcagggtac cgaggttgtt cgtgacctta tcagcagagc agatgccgaa caagaggatt
      901 gccgtgccaa agatttcagt gatcactgcc gtaatcagag cgtactgccc agccgcctgt
      961 ccagcgccac cgccccagaa agcctgtccg aagaccgaac cgggccccgt ggtccagaca
     1021 ttgggcatgc cagcagcaac cagaccgtca cggtagcaga ggaagactgc gagtgcgccg
     1081 aggaaagcgc ctacgacctg ggcgacccag aacgggataa ccttgctcca ggggaagttg
     1141 cgcttaacgg ccatcgccag ggtcaccgcg ggattgaggt gagcagagac gccacccgta
     1201 gcataggcag ctatcaccac cgcgaaacac cagccgatcg ttatggtgtt ccagttgtaa
     1261 ccacccgctg ccagacgggg agccaacccc acattcgcaa caacgccatc acccagaaga
     1321 atcaggatga aagtgcccaa gacctcagcg gcgagttccc agagcatatt gttgctcctt
     1381 tcagtgtaca ggttcacccg attcgtaccc gaactacaac ctccaggttt tcggcaacca
     1441 ccctccttcc tttgggaaca atcgcaaagg ataaacaatg tagggattat tacacggcga
     1501 ggcgagcttg agaatcagga ttcacaccca gttgaggggg aagatggcgt atggcagggg
     1561 tccaagaggc gcatgaccta tacgcctttg gagtacgtac aggtcatgcg ccagaatctg
     1621 ggtgattcgc accggattag tgctctgcct ggagattaag agtctacggg ttcggaaatg
     1681 ggggagatga gggaagggct ctccaatttc gttatcgtcg caaccaccca ccggcatgct
     1741 cgatcgcctt ttgccagcca tagtagagtt cctcgcatcg ctcgagggag atgcgaggct
     1801 caaaacggca gtcgagttgc cagttggccg cgatctcgtc agtggtctgc caatagccaa
     1861 ccgccagccc agccaggtag gcggcaccga gcacggctgc ctcggtcatc gcaggacgct
     1921 ccaaagggat ccccaggata tcggcctgaa actgcatcac gaagtcgctt ttggcgcccg
     1981 gaccatcgac acgcatcatt ttcaactcaa agcccgattc gcgccgcatg atctcgaaag
     2041 cgtctcgggt ctggtaggcg atcgcttcca gagcggcgcg cgccacgtgg tgcttcgttg
     2101 tattgttcgt gatacccatc atagtgccgc gcgcatagcg gtcataatgc ggggcaccga
     2161 ggccaacgaa agccggcaca aagtacacgc cctgggtgtc cggcacctgg gcagccagcc
     2221 cctcagcttc gccggactcg cgaatcaatc ctagtccgtc ccgtagccac tggatggccg
     2281 tgcccgacac gtcggccttg ccttccaggc cgtagaccac ctcatcatcg cgcacccata
     2341 gagcggggga gaacagccca cccacaggcg gtcgatacga gctgccggtg ttgaggatca
     2401 agaatgagcc actgctgtag gtcgtcttgg ctgtgccggg cgccaggcag gccaggccaa
     2461 gcgcggcaac ctgctggtta gccgctgccc cggcgatcgg gacccgggca tcgaagaacg
     2521 cctcgggggc agtataggca tagatttcgc tggaagcatg caattcgggc agaatctcac
     2581 gcgggatgcc cagttcgtcc aggatccatg gatcataggt cagcgtacgg gcatcctgca
     2641 tcagcgtcag cgatgcgttg gaatagtcgg tcacgtggac cccgccgccg gacagtttcc
     2701 agatcagcca gctatccaca gtgccgcaga tcagttcgcc tctggcaacc cctttctgca
     2761 cgaccttgtc atgatccatc aaccaccgga tcttgctagc cgtcacatcc gggacgatga
     2821 tcatgccggt gcggtcttca atctctgccc ggtacttcgt gatcaggtac tcgcacagtg
     2881 gcagggtccg ttggtcctgc caggcgatcg ctctgccgac cggctcgccg gtcttcttgt
     2941 tccagaggac ggtggtatcg cgctgattgg cgatcccgat ggcatcgata tcgtctggcg
     3001 cgatccgccc attgcggaag gcctcggcga tcaccttcag ggttacctgc caaagatcgt
     3061 tagcatcgta ctccacccaa ccgggctggg gcgagtactg cggcacatcc ccctgcgctt
     3121 tggcgacaac gcgtcctctg cgatcaaaaa tggcagcgat cgttcccgcc gtccgctggt
     3181 tcaccccaag aatgtatctc gccatcctgc tcccttcctt cacagagctt tcggacgggc
     3241 ctctgcgccc gtgatcaggc caagttccag acagacgcgt accaggctgg ccaggttatc
     3301 aacgccgagt ttgcgcatcg catcccggcg ctgtttctcc accgtcttga cgctcgtgga
     3361 caggaaatcg gcaatctcgc gtgtcgaatg gccctctacc accatgcgca cgacctcacg
     3421 ctcacggggt gacaatcgat cgagcaagtt gcgtgggcgg ggtgcaaaga aatcactgct
     3481 taacacttgg gacacgcctg agctcaggaa cacactgccg ccgtgcacca cacgcactgc
     3541 agacagcagc tccgtagcca cagactgctt cagtacatat cccagcgcgc cgccttgcag
     3601 tgcctgctga acagtagcca gatcgccgta catcgacagc acgacgacac gtggcgacgg
     3661 ccggcaggtc cggagttgag ggaggacctc cagtccattg agttgaggca tggcgatatc
     3721 gaggatcaag acatcaggct gtaggcgctt gatcatggcc agagcatcac gcccattgtc
     3781 ggcttcaccg accacctgaa tcgtgccgtc ctcttccaga agcttgcaga tcccctgccg
     3841 tacgatatga tgatcttcgg cgatcacaac gcgtatcata gtcaagcctc attgctagaa
     3901 ccgatgacgc gagatacaaa attctccgtg gtccccgtgt ctccgtggtg caaccgaggt
     3961 tgtcagacac taacggccca catatggagt agttgcgccg gccaacggca ccgagccaaa
     4021 cagacgagtt ccacagccag gttttgactc gatgctgaag gtgccgccaa gcatcagaaa
     4081 gcggtcccgg atgccctgca aaccgaggcc aagcttctgc tcataatcag gctcaaatcc
     4141 ctgtccgtcg tcctccagcg acacgctcag ttcaccatcc acacagtcca ggttcaccca
     4201 aactgaagtt gccttggcgt gtcggaccac attgttcaag ccttcctgga cgcagcgata
     4261 gaacgccatg gcctgtatct cggggactgc cggcacatca ggctcgctgg agaaatccac
     4321 actcaaccgg ctcgactgag caatggaaga acacagttcc tccagcacct tagcgagagg
     4381 taatgtggcc aacaacggtg ggcggagttg atacaggatc tgtcgtacct gacgcatgat
     4441 gctctcggcc atccggatcg cgtctttcag ctgctccaca gcccctttgt caccctcggt
     4501 cacggcgctt tggatcgcct tgaggctaag cgtcaaggcg gtcatccgct ggccgatatc
     4561 atcatgtaag tcttgggcga tccgccgccg ctcatcttcc tcggccgtga cgagttggag
     4621 gctggtatgc ttcagccgtt cttcagctcg gatttggagg gtgatattgc gagcgtaaag
     4681 ggcgaggtgt ctgatttgcc catcatccca aagcggttgg acgagcgtct cataccactg
     4741 atcgccatca cgatcgacga agaccatagg ctggccggtc tcgaccactc ggtgaacgat
     4801 aatccgtcgg tgcttggcct tgtcggccgg atacaggtcc cagacgttag acccgatcaa
     4861 ctcggtgccg gagtgaccgg tggcttgagc gatggtctcg ttcacaaaga cgatggaccc
     4921 gtcgggagtg atcagggcaa tcccatcgac cgcgaggggc tataccagca tggtgccctg
     4981 agtcagggga tggggtggca agtgccgaga ctgccgggcc tcggcgacat cgactgggcg
     5041 aagttcatca gcgcactgta ccacgtcggg tacgactacg tggtgagcat cgagcacgaa
     5101 gaccgggcgt ttgagaagac cgaggacctc gtcagacgag ggttcctgct gtcgcgcgat
     5161 gtcctgcggc cttacataaa gtagtgatgc gtttttgaca tatttggccg cgctgtccaa
     5221 ttgcgtcggc ggatggccta tggtagaatt gacttgaacc accatgtatt tgcacagcct
     5281 atgtttgtgc cggcccgcgc cggttggcgg aagggtcggc ataacaccgg gcctcatctc
     5341 gaaaaaagga ctccgctgt
//
LOCUS       DATALS010000450         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_70929,
            whole genome shotgun sequence.
ACCESSION   DATALS010000450 DATALS010000000
VERSION     DATALS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70929"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VHS06_09915"
     CDS             <1..314
                     /locus_tag="VHS06_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004124874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2988472.1"
                     /translation="TLLLENEELCVCDFVGALGQTQSKISRHLRYLYNAGLVEDRREG
                     LWMHYRLSSKLTPEQAAIVQTLSEVVGDEQKAELRQALEQWLGGKTAEVLCGAAEASC
                     G"
     gene            351..608
                     /locus_tag="VHS06_09920"
     CDS             351..608
                     /locus_tag="VHS06_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2988473.1"
                     /translation="MIEVKVFGAEPPCVKCTQSEQEARKAAVKFPGQVTVEKLSALSA
                     EGVAFGFTMTPAVVVNGRVVSQGRVPQKDELERIFQSELGG"
     gene            610..885
                     /locus_tag="VHS06_09925"
     CDS             610..885
                     /locus_tag="VHS06_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2988474.1"
                     /translation="MAVLLEVFSGDPPCPGCVAILALADRLAEKYPAEELEVRKYVGK
                     EALAKYGEYHLSCVPAAVINGVIKIEGTAPDDGTMNNALREGGLWIR"
     gene            873..1136
                     /locus_tag="VHS06_09930"
     CDS             873..1136
                     /locus_tag="VHS06_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988475.1"
                     /translation="MDPIKVEVFLSVPACSGGLAVRRLIEDVQKQYGSQMEVIYHQGR
                     DERYEELKLSSAPAIIVGDLVRFIGLCPTREGLIGALREAGLE"
     gene            complement(1187..1645)
                     /locus_tag="VHS06_09935"
     CDS             complement(1187..1645)
                     /locus_tag="VHS06_09935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988476.1"
                     /translation="MAVAGPATVAGTHLQVLKLRSDLKEVAQVRALIAQAGAREGLSL
                     DLIFDMQVAASEACANAIEHANSEVQVLLWWMVSQVVAEIKNVGKFRPRPRTANECSR
                     GMGVGLMVSLADKVEIAGKDPERTTIRLWFNRRGVSPDPAEVEGLELGRS"
     gene            1834..2202
                     /locus_tag="VHS06_09940"
     CDS             1834..2202
                     /locus_tag="VHS06_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988477.1"
                     /translation="MYVLAYVGAALTVIWGVAHLLATRGVVADFEQVTIDNRRIVTME
                     WIVEGVTLVFAGALAAAVTAIDPQNAVSATAYALVIALLVVLTGVSLATGFRIRFLPF
                     RLCPVIFMLSAVLIAIGAWL"
     gene            2209..2739
                     /locus_tag="VHS06_09945"
     CDS             2209..2739
                     /locus_tag="VHS06_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006032713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2988478.1"
                     /translation="MTADPLLVRFSSLLEDAALDGPVLDLACGTGENGLFVAGLGVPV
                     ILADRSAQALAVARSSAQVQGIEADFWEVDLETGANPLQEEHYRATLVFHYLFRPLIL
                     CIRKGVRAGGLLIYETFTSEQPTYGRPHNPDYLLQPGELAGWFEDWQLVHYFEGVLDD
                     PRRALAQIVCRKPRQS"
BASE COUNT          543 a          873 c          940 g          552 t
ORIGIN      
        1 tgacgctgct cttggagaac gaagaactgt gcgtctgcga cttcgtcggt gccctcgggc
       61 agacacagtc caagatctcg cggcacctgc gctacctcta caatgccggc ttggttgaag
      121 accgtcgtga gggactgtgg atgcactatc ggctctcgag caagctgact cctgagcagg
      181 ccgctatcgt gcagacgctc agcgaggttg tgggcgacga gcagaaggcg gagcttcgac
      241 aagccttgga gcagtggctc gggggcaaga cggctgaagt gctgtgcggc gcagcagagg
      301 cttcgtgtgg ttagttccac tcgaccacga gtgattcgaa agggggagct gtgatagagg
      361 tcaaggtgtt cggcgctgag ccgccgtgcg tcaagtgcac gcagagcgag caggaggcga
      421 ggaaggctgc ggtcaaattc cccgggcagg tcaccgtcga gaagctctct gccctctcgg
      481 ccgagggtgt cgcgttcgga ttcaccatga ctccggccgt ggtagtcaat ggccgggtgg
      541 tctcgcaagg ccgggtgcct cagaaagacg agctggaacg catctttcaa tcggaactag
      601 gagggtgaag tggcagtgct cttggaagtc ttcagcgggg atccgccttg cccaggctgc
      661 gttgccatcc tcgccctggc cgaccggctc gcggaaaaat acccagcgga agaactcgag
      721 gtgaggaagt acgtcggcaa ggaagccctg gcaaagtatg gcgagtacca cctgtcctgt
      781 gttccggccg ccgtcatcaa tggtgtgatc aagatcgaag gcacggcgcc ggatgacggc
      841 actatgaaca atgctctgcg ggagggcggg ctgtggatcc gataaaggtg gaggtctttc
      901 tgtcggtgcc cgcctgttcg ggtggcctgg cggtgaggcg gctgatcgaa gacgtgcaga
      961 agcagtacgg aagccagatg gaggtcatct accatcaagg ccgcgacgag cgatacgagg
     1021 agctcaagct atcgtccgcc ccggcgatca tcgtgggcga cctggtccgc ttcatcggcc
     1081 tctgcccaac cagagaggga ttgatcgggg cactgcggga ggcggggctg gagtagccgg
     1141 cgttggagga ggctgcccag gactgggccg gggaggccca ccgaggctag gacctcccga
     1201 gttcgaggcc ttccacttcg gcaggatcag gcgagacgcc acgccggttg aaccacagtc
     1261 tgatggtcgt tctctctgga tccttgccgg cgatctcgac cttgtcggct agagacacca
     1321 tgagtcccac gcccatgccc cggctgcact cattcgcggt tcggggacgc ggcctgaact
     1381 tgccgacgtt cttgatctcc gccaccacct ggctaaccat ccaccagagg agcacttgga
     1441 cttcggagtt cgcgtgttcg atagcgttcg cacacgcttc ggacgctgct acctgcatgt
     1501 cgaagatcag atcgagggag aggccttcgc gagcgcccgc ctgggcgata agcgcccgca
     1561 cttgtgcgac ctccttgaga tcgctgcgca atttgaggac ctgcagatgt gtgcctgcga
     1621 cggtcgccgg cccggccaca gccatcccga cctctctaac gtagaaacac tagaccacgt
     1681 caccgcagct tgtaccccaa cgcaaagcgt ggcaatcacg cggcaacgca aagtgctaat
     1741 ctgccgagac ggcccgccgg cagagtgacc gggtgacggg ctgggcagcc ccggtcttgc
     1801 cgggcggcgc agcaaaagaa ggagtcccct cgcatgtatg tgctggccta cgtaggagcg
     1861 gccctcacgg tcatttgggg agtcgcccac ttgctcgcga cccggggcgt cgtggcagac
     1921 ttcgaacagg tgaccatcga caaccgccgg atagtcacca tggaatggat cgtcgaggga
     1981 gtcacgcttg tcttcgcggg ggccttggcc gccgccgtga ccgccatcga ccctcagaat
     2041 gccgtctccg ccacagccta tgcacttgtg atcgcattgc tcgtcgttct gaccggggtg
     2101 tcgctggcaa ccgggttcag gataagattc ttgcccttcc ggctctgccc ggtcatcttc
     2161 atgctctcgg ctgtactgat tgcgatcggc gcttggctgt gacgaagaat gactgctgat
     2221 cccctactgg taaggttcag ctctctgctg gaggacgccg ccctggacgg ccccgtcctc
     2281 gatctggcat gtggtacggg agaaaacggt cttttcgtag ccggcttggg cgtgccggtc
     2341 atcctcgccg atcgatccgc gcaggctctc gcggtcgccc ggagctcggc ccaggtacag
     2401 gggatcgagg cggacttctg ggaggtcgat ctcgagaccg gtgcgaaccc gcttcaggag
     2461 gagcactacc gggcgactct cgtcttccat tacctctttc gcccgcttat cctttgcatt
     2521 cgaaaaggcg ttcgcgccgg cgggctcctc atctatgaga ccttcaccag cgaacagccg
     2581 acatacggcc ggcctcacaa tccggactac ctgctgcagc cgggagaact ggccggttgg
     2641 ttcgaggatt ggcagctcgt ccactacttt gaaggcgtgc tagacgaccc gcgacgggcg
     2701 ttggctcaga tcgtctgcag gaagccgcgg cagtcataga aagagcccta gagacccacc
     2761 gggtcttgcc ttggcgattc cgggtctttc ggtcgcccaa agccttacac gtgttcgctg
     2821 cagtcaccgc gccggttaca atctcgtcgc cgtagcaacc agcgtcggga tccagcgaac
     2881 aaggaacagg ccatgccaga taggaaga
//
LOCUS       DATALS010000451         8494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_348339, whole genome shotgun sequence.
ACCESSION   DATALS010000451 DATALS010000000
VERSION     DATALS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348339"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VHS06_09950"
     CDS             <1..687
                     /locus_tag="VHS06_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX2988479.1"
                     /translation="IFMTFSGTTPGTFTLAHELGHAYHNSVQRDLPAMARGFSMCTAE
                     TASTFAEGVVSRAALAGATTKEERLALLDGAIHNVVAMFMNIHARFLFEERLYEARQK
                     GLLGVEQLNGMMEEAQREAYRDGLVAYHPQFWASKGHFYGTGTAFYNFPYTFGFLFSS
                     GIYARAVSEGPGFADKYVGLLRDTGRMDTEELAGRHLGVSLETPEFWNEAVRVAMEPV
                     EEFIAAASQR"
     gene            complement(829..2277)
                     /locus_tag="VHS06_09955"
     CDS             complement(829..2277)
                     /locus_tag="VHS06_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988480.1"
                     /translation="MTRAITLFFATLVICLALFSHSAVASREFPETGYSITNSKILDY
                     YDHRGGARVFGSPISREFQLAGTQVQLFQKAAIQIQANGGAGLLDIMNESWFPFTHYN
                     GSTFPAVDPHMIAVAPSPIDPDYAERSVDFARYFAPDLWRGEETNFFKTMFGTVTYEE
                     AFPDGETSADLLPLVNLEVWGLPISKPAYDPSNGNFLYLRFMRGMMLYDKTTGVTQAL
                     PVGEYFKALLTGQGAPADLARDAQASKLYRQYDNGAMNGLARLAEMPATNIFAAFEKD
                     GVVVPTPMPPTPTPLPPTATPIPTATSVPVAAAATQAPAAAGVPAGANAQPPATITIV
                     GDASFTRRVNDALSLLAEKSPYTYSIVKQNVFRIERSDNSYPTSDLPNRTFKLNDGTI
                     WYSDWGSKPENETQWFASLLVHNAVHVSQYFRGAATTGAEAEKEALMAQRDALAGIET
                     TDPKGQFWTYVNEALNDNTGWFGDWQNPRDPK"
     gene            2499..3587
                     /locus_tag="VHS06_09960"
     CDS             2499..3587
                     /locus_tag="VHS06_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEX2988481.1"
                     /translation="MILAVVAAVAIPLVFLYLVRWLDLYASGSFRLVVLCLVWGCVAF
                     ALAYNINTLFVPLVGYGLIVTLVAPIVEECLKSLVLLYLVRRPQFTYFVDGAIYGFAS
                     GTAFAVLENLLYLSRASADMGIIVVLSRAFSTSLMHGSASAMVGVSLGRFRFARGASR
                     PASLVLGWLAAMAIHITFNHVISGVEGTLALILAVVLGLGGIALTVAFIMWGLAEERK
                     WLQETLGSDAGVSQGESALVQKMADLDLLMAPVEERFGSVRRKQVERFLQLQVQLGLK
                     SKAQTMTQDGRQSESLTGQIEELRTEMDEIRRTVGVYCMSYVRSILPPTTEPVWDRLT
                     QSLEGQNKPAPTRSLWSALGDKMDASDG"
     gene            3580..4860
                     /locus_tag="VHS06_09965"
     CDS             3580..4860
                     /locus_tag="VHS06_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988482.1"
                     /translation="MGSRGCRSRRCAPVHLRIRNLATIVVALAIASFVGVLAPAPSMV
                     ADDGQVEKSVAVARTAPGPESEQDGAMCPSPSVPRISSINGRFLLLNMHGALFHSDIR
                     DLEEDITYARWMNAGAIRVFATDNNTFKNWSGRQVGNRIADAAPTLRANNVKLLVALV
                     NNHKPVPGEAANSFGWMDDFDQLLLPFYTDNWRRDYLPFVRELISTVIARGARDVIFA
                     WEPGNELHTTRNPQAILPFFNQVIEEIKKVDPETPILPGTMGVEHLNPGNPLSPLGPW
                     MYCHAPVDGYTLHVYDWLSPQRQGDSTVANDLNIVLSQPCWNGRRLPVIVEELGTSRT
                     MPGVYSTEEEEKRLQQELNQLRYLLSHPDVKVIGVGPWNAESPKVKEKLFYDDRRGFT
                     SYGPSRDGSGSCYTGESGVRCRLEQLYRNLPALP"
     gene            complement(4902..6356)
                     /locus_tag="VHS06_09970"
     CDS             complement(4902..6356)
                     /locus_tag="VHS06_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988483.1"
                     /translation="MLLLTKDNSRLVLGSRSEGNASFTLAFTYRGIVDGSPRLEVACR
                     CSGRTERATATLEAVADQSRLSAILPSLNGSPARYSLTLVDSGPDHIRLTVATPLAIT
                     CEEGWSGAGLSVLDVLSASEANTQLLSWLLRKGEATLPEIVEQLGMEEKECKTLIARL
                     VSQSQVLEVRPEGGATSYRARLSRRKGRTLSSEVWSALEDAPQAEEGLPEKQHTSALA
                     GIRRRLSALFTGERGRFLISLTPVILAFLFSEWALVTDSSSFTAVLNLLGVISVTIFV
                     GIIPVLLILSSRRKGDMVPGFNYRVLGNPVLQGVIYVIFLAAILLHGLVIWQQPLEKA
                     AALVTTAIVLGMTVTVIRRGALRSRAVIELRQDSGKPSLSVSAILSGIAVEAKVEASG
                     LRETPSLASDRVLPGAGPLFMTVSLPEGGADELKLWAHRINEEGDSEPLAAFAVLNAS
                     GGTEEFDLRMLGGEAVTRLEGPQWDVEIKVHPGA"
     gene            complement(6304..7740)
                     /locus_tag="VHS06_09975"
     CDS             complement(6304..7740)
                     /locus_tag="VHS06_09975"
                     /inference="COORDINATES: protein motif:HMM:NF015197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HEX2988484.1"
                     /translation="MQARRARTLLYLIENRTAYLAARSKRAMALYDSEEGERKQYLDF
                     LESFALGKDPPIRPSIQDIERYAPEWAPLLPDNPALRAALLYALGQKYRFNYRSIPGI
                     RAAAGLDAPEVAAAFSRAHGKSAEEQFSAPVSVRERLGYASSRVSTSLETLPPFWAVF
                     ALTLTETVGSGVLALPIAMAKVGPAAGVALLIVIGLINVITIAAMAETVTRTGSVRYG
                     GSYFGRIVTGYLGTAGASVMTVALASIFFLGLVSYYLGLASTLYSSTGIVPELWAALL
                     FVTAIYFLSRGSLDSTIASALVVGAVNVLLVFALCVLAFSRMNPGLLGYINLPFVDGR
                     PFDPSVLELVFGVVLCTYFGHTSVGNCGKLVLRRDPSGRSLIWGVVCAELAAMLLFCV
                     WVVAVNGAVPTEILISEKGTSLSPLATVVGPVVHPIGSIFVLLAMGMTSIHFSLGLFN
                     LVKERLPRLAPRVAAHERQLTAGAGIAI"
     gene            complement(7858..>8494)
                     /locus_tag="VHS06_09980"
     CDS             complement(7858..>8494)
                     /locus_tag="VHS06_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988485.1"
                     /translation="SFLILSSRRKGDMVPGYRSRLLGSAPLQVAVYLFFVGAILAHGM
                     VIWQDPLLKLAAFVSALLIVVMTVVVLRHGAMRSRAVVELRQEEGEESLKVTAIQAGE
                     YSDACIALAGMRGQPITAVGYAVPTSGLLTLGISMPRWRAAELKVWAHRVKEDGNSEP
                     LPASALLNSSRGAEEVDLRLTGGERLVELDTREWDLQVRVESGAQPFPARR"
BASE COUNT         1662 a         2620 c         2650 g         1562 t
ORIGIN      
        1 atcttcatga cgtttagcgg cacaacgccg ggaaccttca ccctggcgca cgaactggga
       61 cacgcctacc acaactccgt ccagcgcgac cttccggcga tggcaagagg attcagcatg
      121 tgtaccgccg agacggcatc caccttcgca gagggcgtcg tctcccgagc cgcgctggcg
      181 ggagccacca cgaaggagga gcggctcgcg ttgttggacg gtgcgatcca caacgtggtg
      241 gcgatgttca tgaacatcca cgctcgcttc ctcttcgagg aacggctcta cgaggctcgc
      301 cagaaggggt tgctgggagt ggagcagctc aatgggatga tggaggaggc tcagcgggag
      361 gcctacagag acgggctggt ggcctaccac ccgcagttct gggcatcgaa gggccacttc
      421 tacggcaccg gcaccgcgtt ctacaacttc ccctacacat tcggcttcct gttcagttcc
      481 gggatctacg cgcgagcggt atcggaaggc cccgggttcg ccgacaagta cgtcggactc
      541 ctcagggaca ccgggcggat ggatacggag gaacttgccg gccggcatct gggagtcagc
      601 ctcgaaacgc cggagttctg gaacgaggcc gtgcgcgtag cgatggagcc agtggaggag
      661 ttcattgccg ccgcgagcca acggtaggac cgcggctccg gccggtcaca tcctggtgta
      721 gcagcggtcg agggctctgg cgcggtttat ccaggtaagc gatcgagggc tccagacgat
      781 tgtccggagc cctcgactct tccgttggct agatgcggtt tgacgggatt acttgggatc
      841 tcgcgggttc tgccagtcgc caaaccagcc ggtgttatcg ttgagcgcct cgttgacgta
      901 ggtccagaac tgccccttcg gatcggtcgt ctcgatgccg gccagagcgt ctctctgcgc
      961 catcagcgcc tctttctcgg cctccgctcc ggtggtggcg gctcccctga agtactggga
     1021 gacgtgaacc gcgttgtgga ccagcaggct cgcaaaccac tgcgtctcgt tctctggctt
     1081 gctgccccag tcggaatacc agatcgtgcc gtcattcaac ttgaaggtcc gatttggcag
     1141 gtcggaggtg ggatagctgt tgtccgacct ctcgatcctg aagacgttct gcttgacgat
     1201 cgaataggta taggggctct tctcggccag cagcgagaga gcgtcgttta ccctcctcgt
     1261 gaaggacgca tcgcccacta tggtgatcgt tgcgggaggt tgggcgttgg caccggccgg
     1321 gacacccgcg gccgcggggg cctgagtcgc cgcagccgct acgggcacgg aggtagccgt
     1381 tgggatgggc gtagcggttg gcggcagcgg tgtcggtgtc gggggcatcg gtgtgggcac
     1441 caccaccccg tccttctcga atgccgcgaa gatgttggtc gccggcatct cagccagccg
     1501 cgccagcccg ttcatcgcgc cgttgtcgta ctgccggtac agcttgcttg cctgtgcgtc
     1561 tcgagccaga tcggccgggg caccctgccc cgtcaggagt gccttgaagt actctccgac
     1621 aggcagcgcc tgggtgacgc cggtcgtctt gtcgtagagc atcattcctc tcatgaagcg
     1681 cagatagagg aagttgccgt tactgggatc gtaggccggc ttgctgatcg gaagccccca
     1741 gacctccaga ttgaccagag ggagcaggtc ggctgaggtc tcgccatcgg ggaacgcttc
     1801 ctcgtaggtg acggtgccga acatggtctt gaagaagttg gtctcctcac ctcgccacag
     1861 atcgggcgcg aagtagcgtg cgaaatccac cgacctctcg gcgtagtcgg gatcgatggg
     1921 cgacggtgcc accgcgatca tgtggggatc gacggcaggg aaggtggagc cgttgtagtg
     1981 tgtgaacggg aaccagctct cgttcatgat gtcgagaagt ccagcgcccc cgtttgcctg
     2041 gatctgaatg gcagccttct ggaatagctg aacctgggtt ccggccaact ggaactcgcg
     2101 agagatcggt gaaccaaaca ccctggcacc gcctctatga tcgtagtagt cgaggatctt
     2161 cgagttcgtg atggagtaac cggtctcggg gaactccctc gaagcaaccg cggagtggga
     2221 gaacagcgcc aggcagatca ccagggtcgc aaagaatagc gtgatggcgc gggtcatctg
     2281 ttgactcctt tcgatgggtt gattgccgtt agaggccgga gtgccccctg ggacacatca
     2341 cagccgggag agcagaggtg cgtgccgctg attgtaccac gccccaggaa gtggtagttc
     2401 cgctgacatg ggagccaaat ccgtgtacac ttcaccgagc taccactacc tcgatgcgcc
     2461 gtgggttggc ctttacctca gtagccggag acactggaat gatcctggcc gtagtcgcag
     2521 cggtggccat accgctggtc tttctctacc tcgtccgttg gttggatctg tacgcatccg
     2581 gaagtttcag actcgtcgtc ctctgcctgg tatggggctg cgtcgccttt gccctcgcct
     2641 ataacatcaa caccctgttc gtgcccctgg tgggatatgg cctcatagtg accctggtcg
     2701 ctcccatcgt agaggagtgt ctgaagtcgc tggtgctgct atacctcgtc agaaggccac
     2761 agttcaccta tttcgtagac ggcgccatct acggctttgc ctctgggaca gccttcgccg
     2821 tactggaaaa tctcctctat ctgagcaggg cctccgcgga catggggatc atcgtcgtac
     2881 tgagcagggc gttctcaact tcgttgatgc acgggagtgc cagtgcgatg gtcggtgtct
     2941 cacttgggag gttccgcttt gcgcggggcg ccagccggcc tgcttctctc gtccttggct
     3001 ggttggcggc aatggctatc cacatcacct tcaaccatgt catcagcggc gtagagggca
     3061 ctctggctct catcctcgcc gtcgtgctcg gcctcggtgg gatcgccctg acggtggcat
     3121 tcatcatgtg gggattggcc gaggagcgca agtggcttca ggaaaccctg ggcagcgatg
     3181 cgggggtctc gcagggtgag tcggcgctgg tccagaagat ggcggacctc gacctgctga
     3241 tggccccggt ggaggagcgc ttcggcagtg tacgccgaaa gcaggtggag cggttccttc
     3301 agctccaggt acagctcggg ctgaagagca aggcccagac gatgacgcag gacggccggc
     3361 agtccgagtc cctgactggc caaatcgaag agctgaggac ggagatggac gagattcgaa
     3421 ggacggtggg ggtgtactgc atgagctacg tccggtccat ccttccgccc acgactgaac
     3481 ccgtctggga tcgactgacg cagagcttgg agggccagaa caagcccgct ccaaccagaa
     3541 gcctgtggag cgcgctggga gacaagatgg acgccagcga tgggtagccg gggctgccgt
     3601 tccaggcgtt gcgctccagt acaccttcgc attcgcaatc tcgcaaccat cgtcgtcgcc
     3661 ctggcgatcg cctcctttgt tggagtcctg gctcctgctc cgtcgatggt cgcggatgat
     3721 gggcaggtcg agaagagcgt ggcggtggcg cgcacagcgc ctggtcccga gtcggagcag
     3781 gatggggcca tgtgcccctc gccgtccgtt ccccgcatct catccatcaa cggccgtttc
     3841 ctgctcctca acatgcatgg ggcgctcttc cattcggaca tccgcgacct ggaagaggac
     3901 atcacgtacg cccgctggat gaatgccggg gcgataaggg tattcgccac agacaacaac
     3961 acgttcaaga attggagtgg ccggcaggtc ggcaaccgga tagccgatgc ggccccgacg
     4021 ctgagagcca acaacgtcaa actgctcgtc gccctcgtga acaatcacaa gcccgtcccg
     4081 ggagaggccg ctaatagctt cggctggatg gacgacttcg accaacttct ccttcccttc
     4141 tataccgata actggcgcag ggactaccta cccttcgtgc gagagctgat atccacggtg
     4201 atcgcccggg gagcccgaga cgtcatcttc gcctgggagc cgggaaacga gctccacacg
     4261 acccgaaacc ctcaggccat attgccgttc ttcaatcagg tgatcgagga gatcaagaag
     4321 gtcgatcccg agacccccat cctgccgggt accatgggtg tggagcatct gaaccccggc
     4381 aacccgctct cgcccctggg gccgtggatg tattgccacg cgcctgtgga cggctacacc
     4441 ctccacgtat acgactggct cagccctcag cgccagggcg actccacggt ggctaacgac
     4501 ctcaatatcg tcctgtcaca gccttgctgg aacggacgca ggctgccggt gatagtggag
     4561 gaattgggga cgtctcggac gatgccgggg gtctactcca ccgaggagga ggagaagcgg
     4621 ctccaacagg agctcaatca actcaggtac ctgctctcgc atcccgacgt gaaggtaatc
     4681 ggagtggggc catggaacgc ggagagcccg aaggtgaagg aaaagctgtt ctacgacgac
     4741 aggcggggct tcacatcata cgggccatcc agagacggca gcggctcgtg ctacacgggt
     4801 gagagcggcg tccgctgccg cttggagcaa ctgtacagga acctgcccgc actcccgtag
     4861 acggcggcct ccgacagcgc acaccgccgc atccgaggtc gtcacgcccc cggatgaacc
     4921 ttgatctcca catcccactg gggaccctcc aggcgggtca ccgcctctcc tccgagcatc
     4981 cgcaggtcga actcctcggt cccgccggaa gcgttgagaa ctgcaaaggc cgccagcggc
     5041 tccgaatcgc cctcttcgtt gatgcgatga gcccacagct tcaactcgtc tgccccacct
     5101 tcagggagag acaccgtcat gaagagaggt ccagcaccag gaagtacgcg gtccgaggcg
     5161 agagaaggag tctccctcag accacttgcc tcgaccttcg cttccacagc gattcccgac
     5221 aggatcgcgc tcaccgacag cgacggcttg ccagagtcct gcctgagctc gatcaccgcc
     5281 ctggaccgga gtgctccccg acggatgacc gtaaccgtca tacccaggac gatggcggtc
     5341 gtcaccagtg ccgccgcctt ctcgaggggc tgctgccaga tgaccagacc gtggagcaga
     5401 attgccgcca ggaagatgac gtatatgact ccctgcagca cagggttgcc cagcactctg
     5461 tagttgaacc ccggcaccat gtctcccttt cgacggctgg agaggatcag caatacaggt
     5521 atgatcccta cgaagatggt gaccgatatc actcccagca ggttgagaac ggctgtgaag
     5581 gagctggagt ctgtcaccag cgcccactcg gagaagagaa aggcgaggat caccggagtc
     5641 agcgagatga ggaacctgcc acgttccccg gtgaagaggg ccgatagcct ccggcgaatg
     5701 cccgccaggg cagacgtgtg ttgcttctcc ggcagcccct cctccgcctg gggagcgtcc
     5761 tccagcgcgc tccacacctc actggagagt gtcctcccct tccttcgaga tagccgcgcc
     5821 ctatagcttg tggccccacc ctcgggtctt acctcgagca cctgcgactg ggacaccaac
     5881 cgagcgatga gtgtcttgca ctctttctcc tccattccca gttgctcgac aatctccggg
     5941 agggtagcct ctcccttcct gaggagccag gagagcagtt gggtattggc ctcggatgca
     6001 ctgaggacgt ccagcacgct gagcccggcc ccgctccatc cctcttcgca cgtgatagct
     6061 aaaggggttg ccaccgtcaa ccggatgtgg tcgggcccgc tgtccacgag agtcagagag
     6121 tagcgagcag gtgagccatt caacgatggc aagatggccg atagtcgcga ctggtcggcg
     6181 acggcctcca gggtggcggt ggctcgctcc gttctgccgg aacagcgaca ggcgacctcg
     6241 aggcgagggc tgccgtccac gatgccccgg tatgtgaacg cgagggtgaa gctcgcgtta
     6301 ccttcagatc gcgatcccag caccagccgt gagttgtctt tcgtgagcag caacacgggg
     6361 tgcgagtcgt ggcagtctct ccttcaccag attgaagagc cccagagaga agtgaatgga
     6421 tgtcatcccc atagccagaa gcacgaagat cgaccctatc gggtggacca ccggcccgac
     6481 gacggttgcg aggggagaca acgaggtgcc cttctcggag atcaggatct ccgtgggaac
     6541 ggcgccgttc acggccacca cccagacgca gaagaggagc atcgcggcca actccgcgca
     6601 gacaacgccc catatcaacg atctgccgga tggatcccgt cttagcacca gcttgccgca
     6661 attgccgacc gaggtgtggc cgaagtaggt acacaggacg actccaaata ctagctcgag
     6721 gacggacggg tcaaagggac gcccatcgac gaagggcagg ttgatgtagc cgaggaggcc
     6781 agggttcatc cgggagaagg ccaggacaca aagggcgaac accagcagca cgtttacggc
     6841 tcccacgaca agggcggacg caatggtgga atccagcgac cctcgggaga ggaagtagat
     6901 ggcagtgacg aagagcagcg ccgcccacaa ctctggaacg atccccgtgg agctgtagag
     6961 cgtcgaagcc agccccagat agtatgagac caggccaaga aagaatatgc ttgcaagcgc
     7021 caccgtcatc accgacgcgc ccgccgtccc caggtatccg gtgacgatgc ggccgaagta
     7081 gctgcctccg taccggacgc tcccggtgcg cgtcaccgtc tctgccatcg ccgcgatggt
     7141 aatgacgttg atcaatccga tcacgatcag caaagcgacc ccggccgcgg ggcccacctt
     7201 cgccatggcg atcgggagag ccagcacccc ggaacccacc gtttcggtga gcgtcagggc
     7261 aaatacggcc cagaacggag gtagcgtctc cagtgaggta gatacgcgag acgatgcgta
     7321 accgagcctc tccctgacgg agacgggagc ggagaactgc tcttcagcgc tcttgccatg
     7381 cgcccggctg aacgcggccg cgacctccgg agcatcgagc cctgccgccg ctctgatacc
     7441 aggtatggag cggtagttga accggtactt ctgtccgagg gcgtagagaa gcgctgccct
     7501 cagagcgggg ttgtcgggaa ggagaggcgc ccactcaggg gcataccgct ctatgtcctg
     7561 tatggagggg cgaatcggtg gatccttgcc aagggcgaag gactcgagga agtccaggta
     7621 ctgctttcgc tccccctcct cactgtcgta caacgccatc gcccgctttg acctggccgc
     7681 caggtaggct gtgcggttct cgatcaggta gaggagggtg cgggcgcgtc tcgcctgcat
     7741 cccgccaagg atttctccgc gggagaagtc tgctgaagat gacatatggt accgtacctt
     7801 gtaagagaga taagaactgg gtaagagaga taagaaccgg ccctctacct attccagtca
     7861 ccgtctggct ggaaacggtt gtgcccccga ttcgaccctg acctgcaagt cccactcgcg
     7921 cgtatccaat tccaccagac gctctccacc cgtaagccgt aggtcaacct cttcggcccc
     7981 gcgcgatgag ttgagaaggg cagaggcggg tagcggctcc gagttgccgt cttccttcac
     8041 ccgatgtgcc cacaccttca actccgcggc cctccaccgt ggcattgaga tgccgagggt
     8101 cagtaacccg gaggtgggga cggcgtaacc tacagccgta ataggttggc cgcgcatgcc
     8161 ggccagcgcg atgcacgcgt cggaatactc gccagcctgg atcgctgtta ccttgagaga
     8221 ctcttccccc tcttcctgcc tcagttccac caccgccctg gatctcatgg ccccatggcg
     8281 caataccacg acggtcatca cgacgatcag tagcgccgag acgaaggccg caagcttcag
     8341 cagagggtcc tgccagatga ccattccatg ggccaggatt gccccgacga agaagaggta
     8401 gaccgcaacc tgcaacgggg cgctccccag aaggcgactt ctgtagccgg gtaccatgtc
     8461 acccttgcgc cggctggaga ggatgagaaa agac
//
LOCUS       DATALS010000452         8077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_440047, whole genome shotgun sequence.
ACCESSION   DATALS010000452 DATALS010000000
VERSION     DATALS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440047"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VHS06_09985"
     CDS             complement(<1..400)
                     /locus_tag="VHS06_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2988486.1"
                     /translation="MRRGNPNRIALSLLLLAIAAAVIAGWFLTADSSSRSGATPGDTA
                     LMLAVLDVGQADSIFLRSPTGRTILVDGGNARSDAEKVILPFLARQGVSKLDYLVLTH
                     PDQEGERGHGQSVAEQQPPGDRRSHANSTRS"
     gene            complement(441..1349)
                     /locus_tag="VHS06_09990"
     CDS             complement(441..1349)
                     /locus_tag="VHS06_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2988487.1"
                     /translation="MAHLTPARGATQAPKGVEPTSGAVRVAPSVTWRLLLRDRLAVAS
                     LSLLILLALAAILAPFLAAHAPSHIDLKQAALPPGPGHPMGTDLLGRDVWSRALYGGR
                     VSLAVGLSAAAIAALLGVILGMAAAYRGGWVDAVVTRLVDAALAMPAFFLLVAVQSIL
                     GPGVANVILMVSLVGWMVVARVVRALTISLKEREFVLAARALGCSAPRIVLRHLVPNL
                     AGQVGVLFALGVADALLLESALSFLGMGVPLTEPSWGNMLNDAQAAILSGAWWIAAFP
                     GMLVLGTALAINLVGDGIQEMLTPRS"
     gene            complement(1353..2312)
                     /locus_tag="VHS06_09995"
     CDS             complement(1353..2312)
                     /locus_tag="VHS06_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2988488.1"
                     /translation="MSRQIGSRAAQAALLLFLVSLATFSLVRLVPGDPGAFFYGTSAS
                     AEDIALFRSRWGLDAPIHTQYLRWLANAAGGDLGRSYQDGRPVLAVVAERIPASLLLT
                     GTALLVATVIGVGLGIFAAGRRNSRWDRGVTLVATLCYSTPAFWLGIVLILVFSVWLG
                     WLPSGGIRNPAGTSDLGDLLRHLTLPAAALALRDVGRFARITRSSMLEVLSQEYLQTA
                     AAKGLRSRTIALRHTFRNALLPIITLLGMSVPGLLSGSVIIETVFGWPGMGRLAIEAA
                     IQRNYPVIMGEVLIVATLALLGSLLADIAYSVADPRLGRSERG"
     gene            complement(2309..3865)
                     /locus_tag="VHS06_10000"
     CDS             complement(2309..3865)
                     /locus_tag="VHS06_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEX2988489.1"
                     /translation="MARKLYSAIILVLVLALTAGCSPQPAPRPNAIVYGTTFQPNTLN
                     PITAPDIVSRSAIEMIFSGLVSANEKSELRPALAESWESSADGRQWTFKLRHGVKWHD
                     GQDFTAEDVKFTYDTVIDPNTKPTVAKADYAGLQKVEVVDPYTVRFHLSQPNAAFLAR
                     LVLGIAPKHLLQAQDLATTPFNRQPVGTGPFLFDSWAQGESLTLRRNPNYFGEKPKID
                     KIVWKVVPDSNVLALQASNGEVDGAPVFNPKDAAALQSSGKLALHETLEGNTQISLQL
                     KNPIFQDARVRQALAYGIDTRSLIDNILAGAAVPATSDLLPTSWAYNPDVPTYGYDQT
                     KARTLLADAGWKSGTDGILTKDGKRFSISLMTDAGHKVREQVMLAVSQYWSELGIEVK
                     AATQERNSFIFNKVLKGDFDAVLLQSAVQIDPDPSRRFHTNSIRNGQNFLNYSNPRVD
                     ALLEQALATSDQGKRKQAYSEIQRLLAEDLPEISLFYPKTQYAFKTQIQGINPSPSNL
                     FWNAEQWEWK"
     gene            complement(4060..5781)
                     /locus_tag="VHS06_10005"
     CDS             complement(4060..5781)
                     /locus_tag="VHS06_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HEX2988490.1"
                     /translation="MSTVTLTIDGRQVQAEAGSSILQAAQQAGIHIPTLCYHPDLTVR
                     AVCRICVVEVKNARTLQAACSYPVTEGLVVTTNSPRIRASRKVNLELLLSNHPFNCPT
                     CVRNQNCELRALANEYNITQVRFEGERKEYLIDSSSYSLVRDPNKCILCRRCVDVCNS
                     VQAVGAIGPVHRGFDTVIGPAYDAKLSEVACAMCGQCINRCPVGALYEKDAIDDVWEA
                     LADGSKQVAVQVAPAVRVALGEEFGMEPGQIVTGKLAAALRRLGFDAVFDTNFSADLT
                     IMEEGYELIDRIQNGGVLPQMTSCSPGWIKYIEHFYPELLPNLSSCKSPQQMMGALIK
                     SYYAEKRGIDPANIVSVSVMPCTAKKFECERPEMTDSGFKDVDYVLTTRELAKMIREA
                     GIEFNALPEEGFDSPLGAGTGAAVIFGATGGVIEASLRTAYEVLTGTELKKLDFESVR
                     GMEGIKQATVAVGDLPVKVAVAHGLSNAKALMEKVKAGEADYHFIEIMCCPGGCIGGG
                     GQPVPTTPEIRKKRAEAIYREDRGLPLRKSHENPEVQEIYREFLVKPLGEKSHHLLHT
                     HYTPRPR"
     gene            complement(5815..7572)
                     /gene="nuoF"
                     /locus_tag="VHS06_10010"
     CDS             complement(5815..7572)
                     /gene="nuoF"
                     /locus_tag="VHS06_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX2988491.1"
                     /translation="MHAVLHELEALNLDATVVATGCVGLCSQEPLVDIEQPGGLRVTY
                     GKVNPQMVPTLISEHLVKGHVLTQWAVARGSTTPAPVRPQPETVKAIPTYDDIPFYGK
                     QVRIALRNCGVINPEEIQDYIVRGGYEALGRAIFEMTPEEIINTVKQAGLRGRGGAGF
                     PTGLKWELTRKAPGDVKYIICNADEGDPGAFMDRSILEGDPHSVVEGMAIAARAIGAQ
                     EGYVYCRAEYPLAIQRLQKAIEEAHERALLGKNILGSDFSFDLKIKQGAGAFVCGEET
                     ALIASIEGRRGEPRPRPPFPAVSGLWGKPTSINNVETWANVPPILLHGPEWFSSIGTE
                     KSKGTKVFALTGKVNKPGLVEVPMGITMQEIIYDIGGGIPAGKKFKAVQTGGPLGGCL
                     PTSMLDTPVDYDSLTSAGATMGSGGMIVIDEDTCMVEFAKFFLTFATDESCGQCTPCR
                     VGGKRMLEVLTRITDGKGTLNDLKEIDQLAKTMADTSLCALGQLTPNPTLSSLRHFRE
                     EFLAHIVDKKCPAGVCTALLHFEIVADKCKGCSLCLINCPSGAIEGARKQVHTIDQDK
                     CIKCGSCYDVCRLDAVIKS"
     gene            complement(7799..>8077)
                     /locus_tag="VHS06_10015"
     CDS             complement(7799..>8077)
                     /locus_tag="VHS06_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX2988492.1"
                     /translation="YSQFYLNRRGKNIIKVCDGTACHVRGAARTVDGIHTDLGVNPGG
                     TTDDYNFTFEVVYCLGACALAPVAVVNGQVKARCTAERMRQTIKEMTK"
BASE COUNT         1396 a         2487 c         2671 g         1523 t
ORIGIN      
        1 cacttcgggt gctgttcgcg tggctccttc ggtcacctgg cggctgttgc tccgcgaccg
       61 actggccgtg gcctctctct ccctcctgat ccgggtgggt gagcaccagg tagtcgagct
      121 ttgagacgcc ctggcgcgcc aggaagggga ggatgacctt ctccgcgtcg gagcgcgcgt
      181 tgccgccgtc caccaaaatg gtgcggccgg ttggggagcg gaggaagatg ctgtcggcct
      241 gcccaacgtc gagcaccgcc agcataaggg ccgtgtctcc cggcgtggcg ccgcttcgac
      301 tgctggagtc ggcggtcagg aaccagcccg cgatgacggc cgccgcgatg gccagtagca
      361 ggagggacag cgctatccgg ttggggttgc ctcttctcat gccatctagc tcacgcttat
      421 tctatgtcta tgggaagcaa tcaggatcga ggggtcagca tctcctgaat gccgtcgccc
      481 accaggttga tcgccagggc ggtccccagc accagcatgc cggggaaggc tgcaatccac
      541 caggcccccg acagtatagc agcctgggca tcgttgagca tgttccccca gctgggttcc
      601 gtcaggggga cccccatacc cagaaagctg agggcggact ccagcaatag agcgtcggcc
      661 acgcccaggg caaatagcac tccaacctgc cctgccaggt tggggaccag gtgcctaagc
      721 acgatccgcg gcgccgaaca gccgagggct cgagctgcca acacgaactc tcgttccttg
      781 aggctgatgg tgagtgcccg gactacgcgg gccaccacca tccaccccac cagcgacacc
      841 atgaggatga cgttggccac gcccggcccc agaatggact gcacggcgac cagaagaaag
      901 aaggcgggca tggcgagagc cgcgtccacc agccgcgtta cgacggcgtc cacccagccc
      961 ccacgatagg cagcagccat ccccaggatg acgcccagca gcgccgcgat tgccgccgcg
     1021 cttagcccca cggccagcga gaccctgcca ccgtagaggg ctcgactcca gacgtcgcgc
     1081 cccagcaggt ccgtacccat ggggtggccg gggccgggag gtagtgccgc ctgtttgaga
     1141 tcgatgtgag agggggcgtg ggcagccagg aacggagcga ggatcgcagc cagggccaga
     1201 aggatcagga gggagagaga ggccacggcc agtcggtcgc ggagcaacag ccgccaggtg
     1261 accgaaggag ccacgcgaac agcacccgaa gtgggctcga ctccctttgg tgcctgggtc
     1321 gcaccgcgtg cgggcgtgag gtgcgccatg cctcaccccc tctccgaccg accgaggcgg
     1381 gggtcggcca cggagtaggc gatgtccgcc agcagactgc ccaggagggc cagggtggcg
     1441 acgatgagca cctctcccat gatcacgggg tagttgcgtt ggatggcggc ctcgatggca
     1501 aggcggccca tgcccggcca cccgaagacg gtctctatga tgaccgagcc gctgaggagg
     1561 ccgggtacgg acattcccag cagggtaatt atggggagca aggcgttgcg gaatgtgtgg
     1621 cgcagggcga ttgtgcggct tctcagcccc ttggcagccg ccgtttggag gtactcctgg
     1681 ctcagcactt ccagcatgga ggagcgagtg atgcgggcga agcgtccgac gtctcgcagg
     1741 gccagggccg cggcgggaag cgtcagatgg cgcagcaggt ctcccaggtc ggatgtcccc
     1801 gccgggttcc tgatgccgcc agaggggagc cagcccagcc acacggagaa gacgagaatg
     1861 agcactatgc ccagccagaa ggccggggtg gagtagcaga gcgtcgccac tagcgtgacg
     1921 ccgcggtccc atctggaatt gcgacgaccg gcggcgaaga tgccgagtcc gacaccaatc
     1981 acggttgcta ccaggagcgc ggtgccggta agcagcaatg atgccggaat gcgctccgct
     2041 acgacggcga ggacgggacg cccgtcctgg taggagcggc ccaggtcacc gccggcggcg
     2101 ttggcgagcc agcggaggta ctgggtatgg atgggggcat ccagccccca ccgagagcgg
     2161 aagagcgcga tgtcctcggc ggaggccgat gtgccgtaga agaatgcccc ggggtcgccc
     2221 ggaaccagcc gcaccaggga gaaggtggcg aggctgacca ggaagagcag cagggcggcc
     2281 tgggctgccc tgctgccgat ctgcctgctc acttccactc ccactgttcg gcgttccaaa
     2341 agaggttgga gggagaggga ttgatcccct ggatctgagt cttgaatgcg tactgggtct
     2401 tcggatagaa gagagatatc tctggcagat cctccgccag aagtctctgt atctcagagt
     2461 atgcctgttt ccgcttcccc tgatcggacg ttgccagcgc ctgttccaac agagcgtcca
     2521 ccctgggatt gctgtagttc aggaagttct gcccgtttcg gatggagttg gtgtggaagc
     2581 gacgcgaggg gtcgggatcg atctggacgg cgctctgcag caggacggcg tcgaagtcgc
     2641 ccttcagcac cttattgaag atgaaggagt tgcgctcctg ggtggctgcc ttcacctcga
     2701 tccccagctc cgaccagtac tggctaaccg ccagcatgac ctgctcccga accttgtggc
     2761 cggcgtcggt cataagggag atagagaaac gcttgccgtc cttggtgaga attccgtctg
     2821 tgcccgactt ccatcccgcg tcggccagca gcgtccgggc tttggtctgg tcgtatccgt
     2881 aggtggggac gtcgggattg tacgcccagg atgtgggcag caggtctgac gtcgccggaa
     2941 ccgcggcccc cgccaggatg ttgtcgatca aggagcgggt atcgatgccg taggcgagcg
     3001 cctggcgaac ccgagcatcc tgaaagatcg ggttcttcag ttgaaggcta atctgtgtgt
     3061 tgccctccag agtctcgtga agagcgagtt tgcccgacga ctgcagcgcg gccgcgtcct
     3121 tgggattgaa gaccggcgca ccgtcaacct cgccgttgct tgcctggagt gccagcacgt
     3181 tgctgtcggg cacaaccttc cagacgatct tatcgatctt cggcttctct ccaaagtaat
     3241 tggggttgcg cctcagcgtc aggctctccc cctgggccca actgtcgaac aggaagggcc
     3301 cggtgccgac cggttgccgg ttgaaagggg tggtggcgag gtcctgggcc tggaggaggt
     3361 gcttgggcgc tatccccagg acgagccgtg ccaggaaggc cgcgttgggc tgcgagaggt
     3421 ggaagcgaac cgtgtaggga tccacaacct ccaccttctg aaggccggcg tagtcggcct
     3481 tggcaacggt gggcttagta ttgggatcga tgaccgtgtc gtaggtgaac ttcacgtctt
     3541 cggcggtgaa gtcctggccg tcgtgccact tcaccccatg cctgagcttg aaggtccact
     3601 gtcttccgtc ggcagaactc tcccaactct ccgccaaggc cgggcgaagc tccgatttct
     3661 cgttggcaga taccagcccc gagaagatca tctctatggc cgaccgggag actatgtccg
     3721 gcgcggtgat tgggttcagt gtgttgggtt gaaaggtggt gccgtagacg atggcgttgg
     3781 gcctgggcgc gggttggggc gagcagcccg cggtcaaggc gagaacgagt accaagatga
     3841 tggccgagta gagctttctt gccattgtga ggtgtcctct attgtgagtc gtggggttag
     3901 gcctcagccc cgccctacac tcgttgcacg cctgtcctat cggcggccct agtgtagcat
     3961 ggcgggatga gagaggggcg ggttgagaca acccgcccct aatgctttcg tgccccggcg
     4021 ggttgagaca acccgccctc ctagatcagg cagagcccgc tatctgggtc gtggtgtgta
     4081 gtgggtgtgg agcaggtggt gggacttctc gcccagcggt ttcaccagga actccctgta
     4141 gatctcctgg acctccgggt tctcgtggga tttgcgcagc ggcaggcccc tgtcctccct
     4201 gtagatcgcc tccgccctct tcttccgaat ctcaggcgtc gtgggcaccg gctgcccgcc
     4261 cccacctatg cacccacccg gacagcacat gatctcgatg aagtggtagt cggcctcccc
     4321 ggccttcact ttctccatca gcgccttggc gttggagagt ccgtgggcca ccgcaacctt
     4381 taccggaagg tctccaacgg ccacggtcgc ctgcttgatc ccctccatgc cgcgcacact
     4441 ctcgaagtcg agcttcttca actcagtccc ggtaagcacc tcgtaggcgg tcctcaacga
     4501 cgcctctatc acgccaccgg tggccccgaa gataactgcc gctccggttc ccgcccccaa
     4561 tggggagtcg aagccctcct caggcagggc gttgaactca atccccgcct cgcggatcat
     4621 ctttgccagc tctctggtcg tcaagacgta gtctacatcc ttgaagccgc tatcggtcat
     4681 ctcaggccgc tcgcactcga acttcttggc cgtgcagggc atcaccgaca cagacacgat
     4741 gttggcgggg tctatccccc tcttctcggc gtagtagctc ttgatcaatg cgcccatcat
     4801 ctgctgtggg gacttgcagc tggacaggtt gggcagcagc tccggataga agtgctcgat
     4861 gtacttgatc cagccggggg agcagctggt catctggggc aggacgccgc cgttctggat
     4921 cctgtctatc agctcgtagc cctcctccat tatggtcagg tccgcgctga agttggtgtc
     4981 gaagacggcg tcgaacccca gccgtcgtag cgccgccgcc agtttgccgg ttacgatctg
     5041 ccccggctcc atgccgaact cctcgcccag ggcaacccgg acggcgggag ccacctgcac
     5101 cgccacctgc ttggagccgt ctgccagcgc ctcccagacg tcatcgatgg cgtccttctc
     5161 gtagagggcg cccaccgggc atcggttgat gcactggccg cacatggcac acgcgacctc
     5221 ggagagcttg gcgtcgtagg cgggaccgat gacggtgtcg aatccccgat ggacgggacc
     5281 gatggccccc acggcctgga cggagttgca gacgtccacg cagcgcctgc acaggatgca
     5341 cttgttgggg tcgcggacca gggagtagga gctggagtcg atgaggtact ccttgcgctc
     5401 accctcgaag cggacctggg taatgttgta ctcgttggcg agagcccgca gctcgcagtt
     5461 ctggttgcgg acgcaggtag ggcagttgaa ggggtggttg gagagcagca gctccaggtt
     5521 gaccttgcgg gacgcacgga ttcgggggga gttggtggtt accaccaagc cctcggtgac
     5581 cgggtacgag caagcggcct gcagggtcct ggcgttcttg acctcgacca cgcagatgcg
     5641 gcagacggcg cgaacggtca ggtccggatg gtagcagagg gtggggatgt gaatgccggc
     5701 ctgctgggcg gcctgaagga tgctgctgcc ggcctctgcc tggacctggc ggccgtcaat
     5761 agtcagtgtc accgtcgaca tttgtcgtga cctcctccgg taagctgttc gtctctatga
     5821 cttgatgacg gcgtcgagtc ggcagacgtc gtagcaggat ccgcacttga tgcacttgtc
     5881 ctggtctatg gtgtgtacct gcttcctggc cccttcgatg gcaccgctgg ggcagttgat
     5941 caggcacaga gagcacccct tgcacttatc ggccacgatc tcgaagtgaa gcagtgcggt
     6001 gcagacgccg gcgggacact tcttgtcgac tatgtgagcc aggaactctt ctcggaagtg
     6061 tcgaagggag ctgagcgtcg ggttaggagt cagttggccc agggcgcaca gcgaagtgtc
     6121 cgccatggtc ttggccagct ggtcgatctc cttgaggtcg ttcagggtgc ccttaccgtc
     6181 ggtgattcgg gtcagcacct ccagcattcg cttgcccccc acgcggcagg gggtgcactg
     6241 gccgcaggac tcatcggtgg cgaaggtgag gaagaacttg gcgaactcca ccatgcaggt
     6301 gtcctcgtcg atgacgatca tgccacccga gcccatggtt gcgcccgcac tggtgaggga
     6361 gtcgtagtcc accggcgtgt ccagcataga ggtcgggagg cagccaccca ggggaccgcc
     6421 cgtctggacc gccttgaact tcttccccgc cgggatgccg ccgccgatgt cgtagataat
     6481 ctcctgcatg gtgatgccca tggggacctc gaccaggccg ggcttgttga ccttgccggt
     6541 cagcgcgaag accttggtcc ctttgctctt ctcggttccg atgctggaga accactccgg
     6601 accgtggagg aggatgggcg ggacgttggc ccaggtctcg acgttgttga tgctggttgg
     6661 cttaccccag agaccagaca cagccgggaa ggggggcctt ggtcgcggct cacctctccg
     6721 accctcgatg gatgcgatca gtgcggtttc ctcgccgcat acgaaggccc cggcgccctg
     6781 cttgattttg aggtcgaagg agaagtcgct gcccaggatg ttcttgccga gaagcgcgcg
     6841 ctcgtgggcc tcctcgatcg ccttctgcaa ccgctggatt gccaggggat actcggcgcg
     6901 gcagtatacg tagccctcct gggcgcctat ggctcgcgcc gcgatagcca tcccctccac
     6961 gacggagtgg gggtcgccct ccaggatact gcggtccatg aaggcgccgg ggtcgccctc
     7021 gtcggcgttg cagatgatgt acttgacgtc gccaggggcc ttgcgcgtta gctcccactt
     7081 gagaccggtc ggaaagccag caccacccct tccgcggaga ccagcctgct tgaccgtgtt
     7141 tatgatctcc tcgggtgtca tctcgaagat ggctctgccg agggcctcgt agccgccccg
     7201 aacgatgtag tcctggatct cctccgggtt gatgacaccg cagttgcgca gggcgatccg
     7261 gacctgcttg ccgtagaagg ggatatcgtc gtaggtcggg atggccttca cggtctcggg
     7321 ctgaggtcgt acgggcgccg gggtggtgga gcctcgagcg accgcccact gggtcaggac
     7381 gtgtcccttg accaggtgtt ccgatatgag ggtgggcacc atctgcgggt tgaccttgcc
     7441 gtaggtgacc cgcaggccac ccggctgctc gatgtccacc agcggttcct gggagcagag
     7501 ccccacgcag cctgtggcaa ctaccgtcgc gtccaggtta agcgcttcaa gctcgtgaag
     7561 tacggcgtgc atgacttcgc gagcacccgc ggcgatgccg caggtaccca tgccaatggt
     7621 gatcttcgtg gcggaatcgc cacgcgcact gatctcgctg atggcctgct ctttcagttg
     7681 cctcaactgc tccattgcac tcggttgcgg catcgaactc ctcctcaacg ggcgttccgc
     7741 gagcgcccgg atctgctatg gatggaaagg gcaacacgcc agccgtggag tatgcccgtc
     7801 acttggtcat ttcctttatg gtctggcgca tccgctcggc ggtgcagcga gccttgacct
     7861 gcccgttgac gacggccacg ggggcgagag cacaggcccc caggcagtag acgacctcga
     7921 aggtgaagtt gtaatcatcg gtggtcccac ccgggttgac accgaggtcc gtgtggatgc
     7981 catcgacggt gcgagcggcg ccgcgaacgt ggcaggcagt gccatcgcac accttgatga
     8041 tgttcttgcc acgccggttg agatagaact gggagta
//
LOCUS       DATALS010000453         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_118865, whole genome shotgun sequence.
ACCESSION   DATALS010000453 DATALS010000000
VERSION     DATALS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118865"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1335)
                     /locus_tag="VHS06_10020"
     CDS             complement(139..1335)
                     /locus_tag="VHS06_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988493.1"
                     /translation="MERRIAEHRLTEDALRESEAGLRKLTRAVEYSPATVVITDLQGD
                     IEYVNPKFEALTGYSAREALGQNPRICASGQTPPEVYRDLWVTIKSGREWRGELLNRK
                     KNGDLYWESVSISPTTDEEGNITHFIAVKEDITERKAAEAERERLMTEIENRAAELSQ
                     TVGELRRLKEQREDYIRAISHDLRNPLTSVLGQAQLLLRLLGQEGKAEMVRRSAGAIL
                     TSGQHMNSMIQELADEARLESGQLQLSPKPVSLPDFVLGLRERVGEPEDARRIAVEVP
                     EELPLVLADVDRLERILRNLLSNALKYSPSEAEVTVTMDQADGKVVTSVIDRGVGMCA
                     EEVSNLFQRYYRTESARRAKGGLGLGLYVTKGLVEAHGGEIWVESETGKGSSFHFSLP
                     IAPGQE"
     gene            complement(1372..2166)
                     /locus_tag="VHS06_10025"
     CDS             complement(1372..2166)
                     /locus_tag="VHS06_10025"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HEX2988494.1"
                     /translation="MSTTECGKGTAPIVSAQSIGLALLIAVASIAMAQVDYLLFHTAA
                     EGFAIVVAVLIYVVGTRTYRYSGDGFLQFLGTAYLFVALLDSLHTMAYQGMGVFHLDG
                     PNEATQLWVAGRLVGAASLYLATYFIGRSPSQRLTVCGYALVTAVLVASIMVFDVFPA
                     AYLPGEGLTSFKIGAEYAVCAMVLGAVWRLHSRKDRLDRSVYLLMLAAMGVTVLSELS
                     FTLYTDVYGVMNLAGHLLKILAYYLIYLAIVRRGLERPYREIGRLN"
     gene            2336..2950
                     /locus_tag="VHS06_10030"
     CDS             2336..2950
                     /locus_tag="VHS06_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX2988495.1"
                     /translation="MPINRQLDCDVSPCVDVVKTAPHTYRMEVDPAAVKVVMISEAPP
                     ADPRDDFDAGPAAFFFRTTAQAFRDAGITVTSMQDIADLGVYLTTAIKCPKVGYNVSA
                     RTVDACSALLQKEIDLLPNVKAYLLMGDVALAAMNQIARRMTGIRVVPAGSIYKLRRG
                     VYEYRGARVFPSYLQTGKSYLIEKGKAEFIAQDIRAALDLLSCQ"
BASE COUNT          607 a          973 c          902 g          569 t
ORIGIN      
        1 ggggcgggac acctttctcg aaaacacaga ccgggccgaa tcctgggaag gctatcgctc
       61 caatcggcag ggtgtgcagg gcgacattgc ggcgtcaagc cgccgcactc caaaacgtga
      121 gccgcgctgc agaagggatc actcctgccc gggggcaatg gggagggaga agtggaagct
      181 gctccccttc ccggtttcac tctcgaccca gatctcgccg ccgtgggcct ctaccagtcc
      241 cttcgtgacg tagaggccga gccccagccc tcctttagcc cgcctggcag actccgtccg
      301 atagtagcgc tggaagaggt tggatacctc ctcggcgcac atccccactc cccggtcgat
      361 aaccgaggtg accaccttcc cgtccgcctg gtccatggtg accgtcactt cggcttccga
      421 cggcgaatac ttcagggcgt tgctgagcag gttcctcagg atccgctcca gcctatccac
      481 gtcggccaga accagcggca gttcctcagg cacctcgacg gctatgcgcc gagcatcctc
      541 cggctccccc actcgctcgc gcaaaccaag gacgaagtcg gggaggctca ccggcttcgg
      601 actcagctga agctgccccg actccagcct cgcctcatcg gccaactcct ggatcatgct
      661 gttcatgtgc tgcccgctgg tgagaatggc ccccgccgag cggcgcacca tttcggcctt
      721 gccttcctga cccagcagcc tcagcagcag ctgcgcctgg cccagtaccg aggtcagggg
      781 attgcgcagg tcgtgggaga tcgctcgtat gtagtcctct cgctgctcct tcagcctacg
      841 cagctcgcca accgtctgac tcagctcggc agcccgattc tcgatctcgg tcatcaaccg
      901 ctcgcgttcg gcctccgccg cctttcgctc ggtgatatcc tccttcaccg cgatgaaatg
      961 agtgatgttt ccctcctcat cggtggtggg agagatagac accgactccc aatagaggtc
     1021 accgttcttc ttgcgattca ggagctcgcc gcgccactcg cggccggact tgatggtaac
     1081 ccacagatcc cgatacacct cgggcggcgt ctggccggag gcacagatgc gagggttctg
     1141 tcccagcgcc tcgcgggcgg agtagccggt gagcgcctcg aacttcggat tcacgtactc
     1201 gatgtcgccc tgtaggtcgg tgatcacgac ggtggcgggg ctgtactcca ccgctcgcgt
     1261 cagtttccgc agtccagcct cgctctcccg aagggcatcc tcggtgagcc tgtgctcagc
     1321 tattctgcgc tccagttccg cggttctgtc ggcaacggcc tgctctagcc cctaattgag
     1381 ccgcccaatc tcccgatagg gcctctcgag gccacggcga acgattgcca ggtagatgag
     1441 gtagtaggcc aggatcttca gcaggtgtcc cgccaggttc atgacaccgt agacgtcggt
     1501 gtacagcgtg aagctcagct cggagaggac cgtgacgccc atggccgcca acatcagcag
     1561 gtagaccgaa cgatccaaac ggtccttccg agagtgaagt cgccagacag cccccagcac
     1621 catcgcgcag acggcgtact cggcgccgat cttgaacgag gtaagcccct ccccaggcag
     1681 gtaggcggcg gggaagacgt cgaagaccat gatggaggct accagcaccg ccgtaaccag
     1741 ggcataccca cagactgtca ggcgctggga gggacttcgt cctatgaagt atgtagccag
     1801 atacagggaa gcagcaccga ccaaccgccc cgcgacccac aactgcgttg cctcgttggg
     1861 gccgtcgaga tggaagaccc ccattccctg gtaggccatg gtgtggagag agtccagcag
     1921 ggcaacgaac aggtacgcag tacccaggaa ctgcaggaag ccgtcacccg agtacctgta
     1981 ggtcctggtg cccaccacgt agatgagaac ggcgacgacg atggcaaagc cctctgccgc
     2041 cgtgtggaac agcagatagt cgacctgcgc catcgcaatc gaggcaactg ctatcaacag
     2101 cgccaatcca atgctttggg cgctgacgat tggagctgtg ccctttccgc attctgtcgt
     2161 agacatatcg ccgccacgaa attggtggaa gattgaagag ttcgtgatat attacctgga
     2221 aatagctaac ttattatacc aactcgtcaa ctgcccagga attgtaggga cggcgtggag
     2281 gggtggattg aagagaaccg ccctttcacg aacccaaaga tgtaggagaa gaccagtgcc
     2341 gatcaacagg caattggatt gcgatgtctc gccctgcgtg gatgtggtca agaccgcccc
     2401 tcatacttac cggatggaag tcgacccggc cgccgtcaag gtggtgatga tctcggaggc
     2461 cccgcctgcc gaccctcggg acgactttga cgcgggtccc gccgccttct tcttccggac
     2521 taccgctcag gctttccgcg acgcggggat aaccgtcacg tcgatgcagg acatcgctga
     2581 tctcggcgtg taccttacca cggcgatcaa gtgtccgaaa gtgggctaca acgtgtcggc
     2641 cagaacggtc gatgcatgct cggccctgct tcagaaggag atcgacctcc tccccaacgt
     2701 gaaggcatac ctgctgatgg gcgatgtggc gctcgcggcg atgaaccaga tcgcacgcag
     2761 gatgacgggg atcagggtag tcccggcagg ttccatctac aagctgaggc ggggagtcta
     2821 tgagtatcgg ggggccaggg tgttcccctc ctatctgcaa accggcaaga gctacctgat
     2881 agagaagggg aaagcggagt tcatcgctca ggacatcagg gccgccctcg acctactgtc
     2941 gtgccagtga tgatcgactg atggtagggg cggacctacc acgtgttgac gggtccgccc
     3001 caaccgcaga gcctcagagc ttggtcttga gatccgcgtc caactgctct a
//
LOCUS       DATALS010000454         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_301186, whole genome shotgun sequence.
ACCESSION   DATALS010000454 DATALS010000000
VERSION     DATALS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301186"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /gene="kdpB"
                     /locus_tag="VHS06_10035"
     CDS             <1..1740
                     /gene="kdpB"
                     /locus_tag="VHS06_10035"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020120249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX2988496.1"
                     /translation="KKDTPARRLFDSREEAVSSSDLRRGDMVLIKEGEIVPCDGEVVQ
                     GVAYVNEAAITGESAPVLKEPGTDVRSTVTGGTQVVSDWLKVRITADPGHTFLDRMIS
                     LVEGAVRLKTPNEIALSILLSGLTIVFLLAVVTLEPFAIYAGGPVSTVVLIALLVCLI
                     PTTIGGLLSAIGIAGMDRVTRFNVLATSGRAVEAAGDVDVLLLDKTGTITYGNRLAAE
                     VVPVGGATQEEALNAALRASLGDETPEGKSIVALIGQLGFVPPEGRDGGVPAGAEVIP
                     FTSQTRISGLSLDGRVWLKGAVDAMAARQGMPIPADVQAETDRIAREGGTPLAVSDGS
                     RLVGVIYLKDTVKPGMRERFEELRRMGIRTVMVTGDNPLTAATIAGEAGVDDFVAEAK
                     PEDKIGIIQEQQRLGHLVAMSGDGTNDAPALAQSDVGLAMNSGTAAAKEAANMVDLDS
                     NPTKLIEVVAIGKQLLITRGAITTFSVANDVAKYFAIIPAAFAATYPQLNALNVMRLA
                     TPQSAILSAVIFNALVIIALVPLALRGVGYRPAPAAELLRRNLMIYGVGGIVAPFLGI
                     KIIDLLIAIAGVG"
     gene            1751..2338
                     /gene="kdpC"
                     /locus_tag="VHS06_10040"
     CDS             1751..2338
                     /gene="kdpC"
                     /locus_tag="VHS06_10040"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEX2988497.1"
                     /translation="MELVRQTRSAVLMVVVLTVLVGIIYPLVMTGLAPLIFPYQANGS
                     LMRDAKGNVIGSESIGQQFSDARYFHPRPSVAGDEGYDATASGGTNLGPTNKKLIDSV
                     QERAVAYRQENGLAPDAQVPVDAVTASASGLDPEISPANAQLQVKRVAAARSLPEDQV
                     RALVDKYTRGRTLGILGDPGNVLLAGNACQFLCHI"
     gene            complement(2675..3277)
                     /locus_tag="VHS06_10045"
                     /pseudo
     CDS             complement(2675..3277)
                     /locus_tag="VHS06_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
BASE COUNT          571 a         1061 c         1126 g          625 t
ORIGIN      
        1 aagaaggata caccggctcg ccggctgttc gattcgcgag aggaggcggt cagctccagc
       61 gacctgagaa ggggcgatat ggtgctgatc aaggagggag agatcgttcc ctgcgatggc
      121 gaggtagtcc agggggtggc ctacgtcaac gaggcggcca tcacgggcga gtctgcgccg
      181 gtgctcaagg agccggggac cgacgtccga tccacggtga ccggaggcac ccaggtggtg
      241 agcgactggc tgaaggttcg catcacggcg gatccggggc acaccttcct ggacaggatg
      301 atctccctgg tggagggggc ggttcggctg aagacgccca acgagatcgc cctgtccatc
      361 ctgctttcgg ggctgaccat cgtcttcctg ctggcggtgg tgacgctgga gcccttcgcc
      421 atctacgcgg gcgggccggt ctccaccgtt gtcctgatcg ccctgctggt gtgtctaatc
      481 cccacgacca tcggcggcct gctgtcggcc atcgggatcg cgggaatgga cagggtgacc
      541 cgcttcaacg tgctggccac ctccggtcgg gccgtcgagg ccgctgggga cgtggatgtg
      601 ctgctcttgg acaagaccgg gaccatcacc tacggcaacc ggctggccgc ggaggtggtg
      661 ccggtgggcg gcgcaaccca ggaagaggca ctgaacgcgg cgctgcgggc ttccctgggg
      721 gacgagaccc ccgaggggaa gagcatagtg gcgctgatcg ggcagctcgg ctttgttcct
      781 ccggagggac gtgatggcgg cgttccggcc ggcgcagagg tgatcccctt cacttcgcag
      841 acgaggataa gcgggctatc cctggacggg cgggtgtggc tcaagggggc cgtggacgcc
      901 atggccgcca gacagggcat gccgataccg gccgacgtgc aggcagagac cgaccgaatc
      961 gcccgggagg gaggcactcc gctggcagtg tccgatggct ccaggctcgt cggagtcatc
     1021 tacctgaagg acaccgtgaa gccgggcatg agggagcggt tcgaggagtt gcgccggatg
     1081 ggcattcgga cggtgatggt cacgggagac aaccccctca ccgcagctac gatagccggt
     1141 gaggccggtg tggacgactt cgtcgccgag gcgaagccgg aggacaagat cggcataatc
     1201 caggagcagc agcggctcgg ccacctggtg gccatgagcg gcgatggaac caacgacgcc
     1261 ccggcgctgg cgcagtccga cgtggggctg gccatgaact ccggcactgc cgccgccaaa
     1321 gaggccgcca atatggtgga cttggactcc aatcccacaa agctgatcga ggtggtagcc
     1381 atcggcaagc agttgctgat caccagaggg gccatcacca ccttctcggt ggccaacgac
     1441 gtggcgaagt acttcgccat cattccggcg gcttttgccg ccacctaccc gcagttgaac
     1501 gcgttgaacg tcatgcggct cgccactccc cagagcgcga tactctcggc ggtgatcttc
     1561 aacgccctgg tcatcatcgc gctggtgccg ctggcgttgc ggggtgtcgg ttatcgaccg
     1621 gctccggcgg ctgagttgct gcgacggaat ctgatgatat atggggtcgg cgggattgtt
     1681 gcgcccttcc tggggatcaa gataatcgat ctcctgatag ccatcgccgg ggtgggctga
     1741 ggaggtcacg atggaactgg ttcgtcagac gcgtagtgcc gtgttgatgg ttgtggtatt
     1801 gacggtgttg gtcggaatca tctacccgtt ggtgatgacc ggtctggcgc cgctgatctt
     1861 cccgtaccag gcgaacggca gcctgatgcg ggacgccaag ggaaacgtga tcgggtcgga
     1921 gtcgatcggc cagcagttct cggatgccag gtactttcat cctcgaccat ccgtcgcagg
     1981 cgacgagggt tacgacgcca cagcctccgg cgggaccaac ctggggccga ccaacaagaa
     2041 gctgatagat tctgttcagg agagggctgt ggcctacagg caggagaacg gcctggcgcc
     2101 ggacgcccag gtgccggtgg atgccgtaac cgcctcagcc agcggcctcg atccagagat
     2161 tagcccggcc aatgcgcagc ttcaggtgaa gagggttgcc gcggcccgta gtctgccgga
     2221 ggatcaggta cgggctctgg tagataagta caccaggggg cggactctgg gcattctcgg
     2281 ggatcctggc aacgttttgc tggcgggcaa cgcttgccaa ttcctctgcc acatctgagc
     2341 ccttcaccct cagcaccttc gcacccagat cctcggcaaa gcgaaggttt tcctcgagtt
     2401 gccgcttctc ttcaggggac gcgtgggccc aggacggggt ctcgacgaag accgccacga
     2461 gttcggactg gaaacggtcg gccatgcgcc agcccctgcg caggagatgc tgcgcgtcgg
     2521 ggtgaggccc gacgcacacc atcacccgct cccccgcggg ccacaccgcc tcgatgttgt
     2581 gctggcgcat gtattcgtcc aggtcctctt ccacggctgt ggagagtttc ctcagcgcca
     2641 actctcgcag ggcggtcagg ttgccttctc ggaagaactg gtggagcgcc tgctgggccc
     2701 gctcgggcgg atacacgttg ccgtgcctga tccgctgccg cagcgaatgg ggggccatgt
     2761 ccaccagctc gacctcgtcg gccttgtcca ccacctcgtc agggatccgt tcccggaccg
     2821 gagcaccggt gatccgctcc acgatgtccg ccaggctttc cagatgctgg atgttcaagg
     2881 tgccgatgac ggttatgccg tggtccagca gttccagcac atcttcccaa cgcttcttgt
     2941 gcttggagcc cggcgcgttg gtgtgtgcaa gctcgtccac cagcgcgacc tgggggttgc
     3001 gccggatcac cgcgtccgtg tccatctcct ccagggtggc gcccttgtac tccacctttc
     3061 ggcgaggaac gatctccaga tcgccgatcg cttcgatagt ctgagcccgt ccgtaggttt
     3121 cgacgaaacc tatcgccaca tcggtgcccc gctgcttccg gcgattcccc tccataagca
     3181 tggcgaaagt cttgccgacc cctggagcca tgccaagata gatgcgcagc cggccgcgag
     3241 cccccgctcc ggcctccttg cgcacccgct ccagcatctc ctcagctgtc ggccgaccct
     3301 cttcgcgccg ctgctccact attcccccca accggttctg ggttctgggt tctgagttct
     3361 gagtgctgct ctcacccccg tat
//
LOCUS       DATALS010000455         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_118997, whole genome shotgun sequence.
ACCESSION   DATALS010000455 DATALS010000000
VERSION     DATALS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118997"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VHS06_10050"
     CDS             <1..857
                     /locus_tag="VHS06_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2988498.1"
                     /translation="EQECAQKLRKLIGFLPANNNEKPPYVTPTDSPDRTEERLNTIVP
                     DDPSMPYDMHEVINLILDNNDFLEVHEDFATNLIVGFGRLNGHAVGVVAQQPASMAGV
                     LDINASVKGARFVRFCDCFNIPLITFVDVPGFMPGTMQEHGGIIRHGAKLLYAYIEAT
                     VPKVSVITRKAYGGAYTVMSSKEARGDINLAWPSAEIAVMGPEGAVNIVFKDKIQKSE
                     NPKAAWAEALREYREKFANPYVAAARGYLDDVIEPRETRPQLIKALESLQNKKDSNPP
                     RKHGNIPL"
     gene            939..1343
                     /gene="mce"
                     /locus_tag="VHS06_10055"
     CDS             939..1343
                     /gene="mce"
                     /locus_tag="VHS06_10055"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007959485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX2988499.1"
                     /translation="MIKVLKIEHIGVAVKSRSESAAFFKLLGLKVEGEETVGTDLKVA
                     MLGVGESEIELLEPANPDATVAKFIEKRGEGIHHLALTVEDLDGAVKELLAAGVRMID
                     KAPRPGAGGSMVAFVHPAAAKGVLIELVQSRK"
     gene            complement(1461..>2161)
                     /locus_tag="VHS06_10060"
     CDS             complement(1461..>2161)
                     /locus_tag="VHS06_10060"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX2988500.1"
                     /translation="GAQATTAAPAWWQGNRLTGHSSSGSAASASSASDPLPTVVGKHV
                     GIQAGHWQTANLPDELASLRGSTGAAGNGWREVDVNLDIARRVSALLGKSGVQVDLLP
                     STVPIGYQADAFVALHNDGSSNTSISGFKVARSSWSVIPNKDDALVMDLEADYGAGTG
                     FEPNSQTITLAMTRYYAFNNRRLEHSVSPSTPAAILEMSFLTNPGERSVMMNDPDRVA
                     KAIARGILDFLSEK"
BASE COUNT          461 a          640 c          685 g          375 t
ORIGIN      
        1 acgaacagga gtgcgcgcag aagttgcgaa agctgatagg cttcctcccc gccaataaca
       61 acgagaagcc cccctacgtg actcccaccg actcgccgga ccgcacggag gagcgactca
      121 acactatcgt gcccgatgac ccctccatgc cctacgacat gcacgaggtg atcaacctca
      181 tcctggacaa caacgacttc ctggaggtcc acgaggactt cgcaaccaac ctgatcgtgg
      241 gcttcgggcg gctgaacggg catgcagtag gagtcgtcgc gcaacagccc gcctcgatgg
      301 ccggtgtact ggacatcaac gcgtcggtga agggcgcccg cttcgtccgc ttctgcgact
      361 gcttcaacat cccgctgata accttcgtcg acgtgcccgg cttcatgccg gggacgatgc
      421 aggagcacgg tgggatcatc cggcacggcg caaaactgct ctacgcctac atcgaggcca
      481 cggttcccaa ggtctccgtg atcacgagga aggcctacgg tggggcgtac acggtgatga
      541 gcagcaaaga agcccgcggc gacatcaacc tggcgtggcc aagcgcggag atcgcggtga
      601 tgggtcccga gggtgcggta aacatcgtct tcaaggacaa gatccagaaa tcagagaacc
      661 cgaaggccgc ctgggccgag gcactaaggg agtatcgaga gaagttcgcg aatccctacg
      721 ttgccgccgc caggggatat ctggacgacg tgatcgagcc aagagagacc aggccgcagt
      781 tgatcaaggc gttggagagt ctccagaaca agaaggactc caatccgccg aggaagcacg
      841 gcaacatccc gctgtagggg atgggagatg ggggttgggg gttagggatg tcccaaccct
      901 cggccaacga tactgcacag caagtgtgga gttgagaaat gataaaggtt ctgaagatcg
      961 agcacatcgg ggtggcagtc aagagtcgct cggagtcggc cgccttcttc aagctgcttg
     1021 gcctcaaggt cgaaggcgaa gagaccgtcg gaaccgacct caaggtggcg atgctgggtg
     1081 tcggggagag cgagatcgag ctgctggagc ccgcaaaccc cgacgcgacc gtcgcgaagt
     1141 tcatcgagaa gcgaggagaa gggatccatc acctggcact caccgtcgag gacctggacg
     1201 gcgccgtcaa ggagttgctg gccgctgggg tcaggatgat cgacaaggcg ccgaggccgg
     1261 gtgccggtgg aagcatggtc gccttcgttc accctgcggc cgctaagggc gttctcatag
     1321 agctggtgca gagccgcaag taagtcaaac actcgcattt ctcaaagcaa ggccaaacag
     1381 acaatagcag gtcgacagag cgaacgcccc ggggatgtcc ccagggcgtt cgtttgctgt
     1441 ctcgcattat ggtgtcagat ctacttctcg ctgaggaagt ccaggatccc tcgggcgatt
     1501 gccttggcca cccgatccgg gtcgttcatc ataacgcttc gctccccggg attggtcaga
     1561 aagctcatct ccaggattgc ggccggcgtc gacggcgaca cggagtgctc cagcctccgg
     1621 ttgttgaagg catagtagcg ggtcatagcg agtgtaattg tctgcgaatt tggctcgaag
     1681 ccggtgccgg ccccgtagtc ggcttcgaga tccatcacca gggcgtcgtc cttgttggga
     1741 ataacgctcc aactggaacg ggcgaccttg aaaccggaga tgctcgtgtt ggagctgccg
     1801 tcgttgtgca gtgccacgaa ggcatcggcc tggtagccga tggggaccgt ggacggcaga
     1861 agatccacct gaacaccgga cttcccgaga agcgccgaca ctctccgcgc gatatccagg
     1921 ttcacgtcca cctcacgcca accgtttccg gccgcccctg tggagcctcg cagacttgcc
     1981 agttcgtcag gcaggttggc ggtctgccag tgcccggcct ggatgcccac atgctttccc
     2041 acaaccgtcg gcagcggatc agacgccgag gaagcagacg cggcgctgcc ggatgacgag
     2101 tgtcctgtca ggcggttgcc ctgccaccaa gctggagccg cagttgttgc ctgtgctccg
     2161 c
//
LOCUS       DATALS010000456        10816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_119049, whole genome shotgun sequence.
ACCESSION   DATALS010000456 DATALS010000000
VERSION     DATALS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 10816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10816
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119049"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VHS06_10065"
     CDS             <1..1405
                     /locus_tag="VHS06_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX2988501.1"
                     /translation="IGPVTGVAILIPITFGMNPTTAMIMLAGIYYGAMYGGSTTSILV
                     NVPGESASVMTAVDGYQMARQGRAGAALGMSAIGSFVAGTFSVVGLMVVAGPIVSLAL
                     SFGPPEYFALMLLGLSTLTRLAGKSLLKGLAMGFAGMLIGTVGLDPIAGVARFTFGRP
                     DLMDGIGFVSVAMGLFAFGEILTNLEGSLDREIFSSKIGSVLPTMQDWIDCKWTLLRS
                     TLIGFVIGSLPGAGATVAAFMAYAVEKKASKHPERFGNGAIEGVCASESANNAATGGA
                     LVPLLTLGIPSSATVAIMMGALMMYGLRPGPLLMAENPEFFWGLVASMYVGNAMLLVL
                     NLPLVGMWVKILKVPYPVLIPLILLFVLLGAYTVGGAISDLWIMFAFGVVGYLMRKFD
                     YPAAPMVLALVLGPIMEKNLKLSLTMSQGDLTIFATRPIPLILFALTALSLGWPALVS
                     LLSRGKTVVEMPAAEEV"
     gene            1459..2496
                     /locus_tag="VHS06_10070"
     CDS             1459..2496
                     /locus_tag="VHS06_10070"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX2988502.1"
                     /translation="MVVSVGTGGERVSRLPRLAIAMGDPAGVGPEIIVKALAEPEIYD
                     FCEPVVIGDAGVLMRAPGWPNGEPRLDLVDDVESACPRAGRVSLLNLGNVPPSLNVGE
                     ASAEGGRACLEYLEKITRLAIEGKVDAVVFGPLNKEAMKKAGSPHTDEYGLFARMCGV
                     DDYSVLMVGPHFTLASVTLHLPFSEVSRHITKERVLSTIRHGERAAQAAGVEHPRIGV
                     AALNPHAGEGGTLGKEEQDVIGPAVAAAVAEGHDVEGPFPADTFFMTTREPRYDVYVG
                     MYHDQGRIALKLLDFGRVTTMAEGLPAFFCTVGHGTAYDIAGKGIAHHENMRDTLLLA
                     ARRAAAHRRGK"
     gene            2591..3367
                     /locus_tag="VHS06_10075"
     CDS             2591..3367
                     /locus_tag="VHS06_10075"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2988503.1"
                     /translation="MRLEGKVALVTGAGSGIGRAIAVRLAGDGARIVAADLNPEGATE
                     TISKIEAAGGSGSAVQVDVRDVSSLQAMVDGTVERFGRIDILVACAGIVQTKRMRDIT
                     PEDWDRIFSVNSRGLFFTMQLVSKQMERQGSGVILNMSSAAARGPRPMYTHYAASKAA
                     VISITRSAAASLAPSGVRVNAICPGIVETPMWDQIDREYAAESGRPLGEHRRQRAKEI
                     PLGRLETAEDVASAAAFLVSDDASYITGQTLNVDGGINMD"
     gene            complement(3385..4317)
                     /locus_tag="VHS06_10080"
     CDS             complement(3385..4317)
                     /locus_tag="VHS06_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX2988504.1"
                     /translation="MKVEVEVVDSTQRNVNDTAAGETLLACFAHPDDEAFGSGGTLAR
                     YASEGARVVLVCATAGEVGEISDPALASPENLGEVRREELRCSARQLGASDVIFLGYR
                     DSGMAGTADNENPKAFAKARDEDVVSQLVGIMRDLKPKVVVTFEPGGGYGHPDHIAIS
                     RHTAAAFVAAGDPKRFPERGAAWQPSRLFYTAIPRSFFIEMRDRLQEMGIDTSDFARF
                     EEAGAGWPDEKINVTMDISEFIEAKWTALHCHRTQLSPDNLFFKVPEQVVKQGFSRES
                     FALAIPEPRQGERLSGLFAGLKRNGTGTTPLSKG"
     gene            4660..5847
                     /locus_tag="VHS06_10085"
     CDS             4660..5847
                     /locus_tag="VHS06_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HEX2988505.1"
                     /translation="MELQELSTGTPVSYDFDRLIERRGTDSVKWGRYGADVLPLWVAD
                     MDFPSCAPVISALRERVEHGVFGYGMEPPELRGVVQERLEQKYGWKVRPDSMMFLPGV
                     VVGLNLAVHACAGVGDELLLQTPVYPPFLYVGKNTGRGTVNSPLVRGQKRYEIDFEAF
                     EAAITPRTRVFLLCNPHNPVGRVFTRPELERIAEICLRHDLIICSDEIHQDFVYEGHV
                     HCPIASLSPEVAARTVTLISPSKSYNIAGFHFAIAVAENPEVKARLEAACAGLLASRP
                     GLLDFVAGLAAYQHGGEWLRQVVSYLQANRDYLVSFVERELPGVNMVEPEGSYLGWLD
                     CREAGIDGSAMEFFLKEAKVALNPGPDFGKGGEGFVRLNFGCPRSILEEALSRMRSAL
                     VRG"
     gene            complement(5986..7038)
                     /locus_tag="VHS06_10090"
     CDS             complement(5986..7038)
                     /locus_tag="VHS06_10090"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2988506.1"
                     /translation="MKAIAKTKPTEPGVNVVEAPAPTISAPDDVLVKVQTTSISGSEI
                     NIWRGTYRRPNGEPVAPGRVLGYEFAGIVEKAGAAAKEAGFLPGRRVALGSPFVPCTK
                     CEPCSMGFVNRCRNWGHVGITRDGSDAELACVPTITLQLLDDKVDSLDAAFLNTAGLA
                     VRAVDRSHLVPGDRIAVVGPGPVGLFMLQAARAAGAAWAGVVGLPSDASRLKIAAQLG
                     ADACFTASDSTVAEIKAATEGLGPEVVFEAAGSPEGIKMAVDLVRVGGTVILAGLPPQ
                     KIAPFESIRVARDEISMIGAEGNLPADRRRALRLFASGDLKARPLVTHRFSMDKAEEA
                     FQVAASGAACKVVFDI"
     gene            7650..8042
                     /locus_tag="VHS06_10095"
     CDS             7650..8042
                     /locus_tag="VHS06_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2988507.1"
                     /translation="MAGTIDYTLEHLHVYCSDMAASERWFVSGMGAELVRHLEIKGFP
                     ATELRIGGARLLLRGQRQGEVLGPGGTGRCGWDHLGLLVKDMDATVAELKRRGVEFEM
                     EPQPLDATTRMAFVRGPDALRIELLERS"
     gene            complement(8162..8806)
                     /locus_tag="VHS06_10100"
     CDS             complement(8162..8806)
                     /locus_tag="VHS06_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988508.1"
                     /translation="MAEKKSRKRDRRRKGGKYPGNAELGVLGVAATGEASPRQDYGEG
                     ALGRPLEGTITQSDAVREGSTGASFGEASTGGGTVRPGSSGGGPTGLGAQGSSYDVGV
                     RGPGTVGGARARLEHWQGPLPGLRARWRQGAASREIPREEYEPAFSLGWEMASEPRHR
                     GRDWEEVVADFRREWARRHPDIPWERVAPAARDGFEESPETAEVRGEAEHRRAE"
     gene            complement(8916..10376)
                     /locus_tag="VHS06_10105"
     CDS             complement(8916..10376)
                     /locus_tag="VHS06_10105"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX2988509.1"
                     /translation="MTASLQERLVRIDEYRWEIPVDYKPGMRVPGLVYADDRLMKQIK
                     EERALEQVANVAFLPGIVWRSLAMPDIHWGYGFPIGGVAATRVEDGVISPGGIGFDIN
                     CGVRLLRTDLTEKEVRPRLPDLIQELFRAVPSGVGAEGRIRLGSLELDGVLKMGARWA
                     VEAGYGTPADLEASEEQGELASADPSKVSERARKRGSPQLGTLGSGNHFLEIQEVEEI
                     FDPTLASGMGIDSVGQVTVMIHCGSRGLGHQVCEEHLRVMVAAGKKYGIEVPDRQLAC
                     APVRSPEGQDYLAAMAASANFAWANRQCIAHWTREAFHRVFRASPEALGMEQVYDVAH
                     NVAKTEEYEIDGARRMLCIHRKGATRAFPPGHPDLPARYRSVGQPVLIPGDMGRYSYL
                     AAGTQRAMEESFGSTCHGAGRVMSRGEAKRTMSAQQLLSQLAAGGVQVQTADRGTLVE
                     EAPQAYKDVEEVVHVVAGAGLVKKVARMRPMGVVKG"
     gene            complement(10446..>10816)
                     /locus_tag="VHS06_10110"
     CDS             complement(10446..>10816)
                     /locus_tag="VHS06_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX2988510.1"
                     /translation="AWGRDLPHTFSSAARAMFEIIADLSNVSEKESREVELTSAEPDT
                     LLVDWLNELLYLFDAEQLLLCRFDVLILPGEALRATVYGERVDPARHLVKMGIKAATY
                     HMLELKCEPAGCEARVIFDV"
BASE COUNT         1786 a         3385 c         3526 g         2119 t
ORIGIN      
        1 tataggtcct gtgaccggtg tcgcgatcct gatcccgatc acatttggga tgaacccgac
       61 gacggcgatg atcatgctgg cggggattta ctacggggcg atgtacggag gctcgacgac
      121 atcgatcctc gtaaacgtcc ccggcgagtc ggccagcgtg atgactgccg tcgacggata
      181 tcagatggcg cggcaagggc gagcaggggc tgcccttggg atgagcgcca tcggcagttt
      241 tgtggccggt acgttctctg tggtaggact gatggttgtg gccggtccga tcgtgagcct
      301 tgccctctcc ttcggaccgc ccgaatactt cgccctgatg ctgctgggct tgagcaccct
      361 gacgaggctt gcgggcaaga gcctgctgaa ggggcttgcc atgggcttcg cgggaatgct
      421 gatcgggacg gtgggcctag acccgatcgc tggggtggcg cgcttcacct tcgggcggcc
      481 ggacctgatg gatgggatcg ggttcgtatc cgtcgccatg gggctatttg ccttcggaga
      541 gatcctgacg aacctcgagg ggtcgctgga tagggagatc ttcagctcca agattgggag
      601 cgtcctgccg acgatgcagg actggatcga ctgcaagtgg acactgctga ggagcacgtt
      661 gatcggcttc gtgatcggct ccctgcccgg cgccggagcc acggtggcgg ccttcatggc
      721 gtacgcggtg gagaagaagg catccaagca ccccgaacgg tttggaaatg gggccatcga
      781 aggggtgtgc gcctcggaaa gcgcaaacaa cgcggcgacc ggtggggcac tggtgccgct
      841 gctgacgctg gggatccctt cgtccgcgac ggtggccatc atgatgggtg ccctgatgat
      901 gtacggtctg aggccgggcc ccctgctgat ggcggagaac ccggagttct tctggggcct
      961 cgtggcgagc atgtacgtgg gcaatgcgat gctgctagtg ctgaatctgc cgctggtcgg
     1021 gatgtgggta aagattctca aggtgcccta tccggtgctg atcccgctga tcctgttgtt
     1081 cgttctgctg ggcgcgtaca cggtgggagg ggcgatcagc gacctctgga tcatgtttgc
     1141 cttcggggtt gtcggctacc tgatgaggaa gttcgactat cccgccgccc caatggtgct
     1201 ggcactggtt ctggggccga tcatggagaa gaacctgaag ctgtcgctga cgatgtcgca
     1261 aggggatctg acgatcttcg ccacgcggcc gatcccgctg atcctgtttg cgttgacggc
     1321 gctgtctttg gggtggccgg cgttggtgtc gctgctatcc cgaggcaaga ccgtggtgga
     1381 gatgcccgcc gcggaagagg tgtagggaat cgaacggtgc gagtgcagat gcttcgaagc
     1441 tgttgctgga gggtatagat ggtcgtgagc gtgggaaccg gcggggagag ggtgtccagg
     1501 ctgccccgcc tggctattgc catgggcgat ccggcgggag tcgggccgga gataatcgtg
     1561 aaggcgctgg ctgaacccga gatctacgat ttctgcgagc cggtggtgat cggggatgcg
     1621 ggggtgctga tgcgggcccc gggttggccc aacggtgagc cccgattgga tctggtagac
     1681 gatgtggagt ccgcctgccc gagggccggg cgggtttccc tcctcaatct gggaaacgtc
     1741 cctccatctt tgaatgttgg ggaggcgtca gcagaaggcg gtcgggcctg tctggagtat
     1801 ctggaaaaga tcaccaggct ggcgatagaa ggcaaggtgg acgccgtcgt gtttggccct
     1861 ctgaacaagg aggcgatgaa gaaggcgggc tccccccaca ccgacgagta cggcctcttt
     1921 gcccggatgt gcggtgttga cgactactcg gtgttgatgg tcgggccgca ttttaccctg
     1981 gcctccgtga ccctgcacct gcccttcagc gaagtctccc ggcacatcac gaaggagagg
     2041 gtcctctcca ccattcgcca tggggagcga gcggcgcagg ctgccggggt ggagcatccg
     2101 cgcatcggcg tcgcggcact gaacccccac gcgggcgagg gcggcacgct tggcaaggag
     2161 gagcaggatg tgatcggccc tgccgtggcg gccgccgtcg cggagggcca tgatgtcgag
     2221 ggcccattcc ccgcggatac cttctttatg accaccaggg aaccccgcta cgacgtctac
     2281 gtcgggatgt atcacgatca gggccggatc gccctgaagt tgttggattt cgggcgggtt
     2341 accaccatgg ccgagggttt gccggccttc ttctgcaccg tcggccatgg cactgcctac
     2401 gacatcgccg gcaagggtat cgcccaccac gagaacatga gggataccct gttgcttgca
     2461 gccaggaggg cggctgccca tcgtcgagga aagtagccgt ttgccgtgcc gcagtctgga
     2521 cagccgtacc agccgagcgt aacctttgct tcagcaactt gggagctacg tcgacgggga
     2581 ggggttcggt atgaggctgg aagggaaagt tgcgctcgta accggtgccg gcagcgggat
     2641 cggccgtgcc atagccgttc gtcttgccgg tgatggtgcc aggatcgtgg ccgcggatct
     2701 caatccggag ggggccacgg agactatctc gaagatcgag gcggcgggcg ggagcgggag
     2761 tgccgttcag gtggacgtcc gggatgtatc ctcgctgcaa gccatggtcg atgggacggt
     2821 ggagcggttt ggacggatcg acatcctggt ggcgtgtgcg ggtatcgttc aaacgaagcg
     2881 gatgcgcgac ataacgcccg aggattggga tcggatcttc agtgtgaatt cccgggggct
     2941 cttcttcacc atgcagttgg tgtcgaagca aatggagcgt caggggagtg gggtgatcct
     3001 gaatatgtcc tccgctgctg ctcgcggccc tcgccccatg tacacccact acgccgccag
     3061 caaggcggct gtcatctcca tcacccggtc tgccgctgca tcccttgccc cttcgggcgt
     3121 ccgggtcaat gccatctgtc cgggtatcgt ggagactccc atgtgggacc agatcgacag
     3181 ggagtacgcc gcggagtctg ggaggcccct gggcgaacac agacgccagc gggcgaagga
     3241 gatcccgctg gggcgattgg agacggcgga ggacgtggcg tcggctgccg ctttcctcgt
     3301 gtccgacgat gcctcctata tcaccggtca gactctgaac gtcgacggtg ggataaacat
     3361 ggactgaggg tagggggaca ggcactaccc cttcgatagc ggagtggtgc ctgtcccgtt
     3421 cctcttcaga cccgcaaaga ggccggagag ccgctccccc tgccgtggct ccgggatcgc
     3481 cagggcgaag ctctctcggc tgaacccctg cttgactacc tgctccggca ccttgaagaa
     3541 gaggttatcc gggctcagct gggtgcggtg gcagtggagg gcggtccact tcgcctcgat
     3601 gaactccgag atgtccatgg tgacgttgat cttctcgtcg ggccagccgg cgccggcctc
     3661 ctcgaagcga gcgaagtcgc tggtgtcgat ccccatctcc tgcaatcggt cgcgcatctc
     3721 gatgaagaag gaccgaggga tggcggtgta gaacagccga gacggctgcc aggccgcccc
     3781 ccgctccgga aaccgctttg gatcgccggc cgcgacgaag gcggcagcgg tgtgccggct
     3841 gatcgcgatg tggtccgggt ggccgtagcc gccgcccggc tcgaatgtta ctaccacctt
     3901 cggcttcaga tcgcgcatga tgcccaccag ttgggagacc acatcctcgt cccgagcttt
     3961 ggcaaaggcc ttcgggttct cgttgtctgc cgtgccagcc atgccggagt cgcgataccc
     4021 gaggaagatc acgtcgctgg cacccagttg ccgggccgag cagcgcagtt cctcgcggcg
     4081 cacctccccc aggttctcgg gcgaggccag ggcagggtcg ctgatctccc ccacctctcc
     4141 ggccgtggcg cacacgagga ccacgcgcgc accttccgag gcgtagcgag cgagcgtgcc
     4201 gccgcttccg aaagcctcgt cgtccgggtg ggcgaagcag gccagcaggg tttcgcccgc
     4261 ggctgtgtcg ttcacgttgc gttgagtgct gtctaccact tcaacctcca ctttcatcct
     4321 ccctgagggt agcactgggg agggaaagca tcaaccgttc cacttgttga acctcgtggt
     4381 tgggggcaga tgcccgcacc tgttcccgta gtgggcacgc acccgtctag cacacgaccg
     4441 cagtgatcgt gacctgtagg ggcgacgcat gcgtcgccct cgggcgacat atatgtcgcc
     4501 cctacccatc gaattcggcg tggtcgtgtt gtactaggag catgcgtcgc cccacagaag
     4561 actcagtgag taggtttgcg aaccggccgg atggagaatt acactggcac ggttggcgcg
     4621 atctctttcc cgcaccgcat ctctttgagg aggcctctct tggaactgca agaactctcc
     4681 acaggcacac ccgtgagcta cgacttcgac cgtcttatcg aacgcagagg cacagacagc
     4741 gtgaagtggg gccgttatgg ggccgatgtg ctgcccctct gggttgcgga tatggacttt
     4801 ccctcctgcg ccccggtgat ctccgctttg cgggagaggg tcgaacatgg ggtgttcggc
     4861 tacgggatgg agccgccgga gttgagaggt gtggtccagg agaggctgga acagaagtac
     4921 ggctggaagg tgcgcccaga ttccatgatg tttctgccgg gcgtggtggt cggactgaac
     4981 ctggcggtcc atgcctgcgc cggggtgggg gatgaactgc tcctgcagac gcctgtttac
     5041 ccacccttcc tatacgtcgg caagaacacc ggccgtggca cggtgaactc tcccctggtg
     5101 cgcggacaga agcgctacga gatcgatttc gaagccttcg aggcggccat cacccctaga
     5161 accagggtct tcctgctgtg caatccccat aacccggttg ggagggtctt caccagacct
     5221 gagctggagc ggatcgcgga gatctgtctg cgccacgacc tgatcatctg ctccgacgag
     5281 atccaccagg acttcgtcta cgaggggcat gtccactgcc ccatcgcctc gctctcccct
     5341 gaggttgccg ctcgaacggt gacgctgatc tctcccagca agtcgtacaa cattgccggc
     5401 ttccacttcg ccatcgcagt ggcggagaac cccgaggtga aggcgcggct ggaggcggcg
     5461 tgtgcgggac tgttggcctc caggccgggg ttgctggact tcgtcgccgg attggccgcc
     5521 taccagcacg gtggggaatg gctgcggcag gttgtgagct atctgcaggc caaccgggac
     5581 tacctggtga gcttcgtgga gagggagttg ccgggggtga acatggtcga gcctgagggc
     5641 agctacctgg ggtggctgga ctgccgagaa gcaggaatcg acgggtctgc catggagttc
     5701 ttcctgaagg aggcgaaggt ggcactcaac cccggtcccg attttgggaa ggggggagag
     5761 ggcttcgtac ggctcaactt cggctgccct cggtcgatcc tggaggaggc gctgtcgcgg
     5821 atgaggtcgg cgctggtgcg agggtagcga agaggcagct cagctcaccg gacttcgtac
     5881 accatgttga accgtgagca tcgcccattg ggtagcataa ccgggaagtt ggatggtgcc
     5941 acgccttccc cacctcagta tagggaaggc gtggcaccat cgctactaga tgtcgaacac
     6001 tactttgcag gcggctccgg aggctgccac ttggaatgcc tcttcggcct tgtccatgga
     6061 gaagcgatgg gtgaccagcg ggcgggcctt caaatcaccg ctggcgaaca gccggagagc
     6121 cctgcggcgg tcggcaggaa ggttgccctc tgcaccgatc atggagatct cgtccctcgc
     6181 tacccgtatt gactcgaacg gagctatctt ctggggcggg agcccggcga ggatgacggt
     6241 tccacctacg cgcacgaggt ccaccgccat ctttattccc tcgggactgc cggccgcctc
     6301 gaagacgacc tcgggaccga gcccttcggt ggcagccttg atctccgcga cggtggagtc
     6361 ggaagcggtg aagcaggcat ccgctcccag ttgagccgcg atcttcagac gggacgcatc
     6421 cgaaggaagg ccgacgacgc ccgcccaggc ggctcctgca gccctggcag cctgcagcat
     6481 aaacaggccg accgggcccg ggcccaccac cgcgatcctg tcaccgggta ccagatggga
     6541 tcgatccact gccctcaccg caagccctgc ggtgttcagg aaagccgcgt ccagcgagtc
     6601 taccttatcg tccaggagtt ggagcgttat ggttgggaca catgccagtt cagcgtcgga
     6661 accgtcccga gttatgccga cgtgtcccca gttccggcag cggttcacga atcccatgct
     6721 gcacggctcg cacttggtgc aggggacgaa ggggcttccc agggccaccc gccgccctgg
     6781 taggaatccg gcttccttag ccgctgcccc cgccttttcc acgatgcccg cgaactcgta
     6841 gcccagcacc ctccccgggg caaccggctc tccgttgggc ctccggtagg tacctcgcca
     6901 gatgttgatc tctgagccgg agatgctggt ggtctgcacc ttgaccagaa catcatccgg
     6961 tgcactgata gtgggagccg gggcctcaac tacgttgact ccgggctccg tgggtttggt
     7021 cttggcgatg gctttcatct ctctccacct ctgtgatgcg aaaatctatt tcgcaaccgc
     7081 caggactccg agggtgtagc attccttctg cgcatcctct cggggactcc gaaatggctt
     7141 ccctcctcgc ttctcccgat agcgcgggtc aagggatcct tatgaacctc aatagtcgca
     7201 gtgcgtttgg tgggattgac ccgcaggagt tggcctttgt aaccgaagag catgcatacc
     7261 tccagcagaa gacctggcgg cgctcatggt attcgtcgct ggcgcgggat cgcgggcttg
     7321 ttgagggacc ggctgcgagg accgacgaat gatcctcaaa caggatatcg gcagtatctc
     7381 agtgtagtag ttgaatgtca atgtttaata tatcaatcca tgcaacgttg gtcagcgatg
     7441 gggtgacggg gcggctgctc ggggcccgga gcactgcctg actctctcag aggtgatggg
     7501 ggccgtcatg cgagatgggg cgtggcaatc ctcccttcct ctcgaagcga cgagggttgc
     7561 agctttctgc agttcgagca gtagctttgg agcggcgacg tgttagaatg cctccggctt
     7621 ctgatccatc tgcttgtagg agggttcaat tggccggcac tatcgactac accctggagc
     7681 accttcacgt ctactgctcc gacatggcgg cctccgagcg ctggttcgta tcgggcatgg
     7741 gtgctgagct tgtccggcat ctggagatca agggctttcc ggcgaccgag ctgaggatcg
     7801 gcggcgccag gctgttgctg cgcggccagc gacaaggcga ggtgctgggg cccggcggga
     7861 ccgggcgatg cggctgggac cacctgggtc tcctggtgaa ggacatggat gcgaccgtcg
     7921 ccgagttgaa gcgccgcggc gtggagttcg agatggagcc ccagccactg gacgcaacca
     7981 cccgcatggc ctttgtccgg ggaccggatg cgttgcggat cgagctcctg gagcgatcgt
     8041 aacaggagcg tacactcagg cggggcggct ccctctccat ttccgccccg ccggtttcac
     8101 ctgcttctcg ctggaaatcc tcgcgttgtg gcgtagtttg tgctcgggga ccgaacaggg
     8161 gctactccgc ccgcctgtgc tccgcttctc ctcgcacctc ggcggtttcc ggagactcct
     8221 cgaaaccgtc cctcgcggcc ggcgcaaccc tctcccatgg tatgtcgggg tgtcggcgcg
     8281 cccattcccg acgaaaatcg gccactactt cttcccaatc tcgccccctg tgccggggtt
     8341 cggaggccat ttcccaaccg aggctgaatg cgggctcgta ttcctccctg gggatttctc
     8401 tcgaggcagc gccctgccgc caccgagcac gaagcccggg cagagggccc tgccagtgct
     8461 ccagcctggc cctggcgcca ccgacggttc cgggaccccg gaccccgaca tcgtagctgc
     8521 taccctgggc tcccagcccg gtggggccgc cgcccgacga gcccggcctt acagtcccgc
     8581 caccggtgga tgcttccccg aagctggcgc cggtgctccc ctctcgcacg gcgtcgctct
     8641 gtgtgattgt cccctccaga ggcctaccca acgccccttc gccgtagtcc tggcggggtg
     8701 acgcttcacc tgtcgcggca acccccagca cacccagttc ggcgtttccg ggatacttgc
     8761 cgccttttcg ccgacggtcc cgctttctcg atttcttctc tgccatcagc tttctcctgg
     8821 ggcggcccgt cgaatgctcg gcgctgttcg gtggacagac gactccgttc acgaggcgca
     8881 ttcgggatgc cagcggttgg ggcgcttccc tcaccctaac ctttcactac tcccatcggc
     8941 cgcattctcg ccaccttctt gaccaggccc gcgccggcca ccacgtgaac cacctcttcc
     9001 acatccttgt acgcctgggg cgcctcctcc acgagggtgc ctctgtccgc ggtctgaacc
     9061 tgaacgccgc cggccgccag ttggctcaag agctgttgtg cggacatcgt gcgcttcgcc
     9121 tcgccgcggc tcatcaccct gccggctccg tggcaggtgg aaccgaacga ctcctccatc
     9181 gctcgctgag ttcccgcggc aaggtaggag tatcgcccca tgtcgcccgg gatcagcacc
     9241 ggctggccga cgctccgata gcgcgcgggc agatcgggat gccccggagg gaaggccctg
     9301 gtcgcaccct ttcggtggat gcacagcatc ctgcgcgctc cgtctatctc gtactcctcg
     9361 gtctttgcga cgttgtgggc gacgtcgtat acctgctcca tccccagggc ctcgggcgag
     9421 gctctgaaca ccctgtggaa tgcctcccgg gtccagtggg cgatgcactg tcggttcgcc
     9481 catgcgaagt tggccgacgc tgccatcgcc gccaggtagt cctgcccctc cggcgagcgc
     9541 accggggcac aggcaagctg gcggtccggg acctcgatcc cgtacttctt cccagccgcc
     9601 accatgacgc gcaggtgctc ctcgcacacc tggtggccca acccgcggga gccgcagtgg
     9661 atcatcacgg tcacctggcc gacggagtct attcccatcc cgcttgccag cgtcggatcg
     9721 aagatctcct ccacctcctg tatctccagg aagtggttgc cggagccgag tgtccccagt
     9781 tggggggagc ccctcttgcg agcccgctcc gagaccttag acgggtcggc gctcgccagc
     9841 tcaccctgct cctccgatgc ctccagatcg gccggcgtcc cgtatcctgc ttccaccgcc
     9901 cagcgagcgc ccatcttcag tacgccgtcc aactccagcg atcccaggcg gatcctcccc
     9961 tccgccccga cgccggaggg cacggcccgg aacagctcct ggatgaggtc gggcagcctg
    10021 gggcgaacct ccttctcagt gagatccgtg cgcagcaacc gcactccgca gttgatgtcg
    10081 aagccgatgc cgccggggct gatcacgccg tcctccaccc tggtcgccgc gacgccgccg
    10141 atggggaagc cgtaccccca gtggatgtcc ggcatagcca gcgatcgcca gacgatcccc
    10201 ggcaggaagg ccacgttggc aacctgctcc agcgccctct cttccttgat ttgcttcatc
    10261 agcctgtcgt cggcgtagac cagacccggc actcgcatcc cgggtttgta gtcgacgggg
    10321 atctcccagc ggtattcgtc gatgcggacc agacgttcct ggagagatgc agtcatcgtc
    10381 ttacctccag tatggagaca acttagggag atggcgggac acgcttggca acagttggtg
    10441 gagccctaca cgtcgaagat caccctggcc tcgcaccccg caggctcgca cttcagctcc
    10501 agcatgtggt aggtggcggc cttgataccc atcttcacca gatggcgtgc agggtccacc
    10561 ctctcgccgt acactgttgc cctcaacgcc tccccaggga gtatgaggac gtcgaatcgg
    10621 cataggagca gctgttcggc gtcgaatagg tagagcagct cgttcagcca gtccaccagg
    10681 agggtgtccg gctcggcact cgtcagctct acctccctgg attccttttc gcttacgttg
    10741 ctcaggtcgg cgattatctc gaacatcgcc cgggcggcgg atgagaaggt gtgcggcagg
    10801 tcccggcccc acgcca
//
LOCUS       DATALS010000457         6251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_349315, whole genome shotgun sequence.
ACCESSION   DATALS010000457 DATALS010000000
VERSION     DATALS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349315"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VHS06_10115"
     CDS             <1..254
                     /locus_tag="VHS06_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX2988511.1"
                     /translation="QVLGEKSYPDLTSVPEKVDVVDIFRRSEDVPPIVEQAIQVGAKA
                     IWMQEGVVSEVAAARAREAGLGVVMDRCMLKEHRQLSPQ"
     gene            380..628
                     /locus_tag="VHS06_10120"
     CDS             380..628
                     /locus_tag="VHS06_10120"
                     /inference="COORDINATES: protein motif:HMM:NF018965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1462 family protein"
                     /protein_id="HEX2988512.1"
                     /translation="MTQVVAWYLSREYGNSVQVHYNDLSQAEVRQRFPSVVEKLKKGE
                     VILPAVYVDDEIVNLGYVDYFKIAKAIEQSRGKSQASS"
     gene            963..1874
                     /locus_tag="VHS06_10125"
     CDS             963..1874
                     /locus_tag="VHS06_10125"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2988513.1"
                     /translation="MVYEVAIVGAGPAGLSAAVYTARNGLKTLVLGDPDKSRVNLSKH
                     FDNYLGFPEGISGKELMERSVAQAVRFGAEIVRGEVVGLLPGDPFKLELAKGETYEAR
                     AVILATGVAGKPSGIKGEEELIGQGVSHCVVCDGFFYRDRKVAVIGNANYAAKEAIEL
                     LAYSRDVTIFSNGRLFSIDDGLRQRLDQEGIALKDDRIAEFTHSGNGLEGIKLRNGEL
                     FPAEGVFIALGTAGSADFARTMGIELDGNSIVIDREGRTSFPGIYAAGDCTVGPQQMA
                     RAVGEGCVAALSVIAQLQGLEKPPTLW"
     gene            complement(1977..2285)
                     /locus_tag="VHS06_10130"
     CDS             complement(1977..2285)
                     /locus_tag="VHS06_10130"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEX2988514.1"
                     /translation="MRRFSNIHNLVTLFAALTILDIASTYIALQLGFVEMNRLAAALL
                     AIGGEAMMFLFKAVMSLLVIAMVIHLSPRYARLSYGLRAGNGILGLIVLSNVVQLITA
                     "
     gene            complement(2404..2673)
                     /locus_tag="VHS06_10135"
     CDS             complement(2404..2673)
                     /locus_tag="VHS06_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HEX2988515.1"
                     /translation="MIFTLEDLARYHGRDGAPAYIAYRGVVYDLSGSYQWRNGKHQVT
                     HLAGRDLTGGLKDAPHGEDLLDRFPILGKVAEVASAEPVEGPSRL"
     gene            complement(2754..3305)
                     /gene="dps"
                     /locus_tag="VHS06_10140"
     CDS             complement(2754..3305)
                     /gene="dps"
                     /locus_tag="VHS06_10140"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HEX2988516.1"
                     /translation="MARVARETVERAGIDVGELLELLVNNASAELTTYYYYTILRTNL
                     IGLQGEGIKEIAETARIEDRNHFEALVPRIYELGGKLPENMKDFHDRSACPPASLPVD
                     PTDTNAMLHVLVEAERCAVRGYTNICNLTAGKDHRTYDLSMAILNEEIEHESWFSEFL
                     GEGPSGHFMRRGETSPFVSKFLR"
     gene            complement(3683..4036)
                     /locus_tag="VHS06_10145"
     CDS             complement(3683..4036)
                     /locus_tag="VHS06_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009350244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX2988517.1"
                     /translation="MGFMGLGPGELFLILVLAVLVFGPAKLPEIGNSLGKGIREFRKA
                     TNEVMEPVNEVKKSVNEIKELPNSLLTGTPAGGGTRADAAQPARPAAAEAPVHPTTAE
                     SSTPAAEPPAKTDEA"
     gene            complement(4298..5602)
                     /locus_tag="VHS06_10150"
     CDS             complement(4298..5602)
                     /locus_tag="VHS06_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX2988518.1"
                     /translation="MRNYCLTVAQGEVKRYMVGYLAIRRSMFSVARYGTLSGVLLAVA
                     FWLLVHPQTAEAGRFGPPWQAQVSVNQTVVYSQPDAASQPVGPLGRDAIVVVTDQVKG
                     TDGGDWTKIPDGYVCSGDLSEMYTPWTAEVTAPSVSVYAYPFGTSAIRRTARQGALLR
                     VTGVSAGLEGDTNLWWATTEGYVPVGTLKWATNDWAGWWQLPDPSEATNGWWGVVVSQ
                     ANVRAASSSEAPIVGTFAGGEHVKVLAEEEGSAVGGNSVWYRIDGGRFAGGRVHSSLI
                     RRLPDPQPNVAPLPAKYQSNSWIVVDRTHSTLTFVQDGQPKFVTYVSLGLAGVATPTG
                     TYSTFGKFIGDRMSSRSVKDPTHPYDLPNVPFTQYYLDGGYGIHGTYWHDGFGSAESQ
                     GCINLTWADSAYLFRLTSPKVSDGFIYAWAGADGATPVVILN"
     gene            complement(5657..>6251)
                     /locus_tag="VHS06_10155"
     CDS             complement(5657..>6251)
                     /locus_tag="VHS06_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988519.1"
                     /translation="NLMDDGLMPLRRANFSTFPPAEERLIQEAPSPSDADYGDKATAF
                     LRREVPDRWEGMEVGFLRSYLSMVQLGDAFPEGYGDPELLPLLNLELWGLPTSRPARD
                     PNNANFVYQRFQRGILHYDVSTGTTQGLLLGEYFKSIMTGVGLPDDLAAEVGGTHFYR
                     QYDRTCPGHVARPADLPYTTLVGAFEMDVPQEILGVE"
BASE COUNT         1273 a         1853 c         1986 g         1139 t
ORIGIN      
        1 accaggtgct cggggagaaa tcctacccgg atctgacatc ggtgccggaa aaggttgacg
       61 tggtggacat ctttcgacgc tccgaggacg tcccgccgat cgtagagcag gcgatccagg
      121 tcggcgccaa agccatctgg atgcaagagg gagtggtcag cgaggtcgct gcggccagag
      181 cccgggaggc aggcctcggc gtggtaatgg atcgttgcat gctcaaagaa cacaggcaat
      241 tgtcgccgca gtagtcacag gggaggaaaa ttcgatggag gcaaacggga aagccgtaaa
      301 cgtcgtcaac gtcttcgacg acgcggccag cgcccgcttc tgcagcagcg gttgagggcc
      361 cccacaatct caggaagaga ttacgcaagt cgtcgcctgg tatctatcca gggaatatgg
      421 caacagtgtc caggtacact acaacgacct gtctcaggcc gaggtgcgac agcgcttccc
      481 ttcggttgtg gagaagctga agaagggcga agtgatcctt ccggctgtgt acgtggacga
      541 tgagatagtg aacctgggct acgtggacta cttcaagatc gccaaggcca tcgagcagtc
      601 ccggggaaag agccaggcaa gcagctagta gacggacgcg gagacgcggg gacacggaga
      661 cgcggagagg gcttgttatc gtctccattt cccctccact cgcttcggtt cagtcccgat
      721 tagggagggg agaaaccatg gagatggcct tcgccatagc gctgttccta atcctggcgc
      781 tgctgatgtc caggggcagc ggttccggta agggttgagg catctagagc tccgcggagc
      841 ggggcacaag ttgggctcgt gtggtccact gaaccgggtg ccatattgcg ggagtctggt
      901 ggtgaactgg gctcccgtac actttcctgc agcatcaccg acggatcgcc gcggaggaga
      961 gaatggtcta cgaagtcgca atagttgggg ccggacccgc cggactttcg gcggccgtgt
     1021 acactgcacg gaacgggctg aaaactctgg tgttgggcga ccccgacaag tcgagggtga
     1081 acctcagtaa gcatttcgac aactacctcg ggtttcccga gggcatatcc ggcaaggaac
     1141 tgatggagcg aagcgtggcc caggcagtcc gcttcggagc cgagatagtc cgcggggagg
     1201 tggtgggtct gctgcccggc gatcccttca agctggaact cgcgaagggg gaaacgtacg
     1261 aagcccgagc ggtcattctg gccaccggcg tcgccggaaa gcccagcggg ataaagggcg
     1321 aggaggagct aatcggccag ggggtttccc actgcgtggt gtgtgacggc ttcttctacc
     1381 gcgaccgcaa ggtggcggta atcggcaacg ccaactacgc ggccaaggag gcgatcgagc
     1441 ttctggccta ctcccgggac gtgaccatct tctccaacgg ccgcctattc tccatcgacg
     1501 atggccttcg gcagcggctg gaccaggagg ggatcgccct caaggacgac cgcatcgcgg
     1561 agttcaccca cagcggcaac ggactggaag ggatcaagct gcgcaacggc gagctgttcc
     1621 cggccgaggg ggtcttcatt gcgctgggca cagccgggtc ggccgatttc gcccgaacca
     1681 tggggatcga gttggacggc aactcgatcg tgatcgacag ggagggccgg acgagcttcc
     1741 ccggcatata cgccgcggga gactgtacgg tcggcccaca gcagatggcc agggcggtcg
     1801 gagagggctg tgtggcagcg ctatccgtca ttgcccaact gcaggggctg gagaagccgc
     1861 ctacgctgtg gtaggggcag agcagccgcc aattcgacac aaagcacaag gtcatgtagg
     1921 caggcccgag tcgttcgcga ctcgggcttt tcttcttgag agacgggacc ggcgcactac
     1981 gccgtgataa gttgcactac gttggagagg acgatcagac cgaggatgcc gttgccggct
     2041 cgaaggccgt aggaaagcct ggcatagcgg gggctgagat ggatcaccat cgcgattacc
     2101 aggagggaca tcacggcctt gaacaagaac atcatcgcct caccaccaat cgccaacaaa
     2161 gcggcggcca gccggttcat ctcaacgaaa ccgagctgta gggcgatgta ggtggaggct
     2221 atgtccagaa tggtcagggc cgcaaagagg gtcacgaggt tgtgtatgtt ggaaaatcga
     2281 cgcatcggga agtcctcctt ctcggtgctg ggcgtctcga gcccgacaag ccgccccgct
     2341 tctaccttct ggaggagcag tatagcacaa atgggctatt gaattgccat agattttctg
     2401 catctacagc ctcgaaggtc cttcgaccgg ctcggccgat gcaacctccg ccaccttccc
     2461 gagtataggg aaccgatcga gcaagtcctc accatggggc gcatccttca ggccgcccgt
     2521 aagatctcga ccggcaaggt gggtcacctg gtgcttgccg ttccgccact ggtagctgcc
     2581 cgaaagatcg tagaccactc cgcgataggc gatgtacgcc ggagcgccgt cccttccgtg
     2641 atatcgggct agatcctcca gggtgaagat catctcctcc acggccgctc caacccagcc
     2701 ctgtttgggg agtcaggtcg gccggatagc ggtccccctg gctccccaga tgcctatcgc
     2761 aagaatttcg aaacgaaggg cgatgtctca cctcggcgca tgaaatggcc ggatggcccc
     2821 tcgccgagga actcggagaa ccaggactcg tgctcgatct cttcgttgag gatcgccatc
     2881 gataggtcat aggtccggtg atccttaccg gcagtaaggt tgcagatgtt ggtgtatccg
     2941 cgaacggcgc acctttcggc ctccaccagc acgtgcagca tcgcgttggt gtccgttgga
     3001 tcgacgggca ggctcgcggg gggacaggcc gagcgatcgt ggaaatcctt catgttctcg
     3061 ggaagcttcc cgcccaactc gtagatgcgg ggcacgaggg cctcgaagtg gttccggtcc
     3121 tcgatgcggg cggtctcggc gatctccttg atgccctcac cctgaaggcc gatgagattc
     3181 gttcgcagga tggtgtagta gtagtaggtt gtcagttccg ctgaggcgtt gttgaccagc
     3241 agctccagca actcgcctac gtcgatgccc gccctctcaa ccgtctcgcg tgctactctc
     3301 gccattgcca gatcctttcc gtctgtcatt acttcgccac aaccgatcga cgctgggctc
     3361 agatggtgga ctaccgtgcc accggctcga gtacaaagaa cctgtgaagt cgcactgcgc
     3421 ctccgagatg gcgctgccgg gaggcgtcct atctaccgcg ttccagaaga agcgcccgca
     3481 ataatcgaac cagccgaatg gttctcatgc cgtggagagg acacggggcg gctatcgctg
     3541 cccaatcggg cgccatcgtg ctcacgagca ggagcgagag ggggtactcc tgaggggatc
     3601 gactacgggg aagtgcagcg accgggcggt ccaaggcggg ccacccggtt cagagaacag
     3661 tacggaaaga gatggagaag agctacgcct cgtcggtctt ggcgggcggt tcggcagccg
     3721 gcgtcgagct ctcggccgtc gtggggtgta ctggtgcctc agcggccgcg ggacgggcgg
     3781 gttgagcggc atcggccctg gtgcctccac cggcgggggt tccggtcaag agcgagttgg
     3841 gaagctcctt gatctcgttg accgacttct tcacctcgtt cacgggctcc atcacctcgt
     3901 tggtcgcctt ccgaaactcc cttatgccct tgccgagtga gttgccgatc tccggcaact
     3961 tagcaggacc gaagacgaga acggccagca ccaatatgag gaacagctca ccaggaccca
     4021 agcccatgaa acccatttgc ggcctccaat tctcccccag agggtgggga cctcatgttg
     4081 tcagatcacc tactgccagt caagagggtg tttcggagcc gccggaacgt cacttcccga
     4141 cagaaagggg aaaccgggct atccgtcccc cgatagatag cccggttgga gaatgggaaa
     4201 cgaagcgtct tccctacgcg tgttttacca ttctggtccc atgcaagcct gaggaaatgc
     4261 ttaaggaatc ctgcaccgaa actgaggatt catagagtca attcaggatg accacagggg
     4321 tcgccccatc ggcccccgcc caggcgtaga tgaagccgtc cgacacctta gggctggtaa
     4381 gcctgaacag gtaggcactg tccgcccagg tgagattgat gcagccctgg ctctccgcgg
     4441 acccaaaacc gtcgtgccaa taggtgccgt ggatcccgta gccgccgtcc aggtagtact
     4501 gtgtgaaagg aacgttgggc agatcgtagg ggtgggtggg atccttgacc gaccggctgg
     4561 acatccggtc gccgatgaac ttaccgaagg tgctgtaggt gccggtgggg gttgcgacgc
     4621 cggccaaccc cagcgaaacg taggtgacga acttcggctg gccgtcctga acgaaggtca
     4681 gcgtggagtg ggtgcgatcc acgacgatcc acgaattgga ttggtacttg gcgggtagcg
     4741 gcgcgacgtt gggctgcggg tccggaagcc ggcgaatcag ggaactgtgg accctgcccc
     4801 ccgcaaagcg tcctccatct atccgatacc atacggagtt gccgcccacc gccgacccct
     4861 cctcctcagc caacaccttg acgtgctcgc caccggcaaa ggtgccgact atgggcgcct
     4921 cgctgctgga ggccgcacgc acattagctt gcgaaacaac aacaccccac cagccgttgg
     4981 tcgcttcgga gggatcaggc agctgccacc aaccggccca atcgttggta gcccacttca
     5041 gcgtaccaac gggcacatat ccctcggtag tcgcccacca gaggttggta tcaccctcta
     5101 ggcctgccga cacgccggtt accctaagca gagccccctg ccgtgcggtg cgcctgatag
     5161 cgctggtgcc aaagggatag gcgtacacgg agacagaggg agcggttacc tcggcagtcc
     5221 agggggtgta catctcgctc agatcgccgg aacagacgta gccatccggg atcttcgtcc
     5281 aatcgccacc gtcggtcccc ttcacttgat ccgtcacgac cactattgcg tcgcgtccca
     5341 gtggtcccac gggctgtgag gcggcatcgg gttggctgta cacaaccgtc tgattgactg
     5401 agacctgggc ctgccagggc ggaccgaagc ggccggcctc ggctgtttgg ggatgaacca
     5461 acagccagaa ggccacggct agtagcaccc cactcaaggt cccgtaccga gcaacactga
     5521 acatcgatct ccggatcgcc aggtaaccga ccatatacct tttcacctca ccttgggcaa
     5581 cagttaggca gtagttacgc atcaattggg ccgcagaaga agagacgagt cgaggggtcg
     5641 aggaggacag atgtggctat tccacgccga ggatctcttg cggcacgtcc atctcgaatg
     5701 ctccgacgag cgtagtatag ggcaggtcgg cgggacgcgc cacgtgtccg gggcaggttc
     5761 ggtcgtactg ccggtagaag tgggtgcccc cgacctcagc ggccaggtcg tccggcaggc
     5821 cgaccccggt catgatgctc ttgaagtact cccccagcag caacccctgg gtcgtgccgg
     5881 tggaaacgtc gtagtggagg atccctcgct ggaagcgctg gtaaacgaag ttggcattgt
     5941 tggggtccct ggcgggtcta ctggtgggaa ggccccacaa ctccaggttg agcaatggta
     6001 gaagctctgg atcgccgtac ccctcgggga aggcatcgcc gagctgtacc atcgacagat
     6061 agctacgcag gaagccaacc tccatccctt cccatcgatc cggaacctcc ctacggagga
     6121 aggcggtcgc cttatccccg tagtccgcat ccgacggcga tggcgcctcc tgaatcagcc
     6181 gctcctcggc aggcgggaag gtgctgaagt tagctcgcct gagaggcatc agcccgtcat
     6241 ccatcaggtt c
//
LOCUS       DATALS010000458        12628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_441339, whole genome shotgun sequence.
ACCESSION   DATALS010000458 DATALS010000000
VERSION     DATALS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 12628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12628
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441339"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VHS06_10160"
     CDS             <1..509
                     /locus_tag="VHS06_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2988520.1"
                     /translation="LAEGIRSRAESALDGWRVAAGVGRPAQSHSGLVTTYREAEGALG
                     IGIRVNGPSSTTYFGSLGILRLLAQVGNAVELQCFRAEMLGKLEDHDRKAGGELLKTL
                     EAFFQCHGNLSRTAEELSLHRNSLLYRLQRVEEISGLEMDDPEVRLSLQVALKVRQLL
                     EAERVGRG"
     gene            592..1953
                     /gene="glnA"
                     /locus_tag="VHS06_10165"
     CDS             592..1953
                     /gene="glnA"
                     /locus_tag="VHS06_10165"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX2988521.1"
                     /translation="MAKEAGKDRDKIQQILETVEREQVKFVNLQLTDIVGMVKQVTIP
                     AHELQDTLENGKWFDGSSIEGFARIHESDMYLEPDLSTFGIVPFGEGSMTTAKMTCNV
                     FMPDGEPFIGDPRFILKSALDEARSMGYEFFTGPELEFYLFHTRENGHPEPLPHDEGS
                     YFDMSTDLASDVRKEMVEALELQGIAVETSHHEVGPGQHEIDMRYDEALRMADKVVEF
                     KSTLKSVAHRHGLHATFMPKPVFGIAGNGMHTHQSLWSIAEGRNTFYDPHDQYGLSDV
                     AKHYIAGQLAHAKAMCAVLSPLINSYKRLVAGFEAPVYVSWARTNRSALIRIPKITGG
                     KFEATRVELRSPDPTCNPYLAFAVMLKAGLDGIKRELPLVQPIEEDLFEFTDEMMVRH
                     GVGVLPGSLHQALDEMEKDEVVKEALGPHAYARFLEAKTIEWKSFRQTVTSWELERYL
                     GIY"
     gene            complement(2184..3665)
                     /locus_tag="VHS06_10170"
     CDS             complement(2184..3665)
                     /locus_tag="VHS06_10170"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEX2988522.1"
                     /translation="MADPLKILFLSAEMAPFAKTGGVSEVAAALPRALRNLGHDVRVA
                     IPRHGRIDPARFSLRPVLSGVQIPVDDHQEAINVLSTELEGNLPVYFIDNEKYFAREN
                     LYGYPDDGERFIVYCRAVMEMLKLLDWKPDVIHLNDWHTAIVANWLKTIYKEDPFFRN
                     VASVYTIHNLAYQGIFGWRILEIAGVDEYSFMYPQIEEFPNVVALMAHGILFADVIST
                     VSESYAKEILTAEYGEKLHTLLNDRKDRLFGILNGVDYEAMNPATDPALAQHYDIASL
                     DKKVVNKLAFQKEANLAVDEKVPLIGCISRLTSQKGLDILGEVIDGLMDLPLQLAILG
                     TGDQYYHEMFTRVARRYPGKVAVFLTFNSAIAQKIYAGSDIFLMPSRFEPCGSNQMVA
                     MRYGSVPVVRRTGGLADTVKDFDPRTGEGTGFTFDKYSGMMLFAAIVRAMESYKHHNF
                     WRRLMETGMKADYSWESTARKYVELYHRAMEFQAQTEQPVKVG"
     gene            4000..4716
                     /locus_tag="VHS06_10175"
     CDS             4000..4716
                     /locus_tag="VHS06_10175"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2988523.1"
                     /translation="MDQLDPHYEIDRRDLSDRVYQVLRTQILSGKLAPGQRLSLDEFA
                     QYFKVSITPVRDALRQLSSDGLVELQPRRGAFVTQPTWSHVEEVYQIREILECAAVEF
                     VIRRGGEALREMEQLVEEIIGTTVGEVHSDYLAYIRLDQRFHQFLIDCVGNKKLSEIY
                     AGLRSHTLVTLALYSSADQRATDTLSEHEAILDALRRGDVVAAQSALRTHLRNAKAEI
                     ERKVVEGKLPVNGTHSTPLL"
     gene            4860..6026
                     /locus_tag="VHS06_10180"
     CDS             4860..6026
                     /locus_tag="VHS06_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2988524.1"
                     /translation="MSKTVHVGVVGAGIAAEFHLAAYRRVSGYSVEIAGITSRTPSKA
                     AALAEQFGIQRLYPDLSSLLSDREIDVVDLCVPVHLHCSMAIQAAKAGKDVVCEKPLL
                     GFAGNGRTGSVPRGEMFSAVQDELEAVEAAFRASGTKLLYAENWVYAPAFRRIAQMAE
                     SCGGKILEIRGNEAHSGSASPFSMKWETSGGGSLLRLGIHPLSAAIYLKQREGIVKQG
                     YPYRVRSAWAQMSDLTRVDGFDPATTRLVSGWVDVENWAVGVLTFDDNSVAVIHSSDI
                     SLGGVSSWMELYTTNARLRCNIHPNDTCISYSPDPRPFDGLFLNEKLETKAGWNYPSV
                     DHNWEAGYVQEIEDFVASVAEDREPLSGLELALESIRAAYALYMSAEEGVRVDL"
     gene            6205..7371
                     /locus_tag="VHS06_10185"
     CDS             6205..7371
                     /locus_tag="VHS06_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2988525.1"
                     /translation="MRRFSWLVAVFAVLGMSLGVVLLSGCDSGGPSEIKVGIIEDLSG
                     ATSAWGEGLQNGAIYAIEKINKEGGLLGKQIKYTSYDFKGNVPESLTAYNRLVDQDKV
                     VAVIGTPNSAMHIALAPLADEKKVPVIGDPMDERATTPEANKVNAYTFLGEPAAGEQA
                     RAIASYTLNEMKLKKVAILFDQGNPYSVSLATPFAEYFSKNGGQVVANEPFQSGTNDF
                     RAQLTKIKQSSPEVLHITSYLKENSLIVAQMRELGMNIPVVGNNSCFYPFAETAGKDA
                     EGVVFPNNISLDDPRYQDYISGYKARFGKDPVVHAWMGYDNMMILANAIKKANKIDSV
                     AIRDAIENQTKDVQGMTDKITIDPKTHRPVNLPLAILQVKDGKYATLKASYLPK"
     gene            7447..8328
                     /locus_tag="VHS06_10190"
     CDS             7447..8328
                     /locus_tag="VHS06_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2988526.1"
                     /translation="MYVLQQLINALSVGGVYALVAVGFALVYSILKFSNWSHGAVIML
                     SSYFGYVMASRVGLPLVPTLLLTMVAGGFLAVIIELVAFRQIRLKNGPLIYFFVTSIT
                     VSTGAQFAMYPTVGGNFFRWPEIISSTAIQMGGVTVSTLNLMMGGVCLLALVVLNLIL
                     FRTRIGTAIRAASNDVRAAALMGVDINFVIAATFFLSGALGGLAGTLMGISYTTYPEM
                     GAEAMVKGFVAAVVGGLGSISGAVMGGILLALVETVVTMTPVGSSAAPVAVFAILLVL
                     LFVRPWGIAGKATDEKA"
     gene            8335..9228
                     /locus_tag="VHS06_10195"
     CDS             8335..9228
                     /locus_tag="VHS06_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2988527.1"
                     /translation="MQYYVDLATLFLIALTAVVGVFALTGLTGLFSFGQGGFMALGAY
                     TAVLTNMRLGVPFPIALLLGVVMAVAASLLIGFPALKLRRDFFALATFGFGEAVTAIF
                     QMFTQVTGGTMGLPSIPRYTEFWMALLVAASAVYLVANFKNTKMGRQSIAIRNDELAA
                     QALGINAFRHKMIVFAFSAGLAGLAGGLQGFFIHYMDPRIFGWTTSVEQVIIVYFGGI
                     NSLTGCVVSALLLTTMPELLRFASMWRVTIYAALIILTMNLRPQGLLGDWELTRASAS
                     DLYRRLRRAPVEPQPVEMEVR"
     gene            9228..10004
                     /locus_tag="VHS06_10200"
     CDS             9228..10004
                     /locus_tag="VHS06_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988528.1"
                     /translation="MVAVNGETLLTVDGLCKSFGGVHAVVDFSMELRRDHVVGLIGPN
                     GAGKTTVFNLLSGIIPVDKGRVTFRGTDITNEKSYIIARLGIGRTFQNLRLFDGLSVA
                     DNVKAACCHDVGYSAIAAMLGMPATWSSERALNAKVDGLLELVGIAHCGSERARSLPY
                     GLQRKVEIARALATNPQILFLDEPAAGLNPSEVVEMLSLIRSLHDEQHYSIFLIEHHM
                     EVVMPVCDDIYVVNMGQTIAHGTPEEVQAHPEVLKAYLGE"
     gene            10014..10724
                     /locus_tag="VHS06_10205"
     CDS             10014..10724
                     /locus_tag="VHS06_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988529.1"
                     /translation="MLLEIDNLAVSYGKMAALHGVSLKVAECSITSIIGANGAGKSTL
                     LNAISGVVKYTGRIALDGKPLPRTPHEVVKMGIVQVPEGRRVFSALTVEENLTMGAYT
                     IRNRELIHSRLNQAFELFPILAERRRQLAGTLSGGEQQMLAISRGLMSGPRVLLLDEP
                     SLGLAPRLVKSLFELFTKINEQGVTIVLVEQNAKQALAIAGHAYVLEVGNIVSQGAGV
                     HLLEDPVIRKAYLGASGE"
     gene            10825..11574
                     /locus_tag="VHS06_10210"
     CDS             10825..11574
                     /locus_tag="VHS06_10210"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX2988530.1"
                     /translation="MLLLDRAGCERIHAGALEVLRDVGVRVDDSRIVGLLLDAGATLA
                     GENVVHIPAELVEWAIKTAPKAVRIADRADNLWEIHPGGPVLVLTGNALYITRGKERS
                     NLTSDALAELARVTDACDNIHGMVGTAVVDFPPTCRDFVGFRIMAENCRKHLRPCVYT
                     PRGGRLVLEMAQVLLGGISLKERPIVSSGFSILSPLHWSQLALGVFEETAGFGVPATI
                     NSEPLAGATAPVTLAGCLTIGDAAVLRPGNT"
     gene            complement(11534..>12628)
                     /locus_tag="VHS06_10215"
     CDS             complement(11534..>12628)
                     /locus_tag="VHS06_10215"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX2988531.1"
                     /translation="NALYITRGKERSDLTSDALAELARVTDACDNIHGMVGTAVVDFP
                     PTCRDFVGFRIMAENCRKHLRPCVYTPRGGRLVVEMAQVLLDGISLKERPIVSSGFSI
                     LSPLHWSQLALGVFEETAGFGVPATINSEPLAGATAPVTLAGCLTIGDADVLSGLVIT
                     QLLEPGRPCLYNIGFAHVIDMASAIALTGAPENALLQAAGADMAAFHGLPCASWMSTE
                     SMVADAQAAFEKMITGMAHAAGGVNFMWGAGNLESTLSMSPEALVMDDEIAGYFLRFA
                     QGVPVTDDEMALGLIKEVGAAGDFLTTEHTLLNYKRVLSKAKLAVRDRRALWEQSGSL
                     TFEEAATGRVKKILSAEPKCYLDEAQRHHR"
BASE COUNT         2435 a         3671 c         3999 g         2523 t
ORIGIN      
        1 aactggccga ggggatccgg tctcgagccg agtcggcact ggatggttgg cgggtggccg
       61 cgggagtcgg ccgtccggct cagtcccaca gcggactggt cacgacttac cgtgaggccg
      121 aaggtgcgct gggcatcggg ataagggtga atggcccctc ttccaccacc tatttcggga
      181 gcctgggaat ccttcgtctg cttgcccagg tcgggaacgc cgtggagttg cagtgcttcc
      241 gtgcggagat gttggggaag ctggaggacc atgaccggaa ggcgggcggc gagctgctga
      301 agacattgga ggcgttcttc cagtgccatg gcaatctgag caggacggcg gaggagctgt
      361 cgctccaccg caactccctg ctgtaccgcc tccagcgtgt cgaggagatc agcggactgg
      421 agatggacga tcctgaggtg cggctatcgc tgcaggtagc gctgaaggtg aggcagctgc
      481 tggaagcgga acgagtgggc agaggttagg aatattatcg cgccggggtt gcctcggcat
      541 caccacaaca ggccggattg ggccgtttgt ctattgtaag aggaggcatc agtggcgaag
      601 gaagctggta aggacaggga caagatccag caaatacttg agactgtcga gcgggagcag
      661 gtcaagttcg taaaccttca gctcaccgac atcgtcggaa tggtgaagca ggtcaccatc
      721 cccgctcacg agctgcagga cacgttggag aacgggaagt ggttcgatgg atcctccatc
      781 gagggcttcg cccggatcca cgaaagtgat atgtatctcg aaccggacct ctcgaccttt
      841 ggaatagtac ccttcggcga aggcagcatg acgactgcca agatgacctg caacgtcttc
      901 atgccggatg gggagccgtt cattggagat cctcgcttca ttctgaagag cgccttggac
      961 gaggcccgct ctatggggta cgagttcttc accggccccg agctggagtt ctacctgttc
     1021 cacaccaggg aaaatggtca cccggagccg ttgccccacg acgagggctc ctacttcgac
     1081 atgtccaccg accttgcgtc ggacgtgcgc aaggagatgg tggaggcgct ggagttacag
     1141 gggatcgcgg tagaaaccag ccaccatgag gtcgggcccg ggcagcacga gatcgacatg
     1201 cgctacgacg aggcgttgcg catggccgac aaggttgtgg agttcaagtc cacgctgaag
     1261 tcggttgctc accgccacgg gttgcatgcc accttcatgc cgaagccggt gtttgggatc
     1321 gccggcaacg gcatgcacac ccaccagagc ctctggagca tcgccgaggg acggaacacc
     1381 ttctacgatc cccacgatca gtatggccta tccgatgtcg ccaagcacta catcgccggg
     1441 cagctagccc acgccaaggc gatgtgcgcg gtgctctcgc cgctgatcaa ctcctacaag
     1501 cggttggtgg ccggcttcga ggctccggtc tacgtgagct gggcgcgcac caacaggtcg
     1561 gctctgatcc gcatcccgaa gatcaccggg ggtaagttcg aggccaccag ggtcgagcta
     1621 cggagccccg atcccacctg caacccctac ctggcgttcg ccgtgatgct caaagccggc
     1681 ctggacggta tcaagaggga gttgccgctg gtgcagccga tagaggagga cctcttcgag
     1741 ttcacggacg aaatgatggt tcgccacggt gtgggtgtcc tgcccggctc cctccaccag
     1801 gcgctggacg agatggagaa ggacgaggtg gtaaaggagg ctcttggccc ccatgcctac
     1861 gcgcgcttcc tcgaggccaa gacgattgag tggaagagct tcagacagac ggtgacttcc
     1921 tgggagcttg agcgctacct ggggatctac taatcctgag acacggagac cctcacctat
     1981 aggagcccgg ggcatccccg atttcgacgg cgccaggatg ggccgcccgg gtcagggacg
     2041 acccgggctc ctagtgtcgt ggccggcgtg tccgaaagcc atgaccagag acgggcgggt
     2101 caggggcgac ccgccctacg atgtctctga gatcaacaaa cagggggccg catacgcggc
     2161 cccctgtttc aatgttcctg cctctagccg actttgaccg gctgttcggt ctgagcctgg
     2221 aactccatgg ctcggtgata cagttcgacg tacttgcggg cggtggattc ccaggagtag
     2281 tctgccttca tcccggtctc catcaaccgg cgccagaagt tgtggtgctt gtaactctcc
     2341 atcgccctca cgatggcggc gaagagcatc atgccgctgt acttgtcgaa ggtgaagcct
     2401 gttccctccc ccgtgcgcgg gtcgaagtcc ttgacggtgt ctgccagccc gccggttcgg
     2461 cggaccaccg gcacgctgcc gtagcgcatc gccaccatct ggttggagcc gcacggctcg
     2521 aagcgtgacg gcatgaggaa gatgtcgctg ccggcataga tcttttgcgc aatagccgag
     2581 ttgaaggtga ggaacaccgc gaccttcccc gggtacctgc gcgcaacgcg ggtgaacatc
     2641 tcgtggtagt actggtcgcc cgtacccagg atggccagtt ggaggggcag gtccataaga
     2701 ccgtcgatca cctctcccag gatgtccaaa cccttctggc tcgtcagtcg ggaaatacag
     2761 ccgatcaggg gcactttctc gtctacagca aggttggcct ctttctggaa ggccagcttg
     2821 ttgactacct tcttatccag ggaggcgatg tcgtaatgct gggccaacgc cgggtcggtg
     2881 gccgggttca tcgcctcgta atccactccg tttagtatgc cgaacagccg atccttccga
     2941 tcgttcagca gggtgtgcag cttctcccca tactcggcag tgaggatctc cttggcgtag
     3001 ctctcgctga cggtgctgat cacgtctgcg aagaggatgc cgtgtgccat tagtgcgacc
     3061 acgttgggga attcctcgat ctgcgggtac atgaagctgt attcgtccac accggcgatt
     3121 tccagtatgc gccagccgaa gatcccctga taggccaggt tgtggatggt gtagaccgac
     3181 gcgacgtttc ggaagaaggg gtcttccttg tagatggtct tcagccagtt ggccacgatg
     3241 gcggtgtgcc agtcgttcag gtggatgacg tcgggcttcc agtccagcag cttcagcatc
     3301 tccatgactg cccggcaata gacgatgaag cgctcgccgt catccgggta gccgtagagg
     3361 ttctccctgg cgaagtactt ctcgttgtcg atgaagtaga cggggaggtt gccctcgagt
     3421 tcggtcgaga gaacgtttat cgcttcctgg tggtcgtcca ccgggatttg cacgccggaa
     3481 aggactgggc ggagcgagaa ccttgcaggg tcgatgcggc catgccttgg aatagcgacc
     3541 cggacgtcgt ggcccagatt gcggagagcc ctgggaaggg ccgcggcaac ttccgagacg
     3601 cctcccgtct tggcaaaggg ggccatctct gcggaaagga agaggatttt gagcggatca
     3661 gccatcgatt cgaaccctcc caccacactc gctgcccacg ggaccaggag ttgggacgtt
     3721 caacgctaca taaagagtgt agcaagaaat ggtccggaag tcgcgggctt cccgggcgga
     3781 agggacccaa gcgtatccat gccgcccctc tgtctggtct ggggtgcccg atgttagcac
     3841 aaaagaggag aggcaacaac gatggacata acctcgaacc atcatccact tgacaccgtg
     3901 ccgtgggggg agaataatga gcgcacctcg caacatgtaa gaagcaagat atatcttgca
     3961 aagaactgac caccgtcctg gcagatgggg aggagcgagt tggaccagtt ggacccccac
     4021 tacgaaatcg accgcaggga tctctcggat cgcgtctacc aggtcctgcg cacgcagatc
     4081 ctttccggca agctcgcccc aggccaacgg ctctccctcg acgagttcgc tcagtacttc
     4141 aaggtcagca ttacccccgt tcgcgatgcg ctcagacagc tctcctccga cgggttggtc
     4201 gagcttcagc ctcgccgtgg tgccttcgtc acgcagccca cctggtctca cgtcgaggag
     4261 gtgtaccaga tccgcgagat cctcgagtgc gcggctgtcg agttcgttat ccggaggggc
     4321 ggcgaggctc ttcgggagat ggagcaactt gtcgaggaga tcatcggcac caccgtcggc
     4381 gaggtccact cagactacct ggcctacatc cgactggatc agcgcttcca ccagttcctg
     4441 atcgattgcg ttggcaacaa gaagctctca gagatctatg ccggcctgcg cagccacacc
     4501 ctggtcaccc ttgcccttta cagttcggct gatcaacgtg ccaccgatac cctatctgag
     4561 catgaggcca tcctcgatgc gcttcgcagg ggcgatgtgg tcgccgcgca gtcggcccta
     4621 cgcactcacc tgcgcaacgc caaagccgag atcgagcgga aggttgtcga aggcaagctt
     4681 ccggtcaatg ggacgcactc cactccgctt ctgtaggtcg tagcgtcatt cggcggctat
     4741 cggacgcgtg ggcgaagatc gccattcaac atcgagtccc tggggcatac tctcgtttcg
     4801 acgtgagggt cgcggtccaa cggcagactg ggctccacta cggcgctagg ggttgatcga
     4861 tgtcaaagac ggttcacgtg ggagtggtcg gggcgggtat tgccgccgag ttccatcttg
     4921 ccgcctaccg gcgggtgtca ggctactcgg tcgagatcgc tggaatcacc tctcgtacgc
     4981 catcgaaagc cgcggcgctg gccgagcagt tcgggatcca gcgcctctat ccggatctct
     5041 cctctcttct ctccgacagg gagatcgacg tggtggacct ctgcgttccc gtgcacctgc
     5101 actgctcgat ggcgatccaa gccgccaagg ccggcaagga cgtcgtgtgc gaaaagccgc
     5161 tactcggttt tgccgggaat ggacgaaccg gtagtgttcc ccggggcgag atgttctccg
     5221 ccgttcagga tgagttggaa gcggtggaag ctgccttccg tgcctccgga acaaagctac
     5281 tatacgccga gaactgggta tatgccccgg ccttccggcg gattgcgcag atggcagagt
     5341 cctgtggtgg caagatcctg gagattcgtg ggaatgaggc ccatagcggg tccgccagcc
     5401 ctttctccat gaaatgggag acgtctggcg gcgggtcgtt gctgcggctc ggcatccacc
     5461 ccctgagcgc ggccatctat ctgaagcagc gtgaagggat cgtgaagcag ggttacccct
     5521 atcgggtcag atcggcttgg gcgcagatgt cggacctgac ccgcgtcgac ggtttcgatc
     5581 cggccacgac caggttagta tctggctggg tcgacgtcga gaactgggca gttggcgtac
     5641 tgacgttcga cgataactct gtggcggtga tccacagttc cgacatctcg ctcggcggcg
     5701 taagcagttg gatggagctg tacacgacca acgctcggct gcgctgcaac atccacccca
     5761 acgatacctg catctcctac tcgcccgatc cccggccctt cgacggtctg ttcctgaacg
     5821 agaagctgga gacgaaagcg ggttggaact atcccagcgt cgatcacaac tgggaggcgg
     5881 gctacgtcca ggagatcgag gacttcgtgg catcggtcgc cgaggatagg gagccccttt
     5941 ccggcttgga attggcgttg gagtcgatcc gagcagcata cgcgctgtat atgtcggccg
     6001 aggagggggt gcgggtcgat ctgtagttct ttgagccgtg gctgtctagc gttctctttg
     6061 tggagagtcg tggaggagag gagaaggaat cggaggcgtg gtccccggaa gtccatctgc
     6121 ggagtcaatg ggtaggcaat tgggttttcc cctggggctg taacttgaag acctagatcg
     6181 agagtcggga aaggagatcc gatcgtgaga cgcttcagtt ggctcgttgc cgtcttcgcc
     6241 gtcctgggga tgtctctggg ggtggttctg ttgtccgggt gtgattccgg tggccccagc
     6301 gaaatcaagg tgggcatcat agaggacctg agtggagcga cctcggcctg gggtgagggg
     6361 cttcagaacg gtgccatcta tgccatcgag aagatcaaca aagagggcgg gctgctcggg
     6421 aagcagatca agtacaccag ctacgacttc aagggaaacg tgccggagag tctgactgcc
     6481 tacaatcggc tggtcgacca ggacaaagtc gtcgcggtga tcggcactcc caacagcgct
     6541 atgcacatcg cccttgcacc gttggccgac gaaaagaagg tgccggtgat cggcgatccg
     6601 atggacgagc gagctacaac ccccgaagcc aacaaggtaa acgcctacac cttcctcggt
     6661 gagcccgccg caggtgaaca ggccagagcc atcgccagct acaccctaaa cgagatgaag
     6721 ctgaagaagg tggccattct cttcgatcag ggcaatcctt actccgtgtc gctggctact
     6781 cccttcgccg aatacttcag caagaatggc ggccaggtcg tagctaacga gccgttccag
     6841 tccggtacca acgacttccg agctcaactg accaagatca agcaatccag tcctgaagtt
     6901 ctgcacataa ccagctatct caaggagaac tcgctgatcg tcgcccagat gagagagttg
     6961 gggatgaata tcccggtagt aggcaacaac tcctgcttct accccttcgc cgagaccgct
     7021 gggaaggatg ccgagggggt tgtcttccca aacaacatct cactggacga cccgcgctac
     7081 caggactata tcagcggcta caaggctcga ttcggcaagg atcccgtggt tcatgcctgg
     7141 atgggttacg acaacatgat gatcctggcc aatgccatca agaaggctaa caagatcgac
     7201 tcggtcgcaa ttcgagatgc catcgagaac cagaccaaag acgttcaggg catgaccgac
     7261 aagatcacca tcgacccgaa gacccaccgg ccggtgaacc tgccgctggc aatccttcag
     7321 gtcaaggacg ggaagtacgc gaccctgaag gcgagctacc tgcccaagta gttgtctcgg
     7381 aggatcgacc gagggatcag ccttcggtcg atcctcccgt ccgaactcag cgaggcaagg
     7441 tgagcgatgt acgtacttca gcagttgatc aatgccctct ccgtgggggg cgtctacgcc
     7501 ctggttgccg tgggttttgc cctcgtatac agcattctca agttcagcaa ttggtcccac
     7561 ggtgcggtga tcatgctgag ctcctacttc gggtatgtca tggcatcccg cgttggcttg
     7621 ccattggtcc cgacgttgct gctcaccatg gttgctggtg gcttcctggc ggtgatcatc
     7681 gagcttgtcg catttcgtca gatccggttg aagaatgggc cgctgatcta cttcttcgta
     7741 acctccatca cggtttccac aggggcgcag tttgccatgt acccgaccgt tgggggcaat
     7801 ttcttccgat ggccggagat catctcatcc accgcaatcc agatgggagg ggtgaccgta
     7861 tctaccctga atctgatgat gggtggtgtt tgcctgctgg cgttggttgt attgaacctg
     7921 atcctgtttc gcacgcgtat cggcacggca attcgggccg cctctaacga tgtgcgggcg
     7981 gccgcactga tgggagtgga catcaacttt gtgatcgcgg caacgttctt cctctccggg
     8041 gcgttgggag ggttggcggg tacgctgatg ggcatcagct acaccaccta tcccgagatg
     8101 ggtgctgagg cgatggtcaa agggtttgtc gccgcggtgg tgggcggcct tgggagtatc
     8161 agcggggctg tgatgggggg catcttgctg gctctggtgg agaccgtcgt cacgatgacc
     8221 ccagtcggtt cgtcggccgc gccggtggcg gttttcgcca ttctgctggt gttgctgttc
     8281 gtcagaccct ggggcattgc cggcaaagcc accgacgaga aggcgtagac tgagatgcag
     8341 tactatgttg atctggccac actgtttctg atcgcgctga ccgcggttgt gggggtcttc
     8401 gccctcaccg ggctcacggg gctcttctcg ttcgggcagg ggggcttcat ggccctgggg
     8461 gcctatacgg cagtcctgac caacatgcga ctgggcgttc ccttccccat cgctctactg
     8521 ctgggggttg tgatggcggt tgccgccagt ctgttaatcg gctttcctgc gctgaagctg
     8581 cggcgagact tcttcgccct ggcaaccttc ggctttgggg aggcggtgac ggccatcttc
     8641 cagatgttta cgcaggtgac gggcggcaca atggggctgc ccagcatacc gcgctacacg
     8701 gagttttgga tggcgctgct cgttgccgca agtgcggtct acctggttgc caatttcaag
     8761 aatacgaaga tgggacggca gtccattgcc atcagaaacg atgaactggc cgcacaggcg
     8821 ttggggatca acgccttccg ccacaagatg atcgtcttcg ccttcagcgc tggactggcc
     8881 ggcctcgccg gagggctaca gggtttcttc attcactaca tggacccgag gatctttggt
     8941 tggaccactt cggtggagca ggtgatcatc gtgtacttcg ggggcatcaa cagtctcacc
     9001 ggttgcgtgg ttagtgcgct gctgctgacg acgatgccgg agttgttgcg ctttgccagc
     9061 atgtggcgcg tgacaatcta tgctgccctc atcatcctga ccatgaacct gcggcctcag
     9121 gggcttctgg gcgactggga gctcactcgc gcttcggcta gcgatctcta ccgtcggcta
     9181 cggagggccc ccgtggagcc ccagccagtc gagatggagg tgcgctgatg gtcgcagtaa
     9241 atggcgaaac gctgctgacc gttgatggcc tctgcaagag cttcggtggg gtccatgccg
     9301 tggtcgattt cagcatggaa ctgaggagag accacgtagt ggggctgatc gggcctaacg
     9361 gagctggaaa gactaccgtc ttcaacctcc tttccggcat catccccgtc gacaagggga
     9421 gggtgacctt ccgaggtacc gacataacga atgagaagtc ctacatcatt gccagactag
     9481 gcatcggccg aaccttccag aacttgcggc tgttcgatgg cctgagcgtc gcggacaacg
     9541 tgaaggcggc ctgttgccac gatgtcgggt acagtgccat tgccgcgatg ctggggatgc
     9601 ccgcgacgtg gagtagtgag cgcgcgttga acgccaaggt ggatggtttg ctggaactgg
     9661 tggggatcgc tcactgcggc agcgagcgtg ccaggagcct tccctacggc ctgcagcgca
     9721 aggtggagat cgccagggcc ctggccacaa acccgcagat ccttttcctg gatgagcctg
     9781 ccgctggcct gaaccccagc gaggtggtgg agatgctctc tctgattcgc agtctgcacg
     9841 acgaacagca ctactctatc ttcctcatcg agcaccacat ggaggtggtg atgccggtgt
     9901 gcgatgacat ctacgtggtg aacatggggc agaccattgc ccatgggacg ccggaagagg
     9961 tgcaggcgca ccccgaagtg ctgaaagcct acctgggaga gtgagagcca accatgcttc
    10021 ttgagataga caacctggct gtttcttacg ggaagatggc agcgctccat ggggtttccc
    10081 tgaaggtcgc ggagtgcagc atcacctcga tcattggggc caacggtgcg ggcaagagca
    10141 cgctgctcaa cgccatatcc ggcgtcgtca agtacacagg caggattgcc ctggatggga
    10201 aaccgctgcc aaggaccccc cacgaggtgg tcaagatggg catcgtccag gtgccggagg
    10261 gccggagggt gttttctgcc ctgacggtgg aagagaacct gacgatgggt gcctacacca
    10321 ttaggaaccg ggagttgatc cattccaggc tgaaccaggc attcgaactg tttccgatcc
    10381 tcgccgagag acgccggcaa ttggcaggca ccctctccgg cggtgagcag cagatgctgg
    10441 ccatcagccg tggcctgatg tcggggccga gggtgctgct gctagatgag ccctcccttg
    10501 gactggcgcc caggctggtg aaatcgctct tcgaactgtt cacgaagatc aacgagcagg
    10561 gggtgaccat agtgctggtg gaacagaacg ccaagcaggc gttggccatc gctggacacg
    10621 cctacgtttt ggaagtgggg aacatagtct ctcagggtgc cggtgtccat ttgctggagg
    10681 acccggtgat ccgcaaggcg tatctgggtg ccagcggcga gtagcctctg ccagcgtagg
    10741 ggtgacgcat gcgtcgcccc tacagactgt cagtgtagtg gccggcgacg gccgccaagt
    10801 cgtgctcttg gggggagtcc gaagatgctg cttctggacc gtgcagggtg tgagagaatt
    10861 cacgccggtg ccctggaagt tttgagggac gttggcgtgc gggtggacga tagccgaatc
    10921 gtcgggctgc tactggatgc tggtgccacg cttgccgggg agaacgtggt ccacatcccg
    10981 gctgagctag tggagtgggc aatcaagacc gctcccaagg cggtgcggat tgccgatcgg
    11041 gcggacaacc tgtgggagat tcatccgggt ggaccggtgc tggtgctgac cggcaacgcg
    11101 ctctacataa ccaggggcaa ggagaggtcg aaccttacct cagacgccct ggctgaactt
    11161 gctcgggtga ccgacgcctg cgacaacatc cacggcatgg tgggcacggc ggttgtggac
    11221 tttccgccga cgtgccgcga cttcgtcggc ttcaggatca tggccgagaa ctgccggaag
    11281 cacctgcgcc cctgcgtcta cacccccagg ggcggcaggc tggtgctgga gatggcccag
    11341 gtgctgctgg gcggcatctc cctcaaagag cggccgatcg tcagcagcgg tttcagcatc
    11401 ctcagccccc tgcactggag ccagttggcg ttgggcgtgt tcgaggagac cgccggcttc
    11461 ggcgtgcccg ctaccatcaa ctccgagccg ctggccggcg ctaccgctcc tgtcaccctt
    11521 gccggctgcc tgactatcgg tgatgccgct gtgcttcgtc caggtaacac ttaggctcgg
    11581 cgctcaggat cttcttcacc cgccccgtcg cggcctcttc gaaggtcagc gatccgctct
    11641 gctcccacag agcccgccga tcgcgtacag ccagctttgc cttgctcaac acccgcttgt
    11701 agttcagtag ggtgtgctcg gtagtgagga aatcccccgc ggcacccacc tccttgatca
    11761 accccaacgc catctcatcg tcggtgactg gcaccccctg ggcaaagcgg aggaaatacc
    11821 cggcaatttc gtcgtccata accagcgcct cgggtgacat ggagagggtg gactccaggt
    11881 tcccggcgcc ccacatgaag ttcacgccac cggctgcgtg ggccatgcct gtgatcatct
    11941 tctcgaaggc ggcctgagca tcggcaacca tggactcggt tgacatccag gaagcgcagg
    12001 gaagtccatg gaaggccgcc atgtcggctc ccgcggcctg cagcagcgcg ttctccggcg
    12061 cgccggtcaa cgctatggcc gaggccatat cgatcacgtg ggcaaatccg atgttgtaaa
    12121 gacaggggcg ccccggctcc aacagttgag tgatcaccag cccactcagc acatcggcat
    12181 caccgatagt caggcagccg gccagtgtaa caggagcagt agcgccggcc agcggctcgg
    12241 agttgatggt agcgggcacg ccgaagcctg cggtctcctc gaacacgccc aacgccaact
    12301 ggctccagtg cagagggctg aggatgctga aaccgctgct gacgatcggc cgctccttga
    12361 gtgagatgcc gtccaacagc acctgggcca tctccaccac tagcctccca cccctgggag
    12421 tgtagacaca ggggcgcaag tgctttcggc agttctcggc catgatcctg aagccgacga
    12481 aatcgcggca cgtcggcgga aagtccacaa ccgccgtgcc caccatgccg tggatgttgt
    12541 cacaggcgtc ggtcacccga gcaagttcag ccagggcgtc tgaggtaagg tccgacctct
    12601 ccttgccccg ggttatgtag agcgcgtt
//
LOCUS       DATALS010000459         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_441517, whole genome shotgun sequence.
ACCESSION   DATALS010000459 DATALS010000000
VERSION     DATALS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441517"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1073)
                     /locus_tag="VHS06_10220"
     CDS             complement(177..1073)
                     /locus_tag="VHS06_10220"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX2988532.1"
                     /translation="MDNKVVGEMDERRITGLTRLLLAGGVAGPILFIIVFLIEGATRP
                     GYSPWRNFVSQLSTGEGGWVQIANFIMCGVLSLGFALALWSSSSPRLARRLAWGPALL
                     ALFSLALLAGGAFVTDPALGYPPGTPLGSQGQTWHGAVHGVAGLLAFGLLAAACFVYA
                     RRFSGDPEWRGWTAYSSITGTIIALGFIASTVTSVLDERGVVPNAPTGVIQRVAIIVG
                     WGWIAIFALRLLRDPRLLVLLGQVNVSTRGDGTSGFKSRVVAIAVTIIVLIVVILVVL
                     WLAGVFRGGGAATHGAIPQLPI"
     gene            1514..2869
                     /locus_tag="VHS06_10225"
     CDS             1514..2869
                     /locus_tag="VHS06_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988533.1"
                     /translation="MKGKVIRWLPAVLAALFAIGMLPITPSSAQETSRTFPETGKSVS
                     GKFLQYWDTHGGLAQQGIPISNEMQEKSDTDGKVYTVQYFERAVFELHPENQPPNDVL
                     LQLLGVFRYKEMYPGGAPGQVASSEPGAVLFNETGHRVGGLFLAYWQSHGGLAQQGFP
                     ISEEFNEVSALNGQTYRVQYFERAVFEYHPENQPPHNVLLSQLGKFRWDEKNDPTPTP
                     TLPPPPPRPVSPTPTPVPHDPNTDCSGIPASVNQEVRPNCGPAGTKFTFRGWGFKPGE
                     KVGVYVTEPSQAVYGPPWQATASSSGEVRVSLTTGTRDPAGIWATTMEGIETHAKAIG
                     YFKVTGSGTPPPPTGCSDVPAPVSMKVAPSTCAQGGTRFEFLGSGFTPGEKVGVYVTE
                     PSQAVYGPPWQIGVDGEGYTDVVSFQTEPDFPTGIWAMTMEGVTSHHKAIGYFKILPP
                     K"
     gene            complement(2963..3973)
                     /locus_tag="VHS06_10230"
     CDS             complement(2963..3973)
                     /locus_tag="VHS06_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX2988534.1"
                     /translation="MEPAPSLELPALTIISLGGTIAMTQAGGGGVTPTLTGEDLVAAV
                     PALAGVANVTALSFRQLPGAHLTMDDLVSLAAEIERRIEAGAAGIVVIQGTDTIEESA
                     FALDLLLEGAAPVVVTGAMRNSTLPGADGPANILAAAQVAADRAARDLGVLVVLNDEI
                     HAARYVRKIHPTSPSAFASPIAGSLGRVVEGRPRIFARLPGRRHIDAAAFASDRRVAL
                     LTVALGDDGRLLEAVREAGYGGLVVEAFGAGHVPATLVEGLGRLAAAMPVVLASRTGG
                     GPMMEHTYGFPGSESDMLGRGLISAGILDGPKARILLWLLLGTGASSAEISREFGWWA
                     AV"
BASE COUNT          807 a         1433 c         1262 g          690 t
ORIGIN      
        1 gtcatcctcc tgctggcgtg cctggcgtgg ctcttcatcc gcgagatacc tctccgctcc
       61 agccaccacc ccgagaccga agaagtcgca gaggcgggtc gcctctagat ctcatagagc
      121 ttgagaaggc ctccccgtcg cggggaggcc ttctatgtgt atcaagtcca tttaccctag
      181 atagggagct gaggaatcgc gccatgagta gcagcgccgc cgcccctgaa tacgcccgcg
      241 agccacagca cgaccagtat caccacgatc agcacgatga ttgtcacggc gatggccacc
      301 accctggact tgaagccgga cgtgccgtcg ccacgcgtgc tcacattgac ttgacccagc
      361 agcaccaaca ggcggggatc gcgcagtaac cgcagggcga agattgctat ccatccccag
      421 cccacgataa tcgcgacgcg ctggatcacg cccgtggggg cgtttgggac gacgccgcgc
      481 tcgtccagaa ccgaggtcac ggttgaggcg atgaaaccca gcgctattat cgtccccgta
      541 atgcttgagt aagcggtcca gcctctccat tcggggtcgc cgctgaaacg ccgagcatac
      601 acgaagcagg cagctgccag caggccgaag gcgagcagcc ccgcgacccc atgaaccgcc
      661 ccgtgccagg tctgcccttg cgagcccaga ggtgtgccgg gtgggtagcc cagagccggg
      721 tcggtcacga aggctccccc cgccagcaga gctaagctga atagtgccaa caacgccggc
      781 ccccaggcca agcgtctcgc gagacgaggt gaggacgatg accacagcgc cagcgcgaaa
      841 ccgagactga gcaccccgca catgatgaaa ttggcgattt gcacccagcc gccttcgccc
      901 gtgctcagct ggcttacgaa atttcgccag gggctatagc ctgggcgcgt ggccccttct
      961 atcaagaaga cgattatgaa cagtatgggg cccgccacgc ccccggccag caggagccta
     1021 gtgagcccgg tgatacgtcg ctcatccatt tcacccacca ccttgttgtc cactctatcc
     1081 tctcctgttc aagtagggaa gggcgcagga gcctccccag gtctctgcat taaacactat
     1141 gttgggcgaa agtgcactag gtagttttgc ctatggcaga gcccggcctc ctcttcgccg
     1201 ggttaggcaa tcacgccgac gcggagatcc gcggcagcgg atacgataac gatgaagcaa
     1261 ggccggcccg ggctcggggc cgttttccgg gccgacctgc cggtcgatcc gcccgccggc
     1321 tccgtcctcc ccgcgcacca ccacatcgtc atcctcatac gttcccccgc aggcgccgga
     1381 gccgaaaagg cgaaacggga gttctcatga caacacgcac tttagtgcga tgtcgaaggc
     1441 aaagccggtc ggggtgacgc tcggtgaggg cccgtccgcc ctccgtaccg gcaagtatag
     1501 gaggaggaac gagatgaaag gcaaggtaat tcgctggctg cccgcggtgt tggcagcgct
     1561 gttcgctatc ggtatgctcc ccatcacgcc ctcgagcgcg caggagacaa gccgcacttt
     1621 cccggaaacg ggcaagagcg tgagcgggaa gttccttcaa tactgggata cccatggcgg
     1681 cctggcccag cagggcattc ctatctcgaa cgagatgcag gagaagagcg ataccgacgg
     1741 caaggtctac accgtgcagt acttcgagcg cgcggtcttc gagcttcacc ccgagaacca
     1801 gcctccgaat gacgtgctgc tccaacttct gggagtcttc cgttataagg agatgtaccc
     1861 gggcggcgcg cccggccagg tcgcaagtag cgagcccgga gccgtgctgt tcaacgagac
     1921 cggccataga gttggcggcc tcttcctggc ctactggcag tcgcacggtg ggctcgccca
     1981 acagggcttt ccgatatctg aagagttcaa cgaagtatcc gccctcaacg gccagaccta
     2041 cagggtgcag tacttcgagc gggcggtatt cgaatatcat cctgaaaacc agcccccgca
     2101 caatgttctt ctctcgcagc taggcaagtt ccgctgggac gagaagaatg atcccacacc
     2161 cacacctaca ctgcccccgc ctccacctcg gcccgtgagc cccaccccta cgcccgttcc
     2221 ccacgacccc aacactgact gctcgggcat cccggcgagc gtgaaccagg aggtccgccc
     2281 caactgcggc cccgcgggca ccaagttcac gttcagaggc tggggattca agcccggtga
     2341 gaaggttggc gtgtatgtta ccgaacccag ccaggcggtt tatggccctc cgtggcaggc
     2401 gacggcaagc agcagcggcg aggtgagagt gtccctgacc accggcaccc gagacccggc
     2461 ggggatctgg gcgaccacga tggaggggat tgagactcat gccaaggcga tcggctactt
     2521 caaggtgacc ggctccggca ctcctcctcc acccacgggg tgcagcgatg tgcccgctcc
     2581 cgttagcatg aaggtggccc cgagcacctg cgcccagggc gggacacgat tcgaattcct
     2641 tggctcgggt ttcacgccgg gtgagaaagt tggcgtgtat gtcaccgagc ccagccaggc
     2701 agtgtacggc cctccgtggc aaattggcgt agacggcgaa ggctacaccg acgttgtctc
     2761 cttccagacg gagcctgact tcccgactgg gatctgggcg atgactatgg aaggcgtaac
     2821 ctcgcaccat aaggccatcg gttacttcaa gatcctgccg ccgaagtagc ccgtgcccgc
     2881 gccatcgccc cgccgcgatg gcacgacaag gagagggagg gcgctgtcgc cctccctctt
     2941 aagcatcccg acgcgcccca ctttataccg cggcccacca gccgaactcg cgagagatct
     3001 cggcgctgct cgctccggta cccaggagca gccacaggag tatcctcgct tttggcccgt
     3061 cgagtatccc cgcgctgatc aggcctcgtc cgagcatatc gctctccgag ccggggaatc
     3121 cataagtgtg ctccatcatc ggtccgccgc ccgttcggga ggcgagcacc accggcatgg
     3181 cggccgccag tcgccccagt ccctcgacga gcgtggcggg tacgtggccc gctccaaatg
     3241 cctccaccac caatccgccg tatcccgcct cgcgcacggc ctccaggagc cgcccgtcgt
     3301 caccgagcgc gaccgtcagc agggcgacgc gccggtcgct cgcgaaggcc gcagcatcaa
     3361 tatggcgacg gccgggcagc cgtgcgaaga tcctcggccg cccctcgacc acccggccga
     3421 gcgaacccgc tatgggcgaa gcgaacgccg aggggctcgt cgggtgtatc ttcctgacat
     3481 agcgtgccgc gtggatctcg tcgttgagca cgaccagaac cccgagatct ctcgccgccc
     3541 ggtccgccgc tacctgcgcc gcggcgagta tgttggcggg cccatcggcg ccggggagag
     3601 tggagttgcg catcgcgcct gtcaccacca ccggcgcggc gccttcgagg agaaggtcga
     3661 gcgcgaaagc cgactcctcg atcgtatcgg tgccctggat aaccacgatg ccggcggccc
     3721 ccgcctcgat cctgcgctcg atctccgccg ccaacgacac aaggtcgtcc atcgtgagat
     3781 gagctcccgg cagctggcgg aaggacagag ccgtgacgtt ggccaccccg gccagcgccg
     3841 gcaccgccgc gaccagatcc tcgcccgtga gtgtcggtgt tacgccgccc ccgcccgctt
     3901 gagtcatggc gatggtgccg cccagcgaga tgattgtgag ggccggaagc tccaggctcg
     3961 gtgccggttc catatcccct ccagcgatcc ttggtatcac atatcgagac atagaaggct
     4021 tggcttctcg tcccctcgca ctatagcaca acgatcacta cgtggtgggc tcgtgggtgg
     4081 ttcttggagt gcgatggatg tgtcgcgaca tatcccgtaa gcgcaaacca acaagagaac
     4141 acccgaaccc ccgggaccgg ccgcccccgc cgtggtatac tgggccaccg tc
//
LOCUS       DATALS010000460         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_441890, whole genome shotgun sequence.
ACCESSION   DATALS010000460 DATALS010000000
VERSION     DATALS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441890"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VHS06_10235"
     CDS             complement(<1..208)
                     /locus_tag="VHS06_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988535.1"
                     /translation="MGPVGVAVGEGVGVAVGVRVGAGVDVAEGIGMAVGLSADMGDGE
                     DAATMLASPCPVCVMIPMQNSNTED"
     gene            120..1040
                     /locus_tag="VHS06_10240"
     CDS             120..1040
                     /locus_tag="VHS06_10240"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX2988536.1"
                     /translation="MPSATSTPAPTRTPTATPTPSPTATPTGPIYPLAVMVENMIDAR
                     PQSGLAKADVVYEALVEGGITRFMAVYKDGKASEIGPVRSARHYYVYLAAEFNAAYVH
                     IGASPQGYVALDASRVTNLDETYGDPGFWRSDARYAPHNAYTSTDLLRQSLDKVRELS
                     PGSMAGFKFGEQPKTAQGTPSYGLTIYYPGGYEVGYGYSPETKSYLRSMDGLPHRDAD
                     SGERVSPLNVVVQVIRSWTIDDIGRLDMEQVGQGKALFFRDGVVSEGSWHKRSLTAPT
                     QWLDANDTPVLMKPGKIWVQMIPPGTKMEY"
     gene            1129..1824
                     /locus_tag="VHS06_10245"
     CDS             1129..1824
                     /locus_tag="VHS06_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2988537.1"
                     /translation="MAGETILVVEDEPKIVELLKLYLERDGFKVEVALDGAVALQMFR
                     RNAPALTVLDINLPTVDGLEVCRQIRRESSLPIIMLTARDEEADKLIGLELGADDYVT
                     KPFSPREVVARVRAVLRRASAPPLSQELLRAGELTLDAARHLVRRGERLLELTPTEFR
                     LLEVLMRHPGRVYSRLQLLDQVQGEAFEGYERTVDAHVKNLRQKVEPDPQHPKLIQTV
                     YGVGYRFAEDPRA"
     gene            1817..>2101
                     /locus_tag="VHS06_10250"
     CDS             1817..>2101
                     /locus_tag="VHS06_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988538.1"
                     /translation="MRSLSFKLAAGFVAVALVGVLIVGYLANRSTTAEFGAYLEGNGP
                     SLEQRAADYIAGRFSSSSGWQGISSTLNPLSRWLGERLILVDGDGKIVADT"
BASE COUNT          425 a          663 c          642 g          371 t
ORIGIN      
        1 cgtcctcagt attgctgttc tgcatcggta tcatcacgca tacggggcag ggcgaagcca
       61 gcatcgtagc cgcatcctcg ccatcgccca tatcggcact caatccaacc gccatcccaa
      121 tgccctccgc cacatccacg ccggcaccaa ctcgaacccc gacagcgaca ccgacaccct
      181 cacccacggc cacaccgacg ggtcccatct atcccctggc cgtgatggtg gagaacatga
      241 tcgatgctcg gcctcaatcg ggactggcca aggcagatgt ggtgtacgag gcgctggtgg
      301 aagggggcat cactcgcttc atggcggttt acaaggacgg aaaggcctcg gaaatcggtc
      361 cagtgcgcag cgcccgacat tactacgtgt atctggccgc cgagttcaac gctgcctacg
      421 tccacatcgg cgccagcccc cagggttacg tcgccctgga tgcgagccgc gtcaccaacc
      481 tggacgaaac ctacggggac cccggcttct ggcggtccga cgccaggtat gcccctcaca
      541 acgcctacac cagcaccgac ctgcttcggc aatcgctgga caaggtgcgc gaactgtcgc
      601 cgggctccat ggcggggttc aagttcggag agcagcccaa gacagctcaa ggcacgccat
      661 cctatgggct caccatttac taccccggcg gctacgaggt cggctacggc tactccccgg
      721 agaccaaatc ctacctgcgc tccatggatg ggcttccgca tagggacgcg gatagcggag
      781 agagagtctc ccctctcaac gtcgtcgtgc aggtcatcag gtcctggacg atcgacgaca
      841 tcggcaggct ggacatggag caggtcggcc aggggaaggc cctcttcttc cgtgatggcg
      901 tggtatccga gggaagctgg cacaagcggt ccctgaccgc gcccacacag tggttggacg
      961 ccaacgatac gccggtgctg atgaaacccg gcaagatctg ggtgcagatg atcccgccgg
     1021 ggaccaagat ggagtattag cccatcccct ccttcacagc ttcttcacta cctcctgcta
     1081 taatcggttc agtagggcaa acagatgctt gcgacctgga gtggaacagt ggccggagag
     1141 acgatcctcg tggtggaaga cgagcccaaa atcgtggaac tgctcaaact ctaccttgaa
     1201 agggatggtt tcaaggtaga ggtcgccctg gatggcgcgg tggcgctgca gatgtttcgg
     1261 cggaacgcgc ccgcactgac cgttctggat atcaacctgc ccaccgtgga cggcctggag
     1321 gtgtgccggc agatccggcg agagtcctcc ctccccatca tcatgctcac ggctcgcgac
     1381 gaggaagcag acaagctgat cggcctggag ttgggtgccg acgactacgt caccaaacca
     1441 ttcagtccta gagaagtagt tgcgagagtg cgggcagtgc tccgtcgagc atccgctccg
     1501 ccgctgtctc aggaactgct ccgggccggc gagctcacgc tggacgccgc acgccatctg
     1561 gttcgccgcg gcgagcggct gctggagctg acgcccaccg agtttcgcct tctggaggtg
     1621 ctgatgcgac acccgggcag ggtctacagt cgtctccaac tgctggacca ggtgcaaggc
     1681 gaggcattcg agggctacga gcgaacggtg gacgcccacg tgaagaacct tcggcaaaag
     1741 gtagagccgg acccacagca tccaaagctc attcagacgg tgtacggcgt cggctacagg
     1801 tttgcggagg atcctcgtgc gtagtctatc cttcaagctg gcggccggct tcgtggccgt
     1861 ggcgctggtg ggagtgctga tcgtcggata cctggcgaac cgatcaacca ccgcggagtt
     1921 cggcgcctac ctggagggca acggccccag tctcgagcag cgcgctgcag attacatcgc
     1981 cggccgattc agcagcagtt cgggatggca gggaatatca tctaccttga atccactgtc
     2041 cagatggctg ggcgagcggc tgatcctggt ggacggtgac ggaaaaatcg ttgcggacac
     2101 c
//
LOCUS       DATALS010000461         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_441960, whole genome shotgun sequence.
ACCESSION   DATALS010000461 DATALS010000000
VERSION     DATALS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441960"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..960
                     /gene="dapA"
                     /locus_tag="VHS06_10255"
     CDS             64..960
                     /gene="dapA"
                     /locus_tag="VHS06_10255"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX2988539.1"
                     /translation="MATFGRVLTAMATPFTSAGEVDYPRARQLALALLDSGTDGLVVT
                     GTTGESPTLSTDEKLRLYAEVKEAVGSRAPVIAGTCNYNTAESIHLSLEAKKAGADAI
                     LGTVPYYNKPTQEGLYQHFKTIAEAVPLPMILYNVPTRTVTNMAPDTTIRLSEVENII
                     GIKEASGNFDGISKIIQRSRSGFQVWSGDDGSTLPILSMGGYGIVSVASHLVGKQIQK
                     MISQFLAGDVASAARMHRDMLPLVNALFVITSPIPLKYALKKVGFDAGPLRLPLIWID
                     PKSAGVVDEALSSATIDLPVQG"
     gene            complement(1177..2205)
                     /locus_tag="VHS06_10260"
     CDS             complement(1177..2205)
                     /locus_tag="VHS06_10260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX2988540.1"
                     /translation="MERVLTLVFLSLLLLVSVAAQKVVGPFSTPATSSLSATSNSAAN
                     SAAAQPNGDKGADSNKGGGEGAAAQGARAFPAESLVSDSRGLWLTDWSRGKVERVDRG
                     PVPRQTALVPIFMYHNVRPIDFKTSNAFVSDLTLPPTEFELQLRQLKERGVSTVTMED
                     LYLYLEGRRDLPSRSVVLTFDDGFENNYLYAMPLLKRYGFVGTFYIISGLVGQNEYMT
                     WREAQEMVSSGMEVGSHTVNHLDLARIPASARESQMVEAKRVLEERLGVSIRTLSYPS
                     GAYNQDAVVAARKAGYVIAVTTQYGTVQDHRKIMELPRVRIHGTDQIAGFRSRLEQIF
                     PSGAPATS"
     gene            complement(2313..>3507)
                     /locus_tag="VHS06_10265"
     CDS             complement(2313..>3507)
                     /locus_tag="VHS06_10265"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX2988541.1"
                     /translation="VMGLAAFAILRLGMGARGQEFALPHGRLVVTWALLLGAGIALSL
                     ASLGRHTSRPALVLIVAVLIADLFMAREPMEYNRPQDSSVYTAQRALPDYLTGLPTAR
                     VLSMVGERYQLADEAELKAKLAGQLTSDGITGYLTAMRLREIVAPNTGMVAGLATVDG
                     YDGGLLPTLRYARLKQALMSGGEYKADQPMRETAGPIPSSRLLGAFGVGYVLAGAERS
                     AQDDGWEDAGTVGASVRLLRNRNLLPRAFVVHRAEVHTDDGDLLAALGHSDLAQVVLL
                     SEDVQFEGVPPDGGERVKVTADRTSSVDITVDMNQPGFLVLSDSYYPGWKVYVDGTES
                     QILRANYTVRAVPLGTGSHQVRFVYDPVSLKLGVGLSLVGTLLVVAAPALVLRRRLSL
                     GRRSI"
BASE COUNT          690 a         1088 c         1077 g          652 t
ORIGIN      
        1 atgtgtcgcc tctactgtag cagagcagta gaagcgataa gcaagattgg agggggtcag
       61 aagatggcga cctttggacg cgttcttacg gcgatggcga ccccttttac cagtgcaggc
      121 gaagtggact accctcgggc ccggcagttg gcgctggcgc tgctggacag cgggaccgat
      181 ggcctggtgg ttaccggcac caccggtgag tctccgacgc tgagcactga cgagaagctg
      241 aggctgtacg ccgaggttaa ggaagcggtg ggcagcaggg caccggtcat cgctggcacc
      301 tgcaactaca acacggccga gagcattcac ctgagcctcg aggcgaagaa agcgggcgcg
      361 gacgccatcc tgggcaccgt gccctattac aacaagccca cgcaagaagg gctgtaccag
      421 cacttcaaga ccatcgcgga agctgtgccg ctgccgatga tcctgtataa cgtcccgacc
      481 cgcacagtga ccaacatggc cccggacacg accatccggc tgagcgaagt tgagaacatc
      541 atcggcatca aggaagccag cggcaacttc gatgggatct ctaagatcat ccaacggtcg
      601 aggtccggtt tccaggtgtg gagcggagac gacggcagca ccctgccgat actctcgatg
      661 ggcggctacg ggatagtcag cgtggccagc cacctggttg gcaagcagat ccagaagatg
      721 atctcccagt tcctggccgg tgatgtggcc tccgccgcgc ggatgcaccg ggatatgctg
      781 ccactggtga atgcgctgtt tgtcatcacc agtccaatcc cgctgaagta cgcactgaag
      841 aaggtggggt tcgatgccgg tccgctgcga ctacccttga tctggatcga tccaaagtcg
      901 gccggcgtgg tggacgaggc actctccagc gccactatcg acctgcccgt acagggctag
      961 caactacaag aacactttcg tagggcgggg tcttgtgccc cgccctcttc gttacctaga
     1021 agccgcttgg tggttgtggc ctcggtgtgg gaggcggggc acaagatccc gccctacgtc
     1081 ttcgtcgacg tggcctgctc cgtcatggcg atcgcttgtc aacgcagggc gccgggcaca
     1141 ggcttggcac ctcgcctctc gtgccctacg tttcttctac gaggtagcag gcgcaccgga
     1201 cggaaagatc tgctccagcc tcgaccggaa gccggcaatc tggtcggttc catggatcct
     1261 cacccggggc aactccatga tcttcctgtg gtcctgaacc gttccgtact gagtcgtcac
     1321 ggcgatgacg tacccggcct ttctcgccgc caccacagca tcctggttgt aggcaccgct
     1381 cgggtaggac agggtgcgaa tcgacactcc cagccgctcc tccagcaccc tctttgcctc
     1441 taccatctgg gactccctgg cagaagcagg gatgcgtgca agatcaaggt gattgacggt
     1501 atgggagccg acctccatcc ccgacgacac catctcctga gcctcgcgcc aggtcatata
     1561 ctcgttttgc cctaccagac cggaaatgat gtagaaggtg ccgacgaagc cgtaccgctt
     1621 gaggagcggc atggcgtaca ggtagttgtt ctcgaaccca tcgtcgaatg tcagcactac
     1681 cgaccgactc ggaaggtctc gcctgccctc gagatagagg tatagatcct ccatcgttac
     1741 cgtggaaacc ccccgttcct tcaactggcg aagctgaagc tcgaactcgg ttggcgggag
     1801 agtgagatcg cttacgaagg cgttgctggt cttgaaatcg atgggacgaa cgttgtggta
     1861 catgaagatg ggcacaagtg cggtctgccg tgggaccggt ccacggtcta ctctctccac
     1921 ctttcccctg gaccaatcgg ttagccacag gcctcggcta tcggacacga gcgactcggc
     1981 cggaaatgcc cttgcacctt gggcagccgc tccctcgccg ccacccttgt tggagtcggc
     2041 acccttatcg ccgttgggtt gcgccgccgc cgaattcgca gcgctgttgg aggttgcgga
     2101 cagcgaagac gtggctggag tcgagaaggg tcccaccacc ttctgggctg ccacgcttac
     2161 gagaagaagt agggagagga acaccagcgt caggacgcgc tccaaggcta ggcaacactc
     2221 cacacgaaag cagtagaagc agaaagcagg gattgcgggg agatcccatg tggggaactc
     2281 gctgccgatt ttaggctact ttacttgagg aatcaaatgg atcgccgccc caaagagagg
     2341 cgacggcgta acacgagcgc cggtgccgcg acaacgagca gcgtgcctac cagcgaaagg
     2401 cctactccta gcttgagcga aacggggtcg tagacgaacc gaacctgatg ggagccggtg
     2461 ccgaggggaa ccgcgcgaac cgtgtagttg gccctcagga tctgcgactc cgtgccgtct
     2521 acgtagacct tccagcctgg gtagtatgag tcggacagga cgaggaatcc aggctggttc
     2581 atgtcgaccg ttatgtcgac cgagctagtt cggtcggcgg tcaccttgac ccgctctccc
     2641 ccgtcaggcg gaacgccctc gaactggacg tcctccgaaa gcagcactac ctgggcgaga
     2701 tcggaatgcc ccagcgccgc gagcaggtcg ccgtcatccg tatggacctc cgctcggtgg
     2761 accacgaatg cccggggaag gagattgcgg tttctgagga ggcgcacgga ggccccgacc
     2821 gtaccggcat cctcccatcc gtcgtcttgc gcgctccgct ctgctccggc caggacgtag
     2881 ccgaccccaa aggcacccag cagccggctg ctcggaatgg gccctgcggt ttccctcatc
     2941 ggctgatccg ccttatactc gccgccgctc atgagcgcct gcttcaggcg ggcgtagcgc
     3001 agggtcggga gaaggccgcc gtcgtagccg tccacggtgg ctagtccggc aaccatcccc
     3061 gtgtttggtg cgactatctc tcgcagtcgc atggcggtga ggtacccggt tatcccgtcc
     3121 gacgtgagtt gtccggccag ctttgccttc agttccgctt cgtctgccag ttggtatcgc
     3181 tcccctacca tactcaggac gcgtgctgtg ggcagccctg tcaggtagtc cggcagcgct
     3241 cgttgtgccg tgtagacgga gctgtcctgc ggccggttgt actccatggg ctcccttgcc
     3301 atgaagaggt ctgcaatcaa aaccgcgacg atgagcacaa gggccggtcg ggaggtatgc
     3361 ctgcccaggg acgcgagaga gagggcgatg ccggcaccga gaaggagcgc ccacgtcacg
     3421 accaatcgtc catggggtag tgcgaactct tgacctctcg ctcccatccc cagtcgcaaa
     3481 atcgcgaaag ccgcaagtcc catcacc
//
LOCUS       DATALS010000462         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_302798, whole genome shotgun sequence.
ACCESSION   DATALS010000462 DATALS010000000
VERSION     DATALS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302798"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VHS06_10270"
     CDS             complement(<1..883)
                     /locus_tag="VHS06_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX2988542.1"
                     /translation="MSTNTGKGGLEGVVAGTSAICEVDGIGGKLSYRGIDIHDLATHS
                     NFEETAYLVWYGQLPTSAQQRELDAQLSCCRDLPHGILRMIIDFPETAEPMMVLRTAV
                     SALSMFDPGGTEHHHDVDLSRAILLTSRVPVIVAAHHRARQRLDPVPPKHSLNLAANF
                     LYMLSGEVPPDHMARTLDRCLILHADHELNASTFAGRVAAATLSDLYSSITAAIGTLA
                     GPLHGGANEQVMMMLRRIGDPAKAEEYVAGLLAEGKKVPGFGHRVYRTQDPRAFELKR
                     MSRAIGEETGETRWYEMS"
     gene            1265..1459
                     /locus_tag="VHS06_10275"
     CDS             1265..1459
                     /locus_tag="VHS06_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988543.1"
                     /translation="MDADSAGFLLDRVDLAREALTEAQQIVAKFAQSEDADISNMLGD
                     ILAQIERLDATVRSGSYPSS"
     gene            complement(1542..1745)
                     /locus_tag="VHS06_10280"
     CDS             complement(1542..1745)
                     /locus_tag="VHS06_10280"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX2988544.1"
                     /translation="MASKHDTSKEKEEGLKGKAEEVMGKAQEEMGKLTGSEKEQGKGK
                     VHETKGKGQQTVAKTKEKVKKAA"
     gene            1993..2853
                     /locus_tag="VHS06_10285"
     CDS             1993..2853
                     /locus_tag="VHS06_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2988545.1"
                     /translation="MAVKSATRRKSRGIAGPWSVIWLRRAVQFYFVLFIAKVAIEKTI
                     AGEESAVFVPSPEAYSPFGGFEGLYKFATTSGLFIAHTHWSNLVLFAGVVLLTLATKS
                     TFCGWICPFGALQEWIGNLGKLLGVKRQAVPSRVDRALRYFKYVVLVWAVTGAATAGV
                     MVFRDVDPYHALVEATAIGFTSSTVVLLITLAASLFVERPWCKYACPLGALIGLMGHL
                     SLVKVEREAASCTGCGLCDRRCPMKVKVSTARRIGSTECNNCLVCTEVCPTGAVQPSF
                     LTLKGVRNEG"
BASE COUNT          507 a          855 c          885 g          652 t
ORIGIN      
        1 cggacatctc gtaccagcgt gtctcccctg tctcctcgcc gatcgccctg gacatcctct
       61 tcagttcaaa ggcgcgaggg tcctgggttc ggtacactcg gtggccgaag cccggcacct
      121 tctttccctc tgcgaggagg ccggccacgt actcctccgc cttcgcgggg tcccctatcc
      181 ggcgcagcat catcattacc tgctcgttgg ctcccccatg cagcggacct gcgagggttc
      241 caatagcggc agtaatggat gagtagaggt ccgacagcgt tgctgcggcg acacgaccgg
      301 caaaggtcga ggcgttcagt tcgtggtccg cgtgaaggat gaggcagcga tccaaggtcc
      361 tggccatgtg gtcgggcggc acttctccgc tcagcatgta caggaaattg gccgccaggt
      421 tgagactgtg cttgggaggg acgggatcca gtctctggcg tgctctgtgg tgggccgcta
      481 ctatgaccgg caccctggat gtgagcagga ttgcccggct gagatccacg tcgtggtggt
      541 gctcggtgcc tccgggatcg aacatggaaa gcgccgagac tgcagtccgc agaaccatca
      601 tcggctcggc cgtttccggg aaatcgatga tcatccggag gatgccgtgc ggaagatcgc
      661 gacagcacga gagttgcgcg tccaattcgc gctgctgtgc ggaagtcgga agctgtccat
      721 accagaccag gtaggccgtc tcctcgaaat tggagtgggt ggcgagatca tgtatgtcaa
      781 ttccccgata cgagagcttt cctccgatac cgtcaacctc gcatatggca gaggttccgg
      841 cgacaacccc ttctaagcct cccttgcccg tgttggtcga cactttctac acccccttgc
      901 gatgttccgt gagtactggt gcaccaacgc atttcttccc tcgcgtcaac gccgaggtgt
      961 cagaattggg cagttgaaat gccatcctga gcaaactgat aatgcaaaaa atgctccaag
     1021 ccctactccc tgcgagcaat acggtcatcg tcttctggcc ttccccatcg cccaatggta
     1081 ggggcggacc tatgtgtccg ccctcgactc cggcgcacac gcaggtgcgc ccctaccgtt
     1141 gtcgggtgct gagtgacacg atgctagggc tttgccggtg ggaaccctgg catggaactg
     1201 gctcctgatc gagcgtgaag tgataggccg gccgctcgga gtccggccgg ggaggtttgt
     1261 cgcgatggat gccgattccg cgggttttct gttggatcga gttgatttgg cccgagaggc
     1321 cctcaccgag gcgcagcaga tcgtggccaa gtttgcacag tctgaagacg cggacatctc
     1381 caacatgctg ggagacatac tggcccagat agaacggctg gacgccaccg tccgaagcgg
     1441 gtcctaccct tcgtcgtgat cacatgaagc tgcccgaggt tcaacgacct cgggcagtga
     1501 agtgagcagc gacagatctg cgggaaacga tcctgcgggt gtcaggcagc cttcttcacc
     1561 ttctccttgg tctttgcaac cgtctgctgc cccttaccct ttgtctcgtg aaccttaccc
     1621 ttgccctgct ccttctcact tcctgtgagc ttgcccattt cctcctgggc cttgcccatc
     1681 acctcttcgg ccttgccctt gaggccctcc tccttctcct tggacgtatc gtgcttggaa
     1741 gccattgctc cacctctatg cttctttggt ttgaggtccc gcacgcctgc aactccggcg
     1801 ggagcgccat aacatggcaa gttcgatgcc cgaaggagag ccggtgtcgg ccgatatctt
     1861 catagaatct ccacacttcc tccataacct caacaaatct gccggctaac atcgtgaatg
     1921 tggaacggtc gcagcgggat tgattgggtg gcggtttgcg atcgtcagtg tgagagcaag
     1981 ggggtagtca aaatggctgt gaagagcgcc acgcgccgca agtcgagggg catcgcgggg
     2041 ccgtggtcgg ttatctggct gcgccgggcg gtgcaattct acttcgtgtt gttcatcgcc
     2101 aaggttgcca tcgagaagac aattgccggg gaggagtcgg cggttttcgt cccctcgccg
     2161 gaggcctaca gcccctttgg gggttttgaa gggctgtaca agttcgccac cacctcaggt
     2221 ctgttcatag cccacaccca ctggtccaac ctggtgctgt tcgccggagt cgtgctgctg
     2281 acgctggcaa ccaagtcgac tttctgcggg tggatctgtc cctttggggc gcttcaagag
     2341 tggatcggca acctgggcaa gctactgggt gtcaagcggc aggccgtacc gagccgcgtg
     2401 gatcgtgcac ttcgatactt caagtacgtg gtgctcgttt gggccgtgac gggcgctgcc
     2461 acggcaggag tgatggtgtt tcgagatgtc gatccttacc acgccctggt ggaggccacg
     2521 gccatcggct tcaccagttc gacggtggtg ttgctgatta ccctggcggc ctccttgttc
     2581 gtggagcgtc cgtggtgcaa gtacgcctgc ccactggggg cattgatagg actgatggga
     2641 cacctcagcc tggtcaaggt ggaacgtgaa gccgcctctt gcaccggctg tgggttgtgc
     2701 gaccggcgct gcccaatgaa ggtgaaggtg tcgacggccc gtcgaatcgg ttcgacggaa
     2761 tgcaacaact gcctggtctg cacggaggtt tgcccgacgg gggcggtaca gccttcgttc
     2821 ctgacgctga agggggttcg caatgaaggt taacccgatc gtttacgctg ccatcgtcgt
     2881 ggtggtgttc tttcttcca
//
LOCUS       DATALS010000463         6377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_302804, whole genome shotgun sequence.
ACCESSION   DATALS010000463 DATALS010000000
VERSION     DATALS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302804"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /gene="glpK"
                     /locus_tag="VHS06_10290"
     CDS             <1..1027
                     /gene="glpK"
                     /locus_tag="VHS06_10290"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX2988546.1"
                     /translation="EAVFGNIDTWVIWWLTGGPNGGAHVTDVSNASRTMLMNLETLDW
                     DPEILKIMGIPRAMLPQIRPSSDPKLYGYALEGQVPVCGDLGDQQAALVGQTCFSTGE
                     AKNTYGTGCFMLLNTGTKPVPSKSGLLTTLGYKMGQEPAVYCLEGSIAITGALVQWLR
                     DNLGMITTSKEVEALAGTVEDNGGIYFVPAFSGLFAPYWMSDARGVIVGMTRYVNKGH
                     IARAALEATAYQTREVLDAMEKDSGVQLTALKVDGGMVYNNLLMQFQSDVLGVPVIRP
                     KVAETTALGASYAAGLAVGFWGNLEETRKNWQVDHTWEPKMAAEQRDKLYKGWLKAVQ
                     RSMHWVE"
     gene            1318..2490
                     /locus_tag="VHS06_10295"
     CDS             1318..2490
                     /locus_tag="VHS06_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2988547.1"
                     /translation="MAPNNFHVLVIGGGSTGAALAHDLALRGLRVTLVERGELTSGTA
                     GRHHGLLHSGGRYAVKDQESARECIEENRILRRIAPGSFEENDGLFVAISEADVAYQA
                     PFLQGCAECGIPTRVLSRADALCCEPYLNPAVKLAVQVPDATLDCWRLPLRFFATAKH
                     NGADIRPYQEVTGIVRRGDTVTGVCLKSHLDGHQYELGADIVVNATGAWAEQVSELAG
                     VRVPVRPSPGVMVAVRGRWCNMVLNRLAPSGDGDIIVPQRSLSVIGTTSWYTEDPDRL
                     GLPEEHVQKMYAKGAELIPALQTAPLRAAWAAARPLIGEETLAGGRELSRTFKAYDHK
                     LRDGVEGIVTISGGKGTTMRGMAELTANLVCQKLGIAAPCRTREVVLLPYWAYYQG"
     gene            2505..3251
                     /locus_tag="VHS06_10300"
     CDS             2505..3251
                     /locus_tag="VHS06_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2988548.1"
                     /translation="MSDNGRPELERPITFKVLRYKPGLIDPPRLDTFTVKVGPLTTVL
                     DALESIRTGQDRTLLYRHSCHHGSCGTCAMKVNHREVLACVTNVLDLKTDEVLVEPLG
                     VLSLMADLTVDMTGFAERYAPAGLQHLRKSEFNPDSSLPEGIAEYRRFENCLECGACL
                     SACPVTNTDPDFMGPAGLAAIYRALVKGEAPPEKLYALADCEHGAWRCHTGWECTAVC
                     PSDNQPAELLMALRRRLLVYKVRRFFGLPA"
     gene            3262..3663
                     /gene="sdhC"
                     /locus_tag="VHS06_10305"
     CDS             3262..3663
                     /gene="sdhC"
                     /locus_tag="VHS06_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX2988549.1"
                     /translation="MTDTPIQHESRWRRALRGWFDPRGGGIGFIAFALNRITGLALVV
                     YLFMHLVVLSSLIRGEAAWTSFLTLARTPLFFFFDVALFAALLYHALNGIRVTLIGLG
                     IGVRLQQALFYGVTALSVVLLAAVTWLMATV"
     gene            3679..4044
                     /locus_tag="VHS06_10310"
     CDS             3679..4044
                     /locus_tag="VHS06_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988550.1"
                     /translation="MQKVMNDRDRKGGTWIWQAASGLGLLLLLGLHMIAQHFVAAGGL
                     RSFQDVLAYIRTPVVMLLEVAFLIVVTYHALLGVRAVVADLGFARRREDTVNRVLTVI
                     GVLVVAYGLYLTWYLALRV"
     gene            4070..4294
                     /locus_tag="VHS06_10315"
     CDS             4070..4294
                     /locus_tag="VHS06_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988551.1"
                     /translation="MAKRKAQPPTKNFRGIPLWGWILIFLLPLVMSEFMFSRAGRTSS
                     AILFPIVWIAFWFVMMMRFGWPMFKKDKER"
     gene            4594..5193
                     /locus_tag="VHS06_10320"
     CDS             4594..5193
                     /locus_tag="VHS06_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2988552.1"
                     /translation="MQSNSFLDHENAERILEEGWLMFQRQGYRGVTIDALCLQCGVTK
                     PTLYYYFHDKENLFVQVLQHKLRGFRKVIEQPGSLDERLQHTALSILESFQTEYSSLL
                     RDRTHIKDPENVERVRVAFHAELFGPLAVLMQAGIDEGNLRGDDPDLLTLVFLGILNS
                     FIGKAIEMHVENAVLAGQLTGFFLDGARLSRLASPTKIG"
     gene            5265..5747
                     /locus_tag="VHS06_10325"
     CDS             5265..5747
                     /locus_tag="VHS06_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988553.1"
                     /translation="MKNLQDLLKRLNPAIPRNWRLTLAGLMWTGVGIMLCSYALTWLR
                     QSVLWIALVVGLLGVAISVLAHRLQFSRLAQKNIDRIRISPERACVFSFLAWKGYLII
                     AVMVTGGMLLRHSAIPRPYLAVIYTAIGGALLQASFSYYFYLARSWQVSAGTLPSRRR
                     "
     gene            complement(5780..6319)
                     /locus_tag="VHS06_10330"
                     /pseudo
     CDS             complement(5780..6319)
                     /locus_tag="VHS06_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
BASE COUNT         1167 a         1831 c         2082 g         1297 t
ORIGIN      
        1 cgaggcggtg tttggcaaca tcgacacctg ggtgatctgg tggctgaccg gcgggccgaa
       61 cggcggggcg catgtgacgg atgtctcgaa tgccagccgg acgatgctga tgaacctgga
      121 gacgctggat tgggatcccg agatcctcaa gatcatgggc atcccgcggg ccatgctgcc
      181 tcagatccgg ccgagctcgg accccaagct gtacggctat gccctggagg gccaggtgcc
      241 ggtctgtggc gatctgggcg accagcaggc ggccttggtg gggcagacct gtttcagcac
      301 cggtgaggcg aagaacacct atggcacggg ctgcttcatg ctgctgaaca ccgggaccaa
      361 gccggtgcct tcgaagagcg ggctgttgac gaccctgggc tacaagatgg gccaggagcc
      421 ggcggtctac tgcctggaag gctcgatcgc catcaccggg gccttggtgc agtggctgcg
      481 ggataacctg gggatgatca cgacctccaa ggaggtcgag gcgctggcgg ggacggtgga
      541 ggacaacggc gggatctact ttgtgccggc cttctcgggg ctgtttgcgc cctactggat
      601 gtcggatgcc cggggggtga tcgtcgggat gacgcgctac gtcaacaagg ggcacatcgc
      661 ccgggcggcc ttggaggcga cggcctacca gacgcgcgag gtgctggacg ccatggagaa
      721 ggattcgggg gtccagctga cggccttgaa ggtcgacggg gggatggtct acaacaacct
      781 gttgatgcag ttccagtcgg acgtgctggg ggtgccggtg atccggccga aggtggcgga
      841 gaccaccgcc ctgggcgcct cctacgccgc cgggttggcg gtggggttct ggggcaacct
      901 ggaggagacg cgcaagaact ggcaggtcga ccacacctgg gagccgaaga tggcggccga
      961 gcagcgcgat aagctgtaca agggctggct caaggccgtc cagcgcagca tgcactgggt
     1021 cgagtaatgg ctgaggattg gctttcctga cagcttgtcc ggccttgcgc atgatctgac
     1081 acatctcttt gtctagtgca ttaggtctac agggtcgcca ccgcggccct gtagacctca
     1141 aaagacccca gttgtcttca cgcctccgcg caagccagga ccgagctcca aatcaatcgt
     1201 tcgttctaca gtccgtcgta gccattgcct gggagggatc atgtccgact ccctggctct
     1261 caagccgcga gcatagccgg gaaggacgcc cttgataggc ccagagaagg agaaacgatg
     1321 gccccaaaca attttcatgt gttggtgatc ggtgggggat cgacgggggc ggccctggcg
     1381 cacgatttgg cgctgcgcgg gctgcgggtg acgctggtgg aacgcggcga gctgacctcg
     1441 ggcaccgccg gccgccacca cggcctcttg cactccggcg ggcgctatgc ggtcaaggac
     1501 caggaatcgg cgcgcgagtg catcgaggag aaccgcatcc tgcgccggat cgccccgggc
     1561 agctttgagg agaacgacgg gctgtttgtg gcgatcagcg aggcggatgt cgcctaccag
     1621 gcgcccttct tgcagggctg cgccgagtgc ggcatcccca cccgggtgct cagccgggca
     1681 gacgccttgt gctgcgagcc ctatctcaac ccggcggtga agctggcggt ccaggtgccg
     1741 gatgcgacgc tggactgctg gcggctgccg ctgcgctttt ttgcgaccgc caagcacaac
     1801 ggggcggaca tccggccgta tcaggaggtg accgggatcg tgcggcgggg cgacacggtg
     1861 acgggggtct gcctgaagag ccatctggac ggccaccagt acgagctggg ggcggatatc
     1921 gtggtcaacg ccaccggggc gtgggccgag caggtcagcg agctggcggg ggtgcgggtg
     1981 ccggtgcggc cgagtccggg ggtgatggtg gcggtgcgcg ggcgctggtg caacatggtg
     2041 ctcaaccggc tggcgccctc gggtgacggc gatatcatcg tgccgcagcg ctcgctgtcg
     2101 gtgatcggca cgacctcctg gtacaccgag gatcccgacc ggctggggtt gcccgaggag
     2161 cacgtgcaga agatgtatgc gaagggggcg gagctgatcc cggcgctgca gaccgctccc
     2221 ctgcgggcgg cctgggcggc ggcccggccg ttgatcgggg aggagacctt ggccgggggg
     2281 cgggagctct cgcggacctt caaggcctac gaccacaagc tgcgcgacgg ggtggagggg
     2341 atcgtgacga tctcgggggg caagggcacg accatgcggg ggatggcgga gttgacggcc
     2401 aacctggtct gccagaagct ggggatcgcg gcgccctgcc ggacgcgcga ggtggtgttg
     2461 ttgccgtact gggcgtacta ccaaggatga ctgcggagga tgctatgtct gataacggcc
     2521 gcccagagct cgagcgcccg attaccttca aagtcctgcg ctataagcct gggttaatcg
     2581 acccgccgcg gctcgatacg tttacggtga aggtaggacc cctcaccact gtactcgatg
     2641 cgctggagag catccggacg ggccaagata gaacgctgct ttaccgtcat tcctgccatc
     2701 atggctcttg cggtacttgt gcgatgaagg tcaatcatcg tgaagtactg gcttgcgtga
     2761 ccaacgtgct ggacctgaag accgacgagg tcctggtgga gccacttggc gtattgtcgc
     2821 tgatggccga tctgacagtc gatatgaccg gttttgccga gcgctacgct cccgccggcc
     2881 tccagcatct gcgcaagagc gaatttaatc ccgactcttc tctgcccgag ggcattgctg
     2941 aataccggcg tttcgagaat tgtcttgagt gcggcgcctg cctctcggcc tgcccggtga
     3001 ctaacaccga tccggatttc atggggcctg ccggcctggc agcgatctac cgggcgctgg
     3061 tcaaaggcga ggcaccgccg gagaagcttt atgctctggc cgactgcgag cacggagcct
     3121 ggcgatgcca tacgggttgg gaatgtacgg cggtctgtcc ttcggacaac cagcctgcgg
     3181 aactcctgat ggcactgcgc cggcgcctgc tggtgtacaa agtccgccgc ttctttggat
     3241 tgcccgcgta aggaagggat catgacagac acgcccatcc agcatgagtc acggtggcgt
     3301 cgcgccctgc gggggtggtt tgacccgcgt ggcggcggta ttggcttcat tgcctttgcc
     3361 ttgaaccgca tcaccggatt ggctctggtt gtttacctgt tcatgcacct tgtagtgctc
     3421 agctcgctca ttcggggcga ggctgcgtgg accagcttcc tgacgctggc tcgcactccg
     3481 ctcttcttct tcttcgacgt ggccttgttt gctgcgctac tttatcatgc cctcaatggg
     3541 atccgcgtta ccctgatcgg cctgggcatc ggcgtacgcc tccagcaggc gctgttctac
     3601 ggcgtgacgg cgctgtcagt cgtgctgctg gctgccgtga cctggctaat ggcaactgtg
     3661 tagaggaagg aacgagccat gcagaaggta atgaacgatc gggaccgcaa gggcggcacc
     3721 tggatctggc aggcggcatc cggtcttggc ttgttgctcc tcttggggtt gcacatgatc
     3781 gcccagcatt ttgtggctgc cggcggcctg cgcagttttc aggacgtgct ggcctatatt
     3841 cgtactccgg tggtcatgtt gctcgaagtc gcgttcctga ttgttgtcac gtaccatgcc
     3901 ctgttgggag tgcgtgctgt ggtggccgat ctggggtttg cccgccggcg ggaagatacc
     3961 gtaaaccggg tgctgaccgt gatcggcgtg ctggtcgtcg cctacgggct ttacctgacc
     4021 tggtacctcg cgctgcgtgt ttgatcacaa cgatgaaagg cggttcctga tggcgaagcg
     4081 caaagcacag cccccgacca agaacttccg cggcataccc ctctggggat ggatcttgat
     4141 ttttctcctc cccctcgtca tgagcgagtt catgttctct agggcaggac gaaccagcag
     4201 cgcgatcctg tttcccatcg tctggatcgc attttggttc gtcatgatga tgcgtttcgg
     4261 ctggcccatg ttcaagaaag acaaggagcg ctagcgcagc gtcgcgtctg gcaagtctcc
     4321 tgtgggccga tataagaccg ggtgggcagt ccgctgccca cccggttctt ttggcgcgcc
     4381 cagcatgggc attgtttaag atgcgccttg ttccccctaa aagatgcggg attggcatat
     4441 gcctatgcaa gccactgggg cttattggac gtaggcccta gagatcctgg acgcatggtc
     4501 cggtcactga tccggacttg acacgccgta tactattcag tatactctat accaattggt
     4561 atacggcgag actggcgcag gagggacgta gcggtgcagt caaattcgtt tctggatcat
     4621 gaaaatgctg agcgcatcct cgaagaggga tggttgatgt ttcagcgaca agggtaccgc
     4681 ggtgtcacca tcgacgcgct ctgtctgcag tgcggagtga ccaagcccac cctatactac
     4741 tactttcatg acaaggagaa cctcttcgtt caggtattgc agcacaagtt gcgcggcttc
     4801 cggaaggtaa ttgagcagcc aggatcgctt gacgagcgcc tgcaacacac ggcactgagc
     4861 atcctggaga gtttccagac tgagtatagc agtttgctgc gggatcgcac tcacataaag
     4921 gatccggaga atgttgagag agtgcgagtg gcctttcacg ctgagctgtt cgggccgcta
     4981 gctgtcctga tgcaagccgg gatcgacgaa ggcaatctga gaggcgacga cccggatctg
     5041 ttgacgctgg tcttcctggg catcttaaat agcttcatcg gcaaggcgat cgagatgcat
     5101 gtggagaacg ccgtgcttgc cggacagttg acaggttttt tcctggatgg agcaagactc
     5161 tcaaggttgg catctcctac gaaaatcggg tagctaagca cccatactga agcaattcat
     5221 gtgcgctgat tggagcggtt caaggcgaca taaggaggcc cggcatgaag aacttgcagg
     5281 acttactgaa acggctaaac cccgcgatcc cgaggaactg gcgattgacc ttggccggat
     5341 tgatgtggac cggcgtcggc atcatgttgt gcagctatgc cctgacctgg ctcaggcagt
     5401 ctgttttgtg gatcgctctc gttgtcggtc tgctgggtgt ggcgatctcc gtgctggccc
     5461 atcggctgca gttttccagg ctggctcaga aaaacatcga tcgcattcga atctcacccg
     5521 agagagcttg tgtcttttcc tttctggcct ggaaaggcta tctgatcatt gccgtgatgg
     5581 tgacgggcgg catgctgctc cgccactcgg ccatcccccg cccttatctg gcggtcatct
     5641 acactgcgat tggtggcgca ctcctccagg cgagtttcag ctactacttc tacctggcac
     5701 gctcgtggca ggtgtctgct ggaaccttac cgtccaggcg cagatagatg cttgggagac
     5761 gctcagccct gatgtggtac tagcgacttg ctgcctctcg gtccagccta cgatcggtag
     5821 gaatcaccgt ctcagctacc ctcttcgcgt taacctcttt ccacagccgt cgtccaacca
     5881 tcggcatcac aaccgccact accgatatgc ccacgtattg aatcgggaac atcacagcca
     5941 tcaagcccaa tacgatcagc ggtttcggca acacaacgac cagcagagca gccatcactg
     6001 ccaatacagc cgccggggca ggaacggatg ggaacagtac cgtgaccgaa agcccgagcg
     6061 cggcgctgct gaacatgatc ggcaagaact gtccaccctt ccagccgctc gccagcagca
     6121 ggttgaccag gaatatcctg gcgagggcga tcaggagcag cgcccagaat cccagttggg
     6181 cggcctgttc gtagacttgc tgcaattgat gttgcccaga gaattgcagc atcggcacaa
     6241 atagtgccat cagccccaag gtgaatccgc caagaaccgc tcgcaggact ggcctgtgcc
     6301 ggattggcgc cacccaccgt ttcgtccagg tctctagggc ctacgtccaa taagccccag
     6361 tggcttgcat aggcata
//
LOCUS       DATALS010000464         4631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_71644,
            whole genome shotgun sequence.
ACCESSION   DATALS010000464 DATALS010000000
VERSION     DATALS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71644"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /gene="hydA"
                     /locus_tag="VHS06_10335"
     CDS             <1..1394
                     /gene="hydA"
                     /locus_tag="VHS06_10335"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX2988554.1"
                     /translation="LAVHDRQHRLDRRGAEEDVLELLSRHALVAISDGTIAAILAPGA
                     LPEGAAQAKRVDASGKVVMPGVVDAHVHMRNFNLQADSYVSMSTCAAFGGVTTVIPYQ
                     QGKVGMPVKEFLTTLRDEGEAESLVDFAMHCRLQAPERGVTKQFADAFDLGVTSFKMF
                     MAYRKRGIMWDDYSLYEALEYIGKNGGMFCCHAENGDIIDYLEDKYTAEGRYTPENYL
                     AVRPPSAEAEAAFRVLTISKLANCTLYLVHMSTGRGIEIAHQAQLQGQRVFIESCPHY
                     MTLTCEEMKRQGGKTKIAPPLREQHDLDAVWQAVMDGVVQVVGSDHAPWPMDNKSMPA
                     EKFKDIFFGAPTIETMLPIMYSEGVAKGRIDLPRLAELLCEGPAKIFGLAGKKGSISV
                     GADADLVLIDPNAEWKVDGKAMHSEAGYSPYEGWKMKGKVAATMLRGEFIMQDGKLLK
                     SPGYGKYIPREAK"
     gene            1520..2767
                     /locus_tag="VHS06_10340"
     CDS             1520..2767
                     /locus_tag="VHS06_10340"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEX2988555.1"
                     /translation="MEPERLRVVASKVQIDRVMERIHLLADKARGTGGGVDRLAFSPV
                     EAEAMSLVAGWMQDAGLTVSYDGIGNMYGSSDGNAAGAAVMMAGSHLDSVPNGGPYDG
                     VLGVIGAVEAVEAMRAAGIRPSKPLEIVVWRCEEAALFGQGRLGSLFFTGELSMETLR
                     RWATDENKLLSYLERAAALPQRAAGRKLAGYLELHIEQGRRLEAGQTTIGVVTAIPGA
                     IRWRINLKGRADHSGATPMGMRRDALAAAAELVLAVERAGMEEKSHETVATAAAITAL
                     PGAWNVVPGTAQILTDIRGIEEESVARALAFVKDHARGIGARRGVEITFEEATRGVPV
                     KLDPSMVARVDETAAAVGHSAVRMPSGAGHDAQTIAPYAPVGMVFVPSIDGISHAPAE
                     QTKPKDIEAGVQVLAGTWASLAE"
     gene            complement(2842..3714)
                     /locus_tag="VHS06_10345"
     CDS             complement(2842..3714)
                     /locus_tag="VHS06_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2988556.1"
                     /translation="MSQGGQQKQRTEPPQQQKRQPGIESEMQPKPKSEEQEYRGTGKL
                     AGKVALITGGDSGIGRAVAILFAKEGADIAIAYLDEHEDAAETKRQVEEEGRHCITMA
                     GDVGEEQHCFELVNQTVDELGRLDILVNNAAVQYPQESIEKISSDQLIRTFTTNIFSY
                     FYMVKAALPQLGEGSAIVNTTSVTAYKGQPMLLDYSSTKGAIVAFTRSLALSLVGKGI
                     RVNGVAPGPIWTPLIPSSFPPEKVETFGGDVPMKRPGQPEEVANCYLFLASDDASYMT
                     GQVLHPNGGTIVNG"
     gene            4019..4609
                     /locus_tag="VHS06_10350"
     CDS             4019..4609
                     /locus_tag="VHS06_10350"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX2988557.1"
                     /translation="MTRPISRRKFLKLSAGSTGAAAMVELAGLGFDAALVRAQIGTGL
                     TKQGKEIPTVCPYCAVGCGQIATVADGKLIDRQGNPESPINEGTLCPKGAASYQLAVN
                     SNRWTKAKYRAPGSDHWEDISLDDAMDRIARKFKETRDGNFIEQVKVGDKQKTVNQVL
                     AVASLGGATIDDEWNYLHQKLFRAMGDVSIENQARI"
BASE COUNT          852 a         1359 c         1520 g          900 t
ORIGIN      
        1 ccctcgctgt acatgatagg cagcatcgtc tcgatcgtag gggcgccgaa gaagatgtcc
       61 ttgaacttct cagccggcat gctcttgtcg ccatcagcga cgggaccatc gcggcaatac
      121 tggcaccggg cgcgctgccg gaaggcgcgg cgcaggccaa gcgggtcgat gccagcggca
      181 aggtcgtcat gccgggcgtc gtggatgcac acgtccacat gcggaacttc aacctccaag
      241 ccgactccta cgtcagtatg agcacctgcg cggctttcgg tggcgtcacc acggtgatcc
      301 cttaccagca gggcaaggtg gggatgccgg tcaaggagtt cctcaccacc ctcagggatg
      361 agggcgaagc cgagtcgctg gtcgacttcg ccatgcattg ccggctccag gcgccggaga
      421 ggggcgtgac caagcagttc gcggacgcct tcgacttggg cgtgaccagc ttcaagatgt
      481 tcatggccta ccgcaagcgt ggcatcatgt gggacgacta ctccctgtac gaggctctgg
      541 agtacatcgg caagaacggt gggatgttct gctgccacgc cgagaacggc gatatcatcg
      601 actacctcga ggacaagtac acggccgagg ggcgctacac ccccgagaat tacctggctg
      661 ttcgtcctcc ctccgcggag gctgaagcgg ccttccgtgt tctgaccatc tccaagctgg
      721 cgaactgcac cctctacctg gtacacatga gcaccggtag gggcatcgag atcgctcatc
      781 aggctcagct gcaggggcag agggtgttca tcgagtcctg cccgcactat atgacgctga
      841 cctgcgagga gatgaagcga cagggcggaa agacgaagat cgcgcccccg ttgcgggagc
      901 agcacgatct ggatgcggtc tggcaagccg tgatggatgg tgtcgtccag gtagtgggct
      961 ccgaccacgc gccgtggccg atggacaaca agagcatgcc ggctgagaag ttcaaggaca
     1021 tcttcttcgg cgcccctacg atcgagacga tgctgcctat catgtacagc gagggcgtag
     1081 caaagggccg catcgacctg ccgaggctgg cagagttgct ttgcgagggg ccggccaaga
     1141 tcttcggcct ggctggcaag aagggcagca tctcggtggg cgcggacgcc gacctggtgc
     1201 tgatcgaccc caacgcggag tggaaggtcg acggcaaggc gatgcacagc gaggccggct
     1261 actcccccta cgagggctgg aagatgaagg gcaaggtggc cgccaccatg ctgcgcggcg
     1321 agttcatcat gcaggacggc aagctgctga agtcgccggg ctacgggaag tacatcccgc
     1381 gcgaggccaa gtagcagtct tttcgcgaca tcctggcccc aggtaggggg ccgtgtagtt
     1441 gaggcgtagg gcggggtttt gtgccccgcc tttcgcatct gtgtcgcctg ttgaccattt
     1501 atctcatggg ggtcttgtta tggagcctga gcgtctgcgg gttgtggctt ccaaggtaca
     1561 gatcgatagg gtgatggaga ggatccatct tctggcggac aaggcgaggg gcaccggtgg
     1621 tggagtggac cgcctcgctt tctctcccgt cgaggccgag gcgatgtcgc tggtggccgg
     1681 ctggatgcag gatgcaggtt tgacggtgtc ctacgacggt ataggcaaca tgtacggctc
     1741 cagcgacgga aacgccgcgg gggcagcagt gatgatggcc ggttctcacc tggactccgt
     1801 gccaaacggc ggtccgtacg acggagtgct gggtgtcatc ggcgcggtcg aggcggtcga
     1861 ggcgatgcgt gctgccggca tcaggccatc taagccgctg gagatcgttg tgtggcgttg
     1921 cgaggaggcg gcgctgttcg ggcagggcag actcggttcg ctgttcttca ccggcgagct
     1981 gagcatggag acccttcgcc ggtgggcgac cgatgagaac aagctgctct cgtacctgga
     2041 acgggcagcc gctctgccgc agcgggcggc gggccggaaa ttggccggat acctggagtt
     2101 gcatatcgag caggggcgcc ggttggaggc gggccagact accatcgggg ttgtcaccgc
     2161 aataccgggc gccattcgct ggcggatcaa cctgaagggg cgcgccgacc actctggagc
     2221 cactcccatg gggatgcgtc gggatgcact ggcggccgct gccgagttgg tgctggcggt
     2281 ggagcgcgcg ggcatggagg aaaagtctca cgagaccgtt gccaccgcgg ccgctatcac
     2341 cgcattgcct ggtgcctgga acgtcgtccc cggtaccgca cagatactga ccgatatccg
     2401 tggcatcgaa gaggagagcg tggccagggc gctggccttc gtcaaggatc acgccagggg
     2461 tatcggcgct cggcgaggtg tggaaatcac cttcgaagag gccacgaggg gtgtgcctgt
     2521 gaagctggac ccgtcgatgg tggcgcgagt cgacgagacg gcggcggctg tcgggcacag
     2581 cgcggttcgc atgccgagtg gggcagggca cgatgcccag accattgccc cctatgcccc
     2641 ggtgggcatg gtgttcgttc cttcaatcga cggcattagc catgcgccgg cagagcagac
     2701 taagccgaag gatatcgagg cgggggttca ggtgctggcc ggcacttggg catcgctggc
     2761 cgagtaattt gtagcgaaac ggcagaatag tggcaattcg gggtggggca gtctgggacg
     2821 tcctgccccg aattgcgtgt tctacccgtt tactatcgtt ccgccgtttg ggtgaagcac
     2881 ctgcccggtc atgtaggaag cgtcatctga ggccaggaac aggtagcagt tggccacctc
     2941 ctccggctgc cccggacgct tcatcgggac gtctccgcca aaggtttcca ccttctcggg
     3001 cggaaaggag gaggggatga gcggtgtcca gatgggaccc ggcgcgacgc cgttgaccct
     3061 gatccccttg cccaccagtg acagcgccag tgatcgggtg aaggccacta tcgcaccttt
     3121 ggtcgatgag tagtcgagca gcatcggctg ccccttgtag gcagtgacgg aggtcgtgtt
     3181 cactatggcg ctgccctctc ccagttgggg cagcgcggcc ttcaccatgt agaagtagct
     3241 gaagatattg gtggtgaagg tgcggatcag ttggtccgag ctgatcttct cgatgctttc
     3301 ctgcgggtac tgtacggcgg cgttgttcac caggatgtcc agtcgcccca gttcgtcgac
     3361 cgtttgattc accaactcaa agcagtgctg ttcttcgccg acatctccag ccattgtaat
     3421 gcagtgccgc ccttcctctt cgacctgcct cttagtctcg gcggcatcct cgtgctcatc
     3481 caggtaggct atggctatat cggccccctc tttggcgaag aggatggcta cggctcggcc
     3541 tatcccactg tcgcctccgg tgatcaaggc caccttcccg gccagtttgc ccgttccccg
     3601 atattcctgc tcctcggact tcggcttagg ctgcatctcc gactcgatgc ccggctgcct
     3661 tttctgctgc tggggcggct cggttcgctg cttctgctgt ccaccctgcg acacggctct
     3721 ctccaatcca acccaactcg gatatgttcc ggctccatcc ggagtggctt cattcgtggg
     3781 atggaacgaa ggcaagtcat gtgcccttag ccgcatggcc ctccgattct ccatacgcct
     3841 gcctcagaat gcgtcctttc ctggcacgga gcgtgcgcta gaacatcttg cgagtacctt
     3901 acagtgctgc aatcttggcc gaactgcgta gtttgacctg cttcaatcga cttgccggct
     3961 accctcgctc agatgctgac acggcgaagt gaaatccttc cgaaaggagt ggtgccagat
     4021 gacccggcct atctctcgtc gcaagttcct gaagctgtcg gcaggttcga caggtgccgc
     4081 cgctatggtg gagcttgcag ggcttggttt cgatgccgcg ctggtccgcg cgcagatcgg
     4141 aaccgggctc acgaagcagg gaaaagagat cccaaccgtc tgcccctact gtgccgttgg
     4201 ctgcggccag atcgcgacgg ttgccgatgg gaagttgatc gacagacagg gcaatcctga
     4261 gagccccatc aacgagggaa ccctttgccc caagggtgcc gccagctatc aacttgccgt
     4321 caactccaat cgttggacga aggcgaagta cagggccccc ggctccgacc actgggagga
     4381 catctccctg gatgatgcca tggaccggat cgccaggaag ttcaaggaaa cccgtgacgg
     4441 caacttcatc gagcaggtaa aggttggcga taagcagaag acggtcaatc aggtactggc
     4501 agtagcctcc ctgggtggcg caacgataga cgacgagtgg aactacctgc accagaagct
     4561 gttccgcgcc atgggagacg tgtctatcga gaatcaggcg cgcatttgac acagcgcctc
     4621 ggtccccggt c
//
LOCUS       DATALS010000465         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_119684, whole genome shotgun sequence.
ACCESSION   DATALS010000465 DATALS010000000
VERSION     DATALS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119684"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VHS06_10355"
     CDS             <1..869
                     /locus_tag="VHS06_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEX2988558.1"
                     /translation="HDVFVLDFMNDADTITDAFSEYYRATLLSEETDPNKLHDLQGQL
                     DAHHVYTPEQIEAFVTLYLAGADRDKLDPILDTCVAVYKEHLDEDQQVDFKGKAKSFV
                     RTYDFLASVLPYTNAGWEKLSILLNFLVPKLPAPKEEDLSKGILESIDMDSYRVEKRA
                     TIAVPLPDEDAEIGPIPVGGGGRKAEPELDRLSSILKTFNEQFGTLFTDADRIIKRIH
                     EDIAPKVGADAAYQNAKSNTPDAAKIELDAALMRVINPLLRDDTEFYRQFVQNESFKR
                     FVTETVRGLTTG"
     gene            complement(1555..2445)
                     /locus_tag="VHS06_10360"
     CDS             complement(1555..2445)
                     /locus_tag="VHS06_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2988559.1"
                     /translation="MDDELLGALYHYLFSQDNWRSTAFHVYSDALMVLIELFRVGRQL
                     SQRQALNRKHWPLWTRRLKFPSYSQFRRRVAAAPVQQILEQLGTHFRQQLPCTQEKVC
                     DGKPLLVGGYSKDPDAHTGKAPGGWAKGYKLHAIVDAAGAVETWAVTALHVGEGGMAR
                     QLVGRTPLKQVLLRADANYDSVELYRSVAEAGGRLLAPRRKPGTGLGHQCQQHEHRLQ
                     AIAELEGSAAALKVFERHRIRIEQVFGHLHNAPFGIWGLPSHVRRLPRVERWIRAKLA
                     LYHLYLVLQQLHRQPSAEAA"
     gene            complement(2498..3103)
                     /locus_tag="VHS06_10365"
     CDS             complement(2498..3103)
                     /locus_tag="VHS06_10365"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988560.1"
                     /translation="MLDVARRLNHEVADVSFRVPTTMQTHPIVSELITRHSLGRFRLK
                     GDKITAVQDSFDKMVPECDLCLTVSGTATLHVASFGVPMIVVYRASPILWNLIGRWLI
                     KTRTFSLVNLLADDNRLVPEYVPWYGSNKPVFLHALDYLRNPELLASQRKRLLDLITR
                     LDKEGASTNTAKIALELMATATQSVSTPSPSAGLITRRAKG"
     gene            complement(3007..>3305)
                     /locus_tag="VHS06_10370"
     CDS             complement(3007..>3305)
                     /locus_tag="VHS06_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988561.1"
                     /translation="HDVFVLDFMNDADTITDAFSEYYRATLLSEETDPNKLHDLQGQL
                     DARRSSACFPARAAAKPPPISPICSTSPAGSTTKSPTSASASPPPCRRTPSSQS"
BASE COUNT          617 a          886 c         1191 g          611 t
ORIGIN      
        1 agcatgatgt gtttgtgctg gacttcatga acgacgcgga cacgatcacc gacgccttct
       61 cggagtatta ccgcgctaca ctgctgagtg aggaaaccga cccgaacaag ctgcatgatc
      121 tgcaggggca actggacgcc caccatgtgt acacgccgga acagatcgag gcgtttgtga
      181 cgctgtacct ggccggggca gaccgggaca agctcgatcc aatcctcgat acctgtgtgg
      241 cggtgtacaa ggagcacctg gacgaagacc agcaggtgga cttcaagggg aaggcgaagt
      301 cgtttgtccg cacgtatgac ttcctggcat ctgttctgcc ctacaccaat gcagggtggg
      361 agaagctgtc gatcctgctg aacttcctgg tgccgaagct gccggcgccg aaagaggagg
      421 acctgtcgaa aggaatcctc gaatcgatcg acatggacag ctaccgggtt gagaagcggg
      481 caacgatcgc ggtgccgttg ccggatgagg atgccgagat cgggccgatt ccggtgggcg
      541 ggggtgggcg caaggcggag ccggaactgg atcggttgag cagcatcctg aagacattca
      601 acgagcagtt cggaacgctg ttcacggacg ccgaccggat catcaagcgc attcacgagg
      661 acatcgcgcc gaaggttggg gcggacgcgg cgtaccagaa cgcgaagagc aatacgccgg
      721 atgcggcgaa gatcgaactg gatgcggcct tgatgcgtgt gatcaacccg ctgcttcggg
      781 acgatacgga gttctatagg cagttcgtgc agaacgaatc gttcaagcgg tttgtcaccg
      841 agacggttcg cgggctaacc acggggtgag gccggcgact gatcgctgta tgtgtgagtg
      901 tgttgattgt tgattgacgg aggggaacgc tgcgctggga gatagtggga cgctgcgggc
      961 catggttcct gtggagatcg aggggcgtaa gcgagtacgc accctcatcc ccgacccttc
     1021 tcccagaggg agaagggggg cacggtgctg ttgtgcatct gccatggggc gaagggggag
     1081 ggtaggatgg ggagcatggg agcgagtggg cattaccgtg gccgattgcg gtttgctgga
     1141 ctgtcgccgg tggcgcggga gatgcgtcgg cgggagacgc tggccgagga gatcctctgg
     1201 gcccggctgc gcggccggcg gcttggcggg gtgaagttca gaaggcaaca ccagtttggg
     1261 ccgtatgtgt gcgacttcta ctgcgatgca gtacggctcg tggtggagtg cgatggcggc
     1321 gtgcatgggc agtatcgtgt caggaaacat gattgggctc gcgagcggta catgcggtcg
     1381 atggggctga tggtggtgag ggtcagcaat gagcaggtgc ttgaggatgt ggagcgggta
     1441 cttgcgagga ttgaggagag cgtgaagggg aggatgaagg cgaaggagcg gggatggtat
     1501 gccgggtacg gcaccctcat cccccagccc ttctcccaga gcaggattga tcatctaggc
     1561 agcctcagca gaaggttgcc ggtggagttg ctgcaggacg aggtacaggt ggtagagcgc
     1621 cagcttggct cggatccagc gctctactcg tggcaggcgc cggacatgcg aaggcagacc
     1681 ccagatcccg aacggagcgt tgtgcagatg cccgaacacc tgctcgatgc ggatgcgatg
     1741 ccgctcaaag acctttagcg ccgcggcact gccttccagt tcggcaatcg cctgcaggcg
     1801 gtgctcgtgc tgctggcact ggtggcccag gccggtcccg ggcttgcgac gcggagccag
     1861 gagccgacct ccggcctcgg caacgctgcg gtagagttcc acgctgtcgt agttggcatc
     1921 ggcccgcagc agcacctgct tgagcggcgt acgcccgacc aactgcctgg ccatgccgcc
     1981 ttcccccaca tgcaacgccg tcaccgccca ggtctccacc gccccggcgg catcgacgat
     2041 ggcgtggagc ttgtagccct tggcccagcc gccgggagct ttgccggtgt gggcatcggg
     2101 atccttggag tagccgccca ccagcagcgg tttgccatcg cagaccttct cctgcgtaca
     2161 gggcaactgc tgacggaaat gggtgccaag ctgctccagg atctgctgca ctggggcagc
     2221 ggccacacgg cggcggaact ggctgtagct ggggaacttc agccgtcgcg tccacagcgg
     2281 ccaatgcttg cggttcaggg cctgccgctg gctcaactgc cgccccaccc ggaacagctc
     2341 aatcaggacc atcagtgcgt ccgagtagac atggaaggcg gtgctccgcc agttgtcctg
     2401 ggagaagaga taatgataga gtgctccaag cagttcgtcg tccatgctgc ggtcctttcg
     2461 atgacaaccg cattgtggcg gcgaactgct tgtcttgtca gcccttagct cgcctcgtga
     2521 tcaatcctgc agagggagag ggagtcgata cactctgggt ggcggtggcc attagctcca
     2581 gggcgatctt ggccgtgttg gtgctggcgc cttctttgtc gaggcgggtg atcagatcga
     2641 gcaggcgctt gcgctgggag gcgagcagct ccggattgcg cagatagtcc agggcgtgca
     2701 ggaagacggg cttgttcgag ccgtaccagg ggacgtactc ggggacgagg cggttgtcgt
     2761 cggccagcag gttcaccagc gagaaggtgc gggtcttgat cagccagcgg ccgatcaggt
     2821 tccagaggat tgggctggcg cgatagacga cgatcatcgg gacgccgaag ctggcgacgt
     2881 gcagggtggc ggtgcccgag acggtcaggc agaggtcgca ctcggggacc atcttgtcga
     2941 agctgtcctg gacggcggtg atcttgtcgc ccttgaggcg gaaacggccg agcgagtggc
     3001 gggtgatcag ctctgagacg atggggtgcg tctgcatggt ggtggggacg cggaagctga
     3061 cgtcggcgac ttcgtggttg agccggcggg cgacgtcgag cagatggggg aaattggcgg
     3121 tggcttcgct gcggcgcgag ccgggaagca ggccgacgat cggcgggcgt ccagttgccc
     3181 ctgcagatca tgcagcttgt tcgggtcggt ttcctcactc agcagtgtag cgcggtaata
     3241 ctccgagaag gcgtcggtga tcgtgtccgc gtcgttcatg aagtccagca caaacacatc
     3301 atgct
//
LOCUS       DATALS010000466         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_303473, whole genome shotgun sequence.
ACCESSION   DATALS010000466 DATALS010000000
VERSION     DATALS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303473"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            716..979
                     /locus_tag="VHS06_10375"
                     /pseudo
     CDS             716..979
                     /locus_tag="VHS06_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357371.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
     gene            1042..2070
                     /locus_tag="VHS06_10380"
     CDS             1042..2070
                     /locus_tag="VHS06_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988562.1"
                     /translation="MGTLGNSSKFNLMRVTEQLPLSANSLTSYLQHEFARLSPPDWHC
                     SREVSLLAPDLADLLGYAPRADVLLEQLDGTSRLWIEFEVSRADPVANHAKFATAPLF
                     QPRSRGDAFISMVSPHVARGRRNLAANTIQVMRQIGMNAFQTVLFPHMSGLEVQRLNH
                     LSRDSLTEERPEVEPEFERALAVSQPILTQDDCRLHFAGDITEVMLNLRQWNDEILTT
                     RGAQQWGRRTVTYFVHNPVKRYFAPSKFCAYVPVPLFLDGITSRPGGIGAMTLGVYAN
                     LDRATKIFDGTRARTHLTKRLGLLECTPMDHPEIAFSFELWLSKHNDAITVSPSGPVF
                     LVPPSWFA"
     gene            complement(2130..>2387)
                     /locus_tag="VHS06_10385"
     CDS             complement(2130..>2387)
                     /locus_tag="VHS06_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988563.1"
                     /translation="EGGKRPLTEFPRLRLTWRGCCTEEVRTESFRGENFSPTMVYQFK
                     QVLWQAGILATKADRNAGKDAGSYRPDDDVWALELRLILRA"
BASE COUNT          478 a          764 c          635 g          510 t
ORIGIN      
        1 gggacagggg caccaggatc ttgcacttgc gagaacagta tcgcgggggc ggctggtatc
       61 cgtcaaggtg gatttgagcg gcggaggctg ggagttcttg aggttctttt gcatagtggg
      121 ggcggggaat ctacgacaat ctgtacgaca atgcggatga gggagagtga agacaaacac
      181 ctttacgggc ggatacacgc gtcgaatcgg cctccagcca ttgtagatct acgggtgatg
      241 gaggggcgtg aggcgccgag aggaggtttc gtaaaccgcc ggtcgccagt tcgaatctgg
      301 ccgtcggctc tatgcagaag gagtctccca cgcacggagg ctcctttgtt tatgcgcatt
      361 tctcaggttg cttgaggtcg tgcacgcctt gtacaacaat cggtacaaca accccgccac
      421 gtgagcctca gtctaggaaa ctcggtgagg ggccgctttc ctccttcgcc gtgcgtggtt
      481 ctacgcatgc tcgggccaga cttagccctt tttagacccc ttgtcgccac ccctccacac
      541 caatccacac cccaattgat gtactggttg ccccggcctc ttcttcgacc gacaccgtta
      601 aaattatccc actgtcacct tgacggaatc cccctcccca accatactat gctgccaagt
      661 gcaagatcca gctgttcctc gagtattcgg gaacacccct caggagttgt ccctggtgcc
      721 taccactaac acgcgagctg tcgtcctcct cagcggagga ctcgactcca caaccaccct
      781 ggccttgcgg ggaaccaggg ctacgaagtc tatgccctca ccttcagcta cggccagcgg
      841 cacgaagtgg aaatcgaggc ggcgcagaag atcgcagctt ccatggtcgt agcccgccac
      901 gtggtagccg gtatcaacct ccgtctcttc gtcggatctg ccctcaccga tggacatagc
      961 gctaccggca catccgtccg gcaatccacc tgatgccgtc agcacaggtc tcatatctgg
     1021 ctcttccagc acatcgacca aatgggtacg ctcggcaata gtagtaagtt caacttgatg
     1081 cgtgtgacgg agcagctgcc gttgagtgcc aattccctca caagctatct gcagcacgag
     1141 ttcgcaagac tttctccccc cgactggcat tgttcacgtg aagtctcgct cctcgcgcca
     1201 gacttggccg acctcctggg ctatgctcct agagccgatg tgcttctgga gcaattagat
     1261 gggacaagtc ggctctggat agagttcgag gtgagtcggg ccgatccagt tgctaaccac
     1321 gccaagttcg ccaccgcccc tctcttccag cctcggtccc ggggagacgc cttcatctcc
     1381 atggttagcc cccatgttgc ccgtggacgc aggaacctcg cagccaacac catccaggtc
     1441 atgagacaga tcgggatgaa cgctttccaa actgtcttgt tcccgcacat gtccggcctc
     1501 gaagtgcaga gactcaacca tctgagtagg gatagtctga cagaggaacg ccccgaggtg
     1561 gaacccgaat tcgaacgggc gttagccgtg tcccagccga tactgaccca agacgactgc
     1621 cggctgcatt tcgctggtga catcaccgag gtgatgctga acctgaggca gtggaacgat
     1681 gagatcctca cgacgcgagg tgcacagcag tggggccggc gcacagttac ctacttcgtg
     1741 cacaaccccg tcaaacgcta cttcgcgccg tcgaagttct gcgcttacgt gcctgttccc
     1801 ctgttcttag acgggatcac gagcaggcca ggtggaatcg gggcaatgac tttgggcgtc
     1861 tatgcgaatc tagacagggc gacgaagatc ttcgacggga ctcgcgctcg gacacacctc
     1921 accaaacgcc tcggcttgct tgagtgcact cccatggacc accctgagat cgcattctcc
     1981 ttcgagctct ggctgagcaa gcacaatgac gccatcacgg tctctccgtc aggacccgtg
     2041 ttcctcgttc cgccatcctg gttcgcgtag ctgtcgccct ccccgcacag ctggccagca
     2101 ccgcctaacg tttccatcga tggctcactc tacgctctca gtatcagccg cagctccaat
     2161 gcccacacgt cgtcgtccgg tcggtacgac cctgcatcct tccccgcgtt ccggtccgcc
     2221 tttgtcgcta gaattcccgc ctgccacagc acctgtttga attgataaac catggtcggg
     2281 ctgaaattct cgcccctgaa tgactcggtc ctcacctcct cggtacaaca accccgccac
     2341 gtgagcctca gtctaggaaa ctcggtgagg ggccgctttc ctccttc
//
LOCUS       DATALS010000467         8880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_72067,
            whole genome shotgun sequence.
ACCESSION   DATALS010000467 DATALS010000000
VERSION     DATALS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72067"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VHS06_10390"
     CDS             complement(<1..923)
                     /locus_tag="VHS06_10390"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX2988564.1"
                     /translation="MSIRLRLALWYTAVLGITLVVFSVLLYMLMEYHLINESDDSIIS
                     RAQHIASTLRVETGASSPSSPSLGQVALPPIDAFESPGIYVQVMQLDGFVVARSDNLG
                     DQQLPIGAQDLVAVRRGNSALYTVDVSGQQVRVYAQPLGAEGKLVGIVLVARTYSDAF
                     ETLYQLRSWLLLAGGISLLLAGAIGWAIAGSALRPIATMTRTARAIALSKGFSRRLED
                     TNSRDELGQLGLTFNEMLASLEDAYNTQQRFIADASHELRTPLTAVRANLELLEKQGE
                     ALPPADRHELAAAAASEANRMSRLVEELLSLA"
     gene            complement(930..1610)
                     /locus_tag="VHS06_10395"
     CDS             complement(930..1610)
                     /locus_tag="VHS06_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2988565.1"
                     /translation="MAERILVVDDDPGVTSVLKRGLGYEGYEVVVAGSGREALDLARG
                     NPPDLVILDIMMPGMDGLEVTRRLRAVDGKLPILLLTAKDAPADQVTGFETGADDYVV
                     KPFVFEVLLARVRALLRRREPEEESVLRYKDLALDTGSRVARRGGREISFTTTEYELL
                     HLFLQHPEKVLTRDSIMHRVWGYDFSGDYNILEVYVRYLRSKLEEKGEPRLIQTVRGA
                     GYVLRDRE"
     gene            complement(1901..2713)
                     /gene="lgt"
                     /locus_tag="VHS06_10400"
     CDS             complement(1901..2713)
                     /gene="lgt"
                     /locus_tag="VHS06_10400"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX2988566.1"
                     /translation="MGPIVVDIDPVIIGVGHFALRWYAVGIMLGILAAFTIAAREARR
                     RGLDTDEIYNMGLWAVLGGLAGARLLHVIDNWGYYSANPGSIIAIQQGGLAILGGILG
                     GTVVAVVYIARRGLPLGKIADAVAPGMILGQAIGRIGCIVNGDAVGAPTSLPWGLVYT
                     NPGAMAPSLGDAYQPTQVYEMLWDLLVFGLLWWARGKVKLEGGLFLAYLGLYSFGKFV
                     ISFWRQEALFLLGLQEAQVVALASIAGAGALAYHLWARQKALPAAVASGPAE"
     gene            3123..4217
                     /locus_tag="VHS06_10405"
     CDS             3123..4217
                     /locus_tag="VHS06_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2988567.1"
                     /translation="MAQITLKAGGPKARHVIAIASATLLLLLPIGLRVAFPQEHIALL
                     YALPILVAGQSLATPLAVLVALLALAGHSVDSLIDGSSVWTWLIQGTILLIVAYLSVR
                     TRQEQTELARLAGELQHVHIQQERFVSSLAHDIRGGITVILGYAQLLRRQTHSRPSSE
                     ARALSSLEAAARHLSRLAGDFQTLSRIERGVFELQCGRCDLSHLAQQVVEDHQALAGN
                     HRLILSTPREPVLGDWDGDRLYQVLDNLVGNAVKYSPNGGEVRVALDQQDRLVTVSVT
                     DRGIGIRQTDVEHLFEPYNRLGSPGLSDGQGLGLYIAKTIVEAHGGRLQVASQPGQGS
                     TFRVVRPTTPSQEPPYSKNNEPFPLHSQRR"
     gene            complement(4317..5612)
                     /locus_tag="VHS06_10410"
     CDS             complement(4317..5612)
                     /locus_tag="VHS06_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HEX2988568.1"
                     /translation="MEHDNRQMWADLGIDLVQHDVLLSALPAVFRETHLSQANRPKGM
                     GFFDFVVGDIHGIRVRELREHAKAGGKVVATYCVFVPEEITVAAGAINVGLCAGAQFS
                     VPIAEGVLPRNTCPLIKSSFGFKLGRTCPYVQSSHLVVGETTCDGKKKMFELLNEHQP
                     VYVMEVPNTKTERGRTLWRGEVAAFRDVIEELTGTRITPEALSGAIQLVNARRLALQR
                     LYRTRQADPVPISGKDALVAPQVSFYDDPARDAQMVNALCDELEQRVARNEGVFPAGT
                     PRILVSGSPMAIPNWKLHHILETSGAAVVCEESCTGTRAFSDLTGETGTSLDEQLDDI
                     ADRYMKINCACFTPNDERVEDIIRLAREYRADGVVHYNLQFCHTYAHEAAKVQAALAK
                     EGIPLLRVETDYSDADTAQLRTRVEAFLEMLRPVGASKH"
     gene            6648..6917
                     /locus_tag="VHS06_10415"
     CDS             6648..6917
                     /locus_tag="VHS06_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988569.1"
                     /translation="MSKRLVVLAGVLLLLTWLATACSGTSSASTSAANASSQGGPPRI
                     VVDNAVLDYGKVHYNQWVKPDFRIRNEGGSNLVLQKPTVKTVEGC"
     gene            7272..8642
                     /locus_tag="VHS06_10420"
     CDS             7272..8642
                     /locus_tag="VHS06_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2988570.1"
                     /translation="MEQRPGHGAGQWALAFLRSRWYPGPFQWLTVALLGLLLAFGFWG
                     PDRGGRNLATVITWTLWWPLLPISLVLLGRVWCAICPLAAIASRVQRLFPPGRQRVPG
                     VISRYGVWIMAAAFASLTWVDRVWGITKSPRATGLVLLLLLAGAVAISLAYQRRVWCR
                     YLCPLGALSGLYAMAAPLALRPRQHQCHACSGKECYRGNSRAQGCPLWEFPGNLETNR
                     NCNLCAQCVKACTGKGADGRPSVAMELRLRAPGAELGQVRRPLVGEAALALLMVALVY
                     IQTIDMTSVWPQYMKLVLESTPLDYGLAFTVTLAISLFATMGSYWLAAVLGRRPGEST
                     SRNFAAYGYAYLPLALAGHLGHNASHLLYEGPQAVQTTLDELRIPLALVPPMDESAAM
                     GGINWPALAILGLGIVGAVLTVRRVARNQDQPWRQTWPHLVLLGFWSLAYLVVFLLPM
                     NPRHSH"
BASE COUNT         1723 a         2763 c         2675 g         1719 t
ORIGIN      
        1 gccagagaga gcagctcctc caccaggcgg ctcatacgat tagcctcgct ggcagccgcg
       61 gctgccagct cgtgcctgtc tgcagggggc aaggcctccc cctgcttttc caacaactcc
      121 agattggctc gcaccgctgt cagaggggta cggagctcgt gggaggcatc ggcgatgaaa
      181 cgctgctggg tgttgtacgc gtcttctaag ctggccagca tttcgttgaa ggtgaggccc
      241 agttgaccca actcatctcg ggaattcgtg tcctccagcc ggcgagagaa gcctttggag
      301 agggcgatgg ctcgggcagt gcgggtcata gtggcaatgg gccgcaaggc tgagccggct
      361 atcgcccatc ctatggcgcc ggccagcaac agagagatgc ctccggctag aagtagccac
      421 gagcggagct ggtagagggt ttcaaaggca tcgctgtaag tgcgtgccac cagcacaatg
      481 cccactagct taccttccgc cccgagaggc tgagcataca cccgcacctg ctggccgctc
      541 acatccactg tgtacagagc gctgtttcct cgcctgaccg cgaccaaatc ctgggccccg
      601 atgggcagct gctggtcgcc gaggttgtcg gagcgggcca ccacaaagcc atcaagctgc
      661 attacctgta cgtagatgcc cggggattca aaggcatcga tagggggcag agctacctgt
      721 cccaaagacg gcgaggaggg agaggaagcc cctgtctcta cccggagggt gctagctatg
      781 tgctgggctc ggctgatgat gctgtcatcg ctctcattga tcaggtgata ctccatcaac
      841 atatagagca gcacgctgaa caccaccagg gtgatgccca ataccgctgt gtaccaaagg
      901 gccagacgca gacgaatgga caatgctatc tactcccggt ccctgagcac atatcccgct
      961 cccctgaccg tctggatgag acgaggctca cccttctcct ccagcttgct gcgcaggtag
     1021 cgcacatata cctccaagat gttgtagtcc ccggagaagt cgtatcccca tactcgatgc
     1081 ataatggagt cccgagtgag gaccttctcg ggatgctgca ggaacaaatg cagcagctcg
     1141 tactcagtgg tagtgaagga aatctccctt ccgcctcgtc tggcgacccg ggaccctgtg
     1201 tccagggcca agtccttgta gcgcaggaca ctctcctctt caggctccct ccgccgcagc
     1261 agggcccgca ctctggccaa cagcacctcg aagacaaagg gctttaccac gtagtcgtct
     1321 gccccggttt cgaatcccgt cacttgatcg gccggggcgt ccttagctgt gagcaacagg
     1381 atgggcaatt tcccgtccac cgctcgcagg cgccgggtaa cctccaggcc atccataccg
     1441 ggcatcatga tgtccagaat taccaggtcc ggcgggtttc cccgggccag atccagagct
     1501 tctctgccgc tgccggccac caccacctcg tagccttcat agcccaggcc acgcttgagc
     1561 actgaggtca cccccgggtc atcatcgaca accagaatcc gctcggccat attaggcgtc
     1621 tcctcccctg gacctcggct gattatgggt agcgccagcc ctacttgtcc ctcggccttt
     1681 cgctgggcag cactagaaca acccccaaac cactgccata gtaccactta tgttgagtaa
     1741 tgggcaagaa ccggccaccg ggcactgaga ctgggtattg tgggtattga caagggcctg
     1801 ccatcccccg tgggggatgg caggccgaga agagaagatg gactctctac aggtgccagg
     1861 tactggtgac gctggtaagg cgcggccccg ggcctgtcag ctattcggcc ggcccactag
     1921 ccacggcggc gggcaacgct ttctgtcgtg cccataggtg gtaggccaag gcccctgctc
     1981 cggcgatgga agccagggcc accacctgag cttcctgcag ccccaacaag aacaacgcct
     2041 cctggcgcca gaagctaatc acaaacttcc caaacgagta caggcccagg taagcgagga
     2101 aaaggcctcc ctccagcttg accttacccc tggcccacca tagcaggccg aacaccagga
     2161 ggtcccagag catctcgtaa acctgggtag gctggtaggc atcgcccagg ctgggagcca
     2221 tggccccggg attggtgtag accaagcccc agggcaggct ggtaggagcg cccacggcat
     2281 ccccgtttac tatgcagcca atacggccga tggcctgacc caggatcatc cctggggcta
     2341 cggcatcggc gatcttgcct agaggcagcc cccgtctcgc tatgtacact acggccacca
     2401 ccgtgccccc caggatgccc cccaggatgg ccaggcctcc ctgctggatg gcgatgatgg
     2461 agccaggatt agccgagtag tacccccagt tgtcgattac atgcaggagc ctggcccctg
     2521 ccaaaccgcc cagcacggcc cacaagccca tgttgtagat ctcatcagta tctagccccc
     2581 ggcgcctggc ctcccgagcg gcgatagtga aggcggccag gattcccagc atgatgccca
     2641 cggcatacca ccgcaaggcg aagtggccca caccgatgat caccggatct atgtctacga
     2701 ctatgggccc catattgcca cctgtccttt accccgtttc tgccggtgct tccaggacgc
     2761 tcactgctct gcccgcgccg tccggctaca tcgtatcatc cgggcctgaa ttcatgctga
     2821 atctgaactg agcggcagct taggaaaagc ctgccaacga tggcggcact gcccgcgctg
     2881 cgctccgcca gtctgccaaa ggcataggca acagtctcag ggaagtctca ggccggactc
     2941 agggccatct catcatcacg gtctatactc catccagaag gcaggctgac cgattcgggg
     3001 ccaggtccct gaaagggctg cgaatgtggg tgtagtgagc aaaggtcctc tgggtatcgc
     3061 cgcaaacgag ttccagaggg ctgcgaaacc catgggctca agtactagaa ggggtagtgc
     3121 tattggcaca gatcactctc aaggcaggtg gaccaaaggc gaggcacgtt atcgctatcg
     3181 cttcggccac cctgctcttg ctgctgccta tcgggctgcg cgtcgccttc cctcaggaac
     3241 atatagccct tctgtatgcc ctgcccattt tggtggctgg ccagtccctg gccacgcccc
     3301 tggccgtact agtcgccctg ctggctctgg ccggccattc tgtggactct cttatcgatg
     3361 gctcatcggt atggacctgg cttatccagg gcaccatcct gctgatcgtg gcctacctgt
     3421 cggttcggac tcgtcaagag cagacagagt tggctcgttt ggctggcgaa ctgcaacatg
     3481 tgcatattca gcaggagcga tttgttagct cactggctca cgacatccgg ggtgggatca
     3541 ctgtcatcct cggctacgca caattgttga gaaggcagac acactcgcgg ccgtcatccg
     3601 aggccagggc attgtctagt ctcgaggcgg cagcgcgaca cttgagccgg ctggccgggg
     3661 acttccaaac cctgtcgcgc atcgaacggg gtgtattcga gctacagtgc ggccggtgcg
     3721 acttatcgca cctggctcaa caggtagtgg aagaccacca ggcgctggct ggcaatcacc
     3781 gcctgatcct gtcgacgccc cgggagccgg tactcgggga ctgggatggc gaccgtttat
     3841 accaggtact ggataatctg gtgggtaacg ccgtcaagta ttctccgaat gggggtgagg
     3901 tacgtgtggc cctggaccaa caggatcgac tggtcacagt atccgttacc gatcgaggga
     3961 taggcatccg ccagaccgac gtggagcacc tcttcgagcc ctacaaccgc ctgggatcgc
     4021 ctgggcttag cgacggtcag ggtctggggc tatacatcgc caagaccatt gtcgaggccc
     4081 acggaggccg gctccaagtg gcgagccagc caggccaggg cagcaccttt cgtgtagtgc
     4141 ggcccacgac gcccagccag gagcctccct actctaagaa caacgaaccc tttcccctcc
     4201 attcacagcg ccgctaggcg gccaagcaca ggcctcggcc gcccagagca cgcctccccg
     4261 gcggccgttc atcgaacggc cggagaacgg cacctagacc atatctgtgg cgcctatcag
     4321 tgctttgatg cccccactgg ccgaagcatc tccaagaagg cttccaccct cgtgcgcagc
     4381 tgtgccgtat cagcgtcgct gtagtcagtc tcaacgcgta gtaggggaat accctccttg
     4441 gccaaggctg cctgtacctt agccgcttcg tgggcgtagg tgtggcagaa ctgcaggttg
     4501 tagtgcacca ccccgtcggc ccgatattcc cgagccaggc ggatgatgtc ctccacccgc
     4561 tcgtcgttgg gcgtgaagca agcacagttg atcttcatgt accggtctgc gatgtcatcc
     4621 agctgctcgt ccaggctcgt cccggtttcc cctgtcaggt cgctgaaggc ccgcgtgccg
     4681 gtacatgact cctcgcacac taccgcagcc ccagaggtct ccaggatgtg atgcagcttc
     4741 cagttaggga tagccatcgg gcttcccgaa accaggatgc gaggcgtccc ggccgggaac
     4801 actccctcat tccgggccac ccgttgctcc aattcgtcac aaagggcgtt gaccatctgg
     4861 gcatcgcggg cagggtcgtc gtagaaggaa acctgtggtg ccaccaaagc atccttgcca
     4921 ctgatgggga ccgggtcggc ctggcgagta cggtacaagc gctgcagagc caggcggcgg
     4981 gcgttaacga gctgtatggc cccggagagg gcctcagggg tgatcctggt gccagtcaac
     5041 tcctcgatga cgtccctgaa ggcagccact tcaccccgcc acagggtgcg gccccgttcc
     5101 gtctttgtgt taggcacctc catcacatag accggctggt gctcattcaa cagttcgaac
     5161 atcttcttct tgccgtcaca ggtggtttcg cccaccacga gatggcttga ttgcacgtat
     5221 gggcaggtgc ggcctagctt gaagccaaag gaggatttga tgagaggaca ggtgttgcga
     5281 ggcagcactc cctccgcaat tggcaccgag aactgcgccc ccgcgcagag gccgacattg
     5341 atagctccgg ccgccactgt gatctcttcg gggacgaaaa cgcagtaggt agcgactacc
     5401 ttaccgcccg ccttggcatg ctccctcagc tccctaactc taataccgtg aatgtctccc
     5461 actacgaagt cgaagaagcc catgcctttg ggccggttgg cctgggaaag gtgggtttcc
     5521 cggaaaactg ctggcagggc actgagcagg acgtcgtgct gaacgaggtc tatgccaagg
     5581 tcggcccaca tctgccggtt gtcgtgttcc atatctagtg actgctcctc ttctcttcat
     5641 ggtccagttg ggatggccaa tacacaacaa aaaaacgatg gccaagtgct gaaggcacca
     5701 tgccatcgcc tctctgccgt ttggcagaag cctagagtcg cagattcctg gggagcgcga
     5761 gccggctgag tagggggtgg aggtccttcg agcaggtgac cggtgcacaa cttgcaccgc
     5821 aatgccggtc ggctcaggtt caggtgaagg acgtgagggg attccaaatc accgctcttc
     5881 acctcctccc ctaaagactg ctgagagtgt aacacagcta ggacgtcccg ggccagagag
     5941 ggaggtcatc actcaatcag cgtgcggttc tccggatgac cttatagggg cgcggcataa
     6001 cgcgcggaac tacgacccgc taggccttcg atggcgcttt gacccgtcgc caaggccaaa
     6061 agcgtgacct cgtgtcggca gattgttggt tctccagtgc aaaccgggca gtctcctctc
     6121 gttcttcctt ccgagccagg cgtacgtaca tcaccaatag gacgacaaac atcggcagcg
     6181 ccagcagcag cgagcgcatc tccctttggc tatcaaatct gctcccacga tccttttggt
     6241 cacggattca cgggccaaac tctcaggcgt atctcagttc atgttcagta cccgctcaga
     6301 cgactatgag aagattcccc cggacggctc cttagcgttc cgccaagaac attgatcagg
     6361 agcagccact gcaaagaatg aaccggaacg aggagaaagc acgacacgaa catgcacgtg
     6421 acggtattgt gatgactgtc gcaccgctgc ttggcccact tctcaggcga ttctcagcat
     6481 tgtctcagcc ctcgttaagt ccggggcggt atcattactt gacgtcctgg tcccgaagcg
     6541 tagctcgcat agggaggccg ggttggtggc tgtgcggaag gcggctccac caggacgtcc
     6601 caaagagatg gaccagcgag atgggccgag gagaggaagc gaaggggatg agcaagcgac
     6661 tagtggtact ggcgggggta ctgcttctgc tgacctggct ggcgacggcc tgctccggaa
     6721 ctagcagcgc tagcactagc gctgccaatg ctagcagcca gggtgggccg ccccgtattg
     6781 tcgtggacaa cgcagtgctc gactacggca aggtgcacta caaccaatgg gtgaagcctg
     6841 atttcaggat caggaatgaa gggggcagca acctggtgct gcagaaaccc acggttaaga
     6901 ccgtggaggg ctgttgacca cccgatgccg tcgtgggttc cacgacacta cgcccgggtc
     6961 aatctaccag cttaaatgct ccgttcacca tgcacgccgg gatggatggt cctcacgatt
     7021 tcgaggtaca cgttcgtagc aatgacccat tgaagcctga cctggtggtc catgtgaagg
     7081 ccgactacat tccctaggag gcgtcgggga gcgttggggg ccctgtttgg gatcccccca
     7141 agcgccggct ccgatgtggg ctggggcacc caggcctgga ggcacccgcg gatgggcaag
     7201 cttagatgcc gaaaatggtg gtagatactc aggagacgcc agcgccgcca cccatgaatg
     7261 aagcgcctcg agtggagcag cgaccggggc acggggcggg gcaatgggca ctggcgtttc
     7321 tcagaagtcg ctggtatccc ggtccgttcc agtggcttac ggtggccctt ctggggctgc
     7381 tgctggcctt cggcttctgg ggcccggacc gcggtggccg caacctggct accgtgatca
     7441 cctggacgtt atggtggccc ctgctcccca tctcactcgt cctattgggc cgggtgtggt
     7501 gcgccatctg cccgctggcg gcgatagcca gccgggtgca gcggctcttc cccccgggaa
     7561 ggcagagagt gccgggggtc ataagccgct acggcgtctg gattatggcg gcggcctttg
     7621 cctcccttac ctgggtagac cgcgtctggg gcattaccaa gtcaccccgg gccaccggct
     7681 tagtgctctt gctgctgttg gctggagcgg tagcgattag cctagcctac cagcggcggg
     7741 tttggtgccg ctacctgtgt cccctagggg ccttgagcgg gctttacgcc atggcagctc
     7801 ctttggccct gcgaccccga cagcaccagt gccacgcgtg cagtggcaag gaatgttacc
     7861 ggggcaactc tagggcccag ggctgcccgc tgtgggagtt cccgggcaat ttggagacaa
     7921 accgcaactg taacctctgt gctcagtgcg tgaaggcctg tacggggaag ggcgccgacg
     7981 gccggccgtc ggtggcgatg gagctccgtc tgcgagctcc aggcgctgag ttggggcagg
     8041 tccgtcgccc gctagtgggg gaggcggcac tggctctgct gatggtggcc ctggtctaca
     8101 tacagaccat cgatatgacc tccgtatggc cccagtacat gaaactcgtg ctggagtcga
     8161 cgcctttgga ctacgggttg gccttcaccg tcaccctcgc catctccctg tttgctacta
     8221 tgggctcgta ctggctggcc gccgtgctgg gccgcaggcc aggggaatcg accagccgca
     8281 acttcgctgc ctatggctac gcgtatctcc cgctcgccct ggctggacat ctggggcata
     8341 acgcttcgca cctgctgtac gaaggcccgc aggcggtgca gacgaccctg gatgagctac
     8401 gcatccccct ggccttggtt cctcctatgg acgaaagcgc cgctatgggc ggtatcaact
     8461 ggccggcgct ggccatcctc ggcctgggta tcgtgggagc tgtcttaacc gtgcggcggg
     8521 tggcccgcaa ccaagaccag ccctggcgcc agacgtggcc acacctggtg ctgctagggt
     8581 tttggtccct ggcatacctg gtagtgtttc tgctgcccat gaacccgcgc cacagccatt
     8641 agctggcgcg gcgagtagct cgacggcatc aaatgcacac tacggcggtg ggggcccacg
     8701 gacttgggcc cggtgaaata tcggagagtt tcgctccacc cgtcgggaga acagcagcag
     8761 cggtggttca gtctccgtca gcgagtttga ggtgagcaga cagtgaccaa atgggtattg
     8821 gcggcgctta ttgccgggct cggaaggaag aacgagagga gactgcccgg tttgcactgg
//
LOCUS       DATALS010000468         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_351123, whole genome shotgun sequence.
ACCESSION   DATALS010000468 DATALS010000000
VERSION     DATALS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351123"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..938
                     /locus_tag="VHS06_10425"
     CDS             126..938
                     /locus_tag="VHS06_10425"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2988571.1"
                     /translation="MSADSRSGDVVETGRWQDATLAGFHQAALSLGDGRAPLYHQLKE
                     LLRDLIESGQWARGERIPPERELCATFNVSRATTTQALNDLERMGLVERRQGKGTFVA
                     RPKLVQDLLDFRTLTRGTLASDLTPTMKILSMEVVDPVPRLAQLLEVSMGEKLIRIER
                     LRMVNDEPMMMDVCSLPYAMCPDLLHDNLEENLLYDLLSQKYGIKMVEQEKWVEPVML
                     DDFEAGLLRTKKGALGLLVERVAYCEGRRPMEFRKMLVPGHRCKYVVEVSRP"
     gene            1037..>1968
                     /locus_tag="VHS06_10430"
     CDS             1037..>1968
                     /locus_tag="VHS06_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988572.1"
                     /translation="MDKGLPASLHTRAVSVLEATFGLVSNNQWLLHNPEFFSEYKMQC
                     LLTDVHRVDDIPFDSNLDAYLYLIDLPDVPPSVRVGDREVWCAGPFSRLDAAMADRRY
                     SVFGNLGLFFKYCMATMERRHGIYSFHASAMYVPERNELIAVIGGAGAGKTVLILEGL
                     SRGYQIFTVEMLHFAFRDEGLVFFKGALMDNIRVGNLVYDFPGAAERLGIQIPEVKDV
                     WGTKIAVDFQPMAPSSDVIVNPKLTLLFPRIEAGRDPAIISDITSQSKLKWMLFNNAT
                     EKIGGTNLFYETVVVDTMDTPDLMQKRLKAMERLV"
BASE COUNT          396 a          583 c          593 g          396 t
ORIGIN      
        1 ctcctgcacg acttgtcatg ataaccggcc caactgtata atagcttcag cctcttagtt
       61 gaggctggcc ctccagccgt gatggggtcc taccgcaccc gtttccctcg ggagggacaa
      121 ggacgatgag cgccgacagc aggtccggcg acgtggtaga aaccggacgt tggcaggacg
      181 cgaccctggc cggttttcac caggcagctc tctcactggg tgacggccga gcccccctct
      241 accatcagtt gaaggagctg ctgagggacc tcatcgagtc gggacagtgg gcgaggggag
      301 agcgaatccc gccggagcgc gagctgtgtg ccactttcaa cgtcagccgt gctacgacca
      361 cgcaggccct gaatgatctg gaacggatgg gtttggtgga gcgacgacag ggcaagggca
      421 ccttcgtcgc gcgtccaaag ctcgttcagg acctcctgga cttccgtacc ctcacacgcg
      481 gcacactcgc cagtgacctt acccctacta tgaagatcct ttccatggag gtggtagacc
      541 ctgttccacg gttggcgcaa ctgctggagg tttccatggg ggagaagctg atccgcatcg
      601 aacggcttcg gatggtaaac gacgagccga tgatgatgga tgtgtgttcg ctcccatacg
      661 ccatgtgtcc ggaccttctc cacgacaatc tggaggagaa cctgctttac gaccttctct
      721 cgcagaagta tgggatcaag atggtcgaac aggagaagtg ggtggagccg gtgatgctgg
      781 acgacttcga ggccggactg cttcgcacca agaaaggggc tctgggactg ctcgtcgagc
      841 gggttgctta ttgcgagggc aggaggccga tggagttcag aaagatgctc gtgccgggac
      901 acaggtgcaa atacgttgtg gaggtgagcc ggccctaact attttcctta catatcagtc
      961 gatagggtga tctgtcggcc taacaacgcc actaatacgc cgctacggta gcggaaaacg
     1021 gaggcttcct ttggcaatgg acaaaggtct tccggcctcg cttcacactc gcgccgtttc
     1081 ggttctcgag gcgactttcg gactcgtctc caacaatcag tggcttctgc acaacccgga
     1141 gttcttcagc gagtacaaga tgcagtgcct cctgacggac gtccaccggg tcgacgacat
     1201 cccgtttgat tccaacctcg acgcatacct ctatctgatc gatttacccg acgttccccc
     1261 gagcgttcgc gtcggcgacc gcgaggtgtg gtgcgctggc cccttcagcc gtctcgatgc
     1321 cgccatggcg gaccgacgct acagtgtctt cggcaacctc ggtctcttct tcaagtactg
     1381 catggccaca atggagcgac gtcacgggat ctacagcttc cacgcctccg cgatgtacgt
     1441 tccggagcgc aacgagctga ttgccgtcat tgggggggcg ggcgcaggca agaccgtgct
     1501 gatcctggag gggctcagtc gcgggtacca gatcttcacg gtggagatgc tccactttgc
     1561 cttccgtgac gagggtctcg tcttcttcaa gggagccctg atggacaaca tcagggtggg
     1621 caacctggtc tacgacttcc ccggggccgc cgagaggctg ggcatccaga tccccgaggt
     1681 gaaggatgtt tggggaacca agatcgccgt ggacttccaa cccatggccc ccagcagcga
     1741 cgtgatcgtg aacccgaagc tgacgctgct cttccctcgc atcgaggcgg ggcgcgaccc
     1801 tgccatcatc agcgacatca caagccagtc gaagctcaag tggatgctgt tcaacaacgc
     1861 cacggagaag atcggcggca ccaacctgtt ctacgagacc gtcgtcgtcg acacgatgga
     1921 tacccctgat ctcatgcaga agcgtctcaa ggcgatggag cgcctggt
//
LOCUS       DATALS010000469         4874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_72135,
            whole genome shotgun sequence.
ACCESSION   DATALS010000469 DATALS010000000
VERSION     DATALS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72135"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VHS06_10435"
     CDS             complement(<1..746)
                     /locus_tag="VHS06_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988573.1"
                     /translation="MHGGGWWSYIRYDEKQDKPNVSFALLRRVGHYARPYTRQIVLML
                     LTIVGIDLLTLIPPLLYRDLIDRALPAQDVGRLNLLAVGMIGIPLLSGLIGVGQRYLS
                     SAIGEGIIFDLRGSVFEHMQKMSLRFFTNTRTGELMARLNNDVVGAQQAVTSTSVNII
                     ANIFALVMTLAVMLSLEWRLTLMSVAVLPFFILPARRVGSMLRTVTRDGMNMNAGMNA
                     MMNETLNVSGALLVKIFGRERDENRRFQERA"
     gene            complement(993..2801)
                     /locus_tag="VHS06_10440"
     CDS             complement(993..2801)
                     /locus_tag="VHS06_10440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX2988574.1"
                     /translation="MTESNVQTALQPEIVWKLDDLYLAPDDPQIDVDLSRAREMADSF
                     SQRYRGKVADLDPAALLGALRELEEIITVGYKPMGYASLAFAAQTQDQSRQALVSRVR
                     EVTTDVSNRLVFFDVELKTMPEERFAPLAEAPELAGYRHHLETLRAFAPHTLSEPEER
                     LFARMRLTGASAWSQLYTEVTSGLRFRVEVDGEVKELNDPATRALRTKPDRGVRERAN
                     DVLYSVYSDNSRVLTYIFNTLFQDHKLDVEARSFSSTMEPTALANELSPEVIERLLSE
                     VEAHYSLAQRYYRTKASLLGLEGDFRYYDLLAPLRKEETRYDFDQAKEMVLNAFGEFH
                     PRMRQISEQFFQNEWIDATPRLGKRGGAFCSGLLPAYHPYVLTNFTGRLDDVFTLAHE
                     LGHGVHFYLARQQTPMNYSPTTPMAEVASVFGEILLSKRLRESEQSPEMRLSILSGMI
                     EDAIATIFRQTMYTRWEQRAHAARGKAVVPAEEYSSLWLEESQRLYGDSVIMGDLDRW
                     GWVTIPHFVQYRFYCYSYAFGHLLNFALYQSYMEKGDQFVPGYLELLSSGGRDRPEKL
                     LEAVQMNPLEPGFWDRGFQVLEGLLREFEQAAAAVS"
     gene            3034..4830
                     /gene="pepF"
                     /locus_tag="VHS06_10445"
     CDS             3034..4830
                     /gene="pepF"
                     /locus_tag="VHS06_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX2988575.1"
                     /translation="MEKLPKRNELPEAYTWNLETIYPDESQWEADFNRVKDLIPRIQA
                     LQGTLGNSAAQLLGCLELRDETSKPLEQLLVYARMRKDEDNANNHYQALSDRAMVLAT
                     QFSSATAFLVPEILAMPEDKLQGFLTAEPKLELYKHYLDELLRQRPHVRSTEVEVVLA
                     QASEVTRTSDNSYGMLTNADMKFPTIKDEQGNDVELSQSRYGQFRESPDRRVRSEAFK
                     AMHNSYQNFRNTLASSLSGSVRSDIFYARARNYPSSLEAALGPNNIPTQVYTNLVETV
                     NKNLSLLQRYMSLRKRLLGLDELHHYDLYVPMVADVEVKMPYDEAAQTVLEALSPLGT
                     EYQGVLKNGLSSRWVDVYENEGKTSGAYSGGSYTTYPFVLMNYQGTLTDVFTLAHELG
                     HSMHSYFTRNTQRYIYGDYTIFLAEVASTLNETLLTAFLLKRTNDRKLRSYLVNQQLE
                     RFRTTLYRQTMFAEFELDIHSRAEAGEGLTPDMLSSLYADLNARYYGKEVMPDPEIAI
                     EWARIPHFYWAFYVYQYATGISAAAALAHQILTEGEPAVQRYIRFLKSGSSNYSIDLL
                     RDAGVDMTSPAPVQQALDSFSEMLDQMEELTA"
BASE COUNT         1073 a         1558 c         1362 g          881 t
ORIGIN      
        1 gcccgctcct ggaaccgccg attctcgtct cgctcccggc cgaagatctt caccagcagc
       61 gcaccgctga cgttcagtgt ctcgttcatc atcgcgttca tgccggcgtt catgttcatg
      121 ccatcgcgag tcacggtgcg cagcatcgac cccactcggc gagcaggaag gatgaagaag
      181 ggcaacacgg cgacggacat caacgtcaaa cgccactcca gggacagcat cactgccaga
      241 gtcatcacca gggcaaagat gttggcgatg atgttcacgc tggtactggt aaccgcctgt
      301 tgggcaccga ccacgtcgtt gttcagacgg gccatcagct cgccggtacg ggtgttggtg
      361 aagaagcgca gcgacatctt ctgcatgtgc tcgaagacgg agccacgcag gtcgaatatg
      421 atcccctcgc cgatcgccga gctgaggtaa cgctggccga ccccgatcaa tccgctgaga
      481 agcggaatgc ctatcatccc cacggcgagc aggttgagcc tccccacatc ctgggcagga
      541 agcgcccggt ctatgaggtc cctgtacaga agcggtggga tcagggttag caggtcgatc
      601 ccaacgatcg taagcagcat gagaacgatc tgccgagtgt aaggccgcgc ataatgcccg
      661 acacggcgca acagggcaaa gctcacgttg ggcttatcct gcttctcatc gtaccggatg
      721 taggaccacc agccgccacc gtgcatgtta cgtcgagtgt cactcctgaa ggattagggg
      781 ttgaaagccg caccccaggc acccaccaaa acaccgcgag tgtccggggc tgtatcttta
      841 tctctcttgt cgaaatgcag gattcacgcc gaagaaccga gagcggcgtc ctgcagaacg
      901 ccactcctgc ccctatcata ccaccggaga atgccggagc accgtatcgt ctcgtccagc
      961 aagctcaacc aggaggcgag tgacgattcg ccctacgaga cggctgcggc tgcctgctcg
     1021 aactccctca gcagcccctc cagcacctgg aacccccgat cccagaagcc cggctccagc
     1081 gggttcatct gcaccgcctc cagcaacttc tccggccggt cccgccctcc gctcgacagc
     1141 aactcgaggt atccgggcac gaactgatcg cccttctcca tgtagctctg gtagagcgca
     1201 aagttcagca ggtgaccgaa ggcgtagctg tagcagtaaa agcggtattg gacgaagtgg
     1261 ggtatcgtca cccaccccca gcgatccagg tcgcccataa tcacggagtc gccgtacaga
     1321 cgctggcttt cctccagcca gagcgaggag tactcttccg ccggcactac tgccttgccc
     1381 ctggcggcgt gggcacgctg ctcccaccgg gtatacatcg tctggcgaaa gatggtggcg
     1441 atggcatcct ctatcatccc gctcagaatg ctcaatcgca tctcgggaga ctgctcggac
     1501 tcacgaagcc gcttggagag caggatctct ccgaaaacgc tggcgacctc ggccatagga
     1561 gtagtagggc tatagttcat cggcgtctgt tgccgcgcaa ggtagaagtg cacgccatgc
     1621 cccaactcgt gagccagggt gaagacgtcg tccaaccgac cggtgaagtt ggtgagcaca
     1681 tagggatggt aggcaggcag cagcccagag cagaaggctc caccgcgctt tccgagtcta
     1741 ggtgtggcgt cgatccactc gttctggaag aactgctcgg aaatctgtcg catccgaggg
     1801 tggaactccc cgaaagcatt cagcaccatc tccttggcct gatcgaaatc gtaccgagtc
     1861 tcctccttgc gcagcggcgc gagcaggtcg taatagcgaa aatccccttc cagtcccagg
     1921 agcgacgcct tggtccggta gtaacgctgc gccagggagt agtgcgcctc cacctcagag
     1981 agcaaccgct cgatcacctc cggcgagagc tcattggcaa gcgcggttgg ctccatcgtc
     2041 gagctgaagg aacgagcctc cacgtccagc ttgtgatcct ggaagagggt gttgaatatg
     2101 taggtaagca cgcgactgtt gtcggagtag acggaataga gcacatcgtt tgcccgctct
     2161 cgcaccccac ggtccggttt cgtgcgcaat gcacgggttg ccgggtcgtt cagctccttc
     2221 acctccccgt ccacttccac ccgaaagcgc aacccgctgg tcacctcggt gtatagctgg
     2281 ctccacgcgg aggcgcccgt gagccgcata cgggcaaaca gccgctcctc gggctccgag
     2341 agggtgtggg gagcgaacgc ccgaagggtt tccagatggt ggcgatagcc cgccagctcg
     2401 ggtgcttccg caaggggggc aaaacgctcc tccggcatag tcttcaactc gacatcgaag
     2461 aagacaagcc tgttagatac gtcggtggtt acctcccgaa ccctggacac caatgcctgc
     2521 cgactctggt cctgcgtctg cgcagcaaaa gccagagagg catagcccat gggcttgtaa
     2581 ccgacggtaa tgatctcctc caactcccgc agggctccaa gcagcgccgc agggtcgagg
     2641 tccgccacct ttccgcgata ccgctgtgaa aagctgtccg ccatctccct ggctcgactc
     2701 agatccacgt cgatctgcgg gtcgtccgga gccaggtaga ggtcatccag cttccagact
     2761 atctccggtt gaagggctgt ctgcacgttg ctctcagtca cacaaacctc ccgatcacgc
     2821 actgcctcgg ccacacgatc tccgaaacgg attcagacca ccgttcgtta agaaagacgc
     2881 atacttcgga ccgaatcatg caccaccatt ctgaaggcga agtataagct atcgtcgcgc
     2941 gtccgttgta aggcccacca ctggccgata ggggcctgct tctgctacga tggcagcagg
     3001 gcaagaagaa gtgcaagcag gagaccggga cgaatggaga aactgcctaa gagaaacgaa
     3061 ctaccagagg cctacacctg gaatcttgaa accatctacc ctgacgaatc gcaatgggag
     3121 gccgatttca acagggtcaa ggatctcatc cctcgcattc aggcgctaca gggaaccctg
     3181 ggcaactcag cagcccaact gctgggctgt ctggaactcc gcgacgagac ctccaaaccg
     3241 ctggagcaac tactcgtcta cgcccgaatg cggaaggacg aggataacgc caacaaccat
     3301 tatcaggccc tctctgaccg cgccatggta ctggcgacgc agttctcgag tgccacggcc
     3361 ttccttgtcc ccgagatcct cgcaatgccc gaagacaagc tgcaggggtt ccttaccgcc
     3421 gagccgaagc tggagctata caagcactac ctcgatgaac tgctgagaca acgtccccac
     3481 gttcgatcca ccgaggtgga ggtcgtcctg gcccaggcgt cggaggtgac acgcacctcc
     3541 gacaacagct acgggatgct gaccaacgcc gatatgaagt tccccaccat caaggacgag
     3601 caggggaacg acgtagagtt gagccagtcc cgctacgggc agttcaggga gagtccggat
     3661 cgacgggtgc ggagcgaggc attcaaggca atgcacaaca gctaccagaa tttccggaac
     3721 accctggcat cttctctcag tgggagcgtc cgctcggaca tattctacgc ccgagcgcgc
     3781 aactacccgt cctccctcga ggctgccctg ggacccaata acatcccgac ccaggtgtac
     3841 accaatcttg tggagacggt gaacaagaac ctctcgcttc tgcagcgcta tatgagtctg
     3901 cgaaagcggc ttctagggct ggatgaactg catcactacg acctgtacgt ccccatggtg
     3961 gcggatgtgg aggtcaagat gccctacgac gaggcggcgc agacagtact ggaggcactc
     4021 tctcccctag gaacagagta ccagggtgtg ctgaagaacg gtctctcatc ccgatgggtg
     4081 gacgtgtacg agaacgaagg caagaccagc ggtgcgtact caggcggctc ctacacgaca
     4141 taccccttcg tcctgatgaa ctaccaggga accctgaccg acgtgttcac gctggcccac
     4201 gaacttggac actccatgca ctcctacttc accaggaaca cccagcgtta catctacgga
     4261 gactacacca tcttcctggc agaggtagcc tccacactga acgagacgct gctaaccgcc
     4321 ttcctgctga aacgaacgaa cgatcgaaag ctccgctcct acctggtcaa ccagcagttg
     4381 gagcgattcc gcaccaccct ctatcggcag actatgttcg ctgagttcga actggacatc
     4441 cactcgcgag cagaggccgg tgaggggctg acgccggaca tgctctcctc cctgtacgcc
     4501 gacctgaacg cccgctacta cggcaaggaa gtgatgcctg atccggagat cgccatcgag
     4561 tgggcacgga tcccccactt ctactgggcc ttctacgtct accagtacgc cacggggatc
     4621 tccgccgcgg cggcactggc ccatcagatc ctgaccgagg gcgagccggc cgtgcagcgc
     4681 tacatccgat tcctgaagag tggctcctcc aactactcca tagacctgct ccgcgacgcg
     4741 ggcgtcgata tgacctctcc cgcgccggta cagcaagcgc tggacagctt cagcgaaatg
     4801 ctggaccaga tggaagaact gactgcatag gcttccgaca ttagtagggc gggtcgcccc
     4861 tgacccgccc gtct
//
LOCUS       DATALS010000470         8372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_443240, whole genome shotgun sequence.
ACCESSION   DATALS010000470 DATALS010000000
VERSION     DATALS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443240"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..779
                     /locus_tag="VHS06_10450"
     CDS             87..779
                     /locus_tag="VHS06_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988576.1"
                     /translation="MPTHYRGDEKEVLAGTLIQERELWLNISTGSQASLLTPKPEAGE
                     FRVRPAIDGRYYRTITHIPAGRSLSLLVDLLSELAKADGLELRDPWAYLAAAAESIEE
                     TDLEVDLAFFVGPCGDRGRIGNIREGNLTAGHVFRAALENMAGNYYSCALRLSPEKDW
                     SKLALSGGLPQKLPQLRHLISRRFGMEYRLPASEEDTLLGLLALALVRTGDASSMAEA
                     SGMLGEKTSGSR"
     gene            complement(846..1733)
                     /locus_tag="VHS06_10455"
     CDS             complement(846..1733)
                     /locus_tag="VHS06_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HEX2988577.1"
                     /translation="MSDDNRSRTILVVGATGQQGGAVARKLLEGGWRVRVLTRDADSD
                     KARALASAGAEVVEGDLNSRESLDRAVAGAYGVFSVVNFWLPGIGFEGEVRQGKTIAD
                     AAKSANARHFVYSSVGGAERNTGIPHFESKWQVEQHIRELGLPATIFRPVAFMENYYF
                     TRDAILNGTLSQYGVRPSKTVQLIAVEDIAAFVALAFERPEEFIGKELEIAGEELTMP
                     QQAEIIGRVIGRPVLYVQTLPEGDWNRGEEARRMTRWFDEEGYKADIPALRRLHPEMM
                     NLETWLRRMGWEGAARLAA"
     gene            complement(1778..2110)
                     /locus_tag="VHS06_10460"
     CDS             complement(1778..2110)
                     /locus_tag="VHS06_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HEX2988578.1"
                     /translation="MTVNGAKQRLESAVATWPGTTAHPHRFGGTEFRLGRREIGHIHG
                     DYLVDIPFPRKIRDELIAAGRCEPHHVLPQSGWVSAYIRSEPDVENAIDLLRISFDLA
                     TKHHSQVA"
     gene            complement(2127..2603)
                     /locus_tag="VHS06_10465"
     CDS             complement(2127..2603)
                     /locus_tag="VHS06_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX2988579.1"
                     /translation="MPTHYRGDEKEVLALDAFIKLTRAMESVGSRLFACGCLGDLTTS
                     QFSVLEALYHLGPMNQTEVSSKLLKSTSNMVTVIDNLENRGLVRRQRDQRDRRSVVVI
                     LTDSGSEVIARVLPLHVEAIVKEMSVLTPGEQRELGRLCRKLGKRETAPESPDSSC"
     gene            complement(2728..3945)
                     /gene="glgA"
                     /locus_tag="VHS06_10470"
     CDS             complement(2728..3945)
                     /gene="glgA"
                     /locus_tag="VHS06_10470"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX2988580.1"
                     /translation="MAEIQKVALFTNEYPPNVYGGAGVHVEYLSRELARHTRVEVRCF
                     GNQDSADGNLTVKGYPAWPGAKENTDPRFAGAMDAFGRSLAMAKDNLDAQVVHCHTWY
                     TDMAGFIAKKLWDTPLVLTIHSLEPRRPWKVEQLGNAYYLSTWMEHTSIEDADAIIAV
                     SKEVKEDVLRFFNVSPHRVHVIHNGIDLEEFRYDPSTDALVERGIDPNRPYVLFVGRI
                     TRQKGIMHLVRAIPEIDPSLQVVLCANSPDTEEIGQEMTEWAGEVRAKREGVFWFLEK
                     VPLPDLIQFYSHAAVFCCPSVYEPFGLINLEAMACETAVVASNVGGIPEVVVPEETGL
                     LVDPRLEEDDFEPVSPARYSRELAAAINRIGLDPALQERMGKAGRQRAEEHFSWSAIA
                     RKTLSLYGSLVSS"
     gene            complement(3945..5327)
                     /locus_tag="VHS06_10475"
     CDS             complement(3945..5327)
                     /locus_tag="VHS06_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX2988581.1"
                     /translation="MKVNPGIFKAYDIRGVYPTDLDEETAFGIGRAFVTFLEAKEVLV
                     GRDMRISGPQLFEAVTRGIMKQGADVIDIGMVSTDQYYFACSQLKVPGIMVTASHNPK
                     AYNGFKMVHQMPYLLSGDQGIQDLRRIVEEDAYAPATREGTMRSMDLSEQFVEAVLSL
                     IDIPAIKPLKVVVDTGNGMVGPVLQRVYSRLPVKLVGMYLDPDGNLPNHGLDPLQPEN
                     RAELQQRVRSEKADVGFAFDGDGDRFFVIDNRGEFVAGDYLTALLGSYLLKKQPGSKI
                     VYDVRASWAVPDLVREAGGVPLMERVGHAFIKRRMADEGVLFAGEVSGHYYFKGFNFA
                     DSGIIPSLLVIEMISTSGQSMADLLHSLEEKYFISGEINSTVEDPTAKMQELAVEYSD
                     GKIEWIDGISVTYPNWHFNVRASNTEPLMRLNLESTTSREDMEELRDEVLGIIREPAE
                     EEEETGDEEF"
     gene            complement(5379..6662)
                     /locus_tag="VHS06_10480"
     CDS             complement(5379..6662)
                     /locus_tag="VHS06_10480"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX2988582.1"
                     /translation="MSVLTLILAGGEGSRLSVLSEKRAKPAVPFAGKYRIIDFALSNA
                     VNSDLYSIAVLTQYRPHSLMHHIGIGEPWDLNRRWPNGVQMWQPYRGRSDQDWYQGTA
                     DALYQNRNFIADIGCDEVLVLSGDHIYKQDYRELLRFHRENRAELTVGVINVRPDEVH
                     RFGIMTVDGSNRIVKFAEKPRQSDSTLASMGVYVFNRETLLQRLEDDAHDHASAHDFG
                     KNIIPNMVDRDRVVAFPFDGYWVDVGTVQAYWEANLALLADKPDLDLFDSTWTIHTRS
                     EERPPAKFGAPGEIENSLVSNGCRVYGTVINSVLSPGVLVEPGAVVRDSVIMTNTAIG
                     PGAVVDRCVIDKDVDVGAGARVGSGEDNRPNDREPANLSSGITVVGKRATIPPDVVIG
                     RNCRIDAGVSHADFREREVPSGATIAPRAALDKAA"
     gene            complement(6728..7741)
                     /gene="fbp"
                     /locus_tag="VHS06_10485"
     CDS             complement(6728..7741)
                     /gene="fbp"
                     /locus_tag="VHS06_10485"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX2988583.1"
                     /translation="MPDVDGLSTIETHILAEQRIHPEATGALTGLLYDLALAGKIIAN
                     EMTHAGLAQILGQTGDVNVQGEEVMKLDVLADQAVRTLVSRGGRVAAMVSEEHAEMIQ
                     VPAGASSARYVLVYDPLDGSSNVNYNVSVGTIFGIFRRTNPGAVATTEECLRVGRDMV
                     AAGYIVYSASTMLVYSAGHGVHGFTLDRGLGEFLASHPDIRIPEEGRYYSVDQGYQKY
                     WGEGARQFTSYLQGFEGGEGMSQRYVGSLVADFHRTLLSGGVFYYPANSKEETKSQGK
                     LRLLYEAAPLSFIAEQAGGYASDGTQSILEIQPAQLHQRTPLFIGDRRLVMKAEEFIK
                     QYG"
     gene            complement(7763..8233)
                     /locus_tag="VHS06_10490"
                     /pseudo
     CDS             complement(7763..8233)
                     /locus_tag="VHS06_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958586.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
     gene            8172..>8372
                     /locus_tag="VHS06_10495"
     CDS             8172..>8372
                     /locus_tag="VHS06_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988584.1"
                     /translation="MPAAITAAIGSYTWLASSPTDNIHLPRNLEGLKPVLILPFLGTL
                     VVGLAMIYVIGTPVQAILQALTT"
BASE COUNT         1479 a         2725 c         2438 g         1730 t
ORIGIN      
        1 gaaatcgcgc cccctttctt cgtggagatg cgtgggcacg tcatatgcga ttggttcgac
       61 atcaaactat ccgacgtgaa caagaagtgc cgacacacta cagaggcgac gagaaggaag
      121 tcctggctgg aacgctgatc caggagcgag aactctggct caatatctcg accggttcgc
      181 aggctagcct tctgacgccg aagccggaag caggggagtt ccgggtccgt ccggccatcg
      241 atggccgtta ttaccggacc atcacccaca tccccgcggg ccggtcgcta tcattgctag
      301 tagatctcct atctgagttg gcgaaagctg atgggctcga acttcgggac ccatgggcct
      361 acctcgccgc ggccgcggag tcgatcgagg agactgacct cgaggtagat ctcgccttct
      421 tcgttgggcc gtgtggggat cggggtagga taggcaacat ccgcgaaggc aacctcaccg
      481 ccggccacgt attcagggct gctctcgaga acatggccgg caactactac agttgcgccc
      541 tgcgcctctc gccggagaag gactggagca agctcgccct ctccggcggc ctaccccaga
      601 aacttcccca actgaggcat ctcatatcca gacggttcgg gatggagtac aggctgcctg
      661 ccagcgagga agacaccctc ctcggtttgc tggcactcgc gcttgttcgc accggcgacg
      721 cgtcctcgat ggcggaagcc tccggcatgc tgggggagaa gacctcaggt tcgcggtaat
      781 tccccgtcca tactggccgg caagctgaac cgccgcgatg ccggccgggt tgggagtcgg
      841 gccgtctacg ctgctaggcg ggctgcgccc tcccagccca tcctgcgcaa ccaggtttcc
      901 aggttcatca tctcgggatg gagtctgcga agggctggga tatccgcttt gtagccttcc
      961 tcatcaaacc agcgggtcat ccttcgtgcc tcttcgccgc ggttccagtc cccttctggc
     1021 agcgtctgca catacagcac cggccggccg attacccttc cgattatctc ggcctgctga
     1081 ggcatggtga gttcctcgcc ggcgatctcc agttccttgc cgatgaactc ctctggtctc
     1141 tcaaaggcga gagctacgaa ggccgcgatg tcctctacgg cgatcagttg cacggtcttg
     1201 gaaggccgga ccccgtactg cgatagggtg ccgttcagga tcgcgtctct cgtgaagtag
     1261 tagttctcca tgaaggcaac cggacgaaag atcgtcgcgg gcagacccaa ctcccggatg
     1321 tgctgttcca cctgccactt gctctcgaag tgagggatac ctgtgttgcg ctccgccccg
     1381 ccaaccgagc tgtagacgaa gtggcgggcg ttggcggact tggccgcgtc cgcgatggtc
     1441 ttgccctgcc gcacctcgcc ctcgaacccg atgccgggca gccagaagtt gactacgctg
     1501 aacacgccat aggcaccggc cacggcgcgg tccagcgact ccctactgtt tagatcgccc
     1561 tccaccactt ccgcgcccgc ggatgccagc gcccgggcct tatcgctgtc ggcatcgcgc
     1621 gtgaggactc ttactcgcca tcccccttcg agcagtttgc gcgctactgc tccgccctgc
     1681 tgaccggtcg cacctacgac cagaatagtt ctgcttcggt tatcgtcaga cacgatgtag
     1741 tctccttgtt ccaggttccg ccagctacca tcggatgcta ggcgacctgc gagtgatgtt
     1801 tcgtcgccag gtcaaacgag atacggagca gatcgatcgc gttttccacg tctggctccg
     1861 accggatgta cgcgcttacc catccacttt gcgggagcac gtgatgaggc tcgcatcgcc
     1921 ctgccgcgat cagctcgtcc ctgatcttcc ttgggaacgg gatgtccacc aggtagtccc
     1981 cgtggatgtg cccaatctct cgccttccga gtcggaactc cgttccgccg aaccggtgag
     2041 gatgtgcagt tgttcccggc caggtggcga ctgcactctc caacctctgc tttgctccgt
     2101 tcactgtcat gttcgtgcct ttgctgctag caactggagt cgggggactc gggggcggtt
     2161 tcccgcttgc ccagtttcct gcacagtctg cccaactcgc gctgctcgcc gggagtcaaa
     2221 acactcatct ctttgacgat cgcttcgacg tgcaggggaa ggacgcgagc gatcacttca
     2281 ctgccggagt cggtcagaat cacgacgaca ctgcgacggt ctcgctgatc cctctggcgc
     2341 ctcaccagcc cccggttctc caggttgtct atcaccgtga ccatgttgct ggtgctcttc
     2401 aagagtttcg aactgacctc tgtctggttc atcgggccca ggtggtacag cgcctccagg
     2461 acggagaatt ggctggtcgt caggtctccg agacagccgc acgcgaagag cctggagcct
     2521 acggactcca tcgccctcgt cagctttatg aaggcgtcca gcgccaggac ttccttctcg
     2581 tcgcctctgt agtgtgtcgg cacttcttgt tcacgtcgga tagtttgatg tcgaaccaat
     2641 cgcatatgac gtgcccacgc atctccacga agaaaggggg cgcgatttcg cgcccccttt
     2701 gctgtcacga tcgctcgttg gattgcctca ggacgaaacc aggcttccgt acagcgacag
     2761 ggtcttccgg gcgatggcgc tccagctgaa atgctcctcg gcccgctgcc tgcccgcctt
     2821 ccccatccgc tcctggagcg ctggatcgag cccaatgcgg ttgatcgcgg cggcaagttc
     2881 gcgcgagtac ctggccggac taactggctc gaagtcgtcc tcttccaggc ggggatcgac
     2941 gaggaggcca gtctcctcag gcaccacaac ctcgggaatc cctccgacgt tcgacgccac
     3001 cacggctgtc tcgcaggcca tcgcctccag gttgatcagc ccgaatggct catacaccga
     3061 tgggcagcag aacacggcag cgtgcgagta gaattggatc aggtctggga gcggcacctt
     3121 ctccaggaac cagaataccc cctcccgctt cgcgcgcacc tcgccggccc actcggtcat
     3181 ctcctgccct atctcttccg tatctgggga gttggcgcaa agcacgacct gcaaagacgg
     3241 gtcgatctcc ggtatcgccc tgacgaggtg catgatgccc ttctggcggg tgatcctccc
     3301 cacaaagagc acgtatggac ggttagggtc gatgccccgc tcgacgagtg catccgtact
     3361 gggatcgtag cggaattcct cgaggtctat gccgttgtgg atgacgtgca ccctgtgagg
     3421 cgagacgttg aaaaagcgaa ggacgtcctc cttaacctct ttggagacgg cgatgatggc
     3481 gtccgcgtcc tcgatgctcg tgtgctccat ccaggtgctc aagtagtagg cgttgcccaa
     3541 ctgctccacc ttccatggcc gccgcggctc caacgagtgg atggtcagca ccagcggggt
     3601 atcccacaac ttcttagcga tgaagccggc catgtccgtg taccaggtgt ggcagtgaac
     3661 tacctgggca tccaggttgt ccttggccat ggcgaggctg cggccgaaag cgtccatggc
     3721 gcccgcgaat cgggggtccg tattctcctt ggcccccggc caggcgggat atcccttcac
     3781 tgtcagattg ccatccgcgg agtcctggtt tccgaagcaa cgcacctcga cccgagtatg
     3841 gcgagcgagt tcgcggctca agtactcaac gtgcacgcct gcaccgccgt acacattggg
     3901 cgggtactcg ttcgtgaaca gagcaacctt ctggatctcg gccatcagaa ctcctcatcc
     3961 cccgtctctt cttcctcttc cgcgggttct ctgatgatcc ccagtacttc gtcgcgcagt
     4021 tcctccatgt cctcgcgcga tgtcgtggac tccaggttca accgcatcaa gggctcggta
     4081 ttggaggcgc gtacgttgaa atgccagttg gggtaggtga ccgagatgcc gtcgatccac
     4141 tcgatcttac cgtccgagta ctcgacggcc agttcctgca tctttgccgt gggatcttcg
     4201 acggtcgagt tgatctcccc cgaaatgaag tacttctcct ccagcgagtg gagcaggtcc
     4261 gccatcgact ggccactcgt cgagatcatc tcaatcacca gaagggacgg gatgatgccc
     4321 gagtccgcga agttgaagcc cttgaagtag taatgcccgg agacctcacc ggcgaacagc
     4381 accccctcgt cggccatccg gcgcttgatg aaggcatggc ctacccgttc catcaacggc
     4441 acgccccctg cctcccgaac gaggtccggc actgcccagg aagcgcggac gtcgtagacg
     4501 atcttgctgc ctggctgctt cttcagcaga tagctaccca gcaacgccgt aaggtagtcg
     4561 ccagccacga actctccccg attgtcgatc acgaagaagc ggtcgccgtc gccgtcgaaa
     4621 gcgaatccca catccgcctt ctcggaccgg accctctgct gcagttccgc gcggttctca
     4681 ggctggagag ggtcgagccc gtggttcggc aagttgccgt ccgggtccag atacatgccg
     4741 accaacttca ccggcagtcg ggagtagaca cgctggagga cgggcccgac catgccgttg
     4801 ccggtatcga ccaccacctt gagaggcttg atggcaggta tgtcgatcag actcaggacg
     4861 gcctcgacga attgctccga caggtccatc gagcgcatcg tcccctctcg cgtcgcgggg
     4921 gcatatgcgt cctcctccac gatacggcgg aggtcctgga tgccctggtc tccactcaac
     4981 aggtagggca tctggtgaac catcttgaag ccgttgtagg ccttcgggtt gtgggaagcg
     5041 gtgaccatga tgccgggcac cttcaattgg gagcaggcga agtagtactg gtccgtgctc
     5101 accatgccga tgtcgatcac gtcggcgccc tgcttcatta ttccccgggt caccgcttcg
     5161 aacagttggg gaccggatat ccgcatatcc cgcccgacca gcacttcctt cgcctcaagg
     5221 aaggtaacga aggcgcggcc gatgccgaac gcggtctctt cgtcgagatc ggtcgggtag
     5281 acaccccgta tgtcgtaggc cttgaatatc ccagggttga ccttcaccct atctcctcca
     5341 tcactaactg acgcttcgcc cttcaacaac tacgctactc aagctgcctt gtcaagcgct
     5401 gccctgggcg cgatcgtcgc gccgctcggg acctcacgtt cccggaagtc cgcgtgcgac
     5461 accccggcgt ctatgcggca gttccggccg atcacgacgt caggcgggat ggtagctctc
     5521 ttcccgacga cggttatgcc ggaactcagg tttgccggtt cccggtcatt cggcctgttg
     5581 tcctcgccag atcctaccct cgcccccgct ccaacgtcga cgtccttgtc gatgacgcag
     5641 cggtccacca cagcgccggg gccaatcgcc gtgttggtca tgatcacaga gtcgcgtacg
     5701 acggcgcccg gttccaccag tacgcccggc gacagcacgc tgttgatcac ggttccgtag
     5761 acgcggcagc cgttggagac caggctgttc tcgatttctc caggggcgcc aaacttggca
     5821 ggagggcgtt cctccgatcg ggtgtgtatc gtccaggtgg agtcaaacag gtccaggtca
     5881 ggcttgtcgg ccagcagcgc gaggttcgct tcccagtaag cctgaactgt cccgacgtcg
     5941 acccagtagc cgtcaaacgg aaacgcgacc acccggtcgc ggtccaccat atttgggatg
     6001 atattcttgc cgaagtcatg agccgaggca tggtcgtggg catcgtcctc gagcctctgc
     6061 agcagagtct cacgattgaa tacgtagact cccatggagg ccaaggtgct gtcggattgc
     6121 ctgggcttct ccgcgaactt cacgatgcgg ttgctgccgt ccacggtcat aatgccgaag
     6181 cgatgcacct catccggccg cacgttgatg accccgaccg tcagttcagc cctgttctcg
     6241 cggtggaacc gcagcaactc acggtagtcc tgcttgtaga tgtgatcccc tgagaggacg
     6301 agtacctcgt cgcagcctat gtctgcgatg aagttgcggt tctggtagag ggcgtccgcg
     6361 gtgccctggt accagtcctg gtctgatcta cctctatacg gctgccacat ctgtacgccg
     6421 ttcggccagc gccggttgag atcccacggt tcgccgatgc cgatgtggtg catcagagag
     6481 tggggacggt actgggtaag gacggcgata ctgtagaggt cggagttgac cgcgttggag
     6541 agggcgaagt cgatgattct gtacttgcct gcgaacggca cggcgggctt cgcccgcttc
     6601 tcgctgagga cactgagcct gctcccctct ccccccgcga gtatcagcgt caacacgctc
     6661 atcgctctac cctcctgccg tcaagttgtt gtagggcggg ggattgtccc ctgccctccg
     6721 agagtctcta cccgtactgc ttgatgaact cctcggcctt catcaccagt ctccggtcgc
     6781 ctatgaacaa cggggtcctc tggtgaagtt gcgccggttg aatttcgagg attgactgcg
     6841 tcccgtcaga agcgtagccg cctgcctgct cggcgataaa ggagagcggc gctgcctcat
     6901 agaggagccg caacttgccc tgcgacttcg tttcctcctt gctgttggcc ggatagtaga
     6961 agacgccgcc cgagaggagc gtgcggtgaa agtccgccac aagggatccg acgtaccgct
     7021 gcgacatgcc ttcgccgccc tcgaatccct gcaggtagct ggtgaactgg cgtgcgcctt
     7081 ctccccagta cttctggtat ccctggtcca cgctgtagta tcgtccctct tccggaatcc
     7141 ttatgtccgg gtgcgacgca aggaattccc cgagtcccct gtccagcgta aacccgtgta
     7201 ccccgtgccc cgcgctgtag acgagcatcg tgctcgcgct gtagacgatg tagcccgccg
     7261 ccaccatgtc cctgcctacc ctgagacact cctcagtcgt cgcgaccgca ccggggtttg
     7321 tgcgccggaa gatgccgaag atggtgccca cgctgacgtt gtagttcacg ttggaagagc
     7381 cgtccagcgg gtcgtagacc aggacgtagc gggcgctcga ggcacctgcc gggacctgaa
     7441 tcatctcggc atgctcctct gacaccatgg cggcgacccg ccctccccgt gacacgaggg
     7501 tgcgcacggc ctggtcggcg agaacgtcca gcttcatcac ctcttcgccc tggacgttga
     7561 cgtcgccggt ctgccccaga atctgggcaa gcccagcgtg ggtcatctcg ttggcgatga
     7621 tctttcccgc gagggcgagg tcgtagagga gaccggtaag agccccggtc gcctcgggat
     7681 ggatccgctg ttccgccagg atgtgcgtct cgatcgtcga gagcccatcg acatcgggga
     7741 ttcgggtcgc catgatgcct ccctatgctg cccttctcgc cactaccact tccggttctc
     7801 tctcttccac cgcttccgca acaggccgct tcgccacgta gagtgccccc accgtgacga
     7861 gagtcccaac cactatggct atcaggtaca tcccgaggtt tccgactgct cccgggatga
     7921 ggatggcgaa tatgccaccg tgggggacga gaagcttgca gcctgccagc atcgagatcg
     7981 cgcccgtgac cgccgaaccc agcatgttgg aagggatgac tcggaacggg tcgcgagcag
     8041 cgtacgggat cgctccctcg gtgatgaaag cgaggccgag cacgagggcc gcgtttccgg
     8101 cctcccgctc gtcgtggatg aagcgatcct tcatcaggta ggtagcgatt cccagcgcca
     8161 gtggtggcgt catgcctgcc gccatcacgg cggccatcgg ttcgtatacc tggctggcca
     8221 gcagtccaac ggacaacatc cacctgccca gaaacctcga gggactgaag cccgtactga
     8281 tcctcccgtt cctcggcacg ctggtagtcg gcctggccat gatttacgtc atcggcacgc
     8341 cggtgcaggc gattctgcag gccctcacca ct
//
LOCUS       DATALS010000471         9133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_443335, whole genome shotgun sequence.
ACCESSION   DATALS010000471 DATALS010000000
VERSION     DATALS010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443335"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(467..1285)
                     /locus_tag="VHS06_10500"
     CDS             complement(467..1285)
                     /locus_tag="VHS06_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2988585.1"
                     /translation="MKLLLMARFTFREALHKRVVHGVLGLTVAFLVLYGLLVWLAFHQ
                     LGPGGSASMISLQFVLLGMNAVSGIGALLAVFLSVGTISSEIEAGTLHAIMPKPLTRR
                     DVLWGKWLGFAAMLSIYVFLLALAVSMTVAFFSGEWSPQIVPAAALLVLQSLVLLSLS
                     ILGTTFLPTVANGVAVFGLYSVAMMSGFMEQLGSMIRNDAMVTVGIIVSLLVPSDSMG
                     KLSASLLQEGRSYLERVGPFMVASQPSAWMVVYSFIYMAVMLLLALRIFSKRDL"
     gene            complement(1282..2226)
                     /locus_tag="VHS06_10505"
     CDS             complement(1282..2226)
                     /locus_tag="VHS06_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988586.1"
                     /translation="MSVALNRSLAGDLAIEMIHLRKEFGRKVAVEDLSLRVPRGEIFG
                     FLGPNGAGKTTSIKVLMGLVSATSGEARLLGLPPQDLRAKARIGFLPELFRFHEWLTA
                     EQFLDFHGQLYGMPPEKRRKRIPEVLELVGLRVETKNLLRNFSKGMQQRIGLAQAILN
                     DPDVVLLDEPTSALDPLGRRDVRDLMRYLKDEGRTVFLNSHLLSEVELVCDRVAIIDR
                     GRVIATGALQDLLAASMEIEVDAEGVTPEALEELRSNSLSAEMEGGRLIVRLRERESI
                     PQIAETILRHGGRLYSLSQRRGSLEELFIRVVEQEEAR"
     gene            complement(2459..3649)
                     /locus_tag="VHS06_10510"
     CDS             complement(2459..3649)
                     /locus_tag="VHS06_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX2988587.1"
                     /translation="MGCVDSGELRAFLDGELTGAARARVQAHLAGCGGCRSELEALRA
                     NASVAQSAFDLLAPSTAEMPRLQWSQIRDRAEDRRDRPVTRVWGLNEMFAGFLRFAGG
                     SRVRMATSSIAAVVVVALVFTLSPVQTMASSFLSIFRVQKFVAVQVDPSAMKKFEPPA
                     DMGKVTGTGSGTTVRAANQAEAEKITGLHLPLPAKVPAGLAPTPRAFTVTETSSFTYT
                     PDLKKLRAYLSSIGANNVRLPDNLDGAPITMQMPPTEMATYLEPDDWANGSVRRDEDG
                     KPVPSVGQKFLYVGATTSPSLSVPDGLDVEQIRNELLKIPGLPTDMVGQLRSISDWRS
                     TMVVPVVKGSTREVTVQGQTGLMISQRDGTATSIIWPNNGAIYAVSGSYSEAEMLAVA
                     NSLK"
     gene            complement(3633..4214)
                     /locus_tag="VHS06_10515"
     CDS             complement(3633..4214)
                     /locus_tag="VHS06_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2988588.1"
                     /translation="MQVTDARLLAGLHSGEEAAFESLFSRHWRTVYSILFRLVGRREE
                     AEDLTQEVFVRLFRTPPDLRDQLTLAPWLYRVATNLGYNALRKGAREANRQEKVQRLS
                     DVEEAAGLRATDPEAASLAEEERRGVRAALSQLSEREQACLVLRHSGLSYAEVAAALG
                     VQTGSVGTILARAEAHFRKVYQGLQEAPDGMRG"
     gene            4683..6740
                     /locus_tag="VHS06_10520"
     CDS             4683..6740
                     /locus_tag="VHS06_10520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2988589.1"
                     /translation="MNSTQDTLSSHRPTALTGLGIPLSLLLIVASSVSGALVFRLALF
                     GPDTGIVTVDRRILLTLMVGALLSLWAALFALLIRRVNSYSWERALYWTAVCQTPMLI
                     GLAVVSACLSSNVAWRGFSSSETGRLLLQPMIADLLIGATAAAQLLLLALLKRRLLLL
                     HLPIIGILLVGLALRVSHLGWGLPGLLHPDEHRYLGPAIIMAARGDLNPHYFQNPSLM
                     IYLSYLVLLVMAPQSRAFLTAGSFFSLGVPNPRGDFLDMLAIRGIEAVAGTLTILAVY
                     LAARELLDRRAALMSALLLAVSFLHVRNSHYATNDILATALMAFSFYFAARIYTRGRA
                     SDYLLAGLLGGLATSAKYNVGLFAAAIVVAHLARFVHTGLSAYPWHRHLLLPAAGILS
                     VLAFLAGTPYAILDYPSFAADFSSQFGYGADVWNGQQSQPTSMLFLSALEWGLGFVPL
                     ILAVAGTLLMARRDGWKMAILLAVPLTYFAFMSSQKLFFARFALPALPFLCILASYAM
                     WRLGQTRWHLFSAVLLFAAIAQPVAMTIQHDRLLGRPDTRFLAAQWIDANIPHDATMA
                     VESYSTLDPKFGWRGHAVKDSWLFWPENEQSVAKAIGGNYRYVVVSDFGYGPWQKDGA
                     VASTLPGPYASLESRGRMVAQFAPGPGNTVLPYAIDEMYTPFWHLFDRERPGPTIRIY
                     EIR"
     gene            complement(6818..8803)
                     /locus_tag="VHS06_10525"
     CDS             complement(6818..8803)
                     /locus_tag="VHS06_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX2988590.1"
                     /translation="MARIFHTQQGTILVTAAIASAVVVAAAALFVLLPPRTVSGQVVD
                     VATGRPLLGAVVKVGGREIQVGSDGGFTISGVRIGSRVEVEAKGHQPTSSLVALGDQL
                     RLALAPRVLEGVARDASASKPVGGVKVSAGSVVVQSDAQGKFRLEGIDPGIDIEALAT
                     GYGRLTMKYDGQASGDLVLKPNVLSITAVDRYTGKPLEGVAVTDGKNTVQTDAVGQAK
                     LEYLPDGAEVTAKREGYGEFKLTFSGQDTVQAMLRPDTVIGSVKDAGGKPIAGASVSD
                     GNSTVSTDAQGAFQIEGVPENAKLTISANGFQRQQLDVNQQGSIDVVLKPFFARGLYL
                     TYYGVGDDGLRNHVLDMAAQSEINAVVIDVKGDRGWISYKSSVPMVGQIGAQQEVTMK
                     DPKGLLADLKKRGVYTIARIVAFKDNPLATARPDLAVMNSATGQPWVDNEGLRWADPT
                     KEEVWDYDIALATEAIDLGFDEVQFDYVRFPTDASAGNSLSSITFSKPNTMAVRTAAI
                     NGFLEKARQAIKAKGGSISADIFGYVVWRDDDMGIGQHLEDLAQHVDYVSPMVYPDLF
                     WDGIAVDGGAKYGNRQAGLYPYEIVNESMKVAVRRIGAAKLRPWLEYYNDYITGKTYT
                     AEDIQVQKKATYENGVNGWLFWDPSNRFDKGGFDPKQ"
BASE COUNT         1722 a         3046 c         2622 g         1743 t
ORIGIN      
        1 ctcagcagga actgcagcag gccgacgatg cggtccagaa gctgccgccg gagcaggcag
       61 cgcagcttcg taacgccatc ggggcagcca gagaggcact atcgggagac agttccaaga
      121 tggacgcggc caagaagcag atccagcaga tgatcccgcg gtaaggcagg tatccgttgg
      181 gggtcgggag gttcggctga tctgcacgca tccagccaga agggcacggc gtgatctcat
      241 cgtagggcgg gggcctgtcc cccgccgccg gcgtggcagg attctgcatg ctagttcata
      301 gccacggtgt gggcggcggg gcagagcgcc ccgccctacg ggacgccaga ggcggcaaat
      361 gaccgctcgg tagccgtggc aacgatgtgg ggggcggggc acaagacccc gccctacgag
      421 acgtcagcgg tggtatccac cggccgcatg atccccaagt gatccgctac aggtctcgct
      481 tggagaagat ccgcagggcc agtagcaaca tgaccgccat gtagatgaag gagtagacca
      541 ccatccatgc agaaggctgg gaggccacca taaaggggcc aacccgctcc aggtagctgc
      601 gcccctcctg caacagagag gccgatagct ttcccatgga gtcggacggg accagcaacg
      661 agactatgat cccgacggtc accatggcgt cgtttctgat catggagccc aactgttcca
      721 tgaagccgct catcatcgcg acggagtaca ggccgaagac ggccacaccg ttagcgacgg
      781 tgggcaggaa cgtcgtgcca aggatggaaa gggagagcaa cacgagggac tggagcacca
      841 gcagcgccgc ggcgggcacg atctggggcg accactcacc gctgaagaac gcgaccgtca
      901 tacttaccgc tagcgccaac aagaagacgt agatagagag catcgccgcg aagcccagcc
      961 actttcccca caacacatct cttcgagtca gcggcttcgg catgattgcg tggagggtgc
     1021 ccgcctctat ctcggacgag atggtgccaa ccgagaggaa tacggccagt agcgcgccga
     1081 tcccgctgac agcgttcatc ccaagaagaa cgaactgcag ggagatcatg ctggccgacc
     1141 cgccgggtcc gagctgatgg aaggccagcc agaccagcag cccgtagagg accaggaagg
     1201 ccaccgtgag gccgagcaca ccatgaacca ccctcttgtg caacgcctcc cggaaggtga
     1261 agcgggccat cagcaacagt ttcatcgcgc ttcctcctgc tccaccactc taatgaacaa
     1321 ctcctccaaa gagcctctcc tctgggagag cgagtacagt cttcccccgt gccgcaagat
     1381 ggtctccgcg atctggggga tggactcacg ctccctgagt ctgacgataa gccggcctcc
     1441 ctccatctcg gcagacaggc tattggatct caactcctcc agagcctcgg gcgtcacacc
     1501 ctcagcatcc acctctatct ccatggacgc cgccaaaaga tcctggaggg cccccgtggc
     1561 gatcacccgc cccctatcga tgatggcaac gcgatcgcag accagttcga cctcggaaag
     1621 gaggtgggag ttcagaaaga ccgtccggcc ttcatccttg agatagcgca tcaggtcgcg
     1681 aacgtccctg cggcccaacg ggtccagggc ggaggtcggc tcatccagca gcaccacgtc
     1741 ggggtcgttg aggatcgctt gagccagccc aatccgttgc tgcatccctt tcgagaagtt
     1801 gcgcagcagg ttcttcgtct cgacacgcag ccccaccaac tccagcacct ccggtatgcg
     1861 cttcctccgc ttctccgggg gcataccgta cagctggccg tggaaatcga ggaactgctc
     1921 cgccgtcagc cactcgtgga accgaaacaa ctcaggaagg aaaccgatgc gagctttggc
     1981 tcgcaagtcc tgggggggca gtcccaacag tcgcgcctcc cctgaggtgg cgcttacaag
     2041 tcccatgaga accttgatgg aagtagtctt ccccgctccg ttggggccga gaaacccgaa
     2101 gatctcgccc cgaggcaccc tgagggaaag gtcctccacc gccaccttgc gcccgaactc
     2161 cttgcgaagg tggatcatct ctattgccag atcgcccgcg agcgacctat tgagggctac
     2221 actcatcaat tgactccaag aaggagtatc atcccaccgc aggctctcac atttggagct
     2281 ttgcgtgccc catacgaggc atcaccgcct actcgaaatg gtagggcggg tcgcccctga
     2341 cccgcccgga caacggtcta cgagaaggga cttgcgggtc aggggcgacc cgccctacta
     2401 gatcgaggtg tggcggctca cgccgccacc aacaaaccgg cgtcttggcc cgcagtcgct
     2461 acttcagcga attcgccaca gccagcatct cggcctcgga gtagctgccg ctcaccgcgt
     2521 agatggcccc gttgttcggc cagatgatgc tggtcgcagt gccgtcacgc tggctgatca
     2581 tcagcccggt ctgtccctgc acggtaacct ctcgggtcga tcccttgacc accggtacta
     2641 ccatcgtgct tcgccagtca ctgatggacc gaagctggcc caccatatca gtcggcaatc
     2701 cggggatctt caacagctcg ttcctgatct gctccacgtc caggccatcg gggaccgaga
     2761 gagaggggct ggtcgtggca cccacgtaga ggaatttctg tccgaccgac ggcaccggct
     2821 tgccgtcctc gtcacgtctc accgaaccat tagcccaatc gtctggctcc aggtaggtcg
     2881 ccatctcggt cggaggcatc tgcatcgtga taggcgcccc atccagattg tccggcagtc
     2941 tcacgttgtt cgcaccgatg gatgacagat aggcacgcaa cttcttcaga tcgggcgtgt
     3001 aggtaaagct ggaagtctcc gtgactgtga aagccctggg agtcggagcc agccccgccg
     3061 gaaccttcgc ggggagcggc agatgcaagc cggtgatctt ctctgcctcc gcctggttcg
     3121 ccgctctgac cgtcgtccca ctgcccgtgc cggtgacctt gcccatgtcc gccggcggct
     3181 cgaatttctt catggcagaa ggatccacct gcaccgccac gaacttctgc actcggaata
     3241 tcgaaaggaa gctgctggcc atggtctgca ccggagatag cgtgaacacc agcgcgacga
     3301 cgaccaccgc cgctatagag gaggtggcca tcctcaccct gcttccgccc gcgaagcgaa
     3361 ggaagcccgc aaacatctca tttaaccccc atactcgcgt aacggggcga tcccttctgt
     3421 cctctgccct atctcggatc tgactccact gcaatcgcgg catctctgca gtagacggcg
     3481 cgagcaggtc gaaggccgac tgggcaacgg acgcgttggc acgtagcgct tccaactcac
     3541 tccggcagcc cccgcagccg gccaggtgcg cctgtacacg cgccctagcc gcacccgtca
     3601 gctctccgtc cagaaacgct ctcagctctc cgctatccac gcatcccatc aggcgcctcc
     3661 tgaagtccct ggtacacctt gcggaaatgt gcctcggccc tggccaggat agtacccacc
     3721 gagccggtct ggacacccaa cgccgccgcg acctccgcgt agctgagacc gctgtgccgc
     3781 aagaccagac aggcctgctc cctctcagag agctgggaca gagccgcacg gacgccccgt
     3841 cgctcctcct cagccaggct cgcagcttcc ggatcggtcg cacgcagccc cgccgcctcc
     3901 tcgacgtcgg atagccgctg caccttctcc tggcgattgg cctcccttgc ccccttccgc
     3961 agggcgttat acccgagatt ggtggcaact cgatagagcc agggagccag ggtcaactgg
     4021 tcgcgcagat cagggggggt ccggaacagc cggacgaaca cctcctgggt caggtcttcc
     4081 gcctcctccc tccggccgac cagcctgaag agtatggagt agaccgtgcg ccagtgtctg
     4141 gagaatagcg actcgaaggc cgcctcctcg ccggagtgca gccccgccag cagcctggcg
     4201 tctgtcactt gcaccctgtt ctcccgatct tctatcaatg tttgccccgt ccgagtatat
     4261 aacaccgtcc tactctactt tatgacagca gccgcatgta agaggtttcg gctcaatcac
     4321 gccaccctgc agccggctcg gcccgcttcg tcgccgccgg cattccaagg atgtggggag
     4381 ggaggggatc gcaggtgagg gtcaggcgaa tggcggtccg gccttcttgt tgggaggaat
     4441 gcctggggct tagaatagcc tgtctccgac gataccccta gtgattgacc acagtggttg
     4501 tgatacgtag ggcacgtgac ggtagcgagc acgcaacgcg agcgagcgca gcgttgcggt
     4561 cacgcctgtg cgccgccgct atcgcccgga tacggtcacc gggcggcggg gcacagagcc
     4621 ctgccctaca tgtcatttcc tgtgtggtcg tgtactagtt cagactccca ggagtaagct
     4681 tggtgaactc cacccaggat accctcagtt cgcaccgtcc cacagccttg accgggctgg
     4741 gcatcccgct cagcctgctg ctcatagtcg cgtcctccgt gagcggggct ctcgtcttcc
     4801 ggctggcgct gttcggcccg gataccggca tcgtcactgt cgaccggagg atcctcctca
     4861 ccttgatggt tggggcattg ctgtccctct gggcagcact cttcgccctt ctcattagga
     4921 gagtaaactc gtactcatgg gaacgtgccc tctactggac agccgtctgc cagactccga
     4981 tgctgatcgg gctggccgtg gtgtcggcct gcctcagcag caacgttgcg tggcgaggat
     5041 tcagctctag cgaaacggga cgacttctcc tgcagccgat gatcgcggat ctgctgatcg
     5101 gtgcgaccgc cgccgcccaa ttgctgctgc ttgcactgct gaagcggcgc ctactgctcc
     5161 tccacctgcc tatcatcggc atactgctcg tcggtctggc gctgcgcgtc tcccacctgg
     5221 gatggggtct gccggggctc ctgcatccgg acgagcaccg atacctgggc cccgccatca
     5281 tcatggcggc gaggggagat ctcaaccccc actatttcca gaaccccagc ctgatgatct
     5341 atctgagcta cctggtgctc ctggtgatgg ccccacaatc gagggcgttc cttaccgccg
     5401 gaagcttctt ctcgctgggc gtgccgaacc ccagggggga tttcctcgac atgctggcca
     5461 ttagggggat cgaggccgtc gcaggaaccc tcactatcct ggccgtctac ctggcggcga
     5521 gggagttgct cgaccgccgc gccgctttga tgagcgcact cctgctggcg gtctcgttcc
     5581 ttcacgtccg caactcccac tatgccacca acgatatcct ggcaactgcc ctcatggcct
     5641 tcagcttcta cttcgcggca agaatctaca ctcggggccg tgcctctgac tacctactgg
     5701 cagggctgct gggagggctg gccacgtcgg ccaagtacaa cgtcgggctc ttcgccgcgg
     5761 ccatcgtggt ggcccacctg gcccggttcg tccacacggg cctatcggcg tatccgtggc
     5821 accgccatct gcttctgccg gctgccggca tcctctccgt cctggctttc ctggcgggaa
     5881 cgccctacgc catcctggat tacccctcct tcgcggccga cttcagcagt cagttcggat
     5941 acggggcaga tgtctggaac ggacagcaat cgcagccgac ttcgatgctg ttcctctcgg
     6001 ccctcgaatg ggggctgggg ttcgtcccgt tgatcctggc ggtcgcggga acgctgctga
     6061 tggcacgtcg agacggatgg aagatggcca tcctgttggc cgtccccctt acctacttcg
     6121 ccttcatgtc ctcgcagaag ctgttcttcg cgcgcttcgc gttgccggca ctgcccttcc
     6181 tctgcatttt ggcaagctac gcaatgtgga ggctcggtca gaccagatgg cacctcttct
     6241 cggcagtgtt gctgttcgcc gccatcgcac agccggtagc catgaccatc cagcacgacc
     6301 ggctcctcgg tcggcccgac acccgcttcc tggccgccca gtggatcgac gccaacatcc
     6361 cccacgacgc caccatggcc gtggagagct actcgacctt ggatcccaaa ttcggctggc
     6421 gagggcatgc ggtgaaggac agctggctct tttggccgga gaacgaacag agcgtcgcga
     6481 aggcaattgg ggggaactac cgctacgtgg tggtgagcga cttcggatac ggcccgtggc
     6541 agaaagacgg tgccgtcgct tcaacgctgc ccggcccata cgcttcgctt gagtccagag
     6601 gacgcatggt agctcagttc gcccctggac ccggcaacac ggttctgccc tacgccatcg
     6661 acgagatgta cacccccttc tggcacctct tcgacaggga gcgccccggc ccgaccatac
     6721 gcatctacga aattaggtga cgaaggcggg tccagggcgc tagcgcctgg cggggtttgg
     6781 ggtgtcccca aaaccatagt gtcctattgc ttatcctcta ctgcttcggg tcgaaccctc
     6841 ccttgtcgaa acggttgctc ggatcccaga agagccagcc gttcaccccg ttttcgtagg
     6901 tggccttctt ctgaacctgg atgtcctccg ccgtgtaggt cttccccgtg atgtagtcgt
     6961 tgtagtactc caaccagggc cgcaatttgg cagccccgat ccgcctcacc gcaaccttca
     7021 tgctctcgtt cacgatttcg tacgggtaga gtccggcctg ccgattaccg tacttggctc
     7081 ccccatccac ggcaatccca tcccagaaaa gatccggata caccatgggc gaaacgtaat
     7141 ccacgtgctg cgccaggtcc tccaggtgct gtccgatccc catatcgtcg tcgcgccaga
     7201 cgacatagcc gaagatatcc gcggagatac tcccgccctt cgccttgatc gcctgccgcg
     7261 ccttctcaag gaagccgttg atggcagccg ttcggaccgc catggtattg ggctttgaga
     7321 aggtgatgga cgacagggag ttgcctgcac tggcatcagt cgggaagcgc acgtagtcga
     7381 actgcacctc atcgaatccc aggtcgatgg cctcggtggc caaggctata tcgtagtccc
     7441 agacctcttc cttggtaggg tccgcccaac gtaacccctc gttgtccacc cacggctggc
     7501 cggtggcaga attcatcacc gccaggtcgg gccgagccgt agcgagcggg ttgtccttga
     7561 acgccacgat tcgggcgatg gtgtaaacac ctcgtttctt gagatcggcc aggagcccct
     7621 tggggtcctt catggtgacc tcctgctgcg caccgatctg ccccaccatc gggacggaac
     7681 tcttgtacga tatccagcca cggtcgcctt tcacgtcgat gacgactgcg ttgatctcgc
     7741 tctgtgcggc catatccaga acgtggttcc gaagaccatc gtcgcccacg ccgtagtaag
     7801 tgagatagag ccccctggcg aagaacggct tcaacaccac atcgatgcta ccctgctgat
     7861 tcacgtccaa ctgctgccgc tggaagccgt tggcactgat agtcaacttc gcgttctccg
     7921 gcacgccctc aatctggaag gccccctgcg cgtctgtgga gacggtcgag ttcccgtcag
     7981 agactgaggc gcccgcaatc ggcttccccc cggcatcctt gaccgacccg atgaccgtat
     8041 ccggccgcag catcgcctga accgtatcct ggccactgaa ggtcagcttg aattcgccgt
     8101 aaccctccct cttcgcggtc acttctgcac cgtccggcag gtactccaac ttcgcctgcc
     8161 caacggcatc tgtttgaacc gtgttcttgc cgtcggttac ggccaccccc tccagcggtt
     8221 tgccggtgta ccggtccaca gcggtgatgg aaagcacgtt gggcttcagc accaggtcgc
     8281 cgcttgcctg tccgtcatac ttcatggtca gcctgccgta gcccgtcgcc agcgcctcga
     8341 tatcgattcc ggggtctatc ccctccagcc tgaacttccc ctgcgcgtca ctctgtacca
     8401 ccacagagcc cgcgctcacc ttcacaccgc cgaccggctt ggacgccgat gcgtccctgg
     8461 ccacaccctc aaggactctg ggagcaagcg caaggcgcag ctggtcgcca agagccacca
     8521 ggctggaggt aggttggtgc cccttcgcct caacctccac cctgcttccg atcctgacac
     8581 ccgatatggt gaagccgccg tcgctgccaa cctgaatctc ccggccccca acctttacca
     8641 ccgcccctag taacggccga ccggtagcta catccactac ctgtccgcta acggtccttg
     8701 ggggaagtaa gacgaagagc gctgctgcag cgacgactac ggccgatgct atagcagcag
     8761 taaccagaat ggtaccctgt tgagtgtgaa atatgcgtgc caaaggcttc tccatgagac
     8821 tctaaagagt acgttaacct gttgggcctg ttagaaaagg ggtggtagaa aagcaggaag
     8881 cgttcccgct cgttcgcggt tgggtccggg gatgaacgcg aggtgatacc gaggaacgcc
     8941 aaagctagat taagtcagcg atctacaggg agcaatcacc cgatagtcct agaggggacg
     9001 caacaagtgg tgttgaagcc gttcttcgcc agggggctct atctcactta ctacggcgtg
     9061 ggcgacgatg gtcttcggaa ccacgttctg gatatggccg cacagagcga gatcaacgca
     9121 gtcgtcatcg acg
//
LOCUS       DATALS010000472         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_120345, whole genome shotgun sequence.
ACCESSION   DATALS010000472 DATALS010000000
VERSION     DATALS010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120345"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="VHS06_10530"
     CDS             complement(<1..1215)
                     /locus_tag="VHS06_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988591.1"
                     /translation="MSVVSDVSILICLLLIITTAAMAGSARDTADIMPPAAFAQATGH
                     PLQAEMDAFVSRQASSASFEGSGLRNSDYLRLIQKQVKAFRPCQDSTGAIIDPVQKIE
                     WQYSTPCYALSVALLASTGYDRDPELLASGIKAMSCSVDEMHEYRCAHNHGEFFIQPV
                     MLALDLYAKIVPTERWKQKLRTLDPYKLYPDNLRRKKVCYNHNVVALAGEYLLIKDGL
                     DGDQAFFDEHMAHQRVYISRRGMYKDQDPNNPIVYDEFTRQFIDSILVEGYSGPDRDF
                     YCDGMWRGAWTSLFMQSPFGECPAGGRSAQHIWNESASAVTYEIYAAQYARKGRMAEA
                     GAFKRAAHLSLRCIERWYRPDGSGYVVKNRFPIEARYGYESYSAQSQYNLLACWLMAV
                     AHLYADDAIAERP"
     gene            complement(1378..>1725)
                     /locus_tag="VHS06_10535"
     CDS             complement(1378..>1725)
                     /locus_tag="VHS06_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX2988592.1"
                     /translation="GGRTGLQTIYEDSDPKYLKAEIDTYWIQHGGGDAAAWIEKLKGR
                     IPLVHLKDMTVREGKPIMAEVGEGNMNWTAILAACKKAGVEWYIVEQDTCERDPFESL
                     AISLKNLKSWGLK"
BASE COUNT          333 a          511 c          539 g          342 t
ORIGIN      
        1 ggggcgctcg gcgatggcat cgtcggcata tagatgcgct accgccatca gccagcaggc
       61 aagcaaattg tactgcgact gagccgagta tgattcgtat ccgtagcggg cctcgatggg
      121 gaaccggttc tttacgacat agccagagcc atcggggcga taccagcgct cgatgcacct
      181 gagcgagagg tgagccgcgc gcttgaatgc accagcctca gccatcctgc ccttgcgggc
      241 gtactgggcg gcgtagatct cgtaggtaac ggcggaggcg gattcgttcc aaatatgctg
      301 agcgctgcgg ccgcccgcgg ggcactcgcc aaacggggat tgcatgaaga gcgaagtcca
      361 cgcgccgcgc cacatgccgt cgcagtagaa gtcccgatca gggccggagt acccctccac
      421 caggatgctg tcgatgaact gccgggtgaa ctcatcgtag acgatcgggt tgttcgggtc
      481 ctggtctttg tacatgccac ggcggctgat gtagaccctc tgatgcgcca tatgctcgtc
      541 aaagaaggcc tggtcgccgt ccaagccgtc cttgatcagc agatactccc ccgccagcgc
      601 gaccacgttg tggttgtagc agaccttctt gcgccgcagg ttgtccggat agagcttata
      661 ggggtcgagc gttcgcagct tctgtttcca ccgctcagta ggaacgatct tggcgtagag
      721 atcgagcgcg agcatcacgg gctggatgaa gaactccccg tggttgtgtg cacatcgata
      781 ctcgtgcatc tcgtccacgc tgcagctcat cgccttgatg ccgcttgcca gcagctcagg
      841 atccctatcg tagcctgttg acgcgagaag cgcgacggac agagcgtagc agggagtgga
      901 gtactgccac tcgatcttct ggacaggatc gattatcgcg ccggtcgagt cctggcaggg
      961 gcggaaggct ttgacctgct tttgaatcag gcgcaggtaa tccgagttcc ggagcccgga
     1021 cccctcaaag ctggcggagg atgcctggcg ggagacaaac gcgtccatct cagcctgcag
     1081 cgggtggccg gtggcctggg cgaacgcggc gggaggcatg atatctgccg tgtccctcgc
     1141 cgacccagcc atcgcggcgg tggtaatgat cagcagcagg caaataagga tggatacatc
     1201 agagaccacg ctcacgggag tcctccgggt ggggcaggaa agcccggaaa cgtcagtccg
     1261 gggcaggaat gccccggact atcaggacct tatggatgtg gtaggatcct ccagaacccg
     1321 aacgtgcgga tgcccgaatt cggcgtctgg agacgcctcc tacaacacga aaggcttcta
     1381 cttcagcccc caggacttca ggttctttag cgagattgcg aggctctcga acgggtcgcg
     1441 ctcgcaggtg tcctgctcga cgatgtacca ctcgacgcca gccttcttgc aggcagcgag
     1501 aatggctgtc cagttcatgt tgccttcgcc gacttcggcc atgatcggct tgccctcgcg
     1561 cacggtcatg tccttgaggt gcacaagcgg aattcgaccc ttgagcttct ctatccacgc
     1621 agccgcgtcg ccaccgccgt gctgaatcca ataggtgtcg atctcggcct tgaggtactt
     1681 gggatcgctg tcttcgtaaa tcgtctgcag accggtgcgg ccgcc
//
LOCUS       DATALS010000473         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_443539, whole genome shotgun sequence.
ACCESSION   DATALS010000473 DATALS010000000
VERSION     DATALS010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443539"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VHS06_10540"
     CDS             <1..603
                     /locus_tag="VHS06_10540"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2988593.1"
                     /translation="LRDVAAEVEIVANALLLDADSTVWEIGTGTGEMALGLAARCSRV
                     WATDVSPAMLEAARTKASVRGLLNVAFGSGGFLDGFSPDHTVDGIVTQLALHHLPDFW
                     KVAALRRIRSFLAPGGRLFLKDVVFRGDLADYDGFFSDVVDQLRATAGDRMAGNTIAH
                     IREEYSTFDWILEEMLARTGFRIVDKVAHGFPTDYTCVAL"
     gene            complement(705..1496)
                     /locus_tag="VHS06_10545"
                     /pseudo
     CDS             complement(705..1496)
                     /locus_tag="VHS06_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
BASE COUNT          277 a          545 c          509 g          318 t
ORIGIN      
        1 ctcagagacg tcgcggcgga ggtcgaaatc gtggccaacg cgctgctgct cgacgcggac
       61 tccacggtgt gggagatagg aaccggtacc ggcgagatgg cgttgggact cgcggcccga
      121 tgctcgcggg tgtgggcgac cgacgttagc cccgccatgc tggaggccgc ccgcacgaag
      181 gcttcggtgc gtgggctgct gaacgtcgcc ttcggatccg gcggctttct cgacggcttt
      241 tcgccggatc acaccgtcga tggcattgtc actcagcttg ccctccacca ccttcccgat
      301 ttctggaagg tggccgcgct gcggcgcatc aggagcttcc tggcccctgg agggcggctc
      361 ttcctcaagg acgtcgtctt ccgcggcgac ctggcggact acgatggctt cttttctgac
      421 gtcgtcgatc agctaagggc gacggcgggc gacaggatgg caggaaacac catcgcccac
      481 atccgcgagg agtactccac gttcgactgg atactggagg agatgctggc tcgtacgggc
      541 ttccggatag tcgataaggt ggctcacggc ttcccgacag actacacgtg cgtggcgctc
      601 tgagtcgacg ccattttcgc agtccacttc agcgtctctt caggggtgtt ccgcgggggc
      661 gatcgagcgg agtaccagtg agctcggtac aggatcggcg aggatcaatt gcctggtacg
      721 tgaagtgccc acttcctctc tagaacgaac cgagaggtga cacccttcgg ctcctcgttg
      781 ttaacagtca cgatgcctgc cgcggctatc ctgttggcta ttgagggagt ccgccatgag
      841 aacatcccac gccgtcgcca tcctctgcgg ccttgtccta ctccttgtcc tgctgtcttg
      901 ggtcgcaccg ctctcccgca ccagtgcttc cctcccgcct gctcttgggg gccacgtaag
      961 cgctgcaggc cggcctcggc aaccaccggt gcatggcgcc gcaggatatc ccgaagcccc
     1021 tgactctcct cggagacgtt gtgcaactcg tcccgaagcc gtgaagagaa gatgggtggg
     1081 tggccgcgac gcccccgaaa ggtgggcagc gtgatcaacg agtcccgccg caggtgtgag
     1141 tcgatcaggg ccgccagggt ctgcgcgcgg cgcggttggt ccaccgcatg aaccaggatg
     1201 gcagacacgg attgaggcag tgcctcgact cccacgcgga tggagccggc ctttcccgcc
     1261 ttgtagaggg ggttctcgac caggatcaac ccctcgaaag gcttcacctc cgaccgcacc
     1321 atctcggcct cgtggcccag caccacagcg atgtctgcaa tccccgctgc tctcaactgg
     1381 gccagctgct aggctatcaa ggtagtccca aaccagggga gaagcgcctt gagctgcccc
     1441 atccggcgcg attcgcctgc ggacagcagg atcgccccaa cagccgtctc agccatggca
     1501 gactacacct ctcatcgatt tgccctcagg ctgctatgcg ccctttctcc agccggaatg
     1561 agtaggtatc ccccctgctc aggtagcgga ccctgtcctg gagccccgcc acctccacct
     1621 cggtctggta gtccgacagc ggggatttg
//
LOCUS       DATALS010000474         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_304608, whole genome shotgun sequence.
ACCESSION   DATALS010000474 DATALS010000000
VERSION     DATALS010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304608"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VHS06_10550"
     CDS             complement(<1..483)
                     /locus_tag="VHS06_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX2988594.1"
                     /translation="MLDSRWAGVRNLLLVRLDNIGDVVLLSAAIRTLKENLPDSRLTL
                     MASPAGAKTIPLLPWIDDLLVWRAIWQDVGGKMPLDPDRELQLVTDLRGRRFDAAIIF
                     TSFSQGPHGPAYACYLAGIPLRLGESKEFGGGLLTTELRNLPDDLYQGERSLRLLEGA
                     G"
     gene            complement(476..1552)
                     /locus_tag="VHS06_10555"
     CDS             complement(476..1552)
                     /locus_tag="VHS06_10555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX2988595.1"
                     /translation="MGARNVLLVRLDNLGDVLLCTPAFHAVRETLPDARLTLLASPIG
                     AQVAELNPDLDGAIVYQAPWMDAWGTLKQDGERELAMVDLLREQRFDAAIIFTSFRQS
                     ALPAAYLCYLAGIPLRLAASIDGPGSLLTTRVKQQEDPRRHEVMRALELVGVEGFHTD
                     QTDLVLQAPETERALAAAWLDELGVPSGGPLVVVHPGCSMPARTYPWELFAEVADLLV
                     ERLGATVVFTGDASEVELVDKVRGRMKRNAYSAAGESTFRSFAALVSLADLVITNNTG
                     PAHVAAALKTPVVDLFALTNPPEQWRPWNVPHRLLNKQVPCALCYSRVCPYDQECLRG
                     VSPSEVVEAARDLLREIAEGGTDA"
     gene            complement(1560..2204)
                     /locus_tag="VHS06_10560"
     CDS             complement(1560..2204)
                     /locus_tag="VHS06_10560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2988596.1"
                     /translation="MSGARAVFLDRDGTLIRNHHYGCDPDSIEFLDGVVDGLRLLKLA
                     GYKLVVVTNQSGVARGYFTEECLLAMHRRLGELLEREGVSIDGFYYCPHHAEGVVPQY
                     SHVCDCRKPEPGMVLRGCEELAIDPSHSWLIGDTLDDVEAGKRAGCQAILLDVGTEGP
                     PESPERTPDYVAKGFREAVYHILEQRYAKVGIRSPKSREKQQALARKLAEYYRG"
     gene            complement(2201..>2428)
                     /locus_tag="VHS06_10565"
     CDS             complement(2201..>2428)
                     /locus_tag="VHS06_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2988597.1"
                     /translation="EEAEGLMAEEVLTVQEVAEMLKITPATVYAWCRAGKLPAFKIGQ
                     QWRIRARALEEMMRHQGLPEGAETETGKDHR"
BASE COUNT          464 a          847 c          696 g          421 t
ORIGIN      
        1 tcccgcccct tccagtaacc gcagactcct ctctccctgg tacaggtcat cgggcaggtt
       61 gcggagctct gtcgtaagca atccaccgcc gaactccttg gactcgccaa gccgcaacgg
      121 gatacccgcc agatagcagg cataggccgg cccgtgggga ccctgggaga agctcgtgaa
      181 gatgatcgcg gcgtcgaagc gtcgccctct caggtcggtc accaactgca actccctgtc
      241 tggatccagc ggcatcttcc cccccacgtc ctgccagatc gcccgccaca ccagcagatc
      301 gtcgatccat ggaaggaggg gaatcgtctt ggcaccggcg ggactagcca tgagcgtgag
      361 tcgggagtcg ggaaggttct ccttcagggt acggatggcg gccgagagca gcacgacatc
      421 gccgatgtta tctaaccgca ccaacaggag gttgcgcact ccggcccagc gactgtcaag
      481 catcggttcc accctccgca atctcgcgca acaggtcacg cgccgcttcc accacctccg
      541 agggtgatac tccccgcaaa cactcctgat cgtaggggca gactctgctg tagcagagag
      601 cacatggaac ctgcttgttc agcagccgat ggggaacatt ccacggccgc cactgttcgg
      661 gaggattggt gagcgcaaac aggtccacga ccggggtctt cagggcggcg gcaacgtggg
      721 cagggcctgt gttgttggtg attaccaaat cggccaggga aaccagggcg gcaaagctgc
      781 ggaaagtaga ctctccggcg gccgagtacg cgttccgttt catacggccg cgcaccttat
      841 ccaccagttc gacctccgag gcgtcgccgg tgaagaccac cgtggcccct aaccgctcca
      901 ccagcaggtc ggcaacctcc gcaaacagtt cccaggggta ggtgcgcgcc ggcatgctgc
      961 agccaggatg caccacaacc aggggtccac ccgacggcac gcctagctca tccagccagg
     1021 cggccgcgag cgcccgttcg gtctccggag cctgcagcac cagatcggtc tggtcggtgt
     1081 gaaacccctc gacgccaact aactccagcg cccgcatcac ctcgtgccgc cgcggatcct
     1141 cctgctgctt gactcgagtc gtcaggagcg aacccggccc atcgatggaa gccgccaggc
     1201 gaagcgggat tcctgccagg tagcagaggt aggcggcggg caacgcgctc tgcctgaagc
     1261 tggtgaagat gattgcggcg tcgaaccgct gctcacgaag cagatcgacc atcgccaact
     1321 cccgctcacc gtcctgcttc aacgtccccc aggcatccat ccatggcgcc tggtacacga
     1381 tggctccgtc gagatcggga ttgagttccg ctacctgagc gccgatggga ctggccagta
     1441 aggtcagcct ggcatccggg agggtctctc gcacggcatg gaaggccgga gtgcagagca
     1501 atacgtcgcc caggttgtcc agcctcacca gcagtacgtt tcgtgccccc atccagcgac
     1561 tatccacggt agtattccgc cagcttgcga gccagggcct gctgcttctc gcggctctta
     1621 ggggagcgaa tgccgacctt ggcgtagcgc tgctccagga tgtggtacac cgcctcccga
     1681 aaccccttcg caacgtagtc cggcgtccgc tcagggctct ccggcggacc ttccgtgccc
     1741 acatccagca ggatggcctg acaccctgcc cgcttgcccg cctcaacatc gtccaaggta
     1801 tcgccgatga gccacgagtg gctggggtcg atcgcaagtt cctcacaacc gcgaagcacc
     1861 attccaggct ccggcttgcg acagtcgcag acgtggctgt actgcgggac taccccctca
     1921 gcgtggtgcg ggcagtaata gaagccgtcg atactaacgc cctcccgctc cagcaactct
     1981 cccagccgac ggtgcatcgc cagcaggcac tcttcggtaa agtagccccg cgcgactccc
     2041 gattggttgg tcaccacaac cagcttgtag ccggcaagct tcaacaacct caggccatcc
     2101 accaccccgt ccagaaactc gatggagtcg gggtcgcagc cgtagtgatg gttgcggatc
     2161 aaggtcccgt ccctgtccag aaacacggcc cgcgcgccgc tcatctgtga tccttccccg
     2221 tctcagtctc ggcaccctct ggaagcccct ggtgccgcat catctcctcc agtgcgcggg
     2281 cgcgaatccg ccactgctgc ccaatcttga aagccggcag cttgcccgct cggcaccacg
     2341 catagacggt ggcaggggta atcttgagca tctcagccac ctcttgtacg gtcaaaactt
     2401 cctcagccat cagcccctca gcctcctc
//
LOCUS       DATALS010000475         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_352210, whole genome shotgun sequence.
ACCESSION   DATALS010000475 DATALS010000000
VERSION     DATALS010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352210"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VHS06_10570"
     CDS             <1..853
                     /locus_tag="VHS06_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2988598.1"
                     /translation="VIPGARLLPPSSLHPFGTDALGRDMLSRIAEGAWLAARIALFSV
                     AISLVCGLVLGSLAGYYSGWLDQLLSRLMDGWLALPGALVAVVIVARLGPSLDNLILA
                     LGIMGVPSFYRIVRNTTLSARKMPYAEAAAALGATDHRIMWHHVFPNILSPVIVLTSM
                     RLGTVLLTGSSLSFIGLGAQPPAPEWGSLLATGRNYLGTAWWLAIFPGMAMTFTVVGL
                     NMLGDGLRDMLDPRGGRTGRMDGAKDTGHQRETEIEQRGMEHGGLGEEVIAGRSRKIP
                     VAVEPDV"
     gene            complement(933..1637)
                     /locus_tag="VHS06_10575"
     CDS             complement(933..1637)
                     /locus_tag="VHS06_10575"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988599.1"
                     /translation="MARDGFEVVVSTPATVAENVALDEEMTKAAASSGCSTLRLWWAG
                     APTVVIGRSEIPEVVANLDACHGLGIEVIRRATGGGTVLQMPGVLNYSITTPAPSLFD
                     IRRTFAVGARYLIRVLAEFGLEGMQRGISDVAVGDLKVSGNAMAKRWGGLLLHGTLLY
                     DVDLDLVETCLRHPPREPDYRAGRGHRQFITTLKALGVTASDVDLERVAAEVARQMAE
                     AGELRLPGGSGTGEPG"
     gene            complement(1639..1845)
                     /locus_tag="VHS06_10580"
     CDS             complement(1639..1845)
                     /locus_tag="VHS06_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988600.1"
                     /translation="MAHWIVRTKGEYPHLLPHLVEEVVSKKWVGEDEMLWEVEMTEME
                     AEAVAQRAFAQSVEPAHASGRLQR"
     gene            2140..3474
                     /locus_tag="VHS06_10585"
     CDS             2140..3474
                     /locus_tag="VHS06_10585"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEX2988601.1"
                     /translation="MNEGEHRRLLVLNEVNRGRLTAWRAGELLGLSERQVRRILAAYR
                     EEGAAALVHGNRGRRPSNALDEGLRQRVAQLARTKYAGFNQLHMTEMLAEEEKLEIGR
                     TSIRRILLAEGIASLRKRRAAKHRTRRERYPVEGMLLQIDGSSHDWLSGRGPAMCLVA
                     GIDDATSTVPFGVFRPTEDTIGYFLLIEGVIAKKGRPTAVYRGRHSIFETSSREPTLS
                     EQLRGRLDPTQFGRMLQELEIESIPARSPQAMGRIERLWGTFQDRLVAEMRLKGISDI
                     DEANRFLPGFLARYNRRFAVPASEEGSAYRPLPEGLDPERVFCFKHTRTVGADNVVAY
                     QGRRLQILPSTERASYARLEVEVHEHLDGRIALFYRDRRLAVRPAPAEAAELRSRTSR
                     VDRAQLPSTDRAGKPAKPPGNPAWGRTFKLRGSRPNRPMDQEPSLAATARHS"
BASE COUNT          772 a         1227 c         1194 g          695 t
ORIGIN      
        1 ggtcatcccg ggggcacgtc tcctgccgcc gagctcgctg catccctttg gcaccgacgc
       61 gctcggcaga gacatgctga gccgcattgc cgagggagcg tggctcgccg cccgcatcgc
      121 gctttttagc gttgccatct ctctggtgtg cgggctggtc ctgggtagcc tggccggtta
      181 ctactccggc tggttggacc agttgctctc gaggctgatg gatgggtggc ttgcactccc
      241 cggcgcgctc gtcgcggtcg tcatcgtggc gagactgggg ccatcgttgg acaacctgat
      301 cctagccctt gggatcatgg gcgtcccatc gttctaccgc atcgtgcgca acaccacgct
      361 gtccgcccgt aagatgccgt acgccgaagc agcagcggca cttggcgcca cggaccaccg
      421 cataatgtgg caccacgtct tccccaacat cctctcgccg gtaatcgtcc tgacctcgat
      481 gcggctcgga accgtcctcc tcaccggcag ctcgctcagc ttcattgggc taggagctca
      541 gcctcctgcc ccggaatggg gatctctgct cgccaccgga agaaactacc tcggcactgc
      601 gtggtggctg gcgatcttcc ccggcatggc gatgaccttc acggtagtcg ggctcaacat
      661 gctcggcgat gggctgcggg acatgctgga tcctcgtggc gggcgaacag ggcggatgga
      721 tggcgctaag gatacggggc accagaggga aaccgagata gagcaacgtg gtatggagca
      781 cggcggcctt ggggaggagg tcatcgcggg ccgatcacgc aagatcccgg tcgctgtcga
      841 acccgacgtc taagaagcga caaaacagga taatcaggat agcaaggatg gccaggacgg
      901 attcataaag agtctatcct ggccatctgc gattatcctg gctctcctgt tccggaaccc
      961 ccaggcagcc gcagttcacc ggcctctgcc atctgccgag ccacttctgc cgcaaccctc
     1021 tcaaggtcga cgtcgctggc agtgacgccc agggctttca gcgtcgtgat gaactgtctg
     1081 tggccgcggc ctgcccggta gtcgggctct ctgggagggt gtcggaggca tgtctcgacg
     1141 aggtcgaggt cgacgtcata cagcaaggtg ccgtggagga gaaggccacc ccatcgcttc
     1201 gccatcgcat tgccagagac tttcaggtca cccacggcaa cgtcggaaat gccgcgctgc
     1261 ataccttcca gaccgaactc cgccagcact cggatgaggt atctggcgcc aacggcgaag
     1321 gtccgacgga tatcgaagag agaaggagca ggggtggtga tagagtagtt gaggacaccc
     1381 ggcatctgga gtacggtccc gccgccggtt gcgcgtcgta tcacttcaat gccaagaccg
     1441 tggcaggcgt ccaggttcgc cacaacctcg ggaatctcgg acctaccaat cacgacggtg
     1501 ggcgcacccg cccaccagag acgtaaggta gaacaccccg acgaggcagc cgccttcgtc
     1561 atttcctcgt cgagggcaac gttctccgcc accgttgccg gagtggagac aactacctcg
     1621 aacccgtccc tcgccaactt acctctggag gcgtcctgat gcgtgggctg gctcaacgga
     1681 ctgggcgaag gcccgctggg caactgcctc ggcctccatc tccgtcatct ccacttccca
     1741 cagcatttcg tcctcgccta cccacttttt ggagaccact tcctccacga ggtgtggcag
     1801 caagtgaggg tactcgccct ttgtccgcac aatccagtga gccattatcg ttacctcctc
     1861 gtcgcctgag catgcaatcg tcacagtctc gggcaccgtg ggtccggctc gactctgaga
     1921 acggactatc attgcgtcga acagattcct cagatctcgg cagtctcaag gcctgggaaa
     1981 cccgccattt tagagaaacc tgtctatgtg acgctagcgt cattctagtg gatctccagt
     2041 tgccccgcaa tggctcatct tgaggctgtc gttggccagg gaatctgaca cggtgtaatt
     2101 gttcagcgaa aatgtcaccc catgagggag acgatcacgt tgaacgaggg cgaacacagg
     2161 cgactgttgg tgctgaacga ggtgaaccga gggagactga ctgcctggag ggcgggggaa
     2221 ctgctcggcc tgtcggagag gcaggtgagg aggatactgg cggcctatcg ggaggagggg
     2281 gccgcagccc tggtccacgg caaccgcggg aggaggccgt ccaatgcgct ggatgagggc
     2341 ttgcggcagc gggtggcaca gctcgcccgc accaagtacg cgggcttcaa ccagctgcac
     2401 atgaccgaga tgctggccga ggaggagaag ctggagatag gcaggacctc catcaggcgc
     2461 atcctcctgg ccgaggggat cgcaagtctg aggaagaggc gagcggccaa gcacaggacg
     2521 agaagggagc gttatcccgt ggaggggatg ctcttacaga tcgacggctc ttcccacgac
     2581 tggctctcgg gaaggggtcc tgcgatgtgc ctagttgctg ggatcgacga tgctaccagc
     2641 accgtcccct tcggcgtctt caggccgacc gaggacacga ttggctactt cctcttgatc
     2701 gagggagtga tcgccaagaa gggcagaccc acggccgtct accgaggccg gcattccatc
     2761 ttcgagacct cctcaaggga gcctacgctc tccgagcaac tccgaggcag gctcgatccc
     2821 actcagttcg gccgcatgct ccaggagttg gagatcgagt ccatcccagc gaggagccct
     2881 caggctatgg ggaggatcga gaggctctgg gggaccttcc aggatagact ggtcgcggag
     2941 atgcgcctta aggggataag cgacatcgat gaggccaacc gcttcctgcc aggcttcctt
     3001 gcccgctaca accgccgctt cgcggtgcct gcctccgagg agggctcggc ctatcgtccg
     3061 ctacccgagg gcctggaccc cgagagagtc ttctgcttca agcacacccg tacggtcgga
     3121 gccgacaacg tcgtcgctta ccagggtcgc aggctgcaga tccttcctag caccgagagg
     3181 gccagctatg cccgcctgga ggtagaggtg cacgagcacc tcgacgggag gatcgcccta
     3241 ttctatcgag accgtcgcct tgccgtgaga ccagcccctg ccgaggcggc ggaactgcgc
     3301 tctcggacat cacgggtaga ccgagcgcag ctaccatcga ccgacagggc gggcaagccc
     3361 gcgaagccac ctggcaatcc cgcctggggc aggaccttca agttgcgcgg gagcaggccc
     3421 aacaggccga tggaccagga gccatccctg gctgcgacag cccgccattc gtgaacgggc
     3481 tctacttgca ggcacaagag cttcccacta acctgtcgcc tgtccggcca aacggaggaa
     3541 cgcaacgctg gagagcgacg tgccgaggga taaccggatg gggcgacgga tatccgtcga
     3601 gggggaggac ccgaccaggc cctcccctgc cagtcctagg atatcccgag cccccaatgc
     3661 ctcataaact caccaatcga tggcagcagg catcactcca aaaaggccgc gctacaccac
     3721 gggatgacaa agtcgctgga caattaaccc tgacagaatc gctggccaac aacacggctc
     3781 atcttgaggc aaagttaacg tcagtatccg ggatacggct cctcaatcct cagtcctcga
     3841 ctctggcaaa tcggacacga tttccgtcct ccaccatcac cttcccga
//
LOCUS       DATALS010000476         5034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_352246, whole genome shotgun sequence.
ACCESSION   DATALS010000476 DATALS010000000
VERSION     DATALS010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352246"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1013)
                     /locus_tag="VHS06_10590"
     CDS             complement(75..1013)
                     /locus_tag="VHS06_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2988602.1"
                     /translation="MPLKCVVTGAAGFIGSHLSEALVQRGHDVVGIDCFTDYYPRPAK
                     ESNLATLRQSPRFAFVEANLLEYDLNSLLDGVEYVFHQAAQAGVRASWGQNFSVYTEN
                     NVLATQRLLEAAKSHPIKRLVYASSSSIYGDAKELPVTEETIPQPISPYGVTKLAAEH
                     LCRLYWVNFRVPTVSLRYFTVFGPRQRPDMGFHKFFRALLRDDEITLYGDGDQTRDFT
                     FVSDAVAANLACMEAPVTGEVFNIGGGSRISVNRVLELMQAISGRKARIQRLEEQKGD
                     VRHTFADTSRAREALGFRPSVSTEEGLRAEWEWISH"
     gene            complement(1004..2149)
                     /locus_tag="VHS06_10595"
     CDS             complement(1004..2149)
                     /locus_tag="VHS06_10595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2988603.1"
                     /translation="MSTSDSGSFKILHLVTSLDLGGAQNHLLSLVKGLRARGHTADVA
                     FFKNPTLLKEFQATGAEVFDLSTRRTFSPLLIPRLTGILRDGRYQILHTHLLKADSYG
                     AVAGMLARTPVVLSSKHNDEIALQNPRVATVHGLLSRLDRKVIVLSDYVGRYVSSVGK
                     VDRTRIDRVYYGIQPTTAATAEDGARVRAELGIPAEAPLIATVGRLMEQKGLVYLLQA
                     MKILGDRLPEARLLVVGDSQDGRDGYKMELLKQWESMGLQGKVVFTGVRHDIPAVVQA
                     IDLFAMASLWEGFGLVFLEAMAAARPIVATEVSAVPEVVENGVTGLLVPPRDPQALAD
                     AMYRLLTERDRATAMGEAGLLKLKQQFSEDKMVQAIEQIYSQLWRCH"
     gene            complement(2146..3387)
                     /locus_tag="VHS06_10600"
     CDS             complement(2146..3387)
                     /locus_tag="VHS06_10600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2988604.1"
                     /translation="MKVLVLSDYYPPNTKGGADIAAERLSAEIAHRGHSVCALTTVQE
                     KSQAVSDTIRGVQVHRFYSSYPIELHNYMAVYNPMVVGQVRKKIEEFKPDVVHAHNVH
                     THLSFQTLLEAKKRSIPVILSAHDHQLFCSGKFDCLPPEKATAVTVSQCAHCQQLRFF
                     PLRNRLIRHRVEESGARILAVSAALRDDLVANGFAPETVGVMHNGIDPNSMAVSDEHV
                     RMFALRHELVGKKVILFGGRVSHAKGIDQAVSALGRLPRDLNFVFLVLGSSEDYISYI
                     LQLGHRLSVEDRTVFLPWLSGEDLKAAYAISDVCLTPSMYREPFNLINIEAMALKKPV
                     ITTCFGGPPEVVVDGVTGYVLDPRNADLMAARLMELLSDERKAKAMGEAGYRRVEEKF
                     TVAYQADQTLAAYAAALENQA"
     gene            3478..4872
                     /locus_tag="VHS06_10605"
     CDS             3478..4872
                     /locus_tag="VHS06_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX2988605.1"
                     /translation="MNPSTTSPNGNLPGGTLISKLTAAPRRLLGRRFAREVLTLQVGS
                     FATMGIQFVTSVVIANLLGPHPLGIYYQAKALLDLVNMLANLAVGQALITPLAAAHTQ
                     RNRDEARCILAYFLKMGLTVSFVTSAVGLLAGNYLGAIFLDDPQIGDMARVLFVTPPL
                     LVIFNMATLALQSSRQVGRLTLLENGYLMSTSLLNVAVVALGGGVNELLYSVSFAALL
                     TAVASLWMYSNALSKMPSFPTLMEIAKSAPGIPFRRYFVFSALVSVDKNFANLISLAP
                     TILLGRWGTSADVAYYKVAANLTVTLLSVPMSPVSRNLYAKLAEVRVKAPQRLGRMLV
                     QVTLGSLAISGGMALGLMLAAPFIIQIYRPDYLPALGAMYALGVRCATLGFGVGLGPL
                     YQVLGKMKLAILSKVPPALVMLVGGWFLIPSLGAVGGAWTVTLTYLVGDIISACLMWW
                     ILTRNRAAVQAAKA"
BASE COUNT          908 a         1562 c         1534 g         1030 t
ORIGIN      
        1 gcgggaaggg ggggctgggg acaccccaga ccccgacaga gagggacctg cggccccctc
       61 tgcactcccc cagcttagtg gctgatccat tcccactcgg ctctgaggcc ctcctcggtg
      121 gaaaccgagg gacggaaccc cagtgcttcc ctcgcacggc tggtatcggc gaaggtgtga
      181 cgcacgtcgc ccttctgttc ttccagccgc tggatccttg ccttccgacc ggagatggcc
      241 tgcatcagct ccaacactcg atttaccgag atgcgggagc cgccgccgat attgaacacc
      301 tcgcctgtca ccggcgcctc catgcaggcc aggttggctg ccacggcgtc gctgacgaag
      361 gtgaagtctc tggtttggtc tccgtccccg tagagggtga tctcatcgtc tctgagcaat
      421 gctcgaaaga acttgtggaa acccatgtcc ggacgctgtc gaggaccgaa gacggtgaag
      481 tagcgaagcg acacggtggg cacgcggaag ttgacccagt atagtctaca caggtgctct
      541 gccgccagct tcgtgacccc gtagggggat atgggctggg gaattgtctc ctccgtcacc
      601 ggcaactcct tcgcgtcgcc gtatatggag gaggacgaag cgtagaccag tcgcttgatc
      661 gggtggctct tggcggcttc caggagccgc tgggtggcca gcacgttgtt ctcggtgtag
      721 acgctgaagt tctgtcccca actggcgcgc acaccggcct gggccgcctg atggaagacg
      781 tattccacgc cgtcgagcag actgttcagg tcatactcca gcaggttggc ttcgacgaag
      841 gcgaagcgtg gagattgtct cagggtggcc agattcgatt ccttggccgg cctggggtag
      901 tagtcggtga agcagtcgat acccacgacg tcgtgcccgc gctgcacgag cgcctcagat
      961 aggtgggatc caatgaaccc ggctgcgccg gttaccacgc acttcaatgg catctccata
     1021 gctgagagta gatctgttcg atggcttgga ccatcttatc ctctgagaac tgctgcttca
     1081 acttgagaag ccctgcctcg cccatggctg tagctcgatc ccgttcggtc agcagtctgt
     1141 acatcgcatc tgctagagcc tgggggtctc tggggggaac cagcagcccc gttaccccat
     1201 tctcgacgac ttcaggcacg gcgctgacct ccgtggcgac tatggggcgc gcagcagcca
     1261 tcgcctccag gaacaccaga ccgaatccct cccagagaga ggccatggca aacaagtcta
     1321 tcgcctgcac cacggcgggg atgtcatggc gaacgccggt gaaaaccacc ttaccctgga
     1381 gtcccatgga ctcccactgc ttgagcaact ccatcttgta gccgtcccgg ccgtcctgcg
     1441 agtcgccgac cactagaagc cgggcttcgg gcagccggtc cccgaggatc ttcatcgcct
     1501 gcagcagata caccagtccc ttctgctcca ttagccgccc aacggtggcg atcaacgggg
     1561 cctcagcggg gatacccaac tcagccctca ccctcgcgcc gtcttcagcg gttgcggccg
     1621 tcgtgggctg gatgccgtag tagactcgat cgatgcgtgt gcggtcaacc tttcccacgg
     1681 aggagacgta tctgcctacg tagtccgaca gcacgatcac ctttcgatcc agccgcgaca
     1741 gcagtccgtg aaccgtggca acgcgagggt tctgcagggc gatctcgtcg ttgtgcttgg
     1801 aggagagaac caccggtgtc cgagcaagca ttcctgccac cgcaccgtag ctatccgcct
     1861 tgagcaggtg ggtgtgaagg atctggtagc ggccgtcgcg caatatccca gtcaaccgtg
     1921 ggatcagcag cggggaaaag gtccggcggg tggagagatc gaagacttca gcccctgtgg
     1981 cctggaactc cttcagcagt gtcgggttct tgaagaacgc cacgtcggcc gtgtggccac
     2041 gcgcccgcag cccctttacc agcgacagca ggtgattctg cgccccaccc aggtcgagag
     2101 aggtgaccag gtggagtatc ttgaaggacc cactgtcgga cgtgctcacg cttggttttc
     2161 cagggccgcc gcataggcgg ccagcgtctg gtctgcctgg tacgccacgg tgaacttctc
     2221 ctcgaccctc ctgtatccgg cctctcccat cgctttggcc ttcctctcgt cggagagcag
     2281 ttccatgagc ctggctgcca tcagatccgc gtttctggga tccaggacat atccggtgac
     2341 gccgtcgacc actacctcgg gaggacctcc gaagcaggtg gtgattaccg gcttcttcag
     2401 tgccatcgcc tcaatgttga tcagattgaa gggctcacga tacatcgagg gggtgaggca
     2461 gacgtcgctg atggcatagg cggccttcag gtcctccccg gagagccacg ggaggaagac
     2521 ggttcgatcc tccacgctca gtctgtgacc tagctgcagg atgtaggaga tgtagtcttc
     2581 gctgctgccc agcaccagga agacgaagtt caagtcccga ggcagtctcc caagagcgga
     2641 caccgcctga tcgatccctt tggcgtgact caccctgccg ccgaagagaa tgaccttctt
     2701 ccccaccagt tcgtggcgta gcgcgaacat tcggacgtgc tcgtcgctca cggccatact
     2761 gttggggtcg atcccgttgt gcatgacgcc gacagtctcc ggcgcaaaac cgttggccac
     2821 gaggtcgtcg cgtagcgcgg cgctgaccgc aaggatgcgg gccccggatt cttcgaccct
     2881 gtgccggatg agcctgttgc ggagggggaa gaatctgagt tgctggcaat gggcgcattg
     2941 agagaccgta accgctgtgg ctttctccgg aggtaggcaa tcgaacttcc cactgcagaa
     3001 gagttggtgg tcgtgtgctg agaggatcac agggatcgat cgcttctttg cctccagtaa
     3061 ggtctggaaa gagaggtgcg tgtgcacgtt gtgggcgtga acgacatccg gcttgaactc
     3121 ctcgatcttc ttgcggacct ggcctacaac cattgggttg tacacggcca tgtagttgtg
     3181 gagctcgatg gggtaggacg aatagaaccg gtgaacctgg acacccctga ttgtgtcgct
     3241 gacggcctgt gacttctcct gcaccgtggt taaggcgcaa acagaatgcc cccggtgggc
     3301 gatctcggct gaaagccttt cggcggcaat atcggcaccc cccttggtgt tggggggata
     3361 ataatccgag aggacaagta ccttcatggt tccgtggtgc tcctgatccg cgtcattgcg
     3421 gtcgctccag atttggtatt atacctcagc agccgcgctg ggctaaagga tgccaccttg
     3481 aatccttcga ccacctcccc caacggcaac ctccccgggg gcaccctgat ttctaaactg
     3541 accgccgccc cgagacgatt gctgggtcgc cgctttgcta gagaggttct caccctccag
     3601 gtaggtagct ttgccaccat ggggatccag ttcgtcacgt cggtggtcat cgccaacctg
     3661 ctgggtccac atcccctcgg tatctactac caggcgaagg cgttgctgga cctggtaaac
     3721 atgctggcga acctggccgt tggacaggca ctcatcactc cgttggccgc agctcacacc
     3781 cagcgaaacc gcgatgaggc gcggtgtatc ctggcctact tcctcaagat ggggctcacc
     3841 gtcagtttcg tcacatcggc cgtcggtctg ctggcgggca actatctggg cgctatcttc
     3901 ctggacgatc cccagattgg ggacatggcc agggtgctct tcgtgacccc gcctctgctt
     3961 gtcatattca acatggcgac cctggcgctg cagagttctc gccaggttgg gcgcctgacc
     4021 ctattagaga acggctacct catgtccacc agcctgctca acgttgcggt ggtggccctt
     4081 gggggtgggg tgaacgagct tctctactcg gtgagcttcg ccgccctgct cacggccgtg
     4141 gcgtcgctct ggatgtacag caacgctctc tccaagatgc ccagctttcc caccctgatg
     4201 gagatcgcca agtcggcacc tgggatcccg ttccggcgct attttgtctt cagcgccctc
     4261 gtgagcgtgg acaagaactt cgccaacctg atctccctgg ctccgacgat tttgcttggg
     4321 cggtggggca ccagtgcgga cgtggcgtac tacaaggtgg ccgcgaatct caccgttaca
     4381 cttctttccg tgccgatgtc tccggtctcc agaaacctgt acgcgaaact ggcggaggtt
     4441 cgggtgaagg cgccgcagcg gctgggccgg atgctggtgc aggttacgct ggggagtctg
     4501 gccatctcgg gtggtatggc gttggggttg atgttagcgg cgcccttcat catccagatc
     4561 tacaggcctg actacctgcc ggcattgggc gccatgtacg cacttggggt tcgttgtgcg
     4621 acgctgggct tcggcgttgg gctggggcct ctctaccagg tgctggggaa gatgaagctg
     4681 gccatcctct ccaaggtccc gcccgccctg gtgatgctcg tagggggctg gttcctgatc
     4741 ccctccctgg gggccgtggg tggggcgtgg acggtgacgc tgacctacct ggtgggggac
     4801 atcatcagcg cctgcctgat gtggtggata ctcactcgaa acagagcggc agtgcaggcg
     4861 gcgaaggcgt agacgcggag aggaaggtcc tgacctactg cccggccacg cccaccgagt
     4921 ggcactctac acggcccagc cgctcccaaa gttggccagc tggtacaatc cttcccagca
     4981 cccagcaccc agcacccagc acccagcacc cagcacccag cacccagcac ccag
//
LOCUS       DATALS010000477         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_305191, whole genome shotgun sequence.
ACCESSION   DATALS010000477 DATALS010000000
VERSION     DATALS010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305191"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VHS06_10610"
     CDS             <1..202
                     /locus_tag="VHS06_10610"
                     /inference="COORDINATES: protein motif:HMM:NF040055.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988606.1"
                     /translation="RGPGEYFGVRQSGFPDFRMADLGDAKLVGAAREAAAELLRRDPD
                     LSRPEHRLLAGRVEELRRAESS"
     gene            219..833
                     /locus_tag="VHS06_10615"
     CDS             219..833
                     /locus_tag="VHS06_10615"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEX2988607.1"
                     /translation="MRVIAGVARGRPLRSIESRAVRPTSDKIKGAIFSILESAAFKRG
                     MVEPGYLARVEEGELAFPWKRVLDLYAGSGALGIETLSRGAEHVDFVEADAGAREAIS
                     QNLKKTGLADRARIHGLKVENAISTFNVPYDLILLDPPYDDPAFVSVFQRLCESAVVG
                     IHTFVVLEHSRQRAVEPQCGPLVLLKTRVHGRTGISVYAAGQQE"
     gene            837..1358
                     /gene="coaD"
                     /locus_tag="VHS06_10620"
     CDS             837..1358
                     /gene="coaD"
                     /locus_tag="VHS06_10620"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX2988608.1"
                     /translation="MRIAIYPGTFDPFHDGHMDIALRASRLFDRLIVAVYEYPGKDVL
                     FNAQERLEIARRAMAPYPSISVELFSGLAVEYAKTQQACALVRGLRAISDFEYEFEIA
                     HMNRRLNPDVEAVFLMTSLPYAFLRSSLVKEIAKLGGSLEGLVSDYAKEALTGKYEAR
                     ARAGGLPTSNLVP"
     gene            1408..1884
                     /locus_tag="VHS06_10625"
     CDS             1408..1884
                     /locus_tag="VHS06_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX2988609.1"
                     /translation="MLTSGSRIPLTGKTVVDEHECLDIIDQLRVAVPEEVKQAKRMQS
                     DRERVIQEAEERASRIVAHAQEQASRLAEQHEVARAAEAAARRILAEADAEAAERKDG
                     ADRYAAETLAELEHRLDELLGVVRNGLRSLDHDDAPAAPSQRVVVGYDDDLDGNPL"
     gene            2047..>2255
                     /locus_tag="VHS06_10630"
     CDS             2047..>2255
                     /locus_tag="VHS06_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX2988610.1"
                     /translation="MIGFNIAQLLKAPTGTIRRVEVDELDPELAADLHLVSPIRGSLK
                     LMRTTAGVLVEGKLTYEIELNCSRCL"
BASE COUNT          445 a          591 c          788 g          431 t
ORIGIN      
        1 gcgtgggcct ggcgagtatt tcggtgtgcg gcagagcggc tttccggact tcaggatggc
       61 ggacctgggc gacgcgaagc tggtgggggc ggctcgagag gccgcggcgg agttgctgcg
      121 gcgcgacccc gacctgtcga ggccggagca taggcttctg gcgggcaggg ttgaggagtt
      181 gcggcgagcg gagagctcgt gaggaggaac agtcgagaat gcgtgtgatt gccggggtgg
      241 cgcgagggcg gccgctgagg agcatcgaga gccgcgcagt caggccaacc agcgacaaga
      301 ttaagggggc catcttctcc atactggagt ccgcggcctt caagcgtggg atggtggagc
      361 cgggctacct tgccagggtg gaggagggag agctcgcctt cccctggaag cgggtactgg
      421 acctctatgc aggtagtggg gcgctgggca tcgagacatt gagccgcggc gcggagcatg
      481 tggacttcgt cgaggcagac gcaggggcga gggaagccat ctcgcagaac ttgaagaaga
      541 ccgggctcgc agatcgtgcc aggatacacg ggctgaaggt cgagaacgca atctcgacat
      601 ttaatgtacc ctatgattta atactgttgg atcccccata tgatgatcct gcctttgtgt
      661 ctgtattcca aaggctgtgc gaatcggcag tggttgggat ccacacgttt gtggtccttg
      721 aacactcccg gcagcgggcg gttgagccgc aatgtgggcc gctcgtgctc ctaaagaccc
      781 gtgttcacgg aaggaccggg atcagcgttt acgctgcggg ccaacaggag tgaaacgtgc
      841 gtatcgccat ctatcccgga accttcgacc ccttccacga cggccacatg gacatagcgc
      901 ttcgggcgtc gcggcttttt gaccggctga tcgtcgcggt gtacgagtat cccggcaagg
      961 acgttttgtt caacgcgcag gagcggttgg agatagcccg gcgggctatg gctccgtacc
     1021 ccagcatcag cgtagagttg ttctctggac tggcggtcga gtacgccaag acgcagcagg
     1081 catgtgcatt ggtgcgcggg ttgcgagcaa tctcggactt cgagtacgag tttgagatag
     1141 cgcatatgaa tcgacgcctc aatcccgatg tggaagccgt cttcctgatg accagcctgc
     1201 cctatgcctt cttgcggtcc agcctcgtga aggagattgc caagctaggc gggtctttgg
     1261 aaggattggt gtctgactat gcgaaagagg cgctgactgg gaaatacgaa gcgcgtgcga
     1321 gggctggcgg gctgcccacc agcaacctgg tgccataggg ggacgggcta gatagatatc
     1381 ctgtacctgc tggaccgact ggagactgtg ctaaccagcg gctcccgcat cccactcacc
     1441 gggaagacgg tggtggatga gcacgaatgc cttgacataa tcgatcagct gcgagtggcc
     1501 gtgcctgaag aagtgaagca ggccaagagg atgcaatcgg acagggagcg ggtcatccag
     1561 gaggccgagg agcgagcgtc gcgcatcgtc gctcatgcac aggaacaggc gtcgagactg
     1621 gccgagcagc acgaggttgc cagggctgcc gaggccgcgg cacgacggat cctcgccgag
     1681 gcggatgcgg aagcagccga gcggaaggat ggagcagatc gctacgcggc cgagacgctg
     1741 gcggagttgg agcaccggct ggacgagttg ttgggcgtgg tgcggaacgg actgcgttct
     1801 ctggatcatg atgatgcgcc agcggcgccc agccagagag tggtagttgg gtatgacgac
     1861 gacctcgatg ggaatccttt gtagtcttgc ctttccttga cgcctactgc aggcggccgt
     1921 ataatttgca cgggcgcgcc taaggcggcc ttcttattat gcgcgacgtc tctgcattgg
     1981 gactctttgg cgaacattta ccatagggga cagacagggg ctgaacggga acggaagtga
     2041 tgtgctgtga ttggattcaa catcgctcag ttgctcaaag ctcccaccgg caccatccgt
     2101 cgtgtggagg tggacgagct tgacccggaa ttggcagccg acctgcatct ggtctcaccg
     2161 atacgcggta gcctgaagct catgcgcacc acggcgggcg tgctggtaga ggggaagctg
     2221 acctacgaga tcgagctcaa ctgctcccgt tgtct
//
LOCUS       DATALS010000478         8241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_352493, whole genome shotgun sequence.
ACCESSION   DATALS010000478 DATALS010000000
VERSION     DATALS010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352493"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VHS06_10635"
     CDS             <1..817
                     /locus_tag="VHS06_10635"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2988611.1"
                     /translation="TLATPAENREEKVIAVYPADWAEWAKSAGKAEMPQPPEEQPAAT
                     PQAVSKQDGNSTLSVGGPTSGASIRGAIDIRGTASGRAFASYKVEFGEGSNPSNWTPI
                     GGVHSAPVNNGVLERWDTKSLNGLYKLRITLTEKAVSAPSSTTPPVRQGTPSPAVPPT
                     PTPIPAGAPKVIEIPVVVDNTAPTVTVTYPTEGAVVKKDQTAKVTLTAQVADNNRVSK
                     TDFYVDDQLVGSSNAIPTTVEWTATAGPHMLRVVARDPAGNETKSADVRFQVQ"
     gene            826..1572
                     /gene="surE"
                     /locus_tag="VHS06_10640"
     CDS             826..1572
                     /gene="surE"
                     /locus_tag="VHS06_10640"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX2988612.1"
                     /translation="MTNDDGIDSEGLLALRESLSRIGETVVIAPDRNWSAAGHNKTMH
                     KPLRVTKVRMPGDIEGYSCDGTPSDCVAMSMLGLAGLRPDLVVSGINKGANLGGDVTY
                     SGTVAAAMEAVISRVPGIAVSLSSVHDQEFQVAADFAALLAQRVMEAHIDPTVLLNVN
                     VPSVPQEAIKGVLVTRLGHRVYRDQLIERKDPFGRSYYWIGGDEPTGEAEEGTDIGAI
                     ANGYISITPIHMDLTNYALIDQLKGWNLTP"
     gene            1647..1880
                     /locus_tag="VHS06_10645"
     CDS             1647..1880
                     /locus_tag="VHS06_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988613.1"
                     /translation="MMEMTGEHFKMSHDRLQELMAQEEVNAAGLSVARVREICLARSW
                     PDPTVHQGWLDEAKIGEIAHWVCFVDKQESRRD"
     regulatory      1954..2069
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            2133..3239
                     /gene="metK"
                     /locus_tag="VHS06_10650"
     CDS             2133..3239
                     /gene="metK"
                     /locus_tag="VHS06_10650"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX2988614.1"
                     /translation="MADPRSYTFTSESVSEGHPDKVCDYIADSVLDAHLEQDPHSHVA
                     CEVMCKSNMVTLAGEISSRATGIDFTAIAREAVRQVGYTDLNEPFCADTLFVNQFITS
                     QSPDIAQGVDNETDQGAGDQGIMFGYATDETPELMPLPIMLAHRLAMGLAQDRRSKAV
                     RWLRPDSKTQVSVRYEDERPVAVTDVLVSTQHDAEVAQSEIADYVKGNLVPRILDGWY
                     NPDISLQVNPTGSFLLGGPSADCGVTGRKIIVDTYGGAGRHGGGAFSGKDPSKVDRSG
                     AYFCRYVARRAVKAGLAHKVEIQVSYAIGVAKPMSVKVDTFGTGDEKAVKQFVEGFDF
                     RPAAIIERLNLLRPIYRKSTNYGHFGKAGLPWEE"
     gene            3593..3685
                     /locus_tag="VHS06_10655"
     CDS             3593..3685
                     /locus_tag="VHS06_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEX2988615.1"
                     /translation="MSSVVKKRRKKITKHKLKKRLKRERFKKNR"
     gene            3725..4126
                     /locus_tag="VHS06_10660"
     CDS             3725..4126
                     /locus_tag="VHS06_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988616.1"
                     /translation="MDKEDCIEALLSTLRKPQWKQSNSVTDWDLGNVLWRAGILFTPD
                     QMSALLSDVRRRGLITSRERRCDNRIAALWGVKLTASGEEWLNQRAQGGTVTARRALS
                     YPPSVPQAPLASPTESVDSAEADSAQEVESI"
     gene            4208..5422
                     /locus_tag="VHS06_10665"
     CDS             4208..5422
                     /locus_tag="VHS06_10665"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2988617.1"
                     /translation="MAKALTGLRVLDLTGALSGPYCTLILGDLGAEIIKIEPPDGDIF
                     RNIGPYYQGEWSGYFSAVNRNKRGIVLNLKTDAGRKAFYDLVKLSDIVLDNFRPGVLD
                     RLEITYPVLSEINPAIICCSITGWGSDTSIKHRAAFDLCVQAAGGLMSVTGQEDGTVA
                     KVGTPIGDLAAGQFAVIGILTALAERSKSGRGQHVDISMFDCQISLLAYWVPWYFLSG
                     EVPGPLGTGHLGISPHGAYRTKDGKIAMVIGSEKFWKQLCEVLGVPEIATEERFESAK
                     KRRANREELKSIIEDIMSTRTTAEWMVDMERIGIPAAPVNTLDKAFGESAAEERNMVV
                     SVEQPRGGPIHMAGNPVKMSRTPGEEFTPAPALGEHTTEILTGLLGYSPQKVEELLRE
                     GGAVQFQPSVRG"
     gene            5922..6848
                     /locus_tag="VHS06_10670"
     CDS             5922..6848
                     /locus_tag="VHS06_10670"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX2988618.1"
                     /translation="MWRPRFREAYLSHLLTAAGVSLIAVVLASGPLGHAGKASQPAQQ
                     MVASAHMASAAQTPTRPVEYVSSRGSRSSGPAQSAQATSTTPTATPIPTPTLFPTPAP
                     SPAPAPAQAPSRDRDLRVGIQAGHWKCSELPAELASLRTSTGAAGPGWKEVDVNLDVA
                     TRVQAILTEKGIQVDLIPSTVPVGYKADAFVALHGDANSNTAVSGYKLARATRSTIPA
                     KDDALLAAISHEYTASTGLKNHPGSITVNMTSYYAFANRGIAHSVAPTTPSVILEMGF
                     LTTPGDRKLLLEQPQNAAEGIAKGIMDYLGVN"
     gene            complement(6985..8093)
                     /locus_tag="VHS06_10675"
     CDS             complement(join(6985..7670,7670..8093))
                     /locus_tag="VHS06_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611722.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX2988619.1"
                     /translation="MTRKLYEVRLSPEERARLIELTKKGKVPARQLRRAHILLLAEEG
                     TKDGAIADSLHVSLSTVRRTRQRFAREKLDKALTERFRPGAKRKLDGKVEAFLVALAC
                     SDAPEGREHWTMQLLADRLVQLELVDEISDETVRRVLKKGDLKPWLKKEWCIPRVGAE
                     FVWRMEDVLDLYAEPLDACRPVVCFDERPCQLIGDVVAPIPMKPARTRKQDYEYKRRG
                     SCNLFVMVEPLAGWRNVEVTDRRTSIDFAQQMRTLVDVHYPRAEVIRVVLDNLSSHSP
                     AALYQAFPPEEARRLTRKLEFHYTPKHGSWLNMAEIELSALARQCLMRRIADKDTLLT
                     EVDPWQAERNRQKVTIHWRFSTADARTKLNRLYPS"
BASE COUNT         1699 a         2635 c         2413 g         1494 t
ORIGIN      
        1 cacgctggcc actccagccg aaaacaggga ggagaaggtg atcgcggtct atcctgcaga
       61 ttgggcggaa tgggcgaagt cagccggcaa ggcggagatg ccgcagcccc ccgaggagca
      121 gcccgccgcc acgccacagg ccgtatccaa gcaggacggc aacagtaccc tatccgttgg
      181 tggccccact tcgggcgcct cgattcgagg tgcgatagac attcgcggca cggcctctgg
      241 cagggcattt gcttcctaca aggtggaatt cggcgaaggc tcaaatccct ccaactggac
      301 gccgatcggt ggcgtccact cggcgcccgt gaacaacggc gtgctggaga gatgggatac
      361 caagtcactg aacggactgt acaagctgcg gataaccctg acagagaaag cggtctcggc
      421 cccttcatcc acgacgccac cggtgaggca ggggacacct tcgcccgctg tgcctcccac
      481 gccgacgccg atccccgccg gggcaccgaa ggtgatcgag atcccggtcg tggtcgacaa
      541 tactgcaccc accgttaccg tcacctatcc tacggaaggg gcggtagtga agaaagacca
      601 gaccgcgaag gtcacgctga ccgcccaggt ggccgacaac aaccgtgtgt ccaagactga
      661 cttctacgtc gacgaccagc ttgtcggcag cagcaacgcg atccctacta cggtggaatg
      721 gacggcaacc gccggccctc atatgcttcg cgtggtcgct cgcgatccgg ccggcaacga
      781 aaccaagagt gctgatgtga gattccaggt ccaatgagaa tactggtgac taacgacgac
      841 ggcatagact ccgaggggct gctcgctctc agagaatcgc tctcccgcat aggagagacg
      901 gtggtcatcg cgccggaccg gaactggagc gcggccggtc acaacaagac catgcacaag
      961 ccgctcaggg tcacgaaggt ccgcatgccg ggtgatatcg agggctactc ctgtgacggc
     1021 acaccctccg actgcgtggc aatgagcatg cttggactgg ccggcctgcg tccggacctc
     1081 gtggtctccg gcatcaacaa gggcgcgaac ctgggagggg acgtcaccta ctcgggcacc
     1141 gtcgcggcgg ccatggaggc ggtgatcagc cgagtcccag gcatcgccgt ctccctctcc
     1201 tccgtgcacg atcaggagtt ccaggtagca gccgacttcg ccgcgctgct cgcccagcga
     1261 gtgatggaag cgcatatcga tcccacggtc ctcctgaacg tcaacgtccc aagcgtcccc
     1321 caggaagcaa tcaagggtgt gctggtcacc aggctgggcc acagggtcta ccgggaccaa
     1381 ctgatcgaac ggaaagatcc attcggtcga agctactact ggattggtgg agacgagccc
     1441 acgggggagg ctgaggaggg cacggacata ggagccatcg ccaacggcta catctcgatc
     1501 acaccgatcc acatggacct caccaactac gcactgatcg accaactcaa aggctggaat
     1561 ctcaccccct gatcccggct ggcatgaacc gcggagacac ccgctgctgt gtggtgacgc
     1621 aatctgagga tgggagagct gagtcgatga tggagatgac aggagagcac ttcaagatga
     1681 gccacgaccg cctccaggaa ttgatggctc aggaggaggt gaatgcagcc gggctatccg
     1741 tggcacgggt gcgcgaaatc tgcctcgcca gaagctggcc cgatcccacc gtgcaccagg
     1801 gatggctgga cgaagcgaag atcggagaga tcgcacactg ggtctgcttc gtcgataagc
     1861 aggagagccg gcgagattga cggcgaagga agagcctgcg gtttgttgac caccctccgg
     1921 cccatctgat atacttcatc tactaatcga atacagcgct atcaagagtg gcgtcttctc
     1981 aaccgagacg tccggcaacc tggcggcgac cgccgaggtg cccgcccctt tcagggggat
     2041 aggcccgtaa gggaaccaag ggtcgcgaga cccccatacc cgggggttct tctttagcag
     2101 ccacgcttga tagctctgga aggagcatga gtatggctga ccccaggagc tacaccttca
     2161 cctccgagtc ggtttccgag ggccatcccg acaaggtctg cgattacatc gcggattccg
     2221 tcttggacgc acatctcgag caggatcccc acagtcacgt ggcctgcgag gtcatgtgca
     2281 agagcaacat ggtcaccctg gccggagaga tcagctctcg agccacgggc atcgacttca
     2341 ctgccattgc gcgtgaagcc gtccgccagg tcggctacac cgatctcaac gagcccttct
     2401 gcgccgacac cctgttcgtg aaccagttca tcacctccca gtccccagat atcgcgcagg
     2461 gcgtcgacaa cgagaccgac cagggcgccg gcgaccaggg cattatgttc ggctacgcca
     2521 ccgacgagac cccggaattg atgccgttac cgattatgct tgctcaccga ttggccatgg
     2581 ggctcgccca ggacaggcgg agcaaagccg tcaggtggct gcgaccggac tccaagaccc
     2641 aggtctcagt gcgatacgag gacgagcgcc ctgttgccgt gacggacgtc ctggtgtcca
     2701 cgcagcacga cgcagaggtc gctcagagtg agatcgccga ctacgtgaag ggcaacctcg
     2761 tccctcgcat cctggacggc tggtacaacc ccgatatcag cctgcaggtg aaccccaccg
     2821 gcagcttcct gcttggtggc ccgtcggcag actgtggcgt caccggccgc aaaatcatcg
     2881 tcgacaccta cggaggtgca ggcaggcatg gtggaggcgc attcagcggg aaggatccgt
     2941 ccaaggtaga ccggagcggt gcctacttct gccgctacgt cgccaggcgt gcggttaagg
     3001 cgggactggc acacaaggtg gagatccagg tttcctacgc catcggcgtc gcaaagccaa
     3061 tgtcggtgaa ggtggatacc ttcggcacag gtgacgagaa ggccgtgaag cagttcgtcg
     3121 aggggttcga cttccggccg gccgcgatca tcgagcgact caacctgctg cgcccgatct
     3181 accgcaagtc caccaactac ggccacttcg gcaaggcggg tctcccctgg gaggaatagt
     3241 tctcgacgca gtgccattcc tgttggtgca cggcaatgcc ttacagtagg gcgggtcggg
     3301 ggcgacccgc ccgcaattcg tatgttttgc actggtccgg gcggcgcaac gctgcgctcg
     3361 ctcgcgtcac ggtggcaacc caccctgcca gtaaacggac ctcccggaag acggcttgcg
     3421 cgatatacct cgccggcact tgcgcgccgc gcgccaaatt ggtaaagttg ccctgcacct
     3481 ggcctgccct ggcaggtcgg agtttttgtt gcctgttggc cggtggcatc gcgactgttg
     3541 cgtcctactc tttacccgtc ccagggtcag ctatccactt aagggggtgt gaatgagctc
     3601 tgtcgttaag aagcgcagaa agaagatcac caagcacaag ctgaagaagc gactgaagag
     3661 agagcgcttc aagaagaacc gctaacaagg ttgcccggct atcttgagat gagcaggcat
     3721 gtcaatggac aaggaagact gcattgaggc gctcctttcc accctgcgaa aacctcaatg
     3781 gaagcaaagc aactcggtta ccgactggga tctagggaac gtcctctgga gggcaggcat
     3841 cctgttcacc cctgatcaga tgtcggccct cctctccgac gttaggcgga gagggctgat
     3901 caccagtcgg gagagacgat gcgacaaccg aatcgccgcg ctttgggggg ttaagctaac
     3961 tgcctcggga gaggagtggc tcaaccagcg tgcacagggc ggaacggtga cggctcgccg
     4021 cgctttgtcg taccctccgt cggtccccca agctcccctg gcgtctccga ccgaatctgt
     4081 ggactctgcg gaagccgaca gcgcacagga agttgagtct atttgagtcg actccgtgtc
     4141 ctctggcacc gacggcagat tggtcgggca ccccagtcta gctcatcata attgggggcc
     4201 agcgtaaatg gcaaaggcgc ttaccgggct gagggttctg gacctgaccg gagccctctc
     4261 tggaccatac tgcaccctga tcctcggcga cctcggagcc gagatcatca agattgagcc
     4321 ccctgacggg gatatcttcc gcaacatcgg cccctactac cagggggagt ggagcggcta
     4381 cttctctgcc gtcaaccgca acaagcgcgg cattgtcctc aacttgaaaa cggatgcggg
     4441 caggaaggct ttctacgacc tggtgaagct atccgatata gtattggaca atttccggcc
     4501 gggcgtgctc gaccggctgg agatcaccta cccagtcctc agcgagatca accctgccat
     4561 catctgctgt tccatcaccg gatggggatc cgataccagc atcaagcatc gggcggcctt
     4621 cgacctctgc gtccaagcag cgggcgggtt gatgagcgta actggccagg aagacggcac
     4681 agttgccaag gtcggaaccc ccatcggaga cctggcggca ggccaatttg ccgtcatcgg
     4741 gatcctcacg gcactggcag agcgaagcaa gagcggccga ggccagcacg tagacatctc
     4801 gatgttcgat tgccagatct ctcttcttgc ctactgggtc ccctggtact tcctaagcgg
     4861 cgaggtcccg ggtccgctgg gcaccggcca cctcggtatc tctccccacg gtgcctatcg
     4921 cactaaggac ggcaagatag ccatggtaat cggctctgag aagttctgga agcagctgtg
     4981 cgaggtactg ggcgtcccgg agattgccac cgaggagcga ttcgaaagcg ccaagaagcg
     5041 cagggcgaac cgcgaagagt tgaagtccat catcgaagac attatgtcca cccgcacgac
     5101 cgccgagtgg atggtggaca tggagcggat aggcatcccc gctgctccgg tcaataccct
     5161 ggacaaggca ttcggtgagt ccgcagctga agagcgaaac atggttgtat ccgtggaaca
     5221 gcctcgcggc ggcccgatac acatggccgg caaccctgtg aagatgtccc gcaccccggg
     5281 cgaggagttc accccggcgc ctgcattagg cgagcatacc accgaaatcc tcacgggtct
     5341 cctcggctac tcaccacaga aggtcgagga gttgctcaga gagggcggcg cggtccagtt
     5401 ccagccttct gtccgagggt agagtcttcg ggacacgcgg ctcacgccaa tacctggaca
     5461 ataccatcgt cccgaggggt agcctgaccg gctgcccctc tcctttcctc cccagacttc
     5521 tacatccctc tcctcaacga catcggctca tccacgcctc cttcacgcac tgccggttgt
     5581 gcaaccgtgc gcacctccca taggaaagcc gatgcgcaat tcatccccct acaaatgcag
     5641 cttcacccgc ggcaccaagg ctctaagtgg cagtaatacc tcttactctc ctgtatcctt
     5701 ctaagaattg gtaccaattg gctatgctct cgcatctcgc ctatatgccc gattcaggta
     5761 taatgcatca ggcatcgtcc tcttcagcca catgattcga acccctcttc gggttcgttt
     5821 ccgacaccca cacgccaacc cagcagccga ggcctgttgc cacccaacgg ggttccgcaa
     5881 ccggccgggc tctcatctac caaccatcgg aggtacacgc catgtggcgg ccaagattcc
     5941 gcgaggcata tctctcacat ctgctaaccg ccgctggcgt atccttgata gccgtcgtcc
     6001 tggcttccgg gcccctgggc cacgcgggca aggcttccca gccagcccaa cagatggtag
     6061 cttcagcgca catggcaagc gcggcacaaa cgcccacgcg acctgttgag tacgtctcct
     6121 cccgagggag ccgatcctcc gggcccgctc agtcggctca ggcgaccagc acgactccaa
     6181 cagccacacc gataccgacg cccaccctgt tcccgactcc ggctccctcg ccggcgcccg
     6241 cacccgctca ggccccatct cgagatcgag atctaagagt aggcatacag gctggccact
     6301 ggaaatgcag cgagcttccg gcagaactgg cgagcctgag gacttcgacg ggtgccgccg
     6361 gccccggctg gaaggaggtc gacgtcaacc tcgacgttgc gacacgggtg caggcaattc
     6421 tcacagagaa gggtattcag gtggatctga taccctccac ggtccccgtc ggctacaagg
     6481 ccgacgcctt cgtggccctg cacggagatg ccaactccaa caccgctgtc tccggctaca
     6541 aactcgctcg tgccaccaga agcacgatcc cggcgaagga cgacgcgctc ctggccgcaa
     6601 tttctcacga gtacaccgcg tccaccggcc tcaagaatca tccgggcagc atcaccgtca
     6661 acatgacctc ctactacgcc ttcgcgaacc gaggcattgc ccactccgtc gcacccacga
     6721 ccccatcggt aatcctggaa atgggcttcc tgacaacgcc gggcgaccgc aagctgctgc
     6781 tggagcagcc acagaacgca gcagagggga tagccaaggg gataatggac tacctggggg
     6841 tcaactagta ctctgtcaca cggcatctga tggatggtag gggcgcacct gcgtgtgcgc
     6901 ccaggtccga gggcggacac ataggtccgc ccctaccact cgtcgatctg gctgtgacag
     6961 agcactagtg ctccgccaca gtgattatga agggtagaga cgattgagct tggttctggc
     7021 atcggcagtg gagaaacgcc aatggatggt taccttctga cgattgcgtt cggcctgcca
     7081 tgggtcaacc tccgttagga gcgtgtcttt gtctgctatg cggcgcatca ggcactgccg
     7141 ggccagcgcc gaaagctcga tctcggccat gttcagccaa ctaccatgct tgggtgtgta
     7201 atggaactcc agctttcgag tgagcctgcg agcctcctca ggagggaacg cctggtagag
     7261 ggcggcaggg ctgtgactgg acaggttgtc caggactacc ctgatcacct cggctctcgg
     7321 atagtgcacg tcgaccagcg tcctcatctg ctgggcgaag tcgatgctgg ttctcctgtc
     7381 ggtgacctcc acgttccgcc agccggccaa aggctcgacc atgacgaaca gattgcagct
     7441 gccccttcgt ttgtactcgt agtcctgctt tctagttcta gccggcttca ttggaatggg
     7501 agccaccaca tctccaatca gttggcaggg ccgctcgtcg aagcacacta ccggccggca
     7561 ggcatccaag ggctcggcgt acaggtccag gacatcctcc atgcgccaga cgaactcggc
     7621 gcccacgcgg gggatgcacc actccttctt caaccacggt ttgaggtccc ctttttcagg
     7681 acccgtcgaa ccgtctcgtc ggagatctca tccaccaact ccagttggac caacctgtcc
     7741 gccaggagtt gcatggtcca gtgttcccgt ccctcgggag cgtcgctgca agccagggct
     7801 accaggaacg cctcgacctt gccatccagt ttccgcttgg ctcccggcct gaagcgttcc
     7861 gtcagggcct tgtccagctt ctccctggca aaccgctgcc gagtgcgtcg aaccgtcgac
     7921 aggctcacat gcagcgagtc ggctatggcc ccatccttgg tgccttcctc ggccaacaac
     7981 aggatatggg ctcgccgaag ctgccgggct ggcaccttgc ccttcttggt cagttcaatg
     8041 agtcgagccc gttcttccgg cgacagtcga acttcgtata gcttccgtgt catggttctc
     8101 cgagatcctg ctggattgtc tgccaatcca aagtctaccg cctcgaccgt agcctcggcc
     8161 atggccgcgg caacgtgagg tcggacgaag gggccctcac cgatgctgcc caggataaag
     8221 atgccgtcga cgccggcatt c
//
LOCUS       DATALS010000479         7810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_444483, whole genome shotgun sequence.
ACCESSION   DATALS010000479 DATALS010000000
VERSION     DATALS010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444483"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VHS06_10680"
     CDS             <1..454
                     /locus_tag="VHS06_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988620.1"
                     /translation="VTKIPVRTEMLAKGQLQAATLPEPLASLAIQQGARLVIDDSKTG
                     TGQSVITFRQEVVSKNPDAVRRFLAAYEQGVKRIEAAPESYRDLLVDKAKVPDSLRNS
                     LRMPSYPRAQLSTREELADVVSWMVERKLLDQPIPYEKLVAEGLLPQH"
     gene            465..1310
                     /locus_tag="VHS06_10685"
     CDS             465..1310
                     /locus_tag="VHS06_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2988621.1"
                     /translation="MIEVSDLSFGYARPDEIFQNFGWHVARGESWAVLGPSGCGKTTL
                     VYLLAGLRHPSAGTVMVDGAGVTSPRTSTGLILQDHGLLPWAKARDNVALGLKIRRVP
                     RPEMDEKVDYWMERLGIAAVADRYPGQLSGGQRQRVAIARTLALSPSLLLMDEPFSSL
                     DSTTREDLQELVVSLSGGSGVTTVLVTHDIQEAVFLGQRIMIVGRAPIHGYGVVENPY
                     ARRSEYRRSPDFFQRCAEVRTLAAELMNGVLPPQGEALTCQRTCQRGEGPTSLPVDPS
                     LGGMS"
     gene            1307..2047
                     /locus_tag="VHS06_10690"
     CDS             1307..2047
                     /locus_tag="VHS06_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2988622.1"
                     /translation="MKTRQIFWATVLLVLLWQVAAVLINASVLPGPGAAVMALVYNLP
                     RGLAGHFLVSGYRLALSMFIAALLATPLGLAIGQSPRLNGFISPLIYMTYPIPKIVFL
                     PIIMLFLGTGDGAKVFMITLILFFQILVVVRDACLGVRPELVQSVRSLGAGRLELLRY
                     VYLPACLPSVLTALRVSTGTAVAVLFLAETFATQSGLAYYIMVEAWGRMAYPEMYAGV
                     IAMAALGLTTYILLDRLERRACEWMVVR"
     gene            2044..2748
                     /locus_tag="VHS06_10695"
     CDS             2044..2748
                     /locus_tag="VHS06_10695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2988623.1"
                     /translation="MTENRSHGDKGPRVREMFGRIVPRYDLMNRLMTGGMDRGWRRTA
                     ARLAAPRGMAVLDIATGTGDLAVEMVREGARSVVGLDFCGPMLEAAARKTGPFLPPIR
                     LVAGDAQHLPFEDGTFDRVTNGFLLRNVSDLPLTLREMVRILKPGGRAVCLEITHPPF
                     RPFEMAFQLYFYRLVPILGALVSGDAQAYRYLPNSLTTFPDAPSLAQMMREAGFSHVT
                     YRYLSLGAMAAHVGVK"
     gene            3026..3565
                     /locus_tag="VHS06_10700"
     CDS             3026..3565
                     /locus_tag="VHS06_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988624.1"
                     /translation="MSKREIVLGNLVSTVAVALLAVGCASSYGTPVAPTQSATSTPAT
                     LAGAPATTTPTAQPKVTPIATVGATQVPAPASASTRAPVSLTPVSAGAEPTIQVTGQV
                     RDVAASARVITFVEAVHGFEDVALTDSTQIIGPDGSSRTLQDIQPGMVVQAAGWANGS
                     GAVIAKSVKIISGPTPLPQ"
     gene            3741..6326
                     /locus_tag="VHS06_10705"
     CDS             3741..6326
                     /locus_tag="VHS06_10705"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEX2988625.1"
                     /translation="MTLEPVVWQHTPYTVLLMVAAGVAMLRAWQIWQSPMSGARLLAM
                     ILLFAGLWSFGYGLEMASANLAYKQLWVKARYIGITAVPTFWALFTLKYTGRNRWLSP
                     RPLGLLLLVPIATLMITFTDSWHSLMFARYDMIYVSGLAVLELVHGPWFVLWTAYAYG
                     LLLLGTLLLLELMVRSRQLYHWQSSLLISAAAVPWAVNVITVSDSARDAGLDFSCLVV
                     IATAMIVSWGLVRLRLDDLVPVARRNFFANTADAVLLLDGQGRILDVNPRAEQLIGTN
                     ARAVIGKSVGQVWPQWPQRMERLQEQSSSSEELTVGEGDQQRIYDVLESPVVDWRGRV
                     ACWTVVLRDITRRRNAEDALRVSEAKLLSILDSSPDAITVCDLDGTTIDCNQSALKMI
                     GLESKTQLLGANILDSVPLRERGRAGESMGQLLTEGVLRDLEFAIVTVDGREFPGELS
                     ARIIEDDSGRATAFVVVTRDISLRKQMSDQLLRAQRLESAGRIAGQVAHDFNNLLSPL
                     FAYPELIKKQLPEGHPALLCCDQMLEAAERMAYINEELLTLGRRGNFVLEPTDLNSLV
                     EIAVAEMGVRPNGLWVRTKPEPELRPVMASSAQLIRVISNLLSNAHEAMPNGGEITVA
                     TKNVQLPAPLSPNARVKLEDYVRLSVTDTGCGIPPEIVDKIFDAFFSTKRSVKRRGCG
                     LGLSVVQSIVEDHHGYLDLQSEVGVGTTFHVYLPASDEAPKTRPTEVVKGGIETILVV
                     DDDQLQRDVSAAMLNELGYRVAVASSGEEALEYLKGSTVDLLLLDMMMPPGMDGADTY
                     RRAREIRPGLKAVIVSGFVATERVREAQSLGAGQFLRKPLTLEKLAEAVRVELDCRDQ
                     PLASN"
     gene            6348..7424
                     /locus_tag="VHS06_10710"
     CDS             6348..7424
                     /locus_tag="VHS06_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX2988626.1"
                     /translation="MIEKIETAYYRLPLEPMGDAGHGAIDVEEFITLKLYADGLVGHG
                     YAYTIGRGGRAIQSLIDHDLAPLLVGRDAADVEGLWDYMWHRLLYVGRGGVASFAIAA
                     VDIALWDLRGLRECKPLYALAGGTAREIPAYGSGVDLPKPKDELLSQVEGFLKRGFPG
                     VKVKIGRPDPREDEDRVEAVRKLIGDGVDLMIDANMTWPAEVALERGKRFEPFNPFWF
                     EEPTIPEDVPGHARLVEGLVTPIALGESLHSPYEYQRYVETRAVEVLQIDPVTNGGIT
                     SSLKALAMADAAGLKTSSHYTDELCAHLLCASRNPIYLEKHAFALDPYLLQPQQVVNG
                     RVRPTEVPGTGMRFDEKAMAPFRA"
     gene            7443..>7810
                     /locus_tag="VHS06_10715"
     CDS             7443..>7810
                     /locus_tag="VHS06_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988627.1"
                     /translation="MGDASIAHTVLLKALILHFFAQTVRRRFGQDMADGSLTRRLPVG
                     AELQPEGAVHFRVWAPGRDTVELVLESGPGEGSRCIGMAPEGNGYFQLTVPGAVAGTL
                     YRYLLDGEGPYPDPASRFQPL"
BASE COUNT         1373 a         2302 c         2591 g         1544 t
ORIGIN      
        1 ggtgacgaag atccccgtgc ggaccgagat gctggccaag gggcagttgc aggcggcgac
       61 ccttcctgag ccgctggcct ccctggcgat ccagcagggg gccaggctgg tgatcgacga
      121 cagcaagacc ggcaccggcc aatcggtgat caccttccgc caggaggtag tctcgaagaa
      181 tcccgacgcc gttcgccgct tcctggccgc ctatgagcag ggggtaaagc ggatcgaggc
      241 ggccccagag agctatcggg acttgctggt agacaaggca aaggtgcccg actcgctgcg
      301 gaactctctc agaatgccca gttaccccag ggcgcagctc tctacgaggg aggagttggc
      361 cgacgtggtg agctggatgg tcgagcggaa gctgctggac cagccgatac cctacgagaa
      421 gctggtggcc gaggggctgc tgccccaaca ctgaaaggcg tacagtgata gaggtttccg
      481 atctgtcctt cggctacgcg aggccggacg agatatttca aaactttggc tggcatgttg
      541 cccggggcga gtcctgggcg gtgctgggcc cgtcgggctg cggcaagacc acgctggtct
      601 accttcttgc cggccttcgc catccatcgg cgggcacggt gatggtggat ggtgccgggg
      661 taacgtctcc ccgtacctcc acgggactaa tccttcagga tcacggtctg ctgccctggg
      721 cgaaggctcg ggacaacgtg gcattggggc taaagatccg gcgtgtgccc cgtccggaga
      781 tggacgagaa ggtggactac tggatggagc ggctggggat agccgctgtg gccgaccgct
      841 accccggcca gctatccggc gggcagcggc aacgtgtggc catcgcccgc accctcgccc
      901 tgtcgccgtc gctgttgctc atggatgagc ccttctcctc gctggactcc accacgaggg
      961 aggatctgca ggagttggtc gtttcccttt ccggggggag tggagtgacc accgtgcttg
     1021 taacccacga catccaggag gcggtgttcc tggggcagcg cataatgatc gtggggcgag
     1081 cgccgataca cggttacggg gtggtggaga acccctacgc caggcgctcg gagtaccgcc
     1141 gcagccccga tttcttccaa cggtgcgctg aggtaaggac gctggccgcg gagttgatga
     1201 acggggtctt accccctcag ggagaggcgt tgacatgcca gaggacatgt caacggggtg
     1261 agggccccac atccttgccc gtggatccct cgctgggggg tatgtcgtga agactcggca
     1321 gattttctgg gcgacggtgc tgttggtgtt gctgtggcag gtggctgccg tcttgatcaa
     1381 tgcctccgtg ctgccgggac ccggtgcggc cgtgatggcg cttgtgtaca accttccaag
     1441 agggctggcc ggccacttcc tggtgagcgg ctacagattg gccctgagta tgttcatcgc
     1501 ggcgctgctg gcgaccccac tcggactggc tatcgggcag agcccacggc tgaacgggtt
     1561 catctcaccg ctgatttaca tgacctaccc gataccgaag atcgttttcc tgccgatcat
     1621 catgctgttc ctggggacag gtgatggggc caaggtgttc atgataaccc tgatcctctt
     1681 cttccagata ctggtggtgg tgagggacgc ctgtctcggg gtcagaccgg agttggtgca
     1741 gtcggtgcgc tccctcggtg caggcaggct cgagttgctg cgctacgtct accttccggc
     1801 ctgcctcccc tccgtgttga cggcgctgcg ggtgagcacc gggacggcgg tggcggtgct
     1861 cttcctggcg gaaaccttcg ccacgcagtc ggggttggcc tattacatca tggtggaggc
     1921 gtgggggcgg atggcctacc ctgagatgta cgccggcgtg atcgcgatgg cggcgctggg
     1981 gctgacaacc tacatcctgc tggacagact ggagaggcga gcttgcgagt ggatggtggt
     2041 tcgatgacgg agaacaggtc gcatggggat aaggggcctc gggttcggga gatgttcggt
     2101 cgcatcgtgc cccgctacga cctgatgaat cggctgatga cggggggtat ggaccgaggc
     2161 tggcgcagga cggcagcccg attggcagcg cctcggggaa tggccgtgct ggacatcgcc
     2221 accggcacgg gtgacctggc cgtcgagatg gttcgcgagg gggcgcgctc cgtcgtgggg
     2281 ctggacttct gcggacccat gctggaggcg gctgccagga agaccggccc cttcttgcct
     2341 cccatacgat tggtggccgg cgacgcacag cacctcccct tcgaggacgg gaccttcgac
     2401 agggtgacca acggcttcct gctgcgcaac gtctcggacc ttccgctgac cctcagggag
     2461 atggttcgca tactgaagcc cggcgggcga gccgtttgcc tggagatcac tcacccgccg
     2521 ttcaggccct tcgaaatggc ctttcaactc tacttctacc ggctggttcc catcctgggc
     2581 gcgctggttt ccggcgatgc ccaggcgtat cgctaccttc caaactcgct gacgaccttt
     2641 cccgatgccc cctcgctcgc gcagatgatg cgggaggcgg gcttcagcca cgtgacctac
     2701 cggtacctct cccttggggc gatggctgcg cacgttgggg tgaagtaggg taatcgcctc
     2761 gaggtgcggc gcgttctttg cgttcttctg cggcgtgggt gatgcgcgac ggcccgtttg
     2821 accagggctc atgcgaattc gtattctgag agcgatggtg gtggtgttcg tctcgtaggg
     2881 gcgacgcatg cgtcgcccta accggcttcc ggtccgcctc acgtgattgc ggtcgcctgg
     2941 cctgtgatgc cgggatgcgg caacgcgtta tgggccgatt caaacaggat agacaggatg
     3001 gggacgaagg atttggaaaa ggtgaatgag taaacgcgaa atcgtcctgg gcaacctggt
     3061 cagcacggtg gccgtcgcat tgttggcggt aggctgtgcg tcctcatacg gtacccccgt
     3121 agctccgacc cagtctgcca catccacacc agccacactg gccggtgcgc cggcaacgac
     3181 cactcctaca gcgcaaccca aagtcacccc gatagccact gttggtgcga ctcaggtgcc
     3241 cgcccctgcg tctgcctcca cgcgagcccc cgtatccctg acccccgtgt ccgccggtgc
     3301 cgagccgacc attcaggtca cggggcaggt gcgtgacgtt gcggcaagcg ctcgagtgat
     3361 cacttttgta gaggccgtac acggcttcga ggacgtggct ctcacggaca gcacccagat
     3421 catcggcccc gacgggtcct cccggacgtt gcaggacatt cagcccggca tggtggttca
     3481 ggctgccgga tgggccaacg gttccggcgc ggtgatcgcc aagagcgtga agatcatctc
     3541 cggacctact ccacttccac agtaggctga cggcctttct ccttactctc cctcccctaa
     3601 tttcccattt gagatctacg caatccgctc gacccgccga ttattgttat tgaaccacgt
     3661 tgcagatgtg caatcacgag gggcgtgact ctgcccggtg catacgcagg acacggagac
     3721 tgccccgagg gtctgggctg gtgacgttgg agcctgttgt ctggcaacat actccctata
     3781 cagttctcct gatggtcgct gccggcgtcg cgatgctccg tgcgtggcag atctggcaga
     3841 gtcctatgtc cggagccagg ctcctggcca tgatcctgtt gttcgcgggg ctgtggagct
     3901 tcggatacgg cctcgagatg gccagcgcaa acctggctta caagcagctc tgggtgaaag
     3961 caaggtatat tgggatcaca gccgttccca ccttctgggc tctctttacc ctcaagtaca
     4021 ccggccgtaa tcgttggctg agcccgcgac cccttgggtt gctcctcctg gtacccatcg
     4081 ccaccttgat gattaccttt acggatagct ggcactccct catgtttgcc cgctatgaca
     4141 tgatctatgt ctccggtctt gccgttctgg agttggtcca cggcccctgg tttgtgttgt
     4201 ggaccgctta cgcctacggc cttctcctcc tgggaacgct gctgctgctg gagttgatgg
     4261 tcaggtctcg ccagctctat cactggcaga gcagtctgct gatctctgcc gcggccgtgc
     4321 cttgggcggt caacgtcata acggtgagcg acagcgcacg ggacgcgggg ttggatttct
     4381 catgcctggt ggtgattgcc acggcgatga tcgtcagttg ggggttggtc aggctacggc
     4441 tggacgacct ggtgccggtg gccaggcgca acttctttgc gaacacagct gacgcggtgt
     4501 tgcttctgga cgggcagggc agaatcctgg acgtcaatcc cagggcagag cagctgattg
     4561 gaaccaatgc ccgggcggtg atcggaaaga gcgtggggca ggtatggcca caatggcccc
     4621 agcgaatgga gcggctccag gagcagtcat cttcgagtga agagttgacg gtgggagagg
     4681 gcgaccagca gcggatctac gatgtcctcg agtcccccgt cgtcgattgg cgggggcgtg
     4741 tcgcttgctg gacggtggta cttcgcgaca taacccggcg tcgcaatgcc gaggatgcgc
     4801 ttcgggtgtc cgaggcgaag ctcctctcca tcctggactc ctcacccgat gccatcacag
     4861 tctgcgatct agacggcacc acgatcgact gcaaccaatc ggcactgaag atgatcggtc
     4921 tggagagcaa gactcagttg ttgggcgcca atatcctcga ctccgttcct ctccgtgaaa
     4981 ggggaagagc cggggagtcc atggggcaac tcctcaccga gggggtcctt cgtgacctgg
     5041 agttcgccat tgtgaccgtg gacggacggg aatttccggg ggagctgtcc gcaaggatca
     5101 tcgaagacga cagcggaagg gccacagcat tcgtcgttgt cacgcgcgac ataagcctgc
     5161 gcaagcagat gtcggatcag cttttgcgag ctcaacgtct cgagtctgcc ggccggattg
     5221 cagggcaggt ggcccacgac ttcaacaacc tcctatctcc cctatttgcc taccccgaac
     5281 tgatcaagaa gcagcttcca gaggggcatc ctgctttgct gtgttgcgat cagatgctgg
     5341 aggcggcgga gcgaatggcc tacataaacg aggagttgct gacgctggga cgtcgcggaa
     5401 acttcgtgct ggaacctacc gacctgaatt ccctggtgga gatcgccgtg gcagagatgg
     5461 gtgttcgccc gaatgggctt tgggtgcgga cgaagccgga gcctgagctc cggccggtta
     5521 tggcatcttc agcccagctg attcgggtga tctccaacct gctgtccaac gcccacgaag
     5581 cgatgccgaa cggtggggag atcacagttg cgactaagaa cgttcagctg ccggctccgt
     5641 tgagccccaa cgctcgggtc aagctggagg actacgtgcg gcttagtgtg accgacacgg
     5701 gttgtggaat acctcccgag atcgtggaca agatcttcga tgccttcttc agcaccaagc
     5761 ggagcgtcaa gagacgagga tgtggtttgg gactcagtgt agtgcagtcc atcgtggaag
     5821 accatcacgg ctacctggac ttgcagagcg aagtcggggt cggcaccacc ttccatgtct
     5881 acttgccggc aagcgacgag gcgcccaaga cgaggccgac ggaggttgtt aaagggggca
     5941 tagagaccat cctggtggtg gacgacgacc agcttcaacg tgatgtctcc gcggcgatgc
     6001 tgaacgagct gggataccgc gtggcggttg catccagcgg tgaggaagct ctggagtatc
     6061 tcaagggatc aacggtagac ctgctgctgc tggacatgat gatgcctccc gggatggacg
     6121 gggcagacac ctatcgtcgt gcgcgggaga ttcggcctgg attgaaagca gtcatcgttt
     6181 cggggtttgt cgccacggag cgggtgaggg aggcgcaatc gctgggcgcc ggacaatttc
     6241 tgcgaaagcc gctcaccctg gagaagcttg ccgaggccgt gcgtgtcgag ctagactgcc
     6301 gggatcagcc actggcgtca aattgagtat aggggattcc gcgaaggatg attgagaaga
     6361 tcgagactgc ctactatcgg ctcccgctgg agccgatggg cgatgcggga catggcgcca
     6421 tcgacgtgga ggagttcatc acgctcaagt tgtatgccga cggactggtg ggtcacggct
     6481 acgcctatac catcggccga ggcgggaggg ccatccagtc actcatcgac cacgacctgg
     6541 cgcccctgtt ggtggggcga gatgcggccg acgtcgaggg attgtgggac tacatgtggc
     6601 accgtctcct gtacgtcgga cggggcgggg tggcgagctt tgccatcgcg gccgttgaca
     6661 tcgccctctg ggaccttcgg ggcctccggg agtgcaagcc actctatgct ctggctgggg
     6721 gaactgcccg cgagatcccc gcgtatggct ccggcgtgga cctgccgaag ccgaaggacg
     6781 aactgctgag ccaggtagag ggcttcctga agcgtggttt ccccggtgtc aaggtcaaga
     6841 tcggccgacc ggacccgcgg gaggatgagg atcgcgtgga ggccgtgcgg aagctgatcg
     6901 gggacggcgt ggatctgatg atcgatgcca acatgacctg gccggcggag gtggcgttgg
     6961 agcggggcaa gcggttcgag ccgttcaatc ccttctggtt cgaggaaccc accataccgg
     7021 aggatgtccc gggccatgca cggctcgtgg aggggttggt gacacccatc gcgctgggag
     7081 aaagcctgca ttcgccatac gagtatcagc gctacgtcga gaccagggcg gtggaagtgt
     7141 tgcagatcga tcccgtcacc aacggcggca ttacctcgtc cctgaaggcg ttggcgatgg
     7201 ccgacgcggc gggtctgaag accagcagcc actacaccga tgagttgtgc gcccacctgc
     7261 tgtgcgccag ccgaaacccc atatacctcg agaagcacgc cttcgcgctg gatccctatc
     7321 tgctgcagcc gcagcaggtt gtgaacggca gggtccgccc cacggaggtc cccggcaccg
     7381 gcatgcgctt cgacgagaag gcgatggcgc ccttccgcgc ctagccctaa ctcggttcac
     7441 gtgtgggcga tgcctccatc gcccacacgg ttctcctcaa ggccctcatc ctgcatttct
     7501 tcgcccaaac cgtgcgccgg aggtttgggc aggacatggc ggatgggtcg ctaacacgac
     7561 gtcttccggt gggcgctgag ttgcagccgg aaggcgccgt tcactttcgg gtttgggccc
     7621 cgggacgcga taccgtagag ctggtactgg aatcgggtcc aggcgagggg agccgctgca
     7681 tcggcatggc gccggaagga aacggctact tccagctcac cgtcccgggc gcggtcgcgg
     7741 gcaccctcta ccgttacctc ctagacgggg aaggacccta ccccgatccg gcatcacgct
     7801 ttcagccact
//
LOCUS       DATALS010000480         3817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_444698, whole genome shotgun sequence.
ACCESSION   DATALS010000480 DATALS010000000
VERSION     DATALS010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444698"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..739)
                     /locus_tag="VHS06_10720"
     CDS             complement(5..739)
                     /locus_tag="VHS06_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEX2988628.1"
                     /translation="MSLDPRSRLISTLFLMFPLLLTPSWAELGVYVLILLLGMWLTGE
                     FKAVSRAIFPVTLLAFGIAAFSALASPGERTLLGGLLPISVDGLEVGTKSGIRMVELV
                     ALGALLAATTPPAKLAAALGWIISPMRHLKVDVPSLEFVVVLVFQFVPILKSELAATE
                     TAMYLRGARYTRNPIDQIRRLSVLPVPLLRRLLVATDEMAIALLSRGYRLDKRMSGAD
                     MRFRRVDLLAMGGSAILAICVIAASL"
     gene            complement(748..1611)
                     /locus_tag="VHS06_10725"
     CDS             complement(748..1611)
                     /locus_tag="VHS06_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988629.1"
                     /translation="MQLELRNVSYRYAATDDRLALEDVSLRFHTGEAVLVLGHSGAGK
                     STLLHLMAGLLKPSSGQVVFDDGVRNGVCTSLALQQPERQLFCGSVFDEVAFGLRQTY
                     RSKRMALERRSREDFVRQQVESALLAVGLDPQAYVSRHPQQLSDGEKRRLAIACCLAT
                     KPRFLLLDEPLAGLDAPGRRALLRTLGSLRSNGTGVVVTSHSVEPLLPIVDRILLFRE
                     GRVVGDGSPSNILGDLSLLSACGVEPPGIASVARRLQERGWPALEWSTPDRLADSILS
                     CLRHPDGREVP"
     gene            complement(1587..2414)
                     /locus_tag="VHS06_10730"
     CDS             complement(1587..2414)
                     /locus_tag="VHS06_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2988630.1"
                     /translation="MLSCRSVAFRFDDQSPWVLNNLDLTLAPGEFCTILGHNGCGKSS
                     LCRLLSGIYLPSTGSVELDGVGVGALVESGRLPTLIHLVLHDPHWQIIGDSVSQDIEL
                     GLRNVGLSGQALMDRVDWSLSLLDIDAYRHRSVHGLSGGELQLVRLAGAIAVQPRFLI
                     LDEALAMIDGARRRRILGTLRDLTKELRMSVLLTTHDVEDIALADRVLWLRSGRIAMD
                     EPCDTALQSLVDTIPQPFDIPGVASLAVRLRGAGRPVRLTLDVEQLVDDICSLNCET"
     gene            complement(2422..2997)
                     /locus_tag="VHS06_10735"
     CDS             complement(2422..2997)
                     /locus_tag="VHS06_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX2988631.1"
                     /translation="MYVRMRNLTSVALFAALTVALGAVYIPLPWSPIPITGQTLGAML
                     AGGVLGARRGLQSMLLVMVLVAVGLPVLAGGRGGLGVFVGPTAGYLIAWPIVALFIGW
                     AVERIPFGKASLPLLMATNIAGVFLIYVPGVLWMSYVTSVPIHQAVVAGVLPFIPGDL
                     VKAFAATMATFALWRTSDPRAIARTALATHQ"
     gene            complement(3265..>3817)
                     /locus_tag="VHS06_10740"
     CDS             complement(3265..>3817)
                     /locus_tag="VHS06_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX2988632.1"
                     /translation="VAQSRPRKQLMAATKSNACQYSMVFWDEMLAKVGEEFPDVKASK
                     CHVDALAAQFVLRPEALDVVVASNLFGDILTDLGGALQGGLGIPASANLNPEKRYPSM
                     FEPVHGSAPDIAGRGLANPIAAIWAGASMLEFLGEKSAADAVLSAMEAVTAEGKVVTP
                     DLGGRASTDEMAAEIAAKLRANA"
BASE COUNT          841 a         1213 c         1082 g          681 t
ORIGIN      
        1 cacgctatag agaagccgcg attacacaga tcgctagaat ggcgctgcct cccatggcca
       61 ggaggtccac acgccggaac cgcatgtcag ccccactcat acgcttatcc agtcgatagc
      121 ctcgagacag gagggcaatc gccatctcgt ctgtagcaac aagcaggcga cggagaagtg
      181 gaacaggcag gaccgataga cgccgaatct gatcgattgg attcctggtg tacctcgcac
      241 cacgcaaata catcgccgtc tcggtcgccg caagctccga cttgaggatc ggaacgaact
      301 gaaagaccaa cactaccacg aactccagcg atggtacgtc taccttgaga tggcgcatgg
      361 gagagattat ccaaccgagc gcggctgcca gtttggcagg tggggtggta gccgcgagca
      421 gagcccctag tgcgaccagt tcaaccatcc ggatcccaga ctttgttcca acctcgagac
      481 catcgacgct gattggcagc aaccctccca gcagcgtcct ctcgcctggt gaggctaatg
      541 ccgagaaggc ggcaatgcca aaagcaagca gggtgacagg gaagatggcc cgactcacgg
      601 ccttgaactc tccagtcaac cacattccca acagcagaat cagtacgtag acccccagtt
      661 ccgcccacga gggtgtgagc aggagcggaa acatcagaaa cagggtcgag atcagtctgg
      721 atcgtggatc aagcgacatg cgacctccta ggggacttcg cgtccgtctg gatgacgcag
      781 gcaactgagt atcgagtcgg ccagcctatc cggggtcgac cattccaggg ccggccaacc
      841 gcgctcttgg agacgtcttg cgaccgacgc gatcccgggc ggctcgacgc cacacgcgga
      901 cagcagtgag aggtcgccaa gaatgttgct ggggctgccg tcaccgacca cgcgcccttc
      961 tcggaagagg aggatcctat ctacgatggg caagagcggc tcgacagaat gacttgtcac
     1021 gaccacgcct gttccgtttg agcgaagact accaagtgtc cgaagcagcg cacgacgtcc
     1081 aggggcgtcc aggccggcaa ggggctcgtc caacaacagg aagcgcggtt tcgtcgccag
     1141 gcagcacgct atcgcgagtc gtcgcttttc gccgtcacta agctgctgcg gatgcctgga
     1201 gacatatgcc tggggatcga ggccgactgc caggagtgcc gactcaacct gttgccttac
     1261 gaagtcctct cgagatcggc gctccaaagc catccttttc gatctgtagg tttggcgtaa
     1321 tccgaaagcg acttcgtcga atacgcttcc gcagaagagt tgacgttccg gctgttgaag
     1381 tgccaggctg gtgcaaaccc cgtttcgaac gccatcatcg aacacaacct ggccggacga
     1441 cggcttcagc agtccggcca tcagatgcaa cagtgtcgac ttcccagcgc cgctatgtcc
     1501 aagaaccagg acagcttcac ccgtgtggaa cctgagagag acatcttcca gggcgagtct
     1561 gtcgtcggta gcggcatacc gatagcttac gtttcgcagt tcaagctgca tatgtcatcg
     1621 accaattgct cgacgtcgag agtaagtctc accggcctgc cggcgccacg cagacgaact
     1681 gccagtgatg caacaccagg gatatcgaag ggctgaggga tagtgtcaac cagcgactgc
     1741 agggcggtat cgcacggctc gtccattgcg atcctcccag atcggagcca gagcaccctg
     1801 tcggccaggg cgatgtcttc gacgtcgtga gtggtcaaca aaacagacat ccggagttcc
     1861 ttcgtgagat cgcgaagtgt gccgagaatg cgacgccgtc tggcaccgtc tatcatcgcc
     1921 agagcctcgt ccaatatgag gaaacgtggc tgcactgcta tcgcaccggc cagccttacg
     1981 agttggagtt cgcctccaga aagaccgtgg accgagcggt gtctgtaagc atcgatatcc
     2041 agcagggaca aagaccaatc gacgcgatcc atcaacgctt ggccggataa tcccacgttc
     2101 cgcaatccaa gctcgatatc ctggctcacc gaatccccaa tgatctgcca gtgcggatca
     2161 tgcaggacga ggtggatgag cgtcggtagc ctcccgctct ccacgagtgc gccaacgccg
     2221 actccgtcca actcgacact accggtactc ggcagataga tgccgctcag cagcctgcac
     2281 agcgaggact ttccgcagcc gttgtggccc aggatcgtgc agaattcgcc gggggcaagg
     2341 gtgaggtcta gattgttcag aacccagggt gactgatcgt cgaacctgaa ggcaaccgat
     2401 cggcagctta acattgaccg gctactgatg tgtcgccagg gccgttcggg ctattgccct
     2461 tggatcactc gttcgccaca gggcgaatgt cgccatggtg gcggcaaatg ccttaaccag
     2521 atccccgggt atgaagggta gcactcccgc cacaacagcc tgatggatgg ggacgctcgt
     2581 cacgtagctc atccacagaa cacccggcac gtagatgagg aagaccccgg cgatattcgt
     2641 tgccattaga agcggaagcg acgccttgcc aaagggaatg cgttccaccg cccacccgat
     2701 gaacagggcc actatcggcc acgcaatgag atatccggcg gttggaccta cgaagacacc
     2761 aagtccccct cgtccccccg caagcaccgg cagccctacg gcaaccagga ccatcaccaa
     2821 gagcatactc tgtagccctc tgcgagcacc gagtacgcct ccggccagca tcgctccaag
     2881 tgtctgtccg gtaatcggaa tgggcgacca gggtaatgga atatagactg cgcccagcgc
     2941 caccgtcagc gcggcgaaca gcgccaccga cgtcagattg cgcatcctga catacatagg
     3001 attcaacaac gcagtgcctc caatgatagt caaccgcaag gcagggtagt gcaatggaac
     3061 ggcaggcgtc aagctataca catgctcgtt ccgtctctca ccattcatga gaacagcctc
     3121 cgcagtcgct cggctttctt ccagacccaa gctctacaga ggctgttcct cttgcctatt
     3181 cacttccgcc ccagaattcg gggtgtgtcg cgatcatcag gttgaggcag cccgcctcac
     3241 caatgagacg ttgaccgagg ccggttaggc atttgctcgc agcttcgccg cgatctccgc
     3301 agccatctca tcggtgctgg cacgcccgcc caggtcgggt gtcaccacct taccctcagc
     3361 cgtcaccgcc tccatcgcac tcagcaccgc atccgccgcg gatttctctc cgaggaactc
     3421 cagcatcgac gcaccggccc agattgccgc gatggggttg gcgagccccc tgcccgcgat
     3481 gtccggggcc gatccgtgaa ccggctcgaa catggagggg tagcgcttct ccggattgag
     3541 gttcgcgctg gcaggaatgc cgagcccgcc ctgcagcgcg ccgcccaggt cggtcaggat
     3601 atccccgaag aggttggacg ccaccaccac gtccagcgcc tcaggtcgga gaacgaactg
     3661 cgccgccagt gcgtccacgt ggcacttgga tgccttgaca tcggggaact cctcccccac
     3721 cttcgccagc atctcatccc agaacaccat gctgtactgg caggcgttgg acttggtcgc
     3781 ggccatgagc tgcttccgcg gccggctctg cgccacc
//
LOCUS       DATALS010000481         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_444704, whole genome shotgun sequence.
ACCESSION   DATALS010000481 DATALS010000000
VERSION     DATALS010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444704"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VHS06_10745"
     CDS             complement(<1..802)
                     /locus_tag="VHS06_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988633.1"
                     /translation="MQLELRNVSYRYAATDDRLALEDASLRLHTGEAVLVLGHSGAGK
                     STLLHLMAGLLKPSSGKVVFGDGVRNGVCTSLALQQPERQLFCGSVFDEVAFGLRQTY
                     RSKRMALGRRSQADFERQQVESVLLAVGLDPQAYASRHPQQLSAGEKRRLAIACCLAM
                     KPCFLLLDEPLAGLDAPGRRALLRTLGSLRSNGTGVVVTSHSVEPLLPIVDRILLFRE
                     GRVVGDGSPSNILGDLSLLSACGVEPLGIASVAKRLQERGWPALEWSTP"
     gene            complement(778..1605)
                     /locus_tag="VHS06_10750"
     CDS             complement(778..1605)
                     /locus_tag="VHS06_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2988634.1"
                     /translation="MLSCRSVAFRFDAQSPWVLNNLDLTLAPGEFCTMLGHNGCGKSS
                     LCRLLSGIYLPSTGSIELDGVGVGALVESGRLPTLVHLVLHDPHWQIIGDSVSEDIEL
                     GLRNVGLSGQALMDRVDWSLSLLDIDAYRHRSVHGLSGGELQLVRLAGAIAVQPRFLI
                     LDEALAMIDGARRRRILGTLRALTKELQISVLLTSHDVEDIVLADRVLWLQSGRIAMD
                     EPCDTALQSLVDAIPQPFDIPAVASLAVRLRGAGEPVSLTLDVEQLVDDICSLNCET"
     gene            complement(1613..2200)
                     /locus_tag="VHS06_10755"
     CDS             complement(1613..2200)
                     /locus_tag="VHS06_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX2988635.1"
                     /translation="MLNPVYVRMRNLTSVALFAALTVALGGVYIPLPWSPIPITGQTL
                     GAMLAVGVLGARRGLQSMLVVMVLVAVGLPVLAGGRGGLGVFVGPTAGYLIAWPIVAL
                     FIGWVVERIPFGKSSLPLLMATNIAGAFLIYVPGVLWMSYVTSVPIQQAVVAGVIPFI
                     PGDMVKALAATMATFALWRTSDPRATARTALATHL"
     gene            complement(2461..>3013)
                     /locus_tag="VHS06_10760"
     CDS             complement(2461..>3013)
                     /locus_tag="VHS06_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX2988636.1"
                     /translation="VAQSRPRKQLMAATKSNACQYSMVFWDEMLAKVGEEFPDVKASK
                     CHVDALAAQFVLRPEALDVVVASNLFGDILTDLGGALQGGLGIPASANLNPEKRYPSM
                     FEPVHGSAPDIAGRGLANPIAAIWAGASMLEFLGEKSAADAVLRAMEAVTAEGKVVTP
                     DLGGRASTGEMAAEIAAKLRASA"
BASE COUNT          637 a          975 c          859 g          542 t
ORIGIN      
        1 ctggggtcga ccattccaga gccggccagc cgcgttcttg gaggcgtttt gcgaccgacg
       61 cgatcccgag aggctcaaca ccacatgcag acagcagcga gaggtcgcca aggatgttgc
      121 tagggctgcc gtcaccgacc acgcgccctt ctcggaagag gaggatccta tctacgatgg
      181 gcaagagcgg ctcgacagaa tggcttgtca cgaccacgcc cgttccgttt gagcgaagac
      241 taccaagtgt ccgaagcagc gcacgacgtc caggggcgtc cagaccggca aggggctcgt
      301 ccaacaacag gaaacacggt ttcatcgcca agcagcacgc tatcgcgagt cgtcgctttt
      361 cgccggcgct gagctgctgt gggtgtctgg aggcataggc ctggggatcg agaccgactg
      421 ccaggagcac agactcaacc tgttgccttt cgaagtccgc ttgagatcga cgcccgagag
      481 ccatccgttt cgatctgtag gtttggcgta atccgaaagc gacttcgtcg aacacgcttc
      541 cgcagaagag ttgacgttcc ggctgttgaa gcgccaggct ggtgcaaacc ccgtttcgaa
      601 cgccatcacc gaacacaacc ttgccggacg acggtttcag cagtccggcc atcaggtgca
      661 acagtgtcga cttcccagcg ccgctgtgtc ctagaaccag gacagcttca ccggtatgga
      721 gcctgagaga ggcatcttcc agggcaagtc tgtcgtcggt agcggcatat cgatagctta
      781 cgtttcgcag ttcaagctgc atatatcatc gaccaattgc tcgacgtcga gagtaagact
      841 cacaggctcg ccggcgccac gcagacgaac tgccagcgat gcaacagctg ggatatcgaa
      901 gggctgaggg atagcgtcaa ccagcgattg aagggcggta tcgcacggct cgtccattgc
      961 gatcctccca gattggagcc agagcaccct gtcggccagg acgatgtctt cgacgtcgtg
     1021 actggtcagc aagactgaaa tctggagttc cttcgtgaga gcgcgaagtg tgccgagaat
     1081 gcgacgccgt ctggcaccgt ctatcatcgc cagagcctcg tccaatatca ggaaacgtgg
     1141 ctgcactgct atcgcaccgg ccagcctcac aagctggagt tcgcctccag aaagaccgtg
     1201 gaccgagcgg tgtctgtaag catcgatatc cagcagggac aaagaccaat cgacgcgatc
     1261 catcaacgct tggccggata atcccacgtt ccgcaatcca agctcgatat cctcgctcac
     1321 cgaatcccca atgatctgcc agtgcggatc gtgcagcacg aggtggacga gcgtcggtag
     1381 cctcccgctc tccacgagtg cgccaacgcc gactccgtcc aactcgatgc taccggtact
     1441 cggcagatag atgccgctca ggagcctgca cagcgaggat tttccgcagc cgttgtggcc
     1501 cagcatcgtg cagaattcgc cgggggcaag ggtgaggtct agattgttca gaacccaggg
     1561 cgactgagcg tcgaacctga aggcaaccga ccggcagctc agcatcgatc gactacagat
     1621 gtgtcgccag tgctgtccgt gctgttgccc tcggatcact cgttcgccac agggcaaacg
     1681 ttgccatagt ggcagcaagt gccttgacca tgtccccggg tatgaagggt atcactcccg
     1741 ccacgacagc ctgctggatg gggacgctcg tcacgtagct catccacaga acgcccggca
     1801 cgtagatgag gaaggctccg gcgatgttcg ttgccatcag gagcggaagc gacgacttac
     1861 cgaaggggat gcgttccacc acccacccga tgaacagggc cactatcggc catgcaatga
     1921 gatatccggc agttggacca acgaagaccc caagtccccc tcgtcccccc gcgagcaccg
     1981 gcagccctac ggcaaccaga accatcacca cgagcatact ctgtaaccct cttcgagcac
     2041 cgagcacgcc tacggccagc atcgctccaa gtgtctgtcc ggtaatcggg atgggcgacc
     2101 agggcaaggg aatatagact ccacccagcg ccaccgtcag cgcggcgaac aacgccaccg
     2161 acgtcagatt gcgcatcctg acatacacag gattcaacaa cgcagtgcct ccaacgatag
     2221 tcaaccggaa ggcagggtag tgcaatggaa cggcaggcgt caagctatac acatgctcat
     2281 tccgtctctc accattcatg agaacagcct ccgcagtcgc tcggcttcct tccagaccca
     2341 agctctacag aggctgttcc tctcttctta ccgattcact tccgccccag gattcgaggc
     2401 gtgtcgcgat catcaggttg aggtagcccg cctcaccaat gagacgttga tcgaggccgg
     2461 ttaggcactt gctcgcagct tcgccgcgat ctccgcggcc atctcgccgg tgctggcacg
     2521 cccgcccaag tcgggtgtca ccaccttacc ctcggccgtc accgcctcca tcgcacgcag
     2581 cactgcatcg gccgcggatt tctctccgag gaactccagc atcgacgcac cggcccagat
     2641 tgccgcgatg gggttagcga gccccctgcc cgcgatgtcc ggggccgatc cgtgaaccgg
     2701 ctcgaacatg gaggggtagc gcttctccgg attgaggttc gcgctggccg ggatgcccag
     2761 cccgccctgc agcgcgccgc ccaggtcggt caggatatcc ccgaagaggt tggacgccac
     2821 cacgacatcc agtgcctcag gccggaggac gaactgcgcc gccagtgcgt ctacgtggca
     2881 cttggatgcc ttgacatcgg ggaactcctc ccccaccttc gccagcatct catcccagaa
     2941 caccatgctg tactggcagg cgttggactt ggtcgcggcc atgagctgct tccgcggccg
     3001 gctctgcgcc acc
//
LOCUS       DATALS010000482         4920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_353196, whole genome shotgun sequence.
ACCESSION   DATALS010000482 DATALS010000000
VERSION     DATALS010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353196"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VHS06_10765"
     CDS             complement(<1..464)
                     /locus_tag="VHS06_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HEX2988637.1"
                     /translation="MTLSMELSVETAVAKARDLFDGGYSCAESTLQVLLEMYGLEDDG
                     VWTAAAGFGGGVGRMQSVCGALAGGTIALGLLAGRKAGNRAETAVIVRPAVQRLVEGF
                     ESRFGNMNCGDLVVPFNFRKQGEYDRFKATNLRQERCHHYVKYVVEALASVP"
     gene            complement(621..1460)
                     /gene="larE"
                     /locus_tag="VHS06_10770"
     CDS             complement(621..1460)
                     /gene="larE"
                     /locus_tag="VHS06_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX2988638.1"
                     /translation="MDELRAKHEQLKGILRSMGSVAVAYSGGVDSTFLLRVAVDELGM
                     DKVLGIIAVSESYPARELEEAKQLADEMGARIRVIHTDELSNPEYAKNPADRCAICKT
                     ELFGKMFPIAKEEGLQFLVYGVNADDMGDFRPGIAAAKRMGARGPLQEAGLTKAEIRQ
                     LSRELGLRTWDKPAFACLSSRIPYGQRITREALLQVDAAENFIRDLGFRQVRVRHHDK
                     IARIELPPDQIPQLFAGGFADLVAAKLKELGYLYVTVDIQGYRVGSLNEALRITVPGK
                     TAQ"
     gene            complement(1519..2580)
                     /gene="mnmA"
                     /locus_tag="VHS06_10775"
     CDS             complement(1519..2580)
                     /gene="mnmA"
                     /locus_tag="VHS06_10775"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX2988639.1"
                     /translation="MSGGVDSSVAAALLKQQGYDLIGVTLNVWPEQSLVEADTRRSAC
                     CSLDAVDGARRVADLLDFPHYTLNFREIFDRNVIRDFVSEYAAGRTPNPCVRCNRFVK
                     FEALLDKARSLGAEFLATGHYARIGRSSEGRYVLGRAVDDSKDQSYALYSLTQEQLAH
                     TLFPLGGQTKKETRRIAAEMGLATADKPESQEICFVQDNDYTGFLRARDPSVVKPGPI
                     KDRDGHVLGSHSGIAFYTVGQRKGLGIATGKPLYVVEIVPEENAIVVGELESVYSHGL
                     VAGDLNWVSTAECQEPLRVEAKIRYRATAVPATACVVEVGRLRVEFDDPQRAVTPGQA
                     VVLYNGDDVVVGGTILRRH"
     gene            complement(2799..3752)
                     /gene="cysK"
                     /locus_tag="VHS06_10780"
     CDS             complement(2799..3752)
                     /gene="cysK"
                     /locus_tag="VHS06_10780"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013324158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX2988640.1"
                     /translation="MKIANDVTELIGNTPLVRLNRVTEGCVATVVAKLESFNPGGSAK
                     DRIGVSMISEAEKQGLIVPGKSVLVEPTSGNTGIALAWVAAARGYRLILTMPETFSVE
                     RRLLFAALGAEVVLTPGPEGMRGAILKAEELVKKIPYAWMPQQFENPANPKIHRETTA
                     EEIWRDTEGKADFVVAGVGTGGTITGIAQVIKQRKPEFKAIAVEPAASPVLEGGAAGP
                     HKIQGIGANFVPKVLDMSVVDEVIPVENDDSFDMARQLARREGILCGISSGAATWAAV
                     QVAKRPENAGKLIVVILPDTGERYLSTPLFQEPQPSTPPSI"
     gene            complement(3866..4315)
                     /locus_tag="VHS06_10785"
     CDS             complement(3866..4315)
                     /locus_tag="VHS06_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX2988641.1"
                     /translation="MDQEAQRSIHLPASYYREIVAHAREGKPNEICGLIAGKDGYPVK
                     LYRTTNNDPRPRVRYNVEPMELLQVLREIEENGWALHAIYHSHPMSEAYPSGTDTSLA
                     YYPDAIYLICSLEDDRSPVVRAFNIVDGEVTELILEIDEEMERSTVL"
     gene            complement(4387..4812)
                     /locus_tag="VHS06_10790"
     CDS             complement(4387..4812)
                     /locus_tag="VHS06_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX2988642.1"
                     /translation="MKSEYGLRAMMELALAYGGRPLQTADIAARRAIPESYLEQLLTT
                     LRKAGFVSSVRGPQGGHTLAVHPSTITAGDVVRALEGPVVVVDAMEGTTAGQSTSPTV
                     LRELWGRVRTAIDSALDGVTVEELAARQAAEEGSINYSI"
BASE COUNT          914 a         1669 c         1400 g          937 t
ORIGIN      
        1 cttggaacgg aggccagtgc ctccaccacg tactttacgt agtggtgaca ccgctcctgc
       61 ctcagattgg tcgccttgaa gcggtcgtac tcaccctgct tccggaaatt gaagggcacc
      121 accaggtcac cgcagttcat gttgccgaaa cgagactcga acccctcgac caatcgctgc
      181 accgccggac gaacaataac ggccgtctca gcccggttcc ctgccttacg gccggccagc
      241 aatcccaacg ctatagtgcc gccggcaagg gctccgcaaa cagactgcat acggcccact
      301 ccacccccga acccggcggc agcggtccat acgccgtcgt cttcgagacc gtacatctcc
      361 agaagtacct gtagggtcga ctccgcgcag ctatatccgc cgtcgaaaag gtcccgcgcc
      421 ttggccaccg ctgtctccac tgacagctcc atggacaacg tcatccggtg aacctcccac
      481 caacgctggt tagagtaaac ggcgcctatg ctaacatacc ggcaccccgg gccgcatgcc
      541 gcacgagcga caacaaagaa ggcctcgctc gattgcgagg cctctagcta tgacgtagat
      601 gaagggggaa gtggcgcgag ctactgggcc gtcttgccgg gcaccgtgat ccgcagcgcc
      661 tcgttgaggc taccgacacg gtagccctgg atatccacgg tgacgtagag gtagcccaat
      721 tccttcagct tcgcggcgac gagatcggcg aagccacctg cgaacagctg cgggatctga
      781 tcgggcggca gctcgatccg ggcgatcttg tcgtgatggc gaacccggac ctggcggaac
      841 cccagatccc ggatgaagtt ctccgccgca tccacctgca gcaacgcctc tcgagtgatc
      901 cgctgcccgt acgggatcct tgaggagagg caggcgaagg caggcttgtc ccaggtgcgc
      961 agccccagct ccctggaaag ctgccggatc tcagccttgg ttagaccggc ctcctgcagc
     1021 ggtcccctcg cccccatccg cttcgccgca gcgatgcccg ggcggaagtc gcccatgtcg
     1081 tcggcattga cgccgtacac cagaaactga aggccctcct ccttcgctat ggggaacatc
     1141 ttgccgaaga gctccgtctt gcagatggca cagcgatcgg ccgggttctt agcgtattcg
     1201 ggattggaga gctcgtcggt gtgtatcacc cggatccgag cccccatctc gtcggccaac
     1261 tgcttggcct cctctaattc ccgcgccgga taggactccg agaccgcaat gatgcccagc
     1321 accttatcca tgcctaactc atctacagcc actctgagca ggaaggtgct gtccacccca
     1381 ccggagtaag ctactgccac gctccccatg gagcggagaa tccccttgag ctgttcgtgc
     1441 ttcgcgcgca gttcgtccat ctgtgacctc atctagctat cagcggtcag tcatcggcta
     1501 tcagcagtcg gcgatcagct aatggcggcg cagaatcgtc ccgcccacca caacgtcgtc
     1561 gccattgtac agcacgaccg cttgccccgg tgttaccgcc ctctgtgggt cgtcgaactc
     1621 caccctcagg cgaccgacct cgacgacgca ggcagtcgcg ggcaccgctg tcgccctgta
     1681 gcggatcttc gcctcgaccc gcagcggttc ctggcactcc gccgtcgaca cccagttcag
     1741 atcgcctgcg accagaccgt gcgagtatac cgactccagc tcgcccacca cgatggcgtt
     1801 ctcttcagga acgatttcga ccacgtagag cggctttccg gtggcgatcc cgagcccctt
     1861 ccgctgccca acagtgtaga aggcaatccc ggaatggctc cccaacacgt gaccgtcgcg
     1921 gtccttgatg gggccgggtt tcacgaccga cgggtctcga gcgcgcagaa agcccgtgta
     1981 gtcgttgtcc tgaacgaagc agatctcctg actctccggc ttgtcggccg tggcaagccc
     2041 catctccgcc gcgatccgtc gggtctcctt cttggtctgc ccgcccaacg gaaacagtgt
     2101 gtgggccagc tgctcttgcg tcagcgagta gagggcgtag gactggtcct tggagtcgtc
     2161 caccgcccga ccgaggacat agcggccctc cgaggaccga ccgatccgcg cgtagtggcc
     2221 cgtggcaagg aattccgccc ccagggatct cgccttgtcc agcaacgcct cgaacttgac
     2281 gaagcgattg cagcggacgc atgggttggg cgttcgaccc gcggcgtact ccgatacgaa
     2341 atcccggatg acgttgcggt cgaagatctc gcggaagttc agcgtgtagt gtgggaaatc
     2401 cagcaggtcg gcgacccggc gcgcaccatc gacggcatcc agtgaacagc aggcgctacg
     2461 acgggtgtcc gcctcgacca gcgactgttc gggccagacg ttcagagtca ccccgatcag
     2521 atcgtacccc tgctgcttca acagagccgc ggcaacggag ctgtccaccc cgccgctcat
     2581 ggccaccacg acaagtggag acaagctgtt ccccctcaac gcgaattact cagtattatt
     2641 ccgcgagaat ctaattatag ttgctgagcc catccctgga caaggccggt cctgcggtgt
     2701 tggggcgagg catacctcgc ctttgtggaa tgcggacacc aggtgcggag tcaccgtttt
     2761 tgagtgcggc ggcaagccgc cgcactccag aaaagcccct agatggacgg cggagtgctg
     2821 ggctgcggct cctggaacag cggtgtgctg agataccgct cgccggtatc gggcaggatc
     2881 accacgatca acttgcccgc gttttccggg cgcttggcca cctggacagc ggcccaggtg
     2941 gcagcaccag aggagattcc acagaggatg ccctcccgcc ttgccagctg tcgggccatg
     3001 tcgaaggaat cgtcgttctc gacagggatg acctcgtcca ccacgctcat gtccagcacc
     3061 ttgggaacga agttggcacc aatcccctgg atcttgtggg gcccggcagc cccaccttcc
     3121 agcaccgggg acgccgcggg ctccaccgcg atcgccttga actcaggctt gcgctgcttg
     3181 atcacctggg ctatgccggt aatggtcccg ccggtcccga ccccggccac gacgaagtcc
     3241 gccttcccct cggtgtctct ccagatctcc tccgccgtcg tctccctgtg gatcttcggg
     3301 ttggcagggt tctcgaactg ctgcggcatc cacgcgtaag ggatcttctt caccaactct
     3361 tcagccttga gaatggcgcc cctcatcccc tcggggccgg gggtcagcac gacctccgcg
     3421 cccaacgccg cgaacagcaa ccgacgctca acgctgaagg tctccggcat cgtgaggatc
     3481 agcctgtagc ctcgggccgc cgcaacccac gccagggcaa tgccggtgtt tccgctggtc
     3541 ggctccacca gcacgctctt ccccggcaca atcagcccct gcttctcggc ctcactgatc
     3601 atgctcaccc caatccggtc cttggcacta ccccctgggt tgaagctttc cagcttggcg
     3661 acgacggtgg cgacacatcc ctcggtaacc cggttaagtc ggaccagcgg cgtgttgccg
     3721 atcagttcgg ttacatcatt ggcgatcttc attagaccct ctcctccagc gccatgtctc
     3781 gcatccaatg tgctgctaag cggctttccc tagatgcacc aacctttcca tgagtggaaa
     3841 gtggatacca acaagggaaa gtcgcttaga gcaccgtgga gcgctccatc tcctcgtcga
     3901 tctccagaat caactcggtc acctcgccgt ccacaatgtt gaatgccctc actaccggcg
     3961 atctgtcgtc ctcaagagaa cagatgaggt agatggcatc cgggtagtaa gccaggctgg
     4021 tatcggtgcc cgatgggtac gcctcgctca tggggtgaga atgatagatg gcatgcagcg
     4081 cccagccgtt ctcctcgatc tctctcaaga cctgcagcag ttccatcggc tcgacgttgt
     4141 agcggacccg cggcctggga tcgttgtttg tcgttcggta cagcttgacc ggatatccgt
     4201 ccttgcccgc gatcagtccg cagatctcgt tgggctttcc ttctctagcg tgggcgacga
     4261 tctcccgata gtaactggcc ggcaggtgaa tcgacctctg cgcttcctga tccactggtg
     4321 actcctctct ggggttttcc tccactccat gcagatcgtg cgccaggaac cggcaagtac
     4381 tcgccgctag atgctgtagt tgatggagcc ctcttcggca gcctggcggg ccgccaactc
     4441 ttcgacggta acgccatcca gagccgagtc gatcgcagtg cgcaccctcc cccacagctc
     4501 tcgcagaacg gtggggctgg tagactgacc ggccgttgtg ccctccatcg cgtccacgac
     4561 aaccacgggg ccctccaacg ccctcacgac atcccctgcg gtaatggtgg aggggtgaac
     4621 cgcaagggta tgtcccccct gcggccctct gacgctggag acgaatcccg ccttgcgcag
     4681 ggtggtcaga agctgctcca ggtacgactc cgggatggca cggcgcgcgg cgatatccgc
     4741 ggtctgcagc ggcctgccac cgtaggcaag ggcaagctcc atcatggcgc gcagcccgta
     4801 ttcgctcttc atcgagatcc gcacggtctg catggagtgg aggaaaaccc cagagaggag
     4861 tcaccagtgg atcagcaagt gcaaaagtcg attcacctgc cggccagtta ctatcgggag
//
LOCUS       DATALS010000483         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_354200, whole genome shotgun sequence.
ACCESSION   DATALS010000483 DATALS010000000
VERSION     DATALS010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354200"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VHS06_10795"
     CDS             <1..1081
                     /locus_tag="VHS06_10795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX2988643.1"
                     /translation="RLLSEVQRRVAELDTIIGSIADAVVVFGPDMKTILMNRAAERML
                     GYSLEQYRLEPEKRVQLLRMETPEGEPFPLEEVPSLRAARGETVSGVIMVFHPPGRTI
                     WVSVSSGPIRTPDGRIWGSVVSMTDITELHRLQEQREDLVRMVSHDLRSPLTSVQSQA
                     QIIQRLLQRTGGDGQLRQSVDAIYTGARRMNAMIQDLVDMARVESGQLTLNRTRLELR
                     SYLAELIQRLKGVLDVERVRLEVEEGLPEVIADPDRLERVMTNLLSNALKYSLPETPV
                     RVTATCIDGNVQLSVKDEGVGIAPEDLRRLFERFYRASGARKTEGLGLGLYISRMLVE
                     AMGGKIWVESEIGQGSSFNFTLLLA"
     gene            1441..2004
                     /locus_tag="VHS06_10800"
     CDS             1441..2004
                     /locus_tag="VHS06_10800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2988644.1"
                     /translation="MSTESPQIQRMIARLTVGSFAAELILAEGWLRLARGMDVTDLIL
                     QGDNLLPAVAVGLLLAVAIALGSRAFFSRFTPELIRTLFVPILGHTTSREVALMALLP
                     GLGEELLFRGTIQPEIGLVWTSLVFGALHSGFSRELLPYGLWATGVGAILGGLYMVTG
                     NLWGCIAAHSVVNAMGALWLRRMAGTT"
BASE COUNT          430 a          656 c          723 g          411 t
ORIGIN      
        1 gcggctgctg tcggaggtac agcggcgggt ggccgagctg gacacgatca tcggctccat
       61 cgccgacgcc gtggtggtat tcggcccaga catgaaaacc atactgatga acagggccgc
      121 cgagaggatg ctgggataca gcctggagca gtacaggctg gaaccggaaa agcgggtcca
      181 actgctgcgc atggagacgc cggagggtga accgtttcca ctggaggagg ttccatctct
      241 gcgtgccgcg aggggagaaa cggtgagcgg ggtaatcatg gtgttccacc ctccaggccg
      301 aacgatctgg gtctccgtca gcagcggccc gatccgtact cccgacggca ggatatgggg
      361 atctgtcgtc tccatgaccg acatcaccga actgcacagg ctccaggagc agcgggagga
      421 cctcgtgagg atggtctccc acgatctcag gagcccgctg accagcgtcc agagccaggc
      481 ccagatcatc cagaggctgc tgcagagaac cgggggagac gggcagttga ggcagagcgt
      541 cgacgccata tacaccggtg ccagacgaat gaacgccatg atccaggacc tggtggacat
      601 ggcaagggtg gagtcgggtc aactgaccct caaccgcaca aggctggagc tacggtccta
      661 cctggcggag ctgattcaac ggctgaaggg cgtcctggac gtggagaggg ttcggttgga
      721 agtggaggag gggcttcctg aagtcatagc cgacccggac cgtctggaac gggtaatgac
      781 gaacctactg agcaacgccc tcaagtactc acttccggaa acgccggtcc gggtcaccgc
      841 cacctgcatc gatgggaatg tgcagctgtc ggtgaaagat gaaggagtcg ggatagcccc
      901 tgaggacctt cggcgactgt tcgagcgatt ctatcgcgcg agcggcgcac ggaaaaccga
      961 ggggctggga ctggggctgt acatctcccg gatgctggtg gaggcgatgg gcggaaagat
     1021 ctgggtggaa agcgagatag gccagggcag tagcttcaac ttcaccctcc tcctggcata
     1081 atcctttgac agcgtgacac gatccgtggg atacgagacg caaccgagca cagcatcatg
     1141 gccgcgcccg tgcacgactt ccggcatggc cggaatcact gtggtggtca tgccccaggt
     1201 gtgaccgaga cgtagggcgg ggtcttgtgc cccgcctccc ccgacgcgga tgcgaccacc
     1261 ctgtggacca gatgtagcgt cggggattgt ccccgacgtc gtacccgttg ccgccggtca
     1321 ccttaccctg atgcagtgct ggttgccaat ggactacccg tcgcccacaa ggggcgacgc
     1381 tacatccggt caagaggcgt tggtgtagaa tggctggaca atcgttggta ggtgcgcgcc
     1441 atgtccaccg aatccccaca gatccagaga atgatcgcac ggctcacagt tggatccttc
     1501 gccgcggagt tgatcctggc ggagggatgg ctgcggcttg ccaggggcat ggacgtgacc
     1561 gacctgatcc tccagggtga caacctcctg cctgcggtcg cggtgggatt gctgctggcc
     1621 gtggcgatcg cgttgggcag tcgcgccttc ttctcccgat tcacccccga gctgatccgg
     1681 accttgttcg ttcctatcct tggccacacc acctcgagag aggtagcgct gatggcgctg
     1741 ttgcccggtt tgggagagga actgctgttc aggggcacga tccagccgga gatcggcctg
     1801 gtgtggacaa gtctggtctt cggcgcgctg cactcaggtt tctctcgaga actcctcccc
     1861 tacggcctat gggccaccgg ggtgggtgca atcctaggtg gactctacat ggtcaccggt
     1921 aacctctggg gctgcatcgc cgctcactcc gtagtcaacg ccatgggcgc cctgtggctc
     1981 cgacggatgg ccggtaccac ctaagcaact tcccttgttg atatccactt tcccctcaag
     2041 gaaaggccaa ctgcatccag cgaaagttgc ccagccgtgc acgcttcttg cgtctccccg
     2101 ccgccatggc aaagatggcg ttggctcaaa ggacatggat cgtactgctg ccgctccttc
     2161 tggtgatggg gctcagcagt ggctgtgagc tcgacacccg cttcaaggag atcgtgcgcc
//
LOCUS       DATALS010000484         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_73322,
            whole genome shotgun sequence.
ACCESSION   DATALS010000484 DATALS010000000
VERSION     DATALS010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73322"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..748)
                     /locus_tag="VHS06_10805"
     CDS             complement(83..748)
                     /locus_tag="VHS06_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007979458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988645.1"
                     /translation="MGGFSRGWVFGAVTGIAAVSAYRMAIRPWHLRWGATETEASWQL
                     PGDELVPEPKINATHAVTIDAPPEAVWPWIAQLGQGRGGFYSYDWLENLMGLDIHSAD
                     SVIPEYQQIKEGDGIPLAPGGFDFPVVKVEPQRMLLLHGDTRDGSSPVPVKPGEYLNA
                     SWLFLLDAAGRGGTRLVERFRADYDPGQVKGLVYGAFVEPGAFLMERKMLLGIKERAE
                     RLA"
     gene            complement(821..952)
                     /locus_tag="VHS06_10810"
     CDS             complement(821..952)
                     /locus_tag="VHS06_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988646.1"
                     /translation="MVVAIFEMEAEALMWAELPKNAGIPSVLESDTLELVEWRSCGR"
     gene            1067..1357
                     /locus_tag="VHS06_10815"
     CDS             1067..1357
                     /locus_tag="VHS06_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX2988647.1"
                     /translation="MELQEALRILAEHKQELTARGAGSVAIFGSVARNEAGPDSDVDI
                     LVELNQPMGLFGFAELKEYLESILGQRVDLVTPDGLKARIRDRILSEAVRAA"
     gene            1347..1691
                     /locus_tag="VHS06_10820"
     CDS             1347..1691
                     /locus_tag="VHS06_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEX2988648.1"
                     /translation="MPHRQWKVRIDDMLESIERILRYTSGMDFAAFKSDERTIDAVMH
                     NVEIIGEAARHIPAAVEAHNPAIPWTKMRGMRNLLIHQYSDVSIPILWQTVQEDIPPL
                     PPLLRQILEQEP"
     gene            complement(1776..>2781)
                     /locus_tag="VHS06_10825"
     CDS             complement(1776..>2781)
                     /locus_tag="VHS06_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2988649.1"
                     /translation="GWMMGPWAIAGALTLGSTLFIYEGAPDYPGPDRLWEMVARHRIT
                     TLGLSPTVVRGLMRSGEEWIGKHDLSSLRVLGSTGEPWNPESWWWFFDNIGGGRCPII
                     NYSGGTEISGGILGCTVIQPQKPCCFTGPVPGMNAEVLDDRLQPVRGSVGELAISGPW
                     VGMTRGFWRDPERYLESYWSRSPDIWVHGDWATVDEDGFWYILGRSDDTIKVAGKRLG
                     PAEVESAAVAHPAVLEAAAIGVPDEVKGESVVCFALLRPGFAPTDELRAEIKDTVVRH
                     LGKALQPREVRFARELPKTRNGKIMRRVIRAAYLGQEEMGDLSSLENPAAVEEIARAA
                     "
BASE COUNT          532 a         1011 c          746 g          492 t
ORIGIN      
        1 ccccgcctcc cacaccctga ttgcgccctc gtcggtggat tccgatcacg ctgacggcgg
       61 ggcacaagac cccgccctac gattaggcca gccgctcggc ccgctctttg atcccgagca
      121 gcatcttccg ctccatcagg aaggcaccgg gctccacgaa ggcgccgtac accaacccct
      181 ttacctggcc cggatcgtag tccgcccgga agcgctcgac cagtctggtt ccacctctgc
      241 cggcggcatc cagcaggaag agccagctcg cgttcaaata ctccccgggc ttcacgggca
      301 cgggcgagct gccgtcgcgg gtatcgccgt gcaggagcag catccgctga ggctccacct
      361 tcaccaccgg aaagtcgaag ccgcccggag ccagcgggat gccgtcgccc tccttgatct
      421 gctggtactc agggatcacg ctgtcggcac tgtggatgtc gagccccatc aggttctcca
      481 gccagtcgta gctgtagaat ccgcctcgcc cctgccccaa ctgcgcgatc cagggccaca
      541 ctgcctccgg cggagcatcg atggtaacgg catgggtcgc attgatcttc ggctccggca
      601 ccagttcgtc gcccggcagc tgccagctag cctctgtctc cgtcgcgccc caccgaagat
      661 gccagggccg aatcgccatc ctataggcgg atacagcagc gatcccggtc acagcgccga
      721 aaacccatcc cctggagaac ccgcccatct tcccctccct cgacgactcc caccagcagt
      781 cccgctcatg gtacaggctg ggagagtcat cattccaaac tcacctgcca cagcttctcc
      841 attctacaag ctcaagcgtg tccgattcca gcaccgatgg tatacctgca ttcttcggca
      901 actccgccca catcagcgcc tcggcctcca tctcgaagat ggctacaacc acctcgttct
      961 ccagtcctgg cgcagcctcc tctcttgact caccaaagtc gatggctgaa agccgagccc
     1021 caccagtgta tcattgcaat gacgtcgagc cgtcaaggag gtagggatgg agctgcagga
     1081 ggcactgcgc attctggcag aacacaaaca ggaactcacc gcccgcgggg cgggttcagt
     1141 agccatcttt ggttccgtcg cacgcaacga ggccggtcca gattccgacg tggacatact
     1201 ggtagaactt aatcagccga tgggactctt cgggttcgca gagctcaagg agtatctaga
     1261 atcgatcctt ggccagcgtg ttgacctagt cacccccgat ggcctcaaag ccaggatccg
     1321 cgaccgaata ctgtcggagg ccgtccgtgc cgcatagaca atggaaggtt cgcatagacg
     1381 acatgctcga gtctatcgaa cgcatcctga ggtatacatc aggtatggac ttcgcggcat
     1441 tcaagagtga cgagaggacc atagatgcgg tgatgcacaa cgtcgagatc atcggtgaag
     1501 cggcccgcca catcccggca gcggtggagg cacacaatcc agcaatcccc tggacaaaga
     1561 tgcgtggcat gcgcaacctg ctgatccacc agtactccga cgtcagcatc cccatcctct
     1621 ggcagacggt gcaggaggac attcctcctc tcccacctct gcttcgccaa atcctggagc
     1681 aggagccgta acgctcacct gggggaaagc caccgttcag ttggggttct tacctccccc
     1741 tctatggcac catcgtccgg catagccccc ttcccttacg ctgccctcgc tatctcctct
     1801 acggccgcgg ggttctccag cgacgagaga tcccccatct cctcctgtcc caggtaggca
     1861 gccctgatca cccttcgcat gatctttccg ttccgcgtct tcggaagctc ccgagcgaaa
     1921 cgcacctccc gcggctgcag cgccttgccc agatgccgaa ccaccgtgtc cttgatctcc
     1981 gccctcaact cgtcggtcgg cgcaaagccc ggcctcagca gcgcgaagca caccaccgac
     2041 tcgcccttca cctcgtccgg aacaccaatg gccgccgcct ccaggaccgc cgggtgtgcg
     2101 actgcggcgg actccacctc cgcgggccca agccgcttcc ccgcgacctt gatcgtgtcg
     2161 tcggagcgcc ccaggatgta ccaaaaaccg tcctcatcca ccgttgccca gtccccgtgc
     2221 acccagatgt cggggctccg agaccagtag gactccaggt agcgctccgg gtcgcgccag
     2281 aagccacggg tcatgcccac ccacggccca ctgatcgcca actctccgac gctcccgcgc
     2341 accggctgaa gccggtcgtc caacacctca gcgttcattc caggcaccgg cccggtaaaa
     2401 cagcagggct tctgtggctg gatgacggtg caaccgagaa tcccgcccga gatctccgtg
     2461 ccgccggagt agttaatgat ggggcaacgg ccccctccga tattgtcgaa gaaccaccac
     2521 caggactccg ggttccacgg ctcgccggtc gatcccagca cgcggaggct gctcaggtcg
     2581 tgtttcccta tccactcctc cccgctgcgc atcagtccac ggaccaccgt gggcgaaagg
     2641 cccagggtgg tgatgcgatg tcgcgccacc atctcccaga gccggtcggg cccgggatag
     2701 tccggcgcgc cttcgtatat gaacagggtg ctgcccaggg tcaatgcgcc ggcgatggcc
     2761 caggggccca tcatccaccc c
//
LOCUS       DATALS010000485         4826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_73550,
            whole genome shotgun sequence.
ACCESSION   DATALS010000485 DATALS010000000
VERSION     DATALS010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73550"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(464..601)
                     /locus_tag="VHS06_10830"
                     /pseudo
     CDS             complement(464..601)
                     /locus_tag="VHS06_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015124853.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
     gene            858..4100
                     /locus_tag="VHS06_10835"
     CDS             858..4100
                     /locus_tag="VHS06_10835"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEX2988650.1"
                     /translation="MIGAVVCTALPASEGPCARDLKCEYLVNPQGIDVTNPRLSWVLE
                     SAERGTVQTAYHILVSGSEDALAHDRGDLWDSGKVGTDQSIHVVYAGRPLPSQRRCFW
                     KVRVWDRHGNVSKWSKPAQWSMGLLNRLDWKGKWIGLDEGENSDYFAQAQWIWSPDES
                     ADTAAATRLFRRTVDIPDGREISGARLTIATRGQFAIWVNGAQVVAAARTVFAPVSEI
                     DLEKYLSPGVNTVAVAASATGKKEDPSGLICALSAEFESGEPLVIVSDASWRVSSRET
                     NGWERPGFDDSRWASAKELGGNGVPPFANVAGDEYRRLPARMLRRELQVDRKIKRATV
                     YMCGLGLSELYINGRRIGDHVLSPGLTDYNKRSLYVTYDVAKDLRQGTNAVGVVLGNG
                     RYFAPRRSSPTTTVSYGYPKLLFQIQTEYEDGSVSTVVSDESWKITDSGPIRANNEYD
                     GEVYDARKEMNGWSEAGFEDSSWRTASIVDAPKGVLSSEMAEPIRVMEKLRPIAVTSP
                     SPGVHIFDMGQNMVGWCRLKVKGPRGTRVSMKHAERLNPDGTLYLANIRSAKATDTYI
                     LRGGGGEVYEPRFTYHGFRYVEITGLPYEPDLLTLEGMVIHDALQRTGEFSCSNQLLN
                     RIYQNSCWGIRGNYRSIPTDCPQRDERQGWFGDRAQVSRGEMYIFDVAALYTKWIRDM
                     EDSQLPDGSIPDIAPAFWPFYTNSITFPTAALAIPDHLYQLYGDLRILETHYQSAKGW
                     VDKASERLTGDTMPRDTYGDWCVPPESLEMILSSDPNRVTDPELVSTAYFHYDLRLIA
                     RWAALLGRKEDAARYGDLADRVKTAFNRKFFNETTGIYGNGTQTSSVLALAYNLVPEE
                     HKERVVANLVENVLVKSDCHIGTGMVGCQWLMRALSDNGRPDVAYKLASQTTYPSWGY
                     MVEQGATTIWELWNGDRGDPLMNSGNHVMQIGDLCTWLHEYVAGIAPDSRCPGFKRVV
                     MRPCITAGLTSARAYHKSMYGRIASDWKIADGTFSWRIEIPANTTGAIYVPAKDAELV
                     AEGGRPAKGATGVRFLRMEDGRAIFEVGSGRYLFTSQVDLPRRGSQGNVH"
     gene            complement(4157..>4826)
                     /locus_tag="VHS06_10840"
     CDS             complement(4157..>4826)
                     /locus_tag="VHS06_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2988651.1"
                     /translation="REGVPVMYLPVPLQAELLTRAGVKDNQTVIIYSDGESVPGATMM
                     AYVLEKIGHPDIKIIDGGWTAYKATQKTSQQYPRYKPGRLSVHQSKTYVTTDEVVKAA
                     GKPGYKLIDARDRDDYLGQTKLFMRNGHIPGAISIPWPSLMEKENQHKFKAAAEQQAI
                     YDANGVKKSDTIILYCGTSRDASVQYAVLKHMLGYPKVLLYEGSWTEYVSHPELPIKV
                     GPEP"
BASE COUNT         1009 a         1365 c         1491 g          961 t
ORIGIN      
        1 tcgagtatgt cgtggtcatc gaagacaagc agtggaaggt ggacctgaag aagacaagca
       61 aagtgtcaac gccccgcgtg gcgctcccga tcgggcgctg aggtccgttt cgaggtggtc
      121 tacccagtcc aggattggcg ggaactccac aatcgagagc actctgcagc tcatcgagag
      181 gcactctggg tttgtttgag gctcttactt actttgcgta caacagcggt tggttcaccg
      241 atagcccagt tcgacctctc cccctgcacc ccctccccga cgcggggatg gggagactct
      301 cgaagagagt acgaaagtct tttgttctaa cggggctacg atccctcccc gtgtcgggga
      361 gggtggccga aggccgggag aggtctaaca cagctaacga caaacgaacg gatgttatca
      421 gcagactgag ttagggccct gtttgatgcg gtcccactcc cccctacggc tccggcccgg
      481 tccttattgg cagctccgga tgcgaaacgt attccgtcca cgaaccctca tagagaagca
      541 ccttcggata accgagcacg tgcttgagga tggcgtattg aacactcgca tcccggctgg
      601 ttggcatgct tcggcgcgga tactgctctt gcgccgaagc atacagaaca tctgttccat
      661 caactgcagg agcgcagctt cgcaggacgt ccgtgtccat ttggaaagga ttcaccgttg
      721 tctccgccta acaggtccgc atgtggtggc ttacaccgcg ccaatcggga gcagacatgc
      781 agacacggat gggatctggc aatgtcctca caaagcaagt ggtgttcctt ggccaaatcg
      841 ttgcggaggt gtttctgatg atcggcgcag ttgtgtgtac agcgctacct gcaagcgaag
      901 gcccctgtgc gcgagatctg aagtgcgagt acctggtcaa cccgcaaggt attgacgtga
      961 cgaatcctcg cctgagctgg gtgctggaat cagcagaacg gggaacggtt cagacagcct
     1021 atcacatact cgtgtcaggc agcgaggatg ccctcgccca tgacagaggt gatctctggg
     1081 acagcggcaa ggtcgggacg gatcagtcta tccacgtcgt ctatgcaggc agacccttgc
     1141 catctcagcg caggtgcttc tggaaggtcc gcgtgtggga caggcacgga aacgtctcga
     1201 agtggagcaa gcctgcccag tggtctatgg gcctgctgaa tagactggac tggaagggca
     1261 agtggatcgg cctcgatgag ggtgagaact cggactactt cgcccaggca cagtggatat
     1321 ggtcccctga tgaatctgca gatacagcgg ccgcaacccg gcttttccgt cggactgtag
     1381 atattcccga tggacgcgag atcagtggcg cgcgcctgac gatcgccacc cgtggccagt
     1441 ttgcgatctg ggtcaacggt gcacaggtcg tggcagctgc tcggacggtc tttgcgccgg
     1501 tttccgagat agatctggag aagtacctga gccccggcgt caacacggtt gcggtggctg
     1561 cttcagcaac cggcaagaag gaagatccat cgggactgat atgcgcgcta tccgccgagt
     1621 tcgagtccgg tgagccgctg gtgattgtct cagacgcgag ctggagagtc tcctcgcgcg
     1681 agaccaatgg ctgggagaga ccagggtttg atgactcacg ctgggcgagt gccaaagagc
     1741 ttggtggaaa tggggttccg ccgtttgcca acgttgcagg cgacgagtac cggaggctgc
     1801 ccgcgcgcat gctccggcgc gaactccagg tggatcggaa gataaagcgg gctaccgtct
     1861 acatgtgcgg cctcggactc tctgagctat acatcaacgg ccggagaatc ggcgatcacg
     1921 ttctttcgcc gggcctcacc gactacaaca agcgctcact ctacgtcact tacgacgtcg
     1981 caaaggacct caggcaaggc accaacgcgg ttggggtagt cctcggcaac ggacggtatt
     2041 tcgctccccg ccgcagcagt cccaccacca cggtgagcta cggttatccc aagcttctct
     2101 tccagattca gaccgagtat gaagacggct ccgtctcgac tgtcgtgagt gacgagtcct
     2161 ggaagatcac cgacagcggg ccgattcgcg ccaacaacga atacgacggt gaagtctacg
     2221 atgcaaggaa ggagatgaac ggctggagcg aggcgggatt cgaggattcg agctggcgga
     2281 ctgcttccat agttgatgcc ccgaagggcg tgctgagctc ggagatggcc gagccgatcc
     2341 gagtgatgga gaagctgcgt ccaatagcag tcaccagccc ctcacccgga gtccacatct
     2401 tcgacatggg ccagaacatg gtcggatggt gccgtctgaa ggtcaaaggt ccacggggga
     2461 cgagggtttc gatgaagcac gctgagagac tgaaccccga cggcacgctc tacctggcca
     2521 atatccgctc ggctaaggcc accgacacct atatcctgag aggcggcgga ggcgaggtct
     2581 acgaaccgag gttcacctat catggcttca ggtatgtcga gatcacaggc ctgccgtacg
     2641 agcctgatct cctaacgctt gagggaatgg tcatacacga tgccttgcaa agaacaggtg
     2701 agttctcgtg ttcgaaccaa ctcctgaacc ggatatacca gaacagctgc tggggaatac
     2761 gaggcaacta caggagtatt cccaccgatt gcccacagag agacgagcgc cagggatggt
     2821 tcggcgacag ggcgcaggta tccaggggcg agatgtacat cttcgatgtg gccgcgcttt
     2881 ataccaagtg gatacgcgac atggaggatt cccagctacc tgacgggagc attcccgata
     2941 tcgctcccgc tttctggcca ttctatacca acagcatcac gttcccgacc gctgctctgg
     3001 ccatccctga ccatctatac cagctgtacg gcgacttgcg gatactggag actcactacc
     3061 agagcgcaaa gggatgggtc gacaaggcga gtgagcgcct gacaggcgat acaatgccca
     3121 gggataccta tggagactgg tgtgtgccgc ccgaatcgct ggagatgatc ctgtcctctg
     3181 atccgaaccg tgtgaccgat cccgaactgg tctccacggc atacttccac tacgatctgc
     3241 gcttgatcgc ccgctgggca gccctgctgg gcaggaaaga agacgctgca cgctatggcg
     3301 accttgccga ccgcgtgaag actgcattca acaggaagtt cttcaatgaa acgaccggta
     3361 tctacggcaa tggcacccag acatcgagcg ttcttgcatt ggcatacaat ctggttccgg
     3421 aggagcacaa agagcgtgtc gtcgccaatc tggtcgaaaa cgtgctggtc aagagcgact
     3481 gtcacatcgg cacgggtatg gtaggctgcc agtggctgat gcgggctctg tcagacaatg
     3541 gacgcccgga tgtcgcctac aagcttgcct cacagacgac ctacccgagc tgggggtata
     3601 tggtggagca gggcgcaacg accatctggg aactgtggaa cggcgatcgc ggtgacccct
     3661 tgatgaactc aggcaaccat gtcatgcaga tcggtgacct ttgtacctgg ctccatgagt
     3721 atgtggcggg cattgcgccg gacagccgtt gtccggggtt caagcgcgta gtaatgcgtc
     3781 cgtgtatcac ggcgggcctg acgtccgcca gagcgtatca caagtccatg tacggcagga
     3841 ttgcgagcga ctggaagata gcagatggca ctttcagctg gcggatagag atccctgcga
     3901 acacaaccgg cgccatctac gtgcccgcga aagacgctga gctcgtcgca gagggcggca
     3961 gacccgcgaa gggggctacc ggtgtacggt tcctgcggat ggaggacggg cgggcgattt
     4021 tcgaggtagg atctgggcgc tacttattca cgtcccaggt cgaccttcct cgaaggggaa
     4081 gtcaagggaa tgtgcactga gcgcggcagg cagggcccga gtcgttcgag atggatatgc
     4141 ggaacccctc tcgcccctac ggctccggcc cgaccttgat cggcaactcc ggatgcgaaa
     4201 cgtactccgt ccaggaaccc tcgtagagaa gcaccttcgg atagccgagc atgtgcttga
     4261 ggacggcata ctgaacgctc gcgtcccggc tggtgccgca gtagaggatg atggtatcgc
     4321 tcttcttgac cccgttcgcg tcgtagatag cctgctgctc ggcggcggcc ttgaacttgt
     4381 gctggttttc cttctccatg agcgagggcc aggggatgct gatggcgccg gggatgtggc
     4441 cgttgcgcat gaacagcttc gtctggccca ggtagtcgtc cctgtcgcgc gcgtcgatca
     4501 gcttgtagcc gggcttgcct gccgccttca ctacctcgtc ggtagtcaca taggtctttg
     4561 attggtggac cgagagcctg ccgggcttgt atctggggta ctgctgggaa gtcttctgcg
     4621 tggccttgta ggcggtccag ccgccatcga ttatcttgat atcgggatgg cctatctttt
     4681 ccaggacata ggccatcatg gtcgctccgg ggacgctctc gccatcggag tagatgatca
     4741 cggtctggtt gtccttgaca ccggcccgcg tgagaagctc ggcctgcagc ggcaccggca
     4801 gatacatcac cgggacgccc tccctc
//
LOCUS       DATALS010000486         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_121676, whole genome shotgun sequence.
ACCESSION   DATALS010000486 DATALS010000000
VERSION     DATALS010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121676"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /locus_tag="VHS06_10845"
     CDS             <1..1830
                     /locus_tag="VHS06_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2988652.1"
                     /translation="VSPTPRATHASPLRDQSGAVPDPALRTQNPELGTDEELRFQLYD
                     AIAHHPEIAGLSAQACERLGRLRSLLDGLARSVGVLPLAELVERTVEGAGYRRELTAR
                     GDFDSRLALLNIGKLVELARQFQGADGTLSGFVEYVQYALESGGEEAEVRPVDEGSDA
                     VKVMSVHQAKGLEFPVVFVPGLAEKIFPDPRIDDPDRWDQFPDQLRGDRSRRDVRAFR
                     EIPADKATTQARKEAAREREMEEERRLFYVALTRAQQTLFLSRAWWYFTNSKARNGSP
                     FWDEVVDAKEDGVPISTPLGEEEVPPANPRLQGNGALTGQKAEGMKSRAGLLLVEDRG
                     ASWISQVAETDHAQWQKRRAEVDRRLALLGEESLAGTALPSVETSCTGLLQYDECPRL
                     FRYLQVDRLPERPSPSAALGIEVHRLIEEFTQGDDQDGAGDREWDMEAEEVERADGQQ
                     IEATPMDLLGIYRRSRYGRRLATRMEEDFTLPLGPNLLRGRVDRLDLLPNGSWEMVDF
                     KSGRFPGRVDRRYRLQLQLYSLAAWQLWGIEPDRLSCHLFFLASDHDEALHFSGQELR
                     ETEAWALERLDRIRQGIFPRTEHPEPCLRCGYGQVCTSSLDRH"
     gene            complement(2023..2220)
                     /locus_tag="VHS06_10850"
     CDS             complement(2023..2220)
                     /locus_tag="VHS06_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX2988653.1"
                     /translation="MDRFQAVGQWNQLKGKIKERWGELTDDDIMQTQGNIDQLIGKIQ
                     EKYGGTTEEIRQEIEEMRSGM"
BASE COUNT          424 a          740 c          789 g          383 t
ORIGIN      
        1 gtatcgccca cgccacgggc gacgcatgcg tcgcccctac gagaccagtc cggtgccgtg
       61 ccagacccag cactcagaac ccagaaccca gaactcggca ccgatgagga gctgcgcttc
      121 cagctgtacg acgccatcgc ccaccatccc gagatcgccg ggctgtcggc gcaggcatgc
      181 gagcgtttgg ggaggctccg gtcgctgctg gacggactcg cccgctccgt cggagtgctt
      241 cccctggccg aactggtgga gcggaccgtg gagggggccg gctaccgccg agagctgacg
      301 gcgaggggcg acttcgactc caggctggcg ctcctgaaca tcggaaagct ggtggagttg
      361 gcccgccagt tccagggggc cgacgggacg ctgagcggct tcgtggagta cgtgcagtac
      421 gcactggaga gcgggggaga ggaggccgag gtccggcccg tggacgaggg gagcgacgcg
      481 gtcaaggtga tgagcgtcca ccaggccaag gggctggagt ttccggtggt gttcgtcccg
      541 gggctcgcgg agaagatctt ccccgacccg aggatcgacg acccagacag gtgggaccag
      601 ttcccggacc aacttcgcgg ggatcggtcg aggcgagacg tgcgggcttt ccgggagatc
      661 ccggcggaca aggcgacaac acaggcccgc aaggaggcag ccagggagcg ggagatggag
      721 gaggaacggc ggctgttcta cgtcgccctc acccgggccc agcagacgct gttcctctcc
      781 agggcgtggt ggtacttcac caacagcaag gctcgaaacg ggtccccctt ctgggacgaa
      841 gtggtcgacg ccaaggagga cggcgtgccc atctccactc cactgggcga ggaggaggtt
      901 cccccggcaa acccgcgcct ccagggaaac ggagcgctga cagggcaaaa ggccgagggc
      961 atgaagagcc gtgcagggtt gctgctggtg gaagaccggg gggcatcgtg gatcagtcag
     1021 gtggcggaga cagaccacgc acaatggcag aagcgcaggg cggaggtgga tcgccgtctg
     1081 gcgctgctag gcgaagaatc cctcgccggc acggcgctcc cgtctgtgga gacctcctgc
     1141 accgggctgc tgcagtacga cgagtgccct cgcctcttcc gctacctcca ggtcgaccgg
     1201 cttcccgagc ggccctcccc cagtgccgcc ctgggcatag aggtgcaccg actgatcgag
     1261 gagttcaccc agggcgacga tcaggacggc gcaggtgacc gggagtggga catggaagcc
     1321 gaggaggttg agagagccga cggccagcag atcgaggcaa cgccgatgga tctgctgggc
     1381 atctaccgtc gaagtcgata cggccggagg ctggccacaa ggatggagga ggatttcacc
     1441 ctgccgctgg gtcccaacct gctgcgcggc cgggtggacc ggttggacct tctgcccaac
     1501 gggagctggg agatggtgga cttcaagtcg ggccgcttcc ccggtcgggt cgaccggcgc
     1561 taccggctgc agctgcagct gtactcgctg gccgcctggc agctgtgggg gatcgagccc
     1621 gaccggctca gctgccacct cttcttcctg gccagcgacc acgacgaggc gctgcacttc
     1681 tcagggcagg agctgaggga gaccgaggcg tgggcgttgg agaggctgga tcgcatcagg
     1741 caggggatct tcccccgcac ggagcacccc gagccgtgcc tccgctgtgg gtatgggcag
     1801 gtgtgcacat cctccctgga cagacactag cgtatgacat actgaagggg atacccactg
     1861 tcacccttcg atgcgctcag catctcctcg tgaagggaga gatccttcgc ctgcggctca
     1921 ggatgacact caccaagatc cgttcgttac ggtagtaggc atgtgaaacg gaggggaggg
     1981 ttgagacaac cctcccctcc gtttcgagag cagcacgccg agctacatcc cgctgcgcat
     2041 ctcctcgatc tcctggcgga tctcctcagt ggtgccgccg tacttctcct ggatcttgcc
     2101 gatcagctgg tcgatgttgc cctgagtctg catgatatcg tcgtcggtga gttcgcccca
     2161 ccgttctttg atcttcccct tcagctgatt ccactgccca accgcctgga atcggtccat
     2221 cttctcgtaa tcctcctata tgaccttcct cgaatatccc gacggtcgtc ggtcacaagc
     2281 aagaccggcg ccaggatcgg ccacccgcac caaccactct tctgctacaa tagctc
//
LOCUS       DATALS010000487         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_354978, whole genome shotgun sequence.
ACCESSION   DATALS010000487 DATALS010000000
VERSION     DATALS010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354978"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1459
                     /locus_tag="VHS06_10855"
     CDS             143..1459
                     /locus_tag="VHS06_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2988654.1"
                     /translation="MSSAKSGWRHRVPFFYGWVVVASVFLAMSVCYGVYYTFSVFFVA
                     LLEQFGWSRASTAGVFSLFVMATAVGSVASGALIDRFGPSRVIPVGGMLLAAGLFGTS
                     RLTELWEFYLYFGVLCGLGLAFAGWVPGVIVVTSWFSTRQGLAMGIASAGNGLGTVVF
                     VPLSQYVISTAGWRSAYLLLAGLALFCIAPQAALLLVHRPEDLGMKPDGVVGGSTDVS
                     SRRPPKREIMVVDREWASRRWSVADAVGTGRFWLLTATLALAVLTHQMLFVHQAAYLV
                     DGGYDRMLAASVVGLIGLASIPSKIMWGIVCDRLGRERAFTLGTVALLVGIALLVLAR
                     VVPSPAILLLYVAVFAMGYAATPPILSTAAADVFGGRSFGAIYGLVCIGQGVGSAFGA
                     WVAGYIFDVSGSYAAAFIVGALSAIAGSGCLWLAAPRMVRRVVRKA"
     gene            1676..1750
                     /locus_tag="VHS06_10860"
     tRNA            1676..1750
                     /locus_tag="VHS06_10860"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1708..1710,aa:Ala,seq:ggc)
     gene            1915..>2795
                     /locus_tag="VHS06_10865"
     CDS             1915..>2795
                     /locus_tag="VHS06_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2988655.1"
                     /translation="MANHVEKYNPSEIEPKWRAKWEEMGIYKVKTDPSKPKHYALVMF
                     PYTSGDLHVGHWYNFGPADTHARYKRMQGYNVFEPIGFDAFGLPAEEAAIKHGINPAD
                     WTERNIDNMTKQLKTIGAMYDWSHMVAACRPEYYKWNQWFFLQFYKRGLAYRAKAPAN
                     WCPHCETVLANEQVEDGKCWRCDTVVTRRDLEQWFFKITAYADELLDFSEIDWPERVV
                     TMQRNWIGRSEGVEFTMKVDGTSDSFTVFTTRPDTIYGITYAVLAPEHPLVEKLTTPE
                     RRAEVTAYVEQARRQSE"
BASE COUNT          441 a          890 c          899 g          565 t
ORIGIN      
        1 ccggctcctg gctcgggggc tacgtattcg acaccacagg ctcctacatg cttgccttcg
       61 cgctgggtgc tatctgctcc ataaccagcg ccctgtgctt ctggctcgcc gcgcctcgca
      121 aggttcgcca cgtggtgttc aagtgagctc tgcgaaatcg ggctggcgcc accgtgtgcc
      181 cttcttctat gggtgggtcg tcgtcgcgtc ggtcttcctg gccatgtccg tctgctacgg
      241 cgtctactac accttctctg tcttcttcgt tgcgctgctg gagcagtttg gctggagcag
      301 agcctccacg gcgggcgtgt tttccctctt cgtcatggcc accgccgtcg gcagcgtggc
      361 gagcggcgcc ttgatcgacc gtttcgggcc ttcccgtgtg atccccgttg ggggcatgct
      421 gctggccgcc ggactgttcg gcaccagccg gctgaccgag ttgtgggagt tctacctcta
      481 ctttggtgtc ctctgcgggc tggggctggc ctttgccgga tgggtccccg gggttatcgt
      541 ggtgacctcc tggttctcta ccaggcaagg cctggccatg ggtatcgcat ccgcgggcaa
      601 cggactgggg accgtagtct tcgttccttt gagtcagtac gtgatctcca cggcgggctg
      661 gcgctccgca taccttctgc tggccggcct ggccctcttc tgcatcgcgc cccaggccgc
      721 actgctgctt gtgcaccgcc ccgaggatct ggggatgaag cccgacggcg ttgtcggagg
      781 atcgaccgac gtctcatccc gtcgaccccc caagagggag atcatggttg tggatcggga
      841 gtgggcgtcc cgccggtggt ctgtggccga cgcggttggg accggccgct tctggctgct
      901 caccgcgacc ctcgccctgg ccgtcctgac ccaccagatg ctgttcgttc atcaggccgc
      961 ctatctggtg gatggcggtt acgatcggat gctggccgcc tccgtggtgg ggttgatcgg
     1021 gctggccagc ataccctcga agatcatgtg ggggattgtg tgcgatcgac tgggccgtga
     1081 gcgcgccttc accctgggca ccgtcgccct cctggtaggg atagctctgc tggtgctggc
     1141 cagggtcgtc ccctcgccgg ccatcctgct gctgtacgtc gccgtcttcg ccatgggcta
     1201 cgcggctacc cctcccatac tctccacggc ggcggcggat gtcttcggcg gcaggagctt
     1261 cggggcgatt tacggtttgg tgtgcatcgg ccagggtgta gggagcgcct tcggggcctg
     1321 ggttgccggc tacatcttcg acgtcagcgg ctcctacgcc gcggccttca tcgtgggggc
     1381 cctgtcggcg atagccggtt ccggctgtct ctggttggcc gcgcctcgaa tggtgcgccg
     1441 agtggtgcgc aaggcttagc tcttgcggcc cagccttcct ttctggtatc atgtgcgggc
     1501 gttgagcggg agtagtaagt cgcgccaagc acagagagtc gggagggtgg aaaccgactg
     1561 ggcaggcatc gagccgacgg ctgaactcgc ccgggagcag cagcggtgaa gctcagtagt
     1621 cgctgccggc cggcgaccgt tatgacgccg aagagcggac gagcgttttc cacatggggc
     1681 agtggcgcag cgggagcgcg cttccttggc atggaagagg tcaggggttc aattcccctc
     1741 tgctccacca atacaaaccc tcgtcaagca gggtggtacc gcggaacggc ctttcgcccc
     1801 tgaacaggag cgaagggctt tcgcattgta tggggcattt tctgtgagga ttaccctcac
     1861 cctgaccttc tcccagaggg agagggagac ttgcgaggtt agggagaacg gcatatggcc
     1921 aatcacgtag agaagtacaa tcccagcgag atcgagccca agtggcgcgc caagtgggaa
     1981 gagatgggga tctacaaggt caagacggat ccctccaagc ccaagcacta cgccctcgtc
     2041 atgttcccct acacctcggg cgacctccac gttggccact ggtacaactt cggtccggcc
     2101 gatacccatg cccgctacaa gcggatgcag ggctacaacg tcttcgagcc gatcggattc
     2161 gacgcattcg gcctaccggc tgaggaagcc gccatcaagc acggcatcaa ccccgccgac
     2221 tggactgagc gcaacatcga caacatgacc aagcagctca agaccatcgg cgccatgtac
     2281 gactggtccc acatggtcgc ggcctgccgg cccgagtact acaagtggaa ccagtggttc
     2341 ttcctccagt tttacaagcg aggtctggcc tatcgtgcca aggctcccgc caactggtgc
     2401 ccccattgcg agaccgtgct ggccaacgag caggtggagg acgggaagtg ctggcgctgc
     2461 gacaccgtgg tgacccggcg tgacctggag cagtggttct tcaagatcac cgcctacgcc
     2521 gacgagctgc tggacttcag cgagatcgat tggccagagc gggtcgtgac gatgcagcgc
     2581 aactggatcg gtcgcagcga gggtgtcgag ttcaccatga aggtggacgg cacctccgac
     2641 tcctttaccg tcttcaccac ccgcccggac accatctacg gcatcaccta cgccgtcctc
     2701 gccccggagc acccgctcgt ggaaaagctg accaccccgg agcgtcgagc cgaggtcacg
     2761 gcctacgtcg agcaggcccg ccggcagtcg gagat
//
LOCUS       DATALS010000488         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_445171, whole genome shotgun sequence.
ACCESSION   DATALS010000488 DATALS010000000
VERSION     DATALS010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445171"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..1071)
                     /locus_tag="VHS06_10870"
     CDS             complement(421..1071)
                     /locus_tag="VHS06_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988656.1"
                     /translation="MAAKRLLLLLALTLLVLSATTVGVSAQTSTPGDETQVKVTISGP
                     ENGDSAGLSGTGWLVDLRLDFNATLDQTGFNGFALTGPGGHTDIAPFAAGFAPGKAGK
                     MPGLVVLISTSSVGAGQGQNLAGLFNLTGVTDLDSSVPTSQLRDTWIITQPAFGVDTP
                     STVLAAVVADLDGNGMFDDAPNVVVDANGDGVIDEADLTAIGLASNIAKADFFINP"
     gene            complement(1297..2400)
                     /locus_tag="VHS06_10875"
     CDS             complement(1297..2400)
                     /locus_tag="VHS06_10875"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX2988657.1"
                     /translation="MRRLLITMAMAAIVSSLAIAGCSQGAAPAASQASPTAAKAAEPT
                     KATAASPTKASEPTKAATAPAKTTSFPEKGKSVSLIVGYAAGGGADIGARVLASPLEK
                     ELGTPVQVINKAGAGTQVSTTFVAQSKPDGYTVGYSNWPNMITIYMDPERKATYGRKD
                     LQPIAMHLVDPMAIAVKGDSPYKDLKDLIQAAKDKPKEIKIGTTGKLAIEHLVFLKLQ
                     EEVGVKFTFVHFDGTAPGITALLGGHIDAIGGGFSSVLPTAKAGQTRVLASMDKGGDA
                     FLPGTKSLEDLGYKGYFGVSRGWFVPAGTPKDVVNVLTNGIKKSMETEEHKTKIKEIG
                     QMPMFMGPDEMATYWDNQDTWVKPLMALAAKDE"
BASE COUNT          470 a          770 c          857 g          535 t
ORIGIN      
        1 agtgccgcgg agatggcgcc gctgatcagc aggtcgtaca ccatcgtggg gacggtggag
       61 gcggctgtcc agacgtctgt ggcaatcttc gccccgaaga gggcggcgat cacctgctcc
      121 cgcaccagcc ccagcaggcg ggagatgatg ttgcccagca tgatgatgcc ggcggccttg
      181 gcgatgtgag agcggttgtc caggggagcc tccgagagat cgatcgggcg acatgatatc
      241 gagtcggggg gcgtgcgtcg aatctgccgt gcggtcgtcg ctttcgaagc cggtgctcaa
      301 ggagcggagg gcagagaagg gtaactatgc catggcattg gaaacggcgc gatgcggtga
      361 gatcggggat gtggcgggcg actccatgca cgagatcgcc cgccacaaat ctcaagggcg
      421 ctaagggttg atgaagaaat cggccttggc gatattcgag gccagcccta tcgcggtcaa
      481 gtcggcctcg tcgatgacgc catccccatt ggcgtcgacg accacgttgg gagcatcgtc
      541 gaacatcccg ttgccatcca gatccgcaac tacagcagcc agcacagtgc tcggcgtgtc
      601 taccccaaag gccggctggg tgatgatcca ggtatcccgc aattgcgaag tgggcacgct
      661 gctatccagg tcggtcactc ccgtgaggtt gaacagcccc gcgaggttct gcccctgccc
      721 tgcacccacg gaacttgtgg atatcagcac gacgagaccg ggcatcttgc cggccttacc
      781 cggcgcgaac ccggcagcga aaggcgctat gtcggtatgc ccgccggggc cggtcaacgc
      841 gaacccgtta aaaccggtct ggtccaacgt ggcattgaag tctaaccgga ggtcgacgag
      901 ccacccggtt ccactcaggc ccgcactgtc gccgttctcc ggaccagaga tggtgacttt
      961 gacctgtgtc tcgtcgcctg gggtggaggt ttgagcggag actccaacag tagttgccga
     1021 cagtacgagg agtgtgagcg ccaagaggag tagcagtcgc tttgcagcca tcagttccct
     1081 tctatccttt cgatgcaagt gttggccatc cggcgaggtt cgatcgacga acatctgggg
     1141 gacctgcaac atggatgcca gctctttcgc agaggtcgat cggttcttga cggtcaaggg
     1201 ctctgaggcg tgtgtggttt ctgaaagccc ggcggtagat tgggggaatg ggatgaggga
     1261 ccggggcggg cttgtggccc gccccggtag gggttgctat tcgtcctttg cggccagagc
     1321 catgagcggc ttgacccagg tgtcctggtt gtcccagtag gtcgccatct cgtcggggcc
     1381 catgaacatc ggcatctgcc cgatttcctt gatcttggtc ttgtgctcct cagtctccat
     1441 cgacttcttg atgccgttgg tgagcacgtt cacgacgtcc ttcggtgtgc cggccgggac
     1501 aaaccagccg cgagaaacgc cgaagtaccc cttgtagccg aggtcctcca ggctcttggt
     1561 gccgggaagg aaggcgtcac cacccttatc catcgaggcc aggacgcggg tctggccggc
     1621 cttcgcggtg gggagcacag agctgaagcc tccgccgatc gcgtcgatgt gtccgcccag
     1681 cagcgccgtg atgccggggg ccgtgccgtc gaagtgaacg aaggtgaact tgacgcccac
     1741 ctcctcctgc agcttcagga agacgaggtg ctcgatggcc agcttgcccg tggtgccgat
     1801 ctttatctcc ttgggcttgt ccttcgcggc ctggatcaga tccttgagat ccttgtaggg
     1861 gctatcgccc ttcacagcga tggccatggg atcgacgagg tgcatcgcga tgggctgaag
     1921 atcctttcgc ccgtaggtgg ccttgcgctc gggatccatg tagatcgtga tcatattggg
     1981 ccagttggag tagccgacgg tgtagccgtc gggcttggac tgtgccacaa aggttgtcga
     2041 gacctgtgta ccggcacctg ccttgttgat cacctgtacg ggagtgccca gctccttctc
     2101 cagcgggctt gccagaacgc gtgcgccgat gtcggcccct ccgccggccg cgtagccgac
     2161 gatcaatgag acagacttgc ccttctccgg gaaactcgtc gttttggcag gcgcggtggc
     2221 tgcctttgtg ggctccgaag ccttggtagg gcttgctgcc gtggcttttg tgggctcggc
     2281 ggccttggct gctgtagggg atgcctggga tgctgcgggc gcagctcctt gcgagcaacc
     2341 ggcaatggcc agggagctca cgatggctgc catcgccatg gtgatgagaa gacgcctcaa
     2401 ttcctcctcc ttctcgttgc ctgtgctatg gatgcacagg ttgcgctgtg gattgccgca
     2461 gaagctgcag cctgtcgcgg aaaacaaaaa tcccgtcccc caagagggac gggattaccc
     2521 gcggtaccac ccaaattgat gctgcctggt gcaccaggac ggcacacgac aacaaaaaaa
     2581 cctcgtcccg tagggacgag gccttagcat gctcgtggta ccaccctagt tg
//
LOCUS       DATALS010000489         3208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_355215, whole genome shotgun sequence.
ACCESSION   DATALS010000489 DATALS010000000
VERSION     DATALS010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355215"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..839
                     /locus_tag="VHS06_10880"
     CDS             204..839
                     /locus_tag="VHS06_10880"
                     /inference="COORDINATES: protein motif:HMM:NF025064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988658.1"
                     /translation="MAAGSGFAKLFEDLGACAIVPGGQTMNPSIEELVHAAASCQSEK
                     VILLPNNPNVILTAQQAQSLAKANIQVIPTRTICEGVAAMVAFNADADLDVNVGGMKE
                     AADAIHTIEVTRAVRSTRVNGLEVAEGQAIGLVDGNLVNAGDSVADVALMGLDRVGAG
                     DHEVVTLYRGDGVSEVEGRDLAERITERWPDLQVDTVDGGQPHYPYLISVE"
     gene            999..1841
                     /locus_tag="VHS06_10885"
     CDS             999..1841
                     /locus_tag="VHS06_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX2988659.1"
                     /translation="MSRIRVVTDSTADIEQEDADRLGIGVVPLNVHWNGCTYQDKVEI
                     TIDEFYRKLRQEKGTPKTSQPSVGQFEAAYRGLLEDADGIVSVHISGKLSGTLNSAQV
                     AAKSVAADRILVVDSRTTSYSLGMIVKRVASAASEGRSLRDCYELALDLVPRAKLFAA
                     VDTLEFLRRGGRIGRAQAFTGNLLAIKLVFEIRDGEVYPVDRVRTRAAVIKRTADVVL
                     DCGVLEEGAVLYGDDAEPASQLRRLIADVNPGLSLASGRIGPVLGTHTGPGVIGFAGL
                     LLRN"
     gene            1912..2757
                     /locus_tag="VHS06_10890"
     CDS             1912..2757
                     /locus_tag="VHS06_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX2988660.1"
                     /translation="MSQIRIVTDSTADMDEQLAAQLGIVTVPLKVHWNAETYEDKTEL
                     SLDEFYRKLREEKGTPKTSQPSVGRFVDVYRELLESAEGIVSIHISTKVSGTANAAQV
                     AASQVAPDRIAIVDSKNLSDALGCLAIRVARLAAGGASLPDCVATAEDLVPRLRLFAA
                     MDTLEFLRRGGRVNRMQAFAGNLLSIKPMVHLVNGEIVPVDRVRTRAASVKRMAELVA
                     ALGRLEEVAVLYGDDPRPADDLEAQLTAILPGMAVRRGRTGAVIGTHTGPGVFGASAV
                     VARGS"
     gene            2857..3144
                     /locus_tag="VHS06_10895"
     CDS             2857..3144
                     /locus_tag="VHS06_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988661.1"
                     /translation="MRLWTTAGKILAQELRTGCQDRTVIGGLEKYLSHWSSSLFGIEA
                     ASVTRPEAARVIALLSGYNVMDTQDRAVAIKMVTSVVGEVFGVPFSSRNFL"
BASE COUNT          635 a          907 c         1065 g          601 t
ORIGIN      
        1 tgaacgttca gcgggaccac gccgatgccc aacctgtcca cgtccacgtg cacacccatc
       61 agcccggcga tattcttaac caggctgccc aatacggata cctcgacaaa attaaggtcg
      121 agaacatgca aatccagcac acgtcctacc tggcaagccg cggagtacag cacacgcgct
      181 cgtccagagt gggggtggtc gtcgtggcgg ccggctctgg cttcgcaaag ctctttgaag
      241 atctcggggc ctgcgcgata gtccctggcg gccagaccat gaacccgagc atcgaggagc
      301 tcgtccacgc ggcggcgtcg tgtcagagtg agaaggtcat acttcttcca aacaatccca
      361 acgtgatcct gacggcgcag caggctcaat ccctcgctaa ggcgaacatt caggtgatac
      421 cgaccaggac gatatgcgag ggcgttgcgg ccatggttgc tttcaacgct gatgccgacc
      481 tggacgtcaa cgtcggcgga atgaaagagg ccgccgatgc cattcatacc atcgaagtca
      541 cgagagcagt caggtccacg cgagtcaacg ggctagaagt agcggagggg caggcgatcg
      601 ggttggtgga tggtaacctt gtgaatgcgg gggactcggt ggcggacgtg gcgctaatgg
      661 gcctcgacag ggtgggcgct ggcgatcacg aggttgtcac tctttatcgc ggcgacggag
      721 tgtcagaagt cgaaggccgt gatctcgccg agcgcatcac tgagcggtgg cccgatctgc
      781 aggtggatac cgtggatggc gggcagcccc actaccctta tctgatctcg gtcgagtagc
      841 gcgacacatt ccgtctctca gtctgtagga cgggcagcac gcctctccat cttctcgccg
      901 gagcgaggcg agagaacagt ccttgctctc gaccgtcgac aacctctact acttcgcccc
      961 tatcagctga ttacgactgg aaggagggct cgccaaaggt gtccagaatc cgggtggtga
     1021 ccgatagtac cgcagacatc gagcaagagg acgcggacag gttgggcatc ggcgtggtcc
     1081 cgctgaacgt tcattggaac ggctgtactt atcaggataa ggtcgagatc acgatcgacg
     1141 agttctacag gaagctcagg caggaaaagg gaactccgaa aacctctcaa ccatcagttg
     1201 ggcagttcga ggcggcgtat cgcggactgc tggaggacgc ggacggaatc gtctccgtcc
     1261 acatctccgg aaagctgagc ggaactctca actctgccca ggtcgcggct aagagcgtcg
     1321 ctgccgaccg cattctggtc gtggactcgc gcacgacgtc atactccctg gggatgatcg
     1381 tgaagcgagt cgcttcggcg gcatccgagg gccgctctct gcgggattgc tatgagctgg
     1441 ctctggatct ggtcccgcga gccaagctgt ttgccgcggt ggacacgctg gagttcctga
     1501 ggcggggtgg acggatcggc cgcgcccagg cgttcactgg caacctgctc gcgatcaagc
     1561 tggtgttcga gattcgggac ggggaagtct acccggtcga ccgggttcgg acccgagccg
     1621 ccgtgatcaa gcgtaccgcc gatgtcgtgt tggactgcgg cgtgcttgag gagggggcgg
     1681 tactgtacgg cgatgacgcc gaaccggcga gccagctccg gcgcttgatt gcggatgtga
     1741 accccggtct gagcctggcc agcggcagga ttggtccggt gctcggaacc cacacgggcc
     1801 ccggggtgat cgggttcgcc gggttgctgc tgcgaaacta gcttaacctc gggggacgaa
     1861 acgcgctcac ggtcgagggg taggagtcga gagcgcggga tggatgtgcc agtgagccag
     1921 atacgaatag ttacagacag cacagcggac atggacgagc agctggcggc ccagcttggc
     1981 atcgtaacgg tcccgctgaa agtccactgg aacgccgaaa cctacgaaga caagaccgag
     2041 ctctcccttg acgagttcta caggaagttg cgcgaggaga aggggactcc gaaaacctcg
     2101 cagccatctg tcgggcggtt cgtcgacgtg tacagggagt tgctggagag cgccgagggc
     2161 atagtctcga ttcacatttc cacgaaggtg agcggcaccg cgaacgccgc acaggtagcc
     2221 gccagccagg tggcacccga ccggatcgcg atcgtggatt cgaagaacct gtcggatgcg
     2281 ttgggatgtt tggcgattcg ggtggcgcga ctggcggcgg gaggagcttc cctgcccgat
     2341 tgcgtcgcga ccgcggagga cctcgttccc cggctgcgtc ttttcgccgc gatggacaca
     2401 ctggagtttc tgcgccgggg cggacgggtc aatcgtatgc aggcgtttgc tggaaacctg
     2461 ttgtcgatca agccgatggt gcacctggtg aatggagaga tagtgcccgt agaccgcgtg
     2521 cgaactcgtg ccgcttcagt gaagcgtatg gcagaattgg tggcggctct aggacggttg
     2581 gaagaggtcg cggtccttta cggagacgat ccgcggcccg cggacgatct cgaggctcag
     2641 ctcacggcga tcctaccggg catggcggta aggagagggc gcacgggagc cgttatcgga
     2701 actcacactg gacctggcgt gtttggtgct tcggcagtgg tggcccgcgg gagttgagcc
     2761 tcctgctcat acgcggtggt atcgaagtag catagatgtc gagtaatgac cgagaagcat
     2821 gacaatggcc cggccgcagg aggccgggcc gaattgatga gactctggac gacagccggg
     2881 aagattctgg ctcaggagct tcgtaccggc tgccaggaca ggaccgtaat cggcggtctg
     2941 gagaaatacc tctcccactg gtcttccagc ctcttcggga tagaggcggc ctctgtcacc
     3001 cgaccagagg cggcccgcgt gatcgccctg ctttctggct acaacgtaat ggacacccag
     3061 gacagggccg ttgccatcaa gatggtcacc tccgtcgtcg gcgaggtttt cggagtcccc
     3121 ttctcctcgc gcaacttcct gtagaactcg tcaagggaga gctcggtctt gtcttcgtag
     3181 gtttcggcgt tccagtggac tttcagcg
//
LOCUS       DATALS010000490         2809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_445246, whole genome shotgun sequence.
ACCESSION   DATALS010000490 DATALS010000000
VERSION     DATALS010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445246"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1235
                     /locus_tag="VHS06_10900"
     CDS             168..1235
                     /locus_tag="VHS06_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2988662.1"
                     /translation="MDDGYARLAAALDVLPNGFPRTPGGKELRILQKIFSPDDAELVS
                     HLTGQMELPTSIAARAGIAPEQVNARLKALAKRGMVWLDMDRQARSWRFRLAPFVVGI
                     YEASLELMDHELAHLVEDYMAEGGAEGIMRPEPALQRVLPARHALKTEWVLPYEDVVK
                     ILEGARSFSVRECICRVQQEQTGQRKCDFPTHNCLSFSGFGRPPGPDNISREEALDLL
                     DRCEEVGLVHTVSNVVKDVFYVCNCCGCCCGILRGVTQRGISNSVARASYLAEVDAQM
                     CTGCGACAHRCQVGAVAIVDGIATISAGRCIGCGLCVTGCTAGAATLQRRPDWETIRP
                     PETFAEWEHQRLHHREERALL"
     gene            complement(1267..1488)
                     /gene="infA"
                     /locus_tag="VHS06_10905"
     CDS             complement(1267..1488)
                     /gene="infA"
                     /locus_tag="VHS06_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX2988663.1"
                     /translation="MPKDQAIEIEGTVIEALPNATFRVQMEGGHEVLAHASGKMRKFF
                     IRILPGDRVKVELSPYDLTRGRIVYRYTK"
     gene            complement(1699..2115)
                     /locus_tag="VHS06_10910"
     CDS             complement(1699..2115)
                     /locus_tag="VHS06_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX2988664.1"
                     /translation="MADQRLLDILNRSIARELQVSIQYMWHHVQAVGLESPAVKDIFR
                     EIAIVEMKHAERFAERLDLLGGVPTTKPDPIMQGGSLQKMIEDDLKAEQEAVDMYSEA
                     VRIATEVNDPVTRLLYEEILDAEEDHRKIFGDLLKR"
     gene            complement(2336..>2809)
                     /locus_tag="VHS06_10915"
     CDS             complement(2336..>2809)
                     /locus_tag="VHS06_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX2988665.1"
                     /translation="LGIPVDIHLSYGATEAKVPEIADAVVEVTETGSTLRKNGMHIIS
                     VLLESSTQLIANHQSYADPAKRTEIEEIQTLLTGVLTARGKVLIKLNVSEENLQAVVD
                     VIPGMKSPTVSRLFGTGYYAVEAVAVKSEVNLLIPRLKAAGAEDILELPISKIVL"
BASE COUNT          526 a          828 c          853 g          602 t
ORIGIN      
        1 caactactac caactgggcg agcggcttgc gctggggcgg gtgagcaaca tgcaggagat
       61 cgtggagacg atctcgacgg cggccaaggc gatctacgtg taggttctgg gttctgggtt
      121 ctgagtggaa ttgtctcaga acccagaact gcattggagg cgggactgtg gatgacggct
      181 acgcgaggct cgcggcggca ttggacgttc tgcccaacgg ctttcctcga acgccgggcg
      241 gcaaggagct tcgaatactc cagaagatct tttcccccga cgatgctgaa ctggtcagcc
      301 acctgaccgg acagatggag ctgccaacca gtatcgcggc ccgggcaggg atcgccccgg
      361 agcaggtgaa cgctcgactg aaggcgttgg ccaagcgagg catggtgtgg ctggacatgg
      421 atcggcaggc ccgctcctgg cgattcagac ttgccccttt cgttgtcggc atctatgagg
      481 cttcgctgga gttgatggac cacgagctgg cccatttggt tgaggactac atggcggagg
      541 gcggcgcgga ggggataatg cgtcccgagc cggctctcca gcgagtcctg ccggcccgac
      601 acgccctgaa gacggagtgg gttctaccct acgaggatgt ggtgaagatc ctggagggtg
      661 cccgcagttt ctccgttcgc gagtgcattt gcagagtgca gcaggagcag acgggacagc
      721 gaaagtgtga tttccccacc cacaactgcc tatcgttctc cggtttcggc cgtccccccg
      781 gtccggacaa catctccagg gaggaggcat tagacctcct ggaccgatgc gaagaggtgg
      841 gcctggtcca caccgtcagc aacgtcgtga aggatgtctt ctacgtctgc aactgttgcg
      901 ggtgctgctg cgggatactc cgaggtgtta cgcagcgtgg gatatcgaac tccgtggcca
      961 gggcaagcta cctggcggaa gtggatgccc aaatgtgtac cggatgcggc gcttgcgcgc
     1021 accggtgcca ggtaggggcc gttgccatcg tggacgggat cgcgaccata agcgccgggc
     1081 gatgcatcgg gtgtgggttg tgcgttacag ggtgcaccgc cggtgcggct accctgcagc
     1141 gcaggcctga ttgggagacg atccgtcccc ctgagacttt cgctgagtgg gagcaccagc
     1201 gactgcatca ccgggaagaa agagcgctgc tatagcgata tagcgctgtc ccccgccctc
     1261 gatcccctac ttggtgtagc ggtatacgat ccggccacgg gtcaggtcgt aaggggatag
     1321 ctcaaccttc actcggtcgc cgggaagtat gcggatgaag aacttgcgca tcttgcccga
     1381 tgcgtgggca agcacctcgt gcccaccttc catctgaact cggaaagtgg cattcggcag
     1441 cgcctcgatg acggttccct caatctcgat tgcttgatcc ttcggcataa cgctcctttc
     1501 aagacgggca caacatgccc cgtcctttcc accctgacct tcagcgacac atccgaggta
     1561 gtttagcacg caggagccat tcctaacagt gcggatgatg acagtccagg ggcggcgaat
     1621 tcgccgcccc tgctgctttg cctcgtttcc ggtttgccac ccggcaatct acatcgatct
     1681 ccgtcgctgg ttcagccact accttttcag taggtcgccg aagatcttcc ggtggtcctc
     1741 ctcggcatcc aggatctcct cgtacagcaa cctggttacc gggtcgttga cctctgtggc
     1801 tattcggacc gcctcgctgt acatatccac cgcttcctgc tctgccttga ggtcatcctc
     1861 gatcatcttc tgcaggctgc cgccctgcat tatgggatcc ggcttcgtgg ttggtacccc
     1921 accgagcagg tccaatcgct cggcgaaccg ctccgcatgc ttcatctcta cgatggcgat
     1981 ttccctgaag atgtccttca ccgcagggct ttccagtccg actgcctgca cgtggtgcca
     2041 catgtactga atggacacct ggagttccct ggcgatggat cggttgagga tgtccagtaa
     2101 cctctggtcg gccatttgct cttcctccta agcattctcc gctgggtgtc ctgctttggc
     2161 cccgtggggc tgatcactcc acgaatctcc cgtaagaaag gagcaacctc atactacaag
     2221 catgccattg aactgtaaag tggcagtaag acgcaaacat gatgtggatg agcaacaaat
     2281 gacaaaggca ccagggtctt gcacctggtg cctttgtggt tctgtaccag cctatctaga
     2341 gaacgatctt ggagatcggc agttccagga tatcctctgc cccagccgcc ttgagccgcg
     2401 ggatcagcag gtttacctct gacttcacgg cgacggcctc cacggcatag tatccggtac
     2461 cgaagagccg agagaccgtg ggggatttca tgcctgggat cacatccacc accgcctgga
     2521 ggttctcctc gctgacgttc agcttgatca acaccttgcc acgcgcggtg agaacgccgg
     2581 ttagcagcgt ctggatctcc tcgatctccg tccgcttggc ggggtccgcg taggattggt
     2641 gattggcgat cagttgggtg gaggactcca gcagaacgga gattatgtgc atgccgttct
     2701 tgcgcaaggt ggagccggtc tcggtgacct ccacgacggc gtcggcaatc tcaggaacct
     2761 tcgcctctgt ggcgccgtag gagaggtgga tgtccaccgg aatgcccag
//
LOCUS       DATALS010000491         6146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_355459, whole genome shotgun sequence.
ACCESSION   DATALS010000491 DATALS010000000
VERSION     DATALS010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355459"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1297)
                     /gene="dgoD"
                     /locus_tag="VHS06_10920"
     CDS             complement(179..1297)
                     /gene="dgoD"
                     /locus_tag="VHS06_10920"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015842408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEX2988666.1"
                     /translation="MKITKLELLPVKPRWVFLKMHTDEGIVGYGEPVLEGRARTTMTA
                     VRELESYLIGQDPRRIEHHWQAMYRGTFYRGGPILVSAISGIEQAMWDIKGKALGLPV
                     YALLGGPARDRIRMYAHCRGETPEKSAEAASAVVAKGFTALKTSFPMSKRIIETPGYI
                     ERCVRQLGAMREAVGPEVDIAIDFFHGGYPPAMAIRLAKELEPFRPLFIEEPCLPENI
                     DAMVNIARATSIPVATGERLFTRWGFRELIEKQAAAILQPDLCHAGGIFEVRKIAAMA
                     ETYYLNLAPHCPLGPIALAACLQLDASIPNFLLQEHVTLGEGYLKQPFVVKDGYVDLP
                     TAPGLGIELDDEAVAAGAMEPNWDSPRNWFEDGAVADW"
     gene            complement(1345..2367)
                     /locus_tag="VHS06_10925"
     CDS             complement(1345..2367)
                     /locus_tag="VHS06_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2988667.1"
                     /translation="MKAVQLVAQGKLALADLPVPQPSEDEVLLRVRACGLCETDILEY
                     LLVDSIPFVHLPLVMGHEFAGEIVTLGAGVSGWQVGDRVVCESVVSCGRCAMCEAGRS
                     NLCLQRRDLGQTGDGGFAEYVAVPARNLHPLPPSLSFEEGALVEPLACVLWGLKRARL
                     QPEDDVLIIGDGFFGLMFAQLARALGAGRIVLLGHHEERMAKASEYGADVAVDERAPE
                     ASQEALRLGGGIGAPVVIDTVGSEKSLGQAISLGGVGARVALFGFSTRAAPVDPVLMV
                     LKEQDILPVLASPSVWPEVLRLLAEGKVCPQALISRVVLLEELEEAFALKAVRRPDIY
                     KIVVKP"
     gene            complement(2507..3529)
                     /locus_tag="VHS06_10930"
     CDS             complement(2507..3529)
                     /locus_tag="VHS06_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2988668.1"
                     /translation="MKAVQLVEEQKLVIGEVPTPQPRPGEVLVRVRAVGVCETDVHMY
                     FRARSMGLASLPVILGHEFSGEVAGLGAGVTDFGVGDRVVVEPVVACADCPRCRAGRP
                     NLCVNFRHLGLTEDGCFAEYVVAPARNLHRLPDHVSFEVGAMVEPLACTLWGLRRGRL
                     AAGEDILIIGDGLFGLAFAQFARALGAGRVAVLGHHQERLALAREFGADEAMDEREPG
                     VAEMAASLEGGLGPSVVVDTVGSEKSLAQAVALGGTGARIVLFGFGASTATVEPLQLV
                     FKEQDILPVLASPGVWPEVVKLLGEGKVKPQSFLSQVVPLDDLEQAFLLKASRRPDIF
                     KIVVTV"
     gene            complement(3561..4595)
                     /locus_tag="VHS06_10935"
     CDS             complement(3561..4595)
                     /locus_tag="VHS06_10935"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX2988669.1"
                     /translation="MVETDLDEREETTVSMGKMKIYATMKPAPALLDRLQSACEVSYW
                     DGEEATPHAVLLRDAAEAEATVGHYHWTAELMDKLPKLRLIANVGVGVDHVDVPAATA
                     RGIIVTNTPDVLTDTTADLTFTLLMSTARRVTEADRFIRARRWRPDGEDSTFLGRDVH
                     HATLGIVGLGRIGSAVAERGALGFKMKVLYYDVVRREDLEQKFGYQFVDLDTLLAESD
                     FVSLHMPSLPSTYHMIGAAQLAMMKPTAILVNAGRGALVDEKALIAALKEGSIAGAGL
                     DVFEQEPTDPDNPLFKMENVVVAPHIGSATAATRYGMAQLGVENVVSFALGKGPLTPF
                     NPEVLSNLKK"
     gene            complement(4727..>6146)
                     /locus_tag="VHS06_10940"
     CDS             complement(4727..>6146)
                     /locus_tag="VHS06_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX2988670.1"
                     /translation="PALSQREREPTEERVLLPLPLGEGWGEGVLGYWVRIRESRSDFP
                     DRSEAMATGSTIISVTARQVFSDRGHPGVEATVKTERGATGVAVCTAGISVGVHEVQF
                     AYDGGTRWRGKGVQRAVDSVNTVIGPALLGLDASRQREVDDAMLNLGGPDAKLRLGGN
                     ATAAVSAAVLKAGAASLGIPLYQHIGGVNACIMPVPGVITVLGSRRYGSGSRSHPKPS
                     YSLMCYGFGSFGEAAEAAWEAGTEFVQLMRKKYSLDPRQDFGVVVPPGFVQHDRELWD
                     DMTSVIGRLGYEGKVGIQVDVAAATYYEKDKDKFVGLFSREEKSKDDLVELYQSMVKN
                     WPFVIVEDPLDEVDYEGHALLTRLLGIQVVGDDLFTTNPERVQRGIELGAANTVLLKV
                     NQIGTISEAFDMVQLAYRNGYGVMPCSSRGEGADIADYTVGLGTGHLREGAVGPTGNR
                     FLKIEAELGSRAKFLGRKGLKP"
BASE COUNT         1066 a         2106 c         1896 g         1078 t
ORIGIN      
        1 tttcacctcc gagctccgcg cggcgttcag gccgttgcgc caatcgttgt acggacagca
       61 aagcaaggca gcgtaggctg gtccaggttg gacctgccgt ggatgtagcc ggcccgagtc
      121 tgcccgaccg gctacatcct catgttggac gatacacacc cgaaggaatc atgcacaatc
      181 accaatccgc cactgcgccg tcctcgaacc agttgcgggg cgagtcccag ttgggctcca
      241 tggcgccggc ggctaccgct tcgtcgtcca gctcgatgcc gagaccagga gcggtgggca
      301 ggtcgacgta accatccttg accacgaacg gctgcttcag gtagccttcg cccagcgtga
      361 cgtgctcctg cagcaggaag ttgggtatcg aggcatcgag ctgaaggcag gcggccaggg
      421 cgatcggccc cagcgggcag tgaggagcca ggttcaagta gtaggtctcg gccatagcgg
      481 cgatctttct gacctcgaag ataccaccgg cgtggcagag gtcgggctgc aggatcgccg
      541 ccgcctgctt ctcgatcagc tcgcggaagc cccaacgggt gaagaggcgc tcgccggtgg
      601 ccaccgggat cgaggtggcg cgggcgatgt tcaccatggc gtcgatgttc tcgggcaggc
      661 agggctcctc gatgaacagc gggcggaagg gctctagctc cttggccagg cggatagcca
      721 tggccggcgg gtagcctcca tggaagaagt cgatggcgat gtccacctca gggcccactg
      781 cttcccgcat tgcccccagt tggcgcacgc agcgctcgat gtagccgggt gtctcgatga
      841 tgcgcttgga catcgggaag ctagtcttga gcgcggtgaa gcccttggcg accacggcgc
      901 tggccgcctc ggcggatttc tcgggcgtct cgccccggca gtgggcgtac atacggatgc
      961 gatctcgggc gggaccgccc agcagcgcgt agacgggcag ccctagagct ttgcccttga
     1021 tgtcccacat cgcctgctcg atgccgctga tggcgctgac caggatgggc ccgccgcggt
     1081 agaaggtgcc gcggtacatg gcctgccagt ggtgctcaat gcggcgaggg tcctggccaa
     1141 tgaggtagct ctctagctcg cgcacggccg tcatcgtcgt gcgcgccctc ccctccagca
     1201 ctggctcacc gtagcctacg atcccctcgt cggtgtgcat cttcaggaag acccagcgcg
     1261 gcttgacggg cagtagctcc agcttggtga tcttcacttt ctgcactctc ctttacagac
     1321 tgacgcgatc acacccaaac aatatcacgg cttgaccacg atcttataga tgtccggcct
     1381 ccggactgcc ttgagagcaa aggcttcctc tagctcctcc agcagcacga cccgcgagat
     1441 gagcgcttgc gggcacacct ttccctcggc cagcaaccgc agcacctcgg gccagacgct
     1501 gggactggcc agcacgggca ggatgtcctg ctccttgagg accatcagca ctgggtctac
     1561 cggggcggcg cgcgtgctga agccaaagag ggcgacccgc gctcctactc cgcctagaga
     1621 aatggcctgc cccagcgatt tctcggaccc cacggtgtcg atgaccactg gcgcgccgat
     1681 gccgcctccc aggcgaagcg cctcttgact tgcctcgggc gcgcgctcgt ccacggccac
     1741 gtcagcgccg tactcgctgg ccttcgccat gcgctcttcg tgatggccaa gcaggacaat
     1801 gcgaccggcc cccagcgccc gcgcgagctg cgcgaacatc aagccaaaga agccgtcgcc
     1861 gatgatcagc acatcgtctt ccggctgcag gcgggcacgc ttcagccccc agagaacgca
     1921 ggccagcggc tccaccagcg ctccttcctc gaacgagagg ctagggggca gcggatggag
     1981 gttgcgagcg ggaacggcta cgtactccgc gaagccaccg tcgccggtct ggcccaggtc
     2041 ccgccgctgc aggcagaggt tggagcgccc ggcctcgcac atcgcacaac ggccacatga
     2101 gacgaccgat tcacacacca cccggtctcc cacctgccag ccgctcacgc cagcgcctag
     2161 cgtgactatc tcaccggcga actcgtggcc cattaccagc ggcaggtgga cgaaggggat
     2221 gctatccacc agcaggtact cgaggatatc ggtctcgcag aggccgcagg cgcgcacgcg
     2281 caacaggact tcgtcctcgg aaggctgggg aaccggcagg tccgccagcg cgagctttcc
     2341 ctgggcgacc aactgtactg ccttcataga cacctccgcg agaggagaac tgggtagctg
     2401 gacatccggc cgacgtggca ttgcagtgct cacgtcaatc ccatatgtga gttggcgacc
     2461 acctctctac cggattgacg gcaagacttc attctgccgg ctgcgtctac acggtcacca
     2521 ctatcttgaa gatatccggc cgccggctcg ccttcagcaa gaacgcctgc tccagatcgt
     2581 cgagcggcac tacctgagag aggaaagact gaggcttgac ctttccctcg cccagaagct
     2641 tcaccacttc cggccatacc cctgggctgg ccaacaccgg caggatgtcc tgctccttga
     2701 acaccagttg caggggctcg acggtagccg tgctggcgcc gaacccgaag aggacgatgc
     2761 gggcgccagt gccacccagg gccaccgcct gggcgagcga cttctccgag ccgactgtat
     2821 ccactactac cgacggcccg agacctccct ccaggctagc cgccatttcc gccacgcccg
     2881 gctcccgctc gtccatcgcc tcgtcggcgc cgaactctct cgccagcgcc aggcgctcct
     2941 gatgatggcc gaggaccgcg acgcgcccag cgccgagggc acgggcgaac tgagcgaaag
     3001 ccaggccgaa caagccatcg ccgataatga ggatgtcctc gccggcagcc aggcgtcccc
     3061 ggcgcagccc ccacagagtg caggccaagg gctccaccat cgcgccgact tcaaacgaga
     3121 cgtggtctgg taagcggtgg aggttgcgag cgggggcgac cacgtattcc gcgaagcagc
     3181 cgtcctcggt taggccaagg tgacggaagt tcacgcatag gttcggccgc ccggcgcggc
     3241 agcgcgggca gtccgcgcag gcgacgaccg gctcaacaac tacccggtct ccgaccccga
     3301 agtccgtcac tcccgccccc aggccggcga cctcgcccga gaactcgtgg ccgaggatca
     3361 cggggaggga ggccagcccc atgctgcggg cgcggaagta catgtgcacg tccgtttcgc
     3421 agacgcccac ggcccgcacc cgtaccagca cctccccggg cctgggctgc ggggtgggca
     3481 cctctccgat gaccagcttc tgctcctcga ctagctgaac cgccttcacc agacacctcc
     3541 ctccgggctt cccaatgtca ctacttcttg agattcgaca gcacttccgg gttgaacgga
     3601 gtgagtggcc ccttgcccag ggcgaacgag accacgttct ccacccccaa ctgagccatg
     3661 ccatagcgag tggccgccgt ggcgctgccg atgtgcgggg ccaccactac gttctccatt
     3721 ttgaacaggg ggttgtcagg gtcagttggc tcctgctcga agacgtcgag cccggcgccg
     3781 gcgatgctcc cctctttcag ggcggcgatg agcgccttct cgtccaccag cgcgccgcgg
     3841 ccggcgttga cgaggatagc ggtgggcttc atcatggcta gctgggcggc cccgatcatg
     3901 tggtaggtgg agggcaggct tggcatatgc agggagacga agtcggactc tgccagcagg
     3961 gtgtccaggt cgacgaactg gtagccgaac ttctgctcca ggtcttcgcg gcgcaccacg
     4021 tcgtagtaga gcaccttcat cttgaagccc agtgccccgc gctcggccac ggcagagccg
     4081 atgcggccca ggccgacgat gcccagcgtg gcgtgatgca cgtcgcgccc gaggaacgtg
     4141 gagtcttcac catccggtcg ccagcgtcgg gcgcggatga agcgatcggc ttccgtgacg
     4201 cggcgggccg tcgacatcag taaagtgaag gtgaggtccg cggtggtatc ggtcagcacg
     4261 tccggcgtgt tggtgacgat gatcccacga gcggtggccg ccggcacgtc cacgtggtcc
     4321 acgccgacgc ccacgttggc aattagacgt agcttgggca gcttatccat tagctcggcc
     4381 gtccagtgat agtgccctac cgtggcttcc gcctcggcgg cgtcacgcag cagcaccgcg
     4441 tgcggggtcg cctcttcccc atcccagtag ctgacttcgc aggcgctctg cagtcgatcc
     4501 agcagcgccg gcgccggctt catcgtcgcg tagatcttca tcttgcccat gctgactgtt
     4561 gtctcctctc gttcatccag atctgtctcg accacgctcg gttgtcttct ggtagctgac
     4621 gtcccctgtc atgctgagcc gaaggcgaag catccgtctc tccttgcctc cagacgcgcc
     4681 ggagcggatt cttcggcctg cgggcctcag aatgaccgag tgggttctac ggcttcagcc
     4741 ctttccttcc caggaacttg gctcggctgc ctagctcggc ttctatcttc agaaaccggt
     4801 tgcccgtggg ccctaccgct ccctcgcgca agtggccggt gccgaggccg acggtgtagt
     4861 cggcgatgtc agccccctca ccccggctgc tgcacggcat gacaccataa ccattgcggt
     4921 aagctagctg caccatgtcg aaggcctcgc tgatggtgcc gatctggttc accttcagca
     4981 gtaccgtgtt ggccgccccc agctcgatgc cgcgttgcac ccgctccggg ttggtggtga
     5041 agagatcatc gcccacgacc tgaatgccaa gcagccgcgt cagcagagca tgcccttcgt
     5101 agtccacctc atccagcggg tcctcgacga tgacgaaggg ccagttcttc accatcgact
     5161 ggtatagctc aaccaggtcg tccttggact tttcttccct ggagaagagg ccgacaaact
     5221 tgtccttatc cttctcatag taggtggcgg ccgccacgtc tacctggatg ccgaccttgc
     5281 cctcatagcc caggcggccg atgaccgagg tcatatcgtc ccatagctcg cggtcgtgct
     5341 ggacgaagcc tggcggcacc acgaccccga aatcctgtcg cgggtcgagg ctatacttct
     5401 tacgcattag ctggacgaac tccgtgcccg cttcccaggc cgcctcggcg gcctcgccga
     5461 agctgccgaa gccgtagcac atcagagagt aactgggctt ggggtgagag cgacttccac
     5521 tcccgtagcg gcgactgccc agcactgtga tgacgccggg cactggcata atgcaggcgt
     5581 tgacgccgcc gatgtgctgg taaaggggaa tgccgaggct ggcggccccg gccttgagca
     5641 ccgcggccga gaccgccgcc gtggcgttgc cgcccagccg cagcttagcg tctggcccac
     5701 ccagattgag catcgcgtcg tccacctcgc gctgccggct ggcatccagg ccaagcaggg
     5761 cggggccaat gacggtgttg acgctatcca cggcccgctg cacgcccttg ccgcgccacc
     5821 tggtcccacc gtcgtaggcg aactggacct cgtggacgcc caccgagatg ccggctgtgc
     5881 aaacggccac gcctgtggcg ccgcgctcag tcttcacggt ggcctcgacg cctgggtgcc
     5941 cacgatcgga gaagacctgc cgcgccgtta ccgatatgat agtgctgccc gttgccatgg
     6001 cttcactcct atctgggaaa tcgcttcggg attctctgat gcgaacccaa taacccagga
     6061 caccctcacc ccagccctct cccaaaggga gagggagtag gacgcgctcc tccgtaggct
     6121 ccctctccct ttgggagagg gctggg
//
LOCUS       DATALS010000492        11672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_308059, whole genome shotgun sequence.
ACCESSION   DATALS010000492 DATALS010000000
VERSION     DATALS010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 11672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11672
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308059"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2369
                     /locus_tag="VHS06_10945"
     CDS             <1..2369
                     /locus_tag="VHS06_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2988671.1"
                     /translation="VPQIVADVERDHRAFHVLGTPVLDESMIAAPINFEGRVIGVITL
                     SRLGLGQFSVDDLRLLDIFANEAAIEFENARLYQETQKKSKELLASFHRVGDALATGL
                     DPNQTLQIIVDLAAEMVRAKACAMLLVDENDGELIFSAARGLPVKVEQGARVPAGRGV
                     SWQVVKEGVAKMASDVSAVERTPWMRGEGIEDLHSYLGVPLMMRGRAIGVLSVFGTRV
                     GQFSQADVELLSSFAHQAAIAIRNAQLFTSLQERVRELTGLAEVSRSVVTLTNLDDTY
                     RELTKSIAQLLQAEDCVILLAGENGELSPQFPAYGFTVEEMEGVSLRVIEGGNLAVDP
                     LCPFQAVDGLTVSCAGNWGRQPSLVAPMRAQDRLVGALLVSGRPESVFNENDLRLLTI
                     LSANAAVIVQNAILYQSVDRERDELDAIISNTSDAIFIVDQENRITRINAAAEALMGW
                     RADEVMGRECDETVFGCPTAGTSVSDTVPSLREVVEKREPIPYFETMVVTKDGRERDV
                     AASYSFVHSESRGQGLGVVIARDISKQREVDRMKSEFVSMVSHELRTPLGLIKGYAST
                     LLNPQLNLDAATVQRFLMGIDGAADRLARLIENVLSVSRIESGLLRISAQPVDLSHLV
                     SAAVTNARATTKGHEIQLTLPRRPVKVEGDKIQLELVMDNLLGNAIKYSPMGRPIEVQ
                     VESRGSEVEVRVADQGIGIAAHHLPKVFDKFYRVEGGYSRRTPGSGLGLFICRNIIEA
                     HGGRIWAESVLGEGSTFAFVLPTSQPERAGDAEGVGSRSERGEGKGDE"
     gene            2362..2817
                     /locus_tag="VHS06_10950"
     CDS             2362..2817
                     /locus_tag="VHS06_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2988672.1"
                     /translation="MSRNRILVVDDEPGIVDIAKANLEGQGYQVIEAYDGENALLRIK
                     EEKPDLVVLDILMPEMDGWDVLEQIEADPELSGIPVIMLTARVSDEDVLRGLETGAVE
                     YMTKPFYPQDLVAAVKINLHVFDPQLRQQHRRLLAEKRRRLMAARGHAM"
     gene            2849..3238
                     /locus_tag="VHS06_10955"
     CDS             2849..3238
                     /locus_tag="VHS06_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2988673.1"
                     /translation="MTQSKVVVVEDEPEMLEIVKINLEQAGYEVFPAHDGLEGYELLE
                     EKDPDALILDLGLPNLSGFRLLRLLRRDPRWSHLPVIVATAYAFEEVEDLANEGIDGF
                     ISKPFDPADLVSRLQFVLARGRRTAAG"
     gene            complement(3247..3633)
                     /locus_tag="VHS06_10960"
     CDS             complement(3247..3633)
                     /locus_tag="VHS06_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2988674.1"
                     /translation="MANSDQSEAFCPYYRHAVELVGRRWSGTIIRALAGGVHRFSDIV
                     DTIPGLSDRMLSERLKELETEGIVVRTVYPETPVRIEYHLTEKGRALGGVMDALSDWA
                     HRWLAPSAEETGHLSEDPHREKQRSC"
     gene            3842..4399
                     /locus_tag="VHS06_10965"
     CDS             3842..4399
                     /locus_tag="VHS06_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX2988675.1"
                     /translation="MSNVIEEKPVVSSWNVDPAHSSVEFAVKHMVITTVRGRFAKFDV
                     DLNFDEANPLQSSVVAKIDAASIDTREDKRDGHLRSADFLDAENHPFLVFTSKRVEDA
                     GGGRYQIVGDLTIRGVTREVSLDTTFSGFAKSPWGMTVAGFNAETTINRADWGLVWNA
                     ALETGGVLVAEDVKIAIEVEAIKQG"
     gene            complement(4445..5422)
                     /locus_tag="VHS06_10970"
     CDS             complement(4445..5422)
                     /locus_tag="VHS06_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX2988676.1"
                     /translation="MTTITEMMKGARTLCGPCTNVRAGESVLVVTDVGMVPIAEAVAA
                     AAKERGAEAVIAIIDQRASDSTEPPKPVAEAMKSSSVVFTPVSISITHTRAVKDAVAA
                     GARAIALTAVTPEMMVSGGLDADFEQVKVTCRAVGARMAKAKTARVTNSFGTDLTFRV
                     EGRRVNVMPCVVEPGEFSPAPTAEVNFSPIEGSANGIIVADASIPYLGIGLLKSPIRF
                     TVRDGFIVSVEGGDREQVATLKTAWEKMADPNVYNVAEMGIGMNPMCRFTGIMLSDEG
                     VQGSIHIGTGTNITLGGTVKAKCHYDLIMAKPTLELDDQLLIQNGALML"
     gene            complement(5457..6716)
                     /gene="larA"
                     /locus_tag="VHS06_10975"
     CDS             complement(5457..6716)
                     /gene="larA"
                     /locus_tag="VHS06_10975"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX2988677.1"
                     /translation="MVDISVPYGRTQLTFRIPEAASLSVVEPSSAPAIPDPAAVLARA
                     LDVALARRPFPPVHDAVVVVSDGTRPVPNGTILPPLLSRMEAAGIPREGITILVGTGL
                     HRPTREAELPALLGPGICARYRVAVHDARDREQLAYLGQTSRGTPIWINRSYVNSRLR
                     VLTGMIEPHQFVGFTGGAKSVAIGLAGAETIAGNHALLQDPRSQIGVYEGNPAREEME
                     EILESVGADLAVNVILNNDKEIVELLAGPAREVQRTGVVHSAELCQVPLHHPFDIVIA
                     SPGGFPKDREMYQAQKAVAHAAIAVREGGTIILVAECPEGSGEDRFEEWMSAASDPSD
                     VLERFRREGFQMGAHKAFLLCRSMVKARLLLVSDRMPADLARRLLLELHRTTESALAA
                     ALAYHGENASIAVMPKASSTIPRQELR"
     gene            6918..7361
                     /locus_tag="VHS06_10980"
     CDS             6918..7361
                     /locus_tag="VHS06_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX2988678.1"
                     /translation="MGLSEQLMEDLKDAMRKHDEIRVSTIRFARAAITNEQIEKRRPL
                     EESEIVGVLAKQVKQRRESIEEFQKGNRPDLVAKEQAEMEILLSYLPKQMTREEIEDA
                     ARATIQEVGATSANDFGKVMSRLAPQLRGRADGKVIGEVVRGLLH"
     gene            7509..9680
                     /locus_tag="VHS06_10985"
     CDS             7509..9680
                     /locus_tag="VHS06_10985"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX2988679.1"
                     /translation="MALRDAVTARERPQLDGHRITRLILLAAALAVGLAFILLVPLRF
                     SSYGLHEGDVAPTNIRAPKHVQYVSAIETKAEKERAANSVAEQFAFDDKVASQQRGKM
                     VVGLQSIAFTRANTGLTPDARKNAMRKSFVDFDLTDDQIAVLLAMDNARWQVFSSDAP
                     RVLYDLLAEKVPAERLKSLQRDAWTKLGNLSDPDRDLAADLVQRLSKVNYIPNPAETE
                     RLRKDAQDAVAPVTKTVEQSEIILREGDIVRATDLEKLEALGLRNPTVDWRAVASSSL
                     LALIVVVLLVGYLWVFDPSLLIQEKRLLLLILVMLVTIVAAKAVLPGRPFWSYIFPIS
                     AVAMLLATLLDAQLGIVVTMLLSVLVAMVAGNSLEVAFIGIVGAVLGTMGVRRSDRLH
                     SYFVAGGMVALGSFAVIAVFRLAAKDDDWMGLLLMGGLSVVNGLLAASLTVGTFSLLG
                     RIFGITTTMQLLELSDPTQPLLRRLLNEAPGTYHHSIMVGNLAERAAERVGADALLVR
                     VGAYYHDVGKLARPYFFIENQFDGKNVHDSLDPQTSARVVLSHVRDGLDLAEKYKLPP
                     RVREFISEHHGTRLATYFFNQATRDGDGRVDASQYAYPGPRPRSKEAAIVMLADSVEA
                     VVRSVKDHSPDNVATLVRKVVDERVDEGQLDDCDLTMRDVDEIKRAFIAILMGMYHPR
                     IEYPPAAITSTPVTPSILTSSERVLVSGEDGNGEGDLDEHA"
     gene            9667..10158
                     /gene="ybeY"
                     /locus_tag="VHS06_10990"
     CDS             9667..10158
                     /gene="ybeY"
                     /locus_tag="VHS06_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX2988680.1"
                     /translation="MSMPDVVVEWEVEEPLEGKVNRGFLEGVVREALAGRNLNGLLAV
                     GLTITDDEGIRELNRDHRGLDSATDVLSFPLQQYDAPEKPSIQFPQPPGEPLPLGDIV
                     VSYPRAEEQAHSYGHSLDRELAFLVVHGIMHLMGYDHEVATDAEKMRAEEEAVLGRLG
                     LTR"
     gene            10295..10666
                     /locus_tag="VHS06_10995"
     CDS             10295..10666
                     /locus_tag="VHS06_10995"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2988681.1"
                     /translation="MSRSLYHSFANAFHGLSYALRYERNPQIHLAMALATVAVGLWLG
                     LAPFEWAILVLTISAVLATELLNSALETLADATSPEYHPLVGIAKDVAAGAVLVIAIG
                     AVAVGLLLFLPKLIAILDNIS"
     gene            complement(10751..>11672)
                     /locus_tag="VHS06_11000"
     CDS             complement(10751..>11672)
                     /locus_tag="VHS06_11000"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX2988682.1"
                     /translation="EKIVDTSDEWIVSRSGISERRIISEDETTSTIAAAAARQAMERA
                     GVGAQDIDLIILATITGDYPNVPASASLVQNILGIKCCGAFDLVAGCSGFVCGLVTGS
                     QFIQNGACRNVLVIGAESMTRVLDWTDRTTCVLFGDGAGAVVLRPTEKEGGLLSFVLG
                     SDGAGAEHLIIPAGGTKAPATHDTVERRQHYVKMNGKEVFKFASRILPRAFHQTLEKA
                     GIRPEEVSLLVPHQANIRIIESARQNLAVPEDAIYVNVNRYGNTSSASIPVALCEAIE
                     EGRLKPGSILAMVAFGAGLTWGSVAWRWQG"
BASE COUNT         2176 a         3353 c         3803 g         2340 t
ORIGIN      
        1 gcgtgccgca gatcgtggcg gatgtggaga gggatcacag ggcgttccat gtgctcggga
       61 caccggtact ggacgagtcg atgatcgccg cccccatcaa cttcgagggg cgagtcatcg
      121 gtgtgataac cctgtcgagg cttgggcttg ggcagttcag tgtggatgac ctgaggttac
      181 tggacatttt tgccaacgag gcggcgatcg agttcgagaa tgcgaggttg taccaggaga
      241 cccagaagaa gagcaaggag ttgttggcct ctttccaccg agtcggagat gcccttgcca
      301 ctgggctgga cccgaaccag acgctgcaga ttatcgtgga cctggcggcc gagatggtgc
      361 gagccaaggc ctgcgcaatg ctgctcgtgg acgagaacga tggagagttg atcttcagcg
      421 cggcaagagg gctccccgtc aaggtggagc agggggccag ggtcccggcc ggtaggggcg
      481 tgtcctggca ggttgtcaaa gagggcgtcg cgaagatggc gtcggatgtc tcggcggtcg
      541 aacggacacc atggatgcgc ggcgaaggga ttgaggatct ccattcctac ctcggtgtgc
      601 cgctgatgat gcggggccgg gccatcgggg tgctgtcggt cttcggtact cgcgtgggac
      661 aattcagcca ggcggacgtg gaactgcttt cctccttcgc ccaccaggca gccatagcga
      721 ttcgcaatgc tcagctcttc acgtcgctgc aggagcgggt tagggagttg actgggctgg
      781 ctgaggtctc ccggagcgtg gtcaccctga ccaacctgga tgacacatat cgcgagttga
      841 caaaatccat cgctcaactg cttcaggcgg aagactgtgt gatcttgctg gccggtgaga
      901 acggagagct gtcgccgcag ttccctgcct atggcttcac ggtggaggag atggaaggtg
      961 tgagcctgcg ggtgatagag gggggaaacc tggctgtaga tcccctctgc ccgttccagg
     1021 ccgtggacgg gctgacggtt agttgcgcgg ggaactgggg ccggcagcct tctctcgttg
     1081 cacccatgag ggctcaggat cggctcgtcg gcgcgttgct ggtaagcggt cgcccggagt
     1141 ctgtgttcaa cgagaacgac ctgcgcctgc tcaccatcct ctcggccaac gctgccgtta
     1201 tcgtgcagaa tgccatcctg taccagagcg tcgatcggga gagggatgag ctggatgcca
     1261 tcatcagcaa cacatctgac gccatattca tcgtggatca ggagaaccgc atcacccgga
     1321 taaatgcggc cgccgaggct ttgatgggct ggcgtgccga tgaggtgatg ggacgcgagt
     1381 gtgacgagac ggtcttcggc tgtcctaccg ccggcacaag cgtgagcgac actgtgccct
     1441 ctctgcgaga ggtcgtggag aagagggaac ccatacccta tttcgagacg atggtggtga
     1501 ctaaggacgg tcgggagagg gacgtggcag ccagctattc cttcgtccat tccgagtccc
     1561 gaggtcaggg actgggggtc gttattgccc gggacatcag caagcagcgc gaggtagacc
     1621 ggatgaagtc ggaattcgtc tccatggtct cccacgagct gaggaccccg cttggactga
     1681 tcaagggcta tgcctcgacg ctgttgaacc ctcaactgaa tctggatgcc gctacagttc
     1741 agcgtttcct gatggggatc gacggcgcgg cagaccggct ggccaggttg atcgagaacg
     1801 tgctcagcgt gtccaggatc gaatctggac tgcttcgaat ctccgcgcag cctgtggatc
     1861 tttcgcacct agtgtccgca gcggtgacca acgccagggc caccaccaaa ggacacgaaa
     1921 tccagttgac cctgccccgc cgaccggtga aggtggaggg ggacaagatc caactggagc
     1981 tggtgatgga taacctgctg ggcaacgcca taaagtactc gccgatgggc aggccgatcg
     2041 aggttcaggt ggagagccgt ggctcggagg tggaggtccg ggtcgccgac cagggaatag
     2101 gaattgctgc tcaccatttg cccaaggtat ttgataagtt ctacagggtg gagggcggat
     2161 acagccggcg aacgccgggc agcggtcttg gtctcttcat ctgccgcaat atcatcgagg
     2221 cccatggggg ccgcatctgg gcagagagcg tgttgggaga gggaagcact ttcgcctttg
     2281 tccttcccac ttcgcagccc gaacgggctg gggacgctga gggcgttggc tcgaggtcgg
     2341 aacgaggaga ggggaaaggg gatgagtaga aaccgcattc ttgttgttga tgacgaaccg
     2401 ggcatcgtag atattgccaa ggcgaacctg gagggccagg gataccaggt gatcgaggcg
     2461 tacgacgggg aaaatgcgtt actgaggatc aaggaggaga agccggacct ggtcgtgctc
     2521 gatatcctga tgccagagat ggacggatgg gatgtgctgg agcagatcga ggcggacccg
     2581 gagctttcgg gtattcccgt tatcatgctc acggcaaggg tgagcgatga ggatgtcttg
     2641 cgtgggctgg agaccggtgc cgtcgagtat atgacgaagc cgttctaccc tcaagatctg
     2701 gtggcggcgg tgaagatcaa ccttcacgtg ttcgacccgc agctgcgtca gcagcaccgc
     2761 aggctgttgg ccgagaagag gcgcaggttg atggcggcta ggggccacgc tatgtagcgt
     2821 tctaatactg gtcgggcggg tagtggcaat gacgcaatcg aaagtggtgg tagtagaaga
     2881 cgagcccgag atgctggaga tagtgaagat caatcttgag caggcgggct acgaggtgtt
     2941 tcctgcccat gacgggctgg agggctatga gcttctggag gagaaggatc cagatgccct
     3001 catactggat ctggggctcc ccaacctgtc gggttttcgg cttctgaggt tgctccgacg
     3061 cgacccgcgc tggtcgcatc ttcccgtaat cgtggccacg gcttatgcct tcgaggaggt
     3121 ggaggacctg gccaacgagg gcatagatgg cttcataagc aagcccttcg atccggccga
     3181 cctggttagc cgcctgcagt tcgtcctggc caggggacgc cgcacggccg cgggctgagc
     3241 cccgcatcaa caggagcgtt gcttctccct atgtggatcc tcagacaagt ggccggtttc
     3301 ttcggcggaa ggggccagcc accggtgcgc ccagtccgag agagcatcca tgacgcctcc
     3361 cagggcccga cccttttcgg tcaggtggta ctcgatcctt accggcgtct ccggatacac
     3421 ggtgcggaca actattccct ccgtttccag ttccttcagc ctttcagaga gcatgcggtc
     3481 gctgagacct gggatcgtat cgacgatatc gctgaaacga tggacgccgc cggccaatgc
     3541 ccggatgatc gtgcccgacc accgccgtcc gaccaactcc accgcgtgcc tgtagtaagg
     3601 gcaaaaggct tcggattgat ctgagttcgc catctcgtac cttcgacttc ccgcggcagt
     3661 cggccgccat cgcttactat tcgtgagtat cctcctgtcc ataattatac ggcatgggag
     3721 agatacttga caaggctaac tactggtcgt atagttactt ctgcaaggta agtaaccggt
     3781 cgagggtaag cgcctcgtcc gagaatctag atcaggaacc ctttgggagg gatagaatcc
     3841 aatgtccaac gtaatcgaag agaagcccgt agtttccagt tggaacgtcg atccggcgca
     3901 ctccagtgtc gagtttgccg ttaagcacat ggtcatcacc actgttcggg gccgtttcgc
     3961 aaagttcgac gtcgatctca acttcgacga ggcaaacccg ctgcagtcca gcgtagtagc
     4021 caagatcgat gccgccagca tcgatacccg tgaagacaag cgcgacggtc acctgcgctc
     4081 cgccgatttc ctggatgccg agaaccatcc attcctcgtc ttcaccagca agagggtcga
     4141 agacgccggc ggcggccgct accagattgt tggggatctg accattcgtg gcgtcacccg
     4201 ggaggtttcc ctcgatacca ccttctcggg cttcgccaag agcccctggg gcatgacggt
     4261 ggccggcttt aacgccgaga cgaccatcaa ccgtgccgac tgggggctgg tctggaatgc
     4321 cgcgctggag accggtgggg ttctggtggc cgaggatgtg aagatcgcca tcgaggtcga
     4381 ggccatcaag caggggtaag gcatctgaga ggggccggca ggagcgccgg cccctcggcc
     4441 ctttctacag catcaacgcg ccgttctgga tcaacagttg gtcgtccagt tcgagcgtcg
     4501 gcttcgccat tatcaggtcg tagtggcact tggccttcac ggtgcctccc agggtgatgt
     4561 tggtgccggt gccgatgtgg atgctgccct gcaccccctc gtccgagagc atgatccctg
     4621 tgaagcgaca catcgggttc attccgatgc ccatctcggc cacgttgtac acgttggggt
     4681 ccgccatctt ctcccaggcc gtcttcaagg ttgccacctg ctcccgatcg cccccttcga
     4741 cggagacgat gaaaccgtcg cgaaccgtga agcggatggg cgacttcagc aacccgatcc
     4801 caagataggg gatgctggca tcggcaacga ttataccgtt ggcgctgccc tcgatcgggg
     4861 agaagttcac ctcagcggtg ggcgccggtg agaactctcc aggctcgacc acgcatggca
     4921 tcacgttgac tcgccggccc tcgacccgga acgttaggtc ggtgccgaag ctgttggtca
     4981 cccgggcggt cttcgctttc gccattcggg caccaaccgc ccggcatgtt accttcacct
     5041 gctcgaagtc cgcgtccagt ccgccagaga ccatcatctc gggggtcacc gccgttaggg
     5101 cgatagccct ggccccggca gcgaccgcat ctttcaccgc tcgggtgtgt gttatggata
     5161 tgctgacagg ggtgaacacc acgcttgagg acttcatcgc ctcggcgacc ggctttggcg
     5221 gctccgtgga gtcggatgcg cgctggtcga tgatggcgat gaccgcctcg gcaccccgct
     5281 ccttggccgc cgcggctacc gcctcggcta tgggaaccat tcccacgtcc gttaccacca
     5341 ggacgctctc gcctgccctg acgttggtgc agggaccaca gagcgtccgg gcgcccttca
     5401 tcatctcggt aatcgtagtc aacgcagtct cctctactag tgttgtggcg cagccgtcac
     5461 ctaagttctt gtcttgggat agtggacgac gccttgggca tcaccgcgat gctcgcgttc
     5521 tcgccgtggt aggccagcgc cgcggccagg gcggattccg tggtgcgatg tagctccagc
     5581 agtagccgcc gtgcaagatc tgccggcatt cggtccgaga ccagcagcag ccttgccttc
     5641 accatgctgc ggcacagcag gaacgccttg tgggcgccca tctggaatcc ctctcgtcta
     5701 aatcgctcca gcacgtcgga cggatctgag gcggccgaca tccactcctc gaacctgtcc
     5761 tcccctgatc cctcggggca ttcggccacc aggatgatgg tgcctccctc cctgaccgct
     5821 attgcggcat gcgccaccgc cttctgggcc tggtacatct ctcggtcctt gggaaaacca
     5881 cctggggagg cgatcacgat gtcgaagggg tggtgcagcg gaacctggca tagctcggcg
     5941 gagtggacta ccccggtgcg ctgaacctcc cgcgctggac cggccagcag ttccacgatc
     6001 tccttatcgt tgttcaggat cacgttcacg gccaggtcgg cgcccacaga ctccaggatc
     6061 tcctccatct cctcccgggc agggttgccc tcgtacactc cgatctggga gcgggggtcc
     6121 tggagcagcg cgtggttgcc ggcgatggtc tcggctccag ccagcccgat ggcgacactc
     6181 ttggcccctc cggtgaagcc gacgaactgg tgtggctcta tcatgcctgt gaggactcga
     6241 agccgactgt tcacatagct tctgttgatc cagatggggg tacccctgga ggtttgcccc
     6301 agataggcca actgctccct gtcgcgtgca tcgtggacgg ctaccctgta tcgggcgcag
     6361 attccgggtc cgaggagggc aggcagttct gcctctctag ttgggcggtg caggccggtg
     6421 cccacgagga tggtgatccc ttcccttggg atacctgcgg cctccatgcg gctcaacaga
     6481 ggaggtagga tcgtcccgtt gggaacgggg cgtgttccgt cgcttactac cactaccgca
     6541 tcgtgaacgg gcgggaacgg acgtctggcc agtgccacgt ccagcgcgcg ggcgagtacc
     6601 gccgccgggt cgggaatggc cggcgcggag gaaggctcta ccaccgacag agatgcggcc
     6661 tcgggaatgc ggaaggtgag ctgggtcctg ccgtagggca cgctgatgtc gaccacaatg
     6721 cgctccttcc tcaacaagga tggcagcggg atcatagcac tagtgttgtg tcaacgacga
     6781 atcctctgat ggtaggggcg tatctatagg gaatgatgga taccagcaag agaaagttgc
     6841 ttagatcctg tgggcaaacc ttgacactcc agaaaaccga aacctataat ccgtttagtc
     6901 ataggaggcg ggcagagatg ggtttgagtg agcagttgat ggaagacctg aaggatgcca
     6961 tgcgcaagca tgatgagatt cgggtttcca ccattcgttt tgccagagcg gccataacca
     7021 acgagcagat agagaagcgc aggcctttgg aggaatccga gatcgtcggc gttcttgcca
     7081 agcaggtcaa gcagcgccgc gagtcgatcg aggagttcca aaagggcaat cggccggacc
     7141 tggtggccaa ggagcaggcg gagatggaga tcctcttgag ctacctgccc aagcagatga
     7201 cccgagagga gatcgaggac gcggccaggg ctaccatcca ggaggtaggg gccacgagcg
     7261 ccaatgactt cggcaaggtc atgtccaggc tggctcccca gttgagggga cgggccgatg
     7321 gcaaggtcat cggcgaggtg gtgcgcgggc tgctgcatta gagtagtcac tgtgcgggca
     7381 ctccggtgcg attctgtcga ccgggtgcct gttctgttac ggggcgactt cacctgtcag
     7441 gtgccaccgg cagtgcggca gcgcaaaatg tcggcaccaa attctggccc aggagaggcg
     7501 attcgaaagt ggcactgcga gacgcggtga ctgcccgtga gcgcccacag ttagacggcc
     7561 acaggatcac tcgtctgatc ctgcttgcgg ccgcgctggc tgtagggctc gcctttatcc
     7621 ttctggtccc tctgcgattt agcagctacg ggctccatga gggcgacgtg gctcccacca
     7681 atatacgtgc ccccaagcac gttcagtatg tctccgccat cgaaaccaag gccgagaagg
     7741 agcgcgccgc taacagcgtt gccgagcagt ttgctttcga tgacaaagtg gcatcccaac
     7801 aacgcggcaa gatggtcgtg gggcttcaat cgatcgcgtt tacccgtgcc aataccggtc
     7861 tcacccctga tgccaggaag aacgcgatgc ggaagtcgtt cgtcgatttc gaccttaccg
     7921 acgaccagat cgccgtgctg ttggcgatgg acaatgcccg ctggcaggtt ttctccagcg
     7981 atgcaccgcg agtgctctac gatttgctgg cagagaaggt cccggcggag cggctgaaga
     8041 gtctgcagag ggatgcatgg accaaactgg gcaacctatc ggatccggac cgcgacctgg
     8101 cagcggatct ggtgcagcgg ttgtcgaagg taaactacat tcccaatccg gctgagacgg
     8161 agcggctgcg caaggatgcc caggacgctg tggcgccggt gactaagacc gttgagcaga
     8221 gcgagatcat cctacgggag ggtgatatcg ttcgggcaac cgatctggag aaactggagg
     8281 cgctggggct gcgcaacccg acggtggact ggcgcgcggt cgcttcctct tccctgctgg
     8341 cgctgatcgt cgttgtgctt ctggtcggct atctgtgggt cttcgatccc tcactgctga
     8401 tccaagagaa gaggttgctg ctgctgatcc tcgtcatgct ggtgaccata gtcgcggcca
     8461 aggcggtgct gccgggccgg cccttctggt cctacatctt ccccatttcc gcggtggcta
     8521 tgttgctggc caccctgctg gacgcgcagt tgggtatagt ggtgactatg ctgctgagcg
     8581 tactggtggc gatggtcgcc ggcaactccc tcgaggtggc attcataggt atagtaggcg
     8641 cagtattggg cacgatgggt gtgcgccgaa gcgaccggtt gcactcctac ttcgttgccg
     8701 gcggcatggt tgccctgggc agcttcgctg tgatcgcagt gtttcgactt gccgcgaagg
     8761 acgatgactg gatgggactg ctgctcatgg ggggcctgtc cgttgtgaac ggtttactgg
     8821 cggcgtctct gacggtcggg accttcagcc tgctgggacg catctttgga ataaccacca
     8881 ctatgcagct acttgagctt tcggacccca cccagccgct gcttcggcgg ttgctgaacg
     8941 aggcgccggg cacctatcac cacagcatta tggtcggcaa cctggccgag cgtgccgcgg
     9001 agcgagtcgg ggccgatgcc ctgttggtgc gggtcggggc ttactatcac gatgtcggta
     9061 agctggcgcg accctacttc ttcatcgaga atcagttcga tggaaagaac gtccacgact
     9121 cgctggatcc ccagaccagc gcccgcgtcg tgctttcgca cgttcgagat ggtctagacc
     9181 tggctgagaa gtacaaactc ccgcccaggg tacgggagtt catttcggag caccacggca
     9241 cgaggctggc aacttacttc ttcaaccagg ccacccggga tggggacggc cgggtggatg
     9301 cctcacagta cgcctacccc ggccccagac cccgaagtaa ggaagcggcc atagtcatgc
     9361 tggccgactc ggtggaggcg gtcgtgcggt cagtcaagga tcactctccc gataatgtcg
     9421 cgacgctggt gcgaaaggtt gtggacgaaa gggtcgatga gggacagctg gacgactgcg
     9481 atttgactat gcgggacgtg gatgagatca agagggcctt catcgccatc ctgatgggca
     9541 tgtatcaccc ccggatcgag tatccaccgg cggcaatcac ttcgactccc gtcaccccat
     9601 ccattctcac gtcgagcgag cgggtgctgg tgtcgggcga ggacggtaat ggggagggcg
     9661 atctggatga gcatgcctga cgtcgttgtc gagtgggagg tggaggagcc tctggagggg
     9721 aaggtcaacc gcggcttcct cgagggcgtg gtgagagaag cgctcgctgg aaggaatctg
     9781 aatgggctac tggctgtcgg tttgaccata accgacgacg aaggcattag ggagttgaac
     9841 agggatcatc gggggctgga tagtgccacc gatgtcctat cctttccttt gcagcagtac
     9901 gatgctcctg agaagccgtc gatccagttt ccgcagccgc cgggagagcc gctgcctctg
     9961 ggggatatcg tcgtgtccta cccccgagcc gaagagcagg cacacagcta cggacacagc
    10021 ctcgatcggg aactggcgtt cctggtggtc cacgggatca tgcacctgat ggggtacgat
    10081 cacgaggtcg ccacggatgc ggagaagatg cgggctgaag aagaggcggt gctggggcgg
    10141 ctgggcctca cgcggtgacg ggtgccgagt tctgggttct gggtgctgag attcccctct
    10201 ccctctggga gaggcgttga catgccagag ggcatgttgt ggtaagagcg agcctatctt
    10261 gacactggct tcggcaggag gctgcctcca atagatgtcc cgtagtctct atcactcctt
    10321 cgcaaatgct ttccacggac tctcctacgc attgcgctac gagcgcaacc cgcagatcca
    10381 cctcgctatg gcccttgcga cggtggctgt tgggttatgg ctggggctgg ctcccttcga
    10441 gtgggcgatc ctcgttctca ccatttccgc ggtgctggcg accgagttgc tgaacagcgc
    10501 ccttgagacg ttggcggatg cgacctcgcc ggagtatcac cccctggtgg ggatagccaa
    10561 ggacgtcgcg gccggggcgg tgttggtgat agcgatcggg gctgtggcgg tggggttgct
    10621 gctgttcctg ccgaagctaa ttgcgatcct ggacaatatc tcgtagggcg ggtcgggggc
    10681 gacccgccct actggaaaag ggccagcctt agctcgctcg cgctgcgggg gtgacccgcc
    10741 ctaccagata ctagccttgc cagcgccagg cgacggagcc ccaggtgagg ccggccccga
    10801 aagccaccat cgccaggatg gatcccggct tcagccttcc ttcctcgatt gcctcgcaca
    10861 gggccacagg gatcgatgcc gaggaggtat tgccatagcg gttcacgttt acgtagattg
    10921 cgtcctcggg gaccgccagg ttctgccgtg ccgactcgat gatcctgatg ttcgcctggt
    10981 ggggcaccag caggctgacc tcttcggggc ggatgcccgc tttctccagc gtctgatgga
    11041 aggctcgggg gaggatgcgg gaggcgaact tgaacacctc cttgccgttc atcttaacgt
    11101 agtgctgtcg ccgttccacc gtatcgtggg tggcaggcgc cttggttcca ccggcgggga
    11161 tgatcaagtg ctcggcgccg gcgccgtcgg agcccaaaac gaaggagagg agtcctccct
    11221 ctttctcggt gggacgcaat actactgccc ctgcaccgtc gccgaagagg acacaggtgg
    11281 tcctgtcggt ccagtccagc accctggtca tcgactctgc tccgatcacc agcacgttcc
    11341 ggcaggcgcc gttctggatg aactgtgagc cggtgaccag tccgcagacg aagccggagc
    11401 agcccgcgac caggtcgaag gcaccgcagc acttgatgcc gaggatattt tgcaccagcg
    11461 aggcgctggc cgggacgttt gggtaatcac ccgtgatggt ggccaggatg atgaggtcga
    11521 tgtcctgggc ccctaccccc gccctctcca tggcctgcct ggcggcggcc gcggcgatgg
    11581 tcgaggtggt ttcatcttcg gagatgatcc ggcgctcgga aatgccgctt cgactgacta
    11641 tccactcgtc ggaggtgtct actatcttct cg
//
LOCUS       DATALS010000493         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_355621, whole genome shotgun sequence.
ACCESSION   DATALS010000493 DATALS010000000
VERSION     DATALS010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355621"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VHS06_11005"
     CDS             complement(<1..1020)
                     /locus_tag="VHS06_11005"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX2988683.1"
                     /translation="MPKTRPVLNEPDGASKGSSANRPAPTSPHGRRFSQEELSRHDRE
                     VRATRMIFMGMGAFIVLVIAILGYGWWHEYVAASSEPVASVSGKNISIESYAKQLDYQ
                     RKRLEQQLSSMQFQLQQMGNDETMQSLTDMYKQQIQQVQFTLMLLPDQTLEQMISEQL
                     IRQEAAKRGISVSAQEIDSELATAFGDPPTPAPTTDSAAGQQAPASDATPAPTAPAAA
                     TQSAATPAPTAASAGAAASTPAPTAAADATPAPTADIQARVTDFATQYGLSRDDVRSL
                     VETQLLYQKLQDAMGNEVSTTAEQVHARHILVDSEDKAKEIVDKLKGGASFEDLAKSD
                     STDTGT"
     gene            complement(1133..1450)
                     /gene="rpsR"
                     /locus_tag="VHS06_11010"
     CDS             complement(1133..1450)
                     /gene="rpsR"
                     /locus_tag="VHS06_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX2988684.1"
                     /translation="MAFAPRPAGRPGGGRPGGGGGRPRYTPRRKVCAFCVDKIDHIDY
                     KDVARLRRYLSDRGKMEPRRKTGTCARHQRRLTIAIKRARHVALLPFTAEHIRQSGLT
                     IRG"
     gene            complement(1520..1921)
                     /gene="ssb"
                     /locus_tag="VHS06_11015"
     CDS             complement(1520..1921)
                     /gene="ssb"
                     /locus_tag="VHS06_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX2988685.1"
                     /translation="MAGLCKVTIIGNLGADPETRYTPEGKPKTSFRVACNRVYTVEGE
                     RREETEWFRVTAAGRLGEVASQYLRKGSKVYVEGRLRTGSWVGQDGEKRFTVDVFASD
                     LQMLDSKPRGGDMGDLPADEHSGVAEDQIPF"
BASE COUNT          348 a          642 c          638 g          451 t
ORIGIN      
        1 ggtgccggtg tcggtggagt cggacttggc gagatcctcg aagcttgccc cgcccttcaa
       61 cttgtcgacg atctccttgg ccttgtcctc gctgtccacg aggatgtggc gggcgtgaac
      121 ctgctcggcg gtggtcgaaa cttcattccc catcgcatcc tgcagcttct ggtagagcag
      181 ttgtgtctcc actagagagc ggacatcatc cctcgacagg ccgtactgcg tggcaaagtc
      241 ggtgaccctg gcctggatgt cggccgtcgg agcgggtgtc gcgtcggctg ctgcagttgg
      301 ggccggcgtc gatgcagcag cgcctgccga tgccgctgtc ggcgcaggtg tggccgcgga
      361 ttgggtcgcg gcggccggag cggtgggcgc gggcgtagca tctgaggctg gagcctgctg
      421 accggctgct gagtcggtgg ttggtgccgg cgtcggagga tcaccgaatg ccgtggctag
      481 ttcgctatcg atctcctgtg ccgacacgga gatgccgcgc ttggccgcct cctggcgaat
      541 caattgctcg ctgatcatct gttccagcgt ctggtcggga agcaacatca gggtgaactg
      601 cacctgctga atctgctgct tgtacatatc ggtcagcgac tgcatggtct cgtcgttgcc
      661 catttgctga agctggaact gcatggagga aagctgctgc tcgagcctct tccgctgata
      721 gtccagctgt ttggcgtagc tttcgatgga gatgttcttt cccgacacgg aggcaaccgg
      781 ctcggacgat gccgctacgt actcgtgcca ccagccgtag ccgagtatgg cgatgacgag
      841 tacgataaaa gcgcccatcc ccatgaagat catgcgtgta gcccgtacct ctcgatcgtg
      901 gcgggatagc tcctcctggg agaaacgccg cccgtgggga gaggtgggcg cgggccggtt
      961 ggcggaggaa cctttggatg ctccgtcggg ttcattcagt actgggcggg tctttggcat
     1021 gacttcctct gattgtcctg tagtgtgggt tcgtcccaat ggggcgctag agaaagaggg
     1081 catgagcccg aagaagactc acgcccccca atccaatcta gactaatgcc gactagcccc
     1141 tgatggtcag cccgctctgc ctgatatgct cagcggtaaa cggcagcagc gccacgtgcc
     1201 tggcacgctt gatggcgatg gtcaaccgcc gctgatgccg tgcgcaagtt ccagtcttgc
     1261 ggcgaggctc catcttgccg cgatcggaaa gatagcggcg cagccgcgcg acatccttgt
     1321 agtcgatgtg gtcaatcttg tccacgcaga aggcgcaaac ctttcggcga ggggtgtagc
     1381 gaggcctgcc gccaccgcct ccaggtcggc ctcctcctgg tcttccagca ggtctaggag
     1441 caaatgccaa cttcttttcc ctttctcgaa gcagccgatc accgcctata tgcggttcca
     1501 gccgttgtaa gggtccgagc tagaacggga tctgatcctc ggcaacgccg ctgtgctcgt
     1561 ccgcggggag atcgcccatg tcgcctccac gcggcttgct atcgagcatc tgcaggtccg
     1621 aggcgaagac atccaccgta aacctcttct cgccatcctg gccgacccaa ctgcctgttc
     1681 gcagtcgccc ctcgacgtac accttgctgc ctttgcggag gtactggctg gcaacctcgc
     1741 cgagcctacc agcggcagtc accctgaacc actcggtttc ctccctgcgc tcgccctcta
     1801 cggtgtagac gcggttacag gctactcgaa acgacgtctt gggcttgccc tccggcgtgt
     1861 agcgcgtctc gggatcggct ccgaggtttc cgataatcgt cactttgcac agtcccgcca
     1921 tccccgaacc tccctcttag tcctgacgaa ccaacatata gcggatgatc tggtcgtcca
     1981 gctgtttggc gtagctttcg atggagatgt tctttcccga cacggaggca accggctcgg
     2041 acgatgccgc tacgtactcg tgccaccagc cgtagccga
//
LOCUS       DATALS010000494         1748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_355674, whole genome shotgun sequence.
ACCESSION   DATALS010000494 DATALS010000000
VERSION     DATALS010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355674"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VHS06_11020"
     CDS             <1..267
                     /locus_tag="VHS06_11020"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483493.1"
                     /note="catalyzes the formation of
                     2-N(omega)-(L-arginino)succinate from L-citrulline and
                     L-aspartate in arginine biosynthesis, AMP-forming; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX2988686.1"
                     /translation="LAYAGLWYDPFKDDLEAFINKIQPRVTGTVTMRLFKGSAQMIGR
                     SSKYALYNEDLASFDSKTFDQSEAVGMVKVHGMQSRMFNTVKDA"
     gene            326..514
                     /locus_tag="VHS06_11025"
     CDS             326..514
                     /locus_tag="VHS06_11025"
                     /inference="COORDINATES: protein motif:HMM:NF040165.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988687.1"
                     /translation="MIDDTVAAILSTKTPAEKVEMVLEANRFARLLLRTTLHSRYPAW
                     TQEQVDREVSRRMLSGAD"
     gene            501..1058
                     /locus_tag="VHS06_11030"
     CDS             501..1058
                     /locus_tag="VHS06_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988688.1"
                     /translation="MEQTEILRYVVEVLEKLQVPYMLVGSISSAVYGEPRMTLDIDVV
                     VQLVPDEIEHLCEAFPPEEYYVSQEAALDALAHEGQFNVIHASSGNKVDFMVARSDAW
                     GVEQLCRRVRLELLPGLHGYVASPEDVIIGKMLYYRDGGSEKHLRDIVGILKTGPQEV
                     DKDYVRKWAGVLGLSDIWEITVARL"
     gene            1206..1667
                     /locus_tag="VHS06_11035"
     CDS             1206..1667
                     /locus_tag="VHS06_11035"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HEX2988689.1"
                     /translation="MSDMQHFLTDDFRGLDPQSRKQRAAEARQISAIAAGAIAPMPIP
                     FADIWTITPIQILMVRAIANIYGYGLDVTTAKAVLATVGGGWLGRQTFLALLKLGLPG
                     FGEAASAGFAYFWTLGMGRAAEAYLASGMSASRRQMRRAMTELPITPIPPQ"
BASE COUNT          373 a          457 c          551 g          367 t
ORIGIN      
        1 cttgcttacg caggtctgtg gtatgacccg ttcaaggacg acctcgaggc gttcatcaac
       61 aagattcagc ctcgcgtcac gggcaccgtc acgatgcgcc tgttcaaggg cagtgcgcag
      121 atgatcggcc gatcgagcaa gtatgcgctc tacaacgagg accttgccag ctttgatagc
      181 aagacgttcg accagagcga ggctgtcgga atggtcaaag tccacggtat gcagtcgagg
      241 atgttcaata ccgtcaagga tgcctagcgc ctgcagttcc gagaggacaa tcgtgagaga
      301 gcggctggac ccaagacgta tctaagtgat cgacgacact gtggcggcaa tactttcgac
      361 caagactcct gcggagaagg tcgagatggt gctggaggca aaccgcttcg cacgcctgtt
      421 gctgagaacg acgctgcata gcagatatcc ggcttggacc caggagcaag tggatcgtga
      481 ggtctcacgg aggatgctta gtggagcaga ctgagatcct gcgttacgtg gtcgaggttc
      541 tggagaagct tcaggttccg tacatgctcg tgggatcgat ttccagtgcc gtttacggtg
      601 aacccagaat gacgctcgac attgacgtag ttgtgcagct cgtccctgat gagatcgagc
      661 atttatgtga ggcgttccca cctgaggagt actacgtcag tcaggaagcc gccctggatg
      721 cgttggcaca tgaagggcag ttcaacgtta tccacgcaag cagtggcaac aaggtcgact
      781 ttatggtcgc gcggtcagat gcgtggggtg tcgagcagtt gtgccgtcgg gtgaggctcg
      841 agttgctgcc tgggctccat ggatacgtgg cgagcccaga agacgtgata ataggcaaga
      901 tgctctacta tcgcgacggc ggctctgaaa agcatctgcg tgacattgtg ggcattctga
      961 agacaggccc gcaggaagtg gacaaggact acgtgcggaa atgggcgggc gttctggggt
     1021 tatctgatat ttgggagatc acagtagcgc gcctctagca tgccctgtgt ttcgccatct
     1081 acctctttgc ggaaccgtgg taatgggaga ccacggttcc gtctttgtac cacggtgacg
     1141 gaaccatccg gaggcacttc actatgtggc taccttggca tcttacgaag catctgcaga
     1201 agcacatgag cgatatgcag cattttctta ccgacgattt caggggtctc gatcctcaaa
     1261 gccggaagca gagggccgcg gaagcgcgac agattagcgc cattgcagcc ggcgccatcg
     1321 cgccgatgcc gatacccttt gccgatatat ggaccatcac gccaatccag attcttatgg
     1381 tgcgggccat cgccaatatc tacggctatg gactggatgt gactaccgcc aaggccgtct
     1441 tggcgacagt cggcggaggt tggctcggaa ggcagacatt cctcgcgctg ctgaagctcg
     1501 ggctgcccgg gttcggcgag gcggcgagcg cgggattcgc ttacttctgg acgctcggaa
     1561 tgggtcgcgc cgctgaggcg tatctcgcat ccggtatgag cgcgtcgcgg cgtcagatga
     1621 ggcgggcgat gacggaactg ccgataacgc cgatccctcc tcagtaggaa ctgcctgctt
     1681 gcctggtgaa gcacctactg atgagcgagg aggtgcctga gatgaacgtt cgcacggccg
     1741 tacccgcc
//
LOCUS       DATALS010000495         6020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_73953,
            whole genome shotgun sequence.
ACCESSION   DATALS010000495 DATALS010000000
VERSION     DATALS010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73953"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VHS06_11040"
     CDS             <1..918
                     /locus_tag="VHS06_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2988690.1"
                     /translation="EDVSMCVLPLSHLHGQMVSMVATICSGGTLILPKRFNAGRFWDI
                     LRQHHVTWFSAVPPILNVLHQSIVAPPPHTCTLRFARSSSAPLSLAVLKEFEEIVGCP
                     LVEAYGLTEAGGHVATNPLPPRIRKPSSVGLPTGVQVAILGESRAPMPVGEEGELALM
                     GEGITRRYYRNSNANAQARVGTWFLTGDLGYVDSEGYIYITGRKREQITRGEERISPR
                     EVDEILHRHPKVAEAATVGVPSPTYGQEVEAYVVLRAGESASEIEIRNHCALHLTESK
                     CPKNIRFVPTLPRSPNGKIQRYRLLDAGS"
     gene            complement(1378..1959)
                     /locus_tag="VHS06_11045"
     CDS             complement(1378..1959)
                     /locus_tag="VHS06_11045"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2988691.1"
                     /translation="MKGFVVGRAVLVAILGAMLLLASGCLGGSRNFSPGDGSAAQALT
                     AQSADVRPDVGPRVGSQTPDFALKGLNGQTVRLSELRGKPVMVNFWATWCPPCRQEMP
                     DIEKAYKKYRDEGIVFLGVDQMEDPDIVQKFVKENGYSWVFLLDSEGEASKQFRASAL
                     PTTFFIDRQGVIRDMQIGALTAAQLESKLSKIR"
     gene            complement(2101..2811)
                     /locus_tag="VHS06_11050"
     CDS             complement(2101..2811)
                     /locus_tag="VHS06_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX2988692.1"
                     /translation="MDPQNLSLLVAFGAGLLSFLSPCVLPMVPIYVAYLAGCSANAVT
                     DGRRRIALANSLAFGAGFTMVFVGFWASFGLVGYVLSDYSDLLRQVGGAILIMMGLHQ
                     AGLLRIPLLYRQLRRDLQLTGSATPATSVAMGLAFAAGWTPCVGPVLAGIIGLASMSN
                     TVLEGVALLVAYSLGLGVPFLATALLLDRATALMDRMKRHMRIVSISTGVLLVAIGLL
                     MLSNTFRLLPQYFNWGAV"
     gene            3026..3520
                     /locus_tag="VHS06_11055"
     CDS             3026..3520
                     /locus_tag="VHS06_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2988693.1"
                     /translation="MVDTETTSEQVAVLELDVLPRLMRLVATSAGLREPGQLLSTTQV
                     RILKRLVVRPWLGSELAHELRVTPPTVSAAIDSLVRRGLVERGEAEDDRRAVPLRLTP
                     SGARCFERTKERALATLQSIADQIGPEERHGLALGLSAIAGILDSQSAPHNCNQSLTI
                     EDEG"
     gene            3648..4325
                     /locus_tag="VHS06_11060"
     CDS             3648..4325
                     /locus_tag="VHS06_11060"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX2988694.1"
                     /translation="MVVIPVLLIVLAVASAVGYRYWWNDVHYVSTDNAQIAGALVQVG
                     AMNAGRVDSVSVDVGDSVQEGQVVGTVLLPSTISVSQSGAPRLDFVGSENQRADIKAS
                     ISGIVVARQANVGDTVNTGQSIVTLVDPNRLWVQAQIEETKIGRVRVGLPVEVSVDSL
                     GKTLNGKVVAINRASSATFSLLPSGNSSGNFTKVTQLVPVKISVDYGDHPLVLGSSVE
                     VHIRVQE"
     gene            4330..5919
                     /locus_tag="VHS06_11065"
     CDS             4330..5919
                     /locus_tag="VHS06_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX2988695.1"
                     /translation="MSNVPVPKPHRRLRALPYYWQAVIVTIIGSFMVMLDTTVVNIAL
                     PRIITVFGADVHSSQFVLTFYMLALAIIMPATGYLTDTYGTKRIYLLSMLFFTIGSFL
                     CSAAWSVPSLVVFRVLQGLGGGMLMPLSMTIIFKTVPPEKRGTVMGVFGLPMLLGPVL
                     GPTLGGYLVEYVDWRFIFTLNLPVGALGLLLGATLLRETETRAGLKTDVKGFLFSGTG
                     FAALLYALSEAPAEGWDDPFIVSLLTLGGVCLLAWLWTELTVDEPLVDLRVFRSGTYS
                     LATFINFIITTGLFSSMFLLPIFLQNFRGLGAMETGILMMPQALASTITMPLSGKLYD
                     RFGPRPVVAVGLLGLAFSTWELTFLNLDTSNADVTRVLVERGLSMGLTMMPIMTVAMS
                     SIPPELIARSSSLTNVLRQVFGSFGTAIFATLLSTRQTFHQALLTQMVTPDRLSVQAT
                     LASVQQYLSHNGLSVVQAKGLTVAALYKQVVMSASVTAFQDCFFVAALICLVAVIPTL
                     LLRVKPGLAGGRQHPRQMATD"
BASE COUNT         1229 a         1872 c         1805 g         1114 t
ORIGIN      
        1 gaggatgtga gcatgtgcgt cctccctctg tctcacctcc acgggcagat ggtgtccatg
       61 gtcgctacaa tctgctcagg gggcacactg atacttccca agcgtttcaa cgccggtcgc
      121 ttctgggaca tactgaggca gcaccacgtg acctggttct cggcagtccc tccgatcctc
      181 aacgtactgc accaaagtat cgtggctccg cctccccaca cctgcaccct ccgtttcgcc
      241 aggtcctcct ccgcgcctct ctcgctcgca gtgctcaaag agttcgagga aatcgtggga
      301 tgtcctctgg tggaggccta tgggctcacc gaggccggcg gccacgtagc caccaatcca
      361 ctgcccccaa ggatccgaaa accatcctca gtgggtctac ccacgggtgt ccaggtggcc
      421 attctgggag aaagcagggc accgatgccg gtcggcgagg agggcgaact cgcgctgatg
      481 ggggagggca ttacccgaag gtattaccgg aactcgaacg ccaacgccca ggctcgtgtt
      541 gggacctggt tcctcacagg cgacctggga tatgtggact ccgagggcta catctacatc
      601 accggccgca agagagagca gatcactcgg ggtgaagaga ggatctctcc ccgagaggtg
      661 gatgagatac tccatcgcca ccccaaggta gcagaggccg ctaccgtcgg ggtccccagc
      721 ccgacgtatg gacaggaggt ggaggcatat gtggtgctcc gcgctggaga gagcgcgtca
      781 gagattgaga tacgaaacca ctgtgcgctt caccttacgg aatccaagtg tcccaagaac
      841 attcgcttcg tgcccacgct acccaggtcg ccaaacggaa agatccagag ataccggcta
      901 ttggatgcag gatcctaggc gacccacagg atgcagcact cacacgcccc aaactgtggc
      961 acccggactg tcaacccgat agcttcaccc agcttctgtc tggatgaggc gaacatcagg
     1021 gaccgctggg tccactctac tggattggct gcaggctcct cgggggtcat cgcccgcggc
     1081 tcgttcaggg cggcaaaact cgagagagca ccggagcctc gatgtcttgg ggggaatgga
     1141 ggtggggagt atcggatact agagcaactc catggtctgg taccgacact ctgaccgagg
     1201 aggttgccga tgaagaaacc gttgggccgc ctcgcctgat ctgcctggtc ggcagagaga
     1261 acgaaaggcg gggtcatccc acgggatctc aggacgacta acatccaaca catgctctgg
     1321 ttggggatgg ggcgaagggt cccgtcccca accggcaggg tggggcaaga agaatcctca
     1381 ccgaatcttg gacagcttgg actccagttg ggcggcagta agagccccta tctgcatatc
     1441 gcggatgaca ccctgccgat cgatgaagaa ggtggtgggt agtgcactgg cgcggaactg
     1501 cttgctggcc tcgccttccg agtcgaggag gaagacccag ctatagccgt tctccttcac
     1561 gaacttctgc acgatgtccg ggtcctccat ctgatccacc ccaaggaaga cgataccctc
     1621 atcgcgatac ttcttgtagg ctttctcgat gtcgggcatc tcctgtctgc agggggggca
     1681 ccacgtcgcc cagaagttca ccatcaccgg cttgccccgc aactcggata gtctcaccgt
     1741 ctggccgttc aatcccttga gagcaaagtc cggagtctga ctgcccaccc gcggccctac
     1801 gtccggccgc acgtcggcag attgagcggt caatgcctgg gcagccgagc catcaccggg
     1861 cgagaaattg cggctgcctc caaggcaacc cgacgcgagc aacagcatcg cgccgaggat
     1921 ggcgaccaac acagcccgcc ccaccacaaa gcccttcata tcgtcttgca ccccaagtca
     1981 gctgtctgta cgggggtcag gggacgggga tcgggggcca gaagccctca cccgcccttg
     2041 agaccgcccc cttcgaagag caacatcgct cgtcaccggc ccctgacccc cgtcccccac
     2101 tcacacggct ccccagttga agtactgggg cagcagccgg aaggtgttgg acagcatcag
     2161 cagccctatg gccaccagaa gcacgccggt agagatggat acgatcctca tatgccgttt
     2221 catccgatcc atcagcgccg ttgccctatc caacaacagg gccgtcgcga ggaacggcac
     2281 acccaacccg agggagtagg ccacgagcaa tgccaccccc tccagcacgg tgttgctcat
     2341 gctggcaagg ccgattatcc ccgcgagaac aggcccgacg caaggggtcc aacccgccgc
     2401 gaaggcaagc cccatagcga cagaggtggc tggagtcgcg ctcccggtca gttgaagatc
     2461 gcggcgcaac tgtcggtaga gcagcggaat cctcaacaac cccgcctggt gaagccccat
     2521 catgatcaag atggcgccgc ccacctgacg caaaaggtcg gagtaatccg agagtacgta
     2581 gcctaccaga ccgaaggaag cccagaagcc tacgaagacc atggtgaaac ccgcgccgaa
     2641 ggcgagcgag ttggccaatg cgatccgcct gcgaccgtcg gtgacggcgt tggcagagca
     2701 gccggccaga taggctacgt agatgggaac catgggcagc acacagggcg aaaggaagga
     2761 aaggagtccg gcgccaaagg cgaccagaag tgaaaggttc tgcggatcca cgcagtggca
     2821 ccccgatccc cagagaatca cggcacggca agggctcgtg tcgccggagc agtcatcatg
     2881 tcggtcgaat tcatcctaga tggcgctgcc cactatgtca accaaccgtc aaacaccgga
     2941 acgaaacgtg cttgtccacc ggaacgattt ctgctataac ctcccgcgat ttgattagtg
     3001 gtacttattt ttaggagtgt gcaccgtggt cgacacagag acaacctcgg agcaggtggc
     3061 cgtgctggag ctcgacgtcc tgcccaggtt gatgaggctg gtagcgacgt ccgcaggact
     3121 cagggagccg ggccagctac ttagcacaac ccaagttcgg atattgaaac ggctggtcgt
     3181 gcgcccctgg ttgggcagcg aactggcaca tgagttgaga gtcacccctc ccacagtgag
     3241 tgcggcaata gatagcctgg tgagacgcgg gctggtggaa cgtggagagg cagaggacga
     3301 ccgacgcgcc gtaccgctgc gactcacccc gtccggggcc cgatgcttcg agaggaccaa
     3361 ggagcgggct ctggccaccc tccagtccat tgccgaccag attggaccgg aagagaggca
     3421 cggccttgcg ctaggcctga gcgctattgc gggcattctg gattctcaat ccgcccccca
     3481 caactgcaat caatcgctga ctattgagga tgaaggctaa catgggagaa gttgttcagg
     3541 acagcaccac agccgtgcct ggcgccaggg cctctcaaga tggtgcatta catcatccgg
     3601 aagaagccgc gccccctcct agggcgaaga agcggggcgt gcccaagatg gtcgttatcc
     3661 ctgtcctgct gattgtcctt gccgtcgcga gtgccgtcgg ataccgttac tggtggaacg
     3721 acgtgcatta cgtctccacc gataacgccc agatagccgg tgcactggtc caggtgggcg
     3781 ccatgaacgc gggacgggta gacagcgttt ctgtagacgt gggcgacagt gtccaggagg
     3841 ggcaggttgt gggcacggtg ttgctcccca gcaccatcag cgtcagccag agcggcgcgc
     3901 cgcgcctcga cttcgtcggc agcgagaacc agagggcgga tataaaagcc tccatctcgg
     3961 gcatagtcgt tgcgcggcag gcaaacgtgg gcgataccgt caacacgggg cagtcgatag
     4021 tcacgctggt ggaccccaac aggctgtggg ttcaggcgca gatcgaggaa accaagatcg
     4081 gccgggtccg ggtgggcctg cccgtcgaag tgagcgtgga ctctctgggc aagacgctga
     4141 acggcaaggt ggtcgcgatc aacagggcta gctccgccac cttttctctg ctcccatccg
     4201 gcaactcgtc gggcaacttc accaaagtga cccaactggt tccggtcaag atctccgtgg
     4261 actacggcga ccatcccctg gtgctcggca gctccgtaga ggtccacatc cgagtccagg
     4321 agtgagtcga tgtccaatgt acctgtaccc aagccccacc gccgactgcg cgcgctgcct
     4381 tactactggc aggcggtgat cgttacgatt atcggctcct tcatggtgat gctggatacc
     4441 acggtggtga atatcgcgct gccccgcatc atcacggtgt ttggcgccga cgtccactcg
     4501 tcgcaattcg tgctcacctt ctacatgctg gcactggcca tcatcatgcc ggccaccggg
     4561 tatctgaccg acacctacgg caccaagcgg atctacctgc tctccatgct gttcttcacc
     4621 ataggatcgt tcctctgctc cgccgcttgg agcgtgccca gcctggtggt gttccgcgta
     4681 ctccagggat tgggtggcgg aatgctgatg ccactcagca tgaccatcat cttcaagacc
     4741 gtgccaccgg agaaacgggg cacagtgatg ggcgtgttcg gactgccgat gctgcttggt
     4801 cccgtgctgg ggccgacgct gggtggatat ctggtggagt acgtcgactg gcgtttcata
     4861 ttcacactga acctgccggt aggagcgctg ggacttctgt tgggcgccac actgctcaga
     4921 gagaccgaga cacgcgcggg cctgaagacc gacgtgaagg gttttctctt ctcggggact
     4981 ggcttcgccg ccctgctgta cgccctctcc gaggccccgg ccgagggttg ggacgatcct
     5041 ttcatcgtct cgctgctgac cctgggcggg gtctgcctgc tggcctggct gtggaccgaa
     5101 ctgacggtgg acgaaccgct ggtagatctg cgggtcttcc gcagcggtac ctactcgctc
     5161 gctaccttca tcaatttcat catcactacc ggcctcttct catcgatgtt cctcttgccc
     5221 atcttcctcc agaacttccg agggctgggt gcaatggaaa ccggcattct gatgatgccc
     5281 caggcactgg catccaccat aaccatgccg ctctccggca agctgtacga ccgcttcgga
     5341 cctagaccgg ttgtggctgt agggttgctg ggactagctt tctcaacctg ggaactgacc
     5401 ttcctcaacc tggacacctc caacgccgac gtgaccaggg tgctggtcga gagaggacta
     5461 tccatggggc tgaccatgat gccgatcatg acggtggcaa tgagttccat ccccccggag
     5521 ctgattgccc gctcgtcgtc ccttaccaac gtgctgcgcc aggtgttcgg ctccttcggt
     5581 acggccatct ttgccaccct gctatccacc aggcagacgt tccaccaggc gctgctgaca
     5641 caaatggtga ctccggacag gctgtccgtc caagcgacac tggcatcggt tcagcaatac
     5701 ctgtcccaca atgggctaag cgtggtccag gccaaggggt tgaccgttgc ggccctctac
     5761 aagcaggtag tgatgagtgc ctcggtgacg gccttccagg actgcttctt cgtcgcggcc
     5821 ctgatctgtc tggtggctgt catcccgacg ctgctgctac gagtaaaacc ggggctggcc
     5881 ggtggaaggc aacaccccag gcagatggca acggactagc cttatatcga cgacgcatcg
     5941 accgatggta ggggcggacc tgcgtgtccg cccctaccac ctatcaggca ctggatggcg
     6001 aaggaaaggc gtgagactgg
//
LOCUS       DATALS010000496         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_356279, whole genome shotgun sequence.
ACCESSION   DATALS010000496 DATALS010000000
VERSION     DATALS010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356279"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VHS06_11070"
     CDS             <1..314
                     /locus_tag="VHS06_11070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988696.1"
                     /translation="QQATSCALVINELLQNAVEHGFEVKNEGTIRVQLQKDDDECRIE
                     VIDTGQGLPPGFDMEKDGSLGLQIVQTLVREDLNGRFEMVDDGGVHALVAFPSHLTPE
                     A"
     gene            459..1046
                     /locus_tag="VHS06_11075"
     CDS             459..1046
                     /locus_tag="VHS06_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2988697.1"
                     /translation="MAQTRVVIADDESIIRMDLREMLSSLGYLVVGETGDGISAVNLA
                     RELRPDLVIMDIKMPDLDGIQAAKILTEERIAPVLLLTAFSQQELIEGAKDAGVVGYI
                     VKPFRESELVPAIEVALARFREFKALEKELTDTRNILETRKIVERAKGVLMDTQGLKE
                     AEAFRKIQKLSMNTRKSMREVAEAILLANQVGKEG"
     gene            complement(1133..1291)
                     /locus_tag="VHS06_11080"
     CDS             complement(1133..1291)
                     /locus_tag="VHS06_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988698.1"
                     /translation="MEEQDLVGLTVSDGFKFGCGFSLALTIGFLAMLLVFTCFVVVGM
                     LLGVKLPG"
     gene            complement(1327..1875)
                     /locus_tag="VHS06_11085"
     CDS             complement(1327..1875)
                     /locus_tag="VHS06_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX2988699.1"
                     /translation="MDKERYDRSYYEEGDQGDESEVVAPVRRRRDPSEQAHARLLALM
                     DELETIIDKGARIPFSSKVLIDRDVYLDAVDALRMTMPEALTQAERIVRDKERIIAQA
                     SAESERITSLAKEQAAFLISERQLLRAAETQSEAILSSAREESKEIISSAQKYASDLL
                     AQLEADALRVLGEIRKAASQAR"
     gene            2775..>4383
                     /locus_tag="VHS06_11090"
     CDS             2775..>4383
                     /locus_tag="VHS06_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX2988700.1"
                     /translation="MSYGYRTAQSSVPGSSAPMTWVGSTLDFALSQIPAQKILLGVPG
                     YGYDWDTTSGPPAKAMRYSDAMAIANQQGIGVQYDTTQDSVRFGYTKDGHTHDVWFED
                     RSTFTTRVALVGQRGVAGFALWRLGHEDPQIWQSAIIQPPAPTATPAAATASPTPTQA
                     STSGQKSGTTLYFAEGSTASPFDSWFLLQNPAQSPASATVTFMRDDGSAESRRYDLPP
                     TSRLSIFANQIVPNSAFSTMVESSQTILAERAMYAGFDGHDVTAVTSPSRLWYFAEGA
                     TTQPFHSWLLLQNPNQAATTAKVRFLLENGSVVEQGQFLPPRSRVSLFVNQILPDAAF
                     STKVESEQPIIAERAMYRFPGNAATAVTGVAAPSRNWYFAGGMPSGRNQPFDSWLLLQ
                     NPGSAPVTASITLFTPDGQQTAFQQFLPPTSRQSLFLNQLTSASSYGIRVEATGEIIA
                     ERSIYLNATTASGNQLQGAHATQGAPEMGKVWALPEGSTASPFTERISVLNPHASPRS
                     VRFEFMLENGQTQIQNATIQPGCNYDLD"
BASE COUNT          897 a         1505 c         1204 g          777 t
ORIGIN      
        1 cccagcaggc tacgtcgtgc gctctggtaa tcaacgagct acttcagaac gccgtcgagc
       61 atggcttcga ggtgaagaac gagggcacca ttcgagtcca actgcagaaa gatgacgacg
      121 aatgccgcat cgaggtcatc gacaccggtc aggggctgcc ccccggtttc gacatggaga
      181 aggatgggag tctcggcctc caaatcgtcc agaccctggt gagagaggac ctgaatggtc
      241 gctttgagat ggtcgatgac ggtggcgttc acgccctggt cgcattcccg agccatctca
      301 ctcctgaggc gtaggtttca ccaggttgac gctgccttat gcctcagtat attatgactg
      361 caaatcattg acccgtagtc gttacgaaag aacgccaccg caatcggcgt tctcgagtct
      421 cgcttatccg aaggtcagtt caacaaggag agactgagtt ggcacagaca cgcgtggtca
      481 tcgccgacga cgagtctatc atccggatgg atctgcggga gatgctcagt agcctgggtt
      541 acctggttgt gggcgaaacc ggggacggca tcagcgcagt caacctggcc cgcgaactga
      601 gacccgacct ggtcatcatg gacatcaaga tgccagacct cgacggaata caggccgcca
      661 agatcctcac ggaggagcga atagccccgg tgctgctgct gaccgccttt tctcagcagg
      721 agctgatcga gggagccaag gatgcaggag tggtcggcta catcgtcaag ccgttccgtg
      781 aatccgagct ggtccctgca atagaggtgg ccttggcccg tttccgtgag ttcaaggcgc
      841 tggaaaagga actaaccgac accaggaaca tcctggaaac gcgtaagatc gtcgagcgag
      901 ccaagggtgt cttgatggat acccaggggc taaaggaggc cgaggctttc aggaagatcc
      961 agaagctgag catgaacact cgaaagtcca tgcgcgaggt cgccgaagcc atcctgctgg
     1021 ccaaccaggt cggcaaagag ggctgatccc ccaacacccg ataggctctg cataacgtca
     1081 aggcccgccc caaatcatgg aggcgggcct ttgtcctaca tgctaccggg cgctatcctg
     1141 gcagcttcac tcccagcaac atacccacca cgacgaagca ggtgaacacc agcaacatcg
     1201 ccagaaagcc gatggtgagg gcgagagaga aaccacagcc aaacttgaac ccgtcgctaa
     1261 ccgtcaatcc caccaagtct tgctcttcca cgcctctcga aagcacgctc cactagttta
     1321 cttcgtctac cgcgcctggc tggccgcctt ccggatctca cccaacacac gaagcgcatc
     1381 agcctccaac tgggccagca ggtcgctcgc gtacttctgc gcagaggaga taatctcctt
     1441 ggactcctct ctcgccgacg acaggatcgc ctcggattgc gtctctgccg cccgcaacag
     1501 ctgtctctca gagatgagga aagccgcctg ctccttagca agggaggtga tcctctccga
     1561 ctcggcgctc gcctgagcga tgatccgctc cttgtcccga acgatccgct ctgcctgcgt
     1621 caaagcctcc ggcatggtca tgcggagggc atcaacggca tcgagataga catctcgatc
     1681 tataagcacc ttcgagctga agggtatccg agcccccttg tctatgatcg tctccaactc
     1741 gtccatgagc gccaatagcc tggcgtgcgc ctgctcagac ggatcgcgcc gccgcctgac
     1801 cggcgcaacc acctcggatt cgtctccctg gtcaccctcc tcgtaatagg aacggtcgta
     1861 cctctcttta tccaaacctg cttcctccgt ggtgggtctg gggctcgcat caggaacggg
     1921 gcgctgtgaa gaaaaggaga aatgcagcca tcgcacccgg gctgccctgc cgcgcggcat
     1981 tatatcacag tctacaccgc acaattcacc ccaggagatc ctccccgccg tgccccgtcc
     2041 gcctcttgac catccagctc cgcgagatgc agcacgcgcc gtgctataat gcgctccatc
     2101 ctccacttct gtgagtcagt tcatgctgct ttctcgcttc cggctgattg ccctggccac
     2161 ccttctctct gccgccacct gcgcgcatgc cactatggcg acaacatcgc ccaccatcgc
     2221 ggaggcgcga accatcactg cgtcggccac actaaacagc ccgtccagcg cccaaacgcc
     2281 gatccgctgg gcctactacg tcccggagca atcctccctg gcgtccctcc aacagcgggc
     2341 cggccaactg gactacgttg cgctccactg ggccagtttc aaggaagatg gcacgctgga
     2401 ggtcaagacc aacccagacg tgctttccct ggttcgcagc ctcggcgcca agcctgtcct
     2461 ttccgtgaca gcgggcgtcg gaccagatat cgcccacgcc ctgctcgcga ccgatacctc
     2521 tcgggccgct gccgtggagg ctatcttcca gcaactcgcg gattacgacg gcgtcagcat
     2581 cgacttcgag gggctcaagc cggaggatcg tgaccccctc acccagttca tggcgcaatt
     2641 ggccgccagg ctcaggcccg ccggtaaact ggtcactttc gccctttccg ccaagacgac
     2701 cgacacccgc accggctggg ccggtgctca cgactacgcc ggcctcgccc cccaacgccg
     2761 atctcttcat tctgatgagc tacggctacc ggaccgcgca gagcagcgtt cccggctcgt
     2821 cggcccccat gacctgggtg gggtccacgc tcgactttgc cctatcccag atccctgctc
     2881 agaagatcct gctgggggtg cccggctacg gctatgactg ggacaccacc tccggcccgc
     2941 cggccaaggc gatgcgctat tccgatgcca tggccatcgc aaaccaacag gggatagggg
     3001 tgcagtacga caccacgcag gactccgtcc gcttcggcta caccaaggac ggccacaccc
     3061 acgacgtctg gttcgaggac aggagcacct tcaccacgag agtagcgctg gtgggccaac
     3121 gaggcgtggc cggcttcgcc ctgtggcgac tagggcacga ggatccccag atatggcaga
     3181 gtgccatcat tcagcctcct gcgcctaccg caacgccggc cgctgcaaca gcttcgccga
     3241 cgccaaccca ggcatccacc tccggacaga agagcggcac aaccctctat ttcgccgagg
     3301 gcagcaccgc gtcgccgttc gatagctggt tcctgctcca gaaccccgcc cagagtcctg
     3361 cctcggcaac agtcaccttc atgcgcgacg acggatcggc ggagtctcgc cgctacgacc
     3421 taccgcccac ctctcgtctc agcatcttcg ccaaccagat cgtgcccaac tccgccttct
     3481 caaccatggt ggaatcgagc cagacgatct tggccgaaag ggcgatgtac gccggcttcg
     3541 acggccacga cgtcaccgcc gtcacatctc cgtcgcggct ctggtacttc gccgagggcg
     3601 ccaccacaca gcccttccac agctggctgc tactccagaa tccaaaccag gccgccacga
     3661 cggccaaggt gcgttttctg ctggagaatg ggtcggtggt ggagcaaggc cagttcctgc
     3721 cccctcgctc tcgagtcagc ctcttcgtaa accagatact gccggatgcc gccttctcga
     3781 ccaaggtgga aagcgagcag ccaatcatcg ccgagcgagc gatgtaccgc ttcccaggga
     3841 acgcggcaac cgcggtgacc ggagtagccg ccccatcccg caactggtac ttcgccggtg
     3901 gaatgccctc cggtcgcaac cagcccttcg actcgtggct cctgctccag aaccccggtt
     3961 cggcgccagt gacagcctca atcaccctct tcaccccgga tgggcagcag acggcctttc
     4021 agcagttcct gccacccacc agccggcaga gcctgttcct gaaccagctc acttcggcgt
     4081 ccagctacgg gatacgtgtg gaggccactg gagagatcat cgcggagagg tccatatacc
     4141 tcaacgccac gactgcctcc ggtaaccagc tccagggagc ccacgcgacc cagggcgcgc
     4201 ccgagatggg taaggtgtgg gcattacccg agggctcgac ggccagcccc ttcacggagc
     4261 ggatcagcgt actgaacccc cacgcgagcc cgaggtcggt tcggttcgaa ttcatgctgg
     4321 aaaacgggca gacccagatc cagaacgcca ccatccagcc cggatgcaac tacgacctcg
     4381 acg
//
LOCUS       DATALS010000497         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_74310,
            whole genome shotgun sequence.
ACCESSION   DATALS010000497 DATALS010000000
VERSION     DATALS010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74310"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VHS06_11095"
     CDS             complement(<1..541)
                     /locus_tag="VHS06_11095"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX2988701.1"
                     /translation="MLAIFAIMLVLFVVAMPVAWSMAAASAAYMAFATGVPLQGMVQR
                     MIGGIDTFPLLAIPYFILAGNLMNTGGITDRLVNFAKAMVGHITGGLAHAVVVTKMIM
                     AGMSGSGVADAAGSGTILIPAMKKAGYSVPFSAAIVAASGTIGPIIPPSIPMVLYGSI
                     ASVSIGRLLLGGAVPGVVMG"
     gene            complement(541..1020)
                     /locus_tag="VHS06_11100"
     CDS             complement(541..1020)
                     /locus_tag="VHS06_11100"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX2988702.1"
                     /translation="MGPLRFLVRNLEEILSGIFLVLMFLSTFTNVIARYFFNHPFPWA
                     EEFSRYAFIWLSFLGAALCTKHGRHIVIDGFVRAVPFRAEKAINILVSIMMIVLAYVM
                     IYYGWILCTNATQPTATLKVPQYFVYISVPFSGFLIFLYTLRDIRRDFRAVTNGGER"
     gene            complement(1102..2277)
                     /locus_tag="VHS06_11105"
     CDS             complement(1102..2277)
                     /locus_tag="VHS06_11105"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX2988703.1"
                     /translation="MRTFSKRFLGAISLVLIAAVAAMTLAACSSSAPAAAPTKAPEPA
                     KAAEPTKPAAPAATTAAAPAATTAPAAAPAAAAVSFPKMDVKLGHGGVLDMSYHKGNV
                     KFSELMKERSGGNVNVQVFPNSQLGSEKDMLEQVKNGVIQISLTTPVMLANYEGWGQI
                     GVTAMPYIFKGDTDEDQYPALMKMMRGPLFKEVNDKAAKISGIRGLDLGWWYGTRNLT
                     NKVREIKTADDIKGLKVRTMDAPLARGALDALGAATTPMAVAELYSAMQMGVVDGQEN
                     PYNTIYASKYHEVQKYLAATGHMTMTVLMVCNDKWFQSFTPEQQAVIVKSMQDAGDYQ
                     SDIQLKMNNQNLKDLQTAGLVFSKPDKAQLAEKTKDVYKQFGNMFTADFYTQVKNGQ"
     gene            complement(2592..>2912)
                     /locus_tag="VHS06_11110"
     CDS             complement(2592..>2912)
                     /locus_tag="VHS06_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX2988704.1"
                     /translation="IRVRGWAHDDEEASEGLQCVASPINNARGECVAAISIAAPSQRM
                     SEWRLEQLGSVVVQASLEISGLLGYRPAAAVQQDSSVPRPYRLHPIRDNGMTGKSDHP
                     PPDS"
BASE COUNT          527 a          843 c          944 g          598 t
ORIGIN      
        1 tgcccatcac cacgccgggg acggcacctc ccagcagcag ccgcccaatg gagacggagg
       61 ctatggagcc gtacagcacc atcgggatgc tcggcgggat gatggggccg atggtacctg
      121 aggcggccac gatggccgcg gagaagggga cgctgtatcc ggctttcttc atagccggga
      181 tcaggatggt tccggagccg gcggcgtcgg cgacgccgga accggacatc ccggccatta
      241 tcattttggt caccacgacg gcgtgggcaa ggccaccggt gatgtgcccg accatcgcct
      301 tggcgaagtt gaccagccga tcggtgatcc caccggtgtt catcaggttg ccggccagga
      361 tgaagtaggg gattgccagc aacgggaagg tgtcgatccc gccgatcatc cgctgcacca
      421 tcccctggag cggtactccg gtagcgaacg ccatataagc ggcgctggcc gccgccatgg
      481 accatgcgac gggcatggcg acgacgaaga gaaccagcat gattgcgaag atggcaagca
      541 tcagcgctca cccccgttcg tcacggcccg gaagtcccgc cttatgtccc tcagtgtgta
      601 caggaagatc aggaacccgg agaagggcac ggagatgtac acgaagtact gagggacctt
      661 gagggtggcg gtcggttggg tggcgttggt gcagaggatc cacccgtagt agatcatgac
      721 gtaggccagg acgatcatca tgatgctgac caggatgttg atcgccttct cagcgcggaa
      781 cgggacggct ctcacgaaac cgtcgatgac aatgtgccgc ccgtgcttgg tgcacagcgc
      841 tgcaccgagg aaggagagcc atatgaaggc gtagcgggag aactcttcag cccacgggaa
      901 cgggtggttg aagaagtatc gggcgatgac gttggtaaac gtcgacagga acatcagcac
      961 caggaagatg cctgacagga tctcttccag gttgcgaacc aggaagcgca ggggccccat
     1021 gggcgtcacc tccggcagat tgggggtggg actcggccgg atcccacccc tctggaagcg
     1081 gactaccaag ctaaaatcgg actactggcc gttcttgacc tgggtgtaga agtcggcggt
     1141 gaacatgttg ccgaactgct tgtagacgtc cttcgtcttc tccgcaagct gagccttgtc
     1201 cggcttgctg aagaccaggc ccgcagtctg gagatccttc aggttctgat tgttcatctt
     1261 cagctggatg tcgctctggt agtcgccggc atcctgcatg gacttgacga tgacagcctg
     1321 ctgctcgggg gtgaagctct ggaaccactt gtcgttgcag accatcagca cggtcatggt
     1381 catgtggccg gtggcggcca ggtacttctg gacttcatgg tacttggagg cgtagatggt
     1441 gttgtagggg ttctcctgcc cgtctacgac gcccatctgc atcgcggagt acagctcggc
     1501 cacggccatg ggggtcgtcg cggcaccgag ggcatccagg gcgccacggg cgagcggggc
     1561 atccatggtg cggaccttga gacccttgat gtcgtcggcg gtcttgatct ctctgacctt
     1621 gttggtgagg ttacgggtgc cgtaccacca accgaggtcc aggccacgga tgccgctgat
     1681 cttagcggcc ttgtcgttga cttccttgaa cagggggccg cgcatcatct tcatgagagc
     1741 gggatactga tcctcgtcgg tgtcgccctt gaagatgtag ggcatggcgg tgacgccgat
     1801 ctggccccat ccctcatagt tggccagcat caccggcgtg gtcagggaga tctggatgac
     1861 gccgttcttc acctgctcca gcatgtcctt ctcagagccc agctggctgt ttgggaagac
     1921 ctgcacgttg acgttgccgc cggagcgctc cttcatcagc tcggagaact tgacgttgcc
     1981 tttgtggtag gacatgtcca ggacgccgcc gtggcccagc ttgacgtcca tcttggggaa
     2041 ggaaacggcc gcagccgctg gtgcggccgc gggagcagtt gtggccgcag gggctgcggc
     2101 ggtagttgcg gccggagccg cgggcttcgt cggttcggct gccttggcgg gttcaggtgc
     2161 cttcgtgggt gcggcagcgg gggcagaact gctgcacgcc gcgagcgtca ttgccgccac
     2221 cgcggcgatg agtaccagac tgatggctcc aaggaatcgc tttgagaagg ttctcactct
     2281 tcctctccct ggttacattt cgttggcagc ccctatgggc tgccggccat tctggatggg
     2341 tacgaaataa acacgccatg ggaagcctgt tccgtctgta agattgttct acataggcgt
     2401 ctctccctct ggtcttccgc cctcagttga accgatattc gcgctgcccg gtggggccgt
     2461 attcctcccc ttcggcgtcg attgggacta aggacttgag caccattgcc cttcgagctg
     2521 tacgtctctg cgctgaggtc gggttggccg cgttctggac atcacacaat agtgcttgga
     2581 tcgagatcta ttcaggaatc gggaggtgga tggtccgact tgccggtcat gccgttgtct
     2641 ctgatcggat gcagtctgta tggccggggt accgacgagt cttgctgcac agccgcggca
     2701 ggtcggtatc ccagcaagcc cgagatttcc agactagcct gcacaacgac actgccgagt
     2761 tgctcgagtc tccattccga catccgctga gagggcgctg caatactgat ggccgcgacg
     2821 cactccccgc gtgcgttgtt gatgggactg gccacgcact ggagcccctc ggacgcctct
     2881 tcatcgtcat gggcccagcc tcgtacccgg at
//
LOCUS       DATALS010000498         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_356422, whole genome shotgun sequence.
ACCESSION   DATALS010000498 DATALS010000000
VERSION     DATALS010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356422"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VHS06_11115"
     CDS             <1..208
                     /locus_tag="VHS06_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988705.1"
                     /translation="NVRPNCSGLGASMLNHLPIESVIDSDTRPVASLPPLPGDRFEVV
                     PARVSIIRSAPDTVNGQKQLRYTQ"
     gene            complement(282..794)
                     /locus_tag="VHS06_11120"
     CDS             complement(282..794)
                     /locus_tag="VHS06_11120"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX2988706.1"
                     /translation="MKLEDKIRDVQDFPIKGVLFKDITTLLKDPDAYHQSIDVMYQKF
                     RDSGIELVVGMESRGFIFAAPLAYQLGAGLVPVRKLGKLPAETVRCEYALEYGTNTLE
                     IHKDAIKPGQKVLVVDDLLATGGTTIATLKLVEMLGGKVAGVAFLIELTFLHGRDKLK
                     GYDIFTQIQY"
     gene            complement(880..>2208)
                     /locus_tag="VHS06_11125"
     CDS             complement(880..>2208)
                     /locus_tag="VHS06_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX2988707.1"
                     /translation="KVDICIGTHRLVQKDVLFKDLGLVIVDEEQRFGVMHKERLKQLR
                     QQVDVLTLSATPIPRTLHMSLAGIRDMSTMETPPEDRLPIRTFVAEESDGMIREAILR
                     EMDRGGQVYFVHNRVHNIHFIASHLQKLVPQARIAVGHGQMPEDQLERVMLEFAEGKH
                     DILVCTTIIESGLDIPNVNTIVINQADRFGLAQLYQLRGRVGRGANRAYAYFLYNRNR
                     QLTETAEKRLKTIFENTELGSGFRIAMKDLEIRGAGNLLGVEQHGQVNAVGFDLYTRL
                     LADAVSELQGKAVRHKPEVTIDLPLAAHLPEEYVADEAARLSLYQRLASVKKEEELGG
                     LLEEMQDRFGALPLETQNLFYMLNLKLRAEQIGASSLSTLDGEIVLKLDGIPMATRRK
                     LQRRYGGRVRVLPNQIRLSRGEGDGWMPRLQEIVDSLEQEARELGGTQSS"
BASE COUNT          396 a          723 c          602 g          487 t
ORIGIN      
        1 caacgtgaga ccgaactgct cgggtttggg cgcttccatg ctgaatcacc tccccatcga
       61 gagcgtgatc gactcggata cgcgacctgt ggcatctctg ccacccttgc ccggagaccg
      121 tttcgaggtg gtgccggctc gtgtaagcat cataagatca gcgcccgaca ccgtcaacgg
      181 tcagaaacag cttcgctata cccaatagaa aaggggcggg ccgaataggc ccacccctgt
      241 catttctccc tggttcagcc ctgagatgga actgggagtt cctaatattg gatctgggtg
      301 aagatgtcgt aacccttgag tttgtccctg ccatgcagga aggttagctc gatcaggaag
      361 gcgacgcccg cgaccttgcc gcccagcatc tccaccaact tcagcgtggc gatggtggtg
      421 ccgccggtgg ccagcaggtc gtccacgacc agcaccttct gtccgggctt gatagcatct
      481 ttgtggattt ccagggtgtt ggtcccgtac tccagggcat actcgcagcg gaccgtctcg
      541 gccggcagtt ttcccagctt tcgaactgga accagccctg cgcccaactg ataggccagg
      601 ggggcggcga agatgaagcc cctggattcc attcccacca cgagctctat gccggaatcc
      661 ctgaacttct gatacatgac gtctatggat tggtggtagg cgtctggatc cttcagcagg
      721 gtcgtgatgt ctttgaaaag gacgcccttg atagggaaat cctggacgtc tcggatctta
      781 tcctcgagct tcattacgtc tcctctcttc cttgagccca tcgcatgacg gggaccggcg
      841 ggattctacc acaacgacgc ccaattccaa cgaggaactt caagaactct gcgttccccc
      901 aagttccctg gcctcttgct ccaggctatc cacgatctcc tgaagccgcg gcatccagcc
      961 gtctccttcc ccgcgactca gtctgatctg gttggggaga actcgaaccc tgcctccgta
     1021 gcgccgttgt agcttcctcc tggtggccat tgggatcccg tccaacttca gcacgatctc
     1081 tccatccagc gtggacagag agcttgcgcc gatttgttcg gcccgcagct tcaggttcag
     1141 catgtagaac aggttttgcg tctccagagg cagcgcccca aatcgatcct gcatctcctc
     1201 cagcagtccg ccaagttcct cttccttctt caccgaggcg agccgctggt agaggctgag
     1261 cctggccgcc tcgtccgcca cgtactcctc cgggaggtgc gccgccaatg gcagatcgat
     1321 ggtcacctcc ggcttgtgcc gaaccgcctt cccctgtagc tctgagactg cgtcggccag
     1381 caagcgagtg tacaggtcga agcccaccgc gttcacctgc ccgtgctgct ccacacccag
     1441 caggttgccg gcacccctga tttccaggtc cttcattgct atcctgaagc cggaccccaa
     1501 ctcggtgttc tcgaatatgg tcttcaatcg cttctccgcg gtctccgtta gctgccggtt
     1561 cctgttgtac aggaagtagg cgtaggctcg gttggcccca cgtcccaccc tgccgcgcaa
     1621 ctgatacagc tgggcaagcc cgaagcgatc cgcctggttg atcactatgg tgttcacgtt
     1681 ggggatgtcc aggcccgact cgatgatggt ggtgcacacc aggatgtcgt gcttcccctc
     1741 ggcgaactcc agcatcaccc tctccaactg atcctcgggc atctggccgt gacccaccgc
     1801 gatccgcgcc tgcggaacca gcttctggag gtggctggcg atgaagtgga tgttgtgtac
     1861 ccggttgtgg acgaagtaga cctggccgcc acgatccatc tcccgaagga tcgcctctcg
     1921 gatcatgccg tccgattcct ctgcaacgaa ggtgcgaatc ggcagccgat cctccggcgg
     1981 cgtctccatg gtgctcatgt ccctgatccc tgccaacgac atgtggagcg tcctgggaat
     2041 gggcgtggcg ctgagcgtca gcacgtccac ctgctggcgg agctgcttca gccgctcctt
     2101 gtgcatcacc ccaaagcgct gctcctcgtc cacgatcacc agccccagat ccttgaacag
     2161 tacgtccttc tggaccagcc ggtgggtgcc tatgcagatg tccacctt
//
LOCUS       DATALS010000499         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_122567, whole genome shotgun sequence.
ACCESSION   DATALS010000499 DATALS010000000
VERSION     DATALS010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122567"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VHS06_11130"
     CDS             <1..418
                     /locus_tag="VHS06_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988708.1"
                     /translation="LGTIVHMRNHRGIAGRLITTRSHNDPVSEAYRILRTNVEFASAG
                     ESPLMLIITGPAGHEGKSLTSANLAISFAQSGKRTLLVDADLRRPTLHTLFGDGDLGF
                     LNVRLNGIQLLLHKLASLLHLSYVFGCRALCDQLAS"
     gene            complement(524..850)
                     /locus_tag="VHS06_11135"
     CDS             complement(524..850)
                     /locus_tag="VHS06_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX2988709.1"
                     /translation="MARTEIGIASVRSELDLGQYLKTVRKWWWLILLSTGIAAGVSYY
                     SVRALPPTYQTYITLMAGEDAANPNVSADQVSLSERLADAYAGMVRREPILDATVRSL
                     RLPTSW"
     gene            complement(959..>1502)
                     /locus_tag="VHS06_11140"
     CDS             complement(959..>1502)
                     /locus_tag="VHS06_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988710.1"
                     /translation="HLAAVAYPEGSDTMSLLHLEWDGQSWAMPEIIVNSPPFPEYPRM
                     AIGQGNRLHLVWFDGDRASIDRTPVGVRYSSAQLTTPAVAAVRPAPNANSAKPPTEAT
                     PVGAPVSPVVGSPHGSDSAQEGTGSGPPGWREWVSDPEQRQVYPLVAGIGAALVLLGM
                     MAVAKAGTARLSGRDPRREG"
BASE COUNT          270 a          481 c          439 g          312 t
ORIGIN      
        1 cctggggacc attgtccaca tgcggaatca tcgaggtatt gccggtaggc tgatcaccac
       61 caggtctcac aacgatccgg tctccgaggc ataccgcatt ctccggacga atgtagagtt
      121 cgcttctgcc ggcgaaagcc ctttgatgct gatcatcacg gggccggcag gtcatgaagg
      181 caagtcgctg acgagcgcta acctggccat tagcttcgcg cagtccggca aacgaaccct
      241 cctggtggac gcagacctgc gccgtcctac gctgcacacg cttttcggag atggagatct
      301 cggcttcctg aatgtccgcc tgaatggaat ccaactgttg ctgcacaaac tggcgtcgct
      361 gctgcatctg tcgtacgtat tcggctgtcg ggctctgtgc gatcagttgg cgtcctagtt
      421 cctccgctat cgccttggcc ctctgcggat cggtgtctac gaccctgatc tctatgaact
      481 gagtccctcc tacccggttc accagtatcc tgccctgcag ttcctaccat gaggtcggca
      541 atcgcaggga tctcacagtg gcgtcgagga tcggctcccg ccgcaccatc ccggcatagg
      601 cgtctgccag tcgctcgctg aggctcacct gatcggcgct gacgtttggg ttggctgcat
      661 cttcaccggc catcagggtg atataggtct ggtaggtggg gggcaaggct cgaactgagt
      721 agtagctgac cccggctgcg ataccggttg aaagcagtat cagccaccac cactttcgaa
      781 cggtcttcag gtactgccct aagtccagct cggaccgtac cgacgcaatt ccgatctcag
      841 ttcgcgccat agctgttgtt ctccttccac tccccccgcg gcccgccgac atcggctgaa
      901 actactggac aggggtccaa cgggagcgat cgatacttct ggatgccccg aggggctctc
      961 acccctcgcg ccgagggtct ctgccggaca atcgggctgt cccggccttc gctaccgcca
     1021 tcattccgag taggacgagc gccgcaccta tgccggcgac caggggatac acctgtcgct
     1081 gttcggggtc cgatacccac tctctccagc ctggagggcc gctgccagtg ccctcttgcg
     1141 ctgagtcgga accatgaggg ctacccacga ccggtgagac aggggctcca actggggtcg
     1201 cctcggttgg gggcttggca ctgttggcat tgggagcggg ccgaaccgcg gccacggcag
     1261 gcgtcgtaag ctgggccgaa ctgtaacgga cgccgacggg cgtccggtcg atcgatgccc
     1321 ggtcaccgtc aaaccagacc aagtgcagtc gattcccctg tcctatcgcc attctcgggt
     1381 attctggaaa cgggggcgag ttgacgatga tctccggcat tgcccagctc tgcccgtccc
     1441 attcaagatg aagaaggctc atggtatcgg aaccctccgg ataggcaact gccgccagat
     1501 ga
//
LOCUS       DATALS010000500        10488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_74428,
            whole genome shotgun sequence.
ACCESSION   DATALS010000500 DATALS010000000
VERSION     DATALS010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74428"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VHS06_11145"
     CDS             complement(<1..698)
                     /locus_tag="VHS06_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988711.1"
                     /translation="MKSYRSCLLLAASLLAAGAACAAPRLLLFDSADGITERALQALQ
                     VPHTLASPDQYGSARVCLFDYRVAIWGMDADQSPLNADPGPAQSFVRIGGVFLAMRSQ
                     GDIAWAPRPLKQDKAYAVGQILKPDHPLLTTPHAFDAALLYKVHGGSIYRAFYALGEQ
                     WTPLVATGAEQSWDKTTPASPGPHYGLVEMKWGAGHIILCQMIPDYGLINDDKGQPGA
                     SRQFLDKPGVAALA"
     gene            complement(791..1744)
                     /locus_tag="VHS06_11150"
     CDS             complement(791..1744)
                     /locus_tag="VHS06_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988712.1"
                     /translation="MSRASLILLAVLLVPACLVGCGGRPPDQPGVSTAGTGLTGKIIF
                     CSNRRGNEDIYVLTLSPVSTVRVINHPAKDRHPVWSPDGTKFAFVSDRSGPWQIWLAN
                     ADGTGVTRLTHSGKCDTDPAWSPDGSRLAFARWDPDPDVHTHFICTVNADGSGARRIT
                     ALEGYCYDPDWAPDGSRIAYTARNADGNVDIYTMNASDGSGRTRLTNDPAPDSQPKYS
                     PDGGRIVFTSGRGGAGSSRLSPHSLWIMQATGAGEVPVGAGMSDDSAVWSPDGQHLAF
                     NGYGSPGGFDLYSIRPNGTERTQLNSSAGYNQSPSWWQPAP"
     gene            complement(1853..2815)
                     /locus_tag="VHS06_11155"
     CDS             complement(1853..2815)
                     /locus_tag="VHS06_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988713.1"
                     /translation="MRDPSVASALKARPAWSLAPLLLLLLLPLLSGCGGGNQSAPQAD
                     LKGGGFIGKLVFTSNVPGTSNYNLYTLTLPLPGTRKPIVSGPAQELSPVWSPDGTRAA
                     FVSNRSGHMQIYVVNADGSHLTRLTTNTYEDNNPCWSPDGLKIAFNRTKGPSTFICTI
                     NRDGTGSKALTATTGLAAEPAWSPNGARIAYLNGATGYCDIYSMSAVDGSKQVRLTTA
                     DSYDSSPAWSRDGARIVFVTDRGDTGDNASLWLMNADGTNQRRLTSGYHDQKPSYSPD
                     GAYIIFARFITGGRKIMGIRPDRTGLVAFTPGVGVDDGDPSWVE"
     gene            complement(3071..3910)
                     /locus_tag="VHS06_11160"
     CDS             complement(3071..3910)
                     /locus_tag="VHS06_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988714.1"
                     /translation="MQTAQGPETLTGKLLYASSRTGDYDIYIQTLGLFATTRRVARST
                     AADSQPAWSRDASRIAFVSDRSGRDQIYVVNTAGTGLQRLTFTALFDREPTWSPDGTR
                     IAFSRGSEIERAIWTMAGNGTGLRRLTPARGYATHPSWSPDGTRIAYARTSPPGTNLD
                     VWSMSATDGSGLLRMTRNVADDANPQYSPDGQWIAFESKRDNAGGLTSIWRMKADGTG
                     EIRVTSNANDFLPAWSPDGRRLVFQRALGAGSQLVTVSTTGISPIQLTNSLGFNVYAN
                     WAN"
     gene            complement(4057..6660)
                     /locus_tag="VHS06_11165"
     CDS             complement(4057..6660)
                     /locus_tag="VHS06_11165"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEX2988715.1"
                     /translation="MRSLALFALAVLVSLPALAADFPVLRGDQQAVIVYEAVAKDETV
                     KAYQDLAAYLQKSTGKTFSVVSDSDFTPGSALPIYVGRCNPVKQALRHDLARLDRDGY
                     IVAVEPSRVMLVGPKPWSTYWAVCQFLEDYAGVRWLIPGPLGEDITHSASLTVPPCKR
                     VYTPAMLSRLWSGAHYGGDWNLRQRIYARYNFHHNLINIFDVSKYWATHPEYFPIHGT
                     ERYKPGGKDDHAWQPCMGIDATVQVAADAAREAFRQDPNLESFSFGMNDGQGWCECPK
                     CKAIDKPVGEWHGFSGDKSVLFYTWLNKVAANLERDYPDKMLGCLAYSSAILPPPGMK
                     LHRNIIPYFTSNRADYFDPKFRAQDQQLMRWWAQHANQMGIYDYAYGVGFAVPRIYNH
                     LFQDAVKFAAANKVRGFYAEVYPNWGLDGPKLYTMARIVWDPNVNVDAVTADWNERMF
                     HEAAAPMKQYFATAEDAWRRQKGSGAWAYRLAADPAQFLVFPPAVMDEMTRCLDEAAG
                     LAKDDLVKQRIQFFRKTWELTRLLAGNYWAGRQVQDLIERKASVEEVARAMREMADRM
                     ANLDVDTYFQKTFANDPIAYFPPLAGWFTPLKGGAATNAIRYFASAVANEAVTLASND
                     KTLSGAAIREHVNRRCSEIFGADGSEQYRATVARMRDMALKIGTAARVQEPLKVDGAL
                     DELVWQKADVLSNFIIWGGTGPAANETRVRLAHDGKDLYIGLECLQDTSKLVCQSPAR
                     DGSTWKDDSVEIFINKDMNTSTYAQLIVNARGAFFDQYGNEATQSYAERLAHNFNASW
                     AAQVQPDRWTAEVRVPLAELGVNPAQVPLVRMNFVRNVCSAPETAISAWFSSLRAHSD
                     PLSRGWILLEQ"
     gene            6805..7986
                     /locus_tag="VHS06_11170"
     CDS             6805..7986
                     /locus_tag="VHS06_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988716.1"
                     /translation="MRMRPPLALLLFLGLLGPLAAASAQVPAGQQPARVQVVDSGLTR
                     KPPAEQGFDGLQYTSQGFTLSNGVVRYGIDYHAYWDAEGKTAKPSPEGYLGMPLPTGC
                     NWYHGGFMSVSFNGLNLGNIAPAVLRVVEQGQRGLIELLWKTAEGQVRIRFLQEPEAD
                     YLAAELAVLPTSEIRSLDLSLRCYPSYFTGWNKRDGWRQIIGPMTTTEQGQTKALDPL
                     QDWWLLYQDRVFDYALNRDDSAGPCGLLYLPEQIKSVQVLPTSYPVTTNVTVKPTVRS
                     VRLAFWEFPKKTNAESLQRLRQGAAQVAEHLRQVDFSDSQVAAFDAAGERAALAAMIA
                     KTKEPEAWRQRLAPMLDQVAAAQQAYRSNDLSAEQTASAALAKYRDAVWDLKFEALLS
                     D"
     gene            complement(8988..>10488)
                     /locus_tag="VHS06_11175"
     CDS             complement(8988..>10488)
                     /locus_tag="VHS06_11175"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX2988717.1"
                     /translation="QSLVLKSILWAAKKEPQVQFNDLGVGGDTINRAELTGKQLTAHW
                     RGATNGMQAHVTVRDEDKTLFLKTAQEIPGKDLSVKLPVLPAGKYFADVIIRAGQNCV
                     NWGSVGFTVVSDLNITALSLDKPAAKANETVTATATLSASAPADMNLYLEVVDNLDRL
                     IVRKSVTVAAGSKEIKVPFQLADPLALSARVTAYLVNGSPGKQPLEQACVAKASRELF
                     VPLKRSRGNYAHAVWSADGQRNDFVRRIMLRQLRACDVDLQTNAPTNVPGQAWLQSNN
                     FDTIPYATRYSYEGSNTVREPCLTDPKFLGPHLESLEKIGRELGPMGPRAYTLGDECF
                     LARDGVDVCFSPTCIADFQQWLQGEYGNIAALNASWGTNYGGFGEAQPITLQDAKKAG
                     RPVQWVDHRRHMEFVYARMMQRAGEAIRKADPEAEVGFDGPFDTNSFSGNDWSRLMQA
                     FTMVNVYFHQPTQWEFVRLIVSPPTPRSLNCTWGSFFAAHRMDLSTSDW"
BASE COUNT         1759 a         3142 c         3701 g         1886 t
ORIGIN      
        1 aacgcaagag cggctacgcc cggcttgtcc aggaactggc gggacgcccc tggctggccc
       61 ttgtcatcgt tgatgaggcc gtagtcaggg atcatctggc agaggatgat gtggccggcg
      121 ccccacttca tctcgacgag accgtagtga ggaccgggcg aggcgggggt ggtcttgtcc
      181 caggactgct cggcgccggt ggcgacgagg ggcgtccatt gctcgccgag ggcgtagaag
      241 gcacggtaga tgctgccgcc gtggactttg tacaggagag cggcatcgaa ggcgtgtggt
      301 gtggtcagca gcgggtggtc gggcttgagg atctgcccca ccgcgtaggc cttgtcctgc
      361 ttgagcgggc gaggcgccca ggcaatgtcg ccctgtgagc gcatggccag gaacacgcca
      421 ccgatgcgga cgaagctttg ggccgggccg ggatcggcat tgagaggact ctgatccgcg
      481 tccatccccc agatggcgac ccggtagtcg aagaggcaga cgcgggcgct gccgtactgg
      541 tcgggggagg cgagcgtgtg tggcacctgc agggcctgca gggccctttc ggtgatgccg
      601 tcggcgctat cgaacagcag caggcgcggt gcggcgcaag cggcgccggc ggccagcagg
      661 gaggctgcga ggaggaggca tgatcggtag gacttcatgt ggagcacctc tgaggaacgg
      721 tccgaacggc gggaaaagga gcggactgga aagtccgcgg tccacacggc aagtccacgg
      781 cggagagatc ttacggcgct ggctgccacc aggagggaga ctggttgtag ccggcagagg
      841 agttgagttg ggtgcgctcg gtgccgttgg ggcggatgct gtagagatca aaaccgccgg
      901 gagacccgta gccgttgaag gcgaggtgct gaccgtccgg ggaccagacg gcactatcgt
      961 cactcatgcc tgcgccgacc ggtacttcac cggcaccggt cgcctgcatg atccagagac
     1021 tgtgcgggga caggcggctg ctaccggctc cgccgcgccc gctcgtgaag acgatgcgcc
     1081 cgccgtcggg cgagtatttc ggctggctgt cgggcgcagg gtcgttcgtc aaccgggttc
     1141 tcccggaacc gtccgaggcg ttcatcgtgt agatgtcgac gttgccgtcc gcgttgcgag
     1201 cggtatacgc gatcctgctg ccatcggggg cccagtccgg gtcgtagcag tagccttcga
     1261 gagcagtgat gcgcctggcg ccgctcccgt cagcgttcac cgtgcagatg aagtgggtat
     1321 gcacgtcagg atcgggatcc catcgggcga aggcgagccg ggagccgtcg ggcgaccagg
     1381 cgggatcggt gtcgcacttg ccgctgtggg tcaagcgggt gacgccggtg ccgtcggcat
     1441 tcgcgagcca gatctgccag ggccccgagc ggtcactaac gaacgcgaac ttcgttccat
     1501 ccggggacca gaccggatgc cggtccttcg ccgggtggtt gatcacacgg acagtgctca
     1561 cgggactgag cgtaagaaca tagatgtcct cgttgcctct gcggttgcta cagaagatga
     1621 tcttgccggt gagtccggtg ccggccgtag agacgccggg ctggtcgggg ggacggccgc
     1681 cgcagccgac aaggcaggcg gggacgagca ggactgcgag aaggatcagt gaagctctgg
     1741 acatggcgat aagcctccca cgcgaggtgt gccggggtcg ctggcagtgc tgccgccgcg
     1801 gccgcggcgg cagcacctga tgagcttcca tggagccagt ctggagcagg ggctattcga
     1861 cccaggacgg gtcgccgtcg tctactccga cgcctggcgt gaaggcgacg agaccggtgc
     1921 ggtcggggcg gatgcccata atcttccggc cgccggtgat gaaccgcgcg aagatgatgt
     1981 aggcgccgtc gggggagtaa ctgggctttt ggtcatggta gccgctggtg agccggcgct
     2041 ggttcgtgcc gtcggcgttc atcagccaga gcgacgcgtt gtcgcccgtg tcgccgcggt
     2101 cagtgacaaa gacgatcctg gcgccgtcgc gcgaccaggc cggggaactg tcgtagctgt
     2161 ccgcggtggt aaggcgtacc tgtttggagc cgtccacagc actcatgctg tagatgtcgc
     2221 agtagccggt agcgccgttg agataggcga tgcgggcgcc gttgggtgac caggcgggtt
     2281 cggctgccag accggtcgtg gcggtcagcg ccttggatcc tgtaccgtcg cggttgatcg
     2341 tgcagatgaa ggtgctcggg cccttggtgc ggttgaaggc gatcttcagc ccgtcgggcg
     2401 accagcacgg gttgttgtct tcgtaggtgt tggtggtcag ccgggtgagg tgactgccgt
     2461 cggcgttgac cacatagatc tgcatgtgac cggagcggtt acttacaaag gcagccctgg
     2521 tgccgtcggg tgaccagaca ggcgacagct cctgggcagg ccccgacacg attggcttac
     2581 gtgtgccggg caggggcaag gtgagcgtgt agaggttgta gttgctggtg ccgggcacat
     2641 tgctcgtgaa gacgagcttg ccgatgaagc ccccgccttt gaggtcagcc tgcggggccg
     2701 actgatttcc cccaccgcat ccgctcagta gcggcaggag cagcaggagc agcagggggg
     2761 caaggctcca ggctgggcgg gcctttagtg cggaagcaac tgacggatcg cgcatgtgac
     2821 tcgtacctct ttctctgctc gtgtggagcg gctgcatcag gcggcgacac catcggggtg
     2881 ccggtacggt tctgcggcag agcgcaacgc cggagggcac tgccggaaga ccggcagtgg
     2941 cacccgcacg tggccctttt tccaacgagg tcgggcggca gctatgcatg agcccgcact
     3001 gccggaagac cggcagtggc acccgcaggt ggcttgtttc tcagcaaggt cggagcagca
     3061 gctctcgaag tcagttcgcc cagttggcgt acacgttgaa gccgagggag ttcgtcaact
     3121 ggatcgggct gataccggtc gtgctgacgg taacgagttg cgagccggcg ccgagggcac
     3181 gctggaagac caggcgacgg ccgtcgggcg accaggccgg taagaagtcg ttggcgttgc
     3241 tggtgacgcg gatctcaccg gttccgtcgg ccttcatgcg ccagatggaa gtcaggccgc
     3301 cggcattgtc acgcttgctc tcgaaggcga tccactgacc gtccggcgag tactgcgggt
     3361 tggcgtcgtc ggcgacgttc ctcgtcattc tcagcaggcc gctgccgtcg gtggcgctca
     3421 tgctccagac gtcgaggttg gtgccgggcg ggctcgtgcg ggcgtaggcg attctggtgc
     3481 cgtccgggga ccaggagggg tgggtggcgt agccgcgggc gggggtaagg cggcgcaagc
     3541 cggtgccgtt gccggccatg gtccagatgg cccgctcgat ttcgctgccc cgcgagaagg
     3601 cgatgcgggt gccgtcaggg gaccaggtgg gctcccgatc gaagagcgcg gtgaaggtca
     3661 ggcgctgcag acccgtgccg gcagtgttga cgacgtagat ctggtcgcgg ccggagcggt
     3721 cgctgacgaa ggcgatgcgc gaggcatcgc gcgaccaggc gggctgactg tcggcggcgg
     3781 tgctgcgagc cacgcggcgg gttgtggcaa agagtcccag cgtttggatg tagatgtcat
     3841 agtcgccggt gcggctactg gcgtacagga gttttccggt cagggtctcg gggccctggg
     3901 cggtctgcat agcatgctcc cccccaccac agccagccag taaggccacg ccgaccatca
     3961 ggcagataca gcaacaccac agttgagttc tcatcccaag ccacctcttc ctcgatgtca
     4021 cgtctcgcgg ctctgtcacc gcgtggacgt gggccgtcac tgctccagta gtatccagcc
     4081 gcggctgagg ggatcagagt gcgcgcgcag gctggagaac caggcggaga tcgccgtttc
     4141 cggggcggag cagacattgc ggacgaagtt catgcgtacc aacggcactt gcgccgggtt
     4201 gaccccaagc tccgcgaggg ggacgcgcac ctcagcggtc cacctgtcgg gctggacctg
     4261 ggccgcccac gaggcgttga agttgtgggc gaggcgctca gcgtagctct gggtggcttc
     4321 attgccgtat tggtcgaaga aggcgccgcg ggcgttgacg atgagctggg cgtaggtgct
     4381 cgtgttcatg tccttgttga tgaagatctc taccgagtcg tccttccagg ttgagccgtc
     4441 cctggcgggg gactggcaga cgagcttgct ggtgtcctgg aggcactcga gcccgatgta
     4501 gaggtccttg ccgtcatggg cgagacggac acgcgtttcg ttggccgcgg ggccggtgcc
     4561 gccccagatg atgaagttgg agagtacgtc ggccttctgc cagacgagct catcgagcgc
     4621 gccgtcaacc ttcagcggct cctgcactcg cgccgccgtc ccgatcttca gcgccatgtc
     4681 gcgcatccgc gctaccgtgg cgcggtactg ctcagagccg tcggcgccga agatctcact
     4741 gcagcggcgg ttgacgtgct cccggatcgc ggcgccggag agggtcttgt cgttgctggc
     4801 gagggtgact gcctcgttgg cgacggcgga ggcgaagtag cggatggcat tggtggcggc
     4861 gccgcccttg aggggagtga accacccggc cagcggcggg aagtacgcga tggggtcgtt
     4921 cgcaaaagtc ttctggaaat atgtatcgac atccaggttg gccatgcggt cggccatctc
     4981 acgcatggct ctggcgactt cctcgacgct ggccttcctc tcgatcagat cctggacctg
     5041 acggccggcc cagtagttgc cggcgagcag gcgggtcaac tcccaggtct tgcggaagaa
     5101 ctgaatgcgc tgcttgacga ggtcatcttt cgccagaccg gcggcttcat ccaggcagcg
     5161 ggtcatttca tccatgaccg cggggggaaa gacgaggaat tgggcggggt cggcggcgag
     5221 acggtaggcc caggcgccgc tgcccttctg gcggcgccag gcatcctccg ccgtcgcaaa
     5281 gtactgcttc atcggcgcgg cagcctcgtg gaacatccgc tcgttccagt ctgcggtgac
     5341 ggcgtccacg ttcacgttcg ggtcccagac gatccgtgcc atggtataga gcttcggccc
     5401 gtcgaggccc cagttcgggt acacctccgc gtagaagccc cggaccttgt tggcggcggc
     5461 gaacttcacc gcgtcctgga agagatggtt gtagatgcgc gggacggcga agcccacacc
     5521 gtaggcgtag tcgtagatgc ccatctggtt ggcatgttgg gcccaccaac gcatcaactg
     5581 ctggtcctgc gcgcggaact tcgggtcgaa gtagtcggcg cggttggagg tgaagtacgg
     5641 aatgatgttg cggtgcagct tcatgcccgg cggcgggagg atggcgctgg agtacgcgag
     5701 gcagcccagc atcttgtctg gatagtcgcg ctccaggttg gccgccacct tgttgagcca
     5761 ggtgtagaag agcacggact tgtcgccgga gaagccgtgc cactcgccca cgggcttgtc
     5821 gatggccttg cacttcgggc actcgcacca gccctgcccg tcgttcatcc cgaaggagaa
     5881 ggactcgagg ttgggatcct ggcggaaggc ttcccgcgcg gcgtcggcgg ccacctggac
     5941 ggtggcgtca atgcccatgc agggctgcca ggcgtggtcg tccttgccgc cgggcttgta
     6001 gcgctcggtt ccgtggatcg ggaagtactc ggggtgggtg gcccagtact tgctcacgtc
     6061 aaatatgttg atgaggttgt ggtggaagtt gtagcgggcg tagatgcgct ggcgcaggtt
     6121 ccagtcgccg ccgtagtggg cgccgctcca gagacgggag agcatagcgg gcgtatagac
     6181 ccgcttgcac ggtggcacgg tgagactggc ggagtgggtg atgtcttccc ccagtggccc
     6241 ggggataagc cagcgtaccc cggcgtagtc ctcgaggaac tggcagaccg cccagtaggt
     6301 ggaccagggc ttggggccga cgagcatgac gcgggagggc tcaacggcaa cgatgtagcc
     6361 gtcgcggtcc agccgggcca ggtcatgtcg cagggcctgc ttcacgggat tgcagcggcc
     6421 cacgtagatg gggagggcgg agccgggggt gaagtcgctg tcggagacca cactgaaggt
     6481 cttgccggtg gacttctgaa gataggctgc cagatcctgg tacgccttga ccgtctcgtc
     6541 cttcgcgaca gcttcgtaca cgattacagc ttgctgatcg ccccgcagga cagggaaatc
     6601 ggcagcgaga gcgggcaatg ataccagtac ggcaagagcg aatagggcga ggctgcgcat
     6661 tgcgggggga cctccgggtg atacgagcga cggtggtgtc ttctccgcgc gttgcgttca
     6721 gtcctgccgc ccgccagtac gaggaactga catctgctgc ggcgaaacgc tcttcgccgt
     6781 gctcacctct gctggaggcc gatcatgcgg atgcgcccgc cactagcact cctgttgttc
     6841 ctggggctcc tcggcccgct cgcggccgct tctgcacagg tcccggcggg gcagcagcct
     6901 gcccgggtgc aggtcgttga cagcgggctg accaggaagc cgccggcgga gcagggattc
     6961 gacggcttgc agtatacctc gcagggcttc accctcagca acggtgtcgt acggtacggc
     7021 atcgactacc acgcgtactg ggatgcggag gggaagacag cgaagcccag ccctgagggc
     7081 tacctcggga tgccgctgcc gacgggctgc aactggtacc acggcggctt catgagcgta
     7141 agcttcaacg ggctgaacct gggcaacatt gcgccggccg tgctgagggt cgtggagcag
     7201 gggcagcggg ggctcatcga gctactgtgg aagaccgcgg aggggcaggt gcggatacgg
     7261 ttcctgcagg agcccgaggc tgactacctg gcggccgagc ttgcggtgct tccaacgagc
     7321 gagatccgca gcctggacct ttccctacgc tgctacccca gctacttcac cggttggaac
     7381 aagcgcgacg gctggcgaca gatcatcggt ccgatgacga cgaccgagca gggacagacg
     7441 aaggcgctgg acccgctgca ggactggtgg ctgctctacc aggatcgcgt cttcgattac
     7501 gccctcaacc gcgacgacag cgccggcccg tgcgggctgc tctacctccc cgaacagatc
     7561 aagagcgtgc aggttctccc caccagctac cccgtcacca cgaacgtcac ggtgaagccg
     7621 acggtgcggt cggtgcgcct ggccttctgg gagttcccca agaagacgaa cgccgagagc
     7681 ctgcagcggt tgcggcaggg ggcggcccag gttgcagagc acctgcgcca ggtggacttc
     7741 agcgacagcc aggtggccgc cttcgacgcc gcaggcgaac gcgccgccct ggcggcgatg
     7801 atcgcgaaga ccaaagagcc ggaggcgtgg cggcagaggc tggcgccgat gcttgatcag
     7861 gtggcggcgg cgcagcaggc ctaccgcagc aatgacctgt cggcggagca gacggcctcg
     7921 gcggctctgg cgaagtaccg ggacgcggtc tgggacctga agttcgaagc actactgagt
     7981 gactgagaga gacggagaga gtgaaccacg aaatacacga aagaacacga aaaacggcga
     8041 tactgggaaa gacatcaggc agctcctttg agcggtgaga gaggggcgca gtcggggctg
     8101 gaagcccctc ccacgggtgg cctgcggcag cctggtcgtg caactcgtct ctcgacgtcc
     8161 ttcgttcgct tccgtatctc tcgctgtctc gccgtctttc gtgttctttc gtgtttttcg
     8221 tggttggttg tcgtttctcg tgtgactgct cgcctgcggg aggacctcat gcggtcgatc
     8281 tgtgctgtgg taatgctggt ggcgtgcctg ttggtagtgc cggcgggggc ctacgtgatg
     8341 gagaaggact tctatgccct ctccgaagat tacgtgtcgc cgcacattgc ctggggcaag
     8401 ccgtatgccg gcggaaagat caaagccctg ttcatctgcc cgcgggggga tgcgcgggaa
     8461 gtggtcgagg tggcggagcg gctcgacctg gactacaagg tggtcatgac cctgagcagc
     8521 gatgagctcg ggtggacctc caagtctggc ccgtatgccg gcgcggaagg catctccaac
     8581 gatgacatga tccgcgagac cgccgagaag ctcaagggca actacgacgt gatcatcacc
     8641 gggcagatca agtgggacat gttcccgcgg gagctgctct acacgatcat ggagaaggtc
     8701 cacaacggca cagggcttgt ggtgggctac agcgactacg gtcgcaatgc cctggtggac
     8761 cggctgttcg cgaagccgga ggtgccgggg gagtatgtga cgctgggggt gccgctgaag
     8821 gcgctgccgg tgctggacaa gctcgacccg aagcaggcga ttcagacgcg ccagttccat
     8881 gacggaagga tggtgctgct caagtacccg gggcgcccga tcttcatgtt tctcaccccc
     8941 ctgccgccgg acacggatga cagctaccgc gaattgcact acgagtacta ccagtcgctg
     9001 gtgctcaagt ccatcctgtg ggcggcgaag aaggagcccc aggtacagtt caacgatctg
     9061 ggcgtcggag gagacacgat caaccgcacg aactcccact gcgtcggctg gtggaagtac
     9121 acattgacca tcgtgaaggc ctgcatcagc cgcgaccagt cgttacccga gaagctgttc
     9181 gtgtcgaagg ggccgtcgaa gcccacctcc gcctccgggt cagccttgcg gatcgcctca
     9241 ccggcccgct gcatcatccg cgcgtagacg aactccatgt gccgccggtg gtccacccac
     9301 tgcactgggc gaccggcctt cttcgcgtcc tgcaaggtga tcggctgcgc ctcaccaaag
     9361 ccgccgtagt tggtgcccca ggaggcattc agcgctgcga tgttgccgta ctcgccctgc
     9421 agccactgct ggaagtcggc gatgcaggtg ggggagaagc agacgtcaac gccgtcacgg
     9481 gcgaggaagc actcgtcacc gagggtatac gcccgcggcc ccatcggccc aagctcccgc
     9541 ccaatcttct ccaggctctc caggtgcggg cccaggaact tcgggtccgt caggcacggc
     9601 tcacgcaccg tattgctgcc ttcatacgag tagcgggtgg cgtaagggat ggtgtcgaag
     9661 ttgttgctct gcagccacgc ctgccccggg acgtttgtcg gcgcattcgt ctgcaggtcc
     9721 acatcgcagg cccgcagttg ccgcagcatg atccgccgca cgaagtcatt gcgctgcccg
     9781 tccgcactcc acaccgcgtg ggcatagttg ccgcggctcc gcttcagagg tacgaagagc
     9841 tcccggctgg ccttcgccac gcacgcctgt tcgagaggct gcttccccgg cgacccgttt
     9901 accaggtatg ccgtcacccg ggcgctcagg gccaacgggt ccgcgagttg gaacgggacc
     9961 ttgatctcct tgctgcccgc cgcgaccgtc acgctctttc gtacgatcag ccggtccagg
    10021 ttgtctacga cctctaggta caggttcatg tctgcggggg ccgaagctga tagcgtcgca
    10081 gtggctgtga ctgtctcgtt cgccttcgcc gccggcttgt cgagcgacag cgcagtgatg
    10141 ttcaggtcag acaccaccgt aaagcccacg ctgccccaat tcacgcagtt ctgccccgct
    10201 cggatgatga catcggcgaa gtacttcccc gccggcagca ccggcagttt cacactcagg
    10261 tccttgccgg ggatctcctg cgccgtcttc agaaagagtg tcttgtcctc atcccgcacg
    10321 gtcacgtgcg cctgcatgcc gttggtcgcg ccgcgccagt gggcggtaag ctgcttcccc
    10381 gtcagctccg cgcggttgat cgtgtctcct ccgacgccca gatcgttgaa ctgtacctgg
    10441 ggctccttct tcgccgccca caggatggac ttgagcacca gcgactgg
//
LOCUS       DATALS010000501         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_309004, whole genome shotgun sequence.
ACCESSION   DATALS010000501 DATALS010000000
VERSION     DATALS010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309004"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VHS06_11180"
     CDS             complement(<1..334)
                     /locus_tag="VHS06_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2988718.1"
                     /translation="MGVIYATAKAAVAEYSRCLAMQLRPYNVMVNVVAPGPILTPRFA
                     ASRPLEEGMMAETGALVRYGRAPEIAAAVAFLISAERLSGLFQPYHRIQEARRHRESL
                     GLGLYVTKG"
     gene            complement(430..2646)
                     /locus_tag="VHS06_11185"
     CDS             complement(430..2646)
                     /locus_tag="VHS06_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988719.1"
                     /translation="MTYATRSARDYRKAELACLQIIADIIGSGLENAWLHTRLEAAIL
                     QAQGLAEKARAATCTLLALIDTMPAGVVVVDREGNINISNAAAGEMLGGEVTGTAFGP
                     HGRYTLHRVDGSPLPSEELPLRRALEAGEASKNVEVLVQLEDGSRSVILTGSAPVRDE
                     QGDVAGAVSVFLDVTEQKRAEEGRVRLAQEQAARAQIEAHAQRLETVIQAVPDAMVVA
                     DRQGNVVTANDAFVKLIGLANQRGALKPLAEHWKSTRVLTLDGRPLSIEESPTLRALQ
                     GEKVLLLEARLQLADGRERLVEVSSAPVFDDHGEVTHSVTVLRDVTEQRRWQHQRNLL
                     ARVGQALSCTLELKSVLQAVMEETLQGIGADGVAIFLTGPDRQQLQLAASRGFRQETE
                     GLLRQFPMDRSLATVRAFETGEIQIIEDLERASQDSEWIRAASAVEGVSSVVAVPLRA
                     HGVTIGVMTFVSRPPRHFMPDELDTIARVAELFAMAIENARLFEEAERRADSLHELQE
                     QRTKYILGISHGLRTPLTAIQGQAQLLLRSLQKAGMNGRLQHSAETVVASSRRMSVML
                     RDLVDLMHLEARQRLRLNLEPIELPSFIADVIGKLDGVLEAGRVKIEAPESFPQVMAD
                     PDRLERILINLLSNALEYSDPGTEVKVKLEQRDGEVIVSIEDRGKGISAERLSGLFQP
                     YHRIQEARRHRESLGLGLYVTKGLVESHGGRIWAESEVGKGSRFSFTLPLSQPQAR"
     gene            complement(2603..3073)
                     /locus_tag="VHS06_11190"
     CDS             complement(2603..3073)
                     /locus_tag="VHS06_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988720.1"
                     /translation="MGVAGPRGRLTQERQLRDAIGQVALQMAEQLDWDKVVETVLHVT
                     STALGADAVIIWNVDVEARLLHLAASWGVPEPRVRPLRHLSFDSPLMVARAVHEGRVQ
                     MVERLDELPSESPRPSTMDGPWVSERGGPAPAISRPVGRCDDLRHPLRPRLPQG"
     gene            3325..>4011
                     /locus_tag="VHS06_11195"
     CDS             3325..>4011
                     /locus_tag="VHS06_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX2988721.1"
                     /translation="MTKPRTIHELRAGDYRVLSVKEELRKNLIEKLRRGENPFPGLVG
                     YEETVVPQVENAILSGQDIVLLGERGQAKTRLARSMVSLLDEDVPAMADCEINDDPYH
                     PICQQCRTKLDEEGDDAEIVWIGRDRRYGEKLATPDITIADLIGEVDPIKVAEGRYLS
                     DELTIHYGLIPRTNRGIFAINELPDLAERIQVGLLNVMEERDVQIRGYKVRLPLDLYV
                     VATANPEDYTN"
BASE COUNT          691 a         1331 c         1248 g          741 t
ORIGIN      
        1 gccccttggt gacgtagagc cctagtccga ggctctctcg atgacggcga gcctcctgga
       61 tccggtggta gggctggaac agtccgctga gtcgctcggc ggagatgaga aaggcaaccg
      121 cggcagcgat ctccggggcc cggccgtaac ggaccagcgc cccggtctcc gccatcatcc
      181 cctcctccag cggccggctg gccgcgaagc gcggggtgag tatcgggccg ggggccacga
      241 cgttcaccat cacattgtag ggtcgaagct gcatggccaa gcagcgagag tactcggcga
      301 cagccgcctt ggcagtggcg tagatcaccc ccaccgtcgt ccctcccagg ccggcgatgc
      361 tgcgggtgga cggcggccac cagtgctggc cggcctagca ggataccggt cgccgatcca
      421 ccgacgcttc tatcgggcct ggggctgaga cagcggaagg gtgaatgaga atctgctgcc
      481 cttccccacc tcgctctcgg cccagatgcg tcccccgtgg gactccacaa gccccttggt
      541 gacgtagagc cctagtccga ggctctctcg atgacggcga gcctcctgga tccggtggta
      601 gggctggaac agtccgctga gtcgctcggc ggagatgccc tttcctctgt cctcgatgga
      661 gacgatcacc tccccgtctc gctgctccag ctttaccttc acttccgttc cgggatcgga
      721 gtactccagg gcgttgctga gcaggttgat cagtattcgc tccagccgat cagggtcggc
      781 catcacctgg ggaaacgact ccggggcttc gatcttgacc ctgccggcct cgagcacccc
      841 atcgagcttg cctatcacgt ccgcgatgaa gctcggaagt tctatcggct ccaggttcag
      901 cctcagccgc tgtctagcct caaggtgcat gagatccacc agatcgcgga gcatcacgct
      961 catcctccgc gacgaggcga ccacggtctc ggcgctgtgc tggagccgcc cgttcatccc
     1021 cgccttctgc agcgaccgaa gaagaagctg cgcctggccc tggatggccg tcaggggggt
     1081 cctgaggcca tgggagatgc cgaggatgta cttggtgcgc tgctcttgca gttcgtgcag
     1141 agagtccgcc cggcgctccg cctcctcgaa caggcgtgcg ttctcgatgg ccatggcaaa
     1201 cagttcggcc acgcgggcta tggtgtccag ttcgtccggc atgaagtggc gtggtggacg
     1261 ggagacgaag gtcatgacgc cgatggttac cccgtgcgct cgtagcggga cggccaccac
     1321 cgagctgacc ccttccacag ctgacgcggc gcggatccac tctgagtcct gactcgcccg
     1381 ttccagatcc tcgatgatct gtatctcccc cgtctcgaag gctcgcaccg tcgcaaggga
     1441 tcggtccatg gggaactggc gaagtagccc ctcggtttcc tgccggaatc cacgagaggc
     1501 cgccagttgc agctgctgtc ggtccgggcc ggtgaggaat atcgccactc catcggcccc
     1561 gatcccctgc agcgtttcct ccatcactgc ctgcagcacc gacttcagtt ccagcgtgca
     1621 gctgagcgcc tgccctactc gcgccagcag gttgcgctga tgctgccacc ttcgctgttc
     1681 ggtcacgtcc cgaagcaccg tgaccgagtg ggtcacctcc ccgtggtcgt cgaacacagg
     1741 ggcggagctg acctccacca accgctctct gccgtccgcg agttgcaggc gcgcctccag
     1801 gagcagcacc ttctctcctt gcagcgcccg gagggtgggc gactcttcga tagacagcgg
     1861 cctcccatcc agggtgagta cgcgagtcga tttccagtgc tcggccaatg gcttgagcgc
     1921 ccctcgctga ttggcgagcc caatgagctt cacgaaggcg tcgtttgccg tcacgacgtt
     1981 gccttgcctg tcggcgacga ccatcgcatc gggcacggcc tggatcacgg tctccaatct
     2041 ttgagcgtgc gcctcgatct gcgccctggc ggcctgctcc tgcgccagcc gcacgcgccc
     2101 ctcctctgcc ctcttctgct cggtgacgtc caggaacacg gagaccgccc ctgctacgtc
     2161 gccctgctcg tcgcgcacgg gggcgctacc ggtcagaatg acggatcggc tgccgtcctc
     2221 cagttggacg aggacctcca cgttcttcga tgcctcgccc gcttccagcg ccctgcgtaa
     2281 cgggagttcc tccgatggga gagggctgcc gtcgactcgg tgaagtgtgt agcgcccatg
     2341 aggaccgaag gcggttcccg tcacttcacc gcccagcatc tccccggccg ccgcgttgga
     2401 gatgttgatg tttccttctc tgtcgacgac gacgaccccg gcaggcatcg tgtcgatgag
     2461 ggccagcagt gtgcaggtgg ctgcccgcgc cttctctgcc aatccttggg cttggaggat
     2521 agcagcctcc agccgggtgt ggagccatgc gttctcgagt cctgatccga tgatgtcggc
     2581 aatgatctgt aggcatgcca gctcagcctt gcggtagtcg cgggcggagc gggtggcgta
     2641 ggtcatcgca ccgaccaacc ggccgcgaga tcgcaggggc agggccacca cgctctgaaa
     2701 cccaaggccc gtccattgtt gacggacggg ggctctccga agggagttca tccagccgtt
     2761 cgaccatctg aacccgcccc tcgtgaactg ctcgagctac catcaggggg gaatcgaagg
     2821 acagatgccg gagtggccgg accctgggtt cgggaacccc ccaggaggca gccagatgaa
     2881 ggagtcgggc ctcgacgtcc acgttccaga ttatgacggc gtccgccccc agggccgtcg
     2941 atgtgacgtg tagaaccgtc tcgacgacct tgtcccagtc caactgctcg gccatctgca
     3001 gcgccacctg gccgatggcg tctctcagtt gtctttcttg ggtgagcctg ccgcgaggac
     3061 ctgcaactcc caacagtcag ctcctctttg ggacggaagg gcgtcttgct tctacggcgt
     3121 ccaccactgt gcactgcatg gggaatacca cagcagtgga cgtggcgaag gttcgcagcg
     3181 caaattaaag ctatatacaa tacaaatata gcgctatact atagggctcg agcgggacgg
     3241 ggcgaccgct cgggtcgtgg ctccactgct gcacttcttg cggtaagtgg gtgagcctca
     3301 cctaatgaac agggagcagc cgtcatgaca aagcctcgga ctattcacga gctgcgcgcc
     3361 ggcgactatc gtgtcctctc agtcaaagag gagttgcgca agaaccttat cgagaagctg
     3421 cgccggggcg agaacccctt tcccggcctt gtgggctacg aggagactgt ggttccccag
     3481 gtcgagaacg ccatcctctc cgggcaggac atcgtcctcc tgggagagcg cggccaggcc
     3541 aagacccgcc tcgcccggag catggtgagc ctcctggacg aggatgtgcc tgccatggcc
     3601 gactgcgaaa tcaacgatga tccctaccat cccatctgcc agcaatgccg aacgaagctg
     3661 gacgaggagg gggacgacgc cgagatcgtc tggatcggcc gcgaccgtcg ctacggtgag
     3721 aagctggcga cgccggacat aaccatcgcc gacctgatcg gggaggtgga ccccatcaag
     3781 gttgcggagg ggcgctatct ctccgacgag ttgaccatcc actacggcct catcccacgg
     3841 accaatcggg ggatcttcgc catcaacgag ctgccggacc tggccgagcg gatccaggtg
     3901 gggctgctga acgtgatgga ggagcgcgac gtccagatcc gtggctacaa ggtccggcta
     3961 cccctggatc tctacgtggt cgccacggcc aaccctgagg actacaccaa c
//
LOCUS       DATALS010000502         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_122726, whole genome shotgun sequence.
ACCESSION   DATALS010000502 DATALS010000000
VERSION     DATALS010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122726"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..868
                     /locus_tag="VHS06_11200"
     CDS             101..868
                     /locus_tag="VHS06_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988722.1"
                     /translation="MLLTFERGDSQRPKGHALLYFRSWTDRSTVFATYLIVPPIAVEL
                     TKYIPPMFAGQFQQMGLGAGGISVFPYPPIPERVESHALLQRLAGARDDDLVDGGSVD
                     ASSPERLLQAASEAAAQYFQQFSSYMESVPTETAPEALPEEGSSSVDVNEILYGLMSE
                     RDRLAELAKLSGQLRYAVSSNDPSLLEETVAEVHRLAKFLPEKYRVEQVVKAAQRTGG
                     QGDRLTQLYLDRAYKLIDEDYQAVKRIEDEIRGEEAA"
     gene            865..2181
                     /gene="pyk"
                     /locus_tag="VHS06_11205"
     CDS             865..2181
                     /gene="pyk"
                     /locus_tag="VHS06_11205"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX2988723.1"
                     /translation="MRLARIQATLGPACSDLDTLRKMVEAGMDGCRLNMSHCSSGQAR
                     ELAGMVRRISKEKGRPIALGADLRGPKLRIGDVEGSGVVLAQGSHVELTPEDRVSVPG
                     LLSVNYPYLSEDVRPGDPILLNDGALRLRVEATVRDRVVCRVEKGGLLTSRKGVNLPG
                     VPLRVPSLTEKDVKDIALAVELGVDFLYVSFARSRGHIMDVRKALQRLGSSIPLVAKI
                     ERQEGVDALSEIVDAADGICVARGDLGVETPLGAVPFIQREAAGLCHAKGRFAMMGGQ
                     VLASMVSSPTPLRAEVADLATIVRDGNDAIVLSDETAAGEYPVEAVRVAAQVMERTEQ
                     SAGQNGTTWGHQAGQGHSIGSVVVVTSSGEAVKRLSTTRSAAPIVAAVDRADLANWLS
                     TWWGVVPVLLDNLSDPAEAARLALKRAAEVEPSLAGIRPLVLCELS"
     gene            complement(2411..>2791)
                     /locus_tag="VHS06_11210"
     CDS             complement(2411..>2791)
                     /locus_tag="VHS06_11210"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2988724.1"
                     /translation="AGAFPPIVVTTRMASQQQIAALEPHAHLMVAGEEQVDPHLMMRM
                     LFDEHRIRRLVVEGGPTLNGVLLGQGLVDELFWTLAPKLVGGPALHTMIEGNALPIDN
                     LARLELLSLHHHENELFLRYRVLS"
BASE COUNT          536 a          819 c          940 g          496 t
ORIGIN      
        1 ggggcgagat gacgaggtgg ggatgggttc gcaccgcggc tcaacagatg gcgtataatg
       61 gattaggtga atacccaagg ggggaggcta ggtatccgct atgctgctca ccttcgagag
      121 gggcgattcc caacggccca agggccatgc cctgctttac ttccgcagtt ggaccgatag
      181 atccaccgtc ttcgcgacct atctgatcgt acccccaatc gccgtggagc tcaccaagta
      241 catacctccc atgttcgccg ggcagttcca acagatgggg ttgggtgccg gtgggatcag
      301 tgtatttccc tatcctccca ttccggagcg agtcgaaagc cacgcactgc tgcagcggtt
      361 ggccggcgcc cgagacgacg acctggtcga tgggggatct gttgacgcgt cctctccaga
      421 acggcttctc caggcagcaa gcgaggctgc ggcgcagtac tttcagcagt tctccagcta
      481 catggaatcg gtgccaaccg agacagcgcc ggaggcactg ccggaagagg gatccagctc
      541 agtcgatgtg aacgagatcc tctatggact catgagcgaa cgggacaggc tggctgagct
      601 ggccaagctg agtggacagc tgcggtacgc ggtcagcagc aacgatccta gcctgttgga
      661 ggagacggtt gccgaggtcc accgcctggc gaagttccta cccgagaagt accgggtgga
      721 gcaggtagtc aaggcagccc agcgcacggg cgggcagggg gaccggctga cacagctgta
      781 tctggaccgg gcctacaagc tcattgacga ggactaccag gccgtcaagc ggatcgaaga
      841 cgagatacgt ggagaggaag ctgcatgaga ctggccagga ttcaggcgac gttggggccg
      901 gcgtgctccg acttggacac cctgcgaaag atggtcgaag ccggaatgga tggatgccgg
      961 ctcaacatgt cgcactgctc ttccgggcag gcgcgagagc ttgcaggcat ggtgcgccgc
     1021 atatccaagg agaaggggcg accaattgcc cttggggctg accttcgcgg cccgaagcta
     1081 cgcattgggg atgtggaggg aagcggcgtg gtgctggcgc aggggagcca tgtggagctt
     1141 actccggagg accgcgtttc cgtgcccggg ctgctctccg tcaactatcc ctacctgagt
     1201 gaggacgtcc ggccgggcga cccgattctg ctgaacgacg gggcgctccg cctgcgagtc
     1261 gaggctacgg tgcgggacag ggtggtgtgc cgggtggaga agggtggcct tctcacctct
     1321 cgcaaggggg tcaacctccc gggtgtcccg ctcagggtgc catcgctcac cgaaaaggat
     1381 gtgaaggaca tcgcccttgc agtggaacta ggggtagatt tcctctacgt gtccttcgct
     1441 cggtctcgcg ggcacatcat ggatgtgcgc aaggctctcc agcggctggg gtcttcgatc
     1501 ccactggtgg cgaagatcga gcgtcaggag ggggtcgatg ccctgtcgga gatcgtggac
     1561 gccgcagacg gcatctgcgt tgcacgcgga gacctgggtg tagagactcc tctcggcgcc
     1621 gtgcccttca ttcagagaga ggcggcgggc ctgtgccatg ccaaaggaag attcgcgatg
     1681 atgggaggac aggtcctcgc cagcatggta tcgagcccta cacctctgcg cgcggaggtg
     1741 gccgacctgg ccacaatagt gcgggatggc aacgacgcca ttgtgctttc cgacgagacc
     1801 gcggctgggg aataccccgt ggaggccgtg cgggtggcag cacaggtgat ggagcgtacc
     1861 gagcagtccg ccgggcagaa tggcacgacg tgggggcacc aggccggcca agggcacagc
     1921 atcggttcgg tagtggtggt cacatcgagc ggggaggcgg tgaagcggct ttccaccacc
     1981 cgttcggctg ccccgatcgt tgcagcagtg gaccgggctg acctggcaaa ctggctctct
     2041 acctggtggg gagttgtgcc ggttctgctg gacaatctgt ctgaccccgc cgaggcagcg
     2101 cggttggcgc tgaagagggc agcagaggta gagccgtctc tggccgggat caggccgctg
     2161 gtactgtgtg agctgagcta acccccggcc ccccttccct acgcgggaag gagggagcaa
     2221 acgagttccc tctccctcag ggagaggcgt gatgctgtgc cagctcgcat cacggggtga
     2281 gggaagtacc agttggtgag agccgacttg aagtgaaggc aacaggctcc cctcacccca
     2341 accctctccc caccgcagtg gggagaggga gtacactcct ccccgtctcc ccttgaggaa
     2401 cggtttcccc ctagcttagc acgcggtatc tgagaaacag ttcgttctcg tgatggtgga
     2461 ggctgagcag ctccagacgc gccaggttgt ctatcggcag cgcgtttccc tcaatcatcg
     2521 tatgcagcgc cggccccccg acgagtttgg gggcaagcgt ccagaacagc tcatccacca
     2581 acccctgacc caacaggacc ccgttcagag tagggccacc ctccaccacc aaacgacgga
     2641 tgcgatgctc atcgaacagc atgcgcatca tgagatgagg atccacctgc tcctccccgg
     2701 cgaccatcag gtgagcatgc ggctccaggg ctgcgatctg ctgctgtgag gccatcctcg
     2761 tggtcaccac tatgggtgga aatgctccag c
//
LOCUS       DATALS010000503         6023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_74590,
            whole genome shotgun sequence.
ACCESSION   DATALS010000503 DATALS010000000
VERSION     DATALS010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74590"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VHS06_11215"
     CDS             <1..573
                     /locus_tag="VHS06_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HEX2988725.1"
                     /translation="ARFLAEDGAKIIAVSDSKGGIYNPNGLNPWDVIRYKEETGSVVG
                     APRSQSISNAELLELECDILIPAALEKQITDKNAPRMKTKLIAEAANGPTTPEADEIL
                     TANGTLIIPDILANAGGVTVSYFEWVQDLMAFFWTPEEINAKLETIMVKSFRDVKKVA
                     DDYGVTMRIAANMLAIKRVAEASTIRGIYP"
     gene            complement(792..1913)
                     /gene="ugpC"
                     /locus_tag="VHS06_11220"
     CDS             complement(792..1913)
                     /gene="ugpC"
                     /locus_tag="VHS06_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX2988726.1"
                     /translation="MAQVVLDHVTKRFGSVTAVNDLCIEIEDKEFLVLVGPSGCGKTT
                     ALRMVAGLEEITSGNIYIGDRLVNDVSPKDRDIAMVFQSYALYPHMNVYDNMAFGLKM
                     RKFSRDEIDKRVKVAAGILGIEMLLDRKPKQLSGGQRQRVALGRAIVREPKVFLMDEP
                     LSNLDAKLRVQMRAELIKLHQRLQTTIIYVTHDQIEAMTMGSRIAVMRDGVLQQLDTP
                     QKLYQDPANVFVAGFIGSPAMNFFEVQITTEGGTVTLTGEGFSASLAGTRAQAVAPYN
                     GKRVVVGIRPEHLAEVSSGETDETGELVAQVEVVESMGSEQHLYLLVGGKSFVARVDG
                     YFKARVGDQIKFAFPKEQMRLFDKDTERAIVRAAEMATV"
     gene            complement(1920..2765)
                     /locus_tag="VHS06_11225"
     CDS             complement(1920..2765)
                     /locus_tag="VHS06_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2988727.1"
                     /translation="MVGGPGALRKFLILYLPMILYAVLLLFPFYWMAVVSLRPNADLF
                     NTKMNPFWPWNATLENYQYLFEKTGFPSWTINTSIVTFASTFLSLFCSILIGYALGRL
                     RFRGANFMGLVIFLAYLVPPTLLFLPLAQVIASLHLYNTYWALILTYPTFLVPFGGWI
                     LMGYFRTIPHELEECAMIDGASRLRAMVSIVLPLALPGILSAGIFSFTLSWNEFLYAL
                     IFMSSGTMKTIPVGTVSDLIKADVYQWGALMAAGLLGSVPVAVIYSFFVDHYVSGLTA
                     GAVKG"
     gene            complement(2767..3717)
                     /locus_tag="VHS06_11230"
     CDS             complement(2767..3717)
                     /locus_tag="VHS06_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2988728.1"
                     /translation="MSMVQPSALDGARLSKPPAPRRRLGDLLEREGSLGWLLMAPGSL
                     LLLIFIAYPFFLGIWLSLTNSMVGKMGAFVGLKNFIFLLMSDSIFMQSAMNTFVYAFI
                     SVPFKLFLGLGLALLLNQQFRFRNALRAGLLLPWIVPTALSSLAWLMIFDGVLSPISW
                     LLRALGLASANIPFLGDPFLAMSALVFANVWRGVPFFGISILAGLQAVPAELHEAAAI
                     DGAGPWQRFLAVTLPVIRGITLIATLFSIIWTFADFQLIYVLTKGGPANKTHIFGTYA
                     YQIGLNATDIGQGAAIALWMFPFLAVFAAILLMYLRKGDE"
     gene            complement(3859..5274)
                     /locus_tag="VHS06_11235"
     CDS             complement(3859..5274)
                     /locus_tag="VHS06_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2988729.1"
                     /translation="MEFEKDGISKKRLTRRQMLLALGAGAASIAAAACAGAPAAAPTA
                     APKAAEPTKPAASGAAPAAPAATTAPAAAPAVLKGTSLAFLGGTYFVPAAQKLFVDQM
                     NEWGKANGVTVSADFLSWPDLQAKIAAAIQAGGGTDIFDFWPGWSYLYADNLVDMTDF
                     VTQIETRDKGFYDWVTKSTKAKGKWWSVPIGTTNAALNYRISYLKQVGADKFPDTWDE
                     LFAVGKKLKAMGKPIGQALGHSTGDPPGLAYPYMWSFGAMEVMEDGKTVAFNKPEFVD
                     AMKRFIQGWKDAFDTTGTSWDDSANNRAFISDQISITYNGSSIYETAKKDAPKIAEDM
                     DHADMCAGPSGRFYQLGSRSFGVLKNSKNVAGAKDFLNWWLDPKRFDEWIRIQGGYQL
                     PPSKRWEGDPLLIKDPKYAAFARQAKFGRTVGWAGELNEKAALSFSKYIVVDTFAKAV
                     TDGDAAAAIKWGADQLQQIYG"
     gene            5826..>6023
                     /locus_tag="VHS06_11240"
     CDS             5826..>6023
                     /locus_tag="VHS06_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988730.1"
                     /translation="MIIKNWDELTSHGDRRGRETALAILEAGLQAADPYANVKKLVRL
                     DGDTLRVGQSEMDVSGFGEEVI"
BASE COUNT         1188 a         1758 c         1887 g         1190 t
ORIGIN      
        1 gcgaggttcc tggctgaaga cggtgccaag atcatcgcgg tgagcgactc aaagggcggc
       61 atctacaatc ctaacgggct caatccgtgg gacgtcatcc gatataagga ggagacgggg
      121 tccgtcgtcg gcgctcctcg cagccagtcc atcagcaacg cggaactgct ggagctcgag
      181 tgcgacatcc tgatacctgc cgctttggag aagcagatca ccgacaagaa tgcgcctcga
      241 atgaagacca agctgattgc cgaggccgcc aacggcccga caacgcccga ggcggatgag
      301 atcttgacgg cgaacggaac gctgatcatc cccgatatac tggccaacgc aggtggtgtg
      361 acagtctcct actttgagtg ggttcaggat ctgatggcct tcttctggac cccggaggag
      421 atcaacgcca agctggaaac catcatggtg aagtcattcc gcgatgtaaa gaaggtggca
      481 gacgactacg gcgttaccat gaggattgcc gccaacatgc tggccatcaa gagggtagcc
      541 gaggcatcga ccatccgcgg catctacccg tgatctaagc cgcgaccgtc taccgaaccg
      601 aagcactggg aggaagcgtt cgtcggcgac tgtgaatgga gcgaaggctt cagccggaga
      661 gaacgggccg ggtcagggac gacccggccc tcctgattct gggagccggg gtcgtccccg
      721 caacgcgagc gagtgtagcg ttgcgcccgg cggtttcggc tgaaggcttc actccatttg
      781 gtgttcagtt gctagacggt ggccatctct gccgccctga cgatcgccct ctcggtgtcc
      841 ttgtcgaaca gcctcatctg ctcctttggg aaggcgaact tgatctgatc acctacgcgg
      901 gccttgaagt agccgtccac ccgagccacg aagctctttc ctcccaccaa caggtacagg
      961 tgctgctcgc ttcccatgct ttccaccacc tctacctgtg ccaccagctc accagtctcg
     1021 tcggtctccc cactgctcac ctcggcaagg tgctcgggcc tgatccccac cactacccgt
     1081 ttcccgttgt aaggggccac cgcctgggcc ctcgtccctg ccaggctcgc gctgaaacct
     1141 tctcctgtca gggtcaccgt cccgccttcg gtcgtgatct gcacctcgaa gaagttcata
     1201 gcggggctgc cgatgaagcc cgccacgaat acgttggcgg ggtcctggta cagcttctgg
     1261 ggagtatcca gctgctgcag caccccatca cgcatcaccg caatccggct gcccatggtc
     1321 atagcctcga tctggtcgtg ggtcacgtag atgatcgtcg tctgcagcct ctggtgaagc
     1381 ttgatcaact cagcgcgcat ctgcacgcgc agcttggcat ccaggttgga gagcggctcg
     1441 tccatgagga ataccttcgg ctcccgcacg atcgccctgc ccagtgccac gcgctgtcgt
     1501 tgtcctccgg atagctgctt cggcttgcgg tccaggagca tctctatccc gaggatgccg
     1561 gccgccacct tcactcgctt gtcgatctcg tcccgagaga actttcgcat cttcaagccg
     1621 aaggccatgt tgtcgtacac attcatgtgt gggtacaggg cgtaggactg gaagaccatg
     1681 gcgatgtccc gatccttcgg tgagacgtcg ttgaccaggc gatcgccgat gtagatgttg
     1741 ccgctggtga tctcctccag ccccgccacc atccgcagag cggtggtctt gccacagccg
     1801 gaagggccca ccagtaccag gaactccttg tcctcgatct cgatgcacag gtcgttgaca
     1861 gccgttacgc tgccgaacct cttagtcaca tgatccaata ctacttgtgc catctctccc
     1921 tatcccttca cagcgccggc cgtgagaccg gaaacgtagt ggtctacgaa gaaggagtag
     1981 ataaccgcca ccggcacgga gccgagtagg ccagccgcca tcagtgctcc ccactggtag
     2041 acatcggcct tgatcaggtc gctcacggtc ccgacgggta tggtcttcat ggtccctgaa
     2101 gacatgaaga tcagcgcgta caggaactcg ttccaggaga gcgtaaatga gaagatgcct
     2161 gccgagagga tgcccggcaa ggccagcgga aggacgatgg agaccatagc ccgaagccgg
     2221 ctggcgccgt cgatcatcgc gcactcttcg agttcgtgtg gaatggtgcg gaagtagccc
     2281 atcaggatcc agcctccgaa gggcacgagg aaggttggat aggtcaggat gagcgcccag
     2341 taggtgttgt agaggtggag agaggcgata acctgggcga gtggcaggaa caggagggtc
     2401 ggtggcacca ggtaggccag gaagatcacc agacccatga agttcgcgcc ccggaagcgg
     2461 agcctaccga gggcgtagcc gattagaatg ctgcagaaca gcgacaagaa ggtcgatgcg
     2521 aaggttacga tgctcgtgtt gatggtccag gaagggaagc cggtcttttc gaacaggtac
     2581 tggtagttct ccagtgtggc gttccatggc cagaaggggt tcatcttggt attgaagagg
     2641 tctgcattgg gcctcagcga taccaccgcc atccagtaga aggggaacag caagaggacc
     2701 gcatacagga tcatcggcag gtacagtatc agaaacttgc ggagagcgcc cgggccaccg
     2761 accatcttat tcgtctcctt tcctgaggta catcaacagg attgcggcga atacggccag
     2821 gaaggggaac atccacaatg cgatggccgc gccctggccg atatcggtgg cgttgaggcc
     2881 gatctggtag gcgtaggttc cgaagatgtg ggtcttgttg gccggccccc ccttggtcag
     2941 aacgtagatg agctggaagt ccgcgaatgt ccatatgatg gaaaagagag tggcgattag
     3001 ggtgattcca cggatcaccg gcagcgtgac ggcgaggaat cgttgccacg gtccggcacc
     3061 gtcgatggcg gcggcctcgt gcagctccgc gggcactgct tgcagaccgg ccaggataga
     3121 gatcccgaag aagggcacgc cgcgccagac gttggcgaag accagtgccg acatggctag
     3181 gaacggatct ccaaggaatg ggatgttcgc gcttgccagc cccagcgccc ttaagagcca
     3241 ggagatggga ctcaacaccc cgtcgaagat catcaaccac gcgagactgc tgagcgcggt
     3301 tgggactatc catgggagga gcagacctgc tcgtagcgca ttgcgaaatc gaaactgctg
     3361 gttcaacaac agtgccagcc ccaggccgag gaacagcttg aacggcacgc ttatgaaggc
     3421 gtacacgaag gtgttcatcg cggactgcat gaagatgctg tcactcatca agagaaagat
     3481 gaagttcttc aaccccacga aggcgcccat cttgccgacc atggagttgg tcagggagag
     3541 ccaaatgccc aggaagaacg gataggcaat gaagatgagc aggagcaggc tccccggcgc
     3601 catcagaagc cagccgagag aaccctcacg ctccagaagg tcgcccaacc gccttcgtgg
     3661 agccggcggc ttggagagac gtgcgccgtc cagggcgctg ggctgtacca tactcatgtg
     3721 gtgatccctc cgcaaagagg gccgcgcgcg atgccgtcga ggccgcgatg cgccggtccc
     3781 agagtagttg ccttttggtg cagggcagca gtcaaagggt tggggagcgc ggcgcatttg
     3841 cgccgcgctc ccgcgactct atccgtagat ctgctgcagc tggtcggcgc cccacttgat
     3901 cgccgccgcg gcatcgccgt cggttactgc cttcgcaaag gtatccacga ctatgtactt
     3961 ggagaaggag agcgcggcct tctcgttcag ttcgcccgcc catccgacgg tgcgaccgaa
     4021 cttcgcctgg cgggcgaaag ccgcgtactt cgggtctttg atcagaagag ggtcaccttc
     4081 ccacctcttg ctggggggaa gctggtagcc accctggatg cggatccact cgtcgaagcg
     4141 cttcggatcc agccaccagt tgaggaagtc cttggcaccg gcgacgttct tggagttctt
     4201 gagaacgccg aaggagcggc taccgagctg gtagaatcgg ccggatggac cggcgcacat
     4261 gtccgcgtgg tccatgtcct cggcgatttt tggggcatcc ttcttggcgg tctcgtagat
     4321 gcttgagccg ttgtatgtga ttgagatctg atctgagatg aaggctcggt tgtttgcgct
     4381 gtcgtcccag gaggtgccgg tggtgtcgaa ggcgtccttc cagccctgga tgaatcgctt
     4441 catcgcatcg acgaactccg gcttgttgaa ggcgacggtc ttgccgtcct ccatcacctc
     4501 catggcgccg aaggaccaca tgtagggata ggctaagccc ggagggtcgc cggtgctatg
     4561 gccgagcgcc tggccgatcg gcttgcccat cgccttcagc ttcttgccca cggcgaacag
     4621 ttcgtcccag gtgtcgggga acttgtcggc accgacttgc ttcagatagc tgatgcggta
     4681 gttcagcgct gcgttggtgg tgccgatggg gacggaccac cactttccct tggccttggt
     4741 ggacttggta acccagtcgt agaagccttt gtcgcgcgtt tcgatctggg tgacgaagtc
     4801 ggtcatgtcg accagattgt cggcgtagag gtaggaccag ccgggccaga agtcgaagat
     4861 gtcggtgccg ccgcctgcct ggatggccgc ggcgatcttc gcctgcagat ccggccaact
     4921 gaggaagtcg gcactgacgg tgacaccgtt cgccttgccc cactcattca tctgatcgac
     4981 gaacagcttc tgggcagcgg ggacgaagta ggtgccgccg aggaaggcga gtgatgtccc
     5041 cttgaggact gcaggcgcgg ctgcgggtgc cgtcgtggcc gcgggagccg cgggtgcagc
     5101 accgctggcg gcgggcttcg tcggctctgc agccttcggc gcggccgtgg gtgcagcggc
     5161 aggcgcgccc gcgcaggcag ccgccgcgat ggaggcagca ccggcgccca gcgccagcaa
     5221 catttgccgc cgggtcagac gcttcttgct gattccgtct ttctcgaact ccatgcctat
     5281 cctctcctcg tgtccactct tgcctgtggc agaagtggaa gcagtgatca cctagtcact
     5341 cagacacgga cgcctttgtc cgataaccga agaacgccga catgtgcatt gggtggctga
     5401 tgtgtgaatg gagaggtggc gaaatcacag gcggtcctct gccggccaag gaggtcgacg
     5461 ccccgcctgt ctctttgagg ttacgtggca accgcactgt gtctctccct ggcagtccgg
     5521 agttggatac ccgagcagat accaaggacg ctgagaaccg ggtagtctct agaagcagtg
     5581 cctgcgagac agcgttgtgg cagttcttct aagcggtgcc atctgcgtgg atgatacagg
     5641 tgagtctcgg aaaacgcaag tacgatgttg gttttcgcat ctggtcagat tggtgaatgt
     5701 ctggtaggct ttcgtcgacg gccatcaact ttatacttgt gactcccctt ctggtggttt
     5761 ctcactcctt cccattggtg gcgatcactt gctgggcgca tcctctgcat cgtgaggtga
     5821 gtcgggtgat catcaagaac tgggacgagc tgacctccca tggtgaccgg agggggcggg
     5881 agactgccct ggcgatcctt gaggcgggcc tgcaggccgc ggatccctac gccaacgtga
     5941 agaaacttgt tcgcctggac ggcgatacgc ttcgcgtcgg ccaatcggag atggacgttt
     6001 ccggtttcgg ggaagaggtc atc
//
LOCUS       DATALS010000504         9457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_122932, whole genome shotgun sequence.
ACCESSION   DATALS010000504 DATALS010000000
VERSION     DATALS010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122932"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VHS06_11245"
     CDS             <1..641
                     /locus_tag="VHS06_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2988731.1"
                     /translation="SVRLRRPLEPEDFQVKAPEGRQRVHATVLTPRYHGSDLDSLAED
                     LEVRDGLVQVAPSRGICKFAIVERHLATGNVGVSFWRLGFQRGAVAGSIAHDHHNIWV
                     AGADDADMAAAVNRVAEMEGGFAVVENGRVLAEMALPIAGLMTDRPLATVVREIRDVE
                     QATRTLSPMEKLGPDASMQLTYITLTCYPWVYNLTDQGLLDLRNGEKLPVVW"
     gene            complement(746..2014)
                     /locus_tag="VHS06_11250"
     CDS             complement(746..2014)
                     /locus_tag="VHS06_11250"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2988732.1"
                     /translation="MSLLDAVFIGIGSTVGAGIFALFGQAGTIAGTAVWVSFLIGGII
                     ALFQAYSFAKFGSRFPSSGGQVSWIILSFGDGLFTGGTVVLLYFTMLTMSALVASSFG
                     SYAAGLMFGSQAPQIWVNIFASAIIILLTIVNIIGAQAVSKVQAVIVNVVLVVLVAFA
                     IAMLFNIDISLLSPASYPPLGSIVASVALTYFAYLGFGTIAFTGGDLEDPSRNMPRAM
                     YITVCFVALLYIALSLGVFGILPVEEVIAQANTALAAAAAPIFGDAGYTLISVAALFA
                     TAGAVNTQLYSSIGATFTMAKEGFLPPRFSEERKRGGTQGLIITAVVSLVLVNLLNIS
                     AIASLSSSVALGGYVLITVGHLRLTEQTHASKLVLYLALAVTLATLIIYLIYTLQTDP
                     RAFFVLIAFIVLAWVVEVIWRRISHRRLRS"
     gene            complement(2199..2594)
                     /locus_tag="VHS06_11255"
     CDS             complement(2199..2594)
                     /locus_tag="VHS06_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX2988733.1"
                     /translation="MSNSSDRIAIKRAYEEPGPDDGFRVLIDRLWPRGMKKETLSLDG
                     WMKEIAPSDELRRWFGHDPAKWEEFRRRYREELSSPERSRLANGLVHYAEQGKVTLVY
                     GAKDEEHNDAVVLRELLLQRLSRPQRRAA"
     gene            2888..3502
                     /locus_tag="VHS06_11260"
     CDS             2888..3502
                     /locus_tag="VHS06_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2988734.1"
                     /translation="MKAIDPRCEEQLRRELAANLDGSFARLVLTFQDRLFAFAYRVSG
                     SRPDAEEIVQDSFVRAYRALQGYSAERVQQLALRSWLYQITLNLCRNRLRSRHYREIP
                     LDREIDGEAIDPPDDEADRPDALAEQTEQRGEIAGLVAALPERYRVAVLLRFMEELSY
                     ADIAAILDLPVGTAKSNVHRGIEMLRAALSEREMARPVGVEERQ"
     gene            3499..4299
                     /locus_tag="VHS06_11265"
     CDS             3499..4299
                     /locus_tag="VHS06_11265"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX2988735.1"
                     /translation="MNDMDRLSVMSDRELLRELRGLGDLRAPSFLPGSILAEVGLADG
                     YWHMETQFGRIYLAYNAGGISAVMPAADDESFERAFRARFQRTPMAVQKPPAKLARRV
                     AEQLAGEGRQPLSYDLRGLTEFERAVLLKAMEIPRGEVRPYAWIAREIGHPGAVRAVG
                     TALGNNPVPLLIPCHRVVRSDGAIGNYAFGGEMKRAVLREEGVEIEELERLVREGVRY
                     FGSDTTHIYCYPTCRHARRVNPSHLVTFGSAGEASSAGYRPCHVCRPA"
     gene            complement(4377..6011)
                     /gene="groL"
                     /locus_tag="VHS06_11270"
     CDS             complement(4377..6011)
                     /gene="groL"
                     /locus_tag="VHS06_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258594.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX2988736.1"
                     /translation="MAKQLKFDEEARYALKRGIDALADAVKITLGPKGRNVVLDKKFG
                     APTITNDGVTIAKEIELEDPFENMGAQMAKEAATKTNDVAGDGTTTATVLAQAIVAEG
                     LKNVTAGANPMLLKRGIDKGVAAVVEELKAMSTPVKGQSDIAQVASISAADEEIGKLI
                     AEVMDKVGKDGVITVEESKGLRFETEYTEGMQFDRGYISPYFVSNPDRMEAVLDDPYI
                     LITDKKISAISDILPVLEKFLQVSKNLVIVAEDVEGEALATLVVNKLRGTINVLAIKA
                     PGFGDRRKAMLEDIAILTGGKVISEDVGRRLDSTTVQDLGRARRAVANKDNTTIIEGR
                     GEDAAIQARIKQIKAQIEETTSDYDREKLQERLAKLAGGVAVIKVGAATEVELKEKKH
                     RVEDALSATRAAVEEGIVPGGGVALVNAIQALDKVASEMKGTGDAETGVFILRRALEE
                     PLRRIAINAGQEGSVIVERVKNSEKGMGYDALKNEYAPMVERGIIDPLKVTRAALENA
                     ASIASMILTTESLVTDIPEKEKAPAMPPGGMGGMEY"
     gene            complement(6091..6387)
                     /gene="groES"
                     /locus_tag="VHS06_11275"
     CDS             complement(6091..6387)
                     /gene="groES"
                     /locus_tag="VHS06_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX2988737.1"
                     /translation="MAIGLKPLGDRVVVKPLSREEVTKSGIVLPDTAKEKPQEGSVVA
                     VGPGRLLDNGQRVAVELKEGDRVLFQKYAGTEFKRDDEELLILSEKDILAVVTA"
     gene            complement(7054..8046)
                     /gene="tsaD"
                     /locus_tag="VHS06_11280"
     CDS             complement(7054..8046)
                     /gene="tsaD"
                     /locus_tag="VHS06_11280"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX2988738.1"
                     /translation="MKILGIESSCDETAASVLADGRVMESNVVASQIDLHRKYGGVMP
                     EVASRAHLKAILPTVEEAMERAGVGWSDISALAVTRGPGLAGSLLVGVNAAKAISMAR
                     DIPLVGVNHLEGHIYSNWVITDQPFPEPAFPLICMVVSGGHTDLYLMTDHGVYRRLGS
                     TRDDAAGEAFDKVARILGVGYPGGPAIQKAAKGGNPNAFPFPRAKLGDSYDFSFSGLK
                     TAVLRTVQSIEGELPVADLAASFQEAVVDILTSKMARAAEEFGAAQGAICGGVSANLA
                     LREMAKERLSVPLHIPPINLCTDNAAMIASAGYYRFVAGARDGLDLDVSPNLSL"
     gene            complement(8162..>9457)
                     /locus_tag="VHS06_11285"
     CDS             complement(8162..>9457)
                     /locus_tag="VHS06_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988739.1"
                     /translation="PKQPLYPPGPPLEVVGKPSVRLKSQWPDRWSDRRRFPVPWARKL
                     ILMLVLAALFALIGNAVISGWQSWQQGNSVALAKEAEQKRTLATTAQDQAAARALLSE
                     SCDLYSRSLKGKEDESTRKAAMAAQDDLDRLDKTVRVNQTTQVVDYSTVADDKADVTQ
                     MVIDANNLYVLDEGQDRVFKYLLNADGKSIQEPGKHPVLFKRGDKLDGATVGDILSLA
                     WMPAGQLRNSPALFALESGRSMVTYDPKTGLSRLEVSDSQKWGSIQAINGFAGGLYLL
                     DTKLKGIFYYPPTKNGYESEPYTIVDSRTKVDLAKAVDIALDGNLYVLEGSGTIKRFN
                     REGRPFDFVTDLPDGQVKGPKALFASANTRSVYLLDTVGERILQMSPEGKLQRQFKAG
                     SKDVSFKDARDIFVDEAGRKLYLLARKSLTVFDMPSLAN"
BASE COUNT         1766 a         2829 c         2947 g         1915 t
ORIGIN      
        1 ggagcgtgcg gttgcgccga ccgctggagc cggaagattt ccaggtgaag gcgccggagg
       61 ggcggcagcg ggtgcacgcg accgtgctca cccctcgcta ccacggctcg gatttggact
      121 cactggccga ggatctggag gtccgggacg ggctggtgca ggtagccccg agtaggggga
      181 tctgcaagtt cgccatcgtg gaacgccact tggccacggg caacgtcggc gtttccttct
      241 ggcggctggg cttccagcgt ggggccgtgg ccggcagcat cgcgcacgat caccacaaca
      301 tctgggtcgc gggtgccgac gatgccgaca tggccgcggc ggtgaaccga gtcgcggaga
      361 tggagggtgg tttcgccgtg gtggaaaacg gtcgggttct ggccgagatg gcgctgccca
      421 tcgccggact gatgaccgac cggccgctgg cgacggtggt ccgtgagatc cgggatgtgg
      481 agcaggccac ccgcaccctc agcccgatgg agaagttggg cccggacgcc agcatgcagc
      541 ttacctacat caccctcacc tgttacccct gggtgtacaa ccttacggac caggggctgc
      601 tggaccttcg caacggtgag aagctgccgg tggtgtggta gtcggggaca gtacacaaca
      661 ggcgcccccc atgcgccctg taaagggtga ccggagggtt ctcgcaaatc atgagaggca
      721 gaatgggttt cgcatccatt ctgccttagc ttctcaggcg tcggtggctt atgcggcgcc
      781 aaatcacctc aactacccag gccagcacga tgaaggcgat caggacgaag aatgcccgtg
      841 ggtcagtctg aagtgtgtaa atcaggtaga ttatcaatgt ggcaagagtg acggctaagg
      901 ccaggtatag gaccagtttt gaggcatgcg tttgttcagt taagcgcaga tgcccgacgg
      961 tgatgagaac ataacctccc agggcaaccg agctggatag tgaagcgatt gcgctgatat
     1021 tgaggagatt gaccagtact agactgacta ctgctgtaat aatcaggcct tgagtaccac
     1081 ctctcttcct ttcctcagag aatcttggcg gcaagaaccc ctccttagcc atagtaaagg
     1141 tggcaccaat agagctatac aattgagtgt tgacggcacc cgctgtcgcg aagagggccg
     1201 caacagatat tagcgtatag cctgcgtcgc cgaagattgg tgcggctgcc gcagccagag
     1261 cggtatttgc ctgcgcaatg acttcttcga cgggcagaat accgaaaacg cccaaagaaa
     1321 gagctatata cagaagtgcc acaaaacaca cggtgatgta catagctcta ggcatgtttc
     1381 gtgaagggtc ttccaggtcg cccccggtaa aagcgatggt cccaaagccg aggtacgcaa
     1441 agtatgtcag ggcgacgctg gcaacaatgc tacctaaagg tgggtacgaa gcgggtgaaa
     1501 ggagtgatat gtcgatattg aatagcatgg caatcgcaaa ggcgaccagc acaaccagca
     1561 caacgttgac aattacggcc tgcaccttgc taacggcttg tgctccaatg atattgacta
     1621 tagtcaatag tatgatgatg gccgatgcga atatgtttac ccaaatctga ggagcttgcg
     1681 agccgaacat caaccctgcg gcatagcttc cgaaggaact ggctaccaaa gcactcatcg
     1741 tcagcatggt gaaatacagc aacacaacgg ttcctccggt gaacaaaccg tcgccaaaac
     1801 tgaggattat ccaggaaacc tgtccgccgg aagaaggaaa acgagaaccg aacttggcga
     1861 atgaatatgc ttgaaagagg gcgataatac cgccaatcag gaaggaaacc caaacagctg
     1921 tgccggcgat tgtccctgcc tggccaaaca gtgcgaagat gccggcgcca accgttgagc
     1981 caatacctat gaagacagca tcgagtaagc tcacgctctt ctcccaatcg gcaaatctcc
     2041 tcggcaccat cgtgacaagg ccacgcggcg agttgccggc cgcgtgagtt ggagatggcg
     2101 gcaagaaatg tacctgcatg gtagatccat gtgtgtgcgg gtgcgctgct aagcttggag
     2161 cgcggccgtt ctgttggagt tggggggacg gtagggtctc aggctgctcg gcgttgtggc
     2221 cggctgagcc tttgcagcag cagttcccgc agcacaacgg cgtcgttgtg ttcctcgtcc
     2281 ttggcgccgt agaccagggt cacctttcct tgttcggcat aatggactag tccatttgcc
     2341 agtctgctcc tctccggtga cgacagttcc tccctgtatc ggcgccgaaa ctcttcccat
     2401 ttggcagggt cgtgcccgaa ccagcgccga agctcgtcgc tgggggcgat ctccttcatc
     2461 cagccgtcca gactcagcgt ctccttcttc atccccctgg gccacaaccg atctatcagc
     2521 acccggaagc cgtcgtccgg tcccggctct tcgtaggcgc gctttatcgc gatcctgtcg
     2581 ctgctgttgc tcatgttctc ctcgcagtat cgtcaactct ggccccagcc aggcgcaccc
     2641 gtcgtgccag ggaactcgcg gcatttcggg agagtctgct cgatttgctc gcggcttgcc
     2701 atgccatcca tgcccctgca accaattggg tgagaagcgt gttgtaggga gtggaccgga
     2761 cactcgaggg gaagtaacct ttgaggtgaa atcgaggact ctacccgatg catggagtga
     2821 gtgtatccga ccgttgatga ggcaaggctg agtgaggatt agctgtggta ttggatcgag
     2881 gtagcccatg aaggccatag acccacggtg tgaggaacag ctgaggaggg aactggccgc
     2941 caatctggat ggctccttcg cgaggttggt gctcaccttt caggaccggc tgtttgcctt
     3001 cgcttatcgc gtttccggca gccgtcccga cgccgaggag atcgtccagg atagcttcgt
     3061 ccgtgcctac agggcactgc aaggctactc cgcggagcgg gtgcagcagc ttgccctgag
     3121 gtcctggcta taccagatca cgctgaacct gtgccgcaac agacttcgga gtcgacatta
     3181 tcgggagatc cccctggacc gagagatcga cggagaagcg atcgacccgc cggatgacga
     3241 ggcggatcga ccggatgcgc ttgccgagca gacggagcag cgcggcgaga ttgccgggct
     3301 ggtcgcggcc ctgcccgagc ggtatcgggt tgcagtgctg ctacgcttta tggaggaact
     3361 ctcctatgcc gacatagccg cgatcctcga tctccccgtg gggacggcca agtctaacgt
     3421 tcacaggggg atcgagatgc tcagggcggc gctgtcggag cgggagatgg ctcgacccgt
     3481 gggggtggag gaacggcaat gaacgatatg gatcgacttt cggtgatgtc tgatcgcgaa
     3541 ctgctgcgag agttgcgggg actgggggat ctgcgcgctc cctcttttct gccgggcagc
     3601 atcctggcag aggttggcct ggccgacggc tactggcaca tggaaaccca gtttggccgg
     3661 atctacctcg catacaatgc gggcgggatc tcggcggtca tgcctgctgc cgacgatgag
     3721 tccttcgagc gggccttcag ggcgcgcttc cagcggaccc cgatggcggt acagaagcca
     3781 cccgcgaagc tggcgcggag ggttgcggag cagttggcgg gggagggccg gcagccgctg
     3841 agctacgacc tgaggggact cacggagttc gaacgggcgg tcctgctgaa ggccatggag
     3901 ataccgcgtg gcgaggttcg accctatgcc tggattgcca gggagatcgg ccatcccggg
     3961 gcggtgcggg cggtgggaac ggctctcggg aacaaccccg ttcccctgtt gataccctgc
     4021 catcgggttg tacggagcga cggtgccata ggcaattacg ccttcggcgg ggagatgaag
     4081 agggcggttc tgagggagga gggtgtcgag atagaggagt tggagcggct ggtgagggaa
     4141 ggcgtgcgtt acttcggcag cgacactacc cacatctact gctacccaac ctgccggcat
     4201 gccaggaggg tcaaccccag ccacctggtg accttcggct cggcggggga ggcatcttcg
     4261 gcgggatacc ggccctgcca tgtttgccgc ccagcctagt gggggggagt gcagaggggg
     4321 ccgtgaggcc ccctctggcg ggatctgggg tgtccccaga ccccaattac tactatctag
     4381 tattccatcc cgcccatgcc gcctggaggc atcgccggag ccttttcctt ctccgggatg
     4441 tcggtaacca gcgactcggt ggtcaggatc atcgatgcga tggaggccgc gttctccagg
     4501 gcagccctgg tgaccttcag cggatcgatg atgccccgct cgaccatggg agcgtactcg
     4561 ttcttcaggg catcgtaacc catgcccttc tcggagttct tcacgcgctc gacgatcacg
     4621 gagccttcct ggccggcgtt gatcgcgatc cgccgcagcg gctcttccaa cgccctgcgc
     4681 aggatgaaga cgccggtctc ggcatcgccg gtccccttca tctcggaggc aaccttgtcc
     4741 agagcctgga tggcgttcac cagcgccacg ccgccgccgg gcacgatgcc ctcttccacg
     4801 gccgccctgg tcgcggacag cgcatcctcg acccggtgct tcttctcttt cagctcgacc
     4861 tcggtggccg caccgacctt gattaccgcg acgccgccgg ccagcttcgc cagccgctcc
     4921 tgcagcttct cgcggtcgta gtcggaggtg gtctcctcga tctgcgcctt gatctgcttg
     4981 atcctggcct ggatcgcagc atcctcgccc cggccctcga tgatggtggt gttgtccttg
     5041 ttagcaacgg ctcggcgagc cctaccaaga tcctggacgg tggtggagtc cagcctgcgg
     5101 ccgacgtcct cggagatgac cttgccgccg gtcaggatcg cgatgtcctc cagcatcgcc
     5161 ttgcggcgat cgccgaagcc cggcgccttg atggccagaa cgttgatggt gccgcgcagc
     5221 ttgttgacca ccagggtggc cagtgcctcg ccctcgacgt cctcagcaac gatcacaagg
     5281 ttcttgctga cctggaggaa cttctccagc acgggcagga tgtcggagat ggcggagatc
     5341 ttcttgtcgg tgatcaggat gtaggggtcg tccagaacgg cttccattcg atcggggttg
     5401 ctgacgaagt agggggagat gtagccgcga tcgaactgca tgccttcggt gtactcggtc
     5461 tcgaagcgga gacccttgga ctcctcgacg gtgatgacgc cgtccttgcc gaccttgtcc
     5521 attacctcgg cgatcagctt gccgatctcc tcgtctgccg cggagatgga ggcaacttgg
     5581 gcgatgtcac tctgcccctt gaccggggtg gacatggcct tcaactcttc gacaacggca
     5641 gcaacgccct tgtcgatgcc gcgcttcagc agcatcgggt tggcaccggc ggtaacgttc
     5701 ttcaaaccct cggccacgat cgcctgggcc agcacagttg cggtggtggt gccgtcaccg
     5761 gcaacatcgt tggtcttggt ggccgcttcc ttggccatct gggcgcccat gttctcgaag
     5821 ggatcctcaa gctcgatctc cttcgcgatg gtgacgccgt cattggtgat ggtgggggcg
     5881 ccgaacttct tgtccaggac gacgttgcgt cccttgggcc ccagggttat cttgacggcg
     5941 tcggccaggg catcgatccc tcgcttgagg gcgtacctgg cttcctcgtc aaacttcagt
     6001 tgctttgcca tctagttctt cctcctgaag ggccgaagcc ttcggtgtta ggtgtacatg
     6061 gtgcccggtc gagtgcgggc ctggacgcaa ttaggccgtg acgacggcca gaatgtcctt
     6121 ctcgctgagg atcagcagct cttcatcatc ccgcttgaac tcggtaccgg cgtacttctg
     6181 gaagaggacc ctatcgccct ccttcaactc aaccgcaacc ctctggccgt tgtccagcag
     6241 tcgaccgggg ccgacagcca ctacgctgcc ctcctggggc ttctccttgg ccgtatcggg
     6301 gagtacgatg ccgctcttgg taacctcctc ccgggacagg ggcttcacga caactcggtc
     6361 accaaggggc ttgagtccga tagccatgtc tcttaaacct cctcactatg agatctgcat
     6421 ccgacgattg atgtccgttt cttacggttt agcactctcg cggatagagt gctaaagaca
     6481 agcggtatat taagcccagt ggcccctgag tcgcaactac acgtttcgac ggtccagagt
     6541 atgctgcata ccggagagcc acaatagtct atcaatctcg cgattgctcg atcaagctcg
     6601 atgaatcgcg agcaaatgct ggcggtggcc gatgaggggc ttggaccgca cctgtagcag
     6661 caaatcctgt gcccactttt cctgtagaag tagcagtccg ccgcggggaa tgccagggta
     6721 ggcggtaggg gatgggggat ggggttgtgg gacgcgggaa cgcggagaca cggggacacg
     6781 gagagagtcc ctgggagcgc gaagcgaagt gcccggtacg gttcagctat gggaagaggg
     6841 attccctggt gaaggcgtag ggcggggtct tgtgccccgc ctagagcgtg gcgaaaatcg
     6901 gcatgattgg gcgtggtcac gacgtggaag gcggggcaca agaccccgcc ctacgatatg
     6961 atcgagcttt gcctctgcac caggccgaac cattttgggc atattgtcgg tatggtcacg
     7021 tttggttcgt caacggcact ccgaggcttg tggctacagg gacaggttgg gggagacgtc
     7081 cagatccaac ccgtcccggg cgcccgccac gaaccggtag tagccggcac tggcgatcat
     7141 cgctgcgttg tccgtgcaga ggttgatcgg aggtatgtgc agcggcacgc tcagccgctc
     7201 tttcgccatc tccctcagtg cgaggttggc cgatactcct ccgcagatgg ccccctgtgc
     7261 cgccccgaac tcctctgccg ctcgcgccat cttcgacgtg aggatgtcga ctacggcctc
     7321 ctggaagctg gcggccaggt ctgcaaccgg caactccccc tcaatcgact gtacggttcg
     7381 cagcaccgcc gtcttcagtc cgctaaagct gaagtcgtag ctatccccca gcttcgcccg
     7441 agggaagggg aatgcgttgg ggttgccgcc cttggccgct ttctgtatgg ccggacctcc
     7501 cgggtacccg acccccagaa tgcgggcgac cttgtcgaat gcctcgcccg cggcgtcgtc
     7561 cctggtgcta cccagccgcc gatagacccc gtgatcggtc atcaggtaaa ggtcggtgtg
     7621 gcccccagag acgaccatgc agatcaacgg gaaggccggc tcagggaacg gttgatcggt
     7681 aatgacccag ttggagtata tgtggccctc caggtggttc actcccacca gtgggatatc
     7741 tctcgccatg gaaatcgcct tggcggcgtt tacgccgact agaagcgatc ctgccagccc
     7801 cggcccgcga gttacggcca gcgctgagat gtcgctccat ccaaccccgg cgcgctccat
     7861 cgcttcctcc acggtgggga ggatcgcctt cagatgggcg cgcgaagcca cctccggcat
     7921 cacccctcca tacttgcggt ggaggtcgat ttgggaggcg accacgttgg attccattac
     7981 ccggccgtcg gcgagcaccg acgccgccgt ctcatcacag gaggattcta tgcccaggat
     8041 cttcatactt cacatcacgt aaaaaaagta ggaggtctgg ggcgatcccc aaacctcact
     8101 ctctggctcg tcaacggggt agggaaaccc catcccccac cccctatccc ccaaccccca
     8161 atcagttggc gagggacggc atatcgaata cggtgagcga tttgcgtgcc agaagataca
     8221 actttcggcc cgcctcgtcc acgaagatat cgcgggcgtc cttgaaggac acgtccttgc
     8281 tcccggcctt gaactgccgc tgcagcttgc cttccgggga catctggagg atccgctcgc
     8341 cgactgtgtc cagcagatat acagaccggg tgttggcgct ggcgaagagc gccttgggtc
     8401 cctttacctg cccgtccggc agatcggtca cgaagtcgaa gggacgccct tccctgttga
     8461 accgcttgat ggttccactc ccttccagca cgtacaggtt gccgtccagc gcaatatcca
     8521 ctgctttggc gaggtccacc ttcgtcctgg agtccacgat ggtgtagggc tcactctcgt
     8581 aaccgttctt ggtcggggga tagtaaaaga tcccctttag cttcgtgtcg agcaggtaga
     8641 ggcccccggc aaagccgttg atggcctgga tacttcccca cttctggcta tccgagacct
     8701 ccagacgaga cagcccggtc ttgggatcgt atgtgaccat ggagcgcccg ctctccaagg
     8761 cgaagagcgc gggcgagtta cgcagttgac cggcgggcat ccacgccagg gagaggatat
     8821 cgccgacggt ggcgccgtcc agtttgtcgc cccgcttgaa cagcacgggg tgcttgcccg
     8881 gttcctggat gctcttcccg tcggcgttca gcagatactt gaataccctg tcctgtccct
     8941 cgtccagcac gtacaggttg ttcgcgtcta tcaccatctg agtgacgtct gccttatcgt
     9001 cggccacggt ggagtagtcc accacctgag tcgtctggtt gacccgcacg gtcttgtcca
     9061 gccgatccag gtcgtcctgg gcagccatgg ccgccttgcg ggtgctctcg tcctccttcc
     9121 ccttcaggga tcgactgtag aggtcgcacg attcggacag cagtgctcgc gcggctgctt
     9181 ggtcctgcgc ggtagtggcc agcgtgcgct tctgctcggc ctcctttgcg agggcgacag
     9241 agttgccctg ctgccacgat tgccaaccgc tgatgacggc gttgccgatc agggcgaaga
     9301 gcgcggccag aacgagcatg aggatcagct tgcgagccca gggcaccgga aaacgcctcc
     9361 ggtcgctcca tctgtcgggc cattgcgact tcagccgaac cgacggtttg ccgacgacct
     9421 ccagcggtgg tccgggtgga tagaggggct gtttcgg
//
LOCUS       DATALS010000505         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_357351, whole genome shotgun sequence.
ACCESSION   DATALS010000505 DATALS010000000
VERSION     DATALS010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357351"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VHS06_11290"
     CDS             <1..1341
                     /locus_tag="VHS06_11290"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX2988740.1"
                     /translation="HAPAGWLQIALWLAILPKGAYTFGMAVASGRVLMLILHLASVFL
                     LFKVTRAFSGSLLAATAATLMFTLSPLSLYYQRMVLLDNIMVFWVLLSIYVVQYHGSR
                     LMTVLGSGAAMGIAILTKENAVFFLPVVGYLLFRTVRAGHMRRFAAWGWVFAVVMVVS
                     VYPLYALLKTELLPADMGFLSDGAPAQHVSLIGTIGWQLQRSGGGILDPYSQFWRFFW
                     AKWWAKDSVIIVVGMSAAIINLLAGYLLRRVNFVLVSLLCLLFGVYLARGSVMLEFYV
                     VPILPFLAMNFGLLVGLVMDRWPALLGVPLLLAADICMIGLFVYTARDHYLIDFTRLQ
                     KEQLAWVQENISPSSVMVVDDDIWVDLHEPDGDTPTFPRAHSHWKVAQDPAVQVQALH
                     NDWRSVDYLIMSNKLLTTFNEGSETLPIEIYDHSRLVAAFADGDVQLQVRQVVK"
     gene            1354..>2480
                     /locus_tag="VHS06_11295"
     CDS             1354..>2480
                     /locus_tag="VHS06_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988741.1"
                     /translation="MSIRRIAPVVLAALVLVVVGFRLFGGGNTASLISLEGQSGSSSQ
                     AQGNRSQGQGQQSNKPQATPTAGAATAEGLDQASVQKDAFILLNPASATAGSNIGVSG
                     SGFDPASVIDFYLTSDPKNDKKPQELGFAQADSGGSFGNFGFGLPPNYRNASFTVIAK
                     ERNSDKTAQATGRLESLRPSVKLGTTVGTVGDNVQISAQGFMPGEEVKVYFNKLNSDA
                     FQSFKASDGGTIEKQAVKIPYGPVGNNSLIFVGTQSQAPVTVQFLMLTLYPNVAMSSY
                     AAKADTVLSFSGSGFGPDEDVDVHLNNPQAMPIVKFHSGGDGTFENSGAFVIPFELTG
                     KNTLIFIGEKSQATATAGFDVLPYTPNVQPSTYGGRPGTAIT"
BASE COUNT          436 a          735 c          775 g          534 t
ORIGIN      
        1 cacgcccctg ccggctggtt acagattgcc ctctggctgg cgatccttcc caagggggcg
       61 tataccttcg gcatggccgt cgccagcggc cgggtgctga tgctgatcct gcaccttgcc
      121 tcggtgttcc tgttgttcaa ggttaccaga gccttctccg gcagcctcct tgctgctacc
      181 gccgcgacgt tgatgttcac cctgtcgccg ctctccctct actaccagcg gatggtgctg
      241 ctggacaaca tcatggtgtt ctgggttcta ctctcgatct acgtggtgca gtaccacggt
      301 tctcggttga tgaccgttct ggggagcgga gcggcgatgg ggatcgcgat cctcaccaag
      361 gagaacgccg tcttcttcct gcctgtggtg ggctacctgt tgttccgtac tgtccgggct
      421 ggccacatgc gccgcttcgc ggcctggggc tgggtgtttg ccgtggtgat ggtggtttcg
      481 gtctacccgc tgtacgcgct gctgaagacg gagcttcttc cagcggacat gggtttccta
      541 tcggacggcg cgccagcgca gcacgtgtcg ttgatcggca ctatcggctg gcagttgcag
      601 cgttccggag gcggaatctt ggacccgtac tcccagttct ggcgtttctt ctgggcgaag
      661 tggtgggcca aagactccgt gatcatcgtg gttggaatga gtgcggccat catcaacttg
      721 ctcgccgggt acctgcttcg gcgggtcaat ttcgtcctgg tttcactgct gtgcctgctg
      781 ttcggggtgt atctggctag gggcagcgtg atgctggagt tctatgtcgt gcctatcctg
      841 cccttccttg ccatgaactt tggactgctg gtggggctgg tgatggaccg gtggcctgcg
      901 ctgctggggg tgccgctgct gcttgcggcc gatatctgca tgatcggatt gttcgtgtat
      961 acagctcgcg accactacct gatcgacttc acccgacttc agaaagagca gttggcctgg
     1021 gtgcaggaaa acatctcgcc atcctcggtg atggtggtgg acgacgacat ttgggtggat
     1081 ctgcacgagc ccgacggcga cacgccgacc tttcccaggg ctcactccca ctggaaggtc
     1141 gcccaggacc ctgcggtaca ggtccaggct ttacataacg actggagatc agtggactac
     1201 ctgatcatgt caaacaaact tctgaccacg tttaacgagg gtagtgagac cctgccaatc
     1261 gagatctacg atcactctcg cctggtggct gccttcgccg acggcgacgt gcagttgcag
     1321 gtccgccagg tggtcaagta gggaggtatg aagatgtcca taaggaggat cgcccctgtc
     1381 gtactggccg ccttggtgct ggtcgtcgtg gggttccgcc tgtttggagg ggggaacacc
     1441 gcttccctca tttccctgga ggggcaatcg ggctcctcat ctcaggcaca gggtaaccgc
     1501 agccaggggc agggacagca gagcaacaag cctcaagcca cccctacggc tggggcagct
     1561 accgcggaag gacttgacca ggcgagcgtc cagaaggatg ccttcatcct gctgaacccg
     1621 gcgagtgcca ccgcgggctc caacatcggc gtcagcggct cgggcttcga cccggcctcg
     1681 gtcatcgact tctatcttac gtccgatccg aagaacgaca agaagcctca ggagcttggc
     1741 tttgcccagg ctgactcggg gggcagcttt ggcaacttcg gcttcgggct gccgcctaac
     1801 tatcgcaacg cctcctttac cgttatcgcc aaggagcgga acagcgacaa gacggcgcag
     1861 gccacgggca ggttggagag tctgcgcccc agtgtgaagc tggggaccac cgtgggcacg
     1921 gtaggggaca acgtgcagat ctccgcgcag ggctttatgc cgggcgagga ggtgaaggtc
     1981 tacttcaaca aactcaacag cgatgcgttc cagagtttca aggcgagcga cggtgggacc
     2041 atcgagaagc aagcggtcaa gatcccctac ggcccggtgg gcaacaactc cctcatcttc
     2101 gtggggaccc agagtcaggc gccggtcacg gtgcagtttc tgatgctgac cctctacccc
     2161 aacgtggcga tgagcagcta cgccgcaaag gcggatacgg tgctctcctt ctctggatcc
     2221 ggcttcggtc cagatgagga tgtggacgtg cacctgaaca acccgcaggc gatgcccatc
     2281 gtgaagttcc actccggagg tgacgggacg ttcgagaact cgggagcctt cgtcatcccc
     2341 ttcgaactga cggggaagaa cacgctaatc ttcatcgggg agaagagcca ggcgaccgct
     2401 acggcgggct tcgacgttct gccctataca ccgaacgtgc agccatccac ttacggtggc
     2461 cggcctggta ctgccattac
//
LOCUS       DATALS010000506         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_357661, whole genome shotgun sequence.
ACCESSION   DATALS010000506 DATALS010000000
VERSION     DATALS010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357661"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VHS06_11300"
     CDS             complement(<1..294)
                     /locus_tag="VHS06_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988742.1"
                     /translation="MIRSFRHGWRVAALLWPVVLLSYLVDTALAALVAVPPGAQLSGV
                     FGRSTMAPALLGPVSLDWLVEAFSGDGVGYFPWLLYMLVPLLYLLVGTFLRGGL"
     gene            complement(330..2237)
                     /locus_tag="VHS06_11305"
     CDS             complement(330..2237)
                     /locus_tag="VHS06_11305"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX2988743.1"
                     /translation="MHRVSAVLAFGPRQRKSALRVAAPLRPAVGLSLLVLILLFALTP
                     ASSAHAQAKEIASYQISVKLDTEQKLLSGSERIGFLNDTANPVGDLYLHLYPNAFRGI
                     DSTFMRENPEEARRAVAENNWGYMDLSVLRLASGQDLLAAATLDDTVLHVPLPAPLSP
                     GETLQMETQFTVKLPRLIARMGYQGDQYTIAQWFPKLAVLTDSGWNAHQYHANSEFFA
                     DFGSYRVEITLPDRFVVGATGLPAGERENGDGTKALTFQANSVHDFAWVANPQFREAW
                     GRAGSTDIRLLYPPEHDGVKQRFLDAAVAALENFGRWYGPYPYARLTVADVPERAGAG
                     MEYPTLVTVSQEVPSSLGVLDIEQVTIHEIGHQWWYGMVATNEFEEAWMDEGFTTYST
                     RKLSDQLYGADRSMGDVLGLRIGQLAADRGQYLAVSSLDPPDQNAWDFYSSGSYAGNV
                     YAKVDLILTTLERYLGSDRMADLLRLYFQRYAFDHPRTEDFLSVVREVAGTGYDRFFQ
                     QALYSSALFDYSVEPPIVTRDGDGFKSSVTVRREEDGVMPVEVVTTFQDGHQEREWWD
                     GEARWHRREYQSSSPVVAVEVDPEHRLALDTRWANNSYAASFQTEPILRRAADLLWLM
                     QGWLWVLGAGF"
     gene            complement(2335..3012)
                     /locus_tag="VHS06_11310"
     CDS             complement(2335..3012)
                     /locus_tag="VHS06_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX2988744.1"
                     /translation="MRSSPWFTGIVAAFVTTLIVSNIVAVKLADFGGLVLPAAVVIFP
                     VSYLFGDVLTEVYGYAQARRVIWLGFGCNLMAVIAIWVSQNLPPAAFWAPNQPAFEAI
                     LGFTPRLLLASLAAYLVGEFANAAVLSRMKLLTKGRFLWTRTIGSTLIGQGLDSAVFI
                     TVAFYGVIPLAILPVTIVTQWLFKTAYEVAATPLTYLIVNWLKRVEGMDVFDRETPLN
                     PFKIAES"
     gene            complement(3023..3391)
                     /gene="queF"
                     /locus_tag="VHS06_11315"
     CDS             complement(3023..3391)
                     /gene="queF"
                     /locus_tag="VHS06_11315"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX2988745.1"
                     /translation="MPSKPSKELTAIPNPHPDRDYDVEFSTDEFTCVCPMTAQPDFAT
                     IKISFVPDQHLVELKSLKLYLWSFRDQGIFHEDVTNVILNDLVEAIRPRRMTVVGEFN
                     IRGGIGTVVRASYPFKLNEQ"
BASE COUNT          692 a         1104 c         1113 g          581 t
ORIGIN      
        1 aaggccgccc cgaaggaacg taccgaccag caggtagagc agtggcacca gcatgtagag
       61 cagccagggg aagtagccga cgccgtcccc gctgaaggct tccacgagcc agtccaggct
      121 caccggaccc agcagggccg gggccatggt ggagcggccg aagaccccgg atagctgtgc
      181 tcccggggga acggcgacca gtgcagccag cgcggtatcc acaagataag atagcagcac
      241 taccggccac aaaagagccg ccactctcca cccatgcctg aagctacgga tcaccgacat
      301 cgctcccctc agaactcaga actcagaact cagaacccag cacccagaac ccacagccat
      361 ccctgcatca gccagagcag gtctgcggcg cgacgcagga tcggctccgt ctggaacgac
      421 gccgcatagc tgttattggc ccagcgggtg tccagcgcca gccggtgttc cgggtctacc
      481 tccaccgcca ccaccgggga actgctctga tactcccgtc ggtgccatcg agcctcgccg
      541 tcccaccact cccgttcctg gtggccgtcc tggaaggtgg tcaccacctc caccggcatc
      601 accccgtcct cctctcgccg caccgtcacg ctgctcttga agccgtcgcc atcccgagtc
      661 acgatgggcg gctccaccga gtaatcgaag agcgcggagg agtaaagcgc ctgctgaaag
      721 aagcgatcgt agccggtgcc ggcgacctcc cgtaccacgc tcaggaagtc ttcagtgcgg
      781 gggtggtcga aggcatatcg ctggaagtag agtcgcagca ggtcggccat ccggtcggaa
      841 cccaggtagc gctccagtgt ggtgaggatc agatccacct tggcgtagac gttgcccgcg
      901 taggaaccgg agctgtagaa gtcccaggcg ttctggtcgg gagggtccag actgctcacc
      961 gcgagatact gtccgcgatc ggcggcaagc tggccgatgc gcaggcctag aacgtcgccc
     1021 atagagcggt ccgcaccgta cagctgatcg gagagcttcc gggtggaata ggtggtgaag
     1081 ccctcatcca tccatgcctc ctcgaactcg ttggtggcaa ccatcccata ccaccactga
     1141 tgcccgatct catggatcgt gacctgctcg atatccagaa cacccaggga cgaggggacc
     1201 tcttgggaga cggtaaccag cgtgggatac tccattccgg cgcctgcccg ctcaggaacg
     1261 tcggcaacgg tcaggcgggc gtaggggtag ggtccgtacc agcggccgaa gttctccagc
     1321 gcggccaccg cggcatcgag gaaccgctgc ttgaccccgt cgtgctccgg cgggtagagg
     1381 agccggatgt cggtgctgcc cgccctgccc cacgcctctc ggaactgtgg attggccacc
     1441 caggcaaagt cgtggacgct gttggcctgg aaggtgaggg ccttggtgcc gtcgccattc
     1501 tccctctcgc ccgccggcag accggtggcc cccaccacga agcgatcggg cagcgtgatc
     1561 tcaacccggt aggagccgaa gtccgcgaag aactcagagt tggcatgata ctggtgggcg
     1621 ttccagccgc tatcggtgag caccgccagc ttggggaacc actgggcgat tgtgtactga
     1681 tcgccctggt agcccatcct cgcgatcagc cggggcaact taaccgtgaa ctgcgtctcc
     1741 atctggaggg tctcaccggg cgacagcggc gccggcagcg gcacgtggag cacggtgtcg
     1801 tcgagcgtcg ccgccgccag gaggtcctgg ccggaggcga gccgaaggac ggagaggtcc
     1861 atgtaccccc agttgttctc cgcgacggcg cgccgcgcct cctctgggtt ctccctcata
     1921 aaggtgctat cgatgccgcg aaatgcgttg ggatacaggt ggaggtagag atcgcccacc
     1981 gggttggccg tgtcgttgag gaagcctatg cgttcgcttc ccgagagcag cttctgctcg
     2041 gtgtccagct tcaccgagat ctgataggag gcgatctcct tcgcctgggc gtgggcgctg
     2101 gaggcaggcg tgagcgcaaa cagcaagatg aggacgagga gggataggcc gaccgccggc
     2161 cgcaacgggg ctgcgactcg cagggcagac ttgcgctgcc gagggccaaa ggccaggacc
     2221 gcggatacgc gatgcaccca gagcctccga tccgattgtg gcgatgcgcc tacctctcag
     2281 acgaacgggc cgggtcaggg acgacccggc cctcctggac tctcatccaa cgcctcagct
     2341 ttcggcgatc ttgaaggggt tcagcggcgt ctcccgatcg aagacgtcca tcccctcgac
     2401 tcgcttcagc cagttcacta tgaggtaggt cagaggggta gccgccacct cataggccgt
     2461 cttgaacagc cactgggtga ctatcgtgac cggcaggatc gccagcggta tgacgccgta
     2521 gaaggccacg gtaatgaaga ccgcggagtc caacccttgc ccgatcaggg tggacccgat
     2581 cgtccgtgtc cagaggaaac gacccttggt cagcagcttc atcctggaca gaaccgccgc
     2641 gttggcgaac tcccccacca ggtaggcagc caggctcgcc agcagcagcc tgggggtgaa
     2701 ccccaggata gcctcgaaag ccggctgatt cggggcccag aaggcagccg gggggaggtt
     2761 ctgggaaacc cagatagcga tcaccgccat caggttgcag ccgaagccca gccagatcac
     2821 ccgccgggcc tgggcatacc catacacctc ggtgagcacg tctccgaaga ggtagctgac
     2881 cgggaagatc accacggcag caggcagcac gagaccgccg aaatcggcca gcttgacggc
     2941 gacgatgttg ctcacgatca acgtcgtgac gaaagccgcg actattccgg taaaccaggg
     3001 gctggatcgc accgaaatcc tcctactgct cgttcagctt gaatgggtag ctcgcccgaa
     3061 caacggtgcc gatcccgccg cggatgttga actcgcccac gacggtcatc cggcggggcc
     3121 ggatcgcctc aacgaggtcg ttgagaatga cgttggtgac gtcctcgtgg aagatcccct
     3181 gatctcggaa ggaccagagg tagagtttca ggctcttcaa ttcgacgagg tgctggtcgg
     3241 ggacgaagct gatctttatg gtggcgaagt caggttgcgc cgtcatgggg cagacacagg
     3301 tgaactcgtc ggtcgagaac tcgacgtcgt agtcacgatc cggatgcgga ttaggtattg
     3361 cggtcagttc ctttgatggc ttgctgggca tttggacctc cttgatctta ctttaccaca
     3421 aggggcccga ggggtgagat acggggatgt gagaacgtgg atactcggag cgcatgcaac
     3481 tcgccgccgg
//
LOCUS       DATALS010000507         4382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_309828, whole genome shotgun sequence.
ACCESSION   DATALS010000507 DATALS010000000
VERSION     DATALS010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309828"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VHS06_11320"
     CDS             <1..442
                     /locus_tag="VHS06_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEX2988746.1"
                     /translation="VDNGDYSCQTWVYPGGRAAGEAVVSHEPISFIGGVDPLTGIVTE
                     RGHEIEGSNIAGKVLVYPTGKGSTGASYRLYEMVYRKTAPVALVQGERDPVTTVGCIM
                     GNIPAVDGFDQDPTEAVTTGDFVEVDADTGTVTITRKLERGGAS"
     gene            439..1101
                     /locus_tag="VHS06_11325"
     CDS             439..1101
                     /locus_tag="VHS06_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016558581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX2988747.1"
                     /translation="MSATTRELVQAFMALDTPCVSDALDRLKLRGGCEGLYPIVQGKK
                     FVGPAFTVRHIPIAQARTGAGDFIDECQEGDVIVMDNGGRSYCTVWGDILTHVAVTKK
                     CAGTVIDGVCRDVDGIRELDYPMYTRGHFMVTGKDRVVMESYQRPVSICNVQVIPGDL
                     VMADGSGVLVIPRDRAEEVLEQALEIAETERRIIEAVDAGSSLKDARATFKYDALQRS
                     KE"
     gene            1323..2693
                     /locus_tag="VHS06_11330"
     CDS             1323..2693
                     /locus_tag="VHS06_11330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2988748.1"
                     /translation="MSTTKSSSTELPTARQAKKPRGGWKTRLPFYYGWLIVAGGFFSL
                     GITYTVWYSFTVFYVALLQEFGWSRAASAGVFSLFVIVVGVAGTGAGALVDRFGPKRI
                     VAGGTALLTIGLLACSQIQDLLQFYLMFGIVSAIGLSATGWVPVVTMINRWFSARLGA
                     ALGTASAGIGVGITIMVPVSQMLITQFGWRTAYIILAGVVFFGVMPITLLVMETRPED
                     LGLHRDGRAPTPESETARLAAAAARKPREVNLKWTTTAWTLGSAVHTYRYWVLAFMMM
                     LANIGTQMIMVHQVAFLVDGGLDKLLAASIAGMVGMFSIGTKAGWGWMSDRLGREVTW
                     TIGLSSTLLAITLLISTRLAPSPLVLYLYPLVFAFGYGATAPLTPAVVSDLFAGRNFG
                     VIYGSLVIANGIGSAIGAWFAGLIFDLTGSYFIAFGLGGVCSLISIACMWVVAPRLIR
                     RVPGRC"
     gene            2717..3703
                     /locus_tag="VHS06_11335"
     CDS             2717..3703
                     /locus_tag="VHS06_11335"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX2988749.1"
                     /translation="MIQGTDLQALHRDAIVLDAHTDALLDVIAGKRRLGERSSAGQFD
                     FPRAIEGGLTVELMAMFVDPLAPGNYSRQLFQFIDAFYREAADCSDAATIALRANDVL
                     RAKAEGKVAMILSLEGAEGLEGSLGALRVCHRLGLRFLGITWNRRNEAADGVGEAQSG
                     SRLTSFGVELVEECNRLGIVLDMAHLSPSCVDHVLEISEAPVVVTHGNCAALWPHRRN
                     LTDRQLEAIASKGGVVGATCVPAFSGDDELHGSLDALLDHIDHMVRVMGDDHVGFGMD
                     FDGMEDSRMAGMEDIAQLPNLTSGLAERGHSPETIRKILGQNFLRVFRQVAG"
     gene            3898..>4382
                     /locus_tag="VHS06_11340"
     CDS             3898..>4382
                     /locus_tag="VHS06_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988750.1"
                     /translation="MNRSVSSLIAETECLVSADGAHIREANGRAAAPETTRQKELIVR
                     YAGNPILTSADWPYPINVTFNPAATLLKDGTTVLLCRIEDRRGSSHLCVARSKDGLEN
                     WEIDPVPSLAPDPIAHPEEAWGIEDARITYLEEMDRYAIAYTSYCPTGPGVSLALTSD
                     FR"
BASE COUNT          828 a         1383 c         1359 g          812 t
ORIGIN      
        1 agtagacaac ggagactatt cctgccaaac atgggtatat cccggaggca gggccgctgg
       61 agaagccgtc gtatcccacg aaccgatcag cttcatcggg ggcgtcgatc ccctcacagg
      121 catcgtcact gagagggggc acgagatcga aggctcgaac atagcaggca aggtgctggt
      181 ctaccccact ggcaaggggt ccaccggcgc gtcatatcgc ctttacgaaa tggtatacag
      241 gaagaccgcc ccggtggccc tggtccaggg ggagagagac ccggtgacaa ccgtcggctg
      301 catcatgggc aacatcccag cagtcgatgg cttcgaccag gacccgacgg aggcggtgac
      361 caccggcgac ttcgtcgagg tggacgccga cactggaacg gtgaccatca cgcggaaact
      421 tgaacgggga ggagcttctt gagcgcgaca accagagagt tggttcaggc tttcatggcc
      481 ctcgacacgc cttgcgtatc cgatgctctg gacaggctga aacttcgcgg cgggtgcgag
      541 ggtctgtacc ccatcgtcca gggcaagaag tttgtgggtc ctgcctttac cgtgaggcac
      601 atccccatcg cccaggccag gacgggggcg ggggatttca tcgacgaatg ccaggagggc
      661 gacgtcatcg tcatggacaa tggcgggagg agctactgca ccgtgtgggg cgacatcctg
      721 acccacgtcg cggtgactaa gaagtgcgct gggacggtca tcgacggagt ctgccgcgac
      781 gtggacggca tccgagagct ggactacccg atgtataccc gcggccactt catggtgaca
      841 ggcaaggacc gggtcgtcat ggagtcttac cagaggccgg tctccatctg caacgtgcag
      901 gtcatcccgg gcgacctcgt catggcggac ggctcagggg tcctggtcat ccctcgagac
      961 cgggccgaag aggtattgga gcaggccctg gagatcgccg aaacggagcg gcgcatcatc
     1021 gaagcggtcg acgccgggtc ctcgctcaag gatgctcgcg ccaccttcaa gtacgacgcc
     1081 ctccagcggt caaaggagta gcagctcaga cagccccgga ccaggtccac aaagaaggta
     1141 acggcagttg gggaagcaat cttgcttccc caactgccgt tttgtacccc tcgatccacc
     1201 ggatagccga atgccggatc tgagcgcgtg gcgcggtttc ggcgcgaggt ctataatggc
     1261 cggactctcc ggggcgaacc cgccttccgg gatataccca tgtttggcag gaatagtctc
     1321 cgttgtctac tactaagtcc tcttccacag aactcccaac cgccaggcaa gccaagaagc
     1381 cccgcggcgg ctggaaaacg aggctgccct tctattacgg atggctgata gtggccggcg
     1441 gtttcttctc gctcggtatc acctacaccg tctggtactc ctttacagtc ttctacgtgg
     1501 cccttctgca ggagttcggc tggagccgcg cggcctcggc cggggtcttc tccctgttcg
     1561 tcatcgtcgt gggagtcgcg gggacagggg cgggagccct ggtggaccga tttggaccaa
     1621 agaggatcgt ggcaggtggc actgccctgc tcaccatcgg attgctcgcc tgcagccaga
     1681 tccaggatct gctgcagttc tacctgatgt tcggcatcgt ctcggccata ggactgagcg
     1741 ccacgggctg ggttccagtc gtcaccatga tcaaccgctg gttctcggcc agactcggag
     1801 ccgccctggg aacggcatcg gcaggcatcg gggtggggat caccataatg gttcccgtca
     1861 gccagatgct gatcacccag ttcgggtggc gaaccgccta catcatcctg gcgggcgtgg
     1921 tgttcttcgg cgtgatgccg atcacactgc tggtgatgga gaccaggccc gaggacctcg
     1981 gacttcaccg ggacggccga gcgccgactc ccgagagcga gaccgcccgc ctggccgccg
     2041 ctgccgcacg caagccgcga gaggtcaacc tgaagtggac caccacggcc tggacactgg
     2101 gatcggccgt acacacctac cgctattggg tgctcgcctt catgatgatg ctggcaaaca
     2161 tcggcaccca gatgattatg gtccatcagg tggcgttcct ggtggacggg ggcctcgaca
     2221 aactgctggc ggcatccatc gcaggaatgg taggcatgtt cagcataggc accaaggctg
     2281 gctggggctg gatgtccgac aggctgggcc gcgaggttac ctggacgata gggctgagtt
     2341 ccaccctact ggcaatcaca ctgctgatct ccacgaggct ggctccgtcg cccctggttc
     2401 tgtacctgta ccccctcgtc ttcgccttcg gctacggggc aacggctcct ctgacgccgg
     2461 ccgtcgtctc cgacctcttc gccggccgca acttcggcgt gatctacggc agcctggtca
     2521 tagccaacgg catcggcagt gccatcggag cgtggttcgc tggactgatc ttcgacctta
     2581 ccggaagcta cttcatagcc ttcggcctgg ggggtgtctg ctcgctcatc agcatcgcct
     2641 gcatgtgggt cgtcgccccc cgcctgatac gtcgcgtccc cggccgctgc tagagttcgg
     2701 agtaaggagc aacaccatga tccagggtac ggacctccag gctctccatc gcgacgctat
     2761 agtgctggat gcccacaccg acgccctgct ggatgtgatc gccggcaagc ggcgtctggg
     2821 tgaacgctcc tcggcgggac agttcgactt ccccagagcc atcgagggcg gcctgacggt
     2881 cgaactgatg gccatgttcg tcgatcccct cgcccccggc aactactccc gccagctatt
     2941 ccagttcatc gacgccttct acagggaggc cgccgactgc tccgatgccg ccaccatcgc
     3001 cctgcgagcc aacgacgtgt tgagggccaa ggccgagggt aaagtggcga tgatcctctc
     3061 tctggagggt gccgaagggt tggagggcag cctgggagcg ctgcgcgtct gccaccggct
     3121 gggcctgcgc ttcctgggca tcacctggaa ccgccggaac gaggccgccg acggcgtggg
     3181 agaagcgcag agcggcagca ggctcaccag cttcggggtg gagctggtgg aggagtgcaa
     3241 ccgcctcggg atagtgttgg atatggccca cctctcgccc tcctgcgtcg accatgtgct
     3301 ggagatatcg gaggctcccg tagtagtcac ccacggcaac tgcgcggccc tctggcccca
     3361 ccgccgcaat ctgacggacc gacaactgga ggcgatcgca tcgaagggag gagtggtcgg
     3421 cgcgacgtgt gtacccgcct tctccggaga cgatgaactc cacggcagcc tggatgcgct
     3481 gctcgaccat atcgaccata tggtgcgggt gatgggcgat gaccacgttg ggtttgggat
     3541 ggacttcgac ggcatggagg acagccgcat ggccggaatg gaggacatcg cccaactacc
     3601 gaaccttacc agcggcctcg ccgagcgagg acactctccg gagacgatcc gtaagatcct
     3661 ggggcagaac ttcctacggg tatttcgcca ggtggcggga tagcctcttt tcgcgaattc
     3721 cgcccagcgc taggattcgt gctctcctgt gcgctacagc tcattcctca gcatagccca
     3781 ttagtcctat atagcttctt tatcagaatt tggtatgcta tacatcgtag gagccgggtc
     3841 cccagtgcaa ctggctcccc cacctcacct cattcccaac atccgaccgg aggatcgatg
     3901 aatcgttccg tgtcttcgct catagctgag acagagtgcc tcgtcagcgc cgacggcgca
     3961 cacatccgcg aagccaatgg ccgagcggcc gcccccgaga cgactcgcca gaaagagctc
     4021 atcgtccgct acgcgggcaa cccgatcctt acctccgccg attggcccta cccaatcaat
     4081 gtgaccttca acccggcagc gacgctgctg aaggacggga caaccgttct cctctgccgg
     4141 atcgaagata gaagaggctc atctcatctg tgtgttgcca ggtccaagga cggactggag
     4201 aactgggaga tcgaccctgt gccgagtctg gcgccggatc caatcgccca ccccgaggaa
     4261 gcctggggaa ttgaggatgc tcggataact tacctggagg agatggacag gtacgccatc
     4321 gcctacacct cctactgccc caccggcccc ggtgtctccc tcgccctgac cagcgacttc
     4381 cg
//
LOCUS       DATALS010000508         1966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_357675, whole genome shotgun sequence.
ACCESSION   DATALS010000508 DATALS010000000
VERSION     DATALS010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357675"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1176
                     /locus_tag="VHS06_11345"
     CDS             136..1176
                     /locus_tag="VHS06_11345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtaA/CmuA family methyltransferase"
                     /protein_id="HEX2988751.1"
                     /translation="MDKGTGIEERMSPKKRFLAGILGGRVDRPPVGNPTSVATVELQR
                     LTEAFFPEAHLDAEAMARLAAGGHEILGYDTVMPYFSVEQEAAALGCEVDWGSPEALP
                     VERDHPFADPSQVVIPSDLLERRPMRTVIDAISLLRKQYGSRVAIVGKVMGPWTLSYH
                     THGVQQFLLDTILEPDTVRAFLDRLKVVTLAFARAQIEAGADAICLADHATGDLVSPK
                     CYRDFLMPVHQELIRELGCPVILHICGDTTDRLEYIVEAGFDCFHFDSKVDARRAVEI
                     VGGRMSLAGNINNPQTLLLGTPEDVRREVFYALEAGVDVVAPECAVPLQTPNANLKAV
                     VDAVREYQESHA"
     gene            complement(1330..>1966)
                     /locus_tag="VHS06_11350"
     CDS             complement(1330..>1966)
                     /locus_tag="VHS06_11350"
                     /inference="COORDINATES: protein motif:HMM:NF044708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988752.1"
                     /translation="LLTKPTFLCNVTLNRDHQITGVYYGDLVAAHDKAIEFARKTAMR
                     PTPEPFDIVVTTNSGYPLDQNLYQTTKGISAATMILKRGGAVVAAAECRDGLPSHGNY
                     RDMLRMRSTPAELLEMVHQPDFHVFDQWAIQVHAMFLLKADVYLYSTIPDDEVRKAYI
                     RPIHDIEGTVRELLARRGPRATLAVLPQGPFTIPYVADESGRAYEAMSRAD"
BASE COUNT          358 a          584 c          650 g          374 t
ORIGIN      
        1 gttacctcag gggcccttca cgatcccgta tgtggcagac gagtccggcc gagcatacga
       61 ggcgatggcc agcgcagatt aactcctctt gcgaagtcag gtgaacccaa tctcatcaca
      121 ctgggggtac cctgcatgga caaaggcacc ggtatcgaag agcggatgag ccccaagaag
      181 cggtttctgg ctgggatcct gggcggcaga gtggaccgac ctcccgtcgg aaatcccacc
      241 tccgtcgcga cggtggaact gcagcgacta acagaggcgt tcttccctga ggcccacctg
      301 gatgctgagg cgatggcgag gttggccgcc ggcggacacg agatcctggg gtacgatacg
      361 gtgatgccgt acttcagcgt ggaacaggag gccgcggccc tggggtgtga ggtggactgg
      421 ggctctcccg aggcgctgcc cgtggagcga gaccatccct ttgccgaccc cagccaggtg
      481 gtgatcccct ccgacctgct ggagcgccgc cccatgcgga cagtgatcga cgccatcagc
      541 ctgctgcgca agcagtacgg cagccgcgtg gctatcgtgg gcaaggtgat gggcccgtgg
      601 acactttcct atcatacgca tggggtacag cagttcctgc tcgatacgat cctggagccc
      661 gacacggtgc gagcgttcct cgaccgactc aaggtggtca ccctcgcctt tgctcgggcg
      721 cagatcgaag caggggccga tgcgatctgc ctggcggatc acgccacggg cgacctggtc
      781 tccccgaagt gctaccggga tttcctgatg ccggtccacc aggagttgat ccgcgaattg
      841 ggctgtccgg tgatactgca catttgtggg gacacgaccg accggttgga gtacatcgtg
      901 gaggccggct tcgactgctt ccatttcgac tccaaggtgg atgcccggcg cgccgtcgag
      961 atcgtgggcg ggaggatgtc gcttgccggg aacatcaaca atccgcagac gctcttgttg
     1021 gggactccag aggacgtgcg gcgtgaggtg ttctatgcac tggaggcggg agtggatgtg
     1081 gtggcaccgg agtgtgccgt tccgttgcag acgcccaatg cgaacctgaa ggcggtggtt
     1141 gatgccgtca gggagtacca ggagagccac gcgtagtcgc acgtgtcctt ctgcctgttt
     1201 ggtgtacggt gagggaggat ggacatagct tgatctggac gtagggcggc gtcttgcgcc
     1261 ccgcccggag cgtgccatca tcacgatgct ggtcgtggcc gcggtgtggg aggcggggca
     1321 caagaggagt caatccgccc tggacatcgc ctcatatgct cggccggact cgtctgccac
     1381 atacgggatc gtgaagggcc cctgaggtaa cacggccagc gtcgcccgcg gaccacgacg
     1441 ggccaacaac tcccgcacgg tgccctctat gtcgtgaatg gggcgaatat acgccttccg
     1501 cacttcgtcg tccgggatgg tggagtacag gtagacgtcg gccttcaaga ggaacatcgc
     1561 gtgaacctgg attgcccact ggtcgaagac gtggaaatcg ggctggtgca ccatctccag
     1621 caactcggcc ggcgtcgacc tcattctcag catgtcgcga tagttgccgt gagaaggaag
     1681 accatccctg cactcggcag ccgcgaccac ggcgccgcct cgcttcagga tcatcgtggc
     1741 ggcagagatg cccttagtcg tctggtagag gttctggtcg aggggataac cgctgttggt
     1801 ggtcacgact atgtcgaacg gctcgggggt aggcctcatc gccgtcttcc gcgcaaactc
     1861 gatcgccttg tcgtgcgccg ccaccagatc cccatagtac acccccgtga tctggtgatc
     1921 tcggttcagc gtcacgttgc acagaaacgt cggtttcgtc agcaac
//
LOCUS       DATALS010000509         3645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_357680, whole genome shotgun sequence.
ACCESSION   DATALS010000509 DATALS010000000
VERSION     DATALS010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357680"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..539
                     /locus_tag="VHS06_11355"
     CDS             225..539
                     /locus_tag="VHS06_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988753.1"
                     /translation="MASLWGRLFLGIHEELAVGVVGGEAEVALLVDIHPGRGGLQETA
                     VEAGWVGVSRRGVVGCPKVGARSGGGLDGKVIHGGRSWEPSRLLIGHGGMILQEGVRA
                     EG"
     gene            734..1672
                     /locus_tag="VHS06_11360"
     CDS             734..1672
                     /locus_tag="VHS06_11360"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2988754.1"
                     /translation="MLSLFSLGVLAALLTQSDWREATRPLVEARPEWLLAAVVLALLV
                     EVAKTVRWQLLLGVGPRVLPQLLSLVFTARLLNALVPLRAGDVWRVASVVKAEGRPLL
                     RAGGSVVVEKLLDGALVGGLSLALIGKIEIGGMKAGLLAVVALAALVLFLVVCSRNGL
                     LRSFAERVGQRNSTVAGLLQLSGERWRVSDARSLLLIAEVAGLTMAGMILGLLVNLAA
                     LEAMGLPQGLMAAAVMLVSGYAVGLVPSGPAQLGVYELAVSAPLMTLGLPPASAVSAA
                     LALHLVLLVALGIGGMLALLFGYLAGRRCDAGMAGV"
     gene            1906..>3645
                     /locus_tag="VHS06_11365"
     CDS             1906..>3645
                     /locus_tag="VHS06_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988755.1"
                     /translation="MDGRSAATHCSHLLLAMLVLCLLLAQPWALVAGRYGAPRLGLHP
                     LSRQSAPAAAFDTPLVTERLLVGGASVKGMPDIDGDRVVYHQQDGSIYSVYAVSTGSG
                     SPVPLSSPAKANQMRPRVSGSSVVWSDSRLASPKPALFGYIEGADPPVSQLATRKLSI
                     SSPVAIHGTFVAYQDCTAKPYKIRLRELQSGQEIVVAASSYNQDFPSLGDDLIAFQQA
                     IDATSTTDYRVRLFSFALVGGELTTNHLVDPVASDPGSSLTNPQVIGNPADWTLVWQE
                     SEGPSPGIYGQRSGGSRFSIATGGEDQSNPAISGQTVVWQERGSTTGYDIRGYDLSRQ
                     LGFDVCAAAGDQTNPRVRGDGVVWADNRTGHSEIYLGMVQWVSPTSTPTATTASTDTP
                     TSTPTPSPSATLAPTDTAIPSATSTEVPTTPAVPTATSTATDTPTATLAPTDTPESTD
                     TPEPTVTPTPTATETAASTATSTATETVVPTDTATATATSSATPTETPTPTATATVIP
                     TATSTLVPTSTPAQTSTTVPTSTATSTPVPTPTATITPTELPTVTATPTMTATGTPIP
                     TASPTASATPQPTT"
BASE COUNT          615 a         1116 c         1199 g          715 t
ORIGIN      
        1 gcggcggcga caggcgaggg ggtggcatcc tggcttccgg acaccgtctg ccagccgaag
       61 tagagtcccc ccaaccctac cagcaggaac agccccacga ctgcccctgc cagccattgg
      121 aggataggag gcttcctcaa tccagggaag acttcagtcg aagctgagcc cgtcggcacg
      181 atgggtctat cctgggaagc ggaagacgac ctactccgtg atgagtggcc agtctgtggg
      241 gcaggctgtt ccttggtatt cacgaggagt tggcagttgg cgtggttggc ggagaggcag
      301 aagtcgcgct gctggtcgat attcaccccg gccggggggg attgcaggaa acagcggttg
      361 aggcgggttg ggtaggcgta tcgcgtcgag gggtcgtggg atgccccaag gtgggggcac
      421 gatccggcgg tggcctcgat ggtaaggtca tccatggtgg gcgtagttgg gaaccgtccc
      481 ggctcctcat cggacatgga gggatgatcc ttcaggaagg tgtgagggct gaaggatagc
      541 agtccgccat ccggtggtca actctttatc cccttgcgtg caatgtgtat cattggcggc
      601 gaagcctcct gtattttgcc gctggttacc cgatcctctc ctggctggcg accgtaggag
      661 tacaactcct ggagtggaaa tgtccgaagc tctctcgaag ctccgtcctc gtacgatacg
      721 gtgggttggg agcgtgctgt ccctgttctc gctcggggtc ctggctgctc tcctcacgca
      781 gagtgattgg cgggaggcga cccgcccgct cgtggaggcg cgaccggagt ggctccttgc
      841 tgccgtggtg ctggccctgt tggtggaggt ggcaaagacg gttcgctggc agctgctgct
      901 gggggtgggg ccgcgggtgt tgcctcagct gctgagcctg gtcttcaccg cgcgcctcct
      961 caacgccctg gtgccgcttc gtgcggggga tgtgtggcgg gtggcctcgg tggtgaaggc
     1021 ggaggggcgg ccgttgctga gggccggcgg gtcggtggtg gtggagaagc tgctggatgg
     1081 ggcgcttgtc ggcggcctga gcctggcgct gatagggaag atcgagatcg gcgggatgaa
     1141 ggcgggattg ctcgcggttg tagctctggc tgccctcgtc ctcttcctgg tggtttgctc
     1201 ccgcaatggc ctgctccgca gctttgcgga gagggtaggg cagcggaact cgacggtggc
     1261 cggactgctg cagctgtccg gagagcgatg gagggtgagt gatgcaagga gtctcttgtt
     1321 gatcgctgag gttgccgggc tgacgatggc agggatgatt cttggtctcc tggtgaacct
     1381 ggcggcgctc gaggccatgg ggctgccgca gggtttgatg gcggcggccg tcatgttggt
     1441 cagcggatac gcggtggggt tggtgccgtc ggggccggct cagttgggag tctacgagtt
     1501 ggcggtgtcg gcgcccctga tgaccctcgg cctgccgcct gcttcggcag tgtcggctgc
     1561 cctcgctctt cacctggtgt tgttggtagc gctcgggata ggtgggatgc tggcgctgct
     1621 cttcggctat ctggctggcc gtcgttgcga tgcagggatg gccggcgttt gagctaacgg
     1681 taccactgac ctttgcaccg caggatgaat cccacggcat cccgtgggat tcattctgcc
     1741 ataccgccag gggtatctca cgctatggga agcaagatac gagtcgataa tcggattctg
     1801 cagccttcgt ctcgcagtaa gggcttgaca catgtggtca gatgctggta gggttctttg
     1861 tagttgctgt tccggcggtc aggcaccata tgtggggggt gtctcttgga cgggcggtcg
     1921 gcggctaccc attgttccca tctcctgctc gccatgctgg tcctctgcct cctgttggcc
     1981 caaccgtggg cgctggtggc agggcggtat ggcgcacctc ggcttggcct ccacccactt
     2041 tccagacagt ctgctccggc tgccgcattc gacactcctc tagtcaccga gaggttgctg
     2101 gtgggtggcg ccagcgtgaa ggggatgccc gatatcgatg gcgacagggt cgtgtaccat
     2161 cagcaggatg gcagcatcta ctccgtgtat gcggtctcga ctggatcggg ttcgccggtt
     2221 ccactgtcat cgccggccaa ggcaaaccag atgaggcccc gcgtcagcgg ttcgagtgtg
     2281 gtgtggagcg actcgcggct tgccagcccc aagcccgccc tcttcggcta catcgaggga
     2341 gccgacccgc cggtgagcca gttggccacg cgcaagctgt cgatatcttc acccgtcgcc
     2401 atccacggga cgttcgtcgc ctaccaggat tgtacggcca agccgtacaa gatccggttg
     2461 agggagttac agagcggaca ggagattgtc gtcgctgcct catcttacaa ccaagacttt
     2521 ccctcccttg gtgatgacct gatcgccttc cagcaggcca tcgatgcaac cagcacgacg
     2581 gactatcgcg tgaggctgtt ctcctttgct ttggttggag gggagttgac gaccaatcac
     2641 ttggttgacc cggttgcatc ggatcccggc tcaagcttga cgaatccgca ggttatcggg
     2701 aaccccgccg actggacgtt ggtgtggcag gagagcgagg ggccatcccc tggcatatac
     2761 ggccagcgtt ctggtggatc ccgcttctcc atcgcgaccg gtggtgagga tcagagcaac
     2821 ccggcgataa gcgggcagac agtagtgtgg caagaacggg gctccacgac tggctacgac
     2881 atccgaggct acgacctgtc gagacagctg gggttcgatg tctgtgctgc tgctggggat
     2941 cagaccaatc cacgggtgcg cggcgatggg gtagtatggg cggacaatcg aactggccac
     3001 tccgagatct acctggggat ggtgcagtgg gtgtcgccga catcgacccc gaccgcaacc
     3061 acggcatcta cggacactcc aacctccact cccacgccat ctccatcggc taccctggcc
     3121 cccaccgata cggcaatccc atcggccacc tcgactgagg tgccaaccac tcctgctgtg
     3181 cccacggcta cctctacagc tactgacacc ccaacggcga cgctcgcccc aaccgacacg
     3241 ccggaatcaa ccgacacgcc ggaaccaacc gtaaccccaa caccaacggc gacggagacc
     3301 gcagcctcca cagccacctc caccgcgacg gagactgtgg tgcctactga tactgccacg
     3361 gcgaccgcga cgtcctcggc cacgcccacg gagactccga cgccgactgc cacagcgact
     3421 gtcatcccaa ctgcaacgtc cacactcgtg ccgacctcga ctccggccca gacttccacc
     3481 accgtaccga catcgaccgc cacgagcacg ccagtgccga ccccaacagc caccatcacc
     3541 ccaaccgagc tacctactgt cacagccacc ccgacgatga cagccacagg tacgccaatc
     3601 cccacagcct cgcccacggc gagcgcgacg ccccagccca caacc
//
LOCUS       DATALS010000510         6055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_75051,
            whole genome shotgun sequence.
ACCESSION   DATALS010000510 DATALS010000000
VERSION     DATALS010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75051"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VHS06_11370"
     CDS             <1..361
                     /locus_tag="VHS06_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2988756.1"
                     /translation="TGYMQREITESAYRFQRRVEQRDLTVVGVNRFAVEEKRPIQILR
                     VDDAVANKQVAKLAEVKKRRDSAAVSTRLDALRQVALDGGNVMPAIIECVEVSATLQE
                     MCDVLRGVYGEQTQTFI"
     gene            418..822
                     /locus_tag="VHS06_11375"
     CDS             418..822
                     /locus_tag="VHS06_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014684358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX2988757.1"
                     /translation="MEDRKIRVLVAKPGLDGHDRGAKVVARALRDAGMEVVYTGIRQT
                     PQTIADAALQEDVDVIGLSSLSGAHMSLFSRVMEELKRRGMDDLVVIAGGIIPDEDHE
                     ALKAMGIGAVFGPGASTQEMAEFIREAVGVHA"
     gene            819..1826
                     /gene="meaB"
                     /locus_tag="VHS06_11380"
     CDS             819..1826
                     /gene="meaB"
                     /locus_tag="VHS06_11380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX2988758.1"
                     /translation="MIIERLDEAVLSGHPRAVARAITMVETGGEDAEQLMEKLRQVPR
                     HGHLIGFTGAPGAGKSSLICKLAKEYRARGKSVGVIAVDPTSPFSGGAVLGDRIRMGE
                     LSSDPGVFVRSMASRGRPGGLAAAVEDAAVILQAAGKEIILLETVGVGQGELDVASAV
                     QTTVVVLTPGMGDEIQSLKAGILEVADVYVVNKSDLVGADTVARSLQSAAAQQDSSWV
                     RPVLLASSVTSVGVPELADAIDCHKEYLEGNRVPCEQEIRLTYQSILSIAGRQLISQL
                     QSKVDRKRLERLATEVASGEMDPREAAEEAVSLFLAAQFGAMPRQEKREAVAAGHNGR
                     N"
     gene            1863..3407
                     /locus_tag="VHS06_11385"
     CDS             1863..3407
                     /locus_tag="VHS06_11385"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX2988759.1"
                     /translation="MKGKIDELHRLRNEAKLGGGHRRIEQQHAKGKLTARERLDLLLD
                     PGSFQEIGMFVTHRCTDFGLESQKYLGDGVVTGYGTIDGRMVYVFSHDATVFGGALSE
                     AHSEKICKVMDLAMRQGAPIIGINDSGGARIQEGVVSLSAYTEIFRRNVLASGVIPQI
                     SAIAGPCAGGAVYSPAITDFIFMVRGTAHMFVTGPNVVKAVTYEDVTSETLGGADVHA
                     SKTGVAQFASDNEAECMQQLRKLMGFLPANNTEKPPYKTPTDSPDRIEERLNTIVPDD
                     PSMPYDMKEVIHLILDDGDFLEVHEDFATNLIVGFGRLNGNAVGVVAQQPASMAGVLD
                     INASVKGARFVRFCDCFNIPLITFVDVPGFMPGTMQEHGGIIRHGAKLLYAYIEATVP
                     KVSVITRKAYGGAYTVMSSKEARGDINLAWPSAEIAVMGPEGAVNIVFKDKIEKADDP
                     KAAWVAAVNEYREKLANPYVAASRGYLDDVIVPSETRPQLIKALESLKNKQDSNPPRK
                     HGNIPL"
     gene            3460..3861
                     /gene="mce"
                     /locus_tag="VHS06_11390"
     CDS             3460..3861
                     /gene="mce"
                     /locus_tag="VHS06_11390"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX2988760.1"
                     /translation="MKVLKIEHIGVAVKSSSESAAFYKLLGLEVEGEENVGSDLKVAM
                     LGVGESEIELLEPATPEATVAKFIEKRGEGIHHLALSVEDLDGAVKELLAAGVRMIDQ
                     APRPGAGGTMVAFVHPSAAKGVLIELVQAHK"
     gene            complement(4006..5001)
                     /locus_tag="VHS06_11395"
     CDS             complement(4006..5001)
                     /locus_tag="VHS06_11395"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX2988761.1"
                     /translation="MSIVHLRSRATSRLAMALGVSALTLALVGGPTGWGSQKAYAQEV
                     APSVVAVSDASTPTPIQRFLGMVDHSGATADNARGSSTQTDDGSSRAASAAPQGSDQT
                     APPATAPAPVSPPPVSAAAPASSPAASQAASPLPTAAGKHVGIQAGHWQTANLPSELA
                     ALRTSTGAAGSGWKEVDVNLDIAQRVTALLTNAGVQVDLLPSTIPVGYKADAFVALHN
                     DGNANTGLTGYKVARSAWSVITTKDDVLVKDLATDYEAGTGFTPNPQTITVNMTHYYA
                     FNNRTYQHSVAPSTPAAILEMSFLTNAKDRNEMVNDPNRVATAIARGILDFLNGK"
     gene            5283..>6055
                     /locus_tag="VHS06_11400"
     CDS             5283..>6055
                     /locus_tag="VHS06_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2988762.1"
                     /translation="MLDTLTAFSLIAVVLTVSALAAGLVDRAPLSFPMIFLAVGFIVG
                     PLGLGIVVVTPRDPYLQTLATISLAFVLFLDAVRVRFDSSSRGWTVPILDLGPGTIIT
                     LVGIALCALVIFGATPLQALLLGAILSSTDPIVLRDIVRDERLPHAVREALSIEAGTN
                     DLVVLPILFVLLAVMTGHHGSLSDWVTFLVALLVVGPLVGALVGGIGSGVMAFVDARY
                     PVRSELSPLGPNQAMNACAARLMGLPWSTTLMASGLATP"
BASE COUNT         1123 a         1842 c         2069 g         1021 t
ORIGIN      
        1 gaccggctac atgcagcgcg agatcacgga gagcgcctac cggttccagc gacgcgtgga
       61 gcagagagat ctgacagtgg tgggtgtgaa ccgcttcgcg gttgaggaga agcggcccat
      121 tcagatcctt cgcgtcgacg atgcagtggc gaacaagcag gtcgcaaagc tggccgaagt
      181 aaagaagcgt cgcgactcgg cggcggtttc gacccggctg gacgcactgc ggcaggtcgc
      241 cctcgacggc ggcaacgtga tgcccgccat catcgagtgt gtagaggtca gcgcgacgct
      301 gcaagagatg tgcgacgtcc tgcgcggagt gtacggagag cagacgcaga ccttcatcta
      361 ggggacgggc cacgagatct gcggtgaccc aacgactgta gcagaggtgt tctgatcatg
      421 gaagatagga agataagggt tctggtcgcc aagcctgggc tagacggcca cgaccgcgga
      481 gcaaaggttg tagccagggc gctgcgagat gccggcatgg aagtggtcta cacggggatc
      541 cggcagaccc cacagaccat cgccgacgcg gccctgcagg aagacgtgga tgtgatcgga
      601 ctgagcagcc tctccggcgc tcacatgtcc ctcttctcca gggtgatgga ggagctgaag
      661 aggagaggga tggacgacct ggtcgtgatt gccggcggga tcatcccgga cgaggatcac
      721 gaggcgctga aggcgatggg gatcggcgcg gtcttcggcc cgggcgcctc cacccaggag
      781 atggccgagt tcatcagaga ggcggtaggg gttcacgcat gatcatcgag aggctggatg
      841 aggcggtcct gtccggccat ccccgggcag tggcgagagc catcaccatg gtggagaccg
      901 gcggtgagga tgccgagcag ctgatggaga agctgcggca ggtgcctcgc cacggccacc
      961 tgatcgggtt cacgggcgca ccaggggccg gcaaaagctc gctgatctgc aagctggcca
     1021 aggagtaccg cgcgcgtgga aagtcggttg gcgtgatcgc cgtggatcca accagcccct
     1081 tcagcggcgg agcagtactc ggagaccgga tccgaatggg cgagctctcc tccgatccgg
     1141 gcgtcttcgt ccgaagcatg gcatcgcgcg gacgccccgg agggctcgca gcggcggtcg
     1201 aggacgcggc cgtgatcctg caggcggccg gcaaggagat catcctgctg gagaccgtcg
     1261 gcgtgggcca gggcgagctg gatgtggcga gcgcggtaca gaccacggtg gtcgtgctga
     1321 cgcccggcat gggcgacgag atccagtcgc ttaaggccgg tatcctcgaa gttgcggatg
     1381 tctacgtggt caacaagtcg gacctggtgg gcgccgacac cgtcgcccgc tccctccagt
     1441 ccgcggcggc acagcaggat agcagctggg tccggccggt gctgctggcc tcctcggtca
     1501 cctccgtggg agtgcctgag ctggcggacg ccatcgactg ccacaaggag tacctggaag
     1561 gcaaccgtgt cccctgtgag caggagatcc ggctcacata tcagtcaata ctctccattg
     1621 ccggcaggca gctgatctca cagctgcagt cgaaggtgga ccgaaaacgg ctggagcggc
     1681 tggcaactga ggtggcatcc ggcgagatgg atcctcgcga ggcggccgag gaggcggtat
     1741 ccctcttcct cgctgcccag ttcggggcca tgccccgaca ggagaagcgg gaagccgtgg
     1801 ctgccggcca caacggcagg aactagttat ttaacgagga tagaaatctc atggttgagc
     1861 agatgaaggg caagatcgat gaactgcacc ggctccgcaa tgaggcgaag ctcgggggtg
     1921 ggcatcgaag gatcgaacag cagcatgcca agggcaagtt gaccgcccgc gagcgacttg
     1981 acctcctgct cgatccggga agcttccagg aaatcgggat gttcgtcacc caccggtgca
     2041 cggacttcgg tctggagagc cagaagtacc tgggtgacgg ggtcgtcacc ggctacggaa
     2101 ccatcgacgg gcgaatggtg tacgtgttct ctcacgacgc caccgtcttc ggcggcgctc
     2161 tctcggaggc ccactccgag aagatctgca aggtgatgga ccttgcgatg cggcagggtg
     2221 cgccgatcat cgggatcaac gactcgggag gcgcacgcat ccaggagggc gttgtcagcc
     2281 tctccgccta caccgaaata ttccgtagga acgtcctcgc ctcgggcgtg atccctcaaa
     2341 tctcggccat cgccggcccc tgcgccggcg gtgcggtgta ctccccggcc ataaccgact
     2401 tcatcttcat ggtcaggggc acggcccata tgttcgtcac cgggcccaac gtggttaagg
     2461 ccgtgaccta cgaggatgtg acgtccgaga cgctgggtgg cgcggacgtg cacgccagca
     2521 agaccggcgt cgcgcagttc gcctccgaca acgaggcgga gtgcatgcag caactgcgga
     2581 agctgatggg cttcctgccc gccaacaaca ccgagaagcc gccctacaag acgcccaccg
     2641 actcgccgga tcgcatcgag gagcggctga acaccatcgt gcccgacgat ccctccatgc
     2701 cctacgacat gaaggaggtc atccacctca ttctggacga cggcgacttc ctggaggtcc
     2761 acgaggattt cgcgaccaac ctgatcgtgg gcttcgggcg gctgaacggc aacgccgtcg
     2821 gcgtcgtcgc gcagcagccc gcatccatgg ccggtgtgct ggacatcaac gcctcggtca
     2881 agggcgcgcg cttcgtccgc ttctgcgact gcttcaacat cccgctgatc accttcgtgg
     2941 acgtgcccgg cttcatgccc ggcacgatgc aggagcacgg cggcatcatc cggcacggcg
     3001 ccaagctgct ctacgcctac atcgaggcca cggtgcccaa ggtctccgtg atcacccgga
     3061 aggcgtacgg cggcgcctac accgtaatga gcagcaagga ggcccgaggc gacatcaacc
     3121 tcgcgtggcc gagcgctgag atcgcggtga tgggtcccga gggcgcggtc aacatcgtct
     3181 tcaaggacaa gatcgagaag gccgacgacc cgaaggcagc ctgggttgca gcggtcaacg
     3241 agtaccgaga gaagctggcc aacccgtacg tcgcggcgtc ccgaggctac ctggacgacg
     3301 tgatcgtgcc gagcgagacc aggccgcagc tgatcaaggc gctggagagc ctcaagaaca
     3361 agcaggactc caacccgccg aggaagcacg ggaacatccc cctgtagggg tgggggttgg
     3421 ggagctgaca cgggatacga agcagggagt ttgagagtga taaaggttct gaagatcgag
     3481 cacatcgggg tggccgtcaa gagcagctca gaatcggccg ccttctacaa actgctgggc
     3541 ctcgaggtcg agggcgaaga gaacgtcggg agcgacctca aggtagcgat gctgggcgtt
     3601 ggagagagcg agatcgagtt actggagccg gcaacccccg aggcgaccgt cgcgaagttc
     3661 atcgagaagc gcggtgaggg gatccaccat ctggcgctca gcgtcgagga cctggacggc
     3721 gccgtcaagg agctgctggc cgccggcgta aggatgatcg accaggctcc gaggcccggt
     3781 gccggcggca ccatggtcgc cttcgtgcac ccctcggcgg ccaagggcgt cctgatagag
     3841 ctggtgcagg cccacaagta aagccagaga tcgtctgtac aaagagaggg ttccggggag
     3901 cactccccgg gaccctctct ttgtactacc cggtcatcgc ggattcgatg acgtaatggc
     3961 gacgcacgaa tcgccacgac tggtcggagc cggcgtagcc gggtgctact tcccgttgag
     4021 gaagtccagt atcccgcgcg cgatcgccgt ggccacccga tttgggtcgt tcaccatttc
     4081 gttccgatcc ttggcgttgg tcaggaagct catctcgagg atcgcggccg gcgtcgaagg
     4141 cgctaccgag tgctggtagg ttcggttgtt gaatgcgtag tagtgcgtca tattgaccgt
     4201 aatggtctgc ggattcggcg tgaaccccgt accggcctcg tagtcggttg ccaggtcctt
     4261 cacgaggacg tcgtccttgg tggtgatgac gctccaggcg gagcgggcta ccttgtagcc
     4321 agtaaggccg gtgttggcgt tgccgtcgtt gtgaagcgcc acgaaggcgt ctgccttgta
     4381 gccaacggga atggtggagg gcagcaggtc gacctgcaca ccggcattgg tgagaagcgc
     4441 ggtcaccctc tgggcgatgt ccaggtttac gtcgacctcc ttccagccgg agcccgcagc
     4501 gccggtggag gtcctcagag ccgccagttc gctgggcaga ttggcggtct gccagtggcc
     4561 ggcctggatc ccgacatgct tgcccgcagc agtgggcagc ggggaggccg cctggctcgc
     4621 tgccggcgag gaagcagggg cagccgccga gaccgggggc ggggagaccg gcgcaggcgc
     4681 tgtggccggc ggcgcggtct gatcgctacc ctgcggcgcg gccgaagccg ctcgagagga
     4741 gccgtcgtcg gtttgggtcg agctccctct ggcgttgtcg gccgtcgcac cagagtgatc
     4801 gaccataccc aggaagcgct ggatgggcgt cggcgtcgac gcgtccgaca cggctacaac
     4861 ggaaggggcg acctcctgag cgtaggcctt ctgcgatccc cagcccgtcg gccctccgac
     4921 cagtgccagg gtgagggctg atacccccaa cgccatggca agcctggacg tagctctcga
     4981 gcgcagatgg acaatggaca cggcggtacc tccgatgttt cgtcgtggca atgctgtggg
     5041 aaattgctcc atccgccgtt ccggtgagcc ggaagggccg gacgggccag gggtggataa
     5101 tatacaaata gcgtactaca tatgttaact caccgtaaac agttgtctaa aggagtgttc
     5161 gtggatccgc cgatcgaacg tcggctcgct ccgcgccgac gcggcggtcg cgtgggtgga
     5221 atggggcttg cgaccgggta aactccaccg aacagtcccg tggaggagag aggaaagcca
     5281 acttgctgga tacactgacc gctttctccc tgatcgcagt ggtcctcacc gtctccgccc
     5341 tggcggcggg gctggtggac cgtgcgcccc tctccttccc tatgatattc ctggccgtcg
     5401 gcttcatcgt cgggccgctg ggactcggca tcgtcgtcgt cacgccgcga gacccctatc
     5461 tgcagaccct ggcaaccata tccctggcgt tcgtcctgtt cctggacgca gtcagagtgc
     5521 gcttcgactc ctcctccaga ggctggaccg taccgatcct ggacctcggc ccaggcacga
     5581 tcatcaccct ggtgggcatc gccctgtgtg cgctggtgat cttcggtgct acgccgctcc
     5641 aggcgctgct cctcggcgcg atcctctcgt ccacggaccc catcgtgctg cgcgacatcg
     5701 tccgcgacga gcgactcccc cacgccgtcc gtgaggcgct cagcatagag gcaggaacca
     5761 acgacctggt ggtcctcccg atcctcttcg tgctgttggc agtgatgacc ggccatcacg
     5821 gcagcctatc ggactgggta acatttctgg tggcgctgct ggtcgttggg ccgttggtgg
     5881 gcgcgctggt aggagggatc ggttcgggcg tgatggcgtt cgtcgacgcc aggtacccgg
     5941 tacgcagtga gctgagccct ctggggccga accaggcgat gaacgcctgc gccgcgcggc
     6001 tgatgggact gccatggagc accacgctga tggccagcgg cctcgctacc ccgat
//
LOCUS       DATALS010000511         4577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_358293, whole genome shotgun sequence.
ACCESSION   DATALS010000511 DATALS010000000
VERSION     DATALS010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358293"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..634)
                     /gene="tsaA"
                     /locus_tag="VHS06_11405"
     CDS             complement(137..634)
                     /gene="tsaA"
                     /locus_tag="VHS06_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX2988763.1"
                     /translation="MQNSTQPPSGMLLRPIGVIRNGYTEKPNRPWEEIESTIEVDPTL
                     EEALDGIEGFSHIIVLFWLNRVESERGKVLKQHPQRQEHLPEVGIFAIRTMFRPNPIG
                     FTAVRLLERRGPTLKVIGLDALDGTPLLDLKPYLTRGDCWPDATVPPWIEQLWAEQDA
                     GRTQT"
     gene            1013..1327
                     /gene="rplU"
                     /locus_tag="VHS06_11410"
     CDS             1013..1327
                     /gene="rplU"
                     /locus_tag="VHS06_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004834209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX2988764.1"
                     /translation="MYAVVETGGKQYRVEVGQTVDVEHIPAEVGDTVELGRVLMVSTD
                     DGVRLGSPVLEGAKVTASVVDQRRGKKIIVFRYKPKTRYRKKTGHRQDLTRVSIQGIA
                     LE"
     gene            1339..1602
                     /gene="rpmA"
                     /locus_tag="VHS06_11415"
     CDS             1339..1602
                     /gene="rpmA"
                     /locus_tag="VHS06_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX2988765.1"
                     /translation="MAHKKGVGSSRNGRDSASQRLGLKVGDGQYVHAGSILVRQRGTA
                     MRPGDNVGLGKDHTLFALISGEVKFGNYSKEQKKVSIYAAPTA"
     gene            1628..1834
                     /gene="rpmE"
                     /locus_tag="VHS06_11420"
     CDS             1628..1834
                     /gene="rpmE"
                     /locus_tag="VHS06_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX2988766.1"
                     /translation="MKQGIHPKYAETTIVCACGAQHKTSSTKSNLRIDVCSQCHPFFT
                     GEQRIMDTAGRVDKFMKKLQKKVR"
     gene            1904..2857
                     /locus_tag="VHS06_11425"
     CDS             1904..2857
                     /locus_tag="VHS06_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX2988767.1"
                     /translation="MSSSGKSFTYGGQAVLEGVMMRGSRWSTVAVRAPSSEIVLHSEH
                     LPKALYDSWFIKIPFVRGVGMLWDTLVLGMRSLMFSANVALQEEGEDGEQPAEMSKPM
                     MWGTVAVAILFAIGLFFVAPLLLVGMVDPYIHSSFVSNLVEGGIRLVVFLLYIWGIGF
                     MPDIRRVYAYHGAEHKCINASEAGEPLEASRAVPYTTAHPRCGTSFLLIVMAISILIF
                     ALFGRPPMALRIVSRIVLVPVIAGVAYEFLKFTAAYYSNILVRWMAAPGLTLQRLTTR
                     EPDESMLEVGMAALVEVMRLDAIEESEAEVKPETVPAATNP"
     gene            complement(3100..3870)
                     /gene="hisF"
                     /locus_tag="VHS06_11430"
     CDS             complement(3100..3870)
                     /gene="hisF"
                     /locus_tag="VHS06_11430"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX2988768.1"
                     /translation="MLTKRIIPCFDVNGGRVVKGVNFVNLRDAGDPVELAVRYEQEGA
                     DEIVFLDITASSDNRDNMVDVVRKTAEVLFVPLTVGGGIRSVDDARRLLKAGADKVSV
                     NTAAVKDPSVISRGADLFGAQCMVVSIDAKRRTPGDPASGWEVYTYGGRTSTGIDALE
                     WAQKVVGLGAGEILLTSMDRDGTQNGYDLELLSAVSKAVTVPVIASGGAGSPSHLYDA
                     LARGGADAVLAASIFHYGTYSVRDVKRYLADRGLPIRL"
     gene            complement(3860..>4577)
                     /gene="hisA"
                     /locus_tag="VHS06_11435"
     CDS             complement(3860..>4577)
                     /gene="hisA"
                     /locus_tag="VHS06_11435"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010874150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX2988769.1"
                     /translation="DLRDGRCVRLFQGQYDQETVFSEDPVAVARQWEAEGAPMLHLVD
                     LDGALAGVPKNLEVIKRIVKAISIPVEMGGGLRDVPSIQRLLEAGVSRAILGTAAVET
                     PELVKRACRRFDDRIVVALDAKDGIVMTRGWTEASGETAVEVAIKMVKLGVRRLMYTD
                     ISRDGTLTEPNWEAGKQLLAEVDIPVVASGGVAKVEQLVRLRNLGFEGAIVGRALYTG
                     DIKMADLRQLGLLPERKDAN"
BASE COUNT          856 a         1406 c         1390 g          925 t
ORIGIN      
        1 aaggccaaga agctcgcctt gactgcctgc atgcacaaac tgctggtcat tctcaacgcc
       61 atggccaaac atcacaccaa gtggctacag ccacagctga tgtcggccca aaactaaccg
      121 tcaaccaaga cagttgctac gtctgtgttc gtcccgcatc ctgttccgcc cacagctgtt
      181 cgatccaggg cgggacggtc gcgtccggcc aacaatcacc tcgggtgagg tagggcttca
      241 agtccagcag aggggtgccg tccagagcgt ccagcccgat cactttcagg gtgggacctc
      301 tacgttccag caggcgaacg gcggtgaatc cgatggggtt cggtcggaac atagtcctga
      361 tggcgaagat tccgacctca ggaaggtgct cctgccgctg tgggtgctgc ttcagcacct
      421 tgccgcgctc cgactcgacc ctgtttagcc agaacagcac gattatgtgg gagaaaccct
      481 cgatgccgtc gagcgcttcc tccagggtcg ggtcgacctc tatggtggat tcgatctcct
      541 cccagggacg attcggtttc tccgtgtaac cgttcctgat gacgccgatg gggcgcaaca
      601 gcatgcctga tggcggttga gtactgtttt gcattgctcg ctctctccta cttgccggag
      661 gcatcgatgg cttgcggcga tcgtgattgt tctggcggca gtcgccgttt gggcggcctt
      721 gtcggtcgcc atgccccggt tgcttctgtc gaaaggtgca gggtgaagcc tatcggcatt
      781 ctacatccca ttgtcggcgg acggcagggt ggtgttgcgg ggttcccaaa ccacagcaat
      841 taccttgaca gtaaccggcc accacggcta taatccctta ttgcggcgcg agtagcaccg
      901 ccttttgttg tgcggggttt cgctccccac ggtctggcga ggcagaccac gctcaattga
      961 cattgcgggc cagggatacc ctggcgcttg cggtgaccag ggaaggtgtt gaatgtacgc
     1021 tgtagttgag acgggtggaa aacagtacag ggtcgaagtt ggacagaccg tggacgtgga
     1081 gcatatcccg gctgaggtcg gggacaccgt cgaactggga cgggttttga tggtctccac
     1141 cgatgacggc gtccgactcg gtagtccggt gttggaaggg gctaaggtca cggcgagcgt
     1201 ggtagatcag cgccgcggaa agaagatcat cgtcttccgg tacaagccga agacacgcta
     1261 tcgcaagaag accggccacc gacaggatct gacccgggtt tcgatccagg gcatcgcact
     1321 ggagtaggag agaactagat ggcacacaaa aaaggtgtag gaagctcacg caacggccgc
     1381 gacagtgcga gccagcgact tgggttgaag gttggcgacg ggcaatacgt ccatgcgggt
     1441 tccatccttg tccgccagcg cggcaccgcg atgcgccctg gggacaacgt gggcctcggc
     1501 aaggaccaca ccctcttcgc cctgatcagc ggcgaggtga agttcggcaa ctatagcaaa
     1561 gagcagaaga aagtcagcat ctacgcagcc ccgacggcgt agtggctgat cggggaggga
     1621 cggttctatg aagcagggta ttcacccgaa gtacgccgag acgaccatcg tttgcgcctg
     1681 cggagcgcag cacaagactt catccaccaa gagcaatctg aggattgacg tctgctccca
     1741 gtgccacccg ttcttcaccg gtgagcagcg catcatggac acggctgggc gagtcgataa
     1801 gttcatgaag aagctgcaga agaaggtccg atagtcagag gacctcgatt gggagagtca
     1861 gagcgccgct ctgcctctcc cttttgccta tttggagcat gttatgagtt ctagcggcaa
     1921 aagcttcaca tacggcgggc aggcagtgct ggaaggcgtc atgatgcggg gcagcaggtg
     1981 gtccaccgtg gccgttcgtg ctccgtcctc cgaaatcgtt ctgcactccg agcacctccc
     2041 caaggcgctg tacgacagct ggttcataaa gatccccttc gtccgcggtg tcggaatgct
     2101 ctgggacaca ctggtactcg gcatgagatc gctgatgttc tcggcgaacg tggcgctcca
     2161 ggaagagggt gaagacgggg agcaaccggc cgagatgagc aagccgatga tgtggggcac
     2221 cgtcgcggtt gccatcctct ttgccatcgg tcttttcttt gtcgctcctc tgcttctcgt
     2281 cggcatggtg gatccctaca ttcactcctc cttcgtgagc aatcttgtcg agggcggcat
     2341 ccggcttgtc gtgttcctcc tgtacatctg gggtatcggc ttcatgcccg acatcaggcg
     2401 ggtctacgcc taccacgggg cggagcacaa gtgtatcaat gctagtgagg caggcgagcc
     2461 gttggaggcc tcccgtgcgg tgccttacac cacggcccat ccccgctgcg gcaccagttt
     2521 cttgctgata gtgatggcaa tctcgatcct catctttgcg ctctttggca ggccgcccat
     2581 ggctctgagg atcgtctcca ggattgtgct ggtgccggtt atcgccggag tcgcctacga
     2641 gttcctcaag ttcaccgccg cctactactc caacatcctg gtgcgctgga tggctgctcc
     2701 tggattgacg ttgcagcggc tgacgacgcg cgagccggac gagtccatgc tcgaggtggg
     2761 gatggctgca ttggtcgagg ttatgcggct ggacgccatc gaggagagtg aggccgaggt
     2821 gaaaccggag accgttcccg ccgcgactaa cccatgaagg ttccttctcg atcgatcttc
     2881 caagtctagt cctctgacac agtactcctg tttctagtgc tctgacacgc tgatcgtgac
     2941 accaggagtc gaggtcgtcc ctgacctcga cggcgccaca ggaaaccgcc cggcgcaacg
     3001 ctgcgctcgc tcgcgttgcg gggacgaccc gggctcctag agatcaagtc catcgatgta
     3061 ggggcgacgc atgcgtcgcc cctgttgagt atctggggac tacagacgga tggggagtcc
     3121 tcggtcggcc aggtagcgct tcacgtcccg gaccgagtag gtaccgtagt ggaagatgga
     3181 ggcggcaagc accgcgtctg cccctccccg ggcaagtgcg tcgtagaggt gagatgggct
     3241 ccctgctcca ccagaggcga tgaccggcac cgtgactgcc ttggatacag ccgatagcag
     3301 ctccaggtcg tagccgttct gagtaccgtc tctatccatg ctggttagca ggatctcccc
     3361 ggctcccaac cccactacct tctgagccca ctccagggca tcgatcccgg tcgaggtgcg
     3421 gccgccgtag gtgtagacct cccaaccgga cgcagggtca ccgggagtgc ggcgcttggc
     3481 atcgatggag acaaccatgc actgggcgcc gaagaggtcg gcgccacggc tgatcaccga
     3541 agggtccttg actgccgccg tgttcaccga aaccttgtcc gctccggctt tcagcagccg
     3601 gcgggcgtcg tccaccgacc gaatgccgcc cccaaccgtg aggggaacga agagcacctc
     3661 agccgtcttc cgcaccacgt ctaccatgtt gtcgcggttg tcgctggacg cggtgatgtc
     3721 caagaagacg atctcatcgg ccccttcctg ctcgtaccga acggccaact ccacggggtc
     3781 gcccgcatcg cggaggttca cgaagttgac gcccttgacg acgcgacccc cgtttacgtc
     3841 aaagcagggg atgattctct tagttagcat ctttcctctc ggggagcagt cccagctgcc
     3901 gaagatccgc catcttgata tcgccggtgt agagggcgcg gccaacaatt gccccttcga
     3961 aacccaggtt gcgcaacctg acgagctgtt ccacctttgc cactccgccg gatgccacaa
     4021 cagggatgtc cacctcggcg agtagctgct ttcccgcctc ccagttgggc tcggtcagtg
     4081 tcccgtcccg gctgatgtct gtgtacatca accggcggac gcccagcttc accatcttga
     4141 tcgcaacttc tactgctgtc tcgcccgacg cctcggtcca gccccgggtc atcactatgc
     4201 cgtccttcgc gtcaagcgcc accacgatcc tgtcgtcgaa ccgccggcag gcccgcttga
     4261 ccaactcagg cgtctccacg gccgcagtcc cgaggattgc cctgctcact ccggcttcca
     4321 gcagcctctg gatggagggc acgtctctca ggccgccgcc catctcgact ggaatggaga
     4381 tggccttgac gatacgcttg atcacttcca ggttcttggg gacgccggcc agggcgccat
     4441 cgaggtcgac caggtggagc atcggggctc cctcggcctc ccactggcgg gcaaccgcta
     4501 cggggtcctc ggagaacacc gtttcctgat cgtattggcc ctggaacagc cggacgcagc
     4561 ggccgtcccg caggtca
//
LOCUS       DATALS010000512         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_75251,
            whole genome shotgun sequence.
ACCESSION   DATALS010000512 DATALS010000000
VERSION     DATALS010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75251"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VHS06_11440"
     CDS             complement(<1..440)
                     /locus_tag="VHS06_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX2988770.1"
                     /translation="MITTIAYRPEKEQPTRPVRVLAIQNAPCVPDKEANIAAMVGWMR
                     QGLKDFAADFVVFCELGTTVYFAGAQEPSWQDLAEPIPGPTTELFSRLAAEYHVNVAL
                     SLYERGPRGGEFYNSTVFLDRRGEIIPGLLPDGKSVSCYRKTHIP"
     gene            complement(443..1813)
                     /gene="hydA"
                     /locus_tag="VHS06_11445"
     CDS             complement(443..1813)
                     /gene="hydA"
                     /locus_tag="VHS06_11445"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX2988771.1"
                     /translation="MSVEQVDTIVRGGRVVLSSGVVEAAIAVNGGKIVAVASESVLPP
                     AANYIDASGQLVLPGLVDTHTHIYLDSYRSISESAACGGVTSLLSYIWPDQAQDIPGS
                     IDHWRRFGEAASVVDFGLHICLQDSPASLEQLPAAMEAGVTSLKLMMGFKRRGMMVSD
                     EFMMAAMEAVGRAGGIASVHCESGGVINYLEEKMVAAGLISPVDFPRSRPAQSEVEAI
                     QRAISIAEMAGCPLYIAQITTADSVDVVADARLSGRQVWAETCPHYLFMTEAEYEHQG
                     PLVKIAPPLRTEADCEALWTGIDRGIISVVTSDHTGYSREMKQGGWANIFQVPFGFPG
                     SETMLPLMYTEGVVKRDLPITTLVKLLSENPAKLFGLFPRKGAIQVGADADLVVFDPE
                     AEITITSSALHTQADFTPFEGRKVKGQVTNTMLRGDLVVKDGQIVELPPHGEFLVRQP
                     NTLTAV"
     gene            complement(1916..>2368)
                     /locus_tag="VHS06_11450"
     CDS             complement(1916..>2368)
                     /locus_tag="VHS06_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988772.1"
                     /translation="RVMMLFAFSLSESVLAAGKKPFQLSDVMKESAILRKAGVNSFLY
                     LTFGGYGETPATVEETISHIPYVAPSYTLMDHGFRIQSGTELRDLAVTQGIISPEDDC
                     FKATFYHSPETPAEMLDARIKKYHRQHRLIGRSGISWATRFVWDKVRP"
BASE COUNT          406 a          719 c          783 g          460 t
ORIGIN      
        1 gggatgtggg tcttgcggta gcagctcacg ctcttcccgt ccggcaacag gccgggaatg
       61 atctcaccgc gccggtccag gaagacggtg ctgttgtaga actccccgcc gcgagggccg
      121 cgctcgtaca gcgagagggc cacgttgacg tggtactcgg cggcgagacg ggagaacagc
      181 tcagtcgtgg gaccggggat cggctcggcc agatcctgcc atgacggttc ctgggcgccg
      241 gcaaagtaga ccgtcgtgcc cagctcgcag aagaccacga agtctgccgc aaagtccttc
      301 agcccctgcc tcatccagcc gaccatggcg gcaatgttgg cttctttgtc cggaacgcag
      361 ggggcgttct gaatggccag caccctcacc ggccgtgtcg gctgttcctt ttctgggcga
      421 tacgcgatgg tggtgatcat tactataccg ccgtcagagt attgggttgg cgaaccagga
      481 actccccgtg gggaggcagt tccactatct gtccgtcctt cacgaccagg tcgcctcgca
      541 acatggtgtt ggtgacctgt cccttcacct tgcgtccctc gaagggggtg aagtcggcct
      601 gggtatgcag cgcgctgctg gttatggtga tctccgcctc cgggtcgaag accaccaggt
      661 cggcgtcggc acccacctgg atggcaccct ttcgagggaa gaggccgaac agtttggcgg
      721 gattctcgct gagcagcttg accagggttg tgatgggcag gtcccgcttg accactccct
      781 cggtatacat cagggggagc atggtctcgc tgccggggaa gccgaaggga acctggaaga
      841 tgttggccca gccgccctgc ttcatctccc tgctgtaccc ggtgtggtcg ctggtgacga
      901 ccgagatgat gccgcggtcg atgccggtcc agagcgcttc acagtcggcc tcggtgcgca
      961 ggggcggggc aatcttgacc agcgggccct ggtgctcgta ctcggcctcg gtcatgaaga
     1021 ggtagtgggg acaggtctcg gcccacacct gccggcctga gagcctggcg tcggcgacca
     1081 cgtccacgga gtcggcggtg gtgatctgcg cgatgtagag ggggcacccg gccatctcgg
     1141 cgatggagat ggcccgctgg atggcctcta cctcggactg tgctgggcgg gagcggggga
     1201 agtctacggg ggagatgagc cctgcggcca ccatcttctc ttccaggtag ttgatcaccc
     1261 cgccactctc gcagtggact gaggcgatgc cgcccgcgcg accgacggcc tccattgcgg
     1321 ccatcatgaa ctcgtcgctc accatcatcc ctcggcgctt gaagcccatc atcaacttga
     1381 gactggtcac cccggcttcc atggccgccg gaagttgctc cagggaggcc ggcgagtcct
     1441 gaaggcagat gtggaggccg aaatccacca cgctggccgc ctcgccgaac cgccgccagt
     1501 ggtcgatgga gccggggatg tcctgagcct gatcgggcca gatgtagctg agaaggctgg
     1561 taacgccgcc gcaggcagcg gactcgctga tcgagcgata gctgtccagg tagatgtggg
     1621 tgtgggtgtc cacgaggccg ggcagcacaa gttgtccgga ggcgtcgatg tagttggctg
     1681 ccggcgggag gacgctctcg gaggccacgg ccacgatctt gccgccgttg acggcgatgg
     1741 ccgcctcaac cacgccggag gacaggacca ccctgcctcc tcgcactatc gtgtctacct
     1801 gttcaacgga cattgccgcc acctcctcaa ttggcttggt cttatggaca tttgcgcctc
     1861 tacagccgac tggctggact gtaacacagg cgcaaatggg ggtcaaactg atctactacg
     1921 gcctcacctt gtcccagacg aagcgtgtcg cccaggaaat cccggacctg ccgatcaacc
     1981 tgtgttgcct gtgatacttc ttgatgcgag cgtccaacat ctccgcgggt gtctcgggcg
     2041 agtggtagaa ggtggccttg aagcagtcat cttccggcga aatgatcccc tgggttacgg
     2101 ctaggtcgcg cagttccgtc cccgactgga tgcggaagcc gtggtccatg agggtgtagg
     2161 agggggcgac gtaggggatg tggcttatcg tttcctctac cgtcgcgggc gtctcaccgt
     2221 agcccccgaa ggtgaggtag aggaagctgt tcacgccggc cttgcgcagg atggccgact
     2281 ctttcatgac gtctgagagt tggaacggct tcttgcctgc tgccagcacc gattccgaga
     2341 ggctgaaggc gaagagcatc atcaccct
//
LOCUS       DATALS010000513        11132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_358565, whole genome shotgun sequence.
ACCESSION   DATALS010000513 DATALS010000000
VERSION     DATALS010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11132
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358565"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VHS06_11455"
     CDS             <1..760
                     /locus_tag="VHS06_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2988773.1"
                     /translation="PEKLAMLQVYGHPVVKLKWNSFSEVTGMMEAAIRDLGMYQFVNF
                     LNPFRHEGGKTYAYEISLDLGWRVPDRLIQPIGTGGGVYGAWKGYKELHAIGLVDRLP
                     RMTGVQPEAAAPVCMAFQRGERTAGRHGDPRQTIAQSIAGDSVIQGGRRVLDALYDSG
                     GYGETVSDEEIRAGIALLGSEGIFGEPAGGAVVAAAKKLVRKGIIDPGERIVLVVTGT
                     GLKQPHAVQDYFPEPEVMQASPGDLESLVKRVWG"
     gene            873..1928
                     /locus_tag="VHS06_11460"
     CDS             873..1928
                     /locus_tag="VHS06_11460"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEX2988774.1"
                     /translation="MQTRDRTSPPLTPEQIRELIDRVPRVRLTPLPTPLQECPRLSAA
                     LGGPRILVKRDDLTGLAFGGNKTRNLEFRMAEARDLGADVFIAGLEVQSNSARQTTAA
                     ANVLGMKPVLLLRVDGDWDCQGNLLVDLILGADIHFLESDSAADMDRKLRELAEEYRR
                     RGHRPYVMNHAGGFALGSALAYILSTLEVIEQTAPLGAKPTHLYMSSGSKGQAGLELA
                     RRLLGADFRVVGISARANASRLEDTARIANDAADRLGVDVHIQPDDITNFDEYAAPGY
                     GKPSEAGLEAIRLMARTEGLLLDPIYTGKAMAGLVDHVRRGLLTREDTVVFMHTGGLP
                     ALFAHKDAIVTYMRESR"
     gene            1979..2452
                     /locus_tag="VHS06_11465"
     CDS             1979..2452
                     /locus_tag="VHS06_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2988775.1"
                     /translation="MTPEERLTARGMELPAPPTPVGNYVPAIRVGNLVFLSGHGPNQP
                     NGPQWQGKVGQNLDIEQGQAAAQSAMLNLLASLKAEIGELSRVKRVIKLLGMVNCTPD
                     FGAQPKVINGASDLLVDLFDDRGRHARSAVGMASLPNNIPVEIEMIAEVATVSED"
     gene            complement(2571..3584)
                     /locus_tag="VHS06_11470"
     CDS             complement(2571..3584)
                     /locus_tag="VHS06_11470"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX2988776.1"
                     /translation="MAGSIAAAEQDNVLLEVKDLKMYFPITQGILLQRKVGDVRAVDN
                     VSFFIRKGETLGLVGESGCGKSTTGRSILQLYKPTAGSVKFGDTELTSLKGEELRKMR
                     RRMQMIFQDPYASLNPRMTVGDIIGEPLDIHHLAKGKARQDRVQELLRLVGLNPYFAN
                     RYPHEFSGGQRQRIGVARALAVEPEFIVCDEPVSALDVSIQAQIINLLEELQQEFGLT
                     YLFIAHDLSVVRHISDRIAVMYVGKMVELAGRNSLYQRPLHPYTKALMSAVPVPDPEV
                     EARRQRIILTGDVPSPVNPPSGCRFHPRCRYAREECSQREPEFREVETDHWVACHFWD
                     QIA"
     gene            complement(3574..4566)
                     /locus_tag="VHS06_11475"
     CDS             complement(3574..4566)
                     /locus_tag="VHS06_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988777.1"
                     /translation="MGRLLEIEDLRTSFYTQDGVVKAVNGVSYGLDEGETLGLVGESG
                     CGKSVTALSIMRLIPQPPGKIVSGRIVFEDRDLLKLSEDEMRRVRGKEIAMIFQDPMT
                     SLNPVLTVKRQLTEGLELHLGMDGKAATKRAIELLDMVGIPSARSRVNDYPHQFSGGM
                     RQRVMIAMALACNPKLLIADEPTTALDVTIQAQILDLIKNLRKEFGTAVIMITHDLGV
                     VAGLCDRINVMYAGFIVESASASDLFKDPRHPYTLGLLRSIPRIDEERREKLIPIDGL
                     PPDLIDSPPGCPFAPRCVYAVAKCREENPRLEPVSRGHRIACWVDVTGGVQDGR"
     gene            complement(4597..6033)
                     /gene="gcvPB"
                     /locus_tag="VHS06_11480"
     CDS             complement(4597..6033)
                     /gene="gcvPB"
                     /locus_tag="VHS06_11480"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX2988778.1"
                     /translation="MTEPLLSEISRPGRIGHHLPDSDVPPAEESLPAGLLREALDLPE
                     VSENEVVRHFVHLSHLNYGLDTGFYPLGSCTMKYNPKMSEEIARWPGFSAIHPLQAEE
                     SVQGALRLMYELQRLLGEISGLRAVSLQPAAGAHGELTGMLMIRAYHESRGDRKRTKV
                     LIPDSAHGTNPATATMCGYSTVPVRSDARGNVDLEALSTLVDGETAALMLTMPNTLGL
                     FDENMLAISETVHGCGALMYGDGANMNAMLGVAKPADLGFDVLHINLHKTFATPHGGG
                     GPGAGVVAAREMLEQFLPVPFVVKTPEGRYSLNCGQRESIGQVRSFYGSFGVLVRAYA
                     YIRALGAEGLRKVGENAILNANYLRTRLQQAYEVPYPRSCMHEFVLSGSRQKAQGVRT
                     LDIAKRLIDFGFYPPTVYFPLIVEEAMMIEPTETESLETLDAFADALLAIAAETEENP
                     EVVKAAPTNTVVGRLDETAAARQPRLHW"
     gene            complement(6030..7394)
                     /gene="gcvPA"
                     /locus_tag="VHS06_11485"
     CDS             complement(6030..7394)
                     /gene="gcvPA"
                     /locus_tag="VHS06_11485"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX2988779.1"
                     /translation="MDFVPNTDADRQQMLRAIGVQTIDDLLNVVPETVRHPSLRLPEP
                     MTEMELMAEMRAMAGQNADAQHFACFLGAGAYHHYIPSAISQLLLRSEFYTAYTPYQA
                     EISQGTLQSAFEFQSMICSLFGMEVTNSSMYDGATSLAEAALMAVAATHRRKVVVSGT
                     VNPEYRAVLKGYMQSRGVQVVASAVAREGDRIVEEPLEQILDDQCACCIVQQPNFLGG
                     VRDLAAIADRTHEVGALFIVSADPISLGLLKSPGEWGADIAVGEGQGLGNAMSFGGPY
                     LGLLACRERLVRQMPGRLVGATTDAKGRRGFVLTMQAREQHIRREKATSNICTSEQLL
                     ALSAAIYMSLMGPVGLRKVAQLCYDKAHYAAKGVASVKGYRVLDTGSFFNEFVVECPL
                     PPAEISHELLNRGIIGGYDLAVLGPSLKGCMLMCATETNTRGQIDGLASALSEIAASQ
                     GGVR"
     gene            complement(7489..7869)
                     /gene="gcvH"
                     /locus_tag="VHS06_11490"
     CDS             complement(7489..7869)
                     /gene="gcvH"
                     /locus_tag="VHS06_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX2988780.1"
                     /translation="MVPKELRFSKEHEWVKVEGGEAVIGISDHAQEELGDVVYVELPS
                     VGQQLEQFKTFGVVESVKAASDLYSPVSGEVIAVNSDLTKSPEKVNEDPYGSGWMIRL
                     RLKDSSELNNLMDSAAYESEIAKG"
     gene            complement(7871..9016)
                     /gene="gcvT"
                     /locus_tag="VHS06_11495"
     CDS             complement(7871..9016)
                     /gene="gcvT"
                     /locus_tag="VHS06_11495"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX2988781.1"
                     /translation="MMDDGTLSRTPLYDLHVAAGAKMVEFGGWLMPVQYSGIIEEHRK
                     VRSTVGLFDLSHMGRISVSGPDCAQYLQALATNDLGKLEPGKVQYSLICNRSGGVRDD
                     ILVYCLGKSEYLLVVNASNRQKVLGWMGEIKEGLKEQKGSADVDIEDQTVETVMVGLQ
                     GPLSQQTLQPLTSLLLDPVRYYRSSLGTVMGVEGLISRTGYTGEDGFEIIVPADAGSA
                     LWEELARAVAARNGVLAGLGARDTLRLEAGMPLYGHELDETINPFEVRLDRYVHLEKS
                     EMVGREALQEVASSGPKRCLIGLEVSDRAIARQGTPVMLDGVEVGRVTSGSYSPTLDL
                     SIAMALVRSDVAAARPSLCVSVRGGSHEAHVVDMPFYRRAKSKLGES"
     gene            complement(9013..9510)
                     /locus_tag="VHS06_11500"
     CDS             complement(9013..9510)
                     /locus_tag="VHS06_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX2988782.1"
                     /translation="MFEAEVAAETSSTTVACRKARIGDVPVMHRLVNEHAEAGVMLGR
                     PLSELYENLRDFWVIDDEDGVVIACCSLHINWADLAEVRSLAVDKRYQRRGLGSRLVA
                     ACLEDAQRMGLGRVYALTREATFFESVGFTRIGVNELPRKVWGECIRCPKFPECDEVA
                     VVRDL"
     gene            complement(9492..11084)
                     /gene="recN"
                     /locus_tag="VHS06_11505"
     CDS             complement(9492..11084)
                     /gene="recN"
                     /locus_tag="VHS06_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX2988783.1"
                     /translation="MFVLPTEQAGEAGAAASALEEYLGEHGLEMEEGLLILSREINRT
                     TGRTVARINSRPVPLSLLQRVGQLLVDIHGQSEHLSLLRVKEHVDYLDRYAVLMVLRG
                     EVAETVKELRGVRAEIEQMTRDERESAREMDLLRYQIEEIQAANLQEDEEERLQVERV
                     RLRNTGRLRETAHAAYEQLGGGDDQPGAMDLLAQAERLYVELARLDPSLEEDRLALET
                     ARYAVEEIAGKARDYVEAVEDDPQQLNLVEERIELIHELKRKYGRTLADVINYCRDAE
                     AKLERLSNLAFYLGDLQEKEGGLLDRLGKLASRLSSSRKAAAGQLAEEVQRELGALNM
                     GGTRFQVSFSTIDDPHGVVYETNGSESGNGGARVAFDATGVDRVEFLVAPNPGEEPKP
                     LAKVASGGEMARLMLALKTILCKADAIPTLVFDEIDVGVGARSGTRVGEKLWMLTEDH
                     QVLCITHMPQIACMADRHLSVAKLVEGGRTHTAVRALEGRQRVQELAAMLAGTAEFAS
                     AVASAEELLGRSLEYKECSRQR"
BASE COUNT         2110 a         3788 c         3125 g         2109 t
ORIGIN      
        1 tccagagaag ctggccatgc tccaggttta cggccacccc gtggtgaagc tgaaatggaa
       61 cagcttctcc gaggtcacgg ggatgatgga ggcagccatt cgcgacctgg ggatgtatca
      121 gttcgtcaac ttcctgaacc catttcgcca cgaaggtggc aagacctacg cctacgagat
      181 ctccctggac ctggggtggc gggtaccgga tcggttgatc cagcccatag ggactggagg
      241 cggagtgtac ggagcctgga aaggctacaa ggagttgcat gccatcgggc tggtcgaccg
      301 gcttcccagg atgaccgggg tacagcctga ggccgcggcg ccggtgtgca tggccttcca
      361 gaggggagag agaacggctg gacggcacgg ggatccccgc cagaccattg cccagagtat
      421 cgcgggcgac agcgtcatcc agggaggcag gcgggtgctg gacgcgctgt acgactccgg
      481 cgggtacggg gagacggtga gcgacgagga aatccgggcg ggcatcgctc tcctcggcag
      541 tgagggcatc ttcggcgagc cggccggtgg ggcagtggtg gccgctgcaa agaaactcgt
      601 gcgcaaaggc attatcgacc ccggggaaag gatcgtcctg gtggtcacgg ggacaggtct
      661 aaagcagccc cacgcggtcc aggactactt tccggaaccc gaggtcatgc aggcatcgcc
      721 gggggacttg gagtcactcg tgaagcgagt gtggggctaa tccctcgccg actcgttctc
      781 ggaaggggga aatcctctcc ggtcccgatg cacccacggc gaaaccaaag gcaatggctg
      841 gctctcgcgg ttagaagaca aggagatact ggatgcaaac tcgagacagg acctcgcctc
      901 ccctcactcc cgagcagatc agggagttga tcgacagagt tccccgcgtg cgacttacac
      961 cgctgccgac accccttcag gagtgtcctc ggctatcggc tgccctcggc gggccgcgca
     1021 tccttgtgaa gagggacgac cttaccggtc tcgcctttgg aggcaacaag acgcgcaacc
     1081 tggagttccg tatggccgag gccagggatc tgggcgcgga cgtcttcata gccggcctgg
     1141 aggtccaatc caactccgcc cgccagacca cggccgcggc gaacgtactc ggtatgaaac
     1201 ctgttctgct gctccgggtg gacggtgact gggactgcca agggaacctc ctggtagacc
     1261 tcattctcgg cgccgatatc catttcctcg agtccgacag cgcggccgac atggaccgca
     1321 aactccggga gctggccgaa gagtatcgcc ggcgtggaca ccgaccgtac gttatgaacc
     1381 atgccggcgg cttcgcgctt ggctccgcgc tggcctacat cctgtcgacg cttgaagtta
     1441 tcgaacagac cgcgccgctg ggcgctaagc caacccacct gtacatgagt tcgggcagca
     1501 aggggcaggc gggcctcgaa ctggcaaggc ggctgctcgg cgctgacttc cgggtcgttg
     1561 ggatcagcgc tcgggcgaac gccagccgac tcgaggatac ggcccgcatc gccaacgacg
     1621 ccgctgatcg gctgggcgtg gacgtccaca tacagcccga cgatatcacc aatttcgatg
     1681 agtatgctgc gcccggctat gggaagccat ctgaggcagg gctcgaggcg atccgcctga
     1741 tggccaggac agagggactc ctgttggatc ccatctacac aggcaaggct atggcgggcc
     1801 tcgtggatca cgtgcgccgt ggcctcctca ctcgagagga taccgtggtg ttcatgcaca
     1861 cgggtggcct ccccgcactc tttgcccaca aggatgcaat cgtcacgtac atgcgcgaaa
     1921 gccgctagac actgtcgtgc aaccatttcg agagagccac aaggcaaagg agcccacgat
     1981 gacacctgaa gaacggttaa ctgcccgagg catggaactg cctgctccac ccacacctgt
     2041 ggggaattac gtcccggcca tacgggtcgg caacctcgtc ttcctatccg gacacggacc
     2101 taaccaaccc aatgggcccc agtggcaggg taaggtgggc cagaacctcg atattgagca
     2161 gggtcaggct gctgcccagt ctgcgatgct gaacctgctg gcgtcactca aagccgaaat
     2221 cggcgagctg tccagggtca aacgcgtcat caagctactg ggcatggtga actgcacacc
     2281 ggacttcggc gcccaaccca aggtgatcaa tggagcgtcg gatctcctgg tagacctgtt
     2341 cgacgatcgt ggacgccacg ctcgatccgc ggtgggcatg gcctccctac ccaacaacat
     2401 ccccgtagag atagagatga ttgccgaagt cgctactgtg tcagaggatt aggtcgcggt
     2461 tggcgcagca cacgtacaac agacggcaac aaacaggggc tgctggaaat ccctccagca
     2521 gcccctcgat gctcatatcg cgtctgatga accgacccta cgccactccc ctatgctatc
     2581 tggtcccaga agtggcaggc aacccagtgg tcggtctcca cttccctgaa ctccggctcc
     2641 ctctgcgagc actcctccct cgcataacgg caccggggat ggaaccgaca gccgctcggg
     2701 gggttcaccg gactcggaac gtcccctgtc aggatgatcc gctgacgcct agcctcaacc
     2761 tcggggtccg gcacaggcac cgcgctcatc agcgccttgg tatacgggtg cagcggccgc
     2821 tggtacagtg aattcctgcc cgccagctcc accatcttcc caacgtacat caccgctatc
     2881 cggtcgctta tgtgccgaac cacgctcagg tcgtgggcta tgaacaggta ggtcagcccg
     2941 aactcctgct gcaactcctc cagaaggttg atgatctgcg cctgaatact cacgtccagc
     3001 gcgctcaccg gctcgtcgca cacgatgaac tccggctcca ctgcaagcgc tcgcgccacc
     3061 ccgatcctct gccgctgccc tccactgaac tcgtgcgggt aacggttggc gaagtatggg
     3121 ttcagcccca ccagccgcag caactcctgc acgcgatcct gcctggcctt gccctttgcc
     3181 aggtggtgta tatccagcgg ctccccgatg atgtccccca ccgtcatcct cgggttcagg
     3241 ctcgcgtacg ggtcctggaa gatcatctgc atccgacgcc gcatcttgcg cagctcctcg
     3301 cccttcagcg acgtcagctc ggtatccccg aacttcacgc tccccgccgt cggcttgtac
     3361 agctgcagga tgctccggcc tgtcgtgctc ttgccgcagc cgctctcgcc caccagcccc
     3421 aacgtctccc ccttcctgat gaagaagctg acgttgtcca cggcccgaac gtcaccgacc
     3481 ttcctctgga ggaggatccc ctgcgtgatg gggaagtaca tcttgagatc cttgacctca
     3541 aggaggacgt tgtcttgctc tgccgcggcg atgctaccgg ccatcctgga cacctcccgt
     3601 cacgtccacc caacaagcga tgcgatggcc ccgcgacacc ggctccagcc tcgggttctc
     3661 ctcccggcac ttcgccactg cgtacacgca tctgggggcg aaggggcatc ccggggggct
     3721 gtctatcagg tcgggcggaa gtccatcgat ggggatcagc ttctcccgcc gctcctcatc
     3781 tatcctgggg atactccgca gcaatccgag ggtataggga tggcgcggat ccttgaacag
     3841 atccgaggcc gaggcgctct ccactatgaa ccccgcgtac atcacgttga tccggtcgca
     3901 caggccggcc acaaccccaa ggtcgtgggt gatcatgatc accgccgtgc cgaactcctt
     3961 gcgcaggttc ttgatgaggt ccaggatctg cgcctggatc gtcacgtcca gcgccgtcgt
     4021 cggctcgtcc gcgatcagca gcttcgggtt gcaggccagc gccatcgcga tcatcacccg
     4081 ctgccgcatg cccccgctga actgatgggg gtagtcgttg accctgctcc tggccgaggg
     4141 gatgcccacc atgtccagca actcgatcgc cctcttggtg gccgctttgc cgtccattcc
     4201 aaggtggagc tccagtccct cggtcagctg ccgcttcaca gtcagcacag ggttgaggct
     4261 ggtcatcggg tcctggaaga tcatcgcgat ctccttgccc cgcacccgcc gcatctcgtc
     4321 ctcggagagc ttcagcaggt ccctatcctc gaagacgatc ctgccgctca ctatcttccc
     4381 aggaggctgt gggatcagcc tcatgatgga gagcgcggtc acactcttgc cgcaaccgct
     4441 ctcacccacg agtcccagcg tctccccctc gtccaaccca taggagacgc cgttaacggc
     4501 cttcaccaca ccgtcctggg tgtagaagct cgtcctcagg tcctcgatct ccagcagtcg
     4561 gcccatgcgt gtgacacctc tcctaggagc tggatcttac cagtgaagcc gaggctgtct
     4621 tgcggcggca gtctcgtcca gtcttcccac gacggtatta gttggagcgg ccttcaccac
     4681 ctctgggttc tcctccgtct cggcggcaat ggccagcaga gcgtcggcaa acgcatccag
     4741 tgtctccagg ctctcggtct cggtaggctc aatcatcatg gcctcctcga cgatcaacgg
     4801 gaagtacact gtgggcgggt agaaaccgaa atcgatgagc cgcttggcaa tgtccagagt
     4861 ccggactccc tgggccttct gtctggaacc cgagaggacg aactcgtgca tgcaagaccg
     4921 ggggtagggc acctcgtagg cctgctgcaa ccgcgtgcga aggtaattcg cattcagaat
     4981 agcgttctcc ccgactttgc gcaagccctc cgcgcctagc gcccttatgt acgcgtaagc
     5041 acggaccaac acgccaaagc tcccgtagaa gcttctgacc tggccgatcg actctcgctg
     5101 cccgcagttc agactgtagc ggccctcggg tgtcttgacc acgaagggca cagggagaaa
     5161 ctgctccagc atctcacgag cggccacaac acccgctccc ggcccgccac caccatgggg
     5221 tgtggcgaag gtcttgtgca ggtttatgtg caacacgtcg aagcccagat cggccggctt
     5281 ggccaccccg agcatcgcgt tcatgttggc gccgtcccca tacatcagcg caccgcaccc
     5341 gtggaccgtc tccgatatcg ccagcatgtt ctcgtcgaac agccccaggg tgttgggcat
     5401 cgtcagcatc agggccgccg tctctccgtc cacaagcgtg ctcagcgcct ccaggtccac
     5461 gttgccgcga gcgtccgaac gcaccggcac ggtggagtag ccacacatcg tcgcggtggc
     5521 tgggttggtt ccgtgagcgg agtcggggat gagcaccttc gtgcgtttcc gatcgccccg
     5581 cgactcatgg tacgccctga tcatcaacat gccggtcaac tcaccgtgag cacctgccgc
     5641 cggctgcagc gagacggccc gcaacccgga gatctcccca agcaaccgct gaagctcgta
     5701 catgagcctc agcgcgccct gcacactctc ctcggcctga aggggatgga tggcagagaa
     5761 acccggccat cgcgctatct cctcagacat cttcgggttg tacttcatgg tgcaggaacc
     5821 cagcggatag aagccggtgt ccagtccata gttgagatgc gacaggtgga cgaagtggcg
     5881 aaccacctcg ttctccgaca cctctgggag gtcgagcgct tcgcgaagca agccggcagg
     5941 aagagactcc tcagcgggcg ggacgtcgct atccggaagg tgatgaccaa tgcggcccgg
     6001 tcgcgagatc tctgaaagca gtggttcagt catctgacac ctccctggga tgcagcgatc
     6061 tcgctcagag ccgaagccag gccgtcgatc tgtccccgcg tattggtctc ggtggcacac
     6121 atcagcatgc accccttcag cgacgggccg aggactgcca ggtcgtagcc gcctatgatc
     6181 ccccggttca acagctcgtg actgatctcc gccggtggga gcggacactc gacgacgaac
     6241 tcattgaaga atgagccagt gtccagcacc ctgtagccct ttaccgacgc gacacccttc
     6301 gccgcatagt gggccttgtc gtagcacaac tgagccacct tacgcaggcc aacggggccc
     6361 atcagcgaca tgtagatcgc ggcactcagc gccagtagct gctcgctggt gcagatgttg
     6421 gaggtcgctt tttcccggcg gatgtgctgc tcccgagcct gcatcgtcag aacgaagcct
     6481 ctgcgaccct tggcgtccgt ggttgcccca accaacctgc cgggcatctg cctgacaagc
     6541 cgctcgcgac acgccaacag tccgagatag ggccccccga agctcatcgc gttgcccagg
     6601 ccctgcccct cgcccaccgc aatgtcggct ccccactccc cagggctctt aagcaacccc
     6661 agggagatcg gatcggccga aacgataaag agggctccga cctcgtgagt gcgatccgca
     6721 atggcagcca ggtccctcac accgccgagg aagttgggct gttggacgat gcaacacgcg
     6781 cactggtcgt ccaggatctg ctccagaggc tcttctacga ttcggtcgcc ctcgcgggcc
     6841 accgctgagg ccaccacctg cactccacga ctctgcatgt agcccttcaa caccgccctg
     6901 tactcagggt ttacggtgcc ggataccact accttcctcc ggtgtgtggc ggcgaccgcc
     6961 atcaacgcgg cctcggcgag ggaagtggca ccgtcgtaca tggaagagtt ggtgacctcc
     7021 atgccaaaca gagagcagat catgctctgg aattcgaagg ccgactgcag cgtgccctgg
     7081 ctgatctccg cctgatacgg ggtgtaggcc gtatagaact cactgcgcag caatagctgt
     7141 gatatggcag atggtatgta atggtggtag gccccggccc cgaggaagca ggcgaagtgc
     7201 tgcgcatcgg cattttgccc agccatcgcc cgcatctcag ccatgagctc catctcggtc
     7261 atcggctccg gcagtctgag cgatggatgc cggacggtct ccggcaccac attcaacagg
     7321 tcatcaattg tctgaacccc gatggcccgc agcatctgct gccggtcggc atcggtgttc
     7381 ggtacgaagt ccatgcgcaa tcacctccgc caaacaggga cttagcggct ttcgatggac
     7441 gctgtcaacc tctccgccgg ctaaaggata ccagcaagag aaagtcgctt agcccttggc
     7501 tatctccgac tcgtaagcgg ccgaatccat caagttgttc aactccgagg agtccttcag
     7561 gcgcaagcgg atcatccatc cgctaccgta tgggtcctcg ttcacctttt ccggcgactt
     7621 cgtgagatcg ctgttcaccg cgatgacctc accgctgacc ggtgaataga gatccgacgc
     7681 cgccttcacg gactcaacca caccaaaggt cttgaactgc tccaattgct ggccaaccga
     7741 cggtaactct acgtagacca cgtcccccag ttcctcctga gcgtggtcgc tgatgccaat
     7801 gacggcctcg ccgccctcga ccttgaccca ctcgtgctcc ttggagaagc gcagttcctt
     7861 cggtaccatc tcaactctcc cctaacttac tcttcgccct tcgataaaac ggcatatcta
     7921 ccacgtgggc ctcatgagat cctcccctga ccgaaacaca cagagaagga cgagcggccg
     7981 ccacgtcgga tctcaccaac gccatggcaa tactgaggtc caatgtcggc gagtaactcc
     8041 cgctggtaac cctgcccacc tcaacgccgt cgagcatcac gggagtgccc tggcgagcaa
     8101 tggccctatc cgacacttcc aacccgatca gacagcgctt cggtccgctg gatgctacct
     8161 cctggagcgc ttcccgaccg accatctccg atttttccag gtggacgtat ctgtccagcc
     8221 tgacctcgaa gggatttatc gtctcgtcca actcatgccc gtagagcggc attccggctt
     8281 ccaaccgaag ggtatctctg gcgccaagac cggcaagcac cccgttccgc gcggcaacag
     8341 ccctggccaa ctcctcccac aatgccgagc cagcatcggc gggcacgatg atctcgaacc
     8401 cgtcctcgcc ggtgtagccc gttcgggaga tcagcccctc cacacccatc actgtgccaa
     8461 gggaggaacg atagtagcgc acgggatcca acaaaagtga cgtaagaggc tgaagcgtct
     8521 gctgcgaaag gggaccctgg aggccaacca tcaccgtttc cacggtctgg tcttcgatgt
     8581 cgacgtcggc ggaccccttc tgctctttca gcccctcctt gatctcgccc atccatccga
     8641 gcaccttctg tcgattggag gcgttgacca ccagaaggta ctccgacttc cccaggcagt
     8701 agaccaggat gtcgtcgcgg acaccgccgg atcgattgca gatgagggag tactgaacct
     8761 tgccgggttc cagtttcccc aggtcgttgg ttgccagcgc ctggaggtat tgagcgcaat
     8821 ccgggccgct caccgagatt cgccccatgt gcgagaggtc aaacagcccg acggtcgacc
     8881 gtaccttgcg atgctcctcg attatgccac tgtactgcac cggcataagc cagccgccaa
     8941 actccaccat cttggcgccg gcagccacgt ggagatcgta tagtggggtc cgactcaggg
     9001 tgccatcgtc catcatagat ccctcaccac cgccacttcg tcacactcgg ggaacttggg
     9061 gcaacgtatg cactcgcccc acaccttccg aggaagttcg ttgacgccga tccgagtgaa
     9121 gccaacgctc tcgaagaatg tggcctccct cgtcagtgcg tagacgcggc ccaaacccat
     9181 tcgctgtgca tcttcgagac aggccgcaac caatctggag ccaagacctc ggcgctgata
     9241 ccgcttgtcc acggccagcg agcgcacctc ggccaggtcc gcccagttga tgtggaggga
     9301 acagcaggcg atcacgaccc catcctcatc gtctatcacc cagaagtccc tgaggttttc
     9361 gtacaactcg ctcagaggtc ggcccagcat gacccccgcc tcagcgtgct cgttgaccag
     9421 cctgtgcata accggaacgt cgccaatccg tgccttgcgg cacgccacgg tggttgaact
     9481 ggtttcagct gctacctctg cctcgaacat tctttgtact ccaacgatct tcccagcaat
     9541 tcctcggcgc tcgctaccgc tgaggcgaac tccgccgtcc ctgccagcat cgccgccaat
     9601 tcctggaccc tctggcgccc ctccagcgcc cttaccgctg tatgggtgcg gccaccctcc
     9661 accaacttcg ccaccgacag atgtcggtcc gccatgcagg cgatctgcgg catgtgagtg
     9721 atgcacagca cctggtgatc ctctgtgagc atccacagtt tctcgccaac cctggtaccg
     9781 cttcgcgcac ctacccccac gtcgatctcg tcgaacacca gagtggggat cgcgtctgcc
     9841 ttgcatagga tcgtcttcag ggccagcatc agcctggcca tctcaccacc actggccacc
     9901 ttcgcaagcg gcttcggttc ctcgccggga ttgggtgcca cgaggaactc cacacggtct
     9961 actccggtcg cgtcgaacgc tactctcgcg ccgccattgc cggactccga cccgttggtc
    10021 tcgtacacca ccccgtgtgg atcgtcgata gtggagaagc tgacctggaa gcgggtgcca
    10081 cccatattga gtgcccccag ttcccgctgc acctcttccg ccaactggcc tgccgccgcc
    10141 tttctggagg acgacaaccg tgaggccaac tttccaagcc gatccagcag cccgccctcc
    10201 ttctcctgga ggtcgccaag gtagaaggcg aggttggata gacgctccag tttggcctcg
    10261 gcatcccgac agtagttgat cacatctgcc agggtgcgac cgtacttccg cttcaactcg
    10321 tggatcagct cgatccgctc ttccaccagg ttgagctgct gagggtcgtc ctccaccgcc
    10381 tccacgtagt ctcgtgcttt ccccgctatc tcctcgacgg cataacgagc cgtctccaac
    10441 gccagtctgt cctcttcaag gctggggtca agcctggcaa gctccacgta gagtctctcc
    10501 gcctgcgcca acaaatccat cgctcccggt tggtcgtcgc caccgcccag ctgctcgtag
    10561 gccgcgtggg cagtctccct gagccgcccg gtgttgcgca atctgacccg ttcaacctgc
    10621 agccgctctt cctcatcttc ctgcaagttc gcggcctgaa tctcctcgat ctgatagcga
    10681 aggaggtcca tttccctggc cgattccctc tcgtcccgcg tcatctgctc gatctcagcg
    10741 cggacccctc gcagctcctt gaccgtctcg gccacctcgc ctcgcagcac catcagcacg
    10801 gcgtaccggt ccaaatagtc gacatgctcc ttgacccgca gcagcgatag gtgctcgctc
    10861 tgtccatgga tatccaccag caactgcccg acccgctgta gcagcgacag cggcacgggc
    10921 cggctgttga tacgcgcaac ggtccttccc gtcgtccggt tgatctcccg gctcaggatg
    10981 agaaggccct cctccatctc caacccatgc tctcccagat actcctccag agccgaggcc
    11041 gccgctccgg cttcacctgc ctgctcagtg ggcagaacga acaccgcccc gctcgtgagc
    11101 tacgtggccg atgggctgat cctggcctcg cc
//
LOCUS       DATALS010000514         4821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_359236, whole genome shotgun sequence.
ACCESSION   DATALS010000514 DATALS010000000
VERSION     DATALS010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359236"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VHS06_11510"
     CDS             <1..854
                     /locus_tag="VHS06_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988784.1"
                     /translation="TVLGVLMVLHPSLSRTVWALTSGAPIRTADGRILGAVLTVTDIT
                     AIRRLEEQRSAYILGISHGLRTPLTVILGQAQLLQRAAEKCALTGRERTGVEAIVANA
                     RRMGVMLRDLIDLTEIDAGKPLKLNRVLLNPRSFVLELTQQSIGFLETARILVESPED
                     LPSVSTDPDRLQRILINLLSNALKYSHPGSTVRVTLAQRDGEVVVAVSDRGQGIPPEE
                     MELLFQPYRRVQLARGRRESLGLGLYITKGLVEALGGRIWVESKVGEGSTFSFTLPMA
                     EPSQSSS"
     gene            1010..2221
                     /locus_tag="VHS06_11515"
     CDS             1010..2221
                     /locus_tag="VHS06_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2988785.1"
                     /translation="MTADNHLGRYYDRMLPNRLEERRAWLRRGFAASVECALERHAHL
                     FLHAGDLFDGAEPRNVERQFVARQLARLKDAGIPCLAIGGNHDTPRSRNGRSAATAQG
                     TYAYLDGLRLLGECPEDGDQHSALSTQHSVAGRVGGVDSETLTIDRVRLAVGGIAPDP
                     TAPAGSDPLAGLQWHPEADVALLMIHGSLEGHIFPGSLEPTVRRESVERLEGVDLLLA
                     GHIHRYSAFRWGHCTVVIPGATERMTFASDEERPGFAYIEMEPGRIVRLEQVAVESQP
                     RRSLTVPTSLLSEGDPAEQLMARLEPLCDPETMVRLSLEGPISRQRYHDLKLRELVEF
                     GVARCFSLELDTSGLYMEDDLAPGSARGGRLSQREELLRYAAECREVADNDRERGLID
                     EALLEILEEYG"
     gene            2218..3939
                     /locus_tag="VHS06_11520"
     CDS             2218..3939
                     /locus_tag="VHS06_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2988786.1"
                     /translation="MILLQRLQVENFKLLRRLDLSFPRRGSILIEGLNESGKSTLFES
                     VYFALYGVPLVAEGRGRGNLDSVIRYGADAASVELAVEVDQTRLEIRRVVSRSRSTQA
                     ALVISRREAPEERVSGVQAVNARVVEELGGMDGDALLNSCFVEQKKLGKLEEMGAAQR
                     RESLRRLLNLERLTVVADRFKVTAQDERALAAAKDRLDMAEVAAMLPKLADERRMLEA
                     HLGGIRLAEGSVGERYATPSPNTVIRDVGATHASPLQELGSELPADPDDEQVESPVEM
                     ADRLESLRASLATTKRRVLLLSGLAFLALLLSALLAAVASVPWLWAPPTILLLVAVAI
                     LTNRRAAGADREIASLEFGLARHRDTLMARLGDLEGRRRRAMERLGGNEEELDPEECR
                     RRVERLSRHLEVKWRAAALVEGTMERIVRLVLPKTERNLGLILPLLTAGRYHEARIGD
                     DYQLRVWDDSAGRYVSKNIFSGGARDQISLALRLSFALATLPQELGATPGFLFLDEPL
                     SSFDGPRTEALVRLLTSGQIAESFDQIFVISHNRSFDPGAFQHRLVLRDGRVVESTIS
                     NVVPGQE"
     gene            complement(4065..4721)
                     /locus_tag="VHS06_11525"
     CDS             complement(4065..4721)
                     /locus_tag="VHS06_11525"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX2988787.1"
                     /translation="MSELLAWSAAMVSLVWIVQAVANMQRHPQGSATKRTSSPLELMS
                     GFVYPILVGSSTATLLLGAGRFQWGWPDALLQLIGLLASIAGLVVYIRAKVELGESYS
                     ADARVWMEQQLITSGLYGLVRHPIYFGSILVWLGIGLGIASWTTLGLTSAILVPAIIY
                     RSRLEERLLRERFGRQYTAYRSRVPMLFPWPRPEKTTQEDDLAELVEMADQPIVPEQE
                     "
BASE COUNT          863 a         1469 c         1571 g          918 t
ORIGIN      
        1 agaccgttct tggagtcttg atggtgctcc atccgtctct cagtaggact gtatgggccc
       61 tgaccagcgg agcgccgatt cgcacagccg acggccgaat actgggggct gtgttgaccg
      121 tgaccgacat aactgcgatt cgcaggctgg aggagcagcg gtcggcttac atcctcggca
      181 tctcgcacgg tttgcgaacg ccgctgacgg tcatactggg gcaggcgcag cttctacagc
      241 gtgcagcaga gaaatgtgct ctgaccgggc gagagcggac gggcgtggaa gccatcgtcg
      301 cgaatgctcg gcggatgggt gtgatgcttc gcgacctgat cgacctcacc gagatcgatg
      361 ccggaaagcc gctgaagctg aatcgggttc tcctgaaccc gcgctccttt gtcctggagt
      421 tgacacagca atcgatcggc tttctggaga ccgcccgcat actggttgag agcccggaag
      481 atttgccatc ggtatcgacc gatcccgacc gactgcagcg tatcctgata aaccttctca
      541 gcaacgccct gaagtactcg catccaggct ctactgtcag ggtgaccctg gcgcagcgtg
      601 atggagaggt ggtcgttgcg gtgtcggaca gggggcaggg cattcctcct gaggagatgg
      661 agctgctgtt ccaaccctat cgcagagtgc agcttgcccg cggccgcagg gaaagcctgg
      721 gactcggtct ctacataacg aagggactgg tggaggccct cggcggccgc atctgggtcg
      781 agagcaaggt gggagagggg agtaccttca gcttcacctt gcccatggcc gaaccgagtc
      841 aatcctcgag ttgaccgggc gcgacgttgg cattcggcgc gaattgccgt ctaacgcggc
      901 gacgggactc tcctccagcg agcgtcccca aaggtggtag aatcttcttg ggctcatttg
      961 gggcctgaca tcctcctcct ggggtgcaac catcaccatt cgtctggtcg tgacggccga
     1021 taatcacctt ggccgttact acgacaggat gctacccaat cgactcgagg aacggcgtgc
     1081 ctggctccgc cgtggctttg ccgcttcggt ggagtgcgcc ctggagcggc acgcccacct
     1141 cttcctccat gcaggagatc tcttcgatgg cgccgagccg cgcaacgtgg agcggcagtt
     1201 cgtcgcccgc cagcttgctc gtctcaaaga cgccggcatt ccctgtctgg caatcggcgg
     1261 taaccacgac actccgcgat cgcgcaacgg ccgctccgcg gccacagcgc agggaacgta
     1321 cgcctacctg gacggtctga gacttctcgg ggaatgccca gaggatggcg atcagcactc
     1381 agcactcagc actcagcact cggttgcagg acgtgtgggt ggggtggaca gcgagaccct
     1441 gacaatcgat agagtgcggc tggccgttgg gggcattgcg cccgacccca ccgcgccggc
     1501 cggctccgat ccgctggccg gtctccagtg gcacccggag gcggacgtcg cgctcctgat
     1561 gatccatggg agcctggagg ggcacatctt tcctggcagt ctggagccga ccgtgcgcag
     1621 ggagtcggtg gagcggctgg agggagtcga cctcctgctg gcagggcaca ttcaccgcta
     1681 cagcgctttc cgctgggggc actgcaccgt cgtgataccc ggtgcgactg agcggatgac
     1741 ctttgcctcc gacgaggaac ggcccggctt tgcctacata gagatggagc ccggacgcat
     1801 tgtgcggctg gagcaggtgg cggtggagag ccagccacgc cgatcgctga ctgtccccac
     1861 cagccttctc tccgagggcg atccggcgga gcagttgatg gctcgcctgg agccgttatg
     1921 cgatcccgag accatggtac ggctctccct ggagggtcct atctcgcggc agcgctacca
     1981 cgacctcaag ctccgggagt tggtggagtt cggcgtcgcc cgctgcttct cactggaact
     2041 ggacaccagc ggtctctaca tggaggatga tctcgcgccc ggctctgctc gcgggggccg
     2101 tctctctcag cgggaggagc tgcttcgcta cgctgccgaa tgccgagagg tggcggacaa
     2161 cgaccgggag cgtggcctga tcgacgaggc attgctggag atcctggagg agtacggatg
     2221 atactgctcc agcgcctgca ggtggagaac ttcaaactgt tgcggcggtt ggacctctcc
     2281 ttcccccgcc gcggctccat cctcatcgag ggactgaacg agtcgggcaa gtccaccctc
     2341 ttcgagagcg tttacttcgc gctgtatggg gtccccctcg ttgcagaggg gcgcggccgg
     2401 ggaaatctgg acagcgtaat ccgctacggc gccgacgctg cttctgtgga gttggcggtg
     2461 gaggtggacc agacccggct ggagatacgc agagtcgttt cgcgctcgcg ctctacccag
     2521 gccgcgctgg tcatcagccg ccgcgaggcg ccggaggagc gggtctctgg ggtgcaggcc
     2581 gtcaacgcca gggtggtgga ggagctgggt gggatggacg gcgatgccct gctcaactcc
     2641 tgcttcgtcg agcagaagaa gctgggaaag ctggaggaga tgggcgccgc ccagcgcagg
     2701 gagtcgttgc gccggctgct caacctggaa aggctgaccg tcgtggcgga tcgcttcaag
     2761 gtcactgctc aggatgagcg ggcgctggcc gcggccaaag accggctgga tatggcagaa
     2821 gtcgccgcga tgctgccaaa gctggccgat gagcgcagga tgctggaggc gcacctgggc
     2881 gggatccgcc ttgccgaagg ctccgtaggg gagaggtatg ccacgcccag tcccaacact
     2941 gtcatccgcg acgtaggggc gacgcatgcg tcgcccctac aggaattggg gagcgagttg
     3001 cctgcggatc cagacgacga gcaggttgag agccccgtgg aaatggccga caggctggag
     3061 tcattgcgcg cctctctggc aaccacgaag cgcagggtgt tactgctgag cgggcttgca
     3121 ttcctggcgc tgctcctctc cgccctgctg gccgcggtgg catctgtgcc atggctgtgg
     3181 gcgccgccca ctatcctcct gttggtcgcc gttgccatcc tgacgaacag gcgcgctgcc
     3241 ggtgcggatc gggagatcgc ctcccttgag ttcggtctcg ctcgacatcg agataccctg
     3301 atggcgaggt tgggcgacct ggaggggcgg cgcagaagag cgatggagcg gctgggaggc
     3361 aacgaagagg aactggaccc ggaggagtgc aggcgtcgcg tcgagcggtt gagccggcat
     3421 ctggaggtaa agtggcgcgc ggctgccctc gtcgagggaa cgatggagcg gatcgtccgc
     3481 ctggtgttgc cgaagaccga gcggaatctg gggctgatcc tgccgctcct cacggcgggt
     3541 cgctaccacg aggctcgcat cggcgatgat taccagttac gggtgtggga cgactcagcc
     3601 ggccgctacg tatccaagaa tatcttctcc gggggggcca gggaccagat atccctcgcg
     3661 ttgcggcttt cctttgcgct ggctaccctt ccgcaggagt tgggcgcgac tcccggtttc
     3721 ctgttcttgg acgagccgct gagctctttc gatggcccgc gcaccgaggc gctggttcgg
     3781 ctgctcacct ccgggcagat cgcggagagc ttcgatcaga tcttcgtgat ttcccacaac
     3841 cgatccttcg atcccggcgc cttccaacat aggctggtgt tgcgcgacgg gcgggtcgtg
     3901 gagagcacca tctcaaacgt ggttcctggc caggagtagg accgatacga acaggatgca
     3961 caggatatac aggatttgca aaaggctaac ctgattaatc tattctgtac atcctgtata
     4021 tcctgtttga tcccgtcctt ccgcaatgcc tttcacctcg cctgctactc ttgctcgggc
     4081 acgatcggct gatccgccat ctccaccagt tctgccagat catcctcctg cgtggtcttc
     4141 tccggccgtg gccaggggaa cagcatcgga acacgggacc gatatgctgt gtactgccta
     4201 ccgaatcgtt ccctgagcag ccgctcttcc aaccgagatc gatagatgat ggcgggaacc
     4261 agtatcgccg aagtcaatcc caaggtagtc cagcttgcta tcccgagccc gattccgagc
     4321 cacaccagga tgctgccgaa gtagatgggg tgccgtacca gcccgtacag cccgctcgta
     4381 atcagttgct gttccatcca cacgcgtgca tcggcggagt agctttcgcc caactccacc
     4441 tttgccctga tgtacacgac gaggcccgcg attgatgcca gtagcccgat tagttgaagc
     4501 agagcgtcgg gccagcccca ctggaacctt cctgcaccca acagcagcgt ggctgtgctg
     4561 gatcccacca agattgggta gacgaacccg gacatgagct caagtgggct ggatgtcctt
     4621 ttcgtcgcgc tgccttgtgg atgtcgctgc atattggcga ctgcctggac aatccagacc
     4681 agggacacca tcgccgccga ccaggccaac agctctgaca tcgagtcccc ctctctcgtt
     4741 tccgatttcc acgccaagga gtggaaggag aaaaagaggc aaacggcagg ccaccgttaa
     4801 ttcgcgctgc aggaccaaga g
//
LOCUS       DATALS010000515         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_75694,
            whole genome shotgun sequence.
ACCESSION   DATALS010000515 DATALS010000000
VERSION     DATALS010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75694"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VHS06_11530"
     CDS             complement(<1..493)
                     /locus_tag="VHS06_11530"
                     /inference="COORDINATES: protein motif:HMM:NF019931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HEX2988788.1"
                     /translation="MALAEDVKGMALEVGFDLVGIASAEPLRDAEVATLGWLREGMAA
                     GMGWITEERVRLSCDPVRLLPGARSIIALARAYPSQLDLDPAHGLTGSVIQREAKDLS
                     SHEEILRSAQDDTRQRPRGRIARYALGDDYHDVLLSRVRQLLDAVSSSLGTRPAGRFF
                     VDSS"
     gene            complement(774..1565)
                     /locus_tag="VHS06_11535"
     CDS             complement(774..1565)
                     /locus_tag="VHS06_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2988789.1"
                     /translation="MSAPGGDKKPMLKKFDLSGKVALVTGASRGIGAGMALALAEAGA
                     DMALVARTEDALESTATRVRALGRKALVVPTDVSKVDSLSGMVERIVAEYGHLDILVN
                     AAGVQCRKPILEVTEADWDFVNSVNLKGLYFVSQAVARQMLKQGKGKIVNICSLTTSI
                     GIANVSIYSATKGAVLSLTRTQSREWSRQGITVNAIGPGYFNTEMTKRLYDDPAMHQW
                     IVDRTPMGRWGEVDDLMGAVVFLSSDASDFISGVLLNVDGGWLSA"
BASE COUNT          341 a          564 c          483 g          305 t
ORIGIN      
        1 gcgaactgtc gacgaagaac cgtccggcag ggcgcgtgcc gagggaggag gagaccgcat
       61 ccagaagctg ccgcaccctg gaaaggagga cgtcgtggta atcgtcaccc agagcatagc
      121 gggcgattcg ccccctcggc cgctgacggg tgtcatcctg agcggagcga aggatctcct
      181 cgtggctgga gagatccttc gcttctctct ggatgacaga tccagtgagg ccatgcgccg
      241 ggtcaagatc caactgactc ggataggctc gcgcgagggc gatgatggag cgagcgccgg
      301 gcagtagccg gaccggatcg caggagagac gcaccctctc ctcggtgatc caacccatcc
      361 ctgccgccat cccctccctc agccagccaa gggtggccac ctccgcgtct cgaaggggct
      421 cggccgaggc gatccccacc agatcgaaac ccacctccag agccatcccc ttcacatcct
      481 cggccagcgc caagatcgcc tcctagtcct aagtagcgaa atgcgacagt acgaaataaa
      541 caggatagac aggatataca ggataaacgg gaattgcccg caaatccatc ctgcccatcc
      601 tgtctatcgc gaccttggca tggcgagagc actgccacta cgaaccgtct gtgcgtcttg
      661 tagggcgggg tcttgtgccc cgcccggagc gtggtcagag tcaccgtgcc ggtcgtggcc
      721 acgttgtggg aggcggggca caagaccccg ccctacgaaa tgatcggcat gtcctacgcc
      781 gagagccagc cgccgtccac gttgagcagg acgccggaaa tgaagtccga cgcatcggac
      841 gagaggaaca ccaccgcccc catcaggtcg tcgacctcac cccaccgacc catcggcgtt
      901 ctgtcgacga tccactggtg catcgcaggg tcgtcgtaca gccgcttcgt catctcggtg
      961 ttgaagtagc cgggcccgat cgcgttgacc gtaatgccct gccgcgacca ctctcgagac
     1021 tgtgtgcggg tgagagacag aacggcgccc ttggtagccg agtagatgct cacgttggcg
     1081 atgccgatag aggtcgtgag ggaacaaatg ttgacgatct tccccttgcc ctgcttcagc
     1141 atctggcgag cgaccgcttg agacacgaag tagagtccct tcaggttgac ggagttcacg
     1201 aagtcccaat ccgcttccgt gacctccagg atcggtttcc gacattgaac accggccgcg
     1261 ttcaccagta tgtccaggtg tccgtactcc gcgacgatcc gttccaccat gccagaaagg
     1321 ctatccacct tggaaacgtc ggtgggcacg accagcgcct tccgacccaa cgcccgaaca
     1381 cgagtggcag tcgattccag tgcatcctcg gtgcgtgcca ccaacgccat gtctgcgccc
     1441 gcctccgcca gggccagcgc cattccagcc ccgatccccc gactggcgcc cgttaccagc
     1501 gccactttgc cggaaaggtc gaacttcttg agcatcggct tcttgtctcc acctggagca
     1561 ctcatcacca ggtctcctta ctgagcttca aattcgcttg gaacggaggc cagtgcctcc
     1621 accacgtact ttacgtagtg gtgacaccgc tcctgcctca gattggtcgc cttgaagcgg
     1681 tcgtactcac cct
//
LOCUS       DATALS010000516        12004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_311358, whole genome shotgun sequence.
ACCESSION   DATALS010000516 DATALS010000000
VERSION     DATALS010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12004
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311358"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2666)
                     /locus_tag="VHS06_11540"
     CDS             complement(<1..2666)
                     /locus_tag="VHS06_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX2988790.1"
                     /translation="MKGLRSGPILFGLTLGIVMLCMLLTMVHASSSPADIAPTSGQAL
                     TPYADQVTPTQGSVPTSADGNDWPQLGHDAQKSNASPQSVDGPYRFYWRWTDVPFASR
                     VQPVVAGGRLFVGGLNGTFYALDAAYDAEGGAPRVLWQRDLGSPIRSGAAVDGSTVIV
                     GTQHGAIYGLDVSNGQLLWSVSTGGAILAAPLVADTTAYLGSADGSFYALRTSDGSVV
                     WKQSIGAPVLASAALSSDGSRLFFMAENIRVYALSISSGAILWQTSQLSGQSAADRWP
                     VVLGNLVVFRTQPLRVWGELLGDGDAVMDSAGARLSDWGADWARVKPKIIAAIDSDPS
                     RQTLFALDTATGQTKGTAPVLHTFGTEDPASPPAVGNGALYLPYRARHGIQNDSPVAV
                     HVSSKYDADLGRMDPATLDIAGVTASDPFSYQFRLTSDEPGVVSIAGDLLLVDNWERL
                     GGINLSTGRLASLAQVAANSPCWSSLASNDALPPFYEGCPFLGPQVGEGSARSGAVAA
                     AGRIFWHVEASGLAAIGPFDGSSVVQLPAATAEPTQSPLPPASQLPASTLASYVWSEP
                     TLPASVSPDLRLRLESEIAKIVTTGNHLMPFYLQKGNHGIGSYPPDVTNDAKPAQVAD
                     SSAFWHDPGELIYSLAITYPYLNPTLQSQLKSYLQSEMNRFPPLSNLPWPTDSWLKQG
                     IARESYQVPMRNSLGTWPPPATPIQTIYALWAYGHYTGDWTYVSSHWSQIQDLFYSKK
                     DQIASYAELSGPIGYARIAKQLGHTAEATTGETMAVSAMQTGYNFQTWLDTANSLYHL
                     TGHYATVEKPGRRAQVFFGLTPEVGRYLHDTNLAAVQQTLNDVVGYPNGSYIWYATRL
                     GTQAERDETSYHTPEIGWSVFMSQAYVNQASQ"
     gene            3434..4699
                     /locus_tag="VHS06_11545"
     CDS             3434..4699
                     /locus_tag="VHS06_11545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2988791.1"
                     /translation="MKILLLTQVVPYPPDSGPKIKTYNVIRYLAQHHEIHLVSFSRSE
                     SETSAAEALRPYCKEITTIPLRRSSLKDAWFLGRSLISGRPFLIERDDSSVMRTAVET
                     QLGRQSFDAVHADQLSMAQFAVGLPLALRVLDEHNAVWTIVHRAAKAGGFGLRRMLAE
                     IEWRKLQAYEADICQRFDFTTVVSIDDFLAIAGPARAIFPARVIPIAVDSQEMAFEPR
                     SAEARHIVSVATMFYPPNAEGVHWFACEVFPLIRQSIPNVQFYIIGARPPDKIVRLAR
                     TGNGVVVTGYLSDLAPTLRKAAVMVVPVHSGSGMRVKILESFSRGIPVVSTTVGAEGI
                     DARNGQHLLVADSPSEIAEAVIRVLTDPLESTKLASAGRELVEAQYDWRTALCGLDEL
                     YPSSISGLPPVPTPADADGPNTLVQAPPR"
     gene            4699..5088
                     /locus_tag="VHS06_11550"
     CDS             4699..5088
                     /locus_tag="VHS06_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988792.1"
                     /translation="MGDFLYRAEPWIITLVLHNWPVMIYLVLIVWSALNALLRPSRPA
                     LLLLYGLVILAVTWEYQKHGIRPIGGTIDYLLLEVNPDLHSVLQQAILYVLPVVANLI
                     GLGLLVTSLLLSIRHRLKTRGAIDSNR"
     gene            5100..6341
                     /locus_tag="VHS06_11555"
     CDS             5100..6341
                     /locus_tag="VHS06_11555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2988793.1"
                     /translation="MRILFVTPYLPSLVRVRPYQFVRELSRRHEITLVATDSNADGKD
                     ADGLRGICRELCVVPMPLTESLRGCLAAAMRGDPLQSAFCSSAELERRLTSILAEKSF
                     DLVHIEHLRAARLVSLVPQEIPTVFDSVDCISLLLERTLRASHSLFQRFLAVLELGRC
                     RRYESRILHRFDRVAVTSPEDKCALNRLAPGAEVAVVPNGVDLEYFRPLEGPREPATL
                     VFSGKMSYHANVSALVHFMDQIFPLIQRARPDVRLRVVGSRPPSLVTRMARNPAVTVT
                     GYLPDIREAVGRATVAVCPMTVKVGIQNKVLEAMAMGLPVVCTRQGATGLQSEADRDL
                     LVARSPAAFAESVCRLLDEPDLRARVGDAGRRYVERHHRWEDAGRRLETLYQEAKTAR
                     EPALMGEAREDIRRRAVSAEY"
     gene            6407..7144
                     /locus_tag="VHS06_11560"
     CDS             6407..7144
                     /locus_tag="VHS06_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2988794.1"
                     /translation="MIGEKALGWGIAEYLNGVAGLLGALPADSLARTINRLEEARCKQ
                     QLVFTCGNGGSATTAIHFASDLAKGAATPNRPGFRSMSLCDNIAMVTAWANDSSYADI
                     FSRRLTTWASRGDVLVAISGSGRSPNVLDAIRVARAAGCTTIGFTGFEGGRMKDMVDI
                     CVTVPSDSMEQIEDVHLVLCHLITKCLRALPLETALHPGPNGSNVIAVAQDVVWPPAD
                     VEEAAPEHGNWHLSLPSLAVGEQPERV"
     gene            7174..8235
                     /locus_tag="VHS06_11565"
     CDS             7174..8235
                     /locus_tag="VHS06_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEX2988795.1"
                     /translation="MSTARDCADFELIARAPLRISLAGGGTDLPAYYERYGGMVVSTT
                     IDKFVYVHCSLNGRGQDHNAQINSADYHTFYRHHCGTPMGWEGDLALPRAALSFFGIE
                     RDISLFLASEVPPGTGLGSSSAVAVALVGAISAFLRRPLSRQQVAEMACEVELGKLLA
                     PIGKQDQFASAYGGLNAITFSGDGVSVEPIGLTSNLLRQLERRLLLFFTGTARSSASI
                     LRDQQRASAQGEARTVEGLHRIKEFAAVCRRRLEVGDLDGIGELLDEGWRQKRGLIGG
                     ITNEWIDQIYDVAIANGAIGGKITGAGGGGFLLLYCHESKQEPLTDSMERLGLRRMDF
                     HFEPQGMTVASVQWAAKRS"
     gene            8253..9251
                     /gene="galE"
                     /locus_tag="VHS06_11570"
     CDS             8253..9251
                     /gene="galE"
                     /locus_tag="VHS06_11570"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX2988796.1"
                     /translation="MKILVTGGAGYIGSIVSERLIQMGERVTVFDNLRTGHREAVPAA
                     CSFVPGDLSNAAQVEGLFAHGGFDAVMHFASNTLVGESMEQPFLYLGDNVVNALNLIR
                     SAARNGVRRFVLSSTANLFDQPLRIPISEDERIVPGSPYGESKYLIERILYWADRVLG
                     MRYACLRYFNAAGAVPGGQRGEDHTPEFHLIPNVLKVALGQRDRVEVFGSDYPTPDGS
                     CVRDYIHVLDLAEAHILSLGALDSGSRHYNLGNGRGFSVWQVIETAREITGHPIPAVV
                     GPRRAGDPSILIADSERIRTELGWVPKHADLHDIMLTAWDWHRTHPAGYVGAGFDT"
     gene            9604..9915
                     /locus_tag="VHS06_11575"
     CDS             9604..9915
                     /locus_tag="VHS06_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988797.1"
                     /translation="MCCFFAILATIVPRLALLLLWVATPLVIRAFDSFLVPLIGLIFL
                     PFTTIAYVLVYSPVYGVSVFGWFLIPNGMPDAPSTTKVAKSSQGGAPLSVPRGCEDAV
                     L"
     gene            10011..10658
                     /locus_tag="VHS06_11580"
     CDS             10011..10658
                     /locus_tag="VHS06_11580"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEX2988798.1"
                     /translation="MRYTVTALIGAFLGVAALLLVQDIRDVICHTPLVGSIICSPAPT
                     VSVASPQPTTTPTPPSIDWKVAGMVHKTIYSEKEYSARFEEKRDYVVIAAKALLQKTI
                     VAVTSGSCSAGIDYEAKPPQAEVAGTRVTVRLPEPAILDCSTRSLEYVDGNWILPAPT
                     DLYNTLADRAINEISTRAEQSDLVAKARESAAIQTELYLRRLGFQQVSIEFLPDR"
     gene            complement(10766..11461)
                     /locus_tag="VHS06_11585"
     CDS             complement(10766..11461)
                     /locus_tag="VHS06_11585"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX2988799.1"
                     /translation="MALENPALNAASFQKGAAEAAEAQRRGMTATGTYARTAFLLLLL
                     LVAAAFGWSQVEIVSVGGRQVVVQPGWTWLAIVLTLVFGMAGAFAYKSAGIFGPLYVL
                     SEGALLGVASHYFNIEYQGIVLQAVLATVCIFGAMLFLYGARIIRVTRGFVLGVAGAM
                     GALLLLWFTAWLISLFGVNFAFLYQPTPLGILLSLGIVILGALNLPIDFNFIENASSS
                     GAPRYMEWYGPLG"
     gene            complement(11485..11772)
                     /locus_tag="VHS06_11590"
     CDS             complement(11485..11772)
                     /locus_tag="VHS06_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988800.1"
                     /translation="MCCFFAILATIVPRLALLLLWVATPLVVRAFDSFLVPLIGLIFL
                     PFTTVVYVLVYSPVYGVSVFGWFLVILALFIDLGSWGGGYAARNRTPNRSL"
BASE COUNT         2387 a         3454 c         3740 g         2423 t
ORIGIN      
        1 tgctggcttg cctggttgac gtaagcctga gacatgaaga ctgaccagcc gatctcgggg
       61 gtgtggtagc ttgtttcatc tcgctcggcc tgcgttccaa gcctggtcgc gtaccagatg
      121 tagcttccgt tgggataccc caccacgtca ttcagagtct gctgtacggc ggccagattg
      181 gtatcgtgga gatagcggcc gacttcgggc gtgaggccga agaagacttg cgccctgcga
      241 cccggcttct ctaccgtggc gtagtgtccg gtcaggtggt acagactgtt ggccgtgtcc
      301 aaccacgtct ggaagttgta gccggtctgc attgctgaca cagccatggt ttctcccgtc
      361 gtagcttcgg ctgtatgccc aagttgtttg gcgatgcgcg cgtatcctat tgggcctgag
      421 agttcggcgt agctggcaat ctgatccttc tttgagtaga agagatcctg gatctggctc
      481 cagtgggaac tgacataggt ccagtcgccg gtgtaatgtc cgtaggccca gagggcgtag
      541 atggtctgga tgggcgttgc cggtggcggc cacgtcccca gtgagttgcg catgggaacc
      601 tggtagcttt cacgggcgat gccctgcttc aaccaactgt ccgtgggcca gggtaagttg
      661 ctcaggggtg gaaagcggtt catctcggac tgaagatagc tcttcaattg actttgaaga
      721 gtggggttca ggtaagggta tgtgatggct aaggagtaga tcaactcgcc agggtcgtgc
      781 cagaaggcgc tggagtcggc gacctgagca ggcttggcgt cgttggtgac gtcgggcggg
      841 taggagccga tgccgtggtt gcccttctgg aggtagaacg gcattaggtg attgcctgtg
      901 gtcacgatct tggctatttc gctctctagg cgcaacctca ggtctgggga gacggaggcg
      961 ggcagagtcg gctcggacca gacgtaggaa gccagtgtgg aggccggcag ttgggaggcc
     1021 ggagggagcg ggctctgggt gggctcagcg gtggctgcag gcaattgaac tacgctcgag
     1081 ccgtcgaagg ggccaatggc agcaagacca ctagcctcga cgtgccagaa gatccttccc
     1141 gcggctgcca ctgcgcctga tctggcgctg ccctcgccga cctggggccc gaggaagggg
     1201 caaccctcgt agaatggggg cagggcgtcg ttggatgcaa gagaactcca gcaaggggag
     1261 ttagcggcga cttgtgccag actggccagt ctgcccgtgg agaggttgat ccctcccagt
     1321 cgctcccaat tgtccacgag aagtaggtcg cctgcgatcg atacgacccc tggttcgtcg
     1381 gatgtgagac ggaactggta ggagaacggg tcagaagccg ttactccggc aatatctagc
     1441 gtggcgggat ccatcctgcc tagatcggca tcgtacttgc tgctcacgtg aacggctacg
     1501 ggagaatcgt tctggatgcc atggcgagcc cgatagggta ggtatagcgc accgttgcca
     1561 actgcaggtg gggacgctgg atcctctgta ccaaacgtat ggagaactgg agcagtgcct
     1621 ttcgtctggc cggttgccgt atccagggcg aagagagtct gcctgctggg atcggagtca
     1681 atggcggcaa tgatcttcgg cttgacccgg gcccagtcag caccccagtc ggacaggcga
     1741 gcgccagcgc tgtccataac ggcgtccccg tcacccagta gttctcccca cacacgtagg
     1801 ggttgtgttc ggaacacgac caaattgccc aacactactg gccagcgatc cgcggcgctt
     1861 tggccggaga gctgggaagt ctgccacaga atcgcaccac tggagatgct cagggcgtaa
     1921 acgcgaatgt tctctgccat aaagaagaga cgggagccat cggaagagag ggcggcagat
     1981 gcaaggacgg gtgcccctat ggactgcttc cagacgacgg agccatcgga ggtacgcagc
     2041 gcgtagaaac tgccgtcggc ggagcccagg taggccgtgg tatcagctac tagaggggca
     2101 gccaggatag ctccgcccgt cgagaccgac cagaggagtt gcccattgga cacgtccagc
     2161 ccgtagatgg cgccgtgctg ggttcctacg atcaccgtac ttccgtcgac ggctgcccct
     2221 gaacgaatgg ggctaccaag gtctctctgc cacaggactc gcggggcacc tccctcggca
     2281 tcataggccg catccagagc gtagaaagtg ccgttgagac ctcccacgaa gagccgcccg
     2341 cccgcaacca ccggctgaac ccttgacgca aatgggacgt cggtccaccg ccaatagaag
     2401 cggtaggggc cgtccaccga ctggggtgat gcgttgctct tctgggcgtc gtgcccgagc
     2461 tgcggccagt cgtttccatc agccgacgtc ggcacgctac cctgtgtagg cgtaacctgg
     2521 tcggcgtagg gggtgagggc ctggcccgag gttggagcga tgtccgcggg acttgaggat
     2581 gcatgcacca ttgtcagcag catacacagc atgacgatgc caagtgtgag cccgaaaagg
     2641 atgggaccgg atctcagacc tttcaactct tgtatccccc cttggaactg actcattcca
     2701 cccgctgatc ggatgcgcgg atgtggcatg cgggcgttct tagcggcact ggcccgaacg
     2761 cctttagctc aaccaatgag ttcacctttc gcttcagcca gtggggtgag gggccgacga
     2821 gcttctctat acagggctga ctgtgctgtt ccacagacaa cgacaaggac cgtctgcgta
     2881 aaactccggt ctgatggtgg aacaactctg gtctcttccg agtgcccagt gagttaggct
     2941 ctcgggttcg gtatgaaaga gttgccccgg acgggtaatg cgttgttcac cccacctaat
     3001 tggctcccca gctttacgat gtggctgccg ggggctcggc ctgctattcg attgattccg
     3061 accaattgat gggcctgggt gaccttgagt agtgccaacc caggtgtgaa gacactggga
     3121 caggatgttg ataacatcgc attgtcgaag gtatgccagt aattgctcat gatcataagt
     3181 gatgatgatt atgcgtgtca atattgtgta gtacaaggta gtgcagcatg tggggaccgt
     3241 atagcgcata tagaaagggc agacaagaaa taataggagc aagcaaggtc aaatatcctg
     3301 ggtcagcggt gagtacagtc ctcactgcat gacctctgct gagggaccct ctgagacggc
     3361 gattcgctct tagaccgatc ccgtgtcgag tctgaggatc ggtgaaagtt gctgagaagt
     3421 ggcgaggggg gccatgaaga tcctcctact gacgcaggtc gtaccctatc cgccggacag
     3481 cgggccaaag atcaagacgt acaacgtgat cagatacctg gcgcagcatc atgagatcca
     3541 cctcgtgtcc ttctctcgct ctgagagcga aacctcggcc gccgaggcgt tgcgtcccta
     3601 ctgcaaagag atcacgacga ttccgctccg caggtctagc ctgaaggacg cctggttcct
     3661 gggacgcagc ctcatcagcg gtcgcccctt tctgatcgag agagacgatt catcggtaat
     3721 gagaacggcg gtggagaccc agttgggaag gcaatccttc gacgcggtcc acgccgatca
     3781 gctttccatg gctcagtttg cggtggggct gccgcttgcg cttcgggtgc tcgacgagca
     3841 caacgcggtt tggactatcg ttcaccgggc ggcgaaagcc gggggctttg ggctgcgtcg
     3901 catgctcgcc gaaatcgaat ggcgcaaact gcaggcatat gaagcagaca tctgccagag
     3961 gttcgacttc acgactgtcg tcagcatcga cgattttctt gccatagccg gccccgcgag
     4021 ggccatcttc cccgcgaggg taatcccgat agcagttgac agccaggaga tggcctttga
     4081 gcccaggagt gccgaggcca gacatatcgt cagcgtggcc accatgttct atcccccgaa
     4141 tgctgagggc gtccactggt ttgcatgcga agtcttccct ctgattagac agagcatacc
     4201 gaacgttcag ttctacatta tcggagcgcg gccgccggac aagatcgttc ggctggcgcg
     4261 tacgggaaat ggcgtggtgg tgacgggcta tctgtctgac ctggcaccca cccttcgtaa
     4321 ggcggcagtg atggtggtgc cagtccactc cggcagcgga atgcgggtca aaatcctgga
     4381 gtcgttctct aggggcatcc ccgttgtctc aacgactgtc ggcgcggagg gcatcgacgc
     4441 tagaaatggg cagcatctgc tggttgcgga ctccccttcc gaaatcgccg aagcggtaat
     4501 acgggtgctc acggatccgc tggagtcaac gaagctcgcc agcgcaggac gagaacttgt
     4561 cgaggcccag tacgattggc gaacggccct gtgtggactg gatgagctct acccatcatc
     4621 gatttccggc ctgccaccgg ttccaactcc ggcggatgcc gatggcccaa atacgctggt
     4681 tcaagcgccc cctcgctgat gggggatttc ctctaccgtg ccgaaccctg gatcattacc
     4741 ctggttcttc acaactggcc ggtaatgatc tacctcgttc tcatcgtctg gtcggcgctg
     4801 aatgccctct tgcggccgag ccggcctgct ctcctgctcc tctatgggct ggtgatcctg
     4861 gccgtgacgt gggagtacca gaagcacggc atacgaccga tcggggggac gattgactac
     4921 ctgcttctgg aggtgaaccc agacctccat tcggtacttc agcaggcgat cttgtacgtt
     4981 ctccccgtcg tggccaacct gatcggcctg ggactgttgg ttacttctct tctgctttcc
     5041 attcgacacc gattgaagac caggggcgcc atcgactcga accgatagcg aggcatggca
     5101 tgagaatcct gtttgtcacc ccctaccttc catccctggt tcgcgtacgt ccctatcagt
     5161 ttgtgagaga gctgtctcgc agacacgaga tcacgctggt agcgacggac tcgaatgccg
     5221 acgggaagga tgcggatggc ctcaggggga tttgccgaga gctttgcgtg gtgccgatgc
     5281 ccctcacgga gagcctgcgt ggctgtctcg cagcggcgat gcgaggggat ccgcttcagt
     5341 cggcgttctg cagttcggcc gagctcgagc gccgcttgac gagcattctg gccgagaaga
     5401 gctttgattt ggtgcatatc gagcaccttc gggcggcgcg tctggtttcc cttgtcccgc
     5461 aggagatccc gacggtgttc gattcggtcg actgcatatc gctcctcctg gagcgaactc
     5521 ttagggccag ccacagcctg tttcagcgct tcttggccgt actggaactg ggcagatgcc
     5581 gtcgctacga atcgcgcatt ctgcatcgct tcgatcgcgt cgccgtaacc tctccggaag
     5641 acaagtgtgc cctgaaccgt ctggctccgg gtgcggaggt ggctgtggtg cccaacgggg
     5701 tagacctaga gtactttcgc ccgctcgagg ggccgcgtga gcccgcgacg ctggtcttct
     5761 cggggaagat gtcgtaccac gccaacgttt cggccctcgt ccacttcatg gaccagatct
     5821 ttccccttat ccagagagcg cggccggacg ttcggctgcg tgttgtggga agccggccgc
     5881 cttctctggt gacaaggatg gcccgaaatc cggcggtgac ggtcacgggc tacctgcccg
     5941 acattcgaga ggccgtggga cgcgccaccg ttgccgtttg ccctatgacc gtgaaggtgg
     6001 gtattcagaa caaagtgctg gaagcgatgg cgatgggctt gccggtggtt tgtaccaggc
     6061 agggggcaac cggtcttcag tcagaggcag atcgtgatct attggtcgct cgcagcccgg
     6121 cagcctttgc ggagtcggtc tgcagattac ttgatgagcc agacctgagg gcgcgagtcg
     6181 gcgatgcagg ccgcagatac gtggagaggc atcaccggtg ggaggacgcg gggcggcgac
     6241 tggagacgct gtaccaggaa gccaagaccg cgcgggaacc ggcgctgatg ggcgaagctc
     6301 gcgaggatat ccgacgccga gcggtgagcg cggagtactg agcgactctc tcatgttggc
     6361 gtcgacgaat ggtggggtta gaccggctta ttcgggaggg gctaccgtga ttggagagaa
     6421 ggctctcggt tggggcattg ccgaatatct gaacggtgtt gccggcctgc ttggcgcatt
     6481 gccggccgac tccctggcga ggacgatcaa ccgtcttgaa gaggcgaggt gcaagcagca
     6541 gttggtgttc acctgcggaa atggtgggtc tgccactacc gcgatccact tcgcctctga
     6601 tctggcaaag ggcgcagcaa cgccaaaccg gcccggcttc aggtcgatgt cgttgtgcga
     6661 caacatcgcc atggtaacgg catgggccaa cgattcttcg tacgccgaca tcttctcgcg
     6721 tcgcctgaca acgtgggcat cgcgagggga cgtgttggtt gccatcagtg gaagcggcag
     6781 atctcccaat gtgctcgatg ctatacgggt agctcgcgcg gcaggatgca ccactattgg
     6841 cttcaccggc ttcgagggag ggcggatgaa ggatatggtg gacatctgcg tgacggtgcc
     6901 cagcgacagt atggaacaga tagaagacgt tcacctcgtc ctgtgccatc tcataacgaa
     6961 gtgccttcgc gctcttcctc ttgagactgc cctgcatccg ggtccgaacg gctcaaatgt
     7021 gattgcagtt gcccaggacg tggtgtggcc gccggctgat gtggaagagg ccgctcccga
     7081 acatgggaat tggcacttgt ccctgccgtc gctcgcggtg ggagagcagc cggaacgggt
     7141 gtgaggcgag actcaaggtt gtgaggtatc gcgatgtcaa cagcgcgaga ctgtgcagac
     7201 ttcgagctta ttgccagggc tcccctgagg atcagtctcg ccgggggcgg gacggacttg
     7261 ccggcgtact atgagcgata cggtggaatg gtggtttcga cgacgatcga caagttcgtc
     7321 tatgtccact gtagcctcaa tggacgtggg caagaccaca atgcccagat caactccgcg
     7381 gactaccaca ccttctaccg gcatcattgc ggaaccccca tgggatggga gggcgacctc
     7441 gcgctcccgc gggcagcact cagcttcttc ggtattgagc gagacatatc gctctttcta
     7501 gcctccgagg tgccacccgg aaccggcctc ggttcctcaa gcgctgtcgc ggtcgccctg
     7561 gtgggagcca tttcggcctt ccttcgccga cctctatcca ggcagcaggt ggcggaaatg
     7621 gcttgtgagg tcgaactggg gaagttgttg gcgcccatcg gcaagcagga tcagttcgcc
     7681 tcggcttatg gcggattgaa cgcgatcacc ttctccggcg atggagtcag tgtcgagcca
     7741 attggcctta ccagtaacct tctgcggcaa ctggaaagac gattgttgct cttcttcacg
     7801 ggcacggcta gatcctcagc ctcgatactc agggatcagc agcgggccag cgctcaaggt
     7861 gaggctcgca cggtcgaggg gctacaccgg atcaaggaat tcgccgccgt gtgcagacgc
     7921 cggctcgagg taggtgacct ggatggcatt ggcgagttgc tcgatgaggg gtggagacag
     7981 aagcgcggcc tgattggcgg catcaccaac gagtggatcg atcagatcta cgacgttgcc
     8041 attgccaacg gagcgatagg tggaaagatc accggcgccg gcggaggggg gttcctgctt
     8101 ctgtactgcc atgagtccaa gcaggaaccg ctgaccgact cgatggaacg gcttgggttg
     8161 cgtcgaatgg atttccactt cgaaccgcag ggcatgacag tcgcctccgt tcaatgggcg
     8221 gccaagcgca gttagctggg gagacaacca gaatgaagat actcgtgacc ggcggtgcgg
     8281 gctacatcgg ttccatcgtc tccgaacgac taattcaaat gggcgagcgg gtgacagtgt
     8341 tcgacaatct gcggaccggc catcgggagg ctgttcctgc cgcctgctca ttcgttcccg
     8401 gcgatctgtc caatgcagcg caggtcgaag gactgttcgc acatggtggt ttcgatgcgg
     8461 tgatgcattt tgcctccaac actctggtcg gtgagtccat ggagcagccg ttcctctatc
     8521 taggggataa cgtagtcaat gctctgaatc tgatccgatc ggctgcaagg aacggcgtga
     8581 gacggttcgt tctctcctcc acggcgaacc tgttcgatca acctctgcga attccgatat
     8641 ccgaggacga gaggatagtg ccgggcagcc cgtatggcga atcgaaatac ctcatcgaaa
     8701 ggattttgta ctgggcggac agggtgttgg gaatgcgcta cgcttgcctg cgctacttca
     8761 acgctgccgg agcagtcccc ggaggccagc gaggtgagga tcacacacct gagttccatt
     8821 tgatccccaa tgtcctgaag gtcgctctgg gacaacgtga tcgtgtggag gtctttggtt
     8881 cagattaccc gacaccggac ggcagttgcg tccgcgacta cattcacgtg ctcgacctgg
     8941 cggaggcgca catactgtcg ctgggggcgc tggattccgg cagtcgccac tacaatctcg
     9001 ggaacgggcg cggcttcagc gtctggcagg tgatcgagac cgcacgcgag atcaccggcc
     9061 atccaatccc ggccgtggtg ggtcctcgga gagcaggcga tccgtcgata ctgatcgccg
     9121 actccgaaag gatccgcact gaactggggt gggtgcccaa acatgccgat ctgcatgaca
     9181 taatgctgac ggcctgggat tggcaccgaa ctcatccagc cggctacgtt ggtgccgggt
     9241 ttgatacctg accttctccc acttctcctt ccctgcctcg ttccccttct tccatcttgg
     9301 cggcacgttg tgccttgaac cgaccttccc cttctaccgc ctatcagtac ccccctcgca
     9361 tcctccctta acccccgttc tatgcctcga ttgcaccacg aagtcgatcc tcagctaatg
     9421 cctctccacc cggtgttgtg gttttctacc ctcgttcaga agataggcgg aacaggcagc
     9481 atgtgtcgaa cgaggcgtgt gtctctgcca tgcaaggagg gtacaaggat tgcacatcct
     9541 cgacggacag cgccggtgcc cagcggatga gcgggtcaca atccggtgcg ggagggaccg
     9601 cgcatgtgtt gtttcttcgc catcctggcc accattgttc ctcgattggc gctactgctg
     9661 ctttgggtcg cgacccctct ggtgatcagg gctttcgact cgtttctcgt gccgctgata
     9721 ggcttgatct tcctgccctt caccaccatc gcatacgtcc tcgtctatag ccctgtgtac
     9781 ggagtctccg tatttgggtg gttcctgatc cccaatggca tgccagatgc accatccaca
     9841 acgaaggtgg cgaagtcctc acagggcggt gcgccgctgt cggtgccgag aggatgcgaa
     9901 gatgcggttc tctgactccc cggcccacgc cggcgccgat ggtaatccgc gtcgaccttg
     9961 atgaggccaa aatagtgcac gggacctttg gccgtcggag ggaggcacga atgcgataca
    10021 ccgtcacggc cctgatcggt gcctttcttg gagtggcggc gctcctgctg gttcaggaca
    10081 ttcgggatgt gatctgccac acgccgctgg tcgggagcat catttgcagt ccggcgccca
    10141 ctgtatcggt cgcctcgcct cagcccacaa ccacacccac acctccttcc atcgattgga
    10201 aggttgcagg gatggttcac aagaccatct actcggagaa ggagtattcg gcgcgattcg
    10261 aagagaagcg agactacgtt gtcatcgcgg cgaaggcact ccttcagaag accatcgtcg
    10321 cggttacttc tggttcgtgc tcggccggca tcgactacga ggcaaagcct ccacaagccg
    10381 aggtggccgg gacccgagtc acggttaggc tcccggaacc cgcaatcctg gactgcagca
    10441 cgcggtcgct cgagtatgtc gacggcaact ggattctccc tgcaccaacc gatctctaca
    10501 acacgctggc cgaccgcgca atcaacgaga taagcacccg agccgaacag agcgacctcg
    10561 tcgccaaagc gcgcgagagc gcggcaatcc agaccgagtt gtacctcagg aggctgggct
    10621 tccagcaggt ctcgatagag ttccttccgg accgatgacg gagtggaatc tgcgaagcgg
    10681 gtgcgcaatg ggcttcaact gctggcggca cggagcagcg ccagaagccg caagatagac
    10741 acgtatatcc atatcagcga gagcgtcagc ccaaaggccc gtaccattcc atatacctgg
    10801 gtgctccaga tgaggatgca ttctctatga agttgaaatc gataggcagg ttgagcgctc
    10861 caaggatgac aatccccagg ctcaagagaa tgcccaacgg ggtaggctgg tacagaaacg
    10921 cgaagttcac tccgaacagg gagatcagcc acgcggtgaa ccagagcaag agcaaggcgc
    10981 ccatggctcc agccactcca aggacaaatc cccgagtgac tctgatgatc ctggcaccgt
    11041 acaggaagag catcgcgcca aagatgcaca ccgtcgcgag aactgcctgg agcactatcc
    11101 cctgatactc gatgttgaag taatgagagg ccacacccag cagggccccc tcgctgagga
    11161 cgtagagggg cccgaagatg ccggccgact tataggcgaa ggctcccgcc attccaaaga
    11221 cgagggtgag cacgatggcc agccaggtcc accccggctg aaccaccacc tgtcggccgc
    11281 cgaccgagac aatctccacc tgggaccagc cgaaagctgc cgccaccaac aacagcagca
    11341 acaggaaagc tgtacgggca tatgttcctg tcgccgtcat gccgcgtcgc tgggcctccg
    11401 cggcctcggc agcccccttc tggaaagagg cggcgttcag ggcagggttc tcgagagcca
    11461 taacctactc ctttaggaca cgatctacag gctccggttt ggggtccggt tgcgagcagc
    11521 gtagccccct ccccaggacc ccaggtcgat gaacagcgcc aggatcacca ggaaccaccc
    11581 aaatacggag accccgtaca cagggctata gacgaggacg tataccaccg tggtgaaggg
    11641 caagaagatc aagcctatca gcggcacgag aaacgagtcg aaagccctga ccaccagagg
    11701 ggtcgcgacc caaagcagca gtagcgccaa tcggggaaca atggtggcca ggatggcgaa
    11761 gaaacaacac atgcgcggcc cctcccgcac cggattgtga cccgctcatc cgctgggcac
    11821 cggcgctgtc cgccgaggat gtgcaatcct tgtaccttcc ttgcatcgca gagacacacg
    11881 cctcgttcga cacatgctgc ctgttccgcc tctcttctga acgagggtag aaaaccacaa
    11941 ccctgggtgg agaggcatta gctgaggatc gacttcgtgg tgcaatcgag gcatagaacg
    12001 gggg
//
LOCUS       DATALS010000517         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_311540, whole genome shotgun sequence.
ACCESSION   DATALS010000517 DATALS010000000
VERSION     DATALS010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311540"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VHS06_11595"
     CDS             complement(<1..541)
                     /locus_tag="VHS06_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2988801.1"
                     /translation="MHIKITTLAENTAAKMGVLAEHGLSMLVEVDDLCLLLDTGQTDT
                     VVRNARVLGVDLSRVQHIVLSHGHSDHTGGLLRVLAETGEVIVHAHQEVFRPRYGTRG
                     GGKPRYTGLPHLREQLESMGARFQLSEGPDRITPQAMTTGPVPRITPFEHVDVDLKVR
                     TPRGLEQDEIPDDQSLLIKT"
     gene            complement(764..>1600)
                     /locus_tag="VHS06_11600"
     CDS             complement(764..>1600)
                     /locus_tag="VHS06_11600"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX2988802.1"
                     /translation="LDQGSLVRALEHTKPDEVYNLAAISFVPLSWNQGELTGDITGLG
                     VLRMLEAIRIVGGSGRRNGVRINPIRFYQASSSEMFGKVQETPQSERTPFYPRSPYGV
                     SKVYGHYITVNYRESYDIYACSGILFNHESPRRGFEFVTRKITHAAARIKLGRQKELL
                     LGNLDSKRDWGFAGDYVKAMHMMLQQTEPDDYVVGTGEFHSVAEFAEIAFARVGLDWR
                     DYVRTDARFMRPAEVDHLLANPTKAHTKLGWRPSVSFHQLVEMMVDGDMELEQQKIAR
                     GM"
BASE COUNT          254 a          549 c          500 g          297 t
ORIGIN      
        1 gcgtcttgat cagcagcgac tggtcgtccg ggatctcgtc ctgctccagc ccgcggggag
       61 tccgcacctt caggtccacg tccacatgct cgaaaggggt gatccgaggg acgggcccgg
      121 tggtcattgc ctgcggggta atccggtccg ggccctcgga gagctggaag cgggccccca
      181 tcgactcgag ctgctcccgc aggtggggca ggccggtgta gcggggcttg ccgccccccc
      241 tcgtgccgta gcggggccgg aacacctcct ggtgggcgtg gacgatcacc tcgcccgtct
      301 ccgccagcac ccgcaacagc ccgccggtat gatccgagtg gccgtggctg agcacgatgt
      361 gctgaaccct ggacaggtca acccccagca cccgcgcgtt gcgaaccacg gtgtcggtct
      421 ggccggtgtc cagcaagagg cacaggtcgt ccacctccac cagcatcgag agtccgtgct
      481 cggccagtac acccatcttg gctgcggtgt tctccgcgag ggtcgtgatc ttgatgtgca
      541 cggctagacc tcctcaggaa gaaagaacga agcccttccg atccgctggc cagcggcccg
      601 aagggcttcg cccaatcatc tcgtattccg ctgctcgggc agcctcaacc gctaccggcc
      661 gggtgagggc tctctgcgtc tctgtgcccc tgcgggcctc cggcccgcct gcggcgctga
      721 cgcgccgcac tccatatctc cgtgtctccg cgcccccgtg tcgctacatg ccccgcgcga
      781 tcttctgctg ctccaactcc atgtcgccgt cgaccatcat ctccaccagc tggtggaagg
      841 agaccgaggg ccgccagccc agcttggtgt gcgccttggt cgggttcgcc agcaggtgat
      901 ccacctcggc cggccgcatg aagcgagcgt cggtccggac gtagtcacgc caatccaatc
      961 ccacccgtgc gaaggcaatc tcggcgaact ctgccacgct gtggaactcg ccggtgccca
     1021 ccacgtaatc gtccggctcg gtctgctgca acatcatgtg catcgccttg acgtagtctc
     1081 cggcgaagcc ccagtcccgc ttggagtcca ggttgcccag cagcagctcc ttctgcctgc
     1141 ccagcttgat cctggcggca gcgtgagtga tcttcctcgt tacgaactcg aacccgcggc
     1201 ggggggactc gtggttgaag aggatgccgc tgcaggcgta gatgtcgtag ctctcacgat
     1261 agttcaccgt gatgtagtgg ccgtacacct tggagacacc gtaggggctg cgagggtaga
     1321 agggggtcct ctcactctgg ggcgtctcct gcaccttgcc gaacatctcg ctggagctgg
     1381 cctggtagaa gcggatcgga ttgatcctca cgccgttgcg ccgtccgctg ccgccgacga
     1441 tgcggatagc ctccagcatc cgcaatacgc cgaggccggt gatgtcccct gtcaactctc
     1501 cctgattcca ggaaagggga acgaaggaga tggcggccag gttgtacacc tcatccggct
     1561 tggtgtgctc cagagccctg acgagggagc cctgatccag
//
LOCUS       DATALS010000518         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_312329, whole genome shotgun sequence.
ACCESSION   DATALS010000518 DATALS010000000
VERSION     DATALS010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312329"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2047)
                     /locus_tag="VHS06_11605"
     CDS             complement(<1..2047)
                     /locus_tag="VHS06_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2988803.1"
                     /translation="MKERTAKDYTIWLAALCLALLTLVTYWQTFGHDYVAYDDEQYVT
                     MNPYVQQGLTRDSIRWAFTSYHASNWHPLTWMSHMLDQRLFGMSPVGPHAVNLLLHIA
                     NTILLLFVLRRMTKSLWKSAFVAALFALHPMHVESVAWVAERKDVLSTLFLLLTVWAY
                     VKYTERPSTSRYLPVMLLFGLGLLSKPSLVTLPFALLLLDYWPLRRLQTADKRQQKGL
                     VGLIAEKIPLIVMAVASSAATYWAQNKGGAMIVGDDRLSLPIRMDNAIVSYANYIGRM
                     FWPHDMAIFYPHPASHIPVWQVAAGLVLLAGITVLALKLGRRHPSLPVGWLWYLGILV
                     PMIGLVQVGKAAMADRYTYVSYIGLFIMIAWGIPECMGVREHGSMGGENSHTPTRPHS
                     HTALSAVAGLVIAALCVCTWFQLRYWQNAMTLFSHALAVTSGNDGAHIQVGNYLFKEG
                     KTVEAIKHYQAALKINPYTDRAHTNLGNAYARLGQTDLAIREYQAALELRQNPVAYYN
                     LGNLYSRQGKLEEAVGFYKKALDIQPDYVEARTNLSRTLVKLGRTDEAAEQASELAKL
                     MPNSAQSHYQLALVLMQAKNLDGAIAEYRAAIRLKSDFAPAHYNLGTVLAQKGQLDEA
                     FQEFSEAVRIKPDYADAYFNIGVVYDSRNDMALAMKEYRLAIKVKPDHAEAHTNLAID
                     "
BASE COUNT          404 a          612 c          649 g          414 t
ORIGIN      
        1 ggtctatagc gagattggtg tgcgcctccg catggtccgg cttgaccttg atcgcgaggc
       61 ggtactcctt catcgccagg gccatgtcat tgcggctgtc gtacacaacg cctatgttga
      121 agtatgcatc ggcgtagtca ggcttgatcc gcaccgcctc cgagaactcc tggaacgcct
      181 cgtcaagctg acccttctgc gcgagcacgg tgccaaggtt gtaatgggcc ggcgcgaagt
      241 cggacttgag ccgtatcgcc gctctgtact cggcgatagc accgtcaagg ttcttcgcct
      301 gcatcaggac caacgccaac tggtagtgcg actgcgcgga gttcggcatc agcttcgcta
      361 gttcggatgc ctgctcggca gcctcatcgg tgcgtcccag cttgaccagg gtccttgaca
      421 ggttcgtccg agcctccacg tagtcaggct gaatgtcgag cgctttcttg tagaagccta
      481 ccgcctcttc gagcttgccc tggcgcgagt agagattgcc gagattgtag taagccaccg
      541 gattctggcg cagctcgagc gccgcctggt actcgcggat ggcgagatcg gtctggccta
      601 ggcgcgcata tgcgttaccg agattcgtat gtgcccggtc ggtgtacgga ttgatcttca
      661 acgccgcctg gtagtgcttg atggcttcca cggtcttccc ctctttgaag agatagttcc
      721 cgacctggat atgcgcgcca tcgtttccag acgtgaccgc gagcgcgtgg ctgaacaagg
      781 tcatggcgtt ctgccagtag cggagttgga accacgtgca gacgcagaga gcggctatca
      841 ccaagcctgc gactgccgac aacgcggtgt gggagtgtgg gcgtgtggga gtgtgggagt
      901 tctctccccc catgctccca tgctcccgta ctcccataca ctccggtatc ccccatgcaa
      961 tcatgatgaa gagcccgatg taggagacgt aggtgtatct gtccgccatg gcggccttgc
     1021 cgacctgcac gaggccgatc atgggcacga ggatgcccag gtaccagagc catcccaccg
     1081 gcaaggatgg atgcctacga ccaagcttga gggccaggac cgtgatgccc gcaagcagga
     1141 cgaggcccgc cgcgacctgc cacacgggaa tgtggctcgc gggatgcgga tagaagatcg
     1201 ccatgtcgtg aggccagaac atccggccga tgtagttcgc atacgagaca atcgcgttgt
     1261 ccatgcggat gggcagggac aggcggtcgt cacctacgat catcgcgccg cccttgttct
     1321 gcgcccagta ggtcgccgcg ctggatgcaa ccgccatcac gatgagtgga atcttctcgg
     1381 cgataagccc aaccagtccc ttctgttgtc tcttgtctgc agtctgcagt ctacgaaggg
     1441 gccagtagtc gagaaggagg agtgcgaacg gcagcgtcac cagcgacggc ttcgacagga
     1501 gtcccaggcc gaagaggagc atcaccggca gataccggct tgtactcgga cgctccgtgt
     1561 acttcacgta ggcccacacg gtcaggagca ggaacaaggt gctcaggacg tccttgcgct
     1621 cggcaaccca cgcgacggac tccacgtgca tcggatgcag ggcgaacaag gccgcgacga
     1681 atgcgctctt ccacagcgac ttcgtcattc ggcgcaggac aaacagtagg aggatggtgt
     1741 tcgctatgtg gagcaacagg ttgaccgcgt gcgggccgac cgggctcatc ccgaaaaggc
     1801 gctgatcgag catatgcgac atccaggtca gtggatgcca gttgctcgcg tggtatgacg
     1861 tgaacgccca tcggatggag tccctggtca ggccctgctg gacgtacggg ttcatggtca
     1921 catactgctc gtcgtcatag gcgacgtagt cgtgcccgaa cgtctgccag tatgtaacca
     1981 gggtcaacag cgcgaggcag agggcggcca gccagatcgt gtagtccttc gcggttcgtt
     2041 ccttcaagga gtcttcctgc gaggaaaagc gtcagacga
//
LOCUS       DATALS010000519         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_447720, whole genome shotgun sequence.
ACCESSION   DATALS010000519 DATALS010000000
VERSION     DATALS010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447720"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VHS06_11610"
     CDS             <1..803
                     /locus_tag="VHS06_11610"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX2988804.1"
                     /translation="VWAGRNQRFTPAQEEVLSYFASEVAAAVKNVVMLTETLSHASAV
                     ESKILEMSLWQRQLNEYRDDLARTHEELTQTQSVLEETYMATLEALAGAVDARDPYTY
                     GHCKRVAGIASSLALQMGLPDLERGSLELAALLHDIGKIGVPDNVLRKVTPLSQEDAA
                     AIHQHPEMGYRMLSALRFLGSGLAAVRYHHERFDGSGYPYALSGNQIPLLARILAVAD
                     AFDAITSDRPYRRARSRDQGVEEVRRCAGSQFDPSVVEAFVAFMNTAA"
     gene            complement(865..2454)
                     /locus_tag="VHS06_11615"
     CDS             complement(865..2454)
                     /locus_tag="VHS06_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988805.1"
                     /translation="MARIAGSTLDREELLQALFVELNRSVPCDSLGFYWREPGESFCL
                     VVVRPGLTAIEVEQTQVSGEADIVDDKPQPYCCQENSSSNHPVLRWLAAHDLSSSLNV
                     PVIVAQQLLGYFVASRTGSHTFAVADLHPLVQIAGMVGPSLNSMQLYERLELAHQNLK
                     ATRDELARSERLMVQGELASGVAHDINNILGLISARTDLLKLQGLPQQAAASAEAISQ
                     AVDDGITVVRRMTQFTRRQRKQEVVPLDVNKLVEGVMEMTRPRWQSASSLNKVSVRFT
                     PGPVSTVLGVPSELREVLVNLVMNSVDAMPSGGWIRIDVEQADGYAYISVADTGCGML
                     EEVREHIFDPFFTTKGESGSGLGLWVSHGIIVRHGGDIEVHSEVGKGSRFRVRLPLGR
                     AVQEQAPKRVECVEYSILVVDDEAGLGEALKLSLEMAGYHVLFSANPRQALELFKQEQ
                     FDLVITDLRMPGMSGWELASEIKRFNPSTPIIAMTGWPVDLIRDGQRCSEMEAIIQKP
                     YRVNDLRAKVARILAERKEKP"
BASE COUNT          607 a          906 c          765 g          529 t
ORIGIN      
        1 aggtgtgggc agggcgaaat cagcgcttca cgccggccca ggaagaggtt ctcagctact
       61 tcgcatcaga ggttgcagca gcggtcaaga acgtagtgat gctgactgaa accctcagcc
      121 atgccagcgc cgtcgagtcc aagatcctcg agatgtctct ctggcagagg caattgaacg
      181 agtaccgtga tgacctagcg cgaacccacg aggagctcac tcagacacag tccgttctgg
      241 aggaaaccta catggcaacc ctggaggctc tggcaggtgc cgtcgatgct cgtgatcctt
      301 acacctacgg acactgcaaa cgcgtcgcag gaatagcatc ctcgctcgcc ctccagatgg
      361 ggctccctga tctggagaga ggcagtctgg agctggccgc cctcctgcac gatatcggca
      421 aaatcggcgt ccccgacaac gtccttcgta aggtcactcc gctgtctcag gaggatgcag
      481 cggcaatcca tcagcatccg gagatggggt acaggatgct gtccgcgctc cggttcctcg
      541 ggagtgggct tgcggcggtc cggtaccacc atgagaggtt cgacgggtcc ggctacccct
      601 acgcgctatc gggcaaccag atacccctgc tagccaggat cctggcggtc gcggatgcct
      661 tcgacgccat cacgtcggac cgcccatatc ggcgcgcacg atcccgagac cagggggtcg
      721 aggaggtgag aaggtgcgcc ggcagccaat tcgacccctc ggtggtagag gcattcgtcg
      781 catttatgaa cacggctgcc tgacgctgtt ggtgtatcga cgaaagggaa tggcaaaccg
      841 gaaagcgacg cttcccccag gctcttacgg cttttccttg cgctcggcca ggatccgagc
      901 caccttagcc ctcaggtcat tcacccgata gggcttctgg atgatggcct ccatctccga
      961 gcatcgctgc ccatcccgaa tcaggtctac cggccatccg gtcatggcga ttatgggggt
     1021 agagggattg aatctcttga tctccgaagc cagttcccag ccggacatcc ccggcatccg
     1081 gaggtcggtg atcacgagat cgaactgctc ctgcttgaag agctccaacg cctgccgcgg
     1141 attggccgag aagagcacat gataccctgc catctccagc gacagcttca gtgcttcacc
     1201 gagaccggcc tcgtcgtcca ccacaaggat agaatactcg acacactcca ccctcttcgg
     1261 tgcctgctct tgaaccgccc tgcccaacgg cagcctcacc ctgaaccggc ttcccttgcc
     1321 gacctcgctg tgcacttcaa tgtctccgcc atggcgcaca attatcccgt gggataccca
     1381 gaggcctaaa ccagaacccg actcgccctt cgtggtgaag aagggatcga agatgtgctc
     1441 ccgcacctcc tccaacatac cgcagccggt atcagcaacg ctgatgtatg cataaccatc
     1501 ggcctgctcg acatcgatgc gaatccaacc tcccgagggc atcgcatcga ccgagttcat
     1561 caccaggttg accaaaacct ccctcaattc tgagggcaca cctagcaccg tggagactgg
     1621 cccgggggtg aaacgcaccg acaccttatt caagctcgaa gcagactgcc agcgtggacg
     1681 ggtcatctcc atcacgccct ccaccagctt gttcacatcg aggggaacaa cctcttgctt
     1741 acgctggcgg cgggtgaact gggtcatgcg gcgcaccacc gtgatgccat cgtccactgc
     1801 ctgactgata gcctcggccg acgccgctgc ctgctgtggc aggccctgaa gctttaggag
     1861 atcggtcctg gcggagatca acccaagaat gttgtttatg tcgtgcgcaa cgcccgacgc
     1921 taactcaccc tgtaccatca accgctcgga ccttgccagt tcgtcacgcg tggccttcag
     1981 gttctggtgt gccaactcca accgctcata cagttgcatt gagttcagtg aaggtccaac
     2041 cattccggcg atctgaacca acgggtgaag atccgcaacg gcaaaggtgt ggctaccagt
     2101 tcggctggcg acaaagtacc ccagcaactg ttgcgctacg atgactggga cattgaggct
     2161 cgaagagagg tcgtgagcgg ccaaccatcg caacaccggg tggttgctgg aggagttctc
     2221 ctggcagcag tacggttgag gcttgtcatc gactatgtcc gcttctccgg atacctgggt
     2281 ctgctcaacc tctattgcgg ttaagcccgg gcgcacgaca accaggcaaa agctctcgcc
     2341 cggttcccgc caatagaacc ccagcgagtc acacgggacg cttcgattca actcgacgaa
     2401 cagcgcttgg agtagttcct cccgatccag ggtggacccc gcaatccttg ccaccctctc
     2461 cagcgcggaa tcaccccaac ccaacaactc tgtgcggcca gaggatctat atgcagtcaa
     2521 gatccgtcaa cctccacaca aagacgcgac tggagcgcag cttgctcgga tcgattaccc
     2581 cggtcgtcga gcataccccg actggttgcg caccttcaac aacaaagcga cagcaacact
     2641 tcaccactgc gggatagttc gcgcttgcac gtttcgagaa aggaggactg gggtagaagc
     2701 aacaaccgag atcacacgaa cgggaactgc agcccccgca tctatcggta cgcctgagag
     2761 gattcgaacc tccgacccgc ggcttagaag gccgccgctc tatccac
//
LOCUS       DATALS010000520         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_313021, whole genome shotgun sequence.
ACCESSION   DATALS010000520 DATALS010000000
VERSION     DATALS010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313021"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..374
                     /locus_tag="VHS06_11620"
     CDS             135..374
                     /locus_tag="VHS06_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988806.1"
                     /translation="MTRTVDAEDREVAARLAVIESKLEAVLGELSIVRNYIPSKMVEH
                     SERIAVLERNLRTIQWVGSVFAAAIIGTFLAHILR"
     gene            708..2693
                     /locus_tag="VHS06_11625"
     CDS             708..2693
                     /locus_tag="VHS06_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988807.1"
                     /translation="MSVLASTACPAAIGVFRWYATPDGATAGISYYLNLAGDSGRIEI
                     CPDQSGSPGSPIRTIALTPTDLSPGSHTVTWDGNEDDGSPAPAGSCLARIYVHAAPIP
                     SPTNPNDPLQMPQNRKMLTTPSGNFYGVAADCSPESNHQLMPSSSSYGFIYAPNTLSK
                     KMDVYYPDGVYKNSWSTALSGTKAPWGVSVDGEGRVYSASQNSWVVYNFASDGSNMLG
                     GNICGVLYGGGAAWGKAADGTARFQTVVSNKLTEWRILPEAVMLNPPDTTKWTNFDST
                     GKIEVLLPMTGCTQTARDTVNGFLYVTQYNATSQVSLRRFQWTGSGYTIDDTWLPEVG
                     SNVCGVAVCAVDPNILFVTRQSGVTNLERWYVEHPSPSISGPALLDAYKVADIVVHNV
                     ASDSRGNALFDAGGSDRNSVQRSIGMFIFPDGGSEDTRLAGPFYHDGPANTLALEHVR
                     DVATVPAGSSVSLEGKIVSAKFGSVLYVQEEDRSAGVRVEPASQVEAQPGTSVKVVGT
                     VDCSLGERRITDAQVTPGITGEAPKPLMMGTRFADNDPSEQLEFVGLNNLGLLVRVAG
                     RVTAVDSANSLFYVDDGQGLDNGFGSRGIAVSFSGMGPDVRQEDVVSATGISSCRKLV
                     LSESRTINGHEANSGTTVYEPTILLRGVSDVFVIKRP"
BASE COUNT          600 a          744 c          833 g          526 t
ORIGIN      
        1 gacgatacta tgtccagaaa tggaagaaca tatacagtaa ggaagatcgc tgtggagcgg
       61 ctggggaact tgccggtgct tacttttgcg gtgcttagct gactttcgcc ggacttaccg
      121 gaggtgggtg cacaatgaca cgtacagttg acgcggaaga tcgggaagta gccgccaggc
      181 tggccgtaat cgagagcaag ctggaagccg tgctgggcga actatccatc gtgcggaact
      241 acatcccaag caagatggtg gagcactcgg aacggatagc cgtgctggag aggaacctgc
      301 ggactataca atgggttggg agcgtatttg ccgctgcgat catcggcacg ttcctggcgc
      361 acatcctgag gtagtacaaa agaatacccc gtgggtgaag cggtcaccca cggggtcctt
      421 tcaggaggct gttcagctga tctctctttg gacggggaaa gatccttccg aaaggaaagg
      481 ctttacagcg tatgtgctac ttgtaatacg gctgaattgg ggaaaatgtt cccaatttca
      541 caaatgccct cgctgctaac aaacctgtaa gaattacctg gttttgcgac gtaaacttga
      601 caggaaaagc ctcatgctgc tagagtctgg cagccagcct tatgattggg ggccaagata
      661 tgagtttgcg ttgtcgtgtt acagcagtcg cagttcttat cgcttctatg tccgtattag
      721 cctcgacagc atgccctgcc gcgatcgggg tcttcagatg gtacgccaca ccagatggcg
      781 caaccgcagg aatctcctac tacctgaacc tcgcaggtga ctccggaagg atcgagatat
      841 gcccggacca gtccgggtcg ccgggaagcc ccatcaggac cattgcactt acgccgaccg
      901 atctgagccc gggctcgcac acggtcacct gggacggcaa cgaggacgac gggtctccgg
      961 ctcccgctgg ctcctgtctc gccaggatct atgtgcatgc cgcacccatc ccctcaccca
     1021 ccaaccccaa cgaccctctg cagatgccac agaacaggaa gatgctgacg acaccgtcgg
     1081 ggaatttcta tggggtggca gcagactgca gtcctgagag caaccatcaa ctgatgcctt
     1141 catcctcctc ctatgggttc atctacgcac ctaacaccct aagcaagaag atggacgtgt
     1201 actacccgga tggcgtatat aagaactcat ggtccactgc tctgtcaggc accaaagctc
     1261 cttggggagt atcagttgat ggagaaggaa gagtgtattc ggccagccag aactcgtggg
     1321 tagtgtacaa cttcgcctct gacggcagca acatgctcgg aggcaacatc tgcggagtct
     1381 tgtatggagg tggcgcggcc tgggggaagg cggcagatgg gacagcacga ttccagacgg
     1441 tggtttcgaa caagctgacc gagtggagaa tcctgccgga ggcggttatg ctgaacccgc
     1501 cggatacgac taagtggacc aacttcgata gcaccgggaa gatagaggtg ctgcttccta
     1561 tgaccggctg cacccagacc gccagggaca ctgtaaacgg cttcctgtac gtaacgcagt
     1621 acaacgctac aagccaggta tccctcagac gcttccaatg gaccggctcc ggctacacca
     1681 tcgatgacac gtggctgccg gaggtgggct cgaacgtctg cggtgtggct gtatgcgcgg
     1741 tcgatccgaa catcctgttc gtgaccaggc agtcgggcgt aacgaacctg gagaggtggt
     1801 atgtggagca cccaagccca tcgatatccg gcccggcgct gctggatgcc tacaaggtgg
     1861 ccgatatagt ggtgcacaac gttgcctccg attccagggg gaacgctctc tttgatgcgg
     1921 gaggctcgga tagaaacagc gtgcagcgga gcatcgggat gttcattttc cccgacggcg
     1981 gttccgagga caccagactg gccggcccct tctaccacga cgggcctgct aataccctcg
     2041 cattggagca tgtgcgcgac gtggcaacgg ttcccgccgg ctcaagcgtt tctcttgagg
     2101 gcaagatcgt gagcgcgaag ttcggaagcg ttctctacgt gcaggaggag gaccgctcgg
     2161 ccggcgtccg ggtggagccg gcgtcacagg tcgaggctca accggggacc agcgtgaagg
     2221 tggtcggcac cgtagactgc tcccttggtg agaggcggat cacagacgca caggtcacgc
     2281 cgggaatcac aggggaggct ccgaagcctc tcatgatggg cacgaggttc gcagacaacg
     2341 acccttcgga gcaactggag ttcgtcggac tgaacaacct cggccttctg gtgcgggtgg
     2401 caggccgcgt gaccgccgtg gacagcgcga acagcttgtt ctacgtcgat gacggccaag
     2461 ggctggacaa cggctttggc agcagaggga tcgccgtgag cttctccggc atgggcccgg
     2521 atgtcaggca ggaggatgtg gtctccgcca caggaatctc aagctgccgc aagctggtgc
     2581 tttcggagag caggactatc aacggacacg aggcgaactc ggggacaacg gtctacgaac
     2641 cgaccatact cctgcggggt gtatccgatg tgttcgtgat caagaggccc tgagcatagc
     2701 tcg
//
LOCUS       DATALS010000521         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_313910, whole genome shotgun sequence.
ACCESSION   DATALS010000521 DATALS010000000
VERSION     DATALS010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313910"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VHS06_11630"
     CDS             <1..580
                     /locus_tag="VHS06_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2988808.1"
                     /translation="ERARSLGISLLDIHMDRLADIDPVITKLGDAIGQKPKADQLLTQ
                     LHQQLAAVAKQVEGQPKVRTLIALDHTARYVVGPRNYLDDILTLAGGTNVAGANAKEY
                     PEIDRETLLQLDPEAIILLLPEAPPQVVESAKSFFATLPQLKAVRTGRVYIHTQWYLL
                     QPTARVRIVAQIIATDLHPLTATTPTTTTVAQ"
     gene            672..1619
                     /locus_tag="VHS06_11635"
     CDS             672..1619
                     /locus_tag="VHS06_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX2988809.1"
                     /translation="MLLLVCLAGWAIIAVGCMALGSTGFGWPKTEMIWSLRREATLVA
                     SLIGASLALAGVTYQAVLRNPLAEPYLLGVSSGASLAVFAWSTISFATLYLASISQQA
                     AAFLGGIGTLAIVFLLAQRRGRLEPLTLLLVGVIINAINGALFLLVFFLARSTDLQKA
                     FRFLAGGLQPDLAQSQVLTVAILFAVSLAILLIISGQLNISMLSEAEAQSLGVRIHRL
                     RWMAMVVASLATASAMAISGPIGFVGLVCPHLGRLLVGNDQRKLIPVATALGASLLAI
                     ADACSRTMGQLPVGVLTGFLGGPFFLFLLWQSRKQGDRT"
     gene            1616..2407
                     /locus_tag="VHS06_11640"
     CDS             1616..2407
                     /locus_tag="VHS06_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2988810.1"
                     /translation="MTTPHRSPLLIATALDFAYSTRPVLHDVAIELFAGQVVALIGPN
                     GSGKSTLIKLLCGLLNTEAQITVRDLPLHQWHRRLLARTVAYLPQSPTYEPADRVADV
                     LRLGRACYWTAFGLETEHDLAAVARITDLLELNDLVDRRMDQLSGGQRQRVFIGRCLV
                     QEPAILFLDEPNTYLDLKHQVDLLKLLQTLAHDHGLCVLMASHDINLAGAFADQLILL
                     SDGRITARGRAAEVLQPDLISRAYGLDMTQIPFPEHPLLFPKLRP"
     gene            2559..>2739
                     /locus_tag="VHS06_11645"
     CDS             2559..>2739
                     /locus_tag="VHS06_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988811.1"
                     /translation="MAIWLLTLELTTASLLMFAVTSITRQKRRPADRLLAASIISLIS
                     ILVPLAIPLAITLMLW"
BASE COUNT          455 a         1094 c          682 g          508 t
ORIGIN      
        1 ggagcgtgcc agatcactgg gaatcagcct gctcgacatc cacatggatc gcctcgccga
       61 catcgatccc gtcatcacca agctcggcga tgcgatcggg caaaagccca aagccgacca
      121 actcctcacc caactgcacc aacagctcgc ggccgtcgcc aaacaggtag aaggccaacc
      181 caaggtgcgc acactcatcg cactcgacca cacggcacgc tatgtcgttg gcccacggaa
      241 ctacctcgat gacatcctga ctctagccgg cggaacgaat gtcgccggtg ccaacgccaa
      301 ggaatacccg gagatcgacc gcgaaaccct cctgcaactc gaccccgagg ccatcatcct
      361 tctgctgccc gaggctccgc cccaggtcgt cgagtccgca aagtcgtttt tcgccactct
      421 gccccaactc aaagccgtgc gtactggccg cgtctatatc cacacccagt ggtatctcct
      481 gcagcccacg gcccgcgtcc gcatcgtcgc gcagatcatc gccaccgacc tgcatccctt
      541 gaccgcaacc acgccaacca cgacaactgt ggcacaatga ggctctccct ctgagatccg
      601 gccacatgtc gtctcccgcc cacacaacct ctccccggaa tctgcctcgc tggagcgccg
      661 agcatctggc agtgctgctc ttggtctgcc tcgccggctg ggccatcatc gcggtcggct
      721 gcatggcgct gggctccact ggattcggtt ggcccaagac agagatgatc tggtcactcc
      781 gacgtgaggc aactctggta gcttccctca tcggggcctc actggccctc gcgggcgtga
      841 cctaccaggc cgtgctccgc aatcccttgg ccgagcctta tctcctgggc gtcagcagcg
      901 gcgcttccct cgcagtattc gcctggtcaa ccatcagctt cgccacgctc tacctcgcca
      961 gcatctcgca acaggcggcc gcctttctcg gcggcatcgg caccctcgcg atcgtgtttc
     1021 tgctcgccca gcgtcgcggc cgacttgagc cgcttaccct cctcctcgtc ggcgtcatca
     1081 tcaacgccat caacggcgcc ctgttcctcc tggtcttctt cctcgccaga agcaccgacc
     1141 tccagaaagc attccgcttc ctcgccgggg gactgcagcc cgacctggcc cagtcgcagg
     1201 tgcttaccgt ggcgatcctc ttcgcggtct cgctcgccat cctcctgatc atctccggcc
     1261 aactcaacat ctccatgctc agcgaagccg aagcccagtc gctcggggta cgcatccatc
     1321 gcctgcgctg gatggcaatg gtcgtggcgt ccctcgcaac cgcgtcggcc atggccatca
     1381 gcggcccgat tgggtttgtc ggactggtct gcccgcacct cggccggctg ctcgtgggca
     1441 acgatcagcg caagctgatc cccgtcgcca ctgcactggg cgcctcattg ctggcaatcg
     1501 ccgatgcctg ctcacgaacg atgggtcagc tccctgtggg cgtgctgact ggcttcctcg
     1561 gcggcccgtt cttcctcttc ctgctctggc agagccgcaa gcaaggagac cggacatgac
     1621 caccccccac cgctctcccc tgctcatcgc cacggctctg gatttcgcct actccacccg
     1681 cccggtgctg cacgacgtcg ccatcgaact ctttgccggc caggtcgtcg ctctgatcgg
     1741 ccccaatggc tcgggcaagt ccacgctcat caagctgctc tgcggcctgc tgaacacaga
     1801 agcccagatc accgtccgcg acctccccct gcaccagtgg cacagacgcc tgctcgcccg
     1861 caccgtggcc tacttgccgc agagccctac ctacgaaccc gccgatcgcg tcgccgatgt
     1921 cctacgactc ggccgggcct gctactggac ggccttcggc ctggagaccg agcacgacct
     1981 cgccgcggtc gcccgcatca ccgacctgct cgaactcaac gatctcgttg atcgccgcat
     2041 ggaccagctc tccggcggcc agcgccaacg cgtcttcatc ggccgctgcc tcgtccagga
     2101 gcccgccatc ctcttcctcg atgaacccaa cacctatctc gaccttaagc accaggtgga
     2161 tctgctcaag ctcctgcaga ccctcgcgca cgaccacggc ctctgcgtcc tgatggcctc
     2221 ccacgacatc aacctcgccg gcgcctttgc cgatcaactc atactgctga gcgacggccg
     2281 catcaccgcc cgcggccgcg ccgccgaagt cctccagccc gacctgatca gccgcgccta
     2341 cggcctggac atgacccaga tccccttccc cgagcacccc ctgctcttcc ccaagctccg
     2401 cccgtagtcg ctcgtgctcc tcgctcgtag tcttcgctcg tagtcgaggc ttcggccgag
     2461 acgaatgctt aggagcccct tcccacctga atccccaacc gcacccgcac ccgcccccgt
     2521 cccttgcgtc ccccgctcca ccacccgagg atatccccat ggccatctgg ctcctcaccc
     2581 tggaactgac aacggcatcg cttctgatgt ttgcagtcac ctcgatcacc cgtcagaagc
     2641 gtcgcccggc cgatcgcctc ctggccgcca gcatcatcag cctgatcagc atcctcgtgc
     2701 cgcttgccat tccgctcgcc atcaccctga tgctctggt
//
LOCUS       DATALS010000522         3787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_448289, whole genome shotgun sequence.
ACCESSION   DATALS010000522 DATALS010000000
VERSION     DATALS010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448289"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VHS06_11650"
     CDS             complement(<1..198)
                     /locus_tag="VHS06_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988812.1"
                     /translation="MERGDHPDSSQPYAKVGGKEVPETKALFQLPSMQNRLGAKLGDK
                     VVLAGFDLQKTKARPGDTVRLT"
     gene            181..1032
                     /locus_tag="VHS06_11655"
     CDS             181..1032
                     /locus_tag="VHS06_11655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2988813.1"
                     /translation="MVTTFHDLRVPYLFPKAGPLRPRALRTMDRFSHASVLTNEPDLA
                     DLGGSETQDTKGNYKKRWLIPIGSNVDNAPPPDFDRQVWRRQLDVDDNTLLVSYFGFM
                     NDTKGVEFLVQALGLLLGRGVPLKLLVVGGETGDTDPTNRSYSQRVHRLIQSRHMEDR
                     VYWTGFVSAQQVSAALLSSDICALPFRDGASVRRGSLLAAILHGLPVVTTFPEKPEPL
                     LVDGENLAMVERDSPKALADTIESLWSDPGARQRLSDGAAMLAGNFTWQKIAARHVEM
                     YETLLNS"
     gene            1262..>3787
                     /locus_tag="VHS06_11660"
     CDS             1262..>3787
                     /locus_tag="VHS06_11660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2988814.1"
                     /translation="MGAFIAPLLVAIVTAIALAVRLNNLGVQSLWVDEAMSVVFASKP
                     LPELMQLLVTEDIHPPLYPVFLHYWMTLAGNGEYAARFPSAIAGALLIPLIYLAGSRL
                     ERLTDPEDRPAISLVGLVASVLAAGSAYYIGFSQEARNYMAVTLAGLLSSYLLLLALA
                     GRSRRLWIAYGLATLAALYTHYTAALLLLSQAAFVLLVRKPYGDAWKRWLLTTLAVGI
                     CFLPWLGFSIAQIQRISDYWPGTLRLDAAIRTSLLQFVVGGGADLQGMVLPMALGVGL
                     LLLGILALLVGGSRRNFSQPAFFLLLYMLIPAALIFGVAYFRPKFDPRYLLIITPAFY
                     LTLAWGIAAMLRQAVQPDRIILLRVLFPILGIAALAGSVAISIVYGEPTKVMHVGDGS
                     GASAEYGDYRALVSYLESQAEPGDAVVLMMNTYHPYVYYSTGQIPWYPLEPYDDTDGA
                     IILLNRIAQQHKRLWFILWQKDWVDPADYVMHTMETQAQEMPLDKSFGGLGLRLFQVI
                     PGQPFSYYPKVDHKQEALFGPSRLEFWGWNSSTDSAASGDSIQYDLHWRPNNKIDANY
                     KIVLSLVDADRHVWARTDEVMATALYPSSRWRVQEIVHDRHKLAIPPGTPPGNYQVEL
                     HLYDPATMKEVSAARWSGAGLGTSISLGTVNVRPTPESSLSPPGQSPMATWDLSSGDR
                     MELLGAKVDRSSAKAGEPVEITLQWRSPKQPLAAYIVRLALLDDKGRPTGEQLLPLVQ
                     GYPTYRWRAGEAVVSKHWYTLPVDQQSGKYGLVVAVLPLDEKDKAPIRYAQVASLEVP
                     ETKALFQLPSMQNRLGAKLGDKVVLAGFDLQKTKARPGDTVRLT"
BASE COUNT          710 a         1222 c         1125 g          730 t
ORIGIN      
        1 tgtcaggcgc accgtatccc cagggcgggc tttcgtcttc tgcaggtcga agcctgcaag
       61 gacgaccttg tcgcccaact ttgcacccaa ccgattctgc atcgacggta gctggaacag
      121 cgccttggtt tccggaacct ccttcccgcc taccttcgcg tacggctgcg aggaatcagg
      181 gtggtcacca cgttccacga cctgagagtc ccatacctat tcccaaaggc cggccccctt
      241 cgccccaggg cgctgcgtac gatggaccgg ttcagccacg cctccgtact aaccaacgag
      301 cccgatctcg cggatctcgg aggttcagag acccaggaca caaagggcaa ctacaagaag
      361 cgatggctga tcccgatcgg gagcaacgtc gataatgccc ctccgccaga cttcgatcgg
      421 caagtgtggc ggaggcagct ggatgtggac gacaacacac tgctcgtgtc ctacttcgga
      481 ttcatgaacg acacgaaagg tgtggagttc ctggttcaag cgctggggct gctgctagga
      541 agaggcgtcc cgctgaagtt gctggtggtg ggaggcgaga cgggagacac cgaccccacc
      601 aaccgctcct attctcagcg ggttcaccgt ctcatccaat cacggcatat ggaagatagg
      661 gtatactgga ccggctttgt gtccgcacag caggtgtcgg cggcgctgct ttcatcggac
      721 atctgcgctc ttcccttccg cgacggtgca tcggtgcggc gtggcagtct tctggctgcc
      781 atcctccacg ggctacctgt ggtgactacc ttcccggaga aaccggagcc gttgctggta
      841 gacggcgaaa acctggcgat ggtggagcgg gatagcccca aggcccttgc agacacaatc
      901 gagtcgttgt ggagcgatcc gggagcgagg caaaggctct ccgacggtgc cgccatgctg
      961 gccggaaact tcacatggca gaagatcgcg gccagacatg tggagatgta cgagacattg
     1021 ctgaatagtt agcccgcggt ctgttcgatc actactgcga cctgtttctc taacactgtg
     1081 atctcgacac taggagttga ggtcgtccct gacctcgacg gccccttccg cgaccgcccg
     1141 ggtcagggac gacccggtct cctagatcag gatcaccgtg tcgcagaatt acggtctcgc
     1201 taccgaggtt gcgcgtttgg acaacactac tgatattgga tctcgaggag cgaggcgatt
     1261 gttgggcgcc ttcatcgccc cgctcctcgt tgcgatcgtc acggccatcg ccctggcggt
     1321 acggctgaac aacctgggcg tccagagcct ctgggtggac gaagcgatga gcgtcgtctt
     1381 cgcatccaag ccgctgccgg aactgatgca actgctggtc accgaggaca tccacccacc
     1441 gctctatccc gtgttcctcc attactggat gaccctcgcg ggaaacggtg agtacgcggc
     1501 cagattcccg tccgccatcg cgggcgcgct gctcataccg ttgatctacc tcgccggcag
     1561 ccggctcgag cggctgaccg atccagagga tcgtccggcc atcagcctgg ttgggttggt
     1621 ggcttcggtg cttgccgccg gttcggccta ctacatcggg ttctctcagg aggcccgaaa
     1681 ctacatggcc gtcaccctgg ccgggcttct ctcctcctac ctgctgctgt tggcgctggc
     1741 aggccgcagt cgccggctct ggatagcata cgggctggcc acgctggcag cgctgtacac
     1801 tcactacacc gccgccctgc tcctgctatc ccaggcagcg ttcgtgctgc tggtccggaa
     1861 gccctacggc gacgcgtgga agcgctggtt actcaccacc ctggccgtgg gcatctgctt
     1921 cctcccctgg ctggggttct ccatcgctca gatccagcgc ataagcgact actggcccgg
     1981 aacgctgcgg ctagatgcag ccatccgaac ttccctcctc cagttcgtag tgggcggcgg
     2041 cgccgatctt caaggcatgg tgttgccgat ggcgctgggt gtgggcctcc tcctattggg
     2101 catactggca ttgctggtgg gaggctcccg ccggaacttc tctcagcccg ccttctttct
     2161 cctgctgtac atgctcattc ccgcggcgct catcttcggc gtagcctact tccggccgaa
     2221 gttcgacccg cgctaccttc tgatcatcac gccggccttc tacctgaccc tggcgtgggg
     2281 catcgcggcc atgctgcgac aggccgtgca accggatcga atcatcctcc tcagggtcct
     2341 gtttcccatc ctgggcatcg cggcactcgc cggatcggtg gcaatttcca tcgtgtacgg
     2401 tgagcccacc aaggtcatgc acgtcggcga cggcagcggc gcatccgccg agtacggcga
     2461 ctaccgagcc ctcgtctcat acctggaatc gcaggcagag cccggggacg ccgtcgtgct
     2521 gatgatgaat acctatcacc cttatgtcta ctacagcacc gggcagatcc cctggtaccc
     2581 gttggaaccg tacgacgaca cggacggtgc gataatcctg ctgaacagga tagctcagca
     2641 gcacaagagg ctctggttca tcctttggca gaaggattgg gtagacccag cagattacgt
     2701 tatgcacacg atggagaccc aggcccagga gatgccgctg gacaagtcct tcggcgggct
     2761 cgggctccgg ctgttccagg tcatccccgg ccaacccttc agctactacc cgaaggtaga
     2821 ccacaagcag gaagccctgt ttggcccttc tcgactcgag ttctggggtt ggaactcatc
     2881 caccgacagc gcagccagtg gcgactccat ccagtacgac ctgcactggc gtcccaataa
     2941 caagatcgac gccaactaca agatagtgct ctcactggtg gatgccgatc gccacgtctg
     3001 ggcacgcacc gacgaagtaa tggctaccgc gctctatcca tccagccgat ggagggtgca
     3061 ggagatcgtt cacgaccgcc acaaactggc catacccccc ggcacgccgc cgggcaacta
     3121 ccaggtggag ctacacctat acgacccggc gacgatgaag gaagtgtccg ccgcgcgctg
     3181 gtccggagca ggactgggta cgagcatctc gctcggcacc gtcaacgtgc gacccacgcc
     3241 ggagagcagc ctctcaccgc cagggcagtc gccgatggcc acctgggacc tgtcgtccgg
     3301 ggaccggatg gagttgctcg gggccaaggt cgaccgctcc tcggcaaagg ccggagagcc
     3361 ggtagagata acgctccagt ggcgctcccc taaacagccc cttgccgcct acatagtcag
     3421 gctggcgctg ctcgatgaca agggaagacc cacgggcgag caactgctcc cgctggtaca
     3481 gggttatccc acctataggt ggcgtgccgg agaggccgta gtctccaagc attggtacac
     3541 gttgcctgtc gatcaacaat ccggaaaata cgggttggtg gtcgcggtcc tcccgctgga
     3601 cgagaaggac aaggcaccga tccgctacgc tcaggtggcg agcctcgagg ttccggaaac
     3661 caaggcgctg ttccagctac cgtcgatgca gaatcggttg ggtgcaaagt tgggcgacaa
     3721 ggtcgtcctt gcaggcttcg acctgcagaa gacgaaagcc cgccctgggg atacggtgcg
     3781 cctgaca
//
LOCUS       DATALS010000523         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_77596,
            whole genome shotgun sequence.
ACCESSION   DATALS010000523 DATALS010000000
VERSION     DATALS010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77596"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1831)
                     /locus_tag="VHS06_11665"
     CDS             complement(188..1831)
                     /locus_tag="VHS06_11665"
                     /inference="COORDINATES: protein motif:HMM:NF004807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX2988815.1"
                     /translation="MKGSSAVAEILKREGVDTFFCFPINPIIDAAAELGIRPIIARTE
                     RTVIGMADGYSRVTNGRRIGVAGTQFGPGVENSFGGVAQAFSDSTPLLFLPAGPKQNR
                     LGFEPTFDAVKNFQYVTKWAARVNMVDRIPELLRRAFTLARTGRPGPVLLEMPMDVMA
                     SDLEDALLEYSAVRAPRSMASHADVAAAVKAIVTARKPLIHAGVGILYAEAWDELREF
                     AELIQAPVMTTLQGKSAFPEDHSLSLGSGGAAGPRHLAQWLDEADLIFGAGSGLAISS
                     YDAPIPRGKVAVQLTVDERDLNRDYTTEFLLMGDAKLVLQQMIEEAQRQLGKDGRKGD
                     DRVTREVKAARDEWMGEWMPKLTSNEVPINPYRLVWDLQHAVDLRNTIATHDAGSPRD
                     QMTPFWPALEPNSYIGWGKSTHLGYGIPLVLGAKVAKPEKTVLNVMGDLAFGMSGLEL
                     ETAARNRLGTLTIVFNNGVMGDYTKHIGRSDQLYGTRRITGSYAKIAEGLGAYAERVE
                     KPDEIIPAVKRALQVNQDGRPALLEVMTREETQFSKYWK"
     gene            2190..2274
                     /locus_tag="VHS06_11670"
     tRNA            2190..2274
                     /locus_tag="VHS06_11670"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2224..2226,aa:Tyr,seq:gta)
     gene            2295..2367
                     /locus_tag="VHS06_11675"
     tRNA            2295..2367
                     /locus_tag="VHS06_11675"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2328..2330,aa:Thr,seq:ggt)
BASE COUNT          404 a          728 c          752 g          483 t
ORIGIN      
        1 gtcgcggcct caccgcctat ggtcccaaca acgatggagg tggctcctgc tatgccggag
       61 aagacggtgc acgctgccgg ttcgagcaga tcctgcggaa cctgcccacc ctgccgtagc
      121 cgccgtctcc tccatttctt ccacgccgag ccggagggga agacgttgct cgccctctcc
      181 tcccacgcta cttccagtac tttgagaact gggtctcctc cctggtcatc acctccagga
      241 gtgcaggacg gccatcctgg ttaacctgca gcgcgcgctt gaccgcgggg atgatctcgt
      301 ccggtttctc taccctctcc gcgtatgccc ccagcccttc agcgatcttg gcgtagcttc
      361 cagtgatgcg acgagtgccg tacagttggt ctgagcgacc gatgtgtttg gtgtagtctc
      421 ccatgacacc gttgttgaac acgatggtca atgtgcccag ccggttgcga gctgcggtct
      481 cgagttccag gccgctcata ccgaaggcca ggtctcccat cacgttcagg acggtcttct
      541 ccggcttggc aaccttggct ccaagtacca gcgggatgcc gtagcccagg tgagtggact
      601 tgccccagcc gatgtacgag ttgggctcca gggcgggcca gaagggggtc atctggtccc
      661 tggggcttcc agcatcgtgg gttgcgatag tgttgcggag gtcgaccgcg tgctggagat
      721 cccagaccaa cctgtaggga ttgatgggga cctcgttgga ggtaagcttg ggcatccact
      781 cgcccatcca ctcgtccctg gccgccttga cctctctggt gacccgatcg tcgcccttgc
      841 ggccgtcctt ccccaactgc ctctgagcct cctcgatcat ttgctgcagc accagcttgg
      901 catcgcccat caggaggaac tccgtggtgt agtcgcggtt caggtcgcgc tcgtccacgg
      961 tcaactgcac tgcaaccttg cccctgggga taggcgcgtc gtagctggag atggccagcc
     1021 cgctgccggc tccaaagatg aggtctgcct cgtcaagcca ttgggcgagg tgtcgagggc
     1081 ccgccgcgcc gccgctgcca agagagagcg aatggtcctc cgggaatgcg ctctttccct
     1141 ggagggttgt cataacgggt gcctggatca actcggcgaa ctcccgcagt tcgtcccagg
     1201 cctcagcgta cagaatgccc acacccgcat ggataagcgg cttgcgtgcg gtgactatgg
     1261 ccttgaccgc ggcagcgacg tccgcatggc tggccatgga cctgggggcg cgaacggcac
     1321 tgtactccag cagggcatcc tcgagatcag aggccatgac gtccatgggc atttccagca
     1381 ggaccggccc aggccgtcct gtcctcgcca aggtgaaggc ccggcggagg agttctggga
     1441 ttcggtcgac catgttgact cttgccgccc acttggtgac gtactgaaag ttcttgacgg
     1501 cgtcgaaggt gggctcgaag ccgagtcggt tctgtttagg tcccgccggc aggaagagca
     1561 gcggggtgga atctgagaac gcctgggcga cgccgccgaa ggagttttct acgccgggcc
     1621 cgaactgggt tccggcgacc ccgatcctcc gcccgttggt caccctggag tagccgtcgg
     1681 ccataccgat gactgttcgc tcggtgcggg cgattatggg tctgatcccc aactcggcgg
     1741 cggcgtcgat gatggggttg atggggaagc agaagaaggt atcgactcct tcccgcttca
     1801 ggatttcggc aacagccgag gagcctttca tgtaatcctc ccatggtact gctggcaagg
     1861 ttgtggaaac gagtgccact gctatactga gcgagggcag cctggagcgt gtcctgtttg
     1921 ggcctggccg cgcaaggtta tccttgcgga gaccatgctt cgacaccgca cgcggggagc
     1981 tctgcgccgg agcttctagt gcaacgatca tgccaccgct gagcccggct ggcctacggc
     2041 cgcctcatgc cggcgccggg agtggatcgg ggcgagtctg aaggggcata gtatgctgcc
     2101 gttgacgaag ccgaattggt tcggtataat tccccggtgc ttaggcaccc ccgcacacgt
     2161 gagcggtgac gcttgcgtga gtgaggcggg gaggggtggg tgagtggcta ataccaacgg
     2221 tctgtaaaac cgtcgccctg acgggctaca caggttcgaa tcctgtcccc tccacccgga
     2281 aaacccatag gcgagcccac atagctcagc aggtagagca cggtcttggt aagaccgggg
     2341 tcatcagttc gaatctgatt gtgggct
//
LOCUS       DATALS010000524         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_314873, whole genome shotgun sequence.
ACCESSION   DATALS010000524 DATALS010000000
VERSION     DATALS010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314873"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VHS06_11680"
     CDS             <1..229
                     /locus_tag="VHS06_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX2988816.1"
                     /translation="YDPLYVANEGKLVAILPEQEAEAALSAMRRTRYGEEARIIGRVL
                     AAPAGKVLMKTRIGGTRIVQMLMGEMLPRIC"
     gene            complement(284..1939)
                     /locus_tag="VHS06_11685"
     CDS             complement(284..1939)
                     /locus_tag="VHS06_11685"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX2988817.1"
                     /translation="MQQSAQRLSRLPSSLPLSSRVVSLLLFAGLSGVAFLSGIAVMGP
                     TRLVGMQATWLMTFVILLVGFSLWWAIYLRLLTLLGGLNGSEAIGRSLLPILLLDLSI
                     VPNLAVLLVGKLPYSMTFDANVQSLFQWMAGSTVFVVFVMQELVLLGAARNEDLRRLA
                     GDAIRTVSVVLSRVGERRNCFAGRLRSYWLLLIVMLAGFAVRLTVIWDAHPGDMSDTL
                     SVAYSILSWPPFGYYHSYRPQTSVYAHLPLYPVLIAPFYWVFENIAKLPTMWVAKLIS
                     AVADLVVAVMIYRQAKGHWRQLWGTVLSAAYLLSPLVISSDDHAVPTAAAFTIAAFAS
                     LERPWLCGLMIALASATRNEAAFFGLPLVIHFLFRRQVQEWVVFLGVYLTTLVAFALP
                     FVLTDPEAIDYAMRRQSLRQAAPEVSSLLTVLQPHLSQEFYTLLQKNPLLLTLVGTLS
                     GALLAVRDRRVMRVALVVGTVYVMTLPVLHPRYLVFIYGGGLFYAARYGNPLIALAFG
                     IATWPGIPFGPTLQLVMVAILTICGLLQVYWPRRATRLAPLTQ"
BASE COUNT          534 a          640 c          688 g          351 t
ORIGIN      
        1 ctacgacccc ctctacgtgg ccaacgaggg aaagctggtg gccatcctgc cggagcagga
       61 ggcggaggct gcgctgtccg ccatgcgtcg aacccgttat ggggaggaag cacggatcat
      121 cgggcgcgtg ctcgcggccc ctgcgggcaa ggtgctgatg aagacccgca tcggcggcac
      181 gcgaatcgtt cagatgctga tgggggagat gcttccccgc atctgctagt cctaaaccgt
      241 ggcctagaca cgtagggagg cgcttggcgc ctccctgttg ggttcattgt gttagaggag
      301 ctaggcgggt ggcacgcctc ggccagtaga cctggagcag gccacagatc gtgaggatcg
      361 caaccatcac caactgcagc gtgggcccga aggggattcc gggccaggta gcgatgccga
      421 aggccagagc tatcagagga ttcccgtatc gcgccgcgta gaacaatcct ccgccataga
      481 tgaacaccag gtaccgcgga tgcagaaccg gcagagtcat gacgtagact gtcccaacga
      541 ccagcgcgac cctcatcact cgacggtccc gcacggcgag aagcgcaccc gataacgtcc
      601 ccaccagggt cagcagcagc ggattcttct gcagcagagt gtagaactcc tggctcaaat
      661 gcggctgcaa gacagtgagt aggctggaca cctccggcgc agcctgtcgt aggctctgcc
      721 tgcgcatggc gtagtcaatt gcctcgggat ctgtcagcac gaatggcagc gcaaacgcaa
      781 ccagcgtcgt cagatacact ccgagaaaca ccacccattc ctgcacctgt cgtctgaaga
      841 ggaaatggat gaccagcgga agcccgaaga acgcagcctc gtttcgagtg gctgaagcca
      901 gtgcgatcat aaggccgcac aaccaggggc gctccaggct cgcgaaagcg gcgatggtga
      961 acgcggcagc agtcggcacc gcgtggtcat ccgagctaat caccagaggc gagagtagat
     1021 aggctgcaga gaggaccgtt ccccacagct gcctccaatg tcccttcgcc tgacggtaaa
     1081 tcatcactgc caccaccaga tcggccacgg cagagatgag cttcgcaacc cacatggtcg
     1141 gcagctttgc gatgttctcg aagacccagt agaacggggc tatcagcacg ggatatagag
     1201 gaaggtgcgc atacaccgag gtctgcggac ggtacgagtg gtagtagccg aaaggcggcc
     1261 aactcaggat cgagtacgcc acagaaaggg tgtcgctcat gtcgccgggg tgagcgtccc
     1321 agatcacggt gaggcgcacc gcgaatccgg caagcattac gatcaagagc agccaatagg
     1381 agcggagcct gccggcgaaa caattccgcc gctcgcctac tcgtgataga acgactgaca
     1441 ctgttcgaat ggcatcgccg gccagtctgc ggaggtcttc atttcgtgca gcaccgagca
     1501 ataccaactc ctgcatcaca aacaccacaa agaccgtcga tcccgccatc cattggaaga
     1561 gcgactgcac gttcgcatcg aaagtcatcg agtagggaag cttccccaca agcaacacgg
     1621 ccaggttcgg cacgatggag aggtccagaa gaaggatcgg gagcagggac cggccgatgg
     1681 cctcactgcc gttcagaccg ccgaggagag tcagtaatcg caggtagatg gcccaccaga
     1741 gggagaatcc tacgagaagt atcacgaagg tcatcagcca ggttgcttgc attccgacga
     1801 gcctggtcgg gcccatgact gctatgccag aaagaaaggc gactccgctc agccccgcga
     1861 aaagcaacag actgaccacc ctggatgaca ggggaagcga ggaaggcaaa cggctcagcc
     1921 tttgggcgct ctgctgcaag aaaatgaatc ccccttgagg atggtagtcg cggatcgttg
     1981 tgagtgcgtg ccgaggcaat caccgcggca aagagggtag cgctcgagtc cgagacaagg
     2041 agacgacgac ccgtgagcac tgtatcagag ggggatggcg gcttgcaaca gcggtgcact
     2101 cgttcgccgc ctgcgtcgat gatgcagata tcagtgatat gattatgtgg acgcgcgatg
     2161 cacgctaggt gcaagaaagc agcgaagcgc tgaggagata acacccacat gga
//
LOCUS       DATALS010000525         2698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_314941, whole genome shotgun sequence.
ACCESSION   DATALS010000525 DATALS010000000
VERSION     DATALS010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314941"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1756
                     /locus_tag="VHS06_11690"
     CDS             149..1756
                     /locus_tag="VHS06_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEX2988818.1"
                     /translation="MSGHVFITCGDLTKLSCDAWLLPADAGCFVTHHWWEGVSDDLRR
                     QLAGLRYGDTPRPDNWGDRGARVIPLLGLQTELDQPCPYLVNVGGGLGSPIDWFMEGA
                     RQFFQVVAEQHRGEGHHRRPRPLVALPLIGTGHGGAGHVKGEVVRALVDELYKAAEKY
                     GFDVALVTDSVQPFVAAQNARWQHLGSLQGTATCDLWPELSDKLAASARTLARRASTG
                     GLVLFIGSGVSADAGLPSWESLLNGLARDAGMDGREQDALAKLSILDRPRIIESRLAA
                     KGRSIGKAVCDRLMEKCYSLMHSLLATLPISEVVTTNYDCLFEDASWAAGRATAVLPY
                     QPVSGSSRWLLKMHGCVNHSEDIVLTREDYLRYPDRRAALAGIVQALLITRHMLFIGF
                     SLIDDNFNRIVDEVRKAIGGTDHQTLDGKPFGTAILLQKDELLEELWRKDLNIVSMFD
                     SAEVSIPEGARRLEIFLDYLLAEASRATSPLLDEAFEGILTDDERAVRDLLKSLMQGA
                     SKDLQRSPAWQPVAELIERLGGPSVQR"
     gene            1820..2119
                     /locus_tag="VHS06_11695"
     CDS             1820..2119
                     /locus_tag="VHS06_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX2988819.1"
                     /translation="MTARIELDREKIGEFCRRHHIRRLSLFGSVLRQDFRPDSDVDVL
                     IEFEPGKAAGLIRLAGMEQELSELVGRKVDLRTPAELSRYFREEVLATAEVQYAA"
     gene            2382..>2698
                     /locus_tag="VHS06_11700"
     CDS             2382..>2698
                     /locus_tag="VHS06_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988820.1"
                     /translation="MPGRGSTTDESRRLREAIGSVALEMASELDWDRAVEAALRVAES
                     SLNADAIILWTTNDRERVLELTAQRGRRAEVHLPPHQSRQLLLHSGKPDEPGRLSRLE
                     LNE"
BASE COUNT          505 a          780 c          878 g          535 t
ORIGIN      
        1 ggagcgggag ccttcctgcg caaaccatct ggactgccga agcttgcggg cgcggtccgc
       61 gccgagctgg atcgggatgg cgtggcgggc tgacacgaga tcgtggtctg ctcagtatcg
      121 cgcgagagcc acacgaggag gacagactat gtccggccat gtcttcatca cctgcgggga
      181 tctgacgaag ctgagctgcg acgcctggct attgccggcc gatgccggct gcttcgtgac
      241 ccatcactgg tgggaaggtg tatcggatga tttgcgaagg cagttggccg gactgcgata
      301 tggcgataca ccaagacccg acaactgggg ggatagaggt gccagggtca tcccgttgtt
      361 gggtctgcag acggagcttg atcagccatg tccatacctg gtcaatgtcg gtgggggact
      421 ggggagcccc atcgattggt tcatggaagg ggctcggcag ttcttccagg ttgtggccga
      481 gcagcatcgg ggagaggggc accaccgtag accaaggcca cttgtcgccc tgccgctgat
      541 tgggaccggc catgggggcg ccggccacgt caagggcgag gtcgtgcgtg ccctcgtgga
      601 tgagttgtac aaggcggcgg agaagtatgg cttcgatgtg gctctggtca cagactcggt
      661 gcaacccttc gttgccgcac agaacgcacg gtggcagcat ctgggttcct tgcagggaac
      721 agccacttgt gacctgtggc cggaactctc ggataaactg gccgccagcg ccagaacact
      781 tgcgcgccgc gcttcaacgg gtgggcttgt cctcttcatt ggctccgggg ttagcgccga
      841 tgccggcctt ccctcctggg agtccctgct gaatggactt gcaagagatg ctgggatgga
      901 cggccgagag caagacgctc tcgcaaagct gtcaatcctc gatcgaccca ggatcatcga
      961 gtcccgtctt gcggcaaagg gcaggtcaat tggcaaggcc gtctgcgacc gcttgatgga
     1021 gaaatgctac tcccttatgc acagcctcct ggcgactcta cccatctcag aagtggtaac
     1081 aacgaattac gactgtctat ttgaagatgc gtcctgggct gccggccggg ccactgctgt
     1141 cctcccctac cagcctgtgt caggcagttc tcgttggctg ctaaagatgc acgggtgcgt
     1201 caatcattca gaagacattg tgctcactcg ggaggactat ctgcgctacc cagaccgacg
     1261 agctgctctc gccggcatcg tccaggcgtt gctgatcacg aggcacatgc tctttatcgg
     1321 cttctctctg atcgacgata atttcaaccg aatagtggat gaggttcgaa aagccatcgg
     1381 cgggacagat caccaaacct tggatggaaa gcctttcggg acagccatcc tcctgcagaa
     1441 ggatgagctg ttggaggagc tctggaggaa ggatctgaac attgtaagca tgttcgatag
     1501 cgccgaggtt tccatcccag agggtgcacg gcgcctggag atcttcctcg actacctctt
     1561 ggccgaagca agtcgtgcca cgtcgccgct gctcgacgaa gcctttgaag gcatactgac
     1621 ggatgacgag cgtgcggtgc gcgatttgtt gaagagcctc atgcaaggcg cgtctaagga
     1681 tctccagaga tcgcctgcgt ggcagccggt tgctgagctt atcgagagac tgggcgggcc
     1741 gtcggtgcag aggtgatgcc tggcggtccg cgatccttac gtcttcgagt ctcgtcaatt
     1801 cgattggagg ggaacctgca tgacggccag gattgagctg gacagggaga agataggcga
     1861 attctgccgc cgccatcaca tcaggcggct ttccctcttt ggctccgtgc tgcgacagga
     1921 ctttcggccg gacagcgatg tggacgtgct cattgagttc gagcctggaa aggcggccgg
     1981 gctcatccgg cttgccggaa tggagcagga gctgtcggaa ctggtggggc ggaaggtgga
     2041 cctccgcacg ccggccgagc tgagccggta cttcagggaa gaggttctgg cgacagcgga
     2101 agtgcagtat gcggcgtgac gatcccaccc gcctgcgcca catgctggat gcctccaggt
     2161 tggccatcgc ttcctcaatt ctcctgagca tgtagaaccc cgtccccgag catgctgcta
     2221 tcagccccct aatcgcttca ggtgcgctgc cgacgaggag atcctgttac atctctggtc
     2281 cggtggcacg ctgcctgcca cgttccgggt cgcctagggc cacgcggtag cggctcttcc
     2341 gcgatcgtgc cctgggtgta ccacagcagg ggggcggcga tatgcctggg cgtgggagca
     2401 ccaccgacga atcgaggcgc cttcgagagg ccatcggatc cgtggcgctg gagatggcca
     2461 gcgagctcga ctgggaccgg gcggttgagg cagcgcttcg ggtagccgaa tcctctctca
     2521 acgccgacgc catcatcctc tggaccacga atgaccgaga gcgagtgttg gagttgacgg
     2581 cccagcgagg gcggcgtgcg gaggtccacc ttccgcccca ccagtcccga cagctcctgc
     2641 tccattccgg caagccggat gagcccggcc gcctttccag gctcgaactc aatgagca
//
LOCUS       DATALS010000526         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_125339, whole genome shotgun sequence.
ACCESSION   DATALS010000526 DATALS010000000
VERSION     DATALS010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125339"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VHS06_11705"
     CDS             <1..354
                     /locus_tag="VHS06_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988821.1"
                     /translation="DESTRRERPHDRDLHTAVARMSDWGTGQGLPALAMDEEKWQQLV
                     LKHMPDRDNSAYVNEVIRLVSGEESVRSRLKKVETDSESMEWIQTLLDLATNIWNNTP
                     QPDKGGRSANEMVGL"
     gene            complement(642..869)
                     /locus_tag="VHS06_11710"
     CDS             complement(642..869)
                     /locus_tag="VHS06_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX2988822.1"
                     /translation="MSERIEVNPGVHFGKPCVAGTRIPVQDVLELVAEGVPFVEIIRD
                     YYPDMKVEDIRACVRYALDVLEAEELHVSVS"
     gene            complement(1175..1345)
                     /locus_tag="VHS06_11715"
     CDS             complement(1175..1345)
                     /locus_tag="VHS06_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988823.1"
                     /translation="MPVDKFPAAFFDPNGYLIFPTLNEYDECPHFRIGKRVNVPGGIS
                     SIPGYIRRPHQT"
     gene            complement(1786..>2310)
                     /locus_tag="VHS06_11720"
     CDS             complement(1786..>2310)
                     /locus_tag="VHS06_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988824.1"
                     /translation="SGTGSPAAEATKGSAPGAKPTEAPKAGSSRTAPVPFGQAITLTQ
                     GNRSFEVKVLEVQRDATALVKKANMFNADPKEGMAYVAIKMRLAYLKGPEDKPYSTPS
                     TGESLFAKSRMWGSEVMTVAPEPRLGGNDIFPGATVEGWITPLQAPKDAMDEIVLSWG
                     KDLLGSGEVWFALK"
BASE COUNT          454 a          680 c          667 g          509 t
ORIGIN      
        1 gatgagtcta cacgtcgtga gcggccccac gatagggacc tacacaccgc tgtggcgcgt
       61 atgtcggact ggggaacagg gcagggcctg ccggcgttgg ccatggacga ggagaagtgg
      121 cagcaattgg tgctgaagca catgcccgat cgggacaact ctgcctacgt gaacgaggtc
      181 ataaggctgg tgagcggcga ggagtccgtg cgatctcgct tgaagaaggt ggagactgac
      241 agtgagtcca tggagtggat ccagaccctg ttggaccttg ccacgaacat ctggaacaac
      301 accccccagc ctgacaaagg cggaagaagt gcgaatgaaa tggtggggct gtgacggggc
      361 cgaattgtcg atatgataag caggagaagc ccaaacaact ctcggctaga gccggtgatg
      421 cgaagggaat cgaccctggt gtggccggac caatggatct taggcgtgcc ggtgtgtcct
      481 gtccatgagc cgctccagga atgcggccat ctcctcgtcg gcaagccaac tgatgtcgct
      541 gtgggctttg gctgcctcga attcgtcgac cacgcgctga tcctccgttg cctccggtct
      601 gtccatcccg ccatttaggc tgagaagctc attgctgtcc attaagatac cgatacgtgt
      661 agctcctctg cttccaatac gtctagagcg taccgaacac atgccctgat gtcttcaact
      721 ttcatgtccg ggtagtagtc tcggatgatc tccacgaacg gaacgccttc ggcgaccagt
      781 tccagcacgt cttggactgg gattctggta cctgccacgc agggttttcc gaagtgcaca
      841 ccagggttaa cctcgatcct ttcactcatc cgttacctcg cttggcttgg acttcacctg
      901 catgcaattg gacgctacgt acgtggcctt ggatcaggtg tgcaactgca gaatgtagat
      961 caacagaatc ctctgtctca tcttttctac gggcctggta tctacagatg taaattcaat
     1021 tccagcctgc cagccggaag gttgggttgg cgacagcaca tatctgcagg ataacgagtg
     1081 catgtaatgc tcaagagact tacactgccc tcccgttctg gacgaggcag aaccagcgag
     1141 ttgtgagaca gggcctcgaa gcatttttca catttcaggt ctggtgcggc cgccgtatat
     1201 accctggaat agaggagatc ccgccgggga cgttcaccct cttgccgatg cgaaaatgcg
     1261 ggcactcatc gtattcattc agggtgggga agattaggta gccgttcgga tcgaagaagg
     1321 cagcggggaa cttatccact ggcatgaaga acgtcccatc tttgtggtac cgagattgtg
     1381 tagccccgac attccaccta cggttgagtt cggcgccgga gttcgacctt ggagcgtgga
     1441 tttgccgctt tccatgcatc gttggcttct cctcggacaa aagtctgatc tctacccggt
     1501 cctgttcggc cggtggttgt ctaggatcgc catgcatcgc ccgcaccaat tatgggatgt
     1561 cgcaggataa gtctgcgcgg attgtcacaa aaccataccc gatccaaggc cagacgccca
     1621 gtattgcccc agtctccatg gtactaccag atagtaagcc attccatact aatcgacacg
     1681 gtctggtgcc aaatatatga tgtggctcga agctatctcg cgttgatgga aacgtggggc
     1741 aggcacggga acggatcccc tgcttgttcc cgtgcccgct ttgacctact tcagcgcaaa
     1801 ccacacctcg ccgctgccca gcaggtcctt cccccaagag aggacgatct cgtccatggc
     1861 gtccttggga gcctggagcg gggtgatcca cccctccacc gtggcgccgg ggaagatgtc
     1921 gttcccgccg agcctgggct cgggcgccac ggtcattact tccgatcccc acattcggga
     1981 tttggcgaag aggctctcgc cggtggatgg ggtgctgtac ggcttgtcct ccggaccctt
     2041 caggtaggcc agccgcatct tgatcgccac gtaggccatc ccctccttgg ggtcggcgtt
     2101 gaacatgttg gccttcttca ccagcgccgt cgcgtctctc tgcacctcca gcaccttcac
     2161 ctcgaagctc cggttgccct gggtcagcgt gatcgcctgc ccgaagggga ccggcgccgt
     2221 ccggctgctg cccgctttcg gcgcctccgt gggcttcgca ccgggtgcac tccccttcgt
     2281 ggcctccgcc gccggggagc cggtcccaga
//
LOCUS       DATALS010000527         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_125385, whole genome shotgun sequence.
ACCESSION   DATALS010000527 DATALS010000000
VERSION     DATALS010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125385"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="hypE"
                     /locus_tag="VHS06_11725"
     CDS             complement(<1..977)
                     /gene="hypE"
                     /locus_tag="VHS06_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX2988825.1"
                     /translation="MRGDRLLLAHGSGGKLSAELIQEVFVPAFGNPALARMDDSAILP
                     LPREAANPPLPLGEGRGEGKAPRLAMTTDSHVVKPLFFDGGDIGRLAICGTVNDLSVV
                     GATPLYLSAGFVLEEGLPIADLRRVVESMKLAAEEAGVFVVTGDTKVVEQGSADGLYV
                     TTTGVGIVPEGIDISGSNACEGDVVLLSGTIGDHGIAVLSKREGLEFGSPVASDVAPL
                     NRMVAAMLEVAPHIHAMRDPTRGGLATTLNEFAAQSGVAIQIEEGQIPVRPAVQAACE
                     MLGYDPLYVANEGKLVAILPEQEAEAALSAMRRTRYGEEARIIGRVLAAP"
     gene            complement(974..2062)
                     /gene="hypD"
                     /locus_tag="VHS06_11730"
     CDS             complement(974..2062)
                     /gene="hypD"
                     /locus_tag="VHS06_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX2988826.1"
                     /translation="MKYLDEFRDSEMAARLVERIRRRPPIPVNFMEVCGTHTVSIFRH
                     GIRQLLPENVNLISGPGCPVCVTANGDIDWAIAAAKHPDTILTTFGDMLKVPGSYSSL
                     QQVRAEGGDVRVVYSTLDALQIARSNPSKKVVFFGIGFETTAPTIACSVLEAEEAEIN
                     NYFVFSVHKVMPPPMKALVDSGEVRIDGFLCPGHVSTIIGSEPYRFLAEDYGVPCVIA
                     GFEPLDVLQSIDMLLAQRVEGRAEVEIQYSRVVRPKGNPAALEALYRVFQPTDAEWRG
                     LGTIPDSGLKVREEYARFDARRALQIDPGPTREHRACRCGEVLRGIIKPPECPLYGRA
                     CTPETPVGPCMVSSEGTCSTWFQFAGVE"
     gene            complement(2066..2302)
                     /locus_tag="VHS06_11735"
     CDS             complement(2066..2302)
                     /locus_tag="VHS06_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX2988827.1"
                     /translation="MCLAVPARIMEIHGNKATVEMAGVVRETSIMMLPGARVGDYVII
                     HAGYAIEKLDEEEAQRTVELFKMMESLGAEETKN"
BASE COUNT          402 a          793 c          713 g          460 t
ORIGIN      
        1 ggggccgcga gcacgcgccc gatgatccgt gcttcctccc cataacgggt tcgacgcatg
       61 gcggacagcg cagcctccgc ctcctgctcc ggcaggatgg ccaccagctt tccctcgttg
      121 gccacgtaga gggggtcgta gcccagcatc tcgcaggctg cctggactgc cggccgcacc
      181 ggtatctgtc cctcttcaat ctggattgcc acgccggatt gggccgcgaa ctcgttgaga
      241 gtcgtggcga gcccgcccct cgttggatcc ctcatggcgt ggatgtgtgg ggcaacctcc
      301 agcatcgcgg ccaccatcct gttcaggggc gcgacgtcgc tggccacggg cgagccgaac
      361 tccaacccct cgcgcttgct gaggaccgct atgccgtgat cccctatggt gccggagagc
      421 aggacgacgt cgccctcgca ggcgttgctg cccgaaatgt cgatgccttc gggcactatg
      481 cccacgcctg tggtagtgac gtacagcccg tcggcgctcc cctgctccac aaccttcgtg
      541 tcgccggtga ccacgaacac ccctgcttcc tccgcggcca acttcatgga ttcgaccacc
      601 cgtcgcaggt cggcgatggg cagcccctcc tccagcacga agcccgcgga caggtagagg
      661 ggcgttgccc ccaccaccga caggtcgttc acggtgccac agatggccag ccggccgatg
      721 tccccaccgt cgaagaagag cggcttcacc acgtgggagt cggtggtcat ggcgaggcgg
      781 ggggctttgc cctcaccccg accctctccc agagggagag ggggattcgc agcctcccga
      841 gggagaggga gaattgctga atcatccatg cgggccaggg ctgggttgcc gaaggccgga
      901 acgaagactt cctggatcag ctcggcactc agctttccgc cgctgccatg ggcaaggagt
      961 aatctatcgc ctctcattcc acccccgcaa attggaacca ggttgagcag gtcccctcac
     1021 tggagaccat gcatgggccg accggcgtct caggcgtgca ggctcggccg tagaggggac
     1081 attcgggcgg ctttatgata cccctgagca cctctccgca gcggcacgcc cggtgctctc
     1141 gagtcggtcc cgggtctatc tggagtgccc tcctggcatc gaatcgggcg tactcctctc
     1201 gaaccttcag cccggagtcc ggtatcgtcc ccaatcctcg ccactcagcg tccgtgggtt
     1261 ggaacacccg gtagagcgct tccagcgcgg cggggtttcc ctttggtcgc acgaccctac
     1321 tgtactggat ctccacctcg gctcgaccct ccacccgctg ggccagcagc atgtctatgg
     1381 attgcagcac atccagcggt tcgaacccgg cgatgacgca ggggacgccg taatcctcgg
     1441 ccaggaagcg gtacggctcg gagccgatga tggtgctgac gtgccccggg cagaggaatc
     1501 cgtcgatgcg gacctcaccg gaatccacca gggccttcat ggggggaggc atcaccttgt
     1561 ggacggagaa gacgaagtag ttgtttatct cggcctcctc cgcctccaga accgagcagg
     1621 ctatggttgg cgctgtggtt tcgaagccga tcccgaagaa gacgaccttc ttggaggggt
     1681 tcgatcgggc gatctgcagg gcatccaggg tggagtagac cacacgcacg tctcccccct
     1741 ctgccctcac ctgttgcagg ctggagtagg aacccgggac ttttagcatg tctccgaagg
     1801 tggtgaggat ggtgtcggga tgctttgccg cggcgatggc ccagtcgatg tcgccgtttg
     1861 ccgtcacgca caccgggcaa cccggtcctg agatcaggtt tacattctcc ggtagcagtt
     1921 gccggatccc gtgtcggaat atggacaccg tatgagtgcc gcacacctcc atgaagttga
     1981 cggggatggg gggtcggcgg cgaatccgct ctaccagtct ggcggccatc tcactatctc
     2041 ggaactcatc caggtatttc atgggctagt tcttcgtttc ctcggcgccg agggactcca
     2101 tcatcttgaa gagttccacc gtgcgctgag cctcttcctc gtccaacttc tctatggcgt
     2161 agccagcgtg gattatgacg tagtccccca cccgcgcgcc gggcagcatc atgatgctcg
     2221 tttcgcgcac gacaccggcc atctccactg tggccttatt cccgtgaatc tccatgatcc
     2281 tcgccggcac agcaagacac atcttctatc ctccagcact cagcactcgg tttgccacca
     2341 tggcctggcc cagggcgatc cccccaat
//
LOCUS       DATALS010000528         3203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_125798, whole genome shotgun sequence.
ACCESSION   DATALS010000528 DATALS010000000
VERSION     DATALS010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125798"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..2201)
                     /gene="feoB"
                     /locus_tag="VHS06_11740"
     CDS             complement(354..2201)
                     /gene="feoB"
                     /locus_tag="VHS06_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX2988828.1"
                     /translation="MAANCCATIQVEAPPSAKRIVLAGNPNTGKSVFFNALTGLYVDV
                     SNFPGTTVDISQGRWGDELVIDTPGIYGVASFNDEERVARDIILTADAVVNVVDAVHL
                     ERDLFLTQQLIDMGLPMVVALNMMDEAEAQGLKIDHLQLQELLGVPVVPAAAVRNRGI
                     SELKETIPAARTGNVDPWLRKKMVEWYGDDLPTPQRLLAAEGDEDVALSSGLPMGGSR
                     EELYVQRRRRVNEVVDRVVADTREERGFSETLGRWMVNPVTGIPILMGVLAAVYYLIG
                     DLVAQQVVGFTEDTLMKGMYEPFVQGLVSRFADLSSPIGTILAGEFGLLTMTMTYLAG
                     LLLPLVVGFYLVLALMEDSGYLPRLATLVDRALTGFGLNGRAIVPLILGLGCVTMATM
                     TTRILGSQRERRIAVALLGLTIPCSAQLAVITAMLAQLGPRYVLLYTTMVVAILVAAG
                     TILNRLMPGKSTGLLIDLPPLRVPQPMNVLRKTAGKVKMFLMDAGPLFAFGSLLITLL
                     QLSGGLQGIQDALTPLTVGWLQLPAESSTAFVMGFVRRDFGAAGLLEMQLTPIQTVVA
                     LVTLTLFVPCIASAMMLYKERGWREASVVWGGCLVIAFVVGGLLSQILI"
     gene            complement(2203..2421)
                     /locus_tag="VHS06_11745"
     CDS             complement(2203..2421)
                     /locus_tag="VHS06_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX2988829.1"
                     /translation="MTLDMVHRGERVRILNIGDPQVRIQAIRFGIGEGSDVQCAEKLP
                     SGPIVLRRGRQEIAIGRGIARNIAVEAY"
     gene            complement(2569..3024)
                     /locus_tag="VHS06_11750"
     CDS             complement(2569..3024)
                     /locus_tag="VHS06_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX2988830.1"
                     /translation="MGSAAELSTTARSAINSSGRRITPQRMALLEIIQQSSEHLDADE
                     IYRRARERGERVSLSTVYRTLDLLKELQLVDELHLWDEHHHYESRTNREHCHLLCHRC
                     GGVQELSGAVVDQMKSLASQQLGFQIERAQLDFVGVCEECGKAAPSSQR"
BASE COUNT          651 a         1023 c          974 g          555 t
ORIGIN      
        1 cctgggaaaa gccgcggcga agccaggctc gccgctcctc caggcggttt gggtacatcc
       61 tgtcatagtg gcggccgagg tggttgtcgg ccgtcaccac cacacgaaca gcgatggcac
      121 tactccctcg tttgctagat agattctagc atgcgggagg ggctaccggc cgaggggaca
      181 ccccttacac acggcacaac tcatcctggg aagcttcgtc cagcagcggg ggcactgaac
      241 cacgagggga tcgctaaccg cgttgccaca ctgggggcag agtcgcagtt cgcaggagtt
      301 cctctgggcg gcctcggccg atctggtagc agtctcgttc atcgtcgaac tccttagatc
      361 aggatctggg aaaggaggcc acccaccacg aaggcgatca ccaggcaacc gccccacacg
      421 accgatgcct cccgccatcc ccgttcctta tagagcatca tcgccgatgc gatgcagggg
      481 acgaagaggg taagggtcac aagggcaact accgtctgga tcggggtgag ctgcatctcc
      541 agcagcccag ccgccccgaa gtcgcggcga acgaagccca ttacgaaagc ggtagaggat
      601 tcggcaggca actgcagcca gccgacagtc aacggcgtga gagcgtcctg gatcccctgg
      661 aggccgccgc tcaactgcag aagggttatc agcagggagc cgaaggcgaa gagagggccg
      721 gcatccatca ggaacatctt caccttgccg gcggtcttcc ggagcacatt catcggctgc
      781 ggcactcgga ggggaggcag atcgatcagc agccccgtgg acttgcccgg catcagccgg
      841 ttgaggatcg tcccggcggc caccaggata gccaccacca tggtggtgta gagaagcacg
      901 tagcgggggc cgagttgggc gagcatcgcg gtgatcacgg caagctgtgc cgagcacggg
      961 atcgtaagcc cgagcagcgc cacggcgatc cgacgctccc gctgagaccc caggatgcgg
     1021 gtagtcatgg tggccatcgt cacgcagccc agccccagga tcaacggcac gatggctcgc
     1081 ccgttcagcc cgaaaccggt gagggcccta tccaccagag tggccagcct gggtagataa
     1141 ccggagtcct ccatcagagc cagcaccagg tagaagccga cgacgagggg gagaaggagt
     1201 cccgccaaat aggtcatggt catggtgagc aggccgaact cgcctgccag gatcgtcccg
     1261 atgggagagc tgagatcggc gaagcggcta accagcccct ggacgaaggg ctcgtacatc
     1321 cccttcatca gcgtgtcttc ggtgaagcca accacctgct gtgccaccag atcgccgatc
     1381 aggtagtaga cggcggccag aacccccatc aggatcggga tgccggtgac ggggttcacc
     1441 atccagcggc ccagcgtctc ggagaagccg cgctcctccc tggtatcggc cacaaccctg
     1501 tccacgacct cgttcacccg ccggcggcgc tggacataca actcctcacg ggagccgccc
     1561 atgggaaggc cggacgacag ggcgacatcc tcatccccct ccgccgctag caaccgctga
     1621 ggcgtcggca gatcgtctcc gtaccattcc accatcttct tccgcagcca ggggtccacg
     1681 ttgccggtcc gcgcggcggg gatagtctct ttcaactcag agatgccgcg gtttcgcaca
     1741 gccgcggcgg gaacgaccgg gacccccagc aactcttgaa gctgcaggtg atcgatcttg
     1801 agtccctgcg cctccgcctc gtccatcatg ttcagggcta ccaccatggg gagtcccatg
     1861 tctatcaact gctgagtgag gaagaggtcg cgctccaggt ggacggcgtc caccacattg
     1921 accactgcgt cggccgtgag gattatgtcg cgagccaccc tctcctcgtc gttgaaggaa
     1981 gcgactccgt agatgcctgg cgtatcgatc accaactcgt cgccccagcg cccctggctg
     2041 atatcgacgg tggtgcctgg gaagttggac acgtccacgt acagcccggt gagggcgttg
     2101 aagaagaccg acttaccggt attggggttg ccggcgagga cgattcgctt tgcggatggt
     2161 ggcgcctcca cctggatggt ggcgcagcag ttggcggcca tattagtaag cctcgacggc
     2221 gatattcctc gcgatacctc ggccgatggc gatctcctgg cgtccgcgac gcaggacgat
     2281 ggggccggag gggagtttct cggcgcactg gacgtcggag ccctcgccga tgccgaaacg
     2341 gatggcctgg atcctaacct gggggtcgcc gatgttgagg atgcgtacac gttcgccgcg
     2401 atggaccata tccagagtca ttgtggtttc ctccttgata tcggcgggga tcgccgccct
     2461 ggcgccccgc ggactttctg gccgccgccc tttgctgcga ttgacactca ttcgcagcaa
     2521 ctagaacgaa caagcctgag gtactggtta ccgaaaaaaa agagacggtt accgctgaga
     2581 gctaggagcg gccttcccgc actcctcaca cacccccacg aagtccaatt gggctcgctc
     2641 tatctgaaag cccaactgct ggctagccaa cgacttcatc tgatcgacca cggcaccgga
     2701 gagttcctgg accccgccgc agcggtggca gaggagatgg cagtgctcac gattggtgcg
     2761 ggactcgtaa tggtggtgct cgtcccacag gtggagttca tccaccaact gcagttcctt
     2821 cagaaggtcg agggtacgat agacggtaga gaggctcact cgctcgccgc gctctctggc
     2881 ccggcgatag atctcatcgg cgtcgaggtg ctcgctggac tgctggatga tctccaacag
     2941 agccatgcgc tggggggtga tcctgcgccc cgacgaattg atggcgctcc gcgccgtggt
     3001 gctcaactcc gctgccgacc ccatcgtcgt ccaccctcta cttgcaaatc gttcgcaatc
     3061 gcaatagtac cacttcgcac gatcctgtca aggggttccg ccacaactcc attaaaccaa
     3121 aactccatcc taaaattaac taaaactcca tccacaacct gcccagcatc ttgcctggcc
     3181 gaagggatgg cgacagagca caa
//
LOCUS       DATALS010000529        18078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_78589,
            whole genome shotgun sequence.
ACCESSION   DATALS010000529 DATALS010000000
VERSION     DATALS010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 18078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18078
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78589"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VHS06_11755"
     CDS             <1..411
                     /locus_tag="VHS06_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEX2988831.1"
                     /translation="KAIAREAGARSKVAVAARQEGIDPVGSCVGMRGVRIQNIVNELG
                     GEKIDVVQWSPDPAAFVANALSPAQVISVDIDEEDKTATVMVPERQLSLAIGKEGQNA
                     RLAAKLTGWRIDIKAASSEPVSHPADEAAPESAL"
     gene            471..794
                     /locus_tag="VHS06_11760"
     CDS             471..794
                     /locus_tag="VHS06_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX2988832.1"
                     /translation="MLRQKHIPQRTCVGCGQLQAKRQMVRVVRTVEGPVVVDPTGKRT
                     GRGAYLCKKTACWDMAMQKSSLNRALKTEVSSEDREELIRYSRLFGAESSTQSAPEAD
                     GEAGS"
     gene            988..2727
                     /gene="infB"
                     /locus_tag="VHS06_11765"
                     /pseudo
     CDS             988..2727
                     /gene="infB"
                     /locus_tag="VHS06_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
     gene            2806..3159
                     /gene="rbfA"
                     /locus_tag="VHS06_11770"
     CDS             2806..3159
                     /gene="rbfA"
                     /locus_tag="VHS06_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX2988833.1"
                     /translation="MSRRMERVNEVIKEEVAVLLQRQVQDPRLSTMVSVTGVETSSDL
                     RNATIFVSVLGSEEEVQNAMAALRHASGYLRRELAGRIRMKQVPDLSFKLDTSIGQGA
                     RVLELLREIHKEEEG"
     gene            3169..4197
                     /locus_tag="VHS06_11775"
     CDS             3169..4197
                     /locus_tag="VHS06_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX2988834.1"
                     /translation="MEPRYTPVLLPLLKTVRKVVVSCHADPDGDAVAGMLALSRSLKK
                     ARWDVRLISPGPVPRAYRFLPGWEDVEVYRQERIEMPDGIAVRDAFRGAEAIICLDCS
                     DLSRLGPLYSGNTRKFESTPVVNIDHHPTNSRFGRVNLVDTSAAATCEILSVLMENED
                     LPVDEEVATSLMMGILTDTLGFRTPATSARTLRVAAALMERGASLSRISERIFNSRSP
                     RTLQLWGRVLSRTQVDGRLVWSEITSEMLAECGATLEDADMLVDFIAGVPQTDAAFLF
                     SEQGGMVRVSMRTSEALNAATVAKRFGGGGHARAAGCTLEGSMSDVETRLMNEARERL
                     GMAAEGDR"
     gene            4187..5086
                     /gene="truB"
                     /locus_tag="VHS06_11780"
     CDS             4187..5086
                     /gene="truB"
                     /locus_tag="VHS06_11780"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX2988835.1"
                     /translation="MIARTGTSSMSGFLNVCKPYGWTSHDVVQLVRKLSGTRRVGHSG
                     TLDPAATGVLVVAVGKATRLVDYLGDQDKSYCGDIVLGAATNTDDAEGSVLYARDPSG
                     ILLSDVSASLSGFLGEIEQLPPQFSAVKLAGQKAYEIARRGGEAKLTPRRVTIKGLAV
                     VDWKPPTLSVVVHCSKGTYIRSLARDLGERLGVGAHLGALVRLTNGPFDCGDAVGVEE
                     LRFAAEFGYLGDMLWPPDLAVAHRPALIVSEQHAMDMLGGRRWRASSPTFEGPLRVYS
                     EDGSFLGLAELQEGAWQPKLVLT"
     gene            5226..6143
                     /locus_tag="VHS06_11785"
     CDS             5226..6143
                     /locus_tag="VHS06_11785"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX2988836.1"
                     /translation="MTKNGAPEVVATIGVFDGVHVGHQDLIGKVVERARRLGVSSCCV
                     TFSPHPQEVLRPEVELSHLCTLKDRLDLIKGLGVDDVLVIEFTRALAQMLPEEFVHLL
                     LRRHRIRELWVGSDFALGRNRTGTPDKLAEIGRVEGFEVRPFDRVERFGAPVSSSRIR
                     GLLVEGRVEETAQLLGRPYRLSGVVGQGDRRGRTLGFATANLTQMDNLCVPGNGVYAV
                     WGRTRAGLVYPCVTNVGIHPTFGGTARQVEVHLLDFEGNLYGDSLNVDFVGRLRDELR
                     FDSVEELIGQMKSDVVDARQLLAGTPPVA"
     gene            6273..7034
                     /locus_tag="VHS06_11790"
     CDS             6273..7034
                     /locus_tag="VHS06_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX2988837.1"
                     /translation="MASIIAISNQKGGVGKTTTTANLGGALFYMGRKVLMVDMDPQSN
                     LTSAFGKSEQTETSIAEALLDRKVPLPVVEVAAQVDARLDMVPSTLALASAEAALMNK
                     LGRELRLRDQLAPMGTTYDFILIDTPPSLGLLTINALVAAERVVIPTEARLFSLQGLQ
                     MMQESIEEILYLNPRLQVLGIVLSKLDRRLKEEKLVSSYLRERWGAQVFETEIGTNSK
                     ILEAASSGTSVFNYAGAEKAAESYLSLAREVLGRG"
     gene            7027..8427
                     /gene="ftsZ"
                     /locus_tag="VHS06_11795"
     CDS             7027..8427
                     /gene="ftsZ"
                     /locus_tag="VHS06_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX2988838.1"
                     /translation="MAKMRGGFDPDRLPEQTRSSPIIGKIVSEEIETEAKGFTEIKIV
                     GVGGGGNNALNRMIEAGIRGVDFVAVNTDAQALEMSLATHKIPIGGKAMKRLGAGGNP
                     MVGERAAEISRDQLEETLAGADMVFITAGMGGGTGTGASPVIAEIAKKNRSLVVGVVT
                     LPFTFEGRQRRKTAEQGLEVLRDRVDAIIAIPNDRLLQMANKKTGVLEAFKLADNILR
                     QGVQGISDLITVTGLINLDFADVKTIMSGAGTALMAIGEAKGPGRALLAAKMAIESPL
                     LDVSITGATGLLINVTGGPDMSLMEVSDAAAAIADAASPNANIIMGAVVNPRPQPEIQ
                     VTLIATGFGGAEIKKPDIKVSEEFAARLERQIGQQQQAHQPRVEPTPRMDPAPRIEEQ
                     SRGQRTPDREPWETPVPLDREGDEDWTVRDVRGPGRESWEPMDPGETRNSRAPREPDR
                     NDLDIPAFLRRKRPQQ"
     gene            complement(8482..10545)
                     /gene="fusA"
                     /locus_tag="VHS06_11800"
     CDS             complement(8482..10545)
                     /gene="fusA"
                     /locus_tag="VHS06_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX2988839.1"
                     /translation="MKEYQAEKIRNVGLFSHGGAGKTSLAEAMLFQSGAITRLGKVEE
                     GSTTTDYDPDEIKRHISVSTALAPCEWQGHKLNVLDTPGYADFVGEVVQTMRVVDGAL
                     ILVDAVSGLEVGTETAWQEAEKNGVPRMFFVNKMERENANFDTVLQALRTRFGKGVVA
                     LQIPIGSQQNFSGVVDLISMTVIGEGKEPPKEVTGDLRDAAQAQRDALIETVVEVDDE
                     LINKYLEGEEISVEEIQRALKSGVQSRSLFPVMCGSALANRAITPVLDAMVSYVPSPL
                     ESTVRTTNGDVPAKELAGKNQLAALVFKTVSDPYVGRLNYFRVYSGAVQSDSHIWNPN
                     RGREERIGQLYTLVGKRQDPVPKIALGDIGAVAKLQETSTGDTMTTKDAGIQLVGIEF
                     PDPVFSAAIAPKTKADLDKLGTAMARMTEEDPTIRVEKNAETGDLLMSGMGESHIDIT
                     AERMKRKFGVDVTIGIPKVPYRETITSSAKAQGRYKKQTGGHGQFGDTWLELEPLPRD
                     QEFEFQDRVVGGAVPKNFIPAVEKGVREALQEGILAGFPVVAVRATLYDGSYHPVDSS
                     EMSFKIAASMGFKKAAELAKPVLLEPIMEMAITIPDAYTGDVIGDLNSKRAQVLGMIP
                     EDGFTTIQARAPYAEVRRYATDLRSITQARGKFTMKFSGYDEVPAHAAQAVIAEAKKE
                     KEQEK"
     gene            10795..11541
                     /locus_tag="VHS06_11805"
     CDS             10795..11541
                     /locus_tag="VHS06_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2988840.1"
                     /translation="MSGHSKWAQIKRQKGVNDNKRGQVFTKLGREITVAARDGGGDPN
                     ANFRLRLAVQHARENNMPMDNIERAIKRGTGGGEGAALEEITYEGYGVGGAAILVEAM
                     TDNRNRAAADVRSVFTRHGGSLGETGCVNWLFDSRGIISIDVGKQDPDELALLAIDAG
                     ADDVKVEDGSLEVYTAPGDLESVRQALEANKLTITNAEVSMIPKTTVELDPKGTVTVL
                     KMVEKLEDLDDVQAVYSNIEVSDEALAEFS"
     gene            11563..12060
                     /gene="ruvC"
                     /locus_tag="VHS06_11810"
     CDS             11563..12060
                     /gene="ruvC"
                     /locus_tag="VHS06_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX2988841.1"
                     /translation="MVVLGIDPGTAILGYGVVALEGDSLSALDFGVLTTPSSLPLTSR
                     LLTLFDGLTEVISRNNPSEVAVEQLFFARNVQSALAVGHARGVALLSAARHCLPVSEY
                     TPQQVKQAVAGYGRATKMQVQSMVQIMLSLQSIPQPDDAADALAIAICHARWNELTGG
                     GRVVL"
     gene            12057..12641
                     /gene="ruvA"
                     /locus_tag="VHS06_11815"
     CDS             12057..12641
                     /gene="ruvA"
                     /locus_tag="VHS06_11815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX2988842.1"
                     /translation="MIAGLHGTVEEIGGDFLLINVGGVVYQTYASSATLSSVSSPGAV
                     IHLHTHLQVREDGMTLFGFASREELRMFQLLLSVSGVGPRIALALLSTMSVEDLIAAI
                     AAEDTARLSTVTGVGKRTAARIALELKAKVGQAQVAAMAASPGNSAQLLDTLTALGYS
                     STEAASAVRAIPHLQTMELEDALREALRWLSSAR"
     gene            12768..13286
                     /locus_tag="VHS06_11820"
     CDS             12768..13286
                     /locus_tag="VHS06_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988843.1"
                     /translation="MLAIGAVGGVLIAFVAAMVRRGGISEMGAPVFGGYGQETGMAGL
                     NRVRADVETIKREVESMEQSVGSMHRELLSRMDDIRSQLGGAGAPGGAPPASRGTEKT
                     TGTRTRRTTGAAAGGPQGATTAPERPARRRTRKSEAAVAAEAFAQPPTSRSQGKTQQT
                     SGGGQTQGQSGR"
     gene            13487..14071
                     /locus_tag="VHS06_11825"
     CDS             13487..14071
                     /locus_tag="VHS06_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988844.1"
                     /translation="MAERGRRKAEVEDYEVAEPAEVEVEEAVAPVAEEAEKPVEAAEE
                     ARYFVDPQWYEERGLVFSTVAQARLCYSCASKLGTEVEERYPVIDPKTKRVTFEFRRV
                     PYAANPLPIIRDCCSRARDYITNETPLLEAVFRVFLANGNQPMTASTVREHLLTYLPE
                     MAALRSDYPVELLERLIRGDKNYGLREHTVPVSA"
     gene            complement(14197..15063)
                     /gene="galU"
                     /locus_tag="VHS06_11830"
     CDS             complement(14197..15063)
                     /gene="galU"
                     /locus_tag="VHS06_11830"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX2988845.1"
                     /translation="MKKVRKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPVIQYIVEE
                     AVASGIEQIVIITGQSKRAIEDHFDYPFELAHRLRENNKIDELHEVERLSELANFVYV
                     RQKQPLGNGHAVLVAREVIGDEPFAVLWGDDLVDAQVPCLQQLISVYEQYGTSVMAVM
                     RVAKENISKYGVIDPRPVSERIHEVIDLVEKPSQDSAPSDLAAVKGFVLTPEIFEELE
                     RTPAGKGGEIWLSDAIKSLMRRQKVFALEFDGKRYDAGNKLEFLQATVDFALKRPDLS
                     DDFRAYLKGLAL"
     gene            complement(15119..16285)
                     /locus_tag="VHS06_11835"
     CDS             complement(15119..16285)
                     /locus_tag="VHS06_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2988846.1"
                     /translation="MQRDPWLKTLIILLVLIAASYLAGLVWTLAVRFADILLLLVLAW
                     VLAFVLEPVAYALETRYRLNRAVAVGIVYLGLLVVLSLFTILLIPVIALQVTQIGINL
                     PIYVAAAGDWLTSLQYQAAARGLDVNATRILDYNQIARYVESMGPPIVNNALALATGV
                     ASLLFDLILVLMFSFYVALDGSRFATAVLKAIPIDRRDDVNYLIYSTHQAFGGFIRGQ
                     LIQALIYGLVTVGVMMAADLSYTAAASVFGMAIMLVPVIGPMLALIPPVVIALLIHPG
                     RAWWVFIILLLMQQVILNVLGPRFVSRSVGMHPLLVLVSLLVGSKLAGMWGALFAVPV
                     AGVIVAMVSFYRMTVEERKVHMEEKVDEGSWQATPSDEQPEMEEPHPALSNDNR"
     gene            16378..16803
                     /locus_tag="VHS06_11840"
     CDS             16378..16803
                     /locus_tag="VHS06_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2988847.1"
                     /translation="MIRYRILVADPNPAVRKLLSMVFDSERCELSFASDGEEALGMAI
                     VAPPDLVIAELRLDRLDGIALCERLKEHSRTVKTKLLFLTTCASEWEMRRARRAKADG
                     YMTKPFSPMALLRQADELLEGKPVDKGEDLSCRLLDKGS"
     gene            complement(16813..17451)
                     /locus_tag="VHS06_11845"
     CDS             complement(16813..17451)
                     /locus_tag="VHS06_11845"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX2988848.1"
                     /translation="MERDQLAIDRLGRIWAIVEYIAEHPGTSRQKLAKRYSLSERQVQ
                     EDLRVIREELHFPLVRHKGYRFSAGDDHVVLDFSFSEARALISALKIATTDRTVPQEP
                     LRSLLAKLPAVFPLHLQPLIRKSLESLGNPEPALEDKLFLVLTEALFERRPVRLHLSA
                     RAAGGIPDPVVEPSLLMPYLGTWYLVGRCHQKKRVMMFKIENIEAVTLASKL"
     gene            complement(17697..17770)
                     /locus_tag="VHS06_11850"
     tRNA            complement(17697..17770)
                     /locus_tag="VHS06_11850"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17734..17736),aa:Arg,seq:gcg)
     gene            complement(17778..17870)
                     /locus_tag="VHS06_11855"
     tRNA            complement(17778..17870)
                     /locus_tag="VHS06_11855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17833..17835),aa:Ser,seq:gct)
     gene            complement(17917..17999)
                     /locus_tag="VHS06_11860"
     tRNA            complement(17917..17999)
                     /locus_tag="VHS06_11860"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17963..17965),aa:Ser,seq:tga)
BASE COUNT         3564 a         5092 c         5891 g         3531 t
ORIGIN      
        1 aaggcgatcg ctcgtgaggc gggcgctcgc tccaaggtcg ccgttgcagc ccggcaggag
       61 gggatcgacc ctgttgggtc gtgcgtgggg atgcgcggag tgcgcattca gaacattgtg
      121 aatgagctgg gcggcgagaa gatcgacgtg gtgcagtgga gcccggatcc ggctgctttc
      181 gtggccaacg cgctgagccc tgcgcaggtg atcagcgtcg atatcgacga ggaagacaag
      241 actgcgacgg tgatggtccc ggagcgccag ctgtcgctgg cgatcgggaa ggaaggacag
      301 aacgcccggc tggctgcaaa gctgacgggc tggcgtattg acataaaagc ggcgagcagt
      361 gagccggtgt ctcatccggc ggacgaagcc gccccagaga gtgcccttta gggaggatta
      421 ttcctggcgg ggcgagtggt gactgacttg ccctcgacgg gaggagagcc atgttgcggc
      481 agaagcacat tccccagagg acctgtgtcg gctgtggcca gcttcaggcc aagcggcaga
      541 tggtgagagt ggttcgaacg gttgagggcc ctgtcgtcgt ggaccctacc ggaaaacgga
      601 ccgggcgggg cgcttatctg tgcaagaaga ccgcctgctg ggacatggcc atgcagaaat
      661 cgtccctgaa tcgtgctctc aagacggagg tctcctccga ggacagggag gagctaatca
      721 ggtatagtcg gctgttcggc gccgagtctt caactcagtc tgctcctgaa gcggatggcg
      781 aggcgggcag ttgagcgaaa ggaggagcag tccatgagtc ctggaccgtt caggaaatct
      841 tcaggcggtg gtagaggaga tagcagaagg cctcagggta acagacccag gccaggcgga
      901 ggagggggtg gtcaaaggcc accctttgtg cgctctacgc ccgctaacga cggaggggta
      961 gcaactgcga cccgtccgga agagcagaag acaatcgaat tgccggacag catcgccgtt
     1021 aaggatctgg ccgacctgat gaagattagc cccgttcaga tcatcaagga gttgatgaag
     1081 aacgggatca tcgcgaatat caaccagcag atcgatcggg atgcggccac agtggtggcc
     1141 accgatctgg gctacgagat caaggaaccc gcgcccgtgg atcgatccgc ggcagccatc
     1201 cctgaagagg aagaggagga tccctccctt cttcaaccga ggccgccggt cgttacgatc
     1261 atggggcacg tagaccatgg caagaccagc cttctggatg tgatccgcca gactaacgtg
     1321 accgctcaag aggccggcgg tatcacacag cagatcggtg cctaccaggt tgagctgcac
     1381 ggccagaaga ttaccttcct ggacacgccc ggccacgagg cgttcaccgc gatgagggcg
     1441 cgaggtgccc aggcaaccga tatcgcggtc ctcgtggttg cggcagacga cggcgtgatg
     1501 ccgcagaccc gagaggctat cgaccacgcc cgggcggcaa acgtcccgat catcgtcgcc
     1561 atcaacaaga tcgacaaggc cgacgcgaac ccggataggg tgaagcagca gctgacggag
     1621 atcggcctgg tgccggagga atatggtggc cagaccatca tggtgccggt ttcggctcgc
     1681 cgcaaggagg ggatcgatag cctcctggag atgatcctgc tggtatcgga gatggccgag
     1741 ttgaaggcca accccaatcg aagctcgatg ggtgtggtca tcgaggcgaa gctggacagg
     1801 gctcgaggtc cggtggcgac catccttgtc aagaccggga ctttgaaggt cggcgacaat
     1861 gtggtggccg gcaacaacta cggcaaggta aaggcgctgt tcgatgagaa gggccggagg
     1921 ctccgcaagg ccggtccgtc catgccggcc gagttgttgg gcatgggcag cgttcccacc
     1981 gctggggacg tgtggcgagt agttgccgac gagaagaccg cccgggcgat ggcgggcgcc
     2041 aaggctgcag agcccgtggt ggagagtgtt tcggctccgg tgactctgga cgatgtgttc
     2101 aagcagatcc aggccggaca ggtgaaggag cttggtgtcg tcttgaagac ggacatccag
     2161 ggttcggtcg agccgatcgt cgtttcgctg aaccggcttc aggctgagga tgttcgggtg
     2221 cgagtgatcc actccggcac cggaagcatc acggagtctg atgtcctgct ggcgtcggcc
     2281 tcaaatgcgg tcatcgtcgg tttcaatact cgtccggagc caggggcgaa gcaccttgcc
     2341 gaggagaacg ggatcgacat ccgcttctac gacgtgatct acaagctggt ggaagacgtc
     2401 cagaaggcgc tcaccggttt gctggagccg aagtacgagg aggtcgtcga gggtcacgct
     2461 gaggtccggc aggtcttcaa gatgggcaag agcgagactg tggctggatc catggttacc
     2521 gacggcaagg tctcgcgcaa ttccaaggcg agactcattc gcggcggcag cgtggccttc
     2581 gaggggcggc tggcaagcct gcgtcgcttc aaggaggacg ccaaagaggt ggctgcaggt
     2641 tacgaatgcg gcattcacct ggatggcttc agcgatctcc aggtgggcga cgtgttggag
     2701 ttctacagga tggagcgggt gagctaacac ccgagtatcg taggggcgac gcctgcgtcg
     2761 cccctatatt cgttcagtag ctgttttgaa ggtgcggtga ctgtcatgag caggcggatg
     2821 gagcgggtca acgaggtgat caaggaggag gttgccgtcc tccttcagcg ccaggtccag
     2881 gatccgcgcc ttagcactat ggtgagtgtt accggggtgg agaccagttc ggatttgcgg
     2941 aacgccacca tatttgtgag tgtgctgggt agcgaggagg aggtgcagaa tgcgatggct
     3001 gccctccgac atgcctccgg ctacctgagg cgagagttgg cgggccgtat caggatgaag
     3061 caggtccctg atttgtcgtt caagctggat acctcgatcg gccaaggcgc gcgcgtgctc
     3121 gagcttctgc gggagatcca caaggaagaa gaggggtagg gcgtcagggt ggagcctaga
     3181 tacacaccgg tgttgctgcc gctgttgaag accgtcagaa aggtcgtggt gagctgccac
     3241 gcggaccccg atggcgacgc cgtagctggg atgttggccc tctcgaggtc tttgaagaag
     3301 gcccgatggg atgttcggct catcagcccc gggccggttc cgcgggcata tcgttttctt
     3361 cccggatggg aggatgtcga ggtgtatcga caggaacgca tcgaaatgcc cgacggtatc
     3421 gccgttaggg atgcgttccg gggggccgaa gcgatcatct gccttgattg cagtgacttg
     3481 tcgcggctcg gtccactgta cagcggcaac acccgaaagt tcgagagtac cccggtcgta
     3541 aacatcgacc accaccccac gaacagccgc tttggcaggg tgaatctcgt ggatacatcg
     3601 gcagccgcaa cgtgcgagat cttgagtgtg ctgatggaga acgaggacct acccgtggac
     3661 gaggaggtgg cgacctccct catgatgggc attctgaccg atacgctggg cttccggact
     3721 cctgcgacct ctgcccgtac tctgcgtgtc gctgccgcct tgatggagag gggcgcctcc
     3781 ctctctcgca taagcgagcg gatattcaac agtcggagcc cccggaccct tcagctatgg
     3841 gggcgggtgc tcagtcgcac ccaagtcgac ggccgactgg tatggtcgga gatcaccagc
     3901 gagatgctgg ccgagtgcgg ggcgacgctg gaggatgccg atatgctggt tgacttcatc
     3961 gccggagtgc ctcagaccga tgccgcgttc ctgttcagtg agcagggtgg catggtgcgg
     4021 gtgagcatgc ggacctccga ggccctgaat gccgcaacgg tggccaaacg gtttggcgga
     4081 ggtggacacg cacgagcggc agggtgcacc ctagagggct ccatgagcga cgtggagacc
     4141 cggctcatga acgaggcgcg cgagcgcctg gggatggcgg cggagggtga tcgctagaac
     4201 cggcacctcc tccatgagcg gcttcctcaa cgtctgcaag ccttacggct ggacatctca
     4261 cgatgtggtg cagttggtga ggaagctgtc cggcacccgt cgcgtgggcc acagcgggac
     4321 tctggatccg gcagccacgg gcgtgctcgt ggtggcagtg ggcaaggcaa ctcgccttgt
     4381 ggattacctt ggcgaccagg acaagagcta ttgtggggat atcgttctgg gtgcggccac
     4441 caacaccgac gacgccgagg ggagcgtgct ttacgcgaga gatccatccg gcatcctgct
     4501 atccgacgtt tccgcctcgc tgtccggctt cctcggcgaa atcgaacagc ttcctcccca
     4561 gttctctgcg gtgaagctgg cggggcagaa ggcgtacgag atagccagac gtgggggcga
     4621 ggcgaagttg accccacgaa gggtgacgat aaagggactc gcggttgtgg attggaagcc
     4681 gcctactctc agcgttgtgg tgcactgctc caaggggacc tacattcgaa gcctcgctcg
     4741 ggatctggga gagaggctgg gtgtgggggc gcatctgggt gctctggtga ggctcaccaa
     4801 tgggccgttc gactgtggcg atgcggttgg agtcgaggag ttgaggtttg ccgcggagtt
     4861 cgggtacctg ggcgacatgc tttggccacc cgatcttgct gtagcccacc gtcccgcact
     4921 gatagtgtcc gagcaacatg ctatggacat gttgggtggt cgcaggtggc gcgccagttc
     4981 ccccactttc gagggcccct tgcgagtcta ctccgaggac gggagtttcc tcgggcttgc
     5041 tgagcttcaa gagggagcgt ggcagcccaa gctcgtcctg acgtagtggg acacggtggt
     5101 cttgatttct aggagcctgg gtcgtccctg acccaggcgg cccaacggaa cgaccgtcgg
     5161 ggtcagggac gaccccgact cctagatgcg aggccagcgc gtagtctttt ccaggagtga
     5221 acgctttgac caagaacggc gcgcctgagg ttgtggctac catcggggtg ttcgatggag
     5281 tccacgtagg gcaccaggac ttgatcggga aggtagtcga gcgggcgcgc cggcttggcg
     5341 tgagcagttg ctgtgtaacc ttcagccctc acccgcagga ggtgcttcgg ccggaggttg
     5401 aactctctca cctgtgcacc ttgaaggaca ggctggatct catcaagggc ctgggtgtag
     5461 acgacgttct ggtgatcgag ttcacaaggg ctctcgcgca gatgttgccc gaggagtttg
     5521 ttcacctcct gctgcgccgg cacaggatcc gggagttgtg ggtaggaagc gacttcgccc
     5581 ttgggcgaaa ccggaccgga actcccgaca agcttgccga gatagggcgg gttgaggggt
     5641 tcgaggttcg gccattcgac cgtgtggaga ggttcggggc gccggtcagc agcagccgga
     5701 tccgagggtt gcttgttgaa ggcagggtag aggaaacggc ccagttgttg gggagaccct
     5761 atcggctcag cggcgtcgtc ggacaggggg accggagggg acgaaccctg ggctttgcca
     5821 ccgcaaacct cacacagatg gacaacctgt gcgtaccagg aaacggagtt tacgcggtct
     5881 gggggcgaac ccgtgccggt ctcgtgtatc cctgcgtgac gaacgttggc atacacccta
     5941 cctttggtgg cacggcacgt caggtggagg ttcacctgct ggatttcgag ggcaacctct
     6001 atggtgatag cctgaacgtg gatttcgttg gccggttgcg ggacgaacta cggtttgact
     6061 cggttgagga gttgatcggc cagatgaagt ccgatgtggt ggatgcgcga cagcttctgg
     6121 cagggactcc gccggtagcc tgattgccaa tggcttaatc cgatccagtt cagtcattgc
     6181 ccaacccgac agcctatgct aggattatga ccacctcgca ctgatcttcg tcgcgaccat
     6241 atatccctaa aggtggtcca gagagaggcc caatggcttc gattattgcc atctctaacc
     6301 agaagggtgg tgtcggtaag accaccacca cagcgaatct cggcggtgcc ctgttctaca
     6361 tgggccgaaa ggtcttgatg gtggacatgg atcctcagtc caacctgacc tcggcctttg
     6421 gcaagagtga gcagacggag acgagtatcg cggaggcgtt gttggaccgc aaggtccctt
     6481 tgcccgtcgt tgaggtggcg gcgcaggttg acgctcggct tgacatggtc ccatctaccc
     6541 tcgcgctggc cagtgccgag gctgccctga tgaataagct ggggcgcgag ctgagacttc
     6601 gcgatcaact ggcgccgatg gggacgacct acgatttcat attgatcgat actcccccat
     6661 cccttggtct tcttacgatc aacgctcttg tggccgctga aagggtggta atccccaccg
     6721 aggccaggct cttctcgcta cagggactgc agatgatgca ggagagcatc gaggagatcc
     6781 tttacctgaa ccccaggttg caggtgcttg ggatcgttct gagcaagttg gatcgccggc
     6841 tgaaggagga gaagctggtc agcagctatc tccgagagcg atggggagcc caggtttttg
     6901 agactgagat cgggacgaac agcaagatcc tggaggccgc ctcatccggt acttccgtgt
     6961 tcaactatgc aggcgcagag aaggccgctg agagttatct ttcccttgca agggaggtgc
     7021 tgggtcgtgg ctaagatgcg cggcgggttc gatcctgatc gtttgccaga gcagacccga
     7081 agcagcccga tcatcgggaa gatagtctcc gaggagatcg agaccgaggc gaagggcttc
     7141 accgagatca agatcgtagg agtcggcggc ggcggcaaca atgcgctgaa ccggatgatc
     7201 gaggcgggca tacggggtgt cgatttcgtt gccgtcaaca cggatgccca ggcgctggag
     7261 atgtcgctgg ccacccacaa gatccccatc ggtggaaagg caatgaagcg gctcggcgcc
     7321 ggcggcaacc ctatggtggg tgagcgggct gcagagatca gccgggatca gcttgaggag
     7381 acgctggccg gtgccgatat ggtgttcatc actgcgggca tgggcggcgg taccggtacc
     7441 ggggcttccc cagtgattgc cgagatcgcc aagaagaacc gatcgctggt agtgggagtt
     7501 gttacccttc ccttcacctt cgagggtagg cagcgtagga agaccgccga acaggggttg
     7561 gaagtgttga gggatcgggt ggatgccata atcgcgattc ccaacgaccg cctcctgcag
     7621 atggccaaca agaagacagg ggtcctcgag gcgttcaagc tggccgacaa tatcctccgg
     7681 cagggtgtgc aggggatctc ggatctgatc accgtgacgg ggctgatcaa cctggatttc
     7741 gccgacgtga agacgatcat gagcggcgca ggcaccgcct tgatggctat cggcgaggcg
     7801 aagggacctg ggcgggcact cctcgctgcc aagatggcga tcgagagccc tttgctggac
     7861 gtctccatca ccggcgcgac cggcttgttg attaacgtga cgggcggccc ggacatgagc
     7921 ctgatggagg tgagcgacgc ggccgcagcc attgctgacg ccgctagccc caacgcgaac
     7981 atcatcatgg gcgcggtggt caaccccaga ccccagccgg agatccaggt tactctgatc
     8041 gccaccgggt tcggtggagc ggaaatcaag aagcccgaca tcaaggtgag cgaggagttc
     8101 gcggcgcggc tcgagcggca gatcggccaa caacagcagg cccatcagcc gcgggtggag
     8161 ccgactccca ggatggatcc ggcgcccagg atcgaggagc agtccagggg gcaacgaacc
     8221 cccgatcgcg agccgtggga gacgcctgtg ccgctcgatc gcgaaggaga tgaagactgg
     8281 acggttcgag atgtgcgtgg acccggtcgg gagtcgtggg agccgatgga tccgggtgag
     8341 actcggaact cgcgagcgcc gcgggagccg gatcgaaacg atctggacat accagctttc
     8401 ctgcgccgca aacgaccgca gcaatagaaa aaaaagaaag gggggcgacc caactgggtc
     8461 gcccccctcc tgattccagg actacttctc ctgctctttc tctttcttcg cctcggctat
     8521 cactgcctgc gccgcgtggg ccggcacctc atcgtagccc gagaacttca tggtgaactt
     8581 gccacgggcc tgggtgatgg agcgcagatc cgtcgcgtag cggcgaacct cggcgtaggg
     8641 agccctggcc tggatggtgg tgaagccatc ttccgggatc atgcccagta cctgggcacg
     8701 cttgctgttc agatcgccga tcacgtcgcc ggtataggca tccggaatcg tgatcgccat
     8761 ctccatgatc ggctccagca gcaccggctt cgccaattca gccgccttct tgaagcccat
     8821 ggaggcggcg atcttgaagg acatttccga ggagtccacc gggtggtacg aaccgtcgta
     8881 cagcgtcgcc cgaacggcta cgaccgggaa gccggccagg atgccctcct ggagagcctc
     8941 gcgcacgccc ttctctacag cgggaatgaa gttcttcggc accgcgccac caacgacgcg
     9001 atcctggaac tcgaactcct ggtccctggg cagcggctcc agttccagcc aggtgtcgcc
     9061 gaactggccg tggccaccgg tctgtttctt gtagcgtccc tgggccttgg ccgagctggt
     9121 gatggtctcg cgatagggaa ccttggggat gccgatggtc acgtcgacgc cgaatttcct
     9181 cttcatccgc tccgcggtga tgtcgatgtg ggactcgccc atgccggaca tcagcaggtc
     9241 gccggtttcg gcgttcttct ccaccctgat ggtgggatcc tcttcggtca tcctcgccat
     9301 cgccgtgccc agcttgtcca ggtcggcctt ggtctttgga gcgattgccg ccgagaagac
     9361 cggatcaggg aactcgatgc ccacaagctg gataccagca tccttcgtgg tcatcgtgtc
     9421 gcctgtggat gtctcctgca gcttggcgac tgcaccgatg tcgccaagtg cgatcttggg
     9481 caccgggtcc tgacgtttgc cgaccagcgt gtagagctgg ccgatgcgct cttcgcgtcc
     9541 tcggttcggg ttccagatgt gagagtcgga ctggaccgcg cccgagtaga cgcggaagta
     9601 gttgagtctg cccacgtagg ggtcggagac ggtcttgaag accagagccg ccagctgatt
     9661 cttccctgca agttccttgg ccgggacatc gccattggtg gttcggaccg tcgactccag
     9721 cggcgacggg acgtaggaaa ccatggcatc gagaaccggt gtgatggcac gattggccag
     9781 ggcggagccg cacatcacag ggaagaggga tcgggactgg acaccagact tcagggctcg
     9841 ctggatctcc tcgacgctga tctcctcacc ctcgaggtac ttgttgatca gttcgtcgtc
     9901 tacctcgact acggtctcga tcagcgcgtc gcgttgcgcc tgggcggcat cccgcaggtc
     9961 accagtgact tccttgggag gctccttccc ttcgccgatc acggtcatgc ttatcaggtc
    10021 aacgaccccg gagaagttct gctgcgagcc tatcgggatc tgcagcgcaa caacgccctt
    10081 gccgaacctc gtcctgaggg cctgcaggac ggtgtcgaag ttggcgttct ctcgttccat
    10141 cttgttgaca aagaacatcc tgggcacgcc gttcttctcg gcttcctgcc aggctgtctc
    10201 agtgccaacc tccagcccgg atactgcgtc gacaaggatc agcgccccgt ccacgacacg
    10261 cattgtctgg acgacttcgc caacgaaatc ggcgtatccg ggggtatcca gcacgttcaa
    10321 cttgtgaccc tgccactcac acggtgccag agcagtgctg acggagatat gcctcttgat
    10381 ctcatcggga tcgtagtctg tggtagtcga cccctcctcg acttttccca gacgggtgat
    10441 agctccactt tggaacagca tcgcctcggc cagcgatgtc ttgcccgcac cgccatgaga
    10501 gaagaggcct acgttgcgga ttttctccgc ctggtattcc ttcatccgat gctccttccg
    10561 ccatatcctg gaagggacgt cgcatctcgc ccattccatc ggtcctaaac tagaattata
    10621 ccagacaccg gtctcggatg ctcctgggga tggggggtgg gccacacgtg ggtggagcgc
    10681 ggttctggcc tcatatgggg tttgccgtgt cgctcatggg cggttcttgg tcagtctggg
    10741 cgccatatgg tagaattggg agaggctgat aggttgggga ggaggtgatg cctagtgtcg
    10801 ggacactcca aatgggcaca gatcaagagg cagaagggcg ttaacgataa caagcggggc
    10861 caggttttca cgaagctggg ccgcgagatc actgtcgctg ccagagatgg cggaggcgac
    10921 cctaatgcca acttccgcct gcggctggcc gttcagcatg ctcgtgagaa caacatgccg
    10981 atggacaaca tcgagcgggc catcaagagg ggaaccggtg gcggcgaagg tgccgctctt
    11041 gaagagatca cgtatgaagg atatggggtc ggtggcgctg cgatcctggt agaggcaatg
    11101 accgataacc gcaatcgcgc ggctgccgat gtcaggagcg ttttcactcg ccatggtgga
    11161 agcctcggcg agaccggttg cgtcaactgg ctgttcgact ccagggggat tatctcgatt
    11221 gacgtaggca agcaggatcc agatgagctt gccctgctgg caatcgacgc cggcgcggac
    11281 gatgtgaagg tcgaggacgg atccctggag gtctacactg cccccggaga tctcgagagt
    11341 gtgcggcagg cgctggaagc taacaagctg accatcacta atgccgaggt ctcgatgata
    11401 cccaagacca ctgttgagct ggatcccaag ggcacggtta cggtactgaa gatggtggag
    11461 aagctggaag acctggacga cgtccaggcg gtctactcca acatcgaggt ctccgatgag
    11521 gcccttgcgg aattctcgta aggccgggtg gcaacagata ggattgtcgt cctgggcata
    11581 gacccgggga cggcgatcct cggctatggt gtcgttgcac tggaggggga cagtctctct
    11641 gccctggact ttggggtgtt gactactccc tccagtctgc cacttacctc tcgactgctg
    11701 accctattcg acggcctcac agaggttatc tcccggaaca acccttccga ggtggccgtc
    11761 gaacagctct tctttgccag gaacgtccag tcggcgttgg cggtcggcca cgcgcggggc
    11821 gttgctctgc tctccgccgc tcgccattgt cttccagtca gcgagtatac cccgcagcag
    11881 gttaagcaag cggtggctgg ctacggccgc gccaccaaga tgcaggttca gagcatggtg
    11941 cagatcatgt tgagcctgca gtcgatcccg cagccggacg acgctgccga cgcattagcc
    12001 atcgccatct gccatgcacg ctggaacgaa ttgacgggtg gcgggagggt tgtactgtga
    12061 ttgcagggct tcacggcact gttgaggaga ttggcggcga cttcctgctg attaacgtcg
    12121 gcggcgtggt ctaccagaca tatgcctctt ctgcgaccct ctcctcggta tccagtcccg
    12181 gcgcggtgat ccatctgcac acccacctcc aggtacgcga ggacgggatg accctcttcg
    12241 gtttcgccag ccgcgaggag ttgcgcatgt ttcaactgct cctctcggtt tcaggagttg
    12301 ggcctcggat agccctggcg ctgctttcca cgatgtcggt ggaggacctg atcgcggcaa
    12361 tagccgcgga ggacactgca aggctgagca cagtgaccgg tgtggggaag cgcactgccg
    12421 cgcggatcgc actggaactg aaggcgaaag tcgggcaggc tcaggtcgcc gcgatggccg
    12481 cgtcgcctgg aaactccgca caactcctcg acacactcac cgctctcggc tacagcagca
    12541 ccgaggcggc atccgcggtt cgggccatac ctcacctcca gaccatggag ttggaggacg
    12601 cgctgcgcga ggcactgcgc tggctctcct ctgcccgata gcactcccaa cgggtggagt
    12661 tttgcccacg tccaagggta tgcatcttgc cttctggctc ccgccggagt cctagagtcc
    12721 ttgcaccttg ttacatcata gggaggtgtc cagaggtgag ggggttgatg ctggctattg
    12781 gggccgtggg gggcgtactg atcgcctttg tggcggccat ggtgaggaga ggcgggatct
    12841 ctgagatggg agcccctgtc tttggcgggt acggccagga aacgggcatg gccggactga
    12901 atcgagttcg ggctgatgtt gagacgataa agcgcgaagt agagtcgatg gagcagtccg
    12961 tcgggtcgat gcatcgggag ttgctgtctc gcatggacga catccgctcc cagttgggag
    13021 gcgctggggc tccgggtggt gcccctccgg cgtctcgtgg gactgaaaag acgactggaa
    13081 ccaggactcg tcggacaacg ggtgcggccg cgggcgggcc tcaaggggcg acaaccgctc
    13141 ccgaacgacc ggccaggagg aggacgcgga aatccgaagc cgccgtggcg gccgaggctt
    13201 tcgctcagcc ccccacttcc cggtcccaag gcaagaccca gcagacgtcg ggcggggggc
    13261 aaacgcaggg tcagtccgga cgctaggaac gcagcaaccg attccgccgc atggctgatg
    13321 gcgaacgtcg ccggccaatg gagtagagac cctatcccga gcgctgttac agcttcggcc
    13381 aaggcctcta ctccattctc tttgggttag tctgcatggt aagcttgcta cgcgccccgt
    13441 tctgagtgta gaatctttcc gctagtaagc tggaggagtc tgtgcaatgg cagaacgggg
    13501 aagacgcaag gccgaagtcg aagattacga ggtcgcggag cccgctgaag tcgaggtaga
    13561 ggaggcggta gcccctgttg ccgaggaggc ggagaaaccg gtagaggccg ccgaggaggc
    13621 tcgctacttc gtggatccgc aatggtacga ggagcgaggc ctggtgttca gcaccgtggc
    13681 tcaggctcgg ctgtgctact cctgtgcctc gaagttgggc actgaagttg aggagcgtta
    13741 tccggtcatc gacccgaaga cgaagagggt cacatttgag ttcaggcgcg ttccctacgc
    13801 agccaacccc ctacccatta tccgcgactg ttgttctcga gctcgagact acataacaaa
    13861 cgagacgccg ctgttggaag ccgttttccg ggttttcctt gccaacggga atcagcctat
    13921 gacggcctca acggtgaggg agcatctgtt gacctacctt ccggagatgg cggcactgcg
    13981 gtccgactat ccggtggagt tgctggagcg gttgattagg ggcgacaaga actatggatt
    14041 gagggagcac acggttcccg tctcggcttg atggtcttgg atggaagtag ggcggagcct
    14101 tgtgccccgc cctacgtttt gtccttggtg gttctagata tgcacggttg aggcgcgcta
    14161 tctagctgtg agccaattgc cacctaatcc tctgtgctac agagccagcc ccttcaggta
    14221 ggcacggaag tcatcgctca ggtccggcct cttcagcgcg aaatccacgg tggcctgcag
    14281 gaactccagt ttgttaccgg catcgtagcg cttgccgtca aattccagcg cgaacacctt
    14341 ctgacgccgc atgagcgact tgatggcgtc cgaaagccag atctcaccgc ccttgccggc
    14401 aggggttctt tccaactcct cgaaaatctc aggtgtgagg acgaagccct tgacggccgc
    14461 cagatcggag ggtgcactgt cctgactcgg cttctcaacg aggtcgatca cttcgtggat
    14521 ccgctcggat accgggcgag ggtctatgac accgtatttc gatatgttct ccttggcaac
    14581 cctcatcacg gccatgacgg aagtgccgta ctgctcgtat acgctgatca actgctgaag
    14641 gcagggaacc tgcgcatcga ccaagtcgtc gccccacagt accgcgaatg gctcgtcacc
    14701 gattacctct cgtgccacca gaacggcgtg tccgttacct agaggctgct tctgtctcac
    14761 gtagacaaag ttggccaact ccgagagacg ctccacctcg tggagttcat cgatcttatt
    14821 gttctcgcgc aggcgatgag caagctcgaa tgggtagtcg aagtgatctt cgatggctcg
    14881 cttgctctgc cctgtgatga tgacgatttg ctctatccct gaggcgaccg cttcctcgac
    14941 gatgtactgg atgaccggct tgtcgacgat gggaagcatc tccttgggct gggcctttgt
    15001 ggccggtagg aagcgggttc ccaacccggc cgcggggatg accgcttttc tgaccttctt
    15061 caagtcttgg cctcctaaac tcgtagaatg gtagggcggg cacgctgccg cggtccgctc
    15121 atctgttgtc gttactcaac gccggatggg gctcttccat ctcgggctgt tcatctgagg
    15181 gtgtagcttg ccagctaccc tcatcgacct tctcctccat atgcactttg cgctcttcca
    15241 ccgtcattcg gtagaaggag accatggcta cgataacacc agccacgggc accgcaaaga
    15301 gggcacccca catgcctgcc aatttcgacc cgaccaacag agacacgagc acaagcagcg
    15361 gatgcattcc cacgcttctg ctcacgaacc ttggaccgag gacgttcagg atcacctgct
    15421 gcatcagaag caggattatg aagacccacc aggctcgacc cgggtgaatt agaagggcga
    15481 tgaccacggg ggggatcagg gccagcatag gtccgatcac cggaacaagc ataattgcca
    15541 tgccgaagac ggatgccgcc gccgtgtagc tcaggtcggc tgccatcata acgccgaccg
    15601 tgaccaggcc gtagatgaga gcctggatca actgtcctcg aatgaagccg ccgaaggctt
    15661 gatgggtgct gtagatcagg taatttacgt catctcttct atcgatcggt atagccttga
    15721 gcactgccgt cgcgaaacgg gatccgtcta gcgccacgta gaaggagaac atcagcacca
    15781 gtatcaggtc gaacaggagg gaggcgacac cagtggcgag ggccagcgca ttgttgacga
    15841 ttggcggccc catagactcc acgtagcggg caatctgatt gtagtccagt atcctggttg
    15901 cgttcacgtc caggccgcgt gcggctgcct gatactgcag gcttgtcagc cagtcgcctg
    15961 cggcagccac gtaaatcggc aggttaatgc cgatctgggt cacttgcagt gcgatcactg
    16021 gtatcaggag gatggtgaat aggctgagta caaccaggag ccccaggtag acgatgccga
    16081 cggcgacggc acggtttaac ctgtagcgcg tctccagggc gtaagcgacg ggctccagta
    16141 caaaggcaag cacccacgcc agaacgagca gcaacaggat atccgcgaaa cggactgcca
    16201 gtgtccatac caggcctgcc agatagctcg cggcgatgag gacgagaagg atgatcagcg
    16261 tctttagcca gggatctcgc tgcaagtaga tacggctccg gttacattat ggttacgcgc
    16321 agggattcta gcactctgtg tgagagtttt gcgagaaggc cttgttataa agataccatg
    16381 attaggtatc gtattctggt tgcagacccg aaccctgccg ttcgtaagtt gctgtcgatg
    16441 gtgttcgact ccgagcggtg cgagctcagc ttcgcatccg atggcgagga ggcgctgggg
    16501 atggccattg tagccccccc cgatctcgtc attgccgagt tgcgcctcga ccggctggat
    16561 gggattgccc tgtgtgagcg gcttaaagag cattctcgta cagtgaagac aaagctgctg
    16621 ttccttacga cctgcgcttc cgaatgggag atgcgccgtg caaggagagc gaaggcagat
    16681 ggctacatga ccaagccatt cagccctatg gcgctgcttc gtcaggcaga cgagttgttg
    16741 gaaggcaagc ccgtcgacaa gggcgaggat ctgtcgtgtc gattgttgga caaaggtagc
    16801 tgaagccagc cgtcacagtt ttgatgccag cgtgaccgcc tcgatgttct caattttgaa
    16861 catcattacc ctcttcttct gatggcatcg ccccaccaga taccaggtgc cgaggtaagg
    16921 catgaggagg ctgggctcca ccacgggatc gggtatgccg ccggctgcgc gggcagagag
    16981 atgcagacga actggcctgc gctcgaacaa cgcttctgtc agcaccagga atagcttgtc
    17041 ctctagagcc ggctctggat tacccaggga ctccagactc ttgcgaatga gtggctgaag
    17101 gtgaagcggg aagaccgcgg gcaatttcgc caagagggaa cggagcggct cctggggcac
    17161 ggtccggtcc gtggtggcaa tcttcagcgc tgatatcagc gcccttgcct cggagaagga
    17221 aaagtccagc acaacgtggt cgtcgccggc tgagaaccgg taccctttat ggcgtaccag
    17281 agggaaatga agctcctctc ggattacccg caggtcctct tgaacctgcc gttcgctgag
    17341 ggagtaccgc tttgctagct tctggcggct ggttccgggg tgctcggcta tgtattcgac
    17401 gatggcccag atacgtccaa gccgatcgat ggccagctgg tcccgttcca ccgtgtcggt
    17461 cacctctgaa ggatgaatgc tgggctatgc gacgcggcca tccgggtgaa gacaaaacaa
    17521 aaccgcggtc aaccgatgat agagaaccag ggccggtcga ccagcggctg gcataagccc
    17581 gggctaacgt caccgatggg tggccgtcct gagcaagctg acagctcccc tgagatctgt
    17641 ctgccgtcac cactgtaatc tggcggcaga ccagcccgct gtgtgagaaa tgagggtacc
    17701 ccggatagga ctcgaaccta cgacctttgg gtccgcaacc caacgctcta atccactgag
    17761 ctaccggggc aagaaggcgg aggagatggg attcgaaccc atgacggagg tttaaggccc
    17821 ccgtaaccgc ttagcaggcg gccgcactag accaactatg cgactcctcc tgagcctgtg
    17881 cgagccacct ggaaacggtc tccagagagg gttcggcgga gggggaggga ttcgaacccc
    17941 cggggctctc gcccgaccgt tttcaagacg gccaccataa accgctcgga cacccctcca
    18001 ggtgcgcgcg actatttcaa tgttaaccga ccagccgggg agcaacccca ggactgtccc
    18061 ttgattctat ccgacccg
//
LOCUS       DATALS010000530         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_126137, whole genome shotgun sequence.
ACCESSION   DATALS010000530 DATALS010000000
VERSION     DATALS010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126137"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VHS06_11865"
     CDS             <1..472
                     /locus_tag="VHS06_11865"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX2988849.1"
                     /translation="QPRLSLVNSVTLLLAATVLVAVESELLVGGISDATAALGMTEFF
                     VGIVVVAVVGNAAEYFTAMMMAKRNRMELSVTIATGAGTQVALFVAPVLVFASFIMGQ
                     PMSLVFNPFEIAGVTLTVLALALVALDGESNWFEGLQLTAVYLILAIVFFFVPA"
     gene            complement(500..685)
                     /locus_tag="VHS06_11870"
     CDS             complement(500..685)
                     /locus_tag="VHS06_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988850.1"
                     /translation="MVPFALRRGNSDSGDIATARSVAANLSVLIGGLFLRWMTVQAGK
                     ESADSPTTYFDFTRDRE"
     gene            complement(642..1538)
                     /gene="nrfD"
                     /locus_tag="VHS06_11875"
     CDS             complement(642..1538)
                     /gene="nrfD"
                     /locus_tag="VHS06_11875"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX2988851.1"
                     /translation="MIEELRRLDTDSITDIMRRGKAPPSRAPKPEHPSYYGYPAIKRP
                     EWRSEIPVYFFAGGLAAGSYLVATLADLFGTEEDRVVARIGRYLALVTTLLCPLLLIA
                     DLGRPERFTHMMRILKTRSPMSMGSWGLMGFGLFVGLGALRQAVEDGLIGARTVMARL
                     LAWVPFRASGILGSLFAFFISGYTGVLLTFTNVPLWASNRLLQAPLFITSALSSGVSA
                     ISLILTARGGAPKATRAWLDRTGNAAALGELAMTVGSMGTLRSQAWPLLTVRFGLPYW
                     LGTVGMGANGPLRPTKGQQRFR"
     gene            complement(1584..>1791)
                     /locus_tag="VHS06_11880"
     CDS             complement(1584..>1791)
                     /locus_tag="VHS06_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2988852.1"
                     /translation="STGGVGALHCFFLLTERPEYYNLPPAPELPSTRVLPASIAAAAT
                     ALVYGAIAVAAIQGAARRGFRLRG"
BASE COUNT          317 a          551 c          568 g          355 t
ORIGIN      
        1 ccagccgagg ctgagcctgg tcaactcggt gacgctgttg ctggcggcta ccgtattggt
       61 ggccgtggaa tccgaactgt tggttggcgg catttccgat gccactgctg cgctgggcat
      121 gactgagttc ttcgtcggca tcgtcgtggt ggcagttgtt gggaacgcgg cggagtactt
      181 cacggcaatg atgatggcca agcgcaacag gatggaattg tccgttacca tagccaccgg
      241 cgccggtacc caggttgcgc ttttcgtcgc gccggtgttg gtgttcgcgt ccttcatcat
      301 gggacaaccg atgagtctgg tgttcaaccc gttcgagata gccggtgtta cgctgaccgt
      361 cctcgctctg gcactggttg cactggacgg cgaatccaac tggttcgagg ggttgcagct
      421 caccgcggtc tacctgatcc tggcgatcgt cttcttcttc gtccctgcct gatgtcggtt
      481 gaaggtgtga gtgtgcgtct tactccctgt ctcgtgtgaa gtcgaagtag gttgtcgggc
      541 tgtccgccga ctccttccct gcctggacgg tcatccatcg taggaagagg ccgccgatca
      601 gcacagacag gttcgcggcc acgcttctcg ccgtggcgat gtcaccggaa tcgctgttgc
      661 cccttcgtag ggcgaagggg accatttgcc cccattccca ccgtacccag ccaataggga
      721 aggccgaatc ggacggtcag caagggccag gcctgggagc ggagagttcc catgctcccc
      781 accgtcatcg ccagttcccc aagcgccgcc gcgttgccgg ttctatcgag ccatgctcga
      841 gtggctttcg gcgcgccccc acgggccgtg aggatcagag agatggcgga gaccccgctg
      901 gagagcgccg aggtgatgaa gagtggggcc tgcagaagtc ggttgctcgc ccacagaggc
      961 acgttggtga aggtcagcaa gacccccgtg tagccgctga tgaagaaggc gaagagactg
     1021 ccaagaatgc cggatgctcg gaagggtacc catgccagga ggcgagccat cacggtgcgc
     1081 gcccctatca gcccgtcctc gacggcctgt cgcaacgctc ccagcccgac gaatagcccg
     1141 aaccccatca acccccagct acccatggac atcggtgagc gggtcttgag gatgcgcatc
     1201 atgtgggtaa agcgttctgg gcgccccagg tcggctatca gcaggagcgg gcagagcagc
     1261 gtcgtgacca gcgcaaggta gcgaccaatt cgggcgacca cgcggtcctc ctcagttccg
     1321 aaaaggtcgg ccagtgtcgc cacgaggtag gagccggctg caagcccacc cgcgaagaag
     1381 tagaccggga tctccgagcg ccactctggg cgctttatgg ctggatagcc gtagtagctg
     1441 gggtgttccg gctttggagc cctcgagggg ggcgccttac cacgacgcat gatgtcggtg
     1501 atggagtcgg tatccagtct acgcaactct tctatcacgg ttgctgttcc tcacactctt
     1561 ctacgatctc aaattggcga ggcctatcca cgaagcctga agcctctcct ggccgcaccc
     1621 tgtatcgccg ctacggctat cgcgccgtac accagtgccg ttgctgccgc ggctatcgat
     1681 gccggcagca ctctcgtcga cggcaactcc ggcgccggcg gcaggttgta gtactcgggg
     1741 cgttccgtca gcaggaagaa gcagtggagc gcccccacac ctcccgtcga g
//
LOCUS       DATALS010000531         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_79160,
            whole genome shotgun sequence.
ACCESSION   DATALS010000531 DATALS010000000
VERSION     DATALS010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79160"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VHS06_11885"
     CDS             complement(<1..1541)
                     /locus_tag="VHS06_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988853.1"
                     /translation="MSFLLPYPLHHGTPPDGSGSLPDYQYGGHFFYYGSLMVVLFAAA
                     LADMIARWTRLTPRRRGTHVWTFCAVVAFGLCLGTQGYLWRLVEWLPMGLRNYPIRVL
                     PVFLLFSLVSGGLVLDRWLRQFAQRRSVGLSLFVGVCLILFYHVSCARCSFYNYGFQP
                     YPTLPADLLQLVGSPDGKPPYRNLFCAPHRSFDPTYAYCLPQALSVQYGLPALTGYDP
                     LLERKPDFMQVAERLDGDRDNTLRAYGVRWVLIHRTVWQGNPLSPGTSRILERRVPFD
                     ASHADFTTIHEPESLRGILKVGETRAPDPLCFVADSLTQALPLRFDGRGIYATLERPA
                     RGQTVVVNFLLHPAMRAYVDGKPTPLGKDDWLRIVVDVPQGAEKLEILYQPPWRQGLL
                     AGVVLAGLGLVTLPIVARVGSVSPLAAGRTLAARYPRTRPMRILPAICGAEWKRRLLL
                     DDLMPPLIASLVVYGTFAAVQSVFFHFLMTGEPPRICFTPLGLEVACNRLPSLAATLA
                     AALAT"
BASE COUNT          355 a          471 c          508 g          235 t
ORIGIN      
        1 cttgtggcga gagcggctgc cagcgtggcc gccagggaag gcagccggtt gcaggcgact
       61 tccaggccca gcggcgtgaa gcagatcctt ggcggttctc cggtcatgag gaagtggaag
      121 aagacgctct gaacggccgc gaaagtgcca tacacaacca acgaggcgat caagggcggc
      181 atgagatcgt ccaggagcaa acgccgtttc cactctgcgc cacaaatcgc ggggaggata
      241 cgcatcggcc gtgtcctggg atatcttgcg gcgagtgttc tgcccgctgc cagaggggag
      301 acgctgccaa cgcgtgccac gatcggaagc gtgaccagcc ccaagcctgc cagcacaacc
      361 ccggccagca ggccctgcct ccagggcggc tggtagagga tttccagctt ctcggcccct
      421 tggggaacat cgaccacgat ccggagccaa tcgtctttgc caagtggcgt cggtttccca
      481 tccacgtaag ccctcatggc cgggtgaagg aggaagttga cgacgacggt ctgcccacga
      541 gcgggccgtt ccaaggtggc gtagattccc cggccatcaa accgcagggg caacgcctgg
      601 gtcagcgaat cggccacaaa gcagagcgga tccggagccc gggtctctcc aaccttcagg
      661 atgccccgaa gcgactccgg ctcgtggatc gtggtgaagt ctgcgtggct ggcgtcgaac
      721 ggcacccggc gttccaggat gcggctggtg ccgggagaga gtgggtttcc ctgccaaaca
      781 gtgcgatgga tcagcaccca ccgaacgcca taggcccgca gcgtgttgtc gcgatcaccg
      841 tcaagtcgct cggcgacctg catgaagtca ggctttcgtt ccagcaacgg gtcatagccg
      901 gtcaacgctg gaagtccgta ctgcaccgac aacgcttgag ggagacagta ggcatatgtc
      961 ggatcgaatg aacgatgggg cgcacaaaac aggttgcggt aggggggctt cccgtcgggc
     1021 gagccgacca actgcaacag atcggcgggg agcgtggggt agggctgaaa cccgtaattg
     1081 tagaacgagc atcgagcaca cgagacgtga tagaaaagga tcaagcaaac tccaacaaac
     1141 agcgagagtc ccacgctacg ccgctgagcg aattgccgta accaccgatc gagcaccagt
     1201 cctccactta ccagggagaa gagcaggaac acggggagca cccggatcgg atagttgcgc
     1261 agtcccatgg gaagccactc gacgagtcgc cataggtacc cttgagtgcc caagcacagg
     1321 ccgaaggcga ctacggcgca gaacgtccac acatgggtcc cgcggcgtcg cggagtcaac
     1381 cgcgtccagc gggcgatcat gtcggccaga gcagccgcga agagcacgac catcaacgag
     1441 ccgtaataga agaagtgccc tccatattga tagtccggca ggctccccga tccgtcgggg
     1501 ggcgtaccgt ggtggagcgg atacgggagc aggaatgaca acaggtcccc cccaataccg
     1561 gatccatag
//
LOCUS       DATALS010000532         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_79192,
            whole genome shotgun sequence.
ACCESSION   DATALS010000532 DATALS010000000
VERSION     DATALS010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79192"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VHS06_11890"
     CDS             <1..881
                     /locus_tag="VHS06_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose dehydrogenase"
                     /protein_id="HEX2988854.1"
                     /translation="PDNEEWARRHPHLHDIPGRDVTLAGRNFETPDFLYGRDGAQTGP
                     FKQFGTQCTDGEVVKGATPATAAIARADSEDGGGFEVIAWGLRDVTCMAAHQDGRIYC
                     ADIGMAEWGSRPVADNHERIWQVLEAGWYGWPDFAGGVPVTDARFKPRNDPQPEFLLR
                     DHPMDNLVRDPVATLPPGSGLSKMEFSRSRMVADENTAFLALGGDFMGISDHGFRVMT
                     LDVNSGELNDFAVNKEPGPSSVTRSGGFEHPVDVRFDRTGEVMHIVDLGIVEMTPQGP
                     AVVPGTGVLWRISWTA"
     gene            complement(975..1337)
                     /locus_tag="VHS06_11895"
     CDS             complement(975..1337)
                     /locus_tag="VHS06_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988855.1"
                     /translation="MAEEQTYLLDANAPAQQMLHDIDEARAADEPDLRREHIQLALDA
                     AQSYADDLDSALTTTEDPDLSDRIEEAVHHLDMAIEQGEAAIDAPDEDLEEYLSTMDR
                     HAEQSYARLQEAVGAAEQ"
     gene            1660..2007
                     /locus_tag="VHS06_11900"
     CDS             1660..2007
                     /locus_tag="VHS06_11900"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2988856.1"
                     /translation="MEVALSVQTRIVDGKPVIDVDGEMDHTGIRQFRDEVASLIDQGH
                     RSIVLDMCHVGFMDSGGLSAIIYAMKRLESLEGCITLAGCNDLIVRKLTIGGLTKISD
                     ALRLAQSVEHAVS"
     gene            2122..>2313
                     /locus_tag="VHS06_11905"
     CDS             2122..>2313
                     /locus_tag="VHS06_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988857.1"
                     /translation="MDSRQEQKATTGQQNSKSALEAMGHLHRLSSLIASDHLDDALTT
                     AVEIPVRCLGIGGTALLLPV"
BASE COUNT          442 a          664 c          777 g          430 t
ORIGIN      
        1 gcccggacaa cgaggagtgg gcgcgcaggc atccgcacct gcacgatatc cccggccgcg
       61 atgtgacgct ggcggggagg aacttcgaga cgcccgactt cctctacggc agggacgggg
      121 cgcagacggg gccgttcaag cagttcggga cgcagtgtac cgacggcgag gtggtcaagg
      181 gggcgacccc ggcgacggcg gcgatcgcgc gggcggattc cgaggacggc ggcgggttcg
      241 aggtgatcgc gtggggcctg cgcgacgtca cctgcatggc ggcgcatcag gacgggcgga
      301 tctactgcgc ggatatcggg atggccgagt ggggaagcag gccggtggcg gacaaccacg
      361 agcgcatctg gcaggtgctg gaggcgggat ggtacggctg gccggacttc gcgggcggcg
      421 tgccggtgac ggatgcgcgg ttcaagccga ggaacgatcc gcagccggag ttcctgctca
      481 gggaccatcc gatggacaac cttgtgcgcg atccggtggc gactctgccc ccgggatcgg
      541 ggctctcgaa gatggagttc tcgcgcagcc ggatggtggc ggacgagaac accgcgttcc
      601 tggcgctcgg cggggatttc atggggatca gcgatcacgg gttcagggtg atgacgctcg
      661 acgtgaacag cggggaactg aacgacttcg cggtgaacaa ggagccgggc ccgtcgtcgg
      721 tcacccgcag cggagggttc gagcatccgg tggatgtgcg cttcgaccgc acgggcgagg
      781 taatgcacat cgtcgacctg gggatcgtgg agatgacgcc ccagggcccc gccgtcgtgc
      841 cggggacggg cgtgctctgg cggatcagtt ggacggcgta ggcagaggca ggtggcaggt
      901 tatgggtttt gggttatggg ttctgggtta tgggttatgg ggttccccgt aacccaaaac
      961 ctacaacccc taacctactg ctccgccgct cccaccgctt cctgcaggcg ggcgtaggac
     1021 tgctcggcgt ggcggtccat ggttgacaga tactcctcga ggtcttcgtc cggggcatcg
     1081 atcgccgcct ccccctgctc tatcgccatg tcgaggtggt gcacggcctc ctctatccgg
     1141 tcggacaggt cggggtcttc ggtggtagtg agcgcagagt cgaggtcatc ggcgtagctc
     1201 tgcgcggcat cgagcgccag ttggatatgc tcccgcctca ggtcgggctc atccgccgcc
     1261 cgtgcctcgt caatgtcgtg cagcatctgc tgggccggag cattggcatc cagcaggtaa
     1321 gtctgttcct ccgccatgtg caacctccga ttcagttgtc agttgctagt tgttagttgt
     1381 tagtgaaacc caagactgac aactaacgac taacgactga cgacttccgc gccgttgata
     1441 cccggcattc aaaaagctgt aaactcaggg ccacgaaaat gcctcggaat gcttgacaac
     1501 cacgcggtcg tgtgagagaa tcgcattggt atgcacgctt cgtagcagcc cgacctcata
     1561 ctaccattac agcccccagc accacgaacc gattgcatgc cgttcgctct tgcagagcag
     1621 ttcccacagc cgcagtgggt agatagtgga gcatcctaca tggaagttgc tttgtcagtg
     1681 cagacacgca tcgtcgacgg aaagcctgtc atagacgtcg acggtgagat ggaccacacg
     1741 ggcatccgtc aattcaggga cgaggtagcg agccttatcg accagggaca caggagcatc
     1801 gttctcgata tgtgccacgt cggtttcatg gacagcggcg ggctctcggc aatcatctat
     1861 gctatgaagc gtctggaatc cctggaaggc tgcattactc tggcaggctg caacgatctg
     1921 atcgtccgca agctgacgat cggcgggctg acgaagatat ccgacgccct gaggcttgcc
     1981 cagtccgttg aacacgcggt gagttgagag ggacgggtga acggtgaata gtgaacggtg
     2041 aacagtaaga ccccggactg atcactggtt accgatcact gattactgat caccgatcac
     2101 agcttttcga ggcatggttg catggacagc cgacaggaac agaaggcaac cactggacag
     2161 cagaacagca agagcgcttt ggaagcgatg gggcacctcc accgcctgtc gtcgctcatc
     2221 gcctccgatc acctcgacga cgcgctgact accgcggtcg agataccggt gcggtgtctc
     2281 ggcatcggcg gcacggcact gcttctgccg gtt
//
LOCUS       DATALS010000533         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_127454, whole genome shotgun sequence.
ACCESSION   DATALS010000533 DATALS010000000
VERSION     DATALS010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127454"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VHS06_11910"
     CDS             <1..313
                     /locus_tag="VHS06_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988858.1"
                     /translation="RLVDEAVTEAKDELVVQVSEMLPALVGFISKKVQHLIETDAEVT
                     PRDLQALSTTAGIWADKMALWDKARSTDGGGEERPPLPPSDVLKLVGGKTSGKSEKPA
                     S"
     gene            310..549
                     /locus_tag="VHS06_11915"
     CDS             310..549
                     /locus_tag="VHS06_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988859.1"
                     /translation="MKHPTPDLAELMKSEEFQQRYAKIKEQMEAMQAVKDEEKGIVLM
                     FKALDALKRGDRPRWRRRMKRACELCRIDPAKYGV"
     gene            1156..1452
                     /locus_tag="VHS06_11920"
     CDS             1156..1452
                     /locus_tag="VHS06_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988860.1"
                     /translation="MRMQWFTRLTLRRRLARALGLPVIGSDVPQRTKARAFLKSKDGK
                     RVHLSDFEKRFRITAVNDDRKIDGYSKSPYYQPEDPNRIKRNRKLTDKFLASKS"
     gene            1595..2263
                     /locus_tag="VHS06_11925"
     CDS             1595..2263
                     /locus_tag="VHS06_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988861.1"
                     /translation="MLKDSLLRDFEKSVHRWYPFARDSILFYETEEGQRATEAITELR
                     DVLDDFYFAGKAESEQEARELLGCAVDHLRSAAVEPLERATENRLYRVEAARQQLKGC
                     KAFLWHILFMHIPSASQVQPMIDLTKEHIREGRRLKSKLETAEQAIQHFQAAHKQLVK
                     LETLYEGTDGGNRLFTLISGFLLLFVFPIGGFIWGLIGWTPNLRPVVESVLRTLLVWV
                     AAPQ"
     gene            complement(2300..2455)
                     /locus_tag="VHS06_11930"
     CDS             complement(2300..2455)
                     /locus_tag="VHS06_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988862.1"
                     /translation="MKGSTKPFKRLTPEQMDAVVPELIRLGIIQAGETEEVDLSERAR
                     SSNGEGD"
     gene            complement(2669..2896)
                     /locus_tag="VHS06_11935"
     CDS             complement(2669..2896)
                     /locus_tag="VHS06_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988863.1"
                     /translation="MLAEGSQPKKRGPLGRWVMLAIHGLVVILAVLCVLSVLHGATGA
                     LISGFLLPAPVAPGDRLAKGWLYWLEFWRKD"
     gene            complement(2905..3261)
                     /locus_tag="VHS06_11940"
     CDS             complement(2905..3261)
                     /locus_tag="VHS06_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988864.1"
                     /translation="MTLARYSQQYAPGRTNAGDYVEPIAVADIINRRAAGGWTLMEKQ
                     STDTKAATVLVFSRRVPWYESSRDLPPTPAPLPTPVPAATKPPAAPAPTMAPAARPPT
                     MAPAPVATMAPAASSR"
BASE COUNT          723 a          943 c         1059 g          663 t
ORIGIN      
        1 ccgcctggtg gacgaggccg tgaccgaggc caaggacgag ttggtggtgc aggtctcgga
       61 gatgctgccc gccctggtgg gcttcattag caagaaggtg cagcacctga tcgagacgga
      121 tgccgaggta acacctcgag atctgcaagc cctctccacc acggctggta tctgggccga
      181 caagatggcg ctgtgggaca aggcccggag caccgatggc gggggcgagg agcggcctcc
      241 actcccgcca tcggatgtgc tcaagttggt tggcggcaag accagcggga agagtgagaa
      301 gccagcgtca tgaagcaccc aacccccgac cttgccgaac tgatgaagag tgaggagttc
      361 cagcaacggt acgccaagat caaggagcag atggaggcca tgcaggcggt gaaggacgaa
      421 gagaagggca tcgtcctgat gttcaaggcc ctggatgcat tgaagagggg cgaccggcca
      481 cggtggcgcc gccgcatgaa gcgtgcgtgc gaactctgcc ggatcgaccc tgccaagtat
      541 ggggtgtgac cgcgcagcta acgggcggtc tcaacttccc ggcagagatg gcagacacac
      601 aatggaggga caatggcaga ggcaacacag caagcggagt ttgagcgcga ccgggagcgc
      661 ggcacggcag gaactggccg ctccagagga acttcaagct cgtcagaaga ccgaaacctc
      721 tgctgccggg cataccaccg taggcgagtg ccccatggga tagccacgtt agaatcactc
      781 cgattgggtg attcccaccc tccgattgtt gaacgagaac ttagaggctt gagttatcat
      841 cttgaagggg gtagactgca tcatcctcaa gattcggctt ggccacgccg atatcatacg
      901 tagacgagat caggtcctcg cattggtatg gctgatgcac ataggtattg atgcgggcag
      961 atcgtggcga catgcgtcat gttccgcacg tcagcttgac acccggacag gcaactgcta
     1021 ttactatgta gcataactcg tctagatgcc tccggtggca cttatgctgt agggtgtcgc
     1081 cgtatggtcg gggttctgcc cggcgatgga agtgtaggcg gggcagttca gaccgttctc
     1141 caaggaggtg gtgatatgag aatgcagtgg ttcactcgtt tgacccttag gaggcggttg
     1201 gctcgggcac tagggttgcc ggtgattggc agcgatgtgc cgcaaaggac caaagcgcgg
     1261 gcattcctga agtccaagga tgggaagcga gttcacctta gcgattttga gaaacgcttc
     1321 aggatcacgg ctgtcaacga tgacaggaag atcgatgggt actcgaagtc cccttactat
     1381 cagccggagg acccaaatcg catcaagcgg aaccgtaaac taacggacaa gttcctcgcc
     1441 agcaagtcat aggcggcgac cacgctgtca tccgcggact tggccctgag ccactgaaga
     1501 acctggtcta cccgaaggta atcgaccagg ttctttttgg ggctttgcgc tcacttcggg
     1561 tagaacgaca agactcgccg acggaggggt tctcttgctc aaggattccc tgcttcgcga
     1621 cttcgagaag agcgttcacc gatggtaccc gttcgcccgc gactcgatcc ttttctacga
     1681 gacggaagag ggtcaacggg ccacggaggc tatcacggag ttgcgggatg tcctcgacga
     1741 cttctacttc gcagggaagg ctgagagtga gcaagaagcc cgcgaactcc ttggttgtgc
     1801 cgtcgatcat ctgagatcgg cagccgttga accgcttgag cgggcgaccg agaatcgcct
     1861 ctacagagtg gaagcggcac gacagcagct taaggggtgc aaggctttcc tctggcatat
     1921 cctgttcatg cacattccat cagcgagcca agtccagccg atgatcgact tgacgaagga
     1981 gcatataagg gaagggcgta ggctcaagtc gaagttagag actgctgaac aggcgataca
     2041 gcattttcag gctgcccaca aacaactggt caagctcgaa acgctctacg aaggcacgga
     2101 cggcggcaac cggttgttca cgctcatctc tggctttctc ctattgtttg tcttcccgat
     2161 aggcggtttc atttgggggc tcattgggtg gactcccaat ctccggcctg tagtggaatc
     2221 agtcctgcga acgctacttg tgtgggtggc agccccgcag tgacaacgca atgggcgaca
     2281 agccatattt cctagccttc tagtcacctt cgccattaga tgacctggcg cgttcggaca
     2341 ggtccacctc ttctgtctct cccgcttgga tgatccccag tcgtatcaac tccggtacta
     2401 ctgcgtccat ctgctccggc gtaagcctct tgaatggctt cgtcgatccc ttcatgtgtt
     2461 cttccggagg tgttggcatc gtcttgctcc ccctccctgc gcatggcgtc tcgatcctca
     2521 tcgacgagtc tgcatgacga accagccgag ggcagcggcc gtgttgcagg gacgcgacat
     2581 gggcgactta ctgaacgagt gggtgcggga tctctgcgac tggtagcagg aagagcggca
     2641 ggccacacgg cggaaggagc agtgtggcct agtccttccg ccagaactcc agccagtaga
     2701 gccaaccttt ggccaagcga tcaccgggcg ccacgggtgc cgggagcaag aagccactta
     2761 tcaatgcacc ggtggcgccg tgaaggacgg acagcacgca gaggactgcg aggatcacca
     2821 ccaagccgtg aatagccagc ataacccacc gaccgagagg accgcgcttc ttgggctgtg
     2881 aaccttccgc tagcatcgtt cctcctatcg actagacgcc gccggcgcca tggttgccac
     2941 cggagccgga gccatcgttg gtggacgcgc tgccggcgcc atggttggtg cgggcgcagc
     3001 aggtggcttg gtggcagccg ggaccggggt gggcaatggc gcaggtgtgg gcggaagatc
     3061 acgcgatgat tcgtaccacg gcacccgtct ggagaacacc aggactgtag cagccttggt
     3121 atctgtactt tgcttctcca taagggtcca gcccccagcc gctcggcggt tgatgatgtc
     3181 ggccacggcg atcggctcta cgtagtcccc cgcgttcgtc ctacctggcg catactgctg
     3241 ggagtacctg gcgagagtca tgaagtcgac cacctcagcc acagaagagg tggacctgtc
     3301 cgaacgcgcc aggtcatcta atggcgaagg tgactagaag gctaggaaat atggcttgtc
     3361 gcccattgcg ttgtcactgc ggggctgc
//
LOCUS       DATALS010000534         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_79759,
            whole genome shotgun sequence.
ACCESSION   DATALS010000534 DATALS010000000
VERSION     DATALS010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79759"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VHS06_11945"
     CDS             complement(<1..509)
                     /locus_tag="VHS06_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX2988865.1"
                     /translation="MIVGVPKEIKDNEYRVAATPGGVELLVSSGHRVLVENGAGVGSG
                     FSDEEYRRAGATMVNHREAFTEADMVMKVKEPLPSEYGLLKDGLLLFTYLHLAAEPEL
                     TKVMLEKRITGVAYETVEMPDRSLPLLIPMSEVAGRMSIQVAAYCLEESHGGRGKLLG
                     GVPGVRPSDV"
     gene            complement(713..>1691)
                     /gene="pfkA"
                     /locus_tag="VHS06_11950"
     CDS             complement(713..>1691)
                     /gene="pfkA"
                     /locus_tag="VHS06_11950"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX2988866.1"
                     /translation="GSMKRIAVLTSGGDAPGMNAAIRSVVRGGLERGMEVLGVRHGFA
                     GLIAGEMSPLTARDVGGIIQKGGTVLGSARSPLFRTQEGQRQAMVELAKRGVEALVVI
                     GGNGSQTGAYALSQMGFPVVGIASTIDNDLLGSDITIGVDTALNTALEAVDRLKDTAS
                     SHHRAFVVEVMGRDSGYLALMTGIAGGAEVVLIPEVDTDPDQVAAELRSAYARGKPHA
                     IVVVAEGAKLNAEALLNHFNTYHDQLGFELRVTILGHVQRGGSPLAFDRLLASRLGAG
                     AVEMLDKGTTGVLVGMINEKIHATPLEEVAGKRKPVDLDLYRLANVLAR"
BASE COUNT          291 a          596 c          481 g          323 t
ORIGIN      
        1 acatctgagg gacgaacgcc gggcactcct cccagcagct tgccgcgacc gccgtgggat
       61 tcctccaggc agtaggccgc aacctggatc gacatccgcc ccgctacctc gctcatgggg
      121 atcagcagcg ggagcgacct atccggcatt tcgacggttt cgtaggccac gccggtgatc
      181 cgcttctcca gcatgacctt cgtcagttcg ggctcggcag ccagatgcag ataggtgaac
      241 agcagcagac cgtccttcag cagcccgtac tccgagggga gcggctcctt caccttcatc
      301 accatgtcgg cttcggtgaa tgcctcccgg tggttcacca tggtcgcccc ggcccgccgg
      361 tactcctcgt cggagaagcc gctgcccaca ccggcgccgt tctccaccag taccctgtgt
      421 ccgctgctta ccagcagctc gactccaccc ggggtagcgg ccaccctgta ctcgttgtcc
      481 tttatctcct tgggcacacc gacgatcatc gtcgatcctc cacatgcagg ccaatgctag
      541 ctgccggcct gccagcaaca acgaagtatc tccctgccct gattgtatgc tcaaggtgtg
      601 cctcggacaa gggcgacttg cagccacaaa gaagagaatg gggggctctg cttcgactca
      661 gatccttacc gcccgtgcct gcccagcacc cagcactcag cactcagcac cgttatcttg
      721 ccaggacgtt tgccaatcgg tacaggtcca ggtccaccgg tttgcgcttg cctgccacct
      781 cttccagggg ggtggcatgg atcttttcgt tgatcatccc cacgagcacg ccggtcgtcc
      841 ccttgtccag catctcaact gccccagcac ccaggcggga agctagaagc cggtcgaagg
      901 ccagcgggct gccgcctcgc tggacgtggc cgaggatggt gactcgcagc tcgaagccca
      961 gttgatcgtg gtaggtgttg aaatgattca gcagcgcttc cgcattcagc ttggctcctt
     1021 ccgccacaac cacgatggcg tgaggcttgc cgcgggcgta agcggaacgc aactcagcgg
     1081 ccacctggtc agggtccgtg tccacctcgg ggattagcac tacctcagca ccgccggcga
     1141 ttccggtcat cagcgccaga tagccgctgt ctcgccccat cacctccacc acgaaggccc
     1201 tgtggtggga ggaggcggta tccttcagcc tgtcgacggc ctccagcgcg gtgttcaggg
     1261 cggtatcgac gcctatggtg atatcggatc ccagcagatc gttgtctatg gtggacgcga
     1321 tgccgacaac ggggaagccc atctgcgaca gggcataggc cccggtctga gagccgttcc
     1381 cgccgatgac gaccagcgcc tctacccccc gcttggccaa ctcaaccatt gcctgccgtt
     1441 gcccctcttg ggtccggaac agggggctac gggcgctgcc caggacggtt ccgcccttct
     1501 ggatgatgcc tccgacgtcg cgggctgtca ggggggacat ctcgccggca atcaggcccg
     1561 cgaaaccgtg tctgaccccc agcacctcca ttccacgctc caatccccct ctgaccaccg
     1621 acctgatggc cgcgttcatt ccgggggcgt caccgccgct ggtgagcact gcgatccgtt
     1681 tcatggaccc c
//
LOCUS       DATALS010000535         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_129078, whole genome shotgun sequence.
ACCESSION   DATALS010000535 DATALS010000000
VERSION     DATALS010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129078"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2539)
                     /locus_tag="VHS06_11955"
     CDS             complement(<1..2539)
                     /locus_tag="VHS06_11955"
                     /inference="COORDINATES: protein motif:HMM:NF039643.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEX2988867.1"
                     /translation="MRRFVVSLILLALGLSVAWGAGDTLLRAGFEKALDADEAAGKPQ
                     ATAAGAVATVPDRLGNSLFLGPKTGSLSYAAAGNIDLKQGSLIAWLQPGDWQVAGGPV
                     TLLGARQGADALALWWEPKTTTLFFAANGGKLAVSKPAYDWVQKTAHYVILTWAPGKL
                     AMYVDGQAAQPSKGASMPDVPADQLILGGDLGDRGTLTIRGITVLKSVLTPEQVQQRY
                     RVAMSGQVEHERPLLTVPQCTSAPKIDGKLDDAAWNMAGGTSGFIEIAGGVLSSLGTR
                     FYLTYDAKNIYIAFRCPSTNKPLAHKFDRDGGSPWDEDAVEMWLQPEVGFTGDYFHLV
                     LNAAGSLWDAKNQNAAWNGTAAWAADQGDKEWTAELVVPHASLETMAPTPGTKWQANF
                     CRDIGDGGTTNRNTAWAYTGGSGYAVWQMFGALQFLPGSPVAQVQGWTAQGTDSTVQL
                     QLANPGPKPAKIGATVTAFRAGMSEEAQDTEGGPWTVPAGQTMAVDLSSKSPNPIDQV
                     KLQVRNLDTNQPLLTQMVRAGTREPVSGTTVAAAPGAAAAPAAPPLTNEMLGAVLRER
                     EKWENNRIGITDKVPPPWTPMTVAGKTVSCWGRTYDYEGSLFPRQVKSQAQDLLAGPI
                     QLVAKIGGKEVIFQKADKQSLVPAPQQVDFDAQTAAGGLTARVTSHLEYDGCIKLTLT
                     LTAANNKPVEIQGLELRIPVRADRALYYHWFEATRDPRLTNAGALPKDGLKGHFKPLL
                     WLGDNDRGLCWFSESPKGWQNNDKESILRVERQADTAVMRICMADRPYVIASSWQTVF
                     GLQATPTRPTTPGWRDWLIPLNQTNPWGSWGPGFNNLSGTDDEGTLMP"
     gene            complement(3377..>4055)
                     /gene="thpR"
                     /locus_tag="VHS06_11960"
     CDS             complement(3377..>4055)
                     /gene="thpR"
                     /locus_tag="VHS06_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX2988868.1"
                     /translation="SRVKVKWVERENLHITLKFLGATPALMVPDLREVGEALAAELEP
                     FELQLRGVGAFPKLSRPQTIVVKTGTGADELTVLAKLLHQRLAEEALLVENDKKPFVP
                     HCTLGRVRQESGLGRLVEAYMAIVGSVWASIRSESLSATCDSPMFAMSSEREKSCRVS
                     PSSSRRMGKMLRSAISRSSVGGPGAAKISLPSWRMKMPGAVPQQFSMCEAPSGASACL
                     RLLSARG"
BASE COUNT          644 a         1266 c         1415 g          730 t
ORIGIN      
        1 tgggcatcag cgtgccctcg tcgtcggtgc cgctgaggtt gttgaaacct gggccccatg
       61 agccccaggg attggtctgg ttaaggggga tgagccagtc gcgccagccg ggtgtggtcg
      121 ggcgggtggg cgtggcctgc aggccgaaga cggtctgcca ggagctggcg atgacgtagg
      181 gccggtcggc catgcagatg cgcatgacgg cggtgtcggc ctgacgctcg acgcggagga
      241 tcgactcctt gtcattgttc tgccagccct tgggcgattc gctgaaccag cagaggccgc
      301 ggtcattgtc gccgagccag agcaggggct tgaagtggcc cttgaggccg tctttgggca
      361 gggcgccggc gttggtgagg cgcgggtcgc gcgtggcttc gaaccagtgg tagtagagcg
      421 cgcggtcggc gcggacgggg atgcggagct cgaggccctg gatttcgacg ggcttgttgt
      481 tggcggcggt gagggtgagg gtgagcttga tgcaaccgtc gtattcgagg tgggaggtca
      541 cgcgcgcggt caggccgccg gcggcggtct gggcgtcgaa gtccacctgc tggggggccg
      601 ggaccaggct ctgcttgtcg gccttctgga agatcacttc cttgccgccg attttggcca
      661 cgagctggat cgggccggcg aggaggtcct gcgcctgcga cttgacctgc cgcgggaaga
      721 ggctgccttc gtagtcatac gtgcgacccc agcagctgac ggtcttgccg gcgacggtca
      781 tcggggtcca cggcggcggg accttgtcgg tgatgccgat ccggttgttc tcccacttct
      841 cgcgctcgcg gaggacagcg ccgagcatct cgttggtgag gggcggggcg gccggagcgg
      901 ctgcagcgcc cggggcggcg gcgaccgtag tgccgctgac gggctcgcgg gtgccagcgc
      961 gaaccatctg cgtgagcagg ggctggttgg tgtcgaggtt gcggacctgc agcttgactt
     1021 gatcaatcgg gttgggtgac ttgctggaga ggtccaccgc catcgtctga cccgccggga
     1081 cggtccacgg accgccctcg gtgtcctggg cctcctcgga catgccggcg cggaaggcgg
     1141 tcacggtcgc gccgatcttc gccggtttgg ggcccggatt ggctaactgc agttgcacag
     1201 tcgagtctgt tccctgcgca gtccagccct gcacctgcgc cacggggctg ccggggagga
     1261 actgcagcgc gccgaacatc tgccagacgg cgtagccgct gccgccggtg taggcccagg
     1321 ccgtgttgcg gttggtggtg ccgccgtcgc cgatgtcgcg gcagaagttt gcctgccact
     1381 tcgtccccgg cgtcggggcc atggtctcga ggctggcgtg agggacaacg agttccgcag
     1441 tccattcctt gtcgccctgg tccgctgccc aggccgcagt gccgttccag gctgcattct
     1501 ggtttttggc gtcccagaga cttcccgcgg cgttcaggac gaggtgaaaa tagtcgccgg
     1561 tgaagccaac ctcgggctgg agccacatct cgacggcgtc ctcgtcccag gggctgccgc
     1621 catcgcggtc gaacttgtgg gcgaggggct tgttggtcga ggggcagcgg aaggcgatgt
     1681 agatgttctt ggcgtcgtac gtcaggtaga agcgggtgcc gaggctggag aggacgccgc
     1741 cagcgatttc gatgaagccg gaggtgccgc cggccatgtt ccaggccgca tcgtcgagct
     1801 tgccgtcgat cttcggcgcg gaggtgcact gcggcacggt cagcagcggc cgctcatgct
     1861 cgacctggcc ggacatggcc acgcggtagc gctgctggac ctgctcaggg gtcaggacgg
     1921 acttcaggac cgtgatgccg cggatggtga gggtgccgcg gtcgccgagg tcgccgccga
     1981 ggatgagctg atcggcaggg acgtcgggca tggaggcgcc cttgctcggc tgagcggcct
     2041 gtccatcgac gtacatggcg agcttgccgg gcgcccaagt gaggataacg tagtgcgcgg
     2101 tcttctggac ccaatcatag gcgggcttgc tgacggccag cttgcccccg ttggcggcga
     2161 agaagagcgt ggtggtcttc ggttcccacc acagggccag cgcgtcagcg ccctgccggg
     2221 cgccgagaag cgtcaccggc ccgccggcca cctgccagtc accgggctgg agccacgcga
     2281 tgaggctgcc ctgcttgagg tcgatgttcc cggcggccgc atagctgagg gagccggtct
     2341 ttgggccgag gaagagcgaa ttgcccaggc ggtcaggaac ggttgctacg gctcccgcgg
     2401 cggtggcctg aggcttgccg gcagcctcgt cggcgtccag cgccttctca aagccggcgc
     2461 ggagtagggt atccccggct ccccaggcca ccgataggcc cagcgccagc aggattagcg
     2521 atacaacgaa tctgcgcatt gtcacggtca gtcctcctga gaaagccagc atcgtctttg
     2581 ttaacaagcg gaaccgtcgt catggtccgc gatgtgtata ttgcgacaga ggcggccgga
     2641 gacctgcctg agctcttagt cccgaaggga ctgccgacaa tttgccacca gcggaagctg
     2701 gtggaagggc gcgattcggg cctcctaaag cccccgaagg gggcgcacga gcttaggccg
     2761 cctcttcgag gcttgtaggg gaggatgggt aggcgcagac caccagcttc cgctggtggc
     2821 aaggtatcgg acgtcccctt tggggactgg cggaagcgga actcgccacc gcggacgggc
     2881 aagacgcccg tgctacgcga ggtggtgaga ggcaccgggt ttcagttgac gcatacggct
     2941 acatctacta caatgacggt agtacagatg tttgataagc gggttccaca ggcccggggg
     3001 tgtgggggcg ctgctggctt ctccaccgta aatgaaccac gaaaagcaga ctaaacttga
     3061 aagctgaaat catctcagtt ggcaccgagt tgctgctggg cgagattgtt gataccaatg
     3121 cctcctacct gtcgcaaaaa ctggctgaga tggggatcga cgtccacttc cgccatacgg
     3181 taggggacaa tctcgcacgg ctcactgaag ttatcaccac cgctctctca cgcgcggatg
     3241 tggtcattct caccggcggg ctggggccca ccgaagatga cctgacacgt gaggggattg
     3301 cggaggccac cggtcacccc ctcgttcgcg ttccagagtc cgagcagcac ctccgcgagt
     3361 tcttcgccgc ccgcaactac cccctggccg atagtaacct caagcaggcc gatgcccccg
     3421 agggcgcctc gcacatagaa aactgctgcg gcaccgcgcc cggcatcttc atgcgccatg
     3481 agggcaggct gatcttcgcc gctccggggc cgccgacaga actgcgggag atggctgagc
     3541 gcagcatctt ccccatcctg cgcgaggaac tgggcgagac acggcagctt ttctcgcgct
     3601 cgctactcat ggcgaacatc ggcgagtcgc aggtcgcgga cagactctcg gacctgatcg
     3661 atgcccagac cgaccctacg attgccatgt acgcctccac cagccgcccc agcccgctct
     3721 cctgcctgac ccgccccagc gtgcagtggg gcacgaaggg cttcttgtca ttctccacca
     3781 gcagcgcctc ttcagcgagc cgctggtgca ggagtttggc cagcacggtc agctcgtcag
     3841 cgccggtacc cgtcttcacc acaatcgttt gcggccgcga cagcttggga aaagccccca
     3901 ccccccgcag ttgcagctca aacggctcca gctcggctgc cagcgcctcc ccgacctcgc
     3961 gcaggtccgg caccatcagc gccggcgtgg cccccaggaa cttcagcgtg atgtgcaggt
     4021 tctcgcgctc cacccacttc acctttacgc ggctt
//
LOCUS       DATALS010000536         4790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_129513, whole genome shotgun sequence.
ACCESSION   DATALS010000536 DATALS010000000
VERSION     DATALS010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129513"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VHS06_11965"
     CDS             <1..715
                     /locus_tag="VHS06_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX2988869.1"
                     /translation="LAVPEWQRAALVPLLPEATYIPLLDGKEAQQGANLRAVMDTLPA
                     CNALAIGPGLGQGEGIVSLVLGVLEGNRGGPKVRTVVDADGLNALATVDRWWERIDPG
                     NVVTPHPGEMSRLVGIPTVEVNANRADVALDAARRWGQVVVLKGAFTVVADPDGAAWI
                     NPAAMPALGSGGTGDVLTGLLAGLMAQGASPLDAARAAVYLHTAAAELTLASSATDRL
                     LASDLLTSIPRAICDLACL"
     gene            complement(712..1122)
                     /locus_tag="VHS06_11970"
     CDS             complement(712..1122)
                     /locus_tag="VHS06_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX2988870.1"
                     /translation="MKSEQSGAGFFVGFLLGGVVGAAAALLITPRSGDETRDTIMERG
                     IELKVKAEEVAARAMDEADDLFTKGRTVLDSQRYRIQEAVEEGKEAAAQKKAELLSKY
                     RVAKETGEAPQSEPSLADEIPPRTEHPTEPEQEA"
     gene            complement(1119..1466)
                     /locus_tag="VHS06_11975"
     CDS             complement(1119..1466)
                     /locus_tag="VHS06_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988871.1"
                     /translation="MTATARDVAIIILCAETIVLMAVLLFIAWKGYQLIKLFQQKAEQ
                     YSSAGLSLMETAKQTAETASGTATTVKGSAEFISDTVVNPVVQVVSAVTGAKSFVAAL
                     FRLPNSTRYGGRR"
     gene            complement(1496..1801)
                     /locus_tag="VHS06_11980"
     CDS             complement(1496..1801)
                     /locus_tag="VHS06_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988872.1"
                     /translation="MIEEYTLRLLNTLTPAISRVPPPYYVGTLLGIINCCVFYLRFGR
                     GLKLFLPYLIIGAAGALVGLTVGRQLPDSGPMLGEVNVIVTSLAAWICLFVARSLRL"
     gene            2003..3724
                     /gene="recJ"
                     /locus_tag="VHS06_11985"
     CDS             2003..3724
                     /gene="recJ"
                     /locus_tag="VHS06_11985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX2988873.1"
                     /translation="MSERLWRLAQRLPDVVPQGLEGWPPLIRQLLYNRGLVDPAEITE
                     FVYPENGSHDPFLLRDMDTAVNRVFSALRQEELIAIYGDFDVDGLTSAALLLEVLQSD
                     ALQGHVITYLPHRGKEGYGLNPEAIRWLAEQGVRLLITVDCGIGGDGEIRLATQLSMD
                     VIVTDHHRISEGLPPAAAVLNPRREDCPYPFRDLAGVGVAYKLAEALLSRVWDLATAR
                     QRLMPQMDLVALGTVADLAPLVGENRFMVKSGLQQINRGERLGLRCLLDQAGYAGRTV
                     DAESIAYIIGPRINAAGRMGDARLGLDLLTCSSPEEGARLAAELESANKERQAATAAA
                     LNVAREELSHLGELPPALVLAGDYPSGVVGLVASRLAEEYRRPSFVIELGEAECRGSG
                     RGLPGFDVLRALSGASELLMRYGGHVQAGGFALEPAQLPELRDRLEAAAYEQMGTEVP
                     PQELLLEAALGPGKVGPALYEQLQLLEPYGIGNPRPLFYGSKLFVRDARVVGNNHLKL
                     WLSDQSGSSPAIGFGMGGEEYAFARAGARVDCAFTLSRNERNGTIGYEMMLKGLKPAG
                     QAGPLLA"
     gene            4073..4774
                     /locus_tag="VHS06_11990"
     CDS             4073..4774
                     /locus_tag="VHS06_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988874.1"
                     /translation="MSAELENSEVGVRLAEGKAEATGPKGKGERRAERRFEPSRYLRK
                     LGGKDYLEVKWRLLWLRTEHPDAVMQTEMVKLEEGFALFKARVAIPGAGESTGWGSET
                     RQDFFDYIEKAETKALGRALAALGYGTQFCEDFDFAAGSAGNAHVVDSPVSSPAVEAS
                     TPQPQRPTPIMQTNSSLATPAQLKLIYLTASRELHLSEEELEQRCQAQYGRLPIHLTK
                     REASEFIDALKRQSA"
BASE COUNT          881 a         1367 c         1616 g          926 t
ORIGIN      
        1 cctggccgtg cccgagtggc agagagcagc gctggtgccg ctgttgccgg aggcgaccta
       61 tattcccctg ctggacggga aagaggctca gcagggggct aacctccggg ctgtgatgga
      121 taccctgccc gcatgcaacg cgttggctat cggtccgggg ctgggccagg gcgaggggat
      181 tgtgtcgttg gtgctgggcg tcctggaggg taaccggggt ggcccgaagg tgcgtaccgt
      241 cgtggacgcc gatggcttga atgcgctggc caccgtggac cgatggtggg agcggatcga
      301 ccccggcaat gtcgttaccc ctcatccagg cgagatgtcc aggctcgtgg ggataccgac
      361 ggtggaggtc aatgccaaca gggcggacgt ggcgctggat gccgcgcggc gatgggggca
      421 ggtagtggtg ctgaagggtg cgttcaccgt ggtggccgac cccgacgggg cagcgtggat
      481 taaccccgcc gcgatgccgg cactgggcag tggtggcacc ggggatgtgt tgacggggtt
      541 gctggccgga ctgatggctc agggggcttc gccattggac gctgcgcgag cggccgtata
      601 tctccacact gccgcagctg aattgacact tgccagcagc gcgaccgacc ggttgctggc
      661 aagcgatctg ctgacgtcga tccccagggc gatctgcgac ctggcctgtc tctaagcttc
      721 ctgctcgggc tccgtggggt gctcggtcct gggtgggatc tcgtcggcta gggaaggttc
      781 agactgggga gcctctccgg tctccttggc tacgcggtac ttggacagga gttctgcctt
      841 cttctgcgcg gcggcctctt tgccctcctc cacagcctcc tgaatcctgt agcgctggct
      901 gtccaacacc gtcctgccct tagtgaagag atcgtccgcc tcgtccatgg ccctggcggc
      961 gacttcctct gccttcacct tgagctcaat tcctcgttcc attatggtgt cgcgtgtctc
     1021 atcgccgcta cgcggggtga tcaacagcgc cgcggccgcg ccgacaacgc cacccaacaa
     1081 gaagccaacg aagaagccgg ctccactctg ttctgatttc acctgcgacc tccgtacctg
     1141 gtcgaattgg gcaaccggaa gagcgctgcc acaaagctct tggccccggt aaccgcgcta
     1201 actacctgaa ctacagggtt gacgacagtg tcgctaataa actccgcgga gcccttcacc
     1261 gttgtagctg tgccactggc ggtttcggca gtttgctttg cggtttccat cagcgagaga
     1321 ccggcgctac tgtactgctc tgccttctgc tggaacagct tgatcagctg gtatcccttc
     1381 caggctatga agagcagcac agccatgagg acaatggttt cagcacacaa gataatgatg
     1441 gcgacgtcgc gggcggtcgc tgtcatgttc cactaccccc aatcgaacaa tgctatcaca
     1501 gcctcaggga gcgcgcaacg aagaggcaaa tccaagcggc caggctggta acgatgacgt
     1561 tgacttctcc caacatggga ccggagtctg gaagttgcct gcccacggtg aggccgacca
     1621 gcgcccctgc tgccccaatt atcaggtagg gaagaaagag tttgaggccc cggccgaagc
     1681 ggaggtagaa cacgcagcag ttgatgatcc ccaatagcgt gcccacgtag tagggaggcg
     1741 gcaccctgga gatcgccggt gtaagggtgt tcagtaggcg gagcgtgtac tcttcgatca
     1801 ttctcgtcgg acccacaaaa ggggggccgg atctccgccg cacccctggc ctgcattcat
     1861 tcgaacgaat cgtcaggaag aagcttttgt cgaatgtagc acatggcaga agtgggcgtc
     1921 aaccaacggc ggttttccgg gtgactgcga gcgccggtac tgatagaatc gcgggactga
     1981 tatatagaga agtgggtgtc ggatgtctga acgactttgg cgcttggctc agcggctccc
     2041 cgacgtggtt ccccaagggt tggaagggtg gccaccgctg attcgccagc tactgtacaa
     2101 ccgggggttg gttgaccccg cagagataac tgagttcgtc tatcccgaaa acggcagcca
     2161 tgatccgttc ctgctcaggg atatggatac cgcggtcaac agggtgttca gcgccttgcg
     2221 acaggaggag ttgatcgcga tctacggcga tttcgacgtg gacggcctca cctccgccgc
     2281 gctgctgctg gaggtgcttc agtcggatgc gcttcaaggg cacgtcatca catacctgcc
     2341 tcatcgcggt aaggaggggt atggcctcaa tcctgaagca atcagatggc ttgccgagca
     2401 gggcgttcgg ctgctcatta ccgtggattg tggcatcggg ggcgacggcg agatcagact
     2461 ggcgacgcag ttgagcatgg acgtgatcgt cacagaccac caccgcattt ccgaggggct
     2521 tcccccggcc gccgcggtgc tgaaccccag gcgagaggac tgcccatacc cattccgcga
     2581 tctggccggc gtaggggtgg cgtacaagct ggccgaggcg ttgctctcca gggtgtggga
     2641 tcttgcgacg gcgcgacagc ggttgatgcc ccagatggac ctggttgcgc tgggcactgt
     2701 ggccgacctg gcgccgcttg taggcgaaaa ccggttcatg gtaaagagcg gactccagca
     2761 gattaaccgc ggggagcggc tcggcctgcg atgtctattg gatcaggcag ggtatgccgg
     2821 acggactgtc gacgcggaga gtatcgcgta catcatcggg ccgaggataa acgcggccgg
     2881 caggatgggc gatgcccggt tgggcctgga cctcctgacc tgctcatcgc ccgaggaggg
     2941 tgcaagattg gccgccgaac tggagtctgc caacaaagag cggcaggctg cgaccgcggc
     3001 tgcgctgaac gtcgcccgag aggagttgtc tcacctgggt gagttgccgc cggcactcgt
     3061 gctggccggc gattacccgt caggagtagt agggctggtg gcttctcgcc ttgccgagga
     3121 gtaccgccga ccatccttcg tgatcgagtt gggcgaggcg gagtgtaggg ggtcagggcg
     3181 cgggcttccg ggtttcgacg ttctcagggc tctctccggc gcctcggagt tgctgatgag
     3241 atatggcggg cacgtgcagg ccggcgggtt cgcgctggag cctgcacagc ttcctgagtt
     3301 gcgcgaccgg ctggaggctg ccgcgtacga gcagatgggg acagaggtac cgccccagga
     3361 attgctgctg gaggcagcct tgggaccggg gaaggtgggg ccggcgctgt acgagcagtt
     3421 gcagttgctg gagccgtacg gcattggaaa cccgaggccg ctgttctacg gatcaaagct
     3481 gttcgttcgg gatgcgcgcg ttgtgggaaa caaccacctg aagctgtggc tcagcgatca
     3541 gagcggcagc agccctgcaa ttggcttcgg catgggcggc gaagagtacg cctttgccag
     3601 ggcaggggca cgagtagact gtgccttcac cctatctcgc aacgagcgga acgggaccat
     3661 tggctacgaa atgatgctaa agggactgaa accggccggc caggctggcc cacttcttgc
     3721 ttaatagcgc ggtcgaaggg atcgagatcg gataccttcg actttctcct cctcccacag
     3781 tttggcgggg ccggcccgag aaggccggcc ccgccaaaag gccccctaca ctacgcaggg
     3841 catagagcct gcactctttt ccttgagatc cccgtcacgt ggccattttg gggtgcctct
     3901 tgacagaatg tgcggattcc gtataattgg cgctagaaca tctgttctgt ttcgcttcga
     3961 ctgagtgcgc ggcagtgaga aaatcgggtc tggggtgcag cgcctgacgg ggtttggtgc
     4021 gtctccagat tcgcaacttc tctctgctac ctactagaaa gggacgagtg ccatgagcgc
     4081 cgagttggag aacagtgagg tgggtgtccg gttggccgag gggaaggcgg aggccacggg
     4141 gccaaagggc aagggcgagc ggagggcgga gcgccgcttc gagcccagca ggtatctccg
     4201 aaagctgggg ggcaaggatt acctggaggt caagtggcgg ctgttgtggc tgcgcaccga
     4261 gcacccagat gcggtaatgc agacggagat ggtaaagctc gaggaggggt ttgccctttt
     4321 caaggcgcga gttgcgatcc cgggcgccgg tgagtccacc ggctggggat cggagacgcg
     4381 gcaggacttc ttcgactaca tcgaaaaggc tgagacgaag gcgctcggtc gcgcgctggc
     4441 cgctctaggc tacggcacgc agttctgcga ggatttcgat tttgccgcag gctctgcggg
     4501 taacgcccac gtggtagact caccggtctc ttcccctgcg gtcgaggcct ctacacccca
     4561 accacagcgt cccaccccga ttatgcagac caacagctcg cttgccacgc ctgcccagct
     4621 gaagctgatc tacttgacgg cctcacggga gttgcacttg agcgaggagg agttggagca
     4681 gcgttgccag gctcagtatg gtcggctgcc gatccacctg accaagagag aggcatcgga
     4741 gttcatcgac gctctgaagc ggcagtcggc atagccggag gaagtcgtgg
//
LOCUS       DATALS010000537         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_131122, whole genome shotgun sequence.
ACCESSION   DATALS010000537 DATALS010000000
VERSION     DATALS010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131122"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..972)
                     /locus_tag="VHS06_11995"
     CDS             complement(16..972)
                     /locus_tag="VHS06_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2988875.1"
                     /translation="MEKLLSETARRATGYLEGLDSRSVAPSAEAIAGLARLGGPLPEE
                     PAGAEEVLRLLDEAGSPATMASAGGRYFGFVIGGSLPATLAANWLAGAWDQCAGLHAL
                     SPAAAAIEQVTLRWLKEVLPVPAGAGGGFVTGATMGNFTALAAARHAILAAEGWDVET
                     QGLFGAPPLTVIVGNEVHVSLLKALSMLGLGRDRVTRVPVDDQGRMRADALPEISGPA
                     IVCIQAGNVNTGAFDPAREICAAAHKAGAWVHVDGAFGLWAGAAPARAYLVDGVEDAD
                     SWSVDAHIPSACRRGQARCSRRSIAPPPARAPVPARTPSSPS"
     gene            complement(1661..1975)
                     /locus_tag="VHS06_12000"
     CDS             complement(1661..1975)
                     /locus_tag="VHS06_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988876.1"
                     /translation="MGFEQYHEPANELSEKTRTFARMMVSMIEEAEAIDWYEQRLSVE
                     KDAQARAIMRNAQQEEFKHFGMDLEFLLRRMPKWKAILQEILFKEGDIVEAGEEAEED
                     AK"
     gene            complement(2021..2452)
                     /locus_tag="VHS06_12005"
     CDS             complement(2021..2452)
                     /locus_tag="VHS06_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEX2988877.1"
                     /translation="MQSRVINQQGRIWFLVLANGEDVMPALRRFAEEHGIEGATFTGL
                     GAFEDSVVSWFNWETKEYEPVQAHGQAEVLSIVGSISLGLDGKQMVHAHAVLGRPNGS
                     VVGGHILEAKVRPTLEVTLVESAEALKRKTDAETGLALLAL"
     gene            complement(2711..4357)
                     /locus_tag="VHS06_12010"
     CDS             complement(2711..4357)
                     /locus_tag="VHS06_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX2988878.1"
                     /translation="MRLHVHLSSDWILDTEMREHRPDEEVDFCIVGTGAGGGVMAQRL
                     ARYGFSVVALEAGEWHDTETGMVSDEAGSGQLYWTDLRISGGEEPLELGANNSGRGVG
                     GSTVHYAAFAPRFHPSDFRVKTADGVAADWPMGYEDLEPYYEMMEREYPVSGPSRYPW
                     GKPHGYPYAPLEAGTAGQVLIEGCVKLGIPVVAGGPVAIPAGRVGKRPHCIMRGFCLL
                     GCKVGAKSSILVSHIPDAVEHGAEIRTGCMAYEVVVRDDGRVGSVRYYRTLDDGRVVE
                     EEQRAKAVVVAGYAIETPRLLLNSKSSRFPDGLANSSGKVGKYLMAQAGPVVWGRFDA
                     PIRQYKAPPACACTEEYYETDPRNDFVRGYALQTVSPLPIAMSHLLTEDGAFGEELLA
                     RMQDYNHYAAIGVLGEILPDERNYVSIHPTEKDHFGIPTSYVHFNLFENDKRMTSAGI
                     ARAKEVLGAAGARETWAVRRYAHLVGTCRMGSTAEESVVDEWCRSWDVPNLYICDGSV
                     LPTQGSANPALTISALAARAADRIRSAARRGELGAGTGGR"
BASE COUNT          662 a         1785 c         1225 g          816 t
ORIGIN      
        1 ccgtagtgtc gtggttcaac tgggagacga aggagtacga gccggtacag gcgcacgggc
       61 aggcggaggt gctatcgatc gtcgggagca tctcgcttgg cctcgacggc aagcagatgg
      121 tatatgcgca tccaccgacc acgaatcggc gtcctcgacc ccgtccacca ggtaggcgcg
      181 tgccggcgct gcgcccgccc atagcccgaa cgccccgtcc acgtgcaccc acgcgcccgc
      241 tttgtgcgcc gccgcgcaga tctcacgcgc cgggtcgaac gcccccgtgt tcacattgcc
      301 tgcctggatg cagacgatgg ccggccccga gatctcgggc agggcgtccg cgcgcatcct
      361 cccctggtcg tccaccggca cgcgcgttac cctgtccctc ccgagcccca acatgctcaa
      421 tgccttgagc aaacttacat gaacctcgtt gcccacgatc accgtcaggg gcggggcgcc
      481 gaacaagccc tgcgtctcga cgtcccatcc ctcggccgcg agtatcgcgt ggcgggcggc
      541 ggccagggcc gtgaagttcc ccatcgtggc gcctgtcacg aaccccccgc cagctcccgc
      601 gggcaccggc aacacctctt tcagccagcg taatgtcact tgctcgatgg ctgcggcggc
      661 cggcgacaga gcatggagcc ccgcgcactg gtcccacgcc cccgccagcc agttggcggc
      721 cagcgtggcg gggagcgaac ccccaattac gaacccgaag tagcgcccgc ccgccgacgc
      781 catcgtcgcc ggcgagccgg cctcgtccaa caggcgcagc acctcctcgg caccggccgg
      841 ttcttcgggt agcggccccc caagccgcgc gagacccgcg atcgcctctg cggagggcgc
      901 cacgctacgc gagtccagac cctccagata gccggtggcg cgccgcgccg tctcactcaa
      961 cagcttttcc atcgatactc ctccccgtag agccactcgc gcttgccggg cgctgcgcgg
     1021 gccgcctttg gcgtattgcc ccccatcata gcaccccgcg caagggagac attgccgccg
     1081 aagcgtccaa tatgcgcccc cgccacccgc cacccgcccc gtcacttcct tctgggatgt
     1141 gggtcgcccc gtcacccctc ccacggggtg tgcggcgtgc gcacggcgtt cccccaatcc
     1201 tcccccgccg gtcaccgcgc aacccgctcg gtccgcagac tcgcccctcc cgtggggtat
     1261 gggcatggcg ctctcccaat cctccccccg ttagtcatct ggcagaccct ttccggctgc
     1321 cgagctccaa ctccacatgg ggtgtggggc atgggcatgc cgctccccaa tccttccccc
     1381 attagtcatc gaccggccag ggccgcttac ccgcgcgtta ggcccacggg gtgtggggta
     1441 tggcatggcg ctacgcgcca tgcgcatacc ccacgtctaa taaatcaccc cctctcccct
     1501 tttggggaga gggggcaggg ggtgaggggt ccttcccctc cccagcggtc ggctccaggg
     1561 cacagccccc agcaggggca cgggggcgcc agcccccgag gataatctct ttcaccccct
     1621 cttcacttct gggggcaggg ggtgaggggt ctcacatccc ctatttcgcg tcctcctcgg
     1681 cctcctcgcc ggcttctaca atgtcgccct ccttgaacag gatctcctgc agaatcgcct
     1741 tccatttggg catgcgccgc agcaggaact caaggtccat cccgaagtgc ttgaactcct
     1801 cctgttgcgc attgcgcatt atcgctcgcg cctgcgcgtc cttctcgaca gacaatcgct
     1861 gctcatacca gtcgatcgcc tcggcctcct ctatcatcga caccatcatc cgcgcgaaag
     1921 tccgagtctt ctcggacagt tcgttcgccg gctcgtgata ctgctcgaat cccacttgcc
     1981 acctcctgat tagttcgccg cccgtcttct tggccggccc ctacaatgcc agcagtgcca
     2041 gccccgtctc cgcgtccgtc ttccgcttca gcgcctcggc cgactccacc agcgtcactt
     2101 ccagggtcgg ccgcaccttt gcctccagga tgtggccgcc cactaccgac ccattgggcc
     2161 tgcccagcac ggcgtgcgca tgtaccatct gcttgccgtc gaggccaagc gagatgctcc
     2221 cgacgatcga tagcacctcc gcctgcccgt gcgcctgtac cggctcgtac tccttcgtct
     2281 cccagttgaa ccacgacact acggaatcct cgaacgcgcc aagccccgtg aaggtcgcgc
     2341 cttctattcc gtgctcctcg gcgaaccgcc gcagcgccgg catcacgtcc tctccgttcg
     2401 ccagcaccaa gaaccagatc ctcccttgct gattgatcac tcgagactgc acccgttcct
     2461 ccttatcaac ccatgtctcg gcggcgcgcc gctccttgaa cgcgcccggc ctctattcac
     2521 ggcacattcg cgccccgctc atttgacggg cgatatgtcg ctccctgccg gccgtcttcc
     2581 cggcgcggcg agtcgccggc cgctatcagc ctgtcgcgag gagtttcccg catgtcaggt
     2641 agcaagacac gttccccggc cttccgcgat ccctcttgcc actcgccttt ccggagggcg
     2701 gacgcacctc tcaccgtcca cccgtccccg cgcccaactc tcccctccgg gccgcgcttc
     2761 gtatccgatc ggccgcacgg gccgccagtg ccgatatggt gagcgccggg ttcgccgagc
     2821 cttgcgtcgg gagcacgctt ccgtcgcaga tgtacaggtt cggcacatcc cagctccggc
     2881 accactcgtc cacaaccgac tcttccgccg tcgagcccat cctgcacgtg cccacgaggt
     2941 gcgcgtaccg tctcaccgcc caggtctccc tggccccggc cgcccccagc acctccttgg
     3001 ctctggcgat gcccgcgctg gtcatcctct tgtcgttctc gaacaggttg aagtgtacat
     3061 atgacgtggg gatcccgaag tggtcctttt cggtcgggtg gatcgacaca tagttgcgct
     3121 cgtcgggcag gatctccccg agcaccccga tggccgcgta gtggttatag tcctgcatcc
     3181 gggccaacag ctcttccccg aacgcgccat cctcggtcag caggtgcgac atggcgatgg
     3241 gcagtggcga gacggtctgc agagcgtatc cccgcacgaa gtcgttccgc gggtctgtct
     3301 cgtaatactc ctcggtgcag gcacacgccg gcggcgcctt gtactgcctg atcggcgcgt
     3361 cgaaccgtcc ccataccacc ggcccggcct gcgccatcaa gtacttgccg accttcccgc
     3421 tcgagttcgc cagcccatcc gggaaccggc tcgacttcga gttcagcagg agccttggcg
     3481 tctcgatcgc gtagcccgcc accaccaccg ccttcgcccg ctgctcctcc tccaccacgc
     3541 gcccgtcgtc cagcgtcctg taatacctga cgctccccac tctcccgtca tcgcgcacca
     3601 ccacttcgta ggccatgcac ccggtcctta tttcggcgcc atgctccacc gcgtccggta
     3661 tgtggctcac cagtatgctg ctcttcgccc ccactttgca cccgagcaga cagaaccctc
     3721 gcatgatgca gtgcggccgc tttccgaccc tccccgccgg gatggccacc ggtccccccg
     3781 ccaccaccgg tatccccagc ttcacgcacc cctctatcag cacctgcccc gccgtcccag
     3841 cctccagcgg cgcgtacggg tagccgtgag gtttccccca cgggtagcgc gaaggacccg
     3901 acaccgggta ctcccgctcc atcatctcgt agtatggctc gagatcctcg tatcccatcg
     3961 gccagtcggc ggccaccccg tcggctgtct tcactcggaa gtctgagggg tggaaccgcg
     4021 gagcgaacgc ggcgtagtgt accgtgctcc cccctacccc ccggcccgag ttgttcgccc
     4081 ccagctccag cggctcttcc cctccgctga tccgcagatc ggtccagtag agctgccccg
     4141 agcctgcttc gtccgacacc atgccggtct ccgtgtcgtg ccattccccc gcctccagcg
     4201 ccacgaccga gaagccgtac ctggcgagac gctgggccat caccccgccg cccgcgcctg
     4261 tccccacgat gcagaagtcc acctcctcat ccggccggtg ctcgcgcatc tcggtgtcca
     4321 gtatccagtc cgacgacagg tgtacgtgca gcctcatcgc gaccccctgt cgtgctcccc
     4381 ttccacctcc cgcgcttccc acggctcggg cgccccgtgg ttcagggcga agtacccccg
     4441 agggtacgcc gggcccccga accctatctc gtcccacgag taggggtg
//
LOCUS       DATALS010000538         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_131798, whole genome shotgun sequence.
ACCESSION   DATALS010000538 DATALS010000000
VERSION     DATALS010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131798"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..965
                     /locus_tag="VHS06_12015"
     CDS             174..965
                     /locus_tag="VHS06_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX2988879.1"
                     /translation="MSHIHIPDGVLPVWLVGAGWLLAIIVLGLVSLRVRHTEPGRQLP
                     LLGVMSALMLVGMSTEIIPIGYHINLSVLAGIVLGPAMSFLAAFIVNLILALFGHGGI
                     TVVGLNALVVGVEASLGYYLFSAVWSVLRGRQRTPALSAGIAAFIALVLSSLLMVGVV
                     ALSAIGPSAQASASLPESLSFQNPFEHGLIGSEIGRAEEHQADATGNSVDVATFAKLV
                     ALFGIAGWIAESLLTGAIVGFVHRVRPDLVGGRSRLDLLREKRVG"
     gene            972..2747
                     /locus_tag="VHS06_12020"
     CDS             972..2747
                     /locus_tag="VHS06_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2988880.1"
                     /translation="MDISAIDYWANRGTSGLHSSSALAKGGAALAFVASVVISNSPLL
                     LAALYLLLLAGVFASRLPATKLVAIALYPTIFALLFAASQWNGDFTRPATILLKALTA
                     AQAMVLLITTTPYPDIFAAVGRVMPRLISDGLFVTYRSFFLLLREMGRAMTALRLRGG
                     LRKGDYVGNARNVGRALGMLLVRAIDLSERLYSVLRVRGYRGRLEAGTRWRTPTRHDV
                     APIVAGAFALTLAIAARVSPSLWAAANGFVLVAAAAFAAIAFMTSRGREALADGSGTD
                     KREGVEAIMRHNSELGNRDNSGQHSHGAHPVPGEVARVSCVRHVYPDGTEVSLCGLDF
                     VAHRGERVVILGPNGAGKSTLLNHLLGLLKPTDGEVVVLGHDPATDFLAIQPKIGVLL
                     QDVDEQILGPTVADDVAFGARNAGYGERSVAMARRVMEQLGILHLADKVPHYLSGGQK
                     RKVALAGALVTDPDLLVLDEPFEGLDPRSRNELVWLLTELHRERGLTMVITTHHVDLL
                     PILADTVYVLADGGRIVTKGTPEEVFSQPDVLRESSIEAPILTILFHKLKEEGIDLGM
                     PVDVEDAARRLRDLRMEHSGPARSS"
     gene            2878..2985
                     /locus_tag="VHS06_12025"
                     /pseudo
     CDS             2878..2985
                     /locus_tag="VHS06_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
     gene            complement(3023..3112)
                     /locus_tag="VHS06_12030"
     CDS             complement(3023..3112)
                     /locus_tag="VHS06_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2988881.1"
                     /translation="MITCSITGYGSEGSYKDRTAFDLCVQSLL"
BASE COUNT          546 a          945 c         1019 g          651 t
ORIGIN      
        1 atcatcatca tgccatctgc tccggctgcg gcctcgtcgt ggacctccgt ggctgcgacg
       61 ttgaacgcgt agcggagcga gaggctgtgc aggccggctt tgccatcacc ggccaccggc
      121 tcgagtacta cgggttgtgc agcgcctgcc ggttgaaggg agagatctag catatgtcgc
      181 atattcacat ccccgatggc gtcctccctg tctggttggt cggcgcgggc tggctgctgg
      241 caatcatagt tctggggttg gtctcgttgc gcgtgcgcca tacggaaccg ggaaggcagc
      301 tcccgctgct gggagttatg tccgcgctga tgctcgtggg catgtcgacg gaaatcattc
      361 cgattggata tcacattaac ttgtcggtgc tggccggcat cgtgctcggt ccggcgatga
      421 gcttccttgc cgccttcatc gtaaacctga tcctggcgct gtttggccac ggtggcatca
      481 cggttgtggg cctgaacgcc ttggtcgtag gcgtcgaagc ctcgttgggc tactacctct
      541 tcagtgcagt ctggtcggtt ctgaggggaa ggcagcgtac ccccgcgctt tcggccggaa
      601 ttgccgcgtt catcgccctc gttctcagtt cgctgctgat ggtcggcgtc gtggccctca
      661 gcgcgatcgg gccatctgcc caggcatccg cctcacttcc ggagagcctc tccttccaga
      721 acccgttcga acacggtttg atcgggtcgg agattggcag ggccgaggaa catcaggcag
      781 acgccacagg gaactccgtc gacgtcgcca cgttcgctaa gctggtggcg ctctttggaa
      841 tcgcaggctg gatagccgag tcgttgctga cgggcgcgat tgtggggttt gtgcaccggg
      901 tgcggccgga tctagtcggg ggcaggagtc gtctcgatct gctccgcgag aagcgagtgg
      961 ggtaggtctg gatggatatt tcagccatag attactgggc gaacagaggg acgagcggac
     1021 ttcattcctc atccgcgctt gccaaggggg gcgccgctct cgccttcgtc gcctcggtcg
     1081 tgatttccaa tagccctctt ctgctggcgg cgctgtacct tcttctcctt gctggagtgt
     1141 tcgcgtcgcg actgcctgcg acaaagctgg ttgctatcgc gctttatcct acgatctttg
     1201 cactgttgtt cgcggcgagc cagtggaacg gagacttcac gagacctgct actatcctgc
     1261 tgaaggcgct cacggcggct caggcgatgg tgttactaat cactaccacg ccgtaccctg
     1321 atatctttgc ggcagtcggc cgggtcatgc ccaggctgat ttctgatgga ttgttcgtga
     1381 cataccgctc tttctttctt ctgttgaggg agatgggccg tgcgatgacc gcgctccggc
     1441 ttcgtggcgg tctccgcaaa ggggactacg tcggcaacgc ccgcaacgtc ggccgggcgc
     1501 tgggaatgct gctggtgcgt gccatagacc tgtctgaaag gctctactcg gtgctgcggg
     1561 ttcgcggcta ccggggcagg ctggaggcgg gtacgcgatg gcggactccg accaggcacg
     1621 acgtggcccc gatagtggcg ggtgcattcg ccctcaccct cgcgatcgct gccagggttt
     1681 cccccagcct gtgggcagcg gccaacggct tcgtactggt ggcggcggct gcattcgcgg
     1741 caatcgcgtt catgacctcg cgcggaagag aagcgctggc ggatggaagc ggaactgaca
     1801 agcgggaagg agttgaagcg atcatgcgcc ataactcaga actgggaaat cgggacaact
     1861 ccggccagca ctcccatggt gcgcatcctg tccctggcga ggtcgcgcgg gtcagttgcg
     1921 tccggcacgt ctaccccgac gggacggagg taagcctctg cgggctcgac ttcgtcgccc
     1981 accggggcga aagggtggtc atcctggggc cgaacggggc gggcaagagt accctcctga
     2041 accacctgct cggtctgctc aaacccactg atggtgaagt ggtcgtcctt ggccatgacc
     2101 ctgccaccga ctttctcgcc attcagccga agattggcgt cctgctgcag gatgtcgacg
     2161 agcagatcct gggaccgacc gtggccgacg acgtggcgtt cggggcgcgg aacgctgggt
     2221 acggcgagag aagcgttgcc atggcgcgga gagtgatgga gcagctaggc atcctgcatc
     2281 tggcagataa ggtgccgcac tatctgtccg gtggccagaa acgaaaggtc gctctagccg
     2341 gcgcgctggt aactgaccct gacctgctcg tgctcgacga gccgttcgag ggcctggacc
     2401 cacgctcgcg caacgagctc gtctggctgc tcaccgaatt gcaccgcgag cgcggactta
     2461 ctatggtcat caccacccac cacgtggacc tgcttcccat cctcgcagac actgtgtacg
     2521 tcctggctga tggaggacga atcgtgacca agggtacgcc cgaagaagtc ttctctcagc
     2581 ccgacgtgtt gagagagtcc tcgatcgaag cgccaatcct cacgatcctc ttccacaagc
     2641 tcaaggagga ggggatagac ctcggaatgc cagtggacgt cgaagacgcg gccaggaggc
     2701 tgcgcgactt gcgaatggag cactcaggac ccgcaagatc atcttgacac caccccgggg
     2761 tggtcccaca attgccgcgc aaatcctcat tctcgagggg gagcaatgag caggacactg
     2821 agtggagtcc gcgttctcga cctcacggcc gcgctctcgg ggccctacgc agccatgatg
     2881 ctgggggatc tcggggccga agtcctcaag atcgaacctc tcgagggcga gcccttccgt
     2941 gtgatgggcc catactacag gaacgattgg agcgcctact tcgtaggctg caaccgcaat
     3001 aagcttggcg tcgctctcaa cctcaaagta gactctggac gcagaggtcg aacgcggtcc
     3061 tgtccttgta gctaccctcg gagccgtacc cggttatgga gcaggtgatc acgcgcgggt
     3121 tggcttaacg agcaacttgc cagtcaggcg ctggagggca c
//
LOCUS       DATALS010000539         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_131951, whole genome shotgun sequence.
ACCESSION   DATALS010000539 DATALS010000000
VERSION     DATALS010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131951"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..785
                     /locus_tag="VHS06_12035"
     CDS             381..785
                     /locus_tag="VHS06_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988882.1"
                     /translation="MFQVGRTRLIKASAVGVAVALVGGYVLALLGQGGGFFGFGSLLV
                     LLLGYLVGEAVSRGADRRISRELVWLAGGLTVLGVVAGRVALVLSRLPSALPLGLRLE
                     AAVNFGLMDLLGNLFGLLFLVLAVVVATSRIR"
     gene            807..1841
                     /locus_tag="VHS06_12040"
     CDS             807..1841
                     /locus_tag="VHS06_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2988883.1"
                     /translation="MNRNRLLEVLPGFRDKRVLVVGDLILDEYLVGRPTRISREAPVL
                     ILEYEKRYALPGGGTNPARNLSALGAEALMVGIVGTDEAGSELRSLLGSAGVRTDGIV
                     VDPERPTITKTRILAQDRDIVRQQVVRMDRLPKSAPSPATLRRLVDFLEEKVPQVDAV
                     LLSDYKNGVISPQVVEACSRLAKQYGKLVTADSQGDLHRFSGFSLVKANQSETAATLG
                     CELDCDDAFENACQRLLDELGAKVVMITRGANGMSVMESGGRPTHISVANRSEVFDVT
                     GAGDTVIAVATLALAAGARAVSAAHLANYAAGLVVRKIGNATTNIQELSEVIVSSQFP
                     KVDPEQEMMG"
     gene            complement(2333..2620)
                     /locus_tag="VHS06_12045"
     CDS             complement(2333..2620)
                     /locus_tag="VHS06_12045"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX2988884.1"
                     /translation="MRTRVHVFVSGLVQGVNFRMATRREAEMRGVTGWVANAADGRVE
                     AVFEGEAPDVQRLVDWCHHGPSRARVDRVEVQTEPYVGEFDSFDIIHGPGW"
BASE COUNT          479 a          801 c          888 g          550 t
ORIGIN      
        1 cttggagaat gagggcaagc gccatccctg aggactgagc gagcaggaca gcattgcggc
       61 gtcaagccgc cgcactccaa aacggtcgct ccgacgaaag ccgtggtacc ggatatgggt
      121 gacgcatgcg tcgcctctac gggaacacag gcccggagtg ggtgcggaaa gtgctaaact
      181 gaggacaatc ttgttttctg tggggaccta atttggccgt cgagagcgag caaagagcgg
      241 ctccgcgttg cgccaaccat ccggataccg agacccatct ccgctgcagc cgctgcggga
      301 agccgatatg tgtgcgctgc gtgatccaga cgcccgtggg cggacgctgc cgcgagtgcg
      361 cggacttgag gaagcccccg atgttccagg ttgggagaac ccgcctgatc aaggcttcgg
      421 cagtaggtgt ggcggttgca ctcgtagggg gctacgttct cgcgctgctg gggcagggtg
      481 gagggttctt cggcttcggt tccctgctgg tgctgctgtt gggctacctc gtcggcgagg
      541 cggtcagccg cggcgcggac cgtcgcatct cccgcgagtt ggtctggctg gccggcggtc
      601 tcaccgtgct gggcgtggtg gctgggcgag tcgcactggt gctcagcagg ctgccgtcag
      661 cacttccgct gggtctgagg ctggaggccg cggtcaactt tgggctgatg gacctgctgg
      721 gcaacctgtt tgggttgctg tttctcgttc tcgcggttgt cgtggcgacc agccgcatca
      781 gatagtgccg tcgccggagg ttgcagttga ataggaaccg gcttctggaa gtgcttcctg
      841 ggtttcggga caagcgggtt ctcgtggtgg gggacctgat cctggacgag tatctggttg
      901 gcagacccac caggatctct cgcgaggccc ccgttctcat cctggagtat gagaagcggt
      961 atgccctccc cggcggcgga accaatcccg cgcgtaacct gagcgccctg ggtgccgagg
     1021 cgctgatggt gggtatcgtg gggacggacg aggccggttc ggaactgcga tccttattgg
     1081 gctcggcagg agttcgcacc gacgggatag tggtggatcc tgagcggcct accataacca
     1141 agacccgtat cctggctcag gatagggaca tcgtcaggca gcaggtagtt cgcatggaca
     1201 gattgccgaa gtctgctccc agccccgcaa ccctgcggcg tttggtcgac tttctggagg
     1261 agaaggtgcc acaggtcgac gcggtactgc tctccgacta caagaacggc gtgatctccc
     1321 ctcaggtggt ggaggcctgt tctcggctgg ccaagcagta tggcaagctc gttactgccg
     1381 actcgcaggg cgaccttcat cgctttagcg ggttcagcct ggtgaaggcc aaccagtccg
     1441 agaccgctgc caccctcggt tgtgagttgg actgcgacga cgcctttgag aacgcatgtc
     1501 aacgtcttct ggacgagtta ggcgcgaaag tcgtgatgat cacgcgtgga gccaacggta
     1561 tgtcggtcat ggagtccgga ggacggccca cccacatctc ggttgccaac cgaagcgagg
     1621 tgttcgacgt gaccggcgca ggcgacaccg tcatcgccgt ggcaacgctg gcgctggcgg
     1681 ccggtgctag agcagtgtcc gcggcccatc tggcgaacta tgcggcaggc ttggtcgttc
     1741 ggaagatcgg caacgccacg acgaacatcc aggagttgag cgaagtaatc gtcagttctc
     1801 agttcccgaa ggtggatcca gagcaggaga tgatgggcta ggaccgagga gccggccttt
     1861 cgatctgata gggcaggccg tcacgaggga ttggctactc ctccggcgaa tccgggaatt
     1921 cagtaccctc gcaaccgtcg gtcgaggata cctttgctga ggcctttccg tttcttctcc
     1981 cccgggcagt accctttgcc tgctcatggc cattctggca catgccgcac cccgggtggt
     2041 tcttgcgcca acaaccgttg acggcacgaa gacaacgctg ctgctcctcg tcggtcaccg
     2101 gaggggtgtc ccagaggcag atgatctgat agagcagatg ctctccgggc gcgcctgcgt
     2161 cggagtccgc ggaccagttc tcgcggtaga agcagcatgc gcccttgtgg cgacggtagt
     2221 gcgaccgatc tggttgtgga gttactggtt tctcttgctg ctcagaaatg gttagtctcc
     2281 cccagccgaa ttagcccaat tgtaccactg gccggccgtt cacggaaacc gtctaccaac
     2341 cggggccgtg gatgatatcg aagctatcga actctccgac gtaaggttcg gtttgcacct
     2401 ccaccctatc gacacgggct ctggatgggc cgtgatggca ccagtccacc aatctctgca
     2461 cgtcgggtgc ctcaccctcg aagactgcct ccacccgacc gtctgcggcg ttcgcgaccc
     2521 agccggtgac cccacgcatt tcagcctcgc ggcgagtcgc catccggaag ttcactccct
     2581 gcaccagacc tgatacgaag acgtgaaccc gagtcctcac ccttccatcc tcgaggccaa
     2641 aattgacgcc ccatggtagg atttgcgacg ctgggagtca actgatggca tctgggttgc
     2701 gtttgtccgg tagggcag
//
LOCUS       DATALS010000540         3412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_132044, whole genome shotgun sequence.
ACCESSION   DATALS010000540 DATALS010000000
VERSION     DATALS010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132044"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VHS06_12050"
     CDS             <1..425
                     /locus_tag="VHS06_12050"
                     /inference="COORDINATES: protein motif:HMM:NF026049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX2988885.1"
                     /translation="QFFDAVDTASACLKMFAGILATARVKDQRTREAAAGGFSTATDI
                     ADYLVRKGLPFRKAHEVVGRLVRHCLDRACRFEDLTLDEIRSFSPEFDEDVLTIDVQA
                     SVNARDVPGGTAPNRVRDAIAEGRRLLAATETWLASLP"
     gene            complement(686..928)
                     /locus_tag="VHS06_12055"
     CDS             complement(686..928)
                     /locus_tag="VHS06_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988886.1"
                     /translation="MSDIAQVVGALLILVAFALAQFGRMGQRSLGYLVLNIVGSGILA
                     VVAYVGSQPGFLLLEGAWALVSLWSLGGVIRQSRAA"
     gene            1039..1803
                     /locus_tag="VHS06_12060"
     CDS             1039..1803
                     /locus_tag="VHS06_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX2988887.1"
                     /translation="METDRRNAIQRAADLISRANRIVVFTGAGVSTESGIPDFRSPGG
                     IWDRYKPVDFSEFVASKQARERYWTRSRETYPVLAAASPNPAHLAIAELERRGKLDCV
                     ITQNVDGLHQKAGNSPDRVIELHGNTHWVVCLSCAHRSPRSEWQETLKSGTLEPRCPD
                     CGGILKVLTVSFGQAMPERETREAIRRSQLCDLFIVVGSTLVVFPAADMPLYALESGA
                     RLIVVNLTPTYVDDRADVVICDKAGPALSAIVRESE"
     gene            complement(1795..2721)
                     /locus_tag="VHS06_12065"
     CDS             complement(1795..2721)
                     /locus_tag="VHS06_12065"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX2988888.1"
                     /translation="MKVLVVGPGAMGTLFAGLLVEGGHEVSLLGRRPEVIKSISENGI
                     VIERDGGARAVPVRAVLRAQDAGRCDLVLMFVKAYSTLQASRDAAAAAGEDTVVLTLQ
                     NGLGNVDAIASVFGRRRVLAGVTAHGATLIRPGNTRHAGVGETSIGELDGALTPRLSR
                     LAEAFRSSGVEVEASNCVECLIWGKLIVNAGINPLTAILHVPNGGLLERSETRDVMAA
                     AASEATAVAAARGMPLPYEDALVRIEEVCRLTAANRSSMLQDVERGVQTEVDYINGAI
                     VREGVALGVPTPVNWTLTQLIKGIEPPREVSF"
     gene            complement(2728..>3412)
                     /gene="panB"
                     /locus_tag="VHS06_12070"
     CDS             complement(2728..>3412)
                     /gene="panB"
                     /locus_tag="VHS06_12070"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX2988889.1"
                     /translation="LGYESTVPVTMEDILHHTRAVVRGARSTFVVADLPFMSYQVDVA
                     EALRNSGRLIKEGGADAVKLEGGASMAETVRAIVRAGIPVMGHLGLTPQTATMLGGLK
                     VQARDAEAAKALVADAIALEEAGAFAIVLEAIPTQLASLVTERLSIPTIGIGAGPKCD
                     GQVLVIHDMMGLFQRFTPKFVKKYADLGGEMARALATYCQEVDESAFPAAEHSFNMKE
                     AEVARLIEE"
BASE COUNT          671 a         1156 c          973 g          612 t
ORIGIN      
        1 agcagttctt cgacgcagtg gacaccgcct ccgcatgcct gaagatgttc gccggcatac
       61 tcgccactgc acgcgtgaag gaccaacgaa ccagagaggc agccgctgga ggcttctcca
      121 cggcgacgga tatcgccgac tacctggttc gaaaggggct gccgttccga aaggcgcacg
      181 aggtcgtggg aagactggtg cgccactgcc tcgacagggc ttgccgcttt gaggatctga
      241 cgctcgatga gatcaggagc ttctcccccg agttcgacga agacgtcctg accatagacg
      301 tgcaggcatc cgtgaacgcc agagacgtgc ccgggggaac ggctccaaac agggtcaggg
      361 atgcgatcgc cgaaggaagg cgcctcctcg cggctaccga aacctggctc gcctctctgc
      421 cttagaacgt cctgggtcca tggagggcga ggctcccgcc ctccaactca gtccgcccgc
      481 gaacacgccc ctatctgcgt ctcttcttcc cgatgctctc caagggaacc gcacccttct
      541 gcaagggcac gtcctgcggg cgtcccctcc tggtagccgc cgacaccatg cgccgtatca
      601 acagtgccgc gccaaaacca aacaaaagaa agagccctgg gaccaggtat tccatagcac
      661 ctccaaccat cggcggttcc ccgcctcagg ctgctcgtga ttgtctgatc actccgccga
      721 ggctccacaa ggacaccagt gcccaggccc cttccagcag aaggaacccg ggctggctcc
      781 caacgtaggc cacgacggcg agaattccag aaccaactat attcaacaca agatagccga
      841 gagagcgctg ccccatccgg ccaaactggg ccagcgcgaa tgcaaccagt atcaacagcg
      901 cgcccaccac ctgggcgatg tcactcaatg aacctcctcc gatattcctg atgcctccgg
      961 agacacgccc agttgccagc atcgtgccat caggtgggat gatacaatcc agtctcagct
     1021 tcgtaacgag gtgctcccgt ggagacagac cgcagaaatg ccattcagcg agctgcagac
     1081 ctgataagtc gcgcaaaccg catcgtcgta ttcacgggcg cgggcgtgag caccgagtcg
     1141 ggcatccccg acttccgtag tcccggcggt atctgggatc gttacaaacc cgtcgacttc
     1201 tcggagttcg tggcaagcaa acaggcccga gagcgctact ggacccgcag cagggaaaca
     1261 tacccggtcc tcgccgccgc gagtcccaat ccggctcacc ttgcgatcgc ggaactggag
     1321 aggcgtggca aactcgattg cgtcatcacc cagaacgtcg acggtctcca ccagaaggcg
     1381 ggcaactcgc cggacagggt aatcgagctt cacggcaaca cccactgggt ggtctgcctc
     1441 tcctgcgccc accgatcgcc ccgttctgag tggcaagaga ccctcaagtc cggaaccctg
     1501 gaaccgcgat gcccggactg cggagggata ctgaaagtcc tcactgtctc attcggccag
     1561 gcgatgcctg agcgagagac tcgggaggca atacgcaggt ctcagttgtg cgacctgttc
     1621 atcgtagtgg ggtcgacctt ggtggtcttc cccgccgccg acatgccact gtacgcattg
     1681 gagtccggcg cgcggctgat cgtcgtgaac ctgactccta cctacgtcga tgaccgcgcc
     1741 gacgtcgtca tttgcgacaa agccggcccc gcgttgtccg caatcgtccg agaatcagaa
     1801 tgagacttct ctgggtggct cgatgccctt gatcaactga gtcagcgtcc agttgaccgg
     1861 ggtgggaacc ccgagcgcca ccccttccct cacgatggcg ccgttgatgt agtcgacctc
     1921 cgtctgaacc ccgcgttcta cgtcctggag catcgaggag cggttggccg ccgtaagacg
     1981 gcagacttcc tctatacgta ccagcgcgtc ctcgtacggg aggggcatgc ctctagccgc
     2041 ggccacagcg gtggcttcgc tcgctgcggc agccatcacg tcccgtgtct cgcttctctc
     2101 cagcagtcct ccgttgggaa cgtgaagtat cgctgtcaga gggttgatcc ctgcgttgac
     2161 tatcaatttc ccccagatga ggcactccac gcagttggag gcctccactt ctacccctga
     2221 ggaccgaaag gcctcggcga ggcggctcaa cctcggagta agggcgccat ccaactcccc
     2281 gatggaggtt tcgcctactc cagcatgacg ggtattccca ggccggatga gtgtcgcgcc
     2341 gtgggccgtt acgcccgcca ggacgcggcg gcgaccgaac acggacgcga ttgcgtcgac
     2401 gttccccaac ccgttctgca gagtaaggac aacggtgtcc tcaccggcag ccgccgccgc
     2461 atccctggag gcctggagcg tcgaatatgc cttgacgaac atcagaacca ggtcacacct
     2521 tccggcgtcc tgggccctaa gtacagccct gacaggaacg gccctggcgc ctccgtcacg
     2581 ttcgatgacg ataccgttct ccgagatgga cttgatcacc tcggggcgtc gccccaggag
     2641 cgatacctcg tgtcccccct ccaccaggag cccggcgaaa agcgtgccca tggcgccagg
     2701 cccaactacc agcaccttca ctcgcagcta ctcctcaatc agcctcgcga cctccgcctc
     2761 tttcatgttg aaggagtgtt ccgccgccgg gaaggcgctt tcgtctacct cctggcagta
     2821 ggttgccagg gcacgcgcca tctccccacc gaggtcggcg tacttcttga cgaacttagg
     2881 agtgaagcgc tggaagaggc ccatcatatc gtggatgacg aggacctggc cgtcgcactt
     2941 cggccctgct ccgatgccga tcgtcggaat cgacagccgc tcggtgacca gagatgcaag
     3001 ctgcgtaggg atcgcttcga gaacgatcgc gaatgcacct gcttcttcaa gcgctatcgc
     3061 atcggcaacc agggccttcg ccgcctctgc gtctcgtgcc tggaccttga gtccgccgag
     3121 catcgtcgcc gtctgcggag tcagtccaag atgccccatg acgggtatgc ccgcgcgcac
     3181 tatcgccctc acggtttccg ccatcgaggc gccgccctct agcttaacgg catcagcgcc
     3241 gccttccttg atcagcctgc cggagttgcg gagcgcctcc gctacgtcca cctgatacga
     3301 catgaacgga agatcggcga ctacgaaagt gctccgggcc ccacgaacca ccgcgcgggt
     3361 gtgatggaga atatcctcca tggtcactgg gaccgtggat tcgtatccga gc
//
LOCUS       DATALS010000541         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_132048, whole genome shotgun sequence.
ACCESSION   DATALS010000541 DATALS010000000
VERSION     DATALS010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132048"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(502..795)
                     /locus_tag="VHS06_12075"
     CDS             complement(502..795)
                     /locus_tag="VHS06_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988890.1"
                     /translation="MKARIRKRQMQSVLPVHPAYHHPRRQPVGYLLKQLEHRHQGQEQ
                     RRYGSPPRRLIQGGELVLIRQELVEVLADLEVDIARGQDYLPDPGRVWRNVPC"
     gene            complement(1094..2308)
                     /locus_tag="VHS06_12080"
     CDS             complement(1094..2308)
                     /locus_tag="VHS06_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2988891.1"
                     /translation="MSEIILGSQNNDESEEKPIVEKPEEPVATPRPEHLNTWPDPLPA
                     YPTIGIDPTAMFVFVGIVLATIVLILFLWRVRSVMMVLFLGVILAESIRPSVRWLARR
                     GLPVPAAVAIPYLIILGTVAGIGAIVVPILSSEVIAFGEQLPGLISWGQSETVRMLQL
                     TGNPDLVNNLQQASGQLTERLVALTEFLVRLPITIAEVVIAITATLVISIYWLSLAPA
                     ALDEIFSIIPIAHRDRVWEALEEMGGRVGGYVRGTFVLALAIGVLVYPGLVALDVRYP
                     LILAAVAAFFELVPFIGPVLGAVPGVIVALIDSPTKAALAIVLYVVVQQIEAHLLAPN
                     IMGKAVGVAPLIVILALLSGSTIGGVVGAIVAIPAAAVAQVVVTQAILPVLQDRARRP
                     RPTPEDKGAHVP"
     gene            complement(2353..3390)
                     /locus_tag="VHS06_12085"
     CDS             complement(2353..3390)
                     /locus_tag="VHS06_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2988892.1"
                     /translation="MAATRAQDYLILAGQICLPRPEEDRDPKERASWLQLWLEALRYE
                     PGEPAESEDTVEELDRQWGKVRVQRLSVWPARPGTPAREDLQHAEQSNTTEFPMAGPV
                     PSAGAMLLPEPTATDLARIMQYLSTSPASEPAETLAAATERAAARARTIYPAAPRGDR
                     SRVPGRILGQLAHKALEQWRLPKRTPDLDRILENQAWNLGLTDESPIERAASQTMDLL
                     RRFERSDLYREMTAAGTTRRHEVPFSVPVGGRLVHGTIDALFRRPGGRWVVVDFKTEQ
                     VEGDREALQENIDANHITQVAIYALAITELVGHTPVVLLHYVRPGQTVPVPEADWQRQ
                     LKLLGVATMVT"
BASE COUNT          661 a         1105 c         1128 g          658 t
ORIGIN      
        1 ggtgactaac agggaccata gcgatcttcc cgcgtgccat atcgcagaca gcgccgggga
       61 catcggcgca cttccgttcc acgccccaac tgtccacgct ccctatccaa cccccttttc
      121 catagcggta cgggcggctt ctgcgacaat cccgtcgtgg cggcgtttcc ccaggctttc
      181 catacaggcc ctctctactc cctccagcaa ggtaggggcg gcgccttgtg cccgcccggc
      241 tggtgggtac accaccctct ccatgaactc gccgtcacca gccgtagagt ggcgccaatg
      301 ttcgttccag cacatgctgc ccggtacacg ccatgggcgg tggaagctgg caagatggtc
      361 gcacccacaa ccagggctag gacaagcgac tccgtcggtt ttctggacag ggatagcgga
      421 ttagcgatag cataaggata tggctgagat ggcttgatag caaggcccat cgggaggtgg
      481 aagatgtcgc tccagtggca gttagcacgg gacattccgc cagacacggc cgggatcggg
      541 caggtaatcc tgtccccggg cgatgtctac ctccagatcg gcgagcactt cgacgagctc
      601 ttgcctgatg aggacgagtt cgcccccctg tatgagacga cggggcgggg agccatatcg
      661 cctctgctcc tggccctggt gacggtgttc cagctgcttg agaagatacc cgaccggctg
      721 gcggcgagga tggtggtaag ccggatggac tggaagtacg ctctgcatct gccgcttgcg
      781 tatacgggct ttcacttctg tgacctctcg gccttccgcg gccgcctctt agagcatgag
      841 agtgagagga tggtgttcga ggggcttctt gggcggctta aggcgatggg gctgcttaag
      901 caggcaggga aggggaggac ggactccacg catgtgctgg ggctggtgga gaggctgagc
      961 gtggacagtt ggggcgtgga acggaagtgc gccgatgtcc ccggcgctgt ctgcgatatg
     1021 gcacgcggga agatcgctat ggtccctgtt agtcacccgg taagcaggcg agccggcgcg
     1081 cgggaagacc cggctagggg acgtgggcgc ctttgtcttc cggagtgggc ctgggccggc
     1141 gcgccctgtc ctgcaggaca gggaggatgg cttgcgtcac caccacctgg gccacggccg
     1201 cggcggggat ggctactatc gcgcctacta cacctccgat ggtagatcct gatagaaggg
     1261 ctaagatgac gataagaggc gcgacgccga ccgcctttcc catgatattg ggcgccaata
     1321 agtgggcctc gatctgctgc accacgacgt atagcactat cgccagggcg gctttagtgg
     1381 gcgagtcgat cagggcgact atcactccag gaacggcccc gagcaccgga ccgataaagg
     1441 gaaccagctc gaagaaggcg gccaccgcag ccagaatcag cggataccgc acatcaagcg
     1501 ccaccaggcc ggggtagacc agcacgccga tcgcgagggc gagcacaaag gtcccccgaa
     1561 catagccgcc gaccctgcca cccatctctt ccagggcctc ccagacgcgg tctctgtggg
     1621 ctatggggat gatagagaaa atctcatcca gcgcggcagg cgccaggcta agccagtata
     1681 tagagatcac cagggtggcc gtgatggcga taaccacctc cgcaatcgtg atgggcaacc
     1741 tgaccaggaa ctccgtgagc gccaccaggc gctccgtgag ctggcccgat gcctgctgca
     1801 gattgtttac cagatcgggg ttgccggtca gttgcagcat tctcacggtt tccgactgtc
     1861 cccaacttat tagcccgggg agttgctcgc cgaaagctat gacctccgat gaaaggattg
     1921 gaaccacgat cgcaccaatg ccggcgaccg tgcccaggat tatgaggtaa ggtatggcaa
     1981 ccgccgcggg caccggaagg ccgcgtcttg ccagccagcg cacggatggt ctgatagact
     2041 cggccaatat gacgcccagg aagagtacca tcattaccga tctgacgcgc cagaggaaca
     2101 gaatgagcac tatcgtggcg aggacgatgc ccacgaatac gaacatggcc gtgggatcga
     2161 tacctatggt ggggtaagcc gggagcgggt cgggccacgt gttgaggtgc tcgggacggg
     2221 gggtggcaac tggttcctcg ggcttctcta ctatcggctt ctcttcggac tcgtcgttgt
     2281 tctggctacc caggatgatt tcactcattg ataataagaa cccggagaca ccgccggact
     2341 aacaagtaca gcttatgtga ccatcgtcgc cactcccaac agtttgagct gtcgctgcca
     2401 atctgcctcg ggcacgggta cggtctggcc cggacgaacg tagtggagca ggacgacggg
     2461 cgtgtggccc accagctccg tgatggccag ggcatagatc gccacctgtg taatatggtt
     2521 ggcgtcgatg ttttcctgaa gtgcctccct atctccctcc acttgctcgg tcttgaaatc
     2581 gaccactacc catcgcccac cgggtcgccg gaacagggca tcgatggttc catgaaccag
     2641 ccgcccgccg accggcaccg aaaaaggaac ctcgtgccgg cgggtcgtgc cggccgcggt
     2701 catctcccgg taaaggtcgc tgcgctcgaa acgccggaga agatccatgg tctggctcgc
     2761 cgcccgctcg atagggctct catccgtcag gcccaggttc cacgcctgat tctcgagtat
     2821 gcgatcgagg tcaggggtcc ttttcgggag gcgccactgc tccagcgctt tgtgcgccag
     2881 ctgaccaagg attcttccgg gcacgcgcga cctgtcaccc cgcggcgcgg cggggtagat
     2941 ggtccttgcc ctcgcggcgg cgcgctccgt tgccgccgcc agcgtctccg ccggctccga
     3001 agccggtgaa gtagacaagt attgcataat ccttgccagg tccgtggccg ttggctcggg
     3061 caggagcatc gcaccggcgg aagggacggg gccggccatc ggaaactccg ttgtgttgct
     3121 ctgctccgca tgttggaggt cctccctggc cggggtgcct ggccgggccg gccataccga
     3181 caatctctgc acccttacct tgccccactg ccggtctagc tcttccaccg tgtcctcgga
     3241 ctctgccggc tccccgggct catatctcag ggcctccagc cacagttgca gccagcttgc
     3301 ccgctccttc ggatccctgt cctcctccgg ccgcggcagg catatctgcc cggcgaggat
     3361 aaggtagtcc tgggcgcggg tggccgccac gtacaggagc cttcgcttct ccgcccgcgc
     3421 cagctccggc ctcagtctcg ccggcttcat agcccgcgtt caggagctgc gcgatcagga
     3481 ggcgagagag ggagaagcga ttgtagaggc cgagggatcg gtccagatca tgactgtcca
     3541 caaggccaag gg
//
LOCUS       DATALS010000542         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_132868, whole genome shotgun sequence.
ACCESSION   DATALS010000542 DATALS010000000
VERSION     DATALS010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132868"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="VHS06_12090"
     CDS             complement(<1..1447)
                     /locus_tag="VHS06_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX2988893.1"
                     /translation="MDNFSNLMMGFSVALTWQNLAAAFLGAFIGTIVGSMPGVGASAG
                     IAMLVPVSFNYDATTALILMAAVYYGCMYGGTITSILVNLPGESASVMTCMDGYAMAK
                     KGQAGKALAVAQIGSFIWGTLGVLAIMLIGPPLAEFALSFGPPEYFALMFLGLTCLSA
                     MGEGSTVKALLMVALGLFFASMGTDPLAGAARFTFGNFMFLNGIDFIPAAVGLFAVGE
                     LLIEAETVVAMHFGKYSLREMILTKQDWKDAGGAWARGGLVGFLMGALPGAGATISSF
                     IAYFVERKFSKHPETFGTGEIKGVAAPEAANNAATAGAILHLLVLGVPGSGATAVMLG
                     ALMIHGLRPGPLLFTQNPEFVWGLIASMYIGNIVLLIMNMPLVPVFVAILRLPYSVLV
                     PIIITVSMIGVYSVNNSMFDVVAMFVFGILGYVFKKFQFPVAPLVLSLVLGEMLERSL
                     RQALAMSHNDPSIFVTRPIAAVLLAIALVSLL"
     gene            complement(1472..1933)
                     /locus_tag="VHS06_12095"
     CDS             complement(1472..1933)
                     /locus_tag="VHS06_12095"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX2988894.1"
                     /translation="MRKGDLVISTLLFFMGWWVLWQATMLPHLDLAGPGPEFVPNLVG
                     ASLVLLSAIMFVNTWRGSAVTPEGFIPDRAGIIRVVTMVVGLFFYVALLELVGYLVLT
                     FIYTFYTITAMSKYRWYTRAIVAAVITVTFYQGFVGFLEVPLPRGSIFGGS"
     gene            complement(1995..>3015)
                     /locus_tag="VHS06_12100"
     CDS             complement(1995..>3015)
                     /locus_tag="VHS06_12100"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX2988895.1"
                     /translation="APKAAEPTKPAAAAPTTAPAAAAPTAAPKAAEPAKAAAPAWTPE
                     KNVSFIVHAGAGGGSDIQARTMEKVITDEKLYPKSIAVENKTGGSGAVAFAYVAGKKG
                     DPYTWLTATTSFFTTPARGQVPLDPVKDFTMLARLSDDFFFVFVKTDSPFKTIQDVID
                     AAKKTPDGLKWGGTNVGSDDHIFYSKFAKATGTKFNFIPVPGGGDVVTSVLGGHVDVA
                     SGNPGEVMAQVQAGKLRALASSGDKPLPNYQQIPTLKSLNIDVLYQQTRMIAAPAGLP
                     AGVREYYAGLFKKMVASKGWKDYIQKNDQNENWMEGDTLDKYIKDTNESLKALIVELG
                     LVEKK"
BASE COUNT          599 a          935 c          960 g          521 t
ORIGIN      
        1 tcagcagcga caccagcgca attgccagca gcacggccgc gatgggcctg gtgacgaaga
       61 tggaggggtc gttgtgggac atcgccagcg cctggcgcag cgatcgctcc aacatctcgc
      121 ccagtaccag cgacagcacc agtggcgcca ccgggaactg gaacttcttg aaaacatagc
      181 ccaggatccc gaagacgaac atcgcaacca cgtcgaacat gctgttgttg acgctgtaga
      241 caccgatcat ggagaccgtg atgatgatcg gcacgagcac cgagtagggg agccgcagta
      301 tggcaacgaa gacgggcacg agcggcatgt tcatgatcag caacactatg ttgccgatgt
      361 acatgctggc gatcaacccc cagacgaatt ccggattctg ggtgaacagc agcggtccag
      421 gacgaagccc gtggatcatc aatgcgccca gcatgacggc ggttgcgccg gagcccggca
      481 cacccagcac cagtaggtgg agaatcgctc cggcagtggc cgcgttgttg gcggcctcgg
      541 gggccgcgac acccttgatt tcgccggtcc cgaaggtctc tgggtgcttg gagaacttcc
      601 gctcgacgaa gtaggcaatg aaggaggaga tggttgcccc cgcgccgggc agcgcgccca
      661 tcaggaagcc gacgaggccg ccccttgccc aggcgccccc cgcgtccttc cagtcctgct
      721 tcgtcaggat catctctcgc agcgagtact taccaaagtg catcgccacc acagtctcgg
      781 cctcgatcag caactcgccc acggcgaaca gccccacggc ggccgggatg aagtcgatgc
      841 cgttcaggaa catgaagttg ccgaaggtga agcgggctgc ccccgccagc gggtcggtcc
      901 ccatgctggc gaagaagagg ccgagcgcca ccatcaggag cgctttgacc gtcgagccct
      961 cacccatggc ggagaggcag gtgagcccca ggaacatcag cgcgaagtac tccggcgggc
     1021 cgaaggagag cgcgaactcc gccagcgggg gcccgatcag catgatcgcc agcacgccga
     1081 gagtacccca tatgaaggag ccgatctggg ccaccgccag cgccttgccc gcctgcccct
     1141 ttttggccat ggcgtagccg tccatgcagg tcataaccga cgccgactcg ccggggaggt
     1201 tgaccaggat ggaggtaatc gtgccgccgt acatgcaacc gtagtagacg gcggccatca
     1261 ggatgagcgc ggtggtggcg tcgtagttga agctcacggg caccagcatg gcgataccgg
     1321 cgctggcgcc gacacccggc atggacccca cgatggtgcc gatgaaggct cccaggaagg
     1381 cggccgccag gttctgccag gtgagggcca cagagaagcc catcatcagg ttggagaagt
     1441 tatccaagga tctattgctc ccttcccgag gctatgagcc gccgaagata ctacccctgg
     1501 gcagcggcac ttccagaaag ccgacaaaac cctggtagaa ggtgacggtg atgaccgcgg
     1561 caacgatggc ccgcgtgtac caccggtatt tcgacatggc cgtaatcgtg tagaaggtgt
     1621 agatgaaggt gaggaccaga tagcccacaa gttccaacag cgcaacgtag aagaacagac
     1681 ccaccaccat cgtgacgacg cggatgattc ctgccctatc tgggatgaac ccttcagggg
     1741 tgactgcgga gccgcgccag gtgttgacga acattattgc ggacagaaga acgagtgagg
     1801 ctcccaccag gttcggcacg aactcgggtc cggggccggc cagatccagg tgaggcagca
     1861 tggtagcctg ccacagcacc caccagccca taaagaacag taacgtcgag atcaccaggt
     1921 cgccttttcg catagatagc ccctcctaga atagactcgg ggggcgattc ccgccccccg
     1981 agccaccagg ttgcctactt tttctcgacc aggcccagtt cgacgatgag cgccttcagg
     2041 ctttcgttgg tgtccttgat gtacttgtcg agcgtgtcgc cctccatcca gttctcgttc
     2101 tggtcgttct tctggatgta gtccttccag cccttgctgg caaccatctt cttgaacagg
     2161 ccggcgtagt actcgcggac accggcgggc aggccggccg gcgcggcgat cattcgggtc
     2221 tgctgataca ggacgtcgat gttcaggctc tttagcgtcg ggatctgctg gtagttgggc
     2281 agcggcttgt caccggagga ggccagggcg cgcagcttgc ccgcctgcac ctgcgccata
     2341 acctcgccgg ggttgccgct ggccacgtcc acgtggccgc ccaggaccga ggtcaccacg
     2401 tcaccgccac ctggaaccgg aatgaagttg aacttcgtgc cggtcgcctt ggcgaacttg
     2461 ctgtagaaga tgtggtcgtc ggatccgacg ttcgtcccgc cccacttcaa gccgtcgggg
     2521 gtcttcttcg ccgcgtcgat tacgtcctgg atcgtcttga agggcgaatc ggtcttcacg
     2581 aagacgaaga agaagtcgtc ggagaggcga gccagcatcg tgaagtcctt caccggatcc
     2641 agcgggacct ggccgcgagc cggcgtggta aagaaggagg tggtggccgt caaccaggtg
     2701 taggggtcgc ccttcttgcc agcgacatag gcgaaggcca ctgcgccgct accgccggtc
     2761 ttgttctcca cagcgatgct cttcggatag agcttctcgt cggtgatgac cttctccatg
     2821 gtgcgggcct gaatgtcgga gcctccaccc gcacccgcat gcacgatgaa gctgacgttc
     2881 ttttcggggg tccaggccgg cgccgcggcc ttggccggct cggcagcctt gggcgctgca
     2941 gtcggtgcag cagcagcggg ggcagtagtg ggggcggcag cagccggctt ggtcggctcg
     3001 gcagctttgg gagcc
//
LOCUS       DATALS010000543         3995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_133011, whole genome shotgun sequence.
ACCESSION   DATALS010000543 DATALS010000000
VERSION     DATALS010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133011"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..482
                     /gene="fabZ"
                     /locus_tag="VHS06_12105"
     CDS             51..482
                     /gene="fabZ"
                     /locus_tag="VHS06_12105"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX2988896.1"
                     /translation="MIDIELIRDTIPHRYPFLLIDRILEVNEDGTACVGIKNITANEK
                     ILQGHLPGQAIFPGALLVEHMAQVGCYLLMTKPEAKGKLAYFAAIDNVRFKRSATPGD
                     TVRTKVELISGRRGIWKIKAESRVDGELVCRGNLTCALVDY"
     gene            607..1107
                     /locus_tag="VHS06_12110"
     CDS             607..1107
                     /locus_tag="VHS06_12110"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2988897.1"
                     /translation="MTLPYLKTKLIKALIAHFGDDDRRIDHALAVTSFAERILEREGG
                     DEQIVLAVGLLHDVGIKQAEEIHSYNTGKMQEQYGPPIARAILQTIGLPEDKIAEACA
                     IIGSHHTAAGVPGPNFPILWDADMIVNLADEMPDADPDKLRSIIEKSFRTQTGKALAI
                     QHLLPG"
     gene            complement(1119..1559)
                     /locus_tag="VHS06_12115"
     CDS             complement(1119..1559)
                     /locus_tag="VHS06_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988898.1"
                     /translation="MVWLIGVVTAVIGVTVGMAAAGLFGDPVGAAENDKIAQVVMWLS
                     FGLPFYLGMRWTGRLKPLWIWVACLLPAALFTGWGILDGNPFDNHGGYLDDELITIYI
                     LPTMGALALSSGVAFAQTGRWSVVGRLLAGTAVSILAVHAANSL"
     gene            2053..3447
                     /locus_tag="VHS06_12120"
     CDS             2053..3447
                     /locus_tag="VHS06_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2988899.1"
                     /translation="MLIRPVDEHKITELRSSFRGRILLPGDSDYDKARTIWNAMIDKH
                     PALIAQCADARDVMTAIAFTRENDLPGSVRGGGHSIAGNALCDNGVVIDLSLMRQVRV
                     DPESQTATAQGGVLWGEFDRATQQHGLATTGGLISTTGIGGFTLGGGMGWLERKYGLT
                     CDNLIAAEVVTADSKLVRASADENRDLFWAIRGGGGNFGVVTEFTYRLHPVGPMVLGG
                     MLIWPFSEADQLYQLYREYTADLTDDLTLMASPLTAPRAPFVPPEMQGKPCVAIIGCF
                     AGSVEDGNRVLAPLRDASPVADMFTEMPYVAVQSMLDPIAPAGIRNYWKSDYLAEITD
                     DFIQVLAEYFRGVPSPMTHVDVHHLEGAYGRVGEDETAFSHRDARYIVNIVSAWTDLA
                     DDQRNINWTRDLWNAIRPHSLGATYLNFVTNEGEDRVKASFGELKYHRLAQIKRKYDP
                     TNFFRRNHNIKPAE"
     gene            3780..>3995
                     /locus_tag="VHS06_12125"
     CDS             3780..>3995
                     /locus_tag="VHS06_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988900.1"
                     /translation="MSKAATYTDLVNLFNEFREFRPPKLREGIPDYTAAAMSEQHAQL
                     KLYQQRLADMDIADWPVSEQVDYHIVRA"
BASE COUNT          922 a         1393 c         1033 g          647 t
ORIGIN      
        1 gatataatca ctttgcaaac gaatcgagct cactggtcca ggagagaaca ttgatcgaca
       61 tagagctaat cagagacaca attccgcacc gctacccgtt cctgctcata gaccgcatac
      121 ttgaggtcaa cgaggacggc accgcgtgcg tcggcataaa aaatattacg gcaaacgaga
      181 agatcctgca gggccatctg cccggccagg caatcttccc tggggcgctg ctcgtcgagc
      241 acatggcgca ggtgggctgc tacctgctta tgaccaagcc cgaggcgaaa ggcaaactcg
      301 cctacttcgc agccatagac aacgtcaggt tcaagcgctc cgcaacgccg ggagacaccg
      361 tgaggaccaa agtggagctg atcagcggtc gtcggggcat atggaaaatc aaagccgaaa
      421 gccgagtaga cggcgagtta gtctgccgag gcaacctaac ctgcgcgctg gtggactact
      481 aacagcgaag cccctacagc tcaaatccct ccaggtatcc ctacctggag ggccgtatct
      541 gcgaagatac atcctgtggt ttcaggtccc ttccccgacg tgttgacgtt ctgactctta
      601 gactttatga ctttgcccta cctgaaaacc aaactgatca aagccctcat tgcccacttc
      661 ggcgatgacg accgccgcat cgaccatgcg ctcgccgtca ccagtttcgc cgaacgaatc
      721 ctcgaacgcg agggtggcga cgagcagatc gtgctcgccg tgggcctcct ccatgacgtg
      781 ggcatcaagc aggctgagga aatccatagc tacaacacag gcaaaatgca ggagcaatac
      841 ggcccgccca tcgcccgcgc cattctccag accatcggcc tgcccgagga caagatcgcg
      901 gaagcctgcg caatcatcgg ctcacaccac accgcagccg gcgtccccgg ccccaacttc
      961 ccgatcctgt gggacgcaga tatgatagtg aatctggctg acgaaatgcc cgatgccgac
     1021 cccgacaagc tgcgctcgat catcgaaaag agcttccgca cccaaaccgg caaagccctg
     1081 gccatccaac acctgctgcc cggctagcct gtcggcactc acagcgagtt cgctgcgtgc
     1141 actgccaaga tcgagaccgc tgtgcccgcc aggagccgac cgacaacgct ccagcgtccg
     1201 gtctgcgcga acgccacccc gctcgagaga gcgagcgcgc ccatggtcgg cagtatataa
     1261 atcgtaatca attcgtcgtc cagatagccg ccatgattat caaaagggtt gccgtctaat
     1321 atgccccagc ccgtaaacag cgccgcaggc agcaggcaag cgacccatat ccacaaaggc
     1381 ttcagcctcc ccgtccaccg catgcccaag tagaacggaa gcccaaagct cagccacatc
     1441 acaacctggg ctatcttgtc gttctcggca gctccgaccg gatcaccgaa cagccccgct
     1501 gccgccatac caacagtcac gccaatcaca gcggtcacca caccgataag ccagaccacg
     1561 atcatctgtc caaatgcatt acaagccctg cccaatgctc cgagaaccct gcctcgcacg
     1621 tcccctaccc ctgacccggg atccggggca gtgccgcccc catccggtac gctcgtcccc
     1681 caaagcccca atcgcaacgc cggccacctc ctcgcaactg ctgactgatc aggttattcc
     1741 acttcggcca ccaatccctc cagacacctt acctaaacgg cacactcagc atcctgagcc
     1801 ccatcgaagg gtgcgacctg cgaaggcaca ccctcccaac cccaagacat ctcgaaccag
     1861 caccgcgcca gtcctgagtg gcccgcaggg ctgtatcgaa ggacgacctg cgaaggtaca
     1921 gcgctgcaac tccaagctcc cttctgtaac ctataacctg tccactaccc cgcccgtttg
     1981 cccaagcccc cctcgcggga accatatgcc ctgtaggcga taccgccaaa gtgaacacgg
     2041 gaggacaagc gggtgttaat cagacctgtt gacgaacaca agatcactga actgagatct
     2101 tcattcaggg gtcgaattct tctgccggga gacagcgact acgacaaggc ccgcaccatc
     2161 tggaacgcga tgatagacaa gcacccggcc cttatcgccc agtgcgcgga cgcccgcgac
     2221 gtgatgaccg ccatcgcctt cacccgcgaa aacgacctcc ccggctcagt cagaggcgga
     2281 ggccacagca tagcaggtaa cgccttgtgc gacaacggcg tggtaataga cctgtcactg
     2341 atgaggcagg tcagggtcga tcccgagtcc cagacagcca cagcccaggg cggagtattg
     2401 tggggtgagt tcgaccgcgc aacccagcag cacggcctcg ccaccaccgg tgggctcatc
     2461 tcgaccaccg gcatcggcgg cttcaccctc ggcggtggaa tgggctggct ggaacgcaaa
     2521 tacggcctca cctgcgacaa cctcatcgct gccgaggtgg tcaccgccga tagcaagctg
     2581 gtccgcgcga gcgctgacga aaaccgggac ctgttctggg ccataagagg aggtggaggc
     2641 aacttcggcg tggtcaccga gttcacctac cgccttcacc cggtcggccc tatggtgctc
     2701 gggggaatgc tgatctggcc gttttcggag gccgatcagc tctaccagct ctaccgcgag
     2761 tatacggccg acctcaccga cgatctcacc ctgatggcgt cgcctctcac cgccccgcgg
     2821 gcgccgttcg tgccgccgga gatgcagggc aagccttgcg tggcgatcat cggctgcttc
     2881 gccgggtccg tcgaagacgg caaccgcgtc ctcgccccac tgcgcgatgc ctcgccggta
     2941 gccgacatgt tcaccgagat gccgtacgta gccgtgcaat cgatgctcga ccccatagcc
     3001 cccgcgggga tccgcaacta ctggaaaagc gactacctgg cagagatcac cgacgacttc
     3061 atccaggtcc tggccgaata cttccgcggc gtcccctcgc caatgactca cgtcgacgtc
     3121 caccacctgg agggcgccta cggcagagtc ggcgaggacg aaaccgcctt cagccacaga
     3181 gacgcgcgct acatagtcaa catagtcagc gcatggaccg acctcgccga cgaccagcgc
     3241 aacatcaact ggacccggga cctctggaat gcaatcagac cccactccct gggcgcaacc
     3301 tacctcaact tcgtaaccaa cgaaggcgaa gatcgcgtca aagcctcatt cggggagctc
     3361 aaataccacc gcctcgccca gatcaaacgc aaatacgacc ccaccaactt cttccgccgc
     3421 aaccacaata tcaagccagc ggaataggaa gggacggttc cgggttacac gttccgggtt
     3481 ccgggttggg aagggacttg tgattgagag gacgtgcctg cgaagccgcc acgcctggtg
     3541 tttcgggact tcagtcccga aaccgccgag cgcactcact caccgggccc agagagttgc
     3601 gtcggttgca ggcagccgac gccgcgaccg caggtcgcga gtcccttctc ccaacgctca
     3661 actcccttcg accatcaaac tatcaaacca tcagacggtc ccttccccct ccccgcattc
     3721 caaccaaaag tgctataatc accccatacc cggcgcaccg gccatccaag gagatgctca
     3781 tgagcaaagc agccacctac accgaccttg tcaacctgtt caacgagttc cgcgagttca
     3841 gacccccgaa actacgcgag ggcatacccg actacaccgc ggcggccatg agcgagcagc
     3901 acgcccaact caagctctac cagcagcgcc tggcggatat ggacatcgcc gattggcccg
     3961 tcagcgagca ggtagactac cacatcgtcc gcgcc
//
LOCUS       DATALS010000544         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_133041, whole genome shotgun sequence.
ACCESSION   DATALS010000544 DATALS010000000
VERSION     DATALS010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133041"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VHS06_12130"
     CDS             complement(<1..1105)
                     /locus_tag="VHS06_12130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2988901.1"
                     /translation="MTDNSGLRVISIGLDLTIALDDPSKGSRGDAHRRQAEYAELSAG
                     YTAIVKTPRSSRYRPYRWGDGHAVIPTLSASALNFPRDAYRLAAEVYRERGADLVVTQ
                     DPFSTGLAGYWLKKRLGIPLCIQVHNDMIGCEWWMRESRANRLMEPLGAWLVRRADTV
                     QVVSHTIRDRLTARGMASEKAWNIMTGAGIDATQFESADGASLRAQLLGDRYRRLVLF
                     MGRLVKQKDLPTLLKAASIVARKNPDTLFLLVGDGEERGYLERLVPGLGLETNLRIPG
                     GVGPTEAPTYFAACDLFVMSSVYEGKARALVEAACAAKPIVTTAVSGADEVVDEGKTG
                     YLVPLKDPSALADRILRVLDDPQRAAEMGQRGRA"
     gene            complement(1102..1548)
                     /locus_tag="VHS06_12135"
     CDS             complement(1102..1548)
                     /locus_tag="VHS06_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2988902.1"
                     /translation="MIVGGGPEEEYLRGLANDLGVSDRVVFVGSVPQSQVVLYLRACD
                     VFVLNTRYEGLSHTILECMDTGIPVVATAVGGNMELVEDGFNGFLVEVDDRKQIVSAV
                     RKVLYDPNVRENFIVRSKEKVKDASWDRLVDTTVGILDQVARGVSE"
     gene            1499..>2152
                     /locus_tag="VHS06_12140"
     CDS             1499..>2152
                     /locus_tag="VHS06_12140"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2988903.1"
                     /translation="MASPLRYSSSGPPPTINLFFRGEPLLHPHIVDMVKIARAAGIAV
                     HINTNATLLRGKLVEELLDAGLDKLTISFDAGEKEQYEQMRRGAKYEKTLENVLTFLK
                     AKAARGSAKPYFTIQVIRFFDPSQPRPTVPESFVHLFDGLPVDEFDPLWAHGWAGTMK
                     ATKDFKAAPYGHNYYPCNWLWKSLAICWDGKVATCCGDFGPADVFGDLNTDTLRGIWN
                     "
BASE COUNT          402 a          701 c          632 g          417 t
ORIGIN      
        1 atgcccggcc cctctgcccc atctcagcgg ctcgctgagg atcgtccagt acccggagta
       61 tacggtccgc cagggccgag ggatccttga gtggaaccag gtagccggtt tttccttcgt
      121 cgaccacctc gtccgcgccg ctcaccgcgg tcgtcacgat cggcttggcc gcgcaggcgg
      181 cctccaccag cgccctcgcc ttgccctcgt acaccgagga catcacgaag aggtcgcagg
      241 cggcgaagta ggtgggggct tccgttggcc cgaccccgcc gggtatcctg agattggttt
      301 ccagcccaag ccccggcacg agtctctcca gatagcctcg ttcctccccg tcgccaacca
      361 gcaggaagag ggtgtccggg ttcttccgcg cgactatgga tgccgctttc aacagcgtcg
      421 gcaggtcctt ctgctttacc agcctaccca tgaagaggac taatctacga tagcgatctc
      481 ccagaagctg ggcacgcagg gatgcgccgt cggcgctctc gaattgtgtc gcgtcgatgc
      541 ccgcgccggt catgatgttc cacgccttct cagacgccat tcccctggcg gtcaaccgat
      601 cgcgaatcgt gtggctgacg acctgcaccg tgtctgctct acgaaccagc catgccccca
      661 gcggttccat cagccggttg gcacgagact ccctcatcca ccactcgcag ccgatcatgt
      721 cgttgtgtac ctggatgcag aggggaatac ccagccgttt cttcagccag tagccggcca
      781 gtcccgtgga gaaggggtcc tgagtgacca ccaggtcggc acctctttcc cggtagacct
      841 cggcggcgag ccgataggca tcgcgaggga agttcagcgc ggaggctgag agggtgggga
      901 tcacggcgtg gccatctccc caccggtagg gccggtatct tgaagagcgg ggggtcttta
      961 ctattgcggt gtaccctgca gatagctccg cgtactcggc ctgtctacga tgggcatcgc
     1021 ccctggagcc tttcgatgga tcatccaggg cgatggtgag gtcgaggccg atagagatca
     1081 ccctcaatcc gctgttgtca gtcattccga tacacccctg gcgacctgat ccaggatgcc
     1141 cacggtggta tctaccagcc ggtcccagga tgcgtccttc accttttcct tggagcgaac
     1201 gatgaagttt tcgcgaacgt tgggatcgta cagcaccttc ctgacggcag agacgatctg
     1261 cttgcggtcg tccacctcca ccagaaagcc gttgaagccg tcctccacaa gctccatgtt
     1321 gccgcccact gctgtggcga ccactgggat gcccgtgtcc atgcactcca ggattgtgtg
     1381 ggagagtccc tcgtagcggg tgttcagcac gaagacatcg caggcgcgca ggtagagtac
     1441 cacctgggac tgggggacgc tccccacgaa gacgactcga tcgctaaccc caaggtcgtt
     1501 ggccagccct ctcaggtact cctcttccgg cccgcctccg acgatcaacc tgttcttccg
     1561 aggcgagccg ctgctccacc ctcacatcgt ggacatggtg aagatcgccc gtgcggccgg
     1621 catcgcagtt cacatcaaca cgaacgcaac cctccttcgg ggaaagttgg tggaggagtt
     1681 gctggacgcg ggactagaca agctgaccat cagcttcgac gccggtgaga aggaacagta
     1741 cgagcagatg cgccgcggcg cgaagtacga gaagacgctg gagaacgtcc tgaccttctt
     1801 gaaggccaag gctgcccgag ggtcagccaa gccctacttc accatccagg tcattcgctt
     1861 cttcgatcca tcacagccaa ggccgaccgt tcctgagtcc ttcgtccatc tcttcgatgg
     1921 gctgccggta gacgagttcg acccgctctg ggcccacggt tgggcaggga cgatgaaggc
     1981 aaccaaggac ttcaaggccg caccctacgg ccataactac tacccctgta actggctatg
     2041 gaaatcgctg gcgatctgct gggatggaaa ggtcgcgacc tgctgcggcg acttcggccc
     2101 ggccgacgtc ttcggtgatc tgaacacgga caccctcaga gggatctgga ac
//
LOCUS       DATALS010000545         1579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_133256, whole genome shotgun sequence.
ACCESSION   DATALS010000545 DATALS010000000
VERSION     DATALS010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133256"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..1428)
                     /gene="cobT"
                     /locus_tag="VHS06_12145"
     CDS             complement(370..1428)
                     /gene="cobT"
                     /locus_tag="VHS06_12145"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX2988904.1"
                     /translation="MISIEQAVASIKPLDRAAMDAARQRQDQLTKPLGSLGRLEELAI
                     QIAGITGQVCPRLPRKTVVVMAADHGVVSQGVSAYPADVTPQMVMNFLKGGAAINVLS
                     RQAGARVVVVDVGVASDIAPADGLLIRKVARGTRNLAAEPAMTVEEMRRAIQAGIDVV
                     EAELAKGLDLVATGDMGIGNTTPSSAIVAAIAGLPVAQVVGRGTGLDNAGLSRKIETI
                     EQGIRVNRPDPNDPLDVLAKVGGLEIAGLVGVILGGAAHRLPVVVDGFISTAAALVAA
                     KAWPSLRPYLIPSHQSVEIGHQAMLKALDLKALFDFDMRLGEGTGAAIAMQMIDDALA
                     IQAEMATFEEAGVSNKEQ"
BASE COUNT          271 a          520 c          463 g          325 t
ORIGIN      
        1 cccttgccgt ttcctgtgaa caccatcacg agccccttgg tgcgggcctt gggcttatcc
       61 tctgtcgtca tctgttcctc ccgctacgag ttacgagtgc cgggtgctga gaaggattgt
      121 atcacccgac ctgtgccgga gcgcgggcgt cttgcccgca gcgagcaaac gaacaggatg
      181 gacaggatga gacggatata caggatgagg acgaatcatt tcgattctca tcctgcatat
      241 cctgtgtatc ctgtttgaat cggtcccccc acgcgttgcc acatcacgct gatgcgggcg
      301 aggacgcccg cgctcccagg atttggcctg ctggtacaat accgttcagc actcagcact
      361 cagcaccggc tattgctcct tgttgctgac tccggcttcc tcgaacgtgg ccatctccgc
      421 ttgaatggcc agcgcgtcgt cgatcatctg cattgcaata gccgctccag ttccctctcc
      481 cagccgcatg tcgaagtcga agagcgcctt cagatccagt gctttcagca ttgcctggtg
      541 gccgatctca accgactgat gggatgggat caggtatgga cgcagcgatg gccaggcctt
      601 ggcagctacc agcgcggccg ccgtagagat gaagccgtcg accactaccg gcagtcgatg
      661 ggcggcgcct cccagaatca cccccacgag cccggcaatc tccagcccac ccacctttgc
      721 cagcacgtcc agcgggtcgt tggggtctgg ccggttgacc cggatcccct gctcgatggt
      781 ttcaatcttc ctcgacaatc cggcgttgtc taacccggtg cctcgtccca ccacttgggc
      841 gacggggagg ccggcgattg cggccactat ggctgaggaa ggggtggtgt tgccgatgcc
      901 catgtcgccg gtggcgacca ggtccagccc cttggccagc tcagcctcca cgacatcgat
      961 ccctgcctgg atggccctgc gcatctcctc cacagtcatg gcaggctccg ccgccaggtt
     1021 tcgggtgcct cgcgccactt tgcggatcaa cagcccgtcg gcaggggcga tatcggaggc
     1081 gacgcccacg tccacgacga ctaccctggc gcctgcctgc ctggacagaa cgttgatggc
     1141 ggccccgccc ttcagaaagt tcatcaccat ctggggtgtg acgtcggctg ggtaggcgga
     1201 gactccctgc gagacgaccc cgtggtcggc agccatcacc accacggtct ttcgagggag
     1261 tcgagggcag acctggccgg tgatgccggc gatctggatc gccagctctt ccagcctgcc
     1321 gagactcccc aacggcttgg tcaactggtc ttggcgctgt cgagcagcat ccatggcggc
     1381 tcgatccagc ggctttatgg atgcaacggc ttgttcgatg ctaatcatta cttcctccct
     1441 aataggtact ccggggaact cttggaactc tgaggaactg agaaggccat tcgctccatt
     1501 ctctaccacg ttgctcttgc gccaagtagg gcgggtcgcc cccgacccgc ccggggcgtg
     1561 gttacggcag gatacgggc
//
LOCUS       DATALS010000546         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_81620,
            whole genome shotgun sequence.
ACCESSION   DATALS010000546 DATALS010000000
VERSION     DATALS010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81620"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..477)
                     /locus_tag="VHS06_12150"
     CDS             complement(91..477)
                     /locus_tag="VHS06_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX2988905.1"
                     /translation="MPRFGLVPQTQRFFELFEEDAKNLLAGAKLMRETLDKFEQAPRL
                     VKKLESLEHEGDRITHDLFAELNKTFVTPLDREDIHSLAVRWGVAGDMLMAWVLTIPA
                     TATVSWLLFECFAVTRAALAMVGVGA"
     gene            complement(560..>1574)
                     /locus_tag="VHS06_12155"
     CDS             complement(560..>1574)
                     /locus_tag="VHS06_12155"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX2988906.1"
                     /translation="TGDLRLVEELREPMHMALEWVRRHGDINGDGFLEYQSRSSRGLR
                     NQGWKDSANAVCWRDGEVVETPIAPVEAQGYGYDARVRVAELLAATGEGDLAAELLAE
                     AAVLKEQFERSFWLQEERFLAQALDSCGKAVPVVTSNPGHCLWSGILSTEKADGVVRR
                     LMAGDMDSGWGIRTMSSLEPGFNPMSYHNGSVWPHDNSLIVAGLKRYGYRREAERVFT
                     EVYEAASRFGGYRIPELYCGFDRDRHYQSMPAQYPVSCSPQAWSAGSVFLMLQQMLGL
                     RPDAVNSVLYVSPSLPAWLKRLSLRRLRVGDGSVDLTFVSDGKKVHVDVHRAENLKVI
                     QEP"
BASE COUNT          311 a          562 c          428 g          273 t
ORIGIN      
        1 agagggtggc gaagtaaggg gataggagga cctctcaccc tgcccctccc cgctgcgggg
       61 aggggaaagg ggtggggtta gagccgagcc ttacgctccc accccgacca tcgccagtgc
      121 agcccgagtg acggcgaagc actcgaaaag cagccatgac accgtcgccg tggccgggat
      181 cgtcagcacc caggccatga gcatgtcccc ggcgacaccc catcgcaccg ccagggagtg
      241 gatatcctcg cgatccaacg gagtgacgaa ggtcttgttc agctccgcaa acaggtcgtg
      301 ggttatccga tccccctcgt gctccagact ctccagcttc ttgaccagac gaggggcctg
      361 ctcgaacttg tcgagtgtct ccctcatcag cttcgcccca gccagcaggt tcttggcatc
      421 ttcttcgaac agttcgaaga accgctgggt ctgaggcaca aggccaaatc taggcactct
      481 ggtctccttt cgcattggcc ccgactgcgg ggtactgagt gatgagtagg acccagaact
      541 cagaactcag aaccggtggt cagggctcct gtatcacctt caagttttct gctcgatgca
      601 cgtcgacgtg caccttcttg ccatcgctga caaaggtcag atctaccgag ccatcgccca
      661 ccctcaaccg gcgaagcgac aaccgcttca accaggccgg aaggctcgga cttacgtaaa
      721 gcaccgaatt cacggcgtcg gggcgcaacc ccagcatctg ctgcagcatc aggaacaccg
      781 acccagcgga ccaggcttga gggctgcagg acacgggata ctgggccggc atcgactggt
      841 aatggcgatc ccgatcgaac ccgcagtaca gctctgggat ccggtaaccg ccgaacctgc
      901 tggcggcctc gtacacctcg gtaaacaccc gctccgcctc ccgccggtaa ccgtacctct
      961 tcagtccggc aacgatcagc gagttgtcgt gaggccacac cgagccgtta tggtagctca
     1021 tcgggttgaa acccggctcc agggaactca tggtccggat gccccagccc gagtccatat
     1081 cacccgccat caaccgccga accaccccgt cggccttctc cgtggagagg atgccggacc
     1141 agagacagtg gcccgggttt gatgttacca cgggaaccgc tttaccgcac gaatccagcg
     1201 cctgggcaag aaaccgctcc tcctgcagcc agaaactccg ctcgaactgc tcctttagga
     1261 ctgccgcctc tgccagaagc tccgccgcca gatccccctc gcccgtggcc gccagcagct
     1321 ccgccacccg cacccgagca tcgtagccgt agccctgcgc ctccaccggc gctatgggtg
     1381 tctccaccac ctcgccgtcc cgccagcaga ccgcgttggc cgaatccttc cacccctgat
     1441 tcctgaggcc gcggctggac ctgctctggt actcgagaaa cccatccccg ttgatgtccc
     1501 cgtgacgccg aacccactcc agggccatgt gcatcggctc ccgaagctct tccaccagcc
     1561 tcaagtctcc ggtc
//
LOCUS       DATALS010000547         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_133563, whole genome shotgun sequence.
ACCESSION   DATALS010000547 DATALS010000000
VERSION     DATALS010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133563"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..320)
                     /locus_tag="VHS06_12160"
     CDS             complement(90..320)
                     /locus_tag="VHS06_12160"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2988907.1"
                     /translation="MPPTDRATTGVHWLDTRSLADYLNVTTRAVESWRQLGDGPPFVK
                     VGRLCRYRRADVDAWIESRVRRSTSSTGPKSE"
     gene            complement(517..1773)
                     /locus_tag="VHS06_12165"
     CDS             complement(517..1773)
                     /locus_tag="VHS06_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX2988908.1"
                     /translation="MPVGLTKRQIEAAKVAAHEYTLWDDNPPAFGVRIRPGGSRTFIL
                     KYRVDGRQRKLTLGSYGALTVEQARKLAKTALASAAAGTDPAQAKLEARRAPTMKDLC
                     DRFESEYIAVRLKPKTARSYRRLMETRIAKHFGPMKIGAVTRADVAKWHQSLKATPVE
                     ANRALLLLHRLFVMAGVWGLYEGANPANAVEKYGEKARKRYLQGNELYRISNAISELE
                     AAPKRPLSPFVGLAFRLLIATGCRRDEVLTLKWEDVDLDAMVLRLKDSKTGEKTVVLS
                     SFAVDLLKHADTQDGSPWVCTRKRRNSKREWSHIVNPASAWQDVKERASAHVEGEPDV
                     DVMDVTLHDLRHAYASVGVSMGLSLPIVGALLGHADQASTQRYSHLQVDPLRLAAERI
                     GGEIAAAMAGTPPAQVVNMEEARQAR"
     gene            complement(1937..2008)
                     /locus_tag="VHS06_12170"
     tRNA            complement(1937..2008)
                     /locus_tag="VHS06_12170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1973..1975),aa:Arg,seq:cct)
BASE COUNT          382 a          684 c          625 g          445 t
ORIGIN      
        1 cgggcaaccc gaggccgagc atctgtgcca gcctacggac cttccgatgg gtgggcgtgg
       61 actgttctat cgggaggtac accggtccct cattccgact ttggcccggt ggagctggtc
      121 gagcggcgca cccgactttc aatccatgcg tcaacatcgg ctcggcggta ccggcagagt
      181 cggccaacct tcacaaaggg tgggccgtca ccaagttgcc gccacgattc gaccgcacgg
      241 gtcgtgacgt tcagataatc ggcaagagat cgggtgtcaa gccaatggac accagttgtc
      301 gcgcggtcgg tcggcggcat atcgcttcct ctccttcctc gaagccacta cttacgccga
      361 ccccgccaaa caaaacggag gccggactct acgtctcgac ctccgtttga ttcacttccg
      421 ctgccccggc tcctcacagc ctagcagcgc tacacacaca ctagcacata ccgctgtccg
      481 cgtacagccg ccgctgtggg cggaatcgcc gggtatctaa cgcgcctgcc gcgcctcctc
      541 catgttgacc acctgagcgg ggggagttcc ggccatggcc gccgcgattt cgcctccaat
      601 gcgttcggcc gcgagccgca gggggtcaac ttgaaggtgc gaataccgct gtgtgctggc
      661 ttggtccgcg tgcccgagga gagcgccgac aatgggtaag ctgagaccca tgctgactcc
      721 gacgctcgcg taggcgtggc gaagatcatg cagggtaacg tccatcacgt ccacgtccgg
      781 ttccccctcg acatgagcgc ttgcccgctc tttcacgtcc tgccaggcgc tcgcggggtt
      841 gactatgtga ctccactccc gcttgctgtt gcgccgcttc cgcgtacaaa cccaaggaga
      901 gccgtcctgt gtatcggcgt gcttgagaag atcaacggca aaggaggaaa gcaccacggt
      961 cttctcgccc gtcttgctgt ccttgagccg aagcaccata gcgtctaggt caacatcttc
     1021 ccacttgaga gtaagcacct cgtcccggcg acagccggtg gcaatgagca gccgaaaggc
     1081 aagccccacg aaaggagata gcggcctctt gggtgcggct tcgagttccg atatggcgtt
     1141 gcttatccgg tagagttcat tcccttgcag gtaccgcttg cgggcctttt ccccgtattt
     1201 ctcgacagca ttagccgggt tcgcaccctc gtaaaggccc cagaccccgg ccatcacaaa
     1261 gagtctgtgc aacagcagca gcgcccggtt cgcctcgacc ggcgtggctt tcaaagactg
     1321 gtgccacttg gctacatctg cccttgttac cgcgccgatc ttcattggcc caaagtgctt
     1381 agctatccgc gtctccatca gccgccgata ggaacgcgcg gtcttgggct tcagacgaac
     1441 agcgatgtac tcgctctcaa aacggtcgca caggtccttc atcgtaggcg ctcgccgggc
     1501 ttccagtttc gcctgtgcag ggtccgtgcc agcagccgca ctcgctaggg cggtctttgc
     1561 cagtttgcgc gcttgctcga ctgtcagagc gccgtagctg ccaagagtca gcttccgttg
     1621 tctgccgtct acccggtatt tcaaaatgaa ggtccggctg ccgccgggcc ggatgcgcac
     1681 cccgaaggcg ggcgggttgt catcccaaag cgtgtactcg tgcgcagcga ccttggccgc
     1741 ttcaatctgc cgcttcgtaa ggcccactgg caccccctcg tagccgggtt ctgtgtgctg
     1801 ggtaccagaa tcgggctgtt tccctaggct gggtaccaca tgggtaccag tctaggtgat
     1861 atttggtgct agtgcaagat aggtgattcg ggggaggaca ggcccattta cgggccttta
     1921 tgctggaatt tgttagtgcc cccggtagga ctcgaaccta cgcacgaggt ttaggaaacc
     1981 tctgctctat cccctgagct acgggggcgc cctcagcctg tggcggcagg gccacagccg
     2041 gcagattcta gcattctggt cggagaccac cttgatcttc cagggctgca ggttgatagc
     2101 cgataccgcg tagctgacga tctcgaggag ctcccg
//
LOCUS       DATALS010000548         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_134140, whole genome shotgun sequence.
ACCESSION   DATALS010000548 DATALS010000000
VERSION     DATALS010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134140"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..>1803
                     /locus_tag="VHS06_12175"
     CDS             141..>1803
                     /locus_tag="VHS06_12175"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX2988909.1"
                     /translation="MTEYYETDLAGRRLVIETGRVAQLADGAALVRYGDTIVLGTAVS
                     SDQPREGVDFFPLTVDYEEKMYAAGKIPGGFIKRESRPSEQAILAARLTDRPIRPLFP
                     KAYRNDVQVILTVLSADQLNDPAIVGTIAGSAALSMSDIPFFGPVAAVKVGYIDGEFV
                     LNPTMPQLANSRLDLTVSGTADAVMMVEAGAQELPEDVVLEAIQFAHRGMQPIIEVQK
                     RMMAAIGRPKREYAEVEIPDETRAAVAALIKDRLAAAVNNPDKAAREESTGLLKRETL
                     EALQESHDPILVAKVFDEMHKAEVRTAILQKGTRPDGRILNQIRQLSAEVGLLPRTHG
                     SGLFTRGQTQVLSVATLGSVSEEQIIDGIGIDVSKRYMHHYNFPPYSTGEVRPLRGAS
                     RRDIGHGHLAERALAAVIPSAEQFPYTIRVVSEVVSSNGSTSMASVCGSTLALMDAGV
                     PIKSPVAGIAMGLVIGEGKFAILTDIQGVEDALGDMDFKVAGTSEGITALQMDIKVKG
                     ITPQIMEQALAQAKAARSTVLGVMLDCIQEHRAELSPYAPRLVKIQ"
BASE COUNT          363 a          530 c          559 g          351 t
ORIGIN      
        1 gcacctagcc tacctcagta agaaggacaa cgccaagtac caggtgctta tcgcgcagct
       61 cggtctacgc aagtagtcag gcaggcacct tgcttgcaca ggtaagggga taggtctggg
      121 ctcgaccacg aggagaacga atgacggaat actatgagac cgacctagcc ggcaggcggt
      181 tggtcattga gactgggaga gtcgctcagc tcgcagatgg cgcggctctg gtccggtacg
      241 gtgataccat cgtgctgggt actgcggtta gttctgatca accccgggag ggggttgatt
      301 tctttcccct cactgtcgac tacgaagaga agatgtatgc cgccgggaaa atccccggcg
      361 gttttatcaa gcgtgagagc cgtcccagcg aacaggctat cctggctgct cgtctgaccg
      421 accgtccgat ccgccccctc tttcctaagg cttaccggaa cgacgttcag gtgatcctga
      481 cggtgctctc tgccgaccaa ctgaacgatc cggctattgt tggaacgatc gcggggtcag
      541 ccgccctctc catgtcggat attcccttct tcggtcctgt ggcggcggtc aaggtcggtt
      601 acatcgacgg cgagttcgtg ctcaacccca ccatgccgca gttggccaac agcaggcttg
      661 acctgactgt gtccggcacg gcggacgcgg tgatgatggt tgaggccggt gcgcaggaac
      721 ttcccgagga cgtcgtcctc gaggcgatcc agttcgctca ccgtggcatg cagccgatca
      781 tcgaagtcca gaagcggatg atggcagcca tcgggcgacc caagagggag tacgccgagg
      841 tggagatccc agatgagact cgcgcagcag tggctgctct catcaaggat cgactggctg
      901 cagccgtgaa caatccagat aaggctgccc gcgaggagtc caccggtctg ctgaagcgag
      961 aaacgctcga ggcactccag gagagccacg atcccatcct cgttgccaag gtgttcgatg
     1021 agatgcacaa ggccgaggtg cggaccgcca ttctgcagaa gggcacccgc cccgacggca
     1081 gaatcctcaa tcagatcaga cagctgagtg ccgaggttgg attgttgcca aggacccacg
     1141 ggtccggcct tttcacccga ggccagacac aggttctttc tgtcgccaca ctcggctctg
     1201 tcagcgaaga gcagatcatc gacggcatcg gcatcgacgt ctcgaagcgc tacatgcacc
     1261 actacaactt cccgccctac tccactggag aggttcggcc attgcgtggt gcgtcccgcc
     1321 gggatatcgg acacggccac ctcgcggaga gggcgctggc ggcggtaatc ccttcggcag
     1381 agcagttccc gtacacaatc cgagtcgtgt ctgaggtggt gtcttccaac gggtctacct
     1441 ccatggccag cgtttgtggc agcactcttg ccctgatgga tgccggcgtg ccaatcaagt
     1501 cgcctgtggc cgggatcgcg atgggactgg tgatcggtga gggcaagttc gcaattctca
     1561 ccgacatcca gggagtcgag gatgcgctcg gcgacatgga cttcaaggtt gctgggacgt
     1621 ccgagggtat caccgccctt cagatggaca tcaaggtgaa ggggatcact ccccagatca
     1681 tggagcaggc gttggctcag gcgaaggctg ccaggtcgac ggttctcggt gtcatgctgg
     1741 actgcatcca ggagcatcgt gccgagttgt cgccctatgc gccacgcctg gttaagatcc
     1801 aga
//
LOCUS       DATALS010000549         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_81927,
            whole genome shotgun sequence.
ACCESSION   DATALS010000549 DATALS010000000
VERSION     DATALS010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81927"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VHS06_12180"
     CDS             <1..331
                     /locus_tag="VHS06_12180"
                     /inference="COORDINATES: protein motif:HMM:NF025638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEX2988910.1"
                     /translation="QVKSTTNAIVRGGEIAYDLDRDAYEDLRSMEVSTPRILVLHVQP
                     KAEPERLTVTQRGLLVRGRCYWTSLRGLPGVANLRQVRIRIPMSHALTVDSLREILRR
                     VSEEKQF"
     gene            331..1098
                     /locus_tag="VHS06_12185"
     CDS             331..1098
                     /locus_tag="VHS06_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988911.1"
                     /translation="MGRFTLRDLRQPISLRAARDYLAQHGWRIREDGQRLVCEGPADD
                     ESRPIVQFLPADESYSDYPLRLEDLISALSVLEERPAVEIAAEMARYDESPPSPAGLL
                     AEELIAELGRSGIQLAPGKDLQQVVNELRPLMASTELAIQQGPLHDLATRQQAALLAV
                     RFVRLVAVNRASQMLLWWLCDRLLSAGGFRLSLLPEQVEELCRVASLDDALAPDEVLD
                     WITRHARKVRSKKVEMKGPVAGAKRPDPPADSGGPVH"
     gene            complement(1078..1383)
                     /locus_tag="VHS06_12190"
     CDS             complement(1078..1383)
                     /locus_tag="VHS06_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988912.1"
                     /translation="MAADLRTLYEAYVDYVARLRDDFDRHVGAGVPERYRPRLLNLEE
                     FCDAWRRWGHVGSFQETWQRRFALGYDHVADALSKELGVALATAGHGCEGRLVDRAA"
     gene            complement(1532..>2111)
                     /locus_tag="VHS06_12195"
     CDS             complement(1532..>2111)
                     /locus_tag="VHS06_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX2988913.1"
                     /translation="KGCERCIRWAFDYTRKRNKKKQLTLVAKTNVLTYGHNLWERTFY
                     EVAKEYPDIKTDYNHVDACCMWMVKNPEFYDVIVTTNMFGDIITDLAGILQGGMGVAA
                     GGNINPDKGGLSMYEPMGGSAPKYTGKHVINPIATINAASMLLEHSGYPKAGARVMKA
                     IQCVTGTKMKSQAAGKMGYSTQEVGDLVCEAL"
BASE COUNT          403 a          636 c          647 g          425 t
ORIGIN      
        1 ccaggttaag agcaccacca atgcaatcgt ccgaggtggt gagatcgcgt atgatttgga
       61 tagggatgcg tacgaggacc tccgcagcat ggaggtttcc acgcctcgga ttctcgtctt
      121 gcacgtccaa ccgaaggccg agccggaacg ccttacagtg actcagagag ggcttcttgt
      181 acgaggacgc tgttactgga cgtccctgag ggggcttccg ggagtagcga acttgaggca
      241 ggtccggatt cggattccca tgagccatgc actcacagtg gatagtctga gggagatcct
      301 aaggcgcgtc agcgaggaga agcagttctg atgggacgct ttacattgag agacctgaga
      361 caaccgatct ctctccgcgc agcgcgggat tacctggctc agcacggatg gcgtattcgc
      421 gaagacggtc agcgcttggt ctgcgaaggg cccgccgacg acgaaagtcg gccgatcgtc
      481 caattcctgc ctgcggacga gtcgtattcc gactacccct tacggttgga ggatctgatc
      541 tcggcgttga gcgtgcttga ggagcggccg gcagttgaaa tcgccgcgga gatggctcgc
      601 tacgacgaat cgccaccttc gcctgcgggc ttgctggccg aggaactcat cgctgaattg
      661 ggccgcagcg gaatccaatt ggcgcccggc aaggaccttc agcaggtggt caacgaactg
      721 cgccctctta tggccagcac cgaattggca attcaacagg gcccgctaca cgacttggcg
      781 accaggcaac aagctgcgtt gttggccgtg cgtttcgtcc ggctcgtggc cgtcaaccgc
      841 gcatcgcaga tgcttctttg gtggctctgt gaccgcctgc tctcggccgg gggtttcagg
      901 ctctcattgc tccccgagca ggtggaggaa ctatgcaggg tggctagctt ggatgatgca
      961 ctcgctccag acgaagtcct cgactggatc acacgccatg cgagaaaggt ccgcagcaag
     1021 aaggtcgaaa tgaaaggccc ggttgcaggg gcgaaacgac ccgacccacc ggccgattca
     1081 ggcggccctg tccactaacc tgccttcgca gccatggcct gcggttgcga gtgcgacccc
     1141 caactccttc gacaaggcgt cggccacatg gtcatagccg agagcgaagc ggcgctgcca
     1201 cgtctcctga aacgatccaa cgtggcccca acggcgccac gcatcgcaga actcttccaa
     1261 gttcagcaat cgggggcgat accgttcggg aacgcccgcg ccgacgtggc ggtcgaaatc
     1321 gtcgcgcaga cgcgcgacgt agtccacgta cgcctcgtac aacgtccgta gatcggcagc
     1381 catgaaccag gcctccattc gctgattccc gacggatggg ataggctccc gcgcatcgct
     1441 cgagccctga ttgtacccat ccgaccggga acgcaaaggc cggttttgtt aaaacaggca
     1501 aactggatat gtaggaataa ttcccaggag ttcaaagcgc ttcgcagacc aaatcgccga
     1561 cttcctgcgt gctgtaaccc atcttgccgg cggcctggct cttcatcttg gtgccggtga
     1621 cgcactggat ggccttcatc acacgagccc cggccttggg ataaccgctg tgctcgagga
     1681 gcatcgaggc ggcgttgatc gtggcgatgg ggttgatcac gtgcttgccg gtgtacttcg
     1741 gggcgcttcc gcccatcggc tcgtacatgc tcaggccgcc cttgtcgggg ttgatgttcc
     1801 cgcccgccgc cacgcccatc ccaccctgca gaatgccggc caggtcggtg atgatgtcgc
     1861 cgaacatgtt cgtggtcacg atcacgtcgt agaactcggg gttcttgacc atccacatgc
     1921 agcaggcgtc gacgtggttg tagtcggtct tgatgtccgg gtattccttg gccacttcgt
     1981 agaacgtacg ctcccacagg ttatggccgt aggtcagcac gttggtcttg gcgaccagcg
     2041 tgagctgctt cttcttgttc cgcttgcgcg tgtagtcgaa ggcccagcgg atgcagcgct
     2101 cgcagccctt g
//
LOCUS       DATALS010000550        11477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_81943,
            whole genome shotgun sequence.
ACCESSION   DATALS010000550 DATALS010000000
VERSION     DATALS010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11477
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81943"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..546)
                     /locus_tag="VHS06_12200"
                     /pseudo
     CDS             complement(94..546)
                     /locus_tag="VHS06_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447288.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1052..3481)
                     /locus_tag="VHS06_12205"
     CDS             complement(1052..3481)
                     /locus_tag="VHS06_12205"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX2988914.1"
                     /translation="MPDFILPFTSPDATLAVVGGKGTNLSRLTRAGFPVPPGFLITTA
                     AYHSFVEANDLQTEIVRLATDPRKSSEEASTAIRRLFDRGHIPPEVTVTIQHAHAELV
                     RATADKLSLAVRSSATAEDLPGASFAGQQESYLNVRGETALLDAVKRCWSSLWTARAI
                     DYRLRQGIDPSRVGLAVVVQTMVHAEVSGTLFTANAVTGARDEIVLDAAWGLGESIVG
                     GLVTPDHVVVDKATGAIRQMVIGDKAVMTVPTSTGTTEREVEAGKRQAQALSAAQVAA
                     LVKLGAAIESHYGVPQDIEWCLADGRFWIVQSRPITALPPEPVRWESPVPEAKWIKDV
                     QAGEWATEPLSPLGATTTFAAMIAARQRAFPMQRYPWSALINGWIYVRADFRVPQMMV
                     WLMGVVVRFAAGTLDGHQRVRRTWPHQLTILDSLEGTELARLSDDELRAHANRLLDAL
                     GWWWWEISWDAAAALIGEQLIGKLRVPNLADPAVLFRGNDSLLLEAERALRLAADTGE
                     VESYLARFGHFVESADPIHPTLRESPDLLAQHLAVAHRSETTPDERLMRTRRERTEAE
                     SLVRAQRGTRGFLARRLLTVGQSFAAHVDDAVFHFQRVLALVRATFLEVGRRLARAGA
                     VERAEDVFYLEYGEVWAPPADLAERVTQRRLLREHHRRLAPPPFIPPLSDPIWERDPL
                     MNLLSSALGTAVLRRGVQERNGGRILVGTPGSPGRARGTARVIAGPDDFHRFQPGDVL
                     VAHTTTPIWTPLLNVASAAVTEVGGPFSHAAIVAREFGIPLVNGALDATQVIADGMSI
                     VVDGSAGIVEL"
     gene            complement(3474..3638)
                     /locus_tag="VHS06_12210"
     CDS             complement(3474..3638)
                     /locus_tag="VHS06_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988915.1"
                     /translation="MDIRILPRLMSKLREMRKHERWTRPQWATVMATFGRAHEWAGVP
                     TTPMQGHQHA"
     gene            complement(3904..4563)
                     /locus_tag="VHS06_12215"
     CDS             complement(3904..4563)
                     /locus_tag="VHS06_12215"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017307544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX2988916.1"
                     /translation="MDTGPSDYGLWPLVAINAAVFIIFAFSFVRPRTSRDWRSFGAFS
                     AFLVALFTEMYGFPLTLYMLSGWIGSRYPGLDLLAHNNGHLWETLLGWGGDPHENPIH
                     LLSDLLIAGGFLLLAAAWKVLYAAQKEKGLATSGIYSRVRHPQYIGFITIMLGFLIQW
                     PTFLTLAMFPILVPMYVKLARREEREVAAEFGDEYARYAAATPAFIPRLSLKPAESRT
                     M"
     gene            complement(4616..4993)
                     /locus_tag="VHS06_12220"
     CDS             complement(4616..4993)
                     /locus_tag="VHS06_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2988917.1"
                     /translation="MTADKIIVILGGLVTIVGIAWFFWGPRKGGFRAAVTSSGYQEAM
                     VLVKGGYTPDVIVVQHGKPVRLSFRREESAACSEMVVFGDFNKSATLPEGEIVPIEFM
                     PERPGEYDFTCQMGMFRGKLIVE"
     gene            complement(4993..7542)
                     /locus_tag="VHS06_12225"
     CDS             complement(4993..7542)
                     /locus_tag="VHS06_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2988918.1"
                     /translation="MVTMDTKRRSTENGAPSATTGVAERVFGPVSIDLPVARLTRSGG
                     PALEQALQTAPGVEKVRVDVEGKRVRVDYDPTLIGVGQLVEAVRQAGYDTGTASIRLK
                     VSGLYCAECASVIEDELKATPGVLDATLSAATEEVKVDYLPSSVNLESLTHAVENAGP
                     YKAAPAAPASEPGLDKESSEQGQEYRQLMRKWWFGAAVAIPTMALSYPFLFPGLRDVL
                     VRGSNELRIVWGLMALASLAVMVYSGNQFFTGMWQGLMHRSANMHTLIAVGTGVAWLY
                     STIALLFPQVFPSAEFVDVYYDVTVVVTSLVVLGLAMEVKAKGRTSDAIKKLIGLQAK
                     TARVIRDGREVDIPVEEVLVGDVVLVRPGEKIPVDGVVLEGQSAVDESMITGESIPVA
                     KGVDDEVIGATLNATGSFKFRATRVGKDTALANIVRMVQDAQGSKVPIQRIVDVVSGY
                     FTPAVMILAVFGFVVWYVFGPPPHLAFALIVAVTTLIIACPCALGMATPMSLTTGVGL
                     GAQNGILIRSGEALQTASRLQTLILDKTGTITKGKPELTNVVADNGFQEEDILRISAA
                     VERNSEHPLATAIVDGAKVQGLALPEISDFNAIPGQGVEARVEGRKVLLGNAKLMAGQ
                     AVALGRLELEAQRLADDGKTPMFAAIDGTAAGIVAVADTVKEDSKAAIATLKALGLEV
                     VMLTGDNPRTAKAIGRQVGIDRVLAEVLPQDKAHNVQKLQLEGKKVGMVGDGINDAPA
                     LAQSDVGFAIGTGTDVAIEAADVTLISGSLRGVVSAVDISKATMRNVYQNLLGAFVYN
                     GLGIPVALGVLYPFTGILLSPLLAALAMSFSSVTVIGNANRLKRWKAKEAR"
     gene            complement(7844..8521)
                     /locus_tag="VHS06_12230"
     CDS             complement(7844..8521)
                     /locus_tag="VHS06_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2988919.1"
                     /translation="MKRILVIDDDPAVTSVLKRGLSYEGFVVDTASGGSQGLEIARER
                     VPDLVILDIMMPGVDGLEVLRRLHAADPRLPVLFLTARDAPADQIQGLELGADDYVVK
                     PFTFGVLLARVQAALRRYEPDRPEILRFEDLSLDAGTRLARRGDREIELTKTEYDLLY
                     EFLRQPRRVLTREILMDHVWGYDFEGNTNILEVYVRQLRQKLEAAGEPRMIHTARGVG
                     YALREQE"
     gene            complement(8753..8902)
                     /locus_tag="VHS06_12235"
     CDS             complement(8753..8902)
                     /locus_tag="VHS06_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX2988920.1"
                     /translation="MAKDPVCGMNVDEKKAAAKSEYQGQTYYFCSPGCKAAFDKEPQK
                     YAGKK"
     gene            complement(8964..9212)
                     /locus_tag="VHS06_12240"
     CDS             complement(8964..9212)
                     /locus_tag="VHS06_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988921.1"
                     /translation="MIEFIQANASWIVLAALFVLMMRMHGSGGGMGCCGGHQHGQQDR
                     EPDQSQRSLPGPRQEEKGEEKEKEPVASGSRGHSGGCH"
     gene            complement(9234..10508)
                     /locus_tag="VHS06_12245"
     CDS             complement(9234..10508)
                     /locus_tag="VHS06_12245"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2988922.1"
                     /translation="MDNRNLADNNQEPRLTPQLGVGQTMAVALGAMLGAGVYVSMGQA
                     AGTTGGSLLVAVVLGAVVATINGLSSAELGAYDPRAGGAYQFARNLVTPAVGFVAGWL
                     FLFAALTAGGTYALTFGAYLEALLPGVPLRAVGVALVAVAVISNMLGVRISGRVSVAL
                     VVINVAILLAFVGLALPAFTPHNLRPFVLGGARGFLQASALLFFAYTGYARPVTVAEE
                     VRDPAWTLPRAVPTAVGIVMLLYFGVAFAALGVLGPERLGEEAAPLRAAMVAAGSPAG
                     ATLLAVGALLASFAVLLTEIWGLSRLAFAMGRNGDLPACFGRLSGPGLIPRNAVLAAG
                     SVLVLLAAFADLRPLLEASSLALLVYYWVMNLSALRLPPERRLYPLVVPVAGIVANPV
                     VGLSLPLQTLVVVGAMAVTGLAYFALLQRLRK"
     gene            complement(10636..11307)
                     /locus_tag="VHS06_12250"
     CDS             complement(10636..11307)
                     /locus_tag="VHS06_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2988923.1"
                     /translation="MVGVLATGLLVIVLAKGPTWRRDPNRLFRAMYNLVAPIYDLWAG
                     EQSALTPLRRQLAEMMELEPGDQVLEVGAGTGANFPFIAAKVGSSGHIYAQDLSEGML
                     AQARKKVATMPCPVDLQQGQAEDLPYPDNSFDAVLHLGGINFFTDPRRALEEMSRVAK
                     PGAKIVVSDETAPPGTGFGGLVSGAILRLIPRLRPPMHLIPDPNPQLHYLAGGYIYAI
                     DWRKP"
BASE COUNT         2165 a         3807 c         3453 g         2052 t
ORIGIN      
        1 gcgagccttg cgcctcgccg ccgacacggg cgaggtcgag agctacctgg cacggttcgg
       61 ccacttcgtg gaaagtgccg atcccatcca cccaacactt cgcgaggccg gatcgaactg
      121 ggtgttgcct gcctctccgc ccagggggat cgtcgccacc cgccggttcg tgccggtatc
      181 gatgacggtg tcggtgcccc cactctcgtc ggacacgtag agcttgccgt tgtccggatc
      241 gtaggcgagt ccgtcgggat agttgccggt cggaactgtg gcgacgacgg aaaggctctg
      301 ggcgtcgatg acggctacct cgttgtcgcc cgtggccgtc gcatagaccc tgccgagctc
      361 cggcaccgcc aggaccccat ggacgtcctt cacgccggtc acgttctcca ctaccttccc
      421 tgcctgagta tcgaagactg taatgagact ggcacccagg tgtgcaatgt acagccgatg
      481 ggactgcgga tcgaggctct ggtagtcgaa tcgtgatcgg cctccaggaa gcgggacgtc
      541 ggccacggta cgaaagccgt actggctggt cgggcttgtt gcagaagccc cggtgtcgga
      601 tgtcgaagga gcaaccccag gacctaccgc cgaggatccc aacgagggtt gggtggtggg
      661 agcagatgtc gaatccacgc cacatgagag ggcaagcact gaaccgacga gcgcgaacga
      721 agccgccgca accgggatgc gcccaaacca gagcaagccg accagcccgc cggtgaagca
      781 cattgtccag cacctctatg cgatgcgcga atgatcgccg cctaactgca tcctgttccg
      841 caggaaccgc gtcagcgcat tcggtgggcc agagtctgct tcagagccca cgtagctact
      901 attgagatta tctggacgat ggcttccgct atccgacgga aaggctccgg tgatcatcgg
      961 caggtgggtt ctgcctaccg tgggcagact agtctcatcc accagagcgc ataaggaggg
     1021 ggtaacagaa tcgggtcacg catcgatcag ttcacagctc aacgatgcct gccgagccat
     1081 caaccacgat ggacatcccg tccgcgatga cctgcgttgc atcgagtgct ccattcacca
     1141 gtgggatacc gaactcccga gccacgattg ccgcgtgaga aaacggaccc ccaacctcgg
     1201 tcaccgcagc actcgcgaca ttcaagaggg gcgtccatat cggcgtcgtg gtatgggcaa
     1261 cgagcacatc ccccggctgg aagcggtgga agtcgtccgg cccagcgata acccgtgcgg
     1321 tgccacgtgc acgaccggga cttcccggtg tgcccaccag tatccgcccc ccgttgcgtt
     1381 cttgcacgcc gcgcctgagt actgctgtac ccagtgccga tgacaggagg ttcatcagcg
     1441 gatcacgctc ccagatggga tcggagaggg gtgggatgaa cggaggcggc gcgagccgcc
     1501 tgtggtgctc tcggagcagg cggcgctgtg tcacgcgttc cgcaagatcg gccggaggcg
     1561 cccaaacttc gccgtattcc aggtagaaga cgtcctctgc ccgctccacg gctcccgcgc
     1621 gcgccagccg ccgaccgacc tcgagaaacg tcgcacgtac gagcgccagg acgcgctgga
     1681 agtggaatac cgcgtcgtcg acgtgggcgg cgaagctctg cccgacggtg agcaaccggc
     1741 gcgcaaggaa gcctcgagtt ccgcgctgtg cgcgaacgag gctctctgcc tctgtccgct
     1801 cacggcgagt acgcatcaat cgttcatcgg gggtagtctc actcctatgt gccaccgcaa
     1861 ggtgttgggc cagcaggtcc ggcgactcgc gaagtgttgg gtggatggga tcggcacttt
     1921 ccacgaagtg gccgaaccgt gccaggtagc tctcgacctc gcccgtgtcg gcggcgaggc
     1981 gcaaggctcg ctcggcctcg agcaataggc tgtcgttgcc acgaaacagc accgcggggt
     2041 cggccagatt gggaactcgc agtttcccga taagctgctc tccaatgagc gctgccgccg
     2101 cgtcccacga aatctcccac caccaccatc ccaacgcatc gagcagtcgg ttggcatggg
     2161 cccgcaactc gtcatccgaa agcctcgcca gttcggtccc ctccagggaa tccaagatcg
     2221 tcaactggtg gggccaggtg cgtcgtactc gttggtgccc atcgagtgta cctgccgcaa
     2281 agcgcacgac cactcccatg agccaaacca tcatctgggg aaccctgaag tcggcgcgca
     2341 cgtagatcca cccattgatc agtgcggacc aggggtagcg ctgcatggga aatgcgcgct
     2401 ggcgggcagc gatcatggcg gcaaaggtcg tcgtcgcccc cagtggcgaa agcggctccg
     2461 ttgcccattc gccggcttgg acgtccttta tccactttgc ctcgggcacc gggctttccc
     2521 atcgtaccgg ctcaggtggt agggcggtga ttgggcgaga ttggacgatc cagaaccgac
     2581 catcggccag gcaccactcg atgtcctgcg gcacgccata gtgcgactca atcgccgcac
     2641 ccagctttac cagcgcggca acctgagccg cgctcaacgc ctgcgcctgc cgcttgcctg
     2701 cctccacttc ccgctctgtc gtgccggtcg aggtcggtac ggtcatcaca gctttgtcgc
     2761 caatcaccat ctgcctgatc gcgccggttg ccttgtccac caccacatga tccggcgtga
     2821 ccagtccacc tacaatcgac tcgcccaagc cccatgccgc gtccagcacg atctcatctc
     2881 gcgcgcccgt caccgcgttc gccgtgaaca gtgtgcccga cacctcggca tgcaccatcg
     2941 tctgtaccac taccgccagg cctacccgcg acgggtcgat cccctgccgg aggcgatagt
     3001 cgatcgcgcg cgccgtccac aggctcgacc agcaccgctt cacggcgtcc aacaacgccg
     3061 tctcgccgcg cacgttgagg taactctcct gctgtccggc gaagctggcg cccggcaggt
     3121 cttcggcagt ggccgacgaa cgtaccgcca gcgacaactt gtcggcggtg gccctcacga
     3181 gttcagcgtg agcgtgttgg atggtcaccg tgacttcggg cgggatgtgg ccgcggtcaa
     3241 agagccgacg aatagcggtt gatgcctctt cgctcgattt ccgagggtcg gtggccagcc
     3301 tcacaatctc tgtttgcagg tcattggcct ccacaaaact gtggtaggcc gccgtggtga
     3361 tgaggaagcc cggcggtacc gggaagcccg cgcgcgtcag gcggctgagg ttggtacctt
     3421 tgccgccgac gaccgcaagc gtggcatccg gcgaagtgaa gggaagaatg aagtcaggca
     3481 tgctgatgcc cttgcatcgg ggtcgtcggc acaccggccc attcgtgcgc ccggccgaaa
     3541 gtggccatga ccgtggccca ctgcggccgt gtccagcgct cgtgtttgcg catctctctc
     3601 agcttgctca tcagtctggg cagaattcgt atgtccatga tcgtcgaatc ccgtcctcgt
     3661 cgggattggg atgtacattc ggatgcgtct ctcgcagcag acgtgccgcc acgtccggcc
     3721 cggccttcgg agcgagcgtt gcggagaaca acggagcggg gccgtgaagg ccagcccgaa
     3781 ccttcacggc cccgatgaaa aggaggatgt gatacgcgtc tatccggccg tgatgttacc
     3841 gcacgagagt taaggagaga ttaggtctgg ctgagagttg cgtgagaggc gccagatcct
     3901 ttcctacatg gtgcgagact cagccggctt gagactcagc cggggaatga acgctggagt
     3961 agctgcagcg tagcgagcat actcatcccc gaactcggcc gccacctccc gctcctccct
     4021 gcgagccaac ttgacgtaca tcgggacgag tatcgggaac atggccaggg tgaggaaggt
     4081 cggccactgg atcaggaacc cgagcatgat agtgatgaag ccgatgtact gcgggtgccg
     4141 aacgcgagaa tatatcccag acgtggccag acccttctcc ttttgggccg catagagcac
     4201 cttccatgct gcggccagca acaggaagcc cccggcaatc aacaggtcgc tgagaaggtg
     4261 aattgggttc tcgtgaggat cgcctcccca acccaagagc gtctcccaga gatggccgtt
     4321 attgtgcgcc agcaggtcga gtcccgggta acggctccca atccacccgg atagcatgta
     4381 cagggtgagc gggaaaccgt acatttcggt aaacagcgcc accaggaagg cggaaaacgc
     4441 cccaaaggag cgccagtcgc gggatgtgcg cggtcgcacg aagctgaaag cgaatatgat
     4501 gaacacagcg gcgttgatcg ccaccaacgg ccacaggcca taatcggatg gaccggtatc
     4561 catgccccta ccctttcctc gccactttcg tgagcctcga ctctatcagc ggaccctact
     4621 ccacgatcag ctttccacgg aacattccca tctggcaggt gaagtcgtac tccccgggcc
     4681 tctcgggcat gaactcgatg ggcacgatct cgccttcggg cagtgtggcg ctcttgttga
     4741 aatctccgaa gaccaccatc tcggagcagg ccgccgactc ctccctgcgg aagctgagcc
     4801 gaactggctt gccgtgctgc accacgataa cgtcgggggt gtacccaccc ttcaccagca
     4861 ccatagcctc ctggtaaccg ctggaggtta ccgcggcgcg gaaacctccc ttccgtggtc
     4921 cccagaagaa ccacgctatg cccacgatag tcaccagtcc cccaagaatg acgattatct
     4981 tgtcggcagt cattatcttg cctccttcgc cttccagcgc ttgagccggt tggcgttgcc
     5041 gatcacagtc accgaactga agctcatcgc cagggctgcc agcagcggcg acagaagtat
     5101 tccggtgaag gggtagagca ctccgagcgc cacagggatc cccagcccgt tgtagacgaa
     5161 ggctcccaac aggttctgat acacgtttct catggtagcc ttggagatat cgacggcgga
     5221 caccactccc ctgaggcttc cgctgatcag ggtcacgtcg gctgcctcaa ttgctacgtc
     5281 ggttcctgtc ccgatggcga agcctacgtc ggattgggca agggcagggg cgtcgttgat
     5341 gccgtcgccc accatgccaa ccttctttcc ctccagttgt agcttctgga cgttgtgcgc
     5401 cttgtcctga ggcagcacct ccgccagcac ccggtcgatc ccgacctgac ggccgatggc
     5461 cttggccgtc cttgggttgt cgcccgtgag catcaccact tcgagcccca gggccttcag
     5521 ggtcgcgatg gctgccttgg agtcttcctt caccgtgtcc gccaccgcta cgatgccagc
     5581 cgcggtgccg tcgatggccg cgaacattgg ggtcttgcca tcgtccgcca gccgctgtgc
     5641 ttccagttcg agccgaccca atgccaccgc ctggccggcc atcaacttgg cgttgccgag
     5701 aagcaccttt cgcccctcca cgcgcgcctc cacgccctgg ccgggaatgg cgttgaagtc
     5761 cgagatctca ggtagcgcca acccctggac cttcgcgccg tccacgatgg cggtggccag
     5821 tgggtgctcg gagttgcgct ccaccgcagc cgagatacgc aggatgtcct cttcctggaa
     5881 gccgttgtcg gccaccacgt tggtgagctc cggcttgccc ttggtaatgg tgcccgtctt
     5941 gtccaatatc aaggtttgga gacgggatgc agtctgcagc gcctcgcccg aacggatcaa
     6001 tattccgttc tgcgccccca accccacgcc ggtggtgagg ctcatcggtg ttgccatgcc
     6061 cagagcgcag gggcaggcga tgatcaacgt ggtcaccgcc acgatcaagg cgaaggccag
     6121 gtgcggcgga ggcccgaaca cgtaccagac cacgaagcca aagaccgcca ggatcatgac
     6181 agcgggggtg aaataacccg agaccacatc cactattcgc tggatgggta ccttcgaccc
     6241 ctgagcgtcc tggaccatcc ggacgatgtt ggccaacgcc gtgtccttgc ctaccctggt
     6301 cgccctgaac ttgaagctgc ccgtggcgtt cagggtcgcc cctatcacct cgtcgtcgac
     6361 ccccttggcc accggtatcg actcgccggt gatcatgctc tcgtccacag ccgactggcc
     6421 ctccaggacc acaccgtcga ctgggatctt ctccccagga cggaccagca ccacatcccc
     6481 caccagcact tcctctacag ggatgtctac ctctcgcccg tcgcggatca cccgtgccgt
     6541 ctttgcctgc aatccgatca gcttcttgat cgcgtcggag gtgcgcccct tggccttgac
     6601 ctccattgcg agccccagca ccacaagaga ggtcaccact accgtaacgt cgtagtagac
     6661 gtcgacgaac tcggcagatg ggaagacctg gggaaacagc agcgcgatgg tggagtagag
     6721 ccaggcaacg cccgtgccga cggcgatcag cgtgtgcatg ttggccgagc ggtgcatgag
     6781 cccctgccac atgccggtga agaactgatt gccgctgtag accataactg ccaggctggc
     6841 cagcgccatc agcccccaca caatcctcag ctcgttgctc ccacggacca gcacatctcg
     6901 cagcccaggg aacaggaagg gataggacaa ggccatggtg gggatggcga ccgcggcccc
     6961 gaaccaccac ttccgcatca gttgccggta ctcctggccc tgctctgagc tctccttgtc
     7021 gagccccggc tcgctggcgg gagccgcggg agcggccttg tatgggccgg cattctccac
     7081 ggcgtgggtt agtgactcca ggttcacgga ggaggggagg taatctacct tcacctcttc
     7141 cgtggccgca ctcagggtgg catccaggac gccgggagtg gccttcaact cgtcctcgat
     7201 gactgaggcg cactcagcgc agtagagccc agacaccttc agccgtatgg atgcggtgcc
     7261 ggtgtcgtag cccgcctggc ggaccgcctc caccaattgc ccgacaccta tcagggttgg
     7321 gtcataatcg actcggacgc gcttgccttc cacatcaacc ctgacctttt ccacgccggg
     7381 cgctgtctgc agtgcctgct ccagggcggg cccgccggat ctcgtaagtc gggcaaccgg
     7441 caggtctatg ctcaccggcc cgaagaccct ctctgctaca ccagtggtgg cagacggcgc
     7501 cccattctcg gtcgaccgcc gttttgtatc catcgttacc atcttgatcc tctcgaagag
     7561 aaggggacat cgagcccctg cgtccttgta ctgcctattg tcacgcccta ccctgaggca
     7621 ttcctgaacc gatgctgagc acgaactgag tgatctatcc accttgcaga gcgcgagcca
     7681 tatggatgga ctgcttgcgt ggacgtgctc aggcgaggga agatgaaagc ccgcagcaat
     7741 gtgggattgc gtactgagga gtcggttgag gatcagacga tgggggagaa gagctaatga
     7801 gtccgcggga ccacaatgcc ggccgtaggg gcaggagcat ccactactct tgctcccgca
     7861 gcgcatatcc gacgcctctt gccgtgtgga tcattcgtgg ttcccccgcc gcttccagtt
     7921 tctgtctgag ctgcctcaca tacacttcca ggatgttggt gttaccctcg aaatcgtagc
     7981 cccagacgtg atccatcagg atctctctag tcagaactcg gcgcggctgc ctgaggaact
     8041 cgtagaggag gtcgtactcg gtcttggtaa gctcgatctc gcgatcccct cgccgcgcga
     8101 gcctggtccc ggcgtcaagg ctgaggtcct cgaaccgcaa gatctccggc cgatcgggct
     8161 cgtaccggcg caacgccgcc tgtactcggg ccagcaacac gccgaaagtg aagggcttaa
     8221 ccacgtagtc atcggctccg agttcaagcc cctggatctg gtcggcgggg gcgtctctgg
     8281 cggtcagaaa gagaactggg agccggggat ccgctgcatg cagccgtcgg agaacctcca
     8341 gcccatccac gccaggcatc atgatgtcca ggatcaccag atcgggaacg cgctccctgg
     8401 cgatctctag tccctgactt ccaccgctgg ccgtgtcgac gacgaagcct tcgtaagaca
     8461 ggccccgttt caggacactg gtcacggccg ggtcgtcatc gattaccagg atgcgcttca
     8521 tgggatggga actcctttcg tctgagtggt ccgtcgtcgc cagagcatcg gagaggccgg
     8581 gctccggcat caactgagac aaatggtacc acataggacg gttggagctt gctccaactt
     8641 ggggtggccg acgtggtcgc tgcctcacgt cggaggggaa catgcgggaa ggtggcccaa
     8701 gttggaccac cttcccgcag cggccaggct tcaccatcgc cccggagtcg cctcacttct
     8761 tgcctgcgta cttctgaggc tccttgtcga aggctgcttt gcagccgggg gagcagaagt
     8821 agtatgtctg cccctggtac tccgacttgg ccgccgcctt cttctcgtcc acgttcattc
     8881 cgcaaactgg gtctttcgcc atccccttca cctactttcc tcgacaattc ttcctgcacc
     8941 ggacaggacc ccgggtttcc agctcagtgg catcccccag aatggccacg gcttcccgag
     9001 gccacaggct ccttctcttt ctcctcgccc ttctcctcct ggcgaggccc cggcaggctt
     9061 cgctggctct ggtccggctc gcgatcctgc tgcccgtgct ggtgtcctcc gcagcagccc
     9121 atcccgccgc ccgagccatg catccgcatc atcaacacaa acagtgccgc caacacaatc
     9181 cacgatgcgt tggcctggat gaactcgatc acggtgaagt tcctcgccac atttcacttt
     9241 cgcagccgct gcagcagcgc gaagtacgca aggcccgtga cggccatggc gccgaccaca
     9301 accagggtct gcaacggcag agagagacct acgaccgggt tggccacaat gcctgccaca
     9361 ggcaccacta gtggatacag tcgcctctcc ggtggaaggc ggagggccga caggttcatg
     9421 acccagtagt agaccaggag tgccaggctg ctggcctcca gcagaggccg cagatcggcg
     9481 aaggccgcca gtagcacaag cacgctacca gcggccagca cggcgttgcg cgggatcagt
     9541 cctgggcccg acaaccggcc gaagcaggca ggaaggtcgc cgttgcggcc catcgcgaag
     9601 gccaggcgcg atagacccca gatctccgtc agcagcacgg caaaggaggc gagaagagcg
     9661 cccaccgcta acagagtcgc gccggccgga cttcccgctg ccaccatcgc cgctcgtagc
     9721 ggtgccgcct cctcccctag ccgctccggt cccagcacgc ccaaagcggc gaaggcaacc
     9781 ccgaagtaca acagcatcac gatccccacc gccgtcggca ccgctcgtgg cagcgtccag
     9841 gccgggtccc gcacctcctc agccacagtc acgggacgag cgtagccggt gtaggcgaag
     9901 aagagcaggg cgctggcctg gaggaagccc ctggcaccgc cgaggacaaa gggccgcaga
     9961 ttgtgcggag tgaaagccgg cagggccagc ccgacaaagg ccagcaggat cgcgacgttg
    10021 atgaccacga gcgccacgct cacacggcca gagatacgaa ccccaagcat attcgaaatg
    10081 accgccacgg ccacaagggc aacgccaaca gccctgagcg gaacccccgg caggagcgcc
    10141 tccaggtagg ccccgaaggt aagggcatag gtgccgcctg cggtgagggc cgcgaacagg
    10201 aagagccagc cggcgacgaa ccccacggcc ggtgtcacca ggttgcgggc gaactggtag
    10261 gcgccccccg ctcgaggatc gtaggctccc aactctgccg aactgagacc attgatggtg
    10321 gccacgacgg ctcccaacac gaccgctacc agcagcgacc cgccggttgt ccccgcagcc
    10381 tgccccatcg acacatagac gcccgctcct agcatggcgc ccagcgcgac cgccatcgtc
    10441 tggccgactc ccaactgtgg agtcaggcga ggctcctggt tattgtcggc caagttgcgg
    10501 ttatccactg cgactcaaca ctatcgatgg gagagactac gctgccgtac tcggcaggtc
    10561 cttcagccca agcgggcagc aagtcacccg cagaccggaa ggtggcgggt atctgagacg
    10621 cacggctgct ggaagctagg gttttctcca gtcgatggcg tagatgtagc ctccggccag
    10681 gtagtgaagc tgtgggtttg gatcagggat caggtgcatg ggggggcgaa gcctcgggat
    10741 cagacgaagg attgcccccg acaccagtcc gccgaaacct gtacccggag gcgctgtctc
    10801 gtcagatacc acgatcttcg cacccggttt ggccacacgg gacatctcct ccaaggctcg
    10861 gcgggggtcc gtaaagaagt tgatgccgcc caggtggagc accgcgtcga aggagttgtc
    10921 gggatacggc aggtcctctg cctgcccttg ttggaggtcc accgggcacg gcatggtcgc
    10981 gaccttctta cgggcttgtg ccagcatccc ctcgctcaga tcctgagcgt agatgtgacc
    11041 cgaggagccc acctttgcag ctatgaaggg gaagttggcc cccgtgcccg cacccacctc
    11101 caatacctgg tcgccgggct ccaactccat catctccgcc aattgccggc gcaacggagt
    11161 gagggcgctc tgctcccccg cccacagatc gtagattggg gcgacgaggt tgtacattgc
    11221 tcggaagaga cggttggggt cccgtcgcca cgtgggcccc ttggcgagta cgatcaccag
    11281 caggccggtc gccaagacgc cgaccaacga cggcctgtag agtccagact tgcggtgcgc
    11341 tgcctattcc cgctatggac ccgcggtggc ccttgcagcc acggagaact tcgaggcggt
    11401 gacccggctc ggtgtgatcc tatcgcccac caacaaggat gccacggtcg tccccgagcc
    11461 tgtagatcgg ccggccg
//
LOCUS       DATALS010000551         4101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_134283, whole genome shotgun sequence.
ACCESSION   DATALS010000551 DATALS010000000
VERSION     DATALS010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134283"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..991)
                     /locus_tag="VHS06_12255"
     CDS             complement(113..991)
                     /locus_tag="VHS06_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2988924.1"
                     /translation="MTSLALALVLSSAVLHALWNLLYKRARDKPSFAFLFGVSATVIY
                     LPVFLLMVGGKQIVWQGLAVAVLSAFVHTLYFTFLGRGYSVGDLSLVYPLARGTGPLL
                     VPIWATIFLGERITAIGLVGILAVVGGVYIIHLRELSLSGLLAPLRSLSTRPTQIAIF
                     SGLLISIYTVVDRVGVGYMDPVIYMYVWTALFTAMYGPYVYLTSGPVKIRTEWRDNRI
                     AIVAVGILMVLTYTMVLFAMTMSNVSYVAAAREIAVVFGAAMGTVMLRESYGRSKMGG
                     SLLIALGVALIGMAKG"
     gene            complement(1080..1217)
                     /locus_tag="VHS06_12260"
     CDS             complement(1080..1217)
                     /locus_tag="VHS06_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988925.1"
                     /translation="MDNQTPATEGITPHRRGRLAKLSGGIHVGFYSSKTLIEDGKKQQ
                     K"
     gene            complement(1471..3555)
                     /gene="ligA"
                     /locus_tag="VHS06_12265"
     CDS             complement(1471..3555)
                     /gene="ligA"
                     /locus_tag="VHS06_12265"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX2988926.1"
                     /translation="MEGSDLGAAQERVAELRKQINYHNYRYYALDAPEISDGEYDRLM
                     EELKELEAEHPELSSPDSPTQRVGAGPLESFNVVHHEIPMLSLANVFSQEALLKWYQR
                     ISGLVSREPRDLVVEPKIDGLAISLTYVDGHFTVGATRGDGIQGEDITQNLRTVRSVP
                     LVLNDHAPARIEVRGEVFLPKKGFQRVNEERMAQGQSLFANPRNAAAGSVRQLDPRVT
                     AGRPLDVFVYTLGQLVNGEQPATHWGALELMKSLGFKVNPLAKLFHSIEEVMEFCAGW
                     EEKKETLDYEIDGVVVKVNDLELQREMGEVAREPRWAVAYKFPPTQATTRLKEIHINV
                     GRTGSLNPWAELEPVRLGGVTIQKATLHNEEDIHRKDIREGDWVLIQRAGEVIPQVVG
                     PILSKRTGEEHEFNMPEKCPECGGPVVKPEGEAMHRCTNTLSCPAQQFELLKHFVSRP
                     AMDIEGIGESLCASLIAAGLVHDVADLYYLEMDQLIGIERMAKKSAQNLLDALKESKE
                     RPIAKLIFALGIRYVGEETARLLANAFGSLDALMKADYDRVVAVEGVGPKIARSVVDY
                     FANERNLAVIEKLRAAGLRFQDEPEEPKGNLPLAGKQFVITGTLQGLSRLQAEEMIRQ
                     LGGEAGSSVTRKTDYLVVGVDPGSKLQKAQSLGITILTDRQFQEILQQHTTPIESKQE
                     GVDHPVQPGLGI"
     gene            complement(3646..>4101)
                     /locus_tag="VHS06_12270"
     CDS             complement(3646..>4101)
                     /locus_tag="VHS06_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988927.1"
                     /translation="LVKVAVVGVVMISAGLTAYDMVDLHPYQYIYFNRLVGGGLETAS
                     TKYETDYYGVSYREGVRWVIDNYHPANGGKVRVANPSLNFLTAYYLEASPELRDKFQP
                     VQPWDNPDVFIAITRWNFHNDKEGTVLHVISRKGVPLLYVIKPQPQPAG"
BASE COUNT          730 a         1312 c         1235 g          824 t
ORIGIN      
        1 tcagcactca gcactcagca ctcagcactc agcactcagc actcagcact cagcactcag
       61 cactcagcac tcagcactca gcactcagca ctcagcactc agcactcagc actcacccct
      121 tcgccatccc gatcagcgca accccaagag cgatgagcag cgagccgccc atcttggacc
      181 gaccgtagct ttctctcagc atgactgtcc ccatggccgc tccgaatacg accgcgatct
      241 cgcgtgcggc cgcaacgtag ctcacgttgc tcatggtcat agcgaacagc accatggtgt
      301 atgtgagcac catcaggatg cctacggcga cgatggcgat cctgttgtcc ctccactcgg
      361 tccttatctt gacgggtccg ctggtcaggt acacgtatgg gccgtacatg gccgtgaaca
      421 gcgcagtcca gacatacatg taaatgaccg ggtccatgta gcccacccca accctgtcca
      481 ccacggtgta gatggagatc agcaggccgg agaagatggc gatctgggtg gggcgggtgc
      541 tcagggagcg caagggggcc agcaggccgg acagcgagag ttcccgcagg tggatgatgt
      601 acaccccgcc caccactgcc agtatcccga ccagccctat ggctgtgatc cgctccccga
      661 ggaagatggt ggcccagatg ggtaccagca ggggtccggt acccctggcc agcgggtaga
      721 ccagggagag gtccccgacg ctgtagccgc ggccgaggaa ggtgaagtag agggtgtgga
      781 cgaaggcgga gagcacggcc acggccagcc cctgccagac gatctgcttc ccccctacca
      841 tcaacaggaa gaccggcagg tagatgacgg tggcagatac cccgaagagg aaggcgaagg
      901 agggcttatc cctggcgcgt ttgtagagca ggttccacag ggcgtggagg acggcggagg
      961 agagtaccag ggcgagggct aagctggtca cggtatgccc ccagaacatt ggtaggggcg
     1021 acgcatgcgt cgcccctatc gctagcctat cgctgtatcg gactgcctgt caatcaggac
     1081 tacttttgct gcttcttgcc gtcttcgatc agcgtcttgg aggagtagaa accgacgtgg
     1141 atcccgcccg agagctttgc taaacgcccg cgacggtgag gtgtaatacc ctcggtcgcg
     1201 ggcgtctgat tatccatgca gtcctaaccc gaaaccgtcg atcctacatc ggcgaggaag
     1261 ataggccaac cgctcccagg gtggggctcg gcgagtctat cctccctcgt tgacgtggaa
     1321 ttgaccttcc caggatgcgg catcgcagtt ccctcacccc ttgccgtcga gtgcttcgcg
     1381 tcatcgggat agggcatggc gaacctcccg ttcacgcatg cgagtcgatt gcatacccat
     1441 ataacggatt cctcgatgta ctcccgggga ctaaatcccc agtccgggtt ggacagggtg
     1501 gtcaactcct tcctgcttgg actcgatggg cgtagtgtgc tgttggagaa tctcctggaa
     1561 ctgccggtcg gtcaggatgg tgatccccag cgattgggcc ttctgcagct tggagccggg
     1621 gtctacgccc accaccaggt agtcggtctt gcgggtgacg gagcttcccg cctcgcctcc
     1681 cagttgccgg atcatctcct ccgcctgcag tcgcgagagt ccctgcagcg ttcccgtgat
     1741 cacgaactgc tttcccgcca gggggaggtt tcccttcggc tcctccggct cgtcctggaa
     1801 gcggagcccc gccgccctca gcttctcgat cactgccagg ttgcgttcgt tggcgaagta
     1861 gtccaccacg gagcgggcga tcttcggccc gaccccctcc acggccacga ctcggtcgta
     1921 gtcggccttc atcagcgcgt ccagactccc gaaggcgttg gccagcagcc tcgccgtctc
     1981 ctctcccacg tagcggatcc ccagggcgaa gatcagcttt gcgatcggcc gctccttgct
     2041 ctctttcagg gcgtcgagca ggttctgggc actcttcttc gccatccgct ctatgccgat
     2101 cagttgatcc atctcgagat agtagagatc ggccacgtcg tggacgaggc ccgccgcgat
     2161 cagggaggcg cacagcgact cgccgatccc ctcgatgtcc atcgccggcc tggagacgaa
     2221 atgctttagc agctcgaact gctgtgccgg acaggacagt gtgttggtgc agcggtgcat
     2281 tgcctcgccc tccggtttca ccactggacc gccacattcg gggcacttct cgggcatgtt
     2341 gaactcatgc tcctccccgg tgcgcttgct gaggatgggg ccgaccacct gggggatcac
     2401 ctcccccgcc cgctggatca gcacccagtc gccctcgcgg atgtccttcc ggtggatgtc
     2461 ctcctcgttg tggagtgtgg ccttctggat ggttacccca cccagccgga ccggctccag
     2521 ctctgcccag gggttcaggc tccctgtgcg ccccacattg atgtgtatct ccttcagcct
     2581 ggtggtcgcc tgggtcgggg gaaacttgta ggccacagcc cagcgcggtt cccgggccac
     2641 ctcgcccatt tcccgctgca gctccaggtc gttcaccttc acaacgacgc cgtcgatctc
     2701 gtaatccagc gtctccttct tctcctccca gccggcacag aactccatca cctcttcgat
     2761 ggagtggaac agcttggcca aggggttcac cttgaagccc agggatttca tcagctccag
     2821 ggcgccccag tgggtcgccg gctgctcccc gttgaccagt tgtccgagcg tgtagacgaa
     2881 gacatccaac ggccggccgg cggtcacccg tgggtcgagc tggcggacgg agcctgcggc
     2941 ggcgttgcgg gggttggcga agagggactg gccctgggcc atccgctctt cgttcactcg
     3001 ctggaacccc ttcttgggca ggaagacctc gccccgaacc tcgatccggg cgggggcgtg
     3061 gtcgttcagc accagcggaa cgctgcgcac ggtgcgcagg ttctgggtga tgtcctcacc
     3121 ctggatgccg tcgccgcggg ttgcgcccac ggtgaagtgg ccgtccacgt aggtgaggga
     3181 gatggccaga ccgtcgatct tcggctccac taccaggtcg cgcggctcgc ggctcaccag
     3241 cccggagatg cgctgatacc acttgagcag cgcctcctgg gagaagacgt ttgcgaggct
     3301 gagcatcggt atctcgtggt gcacgacgtt gaaggattcc agtgggcctg cgccgacccg
     3361 ttgggtgggg gaatctggcg aggagagctc aggatgctcc gcctccagct ccttcagttc
     3421 ctccatcagc cgatcgtact cgccgtcgga gatttccgga gcgtccaggg cgtagtatcg
     3481 atagttgtgg tagttgatct gcttgcgaag ctccgcgacc cgctcctggg cggctccgag
     3541 atccgatcct tccatctgca cagcccctca gcctattact aagggaagga taccacacca
     3601 actcgggagc ttgatctacc tgcagatcaa cgatacaggc gtggattagc cggcgggctg
     3661 tggttgtggt ttgatgacat acaggagggg aacaccttta cggctgatga cgtgcagcac
     3721 tgttccctcc ttgtcgttgt ggaagttcca tcgggtgatc gcgatgaaga cgtcgggatt
     3781 gtcccatggc tggaccggtt ggaacttgtc tcgtagctcc gggctcgcct ccaggtagta
     3841 ggcggtcagg aagttcagag aggggttcgc cactcgcacc ttccctccgt ttgccgggtg
     3901 gtagttgtcg attacccacc gcacgccctc tctgtaagag accccatagt agtctgtctc
     3961 gtacttggtc gatgccgttt ctaggccacc cccgaccaag cgattgaagt agatgtactg
     4021 gtaggggtgc agatcgacca tgtcataagc cgttagcccg gcagagatca tcacgacgcc
     4081 aaccacggct acctttacta g
//
LOCUS       DATALS010000552         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970
            k141_134997, whole genome shotgun sequence.
ACCESSION   DATALS010000552 DATALS010000000
VERSION     DATALS010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134997"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /gene="arsB"
                     /locus_tag="VHS06_12275"
     CDS             complement(<1..1072)
                     /gene="arsB"
                     /locus_tag="VHS06_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX2988928.1"
                     /translation="MSETTDSRAVPAEKNVVGRLSFLDRFLPVWIFAAMGFGLWLGYS
                     SPGVIKALDQARIDTVSFPIAIGLLWMMYPVLARVKYEELGKIKSAGRMFTLSLIENW
                     VIGPLLMFTLAWLLLPDMPEYRTGLILIGLARCIAMVLIWNMLAGGDNEYCAVLVALN
                     SIFQVLAYSALAYFYITVLPGWLGLGVGESIHIEFWDIAKSVLIFLGIPLVAGMITRY
                     VGLHTRGKEWYDRVAMPKLAPTVLLALLFTIVVMFSMKGDVILRLPMDVVRIAIPLLV
                     YFGLMFFVSFYLSWKLGFKYPESATLSFTVASNNFELAIAVAVGIFGFASGQALAAVV
                     GPLIEVPVLIGLVYVALALRERL"
     gene            complement(1146..1484)
                     /locus_tag="VHS06_12280"
     CDS             complement(1146..1484)
                     /locus_tag="VHS06_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016952146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2988929.1"
                     /translation="MSGHSQTEVAPDLLPALQALADPSRLKIVHMLHEREQCVCHLTE
                     ALDLKQSTISHHLSVLKKAGLVVDRRDKKDARWSYYSLSPKAVVLGQRIAALLDAGSV
                     DPTPADCSGR"
     gene            1744..2493
                     /locus_tag="VHS06_12285"
     CDS             1744..2493
                     /locus_tag="VHS06_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2988930.1"
                     /translation="MVAACALLAAVSLLVQLRLLANWDKSALEWIVAHRVGEASDVMN
                     WVFRLGFAQLDAAIAVIWAAWALIGSESRSRSQRRTVPATQGNPWISLRTRMIPLAPL
                     MVFVVIGVQAGLRLVINQPAPGAAYELHREYSSEPVGLTLDRTDAAAREAFATATAIQ
                     ATPISARGSYPSGHASRVLFLSLLATRTVRGRGKVSRATRTAVVVLPTALVGYSALYF
                     GYHWPSDLLGGALLALAIYAAVEHLAARIRR"
BASE COUNT          537 a          796 c          738 g          452 t
ORIGIN      
        1 acaacctctc cctgagagcc aacgccacgt acaccaagcc gatcagcacc ggcacctcga
       61 tcaacgggcc caccacggcg gccagcgcct gccctgacgc aaagccgaag atccccaccg
      121 caaccgcaat cgccagctcg aaattgttgc tcgctactgt gaacgacagg gtggcagact
      181 cggggtactt gaaacccagc ttccaggaaa ggtagaagga gacgaagaac atcaggccga
      241 agtagaccag cagcggtatg gcgatcctga ccacgtccat ggggagtcgc aggatcacgt
      301 cacccttcat ggagaacatc accacgatgg tgaacagcag cgccagcagc acggtcgggg
      361 ccaacttggg catagccacc ctgtcgtacc actccttgcc acgcgtgtgg aggccgacgt
      421 agcgggtgat catccccgcc accagcggga tgcccaggaa gatcagcacg ctcttggcga
      481 tgtcccagaa ctcgatgtgg atcgactctc cgacccccag accgagccag cccggcagga
      541 cggtaatgta gaagtaggcc agggcggagt aggccaacac ctggaagatc gagttgaggg
      601 cgaccaggac ggcacaatac tcgttgtcgc ctcctgccag catattccag atcagcacca
      661 tggcgatgca ccgggccagg ccgatgagga tcagtcccgt ccggtactcc ggcatgtcgg
      721 gcagcagcag ccaggcgagg gtgaacatca gcaagggacc gatcacccag ttctcgatca
      781 gggatagtgt aaacatccgc ccggcgctct tgatcttgcc caactcctcg tacttcaccc
      841 tcgcaaggac ggggtacatc atccagagca ggccgatggc gatgggaaag gaaacggtgt
      901 cgatcctcgc ctggtccaac gccttgatca cgccggggga ggagtagccg agccaaagcc
      961 cgaagcccat ggccgcgaag atccacaccg gaagaaaccg atcaaggaac gagagccggc
     1021 caaccacgtt cttctcagcc ggcaccgccc ggctgtcagt cgtctcagac acgctagtca
     1081 cctcctcgtg gttttcgatg ctgtaagaga gttctcctat gctcagctac catttcctct
     1141 ttcagctatc ggcctgaaca atcggctggg gttggatcca cacttcctgc gtccagcagg
     1201 gcggcaatcc tctgcccgag cacgactgcc ttgggagaca gggagtagta gctccagcgg
     1261 gcatccttct tgtcccgccg gtccacgacg agcccggcct tcttcaagac ggacagatga
     1321 tgggaaatgg tgctctgctt caggtccaaa gcctcggtga ggtggcagac acactgctca
     1381 cgctcatgga gcatgtggac aatcttgagc cggctgggat cggccagcgc ctggagagcc
     1441 ggcaaaaggt ccggggcgac ctcagtctgt gaatgccctg acacacacct ggacacagaa
     1501 cagcccatag gccaatcacc ccttctgaag atagatcgat gagtgtctat atgttatgtt
     1561 gaaagaagac ccccgtcaag gctcggcgca gatcggagtg tataatgacg ccatgatcat
     1621 gagtatagtt gtccccgtct cgtgctgggt gtaggtcgaa gaatgcgcca aaccgccgag
     1681 ttagctcgta caggacccgg atacgcagac gcgccggtcg agcatcgacg ttgggccacc
     1741 cgcgtggtcg cagcatgcgc actgctcgcc gccgtgtcgc tcctcgtcca acttaggcta
     1801 ctggccaact gggataagtc ggccctcgag tggattgtcg cccacagggt gggagaagct
     1861 tccgatgtaa tgaactgggt ctttcggctg ggattcgcgc aactggacgc ggccatcgct
     1921 gtgatttggg ccgcgtgggc gttgatcgga agcgagtcac gatccagaag tcagagaagg
     1981 acggtgccgg caacccaggg gaatccctgg atttccctta ggacccgtat gatccccctc
     2041 gcccctctga tggtctttgt cgtgatagga gtgcaggccg ggctgcgcct ggtgatcaac
     2101 cagcctgccc caggcgccgc ctacgagttg cacagagagt actcttcgga acccgttgga
     2161 ctcaccctgg accgcaccga tgcggccgcc cgcgaggctt ttgccacagc cacggcgatc
     2221 caggccacac caatctcggc ccgaggcagt taccccagcg gccatgcctc cagggtgctc
     2281 ttcctttccc ttctcgccac ccgaacagtt cgtggtcgag ggaaggtctc acgagccacg
     2341 cggacagccg tcgtggtatt gcccacggcc ctggtcgggt acagcgccct ctacttcggc
     2401 taccactggc cgagcgacct gctgggaggg gcacttctag cgctcgcgat ttacgcagca
     2461 gtggagcatc ttgcagcgag aattcgccgc tgataccttc caagtaggtc cctttctact
     2521 gcc
//
LOCUS       DATALS010000553         6402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_82463,
            whole genome shotgun sequence.
ACCESSION   DATALS010000553 DATALS010000000
VERSION     DATALS010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82463"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VHS06_12290"
     CDS             <1..467
                     /locus_tag="VHS06_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX2988931.1"
                     /translation="LTEEEASDRALVFDIGLLTRRVLDATVWLRKEADTRDLSIGYFG
                     ASTGAAAALSAAAQEGTRVAAVVSRGGRPDLAADALRRVQSPTLLIVGGNDEPVVELN
                     RQAYSALRAEKELVIVPGATHLFEEPGALEEVASLARDWFLRHTAARLKRAA"
     gene            625..840
                     /locus_tag="VHS06_12295"
     CDS             625..840
                     /locus_tag="VHS06_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2988932.1"
                     /translation="MAKIYFNPDYCKGCFFCIQFCPRGVFAPSGRMNRKGYVVPEVVN
                     EEACNGCGMCDRACPDFAIVIEKEAAV"
     gene            837..1997
                     /locus_tag="VHS06_12300"
     CDS             837..1997
                     /locus_tag="VHS06_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX2988933.1"
                     /translation="MTGAVKTGSYFVQGDVAIAEGAIAAGCRYFAGYPITPVSELSET
                     MAERMPEVGGICMQMEDELASIHSAAAASVGGLKSMTASSGPGISLKLEGLGWAIKNE
                     IPVVIVDVQRAGPSNGVATNVGQADFYTVRYGAAGGNAEIIALSPKSVQEAFDLTIEC
                     FNLAEVFRCPVFLLTDETVGHMRERLVIPPESEVDRQERIKPKVAPEQFLMYPLEDPE
                     AISYPFPNIGEGFGICVSPYTHTSKGYDTTTVEGQYKMARRLVAKVRAHLDMLVRVER
                     VSLEDAEIAVVSYGGVARPALSAVQQARADGIRAGMLRPIIVWPFPDGEVEAVAERVH
                     TILVPEINLDGQMVREVERAAHGKARVVPIPHAGVEPHRPVEILEQIRRFGR"
     gene            1994..2854
                     /locus_tag="VHS06_12305"
     CDS             1994..2854
                     /locus_tag="VHS06_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2988934.1"
                     /translation="MTAVSERPAKIHPLVERYLRLDRMPHMACPGCGIGNVIHCVLQV
                     MDDLQLNPQNTVWVQGVGCSSRITYSRYKGDNIDAHHGRIYGVATGLKMARPDMNFIL
                     FTGDGDSGAIGGNHFIHACRRNLDVTALMFNNGIYGMTGGQVAPTTPRGGTTTTTPYG
                     SLEDPFDMCKLAEAAGATYVARWTSLPKHYGQVVKSIKVGIENKGFSYIEVVVPCTTQ
                     FGRYVLKTEEMPKNIQWIEDHTVTLSQAEKLSIEEREGKMLLGEFVRRERTGVVQRYM
                     EMIERMGGRQ"
     gene            2851..3384
                     /locus_tag="VHS06_12310"
     CDS             2851..3384
                     /locus_tag="VHS06_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013099506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX2988935.1"
                     /translation="MSRIEARFAGFGGQGILFAGITLGRAACLYAGKHATQTQSYGTE
                     ARGGASRCDVVIDDVEVDYPGVTSPDIFVAFSQEAYDKLSGGLKPGGKLFYDPELVNP
                     DKREGVELHSVPATRSAMDELGDKLVANVVMLGAVITASGVIDMEILKRCLRETTPAR
                     RLETNLKALDLGAALVA"
     gene            3381..4622
                     /locus_tag="VHS06_12315"
     CDS             3381..4622
                     /locus_tag="VHS06_12315"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2988936.1"
                     /translation="MMDRDSCGANEKDEEVSAAMSLTGVRVLDFSRVLAGPYCTMMLA
                     ELGADVIKVEDPRGGDEAREWPPFVEGQSGYFFSVNRSKRSIALNLKAGESRRIVRDL
                     AERSDVVVENFAPGVTSRLGIDYGTLSAANPHLVYCSISGFGQTGPYRDKRAYDPILQ
                     AMSGIMSVTGSRGGEPVKCGVAVTDVASAVFAAFAIAASLYRREKTGRGQYIDLSMLD
                     TGLSMMTFMGAMYLCAGEVPTTYGSENPTRVPSANYRTSDGRWVHVVINDRQWPRFCE
                     LLGVPDLSTDPDFANNRVRVANRDRVNAKIEERMRTRTAEEWIELFDREGLPIGPVNS
                     LDRILNDPHVLAREMVKSFDHPLAGKVRAIDMPYRFSETPSMIRNAPPLLGESTDSVL
                     IELLGYSPEEVTRLRESGVIG"
     gene            complement(4688..4849)
                     /locus_tag="VHS06_12320"
     CDS             complement(4688..4849)
                     /locus_tag="VHS06_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007472125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX2988937.1"
                     /translation="MLGSILWAIIVALLIFWIIGAFFANLGNIVWIALVVAVILIVYN
                     LLTSGRAEV"
     gene            5262..6185
                     /gene="argF"
                     /locus_tag="VHS06_12325"
     CDS             5262..6185
                     /gene="argF"
                     /locus_tag="VHS06_12325"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX2988938.1"
                     /translation="MRSILSVADLTRKEFTDVLERASVLKEETKRGQWRSELEKKTVA
                     LLFQKPSLRTRVSFEQCIRQLGGNSLYLSPAEVGLGEREAVQDVARVLCRYVDCVVAR
                     VKRHTDLQKLAQYSSVPVINALSDLSHPCQAMADLLTIREKKDRFDGLVLSFIGDGNN
                     VAHSLILASAKVGMHIRIACPVGFEPNPDIIERSNADRAATGATFTIVHSPQEAAAGA
                     DVLYTDVWASMGQEAEIDQRRRKFAGYKIDADLLRLAKPDAVVMHDLPAHRGDEITDD
                     VIDGPQSVVFDQAENRLHAQKGLLSWLFETK"
BASE COUNT         1257 a         1830 c         2110 g         1205 t
ORIGIN      
        1 tgttgaccga ggaggaggct tcggatcgcg ccctcgtgtt cgacatcgga ctgcttactc
       61 gccgcgtgtt ggatgcgacg gtctggttgc gcaaagaggc ggatacaagg gacctctcca
      121 tcggctactt cggggcgagt acgggagcgg ccgcggcact cagtgccgcc gcacaggagg
      181 gaacgagggt ggcggctgtg gtgtcgcgcg gaggccggcc ggacctggca gccgacgcgt
      241 tgcggcgagt tcagtctccg accttgctga tcgttggggg gaatgacgag cccgtggtgg
      301 agttgaaccg gcaggcgtac tctgcactga gagcggagaa ggaattggtg attgtccccg
      361 gtgccaccca tctcttcgag gagcccggag cgttggagga ggtggcttcg ctggcgagag
      421 actggtttct gcgtcataca gccgccaggc tgaagcgggc agcataggga ggcggagaaa
      481 caacatgttg acatgacaaa tactcccacc tagaatcgtt ccattaccac tatgtggttc
      541 ctccgtcggc tgcgcggagc gaccctggct ctgcgctccc ctcctttcgc agggcccttc
      601 tccgcatggg cgagggaggt cttcgtggcc aagatctact tcaacccgga ttactgcaag
      661 ggctgcttct tctgcattca gttctgtcca agaggcgtct tcgccccttc gggtcggatg
      721 aaccggaagg ggtacgtggt tccggaagtg gtcaacgagg aggcatgcaa cggctgcgga
      781 atgtgcgacc gggcctgccc ggacttcgcc attgtgatcg agaaggaggc ggcggtatga
      841 cgggcgccgt caagaccggt agctacttcg ttcagggtga cgtggccatc gcggaggggg
      901 ccatcgcggc cgggtgccgc tacttcgccg ggtatcccat cacgccggtc tccgaactct
      961 ctgaaaccat ggcggagcgg atgcctgagg tgggcgggat ctgcatgcag atggaggacg
     1021 aactggcctc catccactct gccgccgcgg cttcagtcgg cgggctgaag tccatgaccg
     1081 cttcctctgg gcccggcata agcctgaagc tcgaggggtt ggggtgggcc atcaagaacg
     1141 agatcccagt ggtcatcgtg gacgtgcagc gcgccggacc gagcaacggc gtagccacca
     1201 acgtcggcca ggccgacttc tacacggtgc gctacggggc cgccggcggg aacgccgaga
     1261 tcattgccct ttcccccaag tctgttcagg aggcgttcga tctcaccatc gagtgcttca
     1321 atctggcgga ggtgttccgc tgccccgtgt tcctgctcac cgacgagacg gtgggccaca
     1381 tgcgtgagcg attggtgatc ccacccgaga gcgaggtgga ccgccaggag aggatcaagc
     1441 cgaaggtcgc tcctgaacag tttctgatgt atcccttgga ggatccggaa gccatctctt
     1501 accctttccc caacatcggc gagggtttcg ggatctgcgt ttccccctac acccatacct
     1561 ccaagggcta cgacaccact accgtcgagg gccagtataa gatggcccgg cggctggttg
     1621 ccaaggtgcg tgcccacctg gacatgttgg tgcgagtcga gagggtatcc ttggaggacg
     1681 ctgagatcgc cgtggtttct tacggcgggg tggcgaggcc cgcgctctct gccgtacagc
     1741 aggcaagggc tgacggcatc agggccggca tgctgcgccc catcatcgtc tggccgttcc
     1801 cagatgggga ggtcgaggcg gttgccgagc gggttcacac gatcctggtg ccggagatca
     1861 acctggatgg gcagatggtg agggaggtgg agcgggcggc tcacggcaaa gctcgcgtgg
     1921 ttcccatccc ccatgccggc gtcgagcccc accgaccggt ggagatcctg gagcagattc
     1981 ggaggttcgg gcgatgactg ctgtgtccga gagacccgcc aagatccacc ctctggtgga
     2041 gaggtatctg cgactggacc gaatgcccca tatggcctgc ccgggctgcg gcatcggcaa
     2101 cgtgatccac tgcgtgcttc aagtcatgga cgaccttcag ttgaaccctc agaacacggt
     2161 atgggtccag ggtgtgggct gcagctctcg catcacctat tcgcgataca agggcgacaa
     2221 catcgatgct catcacggca ggatatatgg ggtggccacg gggctgaaga tggcccgtcc
     2281 cgacatgaac ttcatactgt tcacgggtga cggcgactct ggggccatcg ggggcaatca
     2341 cttcattcat gcctgtcgtc gcaacctgga cgtgactgcg ctcatgttca acaacgggat
     2401 ctatggaatg accggcgggc aggttgcccc gaccacgccg cgaggcggaa ccacgacaac
     2461 gactccgtac ggcagcctgg aggatccctt tgacatgtgc aagctggccg aggcggccgg
     2521 tgcgacctac gtggcccgct ggacatccct gccgaagcac tacggccagg tagtgaaatc
     2581 gatcaaggtg ggcatcgaga ataaggggtt ttcctacatt gaggttgtcg tgccctgcac
     2641 gacccagttc ggacgctatg tgttgaagac cgaggagatg ccgaagaaca tccagtggat
     2701 cgaggaccac accgtcaccc tttcccaggc ggagaagctc tcgatcgagg aacgtgaggg
     2761 caagatgctg ctgggtgagt tcgtcagacg ggagcgaacc ggggtcgtgc agcgctacat
     2821 ggagatgatc gagcgtatgg gaggccggca atgagcagga tagaggcacg ttttgccggt
     2881 ttcggcgggc agggcatcct gttcgcgggt atcaccctgg gcagggcggc ctgtctgtac
     2941 gcgggcaagc atgccacgca gacacagtcc tacggcaccg aagcgcgcgg cggcgccagc
     3001 cgctgcgatg tggtgatcga cgacgtcgag gtggactacc ccggcgtcac cagcccggat
     3061 atcttcgtgg ccttctccca ggaggcgtac gacaagctgt ccggcggtct gaagccgggt
     3121 ggcaagctgt tctacgaccc cgagcttgtc aatccggaca agcgggaagg ggtggagctt
     3181 cactccgtgc cggcaactcg ctctgcgatg gacgaactgg gcgacaagtt ggtggcgaat
     3241 gtggtcatgc tgggcgcagt gatcaccgcc agcggtgtga tcgacatgga gatcctgaag
     3301 cggtgcctga gggaaacgac cccagccaga cggctggaga ccaacctgaa ggcgctcgat
     3361 ctgggggccg ccctggtagc atgatggacc gcgatagttg cggggccaac gaaaaggatg
     3421 aggaggtttc ggcggcgatg tcgctgactg gagtgcgagt gcttgatttc tcccgggtcc
     3481 tggcagggcc ctactgcacg atgatgttgg cggagttggg ggcggacgtc atcaaggtgg
     3541 aggatcccag gggaggggat gaggcgaggg agtggccacc cttcgtggaa ggacagagcg
     3601 gctacttctt ctcggtcaat cggagcaaac ggagcatcgc actcaacctc aaggcggggg
     3661 agtcgaggcg gatcgttcgg gatctggcgg agcggtcgga cgtggttgtg gagaattttg
     3721 cgcccggggt gacctctcgc ctggggatcg actacggcac cctctccgct gccaatcctc
     3781 atcttgtgta ttgctctatc tctggtttcg gccagaccgg gccctatcga gacaagaggg
     3841 cctacgatcc gatccttcaa gccatgagtg gcatcatgag tgtcaccggc tctcgcggcg
     3901 gggagcccgt gaagtgcgga gtggcggtga ccgacgttgc cagcgccgtg tttgccgcct
     3961 tcgccatcgc agccagcctc taccggaggg agaagacggg tcgaggtcag tacatcgacc
     4021 tcagcatgct ggatacgggc ctatccatga tgaccttcat gggagccatg tacttgtgtg
     4081 cgggggaggt cccgactacc tatggatcgg agaaccccac cagggtgccc agcgccaact
     4141 atcgaacctc ggatgggcga tgggtgcacg tggtgattaa cgatcggcag tggccgcgct
     4201 tctgtgagct attgggcgtt ccagacctgt ccacggatcc cgacttcgcc aacaatcgcg
     4261 ttcgggttgc caacagggat cgggtcaacg cgaagatcga agagcggatg cggacccgaa
     4321 cggcggagga gtggatcgaa ctgttcgaca gggaagggct gcccataggg ccggtgaaca
     4381 gcctggatcg gatactgaac gaccctcacg tattggcgag agagatggta aagagcttcg
     4441 accacccctt ggccgggaag gttcgagcga tagacatgcc ctaccgcttc agtgagacac
     4501 ccagcatgat ccgcaatgcg cctcccctgt tgggagagag caccgactcg gtgctgatag
     4561 aactgttggg ctacagccca gaagaggtca ccaggttaag ggagtcggga gtgatcggtt
     4621 aggcgttggg gatctaccgg agtggtagag cgacggcccg ggtgttgata cccgggccgt
     4681 cctcgtgtca gacctctgct cgtccgctag tgagcaggtt gtacacgatg aggatcaccg
     4741 ccaccaccag cgcaatccag actatattgc ccagattggc gaagaacgcg ccgatgatcc
     4801 agaagatcag caacgcaacg atgattgccc ataggatcga gcctagcatt gtgcaagcct
     4861 ccctaaaggt tgccaatccc gccaccacgg gaaagctgct tttggcagga gccgtgccat
     4921 ctgctggccc ctcagatcgt gatgtgcgat agaagcgggc ggtgcgcgcg ccacctgggc
     4981 ccaagcgagc cccaaagcta caattcccgc cactcatatg atgttggagc attccaatgt
     5041 cacatgggac cggccgcaac agggtagagt tatggtatac tggcggctgg ccagccacgc
     5101 tcggctccat ctagtgacgg ctggcatacc cggcatgggc gcgacaccac aagccattgg
     5161 gctttgtcgc ttccccgggt cgcgttgtcg agaaatcccc gtcgcgaccc agcccgcgcc
     5221 cttcgacgga gtacatctcc cgtattcagc agggggacca catgcgcagc atcctcagtg
     5281 tagccgatct aacgcgaaaa gaattcaccg atgttctgga gcgagcctcc gttctcaaag
     5341 aagagacgaa gagaggacag tggcgtagcg agctcgaaaa gaagacggta gcgctcctct
     5401 tccagaagcc ttctctgcga accagggttt ccttcgagca gtgtatccga cagttgggcg
     5461 gaaattccct ctacctgtcg cctgccgagg tgggacttgg tgagcgggag gcagtccagg
     5521 acgtagcgag agttctctgc cgatacgtcg attgcgtggt cgcgcgggtc aaacgccaca
     5581 ccgatctgca gaagcttgcc caatactcat cggttccggt gatcaatgcc ctgtcggatc
     5641 ttagccatcc gtgccaggcg atggcggacc tcctgacgat cagagaaaag aaggatcggt
     5701 ttgatgggct ggtgctctcc ttcataggtg acggcaacaa cgtagcccat tccctgatcc
     5761 ttgcttcagc caaagttgga atgcacattc gcattgcctg cccggtgggt ttcgagccca
     5821 accctgatat catcgagcga tccaacgcgg accgagccgc tacaggcgcg accttcacca
     5881 tagtccactc gccgcaagag gctgccgcag gggcggacgt gctttacacc gacgtctggg
     5941 ctagcatggg gcaggaggcc gagatcgacc agaggcggag gaagttcgcc ggttacaaga
     6001 tagatgccga tctgctcagg ctggcgaagc ccgatgcggt ggttatgcat gatctcccgg
     6061 cccatcgagg ggacgagatc actgacgatg tgatcgacgg gccccaatcc gtggtgtttg
     6121 accaggcaga gaatcgcctt cacgctcaga aggggttgct ttcctggctc ttcgagacga
     6181 agtagttcag cgtcgtaaat ggccggcagg gtcgccggcc atctacgacc tctatcgata
     6241 cgggggaccg acatgaacca gcgaaccagt gcctcctcgg gaatactctc gccggcggcg
     6301 gcggctttcc aggagaattc cagcgccttg cggatcgcca gaagcaactt tgggatggct
     6361 gggctggagg gatccagctg ctgagccctt tcgaccacct gg
//
LOCUS       DATALS010000554         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_82577,
            whole genome shotgun sequence.
ACCESSION   DATALS010000554 DATALS010000000
VERSION     DATALS010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82577"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1215)
                     /locus_tag="VHS06_12330"
     CDS             complement(88..1215)
                     /locus_tag="VHS06_12330"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX2988939.1"
                     /translation="MKQFFVSLVAVLALASLVVAGCSQAAPAAPTQAPSSSGKAAEPT
                     KATAPAAEPTKASAPAAQPTTAAAPAKKIDWPEKNKTVSLIIGYAAGGGADIAARIMG
                     PALEKELGVPVQVVNRAGAGSQVAVASVQTAKPDGYTIGYANWPNIITVYQDPERKAT
                     FTRKDFLPIAMHVADPMAIAVKADSPYKTMKDLVEAAKAKPDQIKVGHSGLLSNEHLA
                     YLKLQKEFGAKFSIVAFDGAAPEVTAVLGGHIDAAGGGFGSMLPHVKSGTMRILATFA
                     PEGKQMGINGVTTMEEQGFKGYYALSRGWFVPKDTPKEIVDALSTAMKKVILTDEQKA
                     KILESGQVTQFMDSAQMATYWEKMDTDVQPLMQLAKESDKK"
     gene            1634..1930
                     /locus_tag="VHS06_12335"
     CDS             1634..1930
                     /locus_tag="VHS06_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988940.1"
                     /translation="MIPPALESFSRMPFWGFLFVGLAAALAVFGGWSLLRRRQRQDLY
                     YFLLGLYLLITIPVLTIPAVPRELGLDAAGLGLGQRLIVALPAIALFVMAVRAR"
     gene            2143..2700
                     /locus_tag="VHS06_12340"
     CDS             2143..2700
                     /locus_tag="VHS06_12340"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX2988941.1"
                     /translation="MESSQVAALIQMTLVPVIMISAVGLVALVVQNRHAVVLERTFRI
                     NARRLDLWEELRELETTPSTARERWLRGQLDIQEHLLGLWLRRGTYTRNALVGAFLGV
                     TLFGLTSLDLVINVLMGLSGPFAQLTVAFFLAGIVAFISCFLYALMDVYQGLWITRVD
                     TKLVNDLIIRLRGGEGELMGEEREA"
     gene            complement(2794..>3293)
                     /locus_tag="VHS06_12345"
     CDS             complement(2794..>3293)
                     /locus_tag="VHS06_12345"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HEX2988942.1"
                     /translation="YLGGGFPEMHAAAIAANSPMLAAIRDAVESGMPVYAECGGLIYL
                     SQGVVDAEKKRFAMVGALPGWCSMAGKHLSMGYLEASTLKETPLGPAGTPVRGHEFHY
                     SHWDGQDDDSAAYQILNRTGRVEGYARGNLLASFLHLHFGTNPRLAPAFVESAAAYQE
                     TYSRG"
BASE COUNT          556 a          949 c         1072 g          716 t
ORIGIN      
        1 agggtagatt tccagccatt aagaagggtg gggcggctct ttcgggaacc gccccaccct
       61 tttgtgtgcg gatatgtgga acggagctta cttcttgtcg gactcttttg ccagctgcat
      121 cagcggctgg acatcggtgt ccatcttctc ccagtaggtg gccatttggg ccgaatccat
      181 gaactgagtg acctggccgg attccaggat cttggccttc tgctcgtcgg tgaggatcac
      241 cttcttcatg gccgtgctca gggcgtccac gatctccttg ggggtatcct tggggacgaa
      301 ccagccgcgg gagagggcgt aatagccttt gaaaccctgc tcctccatcg tggtgacgcc
      361 attgattccc atctgcttgc cctcaggggc gaaggtggcg aggattcgca tggtgccgct
      421 cttgacgtgt gggagcatgg atccgaagcc gccgcccgct gcgtcgatgt ggccgcccag
      481 aaccgcggtc acctcagggg ctgcgccatc gaaggcgacg atgctgaact tagctccaaa
      541 ctccttctgc agcttcaggt aggccaggtg ctcgttggag agcaatccgc tatgaccgac
      601 cttgatctga tccggcttgg ccttggccgc ctcgaccagg tccttcatcg tcttgtaggg
      661 gctgtccgcc ttgacggcga tcgccattgg gtcggcaacg tgcatggcga tgggcaggaa
      721 gtccttgcgg gtgaatgtgg ccttgcgctc agggtcctgg tagacggtga tgatgttcgg
      781 ccagttggcg tagccgatag tgtagccgtc cggtttggcg gtttgcactg aggcaaccgc
      841 aacctgggag ccggcacccg cgcggttcac tacctgcacc ggcacaccca gttccttctc
      901 cagggcggga cccatgatcc tggccgctat gtccgcgcct ccgcctgccg cgtagccgat
      961 gatgagggag acggtcttgt tcttttccgg ccaatcgatc ttcttagccg gggctgcggc
     1021 ggtagtgggc tgtgctgcgg gggcagaagc cttggtgggc tcggcggcgg gggctgttgc
     1081 cttggtgggc tcggcggctt tgcctgagct ggatggcgcc tgggtggggg ccgcgggtgc
     1141 agcctgggag catcctgcga cgacgaggct tgccagagcc aggacagcca ctagggaaac
     1201 gaagaactgt ttcattgctc ctccctaacg tctcctttgc caaatccctg ggattgtgtt
     1261 gccttgccac ggatcggggt tggcacggat cgctggtgcg atcatccggg catcagattg
     1321 aggctccaga cagaactgtg atcgtcggag gttgttggac gatctcgcga atcctttcga
     1381 ggtccctgaa atatgggggt ggaaacgcac cctcatctac cacagtcctg aagccgggtc
     1441 aacattaggg aaattatggt cagagcctga taagaagccg ggaaaggttg ccgattccgg
     1501 gtggctccgg tggggcagag aggggctcgg cccagttctt cggcggcaac ccgtataata
     1561 gtcgtaggct ggcgttgtcc ggtgagactc tctccagccg tatgacaaac caacagatgg
     1621 aggacgccgt tagatgatac ctccggcgct ggagtctttc tccaggatgc cattctgggg
     1681 ctttctcttc gtgggccttg ccgctgcact cgcagttttc gggggctgga gcctgttgcg
     1741 tcggcgccag agacaggacc tttactactt tctgctcggg ctctatctcc tgattacgat
     1801 tcctgtcctg acaatccctg ccgtgccgcg ggaattgggt ctggatgcag ccggcctcgg
     1861 gctgggacag cgactgatag tggcgcttcc ggccatcgcc ctcttcgtca tggcggtcag
     1921 ggccagatag taccttgaca ctttgacccc ggcattagga atcgcggtcg ctctggccgc
     1981 gacggttcca tgaggcagga cgatctagcc tctggagatc agacctgccg tgccggagag
     2041 ataggtgcat gcgtcacccc tgttagaaca ggcacagctc ctgcctcacc ttccgctcag
     2101 agaagtcgtg acgtaaggag ctgttgagta gctatgacgc ccatggagtc ttctcaagtc
     2161 gcagccctga tccagatgac cctggtgccc gtcatcatga tcagcgcggt gggcctggtg
     2221 gcgctggtgg tgcagaatcg ccacgcagtg gtgctggagc gaacctttcg gatcaacgcc
     2281 cggcggctgg atctctggga ggagttgcgg gagttggaga cgaccccctc gacagcccga
     2341 gagcgttggc ttcgaggaca actggacatc caggaacatc tgctggggct ctggttgcgc
     2401 aggggaacct atactcgaaa tgccctggtg ggggcctttc taggtgttac tctattcggc
     2461 ctcacctccc tcgacctggt catcaacgtg ttgatgggac tgtcggggcc atttgcccag
     2521 ctcacggtcg cgttctttct cgccggcatc gtcgccttca tctcctgctt cctctacgcg
     2581 ctgatggacg tctaccaggg gctctggatc acgcgggtcg ataccaaact ggtcaacgac
     2641 ctgataatcc ggctgcgagg cggagagggc gagctgatgg gcgaggagag ggaggcctga
     2701 ggacagcagc tgccgaagtc gtggattacc tctactgtac cgtggaacca gtggtccgcc
     2761 cctaccatcg ggcgattcgt catcgacaga gaactaaccg cgagagtagg tttcttggta
     2821 ggctgcggcg ctctccacga aggcgggagc gagccgcggg ttggttccga agtgcaggtg
     2881 gaggaaggag gcgaggaggt tgccccgggc gtagccttcg acgcgtccgg tgcggttgag
     2941 aatctggtag gcggccgagt cgtcgtcctg gccgtcccag tgggagtagt ggaactcgtg
     3001 gccccggacc ggagttcctg ccggtcctaa cggagtctcc ttcagagtgc ttgcctccag
     3061 atagcccatg ctgaggtgct tccctgccat ggagcaccag cccggcaggg ctccgaccat
     3121 cgcgaaccgc ttcttctcgg catccaccac tccctgactc aggtagatca acccgccaca
     3181 ttcggcgtag accggcatgc cggactccac ggcgtctcga attgctgcca gcatggggga
     3241 gttggcggcg atggcggccg cgtgcatttc ggggaagccc ccgccgagat aga
//
LOCUS       DATALS010000555         2954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_82655,
            whole genome shotgun sequence.
ACCESSION   DATALS010000555 DATALS010000000
VERSION     DATALS010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82655"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VHS06_12350"
     CDS             <1..188
                     /locus_tag="VHS06_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2988943.1"
                     /translation="FNIAQVPVNHYHRACGHSQFFNFSRVFRVGVDLINLWRELVLEK
                     GLSNAPTETPAIARKDR"
     gene            185..1189
                     /locus_tag="VHS06_12355"
     CDS             185..1189
                     /locus_tag="VHS06_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2988944.1"
                     /translation="MIPREECLAFYRNRSVMITGGLGFIGSNLARRLVELGADVLLVD
                     SLVPDYGGNLFNVSEIQPKLRINVADVRDESSMGYLVQRRDLIFNLAGQVSHIDSVRD
                     PYTDLEINCKAQLSILEACRKFNPRVKLVYASTRQIYGRPLNLPVDEMHPKRPTDVNG
                     INKMAGEWYHILYNSVHGIRACSLRLTNTYGPRQLMKHNRQGFIPWFIRQAMDGEEIS
                     LFGDGSQLRDFTYVDDVVDAFLMAGADPAADGEFFNVGGIGPTSLKEFLEVLLEVVGT
                     GSYRLVPFPPEKQSIDIGSFYTDYSKIKTALGWEPKVGIREGLEKTVEFYRKNREHYW
                     "
     gene            1213..2316
                     /locus_tag="VHS06_12360"
     CDS             1213..2316
                     /locus_tag="VHS06_12360"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX2988945.1"
                     /translation="MRIEANDLKPQHGEMRAEILAALERVLDDGWFVLGKQGEGFERE
                     FADFCGVAHCVGVGSGTEAIHLALLACGVGPGDDVVTVALTAVPTASAITFAGARPVF
                     VDVDPRTFTMDPSKLEDAITPRTKAILPVHLYGQTADMDPILEVASRRGIPVIEDACQ
                     GHGAEYRGRRAGSLATMGAFSFYPTKNLGACGDGGAVTTNDARLADRLRLLRNYGQRK
                     RYYHESKGFNSRLDEVQAAILRAKLRHLNAWNETRRRKAALYGSLLKGVVCPVEAEYA
                     RHVYHQYVIRTAHRDELMSYLADQGIGTLIHYPVPVHLQDAYRDLGLSRGTLPITERC
                     AGEILSLPFYPELPDAQIAEVAEAINRFGKTAL"
     gene            2375..>2954
                     /locus_tag="VHS06_12365"
     CDS             2375..>2954
                     /locus_tag="VHS06_12365"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2988946.1"
                     /translation="MEKEQYEILYRLEERHWWYLGMQRIVGSMLERYLSPAGGLRILD
                     AGCGTGGMLKYLAKFGSPVGIDVANEAVGLAHQRGLDGVARASVEELPFTASVFDLLV
                     SFDVLYHQAVVDDRRALREFHRVLKPGGLLLVRVPAYNWLRGAHDLAVHTRHRYTGSE
                     LGNKLQDAGFSLLKVTHVNSLLFPVAAAKRLME"
BASE COUNT          584 a          840 c          937 g          593 t
ORIGIN      
        1 gcttcaacat cgcccaggtt ccggtgaacc actaccatcg ggcttgcggt cactcccagt
       61 tcttcaactt ctcacgcgtt ttcagagtag gggtcgacct gatcaatctg tggcgagagt
      121 tggtgctgga gaaaggtcta agcaatgcgc cgactgaaac cccggcgatt gcgcgaaaag
      181 accgatgatc ccccgcgagg agtgcctcgc tttctaccgc aaccgatcgg taatgatcac
      241 cgggggcctc ggcttcatcg gcagtaacct cgcccgcagg ctcgtagaac tgggcgccga
      301 cgtcctctta gtggattccc tggtacccga ctacggggga aacctgttca acgtttcaga
      361 gatccaaccc aagctgagga tcaacgtcgc agacgtccgt gacgagagca gcatgggcta
      421 cctggttcaa cggcgtgatc tgatcttcaa cctggcgggc caggtcagcc acatcgatag
      481 tgtccgtgat ccctataccg acctcgagat caactgcaag gcccaactct ccatcctcga
      541 agcctgccgc aagttcaatc ccagggtgaa gctggtatac gccagcactc gccagatcta
      601 tggaagacct ctgaacctcc ccgtggatga gatgcacccc aaacgtccga cggacgtcaa
      661 tgggatcaac aagatggccg gcgagtggta tcacatcctg tacaacagcg ttcacgggat
      721 tcgtgcctgc tcgcttcgac tgaccaacac ctatggccct cggcaactca tgaagcataa
      781 tcgacagggc ttcatcccct ggttcatccg gcaggcgatg gatggggagg agatctcgct
      841 cttcggcgac ggcagccagc tgcgagattt tacctatgtc gacgatgtcg tggatgcctt
      901 cctgatggcc ggagccgatc ccgctgccga cggggagttc ttcaacgtcg ggggcatcgg
      961 gcccaccagc ctgaaggagt tcctggaggt gttgctggag gttgtcggta ccggcagcta
     1021 tcggctggtg ccgttcccgc ccgagaagca gtccatcgac atcggcagct tctacaccga
     1081 ctactccaag atcaagacgg ccctggggtg ggaacccaag gtgggtatcc gggaggggct
     1141 ggagaagacc gtggagttct acaggaagaa ccgagagcac tactggtaga ttagtaaggt
     1201 ggatggtggg ctatgcggat agaggcaaac gacctgaaac cccagcacgg agagatgcgg
     1261 gccgagattc tggctgcgct ggagcgggtg ctggatgatg gctggttcgt tctgggaaag
     1321 cagggagagg gcttcgagcg ggagttcgcc gacttctgcg gcgttgctca ctgcgtaggc
     1381 gtcggctccg gcacggaagc gatccatctg gcgttgcttg cctgcggtgt tgggccgggc
     1441 gatgacgtcg tcacggtcgc cctgaccgcc gtgcccacgg cctctgccat caccttcgcg
     1501 ggtgcgcggc cggtgttcgt ggatgtggat cctcgaactt tcaccatgga cccctccaaa
     1561 ctggaggatg cgatcacgcc tcgaacgaag gcgatcctcc cggtgcacct gtacggccag
     1621 actgcggaca tggatccgat cctggaagtg gcttcgcgcc gggggatccc ggtgatcgag
     1681 gatgcatgtc agggccatgg cgcggagtat agggggcgcc gggccggctc cctggctacg
     1741 atgggcgcct tcagcttcta tccaacgaag aacctcggag cctgtggtga tggtggcgcg
     1801 gtaaccacca acgatgcgag gcttgcggac aggctgcggc tgttgcgcaa ctacgggcag
     1861 cgaaagcgct actaccacga gagcaagggg ttcaacagcc gtctggacga ggtgcaagct
     1921 gcgatacttc gggcgaagct gcgtcacctc aacgcgtgga acgagactcg gcggaggaag
     1981 gcagcgctgt acggctccct gctgaaggga gtcgtttgcc ctgtggaagc agagtatgcc
     2041 cggcacgtct atcaccagta tgtgattcgc actgcccatc gcgacgagtt gatgagttac
     2101 cttgccgacc agggcatagg cacgttgatc cattaccccg tgccggttca cctacaggat
     2161 gcgtataggg atctggggct ctctcgaggg acgcttccta ttacggagcg ctgtgcggga
     2221 gagatcctgt cgttaccctt ctacccggag cttccggacg cgcagattgc ggaggttgcg
     2281 gaggcgatca accgcttcgg caagacagca ctgtaagggc gggtctaaga cccgccctta
     2341 cttacaagaa gacatatctc aagaagacat atctatggaa aaagaacaat acgagatcct
     2401 ctatcgcctg gaggagagac actggtggta tctgggcatg cagaggatag tgggctctat
     2461 gctggagcgt tacctgagcc cggccggcgg gctgcgcatt ctagatgcgg gatgcgggac
     2521 cggcggcatg ctgaagtatc tggctaagtt cggttcgcct gttgggatcg atgtggccaa
     2581 tgaggctgta ggcctggccc atcagcgtgg gctggatggc gttgcccgcg catcggtgga
     2641 ggagctgccc tttaccgctt ctgtcttcga cctgttggtg tctttcgacg tcctgtacca
     2701 ccaggcggta gtcgacgacc ggagggcact gcgggagttt cacagggtgc tcaagccggg
     2761 cggactgcta ttggtgcggg tgccggccta caactggctc cgcggggctc acgacctggc
     2821 cgtccacact cgccatcgat acaccggttc agagttgggc aataagctgc aggatgccgg
     2881 tttcagcttg ctcaaggtga cccacgtcaa ctctctcctt ttccccgttg ccgctgctaa
     2941 gcggttgatg gaag
//
LOCUS       DATALS010000556         6221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_82902,
            whole genome shotgun sequence.
ACCESSION   DATALS010000556 DATALS010000000
VERSION     DATALS010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82902"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..673
                     /locus_tag="VHS06_12370"
     CDS             290..673
                     /locus_tag="VHS06_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2988947.1"
                     /translation="MEEEQGLMTMKVLVVDDDEAIGEFVDWVLTDEGHEVTKAGNGAE
                     ALQLLEELRPDVILLDMRMPVMDGWQFADAYRKGNYPRAPIVVVTAARDATERAEEIG
                     ADGYLGKPFDIDDLLATVTRFRSDD"
     gene            712..2238
                     /locus_tag="VHS06_12375"
     CDS             712..2238
                     /locus_tag="VHS06_12375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX2988948.1"
                     /translation="MLVDTHAHLTTAELAADLPGVLARAVAAGVDAVVCVGIELKSSR
                     EAILLAHHHPRLRATVGVHPNDCSELGPSWLDELRSLARASEVVAIGEIGLDYYRQST
                     GHELQRELFRQQLDLAGDLGLPVVVHNREADEDVASILVKWSAGLAEGHPRGVLHCFS
                     GDRDLMDAVVAAGFYVSFAGPITYQNGRKAAEMAASAPSDRVLVETDSPYLAPHPHRG
                     KLNEPALVRLVVERLAELRSESVEEAIENTGRNARPNLHLGMASSVGRESRMSNIRTK
                     PRVLVVDDNLQTLKLLRFLLEDEGYEVIVASNVRLARSILEGPPGPISLVMLDVMMPG
                     VDGFEFCQELRGAGNDMPIIFLSARGDHEAKAEGLRMGADDFVAKPFDPTELLARVGA
                     VLRRARPSGGVGESQPVLRLGEVELDAARLKVRTGPGRESDLTPTETKLLHCLMRNAG
                     RVLTRDALISQVWDYDYDGNNNTVDVYIRRLRQKVEPDPNDPRYIRTVRGAGYIFQAA
                     "
     gene            2472..4028
                     /gene="guaA"
                     /locus_tag="VHS06_12380"
     CDS             2472..4028
                     /gene="guaA"
                     /locus_tag="VHS06_12380"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX2988949.1"
                     /translation="MLPPPDVREAVAVIDFGSQYSQLIARRVRECNVYCELIPYNAGP
                     ERIAALNPRGFILSGGPSSVYQEGAPRLSDFILKSGLPILGICYGMQLLAHQLGGEVA
                     PGTRREYGYAEVAVGEPVAPIFREMPASFQVWMSHGDVITRMPPGFRALARTENSPLA
                     AMGNDAGLTGLQFHPEVVHTPLGKEIIRNFLYNECGCTGSWTPGSYIAETTERIRQQV
                     GEGRVICALSGGVDSAVAATLIQHAIGDRLTCVFVNNGVLRLNEAEKALETFRRNLRM
                     NVVYVDATDRFLSHLDGVIDPEQKRRIIGGEFIRIFEEEAAKLGKIDFLAQGTLYPDV
                     IESTSHDTNAAARIKTHHNVGGLPEEMQFALVEPLRYLFKDEVRRVGLELGLPEEMVW
                     RQPFPGPGLAVRIIGAVTPGRIETLQLADAVVTDEIRKAGLYRQVWQSFAVLTPLQTV
                     GVMGDGRTYANVVAVRIVNSDDGMTADWARVPYDVLARISSRIVNEVPGVNRVVYDIS
                     SKPPSTIEWE"
     gene            4114..5511
                     /gene="purD"
                     /locus_tag="VHS06_12385"
     CDS             4114..5511
                     /gene="purD"
                     /locus_tag="VHS06_12385"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX2988950.1"
                     /translation="MKVLVVGGGSREHTLVWKMLLSPQVDQVYCAPGNAGTGILAYNV
                     PIAATNVAALTQWAVANKIDLTIVGPERPLVEGIVDSFQDAGLRVFGPAAKAAAIEGS
                     KAWTKDLLVRHGIPTPRAEVLTEYGKAEEALDRCAFPIVVKADGDAAGKGVVIAQDRD
                     SALAAVKSFMADRTLGSAGDRVLLEEFLEGVELSVLAFTDGKTIVPMAPACDYKRVRD
                     NDAGPNTGGMGAYARPAFATPELMETIQRTILDPTISALAEEGCPYSGALYAGLMITR
                     EGPMVLEYNCRFGDPEAQVVLPLLKSDLVDIVQAVIDGRLAEQPIEWGDEACCGVVLA
                     SEGYPGEYETGQPISGLDDLEDGVLVFHGGTTMGRPARKDPFASIKKELMIRDWGPPV
                     LITAGGRVLTVAACGATMAEARAKVYRNAERIDFPGRHFRHDIAARELSSISHQSSAG
                     EEQASDRTSGADYAI"
     gene            5691..6152
                     /gene="purE"
                     /locus_tag="VHS06_12390"
     CDS             5691..6152
                     /gene="purE"
                     /locus_tag="VHS06_12390"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX2988951.1"
                     /translation="MPQVAIIMGSESDKPTMQLAADVLDSLGVSFEVVVMSAHRTPEK
                     VREYAKAARENGVKLIIAGAGMAAHLPGVIAAWTSLPVIGVPLPAGELRGQDALYSMC
                     QMPSGVPVATFGIGNSGARNAAYFAASVLALSDPVVRTSYDEFRKRQSGES"
BASE COUNT         1181 a         1857 c         2114 g         1069 t
ORIGIN      
        1 gtcgccacct tccctggtgg agttggctcg ccggcttcgc tccgagggcg ttcggggtaa
       61 agacgcggtt cgcaggctgg tggacgaaac gggagccggg cgccgcgatg cctatcaggc
      121 atggcttgat gccggcgccc agacagatcc atgaggtgct tttcgctcgt tcttggcggc
      181 atgacaattg cagccactcc gtctcttgaa ggaccctcag ttcaatctat caatttcgcg
      241 tgtccacact aaacaagact caacacactg ggaagtcatg cgccaactca tggaagaaga
      301 gcagggactt atgacgatga aggtgctggt cgttgacgat gacgaagcca tcggcgagtt
      361 cgttgactgg gtactcactg acgagggaca tgaggtcacg aaggccggca acggagctga
      421 agcactccaa ttgctggagg agctacggcc agacgtgatc ttgcttgaca tgcggatgcc
      481 ggtaatggat ggatggcaat tcgcggacgc ctaccggaaa ggcaactacc caagggcgcc
      541 gatcgtggtg gtgaccgccg ccagggatgc cacggagcga gcggaagaaa tcggtgccga
      601 tgggtacctc ggaaagccgt tcgatatcga cgatctgctc gccactgtca ctcgctttcg
      661 ctcggacgat tagtcgactc ccgtaaatgc cttgtgctac aatccggcca tatgctggtc
      721 gatacgcatg cccacctcac gacggcggag ttggcggccg atctgccggg tgttcttgcc
      781 cgcgcggtgg ccgcgggcgt cgatgccgtc gtctgcgtgg gcattgaact gaagtccagc
      841 cgagaggcca tccttctcgc ccaccatcac ccacggctcc gtgctacggt cggcgtgcat
      901 ccaaacgact gctccgagct gggcccgagt tggctggacg agctgcggag tctggcccgc
      961 gcatctgagg tcgtggccat cggcgagatt gggctcgatt attatcgcca gagcaccgga
     1021 cacgagctac agagggagtt gttccggcag cagctggacc tggcgggcga tctcggcctt
     1081 ccggtagtgg tccacaaccg tgaagcggac gaagacgtcg cgtccatcct ggtaaaatgg
     1141 tcggcgggac ttgccgaagg gcatccgagg ggggtgctcc actgcttctc gggggatcgg
     1201 gacctgatgg atgcggtggt ggcggccggg ttttacgttt ccttcgcggg gccgattacc
     1261 tatcagaacg gcaggaaagc cgcggagatg gcggcttctg ctccctcgga ccgcgtactg
     1321 gtagagactg attcgcctta cctggcacct catccccaca gaggcaagct caacgaaccg
     1381 gcgctggtcc ggcttgtggt tgagcgattg gcagagctac ggagcgagtc cgtagaagaa
     1441 gctatcgaga ataccgggcg caacgcgagg ccgaatcttc atctaggcat ggccagttcg
     1501 gttggccggg agagtaggat gagcaacata cggacgaagc ctcgggtgct ggtggtggac
     1561 gacaacctgc agaccttgaa gctgctgcgg ttcctcctgg aggacgaggg gtacgaggtg
     1621 atcgtggcga gcaacgtccg cctggcccgc tccatcctgg agggcccgcc ggggccgatc
     1681 agcctggtga tgctggacgt gatgatgcct ggggtggacg gcttcgagtt ctgccaggag
     1741 ctgcgcggtg cggggaacga catgccgatc atcttcctgt cggcgagggg ggaccacgag
     1801 gccaaggccg aggggctgag gatgggggcg gatgacttcg tggccaagcc cttcgacccg
     1861 accgagctct tggctcgggt gggggcggtg ctgaggaggg ccaggccctc gggaggggtg
     1921 ggggagtccc agcctgtcct taggctgggg gaggtggagc tggacgcggc gcgcctgaag
     1981 gtgaggacgg ggccagggag ggagagcgac ctcacgccca ccgagaccaa gctcttgcac
     2041 tgcctgatga ggaacgcggg gcgggtgctg acgagggacg cgctgatcag ccaggtgtgg
     2101 gactacgact acgacgggaa caacaacacg gtggacgtgt acatcaggcg gcttcggcag
     2161 aaggtagagc cagatcccaa cgacccgcgc tatatcagga cggtgagggg ggcgggctac
     2221 atcttccagg cagcctagac catagggcga agtgagaatg gtgaatggtg aatggcgact
     2281 gcttcaccct tcaccattct taattcaccc ttcgaaggac ctgctcgcct tgactttacg
     2341 gctgttcggt tgatatgctc cgggtcaacc gggggctcct tttctgccgc cccgtccccg
     2401 aaatgtgcct gccggtatcg aggaccgcag agtaggagga acctaccacg agcgctcagc
     2461 aggaagcccc gattctaccg cctccggacg tccgcgaagc ggtggccgtc atcgatttcg
     2521 gatcccagta cagccagctc atcgcgcgga gagtccgcga gtgcaacgtt tactgcgagc
     2581 tgatccccta caacgcgggc cctgagcgga tcgccgcgct caaccccagg gggttcattc
     2641 tctccggcgg gccctcctcc gtgtatcagg agggcgcgcc aaggctctcg gatttcatcc
     2701 tcaagagcgg actgccaatc ctagggatct gctacggtat gcagctcctt gcccaccagc
     2761 tcggcggaga ggtggcgccg gggactcgcc gggagtacgg ctacgctgag gtggccgtgg
     2821 gcgagccagt ggcgccgatc ttccgcgaaa tgcctgcctc gttccaggtc tggatgagcc
     2881 acggggacgt cataacccgc atgcccccgg gcttccgggc actcgcgcgt accgagaact
     2941 ccccgctcgc cgcgatgggc aacgacgccg ggttgacggg cctccagttc caccccgaag
     3001 tggtccacac gccgctcggc aaggagatta tccgcaactt cctgtacaac gagtgcggct
     3061 gcaccggaag ctggacgccc ggctcttaca ttgccgagac gaccgagcgg atcaggcagc
     3121 aggtcggcga aggccgcgtc atctgtgccc tctcaggggg cgtggactcg gcggtggcgg
     3181 ccacgctgat ccagcacgcg atcggagacc gcctcacctg cgtcttcgtg aacaacggcg
     3241 tcctcagact caacgaggcc gagaaggcgc tggagacatt ccggcggaat ctcaggatga
     3301 acgtcgtgta cgtggacgcg accgaccgct tcctctccca ccttgacggc gtgatcgatc
     3361 cggagcaaaa gcgacggatt atcggcgggg aattcatccg gattttcgag gaggaagcgg
     3421 ctaagctcgg caagatcgac ttcctggcgc agggaacgct ctaccccgac gtgatcgaga
     3481 gtacgtctca cgacaccaac gcggccgcgc gcataaagac ccaccacaac gtgggcggcc
     3541 tgccagaaga gatgcagttc gcgctggtgg aaccgctgcg ctacctgttc aaggacgaag
     3601 tgcgacgcgt gggcctggag cttggtctgc cggaggagat ggtgtggcgc cagcccttcc
     3661 cggggcccgg cctcgccgtc aggatcatcg gggccgtgac gccggggagg atcgagaccc
     3721 tgcaacttgc cgacgcggtg gtcacggacg agattcgcaa ggccggtctc taccgccagg
     3781 tgtggcagag ctttgcggtg ctgaccccgc ttcagacagt gggcgttatg ggggatggac
     3841 ggacttacgc gaacgtcgtg gcggtgcgca tcgtgaacag cgacgacggc atgacagccg
     3901 actgggctcg cgttccctac gacgtgctgg cccgcatctc cagccgcatc gtcaacgagg
     3961 tcccaggcgt gaaccgcgtg gtttacgaca tcagctcgaa gcccccatcg accatcgagt
     4021 gggaatgacg gaagagtgaa tggtgaatag tgaagatggc tcctttcacc cttcaccctt
     4081 ccgacttcac tcttctttgt gaggaatgag ttcatgaaag tgctggttgt cggcggtggt
     4141 tccagggagc acaccctggt ctggaagatg ttgctcagcc cccaggtgga ccaggtgtac
     4201 tgtgcccccg gcaacgcggg cacgggcatc ctcgcctaca acgttcccat cgccgccacc
     4261 aatgtggccg ccctcaccca gtgggcggtc gcgaataaga tcgacctgac tatcgtcggc
     4321 ccagaacgac cgctcgtcga ggggatagtg gattcgttcc aggacgctgg cctccgcgtc
     4381 ttcggtcccg cggcgaaggc cgcggccatc gagggaagca aggcgtggac gaaggatctg
     4441 ctggtcaggc acggcatccc gacgccgagg gcggaagtgc taacggagta cgggaaggca
     4501 gaggaagcac tggaccggtg cgcgttcccc atagtcgtaa aggcagacgg tgatgccgcc
     4561 ggcaaggggg tcgtcatcgc gcaggacaga gacagcgctc tagcggctgt aaagagcttc
     4621 atggcggacc gcacccttgg atcagctggc gacagggtgc tcctggagga gttcctcgag
     4681 ggcgttgagc tctcggtcct cgccttcacc gacggcaaga ccatcgtacc gatggctcca
     4741 gcctgtgact acaagcgggt gcgggacaac gacgcagggc caaacacggg cggcatgggc
     4801 gcatatgcaa ggccagcgtt cgccacgccc gagttgatgg agacgatcca gaggaccatt
     4861 ctcgacccga ccatctccgc gttggccgag gagggatgcc cctatagcgg cgcgctgtac
     4921 gcagggttga tgatcacgcg cgaaggcccg atggtgctgg aatacaactg ccgcttcggc
     4981 gacccggagg ctcaggtggt gctcccactt ctgaagagcg accttgtgga catcgtccag
     5041 gcggtgatcg acggacggct cgccgagcag ccgatcgagt ggggcgacga agcctgctgt
     5101 ggcgtcgtcc tcgcctcgga gggctaccca ggcgagtacg agacagggca gccaataagt
     5161 ggcctcgacg acctggagga cggcgtcctc gtcttccacg gcggcacgac gatgggccgc
     5221 ccggcaagaa aggacccatt cgcgagcatc aagaaggaac tgatgatacg agactggggt
     5281 ccccccgtcc ttatcaccgc cggcggcagg gtgctcacgg ttgccgcgtg cggggcgacg
     5341 atggccgagg cgagggccaa ggtctaccgc aacgccgagc ggatcgactt tccggggcgc
     5401 cacttccgcc acgatatagc ggcgagggag ttatcatcta tcagtcatca gtcatcagcg
     5461 ggcgaggagc aagcaagcga tcggacgtcc ggcgccgact acgctattta ggcgggtcca
     5521 gggcgcactt gacggtagcg agtacttcac gcgagcgagc gcagcgtgaa ggcccgaggg
     5581 ccgtgccgtc aaggccgaag gccgcctggc ggggttcggg gtgtccccga agaaacacct
     5641 cttttttccc ccttcctggg gaagggggtc ggggggaagg aggtcagtcc atgcctcagg
     5701 tggccataat catgggcagc gagagcgaca aacccacaat gcagctcgcg gcggacgtcc
     5761 tggattccct gggggtatca ttcgaagtcg tggtgatgtc cgcgcaccgc acaccggaaa
     5821 aggtgcgtga atacgcgaag gcagccaggg agaatggcgt caagctgatc atcgccggag
     5881 ccggcatggc ggcccacctt cccggcgtga tcgcggcctg gacgtcgcta ccggtgatcg
     5941 gggtgccgct gccggcgggc gagctacggg ggcaggacgc cctttattcc atgtgccaga
     6001 tgccctccgg agtgccggtg gcgacgttcg ggatcggcaa cagcggcgcc cgcaacgccg
     6061 cctacttcgc ggcctcagtg ctcgctctct ccgacccggt ggtacgcacc agctacgacg
     6121 agttccgcaa gcgccagtca ggagaatcgt aaggggacag gggacagggg tcggggtcag
     6181 ggtcgggagc tacaacctcc gaccccggtc cccagtaccc c
//
LOCUS       DATALS010000557        12190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_83177,
            whole genome shotgun sequence.
ACCESSION   DATALS010000557 DATALS010000000
VERSION     DATALS010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12190
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83177"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="VHS06_12395"
     CDS             complement(<1..1629)
                     /locus_tag="VHS06_12395"
                     /inference="COORDINATES: protein motif:HMM:NF039430.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector-associated domain EAD1-containing
                     protein"
                     /protein_id="HEX2988952.1"
                     /translation="MTASARLSGADCERLNEVIARAFPTRAELAQLVRYKLDENLQAI
                     AGGDDLAEIVFNLIGWAEAKGRVGELIEKGLEMRPGNADLEACANQLRTVASQSPALQ
                     GLLDLSQLLMRSDELVHTQIQQFARSKYISGLYVRRDIQDQLGRFHTSERELARDLKT
                     LQVKTIARWAAALSDAVSFFSGICELVSTITDWEDTESSSTIHAHRAARKMNLDGDES
                     HNRVPSRSQALEATTTAKEIVQAISASLETTLHHVQALSDERILGETAAHQSVVQALG
                     VAMATLPKLMESAEDADAQSVMLISVLDEPLRLTGQLMRSLPHRPGILARYAAEDLPK
                     QWPQVFKTLSGQSLATLASELNSSIAAFESAQRHAVIIIDRAGGGKTNLLCELALQTV
                     KVSPTLLFFGKDNFSDHGSMIRRTTDWVAGMLSTHPDTAMMILDQLLQDRGLFLHVFI
                     DGINESRSIPAMDTALASFLEWSSHHRLRVSISCRDIYWDFFDYDKWSQYVHHTIHNK
                     LHQFTPSESARARDLYFAHFGIKCTLVGNAETACR"
     gene            complement(1689..2786)
                     /locus_tag="VHS06_12400"
     CDS             complement(1689..2786)
                     /locus_tag="VHS06_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015119026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector-associated domain EAD1-containing
                     protein"
                     /protein_id="HEX2988953.1"
                     /translation="MRLNGRQYERLVSALVDAYPTVFRLRQMLRYRLDKNLDTIALGD
                     DLLEIAFKLVQAGEAEGWTSNLVLAARESNTGSPDLLALAEELGLASSSVSNNAIESI
                     IRASNSFLDVEVWRERLGQVETQVCRVELPTNRGHIYGTGFLIAPDLVITNYHVMEAA
                     IRCTQKQRASEKAEANPADVVLRFDFRRLGDGTVLDPGTEYRLPPSDWLLDYSPRSTV
                     QDNPPIDELDYAIVRVAGSPGLDSVGKGAEPEADSRGWIRLRPDHHHFRPGTPLLIMQ
                     HPNSAPLKLSIDTDAIVGVNTQRTLVTYRANTLPGSSGSPCFDINWNLVAVHHSGDPG
                     FLHTGLNSGTPTDAIWRLLDKRGIAAPLPSD"
     gene            complement(3070..3900)
                     /locus_tag="VHS06_12405"
     CDS             complement(3070..3900)
                     /locus_tag="VHS06_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988954.1"
                     /translation="MATSRVDYTAQAVEASRSVLLELCHLLGEYRDDIVVIGGWVPEL
                     LISRGDIHHVGSMDVDLALNHRNLQEAGYRTIQELLLFRGYRQGNQPFVFHRLVRVGD
                     HEVSVEVDLLAGEYQGTGSGHRTQKVLDVRARKARGADLAFEMVRNVEISGTLPGGGK
                     DTADVRVAAIVPFLVMKGMALHDRMKEKDAWDIYFCLRYYPGGLDALVKEFQPHLDHG
                     LVREGLSKIAEKFASPEHVGPRFVADFDGLDDPEERALRERDAYERVSLLLQQLKVAH
                     "
     gene            complement(3882..4805)
                     /locus_tag="VHS06_12410"
     CDS             complement(3882..4805)
                     /locus_tag="VHS06_12410"
                     /inference="COORDINATES: protein motif:HMM:NF021469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HEX2988955.1"
                     /translation="MPKGKQLLVVEVKGTGQPRLAREAINQLLRYIQLKPNAYGIFVA
                     PYISPESAEICRQEGVGYLDLAGNCYLAFGQVFIRRECRPNPFVQRRDLRTLYSPKAE
                     RVLRVLLQRPNRAWKTEALANEARVSLGQVANVKRLLADREWIQVGVDGFRLSKPERV
                     LAEWTGNYGLRRNQTVDFYTAKEISEVEYELAGVGTSDETRSVLTGFSGAARFAPFVR
                     YSRVTAYVPDSVDRIASRLGLKQVPSGSNVNLVVPYDEGVFYGAEDVRGTRVASPIQL
                     YLDLRTMKGRSEEAANALLEEVIKPRWQQAE"
     gene            5353..5808
                     /locus_tag="VHS06_12415"
     CDS             5353..5808
                     /locus_tag="VHS06_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988956.1"
                     /translation="MTPIVVEVGCKTAGVAVGVHDIGPVALQLMEERLTVAELIRRTV
                     EEQVRELLVRRKLAADQARQLLDRQYLTEKEIARQAEHGAVRYPSGRVRQEPPIDPAA
                     EVWKALRGFENGSYLILIDGRRMERLEETIDFAPGVQVTFLRVMPLMGG"
     gene            5841..8783
                     /locus_tag="VHS06_12420"
     CDS             5841..8783
                     /locus_tag="VHS06_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HEX2988957.1"
                     /translation="MFRLMLRGDRDGRLEEQIRLCIERYWDFQELEAWCKTNRWDLPV
                     AALDTAYPPLTHSLDHLRARWSEDKLSRLAGDMYFNPARWQHSYMTDWTQRWVMQLVL
                     VAILLRFPPPDLFTRIEQRMKLIFEHPLDGEEIPAPFRQRSWPPDLYLSPALEDCLKL
                     LRALPATAEKIALVEKVALGELDRGPFAVPAYSSSDRDSMRRAYDIYVGSLVYRASWM
                     WEFLYDRGRFGYESFCRATEHLPTLLSTLSMEHTLYGGLTPFYAGVSEAFSATLKGYC
                     DRLAWELAQDISEATCKRLIEIHELRGSRYLVEAGRQHSRHGLGRLIYKAFEYPETVQ
                     SAIVHIARAADTEPSLSEDRQRVVKELRALPIDTLKYLLPIAPGSRHLLCEALGWQAA
                     APLLEQFVLIGRPDRQGGFSPVGSGNPEDPSVGVLDVPTVREALGVAGEPLTREVLSL
                     FRKAKVGAEDVVMLIEAVAGWDRPQVEKSFTKRKQAAVKAYGLLPLERGEDEALDRYL
                     SLQAFARESKKLGSERQANETAAVQAALANLAQVAGFPDAVRLEWAMEGRLSTTVAAP
                     DRTWMVDDYRIELRLDVDRPELAFWRGDRPLKSPSEAVRRSEVYAEAKEAVAQLRAQI
                     ARFRHALENMLAVGDALSHDDLEMLLRLPVLGAMLPRLVLRGDDGIFGLFDPKDHVLI
                     PLKGGPRSLAHPARIAHPYDLLLAGQTSAWQREIVRRRIVQPFKQLFREVYLLTPAEA
                     ETVNYSNRFAGHVVDGRVASRLLQARGWQIETGDIALPYKLFRKCGLASFFVFPDAGH
                     YLGELDTVTSDLVYFQPIDARDRWQGWRDETRVPLADVPPIVFSEVMRDADLVVSVAQ
                     RGEDGLLSEEAYQQRAAVVTSLLEDLRLPGVTVEGHFAHVEGKLARYRVHLGSAVVHI
                     EPGNYLCVVPAKRGNHPERLFLPFADDGDSKITEVISKILLLVSDDRIKDDSILRQIR
                     VARSPQDG"
     gene            9101..9565
                     /locus_tag="VHS06_12425"
     CDS             9101..9565
                     /locus_tag="VHS06_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988958.1"
                     /translation="MIAFASCAAWALTRIRETCQCGREIEDSPKSRLAAVLSAARPVI
                     LALLASLAAAAPQPPYFQPFDVETALQPNGDFVVIESQTAVFGLQSARHGFRVIPMDR
                     AEQGDYFKKRGWIRAFATTLSTSATSSHAEGTSTLGTAEGPGSEPTLDPPSP"
     gene            complement(9894..10412)
                     /locus_tag="VHS06_12430"
     CDS             complement(9894..10412)
                     /locus_tag="VHS06_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988959.1"
                     /translation="MIQNPEAGENVPQVVFVFPSIRFVASRVRYSEDLLAMLPPLAAA
                     ARLVPEGLPPRGPNTGPLCTLSFEGAGALERDEPPLRRGGGVGVAVGASVAVFVGVGE
                     LVTRAVAVGVVGEPVGDVVDVLVGDAEGEVGAAICVGVDVLLRVAVAVFVEVAALVGV
                     AVGLLSSASTAA"
     gene            10351..>12190
                     /locus_tag="VHS06_12435"
     CDS             10351..>12190
                     /locus_tag="VHS06_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX2988960.1"
                     /translation="MDGNTNTTWGTFSPASGFWIIVDLGSIYTIGSIRVNTSSAGDPD
                     GAGSCYPRSINVLTSTTSTDPVSGFTLAGSFEATQNDWTDYSFSPVAARYVLLSIQTI
                     WPGGGENAADVAELEVFTPNAAPTISGTAPHSFGDEHLVNPAQVSAWMAGLTTGDTDG
                     NTVGIAITGTTGNGTWRYSIDSQATYTALPAVSDTSALLLRPSDWISYTPDLQNGETI
                     TLTVRGWDQTTGSAGSSADISINGGWTAFSTGTATISSTIAAINDAPTLSGGPFILTG
                     TNEDTASSGTLISTILAGLTYADVDSGAQSGIAVFGKTGNGTWQYSTDGTSGWTSIGS
                     PTVNSGVLLASSTYIRYVPNGHNGETATLSFSGWDRTTGAPSANGSPATADPTPGGGT
                     TAFSTGTAQASITVASVNDAPIIGSGTSTLTATIANVASTPKMVNQMFSDFGYTDVDL
                     AAQPGIAVTAKTGLGTWEYSVDAVTWTSFGAVSDSAALLLDSDSFVRYTGDGSSGETA
                     TFTFRAWDRTSGTASTYGTPCTADTTTNGGTSAFSTGTAMAQISVSALNHAPTMSGGG
                     HHSREHERGYRWLQPGGVRVPDELRSERQRRRHDGDRRHRHGGQRHL"
BASE COUNT         2413 a         3667 c         3599 g         2511 t
ORIGIN      
        1 cctacacgcg gtttcggcgt ttccgacaag agtgcacttg atcccaaagt gtgcgaaata
       61 caaatccctg gctcgagcgg actctgatgg agtgaactga tgcagcttgt tgtggatagt
      121 gtgatgaaca tactgagacc acttatcgta gtcaaagaag tcccaataga tgtccctgca
      181 gctgatggat acccggaggc ggtgatgact tgaccactca aggaagctgg ctaacgctgt
      241 atccattgct ggtatgctgc gagactcgtt tatgccgtca ataaacacgt gtaagaacag
      301 accccggtct tgcagtagct gatcaagaat catcatagcc gtatccggat gcgttgataa
      361 catgccggca acccagtctg tcgttcttcg gatcatgctg ccatgatcgg aaaaattgtc
      421 ctttccgaag aagaggagcg taggagagac cttgacagtc tggagagcca gttcgcacag
      481 gagattggtc ttccctccgc ccgctcggtc aataatgatg acggcatggc gttgcgcact
      541 ctcaaaggcc gcaatggatg agtttagttc tgatgccaga gtggcgaggc tttgaccaga
      601 gagtgtcttg aagacctgag gccattgttt tggtaggtcc tcggcggcat atcgggccag
      661 gatacctggt cggtgaggta ggctgcgcat cagttggccg gtgagccgca gtggctcgtc
      721 gagcacactg atgagcatca cgctttgcgc gtcggcgtct tcagcgcttt ccataagctt
      781 ggggagcgtt gccatagcaa ctccaagggc ttgtacgacg ctctgatggg cggctgtctc
      841 accgagaatc ctctcatctg acagggcctg aacgtgatgg agtgtcgtct ccaggcttgc
      901 tgagatggcc tgcacgatct cctttgccgt agtagtggct tcaagcgctt gggatcgact
      961 aggaactctg ttgtgacttt catccccgtc caggttcatc ttgcgtgcag cacggtgcgc
     1021 atggatcgtc gatgacgact cggtgtcctc ccagtcagtg atggtggaga caagctcgca
     1081 tatgccgctg aagaacgata cggcatccga gagggcggca gcccacctgg caatggtttt
     1141 cacctggagg gtcttcaggt cccgtgctag ctcgcgctca ctagtgtgga atcggccaag
     1201 ttgatcttga atgtcccggc gaacgtagag gccactgatg tacttgcttc tggcgaattg
     1261 ttgaatctgg gtatgaacca gttcgtcgct ccgcataagc aactgggata gatccaagag
     1321 cccttggaga gcaggcgatt gtgaggcgac agttcgcagt tgatttgcac aagcttctag
     1381 gtcggcgttc ccaggcctca tttcgaggcc tttctctatg agttcgccaa ctcggccttt
     1441 cgcttcggcc cagccaatca gattgaagac gatttctgcc aggtcgtctc caccagcaat
     1501 tgcctgcaga ttctcgtcga gcttgtagcg cactaactga gcaagttcgg cccgagttgg
     1561 aaaagctctt gcgataacct cgttcagcct ttcgcagtca gcaccactca gccgtgcaga
     1621 agctgtcata gaccatgctc cttcatgggg cacgtgggaa cgacacttgt ctaacctacc
     1681 tccgccgcct agtcggatgg tagtggtgcc gcaatgccgc gtttgtccaa tagtctccag
     1741 attgcgtctg taggtgttcc agagttcagc ccggtgtgca gaaaacctgg atcgccactg
     1801 tggtggactg caaccaggtt ccagttgata tcgaaacaag gagagcccga cgatcctgga
     1861 agcgtgtttg cccggtaagt gacgagtgtt ctctgggtgt tgacccccac gatggcgtca
     1921 gtatcgatgg acaacttcag gggggcactg tttgggtgct gcatgattaa gagcggtgtg
     1981 ccaggccgga aatgatgatg gtctggacgc agacggatcc aaccccgcga atccgcctca
     2041 ggctctgcgc ccttcccaac gctgtcgagc cctggcgagc cagcaacgcg cactattgca
     2101 tagtccagct cgtcaatcgg agggttgtct tggacagttg atcgtgggct gtagtcgagc
     2161 agccaatcgc ttgggggcaa acgatactcg gtccctgggt ctagtacggt cccatcacct
     2221 agtctccgaa aatcgaacct caggacaaca tctgcaggat tcgcttctgc cttttcggat
     2281 gctcgctgtt tctgcgtgca acgaattgct gcttccataa catgataatt ggtaatgacc
     2341 aagtcaggcg ctatcagaaa ccctgtcccg tagatatggc ctcggttggt gggcaattcg
     2401 acacggcaga cttgcgtctc tacctgacct agtctctcac gccagacttc cacgtctaaa
     2461 aaactgttag atgctctgat gatgctctca atcgcattgt tggagacact ggatgatgcc
     2521 agtccaagtt cttcggccag ggcaagtaga tctgggctgc cggtgttcga ctcacgagcc
     2581 gccaggacaa gattggatgt ccaaccttcg gcctctcctg cctgcacgag cttgaatgcg
     2641 atttccaaga gatcatcgcc gagggcgatg gtgtcgaggt tcttatccag cctatatcga
     2701 agcatttgcc gcagccggaa tactgttggg taggcatcta ccaatgcaga cacgaggcgt
     2761 tcgtactgtc gtccattcag cctcattgct tctccatcaa cacaattgtt gtcaattggg
     2821 tgccgttgac ttcaaatgcg gcgcaccatc gctttggtag tgctgcataa tctgtctccc
     2881 tcactgcggg ttcgctccgt ctaccccaaa tgtaggtggt agagaagcag atcaagcagt
     2941 gctgacgcta gcggtgggaa gatgcctgtg gccttggaat gctgctggcg tggtgacatg
     3001 acacaaagcc gtgccggtga cagtgctgac tatcatgcta gtttaccacc aacaatggtg
     3061 atctggtagc tagtgggcga ccttcagctg ttgcagcagt aggcttaccc gctcgtacgc
     3121 atcccgctcc cgaagggctc gctcctccgg atcgtccagc ccgtcgaagt cggccacaaa
     3181 ccgtggccct acatgctccg gcgaggcgaa cttctcagcg atcttactaa gtccttctcg
     3241 cacgagacca tggtccaagt gcggctggaa ctctttcacg agagcatcga gtccacctgg
     3301 atagtaacga aggcagaagt agatgtccca ggcgtccttc tccttcatcc ggtcgtggag
     3361 tgccattccc ttcatcacga gaaacggcac aatggctgca actctgacgt cagcggtgtc
     3421 cttccctccg ccgggcagcg tcccgctgat ctcgacgttc ctgaccatct caaaggctag
     3481 atcggcgcca cgagccttgc gagcccgcac gtctaggact ttctgtgtgc gatgcccgct
     3541 ccccgttccc tggtactccc ctgccaacag gtcgacctcg accgagactt cgtggtcccc
     3601 aactcgcacc agtctgtgga agacaaacgg ttgattcccc tgtcggtagc ccctgaacag
     3661 cagcaactcc tggatcgttc ggtatccggc ctcttggagg ttacggtggt tcaatgccaa
     3721 gtccacatcc atgctgccca cgtggtgaat gtctcctcgg gagatcagca gctcgggaac
     3781 ccagccgcct atcaccacga tgtcgtcacg gtactcacca agcaggtggc agagttccag
     3841 cagcacggag cgcgaggcct ctaccgcttg ggcagtgtag tctactcggc ttgttgccat
     3901 cggggtttga tgacctcctc cagcagggca ttggccgctt cctcgctgcg acctttcatg
     3961 gtgcgaagat ccaagtacag ttggatagga gacgcgactc tagtgccgcg gacatcctct
     4021 gctccataga acacgccctc gtcgtagggg acgacgagat tcacattgct tccgcttggc
     4081 acctgcttga ggcctagcct tgaggctatg cggtccaccg agtcgggcac gtaggcggtg
     4141 acacgggagt agcggacaaa tggtgcaaac ctcgctgctc ctgagaagcc cgtcagcacg
     4201 gaacgagtct catcacttgt ccctacccct gccaactcgt actcgacttc ggagatttcc
     4261 ttggcagtgt agaagtcgac agtctggttt cgacggagac cgtagttgcc agtccactca
     4321 gccagcactc tctcaggctt gcttagacgg aacccgtcca cgcccacctg gatccattcc
     4381 ctgtcggcca ggagccgctt tacgttggca acctgcccaa gactgacccg agcttcgttc
     4441 gcgagtgcct cggttttcca ggccctgttc ggtctctgca gcaggacccg aagcacccgt
     4501 tctgccttag gggagtagag agtgcgaaga tcgcgtcgtt ggacaaaagg attgggtctg
     4561 cactctctgc gaatgaagac ctggccaaat gcgaggtagc agtttccggc caagtcgagg
     4621 tagcccaccc cttcttggcg gcaaatctct gccgactcag gtgatatgta tggagcaacg
     4681 aagatcccgt aggcgttggg cttgagttgt atgtatcgca gcagttggtt gatggcttcc
     4741 cgggcaaggc gaggctggcc cgtgcctttc acctcaacga cgagcagttg tttgcctttc
     4801 ggcaagtcga cctccatgat gagatcgggc cttggccggt tattccaacc ttcgaggcga
     4861 cgaacatcac gaaggaatgg gacctcgttc aggcagttcc tgagagcctg ctcggtagca
     4921 gcttccacct ctgaatgcgt tttcactgtt ggtggccttt tcaacaaatg ctgaaaagca
     4981 gcatatcagt gaaaacgacc agtgtcaaca tcttggagcc cgaaatcggg tggttgcccg
     5041 agtgagaggc cgtccacttg atgccctatc agtaccttcg ggacgctgag gccggtggtt
     5101 caaatccact cgcccagacc gcctctagcc ctccggcatt tcgccggagg gcttctctct
     5161 tactagcatc tctctcgtcc tcagttctgc ctgagagacc gcgtggtggg ccacgcctcc
     5221 ctccatcttc gcgccttcag agtagttgag gttgctgcct gcaggggcta gaatccactc
     5281 cggccgaagg ggcggtgcga tcgctccccc gtggcgacgc tgccaggcaa tgctcgccgg
     5341 aaggagatct gaatgactcc gatcgttgtc gaagtcgggt gtaagaccgc gggcgttgcc
     5401 gtcggcgtgc atgacatcgg accggtcgcc ctccaattga tggaggaacg gctgaccgtg
     5461 gctgagttaa tccgacgcac ggtcgaagag caagtcaggg agttgctggt caggcgcaag
     5521 cttgcggctg accaggcgcg acagctgctc gaccgtcaat atctgacgga gaaagaaata
     5581 gcccgccagg cagagcacgg cgcagtccgc tatccatcgg gtcgtgtccg gcaggaaccc
     5641 cccattgacc cggctgccga ggtgtggaag gcactgcgcg gcttcgagaa tggctcctat
     5701 ctcattctga tagatggccg acggatggaa cgtctagaag agacgatcga cttcgcaccg
     5761 ggcgttcagg tgaccttcct cagggtgatg ccactgatgg gaggatagcc ctgcacagat
     5821 agccgctggg gggaagagag atgtttcgtc tcatgcttcg aggcgacagg gacggtcgcc
     5881 tggaagagca gatccgcttg tgtatcgagc gctactggga cttccaggag ttggaagcat
     5941 ggtgcaagac gaaccgctgg gatcttccgg tggctgccct ggacacagcg tacccgccgt
     6001 tgacgcacag tctcgatcat ctcagagcgc gttggtccga ggacaagctg agcaggctcg
     6061 ccggcgacat gtacttcaat ccagcccgat ggcagcattc ctacatgacc gattggaccc
     6121 agcgatgggt gatgcagttg gtccttgtgg caatactgct gcgattcccc cctcctgacc
     6181 tgttcactcg gattgagcag cgcatgaagc tgatcttcga gcaccccctc gacggcgagg
     6241 agataccagc gccatttcga cagagaagtt ggcccccgga tctgtatctt agtcccgccc
     6301 tggaggattg ccttaagctg ctgcgagcgt tgcccgcaac agcggagaag atcgccctcg
     6361 tcgagaaggt ggcactcggg gaactggacc gaggaccgtt cgccgtgcct gcttactcca
     6421 gttccgatcg agactccatg agaagagcct acgacatcta tgtagggtcc ttggtgtacc
     6481 gtgcctcctg gatgtgggag ttcctgtacg atcgcgggcg cttcggctat gaatcattct
     6541 gccgagcgac cgagcacctt ccgactttac tgagcacgtt gagcatggag catactctct
     6601 acggtggtct tactcccttt tacgccggag tgagcgaggc tttcagtgcg acgctgaagg
     6661 ggtactgcga taggcttgcc tgggaactgg ctcaggacat cagcgaggcc acctgcaaac
     6721 ggctgattga gatccatgag ttgcgaggaa gccgttacct tgtggaggct ggccgccaac
     6781 acagcaggca cggactaggc aggctcatct acaaggcgtt cgagtatcca gagaccgtgc
     6841 agtccgcgat agtgcacatc gcccgggcag ctgacaccga accatccttg tctgaggatc
     6901 gtcagagggt tgtgaaggag ttgcgggcac tccctatcga cacgctcaaa tacctgctgc
     6961 ctatcgcccc cggcagccga catctgctct gtgaggccct cggctggcaa gccgcagcac
     7021 cattgctcga gcagttcgtg ctgatcgggc ggccggacag gcagggcggc ttttcgccgg
     7081 tcgggtcggg gaacccggag gatccatcgg tcggcgtgct ggatgtgccg acggttcgtg
     7141 aggcccttgg cgtggcagga gagcctttga ccagggaggt gctctcgctc ttccgcaaag
     7201 cgaaggtggg cgccgaagac gtggtgatgc tgatcgaggc cgtcgctggc tgggaccggc
     7261 cacaggtgga gaagagcttc accaagcgca agcaggccgc agtcaaggcc tacgggctgc
     7321 tgcccctgga gcgaggcgag gacgaggcgc tggatcgcta tctgtctctc caagcgttcg
     7381 cccgtgaaag caagaagctc ggctcggagc gtcaggccaa cgagaccgct gctgttcagg
     7441 cggcgctggc taacctggct caggttgccg gctttccaga tgcggtccgc ttggagtggg
     7501 cgatggaggg gcggctgagt acgacggtgg cggcacccga ccggacctgg atggtggatg
     7561 actaccgaat cgagctgaga ctggacgtag acagacctga actcgctttc tggcgcggag
     7621 atcggccact caagtcacct tcggaggctg tgcggaggag tgaggtctac gcggaagcaa
     7681 aagaggctgt tgcccagttg cgggcgcaga ttgcgaggtt tcgccatgcg ctggagaaca
     7741 tgcttgcagt cggggatgcc ctctcacacg atgacctgga gatgctgcta cgtttgcccg
     7801 tgctcggcgc gatgcttcca cggcttgtcc ttcgcggtga cgacggcatc tttggtctct
     7861 tcgatcccaa ggaccatgtt ctgatacccc tgaaaggggg accgagatcg ttagcccatc
     7921 cagcccggat tgctcacccc tacgacctcc tcttggcggg acagacgtcg gcgtggcaga
     7981 gggagatcgt ccgccggcgc atagtgcagc ccttcaagca gctgttccgc gaggtgtacc
     8041 tgcttactcc ggcagaagcg gagacagtga actactcgaa ccgattcgcc ggacacgtag
     8101 tcgacggcag ggtggcatcc aggctgcttc aggcacgggg ttggcagatc gaaaccggtg
     8161 atattgcgtt gccctacaag ctgttccgca agtgcggcct cgcgtctttc ttcgtcttcc
     8221 ccgacgcagg acactacctg ggtgaactgg acacagtgac aagcgatctg gtctatttcc
     8281 agcctatcga cgcccgcgat cggtggcagg gctggcgtga tgagacccgc gtcccgctgg
     8341 cagacgtgcc acccatcgtc ttctcggagg tcatgcggga tgcggatctg gtggtttccg
     8401 ttgctcaacg cggcgaggac ggactgctct ccgaagaagc atatcagcag cgggcggcgg
     8461 tggttacatc cttgctggag gacctgagac tgcccggggt gactgtcgag ggccactttg
     8521 cccatgtcga ggggaagctc gcccgctatc gcgtccacct gggcagcgca gttgtccaca
     8581 tcgaaccggg caactacctc tgcgttgtcc ccgccaagcg aggcaatcat ccggagagac
     8641 tcttcctgcc ctttgccgac gatggcgaca gcaagatcac cgaggtcatc agcaagatcc
     8701 tcctgctggt aagcgacgac aggatcaagg atgacagcat tctgcggcag attcgtgtag
     8761 cgcgatcgcc acaggacggg tgagttgcag ccgcatcgcc agttccatgc cgcaagaccg
     8821 ttgcgagtgc agccaaattc cggtcgcccg ctttgatgcc ctactagtgc actcaggacg
     8881 ctgaggccgg tagttcgaag ccactcgccc cgaccacaaa gcccgccgga aatctccggc
     8941 gggcttttcg tgcccccact cttcctgcaa ccgcgatgtc acacgggatt ccttgcgttc
     9001 cacgcggctg gcgacttccc cttggcagca cgctaggttg tggtgcctgc tctcaggatc
     9061 cagcttgttg ccgttgcgca gtcggatgcg gtggagtgct atgatagcct ttgccagctg
     9121 tgccgcatgg gccctcacga gaattcggga gacttgtcag tgcggaagag agattgaaga
     9181 ctcacctaag agccgtctcg cggccgtcct gtctgctgct cgcccggtta tcctcgccct
     9241 gctcgcctcc ctcgctgctg ccgcgccgca gcctccctac ttccagccgt tcgacgtgga
     9301 gactgccctc cagccgaacg gcgacttcgt cgtcatcgag agccagacgg ctgtcttcgg
     9361 cttgcagtcg gcgaggcacg gcttccgtgt tattccgatg gatcgcgctg agcaggggga
     9421 ttacttcaag aagcgaggct ggatacgcgc attcgcgact acgctcagca caagtgcaac
     9481 gagttcgcac gccgagggga cgagcaccct cggcacagcc gaaggcccag gctccgagcc
     9541 gactctggac cctccaagtc cctaggttca acaggcgctg aggtgccggg tgcggactgc
     9601 tggttgcagc caactgccag cagcattagg gatcaccccg cctcggcggc gcgttcggcc
     9661 ctcctcgcga tataataaat gaacctacct ggttgaaggc gttgcatacg ccctgagggt
     9721 cctcaggtat ttgcaccctg gctgggcggc gcgaggagga ggatctcaat gaagagtaga
     9781 agatcgtggg caatgttgct cgcccccgct ttcttggtgg ttctgctgct ggcgttgagc
     9841 tcgtcgagct tgctacaggc tgatgggaag taccccgcag cggcttctgc cgcttacgca
     9901 gccgtgcttg cggaggaaag tagcccaacg gcaacaccta ccagtgcggc gacttcgaca
     9961 aagaccgcaa cggctactct tagcagtacg tctacgccta cgcagatagc ggcacccacc
    10021 tccccctccg catctccaac cagcacatcg acaacgtccc cgaccggctc gccaaccacc
    10081 ccaaccgcga ccgctctggt aaccagctcg ccgaccccga cgaagacggc aacgctggcc
    10141 ccgactgcga cgcctacgcc gccacccctc cgcaggggag gctcatccct ttcgagcgcc
    10201 cccgcccctt cgaagctcag tgtgcagagc ggcccggtat tcgggcctct gggtggcagc
    10261 ccctcgggca ccaacctcgc ggcggcggct aacggcggaa gcatcgccaa caagtcctcc
    10321 gaatacctga ccctacttgc cacgaacctg atcgacggaa acacgaacac gacctggggc
    10381 acgttctcgc ccgcttccgg gttctggatc atcgtcgacc tggggagtat ctacaccatc
    10441 ggcagcattc gggtgaatac ctcctccgcc ggagatcctg acggggcagg aagctgctat
    10501 cccagatcga tcaacgttct aacttcgacc acgagcacgg accccgtctc cgggttcaca
    10561 ctcgcagggt ctttcgaggc gacgcagaac gactggacgg actattcctt ctcgcccgta
    10621 gctgctcgct acgtgttgct gagtatccag accatctggc ccggtggggg tgagaacgcc
    10681 gccgatgtcg ccgagctcga ggtcttcacg cccaacgcgg caccgaccat ttccggcacg
    10741 gccccgcact ccttcggaga cgagcaccta gtgaaccccg cccaggtcag cgcgtggatg
    10801 gccgggctta ctaccggtga cacggatggg aataccgttg gaatcgcgat cacaggaacg
    10861 accggcaacg gtacctggag gtactcgatc gattcacagg ccacctacac tgccctcccc
    10921 gcagtaagcg acacgagtgc cctgttgctg cgaccttcgg actggatctc ctacaccccg
    10981 gacttacaga acggggagac aataacgctc accgtaaggg gctgggacca gacaaccggg
    11041 tccgccggaa gttcagccga catctcgatc aacggcggct ggactgcctt cagcacgggt
    11101 accgcgacga tttcttcaac gattgccgct atcaacgatg ctccaactct ctcgggaggc
    11161 cccttcatcc ttacagggac caacgaagac actgcgagct caggaaccct gatatcgacg
    11221 atccttgcag ggctgaccta cgccgacgta gactcggggg cacaatcggg tatcgcagtg
    11281 ttcggcaaga ccggcaacgg cacctggcag tattcgacag acggcacctc tggctggacc
    11341 tctatcggca gcccaaccgt aaactccggg gtgctgctgg cctcctcgac atacatccgc
    11401 tacgtaccta acggccacaa tggcgagaca gcgaccctct cgttctccgg ttgggacaga
    11461 accactggcg cacccagcgc gaacggctct cccgctactg cggaccccac ccccggtggt
    11521 ggtacgactg ccttcagcac cggcaccgcg caggcttcga tcacggtagc gtccgtcaac
    11581 gatgccccga tcatcggcag cggcacctcc accctgacag ccacgatcgc caacgtcgcc
    11641 agcaccccca agatggtgaa tcagatgttt tccgacttcg gctataccga cgtggacttg
    11701 gcggcgcaac cggggatcgc ggtcacggcc aagactggtc tgggcacctg ggagtattcc
    11761 gtcgacgccg tcacctggac gagcttcgga gcggtgagcg acagtgccgc attgctgctc
    11821 gatagtgaca gcttcgtccg ctatacgggg gatggaagca gcggggagac ggcgaccttc
    11881 accttccgag catgggaccg gacctcgggc acggccagca cttacggcac gccgtgcacc
    11941 gccgacacaa ccaccaacgg cggtaccagc gcctttagca ccggcaccgc aatggcccag
    12001 atcagcgtga gcgccctgaa ccacgcccca acgatgtcag ggggggggca tcactctcgc
    12061 gagcacgaac gaggataccg ctggcttcag ccagggggtg ttcgcgttcc tgatgaactt
    12121 cggagcgagc gacagcgacg gcgacacgat ggggatcgcc gtcatcggca cggcgggcaa
    12181 cggcacctgg
//
LOCUS       DATALS010000558         3760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_83207,
            whole genome shotgun sequence.
ACCESSION   DATALS010000558 DATALS010000000
VERSION     DATALS010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83207"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1753
                     /locus_tag="VHS06_12440"
     CDS             137..1753
                     /locus_tag="VHS06_12440"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2988961.1"
                     /translation="MSSQDRLRALAGSVWAGYCAALGMVAIVSLFIGLIRGALHVGNL
                     SMLYLVAVLAAAIAFGRGPAVLVSVTAFLTFNWFFVEPLHSFWVSDPGEWIALLLLLM
                     TAVLTGQLAAAQRLRAEEAEQRKRDAIVLYDVVRLISATNPESALKDVAERLRRELDL
                     SAVVIELSPEDEHCTQAASGNAEAIRRATGPRSIPAHLLEQGSTPTALQRGQPGRWVR
                     IVPPHSLPSGRGIDDNRVHLVPVKAGDKRVGDLILVTAAKGREFSNTEDRLLSTVAAQ
                     IGLAVERLQLRKEATDAEVLRRADALKTALLNAVSHDLRTPLASIIAAAGSLLQNAVA
                     WTDQEREEFAETSESEAQRLNRIVGNLLDLSRIKAGTLQPEKGWYDIESLVEEVLGRL
                     SGVTAGHTVRIDIPEDLPPVLLDYVEIDEVLSNLVENSAKFAPPETEIRIGARNQGGE
                     IRIEVENSGPGIQQDTLHLLFEPFYRGGKGDTHPQGTGLGLAVAKGLVEAHGGRIWAE
                     NRAEGGARFVFTLPLLEEIPPREGPVEVVQ"
     gene            1750..2451
                     /locus_tag="VHS06_12445"
     CDS             1750..2451
                     /locus_tag="VHS06_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2988962.1"
                     /translation="MSKAGPMILVVDDEQAILRSLRMNLTRHGFDVEVAETAREALDI
                     YARRHPDLILLDLGLPDMDGMEVIREVRARDATPIVILSVRGAEADKIAALDLGADDY
                     LTKPFGVGELLARLRVALRHALRSPTTNSTLFRTGELEVDLGQRMVTVRGNEVHLSPT
                     EYQLLKAFISNPNKVLTDRMLLQQVWGPEYGSEGHYLHVYVGRLRKKLETDPQHPNYI
                     VTEPGVGYRLLAEGN"
     gene            2585..2863
                     /locus_tag="VHS06_12450"
     CDS             2585..2863
                     /locus_tag="VHS06_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988963.1"
                     /translation="MGNQSVAQEPFSPEYMEMLGGWTDALYAACDAANRANRPITVTG
                     RLQTIVEGAPILRSLCRELAEGSGLKAELTMDGSRFTVRFCKAVSAAM"
     gene            3103..>3760
                     /locus_tag="VHS06_12455"
     CDS             3103..>3760
                     /locus_tag="VHS06_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX2988964.1"
                     /translation="MSNEQRVPSRTPEDDIHPPVGFHPDLVWKQEVVGSRPGDRVVRI
                     ARHRHFQGATAGVLIPRREAVEPKGRAGKLLMNAKHFLVGQPIATAMEGQERLSKFKA
                     LAVLSSDALSSVAYGTEAAMRALILAGVGALALTMPISIVIAMLLIIVATSYQQTIQG
                     YPGGGGSYIVAHENLGPIPGLTAAAALLVDYVLTVAVSVAAGVAALVSGFPPLLPYVV
                     P"
BASE COUNT          766 a         1155 c         1165 g          674 t
ORIGIN      
        1 cgcagcacct ccgcgtccgt tgcctccttg cgcagctgta acctctccac tgccaggcct
       61 atctgggctg caacagtgga taggagcctg tcctctgtga tagaatctca ccgaaccacg
      121 cggttgtgga gacgggatga gcagtcaaga tcggttgcgc gcactggcag gtagtgtttg
      181 ggccggctat tgcgccgccc tcggcatggt agcgatagta agcctcttca ttgggttgat
      241 ccgaggtgcc ctgcacgtcg ggaacctatc catgctctac ctcgtcgccg ttctggcagc
      301 agccatcgcc ttcggcagag ggccggcggt cctcgtctcg gtaaccgcct tcctcacctt
      361 caactggttc ttcgtggagc cgctccacag cttctgggta tccgaccccg gggagtggat
      421 tgccctcctc ctcctcctga tgaccgccgt attgaccgga caactggctg cggcccagag
      481 gctacgtgcc gaggaagcgg agcagcgaaa acgggatgcc atcgtgttat acgacgtggt
      541 tcgcctgata agcgccacaa atccggagtc tgcactgaaa gatgtggccg agcggctgcg
      601 ccgtgagctg gacctgtccg cggttgtgat tgaactgtcg cccgaagacg agcattgcac
      661 acaggctgca agcggcaacg cggaagccat acgccgggcg accggcccca ggtcgatacc
      721 cgctcacctg ctagagcagg gctcaacacc aacggcgctg cagcgtggcc aaccgggcag
      781 gtgggtgcgc atcgttccgc ctcactcgct tccctccggc cgaggtatcg acgacaatcg
      841 cgttcatctc gtccccgtga aggccggcga caagagggtg ggagatctca tcctggtaac
      901 ggcagccaaa gggagggaat tcagcaacac agaggaccga cttctctcca ctgttgcagc
      961 ccagataggc ctggcagtgg agaggttaca gctgcgcaag gaggcaacgg acgcggaggt
     1021 gctgcggcgc gccgacgccc tcaagactgc cctgctgaac gccgtctccc acgacctgcg
     1081 caccccgctc gcctccatca tcgccgcggc gggcagcctg ctgcagaacg ccgtggcctg
     1141 gaccgaccag gagagagaag agttcgcgga aaccagcgag agtgaggccc agcggctgaa
     1201 ccggatagtc ggcaatctgc tggatctatc gcggatcaag gcagggaccc tccaacctga
     1261 gaagggctgg tacgacatcg agagcctggt agaggaggtg ctgggcaggt tgagtggggt
     1321 gaccgccggc cataccgtac ggatcgatat cccggaggac ctgccccccg tcctgctgga
     1381 ctacgtggag atcgacgaag tcctctccaa cctggtggag aactctgcca agttcgcgcc
     1441 tccagaaacg gagatccgga taggcgcaag gaaccagggt ggggagatac gaattgaggt
     1501 agagaactcc ggaccaggca tacagcaaga taccctccat ctcctgttcg agccgttcta
     1561 tcgcggtgga aagggagaca ctcaccctca aggcaccggc ctggggctgg cagtcgccaa
     1621 ggggctggtg gaggcccacg gtggccggat ctgggcggaa aaccgagcgg agggcggggc
     1681 gcgctttgtc tttaccttgc cgctgctgga ggagataccg cccagagaag ggccggtaga
     1741 ggttgtacaa tgagcaaagc agggccgatg atcctcgtgg tggacgacga gcaggcaatc
     1801 ctcaggtcgt tgaggatgaa cctcacccgc catggcttcg acgtggaggt cgccgaaacc
     1861 gcccgggagg cgttggatat ctatgcccga cgccacccgg atctgatcct gttggacctc
     1921 ggtctaccgg acatggacgg gatggaggta attcgagagg ttcgagccag ggatgcgact
     1981 cccatcgtga tcctctcggt gcgaggtgcg gaagccgaca agatcgcggc cctggatctg
     2041 ggtgcagacg attatctcac caaacccttc ggagtagggg agttgctggc ccgactgaga
     2101 gtggcgctcc gccacgccct acgctcacct accacaaact caacactgtt ccggacaggg
     2161 gaactggagg tggacctcgg ccagcgcatg gtgactgttc gagggaacga ggttcatctc
     2221 tccccgaccg agtaccagct cctaaaagcg ttcatcagca atcccaacaa ggtcctcaca
     2281 gatcggatgc tgctgcagca ggtgtggggg ccagagtacg ggtccgaggg acactacctc
     2341 cacgtctacg tcggccgcct gaggaagaag ctcgagacgg atccacagca ccccaactac
     2401 atagtcacag agcccggggt cggataccgg ctgctggccg aagggaactg acctcctctc
     2461 cctcacccca tcccttatct cctatctccc aacaatacgt tgagcaaacg tttatggacc
     2521 acgcccatcc gttgacacca cattgagcgg atatggagca gaatatccct cgaggtgaat
     2581 gacgatggga aaccagtcgg tagcgcaaga accgtttagc cctgagtaca tggaaatgct
     2641 gggtggatgg accgacgcgc tgtacgcggc gtgcgatgcc gccaaccgcg cgaacagacc
     2701 tatcaccgtg acgggcagac tccagacaat tgtagaagga gcacccatcc tgcgctccct
     2761 gtgtcgtgaa cttgccgagg gctccggcct gaaggctgag ttgaccatgg atggaagccg
     2821 ctttaccgtt cgattctgca aggcagtcag cgccgcgatg tgatactcac agaaatcctc
     2881 ttcggccgcc ggggttcccc caccctgagg ctacctggac cgaccctcct tgcaccgtcc
     2941 tctccgtcgc acttctgccg gtctgctcgc tcatgagggg tgtttggtca ccataggaca
     3001 atccggccat atggctcaga gcatcactgt gtataatcac tactattacg gcagaacact
     3061 gcatggggtg gcagtcaaca cccaaacaga ggaaacaacg agatgagtaa cgaacaacgc
     3121 gtcccttccc gaacgccgga agatgacatc cacccacccg taggtttcca cccagacctg
     3181 gtgtggaaac aagaggtggt gggctccagg ccgggggatc gggtggtgag gatagcacgg
     3241 caccgccatt tccagggcgc gaccgcgggc gtgttgatcc cgcgacggga ggcagtggag
     3301 ccaaagggaa gggcgggcaa gctgttgatg aatgccaagc acttcctggt ggggcaaccc
     3361 atcgccacgg ccatggaggg ccaggagcgg ctgagcaagt tcaaggcgct tgcggtcctc
     3421 tcctccgacg ccctctcctc ggtggcgtac ggcaccgagg ccgccatgcg agccctgatc
     3481 ctggccggag tgggggccct ggcgctgacg atgccgatct ctatcgtcat cgccatgttg
     3541 cttatcatcg ttgccaccag ctaccagcag accatccagg gctatcccgg cggggggggg
     3601 tcatacatcg ttgcgcacga aaacctgggt ccaatacccg gcctcactgc cgcggcggca
     3661 ctgttggtgg actacgtcct taccgtggcc gtatctgtgg ctgccggcgt cgccgcactc
     3721 gtttccgggt ttccgccgct gttaccctac gtggtaccca
//
LOCUS       DATALS010000559        11462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_83656,
            whole genome shotgun sequence.
ACCESSION   DATALS010000559 DATALS010000000
VERSION     DATALS010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11462
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83656"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1019..1906
                     /locus_tag="VHS06_12460"
     CDS             1019..1906
                     /locus_tag="VHS06_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX2988965.1"
                     /translation="MAPSLDGVSPEFIAIDVETANASLSSICQIGLVAFENNAPAVRW
                     KTLINPEDYFDEVNVSIHGITKRMVRGAPTFPAIVEDVHKWLSGRVAVCHTAFDRTAI
                     GRVAEKYGLSPVECTWLDSARVARRTWPDCSQRGYGLLDLASRLDINFCPHDAQEDAE
                     AAGMILVRAMAETGMGMAEWMERVKKPIDPTRPSSGPIHLDGNPEGEFYGEVLVFTGA
                     LSLPRVEAAKLAAQAGCHVDDGVTKHTTLLVVGDQDLTKLAGHEKSSKHRKAESLIAK
                     GQPIRILAESDFRLLVGMS"
     gene            2320..3009
                     /locus_tag="VHS06_12465"
     CDS             2320..3009
                     /locus_tag="VHS06_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988966.1"
                     /translation="MQAPILSAPNRKYAGLSKNTQYPPLKLRWWAFAALPGLSMTIAY
                     HLFPQLAVAPFGTALWVTGAALLLPFVMSVLQYLAEIARITIFRLRAYPSLEGDARQL
                     SADLQAERSRLSSLMGLIPEEAPRHEFRAVRRDPITGEAYFILKKNEAVSLNEGDSLL
                     AVDRYDSQLMCSLRVVSIEMDSYRVQVTDVRDALWWGYMQVQADLHLHAPLETDTVAV
                     FVPLNKENSHE"
     gene            3002..3421
                     /locus_tag="VHS06_12470"
     CDS             3002..3421
                     /locus_tag="VHS06_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988967.1"
                     /translation="MNEKPLEGKVAQILNERELVINIGSAAGVIRGMRFAVLAATPVD
                     VKDPDTGDVIDVVDRAKTHVEATEVRERITICGTYHRRYVGGSIQQVLKEMEQFSGWQ
                     RQTFRIGDGGLPKPIDPTESYVKIGDRVKLVIEPNEE"
     gene            3747..4028
                     /locus_tag="VHS06_12475"
     CDS             3747..4028
                     /locus_tag="VHS06_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988968.1"
                     /translation="MATVLARWVEVVVAPQLDAWTYEDYEPLLRLHVLPTLCDMPVGR
                     LTGQHLETLYAKKRTEPQDKRTEPLFERRIQQSTQCPIPRSSTSTTSGS"
     gene            4150..5004
                     /locus_tag="VHS06_12480"
                     /pseudo
     CDS             4150..5004
                     /locus_tag="VHS06_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            5390..6520
                     /locus_tag="VHS06_12485"
     CDS             5390..6520
                     /locus_tag="VHS06_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX2988969.1"
                     /translation="MSRQQERTYGVGSVYQRKSDGRWVAKLGNDGKPITRYAATEKEA
                     KTILVALQRECGQNGTLATPGNRTVNDLLDLWLQTAQPNLKPRTFHDYQARCKRHLRP
                     VLGHVKLSKLSPERVQAFYTKLLSCGIEREALYSHAMLHRACKMAVLWGWLAQNPCDR
                     VIRPKHQAERKEMWSIEELHRFLEGTRDQWLHPLWVVAASTGCRLSELQGLRWQDVDL
                     AASTLRVSRSLHRIEGQWVESTPKTRAGNRTMVLPAAAVAALKGQRAQQAEWRLKAGP
                     QWQDTALVFTGEHGQPLAQSTVQKAMHREIERLDLPPLTPHGLRHMAASLLLSKGLPV
                     PAVSARLGHASPNITMSIYAHAMKQQDQQAAALMQEVMNSRA"
     gene            complement(6532..7536)
                     /locus_tag="VHS06_12490"
     CDS             complement(6532..7536)
                     /locus_tag="VHS06_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988970.1"
                     /translation="MAEEEPVGHREIIEEFFGRELSEEEREGYRPEDEEVPDDIARTL
                     PPRLEEHARQWQLDWEWPAQAQIGRISQSVVEDPQFQYFLKIMVDPWRRSWPALPDLM
                     SAHAWLRDNSIDLEAEARRALGEVERGEAVQIEPSLKVVPTGPDFEMVYLPPVETAKD
                     DQVERLYLDLSNPVLPGEVEYKVTQHCLKQLSRAAKVVSKRSGCWSLGQATLFLLTNQ
                     VPQVPRVRLWTSRLPGGGVRSLIEVYGPLNYRETRALHRTMRERTGAYRKRGSTPADL
                     ELVRFVEASMAPRPSSRAKRSWKSLLSTWNDMHPERAFTRPNALQTAYSRAKARTASI
                     "
     gene            7863..8273
                     /locus_tag="VHS06_12495"
     CDS             7863..8273
                     /locus_tag="VHS06_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988971.1"
                     /translation="MTTLGLRQEDFSPFALAIRKGGQWRTIGKLVLAKPLEEIVLAFP
                     YRNRVSLDHVSAPVSVLKHAHHEGAKWWVVRLDREGICLGLPMVMIPKVGFRLDEGDS
                     CEIAVELADFEPIDWQDWPYVERLVHFPLEATHA"
     gene            8266..8754
                     /locus_tag="VHS06_12500"
     CDS             8266..8754
                     /locus_tag="VHS06_12500"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEX2988972.1"
                     /translation="MPDATRLKAIQGYLKRGWYVIPLCWPSAGECGCGRGHTGKEIGK
                     APLLGSGYQNRRSTESDVNEWWTRWPDANIGILLEPSGLLVIDCDGVEALREARLFGL
                     PEGPVVRSGNGVHIYYKAGDATGRATKQGNSHAIDVLASGFVVAPPSRHRNGHTYEWR
                     RS"
     gene            8778..10523
                     /locus_tag="VHS06_12505"
     CDS             8778..10523
                     /locus_tag="VHS06_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HEX2988973.1"
                     /translation="MWAVQVLTKTPSAGPSPRIAVDSLPPVRLGDKGVRLWQGELAVD
                     KADGRLKASDVVSEIDTSETLCALGGELAKAGAAETTIRAALAERDRVLGYHKYDARK
                     GGGLEEYARIAAKVTTEKPMVVDLHTTDMGNAQRLVALHGADLRYCQANRQWYLWDGR
                     RWAPDGTGEVYRRAQDTVAVMYHQALKEPDHDRKEALLKWALRSEGRDRIEAMVALAR
                     SSQEMAVTPAQLDANPWLLNVMNGTLDLRTGHTRPPNREDLITKLAPVQYNRDANLPL
                     WDRFITDLTNDDPELAAFLQRAVGYSLTGDTREEVLFFGHGPSATGKSTFIEAVKATF
                     GDYASTADFETFLARRDAGGPRNDIARLGGSRFVASVEVDEGKRLAEGLIKMLTGGDT
                     VSARFLYQETFEFRPRFKLWLMANDPPKVRDNDDALWRRILRVPFVHSVPKDRRDPAV
                     KATLRDPKTAGPAVLAWAVRGCLEWQKGGLKVPQVVEHSTETYRQEMDPLRLFLDERC
                     TLDQGRWTPSAELRSAYETWARESGEPDILSGRAFAERLRALGCTSTKKRVQTTTKDR
                     SVSTWGWQGIGLVEV"
     gene            10681..10998
                     /locus_tag="VHS06_12510"
     CDS             10681..10998
                     /locus_tag="VHS06_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988974.1"
                     /translation="MAASRKMHADPAMLKLLEKQVLQIISKPRNWWSPRGGFWLDEIG
                     WGTLRAYGLKRSEAEAAIDSLWNKGMITISPEEGNRVVRVAKPARARDDASIEELLDD
                     FIP"
     gene            11150..>11462
                     /locus_tag="VHS06_12515"
     CDS             11150..>11462
                     /locus_tag="VHS06_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988975.1"
                     /translation="MQDVDTQVVTALRQANFTAEEVQSIYQSFPIGTPKRDSAYLARL
                     VEDADRKIARTEAEARRVRGLNFEVVKVVHVNGLYPAYLLTVTLQDESEHTVSVAHAA
                     IT"
BASE COUNT         2584 a         3256 c         3347 g         2275 t
ORIGIN      
        1 cgccatgctg gcaatctact cgtgccccgt gtcaccgagc gtgtcatcgg cagcaggaga
       61 gtgcgacagc ccgtaccatt cttctcttac cttttcggca ccagaaaggg aatctttagg
      121 ggggctcggt taggtctgtg ggatcgcagc cgatgcagca actaccggag actcggtttc
      181 tcgttgctga cggcttggga cctcaacttc ttgatgggag cggtaaggga agtgcagcct
      241 ggatttcgct ccaagatgca acactcgaga ggaacagcgc aaaagaaaaa ggcccccagc
      301 ctttgctgag agcccccttc tgccggtacc ggggagggga aacgatccaa agccctattc
      361 gcggcgccgc ttccttcaag gttacgcact cgagaggcta ggatctaccg tgccagagcc
      421 agtctcaatt gacaggtatt cgtggagacc ccccgcatgt ggtgccaaac aaggccgcaa
      481 agctctctcg ctttcctcca actcctccaa gttcccgcgt ctccagctac gcgcatagac
      541 acaaacctta acctgagccc tggatatggc atctacaaga tgctcacgcc tgcgaggtgc
      601 tctagccagg acatagcggt gagtccgact aacgtttggg agaccagcca taggccttgt
      661 agaataacct ctctcgtgat aggctactat tgagctacac tgttgtagct cctgctgaaa
      721 ccgccacttc tcactatcgg cactaccacg cccgcagctc actgtgagat tcgcagcaag
      781 ggtcgaactc tgctcacctt caaagcgttg tggcaacgac acgctcggcg cgtttacatg
      841 ttcttgtcct ccactctggt ccccattggg ggattgccgc atcactgaac cgttcgccac
      901 acgcactagg caactgcgca ttggccgatg atcgcatctg gataagcgaa agggcattga
      961 gatggaaatc aggattggca tctgggacac aatccgcccg gatcccaagg gatcaccatt
     1021 ggcacctagt ctggacgggg tgtctccaga gttcattgcg atcgacgtcg aaacggccaa
     1081 tgccagtctc tccagtatct gccagatcgg cttggtggcc ttcgagaaca atgctccggc
     1141 tgtacgatgg aagaccctca tcaatccgga agactacttc gacgaagtca acgtctccat
     1201 ccacggcatt accaagcgga tggtccgagg tgcccctacc ttcccggcga ttgttgagga
     1261 tgttcataag tggctttcag ggagggttgc ggtctgccac acagccttcg atcggacagc
     1321 gataggccgg gtagcagaga agtatgggtt gtctcccgtt gaatgcacat ggcttgatag
     1381 cgcgagagtg gctcgccgaa cctggcccga ttgttcgcag cgaggttacg ggctcctgga
     1441 cctagcctcc aggctcgaca tcaacttctg cccgcacgat gcccaggagg atgcggaagc
     1501 tgcgggtatg atcctggttc gagcaatggc agaaacaggg atgggaatgg cggagtggat
     1561 ggagcgggtc aagaagccca tagacccgac tcgcccctca tccggcccta tccacctcga
     1621 tggcaaccca gagggtgagt tctatggcga agtgttggta ttcacgggtg ccctatcgct
     1681 cccaagagtg gaggcagcta agctcgcggc tcaagccggt tgtcacgttg atgatggtgt
     1741 caccaaacat accactctcc tagtggtggg agatcaggac ctgacgaaac ttgccggcca
     1801 cgagaagagc agcaagcacc gcaaggctga gtcgctgatc gccaaagggc aacccattcg
     1861 gatcctggcc gaaagtgact tccgactgct ggtcgggatg agctaaggcc tacatagctc
     1921 gtcaagaggt gtgcatatag tacaggaagg agactggcgt gtagacgaaa gtccgctgcc
     1981 ttgatttcgg cccatctatc ccaacgattg agtaggagga gtagccgtga gcaagaaacg
     2041 gtggaatagg tttgcaccac gccccatcca gcgagagttc cctgggaagc agtctaggag
     2101 atcactaccg gttccgagtt actcgcagcg aatgtagctc tccattcgag gcgattgccg
     2161 gtacaggccg cccagagatc gggggcggcc tgtaccggcc caatggttat cgaacagggt
     2221 caaacgcaca ttctagtaag agtcgccgca gatgacactg ccgagacgaa cgagcgcgtt
     2281 cctggccaat gattgagcgt tatgcccgga gggatctgat tgcaggcccc cattctgtcg
     2341 gcgcccaata ggaaatacgc aggcctttcg aagaatactc agtatccacc gttgaaattg
     2401 cggtggtggg cgtttgccgc tctgcctggc ctttctatga ccattgctta ccacctcttc
     2461 ccacaactgg ctgtggcacc cttcggaaca gcactgtggg taactggagc tgcactgctt
     2521 ctcccattcg tcatgagcgt cctgcaatat ctcgccgaga tagctcgtat tacgatattt
     2581 cgccttcgtg cctatccctc gctggaaggc gatgcacgcc aactgtctgc cgacttgcaa
     2641 gccgaacgca gcaggctcag tagcttgatg gggttgatcc cggaggaagc accgcgccat
     2701 gagtttcgcg ccgttcggcg agacccaatc accggtgagg cgtatttcat cttgaagaag
     2761 aacgaggcag tctcattgaa tgagggtgac tctctccttg ctgtagatcg atatgacagc
     2821 caattgatgt gtagcttgcg ggtcgtgtcc atcgaaatgg atagctatcg ggtgcaagtt
     2881 acggacgtta gagatgcgct ctggtgggga tatatgcaag tccaagctga tttacacctg
     2941 catgcgccgt tggaaacgga tacggtggca gtgttcgtac cgctcaacaa ggagaacagc
     3001 catgaatgag aaaccattag aaggcaaggt agcgcaaatc ctcaatgagc gcgaacttgt
     3061 catcaacatc ggatccgctg ctggtgtcat taggggcatg aggttcgccg tgctagccgc
     3121 aactccggtg gacgttaagg atcccgatac cggcgatgtg atcgacgtag ttgaccgagc
     3181 gaagactcat gttgaggcca ctgaggtcag ggagagaata accatctgtg gaacatacca
     3241 ccgtcgctat gtcggcggca gcatccaaca ggtgctgaaa gaaatggagc aattcagtgg
     3301 ttggcaacgg caaacgttca ggataggaga tggtggactt cccaagccga tcgacccgac
     3361 ggaaagctac gtcaagatcg gagatagggt caaactagtc atcgagccaa acgaagagta
     3421 atccaatcag ccagacaagg agggtcttcc ggacgagaag aggcggcaag accaaagctt
     3481 tgccatcatc gcccattgac gtatgggaag gatcaagcca tggcatgcag gagccccggc
     3541 gagggcagcg tcttcaaacg caagaaagac gggaggatcg tcgtccagat cccgctaccc
     3601 gaaggcggca ggcaggcgtt ctataccaag gaggacggga cagtcttcaa gacgaagcaa
     3661 gaggggaagg acttcctcag tgaacattcc tcagtgaaca gatggccaag ctgaagggag
     3721 gcttgcccct cggcccaggg gagccattgg ccacggtcct agccaggtgg gtggaggtgg
     3781 tggtggcgcc gcagctggat gcgtggacct atgaggatta tgagcccctc ctccgcctgc
     3841 acgtgctgcc caccctgtgt gacatgccgg tggggcgact gaccggccag cacctcgaga
     3901 ctctctatgc caagaagcgt actgagccac aggataagcg aacggagccc ctattcgagc
     3961 ggcggatcca gcaatccacg cagtgcccca tacctcgctc aagcacatcc acaacaagtg
     4021 gaagctgatt tcctgcaacc ccgcggagga cgttgacgcc ccggtcccca ccaagcgccg
     4081 agcgctgatc ctgaacgtcg gccgggcgaa gcagctcctg accaggccca gggcgatccc
     4141 cttgaggcac tctacgtatt ggccatcact acgacgatgc ggcagggtga gttcctcgga
     4201 ctacactgga aagcgatcga cttcgaggag gggctccttc acgtcatgca gaacgtgcaa
     4261 cgcaggaaac gagagtggag attgaaggat ctcaagactg acagttgtcg acgccccatc
     4321 ggactcgcca ggatggctat cgcagccata tgtcagcacc gggcgcgcca ggaggaaatc
     4381 agaacacgtg cagacggaac atggcaggac atgggtctcg tgttctgcga cgtgaccggt
     4441 ggcccattga aggggaagaa tgtcaccgtg aagtacttct acccccttct caagaagacc
     4501 ggcatcgaga tagaggactt tcactttcac gacctgcgtc acaccgctgg atccctctat
     4561 atggccatgg gggtctcgcc aaaagtcatc caagagatga tgggacactc aactgttgcg
     4621 atgactttgg acacctacag ccacccattg cccgatcttc aggcccggca gtggccaaga
     4681 tggacgaact cctcggatct gcactcctcg aagagtaatc cggcaaaggc caaaactcac
     4741 aagatgccac gcctcgcgta ctcagggccg ataccttcat caggttcaca tgcctggagc
     4801 acgtatcgcg tgacgaagca ctcattccaa ccctggatga agaacggttc gcaaggatcc
     4861 tgggcccact caatctccgc ggcttcttct gggtcatcct gcaggtattg ctccgagagg
     4921 aatgccttcg ctctgtcaac gctggtgaag atcccaatgc atggtccccc atcctcgggg
     4981 ccaatccctt gtccgtcgca ctgactactg agcacccaga gccgcatttc cccctccatt
     5041 agcacacaac aactcgccct tggtgaggta atccaacact tacaatcggg tcctcgtagg
     5101 gctcagagac tctgctccct cgcacgttgc tacattcgtt gctacatcga agtgtttccg
     5161 atcgttgttc gcataccttc tgaccgataa acgagcaaca ggtataacaa tcgtgagcac
     5221 cattgctatc cctgtagtct ggtaccgggg aggggactcg aacccctact cctttgcggg
     5281 aagcggattt taagtccgcc gcaagccgaa tttcgtgcct gtttggaccc tctgacaccc
     5341 ttctgacacc tttttcgcac cgtcggggac atagcgagga ggagaggcta tgtcccggca
     5401 gcaggagcgc acctacggcg tgggttcggt ctaccagcgg aaatctgacg gccgttgggt
     5461 ggccaaactg ggcaacgacg gcaagcccat caccagatac gccgcaacgg agaaggaggc
     5521 caagactatc ctggtcgcac tgcaacggga gtgcggccag aacggtactc tcgccacccc
     5581 cggcaacagg accgtcaacg acctgttgga cctgtggctg cagaccgccc agcccaacct
     5641 gaagccccgc accttccacg actaccaggc ccggtgcaaa cgccacctcc ggccagttct
     5701 cggacacgtc aaactcagca agctctcccc cgaacgggtg caggctttct acaccaagct
     5761 gctgtcctgt ggtatcgaac gagaagccct ctacagccat gccatgctgc accgggcctg
     5821 caagatggcg gtgctgtggg ggtggctggc ccagaaccca tgtgaccggg taatccgccc
     5881 caagcaccag gccgaacgga aggaaatgtg gagcatagag gaactgcacc ggttccttga
     5941 gggaaccagg gaccaatggc tgcatccgct gtgggtagtg gcagcctcca ccggttgccg
     6001 actgtcagaa ctgcagggcc tgcgctggca ggacgtggac cttgcggcca gcacactacg
     6061 tgtatcacgc tccctgcacc gcattgaagg tcaatgggtg gagtccactc caaagaccag
     6121 ggcgggaaac cgaacgatgg tacttccagc cgcagccgtt gccgcattga agggacagag
     6181 ggcgcaacag gcagagtggc ggctgaaggc tggcccccaa tggcaggata cggccctagt
     6241 gttcacgggt gagcacgggc agcctctcgc ccagtcaacc gtacagaagg ccatgcaccg
     6301 agagattgag cggttggatc tgccgccgct cacgccccac gggctacgcc acatggcggc
     6361 ttccctgctt ctcagcaagg gcctccctgt tccggcagta tcggcccgtt tgggtcacgc
     6421 cagccccaac atcaccatga gtatctatgc tcacgccatg aagcagcagg accaacaggc
     6481 agcagcactg atgcaggagg taatgaatag cagggcatag aaggtagagc tctatatgct
     6541 ggccgttctg gctttcgccc ggctgtatgc ggtctgcagc gcattgggtc gggtgaaagc
     6601 cctctcggga tgcatgtcgt tccacgttga taggagggat ttccagcttc tttttgctcg
     6661 tgatgagggc cggggagcca ttgaagcctc aacgaagcga actagctcaa ggtctgctgg
     6721 agttgagcct cgtttccgat atgctccagt ccgctctctc atggtgcggt gtagggctct
     6781 ggtctcacgg tagttgagcg ggccgtagac ctcgatcagg gatcggacac cacctcccgg
     6841 cagtctggat gtccagagcc taacccgtgg cacctgcggg acttggttag taaggagaaa
     6901 caaggttgcc tgccccaaac tccaacagcc agagcgcttg gatacgacct tggcggccct
     6961 ggatagctgc ttcaagcaat gttgggtgac cttgtactca acctcgccgg gcaatacagg
     7021 gttcgatagg tccaggtaaa gtctctcgac ttggtcgtcc ttggctgtct caacaggcgg
     7081 aagataaacc atttcgaagt ccggtccggt cggcaccacc ttgaggctcg gctcgatctg
     7141 caccgcttcc ccacgttcca cctcgccaag tgcccggcgt gcttccgcct caaggtcaat
     7201 gctgttatcc ctcaaccacg catgcgccga cataaggtcc ggaagggctg gccaggaccg
     7261 ccgccacggg tcaaccatta tcttcaagaa gtattggaac tgcgggtcct cgacgacgga
     7321 ttgggatatt cgcccaattt gagcttgggc aggccactcc cagtctagtt gccattgcct
     7381 tgcatgctcc tccagccggg gtggaagcgt cctcgcaatg tcatccggaa cttcctcatc
     7441 ttcgggccga tagccttccc gctcctcttc actgagctct ctaccgaaga actcctcgat
     7501 gatctctctg tgccccacag gttcctcctc cgccatagtc tcacttaact gtgcatactg
     7561 agactatctg tatggtatgc gcagcagaag aagaagtcaa cttgtctgga aattagtgca
     7621 gatgtggagg aacgaatgag aaccatttcc ccaaacccaa ctctccgccg cttgcgagag
     7681 gaaagcgggc tgacaatctg gatgctggct gtggtctctg gcgttgccgc cagcaccatc
     7741 tctgccgcag agaagattgg gtacaagccc ggacaaggag ctaggtcaag gctagctgca
     7801 gcgctcggtg tcacgccgga gtcgatctgg ccagagtgtg ctacgaagga ggaagaacgg
     7861 caatgacaac tctcggtctt cgccaagaag acttctcgcc gtttgccctg gcgatcagga
     7921 aaggcgggca atggcgaaca ataggtaagt tagtgcttgc caagccgctc gaagagatcg
     7981 tgctcgcctt cccgtaccgg aatcgtgttt ctctggatca cgtcagcgct ccggtctcgg
     8041 tgctgaagca cgcccaccat gaaggcgcca agtggtgggt tgttcgcctc gaccgggagg
     8101 gcatctgcct aggtcttccc atggtgatga tccccaaggt agggttccgg ctcgatgagg
     8161 gagactcgtg tgaaatagcc gtcgaacttg ccgatttcga gccaatcgat tggcaggact
     8221 ggccctacgt tgaacggctt gtccatttcc cgctggaggc aacccatgcc tgacgctacc
     8281 agactaaagg ccattcaggg ctacctgaag cggggttggt acgtgatccc cctttgttgg
     8341 ccatctgctg gcgagtgcgg atgtggcagg ggacacacag gcaaggagat cgggaaggct
     8401 ccgctgcttg ggtcaggcta ccagaaccgt cgatcaacag aatctgacgt caatgaatgg
     8461 tggacccggt ggccagatgc gaacatcgga atactgttgg agccgagcgg actcctcgtg
     8521 atcgactgtg atggggttga agccttgcga gaggccaggc tctttggact tcccgaaggt
     8581 cctgtggttc gaagtgggaa tggcgttcac atctactaca aggccggtga tgcaactgga
     8641 cgtgccacga agcaagggaa ctctcacgcc attgacgtgc tggcatcggg tttcgttgtg
     8701 gctccgccca gtcgacaccg gaacgggcat acctatgagt ggcggaggag ttgagcacag
     8761 tgttgcagga tgcgcccatg tgggctgttc aagtgctgac gaagacgcct tccgctggtc
     8821 cgtcgcccag aatagccgtg gacagcttgc cacccgttcg cttaggcgac aagggtgtca
     8881 ggctctggca gggggagttg gctgtggaca aggccgacgg caggctcaag gcctccgacg
     8941 tcgtctccga gatcgatact tcggaaaccc tgtgtgccct aggcggcgag ttggccaaag
     9001 caggggccgc tgagaccacg atacgtgcag ccttagcaga gcgggaccgt gtgctcggct
     9061 accacaagta cgacgcacga aaaggtgggg ggctagagga gtacgctcga atcgcagcga
     9121 aggtcacaac cgagaagcct atggtcgtcg atctgcacac tacggacatg ggcaatgccc
     9181 agaggttggt ggctctccac ggtgcagacc ttcgttattg ccaagctaac agacaatggt
     9241 acttgtggga tgggcggcga tgggcgccgg acgggacagg ggaggtatac cggcgggctc
     9301 aagacaccgt tgcggtgatg tatcaccaag ccctgaagga gcctgaccac gacagaaagg
     9361 aagctctgct caagtgggct ctgcgttccg aggggagaga ccgcatagag gcgatggtgg
     9421 cgctggctcg ttcttcccaa gaaatggctg taacgcctgc tcaactggac gccaatcctt
     9481 ggctgttgaa cgtgatgaac ggcacgctgg atcttcggac cggacacaca agacccccca
     9541 atagggaaga cctgataacc aaactggcgc cggtccagta caaccgggat gcgaacctgc
     9601 cgctatggga ccgtttcatc accgacctga ccaacgatga cccggagctt gctgcctttc
     9661 tccaacgagc ggtgggctac agcctcacag gcgacaccag agaggaagtt ctcttctttg
     9721 gtcatggacc atctgctaca ggcaaaagta cctttatcga ggccgtcaag gcaacattcg
     9781 gcgattacgc tagcacggcg gatttcgaga cgtttttagc tcgaagggat gccggtggcc
     9841 caagaaacga catcgccaga ctcggtggct ctcgcttcgt ggcctccgtc gaggtggatg
     9901 aaggcaagcg actcgctgag ggcctcatca aaatgctaac cggaggcgac acagtatccg
     9961 cccgattcct gtaccaagag acgttcgagt ttcgaccccg cttcaagctc tggctcatgg
    10021 ccaatgatcc acccaaggtg agagacaacg atgatgccct ctggcgacgg attctgcggg
    10081 tgccgttcgt tcattccgtt ccgaaagatc ggcgggaccc ggccgttaag gccacgctac
    10141 gagatcccaa gactgccggt ccggcagtgc ttgcctgggc tgtcagggga tgcttggagt
    10201 ggcagaaagg aggcttgaag gttccccaag ttgtggagca ctcgaccgag acctaccgac
    10261 aggaaatgga tccgctgcgg ctcttccttg atgagcggtg caccttggat cagggtcgat
    10321 ggacaccgag tgctgaactc cgtagcgcat acgagacgtg ggccagggag tccggcgagc
    10381 cagacatttt gtctgggagg gcattcgcag agcggcttag ggctttgggc tgtacctcta
    10441 ccaagaaacg agtgcagacc acaacgaagg acaggagcgt ctctacatgg ggctggcaag
    10501 gcatcggcct tgtcgaggta tgagctgttc cgtgttccgc aatgtactgc cgttttccac
    10561 aacttcttac ttagtctctc cataagggaa agtcaccgaa aatgacggaa catcacggaa
    10621 cacggaacag gatcactcag agactggacc aagtctaccc aaatagaaag gaatagagac
    10681 gtggcagctt cgaggaagat gcacgcagac ccagccatgc tgaaactgct ggagaagcag
    10741 gtgcttcaga taatctcaaa acccaggaat tggtggtcgc cccgtggggg gttctggctg
    10801 gacgaaatag gttgggggac acttcgggcc tacggtctca agagaagtga ggctgaggcc
    10861 gccatcgaca gcctatggaa caagggaatg atcacgatct caccagaaga gggcaatcgt
    10921 gtggtacgag ttgccaaacc ggcacgagcc agggacgatg ccagtatcga ggagttgctt
    10981 gatgacttca ttccctagct tgacgccgaa atgggctagt ttccacccat cagtgtcccg
    11041 atgcagcggg ttggaggagc gggtagcctc cgctcctcca ctcaacgggc aaaccaaacg
    11101 ctacattctc aatggcattc ggccgggctc tcttggccgt tgggagaccg tgcaagacgt
    11161 agatacccaa gtggtcactg ctctcaggca agcaaatttc actgcagagg aagtgcaatc
    11221 catctaccag tcattcccta tcggcacccc caagcgggat tctgcctacc tggctcgtct
    11281 tgtcgaagac gcagaccgaa agattgcccg aactgaggcc gaagctcgcc gtgtgcgggg
    11341 gctcaacttc gaggtggtca aggtggtcca cgtcaacggc ctatacccag cctacttgtt
    11401 gacggtcacg ctccaagatg aatctgaaca cacagtgtca gtcgctcatg cggcgataac
    11461 ct
//
LOCUS       DATALS010000560         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_83922,
            whole genome shotgun sequence.
ACCESSION   DATALS010000560 DATALS010000000
VERSION     DATALS010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83922"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VHS06_12520"
     CDS             <1..1024
                     /locus_tag="VHS06_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2988976.1"
                     /translation="ETALRPMGTGPWRVTDWAKGDHLTLQSFPGYWRGDSGIPEFSFK
                     PVPEKGTRLNMLLAGEADIVGGLTPEDFPSIEGNSKLRMSRAPGNRRVHLGIPTQVPR
                     YKNRAVRQALLQAIDYDGIAKALLGPMAPKPRSNVLVAGDSWTNPSLQPVAFNPDAAK
                     KGLQAAGFPFSEKVTIYVPGGRYLKGNEVAQAVAGNLRNIGINADAQILDWTVYSDKM
                     RSDKGLDDLYVLGLGSRFNGPEDVSIITTGQVWDQTQWIKNTENGPKFEKMYKDLTQT
                     FDEKKQHELINEMQRLFMEEAIWGSLWLEPAASGVTKRLNWEDSGGGNRLRFWLANED
                     PTKVSG"
     gene            1150..2067
                     /locus_tag="VHS06_12525"
     CDS             1150..2067
                     /locus_tag="VHS06_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2988977.1"
                     /translation="MLPYIIRRLAQTVLVLLGVTALSFGTMFLSGDPTMLMAGETWTV
                     EQVNEFSHQMGFDRPWYVQYGDFLSKAVRGDFGVSLRQQQPTFSLVMERMPNTLQLAA
                     AALAIALCFGLPVGVLAALRRGSIWDNMLMLGGLLGQSLPVFWLGLLLIMVFSVGLGW
                     FPVAGIGTPRHLVLPAVTLGLFSTAYVARVARSSMLEVLGRDYVRTAHAKGLPPRAVI
                     LRHALRNALIPLVTVIGLQAGQLLGGAVITETIFAWPGVGRLTIQAVQGKDLPLVQAC
                     VTLLAANFVFINLLVDLLYTYLDPRIRLK"
     gene            2064..2957
                     /locus_tag="VHS06_12530"
     CDS             2064..2957
                     /locus_tag="VHS06_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2988978.1"
                     /translation="MSASSVTRLATSEMPGIAHRRRRFARLRRMRLAGVGAVILLLVV
                     AVAVAAPWIAPYDPAKVNLIDRLKPPLSPGHLLGTDSLGQDVLSRLIYGARVSTLVGF
                     LAVAIAGTLGVTLGLLSGYYGGWLDDIIMRIAEIQLAFPTIILYVAVMAVLGPGLMKV
                     IAVIGVVGWVQYGRMERGMVLSVRQQEYVEAARALGARDRVLLIRHILPNTLGPMMVV
                     ASFGLATTIITEASLSFLGLGVPPSVPSWGSMLADGRDYLRSGWWVATFPGIAITLTV
                     LAINLLGDWLRDELDPLLKVQ"
     gene            2998..>4008
                     /locus_tag="VHS06_12535"
     CDS             2998..>4008
                     /locus_tag="VHS06_12535"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX2988979.1"
                     /translation="MFRNADVSVSGQGKVLYLKGTPYEQGRQLGRGAADLIRANIDRA
                     AKLRDEVAAGRDQANYHAITRMNEQWVSREFPELLEELTGISEGAEVDHQELLNLNLN
                     THIAYIYSTMLSCTQALATGPATVDGKTYVGKTRDLSRGPLLQLLLHREYPDGSYLNE
                     IQTAGRMTIPDGINQWGVSLTCSGQWSPRVTVDLSRADSAWLTLNLQPVLRMARTADE
                     AVQMIKEQPRASGMHVLVADGSKAVALEVTDQVVREFEAVDGLLVRTNHYFAPDLQHL
                     VPTFEENRSSYDRFARATEILRQRHGQIGMHDILRVVSDHSEPPIESICRHGDGDLQS
                     KTC"
BASE COUNT          708 a         1198 c         1331 g          771 t
ORIGIN      
        1 ggagacggcg cttcgcccca tgggtacggg gccatggcgt gtcaccgact gggccaaagg
       61 cgatcacctg acgctgcaga gcttcccagg atactggcgc ggggattctg gcataccgga
      121 gttcagcttc aagccggttc cggaaaaggg cactcggctg aacatgctgc tggcgggcga
      181 ggcagacatc gttgggggac taaccccgga ggacttcccg tcgattgagg ggaacagcaa
      241 gctgcgcatg agccgtgcgc ccggcaaccg gcgggtgcac ctggggatcc cgacacaggt
      301 gcctcgctac aagaatcgcg ctgttcggca ggcattgctc caggccatcg actacgacgg
      361 gatcgccaag gcgctcctgg gccccatggc gccaaaaccc cgctccaacg ttctggtggc
      421 cggagactcc tggacgaacc ccagcctcca gcccgtcgcg ttcaacccgg acgcggccaa
      481 gaaggggctc caggcggcgg gcttcccctt cagcgagaag gtgaccatct acgtgcccgg
      541 cggccgctat ctgaagggga acgaggttgc ccaggccgtc gccggcaacc tgcgcaacat
      601 cggcatcaac gcagacgcac agatcctcga ctggacggtg tattccgaca agatgcggag
      661 cgacaagggg ctggatgacc tctacgtgtt ggggctgggc tcgcggttca acggcccgga
      721 agacgtcagc atcatcacca cggggcaggt ctgggaccag acccagtgga tcaagaacac
      781 ggagaacggt cccaagttcg aaaagatgta caaggacttg acccaaacct tcgacgagaa
      841 gaagcagcac gagctgatca acgagatgca gcgcctgttc atggaggagg cgatctgggg
      901 atcgctgtgg ctcgagcccg cggcgagcgg tgtgaccaag cggctcaact gggaggactc
      961 cggaggcggc aacaggttgc gcttctggct cgccaacgaa gatcctacca aggtgtccgg
     1021 ctgagactcg atctcagcag tcttgatggt tagcggagag ggctttgccc ggtcctgccg
     1081 gctcgggctg aagccctctc tccagcgccg ccagttcctc gtggtagagc tcttgctggg
     1141 gtgacaccaa tgctccctta catcatacgg cggcttgccc aaacggtgtt agtgctgtta
     1201 ggcgtcaccg ctctatcctt cgggaccatg tttctgagcg gcgacccgac catgctgatg
     1261 gccggcgaga catggaccgt cgagcaggta aacgagttca gccaccagat gggattcgat
     1321 cggccctggt acgtccagta cggtgatttc ctctcgaagg ccgtgcgcgg tgacttcggg
     1381 gtctcgttac gccagcagca gccgaccttc tcgctggtga tggagcggat gccgaacacc
     1441 ctgcagctgg cggcggcggc ccttgccatt gccctctgct tcgggctgcc ggtaggtgtg
     1501 ctagcagccc ttcgccgtgg gagcatctgg gacaacatgc tcatgttggg cgggctgctg
     1561 ggccaatctc ttcccgtctt ctggctaggc ctgctgctga tcatggtctt ttccgtaggt
     1621 ttgggctggt tccctgtcgc tgggatcggc accccgcgac atctggtgct gccggccgtc
     1681 accctggggc ttttctccac ggcctacgtc gctcgtgtgg ctcgaagcag catgctcgag
     1741 gtgctcggcc gtgactacgt gcgcactgcc cacgctaagg ggctgccccc gcgagcggtc
     1801 atccttcgcc acgctcttcg caacgcgctg atcccccttg tgaccgtgat cggcttgcag
     1861 gccggccaat tgctgggcgg tgccgtgatc acggagacca tcttcgcctg gccgggggta
     1921 gggcgattga ccattcaggc cgtgcaaggg aaggacctgc cgctggttca ggcctgcgtc
     1981 acgctgctcg cggccaactt cgtgttcatc aacctgctgg tggaccttct gtacacctac
     2041 cttgacccaa ggatcagact gaaatgagcg cctcaagtgt gaccagattg gcgacctcgg
     2101 agatgccggg gattgctcac cgtcggcggc gcttcgctcg cttgcgcagg atgcggctgg
     2161 cgggagtggg ggcggtgatc ttgttgctgg tggtggcagt cgctgtagct gctccctgga
     2221 tcgcgcctta cgatccggcg aaggtgaatc tcatcgatcg tctgaagccc ccgctgagtc
     2281 ctgggcacct gctggggacc gattccctgg ggcaggatgt gctcagccgt ctcatctacg
     2341 gtgctcgtgt ctcgacgctg gtgggcttcc tggccgtagc tatcgccggg accctgggtg
     2401 tgacgctggg gctgctgtct ggctactatg gcggttggct ggacgacata atcatgcgca
     2461 tcgccgagat ccagctggcc tttccaacga tcatcctgta cgtggccgtg atggcggtgc
     2521 tggggccagg gctgatgaag gtgatcgcgg tcatcggagt cgttggctgg gtgcagtacg
     2581 gtcgcatgga gcgagggatg gtgctctcgg ttcgccagca ggagtatgtg gaggcggcca
     2641 gggcgttggg ggcaagagat cgggtgctgc tgattcgcca catcctgccg aacacccttg
     2701 gtcccatgat ggtggtggcg agcttcggtc tggccaccac catcatcact gaggcaagtc
     2761 tcagcttctt ggggctgggg gtgccgccgt cggtgccttc ctggggaagc atgctggcgg
     2821 acggccgcga ctaccttcgc agtggctggt gggtggcgac attcccgggt atcgcaatca
     2881 cgttgacggt tctggccata aatcttctgg gcgactggct acgcgacgaa ctggatccgc
     2941 tgttgaaggt tcagtagaag tccgcgagca cgctggagag aggagatctg ttgatagatg
     3001 tttcgcaacg ctgacgtctc cgtgtcgggg cagggcaagg tgctctatct gaaggggaca
     3061 ccgtacgagc agggccggca gttgggcagg ggggcagccg acctgattcg ggccaacatt
     3121 gaccgtgctg cgaagctgcg agacgaggtg gcggccggcc gtgaccaggc caactaccac
     3181 gccatcactc gtatgaacga gcagtgggtg tcccgggagt tcccggagct gctggaagag
     3241 ctaaccggca tctccgaggg tgcggaggtg gaccaccagg agctgctaaa cctgaacctc
     3301 aatacccaca ttgcctacat ctactcgacg atgctgtcct gcacccaggc gctggcgacc
     3361 gggcccgcca cggtggatgg aaagacctac gttgggaaga ctcgggatct gtcacgcgga
     3421 ccgttgctcc agctgctgtt gcatcgagag taccccgacg gcagctacct caacgagatc
     3481 caaaccgcgg ggcgaatgac catccccgat gggatcaacc agtggggcgt ttccctcacc
     3541 tgcagtggcc agtggtcgcc acgggtgacc gtggatctgt cccgagccga ttcggcatgg
     3601 ctgaccctca atctccaacc ggtcctcagg atggcgcgca ccgctgacga ggcggtacag
     3661 atgatcaagg agcagccgcg ggccagcgga atgcacgtcc tggtggcaga tggcagcaag
     3721 gctgtggctc tggaggtgac cgatcaggtc gtgcgcgagt tcgaggctgt ggacggcctg
     3781 ctggtccgca ccaatcacta ctttgcccct gacctgcagc atctggtgcc gaccttcgag
     3841 gagaaccgga gcagctacga tcgtttcgcc cgggccactg agatcctcag gcagcgccat
     3901 gggcagatcg gcatgcacga tatcctgcgg gtcgtgagcg accattccga acctcccatt
     3961 gagagcatct gtcgccacgg tgacggtgat ctgcagtcga agacctgt
//
LOCUS       DATALS010000561         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_83932,
            whole genome shotgun sequence.
ACCESSION   DATALS010000561 DATALS010000000
VERSION     DATALS010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83932"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VHS06_12540"
     CDS             complement(<1..884)
                     /locus_tag="VHS06_12540"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX2988980.1"
                     /translation="MGSGIKRIGVLTGGGDAPGLNAVIRAVVRTAINKYGWEVLGIAD
                     GFEGLLFQREPRQLTLQDVRGILHLGGTILGTTNRGNPFAYRVQVGGKTQVRDLSRDT
                     LARIRDLGLDALLVIGGDGSLKIAHELALMGAPIVGIPKTIDNDLSATDVTFGFDTAL
                     HTATDALDKLHTTAESHHRVMILEVMGRDAGWIALEAGLAGGADVILIPEIPYRLESI
                     EKCIARREAAGRTFSLVVVAEGAMPADGRQVFQEDATEDKLARLGGISSHLATQLARR
                     IPQDVRFTVLGHLQRGGSP"
     gene            1109..1792
                     /locus_tag="VHS06_12545"
     CDS             1109..1792
                     /locus_tag="VHS06_12545"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX2988981.1"
                     /translation="MPVFAESEIGGITGEVGVVDVGLDPGHSDADVGASGGGLRECDL
                     TLEVATRVKSLLEEEGLSVVLSRTDNRPLTDFSNPDPTEQIRLEQEARVAAVGRARVF
                     VSIHFNGYGDPSVRGAECYYNGDNQGEPSQRLGRGIQNKLLAEVTAAGYDLPDRGVKE
                     DLSAGKPYGHFFSLRGDMPSVLVEAMFLTNPHEAFLVGDKSIREAVARGIASGVWQQI
                     SSSHRPIEP"
BASE COUNT          331 a          551 c          574 g          384 t
ORIGIN      
        1 gggcttcctc ccctctgaag atggccgagc acggtgaacc tcacgtcctg gggaatcctg
       61 cgagccagtt gggttgccag atggctggat atgccgccaa gccgtgcaag cttatcctcc
      121 gtagcgtcct cctggaagac ctgtcggcca tccgccggca tggccccctc agccaccact
      181 accaggctga aggtgcggcc cgccgcttcg cgtcgagcga tgcacttttc tatgctctcc
      241 agcctgtagg gaatttccgg tatcaggatc acatcggcgc ctccggccag cccggcttcc
      301 agcgctatcc aacccgcatc ccttcccatc acctccagga tcatcaccct gtggtggctc
      361 tcagccgtcg tgtgcagctt gtccagcgcg tccgtggcgg tgtggagcgc ggtgtcgaag
      421 ccgaaagtca cgtcggtggc gctcaggtcg ttgtcgatgg tcttgggtat tccgacgatc
      481 ggtgcgccca tcagtgctag ctcgtgagcg atcttcagcg acccgtcgcc acctatgacc
      541 agcagggcgt ctaaccccag gtcgcgaatg cgggcaagtg tgtctcggga caggtctcgc
      601 acctgggtct ttccaccaac ctgtaccctg taggcgaagg ggttgccgcg gttggttgtt
      661 cccaggatgg tgccgcccag gtgcaatatt ccccttacgt cctggagggt tagttgcctg
      721 ggttcccgct gaaatagcaa cccttcgaag ccgtccgcaa tcccgagcac ttcccatccg
      781 tacttgttga tcgcggtgcg cactactgcg cgtatgacgg cgttcagacc gggcgcatcc
      841 ccaccgcctg tcagaacgcc gattctcttg atcccgctgc ccatcgacct ctacctcacg
      901 cgaatattgg tggagccacc ggcaccgttc tttacatagt atcggcagtt cttctcatta
      961 tcttcacctt gactggtgga ggttcttcat caagatgata tcatgggaaa ggcaggactc
     1021 catacgagag ccctagggaa ctccgatcag ggaggaagtg gtgctcaaat cggccttttt
     1081 ggcaggcctg atcttggtgg cttcctgcat gcctgttttc gcggagagcg agatcggagg
     1141 gatcaccggc gaggtcggcg ttgtcgacgt gggattggac ccagggcaca gcgatgcaga
     1201 tgtgggtgca tccggagggg gcctgcggga gtgcgacctg acgctggagg tggctaccag
     1261 ggtaaagtcg ttgctggagg aggaaggcct ctccgtggtc ttgagccgca ccgacaatcg
     1321 tcccctgacc gacttctcga atcccgatcc caccgagcag attcggctgg agcaagaggc
     1381 ccgagtcgcc gcggtgggaa gagctcgggt tttcgtgtcc attcatttca acggctacgg
     1441 cgacccatct gtgaggggcg cggagtgcta ctacaacggc gacaaccagg gtgagccgag
     1501 ccagcgacta ggaagaggca tccagaataa gctgctggcg gaggtgactg ccgcgggata
     1561 cgatctgccg gaccgcggcg tgaaggagga tctctcggcg ggcaagccgt acggccactt
     1621 cttcagcctt cggggtgaca tgcccagcgt tctcgtggag gcgatgttcc taaccaaccc
     1681 gcacgaagcg tttcttgttg gggataagtc catccgtgag gcggtggcga gggggattgc
     1741 gtcgggcgtg tggcagcaga tcagctccag ccatcgcccg atcgagccgt aaccaaattt
     1801 gtggagtgcg ggcatctcac caagtagggc gggtcagcaa
//
LOCUS       DATALS010000562         6364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_84031,
            whole genome shotgun sequence.
ACCESSION   DATALS010000562 DATALS010000000
VERSION     DATALS010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84031"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1780)
                     /locus_tag="VHS06_12550"
     CDS             complement(<1..1780)
                     /locus_tag="VHS06_12550"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX2988982.1"
                     /translation="MQTAPKHGRGHSRIGDLSVGIKITILCVGLCAALAIALTAIGYR
                     QASEGLRTQAESALASDAMTVASTVDGWNIQRIRDLQSLSANPVIQEFAAADPVARAT
                     QMKQIQGALKSLLDSASDEGLEAVGIMDSAGNFVADTASTLPAPSPQRDYFKAAMNGK
                     AFISGVSISTVTNAASIYHSTPIKGVDGRVIAVLRSRAGLAPLQRAIEAAKSRMGKQS
                     TGVLLDESGLVVASDVDAGWVMRPVIPLKPEILQPLVQEKRWGNNASPDPLGQTDLSG
                     AIGAKKSARFNWKLQGKPFDAMVVPLTQTTWSYVEALPLEVVEAPANSFLQGSAIAAL
                     VALLLATFFAVLFARSLASTARHLAEGARHIASGDLDYEIKVSTGDEIGRAALAFKDL
                     AASERALAETAQAVASGDLTRDVVARSESDVLGLAFRRMNASLRDLVGRVQSSVETLA
                     CSSTELGTTASQTGAALQETESTIQGMARGSQEQSEATEITSANMVQLSRAIDQIATG
                     SQEQARSIEKTSASVAQLNLSIARVAGASEEVSLATQQARAAAASGADSVGKSVKGMQ
                     AVQASTTDVAARIAKLGSNSSQIGVIV"
     gene            complement(2035..4491)
                     /locus_tag="VHS06_12555"
     CDS             complement(2035..4491)
                     /locus_tag="VHS06_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX2988983.1"
                     /translation="MASKTRGRDGEKLGFLQSFKGKFFIIVLLLAILPALVVGLVAGR
                     QSGSALETEASEKLQETAQLNAQRTQDWANTRLKEVQVLAETDSVRSMDPAIARPFLK
                     TAQKQFGGTFATFSLVNGSGTIIANTEDQEGASVTDRDYYKPAMSGNPVISDALVSKA
                     TGDIVVIAVSPVKTKDGKTGGIILGTVSLKGFYEQNLAKGSMKDSYTFVLSKDGTTIA
                     HPVADNIMKDNPTKATDPAMAAMAKAMVQGQTGTANYSYNGAGKVAGYGPVGVNGWSV
                     ATVQGRDAALGAVDEINRISWSILALVAVIVIPLAIWIARSFSSPITFLAAAAIEYAE
                     GGLGQDLDAGTVERMLRRRDELGLLAKSFKRMANGFKGMADVARRIADGDLTAQVELR
                     SDHDVLGIAFQEMSSHLREMVAETQSSARELAATSTQLGEAANQTGDAVQQVNVTIQS
                     VAQGAQQQATVAQETTSAMSDLTQAISQVASGSNDQARGIQSANDTAGQMAVGVEQVA
                     ANANQVAHESEKTRDAAVRGNQMVQETIAGISRLKETVDRTATQIDELGRASEQIGAV
                     VETIDDIAEQTNLLALNAAIEAARAGEHGRGFAVVADEVRKLAERSSKETRQIAELVR
                     SVQAGTKAAVDAMHSGASEAEAGSDLAARAGEALEQIVRSVEETVQQVSQIAAASQQM
                     AASSKGVTAALETISAVTEQSLAATEQMELLARTVSQAIESSAATAEENSAATEEVSA
                     SSEEMSAQVEEMSAQAAQLSRTAEELRKMVARFKLDESDGGQAGLARPSEVVVQKRRT
                     DDWRSIPERQGGNAGTLSRP"
     gene            complement(5035..>6364)
                     /locus_tag="VHS06_12560"
     CDS             complement(5035..>6364)
                     /locus_tag="VHS06_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX2988984.1"
                     /translation="LLVAVKGAPEAVIARCSRQVVEELESPFGEQPRTTAFSAAEAMA
                     DRGFRVLALAYKMLPLPRESGLPLSEEECETDLIFAGLAGLADPPRAEVPAALKELRS
                     AGIRVLMLTGDHSATARTVARHVGIDHGGVITGRELEPMSDEDLLSTVGRISVYARIA
                     PEHKLRIVRALQSGGEVVAVTGDGVNDAPALREAAIGVAMGQTGTDVAKEAAGMVLAD
                     DNFATIGMAVREGRKLFENLRKAVRYYLAAKVALVGSSLIAVLLHLPVPFTPIQIIVT
                     ELFMDLGASVAFTTERAEGDVMARPPRDPKHPFMDSAMQAGIFAGGISLCAAVIAAYL
                     WVFSQGAESAHAQSLAFVTWMMGHVLLALHMRSERQPLLSLGPLSNPAMVIWGVGALA
                     VALLAANLPILRDALKVTPLAPQEWAFALIALLLTTSWWEVWKLARWERR"
BASE COUNT         1126 a         1988 c         1951 g         1299 t
ORIGIN      
        1 cgactattac cccgatctga gaagaattgg agccaagctt ggcaatccta gcggcgacat
       61 cggtggtgct ggcttggacg gcctgcatac ctttaacgct cttcccgacc gaatcggcgc
      121 cggaagcggc agcggcccgc gcctgttggg tggctaagga gacttcctcc gaggcaccgg
      181 ccactcgtgc gatagacaga ttcagttgtg cgaccgaggc ggaggtcttt tcgatcgatc
      241 tcgcctgttc ctgagagccc gtggcgattt gatcgatcgc tcgagacagc tggaccatgt
      301 tggcggacgt gatctctgtg gcttccgact gctcctgaga tcccctggcc ataccctgaa
      361 tggtggactc ggtctcctga agcgccgcac cagtctggga tgcagtggtg ccgagttcgg
      421 ttgaactgca ggccagtgtc tctacagaag actggacccg gcccaccagg tctcgcaggc
      481 tcgcgttcat gcgtctgaag gcgagtccga ggacgtcgga ctcggagcgg gccacgacat
      541 cacgggtcag gtcgcccgag gcaacggcct gagcagtctc ggcgagtgct cgctcggagg
      601 cagcgaggtc tttgaatgcg agggcggcgc gcccgatctc gtcaccagtg ctgaccttga
      661 tctcgtagtc gaggtctccc gaagcaatgt gtcgtgcgcc ttcggccaga tgtcgggcag
      721 tcgaggccaa tgatcgtgcg aacagcacgg cgaagaaggt agccagaagg agagccacga
      781 gggctgctat cgccgagccc tggaggaagg agttggctgg tgcttcaacg acctccagtg
      841 gcagggcctc tacgtatgac caggtggtct gcgtgagcgg cacgaccata gcatcgaagg
      901 gtttgccctg cagtttccag ttgaagcggg cggacttctt ggcgcctatg gcaccggaga
      961 gatcggtttg gcccagcggg tcaggacttg cattgttgcc ccaccgcttc tcctggacca
     1021 gcggctgaag gatctccggc ttcagcggaa tgaccggcct catgacccaa ccggcatcca
     1081 catcgctggc tactaccagc ccactctcgt ccagcagcac tccggtcgac tgctttccca
     1141 tcctgctttt ggcggcttcg atcgctcgct gcagcggtgc tagtccggct cttgaacgca
     1201 ggacggcaat tacccgaccg tccacgccct ttatcggtgt cgaatggtag atactcgccg
     1261 cattcgtaac agtggagatg gagacgccgc tgatgaaggc tttcccattc atcgctgcct
     1321 tgaaatagtc ccgttggggg gagggagcgg gaagcgtcga tgcggtatcg gcgacgaaat
     1381 tgccggccga gtccatgatc ccgacggctt cgaggccctc gtcggaggcg gagtcgagca
     1441 ggctcttgag ggccccctgt atctgcttca tctgggtggc tcgtgcaaca gggtcggccg
     1501 cggcaaattc ctggatgacc gggtttgcgc taagtgattg aaggtccctg atccgctgga
     1561 tgttccagcc gtcaacggtg ctcgccaccg tcatcgcatc agacgcgagt gccgattcgg
     1621 cctgcgttct gaggccctcg gaagcctggc ggtatcctat cgccgtaagc gcgatggcga
     1681 gtgccgcgca gaggccaaca cagaggatcg tgatcttgat tccgacagaa aggtcaccga
     1741 tcctggagtg ccccctacca tgctttgggg ctgtctgcat cttcgccttc tcccattctg
     1801 ggttgccaca ccacagtctt tgtcactctc gtcatgctgg ttgatcgaat tccgatgtca
     1861 acctcgtgcc accgtcgggg tcggcccaag acctactcgg accgattgtt cccattggag
     1921 gatgtccata acatccccaa gctgctggag cagtgcgaag cgaaggacag gctcctggtt
     1981 accacgtgat agggggacct gccttgcgtt ggcaggtccc cctgtcgtcg gaagctatgg
     2041 ccggctgagt gtgccggcat tccctccctg gcgctcaggg atcgaacgcc aatcgtcggt
     2101 cctccgcttt tgaaccacta cttcagacgg tctggcaaga cccgcttggc cgccgtctga
     2161 ctcatccagt ttgaacctgg ccaccatctt gcgcagctct tccgccgtgc gggagagttg
     2221 tgcggcctgg gcactcatct cctcgacctg ggcactcatc tcctcggacg atgcggagac
     2281 ttcctcggtt gccgccgagt tctcctccgc cgtcgcggcg gaggactcga tagcctggga
     2341 gacggttcga gccagcaact ccatctgttc agtcgcggca agggactgct cggtgaccgc
     2401 gctgatcgtc tccagggcag cggtcacccc cttggaggag gcagccatct gctggcttgc
     2461 cgctgcgatc tggcttacct gctgtacagt ctcctccacg ctgcggacga tctgttcgag
     2521 cgcttcgccg gcacgtgcgg ccaggtcgga acccgcctct gcctcacttg ccccactgtg
     2581 catcgcatcg accgccgcct ttgtgcctgc ctgaacgctg cgaaccagtt cggcaatctg
     2641 cctagtctcc tttgacgacc tctccgccag cttgcgtacc tcgtctgcaa cgactgcgaa
     2701 gccgcgcccg tgctccccag cccttgccgc ctcgatcgcc gcgttgagcg ccagcagatt
     2761 ggtctgctcc gcgatgtcgt cgatggtctc aaccactgcc ccgatctgct cggacgcgcg
     2821 gccgagttcg tcgatctgag tggccgtgcg atcgacggtc tctttgagcc tgctgatgcc
     2881 ggcaatggtc tcctggacca tctggttacc cctgacggcg gcatccctgg tcttctcgct
     2941 ctcgtgggcg acctggttgg cgttggcggc cacctgctcc acgccaacag ccatctgtcc
     3001 ggcagtgtcg ttggcggact gaatgccccg agcctgatcg ttggacccag acgcgacctg
     3061 tgagattgcc tgggtgagat cgctcatggc gctggtggtt tcctgggcta cggtggcctg
     3121 ctgctgcgcg ccctgcgcga cgctctggat ggtaacgtta acctgctgga cggcgtcgcc
     3181 ggtctggttg gcggcctcgc cgagctgggt gctggttgcg gcgagctcgc gggcagagga
     3241 ctgggtctca gcaaccatct ctctcaggtg cgaagacatc tcctggaacg caatgcccag
     3301 gacatcgtga tcgcttctca gctcgacctg agccgttaga tcgccatctg cgatcctgcg
     3361 ggccacatct gccatgccct taaagccatt cgccatcctc ttgaaggact tggccagcaa
     3421 tccaagctcg tcccgacgcc gcagcattcg ctctaccgtg cctgcgtcca gatcctgccc
     3481 gaggccgcct tccgcgtatt cgatggctgc cgcggcaagg aaggtaatgg gcgaagagaa
     3541 cgagcgagcg atccagatag cgaggggaat gactatgacg gcgaccaacg ccaggatgct
     3601 ccaactgatg cggttgatct cgtcgacggc cccgagcgcc gcgtcgcgac cctgaactgt
     3661 ggcaaccgac catccgttga cgcccaccgg cccgtatccg gctaccttgc cggctccatt
     3721 gtagctgtag tttgcagtgc cagtctgtcc ttgaaccatc gccttcgcca tggccgccat
     3781 ggcgggatcc gtcgccttgg tggggttatc cttcatgatg ttgtcggcca ctggatgcgc
     3841 gatggtggtc ccgtccttgc tcagcacgaa ggtgtagctg tccttcatgg agcctttcgc
     3901 caggttctgc tcgtagaagc ccttcagcga gacggtgccg aggataatac caccggtctt
     3961 gccgtccttt gtcttcactg gagacacggc aatgacgacg atgtctccag tggccttgga
     4021 caccagcgca tcggagatta ccggattccc tgacattgca ggcttgtagt aatccctatc
     4081 ggtgacgctg gcaccttctt ggtcctccgt gttggcgatg atggtgccgc tcccgttcac
     4141 cagggagaag gtcgcgaagg tgccaccgaa ctgcttctgg gcagtcttta gaaaaggtct
     4201 ggctatcgca ggatccatgc tcctgacact atcggtttct gccagaacct ggacctcctt
     4261 caaccgtgtg tttgcccaat cctgagtcct ctgagcattc agctgcgccg tctcctgcaa
     4321 cttctccgac gcctcggtct ccagcgctga gccggactgc cggccggcca cgaggcctac
     4381 cactaaagca ggcagtatgg cgaggagcag cacgatgatg aagaatttgc ctttgaacga
     4441 ctgcagaaaa cccaacttct cgccatcacg tccccttgtc tttgacgcca ttgtctcctc
     4501 cttttcgaag tcgatctatc gtttatcccg ctttcggggt agctcgctca gcatgggttg
     4561 aggacggcag tggccgcagc ggaaacgacg gggatttcgc tgaagagggt cacaggtacg
     4621 aggagggctg tggccggcca ccgcgtcaag ggctatgtac cggggatttc tggtcgaact
     4681 accacctaca gcaaaagtgc tactgctata gatcggcagc tacatgagat tctttagaca
     4741 atgggaaata ttggggatta atggccctct gaagggggac attggtccca ataggcgagt
     4801 cggtggagtg atggtacaga ggcaatagtt gaggcatcgg gtctggtgcc aagagactcg
     4861 ccggcgctga aagcagtaat gctccgccac agtagaggtg acacgtgctg tcatgctgag
     4921 cgaagcgaag catctcctcg tgaacggaga gatccttcgc tgtcgctcag gatgacacct
     4981 gtcaggatga ctgtggtcga gaagttgttg gggaggcggc gaagtagcag tctcttaccg
     5041 ccgctcccag cgcgccaact tccacacctc ccaccaactc gtggtgagga gaagggcgat
     5101 cagcgcaaag gcccattcct gtggggccag cggggtcacc ttcagtgcgt ccctgaggat
     5161 cggcaggttc gcagccagca gcgctaccgc caaagcgccc acgccccaga tcaccatcgc
     5221 cgggttcgag agggggccga gggaaaggag tggctgtcgc tccgagcgca tgtgcagcgc
     5281 gagcagcacg tgacccatca tccaggtgac gaaggcgaga ctctgcgcat gagccgattc
     5341 tgctccctga gagaagaccc acaggtaggc ggcgatcacc gccgcacaga gtgagatccc
     5401 accggcgaag atgcccgcct gcatggccga gtccatgaag gggtgcttcg ggtcacgggg
     5461 aggccgagcc atcacgtcgc cctccgcccg ctccgtcgtg aaagccacgg aggcccccag
     5521 gtccatgaat agctcggtga cgattatctg gataggggtg aagggcaccg gcaggtggag
     5581 caggacggcg atcagggagc tgccgaccag ggcaaccttg gcggccaggt agtaccggac
     5641 ggcttttcgc aggttctcga acagcttgcg accctcgcga actgccatcc cgatggtggc
     5701 aaagttgtcg tctgcgagca ccatgcccgc ggcctccttc gccacgtcgg taccggtctg
     5761 ccccatggca accccgatgg ccgcctctct gagggccgga gcgtcgttta cgccgtcccc
     5821 ggtgacggcc accacctcgc cgccggattg cagagccctg actatccgca gcttatgttc
     5881 gggggcgatg cgggcataca ccgagatacg gccgactgta gacagcagat cctcgtcgct
     5941 catcggctcc agttcacgcc ccgtgatcac ccctccatga tcgatgccga cgtgacgggc
     6001 aacggtccgg gcggtggcag agtggtcgcc cgtgagcatc aacaccctta ttccggcgga
     6061 gcgcagctcc ttgagggcgg caggcacttc ggctctgggg ggatcggcaa gcccggccag
     6121 gccggcaaag atcagatccg tttcgcactc ctcctccgac agcgggaggc cgctttcccg
     6181 aggcaacggg agcatcttgt aggctagtgc gagcaccctg aatccccgat ccgccatggc
     6241 ctcggctgcc gagaatgcgg tggtccgagg ctgctccccg aaaggagact ccagttcctc
     6301 cactacctgg cggctacagc gagcgatgac tgcttcggga gcccctttga cggcaaccag
     6361 gagt
//
LOCUS       DATALS010000563         1978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_85513,
            whole genome shotgun sequence.
ACCESSION   DATALS010000563 DATALS010000000
VERSION     DATALS010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85513"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..589
                     /locus_tag="VHS06_12565"
     CDS             275..589
                     /locus_tag="VHS06_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988985.1"
                     /translation="MSEEKKTEGVKVHKTGKKSWEHKYDLSDMQAVYRRGHWNSWDEI
                     IGWLKAHGEGDNELTPGETAAIVEDLQGVRDARVPFTNDPRKAYDLVFEHGVGTRKKK
                     AA"
     gene            868..>1978
                     /locus_tag="VHS06_12570"
     CDS             868..>1978
                     /locus_tag="VHS06_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX2988986.1"
                     /translation="MNPKPFALPEGFRFGVATAGFQVEGGYNGPREPRNNWYWWEKAG
                     RVEPSGIALDFWNRYEAQLDLAKDVGCDSFRLSVEWARCEPSKGKIDKAAFKRYSAIL
                     KATHGQDKEPIVTHHQITHPEWLGMDFWLEPESPALFAAWVRMAVQHLGDYCRRWVTV
                     NEPNTYIINSYLAGIFPPGRLFDRRAALRAVDNMLTGHVKAYEEIHRQQPTATVATNN
                     FCFSIYQFDRLLLDLLLARRNGVLKGDLSEWLHARKLAWEAAHSQPSLGERLLRRIVS
                     TAFPLGKALPNAADAVYDSPYRCTLDVTQFDYYNPIASNHVRLPGHRSAKGRHWVPAR
                     LLWDDPPTPEQFLSYMHLGHEPGLQLWITENGLCNP"
BASE COUNT          444 a          613 c          583 g          338 t
ORIGIN      
        1 cgaccaccac ttcgtggtgg tcgccacgtc ccccaagaag gtccgtgtgc tgaatcaaga
       61 ggccggcagc gccgggctca gcacgatggc aaccaacagc ctcggctgga acggcgattg
      121 catcaaggag gccgggcaag agtagcctac gacggtccga gccatcgatg gccttcccta
      181 taggtgtcga ggccggcctg agagactggt agccatctgg gtcaatatcg gcggagtctc
      241 tcctcagccc gccatcaatt cataggagga agaagtgtct gaagagaaga agaccgaagg
      301 tgtaaaggta cacaagacag gcaagaagag ttgggagcat aagtacgacc tctccgacat
      361 gcaggccgtc taccggcgag gacactggaa cagctgggac gagatcatcg gctggctgaa
      421 agcccacggc gagggcgaca acgagttgac ccccggcgag accgccgcca tcgtggagga
      481 cctgcaaggc gtgcgcgacg cacgtgtccc attcacgaat gatccccgaa aggcgtacga
      541 cctggtgttc gaacacgggg tcggaacccg gaagaagaag gctgcttgag tatcgattga
      601 ccctcacggc gaccaccact tcgtggtggt cgccacgtcc cccaagaagg tccgtgtgct
      661 gaatcaagag gccggcagcg ccgggctcag cacgatggca accaacagcc tcggctggaa
      721 cggcgattgc atcaagtcct tcatcaccaa acctggttga cccttcggcg tccccgcatc
      781 cctatccaag caacactgag acccggcact ttcagcgcga gccaacaggc aacatacctg
      841 gcacgtggat aaagagtgtg gcagaacatg aatccaaagc cattcgccct gcccgagggg
      901 ttccgtttcg gcgtcgcaac tgccgggttc caggtcgagg gcggctacaa tgggccaaga
      961 gagcccagga ataattggta ctggtgggaa aaggccggaa gggtggaacc gtccggaatc
     1021 gccctcgatt tctggaaccg ctacgaagcg caattggacc tggccaagga tgtcggttgc
     1081 gactcctttc gcctgtcggt ggagtgggca cgatgtgagc cttccaaggg gaagatcgac
     1141 aaggctgctt tcaagcgcta ctccgccatc ctcaaggcca ctcacggcca agacaaggag
     1201 ccgatcgtta cccaccacca aatcactcac cctgaatggc tggggatgga cttctggctg
     1261 gaacccgagt cgcctgccct cttcgctgcc tgggtacgaa tggccgtgca gcatctcggc
     1321 gactactgcc gccgctgggt aaccgtaaac gagcccaaca cctacataat caacagctac
     1381 ctcgcgggga tctttccgcc cgggcgtctc ttcgatcggc gagccgccct ccgtgcggtg
     1441 gacaacatgc tgacgggaca tgtgaaggct tacgaggaga tccacagaca gcaacccaca
     1501 gcgacggtcg ccaccaacaa cttttgcttc tccatatacc agttcgaccg gctattgtta
     1561 gatcttctcc tggcacgccg caacggggta cttaagggcg acctttcgga gtggctccat
     1621 gcgcgaaagc tggcatggga ggcggcccat tcgcagccat cgctgggtga acggctgctg
     1681 aggcggatag tgtccacggc cttcccgctg gggaaggcac tgcccaatgc cgcagacgcg
     1741 gtctacgaca gcccgtaccg atgcaccctg gacgtaaccc agttcgacta ctacaacccc
     1801 atcgcgtcca accacgttcg actccctggg cacaggtcgg caaagggccg ccactgggtt
     1861 ccggccaggc tactgtggga cgacccaccc accccggaac agttcctctc ctatatgcac
     1921 ttaggtcacg agcctggact tcagttgtgg atcaccgaga acgggctgtg caaccccg
//
LOCUS       DATALS010000564         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_85861,
            whole genome shotgun sequence.
ACCESSION   DATALS010000564 DATALS010000000
VERSION     DATALS010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85861"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1917)
                     /locus_tag="VHS06_12575"
     CDS             complement(<1..>1917)
                     /locus_tag="VHS06_12575"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988987.1"
                     /translation="ESRHVRVYGFAELSQGSRSNLTTSFDPASGAIMAIDRDHPDWVR
                     TIGFDRHPTAYESSKQRSLAYEPYEKRPLSNHLEDRDDVMAVEQLDLDIAADGQEKLC
                     WVLSFSDKGIDVARASLERWRDWEKGLAKLVEAYAQALSVSHIMVPEKVITHGVLWAK
                     ANMLRVMGRYQTGAGFTNDPGAASNVVARDVAWFVYGCDYLRPDYAADLLRLAARFQE
                     PRGKIVEYWDARTGQSYDYGLNVNDDTPLFILAAGHHYLVTLDRQFLQDIYPAVARAA
                     RYLLSQKDYRGLIFCSARGTDVYGICGWRNVIPDVGISGAVTEVNSEVYASLRMAAEM
                     ARALGHNGDVGEFLDEAVGLADAINRHLLNPGNGLYYLNIDPDGNAIADVTCDEIFPV
                     MFGVSPPEVAFRIISRLSRPDFWTLAGLRTASRNSPEYTPDRELGLRGGVWPGVSFWY
                     AFAAARYHPESMVRALASFQHYNEDPIRYNTVPGQFSEWFDGESLANRGMRLSPWEPP
                     RLLWAAVEGMLGVVHSIDGCLVQPLVPGHWKWVAVRHLPHAQREETMFLARQGGKLHI
                     YGNAHGLSSDLPFTRYEQDVSDGTRCLHPDASVVALMRKDDLLVCLGNSADRTITVPL
                     SLEGVLSRDRSYYL"
BASE COUNT          353 a          637 c          583 g          344 t
ORIGIN      
        1 ccaggtagta cgacctgtcc cgcgatagca ccccctccag gctcagcgga acggtgatag
       61 tcctgtccgc cgagtttcca aggcacacca gcaggtcatc cttccgcatc aaggcgacga
      121 cgctggcgtc ggggtgcaga cagcgggtcc catcgctgac atcctgctcg tagcgagtga
      181 aggggaggtc tgaggagagc ccgtgggcat tcccgtagat gtggagctta cccccctgcc
      241 tggcaagaaa catcgtctcc tcgcgctggg cgtggggaag gtggcgcacc gccacccatt
      301 tccagtgccc ggggaccagc ggctggacaa ggcagccatc tatactgtgc accaccccca
      361 gcatcccctc gaccgctgcc cacagcaaac ggggcggctc ccagggcgac agcctcatcc
      421 cccggttggc aagcgactcg ccgtcgaacc actcagagaa ctggcccggc accgtgttgt
      481 accggatcgg gtcctcgttg taatgctgga aggatgcaag cgcccgcacc atggattcgg
      541 ggtggtagcg agccgcggcg aaggcgtacc agaaggagac gcccggccat acacctcctc
      601 gcagtccgag ctcccggtcg ggtgtgtact cggggctgtt gcgcgaggca gtgcgcaatc
      661 ctgccagggt ccaaaagtcg gggcggctca gccggctgat gatgcggaag gcgacctccg
      721 gcgggctcac gccgaacatc acgggaaaga tctcgtcgca ggtgacatcg gcgatggcgt
      781 ttccatcagg gtcgatgttg aggtagtaga gtccgttgcc ggggttcagg aggtgccggt
      841 tgatggcatc agccagtcct actgcctcgt cgaggaactc tccaacatcc ccgttgtgac
      901 ccagcgccct cgccatctcc gcggccatac gcagggatgc gtagacctcg gagttcacct
      961 ctgtgacggc cccgctgatc cccacgtccg ggatcacatt tcgccatcca cagatgccgt
     1021 acacgtccgt tccacgggct gagcagaaaa tcagccctcg gtagtccttc tggctcagca
     1081 agtacctggc ggcgcgggcc acagcaggat agatgtcctg aaggaactgt cggtccagcg
     1141 ttaccagata atggtggccg gcggccagaa tgaagagcgg cgtgtcgtcg ttgacgttaa
     1201 gcccgtagtc gtaggactgg ccggtacggg cgtcccagta ctccactatc ttccctctgg
     1261 gttcctggaa gcgggccgcc agccgcaaca agtcggccgc ataatccggc cgcaggtagt
     1321 cacagccgta gacgaaccac gcaacgtcgc gagcaaccac attgctggcg gctcctgggt
     1381 cgttggtgaa ccccgccccc gtctggtatc gccccatcac ccgcagcatg ttggccttgg
     1441 cccagaggac gccgtgggta atcaccttct ccgggaccat gatgtgagaa acggaaaggg
     1501 cctgagcgta cgcctccacc agcttcgcca gccccttctc ccagtcgcgc caccgctcca
     1561 acgacgccct ggccacatct atccccttgt ccgagaagct gagtacccag cacagcttct
     1621 cctgtccatc tgccgcgata tccagatcca actgctccac cgccataacg tcgtcgcggt
     1681 cctccaggtg gttgctcagc ggccgcttct cgtaaggctc gtaggccaga ctgcgctgct
     1741 tggacgactc ataggcggtg gggtggcggt cgaacccgat agtccttacc cagtccggat
     1801 ggtcgcggtc gatggccatt atggcacctg aggcgggatc gaaggaggtc gtcaggttgc
     1861 tccggctgcc ctggctgagc tccgcaaagc cgtacaccct cacatgccgg ctctcgc
//
LOCUS       DATALS010000565         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_86309,
            whole genome shotgun sequence.
ACCESSION   DATALS010000565 DATALS010000000
VERSION     DATALS010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86309"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VHS06_12580"
     CDS             complement(<1..125)
                     /locus_tag="VHS06_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX2988988.1"
                     /translation="MSPTYIALLRGINVGGKNKLPMSELVQLFVEEGCSNVRSYI"
     gene            complement(127..780)
                     /locus_tag="VHS06_12585"
     CDS             complement(127..780)
                     /locus_tag="VHS06_12585"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2988989.1"
                     /translation="MSSGRLTPNVRVALVGVCAFLVIALMVQFRLLSGLDDAVVQARK
                     GVAVPILDGWSWLAAILISIELSMLYASAGALALRRAGMGRWSLAPFGFLLTAPLEIA
                     MKLTLRQPVVPLQFRRSGGVYPFFSVELGGSFPSGHALRAGFLCLFLAILLWERGGTI
                     NRSASVALVVLALMLGFARVYMGHHWMTDVVAGLLLGGSLALLLAPPVARRLKVRPL"
     gene            complement(910..1716)
                     /gene="fdhD"
                     /locus_tag="VHS06_12590"
     CDS             complement(910..1716)
                     /gene="fdhD"
                     /locus_tag="VHS06_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX2988990.1"
                     /translation="MQDTLSTERSDGTTVYTWTEQGGWGSAQGHFGEEMALALYLDRV
                     ELATLMCTPEKLNFLITGFLRSEGFIGSLDDIALMRVCDEENVADVRLMRPKKQPTGK
                     RILTSGCGGGVTFDSGDGVVAVTSDLRVAPAQLLAHMKAMLLPSDNRHGERGGLHVSA
                     LADEKQLLVVARDVGRHNTLDKIWGECLFREIDTRGKVLLTTGRLSSEMMIKAAKMGV
                     PITVSLNSPTSRAVQLARELGITVVGYARGGRLSVYAHPERLGVTDPAAR"
     gene            complement(1929..>2661)
                     /locus_tag="VHS06_12595"
     CDS             complement(1929..>2661)
                     /locus_tag="VHS06_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988991.1"
                     /translation="GVWPGVAFWFARAAAPYYPEATVTALSCYQHYNENPLHNNTVPG
                     QFSEWFDGESLANRGMRLSPWEPPRMLWAAITGMLGIQHPSESCTLHPNVPDRWKWLA
                     VRRMPHADGEEALFLARQNDGQLHLFHGPLHGLATDLPHTGYERDVTQSIESLHPDAH
                     VVALAKADEVLICIGSSGLETATMPLEIHDLLQENTQYRIVLYDSEFKGWITGASRPG
                     KDLRRMASSVEPGGFRLFRLTRVRP"
BASE COUNT          552 a          890 c          767 g          452 t
ORIGIN      
        1 tggatgtagc tgcggacgtt gctgcatccc tcctcgacga aaagctgcac caactcgctc
       61 atcggaagct tgttcttacc gccgacgttg atgcctcgaa gtagcgcaat gtaggtaggt
      121 gacattctag agcggcctca ccttcaaccg tcgggccaca ggaggcgcca acagcagcgc
      181 cagcgatccc cccaacagca aaccggcgac cacgtcggtc atccagtggt gccccatgta
      241 gaccctcgcg aaaccaagca tcaacgccag caccaccagc gcaacagaag ccgagcgatt
      301 gatagtgccg cccctttccc acagcaggat cgccaggaag aggcagagga aaccggcacg
      361 caacgcgtgg ccactgggaa aggagccgcc cagctcgacg ctgaaaaagg gatacactcc
      421 cccggaacgg cggaactgca gcggcacgac cggctgacgc agcgtcagct tcattgcgat
      481 ctccagcggg gcagtcagca ggaagccgaa gggagcgagt gaccatcgcc ccatgcctgc
      541 tcgccggagg gccagagccc ccgccgacgc gtacagcatc gacagctcta tggagatcag
      601 gatcgcggca agccaggacc acccgtctag aatgggaacc gccaccccct tcctcgcctg
      661 gaccacggcg tcgtccagac cggagaggag gcggaattgc accatcaagg caatcaccag
      721 gaaggcgcac actcccacca gcgcgaccct cacgttcggt gtaagccggc cactggacaa
      781 agcaccccgc ctacgctcag atttccccat cgatccgccg tctttcgaac tctgttggca
      841 gactcatccc atcaacggga aggccgaggg agagggagag ggcagacgcc gaaccgaaag
      901 cgacgaggat caacgagcag caggatcggt gacgcctaac ctctcggggt gcgcatagac
      961 ggagagccgc ccacccctgg cgtatccgac aacggtaatt cccagctccc tcgccaactg
     1021 aaccgctcta ctggtgggag agttgagaga gaccgtgatg ggcacgccca tcttggcagc
     1081 cttgatcatc atttcggatg aaagccggcc cgtcgtcaac agcacttttc ccctggtgtc
     1141 gatctctcgg aagaggcact ccccccatat cttgtccagg gtgttgtgcc ggccgacgtc
     1201 tcgggcgacc accaacagct gcttctcatc ggcgagggcg gaaacgtgca gaccgcccct
     1261 ctccccgtgg cgattatcgg aaggcagtag cattgccttc atgtgggcca gcagttgagc
     1321 gggagcgact ctcaaatccg aggtcaccgc aaccacacca tcgccgctat cgaaggtcac
     1381 tccccctccg cagccggagg tcaggatccg tttgccggtg ggctgcttct tcggtcgcat
     1441 cagccgcacg tcggccacgt tctcctcgtc gcagacccgc atcagcgcga tgtcgtccag
     1501 gcttccgata aacccttcag atcgaaggaa gccggttatc agaaagttca gcttctctgg
     1561 ggtgcacatc agggtggcca gctccacccg atccaggtag agcgccagcg ccatctcctc
     1621 cccgaaatgt ccctgcgcac ttccccatcc gccctgctcg gtccatgtgt acaccgtcgt
     1681 accgtccgac cgctcggtcg agagagtgtc ctgcatagcc actcagacct tccaccaaca
     1741 gacttgttag tgcagtcaat tatacccgtt ggcttgtcgc cagccaatcg cccgacaggc
     1801 tgacaagaac gaattccgaa cagagctatt ctcttcgctg cggcgactca ccccctcaag
     1861 actcgaagga ttccatgatt ctcctcgtgg gcgcctgcaa tgggcggtac ggtccaaccc
     1921 tgccgatatc agggtcgcac gcgggtcaac cggaacaggc ggaacccgcc gggctccacg
     1981 gaactggcca tccgcctcag atccttccca gggcgagatg cccccgtgat ccagcccttg
     2041 aactcgctgt cgtacaggac gatccggtac tgggtgttct cctgcaacag gtcgtggatc
     2101 tccagaggca tcgtggccgt ctcgagaccg gaagaaccga tgcagatcag cacctcgtcc
     2161 gccttggcca gcgctaccac gtgagcatcc ggatggaggc tctcgatgga ctgggtgaca
     2221 tcgcgttcgt agcccgtgtg aggaagatcg gtggccagac catgaagcgg cccgtggaac
     2281 agatgtagct ggccatcatt ctggcgtgcc aggaagagcg cctcctcccc atcggcatgg
     2341 ggcatcctgc gcacggccag ccacttccat cggtcgggaa cgttcgggtg aagggtgcaa
     2401 ctctcgctgg ggtgctgtat tcccagcatt cccgttattg cagcccagag catccgcggc
     2461 ggctcccagg gggagaggcg catgccccgg ttcgccagcg attccccgtc gaaccactcg
     2521 gagaactgcc cgggcaccgt gttattgtgt agcgggttct cgttgtagtg ctgatagcag
     2581 gacagggcag tcaccgtggc ctcggggtag tagggcgccg ccgccctggc aaaccagaag
     2641 gccacgccgg gccacacccc a
//
LOCUS       DATALS010000566         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_86448,
            whole genome shotgun sequence.
ACCESSION   DATALS010000566 DATALS010000000
VERSION     DATALS010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86448"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VHS06_12600"
     CDS             complement(<1..829)
                     /locus_tag="VHS06_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX2988992.1"
                     /translation="MLGHVADIPLFNAAPNTLSWLSINLLDSASRWAVPVYVMLSGAL
                     LLDPARDESPTQFYRKRLGRLGIPIVFWSAFFMLFAIFYTHASTPREALRNLANGEPY
                     LHLHFIFRLAGLYALTPALRVLVKHASRTLLIQTVLILFVIWSADSLINGLRYIEPSA
                     FARFAPFMAYYLCGYLLRDLVPSRRTLLLALVGLVASILALVAWSSLMARYYDVSPFP
                     SRPMLLHDFLSPVRITMGLCSWILLTWLFRRPLPTGSLLARISAWLAPLTLGIYLVHP
                     "
     gene            complement(783..1514)
                     /locus_tag="VHS06_12605"
     CDS             complement(783..1514)
                     /locus_tag="VHS06_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988993.1"
                     /translation="MYQRVQPSLVAVQYTWESELGRRDVIGAGVVVGEDGTVMTSLAL
                     VDQRIPDDQMKDFKILLPTDSPEVVEEVDAQFLGRDERGDVAFVKAKPGKEARAWQAV
                     RFVDRAAKVGEMVWSVGLLPKMAGYKAAAASAYTPPTSKQGARPAHMPGLRRHPAQGE
                     LAPPFEGGIGRVRHTVARQCCRYHLTASTVQFGRAHQPAARAPGAPTPGPPATHGVRR
                     SPSRARDPGGRARPRRRHPALQCRP"
BASE COUNT          380 a          584 c          648 g          281 t
ORIGIN      
        1 aggggtgtac gaggtagatg cccagggtga gcggcgcgag ccaggcactg atgcgtgcca
       61 gaagcgagcc ggttggcagg ggacggcgga agagccacgt cagcaggatc cacgagcaga
      121 ggcccatggt gatgcgcacg ggcgagagaa agtcgtgcag gagcatcggc cgggacggga
      181 aaggcgagac gtcgtagtag cgagccatga gggaggacca ggcgaccagg gccaggatag
      241 acgccacgag accgaccagc gcgagcagga gcgtgcgtcg agaggggacc agatcgcgca
      301 ggagataacc gcagaggtag taggccatga acggggcgaa acgggcaaag gcgctgggtt
      361 cgatgtagcg caggccgttg atcagtgaat cggccgacca gatcacgaag aggatcagca
      421 cggtctggat cagcagcgtg cgcgaggcgt gtttgaccag gacgcgcagg gcaggcgtga
      481 gggcgtagag gccggcgagg cggaagatga aatgaaggtg gagatagggt tcgccattgg
      541 cgaggttgcg cagtgcttcg cgcggcgtgg aggcgtgggt gtagaagatg gcgaagagca
      601 tgaagaatgc cgaccagaag acgatgggga ttccaaggcg gccgaggcgt ttgcggtaga
      661 actgcgtggg cgattcgtcc cgcgcgggat cgagcagcag ggctcctgaa agcatgacat
      721 agaccggaac ggcccagcgg gaggcggaat ccagaaggtt gatggagagc caactgaggg
      781 tgttaggggc ggcattgaag agcgggatgt cggcgacgtg gccgagcacg accgccaggg
      841 tcccgagcac gcgaagggga tcgccgtact ccatgcgttg cgggcggtcc aggggtgggc
      901 gctccgggtg cgcgggcggc gggctggtgc gcacggccga actggacggt gctggcggtg
      961 agatggtacc gacaacactg gcgagcgaca gtgtgtctga cacggcctat gcccccctcg
     1021 aaaggcggcg ctagctcccc ctgtgcaggg tgccgacgaa ggccgggcat gtgtgccggg
     1081 cgtgcaccct gcttcgatgt tggaggagta taggcagagg cagcggccgc cttatacccc
     1141 gccatcttcg gtagcagccc cacggaccac accatctccc ctaccttcgc ggcccgatcc
     1201 acaaaccgca ccgcctgcca ggcccgcgct tccttccccg gcttcgcctt cacaaacgcc
     1261 acgtccccac gctcatcacg tcccaggaac tgcgcatcga cctcctccac aacttccggg
     1321 ctgtcggtcg gcaacaggat cttgaagtcc ttcatctggt catcgggaat ccgctggtcc
     1381 accagcgcca gcgaggtcat caccgttcca tcctcgccga ccaccacgcc cgccccgatc
     1441 acatcgcgac ggcccagctc gctttcccac gtatactgca ccgccaccag cgacggctgc
     1501 acccgctgat acaacgcagg cacgttcagc cgggctggtc cttgctgagt agatgcagcg
     1561 gccggcgtcg gctgagcttg cccccctggt cccgcttgcg tctggctcca ggcaacctga
     1621 ggcaacacgg taagcaacgc gattgcagca aatcgattaa ccatagcgtt tcggatcctt
     1681 tactcacgat cctagcgtca aatccccgcc gcggcgaacc cccggcaggc acaacgctca
     1741 gttcctcccc gcatcccggt acagattgct cgttccaggc ggccgctgcc tctgcctata
     1801 ctcctccaac atcgaagcag ggtgcacgcc cggcacacat gcccggcctt cgtcggcacc
     1861 ctgcacaggg ggagctagcg ccgcctttcg agg
//
LOCUS       DATALS010000567         6862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_87765,
            whole genome shotgun sequence.
ACCESSION   DATALS010000567 DATALS010000000
VERSION     DATALS010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87765"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1259
                     /locus_tag="VHS06_12610"
     CDS             168..1259
                     /locus_tag="VHS06_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEX2988994.1"
                     /translation="MPLIARAPMRISLAGGGTDLEDYYARYGGMVVSAAIDKYFYAVV
                     SESGSQRLQLISANYGSFHSQEAVEDLVWDGNLALPKAVVDHFGLPAGHDVFMASEIP
                     PGTGLGSSSAAAVCLSLALASKQGKQLSRAQLAEMASLLEIERLGMPIGKQDQYASAF
                     GGINAITFSRDGTRVEPLTLPEDRIRELEKHLMLFFMGSSRSSSSILRQQRESTRRGD
                     RRVLESLHAIKKMAVQMRRALDDGDLAEVGGLLDLSWSRKKTLASTVTNGEIDESYRI
                     AIEAGALGGKVTGAGGGGFLLLFCPPGRQSEVSAQLEARGLHRIGFQFDFLGAHILMG
                     DSEATGSEPLRRAGFWVPDQPDEGDPRAA"
     gene            1362..2783
                     /locus_tag="VHS06_12615"
     CDS             1362..2783
                     /locus_tag="VHS06_12615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2988995.1"
                     /translation="MEKYDLLVIGGGECGLAAAKQGATLGARVAMAEPGAVGGTCLSR
                     GCIPKKALVRSAYVYHLVKGAELFGIRSDVPSVNWSRVMERKDRLLRQLELEKCDSLD
                     ALSVPILRGKAKFVSPHELLVGEQTVLADRVVLAVGSRAAKVPISGAELAMTSDHALA
                     LRELPESVVIVGGGVIAMEFAHIWNRFGVRVSVVEMGERILGTMDAQVATELARLSAD
                     RGIEILTNARLEQIRRADGGYSVRVQLLEKTRDIDCKAVLLAAGRVPNVDGLGLEAAG
                     VGLERGRVVVDRYLQTSVGSIYCGGDAIGGYCLAPVAAYEGRLAALNALRGNTEMADY
                     STVAYTVFSLPPVSSVGLTESQARDAVGRVGVGILPMASVPRAVVEGETDGFIKVVTD
                     AVTGRILGAHIVGSRSEELIHIFSVAMKGMLTREQLADVIPVHPSFAEGAVEATKSAG
                     KVLDGEERCRAVRRPQFGDITGR"
     gene            complement(2770..3135)
                     /locus_tag="VHS06_12620"
     CDS             complement(2770..3135)
                     /locus_tag="VHS06_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2988996.1"
                     /translation="MAPAGEGDGVPATPGAVVARGAAVAAGAEVAPGAEVAPGAIVAP
                     GVAPTGLVAAGADVAETGLVAAGAAGADVGAGAPEGAQAAASPIVAAAKAHPMTRRRL
                     TDTAVGSFVTCSRTLSSVL"
     gene            3314..5131
                     /locus_tag="VHS06_12625"
     CDS             3314..5131
                     /locus_tag="VHS06_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HEX2988997.1"
                     /translation="MIDIDGLLPRVSKPARYAGGELNAVHKDWDSVSTRVALIYPDVY
                     EVGMSNLGLQILYDLVNREPTLLAERAYAPWVDMEASMRERGIPLFSLESRRPLADFD
                     LLGLSLSLELTYTNALNVLDLAGLPVLAEEREDHFPIVIAGGSGSYNPEPMAPFFDLF
                     AVGDGEEVLLELMRLHSRMKADLAGAHDTRVPRTEFLREAAKLEGVYVPSLYRLDDCG
                     FPIPATDGVPGTIKARRISKLGPSPVKPVVPFVEAVHDRAMVEVQRGCTRGCRFCQAG
                     MVYRPVRERSPREVLENADELLSNTGYDELSLVSLSTADYSGIKEVTNTLGAQYPERR
                     IKVSLPSLRVDSFSVELARALKESYGGGLTFAPEAGSQRLRDVINKGVTQEDIEGAVE
                     AAFGRGWNSVKLYFMIGLPTETDEDLDEIAKLAYRIRDIGRRHAGSRTKVKVSVGALI
                     PKPHAPFQWSGQMPYTTIREKMVHLQRGIRGPGLAFSWHETETSVLEAALSRGDRRTS
                     RVILRAWQAGCRFDAWAEQFDFGRWRDAFAAEGMDIHGAAERVFSLEERLPWDHISIG
                     VKKEFLAEEYRRAMRAELSPDCRSGRCLVCGIRQSYGGC"
     gene            5131..5778
                     /locus_tag="VHS06_12630"
     CDS             5131..5778
                     /locus_tag="VHS06_12630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEX2988998.1"
                     /translation="MGQRLRVTFSRGEAMKYLSHLELMRMWERVFRRAGWHLAYSQGF
                     NPHPRFSFAVPLPVGVAGEAELLELYLDEARPLDEAAEQLRGQLPKGAGVVRVDEVPD
                     TAPSMQRQLVGAEYVATCPPLSSVEALRGETARMMGASALPRQRIKEGKARSYDLRPM
                     IHSLEVQCADERPAVVKMDLRTDAAGAGRPDEVLRELGIEPAECHITRTRLIFRD"
     gene            5793..6512
                     /locus_tag="VHS06_12635"
     CDS             5793..6512
                     /locus_tag="VHS06_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEX2988999.1"
                     /translation="MEIEGVVNRAHLLRHLADLIAAVDQPCPVRVAIDGVDAAGKTRL
                     ADELVMPLEDRSRVVIRASIDSFYRPRAERYRRGPDSPEGYFYDSFDYVALRELLLDP
                     LGPGGDRQFRVASFDTELDEAIDDRLRHAPVDSVLLFDGVFLMRPELEDCWDFRVFVD
                     VDFEEAIRRAVQRDQPLFDTEDSTLERYWTRYVAAQRLYIETARPLDQADAIVVNQDP
                     AHPRLIVPYPPQAESLTSGEE"
     gene            complement(6683..>6862)
                     /locus_tag="VHS06_12640"
     CDS             complement(6683..>6862)
                     /locus_tag="VHS06_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2989000.1"
                     /translation="TDRHIQPSLGYINADKVGFLIHRSLLQMAHPCMMRALMPWQLFG
                     LMMGGRGDQRYVSVF"
BASE COUNT         1234 a         1952 c         2346 g         1330 t
ORIGIN      
        1 ccagggtgat cctattcgtg ttgtcccagc cggaggactg tgccgtgcag gaggtgatcg
       61 tcacacctct taccgaaacc tcgtggccgt agttgagacg cggagatgcg gagacacggg
      121 gacacggaga cgtggggacc ggggggcggc agtagggagg gactggggtg ccactaatag
      181 ccagagcgcc catgcgcata agcttggcgg gtgggggaac cgacctggag gactactacg
      241 ccaggtacgg tgggatggtc gtgagcgccg ccatagacaa gtatttctat gcggtggtct
      301 cggagagcgg ctcgcagcga ctgcagttga tctcagcaaa ctatggaagc tttcactccc
      361 aagaggcggt ggaggatctg gtttgggatg gtaaccttgc cctccccaag gcggtggtgg
      421 accattttgg cctccccgcc ggacatgacg tcttcatggc atcggagata ccccctggaa
      481 cgggactcgg atcgtccagc gcggcggccg tctgcctctc cctcgccctt gcctccaaac
      541 aggggaagca gctgtcgcgg gcgcaattgg cagaaatggc ctcactctta gagatagagc
      601 gactcgggat gccgatcggc aagcaggatc agtacgcctc tgccttcggg ggcatcaacg
      661 ccatcacgtt cagccgcgat ggaacgcgag ttgagccgct gacgttgccg gaagaccgca
      721 ttcgggagct agagaagcat ctgatgctct tcttcatggg gagttcgcgt agctcttcca
      781 gcatcctcag acagcagcgg gagtcaaccc gacgcgggga ccgccgagtg ttggagtcgc
      841 ttcacgctat caagaagatg gccgtgcaga tgcgaagggc gttggatgac ggcgacctag
      901 cagaagtggg cggactgctg gacttgagtt ggagccggaa gaagaccctg gccagcacgg
      961 tgaccaatgg ggagatcgac gagtcgtatc gcattgccat cgaggccggg gctctcggtg
     1021 gcaaggtgac tggagccggc ggcggcggct ttctgctgct cttctgtcct cctgggcgcc
     1081 aatcggaggt gtctgcccaa ctcgaggcgc gtggactgca tagaattggc ttccagtttg
     1141 acttcctggg tgcccacatc cttatgggcg acagcgaggc gacagggagc gagccgttgc
     1201 gtcgtgccgg attctgggtg ccggatcaac ccgacgaggg cgaccccaga gccgcgtagt
     1261 gttcgagaca cggacatggg gtatcctagc tgaacgttgg tgaggcatga gccgcctgtt
     1321 cgcggttcat ctccggatag ggagctgtcg gaggacgtag ggtggagaag tacgacctac
     1381 tggtaattgg tggcggtgag tgcggactcg ccgcggcgaa gcagggcgcc acgttaggtg
     1441 ccagggtggc catggcggag ccgggagcgg taggcggtac atgcttgagc aggggatgta
     1501 tccccaagaa ggccctggtt aggtccgcct acgtctacca tttggtcaag ggagccgagc
     1561 tcttcggaat acggtccgat gtcccttcgg taaactggag ccgtgtcatg gagagaaagg
     1621 accgactcct tcgccaattg gagttggaga agtgcgattc cctggatgcc ctctctgttc
     1681 ccattctgcg tggcaaggcg aagttcgtct cccctcacga gttgctggtc ggcgagcaaa
     1741 ccgttctggc cgatcgcgtc gtcctggcgg ttggctccag ggccgccaag gttccgatct
     1801 cgggggcgga gttggcaatg accagcgacc acgcgctggc tcttcgggag ttgcctgagt
     1861 ccgtggtgat agtgggcggc ggcgtcatcg ccatggagtt tgcccatatc tggaaccgct
     1921 tcggtgtgcg tgtgtccgtc gtggagatgg gcgaacggat actgggcacg atggacgccc
     1981 aggtggcgac tgaactggcg cggctttcgg cggacagggg tatcgagatc cttacgaacg
     2041 cccgactgga gcagattcgc cgagccgacg gcgggtattc tgtcagggtg caactgctgg
     2101 agaagacccg cgacatcgac tgcaaagcgg tactgctcgc cgcggggagg gtccctaacg
     2161 tcgatggcct tggcctcgag gccgcgggcg tgggactcga gcgaggcagg gtggtcgttg
     2221 accggtatct gcagacttca gtgggcagca tctactgtgg aggagatgcc atcggtgggt
     2281 actgtctcgc cccggtcgcg gcatatgagg ggcgccttgc cgcccttaat gccctgcggg
     2341 gcaatactga aatggctgac tacagcaccg ttgcctacac cgtgttttcc ctgcccccgg
     2401 tatccagtgt cgggttgact gagagccagg cccgcgatgc tgtaggccga gtaggtgttg
     2461 ggatcctccc aatggcctca gttcctcggg cggtggtgga aggagagact gacggtttca
     2521 tcaaggtggt caccgatgcg gtgacgggtc gaatactggg agcgcacatc gtcgggtcgc
     2581 gctcggagga attgatacac atcttttcgg tggcgatgaa gggcatgttg acgcgcgaac
     2641 agcttgcgga cgtgatcccc gtgcatccca gctttgccga aggggccgtc gaggctacga
     2701 aaagtgctgg caaggttctt gatggtgagg agagatgtcg agcggttcgg cgaccccaat
     2761 ttggggacat cacaggacgc tagaaagggt tcttgaacac gtgacaaagg atccaacggc
     2821 agtatcggtc aatctacgtc gagtcattgg gtgggcctta gcagcggcca cgatcggact
     2881 ggcagcagcc tgcgcgcctt cgggggcacc ggcacccaca tcggctccag cggcacccgc
     2941 ggccactaaa cccgtctcgg ccacgtcggc gcctgcggct actaggcccg tcggggccac
     3001 gccgggggcg actatggcac caggtgcaac ctcggcgccg ggagcgactt cagcgccggc
     3061 agccacggcg gcaccgcgag ctaccaccgc gcctggagtc gcaggcacgc cgtcgccttc
     3121 cccagccgga gccatgacgc ccacacctac gagggtgccg tagctactta cggttgcggt
     3181 gcaccaaacc acagggcggg cgggtcgtcc tcgacccgcc cgtcggccaa ctttccagac
     3241 tgtctccgcc ggccttgtcg cggcagaatg gtataatttc cttctccatt cgtgctaggg
     3301 aaggtcgctg ctgttgatcg atatcgatgg tctcctgccc agagtctcta agccggcgcg
     3361 ctacgccggc ggtgagctga acgcggtgca caaggattgg gactcggtct ccacccgtgt
     3421 agcactgatc tatccagacg tatacgaagt cggcatgtcc aatctcggtc ttcagatcct
     3481 gtacgatctg gtgaaccggg agcccactct tctggccgag cgcgcctacg ctccttgggt
     3541 ggacatggaa gcgtcgatgc gtgagcgcgg cattccgctt ttctcactgg aatctcgacg
     3601 cccgctggcc gacttcgacc tgctggggct gtctctctcc cttgagctta cctataccaa
     3661 cgccctgaac gtgctggatc tggcagggtt gccggtgctg gccgaggaga gggaggatca
     3721 cttccccatc gtcatcgccg gtggtagcgg gagctacaac cctgagccga tggccccctt
     3781 cttcgacctg tttgccgtgg gcgacgggga ggaggtgcta ctggagttga tgcgcctcca
     3841 cagccggatg aaggccgatc tagccggcgc gcacgacacc cgggttccga ggacagagtt
     3901 ccttcgcgaa gcagcgaagt tggagggcgt ctacgtcccc tccctctaca gactcgacga
     3961 ttgcgggttt cccattcctg ctacggatgg ggttcccggc acgatcaagg cacggcggat
     4021 ttccaagttg gggccgtcgc cggtgaagcc ggtcgtgccc ttcgtggagg cggttcacga
     4081 tcgggcgatg gtggaggtgc agcggggctg cactcgaggc tgtcgcttct gccaggcggg
     4141 aatggtctac aggccggtta gggagcggtc tccccgagag gtgctggaga acgccgatga
     4201 actgctctcc aataccgggt acgacgagtt gtcgctggtc tcgctcagta ccgccgatta
     4261 cagcggtatc aaggaggtca ccaacacgct gggtgcccag tatccggagc ggcgcatcaa
     4321 ggttagcctt ccatcgttac gtgtcgattc cttctcggtc gagttggctc gcgcgctgaa
     4381 ggagtcttat ggcggcgggc tcactttcgc gccggaggcc ggcagccagc gattgcggga
     4441 cgtgatcaac aaaggggtga cccaggagga catcgagggc gccgtggagg cggccttcgg
     4501 gcggggttgg aattcggtca agctgtactt catgatcgga ctccccaccg agaccgacga
     4561 ggacctggac gagattgcca agctggcgta caggatcaga gatatcggca gacggcatgc
     4621 ggggagcagg acgaaggtca aggtgtcggt gggagccctc attccgaagc cccacgcccc
     4681 cttccagtgg tctggacaga tgccatacac tacaatcagg gagaagatgg tgcacctgca
     4741 gcggggcatc cgcgggcccg gcctcgcctt cagttggcac gagactgaga ccagcgtgct
     4801 cgaggcggcg ctctccaggg gggatcgtcg caccagtcga gtcatccttc gagcctggca
     4861 ggccgggtgc cggttcgatg catgggctga gcagttcgac ttcggtcgat ggcgcgacgc
     4921 tttcgctgcc gaagggatgg atatccatgg tgcggccgag cgggtgttct cccttgaaga
     4981 gaggctcccc tgggatcaca tctccatcgg ggtcaagaag gagttcctgg ccgaggaata
     5041 ccggcgggcc atgcgagcgg agctatcacc agattgcaga agcgggcgtt gccttgtctg
     5101 cggcatccgc cagagctatg gaggttgctg atggggcagc ggttacgggt taccttctcc
     5161 aggggagagg cgatgaaata cctctcccac ctggagttga tgaggatgtg ggaacgggtc
     5221 ttcagacggg caggctggca tctggcctac agtcagggct tcaatcccca tccacggttc
     5281 tcctttgcgg tcccgctacc ggtcggtgtc gccggggagg cagagcttct ggagttgtac
     5341 ctggacgagg cgcgtccgct ggacgaagcg gcggaacagc ttcggggaca actgccgaag
     5401 ggcgccggtg tcgtgagggt ggacgaggtt cccgacacgg cgccatcgat gcagcggcag
     5461 ttggtgggag ccgagtatgt cgcgacctgc ccgcctttgt catccgtgga agcgctgagg
     5521 ggcgaaaccg cgcgtatgat gggggcgtcg gcgctccctc gccagcgcat caaggagggc
     5581 aaggcgcgca gttacgactt gcgtcctatg atccactcgt tggaggtgca gtgtgccgac
     5641 gagcggccgg ctgtggtcaa gatggatctg cggaccgacg ccgccggggc gggcaggccc
     5701 gacgaagtgc tgagggaact tggaatcgaa cccgcggagt gccacatcac tcgtacgcgt
     5761 ctcatattca gggactgaca tgggggtgac ccgtggaaat cgaaggagtg gtcaaccgag
     5821 cgcacctgct tcgccatctg gcagacctga tcgcggccgt ggaccagccc tgcccagtga
     5881 gggttgcgat cgacggggtg gacgctgccg gcaagacccg gctcgcggat gaattggtca
     5941 tgccgcttga ggaccggagc cgcgtagtca tccgtgcgtc catcgatagc ttctatcgtc
     6001 ccagggcgga gcgctaccgg cgcggcccag attccccgga gggctatttc tacgacagct
     6061 ttgactacgt cgctcttaga gaattgctgt tggatccgct gggccccggc ggcgaccggc
     6121 agttccgcgt ggcttccttc gacactgagt tggatgaggc gattgatgat cggctccgcc
     6181 atgctcccgt ggattcggtg ctactgttcg atggtgtgtt cctgatgcga cccgagttgg
     6241 aggactgttg ggatttcagg gtgttcgtgg atgtggactt cgaggaggcg atcagacgag
     6301 ctgtgcagcg agaccagccc ctgttcgaca cggaggattc tactctcgaa cggtactgga
     6361 ctcgctacgt ggcggcccag cggttgtaca tcgagactgc aagaccgttg gatcaggccg
     6421 atgccatcgt cgtgaaccag gaccctgccc atccgcggct gatcgtcccg tatccgccgc
     6481 aggcggagag tctgaccagc ggcgaggagt gatcctccgc ccatgttaga catgtagggc
     6541 agggccctgt gccctgccgc ccggtggccg tgttccatgc gattgcggcg gggcagagcg
     6601 ccccgccctt gtatcttgag aatgcgttag tataggccga caagttcacg tcggatgcgg
     6661 cagtccgtcc ccgcccttgg tcttagaaga ccgatacgta gcgttggtcg ccgcgtcctc
     6721 ccatcatgag cccgaacagt tgccagggca taagagcccg catcatgcaa ggatgggcca
     6781 tctggagcag ggatcgatga atcaagaacc ccactttgtc ggcattgatg tatccaagac
     6841 tcggctggat gtggcggtcc gt
//
LOCUS       DATALS010000568         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_88571,
            whole genome shotgun sequence.
ACCESSION   DATALS010000568 DATALS010000000
VERSION     DATALS010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88571"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VHS06_12645"
     CDS             <1..698
                     /locus_tag="VHS06_12645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2989001.1"
                     /translation="ENPVEARYLWLASALQTIGDVPEAAFVQELATKNGAQQFQVRIS
                     RIFAPKGEERGAAAILESITDQQLPNARRDSGHPELSPSYLVDALTGLHNRRGFSALA
                     TQLMKLADQMKRHLAFLVCSVEGVAQAGNANATQTDDQVLLAVAAILRDTFRASDIIA
                     RVGKEEFAVLAVGSFNGGAEELAGRLQSAVEKQRSLGKFDHQLLISMGLIPYDPLYPR
                     DIDDLLTRIPGDV"
     gene            complement(695..2086)
                     /locus_tag="VHS06_12650"
     CDS             complement(695..2086)
                     /locus_tag="VHS06_12650"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX2989002.1"
                     /translation="MIGTICRRIASTELPLLILVIPVLLFPRGPFPLAGLAVILGIWL
                     ARWIGFRRITLATPLDLPMLLLLAMSLQALYPSTDLALSLPKLYGIVVGVAVYYAVVN
                     DVRDFRDWWIGSYALLMAAVGVAAVGLVGTSWITDKLPAGAEIYARLPRLIHSVQSSS
                     GEISGIQPNELGGTLAFLLPVPFALLLWGRGIRWVVGAATLCLAAPVLVLTQSRSSLL
                     AAAVVVVLLLGLRWWRIGYLMLATAVAGIAAGGLVGREVLYRWIIQADYVGDATTKLA
                     NRQEIWGRTLYMIQDFPFTGVGLNSFAKVLDGLYPSAFIGSVAEIPHAHNIYLQTAVD
                     LGLAGLMALLGVCLTTLWIAFRVYRRTDGPTRGAIAGLGAGLLAYMLFGMTDAITLGA
                     KPLPLLWGIIGLIVAADRLNACGESETGEQGVRTMDGAAARTAVALYWAIAVLFALMA
                     YLVVAMAIVGVVA"
     gene            complement(2083..>2596)
                     /locus_tag="VHS06_12655"
     CDS             complement(2083..>2596)
                     /locus_tag="VHS06_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2989003.1"
                     /translation="EIERGIALDPGNSKGYEAAGDLYRVEKDFERADNRYAQAVQKNP
                     GDVWIAVRRVDNLLDAGRNETAVGLLKEATERFSGEPHLYYLLGRAYSHLGDSESALL
                     AAKTAVSMAERTAAVNLSRGVGYGLALAEIYEGAGMQREAEAAYRSVLDLDPKNNDAT
                     KGLRRLGERS"
BASE COUNT          588 a          853 c          721 g          434 t
ORIGIN      
        1 ccgagaaccc tgtggaagca cgctatctgt ggttggcgag tgcgttgcag accattgggg
       61 atgtgccaga ggcagccttc gtccaggaac tggcaacgaa gaatggagcg cagcagttcc
      121 aagtcaggat ctcccgaata ttcgccccaa aaggcgagga gcgaggcgcc gcggcgatcc
      181 tcgagtcgat cacagatcag caattgccga acgcgcgaag ggactcaggc catcctgagc
      241 tgtcgccctc ctacctggtc gatgctctca ccggcctcca caaccgccgg gggttctccg
      301 cgttggccac gcaactcatg aagctggcag accagatgaa gcgacacctc gccttcctgg
      361 tgtgctccgt ggagggagta gcccaagccg gcaacgcgaa cgcgacgcaa accgatgatc
      421 aggtgctcct ggcagttgca gccatcctgc gcgacacctt ccgcgcttca gacataatcg
      481 cgcgggtggg caaagaggaa tttgcggtgt tggccgtcgg atcctttaat ggtggcgccg
      541 aggaactggc cggacggctc cagtcggcgg tcgaaaagca gagatccctc ggcaagtttg
      601 accaccaact actgatcagc atgggactga taccgtacga tcccctttac ccgagggata
      661 tcgacgacct cttgacgaga attccgggag acgtctaggc cacgacgccc acgatagcca
      721 tcgccacgac caggtaggcc atgagcgcga agaggaccgc aatcgcccag tagagcgcca
      781 ccgcggtcct agccgctgcg ccatccattg ttcgaacgcc ctgctcccca gtttccgact
      841 cgccacaggc gttcagtcga tccgccgcga cgatcagtcc gatgatcccc cacaacaacg
      901 gcaaaggctt cgcccccagg gtgatggcat cggtcatccc gaagagcata taggccagta
      961 accctgcacc aaggcccgca atcgcgcccc tggttggccc atcggttcga cggtagactc
     1021 tgaaggcaat ccacaatgtc gtaagacata cccccagcag tgccatcaga cccgccaggc
     1081 ccaggtcaac cgccgtctgc aaatagatgt tgtgcgcatg agggatttcg gcgacagacc
     1141 ctatgaaggc agacgggtaa agaccgtcca gcaccttcgc gaagctgttc aaccccactc
     1201 ccgtaaaggg aaaatcctgg atcatataga gggtcctgcc ccagatctcc tggcggtttg
     1261 ccagcttcgt cgtcgcgtca ccgacgtagt ctgcctgaat gatccaacga tagagcacct
     1321 cccggcccac gagtccacct gccgctatcc ctgcaactgc ggtagccagc atcaggtagc
     1381 cgatgcgcca ccaacgcagt ccaagcagga ggaccacgac gacggcagca gcaagcagac
     1441 tgctccgaga ctgagtcaac acgagcacag gggcggccag acaaagggtg gcagcaccaa
     1501 caacccaacg tatcccccgc ccccacagca gtaacgcgaa cggaactggc agcagaaacg
     1561 ccagggtccc gcccaactcg ttgggctgaa tgccggagat ctcccccgaa gagctctgga
     1621 ccgagtggat gagccgaggt agccgagcgt agatttccgc acccgcgggc aacttgtcgg
     1681 tgatccagct cgtgcccacc aggcctacag cagcaacccc cacagccgcc atcagcaagg
     1741 catatgatcc tatccaccag tcccgaaaat ctctgacgtc gttcacaacc gcatagtaga
     1801 ccgccacccc aactacaatc ccatacagct tggggaggct gagcgccagg tcggttgacg
     1861 gatagagggc ctgcaggctc atcgccagca gcagcagcat gggaagatcg aggggcgttg
     1921 ccagagtaat acgacggaag ccgatccacc tggcaagcca gatcccaagg atgacggcaa
     1981 gacccgcgag cgggaagggg ccacggggaa agagcagcac ggggatgaca aggatcagca
     2041 gaggcagttc cgtactcgca atacgacggc agatcgttcc aatcaactgc gctcacccaa
     2101 ccggcgcagg cccttggtgg catcgttgtt cttgggatcc agatcaagaa ccgaccggta
     2161 ggcagcttca gcttccctct gcataccggc gccttcgtat atttccgcaa gagccaatcc
     2221 gtagccaacc ccgcgggaga gattcaccgc cgcggtccgc tccgccatcg acaccgccgt
     2281 ctttgctgcc aacagcgcgc tctcagaatc accaagatgg ctgtacgccc tgccgagcag
     2341 ataatacagg tgaggttctc cggagaaccg ctcagttgcc tccttcaaca gaccgacggc
     2401 agtttcgttt cgacctgcat ctagcaagtt gtctacccgc ctgactgcga tccacacgtc
     2461 gcccgggttc ttctgaaccg cctgcgcata ccgattgtcg gccctttcaa agtccttttc
     2521 caccctgtag agatccccgg cggcctcgta ccccttcgag ttgccggggt ccagggcaat
     2581 gcctcgctct atctcg
//
LOCUS       DATALS010000569         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_89443,
            whole genome shotgun sequence.
ACCESSION   DATALS010000569 DATALS010000000
VERSION     DATALS010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89443"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..227)
                     /locus_tag="VHS06_12660"
                     /pseudo
     CDS             complement(72..227)
                     /locus_tag="VHS06_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
     gene            973..2133
                     /locus_tag="VHS06_12665"
     CDS             973..2133
                     /locus_tag="VHS06_12665"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX2989004.1"
                     /translation="MDLSRSYGWEMPTKVIFGPGSSRQAGEQLRELGGKKALLVTDRG
                     VEAAGVLAGIYEGLSAAGIAYVLYNGVEPNPTIKNVREAAALFQAEGCDCLLGVGGGS
                     SMDTAKAAGAVLNNPDVNIRDMEGTGHVPNAIPPMLAIPTTCGTGSEVTSAAVITDSE
                     RKYKIPILSPLLYPKVALVDGSLLTKLPTAVIASTGMDALCHAIESYTNLNTNPISDA
                     LDLQAISMISKWIRPAVANGNLEAMSHMVLAATIAGMAFPNTRLTIVHSMSQPVGGHY
                     GVPHGVANAVLLPLVMEFNLMGNAERFADVARAMGEDTTGLTTMEAARLSVKAVREMS
                     REIGIPENFKSYGVKEESIPALVEDSMKSGNIPVNPVKVTREALAAILRKSM"
     gene            complement(2187..2537)
                     /locus_tag="VHS06_12670"
     CDS             complement(2187..2537)
                     /locus_tag="VHS06_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX2989005.1"
                     /translation="MGAGAPTLADILDNLEKPGLDPRDSLPKPILRKDVLKMEDLKEG
                     MLLQGTVRNVVDFGAFVDIGVKHDGLVHVSEMADRFVRNPLDVVGVGDVVTVRVLGVD
                     HQRGRIQLSMKNPG"
     gene            complement(2476..>3334)
                     /locus_tag="VHS06_12675"
     CDS             complement(2476..>3334)
                     /locus_tag="VHS06_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX2989006.1"
                     /translation="EGTTIYPHVPMKRWTEAKEKIVALARRHGVNVVAVGNGTASRET
                     EALVAEVISEGNEDLAYVMVSEAGASVYSASEVARQEFPELEAAQRGNISIARRLQDP
                     LAELVKIEPKAVGVGLYQHDVDQKELAKELDRVVVSCVNFAGVDLNTASVSLLQYVSG
                     INRRVAENLVAYRNEHGPFTDRTEIKKVAGVGAATFVQAAGFLKVPGGANPLDDSFIH
                     PESYDLCRKLMEKLPPRQRWRAAAAAGRPFPEGDGAAGGHTGIAGRGVGGRSSHPGGY
                     PGQPGKAGT"
BASE COUNT          609 a         1101 c         1058 g          566 t
ORIGIN      
        1 cgccaggtgg ccgagcaggt gaagcccgcg attgtgcaga tcaccacttc gcaggagtcc
       61 atcggctctt tcaaccagcc atccccgccg accagctggc cgtcgaaggt cctgccgtcc
      121 ggcagcgcca ccatcagctt ggtggctccg tcgacgacat ggttattggt aaggatgtag
      181 cccgccttgt catagatgac gccggagccg acaccggtct gctcttgcgg gaatggctgg
      241 ttgaaagagc cgatggactc ctgcgaagtg gtgatctgca caatcgcggg cttcacctgc
      301 tcggccacct ggcggacggc ggtcgcgaac tccgtctgcg attgagcaga ggccactgcg
      361 gcggtcaacg ggctcgaact tgcgtgctgg cctgttgccg tggacagcaa acctgaggat
      421 ccagccccaa accagacggc agcgcagatg gccacggtcg ccaggccccg ccccagcaca
      481 ccaggtttca atagagattt catcttcgag ttcctccgta gcttcaccct gctccactaa
      541 gcgactttcc cttgttgaca tgcactttcc cttcaaagaa aggttgactg catccaggga
      601 aagctgctta gctcgcgcag gcgggatccc tttccggcaa accccatcgc cgggtcgttc
      661 aacgatttct gtatgagttt tagcaccacc gggccgcgca ttggctaaga ctatattggg
      721 aaaacgtaaa tagtgtgtaa aacgtgacgc ggagacacgg agacgcgggg acacggagag
      781 ggagttcctt ggggcgctac cagcttgccg atgtagcccc cccccggccc ttcctgggag
      841 cgcgggcgtc tcgcccgcaa gaccgggatg cggcttgagc gcgcacccca catcatgtaa
      901 gctgcagagg caggttgaag attggcccgt ggtggtccag tacacccgaa ccgcgggcca
      961 ggagggtgaa agatggatct gagtcgcagc tacggctggg agatgccaac caaggtgatc
     1021 ttcggccccg gctcgagtcg gcaggcagga gagcagctga gggaactggg gggcaagaag
     1081 gcgctgctgg tcacggaccg aggtgtggag gccgccggtg tactggcagg catctacgag
     1141 gggctgagcg ccgccggcat cgcctacgtc ctgtacaacg gagtggagcc gaaccccacg
     1201 ataaagaacg tcagggaggc cgcagccctc ttccaggcgg aagggtgcga ttgtctgctg
     1261 ggcgtgggtg gcggttcatc catggacacg gccaaggccg cgggtgcagt gctgaacaac
     1321 cctgacgtga acatccgcga catggagggg acggggcacg tcccgaacgc tatcccgccc
     1381 atgctcgcta ttcccaccac ctgcggcacc ggctccgaag tgaccagcgc cgcggtgatc
     1441 accgactcgg agcggaagta caagatcccg atcctctcgc cgctgctcta cccaaaggtg
     1501 gcgctggtgg acggcagcct gctgacgaag ctgccgacgg cagtgatcgc gtccacaggc
     1561 atggacgcgc tgtgccacgc catcgagtcc tacaccaacc tgaacaccaa cccgatcagc
     1621 gacgcgctgg acctgcaggc gatctccatg atctccaagt ggattaggcc ggcagtggcc
     1681 aacggcaacc tggaggcgat gtcccacatg gtgctggccg ccaccatcgc cggcatggcc
     1741 ttccccaaca cccgcctcac catcgtccac tcgatgagcc aaccggtggg cggccactac
     1801 ggggttcccc acggggtggc caacgcggtg ctgctgccgc tggtgatgga gttcaacctg
     1861 atgggcaacg ccgagcgctt cgccgacgtc gcccgcgcca tgggcgagga cacgacgggg
     1921 ttgacgacca tggaggccgc gcggctctca gtaaaggccg tgagggagat gtccagggag
     1981 atcgggatcc cggagaactt caagtcctac ggggtgaagg aggagtcgat ccccgccctg
     2041 gtggaggact ccatgaagag cggcaacatc ccggtaaacc cggtgaaagt gacccgcgag
     2101 gcgctggccg cgatcctgcg caagtcgatg tagcgaggat gtggagtgcg gcgcatgagc
     2161 gacgcactcc acactcggct cctgcgtcag ccggggttct tcatgctcag ctggatgcgg
     2221 ccgcgctggt gatccacacc cagcacccgc accgtaacca cgtcgccgac gcccaccaca
     2281 tccagcgggt tccgtacgaa gcggtcggcc atctccgaga cgtggaccag cccatcgtgc
     2341 ttcacaccga tatccacgaa ggcgccgaag tccaccacgt tgcgcacagt gccctggagc
     2401 agcatcccct ccttcagatc ctccatcttc agcacgtcct tccggaggat cggcttgggc
     2461 agcgagtcgc gcgggtcaag tcccggcttt tccaggttgt ccaggatatc cgccagggtg
     2521 ggagctccgg cccccaactc cgcggccagc gattccagtg tgaccccctg ccgctccatc
     2581 tccttccgga aacgggcgac ccgctgcggc agccgctcgc catctttggc gggggggcag
     2641 cttctccatc agcttgcggc agaggtcgta gctctccgga tggatgaagc tgtcgtccag
     2701 ggggttggca ccgccgggga ccttcaggaa gccggcagcc tggacgaagg ttgccgcccc
     2761 gacgcccgcc accttcttga tctcggtccg gtcggtgaac gggccgtgct cgttccggta
     2821 ggccaccagg ttctcggcaa cccgccggtt gattcccgag acgtactgca gcagcgacac
     2881 cgaggcggtg ttcaggtcga cgcctgcgaa attgacgcag gagaccacca cccgatccag
     2941 ctccttggcc agctccttct gatccacgtc gtgctggtag agccccacgc ccaccgcctt
     3001 cggctcgatc ttgaccagct cggccagggg gtcctggagc cggcgggcga tggagatgtt
     3061 gccgcgctgg gcagcctcca gctcagggaa ctcctgccgg gcgacctcgg aggcggagta
     3121 gacggaggct cccgcctcgc tgaccatcac gtaggccaga tcctcgttgc cctcggagat
     3181 cacctcggcc accaacgcct cggtctcccg ggaggccgtg ccgttgccga cggcgaccac
     3241 gttcaccccg tgcctgcggg ccagggcgac gatcttctcc ttcgcctcgg tccaccgctt
     3301 catcggcacg tgcgggtaga tggtcgtccc ctcg
//
LOCUS       DATALS010000570         7992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_89631,
            whole genome shotgun sequence.
ACCESSION   DATALS010000570 DATALS010000000
VERSION     DATALS010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89631"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1828)
                     /locus_tag="VHS06_12680"
     CDS             complement(149..1828)
                     /locus_tag="VHS06_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2989007.1"
                     /translation="MSTPRRIVVVGGVAAGASAATKARRTDEGAEIVIFERGPYVSFA
                     NCGLPYYVGGEIPSLDDLLIMTPERFADRFRIDVELHQEVTGILREEHEVEVLNLLTG
                     ERRRVPYDRLILAPGGRPVLPPIPGIDRPGVFSLTTVPEAESLRRWIDFRGAKQAVVI
                     GGGFIGIEAVEALLSLGLEVHLVEKLPQMLPQMDPEMAGLLDAHLSERGVRMHLGAEV
                     VGFDGEERLEGVRLASGERLPAQVAVVAIGVRPELTLARDAGLEVGESGGVVVDDHMR
                     TSDADVFACGDVAEVVNRITGRRVRLPLAGPANKEGRVAGANAAGGDSRFPGVIGTSI
                     VKVCDLSAGKTGLGEREAKAAGFDPLVSFLHPLDHAGYYPGAQKMTFKLVADRTSGRV
                     LGAQVVGPQGVDKRIDVIATAIHGNMTVEDLEGLDLAYAPPYSSAKDPVVMAGFVTAN
                     QWRGEVEAVTPADVAGKIANGAPLRLLDVRTEEEYQEGHIPGAINVPLDELRSSLDKI
                     RRDGPITVYCAAGYRSYNACKILGYHGHRVVNLSGGFTSWSQTNPGIVEKS"
     gene            complement(2099..2171)
                     /locus_tag="VHS06_12685"
     tRNA            complement(2099..2171)
                     /locus_tag="VHS06_12685"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2136..2138),aa:Thr,seq:tgt)
     gene            complement(2290..2688)
                     /locus_tag="VHS06_12690"
     CDS             complement(2290..2688)
                     /locus_tag="VHS06_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2989008.1"
                     /translation="MRLADLVADLMNVANPRGWRSPYDFESPDLSPRERLVRLEYAAL
                     QAWRQSCYQADRDELEYALQECRALLALDSLEGEVAVQARVNQRTIRIVRVVKHHEGQ
                     LTRMNRHFWWVPFSIGMVMMISCCLHCIGG"
     gene            complement(2864..3955)
                     /locus_tag="VHS06_12695"
     CDS             complement(2864..3955)
                     /locus_tag="VHS06_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX2989009.1"
                     /translation="MAAHRVTLIPGDGTGPEIVEATRRVLEATGTQFDWDVQEAGVDV
                     LEKYGTPLPDQVIESIKKNKVALKGPITTPIGKGFRSVNVALRKSLGLYACLRPCKSY
                     KGVRSRYQNVDLVVVRENMEDLYAGVEFQKGSEGAKRLIALAAETGAGQIPEDSGISI
                     KSISVTGTRRVVKWAFEYARANKRRKVTAVHKANILKFSDGLFLQVASEVAKEYPDIE
                     FDDRIVDNMCMQLVQKPELYDVLVLPNLFGDIISDLAAGLVGGLGVAPGGNFGEGNVA
                     IFEPTHGSAPKYAGLNKVNPSATILSGMLMLRHLGEFAAADRLERAFASVVAEGRNVT
                     YDMKPSPDDPTAVGTSQFADAIISRMKEQ"
     gene            complement(4177..4734)
                     /locus_tag="VHS06_12700"
     CDS             complement(4177..4734)
                     /locus_tag="VHS06_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HEX2989010.1"
                     /translation="MTDSIRLTLPLKREDVKKLRAGDLVLLSGTMLTGRDAAHKRLFE
                     LIQQGKELPVSIADQCIFYTGPTPAAPGRPIGSAGPTSSYRMDKYAPLLMDHGLLGMV
                     GKGLRSREVKDAMVRNGCVYFGATGGSGALLSLCIKAAEVVAYPDLGTEAVRKITVED
                     FPAIVIVDSTGKDLYESGPAKYAKA"
     gene            complement(4727..5593)
                     /locus_tag="VHS06_12705"
     CDS             complement(4727..5593)
                     /locus_tag="VHS06_12705"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX2989011.1"
                     /translation="MPSVASSEITAKVRDLCIAAAYELPRDVVDSFRRALDREESPQG
                     KAIFKELLRNAEVAVAEHIPYCQDTGVAAIFVRKGPRVTIEGGTLTGAITEGVRRGYT
                     EGYLRASVVTEPLFERKNSGDNTPPVIHIEESDKDELVISIVPKGAGSENMSRLVMLT
                     PSSGWEGIKKEVVDTVRRAGPNPCPPIVVGVGIGGNFEKCAYLAKRSLLRNLGEPNPD
                     PRIAALEAELLEEVNKTGIGPAGLGGTVTALAVHIETYPTHIACLPLAINLGCHAYRH
                     KTAVLLGGENHD"
     gene            complement(5752..6672)
                     /locus_tag="VHS06_12710"
     CDS             complement(5752..6672)
                     /locus_tag="VHS06_12710"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEX2989012.1"
                     /translation="MKISMIGAGRVGSTTLYTLLLEGGVTEMAIVDVDKPRAEGEALD
                     LRHALALTQNCKIVAGDYDVTEGSDMVIITAGIPRKPGDTRLDLLKKNTELMGGILEG
                     VLKHNPDTFILMVSNPVDVLTYQALKTSGFPSNKVFGLGTVLDTTRLRSLIGEWLGVS
                     PDQVVAYMMGEHGDSMVPVVSGATVAGIPLKDYPGYNDQKLEELITAARFGGAEVIKR
                     KGGTFYSVAPSIVSVVRAWRNDQKKVLPISSLIDGHFGDLAGMTISLPCIVGRNGREA
                     ILDPHFSAKEDAEFRNSFKVLKEAATSVGL"
     gene            complement(6829..7695)
                     /gene="rlmB"
                     /locus_tag="VHS06_12715"
     CDS             complement(6829..7695)
                     /gene="rlmB"
                     /locus_tag="VHS06_12715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX2989013.1"
                     /translation="MRTGQRGQSGSIATGRRQEHRPEGRSAAGGGGFHFPPDSELLYG
                     RNAVREALRAAKRVRRLLVAEGGERDEVVAESIRLAQKLSISPEMVSRERLDALVQDN
                     QGVVAVAAPFEYAGWGDLLDQLKAASKPPAVLMLDTLQDPQNLGTLLRTSEAIGLDAL
                     VLPKRRSVHVTPAVVRSSSGAVEHMSVARVPNLVRAAMDLKEIGFWVAGIDMDGDRLY
                     WDLDMSGPTALVLGGEDHGIGQLMKENCDFLVRLPMAGKVNSLNAAVAGSVVLYEMAR
                     QRKLSALPLGEA"
     gene            complement(7646..>7992)
                     /locus_tag="VHS06_12720"
     CDS             complement(7646..>7992)
                     /locus_tag="VHS06_12720"
                     /inference="COORDINATES: protein motif:HMM:NF020752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEX2989014.1"
                     /translation="DFNTAQALAVLFDLSRDINRLRTQEGYPNEALAPVQKKMMGLGA
                     VLGLKLEAPAESAAEKAADPFVQLLVELRGKLRSAKQWALADEVRDRLKELGVAIEDR
                     PEGTIWKYSDRT"
BASE COUNT         1525 a         2754 c         2192 g         1521 t
ORIGIN      
        1 cagactcaga ccgacagctt tggcttccaa ggggtcacgc tgcagagcgc gcagttcgag
       61 gatctggtgg ccacgatcca cctggtcagc ctcacggcag ccgtccaggg cctggacggc
      121 ccggtgtcgc aagatacgcg cctgtaagtc aagacttctc cactatcccg gggttggtct
      181 gcgaccaact ggtgaagccc ccggacaggt ttaccacccg atgcccgtga tagccgagga
      241 tcttacaggc gttgtaagag cgatacccgg cggcacagta gaccgtgata ggcccatcgc
      301 gacggatctt gtccagcgaa gaccgcagct cgtccagggg aacgttgatc gcccccggga
      361 tatgcccctc ctggtactcc tcctcggtcc gcacgtccag cagccgcagc ggcgcaccgt
      421 tcgcaatctt cccggcaaca tccgcagggg tgaccgcctc cacctctccc ctccactgat
      481 tggccgtcac gaaacccgcc atcacgacgg ggtccttggc ggaagagtag gggggcgcgt
      541 aggcgagatc caatccttct aggtcctcaa cggtcatgtt gccgtggatg gccgtcgcga
      601 tcacatctat cctcttgtcc actccctgcg gccccaccac ctgagcaccg aggacgcgac
      661 cgctcgttcg atccgccacc agcttgaagg tcatcttctg cgcaccgggg tagtagccgg
      721 cgtggtccag gggatggagg aaagacacca ggggatcgaa gcccgcagcc ttcgcctctc
      781 tctccccgag gccggtcttc ccagccgaaa gatcgcacac cttcacgatg ctcgtgccga
      841 tgacgccggg gaatcggctg tcgccgccgg cagcgttggc tcccgccacc ctgccctcct
      901 tgttggccgg accggccagc ggcagacgga cgcgccttcc ggtgatccgg ttcaccacct
      961 cggccacgtc gccgcaggcg aagacgtcgg cgtcgctggt cctcatgtgg tcatccacaa
     1021 ccacgccgcc gctctcaccg acctccagac ccgcatcccg tgccagggtc aactccggcc
     1081 gcacgccgat ggctaccacg gcaacctgcg ccggcaggcg ctcacctgat gccagccgaa
     1141 cgccctccag acgctcttcg ccgtcgaaac ccactacttc tgccccaagg tgcattctga
     1201 cgccgcgctc cgataggtga gcatccagga ggccggccat ctcaggatcc atttgaggca
     1261 gcatctgcgg aagcttctcc acgaggtgca cttcgagacc cagactcagt agcgcctcca
     1321 cggcctctat tccgatgaag ccgcccccga tcacgaccgc ctgcttcgct ccccggaagt
     1381 cgatccaccg gcgaagcgac tccgcctcag gtacggtcgt cagcgagaag acccccggac
     1441 gatctatgcc cggaatggga gggagtaccg gcctgccgcc cggcgcgagg atcaaccgat
     1501 cgtagggcac ccggcgacgc tcaccggtga gcaggttgag aacctccacc tcgtgctcct
     1561 cccggaggat cccggtgacc tcctggtgaa gctccacgtc gattcggaag cggtcggcaa
     1621 accgctccgg agtcatgata agcagatcgt cgagactcgg gatctcccca ccgacgtaat
     1681 acggaagtcc gcagttggcg aaagaaacgt agggaccccg ctcgaagatc actatctccg
     1741 caccctcgtc cgtgcgacga gccttggtgg cggcactggc accggcggca accccgccga
     1801 ccacgacgat tctcctgggt gtgctcattt ctgactgcaa cagcgatcca cctcgcggcc
     1861 acggcctcac gcgagcaggc gacttgcccg tcccggcgta cgaacgacca tggcgggata
     1921 ttgaatggga aagagcgcaa taatcgtaac catagaccga ttgcagtacg cgctcaaccc
     1981 ccacccgtca cagtataccc cataggggta tatctcggca aggcaagaca cggctgcgac
     2041 ggtatcttca gcgcataaag cagggcccct ggttcgcacc agaggcccca tccatgggag
     2101 ccgacgatgg gactcgaacc cgcaaccggt ggtttacaaa accactgctc tgccaattga
     2161 gctacgtcgg caagctattc cgcagcggcc accaacggca tcgccacgtt gatatcaact
     2221 agattctatc aaagcgtcgg gccgccgaca acccgcggat tcgactcggg agcccgaacc
     2281 cagagctcac tatccgccga tgcagtggag acagcaggag atcatcatca ccatgccgat
     2341 gctgaagggc acccaccaga agtggcggtt catccgggtc aactgcccct cgtggtgctt
     2401 caccacgcgg acaatgcgga tagtccgctg attgacccgc gcctgcaccg cgacctcgcc
     2461 ctccagggag tcgagtgcca gcaacgcccg acactcctgc agcgcatact ccagttcatc
     2521 acgatccgcc tggtagcaac tctggcgcca ggcctggagc gcagcatact ccagtcgcac
     2581 cagtcgctct ctaggactca agtcgggcga ctcgaaatcg tatggagaac gccagccacg
     2641 agggttcgcg acgttcatca gatcagcaac caaatctgcc agcctcattg ctgtcccccg
     2701 gttgccccgt ctgattcgcc tcccctcacc gtcaagagga ccaaaggagc gtcggtcttc
     2761 aacactccat cggcttcaag cacacagaca agagtctatt gcgatatatg ccaaccaaac
     2821 gccccgccaa gcgatccggc cccctcaggc gggcgtcttg ctcctattgt tccttcatcc
     2881 tcgagatgat cgcgtcggca aactgcgatg ttcccactgc agtcgggtcg tccggcgacg
     2941 gcttcatgtc gtaggtcacg ttccgcccct cggccaccac ggaggcaaat gccctctcca
     3001 gtcgatccgc cgcggcgaat tcgcccaggt gtctcagcat cagcatgccc gacagtatcg
     3061 tcgctgaggg attcaccttg ttcaggccag catacttcgg agcgctccca tgtgtcggct
     3121 cgaagatcgc gacgttgccc tctccgaagt tgccgccggg tgccaccccc aggccgccca
     3181 caagaccggc ggccagatcc gagatgatat cgccgaacag gttgggcaac accagaacgt
     3241 cgtagagctc cggcttctgc accagctgca tgcacatgtt gtccacgatc cgatcgtcga
     3301 actcgatatc cgggtactcc ttcgcgacct ccgacgcaac ctgaaggaac aacccatcgc
     3361 tgaacttcaa tatgttcgcc ttgtggaccg ccgtcacctt gcgccgcttg ttcgcccggg
     3421 catattcgaa ggcccacttc actacacgcc gcgtaccggt aacagagatg gacttgatgc
     3481 tgattccaga gtcctcgggg atctgtccgg ccccggtctc agcggccaat gctatcagcc
     3541 gcttcgcccc ttccgagcct ttctggaact ctacaccggc atacaggtcc tccatgttct
     3601 cccgaaccac caccaggtcc acgttctggt agcggctgcg aacacccttg tagctcttac
     3661 acggcctcag gcaggcgtac agtcccaggc tctttcggag cgccacattc acgcttcgga
     3721 acccttttcc aataggcgtg gtaatcggtc ccttcagcgc caccttgttc ttcttgatgg
     3781 actcgatgac ctggtccggc aggggagtcc catacttctc caggacgtct acgcccgctt
     3841 cctggacgtc ccagtcaaac tgagtcccgg tggcttccag cacccgtctg gtcgcctcga
     3901 cgatctcagg gcccgtcccg tcacccggga tcagcgttac gcgatgcgcc gccatcgttc
     3961 ttactccctt cgctcatccg tcgagaacta caacagtgag caagcaaccc cgcctaccta
     4021 ccgctcctcg tagagtggtt tcttcacctg ctgagtagtt ccagtatacc gctgagcaac
     4081 cgactcaaca aggttaacgg aatcatccca aaactaagag aagcctcagg tctcaaggta
     4141 gagacgctga ggcttctcca catcttcacc ctgctgctaa gccttcgcgt acttcgcagg
     4201 gccgctctcg tagagatcct tgccggtcga gtcgacaatc acgatggcgg gaaaatcctc
     4261 cacggtgatc ttccgaacgg cctcggtccc gaggtctgga taggccacga cctcggcagc
     4321 cttgatgcag agggaaagca gcgccccgct tcctccggta gccccgaagt agacgcagcc
     4381 gttcctgacc atggcgtcct tgacctcgcg gctccgcagt cctttcccca ccatccccag
     4441 caagccatgg tccatcagga ggggcgcgta cttgtccatc cgatagctcg acgtagggcc
     4501 ggccgaacca atgggacgcc ccggcgccgc aggcgtcggc ccagtgtaga agatgcactg
     4561 gtcggcgatg ctaaccggca gttccttccc ctgctggatc agttcgaaga gtcgcttgtg
     4621 agcggcatcc cgcccggtga gcatggttcc tgagaggaga accaggtcgc ccgccctcag
     4681 cttcttgacg tcttcccgtt tgagaggcag agttagccta atcgaatcag tcatggttct
     4741 ctcctccaag caggaccgca gtcttgtgtc tgtaggcgtg gcaccccagg ttgattgcca
     4801 gcggtaggca ggctatgtgg gttgggtagg tttcgatgtg gacggccagg gcagtgaccg
     4861 ttccacccag cccagcaggc cctattcccg tcttgttaac ctcttccaac aactccgcct
     4921 cgagcgccgc aatcctcggg tccgggttgg gctcccccag gttccgaagc agcgaccgct
     4981 tcgccaggta ggcgcacttc tcgaagttgc cgcctatccc aacgccgacc actatcgggg
     5041 gacaggggtt gggtcccgcc cgccggacgg tgtccacaac ctccttcttg atgccttccc
     5101 agccggaaga cggcgtgagc atgaccaggc ggctcatgtt ctcgcttccg gcgcccttag
     5161 gcacgatact gatgaccagt tcgtccttgt cgctctcttc gatgtggatg accggaggag
     5221 tattgtcgcc gctattcttc cgctcgaata gaggctcggt gacgacgctg gcgcgcagat
     5281 aaccctcggt gtaacctctg cgcacgccct cggtgatggc gccggtcagc gtgcctccct
     5341 ctatcgtgac tcgcggcccc ttccgcacga agatagcggc aacccctgtg tcctggcagt
     5401 aggggatgtg ttccgcgacc gcgacctcgg cattccgcaa caactccttg aagatcgcct
     5461 ttccctgggg ggattcctcc cgatccaatg cccgccggaa ggagtccacc acatcccgcg
     5521 gcaactcgta agccgcggcg atgcagagat cacgcacctt tgccgtgatc tcagacgaag
     5581 ctacactagg caccttatcc cacctccttg tggtaatgac agagactcag agggactata
     5641 ggttcttggg ggaactctgg gggacgaagg aacgggggga gccccagggg tccagcgatg
     5701 tgggggattc ggggatttcg ttctccccga gatcccccac ctgcttcttg tctacaaccc
     5761 gaccgaggtc gctgcctcct tgagtacctt gaaggaattc cggaactccg catcctcctt
     5821 tgctgagaag tgcggatcca gaattgcctc ccttccgttg cgccccacga tgcagggcag
     5881 ggagatggtc attccggcga ggtcgccaaa gtgaccatcg ataagcgacg agatcggcag
     5941 taccttcttc tggtcgttgc gccacgctcg aaccaccgag acgatggacg gagccactga
     6001 gtagaaggtg ccgccctttc gcttgatcac ctccgcccca ccaaaccgtg cggcagtgat
     6061 caactcctcc aacttctgat cattgtaccc cgggtaatcc ttgagcggta tgcccgcgac
     6121 ggtcgcaccg gacaccaccg gaaccatgct gtctccgtgc tcgcccatca tgtaggccac
     6181 gacctggtcg ggcgaaacgc ccagccattc gccaatcaaa gagcggaggc gagtagtgtc
     6241 caatacggta ccgaggccaa acaccttgtt ggatgggaag cccgaagtct tgagggcctg
     6301 ataggtcagc acgtcgaccg ggttggagac catcaggatg aaggtgtcgg ggttgtgctt
     6361 caggacacct tccaatatgc cccccatcaa ctctgtgttc ttcttcagca ggtccagtct
     6421 ggtgtcaccc ggcttcctcg ggataccggc agtgatgatc accatgtcgg atccttcggt
     6481 cacgtcgtag tcaccagcta cgatcttgca gttctgggtg agtgcgagcg cgtgtcgaag
     6541 atccagcgcc tcaccttccg cgcggggctt gtccacgtcg acgatcgcca tctccgtgac
     6601 gccgccctcg agcagcaaag tgtacagggt ggtggacccc acccttccgg cgccaatcat
     6661 ggagatcttc atgggtcgcc ctccttgttg cagtagtcag atccagtgtt ggacacactt
     6721 gactatttta gtctgcacgg cgacggctcc acaagcatca acttcctggc gcattcgcca
     6781 gcggcagggg agaaagccct cacgccgtag gcatgccttc cggcatgtct acgcctctcc
     6841 gagaggcaga gcggacagct tccgctgccg cgccatctcg tagagcacta ccgatccggc
     6901 aacggccgca ttcagtgagt tcaccttccc cgccataggc agtcgcacca ggaagtcgca
     6961 gttttccttc atcaactggc cgatgccgtg gtcctcgccg cccaacacga gcgccgtggg
     7021 accggacatg tccagatccc agtacaggcg atcaccgtcc atatcgatcc ctgcaaccca
     7081 gaagccgatc tccttcaggt ccatggcggc acgcacgagg ttcggcaccc tcgccacaga
     7141 catgtgctcg accgctccag aggatgagcg gaccaccgcc ggcgtaacat gaaccgatcg
     7201 tcgcttggga agcaccagcg cgtccagccc aatcgcctct gaagtgcgca gcagcgtgcc
     7261 gagattctgg ggatcctgaa gagtatccag catcagaacc gccggaggct tgctcgccgc
     7321 cttcaactga tccagcaggt ctccccaccc tgcgtactcg aagggggcag ccacagccac
     7381 caccccctgg ttgtcctgca ccagggcatc gagccgctcc cgagacacca tctccggaga
     7441 gatgctgagc ttctgcgcaa gtcggatcga ctccgccaca acttcgtcgc gctcaccgcc
     7501 ctcagcaacc agaaggcgcc gcacacgctt ggccgcgcgc agcgcctccc ttaccgcgtt
     7561 ccttccgtag agaagctcgc tatcgggcgg gaagtggaag ccccctcccc ccgccgccga
     7621 ccggccctcc ggcctgtgct cctgcctacg tccggtcgct atacttccag attgtcccct
     7681 ctggcctgtc ctcaatggcc actcctagct ctttcaatcg atcccgcact tcgtcggcca
     7741 acgcccactg cttggcggat cgcagcttcc cacgcagctc tacgagaagc tgaacgaagg
     7801 ggtcggcagc cttctcggcc gcactctccg ccggcgcttc gagcttcaat cccagcaccg
     7861 cgcccagtcc catcatcttc ttctgaacgg gggccagcgc ctcgttcggg taaccctcct
     7921 gggttcgcag cctgttgatg tccctggaga gatcgaatag caccgccagg gcctgggcgg
     7981 tgttgaagtc gg
//
LOCUS       DATALS010000571         7471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexota bacterium isolate SMAG_U6970 k141_89656,
            whole genome shotgun sequence.
ACCESSION   DATALS010000571 DATALS010000000
VERSION     DATALS010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955586
            Sequence Read Archive: SRR12844109
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexota bacterium (soil metagenome)
  ORGANISM  Chloroflexota bacterium
            Bacteria; Chloroflexota.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:54:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,550
            CDSs (total)                      :: 2,525
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 7 of 53
            Pseudo Genes (incomplete)         :: 45 of 53
            Pseudo Genes (internal stop)      :: 2 of 53
            Pseudo Genes (multiple problems)  :: 1 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Chloroflexota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89656"
                     /isolate="SMAG_U6970"
                     /isolation_source="soil"
                     /db_xref="taxon:2026724"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="VHS06_12725"
     CDS             <1..1639
                     /locus_tag="VHS06_12725"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEX2989015.1"
                     /translation="GVRAIAAGDFHSLALKSDGTVYAWGDNFFGQLGNGIASSNTPVQ
                     VNGLSGVIAISAGDFHNLALKSDGTVYSWGDNFYGQLGNGGSGNYSNSPAQVNNLSGV
                     TAIAAGTSHSLALKSDGTLRAWGHNNAGQLGDGTATDSNTPGQVNSLSEVRAVAAGGD
                     HSLALKPDGTVLAWGDNFYGQLGDGSTTNSKTPVHVSGLSGMSAIAAGTGHNLALKSD
                     GTVWAWGYTGDGQLGNGNTANNNTPVQASGLSGMSAIAAGDYHSLAIVSSPLLEPSPT
                     SVPSSGGNDGWRPWPTPTPNANTGEGYTNPAVGGSMTSRDARVTVEMAGGTVDRLTKL
                     WYEKQCTTEIKPPAGESYLGCPFDLRAVDAADERVNLSRFGKSLQIAVRYTDEEARGL
                     TPSSFHLAYYDGTSWVPLPSRVDPEGKLVTAPTDHFTLFSLLGSSAPMVPMQSEISGR
                     VYYDRNGNGVFDDEDFPIAKAGVKIESGDWYAFTTTEWDGRYAFPCLRESKYTVSLEV
                     GPEWAFTTPNRVVDIPVTGRVDSTQTGVDFGMWYRVP"
     gene            complement(1768..3090)
                     /locus_tag="VHS06_12730"
     CDS             complement(1768..3090)
                     /locus_tag="VHS06_12730"
                     /inference="COORDINATES: protein motif:HMM:NF022822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stealth family protein"
                     /protein_id="HEX2989016.1"
                     /translation="MRCVAQMRKEILEARYFQYVRVGHDHRRMEFLPMNRIGGGRARL
                     EQTWHLLQRGDEIVLAIAGQEGVVCKLFPQDDGSWKGKWLLYEGMPVELKPDRGRPPV
                     ATTGTSITGATSEHEPIDAVYLWVDGADPLFLADLHRWATECGATFDPLRLAPGRFRD
                     NGELRYSLRSLEAYAPWIRHVHIVTNGQVPSWLDATNHGVSVISHDTVFPDRTHLPTF
                     NSHAIELQLHRIPNLTRRFIYFNDDLFLGRPVVPEDFISPEGVQTVYLENWLLPQELH
                     LGAVHDRAYAYTQRLLNDRLMERSSRRGIAHVPQMFDVDLVQEVQYLWRLQVEETSAH
                     RFRSPRDVALRILYYYYALESRAHQSSCATRTLYSPSEDYHFLQMEESIPAMVESFNE
                     LMFQRPKFFCVNDDLGDSEGAEIVLQAFDAFLEEYFPRPSSFEKGRAH"
     gene            3324..4760
                     /locus_tag="VHS06_12735"
     CDS             3324..4760
                     /locus_tag="VHS06_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HEX2989017.1"
                     /translation="MLHSRRPRRETGYQLTEAGDDTVDLTRIDGGMAIRLNATSALIW
                     VLCDGERNVADIIDLLRTAFPNSDRDVGADVRGTILALVEAGVVTWEEGFAPPRVEVP
                     ILLYHKVADISAPYSSIWVTANTFARQMAALHAYGYTTVSLEDYMLYRQGLAVPPDNP
                     VILTFDDGYECLWNIVRPVLDRYGYKATIFLLTGHTGDSLREDTRWDQVEAAYPAKMM
                     LWSEAIALLADGHYLESHTRWHSQLSTSSESWSRTEIRESTEEMQSRLGIAPRFFAYP
                     YGDGAGMPQLAAVLQSEGYLGAVASQGGIANTRDSDLWALPRIKITEDNSTMLDPAHP
                     DDFFMRQVDPSFPLPAIEVEAPSITDSVGGSDRSSRSYHPGEAVILTVRARNGGMASD
                     VRISLTISRSGEKSVLPIHVGTASADNERTHMGSGKSRSFVVPWRIPEHLSPGLYDVE
                     FTVYDVHRVLVFFSSRWERWLNVEAAGK"
     gene            4778..5062
                     /locus_tag="VHS06_12740"
     CDS             4778..5062
                     /locus_tag="VHS06_12740"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX2989018.1"
                     /translation="MLDTRRPRRTPGYLVGPVPSSNADFVLADPTGRCTLRVNRSVGL
                     IWGLCDGHRAPREIVRLLLDAYPERADNMDSDVRETLIALTEYGAIEWVP"
     gene            5180..5707
                     /locus_tag="VHS06_12745"
     CDS             5180..5707
                     /locus_tag="VHS06_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX2989019.1"
                     /translation="MDEPNGKPQDQPDGSTPRSIGRRQLLKALGATAGTFAAMAFLPD
                     RWVKPAVAQVAGFRIDDLSVSEGVVGFAYHDLQHEVDDSTTWMHAEVDSASGQIINTQ
                     YNSLQNLRAQLGVRRHGGPAGGSIQFFLNGLTPNGLPQCGVNSSWNLGIYLRAGATNA
                     SWRYSNTLNTHICQA"
     gene            5951..>7471
                     /locus_tag="VHS06_12750"
     CDS             5951..>7471
                     /locus_tag="VHS06_12750"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2989020.1"
                     /translation="MANILIYTEGTLGDHLPYIALGQALSARGHRVRFAINKAMHKYA
                     QLSGLEAVALTDTERGPEEARKNAWAWDHWHQVDLDSCYRAEPLAQDHYVTRARELIE
                     LCHDADLLIATSIRTLGYVAHAATGIPWLTASMNPYSFWQPVSLEEREAWLQGRLQEY
                     ENLKGLLAYTFAQLGLDQPVPNFSKAWLYARHVILASSTHFGRPDLEQLQPYSSLDMT
                     GFWFYQDPGWENWEPSDELRRFCDRRPMVLSFSSQPLADPRSVLATHVEAAQHLGLPL
                     LIQRGWAGFTEEDLPPDSDPSQLLFTDFLPHDWLFSHASCAIQHGGIGSIARALRNGC
                     PLLIEPYGNDQLYNASRVVQLEMGAAMHPFKMTTDGLVEVLSDKVLSSDCRKRVQALG
                     ATLRIEDGLGSACRAVETYLDRVTSGKEESTAQAVPLLPNRYAPPLDAPMARPEAVAT
                     ARPSPQPIPKMVHQTWRDANIPPVYTRFRDGWQRHHPDWNHYLWTDTDNREFLRRHY"
BASE COUNT         1495 a         2303 c         2291 g         1382 t
ORIGIN      
        1 cggggtaagg gccatcgccg cgggcgattt ccacagcctg gcactgaagt cggacggcac
       61 cgtctacgca tggggagaca actttttcgg ccaattaggc aacggtatag ccagcagcaa
      121 cacccccgta caggtaaacg gtctcagcgg ggtgatcgcc atctcggcgg gcgatttcca
      181 caacctggca ttgaagtccg acggcaccgt ctactcgtgg ggagacaact tttacggcca
      241 gttggggaat gggggctccg gcaattacag caacagcccc gcacaggtga acaacctcag
      301 cggcgtgacc gccatcgccg cagggaccag ccacagcctg gcgttgaaat ccgacggcac
      361 cctccgggcg tgggggcaca acaatgccgg ccagttgggt gacgggaccg cgaccgacag
      421 caacacgccc ggacaggtga acagcctcag cgaagtgcgc gctgtcgcag caggcggtga
      481 ccacagcctg gcgttgaagc ccgacggcac cgtcttggca tggggagaca acttctacgg
      541 ccagttggga gatgggtcta cgaccaacag caagactcca gtacatgtga gcgggctcag
      601 cgggatgagc gccatcgcgg cgggcaccgg tcacaacctg gcattgaagt ccgacggcac
      661 cgtctgggcg tggggataca ccggtgatgg ccagttggga aacgggaaca cagccaacaa
      721 caacaccccc gtacaggcga gcgggctcag cgggatgagc gccatcgccg cgggcgacta
      781 ccacagcctg gcgatagtgt cgtctcccct cttagagccg tctccgacct cagtgccgtc
      841 ttcgggtggg aatgatggat ggcgcccatg gcccacaccc accccgaacg caaacacggg
      901 agaggggtat acaaacccgg cagtgggagg atcgatgacc tcccgagacg ccagggtgac
      961 ggtggagatg gccggcggga cagtggaccg gctcacgaag ctgtggtacg agaagcagtg
     1021 cacgacggag atcaagcccc cggcaggaga gagctacctg ggctgtccct tcgacctgag
     1081 ggccgtcgac gctgcggacg agcgagtgaa tctgagccgt ttcgggaagt cgctgcagat
     1141 cgcggtccgg tacacggatg aggaggcacg ggggctgact ccttcctcgt tccatctggc
     1201 gtattacgac ggaacgagtt gggtgcccct gccgtcgagg gtggatcccg agggaaagct
     1261 cgtgacggca ccgaccgacc acttcacgct gttctcgctg ctgggctcat cggcgcccat
     1321 ggtgcccatg cagtccgaga tctcggggag ggtgtactac gatagaaacg ggaacggggt
     1381 gttcgacgat gaggacttcc cgatcgccaa ggccggcgtg aagatcgagt ccggcgactg
     1441 gtacgccttc acgacgaccg agtgggatgg tcgctacgca ttcccctgct tgagggagag
     1501 caagtacacc gtctcactgg aggtggggcc cgagtgggcc ttcacgacgc caaaccgggt
     1561 ggtggacatc ccggtgaccg ggcgggtcga ttcgacgcaa acgggcgtgg acttcggaat
     1621 gtggtaccgt gtgccatagt ccgcaaccgt cgcccggtgt tctactgttg tccggctcag
     1681 acggcacttt cctgcgagca gctttccccg aatgcggtca gcctttccat tcgatgagcg
     1741 gaaatggaac cgaccgggga aagctgctta gtgggcccgc cccttctcga agctcgacgg
     1801 gcgagggaag tactcctcga ggaaggcgtc gaaagcctgc aagacaatct ccgcaccctc
     1861 cgagtctccc agatcgtcgt tgacgcagaa gaacttcggc cgctggaaca tcaactcgtt
     1921 gaagctctcg accatcgcgg gtatgctctc ctccatctgc aggaaatggt agtcttcgga
     1981 tggactgtac agcgtcctgg tggcgcagct cgattggtga gcgcgggact ccagggcata
     2041 gtagtaatac agtattcgaa gagcaacgtc gcgcggcgaa cggaaccggt gcgcggaggt
     2101 ctcctccacc tggagcctcc agaggtactg aacctcctgg acgagatcca catcgaacat
     2161 ctgcggtacg tgggcaatac cccggcgact cgatctctcc atcagtcgat cgttcagcaa
     2221 tcgctgagtg taggcatagg cccgatcgtg cacggcgccc agatgcaact cttgaggcaa
     2281 cagccagttc tccagataga ccgtctgcac cccttccgga ctgatgaagt cctcgggaac
     2341 caccggcctc cccaagaata ggtcgtcgtt gaagtagatg aaacgacgag taaggttggg
     2401 gatccggtgc agttgcagct cgatagcgtg ggagttgaag gtggggagat gcgtgcgatc
     2461 gggaaagacg gtgtcatggg agatcaccga taccccgtgg ttggtggcat ccagccaact
     2521 cggaacctgg ccgttggtta cgatgtggac gtgcctgatc cacggggcgt aggcttccag
     2581 cgagcgtagc gagtatctta gctccccgtt gtcgcgaaag cggccgggag ccaaccggag
     2641 gggatcgaag gtcgctccgc actcagttgc ccaccggtga agatctgcta agaacagggg
     2701 atccgccccg tccacccaga ggtacactgc atcgatcggc tcatgctcgg atgtggcacc
     2761 cgtgatcgac gtacccgtcg ttgccaccgg cggccgccct ctgtcgggtt tcagttcaac
     2821 cggcattccc tcatatagga gccactttcc tttccaactg ccgtcgtctt gagggaagag
     2881 cttgcagaca accccttcct ggcccgctat cgcgagcact atctcgtcac cccgttggag
     2941 caggtgccag gtctgctcga gcctcgccct accgcctccg attcggttca tgggcaagaa
     3001 ctccattcgt cggtgatcgt ggccaacgcg aacgtattgg aaatacctgg cctcgaggat
     3061 ctccttccgc atctgtgcca cacaccgcat ggtgactcga cctcatctta tttctgaatg
     3121 gagatgaccg catccgcccc tgcagaggat cggcaaaccg acgcattcta tcacctcatg
     3181 cttcaacatc aatctgacga accgatagtc gtggttgttg gcgcggaacc gtgccttgac
     3241 aggtaatccg gatgcttcta cgatcaacat cagatgtccc ctcgaggttg tctacgccat
     3301 ccttcctcat ggtgaaggcc gtcgtgctcc actcaaggcg cccacggcgc gagaccggat
     3361 atcagctcac ggaggccgga gacgataccg tcgacctgac acggatcgat ggcggcatgg
     3421 cgattcggct caacgccacg agtgcgctga tttgggtcct gtgcgatggc gagcgcaatg
     3481 tggcggatat catcgacctg ctgcgcaccg cgttccccaa ttccgaccgg gatgtgggcg
     3541 ccgacgtgcg cgggacgata ctcgccctgg tcgaagccgg ggtggtgacg tgggaagagg
     3601 ggttcgcccc tccacgtgtc gaggttccca ttctcctgta ccacaaggta gccgatatct
     3661 cagcccccta tagctccatc tgggtgaccg cgaatacatt cgcgcggcag atggcagcgc
     3721 tgcacgccta cggctacacg accgtatccc tcgaagacta tatgttgtac cggcaggggc
     3781 tcgcagtccc gcccgacaac ccggtgatcc tgaccttcga cgatggatat gagtgcctct
     3841 ggaacatagt caggccggtc ctcgaccgat acgggtacaa ggccacaatc ttcctgctca
     3901 ccgggcacac cggcgattcc ctgcgagagg acacgaggtg ggatcaggtg gaggctgcct
     3961 accccgcgaa gatgatgctc tggtctgagg ccatcgcgtt gctggcagat gggcattacc
     4021 ttgaatccca cacgcgatgg cattcacagc tctccacgag tagcgagtcc tggtccagga
     4081 cggaaatccg ggagtcaacc gaagagatgc aatcgcgtct gggcatcgcg ccccgcttct
     4141 ttgcatatcc ctatggcgac ggcgctggca tgccacaact ggcagccgtt ctgcagagcg
     4201 aagggtacct gggggctgtg gccagccaag gtggcattgc caacacacgg gactcggatc
     4261 tctgggcatt gccgcgcatc aagatcaccg aggataactc gaccatgctc gaccctgccc
     4321 atcccgacga tttcttcatg cggcaggtag atcccagctt tcccttgcct gcaatcgagg
     4381 tcgaagcccc gtccatcacc gattcggtcg ggggctcgga ccgttcgagc cgttcgtacc
     4441 acccaggcga agccgtgata ctcacagtcc gcgcaaggaa cggcggtatg gcctccgacg
     4501 tgcggatctc gctgaccatc agccgttcag gcgagaagtc cgtgttgccc atccacgtcg
     4561 ggaccgcctc ggcagacaac gaaaggacgc acatggggtc gggcaagtcg cgttccttcg
     4621 tggtcccctg gagaatcccg gagcatttgt cccccgggct gtatgacgtc gagttcaccg
     4681 tctacgacgt ccatcgcgtc ctggtcttct tctcctctcg gtgggagcga tggctgaacg
     4741 tcgaagccgc gggtaagtga gataagagga gggcaggatg ctagacacga gacgaccgag
     4801 gcgaactccc ggctatctgg tggggccggt gccctcctcg aacgccgatt tcgtgctcgc
     4861 tgatcccacc gggcgctgca cgttgcgcgt caatcgcagt gtgggcctca tctgggggtt
     4921 gtgcgacggc catcgcgcgc ctcgggagat cgttcggctg cttctggacg cgtatccgga
     4981 gagggccgac aacatggatt ccgacgttcg ggagaccctt atcgcgttga cggagtacgg
     5041 cgcaatcgag tgggtgccgt agctacttca tcttgactcc gtctttgttg atggagaata
     5101 atgtgccggc aggcgagagt ctcttcggat aggcccgagt ttcgaatggg ctatgtgtag
     5161 agtggaggag acgcacccca tggacgaacc aaacggtaag cctcaagatc aaccggacgg
     5221 atcgacacca cggtcgatag gccgccggca actcctcaaa gccctgggtg cgacggccgg
     5281 cacattcgcg gccatggctt tcctcccaga tagatgggtc aagcccgccg ttgcacaggt
     5341 agcaggattc cgtatcgatg atctctcggt atccgaaggg gtagttggtt tcgcctacca
     5401 tgatctgcag catgaggttg atgacagcac cacctggatg catgcggagg ttgattctgc
     5461 tagcggccag ataatcaaca cgcaatacaa ctcattgcag aatctccgtg cacagcttgg
     5521 cgtgcgtcgc catggtgggc cagcaggcgg aagcatacag ttcttcctga acgggctcac
     5581 ccccaacggc ttgccgcaat gcggcgtcaa cagttcctgg aacttgggca tctatctgag
     5641 agccggcgcg acaaacgctt cctggcggta ttcgaacacg ctgaacactc acatctgcca
     5701 ggcctagtcg aatccgttgg gtcgctgagt gtggtatcca gaccatttcg tagagcttcg
     5761 atgatcgccg gcgggcccga ggggtagatc gcatcgcggt ctacggggat ggctcctttg
     5821 gcgatcgact tcccttcgag accttggggt agctcctcgg cgtgcgccgc catcgcgtcg
     5881 tgtctcacct tgcctcgtct tctaacgaag ccggcccgaa cccaaggcta tgctactggg
     5941 ggttaggctc gtggcgaaca tcctcatcta caccgaaggg actctaggag atcaccttcc
     6001 atacatagct ctcggacagg ctctgagtgc ccgcggccat cgcgtacggt ttgcgataaa
     6061 caaggccatg cacaagtacg cgcagctctc ggggctagag gcggtggcgc tcaccgacac
     6121 ggagcggggc ccggaggaag ctcggaagaa cgcctgggcg tgggatcact ggcaccaggt
     6181 cgatctcgac tcctgctacc gggccgaacc tcttgcccaa gaccactacg taacccgggc
     6241 tcgggagctg atcgagctgt gccacgatgc cgacctcttg atcgccacct ccatccgcac
     6301 cctgggctac gtcgcccatg ccgccacagg gattccctgg ctcaccgcat ccatgaaccc
     6361 ctacagcttc tggcagccgg tttccctgga ggaacgagag gcatggctgc aagggcggct
     6421 gcaggagtac gagaacctca aggggctcct tgcctacaca ttcgcgcagc ttggtctcga
     6481 ccaacctgtg cccaacttct ccaaggcgtg gctttatgcc aggcacgtga tcctggccag
     6541 cagtacccac ttcggccgcc ccgacctgga gcagttgcag ccctactcca gcctggatat
     6601 gacgggcttc tggttctacc aggaccccgg ttgggaaaac tgggagccgt cggatgagtt
     6661 gcgacgcttc tgcgaccgcc ggccgatggt tctcagcttc agcagccagc cgctggcgga
     6721 tcctcgctcc gtgctggcaa ctcacgtcga agccgctcag cacctggggc taccgctgct
     6781 gatacaacgg gggtgggccg gcttcaccga ggaggatctg ccgcccgaca gcgatccgag
     6841 ccaactgctg ttcaccgact tccttcccca cgactggttg ttcagccacg catcctgtgc
     6901 catccagcat ggaggcatcg ggtccatcgc ccgagccctg cgcaacggct gcccgctcct
     6961 gatcgagccc tacggcaacg atcaactcta caacgccagc agggtagtgc aactggagat
     7021 gggggcggcc atgcacccct tcaagatgac aaccgacgga ctagttgagg tcctttccga
     7081 caaggtgctg agttccgact gccggaagcg ggtgcaagcc ctgggagcga cactgcggat
     7141 agaggacggg ttgggatcgg cctgccgggc ggtggagaca tacctggatc gggtaacctc
     7201 cggcaaggaa gaaagcaccg cgcaagcagt ccctctactg cccaaccgat acgcgccgcc
     7261 actggacgcc ccgatggcaa gaccggaggc tgtcgcaacc gcgcggccgt ctcctcagcc
     7321 gatccccaaa atggtgcacc agacgtggag ggacgcgaac attccacccg tctacacccg
     7381 gttccgagat ggctggcaga gacaccaccc agattggaac cactacctgt ggaccgatac
     7441 ggacaaccgg gaattcctcc gcaggcacta c
//
